Claims
- 1. An isolated leukocyte adhesion inhibitor-1 (LAI-1) polypeptide having an amino acid sequence selected from the group consisting of:
(a) Val (23)-Pro (109) of SEQ ID NO:4; (b) Val (26)-Pro (109) of SEQ ID NO:4; (c) Thr (29)-Pro (109) of SEQ ID NO:4; (d) Ser (30)-Pro (109) of SEQ ID NO:4; (e) a polypeptide having an amino acid sequence identical to that of (a) except for at least one amino acid substitution; (f) a polypeptide having an amino acid sequence identical to that of (b) except for at least one amino acid substitution; (g) a polypeptide having an amino acid sequence identical to that of (c) except for at least one amino acid substitution; and (h) a polypeptide having an amino acid sequence identical to that of (d) except for at least one amino acid substitution.
- 2. The isolated polypeptide of claim 1, which is produced recombinantly using a baculovirus expression system.
- 3. A method of inhibiting angiogenesis, comprising administering to an animal an effective amount of an LAI-1 polypeptide of claim 1.
- 4. A method of inhibiting adhesion of peripheral blood mononuclear cells (PBMCs) to endothelial cells comprising administering to an animal an effective amount of the polypeptide of claim 1.
- 5. The method of claim 4, wherein said inhibition alleviates or prevents joint inflammation.
- 6. An isolated Chemokine ,-11 polypeptide selected from the group consisting of:
(a) Gly (22)-Ser (98) of SEQ ID NO:2; and (b) a polypeptide having an amino acid sequence identical to that of (a) except for at least one amino acid substitution.
- 7. A method for attracting T-lymphocytes comprising administering to an animal an effective amount of the polypeptide of claim 6.
- 8. The isolated polypeptide of claim 1, wherein at position 30 of said amino acid sequence, Glu is substituted for Ser.
Parent Case Info
[0001] This application is a continuation-in-part of U.S. patent application Ser. No. 08/724,871 filed on Oct. 4, 1996, which is herein incorporated by reference. Further, this application claims the benefit of the filing date of U.S. provisional application No. 60/027,769 filed on Oct. 4, 1996, which is also herein incorporated by reference.
Provisional Applications (1)
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Number |
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60027769 |
Oct 1996 |
US |
Divisions (1)
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09635899 |
Aug 2000 |
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Child |
10247287 |
Sep 2002 |
US |
Continuations (2)
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08943336 |
Oct 1997 |
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Child |
09635899 |
Aug 2000 |
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Parent |
08724871 |
Oct 1996 |
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Child |
09635899 |
Aug 2000 |
US |
Continuation in Parts (4)
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PCT/US96/09572 |
Jun 1996 |
US |
Child |
08724871 |
Oct 1996 |
US |
Parent |
08460987 |
Jun 1995 |
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Child |
08724871 |
Oct 1996 |
US |
Parent |
08464401 |
Jun 1995 |
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Child |
08724871 |
Oct 1996 |
US |
Parent |
PCT/US95/01780 |
Feb 1995 |
US |
Child |
08724871 |
Oct 1996 |
US |