The invention relates to a method and a device for recording thermal images of a structure to be depicted and arranged under a sample surface, having a thermal imaging camera recording the sample surface, a source of electromagnetic radiation for illuminating the structure to be depicted and an evaluation unit for evaluating the surface measurement data recorded by the thermal imaging camera.
The use of an infrared camera for recording thermal images enables non-contact and simultaneous temperature measurement of many surface pixels. From these surface measurement data, a structure embedded in a sample, tissue or the like below a surface can be reconstructed and displayed when heated by an excitation pulse. The main disadvantage in the active thermography image is the loss of spatial resolution proportional to the depth below the sample surface. This results in blurred images for deeper structures.
For many imaging techniques, the possible spatial resolution is limited by the width of the point spread function (PSF), i.e. the image of a small object, ideally a point. In acoustics this corresponds to the diffraction limit or in optics to the Abbe limit. Both limits are proportional to the acoustic or optical wavelength. For smaller structures either higher spatial frequencies corresponding to shorter wavelengths, e.g. electrons, or near-field effects can be used. This is often not possible for biomedical and non-destructive imaging because the structures are embedded in a sample or tissue. Therefore, they are not suitable for near-field methods. Higher frequencies are attenuated below the noise level before they can be detected on the surface. Other high-resolution methods are necessary for the representation of such structures.
In their “Theory of High Resolution” Donoho et al. (D. L. Donoho, A. M. Johnstone, J. C. Hoche, and A. S. Stern, J. R. Statist. Soc. B 54, 41 (1992)) showed that high-resolution imaging can overcome such a resolution limit. When the noise is close to zero, the reconstructed image converges to the original object. For diffraction-limited imaging, they showed that nonlinear algorithms that obey a positivity constraint can obtain a high resolution. Already in 1972 Frieden (B. R. Frieden, J. Opt. Soc. Am. 62, 1202 (1972)) showed for a simulated object consisting of two narrow lines, which could not be resolved with a regression calculation according to the principle of the smallest squares, that his nonlinear reconstruction algorithm can resolve and represent the object.
In 1999, five years after its theoretical description, the first high-resolution far-field fluorescence microscopy was realized experimentally with STED microscopy (T. A. Klar and S. W. Hell, Opt. Lett. 24, 954 (1999)). Later, further high-resolution methods such as STORM, PALM or SOFI were developed, all of which exploit the fact that localization of point sources (e.g. activated fluorescent molecules) is possible with a higher accuracy than the width of the PSF.
The structured illumination microscopy (SIM—M. G. Gustafsson, J. Microscopy 198, 82 (2000)) uses several structured patterns as illumination for high-resolution imaging. The physical origin of the resolution increase is a frequency mixture between the frequencies of the illumination and the object frequencies. The high spatial frequencies in the object are transformed by this frequency mixing into the low frequency range given by the Fourier transform of the PSF and can therefore be depicted. Normally, reconstruction algorithms use the knowledge of the illumination patterns of the structured illumination to calculate the images. However, even small errors in the patterns can lead to errors in the final images. Therefore, a blind SIM was proposed where knowledge of the illumination pattern is not necessary. It is assumed that the illumination patterns are positive and their sum is homogeneous (E. Mudry, K. Belkebir, J. Girard, J. Savatier, E. L. Moal, C. Nicoletti, M. Allain, and A. Sentenac, Nat. Photon. 6, 312 (2012)), or additional restrictions such as the same absorption patterns for all illuminations, thin occupation of the functions or requirements for the covariance of the patterns are applied. Recently, two reconstruction algorithms have been proposed using thin occupation and equality of absorption patterns (so-called block sparsity), which have been successfully applied for acoustic resolution in photoacoustic microscopy. The spatial resolution limit given by the acoustic PSF could thus be largely improved by using illumination with unknown granular laser patterns (“speckle patterns”). The reconstruction algorithms used are also valuable for other imaging techniques where diffuse processes confuse high frequency structural information.
Thermographic imaging uses the pure diffusion of heat, sometimes referred to as thermal waves, wherein the structural information of thermal images is much more attenuated at higher image depths than by acoustic attenuation. Thermographic imaging has some advantages over other imaging techniques, e.g. ultrasound imaging. No coupling media such as water are required, and the temperature development of many surface pixels can be measured in parallel and without contact with an infrared camera. The main disadvantage of thermographic imaging is the sharp decrease in spatial resolution proportional to depth, resulting in blurred images for deeper structures.
It is the object of the invention to create a method and an associated device for the recording of thermal images which, compared to the prior art, enable a noticeably improved depth resolution with thermal images of measured structures. In particular, structures lying deeper under a surface should also be able to be displayed in a better way.
The invention solves this object with the features of the independent claim 1. Advantageous further developments of the invention are shown in the subclaims.
The invention overcomes the disadvantage, namely the loss of spatial resolution proportional to the depth below the sample surface, and enables higher resolution even for deeper lying structures by using (unknown) structured illumination and a non-linear iterative evaluation algorithm, which reduces the thin occupation (“sparsity”) and the constant location of the heated structures for the various structured illumination patterns (IJOSP algorithm—T. W. Murray, M. Haltmeier, T. Berer, E. Leiss-Holzinger, and P. Burgholzer, Optica 4, 17 (2017).
The unknown structured illumination can be light falling through moving slot diaphragms, as shown in the following example. When coherent light (laser, microwave or the like) is used, dark and bright spots, called laser speckles, are automatically produced in a scattering sample, such as a biological tissue, by interference phenomena, so that the use of a separate diaphragm can be dispensed with if necessary. These speckle patterns are used as unknown structured illumination and the size of the bright areas (speckles) depends on the light wavelength of the laser, the scattering properties of the sample and the penetration depth of the light in the sample.
According to the invention, the effect of the resolution decreasing proportionally with depth can be avoided if a known or unknown structured illumination and a nonlinear reconstruction algorithm are used to reconstruct the embedded structure. This makes it possible, for example, to depict line patterns or star-shaped structures through a 3 mm thick steel sheet with a resolution that is at least significantly better than the width of the thermographic point spread function (PSF). Further details are given in the embodiment example.
According to the invention, in order to avoid the disadvantage of the strong decrease of spatial resolution proportional to the depth of a sample under the sample surface, an unknown structured illumination is used together with an iterative algorithm, which exploits the thin occupation of the structures. The reason for this decrease in resolution with increasing depth is the entropy production during the diffusion of heat, which for macroscopic samples is equal to the loss of information and therefore limits the spatial resolution. The mechanism for the loss of information is thermodynamic fluctuation, which is extremely small for macroscopic samples. However, these fluctuations are highly amplified during the reconstruction of structural information from thermographic data (“badly positioned” inverse problem). The entropy production, which depends only on the mean temperature values, is for macroscopic samples equal to the loss of information caused by these fluctuations. For real heat diffusion processes these fluctuations cannot be described by simple stochastic processes, but for macroscopic samples the information loss depends only on the amplitude of the fluctuations in relation to the mean temperature signals, which corresponds to the signal-to-noise ratio (SNR). With this knowledge it is possible to derive a PSF from the SNR without calculating the information loss and entropy production.
In particular, the thermographic reconstruction is carried out in a three-stage process. In a first step, the measured time-dependent temperature signals Ts(r, t) are converted into a virtual acoustic signal as a function of location r and time t (see P. Burgholzer, M. Thor, J. Gruber, and G. Mayr, J. Appl. Phys. 121, 105102 (2017)). In a second step, an ultrasonic reconstruction procedure (e.g. FSAFT) is used to reconstruct y(r) as a space function. In a third step, the space-only IJOSP algorithm, a non-linear iterative algorithm, is used for thermographic reconstruction (T. W. Murray, M. Haltmeier, T. Berer, E. Leiss-Holzinger, and P. Burgholzer, Optica 4, 17 (2017)).
Only as a result of the spatially structured excitation, which is unknown, but statistically changes the measured signals significantly in several measurements, a “super-resolution” spatial resolution can be achieved by the used IJOSP algorithm. Super resolution is the name of this resolution because, analogous to optics, it enables a spatial resolution better than the wavelength (Abbe limit in optics), in this case the wavelength of the so-called “thermal wave”.
In the drawing and in the following embodiment example, the invention is shown by way of example, wherein:
Structure S is applied to the back of a 3 mm steel plate. In
In order to derive the thermographic PSF, the damping of a one-dimensional thermal wave is treated first.
T(z, t)=Real(T0ei(σz−ωt)), (1
where T(z,t) is the temperature as a function of the depth z of the sample and the time t, T0 is a complex constant to satisfy the boundary condition at the surface with z=0, σ is the complex wave number and w=2πf corresponds to the thermal wave frequency.
This solves the heat diffusion equation
where ∇2 is the Laplace operator, i.e. the second derivative in space, α is the material-dependent thermal diffusion coefficient assumed to be homogeneous in the sample, and μ≡√{square root over (2α/ω)} is defined as a thermal diffusion length where the amplitude of the thermal wave is reduced by a factor of 1/e. This results in eq. (1) as follows:
which describes an exponentially damped wave in z with the wave number or spatial frequency k≡1/μ. The cut-off wave number kcut, at which the signal for a depth z=a is attenuated to the noise level, results from equation (3) to form:
A higher spatial frequency than kcut cannot be resolved, since the signal amplitude falls below the noise level at a distance a. The same result can be derived for one-dimensional heat diffusion by setting the information loss equal to the mean entropy production. In order to obtain a two- or three-dimensional thermographic PSF, a point source is embedded in a homogeneous sample at a depth d related to a flat measuring surface. The distance a to the surface depends on the angle θ (
For a selected test arrangement (see
The lateral resolution of this PSF is used in the following for deconvolution or for the IJOSP reconstruction algorithm, which enables high resolution. The same PSF can be reconstructed from a point source using a two-step image reconstruction method. First, the measured signal is converted into virtual acoustic waves (see P. Burgholzer, M. Thor, J. Gruber, and G. Mayr, J. Appl. Phys. 121, 105102 (2017)), according to which any available ultrasonic reconstruction technique, such as the synthetic aperture focusing technique (F-SAFT), is used for the reconstruction. This method only produces a meaningful PSF if the measurement time is sufficient to measure the signals up to θ≈45° and use them for reconstruction. For shorter measuring times, only a small cone of the PSF in the Fourier space has the value one in the axial direction and the rest has the value zero. In real space, the axial resolution remains almost constant for shorter measurement times, while the lateral resolution becomes worse.
An experimental setup to illustrate this method according to the invention for high-resolution thermographic imaging comprises the following. A 3 mm thick steel sheet (standard structural steel with a thermal diffusivity of 16 mm2s−1) was blackened on both sides for improved heat absorption and dissipation. An absorbent pattern, such as parallel lines or a star, was created on the back of the steel sheet using an aluminum foil acting as a reflective mask. This ensures that only the unmasked (black) patterns absorb light from an optical flash arrangement irradiating this side (Blaesing P B G 6000 with 6 kJ electrical energy). An infrared camera (Ircam Equus 81k M Pro) was used to measure the temperature curve on the front side of the steel sheet. A three-dimensional thermographic imaging method is used for this purpose (P. Burgholzer, M. Thor, J. Gruber, and G. Mayr, J. Appl. Phys. 121, 105102 (2017)), whereby the image y (r) can be reconstructed as space function r of the absorbing pattern, wherein the folding of the absorbed light I(r) ρ(r) takes place with the thermographic PSF h (r) shown in
wherein ε (r) indicates the noise (error) in the data, ρ (r) indicates the optical absorption of the absorbing patterns, and I (r) is the illuminating luminous flux. The spatial variable r for the line pair patterns is described as a one-dimensional coordinate on the steel surface perpendicular to the lines (x-direction), and for two-dimensional patterns, such as a star, the two-dimensional Cartesian coordinate pair (x- and y-direction) is described on the back of the steel sheet.
In the first embodiment example (
y
m
=h*[Im·ρ]+ϵm for m=1, . . . , M (7)
The aim is to calculate the absorber distribution p and, to a certain extent, the illumination pattern Im from the data. The product Hm≡Im·ρ corresponds to the heat source assigned to the mth speckle pattern. The heat sources Hm are (theoretically) clearly determined by the deconvolution equations (7). However, due to the poorly conditioned deconvolution with a smooth core, these uncoupled equations are error-sensitive and only provide low-resolution reconstructions if they are solved independently and without appropriate regularization. In order to obtain high-resolution reconstructions, it is proposed according to the invention to use a reconstruction algorithm which takes advantage of the fact that all Hm come from the same density distribution ρ, which are also sparse, called IJOSP (iterative joint sparsity) algorithm.
Numerically this can be implemented by the following minimization
with the FISTA (Fast Iterative Threshold Algorithm—A. Beck and M. Teboulle “A Fast Iterative Shrinkage-Thresholding Algorithm for Linear Inverse Problems,” SIAM J. Imaging Sci. 2, 183-202 (2009)). The first term in equation (8) is the data adaptation term, the second term uses the thin occupation and equality of the density distribution ρ and the last term is a stability term known from the Tikhonov regulation for general inverse problems. As with other regulatory methods, α1 and α2 are regulatory parameters that must be adequately selected for the results presented in
∥H∥2,1≡Σi=1N√{square root over (Σm=1M|Hm(xi)|2)} (9)
provides solutions with minimization, which prefer thin occupation and equality of density distribution ρ. First, for each individual pixel measured, the l2-norm is taken over all M different illumination patterns and then these positive values are summed up (N pixels). This term favors blocked thin solutions, which means that it has a lower value for solutions that deviate from zero only in a few places, but becomes even lower if these entries are not equal to zero for all illumination patterns in the same place.
In the following, the measurement and reconstruction results for the four absorbing line pairs are presented.
In summary, the resolution for the line pairs could be improved from 6 mm lateral resolution (
For comparison, the line pattern p was calculated from equation (7) using the least squares method, taking into account known illumination patterns. The results for known illumination patterns were no better than the results for unknown patterns using IJOSP. In addition, three-dimensional high-resolution thermographic imaging is also possible using, for example, speckle patterns for illumination, in which the PSF is not evenly distributed over the region depicted, but increases with depth.
A light-scattering sample, for example biological tissue (
For the thermographic reconstruction, measured time-dependent temperature signals Ts(r, t), which use H(r, t), can also be used directly instead of the PSF h(r) from equation (6), whereby H then also includes the temporal temperature course of the heat diffusion.
Number | Date | Country | Kind |
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A50421/2017 | May 2017 | AT | national |
Filing Document | Filing Date | Country | Kind |
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PCT/AT2018/050007 | 5/2/2018 | WO | 00 |