Claims
- 1. A conjugate molecule comprising:
a ligand; a polymer spacer; a polymer carrier; and a therapeutic agent, wherein the ligand is bonded to the polymer spacer, the polymer spacer is bonded to the polymer carrier, and the polymer carrier is bonded to the therapeutic agent.
- 2. The molecule of claim 1, wherein the ligand is covalently bonded to the polymer spacer, the polymer spacer is covalently bonded to the polymer carrier, and the polymer carrier is covalently bonded to the therapeutic agent.
- 3. The molecule of claim 1, wherein the polymer carrier is bonded to the therapeutic agent with a linker.
- 4. The molecule of claim 1, wherein the ligand is an antibody, an antibody fragment, a peptide or a protein.
- 5. The molecule of claim 1, wherein the ligand is selected from the group consisting of C225, Herceptin, Rituxan, a phage library antibody, anti-CD, DC101, an antibody to integrin alpha v-beta 3, LM609, an antibody to VEGF, an antibody to VEGF receptor, F(ab′)2, Fab′, ScFv fragment, c7E3Fab, a growth factor, VEGF-A, VEGF-B, VEGF-C, VEGF-D, PDGF, Angiopoietin-1, Angiopoietin-2, HGF, EGF, bFGF, cyclic CTTHWGFTLC, cyclic CNGRC, cyclic RGD-4C, annexin V, an interferon, a tumor necrosis factor, endostatin, angiostatin and thrombospondin.
- 6. The molecule of claim 1, wherein the ligand is an antibody.
- 7. The molecule of claim 1, wherein the ligand is a monoclonal antibody.
- 8. The molecule of claim 1, wherein the ligand is C225.
- 9. The molecule of claim 1, wherein the ligand is Herceptin.
- 10. The molecule of claim 1, wherein the ligand is c7E3Fab.
- 11. The molecule of claim 1, wherein the ligand is annexin V.
- 12. The molecule of claim 1, wherein the polymer spacer is selected from the group consisting of polyethylene glycol, a polyamino acid, polytyrosine,
polyphenylalanine, dextran, a polysaccharide, polypropylene oxide, a copolymer of polyethylene glycol with polypropylene oxide, polyglycolic acid, polyvinyl pyrolidone, polylactic acid and polyvinyl alcohol.
- 13. The molecule of claim 1, wherein the polymer spacer is polyethylene glycol.
- 14. The molecule of claim 13, wherein the polyethylene glycol has a number average molecular weight of about 1,000 daltons to about 100,000 daltons.
- 15. The molecule of claim 1, wherein the polymer carrier is selected from the group consisting of poly(1-glutamic acid), poly(d-glutamic acid), poly(d1-glutamic acid), poly(1-aspartic acid), poly(d-aspartic acid), poly(d1-aspartic acid), polylysine, a polysaccharide, polyhydroxypropylmethacryamide, dextran, poly(hydroxypropylglutamine), poly(hydroethylglutamine), hyaluronic acid, carboxymethyl dextran, polyacrylic acid, chitosan, and copolymers thereof.
- 16. The molecule of claim 1, wherein the polymer carrier is poly(1-glutamic acid).
- 17. The molecule of claim 16, wherein the poly(1-glutamic acid) has a number average molecular weight of about 1,000 daltons to about 100,000 daltons.
- 18. The molecule of claim 1, wherein the therapeutic agent is a chemotherapeutic agent.
- 19. The molecule of claim 18, wherein the chemotherapeutic agent is Adriamycin.
- 20. The molecule of claim 18, wherein the chemotherapeutic agent is paclitaxel.
- 21. The molecule of claim 1, wherein the therapeutic agent is selected from the group consisting of Adriamycin, daunorubicin, paclitaxel (Taxol), docetaxel (taxotere), epothilone, camptothecin, cisplatin, carboplatin, etoposide, tenoposide, geldanamycin, methotrexate and maytansinoid DM1, 5-FU, and gadolinium-DTPA.
- 22. A composition comprising a nanoparticle, said nanoparticle comprising a plurality of the conjugate molecules of claim 1.
- 23. A composition comprising the conjugate molecule of claim 1 and a pharmaceutically acceptable carrier.
- 24. The composition of claim 23, wherein the polymer carrier is bonded to the therapeutic agent with a linker.
- 25. The composition of claim 23, wherein the ligand is an antibody, an antibody fragment, a protein, or a peptide.
- 26. The composition of claim 23, wherein the ligand is an antibody.
- 27. The composition of claim 23, wherein the polymer spacer is PEG.
- 28. The composition of claim 23, wherein the polymer carrier is poly(1-glutamic acid).
- 29. The composition of claim 23, wherein the therapeutic agent is a chemotherapeutic agent.
- 30. The composition of claim 29, wherein the chemotherapeutic agent is Adriamycin or paclitaxel.
- 31. A method for selectively delivering a therapeutic agent to a target tissue in a patient comprising administering a conjugate molecule to the patient having said target tissue, wherein the conjugate molecule comprises: a ligand with affinity for the target tissue; a polymer spacer; a polymer carrier; and a therapeutic agent, wherein the ligand is bonded to the polymer spacer, the polymer spacer is bonded to the polymer carrier, and the polymer carrier is bonded to the therapeutic agent.
- 32. The method of claim 31, wherein the polymer carrier is bonded to the therapeutic agent with a linker.
- 33. The method of claim 31, wherein the ligand is an antibody, an antibody fragment, a protein, or a peptide.
- 34. The method of claim 31, wherein the ligand is an antibody.
- 35. The method of claim 31, wherein the polymer spacer is polyethylene glycol.
- 36. The method of claim 31, wherein the polymer carrier is poly(1-glutamic acid).
- 37. The method of claim 31 wherein the therapeutic agent is a chemotherapeutic agent.
- 38. The method of claim 31, wherein the administering step comprises intravascular, intraperitoneal or intramuscular injection.
- 39. The method of claim 31, wherein the patient is a mammal.
- 40. The method of claim 31, wherein the patient is a human.
- 41. The method of claim 31, wherein the target tissue is selected from the group consisting of a tumor, an inflammatory tissue, an infectious tissue, a reparative tissue and a regenerative tissue.
- 42. The method of claim 31, wherein the target tissue is a tumor.
- 43. The method of claim 42, wherein the tumor is a solid tumor.
- 44. The method of claim 42, wherein the tumor is breast cancer, ovarian cancer, colon cancer, lung cancer, head and neck cancer, brain cancer, liver cancer, pancreatic cancer, bone cancer, prostate cancer, lymphoma or leukemia.
- 45. A method of treating a patient having a diseased tissue, the method comprising administering a therapeutically effective amount of a conjugate molecule to the patient, wherein the conjugate molecule comprises: a ligand with affinity for the diseased tissue; a polymer spacer; a polymer carrier; and a therapeutic agent, wherein the ligand is bonded to the polymer spacer, the polymer spacer is bonded to the polymer carrier, and the polymer carrier is bonded to the therapeutic agent.
- 46. The method of claim 45, wherein the polymer carrier is bonded to the therapeutic agent with a linker.
- 47. The method of claim 45, wherein the ligand is an antibody.
- 48. The method of claim 45, wherein the polymer spacer is polyethylene glycol.
- 49. The method of claim 45, wherein the polymer carrier is poly(1-glutamic acid).
- 50. The method of claim 45, wherein the therapeutic agent is a chemotherapeutic agent.
- 51. The method of claim 45, wherein the administering step comprises intravascular, intraperitoneal or intramuscular injection.
- 52. The method of claim 45, wherein the patient is a mammal.
- 53. The method of claim 45, wherein the patient is a human.
- 54. The method of claim 45, wherein the diseased tissue is selected from the group consisting of a tumor, an inflammatory tissue, an infectious tissue, a reparative tissue and a regenerative tissue.
- 55. The method of claim 45, wherein the diseased tissue is a tumor
- 56. The method of claim 55, wherein the tumor is a solid tumor.
- 57. The method of claim 55, wherein the tumor is breast cancer, ovarian cancer, colon cancer, lung cancer, head and neck cancer, brain cancer, liver cancer, pancreatic cancer, bone cancer, prostate cancer, lymphoma or leukemia.
- 58. A method for synthesizing a conjugate molecule comprising the steps of:
providing a polymer spacer-polymer carrier construct having a sulfhydryl-reactive vinyl sulfone group at an end of the polymer spacer; conjugating the therapeutic agent to the polymer carrier to form a vinyl sulfone-polymer spacer-polymer carrier-therapeutic agent construct; pretreating the ligand to introduce sulfhydryl groups on the ligand; and combining the pretreated ligand with the vinyl sulfone-polymer spacer-polymer carrier-therapeutic agent construct, wherein the vinyl sulfone group reacts with the sulfhydryl group to form said conjugate molecule comprising said ligand, said polymer spacer, said polymer carrier, and said therapeutic agent, and wherein the ligand is bonded to the polymer spacer, the polymer spacer is bonded to the polymer carrier, and the polymer carrier is bonded to the therapeutic agent.
- 59. A method for synthesizing a conjugate molecule comprising:
introducing a protected sulfhydryl group (SH) to an end of a polymer spacer; conjugating the polymer spacer to a polymer carrier to form a protected SH-polymer spacer-polymer carrier construct; conjugating a therapeutic agent to the polymer carrier to form a protected SH-polymer spacer-polymer carrier-therapeutic agent construct; pretreating a ligand to introduce a sulfhydryl reactive functional group on said ligand; deprotecting the protected SH group to obtain a free SH group; and combining the pretreated ligand with the SH-polymer spacer-polymer carrier-therapeutic agent construct, wherein the SH group reacts with the sulfhydryl reactive functional group to form a conjugate molecule comprising the ligand, the polymer spacer, the polymer carrier, and the therapeutic agent, wherein the ligand is bonded to the polymer spacer, the polymer spacer is bonded to the polymer carrier, and the polymer carrier is bonded to the therapeutic agent.
- 60. The method of claim 59 wherein the ligand is pretreated with vinyl sulfone or maleimide to introduce the sulfhydryl reactive functional group.
- 61. A method for synthesizing a conjugate molecule comprising:
providing a polymer-spacer-polymer carrier-therapeutic agent construct; introducing a protected amine to an end of the polymer spacer to form a protected amine-polymer spacer-polymer carrier-therapeutic agent construct; deprotecting the protected amine-polymer spacer-polymer carrier-therapeutic agent construct to obtain a free amine-polymer spacer-polymer carrier-therapeutic agent construct; and combining the free amine-polymer spacer-polymer carrier-therapeutic agent construct with a ligand having a carboxylic acid group, wherein the carboxylic acid in the ligand conjugates with the free amine to form an amide bond, thereby forming a conjugate molecule comprising the ligand, the polymer spacer, the polymer carrier and the therapeutic agent, wherein the ligand is bonded to the polymer spacer, the polymer spacer is bonded to the polymer carrier, and the polymer carrier is bonded to the therapeutic agent.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of: U.S. Provisional Patent Application No. 60/286,453, entitled “Methods for Visualizing Tumors Using a Radioisotope Conjugate” filed Apr. 26, 2001; U.S. Provisional Patent Application No. 60/334,969, entitled “Therapeutic Agent/Ligand Conjugate Compositions and Methods of Use” filed Dec. 4, 2001; and U.S. Provisional Patent Application No. 60/343,147, entitled “Diagnostic Imaging Compositions, Their Methods of Synthesis and Use” filed Dec. 20, 2001, all three of which are hereby incorporated herein by reference in their entirety. This application is related to U.S. patent application Ser. No. , entitled “Diagnostic Imaging Compositions, Their Methods of Synthesis and Use,” filed Apr. 19, 2002, inventors Chun Li, et al., which is hereby incorporated herein by reference in its entirety.
RIGHTS IN THE INVENTION
[0002] This invention was made, in part, with United States Government support under grant CA 74819 from the NCI, and the United States Government may therefore have certain rights in the invention.
Provisional Applications (3)
|
Number |
Date |
Country |
|
60286453 |
Apr 2001 |
US |
|
60334969 |
Dec 2001 |
US |
|
60343147 |
Dec 2001 |
US |