TREA

TRANS-SPLICING SYSTEM FOR TISSUE-SPECIFIC REPLACEMENT OF RNA SEQUENCES

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Information

  • Patent Application
  • 20230340469
  • Publication Number
    20230340469
  • Date Filed
    March 24, 2023
    a year ago
  • Date Published
    October 26, 2023
    7 months ago
  • Inventors
    • Nelles; David A. (San Francisco, CA, US)
  • Original Assignees
    • Tacit Therapeutics, Inc. (South San Francisco, CA, US)
Information
Abstract
Disclosed are compositions comprising: a trans-splicing nucleic acid comprising (a) one or more replacement domains that encode a therapeutic sequence; and (b) one or more intronic domains that promote RNA splicing of the replacement domain primarily in a specific tissue or cell type; and (c) one or more antisense domains that promote binding to a target RNA molecule, wherein an RNA trans-splicing reaction promotes insertion of the replacement domain into a target RNA. Methods of making and methods of using compositions of the disclosure are also provided, including but not limited to compositions of the disclosure that may be used in the treatment of a disease or disorder in a patient or subject. Example disease or disorders of the disclosure include genetic and epigenetic diseases or disorders.
Description
SEQUENCE LISTING

The instant application contains a Sequence Listing which has been submitted electronically in XML format is hereby incorporated by reference in its entirety. Said XML copy, created on Jul. 18, 2023, is named 63827_702_301_SL.xml and is 1,655,569 bytes in size.


FIELD OF THE DISCLOSURE

The is a disclosure directed to gene therapy, molecular biology, and compositions and methods for altering the sequence composition of RNA molecules.


BACKGROUND

There is a long-felt but unmet need in the art for, 1) controlling the location and timing of gene therapy activity in patients, and 2) replacing disease-causing RNA sequences. The disclosure provides compositions and methods for restricting the activity of an RNA trans-splicing system to specific and chosen tissues. Specifically, the disclosures provides compositions and methods for insertion of chosen RNA sequences into target RNAs using trans-splicing mediated by introns that induce cell type-specific RNA splicing patterns.


Current approaches to achieve tissue- or cell type-specific activity of gene therapies rely on the use of tissue-restricted promoters. But an ideal therapeutic would involve the high expression levels associated with non-specific promoters while maintaining tissue specificity and extend the range of tissue-restricted systems beyond those available using tissue-restricted promoters. Alternative exon choice during RNA splicing follows highly tissue-restricted patterns.


SUMMARY

This disclosure provides a composition comprising a nucleic acid sequence encoding a trans-splicing RNA molecule comprising (a) at least one domain that promotes trans-splicing in a tissue-restricted manner (“Intronic Domain”), (b) at least one binding domain (“Antisense Domain”) that consists of a sequence complementary to a pre-mRNA present in a human cells (“Target RNA”), and (c) one coding domain that is inserted into the Target RNA via trans-splicing (“Replacement Domain”).


In some embodiments, the Antisense Domain is complementary to a gene (corresponding disease) that can cause disease in the brain and is selected from the group consisting of: SOD1, TARDBP, FUS, MATR3, SOD1, C9ORF72 (Amyotrophic lateral sclerosis); MAPT, PGRN (Frontotemporal dementia with parkinsonism); CDH23, MYO7A, USH2A (Usher's syndrome); GALC (Krabbe disease); SMPD1, NPC1, NPC2 (Niemann Pick disease); PRNP (prion disease); SCN1A (Dravet syndrome); PINK1, ATPGAP2 (early-onset Parkinson's disease); ATXN1, ATXN2, ATXN3, PLEKHG4, SPTBN2, CACNA1A, ATXN7, TTBK2, PPP2R2B, KCNC3, PRKCG, ITRP1, TBP, KCND1, FGF14 (spinocerebellar ataxics); SCN1A, SCN2A, CACNA1A, GRIN2B, GRIN2A, MECP2, FOXG1, SLC6A1, PRRT2, PTEN, KCNQ2, KCNQ3, STARD7, CLRN1 (genetic epilepsy disorders); ATM (Ataxia-telangiectasia); GLB1 (GM1 gangliosidosis); GBA (Gaucher disease); GM2A (GM2 gangliosidosis); UBE3A (Angelman syndrome); SLC2A1 (glucose transporter deficiency type 1); GLA (Fabry disease); MYOC, OPTN, TBK1, WDR36, CYPIB1 (Open Angle Glaucoma); IDUA (Hurler syndrome or Mucopolysaccharidosis 1); IDS (Hunter syndrome or Mucopolysaccharidosis 2); and CLN3 (Batten disease).


In some embodiments, the Intronic Domains promote trans-splicing primarily in brain tissue and are derived or isolated from a gene selected from the group consisting of: UBB, GFAP, STMN1, RPL13A, UCHL1, TUBB2B, ALDOA, LRRC75A-AS1, DYNLRB1, TPI1, PKM, NDRG2, MT3, RTN3, SKP1, RTN1, NRGN, DBNDD2, BSCL2, ALDOC, S100B, GNG3, FXYD6, PRDX1, HSP90AA1, SRP14, ATF4, NDUFB8, CAPZB, ATF5, ADD1, ERGIC3, GUK1, DCTN1, GSN, MORF4L1, GNAI2, SPP1, CRIP2, RNASE1, EIF4G2, PAQR6, DCTN3, CALB2, COMMD6, MEST, STXBP1, CLDND1, SH3GLB2, NREP, ABCA2, SUN2, UBXN6, DBI, CDK2AP1, NFIX, SF1, HNRNPF, GPM6B, SQSTM1, MOBP, ANAPC11, POLD2, SAMD4B, IST1, TPD52, SHD, STK25, ABR, PQBP1, PHB, SEPT9, S100A13, APBB1, RANBP1, ARHGEF2, DLGAP4, GNAO1, SEZ6L2, FDFT1, CBX1, EIF4G1, FAM213A, MAPK8IP1, PSME1, CCAR2, TKT, SYN1, OGDH, OCIAD1, SLC3A2, SLC1A5, PTTG1, DNM1, MAP6, KDM1A, PTN, FN1, CSNK1E, TMEM9, MATR3, AHCYL1, ATP6V0A1, CDIP1, CYFIP2, SLIRP, SCG5, TPX2, DMTN, SSBP1, PFKL, KIAA0930, CAMTA1, PRC1, CHTF8, TMPRSS3, BIN1, CCT8, AMZ2, TM7SF2, SEC11A, CLPTM1, AMPD2, DHX9, GAB2, CLTC, PSME3, RPP21, DYNC1I2, CDC20, NOSIP, ATP13A2, AIF1L, SURF4, PTPRN2, STK24, SDR39U1, HMOX2, UBAP2L, NRCAM, CCDC3, GPT2, TNPO2, NRXN2, PPP1R7, CAMK2A, UBE2L6, DBNL, ADAR, RNF220, MCM2, DTNA, PDIA6, PSMG2, URM1, SNX17, SV2A, SPG21, REEP2, HSD17B4, GSTO1, TRAPPC3, ZNF385A, SHISA5, FAM107B, MID1IP1, SERPINE2, CDK5RAP3, COMMD4, ANAPC15, CABP1, PTPRA, NPTN, RERE, VPS29, SPTBN2, MT1G, DTX3, GOLGA7, TPD52L2, EPB41L3, SPIN1, FARP1, NUP62, CNOT1, TMEM108, DUT, MAPRE2, PSD, NEBL, UBE2L3, KIF5C, TCF19, WDR54, CPSF7, GIT1, L1CAM, ITPR3, RHPN2, HMGA1, KCNAB2, HID1, SHF, CD99L2, C19ORF25, PAF1, B4GALT2, PTGES2, CAPG, SEMA4D, TTYH2, EXOC7, CNDP1, TACC2, BACE1, GABBR1, LMNB1, SLAIN1, GLG1, APBA2, CADM3, PC, PHLDB1, HHATL, SOGA1, CNTNAP2, RFC2, MAPK8IP3, CD200, REC8, TACC3, SYT13, SRP68, KLC4, CORO1C, ATPAF1, NARF, AP3B2, NUP93, FAM102A, SCN1B, NDUFS2, CBFA2T2, PSMA5, EPB41, and BCAR1.


In some embodiments, the Antisense Domain is complementary to a gene that can cause disease in the liver (corresponding disease) and is selected from the group consisting of: GLB1 (GM1 gangliosidosis); GBA (Gaucher disease); GM2A (GM2 gangliosidosis); PCSK9, LDLR, APOB, APOE (Familial hypercholesterolemia); GAA (Pompe disease); MYOC, OPTN, TBK1, WDR36, CYPIB1 (Open Angle Glaucoma); IDS (Hunter syndrome or Mucopolysaccharidosis 2); IDUA (Hurler syndrome or Mucopolysaccharidosis 1); CLN3 (Batten disease); F9 (Hemophilia B); and F8 (Hemophilia A).


In some embodiments, the Intronic Domains promote trans-splicing primarily in liver tissue and are derived or isolated from a gene selected from the group consisting of: ORM1, ORM2, PLG, ALDOB, CYP2C8, CPS1, ADH4, PON1, CFHR3, C6, DBI, ITIH3, ITIH4, C4BPB, PTGR1, C5, LBP, PRDX1, HPD, UBB, HPR, ADH6, RPL15, CXCL2, DPYS, HAL, SLC39A14, AQP9, SERPINA10, ADH1A, HABP2, SERPINF2, ECI2, APOM, KLKB1, ACSM5, SLCO1B3, ASGR2, IL1RAP, PSMA1, GRB14, LRRC75A-AS1, BCHE, EPHX1, DMGDH, MST1, ACSM3, SEPT2, TFR2, NDUFC1, NR1H4, AFMID, HNMT, TTC39C, RDH16, GPAM, SLC13A5, SERF2, PICALM, PCSK6, TOM1L1, ANPEP, EBNA1BP2, RCL1, DEPDC7, BTG1, KRT8, ABCA6, GJB1, KMO, HSD17B13, LEAP2, PECR, LEPR, CTNND1, ENSA, MASP2, KYNU, ABCB4, DNASE1L3, ACSL5, UBE2V1, SPTBN1, RBPMS, RAB13, IL1R1, SERPINA5, FGGY, TMEM220, ACSS2, VNN1, CTAGE5, MS4A6A, MFSD2A, SKAP1, VDAC2, SLC22A7, PPP2R5C, SORBS2, CDV3, CSAD, TRPM8, TOMM5, SLCO2B1, MICU1, NCOR1, GSAP, CBWD5, ENPP1, SPATS2L, FKBP5, NR3C1, IGF2, IGF1, PLSCR4, MYLK, SLC27A5, ALDH5A1, TXLNGY, PSMB5, MOCOS, PET100, SGK1, AZ12, TJP2, FMO4, STEAP1, DMD, RNF14, RBM34, PABPC1L, ZCCHC9, ANO6, AGMAT, MASP1, IL17RB, MRPS27, MLIP, MON2, ETF1, SULT1B1, GBP7, PRKACA, PI4K2B, SP100, TMED6, NFU1, RMDN2, IP6K2, CCNB1IP1, PCBP1-AS1, SLC39A1, and PHF11


In some embodiments, the Antisense Domain is complementary to a gene that can cause disease in the heart (corresponding disease) and is selected from the group consisting of: LAMP2 (Danon disease); GLA (Fabry disease); SLC2A1 (glucose transporter deficiency type 1); UBE3A (Angelman syndrome); MYOC, OPTN, TBK1, WDR36, CYPIB1 (Open Angle Glaucoma); IDUA (Hurler syndrome or Mucopolysaccharidosis 1); IDS (Hunter syndrome or Mucopolysaccharidosis 2); CLN3 (Batten disease); LMNA (Limb-girdle muscular dystrophy type 1B); DMD (Duchenne muscular dystrophy); DYSF (Limb-girdle muscular dystrophy type 2B); SGCB (Limb-girdle muscular dystrophy type 2E); SGCG (Limb-girdle muscular dystrophy type 2C); SGCA (Limb-girdle muscular dystrophy type 2D); SGCD (Limb-girdle muscular dystrophy type 2F); DUX4, D4Z4 (Facioscapulohumeral muscular dystrophy); F9 (Hemophilia B); F8 (Hemophilia A).


In some embodiments, the Intronic Domains promote trans-splicing primarily in heart tissue and are derived or isolated from a gene selected from the group consisting of: TNNI3, TNNT2, MB, FHL2, CSRP3, FHL1, UBB, MYOM2, CD36, GSN, PDHA1, CRIP2, POPDC2, SORBS2, CPT1B, RYR2, ANXA11, ITGA7, SORBS1, NDUFC1, NEXN, MYH7B, ABLIM1, NEBL, CAST, NME2, CYB5R3, TRDN, TACC2, NDUFA5, MYL12B, FHOD3, CALCOCO2, NCAM1, GPD1L, SGCA, AQP1, AKAP1, ARL6IP4, LRRC39, TTN, TSC22D1, PPP1R12B, OBSL1, MYO15B, PKIG, HMOX2, CDK18, BTNL9, ITGB1BP2, SEPT2, CAV2, DTNA, C15orf41, ATP1A1, BCO2, MYL4, ADCY5, CLIC5, SLC8A1, RRAD, AKAP13, AK3, MYO1C, FGF12, LAMA5, ADAM15, WNK2, SUN1, KLHL7, RNF207, HIF3A, HSPG2, LPCAT4, ADH1B, ENY2, RGS3, FRMD3, CCNB1IP1, PHC2, STAB1, HAND2-AS1, PTPN3, PARVB, PKD1, SPEG, SYNE2, ZNF638, SEPT4, DAG1, SLC12A7, FBXO18, FAM107A, RBM17, MYPN, GRIP2, SCN5A, WFS1, RABGAP1L, SYNE1, HLA-DRB5, SNRPN, MEG3, RAPGEF3, SEC13, C1QTNF1, UCKL1, FRY, IP6K2, CUX1, ANK3, MYH14, ADCY4, C3orf18, MYLK3, PXN, TSPAN18, PDE1C, C6orf48, BCS1L, ATG4D, HRAS, TENM2, PLXNB1, N4BP2L2, TNXB, LTBP1, MAPK8IP3, COL1A1, GOAT, ACOT11, MTUS2, VPS45, and KCNIP2.


In some embodiments, the Antisense Domain is complementary to a gene that can cause disease in the retina (corresponding disease) and is selected from the group consisting of: USHA2A, RPGR, RP2, RHO, PRPF31, USH1F, PRPF3, PRPF6 (Retinitis pigmentosa)


In some embodiments, the Intronic Domains promote trans-splicing primarily in retinal tissue and are derived or isolated from a gene selected from the group consisting of: ADGRV1, AMPH, ATP1B2, BSG, CASK, CC2D2A, CEP290, CLTC, DMD, DOC2B, EFR3A, ERP44, FARP2, FN3KRP, GRAMD1B, GSS, HCN1, IFT140, IMPDH1, INTS13, KCTD5, KIF1B, KIF1B, KMT2D, MADD, MAN2A2, MARCH1, MSI2, NAP1L4, PPP3CC, PROM1, PTPRF, PTPRF, RIMS2, SLC17A7, SLC4A7, STXBP5, TTC8, UNC13B, UNC13B, WDR47, and YKT6.


In some embodiments, the Antisense Domain is complementary to a gene that can cause disease in muscle (corresponding disease) and is selected from the group consisting of: LAMP2 (Danon disease); GLA (Fabry disease); SLC2A1 (glucose transporter deficiency type 1); UBE3A (Angelman syndrome); MYOC, OPTN, TBK1, WDR36, CYPIB1 (Open Angle Glaucoma); IDUA (Hurler syndrome or Mucopolysaccharidosis 1); IDS (Hunter syndrome or Mucopolysaccharidosis 2); CLN3 (Batten disease); LMNA (Limb-girdle muscular dystrophy type 1B); DMD (Duchenne muscular dystrophy); DYSF (Limb-girdle muscular dystrophy type 2B); SGCB (Limb-girdle muscular dystrophy type 2E); SGCG (Limb-girdle muscular dystrophy type 2C); SGCA (Limb-girdle muscular dystrophy type 2D); SGCD (Limb-girdle muscular dystrophy type 2F); DUX4, D4Z4 (Facioscapulohumeral muscular dystrophy); F9 (Hemophilia B); and F8 (Hemophilia A)


In some embodiments, the Intronic Domains promote trans-splicing primarily in muscle tissue and are derived or isolated from a gene selected from the group consisting of: ALDOA, TNNT3, MYBPC1, ATP2A1, NEB, STAC3, CMYA5, MYH7, TNNI1, CA3, ASB5, PLIN2, TRDN, OBSCN, RYR1, RAP1A, SPARCL1, CSNK1A1, NPEPPS, AKR1C3, IDI2, MBNL1, FNDC5, TMOD4, ATP6V1F, DAP3, KPNB1, NOMO2, BEST3, HP1BP3, SEPT2, TIMM23, MYPN, DHRS7C, PALLD, POLR2J3, KANSL3, THOC7, MEF2C, NOMO1, YWHAZ, ZBTB16, SPAG9, CCZ1, MYLK2, ASAH1, GTF2I, CCPG1, SURF4, SMARCA2, CLTCL1, SLC6A6, CHRNB1, MAFG, COMMD6, SLC7A8, CDK11B, and DNASE1L1.


In some embodiments, the Antisense Domain is complementary to a gene (corresponding accession numbers in brackets, corresponding disease in parentheses) that encodes a Target RNA that sometimes carry disease-causing mutations and is selected from the group consisting of: TNFRSF13B [ENSG00000240505] (common variable immune deficiency); ADA, CECR1 [ENSG00000196839, ENSG00000093072] (Adenosine deaminase deficiency); IL2RG [ENSG00000147168] (X-linked severe combined immunodeficiency); HBB [ENSG00000244734] (Beta-thassalemia); HBA1, HBA2 [ENSG00000206172, ENSG00000188536] (alpha-thassalemia); U2AF1 [ENSG00000160201] (myelodysplastic syndrome); SOD1, TARDBP, FUS, MATR3, SOD1, C9ORF72 [ENSG00000142168, ENSG00000120948, ENSG00000089280, ENSG00000015479, ENSG00000142168, ENSG00000147894] (Amyotrophic lateral sclerosis); MAPT, PGRN [ENSG00000186868, ENSG00000030582] (Frontotemporal dementia with parkinsonism); CDH23, MYO7A, USH2A [ENSG00000107736, ENSG00000137474, ENSG00000042781] (Usher's syndrome); GALC [ENSG00000054983] (Krabbe disease); SMPD1, NPC1, NPC2 [ENSG00000166311, ENSG00000141458, ENSG00000119655] (Niemann Pick disease); PRNP [ENSG00000171867] (prion disease); SCN1A [ENSG00000144285] (Dravet syndrome); PINK1, ATPGAP2 [ENSG00000158828] (early-onset Parkinson's disease); ATXN1, ATXN2, ATXN3, PLEKHG4, SPTBN2, CACNA1A, ATXN7, TTBK2, PPP2R2B, KCNC3, PRKCG, ITPR1, TBP, KCND1, FGF14 [ENSG00000124788, ENSG00000204842, ENSG00000066427, ENSG00000196155, ENSG00000173898, ENSG00000141837, ENSG00000163635, ENSG00000128881, ENSG00000156475, ENSG00000131398, ENSG00000126583, ENSG00000150995, ENSG00000112592, ENSG00000102057, ENSG00000102466] (spinocerebellar ataxias); SCN1A, SCN2A, CACNA1A, GRIN2B, GRIN2A, MECP2, FOXG1, SLC6A1, PRRT2, PTEN, KCNQ2, KCNQ3, STARD7, CLRN1 [ENSG00000144285, ENSG00000136531, ENSG00000141837, ENSG00000273079, ENSG00000183454, ENSG00000169057, ENSG00000176165, ENSG00000157103, ENSG00000167371, ENSG00000171862, ENSG00000075043, ENSG00000184156, ENSG00000084090, ENSG00000163646] (genetic epilepsy disorders); ATM [ENSG00000149311] (Ataxia-telangiectasia); GLB1 [ENSG00000170266] (GM1 gangliosidosis); GBA [ENSG00000177628] (Gaucher disease); GM2A [ENSG00000196743] (GM2 gangliosidosis); UBE3A [ENSG00000114062] (Angelman syndrome); SLC2A1 [ENSG00000117394] (glucose transporter deficiency type 1); LAM P2 [ENSG00000005893] (Danon disease); GLA [ENSG00000102393] (Fabry disease); PKD1, PKD2 [ENSG00000008710, ENSG00000118762] (Autosomal dominant polycystic kidney disease); GAA [ENSG00000171298] (Pompe disease); PCSK9, LDLR, APOB, APOE [ENSG00000169174, ENSG00000130164, ENSG00000084674, ENSG00000130203] (Familial hypercholesterolemia); MYOC, OPTN, TBK1, WDR36, CYPIB1 [ENSG00000034971, ENSG00000123240, ENSG00000183735, ENSG00000134987, ENSG00000138061] (Open Angle Glaucoma); IDUA [ENSG00000127415] (Hurler syndrome or Mucopolysaccharidosis 1); IDS [ENSG00000010404] (Hunter syndrome or Mucopolysaccharidosis 2); CLN3 [ENSG00000188603] (Batten disease); DMD [ENSG00000198947] (Duchenne muscular dystrophy); LMNA [ENSG00000160789] (Limb-girdle muscular dystrophy type 1B); DYSF [ENSG00000135636] (Limb-girdle muscular dystrophy type 2B); SGCA [ENSG00000108823] (Limb-girdle muscular dystrophy type 2D); SGCB [ENSG00000163069] (Limb-girdle muscular dystrophy type 2E); SGCG [ENSG00000102683] (Limb-girdle muscular dystrophy type 2C); SGCD [ENSG00000170624] (Limb-girdle muscular dystrophy type 2F); DUX4 [ENSG00000260596] (Facioscapulohumeral muscular dystrophy); F9 [ENSG00000101981] (Hemophilia B); F8 [ENSG00000185010] (Hemophilia A); USHA2A, RPGR, RP2, RHO, PRPF31, USH1F, PRPF3, PRPF6 [ENSG00000156313, ENSG00000102218, ENSG00000163914, ENSG00000105618, ENSG00000150275, ENSG00000117360, ENSG00000101161] (Retinitis pigmentosa); CFTR [ENSG00000001626] (cystic fibrosis); GJB2, GJB6, STRC, DFNA1, WFS1 [ENSG00000165474, ENSG00000121742, ENSG00000242866, ENSG00000131504, ENSG00000109501] (autosomal dominant hearing impairment); POU3F3 [ENSG00000198914] (nonsyndromic hearing loss).


In some embodiments, the Replacement domain is derived or isolated from the target RNA.


In some embodiments, the Intronic Domains carry binding sites that a preferentially-targeted by RNA-binding proteins with disease-causing mutations. In some embodiments, the dissociation constant of these mutated RNA-binding proteins and the Intronic Domain is lower than the dissociation constant of the non-mutated RNA-binding protein and the Intronic Domain.


In some embodiments, the Intronic Domains carry binding sites for RNA-binding proteins that promote trans-splicing primarily in 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more tissues but not other tissues. In some embodiments, the RNA-binding protein binding sites in the Intronic Domain are targeted by RNA-binding proteins selected from the group consisting of: DDX39, DDX3X, DDX3Y, DDX41, DDX5, DHX16, DHX8, DHX9, INTS6, DDX48, HNRNPA0, HNRNPA1, HNRNPA2B1, HNRPA3, HNRPAB, HNRPC, HNRPDL, HNRPF, HNRPH1, HNRPH2, HNRPLL, HNRPM, HNRPUL1, PTBP1, PTBP2, RBMXL2, SYNCRIP, CLK3, CLK4, SRPK1, SRPK2, SRPK3, ARS2, BUB3, C7ORF30, CIRBP, CRNKL1, CSDA, DNAJC8, EEF1A1, EIF3S10, FMR1, GRSF1, GTL3, IGF2BP3, IK, ILF2, ILF3, MATR3, MBNL1, MBNL2, MBNL3, MOV10, MSI1, MSI2, MYEF2, NHP2L1, PABPC1, PCBP1, PCBP2, PCBP4, PPIE, PPIL2, PPIL2, PPM1G, PPWD1, PRPF6, PSEN1, RALY, RBM15, RBM9, RBMS1, RBMX, RDBP, ROD1, SSB, THOC3, THOC4, TNPO1, TOP1MT, ZFP207, EFTUD2, LSM1, LSM2, LSM3, LSM4, LSM7, LSM8, NA, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SNRPA, SNRPA1, SNRPB, SNRPC, SNRPD1, SNRPD2, SNRPG, SNRPN, TXNL4, U2AF1L1, U2AF1L2, USP39, WDR57, PRPF31, PRPF4, PRPF40A, RBM17, NA, A2BP1, ACIN1, ASCC3L1, BCAS2, BRUNOL4, BUD13, BUD31, CD2BP2, CDC40, CDC5L, CRKRS, CUGBP2, ELAVL1, ELAVL1, ELAVL1, ELAVL2, ELAVL3, ELAVL4, FUSIP1, HSPA1B, HSPAS, HSPA8, NCBP2, NONO, NOVA1, NUDT21, PLRG1, PRPF19, PSIP1, RBM8A, RBMS1, SMU1, SNW1, TCERG1, TIA1, TIAL1, TNRC4, WBP11, WTAP, XAB2, YBX1, SFRS1, SFRS10, SFRS16, SFRS3, SFRS4, and SFRS6


In some embodiments, the trans-splicing RNA further comprises a 5′ untranslated region. In some embodiments, the 5′ untranslated region increases the stability of the trans-splicing nucleic acid. In some embodiments, the 5′ untranslated region reduces the stability of the trans-splicing nucleic acid. In some embodiments, the 5′ untranslated region alters the localization of the trans-splicing nucleic acid. In some embodiments, the 5′ untranslated region alters the processing of the trans-splicing nucleic acid.


In some embodiments, the trans-splicing RNA further comprises a 3′ untranslated region. In some embodiments, the 3′ untranslated region increases the stability of the trans-splicing nucleic acid. In some embodiments, the 3′ untranslated region reduces the stability of the trans-splicing nucleic acid. In some embodiments, the 3′ untranslated region alters the localization of the trans-splicing nucleic acid. In some embodiments, the 3′ untranslated region alters the processing of the trans-splicing nucleic acid.


In some embodiments, the intronic domains promote trans-splicing of the trans-splicing nucleic acid in a spatially-restricted manner so that trans-splicing occurs primarily in one tissue selected from the group consisting of: muscle cells, cardiac muscle cells, neurons, kidney cells, liver cells, epithelial cells, striatal tissue, basal ganglia tissue, substantia nigra tissue, lung tissue, spinal cord tissue, cranial nerve tissue, optic nerve tissue, oligodendrocytes, astrocytes, ependymal cells, microglia, airway tissue, inner eye tissue, retinal tissue, cerebellar tissue, pineal gland, thymus gland, pituitary gland, thyroid gland, adrenal gland, apocrine gland, holocrine gland, merocrine gland, serous gland, mucous gland, sebaceous gland, naïve T cells, effector T cells, helper T cells, memory T cells, regulatory T cells, gamma-delta T cells, natural killer cells, B cells, dendrocytes and macrophages.


In some embodiments, the Intronic Domain promote trans-splicing primarily in 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or more tissues or cell types but not trans-spliced in other cell types or tissues.


In some embodiments, the Intronic Domains broadly promote trans-splicing in many tissues but do not promote trans-splicing in a tissue selected from the following group consisting of: skeletal muscle cells, cardiac muscle cells, neurons, kidney cells, liver cells, epithelial cells, striatal tissue, basal ganglia tissue, substantia nigra tissue, lung tissue, spinal cord tissue, cranial nerve tissue, optic nerve tissue, oligodendrocytes, astrocytes, ependymal cells, microglia, airway tissue, inner eye tissue, retinal tissue, cerebellar tissue, pineal gland, thymus gland, pituitary gland, thyroid gland, adrenal gland, apocrine gland, holocrine gland, merocrine gland, serous gland, mucous gland, sebaceous gland, naïve T cells, effector T cells, helper T cells, memory T cells, regulatory T cells, gamma-delta T cells, natural killer cells, B cells, dendrocytes and macrophages. In some embodiments, the Intronic Domains do not promote trans-splicing in 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or more tissues but promote trans-splicing in other cell types or tissues.


In some embodiments, the Replacement Domain is comprised of sequence derived or isolated from a human gene. In some embodiments of the compositions of the disclosure, the sequence comprising the Replacement Domain has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 87%, 90%, 95%, 97%, 99% or any percentage in between of identity with a human gene. In some embodiments, the Replacement Domain has 100% identity with a sequence derived or isolated from a human gene. In some embodiments, the Replacement Domain comprises or consists of 2 nucleotides, 5 nucleotides, 10 nucleotides, 20 nucleotides, 30 nucleotides, 40 nucleotides, 50 nucleotides, 60 nucleotides, 70 nucleotides, 80 nucleotides, 90 nucleotides, 100 nucleotides, 110 nucleotides, 120 nucleotides, 130 nucleotides, 140 nucleotides, 150 nucleotides, 160 nucleotides, 170 nucleotides, 180 nucleotides, 190 nucleotides, 200 nucleotides, 210 nucleotides, 220 nucleotides, 230 nucleotides, 240 nucleotides, 250 nucleotides, 260 nucleotides, 270 nucleotides, more than 270 nucleotides, or any number of nucleotides in between.


In some embodiments of the compositions of the disclosure, the sequence comprising the Antisense Domain has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 87%, 90%, 95%, 97%, 99% or any percentage in between of complementarity to the Target RNA sequence. In some embodiments, the Antisense Domain has 100% complementarity to the Target RNA sequence. In some embodiments, the Antisense Domain comprises or consists of 20 nucleotides, 30 nucleotides, 40 nucleotides, 50 nucleotides, 60 nucleotides, 70 nucleotides, 80 nucleotides, 90 nucleotides, 100 nucleotides, 110 nucleotides, 120 nucleotides, 130 nucleotides, 140 nucleotides, 150 nucleotides, 160 nucleotides, 170 nucleotides, 180 nucleotides, 190 nucleotides, 200 nucleotides, 210 nucleotides, 220 nucleotides, 230 nucleotides, 240 nucleotides, 250 nucleotides, 260 nucleotides, 270 nucleotides, more than 270 nucleotides, or any number of nucleotides in between the complementary to the Target RNA sequence.


In some embodiments of the compositions of the disclosure, the sequence comprising the trans-splicing RNA further comprises a sequence encoding a promoter capable of expressing the trans-splicing RNA in a eukaryotic cell.


In some embodiments of the compositions of the disclosure, the eukaryotic cell is an animal cell. In some embodiments, the animal cell is a mammalian cell. In some embodiments, the animal cell is a human cell.


In some embodiments of the compositions and methods of the disclosure, a vector of the disclosure is a viral vector. In some embodiments, the viral vector comprises a sequence isolated or derived from a retrovirus. In some embodiments, the viral vector comprises a sequence isolated or derived from a lentivirus. In some embodiments, the viral vector comprises a sequence isolated or derived from an adenovirus. In some embodiments, the viral vector comprises a sequence isolated or derived from an adeno-associated virus (AAV). In some embodiments, the viral vector is replication incompetent. In some embodiments, the viral vector is isolated or recombinant. In some embodiments, the viral vector is self-complementary.


In some embodiments of the compositions and methods of the disclosure, the viral vector comprises a sequence isolated or derived from an adeno-associated virus (AAV). In some embodiments, the viral vector comprises an inverted terminal repeat sequence or a capsid sequence that is isolated or derived from an AAV of serotype AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV11 or AAV12. In some embodiments, the viral vector is replication incompetent. In some embodiments, the viral vector is isolated or recombinant (rAAV). In some embodiments, the viral vector is self-complementary (scAAV).


In some embodiments of the compositions and methods of the disclosure, a vector of the disclosure is a non-viral vector. In some embodiments, the vector comprises or consists of a nanoparticle, a micelle, a liposome or lipoplex, a polymersome, a polyplex, an exosome or a dendrimer. In some embodiments, the vector is an expression vector or recombinant expression system. As used herein, the term “recombinant expression system” refers to a genetic construct for the expression of certain genetic material formed by recombination.


In some embodiments of the compositions and methods of the disclosure, an expression vector, viral vector or non-viral vector provided herein, includes without limitation, an expression control element. An “expression control element” as used herein refers to any sequence that regulates the expression of a coding sequence, such as a gene. Exemplary expression control elements include but are not limited to promoters, enhancers, microRNAs, post-transcriptional regulatory elements, polyadenylation signal sequences, 5′ or 3′ untranslated regions, and introns.


Expression control elements may be constitutive, inducible, repressible, or tissue-specific, for example. A “promoter” is a control sequence that is a region of a polynucleotide sequence at which initiation and rate of transcription are controlled. It may contain genetic elements at which regulatory proteins and molecules may bind such as RNA polymerase and other transcription factors. In some embodiments, expression control by a promoter is tissue-specific. Non-limiting exemplary promoters include CMV, CBA, CAG, Cbh, EF-1a, PGK, UBC, GUSB, UCOE, hAAT, TBG, Desmin, MCK, C5-12, NSE, Synapsin, PDGF, MecP2, CaMKII, mGluR2, NFL, NFH, n132, PPE, ENK, EAAT2, GFAP, MBP, H1 and U6 promoters. In some embodiments, the promoter is a sequence isolated or derived from a promoter capable of driving expression of a transfer RNA (tRNA). In some embodiments, the promoter is isolated or derived from an alanine tRNA promoter, an arginine tRNA promoter, an asparagine tRNA promoter, an aspartic acid tRNA promoter, a cysteine tRNA promoter, a glutamine tRNA promoter, a glutamic acid tRNA promoter, a glycine tRNA promoter, a histidine tRNA promoter, an isoleucine tRNA promoter, a leucine tRNA promoter, a lysine tRNA promoter, a methionine tRNA promoter, a phenylalanine tRNA promoter, a proline tRNA promoter, a serine tRNA promoter, a threonine tRNA promoter, a tryptophan tRNA promoter, a tyrosine tRNA promoter, or a valine tRNA promoter. In some embodiments, the promoter is isolated or derived from a valine tRNA promoter.


An “enhancer” is a region of DNA that can be bound by activating proteins to increase the likelihood or frequency of transcription. Non-limiting exemplary enhancers and post-transcriptional regulatory elements include the CMV enhancer and WPRE.


In some embodiments of the compositions and methods of the disclosure, an expression vector, viral vector or non-viral vector provided herein, includes without limitation, vector elements such as an IRES or 2A peptide sites for configuration of “multicistronic” or “polycistronic” or “bicistronic” or tricistronic” constructs, i.e., having double or triple or multiple coding areas or exons, and as such will have the capability to express from mRNA two or more proteins from a single construct. Multicistronic vectors simultaneously express two or more separate proteins from the same mRNA. The two strategies most widely used for constructing multicistronic configurations are through the use of an IRES or a 2A self-cleaving site. An “IRES” refers to an internal ribosome entry site or portion thereof of viral, prokaryotic, or eukaryotic origin which are used within polycistronic vector constructs. In some embodiments, an IRES is an RNA element that allows for translation initiation in a cap-independent manner. The term “self-cleaving peptides” or “sequences encoding self-cleaving peptides” or “2A self-cleaving site” refer to linking sequences which are used within vector constructs to incorporate sites to promote ribosomal skipping and thus to generate two polypeptides from a single promoter, such self-cleaving peptides include without limitation, T2A, and P2A peptides or sequences encoding the self-cleaving peptides.


In some embodiments, the vector is a viral vector. In some embodiments, the vector is an adenoviral vector, an adeno-associated viral (AAV) vector, or a lentiviral vector. In some embodiments, the vector is a retroviral vector, an adenoviral/retroviral chimera vector, a herpes simplex viral I or II vector, a parvoviral vector, a reticuloendotheliosis viral vector, a polioviral vector, a papillomaviral vector, a vaccinia viral vector, or any hybrid or chimeric vector incorporating favorable aspects of two or more viral vectors. In some embodiments, the vector further comprises one or more expression control elements operably linked to the polynucleotide. In some embodiments, the vector further comprises one or more selectable markers. In some embodiments, the AAV vector has low toxicity. In some embodiments, the AAV vector does not incorporate into the host genome, thereby having a low probability of causing insertional mutagenesis. In some embodiments, the AAV vector can encode a range of total polynucleotides from 0.3 kb to 4.75 kb. In some embodiments, exemplary AAV vectors that may be used in any of the herein described compositions, systems, methods, and kits can include an AAV1 vector, a modified AAV1 vector, an AAV2 vector, a modified AAV2 vector, an AAV3 vector, a modified AAV3 vector, an AAV4 vector, a modified AAV4 vector, an AAV5 vector, a modified AAV5 vector, an AAV6 vector, a modified AAV6 vector, an AAV7 vector, a modified AAV7 vector, an AAV8 vector, an AAV9 vector, an AAV.rh10 vector, a modified AAV.rh10 vector, an AAV.rh32/33 vector, a modified AAV.rh32/33 vector, an AAV.rh43 vector, a modified AAV.rh43 vector, an AAV.rh74 vector, a modified AAV.rh74 vector, an AAV.rh64R1 vector, and a modified AAV.rh64R1 vector and any combinations or equivalents thereof. In some embodiments, the lentiviral vector is an integrase-competent lentiviral vector (ICLV). In some embodiments, the lentiviral vector can refer to the transgene plasmid vector as well as the transgene plasmid vector in conjunction with related plasmids (e.g., a packaging plasmid, a rev expressing plasmid, an envelope plasmid) as well as a lentiviral-based particle capable of introducing exogenous nucleic acid into a cell through a viral or viral-like entry mechanism. Lentiviral vectors are well-known in the art (see, e.g., Trono D. (2002) Lentiviral vectors, New York: Spring-Verlag Berlin Heidelberg and Durand et al. (2011) Viruses 3(2):132-159 doi: 10.3390/v3020132). In some embodiments, exemplary lentiviral vectors that may be used in any of the herein described compositions, systems, methods, and kits can include a human immunodeficiency virus (HIV) 1 vector, a modified human immunodeficiency virus (HIV) 1 vector, a human immunodeficiency virus (HIV) 2 vector, a modified human immunodeficiency virus (HIV) 2 vector, a sooty mangabey simian immunodeficiency virus (SIVSM) vector, a modified sooty mangabey simian immunodeficiency virus (SIVSM) vector, a African green monkey simian immunodeficiency virus (SIVAGM) vector, a modified African green monkey simian immunodeficiency virus (SIVAGM) vector, an equine infectious anemia virus (EIAV) vector, a modified equine infectious anemia virus (EIAV) vector, a feline immunodeficiency virus (FIV) vector, a modified feline immunodeficiency virus (FIV) vector, a Visna/maedi virus (VNV/VMV) vector, a modified Visna/maedi virus (VNV/VMV) vector, a caprine arthritis-encephalitis virus (CAEV) vector, a modified caprine arthritis-encephalitis virus (CAEV) vector, a bovine immunodeficiency virus (BIV), or a modified bovine immunodeficiency virus (BIV).


In some embodiments, the trans-splicing nucleic acid is RNA, DNA, a DNA/RNA hybrid, and/or comprises at least one of a nucleic acid analog, a chemically-modified nucleic acid, or a chimera composed of two or more nucleic acids or nucleic acid analogs. As used herein, the term “nucleic acid analog” refers to a compound having structural similarity to a canonical purine or pyrimidine base occurring in DNA or RNA. The nucleic acid analog may contain a modified sugar and/or a modified nucleobase, as compared to a purine or pyrimidine base occurring naturally in DNA or RNA. In some embodiments, the nucleic acid analog is a 2′-deoxyribonucleoside, 2′-ribonucleoside, 2′-deoxyribonucleotide or a 2′-ribonucleotide, wherein the nucleobase includes a modified base (such as, for example, xanthine, uridine, oxanine (oxanosine), 7-methlguanosine, dihydrouridine, 5-methylcytidine, C3 spacer, 5-methyl dC, 5-hydroxybutynl-2′-deoxyuridine, 5-nitroindole, 5-methyl iso-deoxycytosine, iso deoxyguanosine, deoxyuradine, iso deoxycytidine, other 0-1 purine analogs, N-6-hydroxylaminopurine, nebularine, 7-deaza hypoxanthine, other 7-deazapurines, and 2-methyl purines). In some embodiments, the nucleic acid analog may be selected from the group consisting of inosine, 7-deaza-2′-deoxyinosine, 2′-aza-2′-deoxyinosine, PNA-inosine, morpholino-inosine, LNA-inosine, phosphoramidate-inosine, 2′-O-methoxyethyl-inosine, and 2′-OMe-inosine. In other embodiments the nucleic acid analog is a nucleic acid mimic (such as, for example, artificial nucleic acids and xeno nucleic acids (XNA).





BRIEF DESCRIPTION OF THE DRAWINGS


FIG. 1 is an illustration of the unmet need addressed by the present invention. FIG. 1A indicates that human disease can affect some tissue and not others, or can primarily occur in some tissues compared to others. FIG. 1B demonstrates that therapeutic activity in a patient typically occurs in not only affected tissues but also unaffected tissues. FIG. 1C demonstrates the present invention which limits the activity of a trans-splicing therapeutic to the target tissue and not others.



FIG. 2 describes an example embodiment of the present invention along with a schematic to illustrate the activity. FIG. 2A describes, in general, human disease caused by genetic mutations. FIG. 2B describes a example embodiment of the present invention that involves 2 antisense domains, 2 intronic domains, and one replacement domain. FIG. 3C describes the tissue-restricted activity of the present invention so that trans-splicing occurs only in the target tissue resulting in replacement of the defective RNA sequence (top box).



FIG. 3 is a non-limiting illustration of the origin and structure of intronic domains in some embodiments of the present invention. FIG. 3A illustrates intronic domains that are taken from a gene that experiences tissue-specific alternative splicing of an adjacent exon. FIG. 3B illustrates intronic domains that are composed of combinations of introns derived from genes that experience tissue-specific alternative splicing of adjacent alternative exons.



FIG. 3C illustrates intronic domains that are composed of combinations of sequences that comprise binding sites for RNA-binding proteins that promote tissue-specific splicing patterns.



FIG. 4 provides a schematic of an experiment that results in identification of intronic domains that promote tissue- or cell type-specific trans-splicing. FIG. 4A illustrates that two functional components of the experiment: a reporter that reveals tissue-specific trans-splicing activity and a trans-splicing RNA that carries the candidates intronic domains. The reporter carries a promoter and incomplete coding sequence for GFP (N-terminal and C-terminal portions of GFP or “N-GFP” and “C-GFP”) that requires the internal section of GFP from the trans-splicing reaction to form a complete and fluorescent GFP protein. The trans-splicing RNA carries this internal GFP section (“int-GFP”) that is flanked by intronic domains and antisense domains. When the reporter is transfected into a human cell, there is little or no GFP signal (FIG. 4B, top). When the reporter and trans-splicing RNA are co-transfected in a non-target cell type, there is similarly little or no GFP signal (FIG. 4B, middle). When the reporter and trans-splicing RNA are co-transfected in a target cell type, GFP signal is produced (FIG. 4B, bottom). In this manner, intronic domains that promote trans-splicing in target cell types or tissues but not others can be identified.



FIG. 5 provides a schematic of an experiment that results in identification of trans-splicing systems that reverse the molecular caused of disease LGMD1B specifically in muscle tissue. FIG. 5A describes the LGMD1B reporter that carries that characteristic mutation near exon 9 of gene LM NA that promotes inclusion of exon 9 and resulting premature termination of the coding sequence. To reverse this disease-causing mutation, the trans-splicing RNA (FIG. 5A, bottom) targets exon 9 and replaces it with a short replacement domain that does not contain a stop codon (FIG. 5C, bottom). In a non-target cell type, the system is not active (FIG. 5B, top). In this manner, a high-quality trans-splicing system that can efficiently replace this defective sequence in LMNA can be identified.





DETAILED DESCRIPTION

This disclosure provides a therapeutic combination of replacement of disease-causing RNA sequences and tissue-restricted therapeutic activity. Specifically, the disclosure provides compositions and methods for specifically targeting disease-causing RNA molecules in specific, chosen tissues and replacing disease-causing RNA sequences within these RNA molecules. In particular, the disclosure provides a composition comprising a nucleic acid sequence encoding a trans-splicing therapeutic systems comprising (a) at least one domain that promotes trans-splicing in a tissue-restricted manner, (b) at least one binding domain that consists of a sequence complementary to a pre-mRNA present in a human cells, and (c) one coding domain that is inserted into the Target RNA via trans-splicing, wherein the target RNA causes disease, and wherein the intronic domain promotes trans-splicing specifically in chosen tissue but not others, and wherein the replacement domain encodes a non-mutated sequence. This disclosure provides vectors, compositions and cells comprising the trans-splicing compositions and methods of using the trans-splicing compositions.


Intronic Domains

Compositions comprising intronic domains disclosed herein include any sequences that promote trans-splicing in a manner restricted to specific cell or tissue types or groups of cell or tissue types.


Compositions comprising intronic domains disclosed herein include any sequence that promotes trans-splicing primarily in brain tissue. In some embodiments, the intronic domains are derived or isolated from human genes that experience brain-specific alternative splicing events. Exemplary intronic domains that promote brain-specific trans-splicing include without limitation genes such as: UBB, GFAP, STMN1, RPL13A, UCHL1, TUBB2B, ALDOA, LRRC75A-AS1, DYNLRB1, TPI1, PKM, NDRG2, MT3, RTN3, SKP1, RTN1, NRGN, DBNDD2, BSCL2, ALDOC, S100B, GNG3, FXYD6, PRDX1, HSP90AA1, SRP14, ATF4, NDUFB8, CAPZB, ATF5, ADD1, ERGIC3, GUK1, DCTN1, GSN, MORF4L1, GNAI2, SPP1, CRIP2, RNASE1, EIF4G2, PAQR6, DCTN3, CALB2, COMMD6, MEST, STXBP1, CLDND1, SH3GLB2, NREP, ABCA2, SUN2, UBXN6, DBI, CDK2AP1, NFIX, SF1, HNRNPF, GPM6B, SQSTM1, MOBP, ANAPC11, POLD2, SAMD4B, IST1, TPD52, SHD, STK25, ABR, PQBP1, PHB, SEPT9, S100A13, APBB1, RANBP1, ARHGEF2, DLGAP4, GNAO1, SEZ6L2, FDFT1, CBX1, EIF4G1, FAM213A, MAPK8IP1, PSME1, CCAR2, TKT, SYN1, OGDH, OCIAD1, SLC3A2, SLC1A5, PTTG1, DNM1, MAP6, KDM1A, PTN, FN1, CSNK1E, TMEM9, MATR3, AHCYL1, ATP6V0A1, CDIP1, CYFIP2, SLIRP, SCG5, TPX2, DMTN, SSBP1, PFKL, KIAA0930, CAMTA1, PRC1, CHTF8, TMPRSS3, BIN1, CCT8, AMZ2, TM7SF2, SEC11A, CLPTM1, AMPD2, DHX9, GAB2, CLTC, PSME3, RPP21, DYNC1I2, CDC20, NOSIP, ATP13A2, AIF1L, SURF4, PTPRN2, STK24, SDR39U1, HMOX2, UBAP2L, NRCAM, CCDC3, GPT2, TNPO2, NRXN2, PPP1R7, CAMK2A, UBE2L6, DBNL, ADAR, RNF220, MCM2, DTNA, PDIA6, PSMG2, URM1, SNX17, SV2A, SPG21, REEP2, HSD17B4, GSTO1, TRAPPC3, ZNF385A, SHISA5, FAM107B, MID1IP1, SERPINE2, CDK5RAP3, COMMD4, ANAPC15, CABP1, PTPRA, NPTN, RERE, VPS29, SPTBN2, MT1G, DTX3, GOLGA7, TPD52L2, EPB41L3, SPIN1, FARP1, NUP62, CNOT1, TMEM108, DUT, MAPRE2, PSD, NEBL, UBE2L3, KIF5C, TCF19, WDR54, CPSF7, GIT1, L1CAM, ITPR3, RHPN2, HMGA1, KCNAB2, HID1, SHF, CD99L2, C19ORF25, PAF1, B4GALT2, PTGES2, CAPG, SEMA4D, TTYH2, EXOC7, CNDP1, TACC2, BACE1, GABBR1, LMNB1, SLAIN1, GLG1, APBA2, CADM3, PC, PHLDB1, HHATL, SOGA1, CNTNAP2, RFC2, MAPK8IP3, CD200, REC8, TACC3, SYT13, SRP68, KLC4, CORO1C, ATPAF1, NARF, AP3B2, NUP93, FAM102A, SCN1B, NDUFS2, CBFA2T2, PSMA5, EPB41, and BCAR1.


Compositions comprising intronic domains disclosed herein include any sequence that promotes trans-splicing primarily in liver tissue. In some embodiments, the intronic domains are derived or isolated from human genes that experience liver-specific alternative splicing events. Exemplary intronic domains that promote liver-specific trans-splicing include without limitation genes such as: ORM1, ORM2, PLG, ALDOB, CYP2C8, CPS1, ADH4, PON1, CFHR3, C6, DBI, ITIH3, ITIH4, C4BPB, PTGR1, C5, LBP, PRDX1, HPD, UBB, HPR, ADH6, RPL15, CXCL2, DPYS, HAL, SLC39A14, AQP9, SERPINA10, ADH1A, HABP2, SERPINF2, ECI2, APOM, KLKB1, ACSM5, SLCO1B3, ASGR2, IL1RAP, PSMA1, GRB14, LRRC75A-AS1, BCHE, EPHX1, DMGDH, MST1, ACSM3, SEPT2, TFR2, NDUFC1, NR1H4, AFMID, HNMT, TTC39C, RDH16, GPAM, SLC13A5, SERF2, PICALM, PCSK6, TOM1L1, ANPEP, EBNA1BP2, RCL1, DEPDC7, BTG1, KRT8, ABCA6, GJB1, KMO, HSD17B13, LEAP2, PECR, LEPR, CTNND1, ENSA, MASP2, KYNU, ABCB4, DNASE1L3, ACSL5, UBE2V1, SPTBN1, RBPMS, RAB13, IL1R1, SERPINA5, FGGY, TMEM220, ACSS2, VNN1, CTAGE5, MS4A6A, MFSD2A, SKAP1, VDAC2, SLC22A7, PPP2R5C, SORBS2, CDV3, CSAD, TRPM8, TOMM5, SLCO2B1, MICU1, NCOR1, GSAP, CBWD5, ENPP1, SPATS2L, FKBP5, NR3C1, IGF2, IGF1, PLSCR4, MYLK, SLC27A5, ALDH5A1, TXLNGY, PSMB5, MOCOS, PET100, SGK1, AZ12, TJP2, FMO4, STEAP1, DMD, RNF14, RBM34, PABPC1L, ZCCHC9, ANO6, AGMAT, MASP1, IL17RB, MRPS27, MLIP, MON2, ETF1, SULT1B1, GBP7, PRKACA, PI4K2B, SP100, TMED6, NFU1, RMDN2, IP6K2, CCNB1IP1, PCBP1-AS1, SLC39A1, and PHF11


Compositions comprising intronic domains disclosed herein include any sequence that promotes trans-splicing primarily in heart tissue. In some embodiments, the intronic domains are derived or isolated from human genes that experience heart-specific alternative splicing events. Exemplary intronic domains that promote heart-specific trans-splicing include without limitation genes such as: TNNI3, TNNT2, MB, FHL2, CSRP3, FHL1, UBB, MYOM2, CD36, GSN, PDHA1, CRIP2, POPDC2, SORBS2, CPT1B, RYR2, ANXA11, ITGA7, SORBS1, NDUFC1, NEXN, MYH7B, ABLIM1, NEBL, CAST, NME2, CYB5R3, TRDN, TACC2, NDUFA5, MYL12B, FHOD3, CALCOCO2, NCAM1, GPD1L, SGCA, AQP1, AKAP1, ARL6IP4, LRRC39, TTN, TSC22D1, PPP1R12B, OBSL1, MYO15B, PKIG, HMOX2, CDK18, BTNL9, ITGB1BP2, SEPT2, CAV2, DTNA, C15orf41, ATP1A1, BCO2, MYL4, ADCY5, CLIC5, SLC8A1, RRAD, AKAP13, AK3, MYO1C, FGF12, LAMA5, ADAM15, WNK2, SUN1, KLHL7, RNF207, HIF3A, HSPG2, LPCAT4, ADH1B, ENY2, RGS3, FRMD3, CCNB1IP1, PHC2, STAB1, HAND2-AS1, PTPN3, PARVB, PKD1, SPEG, SYNE2, ZNF638, SEPT4, DAG1, SLC12A7, FBXO18, FAM107A, RBM17, MYPN, GRIP2, SCN5A, WFS1, RABGAP1L, SYNE1, HLA-DRB5, SNRPN, MEG3, RAPGEF3, SEC13, C1QTNF1, UCKL1, FRY, IP6K2, CUX1, ANK3, MYH14, ADCY4, C3orf18, MYLK3, PXN, TSPAN18, PDE1C, C6orf48, BCS1L, ATG4D, HRAS, TENM2, PLXNB1, N4BP2L2, TNXB, LTBP1, MAPK8IP3, COL1A1, GCAT, ACOT11, MTUS2, VPS45, and KCNIP2.


Compositions comprising intronic domains disclosed herein include any sequence that promotes trans-splicing primarily in muscle tissue. In some embodiments, the intronic domains are derived or isolated from human genes that experience muscle-specific alternative splicing events. Exemplary intronic domains that promote muscle-specific trans-splicing include without limitation genes such as: ALDOA, TNNT3, MYBPC1, ATP2A1, NEB, STAC3, CMYA5, MYH7, TNNI1, CA3, ASB5, PLIN2, TRDN, OBSCN, RYR1, RAP1A, SPARCL1, CSNK1A1, NPEPPS, AKR1C3, IDI2, MBNL1, FNDC5, TMOD4, ATP6V1F, DAP3, KPNB1, NOMO2, BEST3, HP1BP3, SEPT2, TIMM23, MYPN, DHRS7C, PALLD, POLR2J3, KANSL3, THOC7, MEF2C, NOMO1, YWHAZ, ZBTB16, SPAG9, CCZ1, MYLK2, ASAH1, GTF2I, CCPG1, SURF4, SMARCA2, CLTCL1, SLC6A6, CHRNB1, MAFG, COMMD6, SLC7A8, CDK11B, and DNASE1L1.


Compositions comprising intronic domains disclosed herein include any sequence that promotes trans-splicing primarily in retina tissue. In some embodiments, the intronic domains are derived or isolated from human genes that experience retina-specific alternative splicing events. Exemplary intronic domains that promote retina-specific trans-splicing include without limitation genes such as: ADGRV1, AMPH, ATP1B2, BSG, CASK, CC2D2A, CEP290, CLTC, DMD, DOC2B, EFR3A, ERP44, FARP2, FN3KRP, GRAMD1B, GSS, HCN1, IFT140, IMPDH1, INTS13, KCTD5, KIF1B, KIF1B, KMT2D, MADD, MAN2A2, MARCH1, MSI2, NAP1L4, PPP3CC, PROM1, PTPRF, PTPRF, RIMS2, SLC17A7, SLC4A7, STXBP5, TTC8, UNC13B, UNC13B, WDR47, and YKT6.


Intronic Domains that Promote Trans-Splicing Primarily in Brain Tissue


In some embodiments of the compositions of the disclosure, there may be an intronic domain that promotes trans-splicing primarily in brain tissue.


In some embodiments of the compositions of the disclosure, the intronic domain is derived or isolated from human genes that feature exons that are primarily included in brain tissue during the process of RNA splicing.


In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PSMA5. In some embodiments, the sequences from gene PSMA5 comprise or consist of:











(SEQ ID NO: 1)



CGAAGGGACTCGTCCGCGGCGCCGGCTCAGACCCACTGTAGCCGA







CTCATTACCGCCCAAAGTCCTGGAGCCCTCGACCGGGCATGGAGA







ACACCGTGGAGGAACCCTAAGAGAGGGAATCCTGCGAAGAGAAGT







CAGGTGGTGGCCTTGCCGGAAACGGGACCAAAGTCCTCTAGCTGG







GATTACGGGAGCCGGCGAGGGACCTATTATGTCAGGGTTCAGACA







ACCCAGCTCTGCATGCAAGCCTTTCCAATTGCCAGTCTTGGGGCG







GGCTCACATCTTCTCTACAAGGCGAACTCACTACACGCCTTGGGT







GCCTATCGTCCCTAGCAACCACCCGCGATTTAAGGCGGAAGTACC







TCCCTGGTCCTCCTCCTCCTCCGGACCAACCAAGCCCTCGTGAGG







AAGCGCGTTGGCCCAATCACCTAGGCGGGAACTACTACCAGCCAA







TAGGAAGCAGGCACAGGAGGGCGGGCTAAAGAGGTCGCGGTCGCA







CGCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GUK1. In some embodiments, the sequences from gene GUK1 comprise or consist of:











(SEQ ID NO: 2)



TCAAGCGACCGTCCCACGTCAGCCTCCCGAGTAGCAAGGACTACA







GGCACAAAAAAGTTTTTAAATTTTTTGGTACAGAAGGGGTTTCCC







TATGATCCCCAGGCTGGTCTCCAATTCCTGGCCTCAAGCGGTCCT







CCCGGCTCGGACTCCCAAAGAGCTGGGATCACAGACGTGAACCAC







GCGCCCGGCCACATTCCCCAATTAATGGTTTTTGGAGAGTGGCCG







GTTTGGAACGGGCTAGCGGAGTCGTTCGTTAGTTAGGTCCCTGCA







GTGTAGATGGAGGACGGAAACGGCGCTGTGACATAAGCGGGAGGA







GGCGGGCTGAGGCAGCGCTGTGACGTAGCAGATGAAAGCCGGGCC







CGCGCTGCACTGAGGTACTTCTCTGAGTGACAAGCGGGACAAGTA







CGCCGTGGGCGGGGCCGGCGGGTCGTGATGTTAGTTCGCGCTGTG







TGGAGGCGGGCCGGCGTGGCGCTGTGACGCAGCGGGAGAAGACGG







GCTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene KCNAB2. In some embodiments, the sequences from gene KCNAB2 comprise or consist of:











(SEQ ID NO: 3)



GGCGCGGCCAGGCGGGGTCCGCGGACTCCGGCCTGGGAAGGATGC







GCGCGGACTAGGGCGCGGGGAGCCAAGGCCCAGAACATCGGTACC







GCGCTCCCTTCAGCCGAGGGGCTGCAGAGAGGCCAGGGGGCAGGG







GGCAGGGGGCAGGGGGCAGGGGGCAAAGGGCAAGGGCAGAAAGGA







ACCCACGTAGCCAAACGGCACCCAGCGGCCCCGACCCCAAGTGTC







CCGACGAGGGGGGACGCCTGCGACTAACGCGGGGACAGCCCCCGC







CGCGCGCCACCGCCGCACCCCAGGCACCGGGCCCCCACGCAGGGA







CGGCGTGGACCACCCTACCCCCGAACCCCGAGGCTCTGGCCAACA







CTGAGGCCCCCTGTGCCTCGAGGCCGCGTCCAACCTCCCCCGCGG







CGCCGGCCCACATGGTTCCGCCCGCCGCGGGCCGCGTTACCTAGG







AGACCGACCCGCCGCGGCCGCGCCGGAGGCCACGGAGCCCACGCT







TTTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene KCNAB2. In some embodiments, the sequences from gene KCNAB2 comprise or consist of:











(SEQ ID NO: 4)



TGAGTACCGGGGAGACCCCGGCGGGGCGCGCTTTCCACCAAGCTG







CTCCGGGACGGCGGCGGCGGGTATCCGCGAGGCCCGCGCTGCGGC







AGGCGCCGCGGTCCCGTCTCCATCTCTTCTTCCCCGGCCCTGCGT







CCCTGCCTCCGCCGCTGTCTCACCTCCTCTCCAGCCTCCTCACCT







CCTTCTCCATCGTCTCCCTTCCCCCATCCCTTTCTGTCTTTCCTT







CCCTGTCCTCCCGCCCGCCCGACCCTTCAGCTCGCATCCCTGACC







CCTGCCCCTGCCGCCGTCCCTCTTCCCGGTGCACCTCTTCCCCAT







CTCTGCCCGCTCCGGTCCTAGCAGCCTCGCCCTCCCCTTTCCTCC







ATCCTCCTCTCATCCCCTCTCCCGGCCTCTGCGCAGCCCCACCGG







GGCGCTGCTGTTCCCGGTCCTCCCCATCTCTCCTCCTGATCGCTT







CTCAAGGCTCATCCGTCGTTCTCGCTGCCTGTCCCTGTGTCGCTC







CGGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CAMTA1. In some embodiments, the sequences from gene CAMTA1 comprise or consist of:











(SEQ ID NO: 5)



CGCCCCACCCTGTCCCCCCTGGTTGGGCTCGTACCGGCCAGGGCC







CCCTGGCCAGGAAAGGTCTGTAGCATGTATGTTTCATGTGTTTAA







TGAATTCTGTAACCATCAGCCATTCCCTGTACCCCAAAGGGTCTT







AACCTAGGCTCTAGGAATGAACTACAGAGTAACTCCAAGCCCCTA







AAATTATAGGAAAATGCATACGTAGGTACATTTCCCTGGTCCTTG







GCTGTCCTGGGTTCTCGACGCCTCTATGGCTCCCGGAGGGTAAGG







CCCCAGCTGCCCATCGAGTCTGCCGGCTGGTGTGTCCCCACGGGG







CTTCGTAGGTGCTGTCCCAGAGCCTTAGTCTTGGAGCTGGAGCCA







GACGCAGAGGTGCCCTCGGTCTCCCTGGGCCGCTGCCAGGCGGCT







GTGGCCCCTTCTTGCTCCTTCTCTGTTGCGCCGTCTTCCTTCCTT







CCTTCCTTCCCTCTTTCCAACTGAATTCTCGTTTTTCCTCTCTCC







CTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CAMTA1. In some embodiments, the sequences from gene CAMTA1 comprise or consist of:











(SEQ ID NO: 6)



TAGGTGGCTGGGACTGGATTCTTCTTCTGTCTCTGGTGCTCGGGA







AGGGTCTGTGGAGGGCACTGAGGGCGCGGGGCTCCGCATGCGACA







CGGCTTCGGGCTGAGAGCCCTGGTGAGCTTGCCTAGGGAGACTTC







GGCTGTTGCGGCACTGAGGCCAGCTCAGTGGCTGGGCTGGTGTTG







TCATGTGTCAGGAAGGGGAGAAGGCCTGGTGGCAGGGGTAGCTGT







CACCCACTGATGGGAAATAAACCTAGGTGCTTCCAGCTTCCAAGG







CGCCTCAGTTTTCTTTTGATGAGGACAAGAAAGAAGGCCGAGTAT







CCTGCCCTGAAATGCCTGTTGGCTGAAAGCTGTACATTCATTTTC







TCTCATGGGTTAGTTACAAGGCACATGTGGAAAACAGGCTCCCGG







AGGTTGCCAGGCACAGCTGGATTGATTGGGATAGCTGTGGGGAAA







GGAGGAAGGAGCTCCGTAATCGATTTGTGATGTCTGCTGTGGCAG







GGAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RERE. In some embodiments, the sequences from gene RERE comprise or consist of:











(SEQ ID NO: 7)



GTCAGTATTTGAAATTTTCTCCTTGAGTGGCTATTATAAGAGGAT







CCCGTAAGAATTGATTACTGTGAAATTAGCCAACTACATAGTGTA







TTTCAAAGATAAGGTAAACTGTTGGACTTGTGATTACCTGGTTTC







TGGTATGAATTACTGAACTTATGTTTGTTTTTATGTGCATAGATA







AAGCTATATGCTTGATACTCTTTCAATTATAAACTTAATGGGTTA







TAAGAATTTATAACTTGGGCTTCATTAAGTGAAGAAGGCGCTCTT







ATACTTTGTCTTTTTGTTTTGTTTTCTTTTTTTTTTTCCTTCTTT







TGAGACAGGGTCTTGCTTTGTTGCCCAGGCTGGAGTGGTGCAATT







ATAGTTCACTGCAGCCTTGACCTCCTGGGCTCAAGCGATCCTCCC







ACCTTGGCCTTCCAAAGTATTAGGATTACAGGTGTGAACTATCAT







GCCCGGCCTAATTTATCTTTGTTAAGCTTCACCTGTTCTGTTGAT







GATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RERE. In some embodiments, the sequences from gene RERE comprise or consist of:











(SEQ ID NO: 8)



GCCTCCTGGGTTCTAGCAATTCTCCTGCCTCGGCCTCCAAAGTAG







CTGAGATTACAGGCGCCCGCCACCATGCCCAGCTAATTTTTGTAT







TTGTAGTAGAAACGGGGTTTCACCGTGTTGGCCAGGCTGGTCTCG







AACTCCTGACCTCAAGTGATCCGCCCACCTCGGCCTCCCAAAGTG







CTGGGATTACAAGCGTGAGCCACTGCACCCGGCCTTCCTGCTTCT







TTATGTTCCGCTATCCGATCCTCACAGCTTCTTTAAACCTTCCTA







ATATTGAGAAACATACCCGATGTAAGACACTGTTAAAAAGAATCC







AGCATCTGGGGGAGGTGTTAGGGAATTGAATGTTGCTAAAGCACC







AAAGAGAGAGGAGTTTGACTATTTTGAGCTTGTCTGTGACACCTG







TTTTGTTTGGAAAATGCTAATGAAACTAGTTGGCAGAGCTGCATT







TTTGAAGTTGTTTCATTTAGTCACAGTGATAGTCATCTGTCTTCT







TTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ATP13A2. In some embodiments, the sequences from gene ATP13A2 comprise or consist of:











(SEQ ID NO: 9)



GTGAGGCAGCCCCATTGCCCCTTCCCAGAAGCCCCTGCTACCCTG







GCCTCCCCAACTTCCCCAACCCTCGGCCCCCCCATAAAGGCTGAC







TCTGGCGCCTCCCATCCTAGGGAGGGAAGATGGCTCTGTGAGCAC







AGGGAGGTGCCAGGGCAGCTCCCAGAGCTCGAGAGTAACCCTCAC







CTGCTTCCCCAGCCTGGAGCCGTCCCCACAGTCCCAGGCAGAGGA







TGGCCGGAGCCAGGCGGCAGTTGGGGCGGTACCAGAGGGTGCCTG







GAAGGATACGGCCCAGCTCCACAAGAGCGAGGAGGCGGTGAGTGT







CGGACAGGTGGGTTCTGAAAAAGTCCTTGCCCCTCTCTCGGCCTC







GGTTTCATGATCTGTGCAATGGGAGAGTCAGACTTCATGTTCTCC







CACCACCTTTTAGTTCTGTTGTCCCAAACATCCAGGATGCTGTCT







GAGCACGTTTACACTTTCAGGCCTCTCCCCACCTCTCTGTCTGGT







GACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ATP13A2. In some embodiments, the sequences from gene ATP13A2 comprise or consist of:











(SEQ ID NO: 10)



CTCGCTGCGTTCGCAGAGGCTCAGCGGCTACTGTGGCAGTCCATG







GAGGGTCATCGGCTATCACGTCGTGGTCTGGATGATGGCTGGGAT







CCCTTTGCTGCTCTTCCGTTGGAAGCCCCTGTGGGGGGTGCGGCT







GCGGCTCCGGCCCTGCAACCTGGCCCACGCCGAAACACTCGTTAT







CGAAATAAGAGACAAAGAGGTGAGAGTCAGGCTGAGCCTGGGGCT







AGAGAAGCGCAGTGAGGGTCAGGGTCGGGGGTGGAGGATGCTTGG







GTGCCAACCCGGCCACTGACTTTCCATGGGACATTAGGCAGGTCC







CGCTCTTCTCTGGACCTCAGCTTTCCCATCTGAGCATTGGGCAGA







AGGGACGGGTCGGGTGGTTTCTGGAGGGCTCTCCATAGCCTTGAT







TTCTGCCTTCTCCAGCAGGGGCCGCCTGGCTGTAGGACAGGGCCT







GGGAGGGGAGGGGACTCTTCTGAGACTGAGTCCATACCTCTGTGT







TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CAPZB. In some embodiments, the sequences from gene CAPZB comprise or consist of:











(SEQ ID NO: 11)



GTGAGCAGAACGCCTAGGGAGAGTGCTGGCTCCGCTCGTGGTGTT







TGTTTTGGTAAAGAAGGCAGATGCTATCCAGACTTTTGAGGAACC







TGGGGTTTCACATAGGCCCCCACAGATAGACCTGCCCTCCTGCCA







CTTGACCCTTCTGCAGGACAGGCCCAGCACTTAGGTTGGAAGGGC







CAGAGAACTTCTTCCTGACAGGATCAAGGGATTTTTGGAGCATGT







TTGTATGTGGACTTTGGGCCTCAAAGTGTTTTGAGGACCATGGTT







CCTTGTTTAGTCAAGGGATGTTGCTGCTGAACGAGGGTGAGTAGT







TGAGATGGGTTAAGCTTTCTTTTGATGCCTTCCTGTATGCTGATT







AAAAACATGGGCAATGCCACTTTTAAGGCCTTCGGATTTGTCCAG







TAACTTTGGCTGAAAACTCCTTGTTTGGGGAGGTGGGCCTGGCCC







CAGGCCTTGATGGAGAACGACTTCTCCAGGGCAGTGGTGGGTGAG







GACT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CAPZB. In some embodiments, the sequences from gene CAPZB comprise or consist of:











(SEQ ID NO: 12)



AGCTGGAGTCCCACTCACTACCTCTGTGTTCAGGCTGGTCTCTCC







CATAGCCCAGGCTAGACCCTCCCTCCCGAACCGTGTCCCCAGCCA







GGCACCCCCTCGGTGACCCTGGCGTGGGCCCCCTTCCTCCCCCGG







TCTAGGGCTAGGTCTGCTTTCCCCCGTCTCTCCGGCCCAGGCTAG







GCCGTCGTTTCTCCCTGCCATGGCCCAGGCTAGGCCGCTGCCTCC







CTCTGTCCCTCCCCCTTTGCCTTCTCCATGGCCTCAGGCTGGACC







CCGGTCAGCCCCTTCTGCAGCGGCCTAGGTTTGCTTCCCCTCTTC







TCCGTGGCCTCAGCCTGAATCTCCTCTTCTCTCTGTGAACTTAAA







TCTGGACCTCCTCTTCTCCGCCCATGGCCCCCGTCTGGACCTCAC







TGCCCCCTCCCCACCATGATCCCCTTCCCTGGACTTTTCGCCACT







CCTAGCGTAACCTGCGTCAGCCCCGCTCGGTCCCCGCCCTGAGCC







TCTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene KDM1A. In some embodiments, the sequences from gene KDM1A comprise or consist of:











(SEQ ID NO: 13)



CTAGGTGGAAATGGATGGGGCCTGGTGGCTGACAATCTCACATAA







TCTCATACAACTGAGCCTCGGGTTAGAATGGTGTACCGGAGATGC







AGTCCTGTCATCTGGTGTCAGTCAAATGTGGCAGCAATCTTTTGG







TTTGGTTTTTGTTTGTTTGATTTTGTTGTTGTTGTTGTTGTTGTT







GTTACTGTTCTGTGTTTTTCCTTTCCTCTTTTGAGTTTTGCTTTA







TTCCTCACTGCTTTCTGACACTTCCCCCTCACTCCCCTGTCTTTT







GTTAACTGCCTTTCTTTTGGCCATTTCTGTGCCTTGACTTTCCCA







TTTGGTCTCTGTACAATCTGTTCCTTATTTCTCTGGTGGTTGCAG







CTTCTGAGCTAACAGAATGCTTCTGTCATGCTATGTGTTTAAAGC







CTTGATTTTATTGCTGTCATACAAAACTAAACTTGATGTGGCTGT







TGGGCTGTTATTTACAGTTTATTTCTCTCTCTGCTGCACTGGTTA







AAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene KDM1A. In some embodiments, the sequences from gene KDM1A comprise or consist of:











(SEQ ID NO: 14)



TATTTTTTTCATAATTTTTCAAATCATTTTCCTCACTGTTTACAA







TAACCCATTAAAAGCTTGAAACCTAAATCTTTTCCTTTCAAAGAT







CCTCTCCATCATACATTCACACTGGTTTGTTGTTGTAGAGTTGCG







CCTATATGTTGGGCTGGTGCCATTTGAGTTTTTGTCATTGAAAGC







ACGTGTAAAACATTAGGTTTTTTGTGGGTGGTGTGGGTGGACTAA







GGGGGAAAGTGTAATGAATCCAGTGGGCTGCTCAGCAAACAGAGG







GAAGAAACAGGTCAAATTTGGTCCCTCTAACCTTGACAGTGTCCC







TTTAAATATTTCTCTACCACACACCCTGGACCCCAGTTACTGAGC







CTGAGTGCTGTGTACTTTTTCAGTATGAACTTCCCTTTGTGATTG







TCATCCTAGTTGCAAAGATTAAAGCTTGTCTGAAGCAAGCCATCC







AGTTGACACTGGAAGAGCTTTAATCTGTACCATTTGCACACTTGT







GTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene STMN1. In some embodiments, the sequences from gene STMN1 comprise or consist of:











(SEQ ID NO: 15)



GTTTGAAGATGTCAGTTGCCATGTCAATTTTTATATTATTATTTT







TTCCATTTCCTGGTAATGAGATGCTATGTTATCTTTATTTATTTA







TTTATTTATTTGTTAGAGACGGGGTCTCGCTTCGTCGCCCAGGCT







GCAGGGTAGTGGCGCAACTATAAGCTCACTGCTGTCTGGAATTCC







TGGGTTCAAGTGAACCTCCCACCTCAGCCTCAGCAGCTGGGACTA







AAGGCACATAATACCACACCCAGCTAATATTTAAATGTTTTGTAG







AGACTGTGTCTCACCATGTTGCCCAGGCTATTCTCCAACTCCTGG







CTGAAGCAATCCTCTGGCCTCAGCCTCCCATATTCTTTTTTAAAC







AGCTTCTTTAGAGCACAATTTACATACCATAAAGTTGACCTCTTT







TAAAAGTGTACATTGCGCCGGGCGCGGTGGCTCACACCTGTAATC







CCAGCACTTTGGGAGGCCAAGGAGGGTGGATCACGAGGTCAGGAG







ATCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene STMN1. In some embodiments, the sequences from gene STMN1 comprise or consist of:











(SEQ ID NO: 16)



GAAGCCATTTTTCCAAGGTCACATAGAAAGCTAATGATGGAGAAT







AGACTCAAACTTGCTGCACTGCCTGAGTTTTGGATTTTCTTTTTT







TCCTTCCATTCCTTCCCTCTGCCCTTCCCTCCCACTGTCCTCCAA







TTCATATTTGTCACATGCCTACTTTGCACCAGCCCAGTACCCTGA







CCTCCTTGCCTTAGAACCTTAGGCTTCCAGAGCCTAGGTCATCAC







CACCTCTTCCTATCAAACCTCCTCCCCACACCCCGTTCCTGCCTG







ACCTTTCCTACTCGTTAAAAATCCAGATGCCTAGGTTGCTTCCCA







TACCAACAAGATCAGAATGTCTGGGGGTGGGGGCCAGGTGTCAGT







ATTTTTTAAAGATCCCTGGATGATTCCATTTGTAGCAAAGTTTGA







GAACCACTGTTTTAGCCCTGATGAAGGGCCCCTCCACCTCCTGTC







CCTTGGCCTCTGGGTTGCAGATGACCTGGTGATTTTTCTTTTTCT







TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene EPB41. In some embodiments, the sequences from gene EPB41 comprise or consist of:











(SEQ ID NO: 17)



AACGCCTACTGTGTACCAGACCTATGCTTGACATTTAACTTACAT







CGCTAATTCTCAGGAAAACATTGTAAGATTTATGGTTGACGAAAT







TCAGATCCAGAGAAGTGACTTGTTCAGGGTCACGCCGGACGAAGT







GGTAGGACAGGCGATCCAGGTGAGTCTCCTCAGGGTCACTTCCCT







AGATCCCCTCTGACCAACCCCTCTACCTAGTAACCTTAGGAGCAG







TACCACGTGTCAAATTGCTTCACACTTTTGTCATATGACGTCACA







CCAGTCAATCCAGGAACTCCCTGCCCTTCCCTCCCCTGACCTCTG







GGGTCTATCAAAGCAGCCCCCGGCTCAGTGTCCCCGCCGGACGAG







GCTACACCTGCAGCCCCCGCCCCTTCCCAACTGCGACTCTCGCAC







TCCTCTGCCCGGGCCTCCCCCTCCCCGCCCCGCTGATCGGCAGTT







GCCTGCGCCAATCAGAGGGCCTAGCGGGCGGGGCGGGGCCTCACG







ATGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene EPB41. In some embodiments, the sequences from gene EPB41 comprise or consist of:











(SEQ ID NO: 18)



TGAGCCTGGACCACCTGGGGGGCGACCCTCGGTCCCCGGGAGGGA







CGGGGTTAGGGACAGAAGAACCTACCCCCAAGGGCTCGGACCGTC







CCGGGAGAGGCGAGACCAGGGTCGAAGGGTCCAGGGCTGAGGGGT







CCAGCGGTCCCGAATTCCAGAATCCGAACTTGGGGTCCAAAGGAG







TCTGGGCATCTTAAAGTTCAGGGTGCAGGGAGGGGCGTGGGAGTC







TGGAGAGGGGGTCCGGGAGCTCGGATCCGGAGCTAGACACGTCCG







GGTCCGGCCGGTCCTGGCTGTCTGGGGCGGGGGTCCTGCATTCGG







TGTCCGCGGGAGAGTCCCTGCAGGTCGGCGCAGCCCCCGGCCGCC







CCCTAGCCCCGCCTTGCCCGGCCCCGCATGCTCCTGCCGGGCCCG







CAGCAGCGCTGGAGCGGGCTGGCGGCTAGCAGCGGGAGGGGGCTC







CGGGGCCTGGAGCCCCGCGCCCCGCTCCGGCCCTTACGTAACTGA







CTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TRAPPC3. In some embodiments, the sequences from gene TRAPPC3 comprise or consist of:











(SEQ ID NO: 19)



GGGCTTTAACATTGGAGTCCGGCTGATTGAAGATTTCTTGGCTCG







GTCAAATGTTGGGAGGTGCCATGACTTTCGGGAAACTGCGGATGT







CATTGCCAAGGTCATTATTCTGGGCCACCTGTCCATGCTGAAGAA







TTGCTGCTGAGAGGCACTGCTTACCACTCTTTCTATCTCCCTTAG







AGAGGCCTTTAATCTCCCAAAAGACTGGCTAGGCAGCTCTTTGGG







TCAAAAAAACATTCCAGGTCGTTAGGGAATAGCATAGATTATTTA







ATCCCTGTGGATTAAAGCCCCACTGAAATCAAAGCCTGAGCTTTT







CCACTGTGAAGGTGTAGCAGAGTAGCATGGGGGTAAAGAATACAG







ACTCGGTCGGGCGTGGTGGCTCACATCTGTAATCCTAGCACTTTG







GGAGGCCCAGGCGGGTGGAACACAAGATCAGGAGTTCAAGACCAG







CCTGGCCAAGATGGTGAAACCCCATCTCTACTAAAAATACAAAAG







TTTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TRAPPC3. In some embodiments, the sequences from gene TRAPPC3 comprise or consist of:











(SEQ ID NO: 20)



CCCTGCTGCTTTGACCTTGCAGGCCAAATTTCCTGGCTCCTCCAA







AGGGGATGATGTTCTTTCCCTCCAGACACAGTTAGAGGGAATCCT







GACAGTTGCATCAAGGCTGGTCCAGACTCTCACCACGCTTTCTCT







TTTGTGCTGCTTGGTCCTGACTGCCTTTTGAATCTCTTTTAGAGC







TCTGAGCTCTTCACCCTGACCTATGGTGCCCTGGTCACCCAGCTA







TGTAAGGACTATGAAAATGATGAAGATGTGAATAAACAGCTGGAC







AAAATGTGAGTGAGCTCCTTCATAGGAGAGACAGAAATGGGGCCT







CCCAGCACACTGAGTCTACATTGTCCTTTAGAGCTTAAAACAGGG







TTCTCTCTCTTTCTTAGCCAACATTTTGGGTACATTTCTCACTAA







ACTCATTTACTGGGATGTTTTTGAGATTATCCCCATTTTCCCTTT







ACCACCAACAACCTTATGTTCAAACATAAGACATTTAGACTCCAT







CATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CDC20. In some embodiments, the sequences from gene CDC20 comprise or consist of:











(SEQ ID NO: 21)



GTGGCACTGGACAACAGTGTGTACCTGTGGAGTGCAAGCTCTGGT







GACATCCTGCAGCTTTTGCAAATGGAGCAGCCTGGGGAATATATA







TCCTCTGTGGCCTGGATCAAAGAGGGCAACTACTTGGCTGTGGGC







ACCAGCAGTGCTGAGGTGCAGGTGAGACGTGTCCAGTGCTGTCAT







TCTCACCAGTCCCAATGGGCTTGCACAGCTGGAGGATATTAATGC







CAAGTCCCAATCTCTGATCCTAGTGGGCTTCTCTCTCTAGCTATG







GGATGTGCAGCAGCAGAAACGGCTTCGAAATATGACCAGTCACTC







TGCCCGAGTGGGCTCCCTAAGCTGGAACAGCTATATCCTGTCCAG







GTCAGTGGTTTTTGTTGGTCTATGGTAGTCTGATATTTGCCCACC







CTCCCCTTGACTGTACACCCCTGAACTGAACCAGCTCTGGCTTGC







TTGCATTTGGTGCTGCCACAGAACCTGATTCCCTTCTTTCCTCCT







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CDC20. In some embodiments, the sequences from gene CDC20 comprise or consist of:











(SEQ ID NO: 22)



TGAGTAGGGTTGGGCTGAAGCCTCTGCAGACCCTCACCTATAATC







TGGGGACTGTTAAGGGGAGAAGGGATGGTAGGATTGGACTGGGTT







AATCTGTGACCCCTAGAGCTCCTTGTCTGGCTCTAGTACCTGCTG







ACCCCACCTTTATTCCATCTAGGCCGTAGCATGGTGTCCCTGGCA







GTCCAATGTCCTGGCAACAGGAGGGGGCACCAGTGATCGACACAT







TCGCATCTGGAATGTGTGCTCTGGGGCCTGTCTGAGTGCCGTGGA







TGCCCATTCCCAGGTAATCTTTTGCCTGTTCCTGCCTCTCCCACA







TGCATTCTTACCGGGCAACTACATTCACTCCAATGGCTTCACCAA







CTCTATGCCCTGGTGATTCCCAAACTTCTAACTCAGCTCTCACTC







ATGAGCTTCATTTCCAACAGTCTGTGGCTATCTCCAATCCTATGT







CCTATAGGCACCACCACCTTGATCTGTCCCAAATTAACCTTGTCA







CTTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene B4GALT2. In some embodiments, the sequences from gene B4GALT2 comprise or consist of:











(SEQ ID NO: 23)



GGATGTATGGCTGTGGAAGTCCAGGAGCAGTGGCCTTGTTTGTGA







GTGTGGGACTTAGTGTGTTACTGTCACCCGGGTGTGTCTGTGACT







GTAGTTCTCTGTGTGTCTGAGTGTGGGTGACCAGCTGGGACCAGG







GTGTCCCTGTGATTCTGTGTGCCTGTGAGACTCACCCTGCTCCCT







GAAGCACTGCCAGAAGTGGCTGCCGCTCTCTTGGAACCACAGCTG







GAGCCATCGCCCAGGCAAGACCCCCTTCCTGCCATGCCCAGTGCT







GTGCCCATCTCCAGGCCCAAGCATCCCGTTCCGGGAATCAGAACC







AGCTGTGCTGGGCAGGACCCAGAGTCTTGGTCCCAGTCCCAAGGG







GCTGGCTTTGAGGTCTTAAGTGAGGGGTGTCCCCTGCCTTGCCCC







GTATTTTGACGTTTTGCTCCAGTGGGAAGGGATTCTGTAAACATC







CAGTCTGTATTTGGGCCCCCCGGATGCTGAGGGCAACTTCCCAGC







CTGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene B4GALT2. In some embodiments, the sequences from gene B4GALT2 comprise or consist of:











(SEQ ID NO: 24)



TAGGTGCACAGTATTCCGTCTCTTCCCTCTTCCCCAAGCCATGAG







TTCTGGGTTAAGGATGCTGCCATGTGAGGTGTCTGTTCTCAGGGG







CACAGGGGCACATGGGGGTCTATAGGTAAACATGTCTCCATGCTT







AGGTACAAACCAGTGTGGTTAGAGTTCACGTGTCTGTGTTCCTGG







GAATGCGTGAGTGTGTGTGTAGAGTTGTGCAATAAGGGTGAGTGC







CTGAGTGTGTGTGTCAGTGAGGTGGAGGAAGGTAAATGTATGTGG







CTTAGTAGTGGGGTGTGCTTGAGGATGTGAGTACTGAGTTGCCGG







GAACTGTGCCTGGGTGTCTGTTTGGAGGTAAATTCTTGAGTCTGT







GAGTGTGAAAGGGTATGAGCAGGTCAGTGTGAGGTGTGGCCCACG







AGTGTGAGCAGCTGAGTGGGAGGTAGGTGGGCAGCCTTGCCTGTC







CTGCCCTGACCTGCTGGATCTGTTTCCCTCCCACCCTGCTCAGGC







CAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RNF220. In some embodiments, the sequences from gene RNF220 comprise or consist of:











(SEQ ID NO: 25)



GAGAAGCATTCCAGGAACAAAGCAGCAGGTAAGATTTTTCTGGGA







AAAGACAGAGATTTTGAGGGACCCAGGATTTGGGGGTCATTTAAG







CAGCAGTGGATGTCAGTCTTCAAGCTTTGCCCATTTACTCTTGGT







CCCTTTTGGTTCAGGAAAGGAAGAACAACAAGCACGAAACCATTC







ACTGAGACCTTTGCGGGTGGGGGAGGAGGGATGTAAACAAAAGCC







AACGCTCTCTTTAGTTCACAATGGCTCGCGACCCCAAGACTTCCA







AGCTCTTTGGTGAGGGCCAGCAGGTCTGTTCTCCAGGAGTGCCCT







GGGTCTGGACTTCATTTCCCAGAGTCCCTTAGTGCCCTAATGTAT







TCAGTGCTCAGAGTGCCTGTATGTGTGTGAGTGTGAGAGAATGTG







TGCGTGCGTGTGTGTGTGCGCGCGTGTGTGTGTCTCCGAGTCCCT







GGGAGAGTGGAGGCTCATTCACTGATTAGATCCAGCGCTGAGAGG







CAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RNF220. In some embodiments, the sequences from gene RNF220 comprise or consist of:











(SEQ ID NO: 26)



TATGGAGATCAGAAAGGGGTGTGGACGTGTGTGCGTACCCAAGAG







GGGTGGGTGCGAGGGCGGCCGGCTTGTTCAGGAACCTGGCTAACT







TTCCGGGTCCGTTTCCTAGGGATTCGGGCGGTTCCCTCTGGACGG







GGGCAGGGACTGGGACTCGGACTGGCGCGTCTTGGATCATCGCGA







CGGAGCCTCTCGGCATCGGCGCCCTGCGCGCTCCGTGCGCTGCCT







CGCTCAAACTTTCCGTCTTGAGTTTTCCTTAATGTGTTTTATTTG







GGGAGGGGGGGAGGTGCAAGGGGGAGGGACCAGACGCGGGAATTG







GACAAGTTGGGAGGGTGAGGGCACTGCAAGGGAAGGGAGCAGGAC







TTCTTTTCTCCGTCTCTTAATAAGCCCCATTCCCACTTTGTTCGG







GTCGATATCGAGAATGCCTTCTCGCCGGGCTCTCTGGTGCACCCC







CCGGTGATTTCCGGGCTCCCCTATGTACCCCGCGTTGATTTCCGG







CGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PRDX1. In some embodiments, the sequences from gene PRDX1 comprise or consist of:











(SEQ ID NO: 27)



GCAAGCGTGGACTCGAGTCGAGTCTTCCCCCGGCAACTCGAGCAC







GTGGCGCGGCGTCTGGGCGGGCTGGTGGTGGGGGTCTCCGAGACT







AGCCTCCTGCCGGACGGGGTGGGTTCGGGAAGCTTCTGGAATGGA







CCTGTATCCAGACTCGTGGCTGTAGAAGACAGCCTCTATGTATTT







GGCTGTTCCGGGTCCTGAGCCTTGACTAGCTGCGGAGCTTCGGGT







ACCCTTTCCCCGTCTTTATTTTCCTGTACGTGGAATGGAGTTAGC







GATGAGCCCAAATGTCCCCGCTGAAGCACTCTCTCTGCTGGTGCT







TTCTCCAGTGCTGGGGAGGGACAGGGGGCACCTGAAGTGGGGTTT







AGGGATGTGGCCCTCTACGCTGGGCAGATCCCCTGAAGGGGCCCG







GTGGCGGCCTGAATCCCAGCAGCCTTTACCCCGAATTGGTTGGGT







TTTGTACTTGGGGGCCACTTGGGAAAGAAGGATTGGACGAGAAGG







CCAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PRDX1. In some embodiments, the sequences from gene PRDX1 comprise or consist of:











(SEQ ID NO: 28)



AAGCGTTCGGGTTCGGGAGCGGCCACAAGGGCGCCTCACTCCAAC







CTCAGCCATCCGCAACAGGGTGGGGTATTAGTATTTTTCCGCACC







CGCCCCAACGAGGGAAAGCGCCGAGTCATTCCGGCGGGGCGCGCC







GGGCGGGGCTCGGGCCACATCCCCCTCCTCTCTCTCCTCCTCCGG







CACCCCCGTCCGTTCCGACTCTCGCGAGATCCCTACTGGCTATAA







AGGCAGCGCCCCGGAGAGCTCTTGCGCGTCTTGTTCTTGCCTGGT







GTCGGTGGTTAGTTTCTGCGACTTGTGTTGGGACTGGTGAGTGTG







GGCAGTGCGGCCCCTGCGGAGTGAGGCGCGGCGCGCCCTTCTTGC







CTGTTGCCTCTTCCTCCTCCTGTCCGGGGCCCGCCCGCGCTCGGG







TGGGGGTGCTGTGATGCGTGAGGCAGCCGGGGGAGGCCCGGAGTC







CGAGACTGCTTGAGCGCTGCGCACACCCCTCTCGTGGGCCCCCCA







CGTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ATPAF1. In some embodiments, the sequences from gene ATPAF1 comprise or consist of:











(SEQ ID NO: 29)



GTAGTTACAGTAGAAAATTGGGATTCTACATTGTGTTTTAAAGTC







AAATTAGAGGTGTTTATTTTACTTGGAAAATACTTTTTTTGCCTT







GGGTATCCTGAATTTTAAGTGAATCTGCTGAGTCTTAATGACTAC







TACTCATCTGACAAAGGGCTAATATCCAGAATCTACAGTGAACTC







AAACAAATTTACAAGAAAAAAAAAACCCCATCAAAAAGTGGACAA







AGGATACGAACAGACACTTCTCAAAAGAAGACATTTATGCAGCCA







AAAGACACATGAAAAAATGCTCATCATCACTGGCCATTGGAGAAA







TGCAAATCAAAACCACAATGAGATACCATCTCACACCAGTTAGAA







TGGCAATCATTAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGAT







GTGGAGAAATAGGAACACTTTTACACTGTTGGTGGGACTGTAAAC







TAGTTCAAACATTGTGGAAGTCAGTGTGGCGATTCCTTAGGGATC







TAGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ATPAF1. In some embodiments, the sequences from gene ATPAF1 comprise or consist of:











(SEQ ID NO: 30)



TGAATCCTAACCCTTCCACTTACTAGCTGGGTGACTCTGAGCAAA







TTGTTACTTCTCCATGCCTCAGTTTGCTTATCTGTAAAACAGGAA







TAATAACAGTACCTACTTCACAGAGTTGTGCTGAAGAGATAATAC







CATTTAAAACTCTTTAGCGCCGTCTCTGGCATAAGTACTTAATAT







ATATTAGCCATTATTATCCTTAAATAGGAATCAAAGCTGAGGAAA







AGCATGTTGAAAACTGTGTCTGGGAAGATTATTCTAATGCAGTGT







TTTTCAAACTTTTTGACCATGACCACAATAAGAAATATATTTTAC







ATTGCAATTCAGTACACATGTAGGTATATGATTGTAACTGAAAAA







AAAATGTATAAAATAATGCTAACTTTTACAACATGCAGTCCATTC







TTATTACTTTTTATTCTCTTTCATCTTTTAAAAAACTCTGATCAC







AATCCACTAAATGTATTTCACAACCTATTATTTGAAAAATTCTAA







CCTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PSMA5. In some embodiments, the sequences from gene PSMA5 comprise or consist of:











(SEQ ID NO: 31)



GTGAGTTGGCTGCCGGTGAGTTGGGTGCCGGTGGAGTCGTGTTGG







TCCTCAGAATCCCCGCGTAGCCGCTGCCTCCTCCTACCCTCGCCA







TGTTTCTTACCCGGTCTGAGTACGACAGGTCCGTGCAGCTGGGGG







ACGTCGGGTGGGAATTATGGGTGGGCAGGCCCGGGACCTGGCCGC







GCGGCCTGGGTTCTGCCGTGTCATGGGGCTCCCCAGGAACCCGAG







ACTGGCCGGACCTGGGGATGTTATGCCCTCCTCAGGCGCCCGCCG







TCCTTCCCCAAGCTAGACCACATCGGCGGCACTTGTCCTACCGCT







GAGTCACCCCTGGGTCCTAACTCGGTGCGGAAGCGAGAAGCTCCG







GCTGGTGCTTGGTAAAGAAGGCGTGAGGAGCCGGAACTCCTACCT







TGAACCCGGAGAAGGCCAAGGGTCACTCTTTGCTCCGTTCATCTT







TTCGTGCCTAGTATGACTGCCTTGAACAAACAAACAAAAAGTTAA







ACGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene AMPD2. In some embodiments, the sequences from gene AMPD2 comprise or consist of:











(SEQ ID NO: 32)



GGAGCAGAGCCAGAGTGTGCAGAGACAGAGGAAAAATCCAAAGAG







GCTAGGGTCTCTGTCCCAGACCCCCAGACAAGGGGGTCTCCTGGC







CTTCCTGCCTTCCTGGGAGGTAGCTATTTGGCGACGGTGGAATTT







TCCCGGGACAGGCCCCTCCCTCCTGGGCTGGGATTCTCGGTTCTT







CCCCCTCCTCCCAGAGCTGGGCTACCCCGGGAGCTGAGGGTCAGA







TGTGGAAGGAAAGTGGGTGCTGGGTTTTCACGGAGTATGCCTGCC







CTGCCCCGGGAGGACTGTGTTTGAGCTTGGACTCGCTCGCTGCTG







AATTCCTGTTTGTTATTCTTTTTTCTTCCAGGCTAGCTGGAAGGA







CCAGCTAGCCTGGACTTTGGCTTCTTTGCCCGGGAGCCCTGGCAT







GATGGGGTGAGGGCTCTTGGTGGGTTAGTGGAGAGCTGGGGGCTG







GCCCTTCCCCATCTTCTTTTCTACCCACCCCCTCCCCGCCCCCCG







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene AMPD2. In some embodiments, the sequences from gene AMPD2 comprise or consist of:











(SEQ ID NO: 33)



TGAGTGTGTGCTTCCTGGCCTCAGGATAGATGCTGGGCTCTGTCT







TGCCACCTCTCGCCCAAGGTGCTGGTCCCCTCAGAGGGGGCCACC







TCCTCCGGCATCCTCTCTGTGGTGGTGCCCCTCAACCTCAGTCCC







AGGGACAGCCGGCTTGGAAGGTGGGGTGAGGGGTGGTGGGGGGCG







GGGGGTGGATCTGAGATATCTGTGCCAGGGATGCTGTCCTCTGTC







ACAAGGAGTGGGCATGGTGTGGGTGCAGCAAGAGGTGTGTATGCA







CACGTGTCAGCGTGTGCATGTGCTAAGACTGCGTGTGAAGCATGT







GTGAATGGAGCGGGGGTGTGTGGGACTGACTGTTTTGTGGACTGG







CCACTCTGACTGGCGCCTGTGCCTTTACCAGGCAGGCTTAGGCTG







TTCTGTGGCTAGTTCCACTTTTTAGGACCCCACATCTAGATCCCC







CTCACTGGGGGTCTGACATCTGGGCACAACCATTGCTGGCTGGCA







TCCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene AHCYL1. In some embodiments, the sequences from gene AHCYL1 comprise or consist of:











(SEQ ID NO: 34)



GTGACGTCACGACAGAGTGCCCTTTCTCCCCGCCTCTTCCCCCTC







CCGGGAGCTGCCAGTACTTGACGTGGCGTCACCGCCCTCTACCCT







CGCTTTGCGTGCGTGTTTGCGTACAGCGGAGGTGGCGGCGCGGGC







AGGTCGGAGCTCGGAGCTGCTGTTCTGGTTCTCTTGTGGCCGCCG







TCGCTGTCCGGCTGCCTTGGGCTGCCGAACAGACAAGGCGTGGGC







CACAGCACCTCAGAAGCCGACGCAGCTCGACGCAGGGGCCGGCAG







GAGGGTGGGCGATCGCGTGTCGGAGGGCGCCGCGCGGGCAGGCGG







GCGGGCGCCAGAGGGGGAAAGAGGCGGGGGCGGCGGGTCAGCCGC







TGGCCGGGCCGGCCGGGGAATGTCGATGCCTGACGCGATGCCGCT







GCCCGGGGTCGGGGAGGAGCTGAAGCAGGCCAAGGAGATCGAGGA







CGCCGAGAAGTACTCCTTCATGGCCACCGTCACCAAGGCGCCCAA







GAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene AHCYL1. In some embodiments, the sequences from gene AHCYL1 comprise or consist of:











(SEQ ID NO: 35)



TGAGTGGGTAAATCTGGCAGAACTTCCATCAAATCTTTGGGAGCA







GTGGCCGCTTTATCCAACAACTGCGTGATAGCTGGGAGGCAAGAG







AAAAGAATGCGTGAAGCTAGCTCTGATATACAGGTGAAAGCGATG







AGGGTAACATGAAACTGAAAATCTATGATTTTCACTTTCTACAGC







ATTCCAATTACTTCGAAATATGTAAGAAATTGACATTTGGAGGTT







GGTGACAGCAGACACTGTCATGGATACAGGTGGATCCTAGAGTCA







TGTGAATAGATCACTTAGGCATCCCTGGAGAAAGGAGCTTGGGTG







GGTTGGAGTAGTGGGTGGTAAGGGAAGATTGCTCACTCTTGACTG







TACTTTTGAGGCAGAACAAGTTTGATCTATTGTGCTGTAGGTTGA







CTCAAAGGACACCCTTCGAATTCTCTCATAAATGGCAACCTTTGG







AGATTTTAGTCTGACTAGCTCTCCGAGTGGTGGAATTGGGATCCT







TATT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SV2A. In some embodiments, the sequences from gene SV2A comprise or consist of:











(SEQ ID NO: 36)



GTAGTTGGAATGTGTTGGAGGCAGGGCAGTGGATTCAGGGGAGGG







GTGGGGTGAGGAAGGAGGGCTGAATGGGAAGAGCATCCTTAGGGG







TGAGAGGATATACATTTCCATTTTCTCCTCTAAAATCTGGTAGCT







TTGGCCGGGCACGGTGACTCATGCCTGTAATCCCAGTGCTTTGGG







AGGCCGAGGCAGGCAGATCACCTGAGGTCAGGAGTTTGAGACCAG







CCTGGCCAACATGGTGAAACCCTGTCTGTACTAAAAATACAAAAA







GTTAGCTGGGTGCCTGTAATCGCAGCTACTCAGGAGGCTAAGGCA







GGAGAATTGCTTGAACCCGGGAGGTGGAGCTTTCAGTGAGCCGAG







ATCGCACCACTGCACTCCAGCCTGGGCAACAGAGCAAGACTCAGT







CTTAACTGTTAACCTCAATTTTTGGTCTCCTTTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SV2A. In some embodiments, the sequences from gene SV2A comprise or consist of:











(SEQ ID NO: 37)



TCTCATGTTTTCTGTTTAGGGGGTGCTGCAGGATGGGGTGGGGAC







TGGCACAGAAGCTTCTCTGGGGGTAAAGGAGGTAAGCAGAATTGC







AAGTGGTTAGGGCACAGGCTTTGAGGTTATGGGTTTTGAATCTCA







GCTCCACCACCTGCCTCTGGCCTCTGGCAAATTACCTCTATGAGC







CTGGTTGTTTCCTCTGCAAAATGGAGGTGATAATAGTACCTGTGT







CATAGGGTTGATGTGCTAGATCAATGCATGTAAAGCATCAATTGC







CTTGGCCCATGGCTGGCATTGAATAAATGATGGTTACTGATATTA







TTAATAAACAATTATAAGGGAGGTTGAGGAGGAGTGAAAAGCCTT







CCTTTTGGATACCTTGGTTGGATACCTTCGGAACAAGTCTCATCC







TGGGCTATCTGAAAGCCAGCTGATGGGACAGGGTGGGCTGTGGGT







GGGTTTCACTGTGGCCAGGCTCATGTTGCTCTCTCCTCTCCTGGC







TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene S100A13. In some embodiments, the sequences from gene S100A13 comprise or consist of:











(SEQ ID NO: 38)



AATCGACCACGGAAATTTGACACCTCCGGGCTTGGAAGCAGCTCT







CTCCTCCTTCCCCGCTGCTTATAAACCTCAGCCCTGAGGCTCCAG







CTCACTCTACCCCATCTCCTTGCCGGGTGAGTCTCATGGCTGACC







CTGGAGGGAGGCTGGAGATCATTTTTTCTTCCTGGGCTCCCAGGT







CCCTCCCCCAGAGCCAGCTCAGACAGGAATTCAGGAAGGAGCTTT







GGATGATCGAGCAACAGCGCGTGCAGGAGTGTGTGACGGGGTGGA







CAGAAAAGAGAATGCCTCTTCCAGCCCCACCCGCCGCACCCCAGG







GGGTTGTGAGTGCCTGTACTAAACCCGTCCTGGAGCAGGGAGTCA







GAGACGCCCCGTTCCAGGACCCTTGTCACCGGGAGACAGGAGCGG







GGAGGATCTGTGGGGTCCTGGGTTCAGCACTCTGCCCTGCTGCCG







CTGAGGAGAGGTAAGGGTGGAGCGGGGCAGAGAAAATGGGTCTGT







TCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene S100A13. In some embodiments, the sequences from gene S100A13 comprise or consist of:











(SEQ ID NO: 39)



TGGCTGTGCTGGTTGAGCCCGGTAAGGGGGGAATTCTGCTGAGGG







GGTCTTAGTCTGTGGCTCCTCCATGGGGCCTCACTGAGAGGACCC







TCAGTGAGAAACTTAGTCTTGGGAGTCTGAGAACCCACAGAGGGA







TTTCAATCCAGGACTCTTCTCTGGGGGTCTCAACCTGGATCCCCC







TCTGTGTGTGGGGCCTCAGCCTGGGGTTCTCTGAGGGATCTTGGT







CTGTAGCATCCTTTGTGGGATCTCATTCCAAGGGGCCTCTGTGTG







TGTCTCAGATTGGGCCTCTCAGTGTTGAGCTCCCTTGATTGGTTG







CCTCCCTCTTCCCCCCTCAGCATACACACTCCTGATTAGACATGG







GTATGGTGGCAGCAGCCCCCAGTATTGAGAGGACTAGGACCCTGG







GACCCCTGCCGAACGAGATCTGTCCAGGCAGCTCCTCTCCCTGAC







CAAGTGGGGGCGCTCGTGTGGCTGGCAGGGAGCCCGGGTCCAGAC







CTGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene UBAP2L. In some embodiments, the sequences from gene UBAP2L comprise or consist of:











(SEQ ID NO: 40)



ACTGAGGCACGAGAATCGCTTGAACCTGGGAGGTGGAGGTTGCAG







TGAGCCAAGATCACACCACTGCACTCCAGCCTGGGCAACAGAGCG







AGACTCTGTCTCAAAAATAAATAAATAAATAAATATAAATAAATA







AAAAATAAAGGACTTTACTTTTTGTAGTGATATGTATAAAATATT







TAAACAGAAGTAAAAGCAAACTAATGGAAGTGTTTTAGACATGGA







TGCTGAGTGGAGAATGGTTAAGTCAATCAGTCATCCCAGCTTTCA







TCTCTAGTGTCCTGATAGCACTCCATATTGATTAGATATGAATGC







TCTACCCTTCTATAGCATTCCTGGGAGATGGTCGGGAAGAAGAAG







GGAGTCTCAGGCCAGAAGGATGGTGGCCAGACGGAATCCAATGAG







GAAGGCAAAGAAAATCGAGACCGGGACAGAGACTATAGTCGGCGA







CGTGGTGGGCCACCAAGACGGGGGAGAGGTGCCAGCCGTGGACGA







GAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene UBAP2L. In some embodiments, the sequences from gene UBAP2L comprise or consist of:











(SEQ ID NO: 41)



TCAGTAATGTCTAGACTCTAGGGATGTGCCAGGGCCCTGCTAGTC







AGGACCTAGGAACCATTATATTATCCTTTTGCTTTTCTTGCCGTC







GTCTGTTCTGAGACCTTGACATCTCTTGCATGTGGCCTTTTCTAC







CAACTGCCACCAGTCTGGCATGAGATTACCCTGTAGTCATTTATA







AAGTTAAATTTGACTTGCAGCTCTCCATTAAGGCACATTACCATT







CCCTGAGAAACATGTTCCCATCCTTCCAGCTCATTTTCTCTTCGT







TGTTTTTTTAATTCCCAATACCATATTACATACCATTTATATATT







TGTATATATCCTGTTGACTGTGCTCTGGCCATCTGTGGTTTGGTT







TTTTTGTTGTTGTTGTTGTTTTAATTTTTATTTTTATTTCAGTTC







GAGGTCAGGAAAATGGATTGGATGGCACCAAGAGTGGAGGGCCTT







CTGGAAGAGGAACAGAAAGAGGCAGAAGGGGCCGTGGCCGAGGCA







GAGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ADAR. In some embodiments, the sequences from gene ADAR comprise or consist of:











(SEQ ID NO: 42)



GTAAGAGCCTCTGTCCTTCTCGGCTACACCTGCCTGGGCTGGAAC







GCGCGGCCCATGCGGCCTCTCCAGTCTCTGGCGTTGATGTTAGAG







GAAGCGTGGGGGCGCCCCGGCAGGGCACTGAGGGTGGCCGCAGGG







CTGGGTGGGGACGCAGCTGGTAGGGGCACAGTGGCCGGTCTCGGC







AGCCTTCCAGGAGGCGGACGCCCGGGCCGGTGTACTTTTGTGCGT







GTGTGCGCGCCCGTGTGTGCGCGAGTGTGGGCGGCAGAGGCTGCG







CACGGATGCTCCGGCGCTCGTGCCAGCCGGAGCCCAGCAGCTGGG







TACCAAAGGCCCAACAGCTGGGTACCAAAGGCCCTTGTTTCCCTC







TCGCCGGCTCCCCCGCCTCGGAGAGTGACTGGAGAGTGAGCTGCC







TGGGACTCGCCGCGGTAGGCGCTTTTGCTGCGCCTTCTACCACAA







ACTGCGTTAGGACCGGCCCTTTATCCCAGAGATAACATCGCCGCC







CTGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ADAR. In some embodiments, the sequences from gene ADAR comprise or consist of:











(SEQ ID NO: 43)



TTCAGGATGGGAGTTACTTCTGATGGAGAGGCAGTGGCACAAGAT







GGGGACCACTTACAAGCTGATGAAAGTTAATTGATAATATTTTAG







TTCTTGGTTAGCATGTCAAGTTAAAGAAGGTGTTCATTATATTAT







TTTAAAAATGAATTCGTTTAATTTAATTTAAACGGGCCATTCAAA







GACTACTGGTTATAATGTATTATTGGCTAATTCTGTGGTCTTTTT







AAAAGAGGATTGTACCTACGTATCTTCTCAGGCCTTCCGGCCCAA







CCACGTTATAAATGACTAAGGAGTTCAATCAAGGCCATGGGCAAA







AATGCAGCATCCTAAACCCATGTACCTACTCCCCACTGTACCCAA







AACAGCGGCATTAACACAACGTAAAAAACCAAGGAAGACGTAGCA







ACAAAAACCTTAATTTCCTAGAGCGCAAACAAAAGAGGTTGAATC







ACGTGGTTCTTCCTCCCACGCCCGGCGCGCCAATGATGTCACCAA







TCTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ARHGEF2. In some embodiments, the sequences from gene ARHGEF2 comprise or consist of:











(SEQ ID NO: 44)



GTAAGGGACGGAGCGGGAAGAGGGAGGTGTGTGTCTCGCGGGTGG







GGGCGGTGGAAGCTCCGGCACCTCCTCTCACCCCGGGCCCCACCG







GCTTGGGCTTGGCGTGGCGACCCCCCATCACTCCCCAATCGCTGG







CTTGGTCGTTCCCCCTTTGTCCCCGCCATCCGGCGGAGGGGACGG







GAGGGGGTGGTTTCCCGGCTTCCTCCCACCACCACCACCACCCCT







CCAAGACAAAAGTCAGCTCCTCCTCTCCTCGCAAATGGACTTTCC







AGCTGTGATTCAGTTCTCCCCTCTGGGTGGGCGGGTGGCTGGGGT







CGGGACGGCCGAGGGACGGCTTACTTCCCCCAAACCATCACCTCT







AGGTGAAGGGAGCAGAGGTCTTTGTGTAGCTGGGCTCCTTTCCTC







CATCCTGAGCCCTTCTGCCTGGAACATAGGGCCTCCATCCCTATC







TGCCCCGCCAGCCTCCTAGCTCTTCCTTCTCCCAACCTGAAACCC







TTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ARHGEF2. In some embodiments, the sequences from gene ARHGEF2 comprise or consist of:











(SEQ ID NO: 45)



ACAAACCCTGGCGTCCCGTCCTGCGCGCGGGCACCCCGCCCTCCC







TCCGCTCCGCCTACCCCCTTCCTGAGCTTGGGCGGGGGAGGAGAC







TCGGGTTTGAGGAACTGGTGCTGGGTGCCGGGCGAGGGGAGTCAG







ACTTCCTGTCCCCGAGACCAACGCGTGCGGGCCGAACCCCTCCCC







CCGCCTTCCCCCAACAATACAGGACGCCGGGGTCCGCGCCGCGTC







CTCCCTGGTCCCCCCGTCCGATTATGTCTCGGATCGAATCCCTCA







CGCGGGCGCGGATCGACCGGAGCAGAGAGCTGGCGAGCAAGGTGG







GCTCGGAAACCCCTCTCCTTTCGCCGCGGTCCTCGGGTGCTCCCC







GAACCCCGGCACCCGCGGTGCATCTCCTGTCTCCCCGCCTGCTTT







CTGGGCGCCAAAATCGGGCGGAGCGGTGGATCGGCGAGCGGCGGG







ACGGGGTGGGTGGGGACTGCGAGCGAGTGGGTAGGGGTGGGGGGA







GCGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PAQR6. In some embodiments, the sequences from gene PAQR6 comprise or consist of:











(SEQ ID NO: 46)



AGATTCCTCTTCTGGAGTTTATTTGGGAGCAGCTGGGATGATGGG







GACCCCACATCCATAGGGCTGGGAGGTCAGGGCAAGGGCAAGGGG







AAGAGGAAAGAAGGGTGCCTGGAGAGGAGCAGAACTGGGGTTGCC







GGCCAGGCCAGCAGAGCGACACCCTAGACCGGGCCGTAGAAGCGC







CGATAGGCCTCCTGAAAGCCGATGTGGTCAGCCAACTCGTCACAG







TCCGGATTGAGCTCACACACCTCCCTCCTGGGCTCCAGGGGATCC







GGGTAGGGGACTGGGGCTCTGAGACACCCGACGTGGTGGCATCAG







CCCCCGTGCAAAATGCCCATCCCTCCTCTCCCTACACATGGGGCT







CCAGGGTGCCCACCAGGGCAGGCTGCCTGTACCCCCACCACAACC







CAGAGAGGCCACTCCCTGCTGCAGCTCCCCCACAGACCATCCCGG







AGAGGCAGGGCCTTGGCCCAGCTCTGCCTTTTCTCTCACCCCAGC







CATT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PAQR6. In some embodiments, the sequences from gene PAQR6 comprise or consist of:











(SEQ ID NO: 47)



GTGCTGGGGCAGGGGCCACGGCTGTGGGCAGGAGGCCCTGAGCAC







CAGCCATGGCTACCATCTCTTCTGCGCGCTGCTCACTGGCTTCCT







CTTCGCCTCCCACCTGCCTGAAAGGCTGGCACCAGGACGCTTTGA







TTACATCGGTGAGGGCACGCCTGGCCCGGCCCGGGAAGAGGCAGG







GGCAGATGCCTTCCCAGAGCACAGAATGAACTGGGTAAATGGGTA







TTACAGCCCCAGGATGGAGGCAAATTATAGGAGGGACTTCCCTGT







CTTCCTATAACGAGAGCATCACTGGAAGGGTCCTCCACCTGCCAC







TTCTCAGGTAGCAAAACTAGTAGCATGTGTGCTGGGGCCACATGC







ACACATGCTCATTCATTCCTTTCAGTCACTCTGGGAGTCAGGGTT







TCTTATTCTCAATTTATGGGGGAGTAGCAGGCCCAGAGGATGGGG







TGACTTGCACTGGGAAAGGTGTGGCTCTGACCTGCCCATCCTCTC







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CADM3. In some embodiments, the sequences from gene CADM3 comprise or consist of:











(SEQ ID NO: 48)



AGTATCTCATGAGTTATCTTTCTCCGTGAAAACCATGGGCTAGAG







AAGGGGACTGCAGACACCGAGGGGAACTGTTCCTGTCATCCAGAG







GCTGAGAAACATGGTGGTCTGCTGTTTGAGGATGTAACAAGCCAT







GAGTTCCCCAACTGGTTCCAGTATTGCAGCTCTGATTTACAGCAT







GCATAATCTTACAGGGTTCTGGAGTGACTTCACCCCACAGGAGGA







ACTCTAACAGGAAGCTGAAGTCAGTGTTGCCCTAGGTATCTGACA







ATGAAGCTCACGAAATCCAAACTATTTTCTGAGTAGCTCTTAGGC







TACTATGTCTAATGACCCAGAAAAGTGATTGAATAACTTAAAGAC







TGCTTAAACTCAGGAGCAGTTATTTTTTCCCCACCCACCATACTA







GACCCCTTCCCCCAAAGAAGCTGGCCTGGGATTGGAAGGTAAACA







TGAAAGGTGCCAGTAAAATCCTAGCTTTCCATTCTCTTGATTTCC







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CADM3. In some embodiments, the sequences from gene CADM3 comprise or consist of:











(SEQ ID NO: 49)



TGAGACTCCCAAACCCCAGTGTCTCTACAGCATGCTTCCTTGCAA







ACAAACCTCCCTAGATGGGTCCCTGAAGCAGCTGGGAGCCAGGCA







AGTCTCCAAGCACTTTAGGAAAGTTCAGCCTGTGTTCCCCTGGCA







ATGGGATAAAATGTAAAAAGAAATGGGTTTTAATCCTTTGTGCTC







TACTTGCTGTTTGATCTAGGACAAGGTTCTTACTCTCTTTGAGTC







TGAATTTTCTCTTATAACAGAGAGATGATCATACCCCTTCTACTT







ACCTCCCAAGATGGTGTGAGGAAAACACAAGACATGCATGTGTTA







TAATTTCCAAAATAAGTACCCCAGTTGCTTACATGCTCTCATCTT







CATCCTATATTTCATCCTATATTTCAACTTCCTTCTTTGCGTCTC







TGAGACTCACACTATTTCTGTGGCCACCCAATTGTGCTGTAAGTG







GAGGGTCATATATCATGCCTGTGCTATGGCATTGCTCCTCACTGC







CATG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NDUFS2. In some embodiments, the sequences from gene NDUFS2 comprise or consist of:











(SEQ ID NO: 50)



GGTGCCCAGGGGTCTGGCTTCTCCAAACTCTCCTCCTGAGCCCTC







CTTTCCTGGATCTTTGTCTCTCCCTGCCTGTGTCTTCTGCAGCTT







CTCTGGCCTCTCCCTCTGTAGGACTCTGTCTGGGCCGCTTCTGTG







CCTGCCCTGTCTCTGCCTTCTTCCGCTGTGCCTTTGTCTCTGTCT







CTTTCCTCCTCTGTCTCTCTCGTGCATATGTGTCTGTGGGTCTCC







CTGTGGGTTCTCCCCAGCCTCTCTCCGCGCCAGCTCTTTTAAGCA







TGGTGAGCCTCTGCGATTGATTGGCTGATCTCTAAGCCACTCAGC







TGAGCTCTGTCGGTGGGACTCAGATGGGTGTATCATCGCTTCCCA







AAAAAATGGGACTTGCCCAGGGGAGGGGAATCTCTAGCAGCCGAA







TGGATAATAGGCACTGGGCCAGCTGATGACATAGCCTTTTCCCAA







ACCCCACAATCAGCCCTCAGCTGTCTCTTTCCCTTTTTGGTGGGA







CAGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NDUFS2. In some embodiments, the sequences from gene NDUFS2 comprise or consist of:











(SEQ ID NO: 51)



TAAGGAGGCATCAAGAGGGAAGAACAAATAAAAGAGGTAGCTTGA







CATGGCTCAGGATAGAACTGAGCAGCTGGGAAGAGTAAAAAAGGC







AGAACATTGGGGGAGGCAAGGAGACAGTGATCAGGGAGTTGACTG







CCCAAAGGTAGGATGATCCTGCTTGGAATGAACATAACAGAGGCG







GCAGATGACAAGGCAGGAGGGGTTAACGACTTTTCCTTTTATAGA







AGGCGGATCCAGGGTGGCACCTCCTCTTTTCTAACGTCCCTTCTC







TCCCTCAGGATCCCCCTTCCTGAGTTACCACTACCCACCCCCTCC







AAAGCCCAGAACAGCCATTCCGCCTCCATTTCCCCCCAGGGTTCC







CCCTCCCCACTTCCTGACTCCCCATTTCCCTGGCCCAGCTCCCAG







TAGCTTCCCCCAGGCTTTTGGCCCTGCTCCTACCCACTCCCAACT







GCCCTAAACCCTCCCCCAGCCTCAAGTCAGCCAGCCCCCAGCATA







GGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DHX9. In some embodiments, the sequences from gene DHX9 comprise or consist of:











(SEQ ID NO: 52)



GATCAAAATGATATTTAGAGCATGGTTCCCCCTTCCTCCCACTGT







TTTGTTCAATCCTGAATTCTCAGATATTTCTGGTGTCAGCCTTTT







TAAAACCGTCAGGTTGCAAAGGAGTGATACATTTGTTGATGATTT







GTTTTCTTTCATATTTTGTCTTTCTGAACGCATTGGGCTTGGTAG







CACTTGGTGACTTTAAGAACAATGAGATACCGAAGTGTGCCTGAA







GCTCATCGTTGCAGACATTTTTTGTTATCAGTATCTTATCATAGA







TTTCTGATACAAAGCAGTGAAGAATTCAGGAAAAGACAATGTGGG







AGGCACAGACAGTTGGGGGAGCCAGGACAGCATTGCTCTTTGTAT







GTCCTTTTATTTAAAGGCATAACAGACAGCAACACCAGAGGTCTG







TGTTCATACACACATGTGCTGCTGCTTAGGCTGAACTAAGCAATC







ACATGGGAAACTATTGTAAACTGATTTCTGGTGTCGCTGTTCCCC







CTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DHX9. In some embodiments, the sequences from gene DHX9 comprise or consist of:











(SEQ ID NO: 53)



TCAGTTCACACGAGGTATAGGAAGGAGTCTAACATTTATTTTTTT







CTTAAGCCTCACCTAGCCTTTTATTCACACCACCAAGTCTAGGTT







AGAATGTATCTGAGCAGTGAAAGGACCAGAATGCATAGTTATCAA







AGGGCAGTTTTTCCCTTGCTAAAACCTAAATTCATTTTTTAGACT







CTGCTAGCAGTTTTAGATATAATGTTACAACATGTGTTTTGTTCT







CCAGGACCTGATTTAAATAAATGTGATAAAAATAGGCTAAGTTTT







TTGTCATCCAGGGCTGCTTCCAACTGCAGGCAGATGTGAGAACTC







TGCCTCAGGAGCCACTGATATGCACAAAGTGTATGTGCATGTGTG







AGTTTGCGTATATTAGCTTAATAGATCGCAGTCATTAATGGGTGG







CCTATGCAAAGGAATCTTAAAATAATTATAACCGTAATGTAAAAC







ATTTGGGGCCTACTTTATTTCTTGGCTCCAAGCTAAGTGGAAAAT







TCCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TMEM9. In some embodiments, the sequences from gene TMEM9 comprise or consist of:











(SEQ ID NO: 54)



GTGAGGTCTGATTATTTGCCCTGTTTAAACAGGCGTGAACGCGGT







GCCCCAATCAGGTGGGACATTGGTCCAGTGGAACTTGGCTATTGG







CTGAGCGGGGCACCCAGCTTGAAAGTCCTGAGGTTCTTTTTTTCA







CTCTTCTTCACAGTCTAGGTAGGGCAGGGCTGAGATCAGAAGCCG







GCAATGGCCAGGCATTGTGGAATTAGGAAAAGCATTTGAGAGGGA







GTCAGCAAGTCTGGGTTTGAGTCACACCTCCACCATCTGGTAGCC







TTGGGATCACAGGTGATCTAGCCCAGCAGTTCTCAATCAGATGAT







TACAGCCCCCCCAGGGGGCATTTAGAGGTGGGTGAAGGTGCTTTT







GTGGTCCCAATAACAGGTGTATTACCAACACTGTTACAGGGACCA







TGGATGCTAAATGTCCCACCCAAAATCCCAGAGCACCCACATTGA







GAAACATTGAATTTGCCTCATTAAATCTGTCTTTTCTGTAAAAAC







AAAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TMEM9. In some embodiments, the sequences from gene TMEM9 comprise or consist of:











(SEQ ID NO: 55)



TGCACATCACCACACCCAGCTAATTTTTTAAATTTTTGGTAGAGA







CAGGGTCTCACTGTCTTGCCCAGGCTTGTCTCGAACTCCTGGCCT







CAAGCACTGCTCCAGCCTTGGCTTCCCGAAGTGCTGGGATTATAG







GTGTGAGCTCCATGCCTGGCCTAATGTAGCCCTTTCTGTTCCAGT







TTGTAGCATTTTTCTGCTTGTCCTTTGAAGAGTCTATAAATAGCA







TTGGAAAATAATTCTCATATTTAAAGGCACTGCTGGGTCCTCCAT







CAGGTTTCTCCTTTCGAGGCACATTTAGGGGGCGGTGGTCTTCAC







TGCTGTCCTTCCTGCCTATTCCAGGGAGCCCTGTGCTGTAGCTCC







TCTGTGTTCTCACCACTCAGGGAAAAAGCAGCTGTCTGTCCTTCA







CTCCCAGCCAGTGCCCTGTGCAGACCCAACTCCTTTCATCCACCT







GAGGCAGCCCCTCCTGCAACTGTCATTCTCCTTCTCTGAACTTGC







TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GUK1. In some embodiments, the sequences from gene GUK1 comprise or consist of:











(SEQ ID NO: 56)



TAAGTACTTCCCTAAGGGGCGGGGAAGAACGAGTGAGACATGGGC







GGGGCAGTCGCCTCATGACGTCAGTCTCGCGCGCGGGCGGAGGTG







GGCCGGTGCGGCGCTGTCACGTAGGTTCAGTGGGCGGAAGAGGTG







GCCCCGGATGCTGCGGCGCCCGCTGGCCGGGCTGGCTGCGGCCGC







CCTGGGCCGGGCCCCACCGGACGGTGAGTACGACAAGCGCGATCG







CGAGGGTGACTCGGGTCGAGGCGGCAGCGGTCCCTGCGCTTTGCG







GTCGCCCAGTCCACGCCGCCTCCGGATCTCCTCCTAGCCGAGACG







CCCTGTGCCGCATTCAACTGTGGGCCCTGAGAACGGGGGAGCTCT







GGAGGGCGGACGGGCTGGCAACGCAGCGGAGCCCGGGTCCCGGAG







GGCGGGGCGCTCCTCAGGAGCTGGGCAGGCCGGCAGTTATCGCAG







TGTCGAGCTGCTCCCCGGGCGTATAGGAGCGCTCGGTGTCTTCCC







GACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PDIA6. In some embodiments, the sequences from gene PDIA6 comprise or consist of:











(SEQ ID NO: 57)



GTAAGTAGAGGAAACTCCCAGAGTAGGAGCCTCCATCTGTTCACA







ACAGCCAAAAGGTGAAAACAACCCAAGTGTCCACTAACAGACGGA







TGGATAAACAAAATGAGGTCTAGTCATATGATGGAATGTGACTCT







GCTTTAAGAAGGAAGGAAATTGACACATGCCACAGCATGAATGGA







CCTTGAAGACGTTAGGCTAAGTAAAATCAATCACTCACAAAAGGA







CAGTACTATAGGATTCTACTTACATGAGATGTCTAGAGTAGTTAA







ATTTATAAAGACAGAAAGTAGGATGACAGTTGCCAGGGGTCAGGG







GGAGGGGGAATGAGGATTTGGCATTTAATGGGGATAGAGTTTCAG







TTTGGGGTGATAAAAAGCTCTGGAGATGAAGGTTGTACAACGATG







TGAGCGCATTTAATGCCATGGAACTGTACACTTAAAAATGGTTAA







AGGCCAGGTATGGTGGCTCACGCCTATAATCCCAGCACTTTGGGA







GGCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PDIA6. In some embodiments, the sequences from gene PDIA6 comprise or consist of:











(SEQ ID NO: 58)



CTAACCAAGCAAGCCAACAGGATTACCATAGGGTGTTGGAGAAGG







GAAAGTTGTAGGTAAGATTTAAATAAAGCAGTGTTTAATAGGCTC







AAAGCAAAACACAACTGTGTATAAAGGAATTACGGTCAGACCTGG







GCTACGAAGTGGATGGAGGTTCCTCTTTCCTTGGAAATGATGAAG







TTAAGACAAATGTGCAATGTTGTGTCCAGAAGGCCCTGGGTTGGA







AATCAAGGCAGCTTCTCAGTGTTAAGATGACCCAGGTCCTTCAGA







CAAAAGAAGGATGTTCCATTCTTACTCTATTTATCTCAAATAGCA







CAGTTTATAACAGTCTGTGCTTTTTCAGGAATAAGATTTTTCAGC







ACTATAACTTTTCGTTGATTAACAAAAGCAGTTTTAGCAGGTTCA







CAGATGTAAATACGTGGAGCCTGGCATATGGTGAACATTCGGTAA







TAGTTTCCCCATCCCCACCTCACTCATCTCCTCTTCTCTCTCACT







TTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SNX17. In some embodiments, the sequences from gene SNX17 comprise or consist of:











(SEQ ID NO: 59)



TCTCAAACTCCTGACCTCAGGTGATCCACCCACCTTGCCTCCCAA







AATGCTGAGATTACAGGCATGAGGCCTGTAGAAACTGATTTCTTT







GGGCCTCAGTTTCTACAACTGAAAGATGGATTCTTTGATCTGCAA







AGTCCCTTTAAGTCCTAAGATATTTTGTGAACAGACTTAGCTATT







AACTGTGCACTTTATAAAGCCCTTTCCTTCCTTTAAGGGTTCAGT







GAACAGTCTTGCTAGGTGGTCAAGGGGCTCCCTATAGGTCCTGGC







CCAGTACAAATCCTCCCCGTACTGTGGAGGGGCTGACTTGTACAC







AGTAAAATAGAGCACAACTTATATCTAGGACAGCTCCCTTGAGGT







GCTACCGCTGTCAAAGGAATTGTTTCTCAGATGACCAGGGTAGAC







AGGGGAACAGGGACTGAGGGAAGGGAGGGGCACCCAAGAGAACAT







TATGAGCATATGTAGATTGCCTCATCTCATTTTCTGTGTTTATGT







GAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SNX17. In some embodiments, the sequences from gene SNX17 comprise or consist of:











(SEQ ID NO: 60)



TTCGGAAGGAGTATGGGGCCAATGTGCTTCCTGCATTCCCCCCAA







AGAAGCTTTTCTCTCTGACTCCTGCTGAGGTAGAACAGAGGAGAG







AGCAGTTAGAGAAGTACATGCAAGCTGGTGAGTGGTTGCAGGAAA







CTAGGTTGACTATATTGAGGACTATGGGGAGAGACTTAAAAACAG







CTGGTTCTGTGGAAGGGCCTGATTTAATTTCTAGATCTAGGAAGG







CTCTTGTTTGATCTGTTATGCAGTTAGCAAAGTAAATAGTGTACG







AGGATGTGTAACTCTGTGTACTAGCAAAACAATTTGGGCACTAAC







ATCTATGAGAAATACTAGTATAACACTAGTCTTAATATCAGTGGA







GAGAACTTAATTTGTATCTGATGTGCTTTAGATTAACATCATTGT







TATTTCCCTAGTTCTATAGACTGGACTCACCTAGATGGCAGCCAA







GGGTGGGGCAGGTGAAGAATTTATGCAAACAGTGAGTGAGGTGAG







GCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DCTN1. In some embodiments, the sequences from gene DCTN1 comprise or consist of:











(SEQ ID NO: 61)



GTAGAGACCCACTCTGGCCGGGGGTTACCAGGCTGGTGGGAGTTG







GAGCTAAGGGAGCAGGGTGGAGGGGGAAGCCAGGACTGGAGATGG







GTGATGGGGGGATGAGGAAAGGGGAAGAGTAGGAATGGAGCCCAA







TTTTCAACCCTTTCCACTTGGGGCTGATGAGATTTTTTATGGTCC







CCACAGGAGCCATCCCTGGGCAGGCTCCAGGGTCTGTGCCAGGCC







CAGGGCTGGTGAAGGACTCACCACTGCTGCTTCAGCAGATCTCTG







CCATGAGGCTGCACATCTCCCAGCTCCAGCATGAGAACAGCATCC







TCAAGGTGAGGGAGCCACGGGGAGGACTGGGATGGGAGATGGAGC







ATATTTGCCCCATTGATGATAGATGACAGGGGCCTCCTAGCACCT







CTTACCACTATCCTTCTTTTTTCATCCACAGGGAGCCCAGATGAA







GGCATCCTTGGCATCCCTGCCCCCTCTGCATGTTGCAAAGCTATC







CCAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DCTN1. In some embodiments, the sequences from gene DCTN1 comprise or consist of:











(SEQ ID NO: 62)



AACATAGTTCTGAATTTATGAAACTTCCCACCAAAAAAGAAGGTC







TTTTGCCTCAGAGCAATGGGGAATCCTTAGGAGCAAGGGAGTGTG







TTTGCATCCCCAACTTAAGCTTTGCTGCTGGTGAGCACTAAGGGT







GCAGACATATTGGGGTCTCTGGAGCATTCTCCGTGCCCTTCTTGG







AGATGGCATCTGAGACAGGGAGGCAGGAACAGCCTCATGTTCCAA







GTACTTTGTACAGTGCACACTCTCTTTGCCACTGTTCTTCACACA







TTGTACCCTGTCTTGTGGCCTCACTCCTGTTTGGTGTCTTTCTTT







AACACTTATCTGCTCGTCCCCAAAGATAGGTGGCAGAGAGTCTTG







TTAGAGAAAGTATGATGATTAGGAAAAGGGTTTTCCTGCAGGTTG







TGTCCAGGATTCCTCTGGTGTGTCCCCGTAACCCCCAAATCACCT







AACTCCTCTCTCCTCTTTTCCTGCTCCCCCATGGGCTATCCCGAG







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene WDR54. In some embodiments, the sequences from gene WDR54 comprise or consist of:











(SEQ ID NO: 63)



GCTCGCCCCGCCCACAGTTGCCCCGGCAACCGCGCCGCCCGCGGC







ACGTTCCGGCCGCTCACCCCGCCCAAGGGCCGTGCGTACGTGCGT







CGTCTCTATGGTGGCGGCGGATTTGGAGGGACCCTACGAACCAGG







AGTCAGGCGAGCCGATCTGGGGCTGCAGGTGTTACCTCTGATCTA







GGCCGGGGGCTTCAGGGATCCGAGCCGAGGGAGAAAGCCTTGGGG







GCTTCATCACACTTATTTGGCTCGCGGGCTTGGGGCCATCGGGTG







GTCCTGTGTCATCCCCATTCCTCCTCTGCGATTCCCCCGTCGCCC







CAAAACAGCCCTGGGACTCCCCTGTCTTGCTCCTCGAGACCCTCC







CCCATCACCGTCCTCTGGGCACCCCATCCTATGACCCAGTCTCAG







GCATCTTCCGAAATGCTCGATGATAGCCGCCCTCGGGCATCTCCG







CTGCGTCTGTTTGTAGCGCGCCTGGTGATTCGGCTGCACCCCCAC







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene WDR54. In some embodiments, the sequences from gene WDR54 comprise or consist of:











(SEQ ID NO: 64)



TGAGGCATGGAGCTGGGAGTGATGTTGCTGAACCCAGTCCTACCC







CCAGCCTTTTCTCTCCAAACCTTCAGGAGCAGGCATGTCCTAACC







TCAGAATCTCCCCAGGCATGTCCTAACCTCAGAATCTCCCCTCTC







CTGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAACACTTTGGGA







GGCTGAGACGGGCACTTGAGGCCAGGAGTTCGAGACCAGCCTGGC







CAACATGGTGAAACCTCTTCTCTACTAAGAATACAAAAAAAATTA







ACCGGGTGTGGTGGCGTGCACCTGTAATCCCAGCTACTTGGGTGG







CTGAGGCAGGAGAATCGCTTGAACCCAGGAGGCTGAGGTTGCAGT







GAGCCGAGATGGCGCCACTGCACTCCAGCCTGGGCCACAGAGCAA







GACTCCGTCCCCCAAAAAAAAAAAAAAAACAAACAAACTCATCTT







CCCTGCTTTGATTTTCTCCCTACACTGATGCACCTCCCTCCAGGT







CCAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CAPG. In some embodiments, the sequences from gene CAPG comprise or consist of:











(SEQ ID NO: 65)



GTAGGAGGAAGAAAGGAAAGTAGGAGTGTTGAAAGGTGAAGGTGG







CAGGGTGGCTGCCAGCGCGGCTGGCTGGTACCCTCCTGGGTGGGC







AGGCGAGGGCTGGCTGTGGTGGCCTGGGAACAGAGACCCACATTT







CTGGATACCACATTGTGCCAGGGTGGAGTTCCCCCAGCCTGCTGT







CCTGCCAGCCTTATGCCAGGCTCCAGGCACCTGATCTGATGGCAG







CTGTGAAGCTGCCCGGGAGGATGGGTGGGCTGTGCCCATGCTGCA







CATGGAGGAACTCTTGGGTCAGGGCTTGTCCCAGCTGTGCCAGAC







CTCCTGACGGAGGCCACCACACTGCTCCTGAAGGCCCTCAGCCAG







ACTGGAGGTGAAATCAGATTAGAAGCTGCCTGTCCCCAAGACAGG







ACTGTGGTGTGATGCTATTTGGGTGGGAGTGGCTTGGGGAGGGGG







CAGGGCAGTCCTAGCCATTTGAATTCAAATGCAAGTGGCACATTT







GATT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CAPG. In some embodiments, the sequences from gene CAPG comprise or consist of:











(SEQ ID NO: 66)



AGAATGTTTTATGGCCTCAGCCATTCCCTGAGAACTGAGCTTAGC







CAGGTGCAGTAGCCCACGCCTGTAATCCCAGCACTTTGGGAGGCC







AAAGTGGGAGGATTGCTTCAGTTCAGGAGTTCTAGACCAGCCTGA







GTAGCAAGACTCTGTCTCCAGAAAAAATAAAAAAATTAGTCGGGC







ATGTTGGTGCAGGCCTGTGGTGTCAGCTGCTCAGGAGGCTGAGGC







AGGAGGATCGCTTGAGCCCAGGAGTTCAAGGCTGCGGTTAGCGGT







GATAATGCCACGGCACTCCAGCTTGCGTGACAGTGAACCCTGTCT







CAGGAAAAAAAAAAAAACAAAAAAAACCAAAAAACTGATCTTAAA







AAAAATCAAATACTTGCATTCAGGGCAAGGAGAAAGACAAACTGA







GGGAGGAGGAAAAAGTAATGTGAAGCCAGTGACCCTGCCCAGAAA







ATGTGACTAACCCAGCAGCGGTGGGGGCTGTGCCGCGCCTGCCCC







AGGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DBI. In some embodiments, the sequences from gene DBI comprise or consist of:











(SEQ ID NO: 67)



CAGCAAAACTAAGCACCCTACTTCCGTTGTCCCCACCTGTTCCCG







GCGTCCCCTTCGGCTACTCCCGGCGTTTGCGCAAGCGGTCCCACG







TGGGCTCGGGCGGGGCTAGCGCCGCGGCGGGGGCTGGGCACGCCC







CTAGCGCATAGCTGGCTTCTGATTGGCTTTCCGGTGCTCGCCCGA







GCAGGGTTGGGGCGAGTGGACCGCGCCTCTAAAGGCGCTTGCCAG







TGCAATCTGGGCGATCGCTTCCTGGTCCTCGCCTCCTCCGCTGTC







TCCCTGGAGTTCTTGCAAGTCGGCCAGGATGTCTCAGGTACAGCG







CGTGCACAGCCAGGCTGCGAAGGTGCAGCGGGCGGGAGGCCCGTT







GGGGGCTCAGCCGGCTGCCAGAAGCTCTCGGGCTCTTTCCTTCCG







TGCCCCTCACTTGCTCATGGGCCCATGCCTAGCCCTGATTCGTTG







GACAGAGCCTTGTGAGCGGGATTTTCCGTTTGGGGATTTCTAAAT







CTGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DBI. In some embodiments, the sequences from gene DBI comprise or consist of:











(SEQ ID NO: 68)



ACAGGGCAGGACGTCGCGGCGGAGTGGGGAAGCGAGGAGTCCGTG







GCCGAGAGCTTGGAGGTCAGGGGAAGTACGGGGCCGGCTGCTCAG







AGTGCGGGACGAGGAGAATCGCGGCCCGGGGAGAGGTGACCCAGG







GGCCCCTCCCTTCTCTCCAGTGTAGACCCTTGTCTGAGACCGAGC







TATGTGGGGCGACCTCTGGCTCCTCCCGCCTGCCTCTGCCAATCC







GGGCACTGGGACAGAGGTCGGTGTTGAACGCGCGGGCCCCAGGGG







GAGGGAGGGGACCAACGGGCTCCGGCGCTGACACCGCGGCACTCA







TGCCCTGTCCCCTTTCAGCTGTTTCCAGCATACTGTGCCCCGTCT







GTCCTCAGGCCAGGGCTTCGCTGCAGCCCCGGCCACTCCCTAGTG







CCTGGCCCGGTGGTGGCCAGGCAGTTGGCCGCGCTGCTTCTCCCG







CAGAGGGGACCCCCACTGGGGGCGAAGGCTTGGCCTGCCCTCTTC







ACTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene BIN1. In some embodiments, the sequences from gene BIN1 comprise or consist of:











(SEQ ID NO: 69)



GTAAGTGCCCACCTCCAGCCCCTGTCTGGCTTGTCCCCAGTCTCT







AGGGGTGCAGCATGGAAGGAGAGCCCCGAGGAGGGGTTGCAGGAG







GGACCAGGCCACCATGGATGTGAGGGTGAGGACAGGGTCCTGAGC







TAGGCTGCCCCAGCACGGGCTTGTCACCAAGGCTGCCAGGGATGA







GTGAGCGCAGCTGGGCGCATCAGCCCCTCCTGCTTGCCCACCCCA







GCCCAGCCTCCCAGCGCAGGAGGAGCATTTAGAGCACCTACTGGT







TTATGCCAGGCACTTTCCAGAATCTTCTCATTTAATCTTCGTCAT







CACCTTTACAGCCAGACACTTCCTCCTTTTACAGATGAGGAGATG







GGGGCTAGGAGGGTTGAGTAACTTGGTTCCAGCACTCAGAAGTGG







CCAAGTCTGAGTGTCGGCCCAGGTCAGCCCAGCTCTGGGGTTCCT







GCAGGGCCTGCCGTGGTGCCCGTGCTGCGGTGCCCGTGCTGCGGT







GCCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene BIN1. In some embodiments, the sequences from gene BIN1 comprise or consist of:











(SEQ ID NO: 70)



AGAGGCTCAGGGAAGGCTCAGTACTTGCCCAAGAACTGGTCATGG







TAGAGCCAGAATTCCAACAGGGTCTCCTGGGCTCTGTCCCTGAGA







CCCCCTGATACAGGCAGAGATGCTGGGAGGGGCAGGCGGGTGTGC







AGGCGCCCTTGGGGCATGCGCTGGCAGCCCAGGCTCCTGGGAGCT







CTGGAGGCTCCACCGCAGGATTTCCCTCTGGAGGAAGCCAGAAAG







AGCCAGCCTGGTGCGAGCTGGTAGGGCCATTTTGACAAGTGGATT







TCGGTAGGTGCTGAGCTTGGGCAGCACAGTCACACCTGCCTGTCC







CTTTGACAGTGGTAGGAGAGAGGATGTGGGAGGCGGGTGGCTGGC







CGGGCTCCGCTGGTACCCACCCTGCCCCCACCAACCCCAGCCGCT







GGTGACATTTTCTCTTGTCTTGTGATCCTGCCCATTGCCTTTCCA







CCCCGGCCTCCCCGCCCCCTCCCTGTTCTCTCCTCGTGGCCTGTT







ACCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene KIF5C. In some embodiments, the sequences from gene KIF5C comprise or consist of:











(SEQ ID NO: 71)



ACTGCTTTCTTTTCTCTCTGGTAGGTCAGCAAAACTGGTGCCGAG







GGAGCTGTTCTTGACGAAGCTAAAAATATCAATAAGTCTTTGTCT







GCTCTTGGAAATGTGATCTCTGCTTTGGCAGAAGGGACAGTAAGT







GATCCTGCCCCCATCTATTAAGTAATATTATGAGAAACCACCTTT







TGGGGCCTCATAGTCCCTTTGCCACACACCCCAAAGGCTGGTTTG







TTTTTCTGTTTGGAAGAGGACAGAGGGAATCCTGGCTATGGATGC







CTGCTTGCTGGGAGTTCCCATCTACTAAAAAGTATGAATGGTGAT







GCTAATTTCCTATGACATAGGTCAAAGAATGGGCATTCTAAACTT







TGGCAAGATCCAAAGACCCAGCCACAAATTCTTGTTTTACTCGGT







TTCTAAGCTCAGTGTCTTCATCCCTGACTTGCTTGCCTCTGTGTT







TTTCTCAGAATGGGCTGTCAAAACCAATACTTTTTCTTTTCCTAA







ATAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene KIF5C. In some embodiments, the sequences from gene KIF5C comprise or consist of:











(SEQ ID NO: 72)



TACGTGTGGTCTCTCAGGACCCATCCTCTGTGCTAGGTCTTGGGT







CTTAAGAGTGAATGGCAAGGCTGGGTGCAATGGCTCACACCTGTA







ATCCCAGCACTTTGGGAGGCCAAGGCGGGTGGATTGCCTGAGGTC







AGGAGTTTGAGACCAGCCTGGCCCACATGGTGAAACCCTGTCTCT







ACTAAAAATACAAAAATTAGCCGGCCGTGGTGGCATGCGCCTGTG







GTCCCAGCTACTTGGGAGGCTGAGTCAGGAGAATTGCTTGAACTC







AGGAGGTGGAGGTTGCAGTGAGCCAAGATCATACCACTGCACTCC







AGCCTGGGCGACAGAGCGAGACTGTCTCAGGGACAAACAGCCTTT







TACTAAATTACATCTAGCAGGTGATATGTAAACTTGAGGCATCAC







TTATTGAAGCCAAAGCAATAATTATATTTTAAAAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DYNC1I2. In some embodiments, the sequences from gene DYNC1I2 comprise or consist of:











(SEQ ID NO: 73)



AGCCCACTGTTTTTACTGCAGCGTGTATAAACATTTAGACATAAT







TTTAAGGTTCAATGAATGTGACTATCCTAAAAAACACAGACTTTT







AATTAAATACTTATTTCTTTAGTTCCTATATAACTAGGAACTTGC







CTTCTTTTCAGTAGACTGTTAAAAGAAGGGCTTTCAGCTCAACAT







AAACTGTATTAGTCACTTAATATCATACACTGAAAAATTATTTTG







AAAAGATACTTGGGATTGGGGACATTTATGATAAAATTTAACCAG







AAGATGGCAGTGATGATCCTCCTATACTCCCTGCAACGGTAATCA







AAAAGTTTTACAAATGTCATAGCATGAAATGTGCACGTTCAGCTG







TTGTTATATAATGTTAAAAGTTGAGGATCTAGGGGAAAGCACTTG







CTGTTTATTTTATATACTATGAAAAATGTGTATTTTTCTATAAGA







AGGGATTAAGAATATTTTGGGTGTCTGTATCTTTGTCTGTACACT







TCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DYNC1I2. In some embodiments, the sequences from gene DYNC1I2 comprise or consist of:











(SEQ ID NO: 74)



TAAGGAAGTTCCCATAAGTCTTGCCTTGTTTATTTGCATGCATCA







CTTTATGTTATGCATAATGTCTAGAAGGCATGCTGTTTTATTGCC







TGTTTGCATTTTTCACCCAAATTGCTGGAAACTAATGAACATTTT







AAAGGAGCCATTCCTTTAGCTGCAGTCATTTCTCTAAATTGATAT







CTTTTTTTGGGGGGGAGGCAGTGAAATCATTTTAAACATTACTTT







TTGAAAGAAAAAATTATTAGACAAAAGTTAATATTTTAAAATAAA







TCTTGAGAAACTAGAGATTAAATAACTTAATGAAACATATTTCAG







AGTTGAATTTTGGCAGCAATTGTAAATAATATTTATAATCTGGTG







ATATTAAAAGTGAAAAGTTACAAGGGGGAGAAGGAATGCTCTCCT







TTGAAAATTAGTGGCCACATTTGGTGATGGATGAGATGAAGATGC







ATCCTTCCATAATCTTGCCTTTTTAAAAAAATTATAAAGCTCAAA







TGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene FN1. In some embodiments, the sequences from gene FN1 comprise or consist of:











(SEQ ID NO: 75)



GGGGATTTTGGTTTTGGTTTTGGGTTTTGTTTTTTTGCGGTGGGG







GGGCTGGTATTTGGAAGAATTTAGCTCTTTATGTTACAGAAATCT







TTTTTGCAAGGACTTAGAAATGATAATGCTTAAGATTGTTCTTGC







CCAATGTGGGAAGAGAATCTAAGGTTTTTATATGTCTTGCAACCT







CATCAAAGGAAAATTACTGGCATCATTTTCATAATTTGAAAAAAA







AAGCCAAATTAATATATTTCTTTTTTGATTCACTTTTTAAGTGAT







CATTTTTAAAACTTTACTTTTGACCCACTGAATTTATTTAGATAG







AAGGAAAAGAGATGATGGGAGGGAAGTTTAGATAAAGGATGGAAG







TTGGTTTTATTTAAACAATAGCCCTGTGATTTCCTAATGAGAAGT







GACTAGAAATTGAAGAAACCAAATAAGGAGGATATTGGTCAATTT







AGCTTTAGTTTCTCTTACTCTCTCAAGCCTGCCCTGTTTAACTCC







AAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene FN1. In some embodiments, the sequences from gene FN1 comprise or consist of:











(SEQ ID NO: 76)



ATACAATAAGTAAATGAAGTCTGTGTTCTGTATTACATTATTTTT







GTAGGATCGTTTCACAATTTTCTCACAATTCTTACTTTGATAGTA







GAGAAAATAAAAGCAAGCCAAGAGACTTTTTTTTAAAAATATATT







TTATTTGCTATAAACAACTATGATTTGCATTTCTCATGTGAAGAA







TAATAATTATCGGCTAAAAATGGATTTGCTGCATTGATTTTCAAC







GTAAATATTTAAAAGATTAATGCCAGATAATTTTATTATACTCAC







ATTTAACGACAAGCAAAGCTGTTTATAATGATGACTGTCCATGTA







CACAGTTTTAAGTTGAAGTGAGTGAATATTCAAGATAATTAAATG







CTACATTTTCATTTTCAGGCTCAAGTGGTCCTGTCGAAGTATTTA







TCACTGAGACTCCGAGTCAGCCCAACTCCCACCCCATCCAGTGGA







ATGCACCACAGCCATCTCACATTTCCAAGTACATTCTCAGGTGGA







GACC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SERPINE2. In some embodiments, the sequences from gene SERPINE2 comprise or consist of:











(SEQ ID NO: 77)



GTGAGTGGCCGCAGTATGTCATTTGATTGCATAGTTTGTGAATGA







AGCATGAGTTAGACTCCAGGGTTCAAAGTGGTGTCTGCAAGTCCA







ATTCAATGGCCTGTGGGTTGAGAAACTCCTGGACCAAGGAAGCTC







TATTCAGTCTCCCTCCTTCCCCTTCAATCTGCCATTGCTCACTGT







GCTCCTGGGAGGTGTTTCTCCTGTAGTTTGGGCAACAGAACAGAA







TCCAGCCCCTCCACTGTCTCCTCTCCTTGCCATATCTGGTCTCCA







GCATCCTTGCCCACAGGCTCTTGTTGATTTTGAAGGTTTTCTTGC







TCATTCCTTCCTGCTTTTGCATCTGCCTCTCCCTTTACCTGGAAT







GTCCTCCTCATCCTTCCAGGTTGATTGATCTCTTTGCCTTCCATG







CCTCTTGCTTCTGCCATTGCCAAAGACATAGGTACCCTGTTGTCC







GTTATGATTGGCACATGATTACAACTTTTACACCCCCCCGATTAC







AGCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SERPINE2. In some embodiments, the sequences from gene SERPINE2 comprise or consist of:











(SEQ ID NO: 78)



GCTTCAGTGGTTAGACTTTAACCCCACTCCCTCCTTTTCTTAAGAA







ATAGGCCCCTGAGAGAACAGAAGACTTAGTTTCTTGCTACTCATA







GCTATCCTGTTTATACTGTTCTGTTATTTTTATTTTTGAGACAGG







GTCTTGCTTAGTCACCCAGGCTGTATGCAGGGGCCCGATCATGGC







TTACTGCAGCCTCGACTTCCCAGGCTCCAGCGATCCTCCCACCTC







AGCCTCCTCAAGTAGTTGGTATCACAGGCGTGCCACCACACCCAG







TTAATTTTGTATTTTTGGTAGAGACAGGGTTTTGTCATATTACCC







AGGTTGTTCTTAAACTCCTGAGCTCAAGCAATCCACCCACCTCAG







CCTCTCAAATTGCTGGGATTACAGGTGTGAGCCACTGCACCTGGC







CTATACTGTTCTTACTTGCTGCAACATAAAAGATTTTAAAAACTT







TTCTAGATTCTTATGGAAGTATTGCTGTATCTTCTGATTTCTCTT







TTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PPP1R7. In some embodiments, the sequences from gene PPP1R7 comprise or consist of:











(SEQ ID NO: 79)



GCCGGCTGCGGTGAAGAGAGCGCAGGGTCCCGAGGCGACCCGAGG







CGCGGGGCCTCGGCGCCCACCGGGCAGGCTACACTGAAGGGCACC







CCGAAGACGCTGGGGGCGCGGGGCCCAGGGCCAACGGCCGCAACC







CCGCCGCGATTTGGGCACGGAGAGGACGCGGGCTCGGCGGTGCGC







GAAGGGCGACGGGCGCAGGGGCGGGAGACCCGGGGTCGGGCTGGT







CTAAGAGTCGGGCGGCGGATCCTCCTGGGCCAGGGGCGCGGAGCC







GCGCAGGCCCCACAGCCGCCCTGGGGAGATGGCGGAGCCCGGCCC







GCTCTCCCGAGCGCAGGTATCTCACCTGGATCAGGCGAGGGTCAC







GCCAAGCCGGCTACACACCACGGAAAGGAGCCCTTCCGCGCTTTT







ATCCCACCGACCAATCGCACAGCGACTAGCACCGATTGACAAGCT







CCACGGACCAGCCACTTGCGCGTATGGGCGGGACTAAGCTAGCCA







GAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PPP1R7. In some embodiments, the sequences from gene PPP1R7 comprise or consist of:











(SEQ ID NO: 80)



GCCCCCGGGCCGGGCAGATGCGGTTTCCTCCCGACTCGCGATCAA







AATGGGTGAGTAGCGCAGAGCTCGCCTCTTGGAGGCCTCTTTCTG







AAGGCGGAGGACGCGGGGCGGCTCGTTCATGGGCCGGGTGGCTCC







GCAGGGTAGACGAAGGCTGCAGCGTCCGCACTGGGCTGGGAACGA







CTTGCAAGGGGAGCCCAGCTGGCGCCCCGGAGCCAGCCAGCTGGC







TAGCGGCCCCACCAGCGCAAGTGCCCCGCACCTGCCCGCCTGCTC







CGAGCGTCAAGAGAAGGCGGCATTTCGCATGGGTAAAATCCGCGC







GGGGACGCCGGGAGAATGTTGTTGCTCTTGCCGTTCGGCCCATTG







TACAGACGCAGCCAGCGAGGCTCGCAGAGGTCAGGGATGCACGTA







GGACTGGCCCGCACCTTGCAGCCACGGAAAGATCCGCCTGGCCCG







CGGCCCCTCCACGCCTCCGAGGTCGAGACAGTGACATAAGTCAAC







TCTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene STK25. In some embodiments, the sequences from gene STK25 comprise or consist of:











(SEQ ID NO: 81)



GTAAGGAAACTGAGGCAGGAGAGGCGAAGCAACTCGCCCAAGGAA







TGGGCTGTGCCGGTAGCTTCTCCAAGGCCTGGTGCGCCCTGGCCT







GTGCGCGTGATCCCGGGCGGGGTCTCAGTACGAACAGGGGCTTCG







CGATGTGGCCAGCGCGGGCTTATCTCCGTGCCGTGACGACAAGGC







TAGAAGCCTGGGCGGCCGCGGTGGATCCTCGTGGCGAGGCGGGGA







GGGCGCCGGGCCTCACTGGCCCGCTCGAACCAGGGCTGCGCAGGG







CAGAGCCCAAGGGAGAGGGCCGCGCGGCCGCCGTAACGTCGGAGA







CGACGCCTGGCAGGCGCCAGCAAACGCAGAAGACCCGCGAGGGCG







AGGCAGGGAGGGCGCGACGCGACAACGACCACGTGCCCCGCCCGC







CCAGGCGCGCGGCCTGCCGGGAGACGCAGTCCAAACCTTTCGGGA







GCAGGACTAGGGGTCCCAGAGAGCCTCGCGGCGCGCATGCGAGCC







CCGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene STK25. In some embodiments, the sequences from gene STK25 comprise or consist of:











(SEQ ID NO: 82)



TTTCACCATGTTGGCTAGGCTGGCCCCGAACTCCCAACCTCAAGT







GATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTG







AGCCACTGCTCCCAGCCTCTTCTAGGCACTTCGGAAAAGTGCAGC







GATTACTGACCACACGAAGGCCAGAGATGCAGCAGGCATCTATTT







ACATGCATTAAATACATATATTATCTTCATGCATTTACACATTCA







TTCAACAAACATTACTGAGCGCCTGCCGTGGGCTGGCATGGTCCT







GGGCACAGCTCCGCTTGCAGGAAGCCCACTTTCTCCTGGGGAGTC







AAAGCACAAACAAAGCCAAGTCAGTACGTAAAGAAGCAGGGGTGA







TGAGAGATGCGGACCCAGGAGGGGTTCAAGGGGCTGTCCTGGACG







CATGACAGGGAAGCATAGCTTCTTAAAGGACTCGGCAGTGCACCA







TGAGAAGACACGCACCAGGGAGCAGAGAGAAACGCCAGAACCCTC







TCGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MOBP. In some embodiments, the sequences from gene MOBP comprise or consist of:











(SEQ ID NO: 83)



GTTCAATGGTATGTCTGTGAGATCAAAATGTGTTGATGTATGTCA







GCAGGTCAGCAGTTTGTTCTTTTTCCTTGTGTAGTATTTTATTGC







ATGAATGATGATTGTTTTAATAATAAATAATAATAAATTTTTTAT







TTTGCTTTTTAGAGAAAAGGCTTTTCCTAACTCTAGAATATTATT







TAGTCAAGGATATGTTTGGTTTTAAATGTTTTCCTATCTTACATG







GGTCTCTAAAACATAAGGAAAACAAGAGCCCATGTTTCTTTTTCT







CCTTAAAAACACGATTTTTAAAAATACACAAATGAAAATTAATGA







ACTGTTAGTTGAGTTCCTTTCCACAGAGTTGGGGGGAAGTTGGTC







TTCCAGGGTGTTGCTCTGTAGGGCCCCCCTGAATGTTACCTTGGA







TTATGGGAGTAGCAGGGGGCTTCCAGAGTAGAGGGCTCCCTTTCC







TGATGTGCGTTTATGTCTCCTCCTGTCTCCTTGCATCGGCGATTT







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MOBP. In some embodiments, the sequences from gene MOBP comprise or consist of:











(SEQ ID NO: 84)



TAAGCGGCCGCCCAGCCCCGCGGCACCAGTTGGGCACAGCGCGTG







GTCTCGGCTCCCAGCACGCCCCTCCCGCTCCGCACCCCACTCTTC







CCCCTAGTCGGCTCCGGGTTAGGCTCCGACACCGGAAGGCACTCC







AGGGAGACTGGAAGGTGGGTGGGGGAGCAGGGCCTTCCTACCTGT







TTCCAGCTCCTGCCAGCGTCGCTTAAGCAGCAGAGGAGAGCCCTG







GCTCCCGCCTCCAGCTTCTTTTGGCCCTCTCAGAACCAGCCGCCG







TGCCAAGTCCCCTCAGAGGCCCAAGCAACAGCCAGCTGCGCCCCC







CGCGGTGGTCAGAGCGCCAGCCAAGCCACGGTCCCCTCCGAGGTC







TGAGCGTCAGCCACGGTCCCCTCCGAGGTCTGAGCGTCAGCCACG







GTCCCCTCCGAGGTCTGAGCGTCAGCCACGGTCCCCTCCGAGGTC







TGAGCGTCAGCCACGTCCCCGCCCAGAGGTCCGACCACCGCCAGC







CAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene HHATL. In some embodiments, the sequences from gene HHATL comprise or consist of:











(SEQ ID NO: 85)



GCAGGGACTGGGATGTGACAGGCTGGAGCTCAGGGACTTGTTGGG







GGAATGGGGGTCAGTGACAGGCTGGAGGCTTAATGCGAGGATGGA







GGCTTGGCTCTAGGGGCAGCACCTGGGGTGGTCATGGGATCTCAG







GGAGGGGAGTGGGAGTCAGAGAAAGGGTCTTAGAGAAGCTGGGGC







TTTGTGAGGAGCTAAGGGAGACTCACTGATCGAGGTGGGGACCCT







GCAGGCAGAAACCTGACTGTAGTGATAGAGGTGGGGTTTCTCCGC







ACGCAGGTCATGGGGGCCTTGGCAGGAGCGGTTTTGGCAGAGGGT







GGGGCCGGTGCCTCTGGAAGGTATGAAGATGTAAATGAAGGTGAG







GGAGGCAGGCTGCGAGTGAGGGCTCTGCGGGCAAGAGCCTTGATG







GGTGGGGTGGGGGCAAGAGCAGCAGCTGGGATGGGGCACTGGTGT







TCGGTGGCCTGTGAACTGTGGGGGGCACAGGGGCAGGCAGAGTGA







TGGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene HHATL. In some embodiments, the sequences from gene HHATL comprise or consist of:











(SEQ ID NO: 86)



ACCTTGACAGGTCCCTGCCCCTATCTGGCCCATGACCCCTCCATC







TGTACGAGGAGACCGTCGGCTGCTGGGGGGCCCAGGGGAGATGGC







TACAGGGCTGGGGATGAAGATTTAGGGGAGGATCAAGGGTGGGCA







AAAGGGCCACATCAGCCACCTCTTGGCCCCGTCAGCCCAAGAGTT







GCCTGGACATGTTCCCTGCTTCCTCCCTTTAATTTGGGTCCTATG







TCTGTATCCATGGAGTCCTTGGGAAGCCCCTGAGCCAGGCTGAAG







CGGGATAGGAAGGGTAAAGGTGCTAGCTGAGCCGTAGTACGGCCC







AGCAGTTCTCCAGGGGACAAGCTAGGCCCCCGCTGGAGGGAGTGG







TGAGACTCAGCCGGGGCCTGATTCTGGTCAGCACCTTGGACAGCA







GCCAGTGCCCTCCCTGGCCCCGCCTGGCCCCGCCTGGCCCCACAT







CCGCTACTCCTGGGCACCTCCTCAAATAGCACAGCCTCCAGCTGG







TGCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SHISA5. In some embodiments, the sequences from gene SHISA5 comprise or consist of:











(SEQ ID NO: 87)



GAGGAGCAGCCGCGCCCTACCCCGCCAGCCAGCCTCCTTACAACC







CGGCCTACATGGATGCCCCGAAGGCGGCCCTCTGAGCATTCCCTG







GCCTCTCTGGCTGCCACTTGGTTATGTTGTGTGTGTGCGTGAGTG







GTGTGCAGGCGCGGTTCCTTACGCCCCATGTGTGCTGTGTGTGTC







CAGGCACGGTTCCTTACGCCCCATGTGTGCTGTGTGTGTCCTGCC







TGTATATGTGGCTTCCTCTGATGCTGACAAGGTGGGGAACAATCC







TTGCCAGAGTGGGCTGGGACCAGACTTTGTTCTCTTCCTCACCTG







AAATTATGCTTCCTAAAATCTCAAGCCAAACTCAAAGAATGGGGT







GGTGGGGGGCACCCTGTGAGGTGGCCCCTGAGAGGTGGGGGCCTC







TCCAGGGCACATCTGGAGTTCTTCTCCAGCTTACCCTAGGGTGAC







CAAGTAGGGCCTGTCACACCAGGGTGGCGCAGCTTTCTGTGTGAT







GCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SHISA5. In some embodiments, the sequences from gene SHISA5 comprise or consist of:











(SEQ ID NO: 88)



GTCTAGCTCTTTGGCAGGTTCGGAGCGACCTTGGCCGTTGGCCTG







ACCATCTTTGTGCTGTCTGTCGTCACTATCATCATCTGCTTCACC







TGCTCCTGCTGCTGCCTTTACAAGACGTGCCGCCGACCACGTCGT







AAGCGTGCCCACCCTGCCCCACTGTGACCCCCTGGGGTGGTAAGG







GAAGCTGACCAGGCCCTGCTTTGGCTAAGCTGGAGGGATGGGTGG







CAAGCCTGGCTCTCAAGACCTGGGGAGATGGAGGAGGGTCCTGAC







TGGAATTCTCTTTGCAGCGGTTGTCACCACCACCACATCCACCAC







TGTGGTGCATGCCCCTTATCCTCAGCCTCCAAGTGTGCCGCCCAG







CTACCCTGGACCAAGCTACCAGGGCTACCACACCATGCCGCCTCA







GCCAGGGATGCCAGCAGCACCCTACCCAATGCAGTACCCACCACC







TTACCCAGCCCAGCCCATGGGCCCACCGGCCTACCACGAGACCCT







GGCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GNAI2. In some embodiments, the sequences from gene GNAI2 comprise or consist of:











(SEQ ID NO: 89)



ACACAGGATTAGCCAGCGACCTAATCCGCCAAGCTATGCAAAAGG







AGGCCTCTGCTGACCCCTGGCGGTCACCAGCCGGAGTCTGGCTGC







GCGGTGACCCACCACACGTTGCCCAGCCGTGTGGCTGGGATGGTC







GGTACCCATGGCCGTGCGGTCGAGCCTTGATGCTCCAGGACAGTC







CTGGAAGGCTTCCTGGCAGATCCGTGGGAAGAAAGCCGACAGAAG







GGCGGGAGGCGGGAGGGGAAAGGTCCCCCGGGATGGGTAGGGCCT







CAACGCTGGGCATCGGCGCAGGTGTGTACTGCAGGGAGCCAGCCA







GACCCCCCACGGACTGCCTGACCTTGGGGGAAGCCCGGAGTAGAG







GAAGAGGCCGGACCCCCAGACCCGCCATTGACTATGTCCGCCATT







GACTATGTGTTCTGAGAGAAATTTTTTTTTTTTTTTTTGAGACTG







AGTCTCACTCTTTCGCCCAGGCCGGAGTGCAGTGGCGCGATCTCG







GCTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GNAI2. In some embodiments, the sequences from gene GNAI2 comprise or consist of:











(SEQ ID NO: 90)



TAAGCCGATGGCGGCTATCGCGGAGGAGACCCTGGCGAGGTGGGG







CCCCGCGCGGGGCAAGGGGGATGGGGTGCCACAGAGGGCTAGTTG







CAAGAGGACGCGTGACGGCTGCAGCTCTGCGGAGACCGGGCCTCG







ACGGCCAAGGAGTGGCAGGTCGGCGGAGGCGGGGGCCAGGCCCGG







AGGCAGAGCGAGACAGGAGAGCAGGCAAGGCGGGGGCCACCGTGA







GTGGGGGCCGCGAGGCGGGGGCTGGGGCGCGGGGAGGTGCTAGGT







CGGCCTCGGCTCCCGCGCCGCACCCCAGGCCCGCCAGGAGGCGCC







GCTGCCCCGCGAAGCCGTTCCGGCCTCTCCCGGCCACCGAAATCC







CGCGCTCCGGCCTAGAGAGGCCCCGGAAGAGGCGGTGCAGCGCAC







CCCGAGGCCGCTTCCGGCTTCGGCTCTGCAGGAGAGGCCAGAATC







GGGGGAGGACGCCTTGGGCGGGGCGTGGGTGAGAGAACCTGGGGG







AGGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TKT. In some embodiments, the sequences from gene TKT comprise or consist of:











(SEQ ID NO: 91)



GGCAATTGCCCCCCACTTAGCTACCCAGCCAGGTCTAGCAGTGCC







AGAGGCTGTGCCTGTGTTTGCCTCAACACCAATTCCAGAGGGACC







AGGCATGGGGTGATGTGGTTTGGTCCCAGGGGGGTGCCCTGGGCT







GATGCCTGTTCACCAGTGTGTTGAGGGCAGACCTGGGATTGGAAC







CTGGGTCTCTGTGAGTCTAGAGATCCCACCTTCTGTACCCTACTG







CCCCTAGGGCCCTCAAAAGAGGAATCATGCAGTAGGCTCCCAGGA







GGGCCCTGGTCCCATAGGATGATGGGAACAGCCTGGGGCTCAGGT







CCTGTGCGTCTCCAGAGCCCACAGGCCCCTGCAGAGAATCTAGAG







GCAAATACTGGGACAAGGACACCTGAGCCATAGGCACAGGCCCAG







CCAGCAGCCTGGTGAGCCCATCTACACTGGCTAGCCATGCCCCAA







GGACCTGGGAGGCCTGGCTAACCCAAAGTAGAGGGACAGGAGGGG







CAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TKT. In some embodiments, the sequences from gene TKT comprise or consist of:











(SEQ ID NO: 92)



TGTCTGTCTCTTTAAAATAAAAAAAATCAATGAGTTATGTGATGG







GCTCATGGCTACAGGTGGAGAAAGGCAGTGCATATGCAGCCTCCT







CCATCCTTGACTAAGGCTGACAGAGGGCTGGGCCCACCACTGCTC







ACCCTGAGGCCTCGTCTTCTGACTCCCCTCCTTTCATTTCTAGGT







GGCATTGGTGAGGCTGTGTCCAGTGCAGTAGTGGGCGAGCCTGGC







ATCACTGTCACCCACCTGGCAGTTAACCGGGTACCAAGAAGTGGG







AAGCCGGCTGAGCTGCTGAAGATGTTTGGTATCGACAGGGATGCC







ATTGCACAAGCTGTGAGGGGCCTCATCACCAAGGCCTAGGGCGGG







TATGAAGTGTGGGGCGGGGGTCTATACATTCCTGAGATTCTGGGA







AAGGTGCTCAAAGATGTACTGAGAGGAGGGGTAAATATATGTTTT







GAGAAAAATGAATTGGCCCTGAAATGTTTTCTTTTTTGATGTGTT







TGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CLDND1. In some embodiments, the sequences from gene CLDND1 comprise or consist of:











(SEQ ID NO: 93)



GTATGTGAGCATTCAATAAGTACTTTTATGAGAATGAGTTTTGTT







TTAAGCTTAATTAAAAACATGCTTGCTTTCTAAAAAAAATTCTTA







ATGGTTATATGTACGAAGGGATGGGGAATTGGGTAAAGGGTGCAT







TTGGAATAATTCAAAATAATCTTTCTTATTTGTCCGTAAACTCTA







ACTTATCTTCTTTCTTCAACTTCTAAACAGAGCAGTCTGAATGCC







AGAATGGATAACCGTTTTGCTACAGCATTTGTAATTGCTTGTGTG







CTTAGCCTCATTTCCACCATCTACATGGCAGCCTCCATTGGCACA







GACTTCTGGTATGAATATCGAAGTCCAGTTCAAGAAAATTCCAGT







GATTTGAATAAAAGCATCTGGGATGAATTCATTAGTGATGAGGCA







GATGAAAAGACTTATAATGATGCACTTTTTCGATACAATGGCACA







GTGGGATTGTGGAGACGGTGTATCACCATACCCAAAAACATGCAT







TGGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CLDND1. In some embodiments, the sequences from gene CLDND1 comprise or consist of:











(SEQ ID NO: 94)



TATGTTGCACTGAGGATTTAGGGAGAGAGCAAGATTTCTGTTGAG







GAGGTTCAGAAGTATAAGGAATCGGGAGAGTAGATAGGAGTGAAG







CATTTGGCAGCGTAAAGAAGCTTGGCTTAGCTTTAATGGATTAGG







GAGAGATTGCTATCTAGCATTATTACATGTTATTGTTCTCTTTGC







TATTTTATACACCTAGAACAACCTTTCCCTCGGGGTTAACAAACA







TCTTCAACTCTGTTTTCTGTATGGCTCCTGACTCTTACCTTTGTT







TTAGGAGGCTTCCCTCTTCAGAGGAAACTGGGAGAAGCCAATTTC







CACTGCTTCTTTTTCCTGTGCCTTTTTAACACTCAAACCCAGAAC







ACTTAATCCTCTGCAGCTCGTGCAAAAATTCAGTCTGTAATTTAA







AATGTGTGCACGGTACACTGCTGACCATGGGCATGTCTATTGTTT







GTATCTGTAATGTGTATGTCCATTTCAGTGTTGTCTGTTTTAGTA







TGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CD200. In some embodiments, the sequences from gene CD200 comprise or consist of:











(SEQ ID NO: 95)



TGGAGGCAGACACCTCTGTTCCACCTGCTTCCTTTGTAGATTGTG







TATGTGTGTGTGTTTGTGTGGAATGCATAGGGCTGCTCCATCTTC







TTTACTTACAGTGGTCACTCTGCCTGTTATCCTTTGGCCTTTTCC







TGCTCTTTGCTTCTACTTCTCCAAGCATGGACAAGATTGGTGAAG







CTGCTATCCTACCTGGCAGTTTACATCTTCAATCTCATTGTACCT







ACTTCGCAGCTTACACAGATCATTCACAATTTCTTGTAACTGAGA







GTTGCTCTCTTGTTTTTCTACTCTGCTACTATTCTTTAAAGATTG







AACAAACATATTTGAACCTATATATTTCTCTGGCATCACGTAGGA







ATGTAAATAAATGTTTTCTTTTTTGCATTTTCTCTTTCTTTTCTA







TATTTGACATTGAATATACATTTTATTTATAATCAGGAAAAAGTG







TATGTGTGTTACAATCTTTAAATATAAATGTTCTTTTATGATTCC







ATAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CD200. In some embodiments, the sequences from gene CD200 comprise or consist of:











(SEQ ID NO: 96)



TGAGAATCTCTGAGAATCATTGTCTGTGTCTGGAAATACTATTTG







CAAGAATGTTTGGAATATAGCCGTAGTGCCCAGTTTTTCAGGATT







TTAACCACAGAAAGGGTCATGAGAAGATAGCCTTTCTTGTCTACT







ATAGCTGTGTTTATGATTATTTGGAGAGTTCATGGGGCTACACTG







AGTTCTTTGGCTGGAGCTATCTTTTCTCTGAGGGATCCTGCTGTC







AACAGGAGAAATTTGAGGACAATGGTGAGGGCAACAAGTTCTCCA







TAAGCAGTGATATGATTCAGACCTGGCTTTGCCACTAGCAATCAT







GCGACCCTAGAAGGGTCCTATCCACTCTCCACTGGAGTTTCATTG







TCTGGACAATAAGTGTGAAATAGATCATTTCTAGGGTCACTTACA







ACTTCAACTTTTTGGATCTAAGGACAGAGCCAGATTGGACTAGCC







TTGAGCCAGTTATGATTGTCCTTCTCATTTTGCTTGGGGAGATAG







CTCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MCM2. In some embodiments, the sequences from gene MCM2 comprise or consist of:











(SEQ ID NO: 97)



TGGGAGCCCACGGGGTCCCCAGGAGCCGATCTGAGGAAGTCATTG







TGCAGCAGAGGGTTCCCCTCTTCTGCATATCCTGCCAGAGTGGGG







AACAGTGAAAGTGGGACCTGGGACACCTGGGTTTCCTGTTGAGTC







ATGTTCCTGGAATCACCTTGGATGTTCTCAGAAGGCATGGGAGGA







GAGCCCAGTGCCCCTCTGGCCAGGATGGAGTTGGCTGTTGGTCTC







AGCAGCGAATGCGTAAAAGAACCCAGGCTCTGTCACCCACTGTGT







TCTTGGTTTTTCCTCCTGGGGAAGCAGTTATGCTTTCTGTCTAAA







TTAGGCTATTTTGGGCCCTGGTTAGGGTAAGCTCTTTGCTGTCTC







AGACAGCAGGTGCTAAGTACCCACCTATGTCTTCCTCTTCCACTG







CCCTCATCCCCTTCTGCCATCAGAGCAGCCTGGGGTCCCTGAGAT







GGGAGGATAGGGGAATCCACCCTGATGGAGGTGCTCCCCTGTGTT







TCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MCM2. In some embodiments, the sequences from gene MCM2 comprise or consist of:











(SEQ ID NO: 98)



TATAGCTCACATGTGCCCGGTTTCCATGAACTGTGGTTTGGGGAC







CTCAGGTGAGGCTTGGGGTCATACAGTGTGCCCAACACAGGGGAC







AGGTGCTGCAGGGGCCATAGGCTGTTTTCTAGTCCTGTTCCCTCG







GTCTCTTCTCATGTCCAGAAGTCGCCCTGATTTCTCTTTCCTTCA







CAGCACCCCCGACCCCCACCCTACATAACCCACAATCCACCAAAT







CCTGTTGTCCCAACTCCTGAGCCTACCCACCTCCCTCACTTCTGC







CTCTGACTTGAGGCACTACCATCATCTGAACTGGCCTCCTGGCAC







CCATGTAGACTGTCTTCCAGTTGAAGGCATAAATCAGATCAGCCC







CGTCCTTAACAACTGCTTGAGGCTTTCTGCTGTCATCAGAATAGA







ATCCAGGGTCCTCCCCTCCGGCCCGCACCCGCCATCTCTGCAGCT







ACCTCCTCCTCGCTCCCTTCCCTCACCTGCACCTGCACCTGCCCT







TCTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TMEM108. In some embodiments, the sequences from gene TMEM108 comprise or consist of:











(SEQ ID NO: 99)



AATGGGATAAACCTGGTTTTCTGAGTGGAGGCAGCTATTGATAAT







TTTACTTTTCTTATAGCTGAAGATGAAGGAGATTTGAGGTTCTCT







GAGAATTAAAATTCGGCTGGAGTTAGCCTAGGGTGAGACCCTCAG







AGCTGTCTGTTCATGCCATTGCCTCAAGCCTGTGGACGTTTTTTC







ACCCTGGGGCAAGAAGAGGCTCCAGTTTGCCCAACTGCTGTGTGT







GTTCCAGGCCAGTGTCCAGTGAGGCTGACTTGGCAGCTGCCCTTT







CCACAGCCAGCCAGCCAGCCCCTGAGTGTGTTTTAAAAGATAAGC







GACAAAAAGCCAACCCCTTTTAGAGGGGCTGGAACAGGCTAGTAG







TTGCTAGGCAAGTTAAAGTAATTCAGCGTCCTTGCCAAACGTCTG







CCAGGCAAATAGGGAACTTTCAGAGAAGGGGAGTGAGATGGTGGT







GTGTGTTGGTTGCCATAGGAATGTTTCCCTGATTGGAACAAGACC







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TMEM108. In some embodiments, the sequences from gene TMEM108 comprise or consist of:











(SEQ ID NO: 100)



TAGGTATTTTGTATTTATGTAAATATTATGTCAGATTATGTCTTT







ATTTTAGCTCTTAAGGTAGTAATTAAATATCTGTTCATGAATTAT







AGTTTTTATCGAGTTCTGATTTATAGGGGGAAAAGGCACAGTTGA







CCAAATTGTATGAAACCTTGGACCTTGAAGAGACCCTCTGATGTG







GGCTTTACTGCCGCATTAACAAGCACCCTGTGCATCTGGTGGCAG







CTTCCAGAATGACAGCTGAGGGTCTGAGTGGAAATAAGTGGCTTT







TAGCAAATGTTGAACCTCGAATGTGCTATTTAACCTCCCTGATTA







ATTGATTATCCAGCTCCGAAAATAAGAGGTGATATAGCTATCCCC







TTATCTCAGCATGGATGCTGTTCTCATTTGAATACAATTTGGGGG







GTAGGTGGGGGTAGTATCAGATCCTAGGGTACATGCTATGATAAT







GACAGGCTTAATTATCAGTCAGTCAATATTGTTTAGAACAAATGT







TTTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene EIF4G1. In some embodiments, the sequences from gene EIF4G1 comprise or consist of:











(SEQ ID NO: 101)



GCCAGGTGAGGGGCTGTGGGGAGGGGAGTAGGGGACCTGGGTGGG







AGCAGTGGAAAGCTTTGGCCAGTTTAGGTCTAGGATGGAAACTGG







AGGCCCCATACCTGGCCTTAATCCTCTGTGTTCTTTCATGCGGCT







CTGCGCCTTGCCCTGTTGATTACTCACGGTGGCTGGTCCCTTGGA







TAGTATGTTGTCAATGAGGGTGGCATTACTGGGATCCTAGTGCCT







CTGAGAATTGGGGGCAGTGCTTTGGGCCCTGCTGCCAGCTCTTGC







TTTCCTATTTGCTCCTTTTACCGGAGGCTGCTATTGCTCTATTGC







TCTCTCTGCTTTCTCTTTGGACTGTGGTGAGGTAAACACTCCAGC







TGGTCCTTGCCTTTCTCTGTTCCCCAGCTTCTTCCTTACTAAGTG







GGAGGATTCTTGTCCTGTCTTATTGCCTTCATTCCCTCCCCCCGC







CATCACCTTGGGGGTAATTCTCTTCTTTCCTATCCCCATGTGCTC







TCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene EIF4G1. In some embodiments, the sequences from gene EIF4G1 comprise or consist of:











(SEQ ID NO: 102)



TAATATCCCTTTCCCAACCCTGGACAGTCTTCTGGTCTCATCCTT







ACCCTCCACCCTAGTCAGGGGCTAGACACAGGGAATGGGGATCTT







CAGGGTAGTGACTTGTTTGGTGTCCAGGTTCTCAGTTGGAGGCCG







AGTGATGCATGTTAGGGGCTACATCTTGAAGAACGCGCATCTGGG







GACTGGGATGCTGTTGCATGTTATGGTTAGCTCCTCATTACATGC







TGGTGGAGCAGTGCATGCTGGGGATATGTTGCCCGAAAGGGCCTT







ACTTAGGCATGATGCATCTCGGTGTTAGGAAGCCTCTGCAGAGGG







GTAGTTTGGTAGAGGTGACGTGTGTATGCATGGCAGTGAGAGAGT







AAATCTCTGCAGGATGCAAAGTGTACATCTGAATTTGGAGGTTTG







GAGGGCATGTGTGTGTACAAGAAGCCGCTTAGCCATTTGGTCAAG







TGGTCTTGGAAGGATTGGTCGCCATGTTCTGAAGCCCACAGTACT







TCTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TACC3. In some embodiments, the sequences from gene TACC3 comprise or consist of:











(SEQ ID NO: 103)



CTATGTCTAGCAGCAGCCTCTGTAGGTGTCTGGCAGGACGGGAAC







ACCAGGCCTCATCTGGCCTCCCAAAAGCTTGGGCTCCTGGAGGTG







CTACTGTGGGGATCTGTCTTAGGCTGAGTGTCAGCCGGCGGCAGG







TGACCGCCTGGCCCTTGCAGTCCCTCCAGGACCACCACGAAGCAC







GACGCACATCCTGGAGCTCGCCATACCGGCTCCCAGGCCTCACCC







TAGGCTCTAGGGTGTTGGGCGCCGCCTCTTCAGGGACCTCTGGCC







CAGGCTCCAGTTCCCTGCGGATCTGATGTGGAGGAAACAGCTGTT







TCCTGGGCTTTGCATCCGGCCTAGAAGATCCTGGGTGGAGGGACC







CATCACCCTCCGAGGCTCACACCCACTGCCCACCTCTCCTCCTGG







CGCTCGAGTCCCTTGCCTCATTCCTTGGGCTGTCTCTGGCACCCA







TCGTTTCGGTTGCCTCCTCATCCTGAACGTTTGCTTTTCTTTTCT







CAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TACC3. In some embodiments, the sequences from gene TACC3 comprise or consist of:











(SEQ ID NO: 104)



TATGTCTCCGCCACCCTGGTGTCCTCACCTCGGAGGCTGATGGAC







TCATGGTCCAAGCCAGCGGGGCTACAAGCTGCTGTCTTTGCACCT







TGGTCCTTGGCCAAGCCTCCCCTTGCCACGGGGGCATGGGATGAC







CTGTGAACGAGACAGGGTGCAGGAGTCACAGCATAGCTGGTTTGC







GAGGCAGCTGAGGGCGGCAGGAGGCAGAGTGAGCAGGGAGGCCAT







CTGAGCCGTCCGCTGTTGTGTGACAGACGACTCGGCTATGATCAG







GCATCTGTGAGATTTGAGACAGCGCTGGTGCACCGGGGCCTGGGG







CACCTCGGAAAGGTGTCTGGGCTGTCCGAGCGCTTGGGTCCCAAG







GGTTGGGGACCCAGGGCATGCAGTATCCAAGGCGGGAGGCCCACT







GAAAGGTGGTCACGGCTGTGCAAGGCCACCCTGGTGTCCTCCTGG







CCACGGGACTCTGAGCGCCCCCCACCTTCTGTGCTGGGCAAGGCC







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ADD1. In some embodiments, the sequences from gene ADD1 comprise or consist of:











(SEQ ID NO: 105)



GCCGGCCGGGGTGTACTCCGCGCGGGACCCTGGGCCTCCGCAGGC







CCCGGCGGGGTCGCCCCGAGGCGGGCCTGGCGAGTCCAATCGCCC







TGCCCGCAGGGGACCCGCCCCTCATGGGTCCCGCCAATCGCAGGG







TACGTTGCTCGGCAGTGCTGCGCGGCTTGGCCTATGGGACGGCGC







GTTGTTGGGCTGCAGCGCCGCAGGCCGCACCCAGGTCGGGCGGTG







GGGGCGAGCGGAGGGGCTGAGGGGCGGAGAGGCCTGGCGGGCCGC







TGCTGCGGGCCAGGGGACGGGGGCGGAGCCGGAGCCGGAGCCGAC







GGGCGGTGGCCGCACTGGGACCCCGGAATCCCGCGCGCTGCCCAC







GATTCGCTTCTGAGGTGAGGCTAGCTAGCGGGGGCGGCGGGGGCC







CGGTTCGGGCTGAGGCGGCGGCGGGGGCGCGGGGAACTGGGCCGC







GGTTGCGGCGGGCGGGGGCCGCGGGGCCTGCGGCGGCCCGGGCAG







CCTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ADD1. In some embodiments, the sequences from gene ADD1 comprise or consist of:











(SEQ ID NO: 106)



TAAGTTAAAGAAAGATTTTTTGTGTGAAAAATTGACTGAGATCTT







AATGGGGATAGGAAATAGAATGCTGATAGGTCGACTGTGTCTATG







AGTAGAGGCGCCTAGTGAAAACAAGCTTGGAGTGGATGAGTAGGA







GTTCAACAGGCACCAGAGAGAGGAGGGCATTTCAGGCAGAGGGTG







TGAATTGTATGAAAGTGAGTTTGTAAACTTTGGAGCCTTGAATGG







ACAAAGGCCCGCGATGATACCTTTTTTTTTTGAGACGGAGTCTCG







CTCTGTCGCCAGGCTGGAGTACAGTGGCGCGATCTCCAGTCACTG







CAATCTCCGCCTCCCAGGTTCAAGCAATTCTCCTACCTCAGCCTC







CCGAGTAGCTGGGACTACAGGCGCGCGCCACCACGCCCAGCTAAT







TTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGA







TGGTCTCAGTCTCTTGACCTCGTGATCCGTCCGCCTAGGCCTCCA







AAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene UCHL1. In some embodiments, the sequences from gene UCHL1 comprise or consist of:











(SEQ ID NO: 107)



CCTCCCGAGTAGCTGGGATTACAAGTACACACCACCACACCTACT







AATTTTTATATTTTTTGTAGAGATGGGGTTTCACCATGTTGTTCA







GGCTGGTCTTGAACTCTTGTGAGCTCAAGTGATCTGCCTGCCTCA







GCCTCCCAAAGTGCTAGTATTGCAGGTGTGAGCCACCGTGCCTAG







CCAGATCTCAGTGTCTTTATAACTACAACTGGCTGCCACTTTCTA







GCATTGACCTTGGTTGAGATAGTTAGCCTCTCTAAGCCTCAATTT







TCTCCTCAGTAAAAATCCTGTAGTAATAGCATCCTCATAGAGTTG







TTCTGACAGTTAACAGTATATTTACCTTAGTGGGCTTAGAATAGG







GCTTAATGTAAGACATACATTAAATATTAGCTATAATTTCTAAAA







ATCAAGTCAGAAAAATTTCTTGACTTTCTTTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene UCHL1. In some embodiments, the sequences from gene UCHL1 comprise or consist of:











(SEQ ID NO: 108)



TATGTTTTACTCCATTTTTGGAACCCAGTGTAGTTTCATGTGTTC







TTTCAGACTGAATTTCTCTTGATATATTGTGTGACTTTATGGCAC







TTGGCATATCATTGTTTATAAAGCCACAATAACAAAGTATTCTCA







TGAGGGCACTTAACCCCTTATCATCCCCAGAGCAACTTCCTCAGG







CAACTGACTAGCATCCCCTAGTATCATCCTTTCTGGGCAAAGAGT







ACGTCTGAAAAAGGATCAGTGCCTGAATATGACCTTACTTGTCTA







AAAAGAATCTGAACATGTTGGAATAACTTCTATTAACTAGTTTAT







GGCATCAAGAAAGACGACCCACCTTGTAAGATTCATTCCTTTTGT







ATTAATGGAGAAAGACTTGTAACTCTTGTCTCAAGCCAAGATACG







ACCTACAGGCTTACAAAGGCAAAGGAATAGTGAAGGCAATCTACA







GTCATTGGAGGGCAGATAAAATCTCAGGCGTCTCCTCCTCTCGCC







TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene OCIAD1. In some embodiments, the sequences from gene OCIAD1 comprise or consist of:











(SEQ ID NO: 109)



ATTCCTAGGCTGCTGGCTCTCAGACCCGCAGTGAGGTCTGACGTC







ATTTCCTGCCGTAAGTATACAGTGCCTCCGGGTCGCGGTCATTTT







GAGCCCCTGTCTGGATGACTTCTTGCGGCTGTTCTACCCCTCCCC







CTCCCCGCGGTACCTTGCACTTTTCTCCCTCCCTGCCCCCTCTCG







AGTCCACCCTCCGGGCCTTCTGCCCCTGATCGCTTGGTTTTCCTT







GCAGTCGCCTGCTGCTGTCGTCGGGAGGTGGGTGAGGTGACGCAA







ACAGCCCCGTTGTTGCCCTCCGCGTATCCCCTCACCACCTTTGCG







GCCATCCACGACTTTCGCACCTTCCGCCATTTTCCTGCCTGTGAG







GGTGGACAGATCGCGCTCGGGTCTCGGCCTCCTGAGTGCCGGTGA







CTGCGGGAGGCGACGGAGTGCTTCTGGGGGTGTGAGCTGGGGAAG







TTCGTGGTCACGGATGCGTGTGGGGTTGCTGCTCAGTCTGTAACG







GCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene OCIAD1. In some embodiments, the sequences from gene OCIAD1 comprise or consist of:











(SEQ ID NO: 110)



TAATCAGCGGTTGTAGGCCGCTTAGGCTTTAATCCAGCGTTGTTT







TGTGTAAAGAACTTAAAATGGTATTGTCTTAAGCGCCGTGTCAGC







CCTGACAGTCTTCCTGGTGTTTCTAGGAAGGATGCCGTTATGGGA







AATAAGAGGGTTTCATTTTTTCTCATACGGTTGTAGGGTAAAGGT







AGTTTATTCAGCAGGCTCCATCCATCCATGAATTCAGCTGACCTT







TTTTATTTCTTTTCTCTAATTAAGGGGTTGGCATGTAGTAGGCCT







TCATGGCTTATTGACTGAATGAATTATGGAAGGGCTCAGTTGCTG







TCCTCATTGTGTATGCCATTTCCTGTTTCCATGCAAATTGTTTTT







AAAATGAGAATAATCATGATCCCACGTAAACGTTCTGTAAAATAC







GGTTACTTGATCTGTTGGTCTGAGTCCCAATATATGAGTTTTTAA







AAAAAGAACCAAATAAAAAATGTATATTCTTGGAATTTTATGCAT







TTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SPP1. In some embodiments, the sequences from gene SPP1 comprise or consist of:











(SEQ ID NO: 111)



ACGTATTTGCTGCGATATTACTTATCTTCTCAGTCAAATCCATTG







GTTTACAAGTATTGATTGACTGCCTGCTATGAATCTAGGCCAGTA







CCAAGCACAGTATAGTTTTTAATAAATATAAGTTTATAAAACCAA







CCCAGATATTTTAAATATAATAATATCTAGGCATGTATGATGAGT







TATCGCATGTAAGATAAGTTATATGAAGTTGTGTGACTTTTTTTC







CATTAGTCCACATACTGATCTAAAAGCAGAAAATTCCAGCTTTTG







CTTTGTTTAGTGGATTGCTAAGTTTAAAATTCACATTGGATATTA







GTCAGAACTGTTTGTATGACCATAATATTCACAATATTGTCTGAG







ATATTAGCTGAGAAGCCCATTGTGAAAAGAAAGTCTATGTGTGCT







GTTTGTATCTATTGTGATTGTCAGCTGATGTTAGATCACATTTTC







TAACCAAACATAAGACCAACCAAACTCTTTATTATAATTATTTGA







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SPP1. In some embodiments, the sequences from gene SPP1 comprise or consist of:











(SEQ ID NO: 112)



TATTCTGTGTTCGTTTGTGTGTGGAGGGGTGTGTGTGTCTGTTTT







TCAACTGATTTGAAAATACAGGTGTTGAATCCTAATAATAAACCA







GAAAAATTAACATCTCCAGAGAAGATAGAGGTCATACTATTTGAG







GCAAGAATTAGCGTCTTTTTAATAAACGAAAATATGGCAAAGATG







CATTTTAGAAGGCACGTGGAGCTATAACAATTTAAGAAATACGTG







AAGAGCTCAAGGCTCAGCCTTCTAGAATCCCAGAAACTTAAAGCT







AGTAAAAAATTGGGGAAGTCTCTAAGGATATATGCCTGAAAATAC







ACACTGGTTATCTGTGAGTGTTAGGATTACTGGGTGGTTTTTAGT







CTATTCATTTTGCTTACCTTTATTTTCTTCATATTAGTTTTTAAA







AATTATAAATGTAACTTATACATCCATTCTCTCTGAGCCTGTATT







ACATGTGTCATGAGAATAGATAGATAGATATGAAAAAGTGAAGAG







AAAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NREP. In some embodiments, the sequences from gene NREP comprise or consist of:











(SEQ ID NO: 113)



GCAGGGAGATGATCTCCTCCTCCTTTTTCCAAGGCTGCACTTCTT







GGAAGTGAAGCCGGTTTGGGTTTTGTTTCCCTTCCCCCTCCTCCC







TCCTCTTCCTCCACCCCTTTCCCCTCCCCCTCCCTCCCCCAATCC







CCCCTCCCCCCCCAATCTGTCTTTCTAGCATGTTGCCCTTTTTCA







ACCACATTTGTGTTTCAGGTGTAGAGAGGAGAGAGAGTGAACAGG







GAGCGGGGCTTTTGTCTGTAAGTATATGCCATTCCTATCCCCGGC







TGGGAGCGCTGGTTTCTCTTTTGTGTCGATTCCTTGCCGTTGCTC







TTAGCTGGACCCTTCAACCCTGCAGAGTTCATGCTTCTTCCCTCT







AAATTTTAAAGAGCTGAAGCGTGTACCTTGGAGAAATTCTGGCAT







TTTGGTTGTGCTGCATTCAGGCTGGAATTTAACTTGGGTTTTTTC







TAAAATGCACAAGTTCGGAGGGAGAAATGAATTCTTTTGGGAAGG







GGTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NREP. In some embodiments, the sequences from gene NREP comprise or consist of:











(SEQ ID NO: 114)



ACGGCCCTGGCGGGCCGCCGGGCTGTGCAGCACCCTGGCCGCCTT







CACGCTTGGCGCGCCCCCAAACCGCCTCCTCCACCTCCTTATTTG







TCTGAAAAGCAACCGCACGTCGAGGCGGAGAGGCTGGCCGGGGGC







GGGGAGGAAGGCCGGGGATTTGCGCGCGCCGTGCTCCCGGGGCGG







GGCCGGGGCGCAGAGCTGCGGGTCCGCTTTGTCTCCCCGGGGCGC







CGCGCGGTGCAGTGGGGACGGCGCGGCGAGCGGGGCGCCCGGGGC







GCGGGTGCGGGGTGTCGGGGCGCGCGCGCCTGTTGGCCGCTCGCC







GACGGGGCCCAGAGCCGCCGATTCCTGCCGAGTAAGCAGTCTGGC







TCCTAGGCGGCTGCACAGTGGCAGCAAGGCTGGGGCGCTGGCTCC







GCGCCAGCTCCATCAGAATTAGCTCATTGTTCACTAGCACCCTAG







CGGGAGAGAGAGGGAAAGGGAGAGAGAGAGACTCTTAGAGAAAGA







GAGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene HSD17B4. In some embodiments, the sequences from gene HSD17B4 comprise or consist of:











(SEQ ID NO: 115)



CAAATCTTTACCATCCCTAAGGGGGACTTTCCTTCCCAGGGGCTA







TCTCTGGAAGCCCCTCAAGGATAGGGGCCGCATGCTGTTTCTCTA







GGTCAGCAACTAAACCCAGAAAACGTTTATTGAGTGAATGATGAA







ACGACAGGTGAATAGATGAACGCAAGGTGTCGAGTTAACTATTCT







TCTACACAAGTCCTAGCAGCTCCCATTGCTTCCAGCCGCAGAAAT







GGCCCCTGGAAGGCAAGTCTTCCAGCGAGTGGAGTCACTCTTAAC







TACATTTCCCAGGATTCCAAGGGAGCCGCGCGCTCTGCGCTCATC







TTCCTACCAGAAATCGGCAAGTCACTGACCCTCGTCCCGCCCCCG







CCATTCCCCGCCTCCTCCTGTCCCGCAGTCGGCGTCCAGCGGCTC







TGCTTGTTCGTGTGTGTGTCGTTGCAGGCCTTATTCATGGGCTCA







CCGCTGAGGTTCGACGGGCGGGTGGTACTGGTCACCGGCGCGGGG







GCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene HSD17B4. In some embodiments, the sequences from gene HSD17B4 comprise or consist of:









(SEQ ID NO: 116)


TACAGCCCGCTTCTCCCCAGCACCCCGGTGTGGGCTTCCCAAGGTCCTG





CCTGAGAGGAGAGGCCAGGCTGGGCTGCTGATTGCAAAACTGGGTGAAA





GTTCTCCCTGACCCTTATCTGTGGGCATCGATTGTTACTCTTCCTGCAA





TTAACTCTCTTAGATCTTTGCCTAGTCTTTTAAAGGACTGAAAAGCCGC





GAGGGGCGGGGGCTGGAATTCGCCCCCTGAAGCGCAGAGATGTCAGCTC





CTGAAAAGTCATTCGGTCGTTCAGTGTTTGTTTCCCTCTGTCGTAAGAT





TTTAAGTTCGTGAGAGGACCTTCTTTAAAGAGGGCGTCTGATAAGAGCC





CTTCCCCGTTGGAGTTTGTATGCTTAGCAAGTCACAATCTGTTCTCGAA





ATCCACTGGAGTCTTGGCAGAGGTTGTAAGCTCAAATGCGCACAGGGGT





CAGGCGTATGATGGAGAAAGAAAATGGGAGTAGGATGGGCACATCTGAG





GAACTGGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene LMNB1. In some embodiments, the sequences from gene LMNB1 comprise or consist of:









(SEQ ID NO: 117)


TGGCCGGGGACACTCTGAGTTTCGCGTGTGGCTTTTAGGGACGTTTATA





TTTGAATTTCCCTGAACCGCCGAGTGTGGGCGGTGGCGCAGATCCGTCC





CGGAAACCTCCGGGCTCCTTCCCGCCTTTCTCAGGCCCGGCCCCTCCAA





GGGGTCCCCGCGGGGCGGCGGGAGGGCCCTGGGCCCAGAGCCGCGCGGG





TGGGCAGTCCCAGGCGTCCTTCCTTACAGCCCTGAGCCTGGTCCGGGAA





CCGCCCAGCCGGGAGGGCCGAGCTGACGGTTGCCCAAGGGCCAGATTTT





AAATTTACAGGCCCGGCCCCCGAACCGCCGAAGCGCGCTGCCTGCTCCC





CATTGGCCCATGGTAGTCACGTGGAGGCGCCGGGGCGTGCCGGCCATGT





TGGGGAGTGCGGCGCCGCGGCCCGCGCCACCTCCGCCCCCCGCGGCTTG





CCTCCAGCCCGCCCCTCCCGGCCCTCCTCCCCCCGCCCGCCGCTCCGTG





CAGCCTGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene LMNB1. In some embodiments, the sequences from gene LMNB1 comprise or consist of:









(SEQ ID NO: 118)


TGAGTGGGTGTGTGTGTTTTCTTACAAAGGGTATTTCGCGATCGATCGA





TTGATTCGTAGTTCCCCCCCGCGCGCCTTTGCCCTTTGTGCTGTAATCG





AGCTCCCGCCATCCCAGGTGCTTCTCCGTTCCTCTAAACGCCAGCGTCT





GGACGTGAGCGCAGGTCGCCGGTTTGTGCCTTCGGTCCCCGCTTCGCCC





CCTGCCGTCCCCTCCTTATCACGGTCCCGCTCGCGGCCTCGCCGCCCCG





CTGTCTCCGCCGCCCGCCATGGCGACTGCGACCCCCGTGCCGCCGCGGA





TGGGCAGCCGCGCTGGCGGCCCCACCACGCCGCTGAGCCCCACGCGCCT





GTCGCGGCTCCAGGAGAAGGAGGAGCTGCGCGAGCTCAATGACCGGCTG





GCGGTGTACATCGACAAGGTGCGCAGCCTGGAGACGGAGAACAGCGCGC





TGCAGCTGCAGGTGACGGAGCGCGAGGAGGTGCGCGGCCGTGAGCTCAC





CGGCCTCAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SKP1. In some embodiments, the sequences from gene SKP1 comprise or consist of:









(SEQ ID NO: 119)


GTACGCAAAGAGAACCAGTGGTGTGAAGAGAAGTGAAATGTTGTGCCTG





ACACTGTAACACTGTAAGGATTGTTCCAAATACTAGTTGCACTGCTCTG





TTTATAATTGTTAATATTAGACAAACAGTAGACAAATGCAGCAGCAAGT





CAATTGTATTAGCAGAATATTGTCCTCATTGCATGTGTAGTTTGAGCAC





AGATCCCAAACCTTACGGCCAAGTTTCTTCTAGTATGATGGAAAGTTTC





TTTTTTCTTTGCTCTGAATAAAACTGAACTGTGGGTTCTCTATAAGTGG





CATTTTGGGCTTTCCCTCTTTTTTGTAAAGCAATGTCTGCCTAGTTTAT





TGTCCAGTTAACTTTAGTGACCTTTTAAAAGTTGGCATTGTAAATAAAA





CAACTTGCAAAAAAGTTTTCTGGAATAGAATTAACAAAATATTATCTTT





ATTCATGAGTTGGAAACTGGAAAAAGGCTTCTTGAAGTAAATGTTCTGA





GTGGAGCTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SKP1. In some embodiments, the sequences from gene SKP1 comprise or consist of:









(SEQ ID NO: 120)


TATAGTGCTATAACTGCTTACGTTTGAACTCTTTAAGTGTGGACAAATA





TTTTTGCCAAGACCTGATGTGGTATTTGTGTGCATGTGTACACACATAC





ACAACAGTAGTGGCCGTTATTTCACATTTGAGCTAGTATAGTTTGGTTA





AGGAAGCAGAAGGATTCAACATTATAGAGGTATAACAAAGAAGGTCACA





TAAAAGTCTTTCACAGAAGAACTTAGGAGTGATTTATTCTTCTCCCTTT





AATTTCTTAAAATTAGCATTTTAAGCTTTATTAGATACGGAGTGTCAAT





CATTAACTTTGGATTTAGTTTAAAACATCAAGTATACTTTAAGGAAAAC





AACTGTCTCTTTACAGGCTGCAAACTACTTAGACATCAAAGGTTTGCTT





GATGTTACATGCAAGACTGTTGCCAATATGATCAAGGGGAAAACTCCTG





AGGAGATTCGCAAGACCTTCAATATCAAAAATGACTTTACTGAAGAGGA





GGAAGCCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene REEP2. In some embodiments, the sequences from gene REEP2 comprise or consist of:









(SEQ ID NO: 121)


CCTTTCAGATTAGAAAGGAGCAGTCCCATGAGCCCCATGGGGACCCCAG





TGCACTAAGAGTGAGGGGCTGTGCAGGGTCACACATGGGGCCGGTGCTG





CTGGACACAGGGCACCTCCCAACTCCCTGCCCTGTACTCTGCGCTTGCC





CCTGTCCCAACAGGTTCCCCTTCTACTTTGAACTGAAGATCGCCTTCGT





GATATGGCTGCTGTCCCCTTACACCAAGGGCTCCAGCGTGCTCTACCGC





AAGTTCGTGCACCCAACGCTGTCCAACAAGGAGAAGGTTTGCCCCCACT





CTCAGCTCACCTCCCAGCCTGCCCCCAGCCAAGGCAGTCCAGAGCCTGC





AGCCTCATACAGACTGGCCCTCCCTGTGGGGCCCAGAGCCCAAAGTGAC





TTGGCAGGGCTGCCGTGGCCTCCCGGAGCAGGCAGGGGCCTCTGTCCAG





GGGTGAACAAGGGTAGGGCAGGACCTCTCCTCTTCTCCCTTCCCCTCCA





ATCCCATAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene REEP2. In some embodiments, the sequences from gene REEP2 comprise or consist of:









(SEQ ID NO: 122)


TGAGATGGGGGCAGGCTCAGACTCCCAGGGCCCCTACCTTGTACAAAGG





GCCCTGGCCAGGCCCCTTTGCATCCACCCTGTACTCGGGGCTGTAGAGA





CTCTGGGCTCATGCAGCTGGAGGCTCCAGTCCAGGCTCTACTGCTATCT





GGCCGAGTCACTTTGATTGTCCGGCCATTTCCCCATCTGTCCCCAGAGG





CAAAGTGTGGCCCTGCCCCAGCCCTCAGCTCTGTCTGTGTGGGACTGGG





TGAGGACAGTGTGGGGAGGGCACCGAGCCTGGGGCTGCTGCCAGCACCA





TGGTGACCTCTATCTCCACCCCGCCCCTTCCCCCCGGCTCTCCCGGTGC





GTGGTGGTGACCCTAGGGCCAGGGGGTGCTGTCAGAGAAGCTCCGCAGC





TTCAGCATGCAGGACCTGACCCTGATCCGGGACGAGGACGCACTGCCCC





TGCAGAGGCCTGACGGCCGCCTCCGACCCAGCCCTGGCAGCCTCCTGGA





CACCATCGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MATR3. In some embodiments, the sequences from gene MATR3 comprise or consist of:









(SEQ ID NO: 123)


CCCTTTGTGGCCCCAATATCTGGCGCTTTCCACCATTCCTTGGCAAACA





GAACAACCAGAGAGCATAAAAGTCGAGTCTAAGCCAGGGACTCTCGCGA





ATCCCTTCTAAGGCATACCCTATTTAAACTTTGTATTATGCTATTCAGG





AGGTTGACAAACAGCCTTTTCGGGAATGGGACTCGAACTCGTAAGCCAC





GTCTGTTCCTCCATTTCCCGCCTCAGCTGGATGACGGAGAGGGATCTCC





GGGATTAAAGAACAACGACCGCAGGCGAGTGCACGAGCGAGGTGCGCGC





GCTGAGTAGGGGGAGGGGCCTGGCGCGCGCCGAACCAGATCGAGGGGGT





GGGGGCGAGCTCTCTCGCGAGAGTGGGTTTGTTCTTGGGCTGCAGCCGC





TGCCGCCGCTTCTCGCCAGCGCCGTTGCTGCGGGGGATTGTGGGAGTCT





CCGCGTCCCGCTCGCTGGGAGAGAGGTACCTCTCCTTTTCCCTCTCCCT





TTCCCTAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MATR3. In some embodiments, the sequences from gene MATR3 comprise or consist of:









(SEQ ID NO: 124)


TGAGCGGTCCGGGAGGGAAACACGCGGCCGGCCAAGGGCCCAGGGTGCG





GCGGGAGATTTTGGGTGAAGAGGTGCGCTGACCGGCCGAGCTTCCTGCG





CGTCCTGGGCTCGTTGTGGGCGGGGGCGGGACGACTAGCCCGTTACACG





CGGGCCGCGGCGCTGAGGGGGAGGGAGGAGCTCGCTCGAGGCCTGAGGG





ACAGACTGTACACTGGGGGCTTGCCGTTTGAGGAGGATGGTTAAGATAT





CTAAGTCCTTGGGTAGCGCCAGTGCCAAATAACCCGTTCGCCTTTTTTT





CTCTGAAAATGTTCCCCCAAAATGGAGGATTTCGCAGACTCTGAAGAGC





CTGCTTGTTTGAGCAGCGTTTCTTTCTTTTCCCGGTGTCTCGGCCTTCA





CGCCGCTCCTTGCCCATCGCCTCAGAGCGCCCCCTAAGACTCCCGGCCC





ACACGCGCGCGCGCGCGCGGCGTTAGCTGGACATCAGAAACAATTCCTC





CCCGCCCCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CAMK2A. In some embodiments, the sequences from gene CAMK2A comprise or consist of:









(SEQ ID NO: 125)


GTAACAGGTCCTCACCCTCCACAGTCCTGGCCATGGGACTAGCTCTGCC





TGGGTCTCCACAGAGGCCAGGAGACTCGCTCACTAATTCTTTCCTTCAT





TGATTCAATAAGCATTTATTGAACACCTACTGCATACTGGACAGCAGGT





TGGGCTTGGGGAGTGAGCGGTGAATGACTGAGCCCTTGCTCTAGTGGAG





AGGACAGACAAGTTCCTGGGGCGTTATGGGGCAGTGGTGAGTGCCATGC





CTGGGAAAGCCTCAAGATGGTGGCAGAATGCAGCAGGACAATGGTATTC





TGGGTCTGGAGCCAGGGGCGTTTAAGCTGAGACCTTGGAGGATAAGCAG





GTATTAGCCAAGTTAGAGTCAGAATGGGAAAGTGTGGCCGGGCGCAGTG





ACTCATGCCTGTAATCCCAACACTTTGGGAGGCTGAGGTGGGCAGATCA





CCTGAGGTCAGAAGTTCAAGACCAGCCTGGCCAATGAGGTGAAACCCTG





TCTCTACCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CAMK2A. In some embodiments, the sequences from gene CAMK2A comprise or consist of:









(SEQ ID NO: 126)


TCCCATCGCCGGAATGGGACACTGTCACCCCGGAAGCCAAGGATCTGAT





CAATAAGATGCTGACCATTAACCCATCCAAACGCATCACAGCTGCCGAA





GCCCTTAAGCACCCCTGGATCTCGGTGAGCCTTCCTCAGCAGGACTTCT





CCCAGGAGCCCCTCCAGACCTCATGATCCAGGCCCCCACAGAGTTCTGG





CCCTTCCCCTGGCCCATGGGGCCAAGAGGGCTTAATCCAAGAGACTGCC





CCTGCTGTGGTTCTCAGGGGTAATGTGGAAACGAGGAGTGAGTGTTCTG





GGTTGGCATACAACTGATGGTGCTACTGGCATTGAATGTCTGGTGCCAG





GATGCTAAACACCCTGAAGCATCTCCTAGAGCAATGAATCGTTCTTCCC





AAGATGTCAACCACACCTCCATTAAGATGCCCTGCTGAAGGGTACCCTT





GGGTGGGCCTGTCTTCCAGCCTGCCTTCCACTCACAGCCCTCTCCCCTA





CTTCCTCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CYFIP2. In some embodiments, the sequences from gene CYFIP2 comprise or consist of:









(SEQ ID NO: 127)


AAACCAGTTTTCAGAGAATTAGAGTTGGATATCAACTGGACAGCACCCA





CAACAGATTAGACTTGGATTCTTCCTTTGAGGACTTGAGAGGCCGTCAT





GAGAAGAGTGCTTCTCGGCTCCTAGGGATGGTGAGACAGGCCCTCCAAC





AGGGCTGCAGATGGGGGACTGTCTGAGGCACTGACGGCCACTGTGGCTG





CTTTCCCACAAAGGAAGCCCTGTGGCAGGGATGGTGGGTCACACGTTTC





TGTGGCTGCTCTACCCGCATCAGTGCCAGGCATGTGATGGGGGAGATCC





CAGGGAAGCTCCAGAGGCAGCGAAGACGAACGCGGTTGCTGGGAGGTTG





GAGTGTCTCAGAGGTGGCCCCCGGCAGTCGCGGCGGCTTTCCCACCGTA





TACAATACCTCTTGCTTTTCTCTCTCTCTCTCTCTTTCTCTCTCTCCCT





CTTCCCTGTCTCTCCTGCCCTCCCATTCCCTCTTCCTTCTCTCCTATCT





ATGTCTTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CYFIP2. In some embodiments, the sequences from gene CYFIP2 comprise or consist of:









(SEQ ID NO: 128)


TATCACACCGCCTGGCTGGGTTGGTTTGGGTACCCTTCTCGAGGAGGCA





GCAGGAATGGACTAGCCTGGCTCCTCAAGGCCCCTTCCAGCCCTAAGAT





TCACAGACAAATGATGTGGAGAGATCTAGATTAGCAAAGAGAACCACCC





CACCGCACTTGCATCCTTTGCACTTAAGGCACCCTGTAAGCGGGAGTTA





ATTGTCTAGTAGCCTCCTGTGTAAGTACCAATGTTAACCCGCCTTCCAC





CCTGAACTTCAGCAGGCTGTGCAGTGCAGCTGATGCCACCAAGGGGGCG





CCATCGCTCAGCCATGGGGTCAAAACGCTGAGGGTCAGGAACAGGTGTG





GGAGGGGGTTCGTTCTGATCTTATCCCATTTCTCAGTTCCTCAGAATGT





TAGTGCTGAAAGGGACTGGTGTGAGAAGGCATCTGGTCCAACCCCCTCA





TGTTACATGAGACAACTGAGGCCCAGAGTGGGGGCTTTGTTGCCTAAGG





TCACACAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PTTG1. In some embodiments, the sequences from gene PTTG1 comprise or consist of:









(SEQ ID NO: 129)


GCCAATTGGGCCGCGAGTTGTGGTTTAAACCAGGAGTGCGCCGCGTCCG





TTCACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCGTGAGTGAA





TGGGAGGGTCGCGGGTGGTTAGTTGAGCCGGCTCCGGCGGGGAAGGAGG





CGGGCTGCGGCTGCGGCTGGGGCTGAAGCTGGGGCTGGGGTTGGGGGAC





TGCCCGGGGCTTAGATGGCTCCGAGCCCGTTTGAGCGTGGTCTCGGACT





GCTAACTGGACCAACGGCAACTGTCTGATGAGTGCCAGCCCCAAACCGC





GCGCTGCTCGGGACCTTAGAGCCTCTGACTCAGGCTGGAAGATTTGAGA





GCTGGATTAAGTACTTGTTGGCTCACGCCCGTGACTGTTCCGCTGTTTA





GCTCTTGTTTTTTGTGTGGACACTCCTAGGATAGAAAGTTTGGTATGTT





GCTATACCTTTGCTTCTCCCACCTTCCCCAATATCTAATATGTATTTCT





CATTCTTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PTTG1. In some embodiments, the sequences from gene PTTG1 comprise or consist of:









(SEQ ID NO: 130)


TAAGTATACAAGGCTGCAGTCGGATACACTGGTATTGTGGACGTGGCCT





GGAGCTGGACGAGACATTTAGTGTACTTTTTGGGCAATTGGAGTCGTTT





GTTATTGGTCCTTTTTCATTTTTAATATCTTAATGAGATGATTTAAGGA





AGTTACTGAATCTCTGCTATTAGGCCTATCATAGCCATGCCACTACCAA





AAGTGGGGGGTGGGTTTGGGGGTGAGAGGTCAAGTTTATATACACAGTA





TTTAAGTTGTACAGGTATTTTGCTGCTGGAATATTCTTATGGTAAGACT





TTTTCTTCAGCAATCAAAGCCTTAGATGGGAGATCTCAAGTTTCAACAC





CACGTTTTGGCAAAACGTTCGATGCCCCACCAGCCTTACCTAAAGCTAC





TAGAAAGGCTTTGGGAACTGTCAACAGAGCTACAGAAAAGTCTGTAAAG





ACCAAGGGACCCCTCAAACAAAAACAGCCAAGCTTTTCTGCCAAAAAGG





TAAGTGTTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SQSTM1. In some embodiments, the sequences from gene SQSTM1 comprise or consist of:











(SEQ ID NO: 131)



GCCGCGTACCAGGACAGCGAGAGGAAGGCGCACAGGCAGAAGAGC







AGCAGCGTCAGGAAGGTGCCATTGCGGAGCCTCATCTCCTCGGGT







GCGCGGCGGGCGCCCGCGGGGCCGAGGCTGCATGGCCCGGGGGAC







CGGGGCCGGGGCGCAGGGGTCGGAAGGCGGCGGCGGCGGCGGCAG







GGGCCCCGGCCCCGGGTCGGGGAGGGGCGGGGGGCCCGGGGCCGG







GCGGGGACCGGGCCAGGGAGCGCGCCGGCCGCCCCTCAGGGCGCA







AGCTTTGTGCCCTGTACTCAGGGAAGAGGAACAGGCTCAGAAGGG







CAGAGGCAGGTATCAGGCTCACTGCAGATATCAGGGGCGCGGGAC







ACTGGCGGCCTCGCCTCCGCGGCAGGGCCGGGCCGGGCCGGGCTG







GGCTGGGCTGGGCGGCGAGAGCCGCGGCCCGGCCTGGATCTGGGG







CCTGGATCTGGGGCCGCCGCGGAGTCGACGGCGCAGGGCGGGGCG







GCCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SQSTM1. In some embodiments, the sequences from gene SQSTM1 comprise or consist of:











(SEQ ID NO: 132)



TAAGGCTGGAGTGGGAGTGCAGGTCGACCGCAGCCGGGGCGGGGG







GCGGGCGGCGGGGGCGGCGCTCCGGGACCCCGGTACCCTTCTCAG







CAACATCTCCTCCGCGCGCGGACCCCGACCCCATTCGCACGTTCT







CGGGGCTCTTTCCCGGGCGTGAGGGGCTCTGGGTGGCGCGGGGGA







CTGGGCGGTTGAAGCCGGGAGCGACCGCTGCCTTCGCTGCCGCCC







AGGGCGCTTCCCCGCTCAGGAGCTTCCTCTGGGCCTCTGGACGGA







GGCGCGCAGGGGCCCGGGAGGCGGGAACGATGGGCCTTTCTAGGC







GGTATCAGGGCCGATGCTCGTGGATGCAGAGCAGTGACCAGCCCA







GAACCGTACCGGCTTCCCGGGGCAGGGGCGGCCCGAGAGCGCCGC







TAACGGGAGCAACGGGCCCTGCCCCCGGTGGTGTAGGGGCCACCT







CGCCCCGCCCAGCCCAGCCCGATATTGATGGGGGCCCACGCCTTC







ATTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TUBB2B. In some embodiments, the sequences from gene TUBB2B comprise or consist of:











(SEQ ID NO: 133)



GTGAGTGCCTGGTGTGGCTGAGAGCATGAGGGATTCATTTTACGC







TGGGCAGTGGAGGCTGAAGAGGTGTGATTGCCAGAGGGAAAGCAT







GAAGAACATCCGCGGTGTGCCAACTTAGCTTTAATATAGTGTAAA







ATTGTTTGCCTTTCAGCTGCTAAGAGCTTGGTGTCCTGGCCTTCC







TTATTTATAATTATATTCATGAACAAATATTTTAACGTTTGGCCA







TTTTGGTGATAATCTGAAATAGTCTTATCTGAGCATTGACTCACT







GATCTATATTGAGGGGTTTGAGGTAAGGTATTTAGTACAATCACC







GGTGACTTAAGATTTCTCTTTCCCTCTGGCAGGCCAGAGTGGAGC







CGGGAATAACTGGGCCAAGGGCCACTACACAGAGGGAGCCGAGCT







GGTCGACTCGGTCCTGGATGTGGTGAGGAAGGAGTCAGAGAGCTG







TGACTGTCTCCAGGGCTTCCAGCTGACCCACTCTCTGGGGGGCGG







CACG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TUBB2B. In some embodiments, the sequences from gene TUBB2B comprise or consist of:











(SEQ ID NO: 134)



GAAATGTTGGGTTGTCGGAACTGAGTCGGGGACATTTCATTGTGA







GCCTTGGCGTTTCTGGGGGCATCGTTCATGGCTAAATGCAGCCTT







TCTGTCTCAGTTTTGGGAGGTCATCAGTGATGAGCATGGGATTGA







CCCCACTGGCAGTTACCATGGAGACAGTGATTTGCAGCTGGAGAG







AATCAATGTTTACTACAATGAAGCCACTGGTAATCACCCTTGCCC







CACCCCCACTCCCTTCAGTTTTTCCACCTTCTCCCCTTTCCCTTG







CGTGGGACCCCAGGGGTGTGGCCCTGGGAGAGGGTGGACTATTCA







GTTTAGGATCCCCGCTCTTTCCCTGCAGTATTTCATTGGGCTTCC







TTATGCCAACAGAGCCCTTTGAGAACCTGATGGTGGGTCTCCCTT







TGTTTGGGGCAACATCTGCATGATGGCAGCAGGCGCCAAGCCCTT







CTCTGCAGAGGTCCTTGCCTGAGGGTCTAAGTCACTGTTGTTCCT







TGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GABBR1. In some embodiments, the sequences from gene GABBR1 comprise or consist of:











(SEQ ID NO: 135)



GTGTGTGGGATGTGGGCAAAGGAGGGCAGGGATGCACAAAGGCAG







GAGGGAAGGCAGGGGTAGAGGGCTTGGAGGGAGAGGGGTCTTTGG







AAGAGGAGGTAGAGAGCTTGTCAACCCAGTTTGAACACCCTACTC







TTTGTTATTGCACTAATCTTTTCTGAGAATAGGGGAGAGTTGCTC







TTTTGCTATGAGGAGCTTAGGGCCCAAAGCACAGAAAGCACAGAT







GAAGAACTTGTGTTCAGCAGAGGAACAAGTGGGGGTAACCCCACC







TCCAGACTTGACATTATCTTTTAGATCCCCCTTGGCCTTATTAGC







ATTGTTCGATTCATGGTCACAAATTGCAAACCTACCCTCTGCCTG







GAAAGCCACCTTCCCACCTGTAGGGTAAGGGTGAGGCATGTGTGG







CCCAGACTGGCCTATTTCTAGATATTCAACAAGCCCTTGCCTGAC







TGACAGCAGCTTGCCACCATTGCTTTCCTGTGTGAATCCCAGGAA







AAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GABBR1. In some embodiments, the sequences from gene GABBR1 comprise or consist of:











(SEQ ID NO: 136)



GTATGCCACAGTGGGCCTGCTGGTGGGCATGGATGTCCTCACTCT







CGCCATCTGGCAGATCGTGGACCCTCTGCACCGGACCATTGAGGT







ACCACTGGAGAGGAGGTGCTATGGTCAGGAGAATGAGCAGGGCTC







AGTGGCCATCAGGGCCCTGGGGCTGTGTGTGTCTTGAGGGATGAA







GCTACTTGGAGAGAGTGCCTTCCTCGTATTGGAAGCTCTTCCTTT







CCTTCCTAGAAGGAGCCCCTCATAGGCCTCCAGATTCAGCTGAAG







AAAGGAAGGGGTGGGAATCTGGGAAGGGTGTGTAGAACTTCCAGG







CATCAGGGAAAGTGGGGAACAAGCACCTCCAAGGGTTCAGGAAAA







CATTCTTAGGCCTAGAATGAGATTTGGCATCAGCATTGAGGGTCT







CATAGGAAAACAGTTGGAAGCCAGAGACTGAGAAGCGTTGAGGAG







AGGAGGGGAGGCTGGCAACCATCTTTCTTGTGACCTTGTTTCTGC







CCTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RPP21. In some embodiments, the sequences from gene RPP21 comprise or consist of:











(SEQ ID NO: 137)



TTGAGGAAGACAAAAGCACTGAAAATTCATAAAGGGACCCTGGGC







ATGGATTGCTGGGGTTGTGTTTAGAAACCGATGAGTTTGTGAGGC







CTCCGGGAGGCTCCCGAGGGCGCGGGGACTACGTTTCCCAGGAGG







CCTCGCGCGGACGCCCGGGCGGGGCTGTGCGAGGGGTGGGGCTGC







GGGAGGCCCTGGAGCGCGGCGGTGATGGCGGGGCCGGTGAAGGAC







CGCGAGGCCTTCCAGAGGCTCAACTTCCTGTACCAGGTGAGTCTG







CGACAAGGGCCCCACGGGGACGGTGCTCGGCGTCCCAGAGTGACT







GCTCCCCTCCCGCAGGCCGCCCATTGTGTCCTTGCCCAGGACCCC







GAGAACCAGGCGCTGGCGAGGTTTTACTGCTACACTGAGAGGACC







ATTGCGAAGCGGCTCGTCTTGCGGCGGTGAGACAGCCACGGGGCG







GGCGGCGGGCGGGACGCGGGAGGAACGCGAGAGGGAGCGCGGGCG







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RPP21. In some embodiments, the sequences from gene RPP21 comprise or consist of:











(SEQ ID NO: 138)



GATCCCTCGGTGAAGAGGACTCTCTGTCGAGGCTGCTCTTCCCTC







CTCGTCCCGGGCCTCACCTGCACCCAGCGCCAGAGACGTGAGTGC







TCCAACGGAGGTGGAAGACTGCGGAGCATTGGGGGCGCGGAGGGG







GGCGGGGTGGGGGGCGGGCACTGGAGGCCAACAGCGCCTTTCTCA







CTGTAGATGGATGTTGGGTGTGGGATTCGCAGGAGTCTTCCTTCT







TCGGGTTTGGATTAAGTTCCTAACGCCACTTGCACAAACTAGGGT







TTGGGCTCGGCTGTTTTTTTTTTTTTTCTTCCAGTGTGGGCAATA







AATAATAACTTTTAAGAGGCAACCCCACCCATGCACAATAATAGA







TGTTGTTCGGCTTTGTGGAGGACGATTCCCATCACCATTCATTTA







TTAAGCAAATACTTATTTTCTAAAATGTGTCAGGTACTGTGCTAG







ATTCATTATTCTCATTGAAATTACGGTCTGATGGGACAGACTAAG







AAAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TCF19. In some embodiments, the sequences from gene TCF19 comprise or consist of:











(SEQ ID NO: 139)



GGAAAACTTGTGAACTAATCAGAAAAAGTGGAAGGCGGGAGATCT







TGGGGCGCTGTCCAATGGCGCGGAAGAGAACACATGAGCTGGCCA







ATCGGGAACGGCACGGGGGCGGGCTCGCTCGGCGCGAAGTTCGGG







CCCGGGAATTCCGAAGGAGGGGTAGGCGCTGCCCGCGCGCAGAGG







CCGCGCCCCTCCTGGCCCCGGCTTCTTGGCTGTCAAACAGATGCA







GCAACGTCGGCTCCTGCCGAGGAGCCCAAGGGGTCCCGGGATCCG







CCGCACAGGCTGGCACTGCTTGAAGAGGAGGCTACTCGGAGACTG







CGCCGCGCGGGTAGATCCGAAACGGGGCTGGGGCGGAGTGGGAAA







AGGCCGGGTATGCCTTGCATGATCGCGGGGAGCTCCTTCCTGTTT







TTATCCCACCTAGAGAAGCCGGGAAGTAGGGGTTTAGGTCCAATT







TGTTGGAGTACTTAAGGACTCGTTTGCACTTTCTTTTGGGGGATG







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TCF19. In some embodiments, the sequences from gene TCF19 comprise or consist of:











(SEQ ID NO: 140)



TTGGAGGCTCCCCAAATATTTTGCGATCTGAGGATCCAGCTCAAG







TGAGGTGCCATAGGACGTGTTCCTGAGTTTGCATTGCACGGAGAC







CTTCCTGGAATTTTTCATTTGCAAGTCGGCTTAACCAATTTTGCA







TTGAGTCCTAGGCTGCTTGCACTCTGAATTTGGGCTATTCAGGTA







GTGTGCTCAAAGTTGAAACCGCATACAGCACAACTCAAGTTTGCA







TCAGACTGGGAAGCGAACTTAAGCCAGCGGTGCGTGGCCCAGGAG







TGGGAAAGGAAATGGATGCCTGAAGTGGAAGAGGTGGTGCAGAGG







GGGCACCGCCCATGCTGCCCTGCTTCCAACTGCTGCGCATAGGGG







GCGGCAGGGGCGGTGATCTCTACACCTTCCACCCCCCCGCCGGGG







CTGGCTGCACCTATCGCTTGGGCCACAGGGCCGACCTGTGTGATG







TGGCCCTGCGGCCCCAGCAGGAGCCTGGCCTCATCTCTGGGATCC







ACGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ITPR3. In some embodiments, the sequences from gene ITPR3 comprise or consist of:











(SEQ ID NO: 141)



GCGCCGCTGCCCTGCTGGCCCACTCGCTGGCTGCACGTTCCTTCC







TCAGCAAGCGATTTCCTCAGGCACATGTGCTGTGCCAGGCACTGT







TTTGGGGCCTAGGGTCCAGTAGGGCACCAGACAGCAGGGCCGGGT







TCATGGGAATGGGACCTGGCCCGGTGCTCACAAGGGCCTTGCACT







GGGTTAGATGCTCTGCTGCTGAGCAAGGGTCCTGCTCTTTCATTC







TGCACTGGGCCCTACAAGTTATGTAGCCAATCCTTCCACACAAAC







AAGGTCCCTGCCCTCGTGGAGGTTTCGCTCTGGTGGGAAACAGCC







CACAAGCTAAATAACTATGTAATGTAAGCCACTCTTCTGCCCAGC







GATGCTTCCTTTCCTGGACCTCTGCCTTTCTAGGGTATTGGGTCC







TTACCTCGGGGTTTGGGGGCAGCGTTCCAGAGCAGAGCTGGGCCC







TTGGCCCACCTGTGACTCTCTGTGACCCCCAGCCTGTCTGCCCCC







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ITPR3. In some embodiments, the sequences from gene ITPR3 comprise or consist of:











(SEQ ID NO: 142)



TGGGCCCGAACCCCCTCCCCGGCCGGCGCCTGCTCCTCCCTCCTC







CCTTGCCTGGGTAGAAACTCTGCTGGTTGTGTGAACTATTCCTGC







ACCTGTTGGTAACTGCCACCCTGAATAGCACTGGGTGGCTCAGCA







CTGGGAGGCCCTAGCCTCCCTAGCCCTTCTCTGGGATCCCCTAAA







ATTTGGCCCACTCTGGCAACAATGGGAATGACTCCTTGTACCACT







AGAGGCCCCCAAGACCTTCTCCAGTGACAAAGTCAGAACCTGTAT







TGAACCAGTTGTTAAATATTTTGAATATCACCTCGGCCCTTGCGC







TTCCTCACCCTGTCCGTCTGAACCCCCATCTAGCCTTCCCGGACC







CCAGGGTGATGCCCTGGGGCTTTCCCTCCTTGGGGAGCTGGGAGT







CCATCCCACCCATCTCTAAGCCTTGGGAGGGAGCTAGTTCCAGGG







TGGCGGTGCTGCCAGCTGGGGAAGGGGAAGGGTGGGCTCTGTCCT







TAGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene HMGA1. In some embodiments, the sequences from gene HMGA1 comprise or consist of:











(SEQ ID NO: 143)



CCTGCAACGGCCGGGCGCAGACACTTGCAGAGTCACCCTCGGGCC







GGGTCTGGAGCCTGATGCCTCGGGGGTTTAGCCCTAGCCGCTAGG







CGGGTAGGCAAAGTGTCGGGTTGAAAGGGTCTCCGGGAACCTACT







TCTTCCTGCTCGCCAACTTGCTGTGTGACCTTAGGCAACTCAAGG







CTTCTCTGGGCTACTTTTTTACGGGGGACGGGGAAGAAAACAAGG







GAACCCCCAAGATAATTTCCTAGGGCCTTTCCTGCAGAGTCCTTA







AGTATTTGAGGAGCACAGTTTGCATGCACAGTGTATGGAGGAGAC







AGGCTTGAGGCCCAAGCGGGCTTATTACAAAGTGAGGGATTGGGG







TTAGGCCCTAAATGTAGTTCCTCCAAGACAGGCCTCTGATGTTCA







GAAACAGGTGGAAAAGACGAACGTTTCTTGGCCTCACTGGAAAGG







GCCTCGGGCCTTCATTTAGTTCGTGGGGGGTTGGCTGAGGGGATT







AAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene HMGA1. In some embodiments, the sequences from gene HMGA1 comprise or consist of:











(SEQ ID NO: 144)



TCAGCACTCGTTTAGTTGATTAATTTTGATGTTTAGTTTGAAGGG







GGACCTGTGGTGTAATATAAGATTCTAATCACTGCCTGTAATTGC







AGAGCAGGCCAAGCCACTAATGACGGAGCAGGATAAATCACCAAC







CACCTTGTTTAAAAGGGTCTCCAAGTCTGAAACATTATAAGATAG







AATAATTACCAAAGTCCTGCTGTCTGGAGGGTCTCTTGATGCTTG







AAAATGGAGGTCCTGGGCCCTCTAGAAATCACAAGTTTGCAAAAA







TGTTTTTTTCCTCTCATTGGGGTAAAAACTCTTTTTCTTCTTTTT







TTTTTTTCTTTTAAATTAGACATGGGAGTCCCACCGTATTGTCCA







GGCTGGTCTCGAACTCCTGACCTCAAGCAGTCCTCCTGCTTTGGC







ATCCCAAAGTGCTGGGATTACAGGCATGAGTCACCTCACCTGGCC







CATAAATCATTTTCAAATGGTCTAGTTCTTATTTTGGTTAGAGTG







AAGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene KLC4. In some embodiments, the sequences from gene KLC4 comprise or consist of:











(SEQ ID NO: 145)



TTTGGCTTTCATTTTTCCTCTTTACAGGGTACCTCTCCTCCCTGT







TGGGCCTCCCAGATACACCCCAACAGGGTGCTTCTTTATGTGCCA







GAACTCTCACTTTTCCCATACCTAAGCATTTGCTCATCCCTTGGG







ATCCTTGTTCCTCTAATCTCTCTTCTGGGGAGTGCTCTCTCCCTC







TCTTCCCCCTGCCCCCTTGCTTCTTATAACTTGTGGCCTCCTCTT







GGGATGGCAGTGCCCTGCATTCTGGTATGAGTATGTCAGTGAAGC







CCCCTTACTGTCCACGCCAGGCTTCCTGTCTCCTCATGGAGGTCT







CCAATCCTTAAATGTACTCAGTCCTTCCAAAGGGAAGGAGGGAAG







GAGAAGGCTTGCGGGTTCAGGGTAACTCCTGTCACTTTTGTCTTC







TTCCAGTGACCAGAATAAGTATAAGGAAGCTGCCCACCTGCTGAA







TGATGCCCTTAGCATCCGGGAGAGCACCTTGGGACCTGACCATCC







TGCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene KLC4. In some embodiments, the sequences from gene KLC4 comprise or consist of:











(SEQ ID NO: 146)



AGTAATTTGTAAGGACAATATATACAATGTAATATAATAAGGTAA







AAATACAAAACAACAGTAGTTGTATTGAGTACAACTTTGTTAAAA







GAAATAGCAAAGTGGCCTGGGCACAGTGGCTCACGAGTGTAATCC







CAGCACTAATCCCAGCACTTTGGGAGGCTGAGGAGGGTGGATCAC







GAGGTCAGGAGATCGAGACCATCCTGGCTAACATGGTGAAACCCC







ATCTCTACTAAAAAAAATACAAAAAATGCCGGGCGTGGTGGCGGG







CGCCTGTAGTCCTAGCTACTCGGGAGGCTAAGGCAGGAGAATGGC







GTGAACCCGGGAGGCGGAGCTTGCAGTGAGCTGAGATCGTGCCAT







TGCACTCCAGCCAGGGTGACAGAGCGAGACTCCGTCTCAAAAAAA







AAAAAAAAAAATGAAAATATAGGTGGACAGGCCGGGCGCGGTGGC







TCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGAT







CACG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DBNL. In some embodiments, the sequences from gene DBNL comprise or consist of:











(SEQ ID NO: 147)



AGTGAGCTCATGCAGCATCCACTCTCCTTGGTGCCCATTACAGAT







GGTCACACTGAGGCTCGGGTAAGTTAAGCCACAAGGCTAATGATC







GACTGGCTCTGGTGCCCGTCTTTGGCCATGTGCCTAAAACTCAGT







CCTGGGCAGGGGATTAGGCTGAAGTGGCAGCATAGGGCTGAGCGG







GCAGTGGCTCTCCCTGCAGAAGGAGGAGGAGAACCGTCGGCTGGA







GGAAAAGCGGCGGGCCGAGGAGGCACAGCGGCAGCTGGAGCAGGA







GCGCCGGGAGCGTGAGCTGCGTGAGGCTGCACGCCGGGAGCAGCG







CTATCAGGAGCAGGGTGGCGAGGCCAGCCCCCAGAGGTGAGCCAG







AGGTGGAGGCTGGCCTGGGGGACCCACCCTGAGGCATTGCTGGGC







ACCTGCTCCATCCCATGGAGGCGAGGAGGACTAAGGGGTGTGGCA







TGAGAAGCTGTGACTGTGCCTCAGCTGTGCCCCACCCGGCCCTTC







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DBNL. In some embodiments, the sequences from gene DBNL comprise or consist of:











(SEQ ID NO: 148)



ACGTGGGAGCAGCAGCAAGAAGTGGTTTCAAGGAACCGAAATGAG







CAGGTAAGATGGGGGTGCTCTACTTGTTTGGACCTGTCCTGGCCA







CACGCAGAAGTCCCTGATCTCGGATTGAGGGCCCAGCCCAGACCT







GGGCAGAGGCTGCCCTGCAGTCAGCTGGGGCAGGTTGGAATCTGG







GCACCTCAAGAGGTGGCAGTAGAGAGGAAAGCCAAAGGCGGAAGC







GTCGGGCTTGGACCACACCTGGTCCTGGGGGAGGCCCTGGGAGCC







CCTTGGCTTCTGTGTTTTACTTCCTTTTTTAACGTTACTTTTTAT







TTTTAAATGACTTCTCTCCTGAGAACATGTTTTGCCTCCTGGCCC







CACACTCACCTTTGAGGGGCTACTGGGCCGACAGCTGGAGGGGCT







GTGATCTGGGGAGAGGTGGTGAAGGTTTTGCCCACTGCAGGGGTC







AACATGTGCTTCCCTCCAGGAGTCTGCCGTGCACCCGAGGGAGAT







TTTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene POLD2. In some embodiments, the sequences from gene POLD2 comprise or consist of:











(SEQ ID NO: 149)



GGTGAGACATCGGTATCCAGGCACGATAAATTTCCAAGTGGACAC







AATGTCTGGTGTCAACTACAGCTGTTCTCCTTCTTTTCCCAGTAT







CCTTTGGGTGCAGTGAGACACCAGGAGAGCTGCTGCTTTGGGGGA







TGGACAGGGGCAGCAGGAATGCCTTTGTGTTTTCGCAGTGAACCT







CCTTGGCCTGGGCGAAGCTGTGTGGACCAAGCAAGTCAGGAGTGT







GGCCATGTTTTCTGAGCAGGCTGCCCAGAGGGCCCACACTCTACT







GTCCCCACCATCAGCCAACAATGCCACCTTTGCCCGGGTGCCAGT







GGCAACCTACACCAACTCCTCACAACCCTTCCGGCTAGGAGAGCG







CAGCTTTAGCCGGCAGTATGCCCACATTTATGCCACCCGCCTCAT







CCAAATGAGACCCTTCCTGGAGAACCGGGCCCAGCAGCACTGGGG







TAAGTGAGAGTTTGGGAAGGTGCTTCCCCCACAGCATCCCTGAAC







TTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene POLD2. In some embodiments, the sequences from gene POLD2 comprise or consist of:











(SEQ ID NO: 150)



TCAGCTCTCCTGGAGCTTATGGTCTAGTTGGAGCAGGCAGACAAT







ACATTTAAAATATACAGTTTGTTAGAAGGTAAATGTTGTAAACAA







CAATAACAGTTGAAGTACTGGGGAGAGTTGCAGTTGTAAATCAGA







TGGGCAGGGCACAAGGTAACATTTGAGTAAAGATGTAAGAACTTG







AAGGAGATGGGCAAGTGAGCTCTATAAGTATACGGGAGAGGGGCA







AGCAAGAGTTCAGAGGCCCCTTGCTGTGGGGAGGGATCCAAGGTG







GAGGAGTGGGAACCAGGAGGGGAGAGGACCAGTGGAGCAGATCTC







ATAGGCAGTTGTAAGGACTTGGGGCCTTATTCAATGAAATGAGGA







CACTTTGGAGAGTTTTGAACAGAGCAGTGACTGATTTATGTTTTG







GTTTTGGTTTAGTTCTATTATTATTTAATAATAGGCTTATTATTT







CACAGAAGTTTTATTTAATAAGGCAGACCTCTTGTCTGGAAATGA







GACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene OGDH. In some embodiments, the sequences from gene OGDH comprise or consist of:











(SEQ ID NO: 151)



TCTTTAAATTTGATTTATTTTAAATTGAGGTGGGATCTCACTGTG







TTGCCCAGGTTGGCCTCAAACTCCTAGGCTCAAGAGATCCTCCTA







CCTCAGCCTCCCAAGTAGCTGGAACTACAGGCATGTGCCACTGTA







CCCAGCACCTTAGGGAAATGTTTATGTTCTGAGCTGAGGCAGGCT







TCACATTGGAGTAAGAATTGCCCAGCATATCTCATTTCTGTTTAT







CCATGGAAACTGTATAAAGATCACAAGAAATGGATCCTTTCAGAC







TCCTAAGTTTATCATAACCTGAGTTTATCTCTTTTTTATAAAAGT







ACATTTATGCACAATAGTGCTTTTAAAATAAGTTATTCCAAGACT







TTTAAAAACTAATTTTGTCGTATGGGTTTATTGGTGCTGTGTTCC







TCTTTGGAAAAGGTTTGCATAACCTGTGACTCTTTTAACCCTCCC







CACAGCTCACTGGATCTGCTTAATGACTTGCTTGTGTTATTGCTT







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene OGDH. In some embodiments, the sequences from gene OGDH comprise or consist of:











(SEQ ID NO: 152)



TGAGAATTAAGCTGTAAATGCTAATTTTAATGTAATTTTACTTTT







TTTTTACCCCTTCCCTCTTTTTTTTTCTTCTGTCCTTTTGTGTGT







GTCCTTCCCTCTCATCGTTGGCCACTCATAGATACGAGGGCACCA







TGTAGCACAGCTGGACCCCCTGGGGATTTTGGATGCTGATCTGGA







CTCCTCCGTGCCCGCTGACATTATCTCATCCACAGACAAACTTGG







TGAGGGTCTGAGAGCAGTCAGCTGCGTTGCTTGAGCTCCTCTCGC







TGTGCCACGACCTGTGGTAGCCAGGATGTCCAGGCAGGAGGGAAA







GGCTCTTAAGTTTCCTTTCATTCTGATCACTTGAAATTTATCGGT







ATTGTAGCCTTAGTGTGTTTTGGGCAAGTTATTTTTATCTTACTT







TTTCAGCCAGATTGTCTTAAGTCTCAAAATTTGAAAATAAAATTT







TGGACAGAAAGGTTTGTGGCACAGGGAAAAAGCTTGTCCACCTGG







GGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RFC2. In some embodiments, the sequences from gene RFC2 comprise or consist of:











(SEQ ID NO: 153)



GGCCCTCCAGGAACCGGCAAGACCACAAGCATTCTGTGCTTGGCC







CGGGCCCTGCTGGGCCCAGCACTCAAAGATGCCATGTTGGAACTC







AATGCTTCAAATGACAGGTAGGTCTTAGAGTCAGACCTGTAGGGG







GGCGGGTGCTCTGCATTGAGAATTGTTACAGAAACAACCTTTCTC







AGCATTGGGTTGTATGCGAAGCCTTGCCAGCTATGTGATTTCCTC







CACCCCTTCCCAGTGAAAGAGCGTTGCTCACTTGTCAACTTGCCG







GTTTATTTTTTTTGTTTTCATGGAAATTTTCAAAAACTGTTCTTC







TCAGCCAGGCATGGTGGCTCACACCTGTAATTCCAGCACTTTGGG







AGGCCGAGGCAGGTAGATCACCTGAGGTCAGGAATTCGAGACCAT







CCTAGCCAACATGGTGAAACCCCGTCTCCACTAAAAATACAAAAC







TTAGCTGGGCGTGGTGCTGGGTGCCTGTAATCCCAGCTACTCAGG







AGGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RFC2. In some embodiments, the sequences from gene RFC2 comprise or consist of:











(SEQ ID NO: 154)



GCCTGTTGAGTGTCTCCATGTCTCATCCCACTGTCCGCTGAACTG







CTTTCTTGTGCACAGCGCTGCCTCCCTGGAGAGCCCCTTCCCATT







CCCCCGGCAAAGGGAAGTGTTTCACTCTAGTTGGGAGGCCTGGGG







TTGGTTGCTGTATTTTTTTTTATTTATTTATTTATTTTTTTTTCT







GAAACGGAGTCTCACTCTGTCACCCAGGCTAGAGTGCAGTGGTGC







AATCTCTGCTCACCGCAACCTCTCCCTCCCAGGTTCAAGCGATTC







TTGTACCTCAGCCTCCCGACTAGCTGGGATTACAGGTGCCTGCCA







TCACGCCCAGCTAAGTTTTGTATTTTTAGTAGAGACGGGGTTTCG







CCATGTTGGCCAGTCTGGTCTTGAACTCCTGACCTCAGGTGATCT







GCCCGCCTTGGCCTCCCAAAGTGCTGGGATTATAGGTGTGAGCCA







CTGTCTCCGGCCAGCTGTATTGATCTGTGTGTGCTGTGGGTGGAA







TGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NRCAM. In some embodiments, the sequences from gene NRCAM comprise or consist of:











(SEQ ID NO: 155)



GTAAGAAAGAAAGATTAAAATTGCTTTATAATGCTACATAATAAT







CCATTAAACTAAAAAGATCATGGGGGAAATCATGTAGATTGTAAT







ATTTTGTCTTCCCAGAGCCATGAGTTTGGACTTTCTCTTCAAATG







ACTTTTGATCCCTTTTTATATAATGGCAATCTAAACATTGTCAAT







GATCATAAGGCATTTGGAGGAGTAAATCAATCAGTTCTCTGAAAA







CATTAGTGAGCCTACTTTCTTAGACTCCACATAGGTTATGGATGC







TGAAGTAACACCTAAAACCAACTGGCAAGGAGAGAGTTGCAGGGA







AGGTTCATGGAGTTTTTCAAAGGAAGTAATGCATGAACTAAGTCT







TCATATTGTGATGTGTAACATTGCTTTCCCTATAAGGTAATGGTT







TATTCAATTTAGTGGGCCCATTAAATTTATATTTTCTTTACCTGA







CCATTTTCCCTTCAGTGACAAGAAAAAAAAAATCTGTGAGAGAAT







ACTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NRCAM. In some embodiments, the sequences from gene NRCAM comprise or consist of:











(SEQ ID NO: 156)



TTAACAGATTTCTAAAAAATACTGAAAATGGCAACAGCCTTGCAA







GGACAGTTTAGCCTCTAAAAGCAGTTCCTTTGAAGAGAAAATACT







CAATTAGATGTATACATTCTGTTCTGTTTAAAACTCAGTTTTGTA







TTTTTCTCATTATACTTAATGTAAAATGTATCCTGTTAACAGTGA







ATACTTTAATTGGTTTGGGAATTTGTTTTTATTGAGAATATATAG







TGAAAATAACTGTGAACACAGTGTTCACCAGGGGTTTTTAGCATT







TAATGTGATGATTATTCAGATTTCTCTACTTGAATTTTTTAATGA







ATGAAGATTTTTCAAAGTTAATTATTCATATAATTCCTGATTAAT







AATTGACAGATCCTAATTTGGGGCCACATGTAAAATATATGAAAG







CTCACATAACGTGGCAATTTTTTACTCTGAGAGACTGCAGCAGTT







CCCATCTCCCCTTAACCTATTGCCACCAACACCATTGATTTGTTT







TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MEST. In some embodiments, the sequences from gene MEST comprise or consist of:











(SEQ ID NO: 157)



TGGTACGTGCCTAGCAGTTTTTTAGAGGTTACCTAATAAAACAAG







CTAGGTTTTGTATCACACAAAAAATTATTTATAGAAAATATTTTC







TTGGGCTGGGTGAGGATGCTCACGTCTGTAATCCTAGCTTTTTGG







GAGGCTGAGGTGGGAGGACTGGTTGAGCCCAGGAGTTTGGCACCA







CTCTGGGCAACATAGTGAGAAAAATTTTTCTCTACAGATAATTTA







AAAGTTAGCCAAGTGTGGTGTTGCACACCTGTATGTAGTCTCAGC







TACTGAGGACACTGAGGTGGGAGGATCGCTTGGGCCAACGAGTTT







GAGGTTACAGTGAGCTATGACTGCACCACTGCACTCCAGCCTGAG







CAAGGGAGAGAGAGACCTTGCCTATTATAATAAAAAAAGGAAGAA







GAAAACATTTCTTTTCGGAGAGGAGTTAATTTTAGGGTGGGAGTA







GAACAGATGTGAGAGCTGTTAAGTATTTCATTCCTTTTCTCTTTT







CTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MEST. In some embodiments, the sequences from gene MEST comprise or consist of:











(SEQ ID NO: 158)



TGAGTCTCCCCGAGAGAAGTCTATGTTTTGTTAGTATCTCATCCT







GACAGTGGTAAACAGAATTGCCTGTTGAGGGCTATTGGCTCTAGC







CATTGTCTTTAACCTCGCTGGCTGTTCATCCAGGAAACAATAAAA







AGATTGAGTTGAGGAATCTTATTCTAACATAATTGTAGTATCAAG







ACATTCAGTTATACGTTCTCTTGATTTATACATAGTCTGTTTTCA







CTGTCATGACTGCTACATATTCCTTGGTGTACAAAAAGCTCTCAT







GTGAGTCAAAGGTTGAAAGTGGATATACCTACAAAGTGTTTGTTT







GTTTTAGTCTGATTGTAAAGCCAATTCATTGGTTTAGGCTGAGCA







GATGGCAAGGAGAGATTGAGAAGAGCTCATTGTGGAAAAGTGCCT







TCTCATTGGTTGCTATTCTCTCTAGGTACTTACTTGGAAGGTATC







AGAATATGAAATCTTGATTATGTCACATAAGAATGTTCCATACCT







TCCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PTN. In some embodiments, the sequences from gene PTN comprise or consist of:











(SEQ ID NO: 159)



GTAAGTGAGAGGAACGTACGATTTCTCGGAGGGCTCTTCGATCGA







GTTTGCCTTCACCCTGTGCCACTTTTCTGGGTGCCAGCAGATGAT







GGTGGTAGGGGTGAGAGAGGCTGCCTGGTGTCTTTGCTGATAGGA







CTGCTTTCAAAACTGACTTGCTCCAGAGGCCAAGGGAGGCATATT







TTTGGGGTTTCAGGTTAGGTTAGTTCGGGTGTGCTTAGGTAAGTG







AAAGGAAAGAAGTTTTTGTGATGTTAAGCATGTATTTGGACCGTG







TTCCAGCTACCAGCTGGAGAGCAAGACAGGGTGGGCTGGAGAGTG







CATCTTTGCTTTTTGTAAAACCTGGAGCTTTAGTAGGTGTGTCTC







TGGCTGGGTAGGTGCAAAGAAAGTAAGGCAGCCTGAACAAGCAGC







TCTCATTTTCCCAGCCTCTCTCTGATTCTCTGATTGCCCCAGAGC







CTGTCAGCCCATGACTCTTAGCTGGCATTTCTAGGTAAGCTGTGG







GGGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PTN. In some embodiments, the sequences from gene PTN comprise or consist of:











(SEQ ID NO: 160)



AAGGCACCAAGGAAACCAAAAAAGGAAAGAGCTCTCCCTAAAGGT







AAGTAGCACTTAATTGGGAACTTTGAGACGTCTGTCACTCTTTGT







GACAATTGAGAAAAAGTACTTTGTACATTTTGTGCTTTGCAGGTG







AGATGTGACTGACACCTTACTGTCTCTGTGTTTAGAGAGGGCTTT







CGTGGGTGGAAGAAGAGGCTGGTTTGAAGAGAGTGCTTTTCCATT







CTGAATATCATTAAAGGATTGTGGGGGGCCAAAAATGTAACCTAA







AATGCCATCAATTGTCCATTTAAAATATCCTATTTGGCAAAGTGG







CTTTGCACTCATCTGAAGAATAGATCATGGGTGGAGTTTGGAAAA







GAAATGTTGTGTTTCGTCTCTGCTAAAAACAAAACAAGGTACCAT







CTCATGGCCTCTTGGTTCAAATCCGTTTTCTTGCTCCGCAGGGAG







AGACTGCCCCTGGAGTATTCTGCTTTTAATAAGCTTCCCAATCAG







CTCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SSBP1. In some embodiments, the sequences from gene SSBP1 comprise or consist of:











(SEQ ID NO: 161)



TTAGAAACCTCATCACATAATTTCATTTCTTTCCAAACTTGAAAC







CTTCTAAACTTGAAACCTTCTAAACTTGAAACCTTCCAAACTCAC







CACTTGGCGGCGTTGATCCATCCGCCCGCGCACAAAGAGAAGATC







GCGGAACTTCAGAGTTAAGGGGAGCCGTTTCCCGCAGCGCTAGCC







GGCAGTATTTCCAAGGCGCAAGTTGCGGAGTTTCTGTTTCCTTTT







TCCTCTGGCGAGCTTTGCGTTCCCTGTGCGCCGGAAGTGATCCCC







TGCGTGGCTGGGCTGCTCGGGTTAGATCGTCAGGTGAGGGAGGAA







GGGATAGCCAGCGCGAAGGAAGTGCTGGAGTCGTGTGTTTTGGCT







GCGCGTGATCCTGCGTGGGTCGGGAGGTGTTTCTGTGTAGGTGTC







TGGCCCTTTCATCAGTCGTGCGGAGGACCGCGTGATTTCCTTCCA







GTTCTCCTCGGTTTTCAGGTGGTGGCGCCATCTTCGGTAAAGGGT







GTCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SSBP1. In some embodiments, the sequences from gene SSBP1 comprise or consist of:











(SEQ ID NO: 162)



TACGTTGTGGTCACAAAATTGGAGTGCATAAAAATTATGAATATA







GTTAGGAATTTAGGACTGGTGAGTTGTACTTTTGCCATAAGAGGT







AATGTTTTTTTCTTATTTTGTTTTTTTCCTTCAGGAAAAGCCTAA







AGATTAGACTGTAAGAAAAGAAAATAGAAGCCATGTTTCGAAGAC







CTGTATTACAGGTAGTCACTTGTCTGTATTAATACTGAGATGTAT







TACTATCAGCCACAGTGATCAGAAGACTTCTTTAGGCTTTTAACT







ACAGGGGCAAAAGACCTTTGAGCTCACCCACCCACCTCTATACTC







ATTTGATATGGAGGATTGGGGACGATTTTGCCAGTGTAAATTATG







TTCACTAAAACAAAAAAAAAGTTCAGGTTTATTCACACGTAATAG







GAATCATTAATTTTTAAAATGTGTGTGTGTTGCTTGAATTTAACC







AGTGAGAGGACATTAAAGGTCTTTAGTGACCTAAAGACTGTAGGA







ATCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CNTNAP2. In some embodiments, the sequences from gene CNTNAP2 comprise or consist of:











(SEQ ID NO: 163)



ATTTCCCTAAAATATCAGTTTCATAAATTGCAAACATATGACCAT







AGTCCCCACTGAGACCAGAGTAATATGTTAATTAATGTTGGCATT







GACCACCCAGAATAGGACAGAGCATCTCTCTCCCTGACTTATGTT







CGGCCAGCAGTAGCTTTGCTGTCTTCAGAAAGTCTAGTGATCCAC







AGTCACACCCACAACACACATACACACTTTACAACTCATGGTCAG







CCTATCACATCAGGCTGTCTGGCTGTCAATCCCATGGTGGAAGCT







GCTTGTTATAGTTAGACAGCCAACTGCTGCTCAGTAGTAGGTCTT







ACACTCCAAGTAGATCCGTAGTGGTGCATCCAGTCACTGAATCCA







TGCTCTGCTGCTGTGTGTGAGTTTAGCTTAGGCTCAGGCTGTGCT







TCAAAACTTGTAGAACTATACTTTCATCAGTGGTCTGACATGGAT







GTTCATTTTATTCTGTGTTTTCCTCAGAGCCTGTCTTTCTATTTT







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CNTNAP2. In some embodiments, the sequences from gene CNTNAP2 comprise or consist of:











(SEQ ID NO: 164)



TCAGTGAAACCTATTTGACATTTGTTCCTGAAACTTATTGCAATT







TCCAGAGTTAATGTTGTGCTTTGTTTGGTTTTGCTGGTGTTACAC







GCTCAGGATTAGGTTTTAATTCAGATCTTCAAGACGCTTACTTGG







TTGTAGTGTGTGCTGATTGAATAAAGAAAACTTGGAAAGTTGTTG







AGAGAGCAAGGCATGGGTGAAGGAGAAAATTGGTTGAGATTGGAA







AAGCAAAATCTATTCCTTACCAATGCTAGTTTATTTTTAATAGAT







AAATTGCTCTTCTTTTAAGTGAATAATTTATGTTTAAAAACAAGG







AATGTTTATTTTAAAAACACAATTGTATCTGGTATCTTTTCTCTT







TATCAACATTCGTGTACCTTTTACTATTAGTAACAAGATTATACT







AATTTTAACTTTAAGGAATATTCCACTGCTGGAATCAGAACTTCA







AAATGATCTTGCTAAAATTATGCAAATGGTTGGAAAAGAAAACGA







AAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PTPRN2. In some embodiments, the sequences from gene PTPRN2 comprise or consist of:











(SEQ ID NO: 165)



GTATGACGCGCGGCCCGCCGGCAGGGCAGTGCGCGCTGAGCGCGC







GGCACAGGGGAGCGAGGCTTCGGTGCTCCTGAGACGCCCCTGGCG







CCGAGGGCCGCGTCTGTGTCGGCGCCGCAGGGGGGAGGGGAAGGG







AAGCGAGAGGCGGGAAGACTCCGTTAACGGCACGTGTTAATCGTG







AGCGATTTTCTAGCTTCAGAGGTATTAAAATGTGAGGGGGAATAG







GCATCTTCGAACTGTGGAAATAGGGCCATTATTTTTGATAAGGTT







CTTCAGTTGACAAAGAGCCTAAAGTGTATGTGCCACAGGATATTG







ATGCATAAAGCTCGGATGTTCAGTTGGATTCTCATCTGGCTGCAT







GGGATGAAAGAGGTAGCGAGCGCAGGCCCAGATACCACCCTGCGT







GATCTTGCAGGGAAGCCCGGAGACACTTAGCCCGTGGCTGTCTGT







ATTTTTAGGGCTATTTTGAAACTGACCCTCTTTAAAAAATTCAGT







TTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PTPRN2. In some embodiments, the sequences from gene PTPRN2 comprise or consist of:











(SEQ ID NO: 166)



GAAGTGCCTGCGCTGGGGTTCACATCAGCGCGACTCCGTGAGCCT







TTTGCTTTTCCTGCTCTAGACCCGGGTGTGCGCAGCTGCGACGGA







GTCTCACTTAGAGTGGAACCACTGAGCAGAACATGTAGTCGTGTC







GCTGCTTTTGTTGAAAAGTGTAATTTTTCTCTGGTTCCTTTTTTT







GAGGGTTTATGTAACATGGACATGCAAAAGACAGAAAGTAGGATA







GTTTTGGATTGTAACCTTTTAAAAAATTATTTGACGCTCAAGCCC







CTAAATAACACTAAGCACACACACCCGCACGGAGCTAACTTCAGT







GCGTTAACACTGGGAAGGTGGGAGGGGGCCGCGAAGCCGAGGGAA







CCCACCTGCGCTGTAAAATGTGCCCTCTTCGCAGCGCCCCCTTCT







CTGGTCAGACGTGGCCCTGGGTTCCTCAGTGCCTGAGGTTCGGTT







CACAATGTCCACACCTGTCTCTGTCCCCTCCCTCCGCTCCTCCCT







GCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene FDFT1. In some embodiments, the sequences from gene FDFT1 comprise or consist of:











(SEQ ID NO: 167)



TAGAGAGGGGGCAGCTGAAGCTCCAGGAGTGTGTGGGGCTCCCTG







GGGCTGGATGGCGGTGGCGGGCAGGCGAGCTGGGCTGTGCTCGGG







TGTGTTACAGTAAAGACGCCCAGCTTGGCGCTGGCCCGGCCTTTT







CACGGTTTTAGGCTCTACAGAGAGCGGCTGCAGAGCTCACCCGGC







TGGCAGGAGCCACCGAGGCCGGACACGTGGGCGACTTATTGACCA







AGTGGGGAGGAAGCAGCCCCGCACTGCTCTCCCGACTGCGGACCA







CCGTTGGGCTCCTGCGCATCCTAAGCCCCACCGCCTCACCTCCAG







TCCCCACAGCGTTCGCGCTCCCAGCCGGGGTAAGCGGAAGAAAAC







AAAGGCCCGGCTCCATCAGGGCACCAATCCCGCTCGTCGGCCTCT







TTCTCGGCCTCCAATGAGCTTCTAGAGTGTTATCACGCCAGTCTC







CTTCCGCGACTGATTGGCCGGGGTCTTCCTAGTGTGAGCGGCCCT







GGCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene FDFT1. In some embodiments, the sequences from gene FDFT1 comprise or consist of:











(SEQ ID NO: 168)



TCCAGCCGGCCGGTGAGCGCCTGGGGACCGCAGAGGTGAGAGTCG







CGCCCGGGAGTCCGCCGCCTGCGCCAGGATGGAGTTCGTGAAATG







CCTTGGCCACCCCGAAGAGTTCTACAACCTGGTGCGCTTCCGGAT







CGGGGGCAAGCGGAAGGTGATGCCCAAGATGGACCAGGTGGGCCG







AGCCTCCCTGCTTGCCCGGGGCGGGGAAGGAGCTCGCTGGGCCGG







CCTCAGGGCCTGAGCGGCCGGGCCCGGATCTGGGGCAAGGGGCGC







GGCGAGCAGGGCCGACGCCTGGGTGTTCCCGTCCCCCTTTCCTCG







AGCCTTCCCCCTGTAGGGCCCGGGTGGACGCGGCCGTCCTGGCTG







ACCTGTCCCTGCCCCCGCAAGCCGCCCTGGGCATGAGCGACTTTT







GCGTGGTTCCCGGTGGTTGCGCTCCCCGTTTCGTCCCCTCCGTGA







GCATCGGCGCTTACCGGTATTTTAACCCGAGGGTTACACATCTGA







GGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DMTN. In some embodiments, the sequences from gene DMTN comprise or consist of:











(SEQ ID NO: 169)



CATGGTGGGGGACAGTGGGAATGGGGCCTTGAGGAATTGGAAGAG







AGCTGTCCCTTGCTCTGAGTTTTCTCAGGTGGAATGAGAGAGGGT







TTGGGACCAGGTGAAGGTGCTCACAGAGACGGCAGAGGTGTGGGG







CCCGCAGCCGCGTGCCCAGTGCAGCCAGCGCCCAGGGGCCTCTGA







ACCCTGCCGGAGCAACCCCTCTGGCCCTTCAGCACAAGGACTGTC







CCTGCCTTGCTGCCTGTCATTGTCCCGAGCTCCACACTGGGCTTC







CTGGAGTGGGGACTCTCCCCAGTGCTGCATACTCTTCAAGACTGG







CCTGCTCTCCAGCCCTGAATTAGGGTTCTCCTGGGGTCACCCCTA







AAGCCAATTCTAGAGCCTTATGAAAGGAAGTGGGGGGATGGGGTA







CTTGGTGGGGTTCACTGGGCACCCTAGCTCCTGAAGCTGTCCCCT







AACCCCCAGCCCCAGCACTGAGGACCCACCCTCAGGCCCCCTCCC







TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DMTN. In some embodiments, the sequences from gene DMTN comprise or consist of:











(SEQ ID NO: 170)



TACCGAGCACCCTCCCCTCCTTCTCCCCTCCGCCCTTTCCCCTTG







ACTCCCCCTACTCTGCCCTACATGCACTGGCTTCAATAGGAGGAG







GCACCCCTCCTCCCAGGCCCAGCGATTCTACCCATTCCTCATCAC







CCACCTCGTTCCAGGCCCCTGGCCACAGCTGTCCTTCCTGGCATC







AGCTCATTCCAACTCTACCTCTGGGGACCCTTGCACCAGAAGACA







GCTCTCCTGGGGTGGGGCCTGGTGGGGGAGGGGCAATACAGGGGA







GGAACCCAGGTCGGGGTGCGGCGGGGATCAGGAATTCTAAAGGGC







TGAGGTGTCAAAGCCCCAAGTACCTGGTCCGCTGGAGGGACCGAG







AGAGATCATCTAGGGCTGGAGTGGGGCTGGGGCTTCCCCTCTGTC







TCTCAGACTCTGATTAGAGGGAAGTAAGGGTGGGGTTGAAGGTAC







AGAAAGCCGAGAGGCTGGGCTGAGGCCGGGCTGGTTGTGGAGCGA







TGGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CCAR2. In some embodiments, the sequences from gene CCAR2 comprise or consist of:











(SEQ ID NO: 171)



TTTGAGCCCGGAACCAATGATCCGAAGCCTTCTTTCCTGATGGGA







TTCTTTAGCGAGGACACTACCGAGGCGCGACTTCCTGACGCGCAT







CCGATCCGGGAAGGCGCACTTCCGCTCCCCAGTTGCCGCGGGATG







GCAAGCCGGCGTGGGGGAAGGGAGAGGTCGGCGGAGACGAGGGAG







GGACCATATCCGGGAGAGCGGCAGCTTCCGACCAGTGGTCGCGCT







TCCGGAGAGGCGCTTCCGGTGGCGGCGGCAGCAGCGGCTGTGGTG







GTTCCGGGTGTCTTTGTCCCCCCGGTGTCGCTGCCCTGGCCCGCA







GGTGGGTTGGGGGGCCCCCTGCCGCCCCTCTGCCCCTTCGCCCCT







CCGCCCCTCCGCTGGCCGAGGGGCTGCGAGGGCCGGGCGGCGAAG







ATGCGTGGGGCGCGGAAGGGGCCGAGCCCCTCTTTGGACTGCAAG







TCCCGTCTCCCTGGCAACGGCCTCGGCCTTGGGGGCGGGCGGGAG







GAGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CCAR2. In some embodiments, the sequences from gene CCAR2 comprise or consist of:











(SEQ ID NO: 172)



TGAGTAGCAGTTTTGTCACTACCTGGTTATGTGGCCCCGGGCAAG







TCTCTTGATCTCTCTGAGCATCAGTTTCCTTGTCTGTAAAACAGC







ATGACAGTATCATGCACCACTAGGTTATTGTCAGGGCAAACTGGA







ACAATGTAATTTCTTGTTTCATTTCTTTCTTCTCTATATTCTCTC







CATCCATTGCTTTCATTCATTCTTATTACCCACCTCCCTGTTTTC







CGAAATATCCTTACTTAAGATTCTCCACTTTTCCGGGTATAGATG







GAAATTTCCCTTATAAGCTGTTAATTTCTTTCTTTTTGGATTGAA







GCCTTTTCCCCACGACTCTGAAAGAGGACAGCGTTCCCAATGTCC







CAGTTTAAGCGCCAGCGGATCAACCCGCTTCCAGGGGGACGCAAC







TTCTCAGGTGATCACTGTTCTCCCTACCTGGCCTCATCCTGGGAA







GTATGTGCTCTGGAGTTAGTTTGGGGTTTCCCTGGTGGAGAGCAA







TTTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GOLGA7. In some embodiments, the sequences from gene GOLGA7 comprise or consist of:











(SEQ ID NO: 173)



CCTGCTTTCTACTTCTGAACACAATGAGCAGTTTGTGTTTGAGGG







TTTGGGGAGGGGGTGTTGTTTTTTCTTTATGTTTTGTTTTTTGTT







TTGTGTATGAGTTGGGTGGCATAACCCACACGTAAGCTGCGACGT







CCAATCAAGAACGAAAATGACCCCTCACAATCAGTGATCGGGAAC







CACCTGCCACAATCACTAGGGAAGTGACACAGCCGGGAAACTCCG







CCCCTCACACCCCCGCGCGCTCGCGCCGGCCGCAGCCCCACAGCT







CGCCCCGGCCGCAGCCCCACAGCTCGCCCCGCCTCTCTCCTTCCG







GAAACAATCCCCGCGCGGGCGGAAGGCGGGGTCGGCCCTGGCCCC







GCCCACATCATTTCCCTTCCTCGGCCCAGCCCCACAGCGCGCAGC







TTCGGCAGGGTGGCTTTACGGCCGGAGGAGAGGGTGGGTCCCAGA







CCTGAGGTAGGAGGCCGAGCTGAGGGGCGGGGAAGAGGCTTTTTG







CGGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GOLGA7. In some embodiments, the sequences from gene GOLGA7 comprise or consist of:











(SEQ ID NO: 174)



TTTGTGTTTCCCGGGCCGAACCGGGTTGTGGGGGGCGCGGGGCCT







GGGCCAGGCGGCAGCTAAGGCCCGCGGTGACAGCATGGGTGAAGG







GGAGCGGGGCAGAGGAGGCCTTGGGCTGTTTTCGGCGGCGGGTGG







GGGCGAGGGGCTGGCGGGTCAGAGTCCCGGGTCCAGGCCGGGGCT







CTGACTCGCGGTTGGTGTTCCCCCGACCCCGCAGCGCGGGGTGTC







CTGTCCTCGCCATGAGGCCGCAGCAGGCGCCGGTGTCCGGAAAGG







TGTTCATTCAGCGAGACTACAGCAGTGGCACACGCTGCCAGTTCC







AGACCAAGTTCCCTGCGGAGCTGGAGAACCGGGTACGCAACCTGG







CTCCCCACGCCTGCTCTGGCGAGGGAGAGAGACTCACCGGGGACG







GGAAGGGTGTGGGCGACAACCGGGCCTTTGAGGGTCCCGCAGTTC







TGGGGAGGTCCAGGCACAGAAGGGGCCTTGGCCAAACGTGTAAGA







AGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TPD52. In some embodiments, the sequences from gene TPD52 comprise or consist of:











(SEQ ID NO: 175)



GTAAAGTATATTTTTATGAAATGTAGTTGGTCTTATGCTTTATTT







TTCTTTATTTAGATGTTTAACTTGAACTGTTCTGAATTAGGGAAC







TAGAATTCCCCTTGAAACAAGCTGTGTGTTTAACACTCTATCAGT







TTTCAGCTACACTTCCTCTGATGTTGGGATGGGTGCAAGAGATCC







GTTAAATGTTTTGATTTTGTTTTTAAAAAAATTATTTCTATTTTA







TTTTGTAAAACCTTTGTTTCCTGTCTGAAATCTGTTCACAAAGTG







AGCACTACTTGTTAACAAACCTTCTTTTGTCCCGCCACCTCTGTC







TGCATTCGCTTTAGTTACATTCTCCTTTTCTTCATCCTTCCCATT







TTCACTTCCCCCATCCCACTGTGACATTTGGAGCCAACTATGATT







TAATTCTACTATCTGCTTTCATTAGCACTTGCAAGTGATATTACC







TCACCTAACCTCTCTAGAGAGTTAGAGGATTTTTAACAATCTGTG







CTTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TPD52. In some embodiments, the sequences from gene TPD52 comprise or consist of:











(SEQ ID NO: 176)



GGTAACCTTGAACCTGAGTGGTCAGTGAACTGCATTGTCCTTGAG







TGGGCAGTTGCCTCCTTGTCTTACTAAGAGCCCTTCCCTGGCCTT







GCTTGTGTGTGTATGTGTGTATGTGTGTGTGTGCATGCTTCATTT







TTGAGGGGAGGCGATAAACACTGAATTAGACCCAAGTTCTATTCT







TGATTCTGCTGTGACCTACGGTAACTCATTTAACACCTATATGCC







TTACAGCTCCGGGTGAAAGCACTTTAGTTTTCAAAGTGCTGTAAA







AATGCAAGGTGGTATTGTTAGCTCTAGTAACTCCTGGGAATTAAT







TGGCTCCTGAATAAAAGTAAATTATGTTGGAATAACTTTGATCCC







CTCCACCTCAAAAAGTAGGTAATTCTTCATGGCGCCAAGAGAATA







AAGCTCCTGGGGAGCTAAGTTTTGTCAGCTTTTAATAATGTTGAA







GCACCACTATACTAACCTTATTTCTATCTCTTTTTAATGCTGCCT







CTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DCTN3. In some embodiments, the sequences from gene DCTN3 comprise or consist of:











(SEQ ID NO: 177)



GACCAGTGTGTGGAAATCACTGAGGAGTCCAAGGCTCTCCTGGAG







GAATACAACAAGACTGTATCCTTTCTGCTCAACTAGGACCCTAAT







GGGTGCTGTCCCTAAGCCCTGTCCTTTTGTTCCACCTCTAGCTCT







CTCTCATCCTATCCTACCCACCTATGTTTGCCTGTTCCCCACTTT







CCATGAACTTCCTCACTCAGATTCCTATTCTCACCTTGACCTACA







ACCAGACAATGCTTCTCTCCAAGCAATTCGTGCAGTGGGATGAGC







TACTTTGCCAGCTAGAGGCCGCCACGCAAGTGAAGCCAGCAGAGG







AGTGATAGCTGCTCCCCATCCCAAAGTGGGCCTGGGCAGTCAGGC







TCCAGGGCCCTATGCCAACCTGCCTTTGTTACAAGGCAGAGGAAG







CTTTGTATTTATTGGCTTCAAGGCCCACCTCTCTGTACTCTGGGC







TCTAAAGTTGGAGGTCAGGTTACCTGAGGTTTGCAATTTGCAACA







CCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DCTN3. In some embodiments, the sequences from gene DCTN3 comprise or consist of:











(SEQ ID NO: 178)



CAGCTTCAGGCCTTTCTGTTGTAACCTCAGCAGAAACTTTGGGGT







TTATCCTTTATCTTGCATATGCCCCAGGGAGGTAAAGGGACTTGC







CTAGGTTCATGTAC







AAACTTTCTGCTTCCAGAGCAGGACTTTATTGGGACACCATAAGG







TTGAGGCTGGGAGGTACCCAAGAATTCCTGGGGATGGTATGTCAT







TGACTGTGGGTAGAGGGGCTCTTTTTTCACTATTCCCAGGCCCTA







GTGTAAAGTTCAAAGAGAGTTCAGAAATAAGGACAGAAGAAGAGA







CATACACTAGGGGGAATGTTTACTTAATGTATGGCCACTCCCTTT







TCCATCCGTCTTTGGACTGCTGTTAGGGCACCCATCCTGGAGCCT







GAAAGTGCTCCCATAGCCATTTGCTCCTTTGGCTTTGGGGTTAAG







CGTCATCTTTTTTAGGCTCCATGGGGAGTGGGAGTCCGTGATGAG







GCAGGAAGTTTAGTGGAAGATGTGGGCTTTGCCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SPIN1. In some embodiments, the sequences from gene SPIN1 comprise or consist of:











(SEQ ID NO: 179)



TGGTGAAGACGTCTCTTCCAGGGAGTCACAGGAAGCAAATCCATC







CTTTTAAAGGGCTCGCGTCCCGCGGCCCTGAACCGGCGCGCTGGA







GCAGTAATGCGACGTTCTCCGAGGTTCAAGGCTCACGGCCCAGCC







CTGTGATCCAGTTTCCTCAGTCTTCGCGGTGTGCACCACTCAGGA







CGACTTGCGGCTTACGTCCAGGACAAAGGGGGCTTGTCCTTTCTT







CAACGTCCTACTGCGCAAAAACGAAGCCCTTCTCAGGCTGCCTGC







CTTCCAGGAAAAGCTGCGCCCCCTCAGGAACCTGTGCCCCGCGCG







CCTGCCGCCGCCCATTCCCGGCGCGCACCTGGCAGTCCCGGGCTC







GAGCCCCGCCCTGCCGCCAGCCAGGTGAGAGGGGCCAGGCCCCGC







CCTCGCCGGCCCCGCCCCGCCCCACTGCGCCTGCGCAGTCGGCCG







TCGGCGCCAGGCGCCGGGGCGGCGCGGCCGTCGCAGTTCGGCGGG







CGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SPIN1. In some embodiments, the sequences from gene SPIN1 comprise or consist of:











(SEQ ID NO: 180)



TGAGGCCCGCGCAGGGCGGGCGTGGGTCGTGAGGGAGTGCGGGCT







GCGGGCGGGCCAGCGGTGCGGCGGTCTGCCCGGGGGCGCAGGTGA







GCGCGGCCCGCGCGGGGCCCGGCGGAGCATTGTCCAGCGGGCGGC







GCCCGCATAGCGCCCCCTGCCGGCTGGGCCGCGGCGGGGACCCGG







GCTGGGCGGGCCTGGGCCTCGGGGCGGGCGCGCGCTCCTTTGTTC







GCCGGCCCCTACTCCTCCGTGCCCCCATCCCCGCCGCCCCCCGCC







AACATGGCCGCCACCCTCTCGTCCCCAGTTCCCCGGCCGCCGCCG







GCCCCTTAGCGCGCGCTTTGTTTGGAGCCGGGCGGGGAGGCCGGC







GGGCAGGGGGCGGCGGCGCTGCGCAGTCGGGCGCGGGGAGGGGGC







GCTGCGCCAGAATGCAGGCGGGGAGGGGGAACGTTGCATAATCCC







GGGCCGGCGACGGGGGCGGGGCGGGGGGAGGGGCGGGACCCCGGC







GCGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SEMA4D. In some embodiments, the sequences from gene SEMA4D comprise or consist of:











(SEQ ID NO: 181)



GTAACGGGCTGGGCTGCCCTTCCCCTCTGTTCGTGGGTGGGTGGT







TGCACAGCTCCTCCCTCCTGTCTGCCCCAGTGCTGTCTTCCCCAG







CACATGCAAGCCAAGCTGATCCTGCGTCTAAGGAAGTCTAGAAGG







TTTAAACTCACAATAATTTGTTTTCAGTGCCTTTCTAGGAGTGTT







GCTGAGTTTAAGTGGATGCACAGTTTAAGGGCTTAAATAGCCTTG







TTTCCCATTATTTTCACAGCAAAGATTTCTGAAGACGTCTTTCTA







GAAATGACTGGAAAGTTTCAAGAGGCATAAGATACAGGTAAAACC







ACTTTATTCATGATCTGTAATTTCAGTTGAGCAGAGTTGATAATG







TGTAGAGGCCTCAGTTGGATCTGGCAGGTGGTTGGTTGCTTGGTC







CCTTGGTCCCTGATATCCTGTGGGGCAAAGCTTCAGACTTGGCTG







CCATCCCTTGGGCCCGGCATGGGGAGGTGGGTGCTGGGCACCCAC







AGTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SEMA4D. In some embodiments, the sequences from gene SEMA4D comprise or consist of:











(SEQ ID NO: 182)



CAAAAAAAGAGCCGTGATCATATAAGCTCTGAAAGCAAAAGGCAA







ACAAAAAAGAAACCCTCCTCACCACAGAATATGCTGGAGTCTGAT







TCCAAGGTTTAACAATTACGTGGCTCCTGTGTTAATAGAATCATG







GAGGGATCAGCGTCGGCTCCTGAGCGTCTGCAGTCCTGCACGTGG







CTCTGGTTTTGCGATTCTGATTCTCTTGATCTAATTGTTGGTGCT







GCTACTGCACCCCACGATCCCTGTCTTGAGACTGATCTCCCACAC







TGCAAAGGAGTGACAGCCATGCTATGGAACCTTGCCACACCACGG







TGGACACCGTTCTGTTTGGCTGAAAACCAGCCATCCAAAAATAAA







GGAAGATGAACAGCTCTTTAGTGCTTGTAATTATGCCATATGTGT







TTCTGCTTTTTCCTTCTTTGCCTCATGAAGATGAATTTTAAAGGA







TACATATCTGGAGCTAATAGACAATTAGATAGTACTTTTTTGGTT







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GSN. In some embodiments, the sequences from gene GSN comprise or consist of:











(SEQ ID NO: 183)



TGCTTAATGGTCCACTTTAAGGACCAGGGATGCACTTGGCATTTT







CGTTACTTCATTCGGAATGAGCTGGGAATCGCTCTTCCTCCTTAT







TCCCTTCCGTGGGAGGGAATCTCCCCTGCTTTGCATGCAGCCTAT







GTCACTCTTCGAGGGCTTTGCCTCCTGAAGTCCGTTCTCTCCAGG







GCTGAAATCTGCCTCCGCTGGTGCTAGTTGAGCATTGCTCGCTTG







CCCGTCCCATCTGAGCTCTTTGGCCCCAGCCCAGCTCAGGAGCTG







CCCTGGGTCTCCTCCCCGACCCTAGGCCTCTTCAGATCTCCCCTC







CGTGCTCCGGGCCTCAGTTTCCTCCAGGGGAAAGCAGAGGGCTTG







GTTTGGTGCCTTCCTGCGAGGTGAAGCGAGGGGTCCCCCTGGACG







AAGGCGAGCTTGGAGCCACCCGCCCAGCAGCCGACGGGAGGGCCT







GGCGCTCCTCCCCCACCCGCCTGCCCGGCCGCCCCGCCCCGAGGG







CGGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GSN. In some embodiments, the sequences from gene GSN comprise or consist of:











(SEQ ID NO: 184)



TAGGGGAGGCGCGCGGCCCCGGCCCCGGGCATCTGGGGGTCCCGG







GCTGATTTGAGTCCTTCCTCTCCCTTCCTTCCCGGTCCTATCTCG







CCCGCGCCTGGAGCTCGGCCCGGCCGCGGGGGCATCGCTGGAAAA







GGGCTTCCTCGCTCACTTTTCCCGTCTTCCAAGGGACCAAAGAGT







GTGGATGGGAGTTGGGGGATGGGGAGACGCCGGAGCAGGAGAGGA







GAGGGGCCAGGCCTCCACCCCGTGATTCCACTGTGCTGCTAGCAC







CTGAGCTAATAACCTCGACCCCGCCCCCGGGATGGGGGAACTGGC







CCGGGGTCACCCCTGCACTTGGGTTGTCTGACCCGGGCATGCAGG







GGACCACCAGCTACGGTCTGGGCGGTGACTCTGCTGGGGAGTCCA







CTCAGAGGACCCTTGCCTCCTGGACGATACCTGTGCCTGCTCTCC







TCTCCAGCTGCTTCCTCTTTCTGGGGACTGTTAGAAAGTTCTTCC







TTAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene STXBP1. In some embodiments, the sequences from gene STXBP1 comprise or consist of:











(SEQ ID NO: 185)



GTCAGGTCACCAGCTCTCCACATAACTGTTTTTCCCTTCTGTTGT







GTAGCCCCGGTGATAAAGCTCTCTGCTCCCTGCTTGCATATGTAT







TGCATGAATGTAGTGAGTAATTCTGAGCCCAAACACAGCTTTTCT







GGACAGAAGCTGTCACAGCCAGGCTTTGGGTGTAAATCTTCCCCT







TCATCTTTAGACCTGACTTGAGGTTCCATATATCGGGAGGTGGGA







ACCTGGGGGTATGCAGTTGCCCATGAAGATTCATGGAGGGGCCTC







GCGCAGACAGCTGACGTCCGCTGTGTGGCCTCCCCTGCTTTGTGC







ATGCCAGCCTCCTTGCTACCATGTGTGTTCATGCGCGCTTGTGTG







TGTGTGTATGCATGCGCACACATGTGTGTGTGTCTTGCTTTCTCA







TACCTCATTGGCTGCATAAAATGTAGGCCTAAGTTGGCATGTTCC







TGTGACGTACCTTTGTTCAAGCAGTCAGCTGGCCTCTGTTCTCCC







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene STXBP1. In some embodiments, the sequences from gene STXBP1 comprise or consist of:











(SEQ ID NO: 186)



TAAAAGCAGCTTATTACAGAAAAGAAACTCTTCCACTCTGAAGGG







CTTTCTTTGATTATTCCGTCACTCTTTTTCTTTATTTCTTCCTTT







TTAGTTTATTATTTATTTGATTTTGTTTCAACAAGAAAATGCTTG







CAGTTCTCAACACCGATTGAAAATGTGTCCAGTGCTGCCCTCACA







GCCCTGCCATGAACCCTCTGGGCTCTGACCTGCTGGCTCACCCAT







GCCTTGGGTTCCGCACTGCTTAGTCAGACGCCTGCAGGCTGCTGG







GTGGAAACTAAAAATGATAATGACTGCTGTGAACAGTTGTCAGAG







AGGGAGCTGAAGTTTTGGGGGATGCCTGAGGCACAGTGCCCAACC







CTGCTGGTCTGGGATGAATTTGTGGCCCCCACAAGGAATGTAGAG







TGTCCTGGTTGTGCTGGGTGTGGGGGTCTGAGAGCGAGGCCAGTG







GACATTCAGGGAACACCATGTCCAGGAAGGCAGGCGGTGGTGGAG







ATGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene FAM102A. In some embodiments, the sequences from gene FAM102A comprise or consist of:











(SEQ ID NO: 187)



CTGGGCTTCGCTGACTTGAACCTGGCCGAGTTTGCGGGCTCGGGC







TCCACGGTGCGCTGCTGCCTGCTCGAGGGATATGACACGAAGAAC







ACTCGCCAGGACAACTCCATCCTTAAGGTACCAGGGATCCTGCCA







CCTCTGCCACCCTGACCACGGGATGGGGACAAACCAGGCTGCTCA







TCAGAATCCTCTGGAGGAGGGTTGTTAAAAATACAGGCCTTACCT







GAATAGGTAGACATCGGGAAGGGGCGAGCCCTGGAATCAGGAAAC







CACTATCAGAGGGAGGCCTGGCTCCGCTGTTGGCCTCAGTTTCCC







CATCAAGTTCAGAGAGGGGGCCCTGACAGTCTCCAGGGTTTGGCA







AGCACCTGCAGTGGGTTCACGTCATGCTGATTGCATGCCCTCTCT







TCTCTTGTGGTCCCCTGACAGCAGCCCCACGAGCTGTGGTATCTT







TGATTAGTCCCAGCTTCAAATGGGAGGGAGTTAAGGCACAGACGT







TAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene FAM102A. In some embodiments, the sequences from gene FAM102A comprise or consist of:











(SEQ ID NO: 188)



ACCACCTCGTCTCAATCTCACTTTCCCCTCCAGGTCTAGCAGACT







TGGCCTCCATCCACAGGGCCTTCTTAGCGCATGCCCTCTTCGCAC







TTACTCCCACTTCCCTCCCCATCCCTCGATCCTCTGTCCAGAGGT







CCCAAGGGCCTGTTCAGACCCTGAACTCTGCCCAGCAGCCCCTCA







CTCTGTCCTCTACCAGGGCGCCTTCCCTGGGGCTCCTGCTCATCC







TGCAGTGCCCCTCCCCTCCCAACCCCTGTGGGGGTGTCCCATCAC







TTTCCCCAGCACCCACTTCCCACAGGCCTTCCCTCCTCCCCTGCC







TGCTGACCCGGCAGCTCCCGACAGCACGGCCTTGTTTCCCAGGGA







ACAATGCATCTGTGTCACGGACAACAATGTCCTTTCATGTCAGGC







GCACTGGCCCCGGTGCACGCCGGGACGTGGCACAAAGGGTGCTTT







GTGCAGCTCAGGGATGTGAGTTCCTGGCTTTGCCATGTTGGTGCT







GCCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PTGES2. In some embodiments, the sequences from gene PTGES2 comprise or consist of:











(SEQ ID NO: 189)



GTACGTGTTACAGACGGCCACGCCGCCCTTTAGGCGGTCAAGGTG







GGGCGAGGAGACGTTCGCCCCCCTGCAGTCGGCCGGGTCACTACC







CAAGAGCCTTTGGAGGCGGAAGCATGGAACGGTCTGCAAACGTTC







CCGAGCGGGCCTCTGCGGCTCTGGCGGGCGTTTCGAACTTGGGCG







CCGGGCACACGCCCAGTCCCGAGAGCGCTGAGGGTTCCCTTAGCG







TCGCCCTCACCCCGGCCAACCCGCGGGGCGCCAGAGTCCTGGCCC







TTTAAACGCCGCGCGTGCCTCGGCGTCTTCGTTTCGCGCGCCCGC







CCGCGGCGCCGGCGGAGCGAACATGGACCCGGCTGCGCGGGTGGT







GCGGGCGCTGTGGCCTGGTGGGTGCGCCTTGGCCTGGAGGCTGGG







AGGCCGCCCCCAGCCGCTGCTACCCACGCAGAGCCGGGCTGGCTT







CGCGGGGGCGGCGGGCGGCCCGAGCCCCGTGGCTGCAGCTCGTAA







GGGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PTGES2. In some embodiments, the sequences from gene PTGES2 comprise or consist of:











(SEQ ID NO: 190)



CCGAAGCCGTCGTCCTCGCCTTCCGCTCTCGTCTTCACCGGCATA







CTCAGGTCCCCGCTGGGGACTGACACTGCGGGCTCGGTCGCCGCA







AGGACTGGAAGGAAGGTGGAAGATGCGGGGCGAAAGCGGAGCGAG







AAGAGTGGGACGCGGCGGAGCAGCAGAGCCGGGACTCAGAGCTCC







CCCCTCGCCCCGCCCCTCTCCCCGCCCACTCTGCGAGTCCCGCCC







CCTCTCGGGCGCCGGGCGGGGCCATCCCGGGGCTGTCCGCGGAGA







CGCCTATGCGGTGGAGGCTCCGGGCTTCAGCTAGGGCGGGGGAGC







CCAGCAGAAGGACCGAGCAGCCTGGCACCCCACTTTGCCATCCTC







TCCCTGGAAATCTCGGGGTCGGCGGGCCGGCCGCTTCGCGTGGGC







GAAATCAGAGACACGTGGTTTCCAAGGCCCCTTCGGGTTCGGGAA







AATTTTATGGTTCGGGTCACAGTAGGAAGCGGACAATGAGGCGGG







AGGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DNM1. In some embodiments, the sequences from gene DNM1 comprise or consist of:











(SEQ ID NO: 191)



ATGAAGCAAATTCTGGGACACTGGGAAACGTAGAATTGAACAGGA







TGGGTGGATGAGGTCCTGGCCTTGTCCTGGCCTGGGTCAGCTCCT







TAGGAGAACCCAAGGCTGTGGACAAAGACGGCACTGGGAGAGAGC







AGGCAGCCTCAGTGAGGCCGAGGAGCAGGGGAGCATCCCTGGGAA







GAGGATCTGAACTCAATAGGGGTCTTGTACGGAGCAGGGGCTTGG







TCCCTCGTACCTCTGGCCATACCTATGGAGCCCAGGGGATGCTTG







GCAGCACCTGGGAGGTGCCAACCCCGGGTGGCAAGGGAGGGCCGG







TCCCACGCTCACATTGTCTTCTGTTCTCTCTCTCTCTTTATCTGT







GTCGATGTCTCTCTCTCTTCCCCCGTGCCCGTGCCATCCTCTCCA







CCCCTGGATTCCTGTCTCTGCTTGGCTTTCACCCACTTCTCCTCC







CCACCCACGGCTGCTCCTCCTCCTGTCCCCACCTCCTCCCCGGGT







GCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DNM1. In some embodiments, the sequences from gene DNM1 comprise or consist of:











(SEQ ID NO: 192)



TATGACGGCCGCCTGGGCGGGGCTGGGCCTGGCCGTCCATTCCTT







GTGGCCACAGCCTCCCGTGGGCAGAAGGATCTGCTGAGCCGGCCT







CACGGCTACCCGCAGGGACCCAGCCCTAGTGTTTCCTGCCAGTTT







CTAACCCTGGGTACTTGCACTCATGACCCCTCCAGGCCCCCATCC







CAGAAGACTTGACTCCAACCCAAGCCTCCTTGGTGGCACCTATGC







TAGTGATGAAGATGATGTTAAGGAGATGGCAGCTGTTTACTGAGC







ACCTACTATGTGCCAAGCACACGCTAAGTGCTTGCCCTTACTATC







TGACTCAGTCCTCTCAACCACCCTAAGACGTGGGTAGTGTTGTTA







TTCCCATTTTGCAGATGGCAAAACAGAGTCTCAGAAAAGAGAAGC







AGAGTGTGATTCAGTTTTAGGAAGGACAGAGGAAGGGGTCTGAGG







TCAGGGCCTCCTGGGCAGGGGGAGCTGTCCTAGTTCCTCAAAACC







AATT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene URM1. In some embodiments, the sequences from gene URM1 comprise or consist of:











(SEQ ID NO: 193)



CTGGAGAGATAAAGGTGGAGGGGGCTCTTCAAGGTCCTCTCAGAA







GTCCAGCCATGGCAGTTGGAGACTGGCTTCCAGGACTTGTGGGTA







GGGGCCTGTCTCCTAACCCTTTAGATGCGACAGTCCTCCCGCCGT







CTGCCTTGAATCCCCCCACTATAGGTAAATCCCATTGCCTCTTCT







CCAGGACTTCAGCAGTGCCAGGTGCAGCTTTCTCTGAAGATCCAC







CTGGCAAGTCACTGGCCTGTCCTAACCTGTTTCCTCACCTTTGGA







ATCTACAAGTTCATGGGCTATCACAGCCTGGTCTAAAAGAAGCCC







TCTAAACACCGTGTGTATTTCCTTGTGGGCCAGGCAAGGCTCTGG







GGGTGGGCAGGTGCTATTTGGGTTTGACAAGAGTTTGTTCTGTGC







ATTCCTTTCCAGGGGACATCCGGAACCTGCTCATCTGGATCAAGA







AGAATTTGCTAAAAGAGCGGCCAGAGTTGTTCATCCAGGGAGACA







GCGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene URM1. In some embodiments, the sequences from gene URM1 comprise or consist of:











(SEQ ID NO: 194)



CGGCCAGGAATTCTGGTGCTGATTAACGATGCCGACTGGGAGCTA







CTGGTCAGTACCTTGGGGGACATCCCTCCCCCAGCCCCTGCCCTT







GCTGCTTCAGTGGGAAAGCGTTGGGCCTCTCCTCAGGCACATATA







GAGTGGCTGGGTAATCCTCCGCCCCACTCCAGCCCCACACTGAGG







CTGCTGGAGTCTCCCACTCCAGGTGAGGAAGGGATGGGTAGCTGG







GGACATGGGAGTACTCCTCCATCCTGAAGATTTGCAGGTTGAGGT







ATAGGATACCCATTTTTCTGGTAGAGTCTGTCTCTTCCAGAGCAG







CCAGTCCTCAGCCCCTGTTCTCCCAACTCCTGGGGTGGACCTGGG







AAGGTGGCCCTGAGGGTCTCCCGCTCCCCTCTCTCCCGCACCAGG







GTGAGCTGGACTACCAGCTTCAGGACCAGGACAGCGTCCTCTTCA







TCTCCACTCTGCACGGCGGCTGAGGGCCCTTCTCTGGGCCTGGGC







ACCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SH3GLB2. In some embodiments, the sequences from gene SH3GLB2 comprise or consist of:











(SEQ ID NO: 195)



GTGAGTAGCCGGGGCTGGAGTGGGGCTCCTGGGGCTGGGGAGGGG







AGTTTGCCTCTGGGGTCCCTTCTGGGACTCCCACCTGCTAAGATG







TATCCCTCCTCTCCAGATTTCCCGGCACCTTCGTGGGCACCACAG







AGCCCGCCTCCCCACCCCTGAGCAGCACCTCACCCACCACTGCTG







CGGCCACTATGCCTGTGGTGCCCTCTGTGGCCAGCCTGGCCCCTC







CGGGGGAGGCCTCGCTCTGCCTGGAAGAGGTGGCCCCCCCTGCCA







GTGGGACCCGCAAAGCTCGGGTGCTCTATGACTACGAGGCAGCCG







ACAGCAGTGAGCTGGCCCTGCTGGCTGATGAGGTGGGCCCGGTGC







CGCAAGGTGGGGCAGGAATGGGCTGGGTGAGGAGCAGCCTGGGCA







CTGGGATCAGGCACATGGGGCCTGGAGGGGCCCTGTTGGCAAAGT







GGGGACCAGCAGGTTAAAGAGGGCATATCCAGCTCTGAGGAAAGG







ATCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SH3GLB2. In some embodiments, the sequences from gene SH3GLB2 comprise or consist of:











(SEQ ID NO: 196)



GCCGGAGCAAGGTCTGGTAGAGGACGGGGATGGGGACGGAATTTT







TTGTTCTGAGGGCGGGAGGTTAGAAGTCAGCCTCTGGCCTCTTCC







CCGCAGGTGAACCACCTGCGCTGCCTCCACGAGTTCGTCAAGTCT







CAGACAACCTACTACGCACAGTGCTACCGCCACATGCTGGACTTG







CAGAAGCAGCTGGGCAGGTGCGGGGCCAGCACTGCTGAACCCACT







GGGGCCCCCTGGCTCCCATACCCTGTCCGTGGGCAGCATGAGCTC







CTCTGGGCAAGTCCTGTGACACAAAGCTGGGTTGGAGGGCTCTGC







TGGAACCCACTCTGGGTGCGGGCATCTCATGTGTATGAGGGTGGA







GTGGCTGGCCTTACTGACGGCCAAGTCCCCTGGAGCCCCTGAGGG







TTTCTAGGGAATGCCTGGGTGGGGAGGATGGCAGGGACCAGGGGG







TGGGGCCATCTCACCCTTCCCTTGCTTTGCTTTTCCCTTTGTGCT







GCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene AIF1L. In some embodiments, the sequences from gene AIF1L comprise or consist of:











(SEQ ID NO: 197)



CTAAGCCCCTCCAGCCTCTGTCATGCGTGGGCACACACTTTTGCA







AAGCTGCCTGATCGTTGCAGGGGTGAGGATGGAGAAAATAATGGA







CCGAATGGCAGGCCTGTCCATTCATTCAATGGATGTTTTGTTGTT







GTTGTTGTTGCTTTTTTTTTTTTTTTAGAGACAGGGTCTGGCTGG







AGTGCAGTGGTGCAGTCAAAGCTCATTGCAGCCTTCACCTCCTGG







ATTCAAGTGATCCTCCAGCCTCAGCCTCCCAAGTAGCTGGGACTA







CAGGCACGCACCACCACACCCAGCTAATTTTTTAATTTTTTGTAG







AGATGGGATCTCCCTATGTTGCTCAGGCTGACTTCAAACTTCTGG







CCTCAAGTGATCCTCCCTCCTCGGGCTCCTCAACAGATGTTTATT







GAGTGTCTACGATATGCTGGGCACTTCGACGCTAAACCTGTGGCT







GGGGCCCTGCCTCCTCCGAGCTGCCTCCTGATACCCACTTCCTCC







TCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene AIF1L. In some embodiments, the sequences from gene AIF1L comprise or consist of:











(SEQ ID NO: 198)



CAAGTTAGTTAACCTGAGTCTCAACTCCTCCACTCATCCTGCACA







TGTTTATTGAGCGTCTGCTATGTGGAACCTACATTCTAGCCGGGG







AGGCAGAGAGGGTGTAGTAACCTCAGTAAGCAGTGGCAGTGGAAA







TCATGTTTGGAGGTGACCCATCCTAAGGAGCTGGATAAGGGCGAT







TGGAAGTCGGGGCAGCTGAAGTGGGCAGGCAGCAGTATAAAACAG







GGTGGACAGGGCTGGGCATGGTGGCTCACACCTATAATCCTAGCA







CTTTGGGAGGCCGAGGCAGGCAGATCACTTGAGCCCAGGAGTTTA







AGACCAGCCTGGGCAACAGAGGGAGACCCCATCTCTACAAAAAAT







ACAAACATTAGCTGGGTGTGGTGGCATACACCCGTAGTTCTAGCT







GCTCTGGAGGGCTGAGGTGGGAGGATTGTTTGAGCCCAGGCAGGT







CGAGGCTGCAGTGAGCTGTGATCGTGCCAACAAAGAATAAATAGG







GTCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SURF4. In some embodiments, the sequences from gene SURF4 comprise or consist of:











(SEQ ID NO: 199)



GTGAGTTGTGTAATTATTTCATTATGTATTACAACATAATAATAG







AAATGAACTGCACACTAAGTGTAATGTGCTTGAATCATCCCAAAA







CCATCCTCCCGCCTCCATCTGTGGAAAAATTGTCTTCCACAAAAC







TGGTCCCTTGTGCCAAAAAGGTTGGGGACTGCTATTTTTTTTTTT







CTTTCTGAGACAGAGTTTTGCTCTTGTTGCCCAGGCTGGAGTCCA







ATGGCGCTATCTTGGCTCACCACAACCTCTGCCTCCTGGGTTCAA







GCGATTCTCCAGCCTCAGCCTCCCGAGTAGCTGTGATTACAGGCA







TGCGCCACCACACCTGGCTAATTTTGTATTTTTAGTAGAGACAGT







TTCTCCATGTTGGTCAGGCTGGTCTTGAACTCCCGATCTCAGGTG







ATCCGCCCGCCTTGGCCTCCCAAAGTGTTGGGGATTACAGGCGTG







AGCCACCGAGCCCGGCCAGGGACTGCTGTTCTAAGCCACAGCGGG







CTGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SURF4. In some embodiments, the sequences from gene SURF4 comprise or consist of:











(SEQ ID NO: 200)



TAGAGCCTGTGATTTGAGCCAAATCTGGAGAGATGGCCACCAAGC







CAGAACTCTGTGCAGAGACCAAGTGCTATGGGGCAGGGGGCTGGG







CCGGGGATTGCTGGAGGCTGACATTCATCAGAGGATTGGGGGAGG







GAGGGCAGGACCCAGAGGAGGAGATATGAGGATGGAAGCCTGAGC







CTAGGGACTCTCGGAGCCCATAAAAGTCTTAGGAGTCTGTAATCC







AGCCTTGTGAGCAGCCTGCTCATGACACTGGATATTCTAAGCCAG







GGGTCCCCAACCTAGTCCGTGGCCTGTTAGGAACCAGGCTGCACA







GCAGGAGGTGAGCAGCAGGCAGTGGAGCAAAGCTTCATCTGTATT







TACAGCCGCTCCCCATGGCTCGCATTACCACCCAAGCTCCACCTC







CTGTCAGCTCAGCAGCAGCATTAGATTCTCATGGGAGTGTGAACC







CTATTGTGAACAGTGCATGTGAGGGATCCAGGTTGCATGCTCCTT







ATGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ABCA2. In some embodiments, the sequences from gene ABCA2 comprise or consist of:











(SEQ ID NO: 201)



GCCAGCCTTCTACACAGCGGCGCCCCTGACGTCTGCCGGCATCCT







GCCTGTCATGCAATCGCTGTGCCCGGACGGCCAGCGAGACGAGTT







CGGCTTCCTGCAGTACGCCAACTCCACGTGAGTGCCCACCCGCCC







CTCGGAGCACCCACCCCTCCCCAGCCCATCCAGTGAGTGACATCC







GCCATGGTGCAGGGTCACGCAGCTGCTTGAGCGCCTGGACCGCGT







GGTGGAGGAAGGCAACCTGTTTGACCCAGCGCGGCCCAGCCTGGG







CTCAGAGCTCGAGGCCCTACGCCAGCATCTGGAGGCCCTCAGTGC







GGGCCCGGGCACCTCGGGGAGCCACCTGGACAGATCCACAGGTGT







GCCCTGTGCAGGGAAGTTGGGGGGCAGGGCTGCGGGCAAAGCTCT







GCTGTCACTGGGCTGAAGCCCACATCCACTCTGGGCCCCTCCACC







TGCACTGAGTTCCCAGCCACGCACAGGCTGCAGTGGCAGAGCTGT







GGTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ABCA2. In some embodiments, the sequences from gene ABCA2 comprise or consist of:











(SEQ ID NO: 202)



CGGCTTCTGGGCTGAGTCTGGGCTGAGGCTGGGCTGGCCTGGGCT







GGGGCTGGAGCGGCCTGGCTACTCTCCCTTCATACTCCTTCAGGC







TCTTGCACTCACTCTGCTAACCAGCTGGCCAGAGGTCAGAGGTCA







CTGCCCAGAGGGGGTCCTTTGTCCTCCCCTGGGCTCTGTAGGCAG







TGCCACACCTGAAACGTTTTCGGGGGGAGAGTGCCAGGGCATGTG







GGCCTGCACTGGCTGAAAGGAAAGGAATGCTCTGGGAGTTATGGC







AGAAGGGGTGGTGAGTGGCTCTTTGACCTCTGACCTGCTGGAGTC







ATTCGCCCCAGAACGCTTGGCCTGTAGCCCAGCTGGGGCCAGGGC







TCTGTGCCGCCTTCTCCCTAACGCTGCTTCTCCTTCCTGACTTTC







TCTAAATTTCTCCTCTCGGCCTCTGTCTCCCTCCCCTTCTGGATT







CCTCTCTCTCTCTCTCCCCTTTCACCCCCGACCCTGCCCTCTCCC







GTGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GPM6B. In some embodiments, the sequences from gene GPM6B comprise or consist of:











(SEQ ID NO: 203)



GTACTTCCTTCTAATTCACCCCTCCCCCAGTTATTTCATTATCCT







CTCGTGCATATGTCTGGACCTAATTATTACATGCGCATCCTTTTA







ATTGTCATGATCTGTTTCATCCCTTCTATTAGAGTAATTTTATAC







TTTTCCCTTCAATATGCTATTTTCTGGGTGGAAACTTCAGGGAGG







CTTTTAGCTTGCTAGGTATCAAAATTTCGTTATATTTTCATGTTA







AGTGTGTAGTGAAAGAAGCTTTTCTTAATATGAAACTACTTTATA







ATGCAGTTCAATCAAAAAAGACTTCTGGATCTCCGCTACAAAAAG







ATTGTATTTTTTCTTTATTTTCAGTTTTATAACATCCAACTTTCC







ATTCAAAATAATATTGCACACATTGTATAATTCAATTAATTTGCA







CATCCAACTCCTCAAATCTGGTCTTTTTAAAATGACCAACTGCTT







AAACTTCATCTGTATTGAAAAATACAACATTAGATCCAGCCATAT







TTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GPM6B. In some embodiments, the sequences from gene GPM6B comprise or consist of:











(SEQ ID NO: 204)



GGCAATTGCTATTCTCTGTGAGCCAACCTGTTACGAAAAATAAAG







GTAACTTTTGAGAAATTAATATGAAGTTATAATTCAGTGGTATAT







GGAATACATCCAGAATCTATCCCATGTTAAGTAGCATGTTCTGCA







TTTTCAGAACGGCTATTTAGAAAGAGCACCTCTTGAGAGGCTGCC







ACTGATTAGCTCTGTGACCTTGGATGAGTGAGATCACCTCTTGGG







GTCTCCTGTCCTTGTTAGTTAAGAGTGACCCGAAAGGCCCTGACA







TTGCTTCGGGCACTCCAATTCTAGATCCTCTAGCTCTGGGGAATG







TGGCTGGCTCTTACTTCCCCTGCTCCCGTGCTGGCTTTTCCTTCT







TGCTACCAATGCCGGGGCGAGGGTCCCCACAATGTGTTCCACAGG







TCAGTATTGGGCCTGAAAATGCACAATCCTTCCACTTAGGGGACA







CACATCTACCTGTTTCAGTGATGATTGTCCTTCCTCTTGTTTTGG







CCTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MIDI IP1. In some embodiments, the sequences from gene MIDI IP1 comprise or consist of:











(SEQ ID NO: 205)



CTCAGACAAGGCTTTTACCGGAAATTTAGCATATAGGATAGAAAG







CAGAGACCAAAGACCTCTTGCTTTGAGGCTGTTGGCGTGCTACAA







GGACTCGAAAGTTATTTGTTCAACGGATGACTTCTGTTTTCTGGA







GGCAAACTGAATTCCGGGCAGGTGGCTCAGCACCTCCAGCCTGTT







CCAGATCGGGACAGGGAAGGTGCCTGGGGCAATCAGTGAGGTTTC







CAACCTTCCTTAGGCCTGGGCCAGCCCGCTCCCCTCCCATGTGAC







CCCAGGCAACCATTTTAATTTTTTTACAGCCAAGCGTAGTGGAGA







ACTGGCTGCGCGCCGGTTGCCAGGGACGCGGCAGCCAGCTTGGCG







GGGCTGGGGTGAAAGAGGACGCTGCGTGGGGGAGGGCTCCGCGGC







TTCTGACTGGCTGGAGCATCAGGATTCATTTGCATAATATTTTCT







GACGGCTTCTGATTGGCTGAAGCCAGAGGGGCGGTGGTGGGGTGA







TTCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MIDI IP1. In some embodiments, the sequences from gene MIDI IP1 comprise or consist of:











(SEQ ID NO: 206)



TGAGTTGTTGCGGGGTCTGGGGGAGCTGGGAGGCCCGGTTTGGGA







AGTTTTGCAGGCAGGTGCATGAGCCATCTTTCTCTGAAAGTGTTT







AAGAAATGTTCAAGAACAGCGGCCAGAGTCAAGAAACTGGGGGAT







GAAGGGTTATCTCGAGACTAGACGGAAGGAAACGTATCAGGGGAG







GGGTGGAGGCCAGAAGGAAGGAGTTAGGTAGGGGCCCTCTCCCAA







GCTGGCGTTTTTGCTGGGTGGAATCCTTTTGCTAGATCCCTTGGA







TTTCCGTTTTCCGTGTGTTATCACACTGTTGGAACTGGGAAGAAG







AGAAAAAGAGGCAAGATTTTGGCATTTAGTAAGTTAAGTCTGAAT







ACCGAGTTTTCATCCGGGTGCATACGTACTTAAAATACCAAATGG







GTCCAGGAAGGGGGACAAAGTACGCTTTAATTTATGAGAAGATCA







GCTACTAGAAACTGTATATCTGTTTTTCTCGAGTTTTCTTCTTGC







AGTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SYN1. In some embodiments, the sequences from gene SYN1 comprise or consist of:











(SEQ ID NO: 207)



GTGAGTTTGGGGAAAATAGAGTGGGCAGGAGGGAAGAGTCATAAC







TACTGCAATAATATTAAATATTGGTATATTATCAGCAGTGCGATA







TCAAGAGTGTGGCTGCTGTGTGCACGTGGAGTCATGAAGTTGCTT







TGTGATAGGGTCTACCGTGAATTTCCTTATGCTTACAAGGAAAAC







TAAATCATGATCCATTCTAATATGTATCAGATCCTGTTACTCATG







TTGTCTATTTTTGCCAGTATTAAAAACCTATTTCTAATGTGAATT







GACAGAATTAATGTAATGAATCAGAATATATTACATATAGAAATT







AAGTCATTATTACTAACATTATCAAATATTCCTTCTGATAGAAAT







TTTACCTATATTCAAATTAGGATTGCCAATAACATTTATCAATAA







TAATGTTATAAATTAAATTGTTGGCAAGGCACGGTGGCTCATGCC







TGTAATCCCAGCACTTTGAGAGGCTGAGGTGAGCAGATCATGAGG







TCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SYN1. In some embodiments, the sequences from gene SYN1 comprise or consist of:











(SEQ ID NO: 208)



GATCACCAGGTGGGCAGAGGGTAGAAAGGGTGCACTAGGCAGAGG







GAACAGCTCACACATGTGTGCAGAGGTGAGAAACCACTTTGCAAT







TTCTGGGAGCTATGGAGATTTAAAAACTTGGTCTTTGTTTCGAGA







GTGATTGGGAGCTTCTGGGGGAACTCTCAACACTCCTACCCCTGC







CACACTAGGTCTCTGAAGCCGGATTTTGTGCTGATCCGCCAGCAC







GCCTTCAGCATGGCACGCAACGGAGACTACCGCAGTTTGGTCATT







GGGCTGCAGTATGCTGGAATCCCCAGTGTTAACTCCTTGCATTCT







GTCTACAACTTCTGTGACAAGCCCTGGGTGGTAAGTAACAGGAAG







GGTGCCACTGGCAGAGGGAACAGCTCATGCATGTATGTGGGAGCT







TGAAACCAGTCTGCGATTTGGGGAAGTGAGGGTGTTCAGAAACTT







ACAGATTCGTGGTGAAGGACTCACTGACCCAGCTCTCCTGGTTCT







CATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PQBP1. In some embodiments, the sequences from gene PQBP1 comprise or consist of:











(SEQ ID NO: 209)



GCGTCTGGCCGCGCAGTCAGGCCACGCCCCCAGCGTAGACGCACA







CCGGCGTCGGAGCTTTCCGCCTATTACCGGGCAGCGATTGGGTCG







CTATACCGCATGTCACGCCAAGGACGCACTCTCATTGGCCAATCG







AGTTTCTGCCATTTGTTCATTGCCTCCTGAGCGTAGTCCAGTTAC







TTTCAGGCTCGGGGAGTGAAGGCCTCGTTGAGAGAAGGTCTCATT







CGGTGTTTTGGGAAGAGAGTCGTGTGGGCCCAGGTATCGTAGCGG







CGACACGAGAGAGACGGGCGGTGTGACAGCCTTCCACTACCTGCA







CGAGTGTATTGGTAACGTTGGGGTGGGTGCACTCTTTTGGAGCTG







GAGGAAGTGCTGCCCTCTGCTCCCCCATCCCAGCGCTTTGGTTTC







TCCCACCCGGCAGTCATGGGGACGCTGGATGAGCCCATTTCTGAA







GTGCGGAGGGGCCGGGCTTCTTAGGCTGTGGGCGGAGCCTGGGCC







TGGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PQBP1. In some embodiments, the sequences from gene PQBP1 comprise or consist of:











(SEQ ID NO: 210)



TGCGTATGTTCTGTCCACCCAGCTACTGGTTCATTTGAGTAGATC







GGAGTCAGATGAGTACATGTTTACGGGAGGCGCTTGGTTACGGGA







TCCAGATAGGAATCTTTATCTGAGCTGCTTGTCAGTTTGTTCGTC







TGTCCCTAGGTCTGTCTGCTATCAGCTATGCCGCTGCCCGTTGCG







CTGCAGACCCGCTTGGCCAAGAGAGGCATCCTCAAACATCTGGAG







CCTGGTGAGACAGCTAAAAGCAGATGGTCCTAACACGTGCCAGCC







TCGACAGGCACTTTTGTTGTTGATACTGACGCTTGATACCTAGCA







TGGGCCAACTGTGCACCAAGCAATGGGGTGGCAGGCATTTTTCGT







TGTTAACAATGTGGATTCCTGACATCCATCCCTACCGCGTGTCAA







GTGGAGGGGGTTGGGGGAATAGATATTTCATTCTTTTTTTTTTTT







TTTTTTTTTTTTTTTTTTTTTGAGGCAGAGTCTTATTGTGCACCC







AGGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CD99L2. In some embodiments, the sequences from gene CD99L2 comprise or consist of:











(SEQ ID NO: 211)



GTAACGCTGCTTTGCCTTCCTCACTTTCCTTTTGCTTCAGCCTTG







ATGCTTCCTCATTCGCCCCCTGCTCTGGCGCTTCCTTGCCGCTCT







TCTCCCTCCTGCTTCCCTGGCACTCACCGACGTGGCTGTAACCAG







GACCATCTTTAGCTTACCCTGATGCGTGTGAGAGGATGCCACCTG







CCAGACACTCTGTCCCCAGGCTGGATGTGGCAGGGGTGGGGGGAG







AGTTGAGGTGCCATGGTGAGGACTCAGGTGCCCACTGGTGGTCAT







GCCTGGGGAGCACATAGAGGGCCACCCACAGGAAGGAGACGATCC







AGCGCCCACTGGGTTGGCAGCTAGCAGTGATCACGAGGCTCAGGG







TGTGTCTCCTGGCCCCCACCTGTCAGCTGGGAAGGTGCACCTGGG







GCAGCTTCCACTGCACTCATCACCTGCCCCGCTCTGCGTGGGGCC







GGCCACACCTGCCAAAGAGCAACGCCCGCATTTGCCACAGGCCCA







TTTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CD99L2. In some embodiments, the sequences from gene CD99L2 comprise or consist of:











(SEQ ID NO: 212)



CATGGGGCGGGATTGGGCCGGTTGGTTTCTGAGTGTGCGACTCCC







AGCTCGCCTTGCCCCCTCCGTGATCAGGAGTCTCTGGACCGTCCA







TTCCGTGGCGGGGCTGCGTGTACATCATGTCCCTGGCACCTGGCC







CGGGCCAGAATCAGGAGGAGTCCCGCAGACGTTTGTGGGGTGGCT







GAAAACACTGAGAAGCTGGGGGTTGGGCCTCCTGTCCCCACTCTT







TCCCCTGAGTCTCAGCCACTGCCACATGGAGATGGCGGTAGAGGG







GACCGGGGTGCAGAACACCAAGCTCTGGGGAGACAGCAGGAGGTG







GCTGCGAGGAAGGCCTGCCCCCACCACTGTGGCCCTCAGGTGCTC







TCGGCCTCTGCTGCTGCCAAAAGGCCCATGCTGGGGACCCTTGGC







TTCCCTTGCCTTTGCCCTCAGTGTGGAAAGCCCGCATGTTTGCTG







TCAGGAGCGCTCTTACTCCAGGGATTCTAAGCTTTGTGTCTTGCC







TTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene L1CAM. In some embodiments, the sequences from gene L1CAM comprise or consist of:











(SEQ ID NO: 213)



GTAAGGCAGGGGCGGTGGGGAGGGGCCAACAGCAAGGTCTCCCTAT







AGACAATGTGCGCCTGGTGTGCCATTGTCCCCTGGCTGCCGGGGC







TCTGACAGGACTCTCACTTCCCAGCCCCCAGGCCCGGCAGCTCCT







CATTTCAGGGAGAGGCCAACACGCCCCAGCCCCTCCTCTCCACAC







AGATACCTTCACTGCTCCCCTCTCCCCAAGGGAGGCCCCAGACAG







CTTCCCAGACAGGAACCAGGCCCCATAATCCCCTGGCCGAGCGCA







CATTTTCTCCCTCACAGTCCCCAGGATCTGGGGAATGCCCAAAGA







TGACAGCTCCAGACCTGCCTGCCAGGGTGGCCAGAGCTGCTCTTT







CTGAGCGCATTCTAAACAAATGGAAGGCAGGCGGGCGTCTGCCTC







ACCCCCAGCCAGGCTCCTGCAGGGGCTCGGCAGTGCTCTCACTCG







CACCTGCCCTGTGCCTCTGCAGTGACAACGAGGAGAAGGCCTTTG







GCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene L1CAM. In some embodiments, the sequences from gene L1CAM comprise or consist of:











(SEQ ID NO: 214)



GCCTCTGAGCCCCACGTGACGGGGGTGGAGCCGAGGGCAGAGAGG







AGCACCTCGCCCCTTCCACCCTTCTGCAAGGCCTCCTGGATTCCC







CGAGCTCCCCACAAGATGACAGGCGCATGGTGGGGGCAGGCAGGG







GGATCATCCTCCCAGGCCGGGGTTGGGGCAACAGGCAAGTGGTGG







CTGGACCGCAGGGGGTGAGCGGTTTCTTTCAAGGCCTCTTGCCTC







TGGGCGCTGTTGAGACAGAGTGCTGGGGGTGCGGGGCGTAGAGGG







GCCTGGAGCCCCGGGTCAGGCTGGGGCGGGAGAAGAAGCTGTCCC







TCTCTGTGTCTCCAGTGAAGGATAAGGAGGACACCCAGGTGGACT







CTGAGGCCCGACCGATGAAAGATGAGACCTTCGGCGAGTACAGGT







GAGCCGGGGCAGGAGTGGGTGCTGGCACTCAGCTCCACCCTGGCC







TTCACATCTCACCCCCTCTCTCTCTTCTCTGTGCTGCCGGGTGAC







TTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CCDC3. In some embodiments, the sequences from gene CCDC3 comprise or consist of:











(SEQ ID NO: 215)



GTAAGTAAGCCATTTGATTTCTGCTGCCTTTGTTTTCCCATAAAA







GGGCGTTTAAAAAAATATAATTCTCCAAGAAATCTCTAGTTGTTT







TGAAATAAAAAGTTACTTTTCGCTCTTCATCTCATTTATTCCAGC







TATTCTCTGCTTTGCCCTCCTTGGTTTGTGGAGCCTCATACAAAT







TCATTAGTACTTTTCCAGCGTCACAGGTTGGAACTGAGGGTTCCA







AAGAGCAAAATGCATTTTGGCTGAAGCAGAGCCAGGAATCCATGT







TGTTTGCCATTGGCGCCATCCAATTCACTTGTTCTGAATAAGGGA







ATAAAGTTAATATTTATTCCCTTCATGATTCTTGTATTTCAAGAA







AAAAAAGTGCTCTACAGGCTTTTTCCCCCCAGCAGGTACTGATTG







ATTTGTTTGAATTTTGAGCTGCAATCAAGGATGGAGAATCTAACT







GATCTCCTTATTCACCAAATATTTATGATTTGTGCATAGAATATT







GACT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CCDC3. In some embodiments, the sequences from gene CCDC3 comprise or consist of:











(SEQ ID NO: 216)



TGATGACTATTACCAGTTTTCTCTAAGCCTAGGCCCAGCCCTGGC







CCATGGCCTCAGCCCTTGGATTGATATGGCTTATTTAGGTATTTA







AAGGTCGACACCTGCCTCCTGCTGTTTAATTCCCTGCAGTCTGAC







AGATTCACCAATTACCAAGGAGAAAGCCTGCCTTCCTTGTGCTGT







GTGTAAATGAGGCTTCCATCAGCACAGACACACACACAGGCTGCA







AATGGACCAGCCCTGCCCCCAATTCCACACCCACGGCCACAGCAA







AGAATGCTGTGTTCCCCTTCCCCAGCCCTCACACCCAGGTTAATA







CCTGGGCTCACAGCCTCAGCCCCACACCCAGGTTAATACCTGGGC







TAATTTCATTTTTTCACTTTTTGGGTTTTCAGATGTACACCCCAG







CGGAATCAAAATGACAGATTTATGTCACATTGTGGGTTTGGATCA







GAAAGCACCCTTGAGCAACACTTTGATTCTGTGTTTCTTCCCGTC







CTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene FAM107B. In some embodiments, the sequences from gene FAM107B comprise or consist of:











(SEQ ID NO: 217)



GTAAGAATAAACACTGGGACTTTTCTATGGCTGTTGAATAATGTA







GTCCTTCTCTAGAAAATGTTGTGTGAACTTAGACTTGTTAACACA







ATAGCAGACTTGTATGAGACTAAATAAAAATTATATGTTGTTATA







GTGAGTGGTATTGAATAGTGTGTCTTTGCCAGGAAGAGAGCTTTG







TAATAGGCTGTGTCTTGTGTAGTTGTGAACTATTTTTTTTTCCTA







AAATTTCTTCAGATGCCAACCATGTCTGTCAAACTCTGGATGAGT







CGCCTTTTACTTAGCTTTTTAAGAACTCTTTTTGGATTCACTAAC







TTTTATCTGCATTATTTTATCCATGCTCTATTAAGTGTCTAGGAT







CCTGATAATTATGCATTTTCTCTGAAGATATCTATTTCCAGTAAA







ATTTGACTATCTAGTGAAGATTGTAGGCCTGATAATATATTTTGA







AGTACTTGTTATGATCTGAATGTGTGTGTCACCCCCAAAAATGCA







TATG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene FAM107B. In some embodiments, the sequences from gene FAM107B comprise or consist of:











(SEQ ID NO: 218)



CGAGAACTTGTCCTCCTCCAATTGGAGGTTGACTCACGCATGCTT







CCTGCAGTCAGGTTGTGTGAGTGTTGCAACCTGGTACCCACCCAG







GGCTGGAGAGTCTGCGTGCATATTTGCTTACAAGTAGAGGAGCTG







ATTTTTGTACAGACAAGTACATTTCACCAGTGCTGCCAGCACGTT







CTCTTGGTCTGGAAGGTCAGTAACTGGTACCAGAGTTGGTACGGT







TTGTTTTTCATTGTTTGCTCTGGCTTCTGTGCCGGATGAGGTCAT







TCAGTTGCATACAGTTCCTGCTTTCAGAAGTTGCTAGAGTGTTTC







TCTTTGAACTTGAACTGTGGCTCTTTTGATATCCAGAAAGAGAGT







TCTTTGTGGGGATGGTTAATTCTTGTTTTGGGTGCTTTTCTTCAC







CGCTCTAAGGAACTACCACAGTCAGAAGGTGCATTCTTACTGCGA







TCTTTTTATTTGCTACCTTGGGCTTAAGGAGCCTGCCTATATCTA







CTGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NEBL. In some embodiments, the sequences from gene NEBL comprise or consist of:











(SEQ ID NO: 219)



GTAAGCTGACGGCGTTTGTCGGCCGGTGTGTGTGGTCGAAAAGAT







CATGCTTGCTCTAGCTTCAGAATTACCCTCTTGAGTCATCTGACT







CCTCATCACATGTTGGTTGTGGTACCCAATTTAAAATGGACCGCA







GGTAAAGTATTATTTTTGTACGATGTGGAATGGGATTTCTGGCAT







TCACCTGGTATCATCTCAAGTCTTTTCTATTATTGGAAGCTCTGT







CTTTAATACCTTGCTAATTTTAAGAATGTTCCTCCTTGTATCACA







CACCAGGGCCTGTTGTGAGGTGGGGGGAGGGGGGAGGGATAGCAT







TAGGAGATACACCTAATGTAAATGACGAGTTAAGGGTGCAGCACA







CCAACGTGGCACATGTATACGTATGTAACAAACCTGCATGTTGTG







CACATGTACCCTAGAACTTCAAGTATAATAATAATAAAAAAAAGA







ATGTTCCTCCTTGTTAAGCAAAGAAGCAGCAGAGATATGAGTGAT







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NEBL. In some embodiments, the sequences from gene NEBL comprise or consist of:











(SEQ ID NO: 220)



AAAAAAAGTATATTTTGAAGTATAAGTTTTTGTAATTATATTAAA







AGTGGTATATATTGTTACCTATAAAATTTCAAACTGATTTTAAAT







AATGCCATTTTCATGAATGATTGCCTGGAAATAGATATCACTTTA







TTGCATACTGAAGTTGAAGAGATAGGAAATTTAATTTATATTTAA







ATTAGTATATTTTAGGAAGTAACCAGGATTTGAGAATATGTATTT







TTACAAGCTCTCATTAATAATATATATATAGTTTTATATATAATT







TAAAATAGATATGAGCTATAATGATTAAAAACTTTGTGAAAATAT







CAGCACTTGATTTCTGATGCTAAGAAACTGAAGTTCCAGACTTTA







GTTTTGGAGTATTTGGAAAAGATACATACATGTAGACAATCTGCA







CGCAGCCAGTGCAGTGTACACCTGTCATTTAATAAAAATGTTGTG







AAAGAGGTAACTATCCGCAAAATTCAGTATGATGATTTTTCCTTT







TTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene HNRNPF. In some embodiments, the sequences from gene HNRNPF comprise or consist of:











(SEQ ID NO: 221)



GTGAGGGGCGGCCGAAGTCTCCGAGGTCGAAGGCCGCACACCCGG







GACCTAGCGGGGACCGGGTCCCCCTGGGGGTGGGGACGGGGGGTG







GGATTTGAGATTAGGGAGACTATCCGAAGTCCAGGGCCCCGTACC







CGGACTACCTGGGGACTGGGCCTATTGGGGTGGGGATCGTTGGGC







TGGGGCTGGGATTTGGGATCAGGGAAGAGCGATCTGAGTTTAAGC







GCCGCAAACCTGGGACCGAGCGAGGACTGCACATAATGAGGGGCC







GTGGCGCTGAGATTTGGGTGACGGGATTCGAGGTCGAGGGGCCCG







TACGTGGGACCGGCAAGAACTGGACCCACTGGGATCGGGAGACGC







AGCGTCGGAGGTCGAGAGCCGCACGCCCAGACAGTGGTCCCACGC







GGTGAGCACCGCGGGCTGAGATTTTGGAGTCAGGAAAGCGCGGGG







AAGGCACGGACGGCCGGGTGCGTTAGTCCGGCCCCCTGGCGGGGA







GCTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene HNRNPF. In some embodiments, the sequences from gene HNRNPF comprise or consist of:











(SEQ ID NO: 222)



CCCTATCCCCTCCCCTCCCCTCCCTTCTCCTCTCCAGAAACGTCC







TCGCTTGTCCGCCTCCACCGCCTCCCCCGCCAGCCGTGTCGGCTC







TGCTCCGCGCCCAGCCGGCCAACCGGCGGCTCAGCTCTGGCGCGT







CACAATGCGCCGTCGCGGCCCCGCCCCCGGACGCCGGACGCAGAA







ACTCCGCCCTGCCGCGGTCCTTGCGTCCTTCCGGCTCCGTCGTGG







AAGCAGGACTGCGCCGCGTCTTCCTCAAGGTGGGGTTCGGGGGGC







CACCGAATCGCTGTCACGCCGGCAGGTAGCAGGGGGTGGGGACGC







GGGCTCCTTGGGCTTCTGTTTCCTGGTGTCCAGGGCAAAGGGCTG







AGCCGGGCAGTAGGGCCTAGTCGGGGCCTGGCTCGCCGGGAGCAG







GAGGGCTTCCGGCAAAGCCGCGTGCGCTCGACGGGCAGGCGCTGA







GGATTGGGGCCGGCAGCGCGCCTGACCCCGCCCCTCGTAGGCTCC







GCCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene FAM213A. In some embodiments, the sequences from gene FAM213A comprise or consist of:











(SEQ ID NO: 223)



AGGGAGTGTGGGTTGAGGGAAGCCACCGATGTGGCAGAAAAGGAA







GGGAAAGCAAAAGGAGGTAAAGAATAGACAGCAGATGAGGGGCCT







CAGAGGTGCCAGCTGACTTTCCAGGCCTGGGGTGAGGAATGGGGC







AAGGGAGCAGGCTGCACGAGGAGCCACAGAGAAAAGGACATCTTC







TTTTATTTGCGCTAGTATGACATGTGGGCTGTGTTACGAGCTTTG







CTTCTTTAGTCTGCACCGGATAGCAGAGGAGTCTGGTGGCACTTG







GGAAATGTTATAATCTGCATGACTGCAAATGACTCAAAGCTTTGC







TCAACTTTTTGAAGGGCTAGGGGGCCTTGGCAGCTTTCCATTTAG







ATGTTTTCTGGAGACTGACTGTTGGCAGCTGAAGGAGCCCCAGGG







TTTCTGGGTGGCCTGGTCCTGCCTATGGAGTGACTGAGCCTAGGG







ACCTAGTCCCGCCTCCAGCCCTCCCCTTGCCGCCTGCCCAGGCCC







GTCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene FAM213A. In some embodiments, the sequences from gene FAM213A comprise or consist of:











(SEQ ID NO: 224)



TGAGTGCCTTGTTCCCAAGTTTGAAGCGTCTGAGGTTGGGGGTAA







GAGACCGTTTGAGGAAGGGAGTTGCCTTGTATAGGAAAGAGACAG







GGTCTCGAAGCTGGAAACTGAATTTGGGGCGGTGGGGGCAGTGTT







GAGGAGCCTAGAATGTTCTGACTCCAAAGTGACCTGTTTTTGTTA







GACTCATAGGATGTCACTGGGGATCATTAGGAACTTCCTCATGGA







GTTGAGAAAAATGGGGCCTGGGGAGGAATTGATTCAGCTTAGCCT







AGGGAGCCCGACTGGGTCTCGTCCTCTTGCACCCATGTTCAGACC







AGAGCTGCCACTCTGAGCTGCCACAGCTGTTGTTTGTTTGTGGCT







TTATCCTACTTCACCTTCTGTGGGAGAGGGTGGATCAGATAAGCC







CAGCAGTGGGGAACTGAATGCTATAGCTGTGTCAGGTGGGGGCTG







GCTCTTCTGTAAACAACCCTACTATTCTGTTTCAGAGAAACTGAC







CCTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NDUFB8. In some embodiments, the sequences from gene NDUFB8 comprise or consist of:











(SEQ ID NO: 225)



GTGAAGATGGCGGTGGCCAGGGCCGGGGTCTTGGGAGTCCAGTGG







CTGCAAAGGGCATCCCGGAACGTGATGCCGCTGGGCGCACGGACA







GGTCCGCGAGAACGGGCGAGACCTCAGTGTGGGAAGGGGGTACCT







GAAATTTGGTGTATCGGGAGGGGGGATCGTAGATGCCGCGGCGGC







AGCCTCCGGGTAGGGAGTGGAGGTGGCGTTGATATATCCAGAAAA







TGGGGTGGAGGATCGCACTGATCCTGCGAAGTCCCGGGCTCGGAC







CTCTGCAGCCCCTGCGACTTGAAGCCTTCGGAGCGGCTGGAGAGC







GGGCTCGCTTCTGACCTGTTCTCTTCTGCGTTCTAGCCTCCCACA







TGACCAAGGACATGTTCCCGGGGCCCTATCCTAGGACCCCAGAAG







AACGGGCCGCCGCCGCCAAGAAGTATAATATGCGTGTGGAAGACT







ACGAACCTTACCCGGATGATGGCATGGGGTGAGACAGAGAGGGTA







TGCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NDUFB8. In some embodiments, the sequences from gene NDUFB8 comprise or consist of:











(SEQ ID NO: 226)



TGGGTTGAGGTGACTGAGACATGCAGATGGAAACACGGACTGAAA







CTAAGGAGCCAATTCAGGGCCGAATTGTGTGTTGCACTGGCGTCT







AAGTGAAGATCAACCCATTTGGAGAACAGAACAACAAGCAAGACC







AACAGGTGAATTTGAAGAATGATCATGTTTGGGGATGGAAGGAAG







TGCCCGTGAAGGAGAGGAAAGCAAGCAGCCAGAGAAGAGAGTAAG







ACAGGCTCATTTCACTAGTACAGCTCCTGGGGGAGGTGGTTAAGC







TGTCTACCTGAGTTGCTCAGGTTCCGAGTTCCCATCATCCCTGGA







TGGGTCTAAGTCAGCTCAGGGAGAAAAAGGACACAACATTCTGAA







ATGTCAGAGACCTGCCTGGTTTTCCCTTCTTCCCGCTTATCCTTC







CCAGCCTCTATCCAACCCTTGGCTTCCCAACTGCGTATTTAAACC







CCGCCCCTGTTGAACCCTTCGGCTTTTCTTCACTCCCGGCTTCCT







CCGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PSD. In some embodiments, the sequences from gene PSD comprise or consist of:











(SEQ ID NO: 227)



GTGAGTGCCCTCCTCCTGCCCATCCCGGACCCCTTCCCCCACCTC







CCATCCTGCTCCCCTCCCTGTCCCTGGATTTTTTCATTCCTTCTC







AGGCCACACTTGAGCCAGGACTGGCATCCTGGGACAGAAACCCTG







GGCCCAGGTCCCAAAGGGCTGTGCTTTCTCCCCAGGAGCCTGGAG







CAGATGCAGTCCTGGATCACTCGCATCAATGTAGTAGCCGCTATG







TTCTCTGCGCCCCCCTTCCCAGCTGCTGTTAGCTCCCAAAAGAAG







TTCAGCCGCCCTCTCCTGCCCAGCGCTGCCACCCGCCTCTCCCAG







GTAGCCCAAGCTCTGTCCACCTAGTGCCAGTGGGATGGAGGGTGT







TAGGCTCAGGCCCACTGCACTCAAGGCTGCTGCGTGTGATCGTGG







AGATGTCCCCTGCACCAGGATGCTGGCCACAGGGGTGAGCAGGGA







CTGAAATCCAGCCCCCACTGGGTCCCGCTTGCCGGCTGGGAACCC







AAAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PSD. In some embodiments, the sequences from gene PSD comprise or consist of:











(SEQ ID NO: 228)



GAGGTGATCACACCTGCAGAGACGAGGAGGAGCTGAGACGCTCTC







TGTCTGAGTTGGCCGACCCCAACCCCAAGGTCATCAAGCGGATCA







GCGGGGGCAGTGGCAGTGGCTCCAGCCCTTTCCTGGACCTGACTC







CCGAGCCTGGGGCTGCCGTCTACAAGCACGGGGCCCTGGTGCGAA







AGGTGCACGCAGACCCTGACTGCAGGAAGAGTATGTGGCCGAGTT







GCGGGGGGCGGGCGTCTAGGGGATGGATGGGAGTGGGCCTGTTCC







AGGGCCCCCTGACCTGTCTTCTCTCCCCGCAGCACCTCGGGGCAA







GCGGGGCTGGAAGAGCTTCCACGGGATCCTCAAGGGCATGATCCT







CTACCTGCAGAAGGTGCGGGGTCAGGCCAGGGGGCTGGGGCTGGG







ACTGAGTTGGCTGGTGGGTGTGGGGTGATGGGTGGGAATAGGAGG







GGCTGGGCCAGGCTGCATTTGGGGCCAGGACAAGATGGACACCCC







TGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GSTO1. In some embodiments, the sequences from gene GSTO1 comprise or consist of:











(SEQ ID NO: 229)



AGAGGCCCTCCCAGTAGTCACGTTAAGCTAGTACTGACAGAAAAA







GGAAAAGCCTGTCCAGGTGGCTGGGCAACCCTGCAGCGAGGCATA







GATGAGCTTGTAAATCAAAGGAAATGACCAAGGTGAGTCTCAATC







CTTTTAGAGGTTTATTTTGCCAAGGTTGAGGACACATCTGAGAAA







AAGGAACACAAAATCACAGGAACATCTGTGATCTGTGCTCTTTCC







AAAGAGGGCTTGAGGACTTCAATATTTAAAGGGAAAGAGCAGGCA







GTAGGGGAAAGTGGAAAGTAAAAAGGGAAGGTAGATAAAAGGGGA







GAGTAGTTGCATTCTTTGGGGCCTTTGATCAGCATTCACTGAATG







CGCATGTTACATTCGTGAAAGGAGGTCGTAGAGGAATAGTCAGTT







ATGCATTCGTCTAGTGCTTGGTGAATCTGCATTTTTACAAGATTA







AATATAGGGTAGAGGAAGCGGTCAAATTGCATTTTTCTCCAGCGA







GAGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GSTO1. In some embodiments, the sequences from gene GSTO1 comprise or consist of:











(SEQ ID NO: 230)



TGAGTGAGCCATGAGGTCGGGCCACAGGCTCTGAAGTCGTGAAGC







CTTGGGACGGAGCGGGTGGTGCTTCGAGGTCAGTGTCACGGGAGG







GAGGTCAGGGTCAGGGTCAGACGTGGAGCCCCGTGGAGTGCGGAG







TGGTGACCCCTTCGTTCGGGGGCGGAGGGATGACTGAGCATTTAT







AACTTTTGTGTATCTCCCCGTGGGTGCAGCCCTTGGCCAGCGAGA







TGCTTTGACACAGCCCCTTAAGATGTTTTAAGGGCTATCCCATAA







AGCCGGGAAGGCACAACGGGTGGAGCTGGCGGCCGCCGGGGGCAG







GCACTTTTGAGCTAAGGAGGAAGCGGGGGAGGGAAGGAGGGCGGG







AAGGACGCGCCACCTACTTCCTGAATCCCCTGCAAACCCCAGAGG







AGCTCGGCCTGCGCTGCGCCACGATGTCCGGGGAGTCAGCCAGGA







GCTTGGGGAAGGGTGAGGCCTGCCCGCCGCGAAGAGGGGGTGATC







TCGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TACC2. In some embodiments, the sequences from gene TACC2 comprise or consist of:











(SEQ ID NO: 231)



CGTATGCCTTCCTGCAGGATTTCTCTGTCTTCCTGTCCCACTCTT







GACCCTTGCTTTAGCCACACTGGCCTCCTCGCTGTTTCTGTAACG







CACCAGGTGCTCCTGCCGCAGAGCCTTTACTCGCACTATTCCTTC







TACCCAGGATGCTTCCTCCCCAGGAGTCCTCCTGTTTCATGCACT







TACCTTGATTCTGGCCTTTGCTTAGATGTCTCCTCAGAAGATCTT







GCCTGACTACCTTATTTTAAAAGTGAAACTCTCCTATCCCGGCTT







TATTTTTCTCCTTAGCATGTATCACCCTCTGAAATTCTGTACATT







GTTTGTTTGTTTTTGAGACAGAGTCTCACTCACTCTGTCACCCAG







GCTAGAGTACAGTGGCGCAATCTCGGCTCTCTGCAACCTCTGGCT







CCCAGGCTCAAGCGATTCTCCTGCCTCAGGCTCCGGAATAGCTGG







GATTACAGGCGCTCACCACCATGCCTGGCTAATTTTTGTGTTTTT







TTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TACC2. In some embodiments, the sequences from gene TACC2 comprise or consist of:











(SEQ ID NO: 232)



TGTGAGACACCATGCCCGGCCCTGGTGGTTTATTCGCTATCTTCC







CCTGAAGAATGTGACTTCCAGGAAGGGAAAGGTTTTGTCTATTTC







TGTTCCTTGTTGAATCTCTAGTACCTAGAACCATGTCTGGTCCTG







ATTAGGCACTTGGTGAATGTTTTTGAATGAATACTCTGAGCTGTT







CTTTAATCGGTCCTCATGATCCTCATTGTACAGATGAGGGAACTC







TGGGCGAACCTGGCCTGGGTCTTGAGCTAATTACGGGTAGGTCAG







GTTCTGTACCCAAGTAGTACACACAGTGATGGGCGGGGTGGCCTG







GGGCCGTGGTTTGTCAACCCCTACGCTGGAGGGTGATGTTTTGGT







ACAAGAGGAGAGGTGCCCCAATGCGTCCTGTGTCTGTAATTGATG







GCGTTGTCTGTGTTTCCCCAGGATGTGGTTCTGAGACAGTCCCTG







TCCCTGATGGCCCACGGAGCGACTCGGTGGAAGGAAGTCCCTTCC







GTCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TM7SF2. In some embodiments, the sequences from gene TM7SF2 comprise or consist of:











(SEQ ID NO: 233)



TGCCTAGGGGACGGGCCCTCGCGCTGGAGTTAAGCGGCCGGGGGT







GGAGCTCCAGGCCTAGCGGGGAGGTCCACGGAGATTGGCCCCCAC







CCCAGGCTCCTTTGTGTGCCTCTGTTACGCCCAGGACAGACGCCG







GGGGCTCTCCCTATGCCCCTCGTCCCCACAGCCTTACCCCATTTA







CTGCTACTCCGTGCACCACAGCAGTGGCTGGGGTGATCCTCTGAA







GGCCAGGGTGGCAGCCTAATGCTTGGGGCAGGGTCTGTGGCCCCC







CTGAGTTTTGGGCTCTGCGTGTGTTTGCGGGGTGGGGATGTGGAG







GCGCGCAGCCCCAGGGCTCACTGTGCGCTGTGGTTCAGGCTTCCA







GGCCCTGGTGCTGACAGCCCTGTTGGTGGGGCTGGGGATGTCAGC







GGGGCTGCCTCTGGGGGCGCTCCCGGAAATGCTCCTGCCCTTGGC







GTTTGTCGCCACCCTCACCGCTTTCATCTTCAGCCTCTTTCTCTA







CATG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene APBB1. In some embodiments, the sequences from gene APBB1 comprise or consist of:











(SEQ ID NO: 234)



GAATAGGGCTTGGGATTCGAGGGTGGGAGTTCATCCAGCATCAGA







GTCCTCCTCTCTAACTCTGTTATTTGCCTCTCTTGTGCTGCTGGA







CCCAGAGAGAGGTACTATGCCTATTTTCCCCCTCCTACACCTGGA







CCAGATGCCTGGAAGCACCCAAGCTCCCCTCTTCTGCCCTGCCCC







TTCCCACCCGCTATGCTCCACCATTACCCTCCACCTCAGCATGCT







TGCCTGTCTACCCCTCCCCCCCAAGAAGGCAGCACCTATGGGGGA







CCAGCTGTGGGCAGAAGCCCTGGAGGAGCTGTGATGGGCAGGGAG







GACGTGGGTGGGGTGGGGACCAGGCTCAGCACTGGATGGTACTGG







GAGACACTGAGGTGCCCCTTCCCCAACAGGGACTTTGCCTACGTA







GCTCGTGATAAGCTGACCCAGATGCTCAAGTGCCACGTGTTTCGC







TGTGAGGCACCTGCCAAGAACATCGCCACCAGCCTGCATGAGATC







TGCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene APBB1. In some embodiments, the sequences from gene APBB1 comprise or consist of:











(SEQ ID NO: 235)



GGGGAAGTAAGGACCTGAGCCGGGGGTAGGGGGTGGTACTGTGAG







AGTGTGAAGGGAATTATGAGAGATGGGAGGTCTGACCTGCCCACA







AGCAGGCTGGGGAAGGGGGATATTGGCCCATCCTAAGTGGCAGCC







CTGTGGGATGGATGAGCGACCCCAGGCCAGGACCTTGGGAGATAG







TCGCCTAACAACGGTGGAGTCAATGTGAGAGATGACACGTGACCT







CCACTAGAGTGAGGCCTGCAGGGCGGGCGGTCTGACACCAAAGCA







GGGGCCCCAGAGCTGTGGCTAACATCCCCTGAGCTGCAGGGGTCA







GAGATCAGCCACCATCCTGGCTGGAGTCCCACTGAGTGCCTGCTT







CTGGTCCTGCAGGGAAAGGATCTGCTACTGCAGCTGGAGGATGAG







ACACTAAAGCTAGTGGAGCCACAGAGCCAGGCACTGCTGCACGCC







CAACCCATCATCAGCATCCGCGTGTGGGGCGTCGGGCGGGACAGT







GGAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene EIF4G2. In some embodiments, the sequences from gene EIF4G2 comprise or consist of:











(SEQ ID NO: 236)



GTGGCTAAACAAAATGTAATTGTCAGTGTTTTGAGTACATTTTAG







GTTAGTCTTACAGATCTGCAATGCAGTGACAGTGCCAGGATCTGA







AATACGTTGTAATTGAATGAAGGCTTATAGATCTGTATGTTAGCA







GTGTCTAATTTTGGTGCTCTTTCTCGTTCCAGGTATTTCATTTCT







CCTGTCCCCTCCCCTCCCCACCCCATCTATTAATATTATTCTTTT







GAAGATTCTTCGTTGTCAAGCCGCCAAAGTGGAGAGTGCGATTGC







AGAAGGGGGTGCTTCTCGTTTCAGGTACTTGTAGATTTATTTTGG







GGAATCCTTTTGAAAAGGCCAGTCTCAAAAAGTAGTTAACACAGC







AAATAAGTCCGACTTAATGGGCTGCAATTTTCATTTGAAAAGTTC







TTAAGTTTTCATTGACAGCCTTCTAAGAACTGCCATCTGAAGAAT







AAATAAAATCAATTATTTGCCTTTCCTTTGGGTCCGAGTTCAGTC







TTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene EIF4G2. In some embodiments, the sequences from gene EIF4G2 comprise or consist of:











(SEQ ID NO: 237)



AGTGTTGGCCAGGCCGCCGTGCGGTCATGGCCGGATGGCCGACGC







CTGCAGGGACGAGGCCGTGGCGGACCGAGGCACGGCTGTGCTCGG







ACGGCGAGGGAGCGTGGCTCGGACCGCTAGCGCCGTGGCGGGAGG







CACGCGGGCCGGCAGGGAAGGCCAGGCCGTTGGCGCGGGACCAGG







GAACCGCAGGCGTCCCGGAGCCTGGCCTCCCGGCGGGGAGGTGTG







TAGGACCTGGAGTCGGCAGGGGCTGCCCTTCGTCGGGGAGGAAAG







AGAGAGTGATGATTTCTGCCCCTTTTGTCAGCAGAGGAGGCCGTG







GCGAGGCGGTTGTCAGTCCCGGGGGGATTTATGGTGCAGGAGGGG







GCCCGCTTGGAGGCGGCGGGTGGGCAGGAGGCCGCCTTCCTGCTT







GGAGAACAAAGAGCTGGGCGCCGGGCTGGCGGGGGTGGCCTCAGC







TGCTCCTCCCCGTCGAGCGACCCGGGCCTCGCGGGGAGGTTGTCG







GAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SYT13. In some embodiments, the sequences from gene SYT13 comprise or consist of:











(SEQ ID NO: 238)



GTAGGGGTCTCCTGCCCCCTGCACTTCCATGGGCAGCACTCCAGT







GGGGCTTGTCAATGTCTCTGTAGATGCTGTGTGTGGCCTGATGAG







GAGGCCGAGTGCCCCGGCATTTGACGCTGGCCAAGCAGATGGGCA







CTGGGCCTCAGAATTATGGTCCTTTCTTCCTGACAACATGTTGCC







ATACCACTTCTCCCCCAGACCGCCAACTTTCCACTGGAAAATGGA







GTCCCTTTCTAGTCATATGTGTGCAACTATGAGAGAGGATAAAGA







GCCATGGTTAAAACCATGGGCTTCAGAGTCAAGGGTTTAATTTGC







TGCCCTTTCATACACTTATTGTGTGGCATGGGTCATGCTGTAACT







CCATGAACCTCGGTTTCTTCATCTATAAAATGAGACTAGACTATT







ATCGAGCCTGGCACAAAGGAGGTAACAAAAAACAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SYT13. In some embodiments, the sequences from gene SYT13 comprise or consist of:











(SEQ ID NO: 239)



CAGGGAGCTCAGTGACTGGAGATGAGCAAAGGAAGGATTGGGGGC







GATATCAGAGAGATGAGCAGGATCAAGCTAGGCAAGCTGCAAGGA







GTCTGGATTTTGTGTGGTGGGAGCCACTGGCATTTCTGAGCCATT







AGGTGGAGAAGAGGAGGGAGGAAGGGAGAAGGGAGAAGGAAGAGG







CAAGGGGAGCAGATGAGAGCAGCAGAGTGGGGACCACCTGCAGCA







GGTCCAGGCAATATCCATGGGCGCAGCCATAGGATGGATGGGCCG







ATGGATGGAGGAGGTATGGATGAACAAGGCCCACATCTCAGTGGA







TTGTGGATTTGAGTGGGTTTGGAGATGATGTTAGACCTTCCTAGG







TTGTGCTCTTCAGAGCAGAATGAAACCACAACTCTCCCTCTGCAT







CCTACAACAGGTCAGTTCACCGTTTCTCCCTTCCATGGGGGGAAA







CAGACAAGAGGGCTCCTTTGTGGACTAATCAGAGTAATTTGTGTT







TGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MAPK8IP1. In some embodiments, the sequences from gene MAPK8IP1 comprise or consist of:











(SEQ ID NO: 240)



CTGCTAGGTGAACGTGTACTCCAGATCTCAGCCAGCCAGGTGGGG







GGCTGAGTGGAAGTGATTTTAGGTCCTTTTCACGATCAAGCAAAG







TGAGGCCCGGCCAAGTTGGGCAGCCAGGGATTGTGGCAGCCTCTG







TGGGCTCTGCCATTCCCCGTGCCTCACCCACCCTCCTTCACTTGG







CTGCTCAGCTCCCTCCTGCTCTTTCTGCCCCTCCTCAATTCACGC







TTGCTTTCCAGCCCTGGCCAAAAACAGTGACTGGGTGGACCAGTT







CCGGGTGAAGTTCCTGGGCTCAGTCCAGGTTCCCTATCACAAGGG







CAATGACGTCCTCTGTGCTGCTATGCAAAAGGTACCTGAGCCCTC







TCCCTTCTCCTCCCTTGGATGGGTCCCTGGGGCAGAGGGACGCAG







GTGTCTAGAGGCAGTAAAGGGCTCAGGCCCTGGGACAGGAGGGAT







CAGCAGAGGAACAGACAGCAGCTGACGTGGCTCCATTTGTCACCT







GTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MAPK8IP1. In some embodiments, the sequences from gene MAPK8IP1 comprise or consist of:











(SEQ ID NO: 241)



TGACTTCTTCCAACCCAGCCCCTTCCTTCCATGGCCCCAAGCTCT







CCCCCAAGACTTGTGATGAAGAGGCCATCTCCTGTCACCCTCACT







GCAGGCCAGGTGACCGCCCTCTTGCTTCTTTTCTCCCTCCTGTAG







GGGAATAAATGTAGCCACTTTTTCCAGTTAAAAAACATCTCTTTC







TGCGGATATCATCCAAAGAACAACAAGTAAGTGGGGGTGGGATGG







CAGTGGAGGAGGCACGGGTGGTCTGCAGCCTTGAGGTGGGTGGGT







GTGGGCCGAGCCCCCGTCCCAGCACAGACAGACCTGTCCCTGCAG







GTACTTTGGGTTCATCACCAAGCACCCCGCCGACCACCGGTTTGC







CTGCCACGTCTTTGTGTCTGAAGACTCCACCAAAGCCCTGGCAGA







GTCCGTGGGGTACGTGTACACCCTGCTGAGCACCCAGCCCGAGGG







AGCACGCCCAATCCCAGGCCCTGGGGCTGCTTCCTGGCGCTCAGC







TTTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene UBE2L6. In some embodiments, the sequences from gene UBE2L6 comprise or consist of:











(SEQ ID NO: 242)



GTGAGTCCCGGGTCCGAGCCTTCCGCGCGGGGATCGGGCGGTTGT







CCGGCGGGTCGTCCTTCGACGGCCTCCTGGCCTCGCCGGAGCGGG







TGCGGGGAGGCCCACGCAGCGACGGCTGGAGGAGGGGTGGGCGGG







GCTGCCGGGGGTGGGGTCCCCGGGGCGGGGCGGGGCGCGCTGTGT







CGCGGGTCGGAGCTCGGTCCTGCTGGAGGCCACGGGTGCCACACA







CTCGGTCCCGACATGATGGCGAGCATGCGAGTGGTGAAGGTAACC







GCGTATAGGATGACCCCTTTCTCTTGGCTCCCCTCGCCTCCCGCA







TTCCCTCCTCCCTCCATCCCCTCCGCGGCTCTCCGAGCACATTTA







TTTATTTGTCTGGGCACCAGGGCTGGCTGAATCCCTGTCCTCCCC







GCCCTCTACTCAGAGGAGGACTGGCCTGCAAGAGTGGCCAGAGCT







GGGGTTTTGGGGGTTTGGAGGCCTCTGCTTGAGCCGTTCGCGTGG







TGAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene UBE2L6. In some embodiments, the sequences from gene UBE2L6 comprise or consist of:











(SEQ ID NO: 243)



CTCCAGTCAGGGCGACAGTGAAACTCTGTCAGAAAAACAAGAAAC







AAAAAAACTGTGAGGTACTGAGATCACAAACTGGTGCTCAAAATA







TACTGATTTCCTTTCTCGTCTGGACTAAACGGGGGTAGGGAAGGG







TGAGAAGGAGGGGATCGGCCCTCCCAACAAGTCTCATCTTCCCCA







GATGCTGCTTGGTGCTCAGGGGCTGCAAGTACTCTGTCCTTGCAC







AGAGGGTTGGGGACTCCAGCTGTGCATGGGGGCACACATTAGGCC







CACAGCTTGTAATGGACCAGTAGTAAAGTAAGGGAGGGGAAGCAG







ACTCTCTCTTGGAAAGGAGGCTCCTGAGTCCTTCTTGGGGCTTCC







CCCACCAACTGTTTCCCAAACCAGCATACAGTAGGCACTTAGTAA







ATACTAAACCTAGTAAAGGACTGAATGATGGTAGACCGAACCCCC







AAAGCAAAAGAGAAAGGGTCCAACAGCGCCTCCTTGCGGTGCTCT







GCCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CPSF7. In some embodiments, the sequences from gene CPSF7 comprise or consist of:











(SEQ ID NO: 244)



GTTTGTCGGGAGGGAGCGGGGCGGGCCTAGCTGGGGGCGAAGAGT







AGGCCTGGGTTGGGGGCAGAGGCGGCTGGAGCTGCTTGACACCTG







GGGGCTCTGGCCGGAGCCGCCTTGGGGGCTAGGCAGGTGCAGCGG







ATAGGGGCTGCGGGCTTCGCCGCGGTCATTCTTCGCAACGCCGTC







CTAGGGCCACTAGGACCCGAGGGTGGGACTTTTCCGTCGCGCCAT







AGGCAGCTGCGGCGCAGACGCGGCGCTACCCGGGTCGGGCCCGAG







GGTGATGGGCAGCGACCCCCGGCGGCCTGGGAGAGCGGTGGGCGG







CGCGAGGCCGGGGGCGGGGCGGAGCGGGTGGGGCCGAGCTGGGGC







CGCCAGGATGCTGCGTCTCTGGAGATGGGAGGTTAGCAATCATTA







CCCTCTTCCCAGCCCAGGACCCTCGTGCCTTCTAATTCTTGCATT







TTTCCGAATCCCGCAGTGGCATCTTCCTTACTTTGTCCATCCTCC







GGAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CPSF7. In some embodiments, the sequences from gene CPSF7 comprise or consist of:











(SEQ ID NO: 245)



CGATGACTGATCGTTTAAGCAGATATTTCCAGACCGGCACACAGT







AGGCTCACCAAAGAATGGAAGCGACCTTTATTTCCTGAGTTGCAG







GATCGGCCTCTCCCTGGGCCTGCCCTTGCCCTGCGCTATCGGGCA







GACGAACTCTCCCCAAGATAGAGACCACAGAGGAAGACAAAGGGT







AATCCCCGCTGCCGCCCGCCCCGGACGCTAGAACGTTCTCTCTCC







TCACCAGCGACGAAGTCGAGAACACTGTAGACACCGCCATTTTCC







CCACCTGCACCGGAAACCGGCTGCGCGGGCACTTCCGGGAACTCC







TAGCCGCCCGGCCAGCCGACCACGGCAGCCACGGAGGAGCTGAGC







CGGAAGGTGCGTCCCTGCGCATGCCGGAAGTGGCGGTGCGTGTGA







CGCCGGCGGCGGGGGGGGCGGGACCTGGGGCGGAGAGCGGTTCGC







GCGCTTCGGGCCTAGTCCGGACTCGCCGCCGCCGCCGCCATATTC







CCGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene BSCL2. In some embodiments, the sequences from gene BSCL2 comprise or consist of:











(SEQ ID NO: 246)



GGACCCTCCATTTTCCATATCCAGGAAAATGTGATGCGCCACAGG







TATCAGCGTCTGGATCGCCACTTCACGTTTTAGCCACAAGTGACT







CAGTGGAAGATCCAGAGTCAACAGAGGCTCGTCAGGAAGATGTCT







ACAGAAAAGGTAGACCAAAAGGAGGAAGCTGGGGAAAAAGAGGTG







TGCGGAGACCAGATCAAAGGACCGGACAAAGAGGAGGTAGGGGCC







CTTGTGGGGACAGTCAGCAGAAATATACTGGAAATAGGTGGGCTG







GCGTGAAGGGGGCGGGGGGATGGATTGGTTTTAAGAATTTCATTC







ATTTTGAATAGATTACTTCCCACGCCCGCAGCAGTGTGCAAAGAT







GTACACAACACTGGCCTTGCCTCTGAAGGACTTTATCTGGGGTGA







TTAGACGTCTACAAAATAGAAGACTGCCAAAAGCTAGTCTAGATA







ATGTGCTGTGGGGACTCTGAGGGAGAGTGTATCCAGTTAGGGTCA







GAGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene BSCL2. In some embodiments, the sequences from gene BSCL2 comprise or consist of:











(SEQ ID NO: 247)



GTCATACCCCAATATTTTGTTCCCCTCCTATCCTCCTCCTCCTCC







TCCAGTGGACTGTCTCATCCCTGTATTCCTCATGGAAAAAAATAG







GGTGGGATGAGGTGCTCCCCAAACTCCCTCATGGCCTGGGCCACA







GATCATTAAACCTTACACAAATGATTTAAGGCTTTTCAGCCCTTC







AAACCATGTGTGTTCCCAGCTCTGAAGACCTCAACCAACCCTGAT







GAGGAGGGCACATCACCCCTGTCTGCTCCAGTGAGCCCCAACAGG







AGAAAAACAGGCCATTACTTACCTGAAGCCACAAGCGGCAGTAGC







AGCCCTGGGTGTAGGGTCAGTGGAGGGTACCAAAACCTTCTGCCT







GAAATGGGTGCCCCACCCAGTAGGGAGTTGCAGCTGAAAGTATCT







GATGCAGAAGCAGTGACTATACGAAAGGAGGCCCCTGGGGAGGGC







CAGGCCAGAGGTCTGGTCTCAGTTTCTGCAGCTCAAGCACTGTCT







GGAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GNG3. In some embodiments, the sequences from gene GNG3 comprise or consist of:











(SEQ ID NO: 248)



CTGCATCAGATACTTTCAGCTGCAACTCCCTACTGGGTGGGGCAC







CCATTTCAGGCAGAAGGTTTTGGTACCCTCCACTGACCCTACACC







CAGGGCTGCTACTGCCGCTTGTGGCTTCAGGTAAGTAATGGCCTG







TTTTTCTCCTGTTGGGGCTCACTGGAGCAGACAGGGGTGATGTGC







CCTCCTCATCAGGGTTGGTTGAGGTCTTCAGAGCTGGGAACACAC







ATGGTTTGAAGGGCTGAAAAGCCTTAAATCATTTGTGTAAGGTTT







AATGATCTGTGGCCCAGGCCATGAGGGAGTTTGGGGAGCACCTCA







TCCCACCCTATTTTTTTCCATGAGGAATACAGGGATGAGACAGTC







CACTGGAGGAGGAGGAGGAGGATAGGAGGGGAACAAAATATTGGG







GTATGACCACAGCCTTGTAAAGGTTGGTGTGGAGTGAGCATGGAG







GGGGGCCTCCAGCTGAGACTGTGCTCTGAGAGGTCCCTCTTTTTT







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GNG3. In some embodiments, the sequences from gene GNG3 comprise or consist of:











(SEQ ID NO: 249)



TAGGTGGGACCCTGGCTGGCTCCCATTAGTTGGCCAGGCTGTGCT







GTGCTGCAGGTTCCCAAAGCTCAAGATAAGAGATGCGTTCTTTCC







TTCACTCCCTGAGGTCAGGGGAGGAGTCTGGGGCTCTGTAGAGAG







AGCTGTCCCCAGGCCTCTGGGGGGGATGAGGGAGAAGACAAGTCG







TAAGGAGCCCTGGAGATGGCAAAAGAGAACCCATTTGGGGAGGGA







GGCTGCAGTCCCCTTCAGAGGTCCTGGCTAACAATACCCTTCCTG







GCTGTGTCCCAGGTGTCCAAGGCAGCAGCAGACCTGATGACTTAC







TGTGATGCCCACGCCTGTGAGGATCCCCTCATCACCCCTGTGCCC







ACTTCGGAGAACCCCTTCCGGGAGAAGAAGTTCTTCTGTGCTCTC







CTCTGAGCTCCCCTGTCCCTTCTCACAACTCCTCCCTTTTCCCTC







TCCTGGGCCCTTCCTTAGGTCAGTAATTGTTGTGAGCCCCTTAGG







CTCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SLC3A2. In some embodiments, the sequences from gene SLC3A2 comprise or consist of:











(SEQ ID NO: 250)



ACTTTCTTTATATACTTGGCCCCCTGCATTTCAGGGCTCCACCGC







CATCTGTCTGTCTGAGTGTAAAGTGGACCTTATTGGCATCTGGGC







TGCCAAACTGCTTTTTTTCCTTTGCTTTGCCCAGCCTTCTCTTGA







AGGCTAAATTCTTGAAAAATTATATTCTTTGTGTTCCTATAGTAA







ATATTAAGCTTTATATCAACACATATGGCAGGATCTGAGGCTCTT







TACTCACCTCTGGTCCATTTCTCTAGTCTTTTCTCTGTAGTCTTT







TTGTTTTCCTGTCCTCCATCTCCTAATTTATTAGTTTTATTATTA







TATTTTATATTATTTTTTTGAGACAGAGTTTTGCTCTTGTTGCCC







AGGCTGGAGTGCAATGGCGCGATCTTGGCTCACTGCAATCCCTGC







CTCATGGGTTCAAGTGATTTGTCTGCCACAGCCTCCTGAGTAGCT







GAAATTACAGGCACGCACCACCATACCTGGCTAATTTTTGTATTT







TTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SLC3A2. In some embodiments, the sequences from gene SLC3A2 comprise or consist of:











(SEQ ID NO: 251)



TGAGCCACAGTGCCTGGCAGTTTGTTTTATTTTGGTTGAACAGTT







ACAAGTCTTGGTTTGTTTTCCTGCAGCAACAGGTTTATCTTGATA







TGATACAGCGTTATTTAAATTCCTTTTCATTTTCCTGCCTTTGTC







TTCACATGCTAGTTTTTTTCAGTTGTTTCTTTTTTCTTCTTCCTT







TTCTTTTTTTTTTTTTTTGAGAGATAGTCTTGCTGTGTTGTCCAG







GTTGCAGTACAGTGGTGCGATCTGGGCTCACTGCAACCTCTGCCT







CCTGGGTTCAAGTGATTCTCTCACCTCAGCCTCCGGAGTAGCTGG







GATTACAGGTGCCCACCACCACGCCTGGCTAATTTTTGTATTTTT







AGTAGAGACAGCGTTTCACCATGTTGGCCAGGCTGGTCTTGAACT







CCTGACCTCAATTGATCTGCCTGCCTTGGTCTCCCAAAGTGCTGA







TTACAGGTGTGATCCACCGTGCCTAGCCTGTTTAGTTGTTTCTAA







GAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RTN3. In some embodiments, the sequences from gene RTN3 comprise or consist of:











(SEQ ID NO: 252)



CTAGTTTTTTTCCTTTTCAGATTGTGAGACTAACAGATTTATAGA







GGCAGTCTGTGCTCTGAATTGTTCAGCTTACAGATTGAAGTCTAT







CAACCCTTTATAGTATCAGGTTATTTGTTTTGTATAAATTATTTT







GCTTTTGTTGATTAGCTCTATCAAAGAATAAAAAACCTCCCAAGA







TTTCTCAATCTTACCGCCCTTCTTTATGCTTTCTCTGTACAATGG







AAAAGTAGACCAAAAGTATTTGTCATGAAAATTTTTTATCATTTG







CAGTTTGTGGCATGATATGGCTTTTCTGTTTCACTGTTGGCAGTT







TTATGCATGTCCTGTAGGATAACTTGATCGTTTTGTTACATATTT







ATGTGTGTATATATACATCCTGTGTGTGTATATATATATTTATGT







AATTTAAAAAATAAAAAGCATGCTGCTTGGCTTGGCTAGCTAATG







TGTAATTGTACCTGGTAAACAATTTTTTTCTTTGAAATTCTGATC







ATAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RTN3. In some embodiments, the sequences from gene RTN3 comprise or consist of:











(SEQ ID NO: 253)



CAGTTTCCCAGAGCATCCTGCTTTTCTCTCAAAGAAAATTGGTCA







AGTGGAAGAGCAAATAGATAAAGAGACCAAGAACCCAAATGGGGT







ATCAAGTAGGGAGGCTAAAACTGCATTGGATGCTGATGACAGATT







CACTTTGCTGACAGCCCAGAAACCACCTACTGAGTACTCTAAGGT







AGAAGGCATTTATACATATTCTTTGTCTCCATCCAAAGTTTCAGG







AGATGATGTTATTGAAAAGGATTCCCCTGAATCACCATTTGAAGT







AATTATTGACAAAGCAGCATTTGACAAAGAATTTAAAGACTCATA







TAAGGAGAGCACAGATGATTTTGGTAGCTGGTCTGTGCACACTGA







TAAAGAATCATCCGAAGACATTTCAGAGACTAATGACAAGCTTTT







TCCACTGAGAAATAAAGAGGCAGGACGTTACCCAATGTCTGCATT







GCTCAGTAGGCAGTTTTCACACACAAATGCAGCACTGGAAGAGGT







GTCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NRXN2. In some embodiments, the sequences from gene NRXN2 comprise or consist of:











(SEQ ID NO: 254)



GTAGGCGGCGCGGGCCCGCGGAGGAGGCGCGGTGGCGGGGCGGGT







GGCGGCCTCTGATAGGAGTGCGGCCGCACCGGGGCGGGGCTAAGC







GGCTCTGGGGCGGGGCTAAGCGGCTCTGGGGCGGGGCTACTGGAG







AGACGAAGCTTGCGAGGCGGGACTAGGGCAGGGGCGTGGCTAGGA







AAAGTCGGGATTTGTGGCGGCGGGGCGAGACCGTGGTGGTGCGAA







TGCGCCCCCTATGGGGGAGGGTGCAGGGCTGAGCTGGGAAGGTTG







CAAGGCCAGGGTCCAGTTTGGGGGTGGGGGGAGAAGTGGAGGGAG







GGTAGAGGTGAGGAGAATCTGATGCGATTGCGGAAAGGTTGAGTT







CAGAAATGAGGGAGACAGATGCAGAAACAGGAGAGAAAATCCAGA







AGTAATATAAAACGTGTCTTGGCAAGGAGACAGGGCTGTTACCAC







GGGCAGAGCCAAGGAAAGAACCGAGGTCCTTATAAAGAGCAAGTC







AGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NRXN2. In some embodiments, the sequences from gene NRXN2 comprise or consist of:











(SEQ ID NO: 255)



GCCTGCTTGGTACAGAGCATACCAGGGAGGAGAGGCTGGAGACTG







GGGACTGGGGAGGGGCTGGGGGTCAGACCTTCCTCTGGCAGAGGC







CGGCTTCTGTGGACTTCTCTGCTGATCTTGCCTTAGAGAGGCCTC







TGCCCTCCATGCCCCGAGCTGGTCCCCAGCTTGGCCCGATCACCC







CTGTCTGGGCCCAGTGGCCCTTGCACAGAGTAGGTGCTCTGTGAA







TACTTGTGAATATCCAGGACAAACCAAAAGGCAAGAGACAGGTGT







GGTTAGTGGAAGGCGCTCTGGGCCCAGGCTCTGCACGCACCTCCG







GCCTCAAGTTACTCCCTTGCATGTGGGGAAGGGGAGCGAGCCTGC







CCACCTCCTCCAGCGGTTGAGACCTGGGAAAGGTGCCTTAAGTGC







TGCGGAGCTGGGCTAGTGGTGGTCACAGGAGTAGCCACTAGAAGG







GATGAATGGTTGCTCCCTCTGGTCGCTGACCTCCACCTCCATGTC







CCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SF1. In some embodiments, the sequences from gene SF1 comprise or consist of:











(SEQ ID NO: 256)



GTTGGCATGCTTCAGATGATGGTTGGGTCTGATCTTTCTTGGTCT







CTTTTGGGGAGAGAATTTTAGTTTGTTTTTTTTTTTAAATCTGTT







CAGTATGTTTAGATCTCAGTCTGATCGGCTGCCCTGTCGCTTTGT







TTTAAAAAGCTTTTCCCTCTGATCCTGTACCCCTTGGGAAATAGA







AGGACTCTTTTGTGGCAAATGTAGGGAGTTTCTGCCTATGTACGG







GGAAAGGAGAACTTGGCTGCTATTGTATAGGTTGTTGAATTTTTG







ATAGATCCTTTTAAGAGTTCAGACCCTCAGTGTCCAACTGGCCAG







GAAAACTGAATGCTGATCTTGGCTAAACTTATGAAGACAAAGGTG







ATCTTGTTGAAGAATTGGGCTTTTCCCACTTGGTAGGGTGTAACT







AGGTTTAGTTGTGGTCCGGCCTCTCGTACAAAAGTGACTGGTGAG







GGGAGTGGAGGTGGGTGGAGCCCCCAGTCCCATCAGGACTGCTTT







ACAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SF1. In some embodiments, the sequences from gene SF1 comprise or consist of:











(SEQ ID NO: 257)



CCCGGGCCGCCGCCGCCCCCGCCCGTGGGCTCGATGGGGGCCCTG







ACCGCGGCCTTCCCCTTCGCGGCGCTGCCTCCGCCGCCTCCGCCG







CCGCCCCCTCCGCCTCCCCAGCAGCCGCCGCCGCCTCCACCGCCA







CCGTCCCCCGGCGCCTCGTACCCGCCGCCGCAGCCGCCCCCTCCG







CCGCCGCTCTACCAGCGCGTGTCGCCGCCGCAGCCGCCGCCACCC







CAGCCGCCGCGTAAGGACCAGCAGCCGGGCCCGGCCGGCGGCGGA







GGAGGTGAGTCGGGCCGAGAGAGCGCGAGAGGCGTCGCGCGGACG







GGCCCGGCCGCCTGAGGGGCGCGCGGCGGCGGCGACGGCGGCTGC







ACGCGGCAGGGGCGCGCGTGCGCGCACGGGGAGGCCCGGGCGGAG







GCGCCCCTGGGCGCGCGCGGCCTGGTTCTAGGGCCTCGAGTGCGC







ACGCGCGCTGTGGGGGGAGGGCTGGGCGGGCGGGAGGCGCTCTGT







GCGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TM7SF2. In some embodiments, the sequences from gene TM7SF2 comprise or consist of:











(SEQ ID NO: 258)



TGGGGGCGGGGGACTAAGATGGACGCCTGGGAAGGGAACTGGGAG







GCAGCGGGGTGCCTGGGGGCCGAGGGCTGAGGACGGGGTGCGGAG







GCGCACTCTGGGAATGCCGAGAGGGTCCCGCAGAGACGTCAGGGC







GCCCGTGCGGGCCGGCGGGGAGCTGGGGGGCTAGGGGCGGACGCC







CGACGTGATGGCCCTTCCCGCAGGCGCCGCGGCTCTGCTACTGCT







GCTGCCCGCCACCATGTTCCACCTGCTCCTGGCGGCCCGTTCGGG







CCCCGCGCGCCTGCTGGGTCCACCCGCGTCCCTGCCGGGGCTGGA







GGTGCTGTGGAGCCCACGGGCGCTGCTGCTGTGGCTCGCCTGGCT







CGGCCTGCAGGCGGCGCTCTACCTACTGCCGGCGCGCAAGGTGCG







GGCCCCGCTCGCGGACGCTCGGGGGAGGGAAGCGAATGGGCTCGG







CGAGGGAAAGGACGCCCCGGGCCTTATCAGAGCCCCCTTGGACCC







GCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SPTBN2. In some embodiments, the sequences from gene SPTBN2 comprise or consist of:











(SEQ ID NO: 259)



GTGAGGGACACAGGACCCCGGATGCCCACTCCAACCTGCTCCCCT







GACCTGTGCTGGCTTCTGCTTGGAGAAGACATGTCTTCTCTCTTC







CCACTGCACCAGTGGCCCCCAGATGTGAGCTGAGAGTGCCATTGT







CACCACACCTTTAGGAGGTGTCAGTTCCCCTGGGTGATGCCGAGA







CACCTTCTCCCGCTTTGTTTCTTGTCCCCACCATCTTCTCAAATT







CTGTTCTCCTCTTATCATATTTCATCAAATTAGAGTTGAGACATT







TCAGCTCTCCCTGTTTCCCTGGCCCTCTTACACGCAACCTTCTAC







ACGCTGAATGCAGCATTTTTTGACAGCTAGAGGAGCGGGAGAAGG







AGCGAAAGAGAAAGAGGGAGGAGGAGGAGCGGCGGAAACAGCCGC







CTGCTCCCGAACCCACAGCCAGTGTGCCTCCAGGGGACCTGGTGG







GCGGCCAGACAGCTTCTGACACCACCTGGGACGGGTGAGAGCCAG







GATG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SPTBN2. In some embodiments, the sequences from gene SPTBN2 comprise or consist of:











(SEQ ID NO: 260)



TGAGCTGAGATTGCGCCACTGCATTCCAGCCTTGGTGATAAGAGC







GAAACTCCGTCTCAAAAAAAAGAAAGAAAGAAAGAAATCACCTGT







CCCCCTGGATGACTCCCCAGGGGCCGTGGAAAGATCTCACATCCT







GGTGCTAACTCATATCTCTGCCCCCCGCCCCCAGATCTCAGAGAA







GCTGTCTCAGCTGCAGGCACGGCGCCAGGAGACAGCTGAGAAGTG







GCAGGAGAAGATGGACTGGCTTCAGCTGGGTGAGCTGCCAAGGGG







GCCCCAGGCCCTGTGGGGAGTGGGGGGCATCCTGCACCCTGTGGG







TTCCAGAGTAGGTGAGACTAGGAACCCTGGGTGTGAAACTCACGA







TGCCCAATCTTCGTGCTGCCTGGCACAGCTCTGGGGCAGTGGCTT







TCTCTGTGTCCCTGTTCTTGAGGTTGCCATGGCCACTGTGCCCTG







CCAGTGGCCACTCTGACCCACCATCTTCCTGCAACCCCCGATCCT







GCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PC. In some embodiments, the sequences from gene PC comprise or consist of:











(SEQ ID NO: 261)



GTGAGTGTCCGCCGCGCTCCGCACGGTCCTCTTCCCGCGGCGGGT







CGGAGCGGGTGGACGTGCGCCGGCACCGCTGCGAGGGACGCTTAT







GTAAGGGTCGCGCGCGCAAAGGCGGCGCGGGCTCCGCGAGTGACG







GCTGGAGGAGACAGCGACGGGCTGTGCGCGGTGCGCGACGCGACG







CAGCGGACGGCGCCCCTCCCCCTCGCGCGGGGGCTCGGCCGGCTG







GACCCGCCCCCGGACCCGCCCCCGGCTGCAGCCCCCGAGCGCCCC







TCCGCAGCGTCCGCCTGCGGGCTGCCTCATTCTTGCTCTCGGGCC







CATTTTGCAGATGGAGAAACTGACTAAAGCGCCTCGCGCGAGGTC







CCTGAGGGTTCGAACCCAAGTCTGTCAGGATCGGGAGCCCAAGTT







GTCCCCATCGTACTGGATGTGGCTCAGCTCTGCAGGAAGCCGCGG







CGTGTAAGGCGAGGGCTTTCCTTGTTCCTTGCTCTCGCACTCCGC







CTCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PC. In some embodiments, the sequences from gene PC comprise or consist of:











(SEQ ID NO: 262)



TCTTCTCCGCTAGCCAGCGCGTATCTGCGTCTAGCCGGGATGCCT







GAGCCCCAGTGCGAACTGTTGTGCTTGCAGCTTCGGGAGACACGT







GCTACTCGGGTGAATGAATCGAACGTCCCCACCCCCGCCGACAGC







CAATAACTGCGAGCCACAGCCCGGCCACTTCCGCCTATTGCGGGC







GTCGGCTAGGGTCCGGCGGCCCACGTGAGGCTCCGGAGACAAGGG







AGTAGGCGGTGGTGCGCCAGGGGCGGGCTCTCCCAGCCTCGCCAC







TTATCCAGGCGCTCGCCGGGGACGGGAGGGGCGGGGCTGACGTGG







GGCGCCAAGGCTTAAACGTGACGGACAGGCGGGCACAGGGAGGAG







TCCAGAGCCTAGGGGCGAGGAGTGACGGAGAACACTGCCCAATAA







CGGGAGGGGTTGGGCTGTCTGGGCCAATAGGAAGTCCGTAAGGCG







GGGCCGGGACTGCAGCAAGTTCGGTTGCACGGAGACCGCAGCTGT







TCTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ANAPC15. In some embodiments, the sequences from gene ANAPC15 comprise or consist of:











(SEQ ID NO: 263)



GTTAGTTCATCCATTCAATCTTCCATCTACTTATTTATCCACTTA







TTCATGCCTCCTAGAATGTCTTACTGAACCCTGCTTGCATATGCT







ACAATCCCTCCACCCCGACATTTGGCTGTGATTCATGAGAGGTCG







TTAGTGTCCTAAATTTCTGACACTGGGTGGGTGTGGTATGGGCCA







TCATAAAGGGTCAAAAAGCAGGTTTTAGTGTGTGGAGGAGGTAGG







GATAGTTTCAGTAGAACCCTGCTACCTCTAATTTTGTATAATTTC







ATAATGCCAGTCTGCATTCTGGATTCCTATCCCTACACTTGACTC







CCATTCTCATCCCTCCTTGAACAGCCTGCAAACAGCTTTGTTGAT







CCATTATTTACTTAATTATCAGAATAACTTTGACAGTGGTATAAT







TGTTATTATCCCCATTTTACAGATGAAGACACTGAGGCTCAGAGA







GGTTAAGTGACTCAGCCAAGGTCAAACAGCTAGTAAGTGGTGGAG







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ANAPC15. In some embodiments, the sequences from gene ANAPC15 comprise or consist of:











(SEQ ID NO: 264)



TGGGTTCTTCCGCTCAGGCTGGGTGAAGCGCTTCCGGGTCGCCGC







CGGCAGCAGCCTCCCGGCGCGGTAAGTACAGACGCAAGGCTGCAA







CCGTCTCCTTGCCCCTTCATTCCTTTTTCCCCCAGAGAAACCCAT







TTCCTTTGACCCGACTCCCCAGGAGCTCCATATTGAGAGTCCTTA







ACCCTTAAAGAGCTCCAGCTCATTGGCCGCTTCCTCGAGACTAAG







CCCCACCCCGTCACACAAGGTCCGTCATTCATTGGTGTCACTTCC







TGTCACCCAGCATTTCTTTCAGTTAATTGGTCCTACCCAGGCCCC







GCTTTTTTCGCGTTTAGCAACCCTATAAGGCCAAATTTCTGTCTG







AGACCCGCCCTGCTTTCTTTCTGTTGGTCAACTGCATGGTCATTA







TCGTAATGAGCAAAGAAAGGGCGGTGGCTTTGCGACGGTGGGTTG







GGCTTTCGGAGCCGAGATCGCTGGTCTGTCAGTACTTCTTGGACA







TGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MAP6. In some embodiments, the sequences from gene MAP6 comprise or consist of:











(SEQ ID NO: 265)



GCAATGTTTCTCTCTGTCTCTGTTGGGCAGGGGTGCATACATGTG







TATAGGTGGGCCTGTCAGGCTCTGCATTCACCCGTCTGGACCAGA







GGGGTCTGAGAAGCACCAGTTCCTGTGAGTTGTCAGCATGAGATG







GGAGTGGGTAGGCGATGCTAGATGGAAACAGGACCCCATCACGGA







GGTGGGCAGGATAAGGCAAGGGTCAGATACCATGACAGCCTCACA







AGAGGTGCCAAATCCCAGCAGGCTGAGCCCTGGGTAGCTGAGTTG







AAGCCTGCAGATGAAACAGGAGCAGGGACCCACCTGGCACAGGGG







AATTCTGGATGACAGTTGTTTGCCAAGAGCTCAGCCTTAAAGCTC







ATCCAGGGGTCAAACTTCTTGAGGAAACCAGGCTCTGATCATAAG







TCTCAGGACACTGTATAACCTAACACCTGCTAATCCTCATTCAAT







ATCCATGACAGCTCAACTGAGAGCTTCTAAAGAATATACCTAGAG







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MAP6. In some embodiments, the sequences from gene MAP6 comprise or consist of:











(SEQ ID NO: 266)



CTTAACAAGGGGGTCAGGGAGTCAGGGAGGCAAAAGAACTTTGAA







GGCCCTGAGAACAAGAAAAGAGCACCCTGGCCTGTGGGGGCATCT







GGTGGGCAGCTGAGATGGGGCTCCCAGGATGGGCCCAACGATCCT







AGAAGAAAAAGTAGGGACCTGGATGGAATGTGGGGTGGACGGGCT







GCTGGGATGCCTACTGGATGACTCCGAGTCCAGCTACAGTACTGC







CATTGTCACCAGCGTGCCACCCCCTGTGCCACGTGCAGGTTATAA







TCAATTGGCAAGCACATTGAATGACTGATTCCTGAGCCTTCACCT







CACCCCCCTCCCTCACCCCCGCCCACATTCTGTACTAAGAACACA







GAAATCATATTCTCCTGGCTCTCCAGCAAGAGCTGCCCGGCCCAG







GCTCCACTAGGCCAAGACTTATTAGGCAATCTGTACTCTGCCCTG







TCCCTGCCTGAGCTCTACTCTCACAGTGATCACCTCTCTCTCCTT







TGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GAB2. In some embodiments, the sequences from gene GAB2 comprise or consist of:











(SEQ ID NO: 267)



GTGAGTTTTGGGAAACAAAGAGTATGGCGGGCAAGTACCTAGCAG







GTTCTGAATATTTGTTCTTTAGGAGTCACCATTTAAACAAGACCC







CAAGAGTGACATTAGGTGTAACCAAAGTGAAATTGTTGACTACTT







GATGAGATTCGTGAGGATAGGCATTAAATGATATCTTTTAAGAAA







CCATTGGCAGTCCTGGAGCTGATGACCCTAGTGCAGGATCATTAT







GGTTGCTTCTTTCATCATCTCAATTTGATGACATATTTGAATTGA







ACTTAAACTATACCTAAGCCTTAATCCCAGGGTCTACTAGTGATT







CAGCTCAGCACATCTAAAGGCAATGATTATTCAAGAGGATTACAT







TGGTATCTCCTGTCACACCAGCATTTAGTATTCTATACTTATTCC







CTAAATATATCTCGTTGTCATTCTCCGATATTTACCATTTACCAA







TATTACCAATATGCCTAGAGCATAGCAATTGCTCAATAAAAGTGT







ATTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GAB2. In some embodiments, the sequences from gene GAB2 comprise or consist of:











(SEQ ID NO: 268)



AGGAGGGAGGGTTTATATTGTATTTTAAAGATCAAGGACAACAGC







CTAGAGGAGGTGGCATTTAAGGTAAGCCTTGAAGAAGGAGTTTAA







TTTCAACAGAGAGTAAGGCTGGGAATAATGCGATGTGAGAATGAT







CTTGAGAAGAGGCACAGAGGGTAGGTTCAGGGCAGATCCGTTGAT







AGCATTCACTAGAGTGTAAGCAAAAGGTGAGGAAAACTGAAATTG







TAGGTTGAAATTTTTGTTTCTGCGAGTATACTTATTTGCATTTGC







ACAGAAGGTGGTGATCCTTATATTTATTTCTGCTGTGCATCAATA







GGGGAGGTGGAGATAAATAATTTCATTGTTTTGGCTCTGGCCAGA







ATTCATTAGGCACAGTTTGTTGTCCATAGGGAGGGGAACACAACA







CACACTGGCCTTTGTCTCCCTTCCACACAGGCTGGTGGAGATTCT







GAGGCATTTTTAAACATTGCACTTAGCCATTCAGATGATAACTTG







GATC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene BACE1. In some embodiments, the sequences from gene BACE1 comprise or consist of:











(SEQ ID NO: 269)



GTGAGTCTAGGGATGTTTTGCTGAGCAGCCTGAAAGCAGACTCTA







GGATTCCCCTGAGATGAGATAAGGTGGTGTTTGGGAGTAAGTTTA







ACTTAGATCCTTATACCAGTTGTGGTTATGCATCCACAGATCTCT







GCCTTCTTTCCACAGGTCCAGCACATACCGGGACCTCCGGAAGGG







TGTGTATGTGCCCTACACCCAGGGCAAGTGGGAAGGGGAGCTGGG







CACCGACCTGGTAAGCATCCCCCATGGCCCCAACGTCACTGTGCG







TGCCAACATTGCTGCCATCACTGAATCAGACAAGTTCTTCATCAA







CGGCTCCAACTGGGAAGGCATCCTGGGGCTGGCCTATGCTGAGAT







TGCCAGGGTGAGAGGAACAGGGCTTGGCCCTACACACTCTATGCA







CACTGCACTATACAGAGAAGGAATTAGGAGAGAGAAGGGGAATTA







GCAGAAGGAGGGCAACAATCAGTTTAAAGGATTATTTAACAGGGG







TATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene BACE1. In some embodiments, the sequences from gene BACE1 comprise or consist of:











(SEQ ID NO: 270)



AAGAAGAAGGGATGAGAGGACACCAATATCTGAGACATCAGTGTG







TGCAAACGTGAGTAGAGGCTTAAATCCTATCCTTGGACTTGTAGG







CAGCTGCAAAACGCACCTTCCCTGTTTCCTCTAATGTCCCAATCT







AAAAGAAGCAGTCAGGGAGGAGGGTGCAGGTAGGATCGATGAAAG







GGCATTCACTCCAGTTCCTCGTTCCAGTTCCATCACTGCCCCCAC







CACACACAGAAGCCCTGGTTAGTGGTGTGATTAAACACAGGTGGG







GCTGCCACATGTTGAGCACGTTTCACAGAAGTGGTGTGTAATACC







AACCTGCTTTTCTGAGATGCTTGAACAAACCCCTTCTCTAGAACT







TGCTGTAGAGGTTCCACATTCACCCTGCCTGGCACTTTCTCTACC







ACTCGGATGGTACCACTAGGTTCCAAGGCAGTAAGAGAGTCAATG







GTTCCTTTTCTCGGGGAGCTTGACGGAGCCAGAGGGCGGAAGAGA







TGCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene FXYD6. In some embodiments, the sequences from gene FXYD6 comprise or consist of:











(SEQ ID NO: 271)



GTAAGGACCATCCCCCCAGCTCCCCACCTGGCTCTAGCTGAAAAT







GACAACTACAACTGGAGCTGGCTCCTGCGCCCACGACACAGCCAT







TCATGCTTTTGGTGCACATTCCCCTTAATTACCAAGGCTCCCAAT







TAACGTGGGCTGCTGTGTCTGGTGCTGGGTTATTTTCTCCTATTT







TTCCGTTCCTCGGGGTTTACTATTGCAGAGAGACACCAGGTCTGT







CATTGTGCCTGTCTTGCTCCCTTCCCACCCTAACCAGAGGGAAAC







CGAAAAGCAACAGCTGATGGCAAATCCGCTCCGGCGCTTGCACGC







CTCCTCGGCGGCAGTCTCCTGTTGCTTCTCGGAGCTTGACTGCCC







TTTGGAAAAATCTCTCCGCCCCTCGAGAGGAGAGAGGAGCAGAGG







ACGCGCGCGAGCACAGAGCGGGAGGACGGACAGGGAGAGGAGGAG







AGGGGGTCTGCGCGCGGCCGCTACCCAGAAGCCAGCGGACGGCAG







CACG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene FXYD6. In some embodiments, the sequences from gene FXYD6 comprise or consist of:











(SEQ ID NO: 272)



TATATTTCCTGCCTTGGGGTTTTGAGACCTGACCTTTTATTAAAA







TAATTAGATCATCTAGGGCAGAGCTGGAAGAGACCTCAGGATCAT







CTAGTCCATCTCCCTATTTTCCCAAATGAGAAAACAGGCCCAGAA







CTGAGGGCAGCAGAGGCAGGGCTAGGTTTCTTCCTAGCAGTACCC







ATCATCCCCGGCTTGGGGTTTGTAAGGAACTCTCCGTGGCCCTAC







CTAGGCTTGGACAATCACAGCCTTTCTCAGTAGCTCCTCCCTCCA







CCCGGCTCGGCCATACACACACCTTGACTGAGGCTGGACAGCCTC







CATCTCAGCATGGCTCCTGGCTACTGACACTTGTGCCTGGCCTCT







GCCCCTTCCTACAAGCTCTGCCCCTAATAGTAAGAGCCAATTTCT







TGAGCACTCACTATGAGCTAAGCCTCCCATGGGGTCAGTCTCTAA







TCACCATTTTCCCACGTGAGGAAATAGACTCAGGAGCTGAGTGCA







GAGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PHLDB1. In some embodiments, the sequences from gene PHLDB1 comprise or consist of:











(SEQ ID NO: 273)



CTGGGTACCCTCAGTAGATGTTCTGGAGATGTTGAGAGCATGTTG







TAGGGGCTTCGATTTGGGAGGTTCAGTGGGAGCCCGTGGAGGTTT







CAGGCATCTAAGGCAAAAAGAAAGAGGGGAGAGTTTGGGGGAGGG







AGAACGGATTGTCAAAAACACCCTCTCAAAAGCGTGTTAAAGCCT







GGGAGTGGAGGGTGGTCTAGGAGAGTCGGGAGGGCAGGGTGGGAG







GAGAGGGGGATGTGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGT







GGTGGTGGTGGTGGTGGTGGTGGTGATGGTGGAGAGCCCAGCTGC







AAAGATGAACAAAGCGGGCGCTGTGCAGGCTGGGGGAGGTGCTGC







TGTGGGAAAGGGTGGGTGGGGGGACCTTGGGCTCCAGCCCCACAA







GGGCGTCTTCTTTGGCTGGCCAGGCGGACGGGACGGGGGACGGGG







GCGGGGACTGGCGGGAGGGGGGCGGGACAGGCTCGCTCTCCTCCT







ACTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PHLDB1. In some embodiments, the sequences from gene PHLDB1 comprise or consist of:











(SEQ ID NO: 274)



TAAGAGCGCCCCGTCCCACTACACTTGCGGGTCCCATTCGCTGCG







GTGCTAGGACTGGATAAGGGGAAGTCCCCGGGGCCTGGCGAGAGC







CCTGAGATCAGCTCTAGGCTAGGGAGCTCGGCAGAAACCCGTGGG







GGAGAGAGGGCACCCCAGGTGAGTGCGTGGGGGCGAAAGGAGCGT







TATGGGGAGTTTTCCTGTGAGCCTCTTGCTTTTGCCTTCCTCGGC







CGCTGCCAACGCCACCGCCAGAGGGGGCCGTGACGCTCTCCCCCC







CTTGGAGAAACTCTGATCCTGGCCCTATCCAGACTCTCCCGCCGG







CACCCAGGCGGCCGGGCGGACACTCGCGGGGTATCGGGCGGCGAG







CGCGCGCGCGGGTTTTCGTCCTCCAGCGCCCCACACCTCCCCCTC







TCCGCCCACAGCAGGACCTTGGGGCGGGGGTATCTCCTGTGACGT







CTCCCCGCCTGTCCCCAGGCCGGCCGCCTTGATGGACCAGGAAGG







GATT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NRGN. In some embodiments, the sequences from gene NRGN comprise or consist of:











(SEQ ID NO: 275)



ACCCAGTGACCCCACAAGAACCCCCCTGCTTCGCCCCCAGGAGAA







CGCCTGCTCCAAGCCGGACGACGACATTCTAGACATCCCGCTGGA







CGATCCCGGCGCCAACGCGGCCGCCGCCAAAATCCAGGCGAGTTT







TCGGGGCCACATGGCGCGGAAGAAGATAAAGAGCGGAGAGCGCGG







CCGGAAGGGCCCGGGCCCTGGGGGGCCTGGCGGAGCTGGGGTGGC







CCGGGGAGGCGCGGGCGGCGGCCCCAGCGGAGACTAGGCCAGGTG







AGGCGGGCGGCGCGCGGCTGGCTGACAGCTGCCCTTCCCCAGCCC







TCCCCAGGAGCAGGGGGAGAATAAGGGCGGGTTGGAGGTGCAGGG







GGCGTGGAGGGAGCTAAGGGTTGGGGGATAGAAATCCGAGATGGG







AGGTGGGTGGGAAGAGGCTGAAGGTTGCGCGGATTCCAGGAGCTC







ACCTGTTTCTCCCTCTGCATCCTCCCTTCTGCCCCGCCCTGGACC







GAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NRGN. In some embodiments, the sequences from gene NRGN comprise or consist of:











(SEQ ID NO: 276)



ACTGAGCATTTTCAAAGGTAATGAACACGGCCCCTAGGCGGCGGG







GTGGGACCCGTGAGTGGGAGAAAAGGATCGAATCCCCAGCCCTTC







CCTCTGCCCACCCTCACCCCTCCCAGCCGGGGAAAATGCACCCTG







CCCCTGCGGGTTTTCTGATACATCCAGACGCCCGAGGCTGCGACG







GGGACATACCTGGACCAAGACATGCGCAGCCACCCCATTTGGCTG







CACAAGCCCGGCCAAGGGGCAGAAATACATCGCGGCGCGCACACG







TACCTGCCGCGGGGCTCATTTCAGCGCTTTGACTCGGGGTGGGGG







TTCTGGGTGACTGGGGCCCTCTGGCACTCACCGCCCAAAGAATCC







TGGTACTGAAAGGGGGAAGGGTGGGGGGAAGAGGAAGTAGCAGAA







GAGGGCAGGTTCTCAGACCTTTTCTCTCCTCAGTTCCCGAGGAGA







GATGGATGCCGCGTCCCCTTCGCAGCGACGAGACTTCCCTGCCGT







GTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TPI1. In some embodiments, the sequences from gene TPI1 comprise or consist of:











(SEQ ID NO: 277)



GGGACTCAGTCCCTGCAGTTCCTGCCAGGCCTTGCCAGCCGGGGC







GAGGGTTGGGATGATCCTGGCGGCCTATGCCTGTGTGGGCTGCCC







CTCCCGCTGTGAACCCTGCATTTGTCCCGCAAGTTTTCACTCAGG







TAGACTCCCTGGGTACAAGGGTGCCTGCTCAGCAGTCGGGCATGA







GCTGCTCCGATGGGCGAAGGAGGTTGTCTATCCCACAGTTGGAGA







GGGGCCCTCTCTGCCCCAGTGGGCGATCTGGGCTACGGCCAAGTT







GCCACCAGCTAGTTCCGCTTGAAAACCACTTCTGGCCCCGTGGGG







GACTCAAGTCGCCAAGCGAGGGTTCCCCTGAGCGCCGGAGCTCAC







AGGTCTCGCCTTGTCCCGAAAGCCCCGCAATCGAGGCGGAGGCGA







CCGAGCCCCCGACTCTCCTAGAACGTTGCCACAAGAAGGGGGAAC







GTCGGAACAGTGCATCATCGGGCGGCGGCCGGGGCGGCGGCAGGA







GGGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TPI1. In some embodiments, the sequences from gene TPI1 comprise or consist of:











(SEQ ID NO: 278)



CGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCC







CTCCAGGAAGTTCTTCGTTGGGGGAAACTGGAAGATGAACGGGCG







GAAGCAGAGTCTGGGGGAGCTCATCGGCACTCTGAACGCGGCCAA







GGTGCCGGCCGACACCGGTAAGCCCTCGCCGAGGAGGGGTCTGGC







CGGGCCGGGGCCGGGGCCGGGGCAGGAGTGGCAGCGCCCTCTCCC







GAGGCCCCGAGGCCCCGAGGCCGGTATCCGCGCGGACCTGATGCA







GGGCTGTGGGACGAGGGCCGCTGGGGTCCGGGCAGGGGCCTCGCA







GCCGCAGCCCCGTCGGTGCGTCGAGGGGGCAGGGCGGAGCACATG







ATGCCCCTTGGACTATGGGGCAGGTAAGGACGTTTTGGGTCTCCT







GGAGGAAGGTGGCCCCGGGGCGCGCACTGGGGCTGTGCCCGCCAG







GCGACGGGGTTAGGAGCGGAGCCCGAGGCTCTGCGGGAGACCGGG







GGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ZNF385A. In some embodiments, the sequences from gene ZNF385A comprise or consist of:











(SEQ ID NO: 279)



GTATGAAAGGGGGTGGTGTCTGGAGGGAACAGGAAGGGATCCTGG







AGACCTTCTGCTCTAGTAAAGTCAAGGGAGGACGGGCCAAGCACG







CAGCCTCCTCGGTTTGCCTTTGTTCCTGGGAACCCCTGTTGCTGT







CAGGGGGTTACTGGAGCAGTGAAGGGCGGCCAGAACTCCCCAGCT







GGTGCACAGCAGGGTCGCAGAAGGGAGCTGGGCCAGTGGAAGCCC







AGAAGGGCTCTGAGGACCTTTGGGCTGCCCCTTTGGAGTCTGGTG







GAGTTCCTCCCGTGACTCTGAGAGGAGGAGGAGAAGGTTTCAGAG







AAGGGTAGTTGAATGGCTGGCCTGGACTTTCATTTCCCCTTGGGG







TCAGACCCAGGCATTCCCCGCCCATAGGCCAGGTCGAGGTTCCTC







CTGCAGAACTGGACCTGTAGCTGAGCATTCCTGGTGAGACAAAAG







GGCATGGGGGAAGACTGAGGAAGGTTGGGAGAAGGGGCTCTGAGA







ATGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ZNF385A. In some embodiments, the sequences from gene ZNF385A comprise or consist of:











(SEQ ID NO: 280)



ACTGTCCGATTCCTCAGTCACCTATTCCTGCTGCTACCACTCTCC







CATCCTCTCACTCACTTTCATCTGTCCGTCTCTCTGTGTGTGTGT







GTGTGTGTGTGTGTGTGTGTGTGTGTCCTCTCTCCACCCCATCCC







AACCTTGAAGGTGACAGAGGAGGGAATAGACACAAAGCATACAGA







CCCCCAGCTACTTAAGGGTCAAAGCCAAGGATCTTACCCTCTCCT







TTAGCCCTGAGAATTCTGAAGCCCAAACTCCCAGTAGCCTTGACC







CCCTGCTCTGTCTCAGTCACCCTGACCACCCATCCTGGCATGGGG







TGAAAGAGACAGACCCCTCCAGACCTTTCTGGACCAGCTCCCAGC







CCCCAGCTGTGCCTGAGCCCTCCCCACCCCCTCCCTGTCGCCTCA







GATTCCCCTAATCCCTGCTCCCCTGCCCTCCCCTCTGCCCCACTT







TTCCAGGATTCTGATTGGCTGATTCTACTGACTGGGCTGAGGGGG







AGGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DTX3. In some embodiments, the sequences from gene DTX3 comprise or consist of:











(SEQ ID NO: 281)



ATGGGCTGCTGGGTTGGGGGCCATGGGAATTGGGAGAAGGGGTTT







CTCACTTTCCTTCCATTTTTCCGCCCTACCAGGTCACACGACCTA







CAAGTAGGGAGCAGGGAAAGAAGAGTGAGCCTGCATGCCAATTCT







AAGCTCTTCAGGATCAAAGTAAGCAAAAAGGAGGGGCAAAGAGTC







TCCCTCCCCTTTAAACAGCCCATTTTTCCCCAAGAGGTGGGAGGG







GCAAGTTGTTTCTATGATCTGGGAGAAATGACAGGAGAAACAAGA







CTGATACTTGGCTTAGATGATAATATAGCTCTGGAAACTTTTTTG







CTCTTCAGTGGGTTTTGGGTTTGGAAGATAAGGGGAGACAGAATA







TGAATTTGGGTGAAGGGTTGGGGATTAGGCTTTGAGAAAACAGAG







GTAATAGAGGGTTTGGAAGGAACCTGAAGTAGGAGGCACTGATAC







TTGTCAAATGGGGACCCCCCACCCTGAGTCCCTTTCACCCTGGGG







GCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DTX3. In some embodiments, the sequences from gene DTX3 comprise or consist of:











(SEQ ID NO: 282)



ATGGCAGCCTGTGGAGGCACCTGCAAGAACAAAGTGACTGTGTCC







AAGCCCGTGTGGGACTTCCTGAGCAAAGAGACCCCAGCCCGGCTG







GCCCGGCTTCGGGAGGAGCACCGTGTGTCCATCCTCATAGATGGC







GAGACTTCTGACATCTATGTTCTCCAGCTTTCCCCACAGGGTCCT







CCCCCGGCCCCTCCAAATGGGCTCTACCTAGCCCGGAAGGCTCTC







AAGGGGCTGCTAAAAGAGGCAGAGAAAGAGCTGAAGAAAGCTCAG







AGGCAGGGGGAGCTGATGGGCTGCCTGGCTCTGGGGGGTGGAGGG







GAGCACCCTGAGATGCACCGCGCAGGCCCACCCCCTCTCCGAGCA







GCCCCACTTCTGCCCCCAGGAGCTCGGGGGCTCCCCCCTCCTCCT







CCCCCCCTGCCCCCACCTCTTCCTCCTCGCCTTCGGGAGGAGGCA







GAAGAGCAGGAGAGCACCTGCCCCATCTGTCTGGGGGAGATCCAG







AATG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CORO1C. In some embodiments, the sequences from gene CORO1C comprise or consist of:











(SEQ ID NO: 283)



GTGTGTTTCCCTAATAGCGGGGCAGCTGGCGAGGGACGCAGTAAA







AAGGAGCGAGGTGCAGGTGGGGCCCCGCTGCGCCGAAATGACTGA







GGATGAGGTCAGACGTGGAGGGTGGGAATGCAGAAACCGAGCTCT







CCCGCCTGTCCCCACCGGCGCACGCTCCCCAGCTCTCCGTCTCTC







AAATGATTTGAATGTGTTGGGGGAAGTGGCAGGCTGCGGTGAGAG







CTGAGAAGAAAGGGGCTTCCTCTTGGAGGGAGGCGCTGGCCCTGG







CATCCTTTTTTGGGAGTGTTCAAAGGATTTTGAACGCTGGAAAAG







GAATCGTAGGCTTTACTGCATCACGATTTAGAAACACATCCTATA







TTTGGCCGTAATGTAGGCTGCCCTTGTTTTGTGGTGTCTAAAGTG







CTTGCTCTACATGTTGCAAACGTGGGATTCAGAGTGCGAAGCAAA







TTGCAAGTTGTTCATTTCCCCCGGCATGGTAAGATACAAAGGCTT







TTCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CORO1C. In some embodiments, the sequences from gene CORO1C comprise or consist of:











(SEQ ID NO: 284)



ACAGGAGCCGGCGGGGCGAGGGGCAGGGCTAGGTGGAGGGGACAG







TGCTGGGTGGGCGCGCGGGGCGCGGGTTCACGGGCTGGGTTGCAA







GGGGTTAGGGCCGGGGGCTGCTGTCGGGGGCTCGGGGAAAGGGAG







TTGGGGACCGAACCAGGTGGCGTGGGGAGCGTGAAGCAGGGTCCC







TGTGGGGAGGAGGCTTTCGAGGTCCGGAACGCTCCTGGCCGGGTG







CGGAGCCGCGGGCCGAGGTGGGACGAGGCTGCCCAGGGAAGGCTT







TTAGGGGTCAGGGATCGCCTGCTTGGGCGAGGTGCTGGGAGGGAA







GGGCTGCTTCGGGCGAGGCGTAGAGCCCGGGGGCTCCAGGCCTGG







GGACTGGGTTTGGGCCGCCCCAGGGAACGAGTGGGTGGGGGCGGG







TAGGGGCCGGGCTCACGTGGGGCCGGGGCGAGCTGGGCCCTGCGT







CGGGAGGGGCGAGGGCGGCCGCCTGGCCTGCGGGTTTCCTGGCTT







TGGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene VPS29. In some embodiments, the sequences from gene VPS29 comprise or consist of:











(SEQ ID NO: 285)



GTACTTTTATGTTGATTACCAGTGAGGGTGGAAACTTCATAGTGG







TTTTTAGTTTTACAGATGTTTTGTCCACTGAGATCTGCATTGAAA







CAATTAATATAGGTCATACAACAAATACCTGCTGAAACTAGTCTT







TATAAAGAAGAATCTTACTTAACAATTTTAAAGTGTGCTAATTTC







ATAACCTTGTGTTACTTTGAGATTACAAACTATGTCCCGATAACT







CTGTGTCCTGGTAACGGTTCCTTTTCTTTGCAATAATTTTAATTT







TTGTTGCAAGATAAATCAAGCTTCTTTTGGTAATCATTTTAATTT







CATACATTTTGTTTGTTTGATTAATTTTCATTTACAAAGTCTGCA







GTGGCAAAACTATTTTGTTTTATCCTATTAGCTATTTAATGGTTT







CTTTGATAATATTAAAGAATAGTCATGCCTTTAAAAGCAGTTAAA







GGGCATGTACATTTATGCATGCAATATCTGTAAAGGAAAACATTT







TGTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene VPS29. In some embodiments, the sequences from gene VPS29 comprise or consist of:











(SEQ ID NO: 286)



TTAAAGGACTCTATCATCTATGTCTATCCCGATTTGTCAAAGTGT







GGAATTTGGGCGGGAACATGTTTCAAAGTATGACACGTGTTATGT







AACACTATTTCCCCATAACTTTGTCATCAGCAGGAAACCAGAGGA







TTCTGTCCTAGTAAGGATCCCTACTAATTTGAAATGATTGTGTGG







TCATTCATACAGTTATATCTTTAGACTGCTAATAGTCTTGAGTCT







TGGAGATAATCCACAGTACTTTATAGAATTAGGTCATCAATCATT







ATAAAGTACCATGTCTTACTAATGTTCTTTCTGGTACATTCAGAT







TGAACAGCTCATTCATTATTAGTACCAAACATTTCAACCTGTTGT







AGACATATACCCTTTTATGAGTTTGGGGTGGTGGTTGTTGTTGTT







GTTCTTCTTCTTCTTTTAAATATAGAAATCTATTATTTTTACCTT







TTTCTCAAAGCAAGATTCCCATACTAACTATGTACTTCAATCCAT







ATCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CABP1. In some embodiments, the sequences from gene CABP1 comprise or consist of:











(SEQ ID NO: 287)



GGGTCGTCCTAGTCCCCCCGCACGGTATTGGGAGTGGAGGCAGCC







CCACACGGAGGCGCTGGGAGACTTTCCTCCCAGCAGTGTCCTGTC







ACTGCATTCCGGCCCCTGCGTTTGTAGGCAGTGATGTCACAGAGT







GCCTTCATGCTCCTCGGGTCTCCGGTTCTCCCCGGACCTCTGTAG







TCCTCATTGCCAAAGTTGTACCCCCTGGGGAGTGCACCCTGCCTG







CATTTCTGGAGGTTCGGGCGGTTCCGGGGCTCTGCAGGAGCCTCC







AGGCGTGATAAAGAGCGCTGCCGGCACTCCCGGGGTCTCGGCCTC







TTTGGGGCCCATTGCCAAGCCCCTTCCCTCCTCGTGCTCCCGCTC







CGCGTTGCAGCGTCCCCTTCGCCGCCAGGGGCCGCCCGGAAGCGG







GGCTCCCCCAGGGCGGGGCGGGGCAAGGGGCGGGGCGCGGCGGTA







CCTGGGCCGCCAGGGAGCCGGGGGTGGGCTCTCCGGGGACAGCCG







GCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CABP1. In some embodiments, the sequences from gene CABP1 comprise or consist of:











(SEQ ID NO: 288)



TAAGGGCCGCGCCTCCCGTCAGCGCTCCCGGGAAAGGCGCTCGGG







ACCCTGCCGGCCGCGGTTGCGCGTCCACAGCCTCCTCCCGCGGCC







CGTGGTCCCCCACGGATCACGCCTCGGCTCACCTCGTCCTCCCCG







GGCCGGCGCCCTTGCCGGCACTCCTCCTCCCCGTGCCTGATTCAG







CACCCCGTGCGCTGTCCACGCTCCGGGCCTCTTGGGGCAAGGCCC







TCCCCGCTAGCCTCCCTCATACCCGCCAGAACCCGCCAGTCCTGG







AAGAGAGCGACCTCTACGACCGTCCTGGAGATTTGCGGGGGTCAA







GGAGGGGCGCTCCGAGCAGGTGGCGCCAAACCCACTCCTCTGGCC







TCTGGCCAGTACCGCCAGGGAGGGACTCAGGAGAGCAAGCGGTTT







GGAGTCTGGGGGTCAAGAGAGGGGACCTCAGAGAACACAGAATTA







CTTGAGGGCGAGGGACACTGGCCACTGCCTATCATGTCCCTGAAG







GCCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CDK2AP1. In some embodiments, the sequences from gene CDK2AP1 comprise or consist of:











(SEQ ID NO: 289)



GTGAGCGCGGAGTGAACGAAGTTGGCGCAGCTCTGGGGCTGCTGG







GGCCCGCGCGCGGGCGTGGGGGCAGTGGGTCACGTGGACGCCCCA







TCTGGGAGCCCCCAAGGGCTGGCCCTGGAAGGCCCTCGATGCAGA







AGCCGGGAGGGGCTCCCCTCAAGCTTCCTGGGGCTGCGGCCGCAG







CCGGGCGCTCCCAGGAAACTCCTCCAGAAGTTGTCAGGCAGCTTC







GGGCTGAGCCCAGGGGACCGAGGGAGCCATGGGGAGTGGGCACAA







CATTGAGGGATTCGTAGGTGCCGGGCTTTGTGACCTCACTGGGGC







CCTTCTTCTCTGACACTTGGTCGGAAACAGACTTCCCAAAAGGCG







GTTGTGTTGCGGGGCGGGATGGGGGGAAGGACTCGACGGGGAGAG







GAGTGGCCCCGTTGGTCACCGGTGCCACGCTGGCTCTCCTGCTCG







AGTTTTGGGCCTTGTTTGCAAACGTTGACCTCGTAACCCCCCAAA







TCGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CDK2AP1. In some embodiments, the sequences from gene CDK2AP1 comprise or consist of:











(SEQ ID NO: 290)



ACTTGGCCGCGCACATGCCCGCCGCCGCCCTCAACGCCGGTGAGT







GAGCCGCGTGCGACGCGCCCCAAGCGCCGCCAAGTTGGGGGTCCG







CGCGCCCGGCCCCGGCCCGGGGATGCCCGGGGCGCCCGCGGAGGC







CGGGGGCGGGGGGCTCAGGGTCCCGGCCCGGGGCAGCGCTGCCGC







CGCCATGATTCCCGAGCCCGCGCCGCGAAAGTTCTCTGGTGCGCC







CCGCACGCCGAGGGCCTCGGCTGGGGGTGCGGGGCGGGCGGCCCG







GGGTGACCCCCGCGCCGGCGGCTGCGGATCGCAGGGAGCGCGGCG







GGCGAGGGCGAGGGCGGGGGAGGCTTGTTCTCGGCTCCTTTTTCC







TCCGGCTTCTGGGCGGCTCGGCGAGGGCCCCTGCCGGCGGGAGTC







GGGAGCGGCCCGGGCCGCGCCGGGAGCGGGGCGCGGGGCCCCGCC







GCCCTGGGGAGCGGGGTCGTGAAGCAGCCCGGGCGGGGGGCGCTG







CGGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene COMMD6. In some embodiments, the sequences from gene COMMD6 comprise or consist of:











(SEQ ID NO: 291)



GTGAGTTTGACTTTTTTAGATTCCACATATAAGTGAGATCACGCA







GTATTAGCCTTTCTGTGTCTGGCTTATTTCACTTTGCACAATGTC







CTCCAGGTTCATCCATGTTGTTGCAAATGGCAGGATTTCCTTCTT







TTTTATGGCTGTGTGATATTCCATTGTGTGTGTATGTATACACAT







ACATAGTATATACATATAGACATACATTATAATCATATTAAATAT







AAATATACACAAAAACATTTTCATTAGAGGCAACTCTTTTAAAGA







GAGTTTTTACATGGCTTAAAACTAACCACGAGATAGCCTGTTAAT







CACATTACATGACTGAGAGCTAAGGAACATTCTTTGAGCTTTTAA







TTTATATCTTTTTGATAACTAAAATTTTGAACATCAGTACATATT







TTTAAAGAAATAGTTACACTTTGCTTCATTCTGTGACAAATTTGC







ATATAAATTAAAACAATACAAATCCTCTTAAGTAGCAAAATAGAA







AATT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene COMMD6. In some embodiments, the sequences from gene COMMD6 comprise or consist of:











(SEQ ID NO: 292)



AAAGAGAATACACGTTTTGGTCTTTTTTGTAATTCACAGTGCTGG







CCTAATTGTTATTATTCTGTGCAGCATTTGTGCTGTCCTGATTCC







ACCTCTAGAAATTTTTTCCTATAGGAAAAAAAAAATCACAATTGG







ATTAACTTTTTTTCCCCAGCTTTATTGAGGTACAGTTGACAAAAT







TGTGTATTTTGAAGGTTTCACAATGTGATGATATGATAAAAGATA







TACATTGTGTAAAGATTACCACAGTCACTCTAATTAACACATCCA







CCCCCACACAGTTACCTTTTTTTTTTTTGGAGAGAGTGTGTGCAT







GTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGAGGGCAT







GTAAAATGTTTTTTAGCAAATTTCAAGTAAACAATAACAATATTA







CTGTAGTCACCATATTGTACATTAGATCTCAGACTTACTCATTTT







GTAACCAAAAGTTTGTACCTTTTTACTAACATTTCCCCATTGACC







CCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SLAIN1. In some embodiments, the sequences from gene SLAIN1 comprise or consist of:











(SEQ ID NO: 293)



GGTCATCACTGAGCCTCTTGAATCATTTCACATCATTTTATATAC







CAAAAATCCACTGAGATAATTGGTTGAAAAAGATAAAATTGCTCT







TTTTTACAAGTTCTTTTTGTTTGTTTTATTCTTTATAAAAGTGCT







TCCTGTTACAATTCTTTAGCTATGTACTGAAGAACAACTAGGATG







TGATAGCTTCCACTCCTGTCCTCCTCCTCCTCCACTGTAACCTTG







GGCACTAGTGGAGCATTATTGTTTTGTTAGCCTGCCTTTACAATA







ATTTTCCTCCCCTTTCTGAAAAGCTTCAATGCTAAACAACAATGT







AGAGCATTCTTTCTTGCAATAGACGCTAATTGTGTTCCTAGTTGG







GATCTTGCTGTCATAGCCACAATAACTGTGTCAATTGTGCAGGGA







GGCTGGGTCACAGTGGTTATTGTGCAGAGTGATGAATGCTGCTTA







CTTGTGATGTGCTGCCGCCCGCATCTGTGGGTGAGAACACGCAGT







TACT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SLAIN1. In some embodiments, the sequences from gene SLAIN1 comprise or consist of:











(SEQ ID NO: 294)



TAAGTTACGGGTTGGATAAGTGACATATACGACTGGTGTCAATTG







GCAACCAGGCTTCACAGTTTCATGAAATGTGTGGATTCTGAAAGT







GTTGTCATTGCGAATGTGGTTTCCTTGCCTAGTTCTTCCACATAC







TTAAGGAAAAGATATTTTTAATGTAATTGTTAGAATGTGTTGGTA







CGTAATGATTCTGAAGTAAAAGGAATTTTTTTTTTTTTTTTTTTA







AGTAAACTTGGTGTTTTGCAAGGATCTGAAGAATCCCAGTTTGGA







GCTGATTATTTTCTTGACTGTCCACATCCCACAGTTTTTTTTTTT







TCTCTCCATCCCACTGCCCATTTTAAAGCACTTTTCAGCTGTCTG







ATTGCTCATTAGCATTGCTCATTAAAAGTGGTCTGGGGGCATGCA







TTTCAAAGCAGTCCTTTTAATACTTGGTTTTTCAAGTTTTGTCAA







ATTCCCAGTGAATACAAACCAAGTTATTTCTTGAAGTTTAGCCTT







GTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene FARP1. In some embodiments, the sequences from gene FARP1 comprise or consist of:











(SEQ ID NO: 295)



ATCTTATTTCTAGATTTTTTTTTTTCCTAAGCAATCTCTTGAAAA







GGAATATCAGTGAGCAGATTTCTCTGGGGCCATTTGAACTTCATT







AAAATTTCAGAGAGGGCTTTTCAATGAGAATATATTCATGTAAGC







ACTGGAGAGTTTTGCATAATTTTAAAGTTGAACAGAATTGGGTAC







AATTAGTGATGAAAATACAGTCCATCCCAGATTCCTAGCTGATTG







CCATTTTGGTTTTCCCAGCAAACACCCTTTCTACCCCACTTCCTA







GCTTTATTGAGATATATCTGGTATATAAAAAATTGTACATAACTA







ATGTATACAATTTGGTGAGTTTGGATATAAGTATAGGCTGGTGTT







ACCATCACCCAGTCAAGGTAATAAACATATCCATCACTTCCAAAA







CTTTGCTGTGTCTCTTTATTGTTACTGTTTTTTAAAAAACAACAA







CAAAAAGCGCCTTTTCAAGGGAGTAGCATTTGCTCTCCTCTCTTC







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene FARP1. In some embodiments, the sequences from gene FARP1 comprise or consist of:











(SEQ ID NO: 296)



ACATGTTTGTCTTACCAGCTTATTGATAAGCACTTTGTGGGATGT







TTATGTGAGAACAGTTGATTTTAATGAGAAAACAGATTTTCAGCT







CCCATGTTCAGTGTTTTATGTGACAAAATCATACACTGCAGCAAC







TAAGTAATACGAAGTGAGTACTTTAATTGAAACAATTAGAGTGAC







ACAGGAGGCATTTTCTACTTGTGTAAACGCAAAGCTTAATTTTTA







AAAAGCCTCCCCCAGTTTTCCTTCCTAAAGGAAGTGGATGATCAA







GCCTGAGCTAGATAGAATAGCAGATGTGTATTTCATTATTAATAC







AAGAGGAATCTTTTTGGGTTAATAGAACTATTCTTTAGAAGAAGA







ACTTGTTTTGTGTTACAAAGGTGTTACATGGTTACTTACAGTAAC







AATTTCGAGCAGCACAGAAAATGCAGAAATGTATCCCATTCTTAG







GATTTTTGAGATAAATTCTTTTTTTTATTTGGAGAGGGAGTCTCA







CTCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene STK24. In some embodiments, the sequences from gene STK24 comprise or consist of:











(SEQ ID NO: 297)



GTAAGGGAGAGGGCATGAAAGCAGCGAGGATAGTAGCTGCTGAAA







ACGGGGTATGTTTCATTATAGCTAAAAAAGGATGCGCTGCTTCCC







CCGCATCCCCGAAAGCTGCTCATCCTAGCAGCTTGTGTTAGAGGA







GGTGGAGGTGCAGGTTCAAGGCCGCGTGTAGGGGGAGTCAGGTGG







GAGGTACCCACCCGGGCATTCTGTGGCATTCCCAAAAGTGACTGG







TGATACTCAGCCTGGGGTATTGATAAGCTTGAAAATAGGAAGAGG







AGGATGTTGTAGGGCTGCTCACGAGCTCCAAAAACAGTCTTCACT







TGAAAAAATTATCCTGGGCTTTGATTGTGGGTCATTGACTATCTT







GGTAAACCCAGGAGTCTTGGACTGTGTTTTTTAAAACCTGTAAAC







ACACAGCCAGGCTTCCTCCTAAGTATGACCGTCATAAGACATTTG







AAATGTAGTGCCGTGACTGAAAACAATTGAGCAATTGTTCAGGAA







AAAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene STK24. In some embodiments, the sequences from gene STK24 comprise or consist of:











(SEQ ID NO: 298)



TTCCGTGCACACATGTTGAGCTAGAGATGTCTGGGTAGAAAGAGA







GAATGCTTGGGAAGCAGCTGGTCTGGGGGGTTCCTGTTCAGAAGG







AAGGCCTGAGCTAGCGCTTTGAAAGCTGTCATGTACGTGAGTCTT







TAACACAGCCTTTCCTTCAAGGTGGCAATTGATCAGAATCCATTG







GGGTGTTTTAAAAACATGGATTATTCACCTCCTCTTCTGAACTCC







CATATATTTCTGCAAGAATGTCAGATGTCCTAGGTGGTTCTGTTC







GGGCAGGTTTGGGAAACAAGGGCCCCAGACCCTGAAACTACCGGG







CACCCCTGGGGAGAAGGAGTAGAGGCCCAGGCTGGGACCTAGAGT







AAGGGCCAGCCAAGGTGGAGTCTGCGTGAAGATCCTTCTGGAACT







AGAGGGACTGGAAGAAGCCAGCGGGAGTGGGGCTGTGGATGCTAA







AGGACTGGAAAGTTACCCAGAGGAGGGGGAGGGCGTGGTTGACAA







GGCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RNASE1. In some embodiments, the sequences from gene RNASE1 comprise or consist of:











(SEQ ID NO: 299)



GTCACTTTGTAGGTCACCACCTAGAGGGGAGGAAGACCTCGCTTT







GGAGAGTGGGAATAAAACGCTCGTGGAAAAGGGTACACGTGAGTG







TCTCATTACACGAAAAACCTCAGGAAGTAGGATGGACGTCTTTCT







GGCTGACCTGAAACAGAAAGTGTAAACCAGGCAAGCCATAGGTGG







GAGCTGGAGTTCTTTTTCTAAGAGGGCTCTTTTCCTTCTCTCTCC







TTTTCTCAATTAGGCTTTTCTGGGAAAGTGAGGCCACCATGGCTC







TGGAGAAGTCTCTTGTCCGGCTCCTTCTGCTTGTCCTGATACTGC







TGGTGCTGGGCTGGGTCCAGCCTTCCCTGGGCAAGGAATCCCGGG







CCAAGAAATTCCAGCGGCAGCATATGGACTCAGACAGTTCCCCCA







GCAGCAGCTCCACCTACTGTAACCAAATGATGAGGCGCCGGAATA







TGACACAGGGGCGGTGCAAACCAGTGAACACCTTTGTGCACGAGC







CCCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RNASE1. In some embodiments, the sequences from gene RNASE1 comprise or consist of:











(SEQ ID NO: 300)



AAGCCAAACCCCTTTGTAATCTGTATAAGGTCCACACCCCGGGAG







CTGAGTGATTGCAGAAACTGGCCTTCCATCTCTCTCAGACACCAA







GCTGCAGATCCAGGTAAGCAGCCCAAGAGGTGTTCTCAGGTGCCG







CTTCTGCTTCAGGGTTGCAGAAGAGGGAGCTAGGGCCAGTCCACA







GCACTGAGAAAGATTGGGTGATGCTAGCGCTGAAAAAATGTGGCC







CAGGCAAACATCAGCTGGGGGCAGAGGGGTCAGGGTGGAACGTGC







AGGCTGCAGAGCATCCATGGGACTGGAGAAGAAGGAGCCTCCAAA







TTGGGGGAGGCCAGCGTTTCTCTCTGGAATACAATGGAATCTTTT







ATGATGCCAGTGTCAGGCACTGGCCTGAGTTCCCTTATGCTCCAA







GACAACAAGGCTCTGTCACCCATGGCCCTGGATGTCGGGGATACA







ATTTGGTTCATGATTTATTCAAACTGATGTTTCGATGAGCCATTT







GAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NDRG2. In some embodiments, the sequences from gene NDRG2 comprise or consist of:











(SEQ ID NO: 301)



GTCGTGGATGGGGCGGTGCTGAGGGCAGCCGAGGGATCCCTCTCA







GGGCTGAGGGATCCCCGCACTCCCCACACCGCCCCAATGCGGGGA







GGGGGTGGTGGAATGTTTTGGTGAGCCGAGCAGAGCCTGGAAGCG







TCCAGCCGCCCGCCCCTCGCCCCCCGGCCCCAGCACAACCCTTTC







CTGAGCGGCAGTCTGGGCGGGGTGAAAGGGGGGGCGTGTTTACTG







GGGGTCTGGGGCGGGTCTGCGGGGAGCCTAGCGCCCGTGGGCGGG







CTGGGGCGGGCCGGGGGCGGGTCTGCGGGAAGCCCGAGCCGGGGC







GGGCCCGAGTCAAAGGCAAGTGAAGGTGGAAGCGGCCGCGGCGGC







AGCAGGTAGGGGGAGGGGCAGGCGAGGGGCCGTAGGGCCTGGAAG







GCGCCAGCCGGGCCGGCGGGCGGTGTGATTGATCCGCGTCCCCTG







GAGCTGGAGGCTCGGGGGAAAGGGCCAGCACGGAGCGGGCGCTCG







GTTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NDRG2. In some embodiments, the sequences from gene NDRG2 comprise or consist of:











(SEQ ID NO: 302)



GTGTGTGTGTAAAGTCTACAGTGGCAAACCCACTCGGGCACCGAG







AGGGACGCGGCAGACCCTGAGACCATCCCCCGGGCATTGCGATTC







AGCGGGCCTGAGCTGGTCTCCTATTCTGGGCCGGACTGGGAGGGG







TTAGCGGCGAAGCCACAGGGTCTCTTGGGTCCCAGCCCAGGACAC







TGCGTCCTCCCCAAGCCCCTACTCCACCCCCGCAGGGTTAACTCC







GCCCCTGCGCCTGTTTACCCAGGAGTCAGAGCCTCTGGGGGTCCC







CGGCCCCTCCCCCGCGCCCCTTGAGGCATTGACCCCAGAGTCCCT







GTTGGCCATCCTCTCCCCACCCCCCGCCTGCCCGCGATCGCCCTC







TATTTATAGCGGCCCCCCGCATTCCCCGCCCCCACCTCCCCGCCT







TGTTGTCCAACTTCTCCCGGAGCAGCCGGAGAGCAGGCGTCGGGA







CGCAGCAAAGAGAGGAGAGGTACCAGGACTCTGGGCACGAGGGAG







TCGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PSME1. In some embodiments, the sequences from gene PSME1 comprise or consist of:











(SEQ ID NO: 303)



CAGATACCTCGGATTGAGGATGGTAACAATTTTGGAGTGGCTGTC







CAGGTGAGAGCGCTGCCCCACTTCCCTGCTCTTTTCTAGTCCATG







CTTCCTTCCACTTTCCCCCTTGCTTTTTTTCCCTAGGAGAAGGTG







TTTGAGCTGATGACCAGCCTCCACACCAAGCTAGAAGGCTTCCAC







ACTCAAATCTCTAAGTGAGTGACCACCCATGTGCACACTGTTTTT







GTTTTGGGAGACCTCCTTCTTCTACTCCATACACACTTCTCCTTC







CACAGGTATTTCTCTGAGCGTGGTGATGCAGTGACTAAAGCAGCC







AAGCAGCCCCATGTGGTAGGTGAGGCCCAGGTCAGGGTGCATGGG







GGAAGGACACATGTAAGGTCAGGCCTGACCCGAGCTTCCCACAGG







GTGATTATCGGCAGCTGGTGCACGAGCTGGATGAGGCAGAGTACC







GGGACATCCGGCTGATGGTCATGGAGATCCGCAATGCTTATGTGA







GGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PSME1. In some embodiments, the sequences from gene PSME1 comprise or consist of:











(SEQ ID NO: 304)



CTGTGTTATATGACATCATCCTGAAGAACTTCGAGAAGCTCAAGA







AGCCCAGGGGAGAAACAAAGGGAATGATCTATTGAGAGCCCTCTC







TCCCATTCTGTGATGAGTACAGCAGAGACCTTCCTGCTTTTTACT







GGGGACTCCAGATTTTCCCCAAACTTGCTTCTGTTGAGATTTTTC







CCTCACCTTGCCTCTCAGGCACAATAAATATAGTTATACCACTGC







CCATCAGCCCAAGTCTCTTTATTGGAACCGGGCCCCGCTGGCCCT







GGCTCAGGCATTTCCATTTCCATTAGTGGGACCAACCCACTGGGT







GATTTGAGTGTTGAGCCGCTGGTTGGTCCAGTCCTGCCGGATGTC







ATCAAGGTGGCAGTCAAAGTCCACAAGGTGCTGGTGGGCCCGATC







TTCCAGTAGAGCTCCCACCATCTGCCGTGACTCTTCCCAGTCCCT







CCACATCACTCTGAAATAGTAACCCCCATGGAGGTCAAACAGCAA







GTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene REC8. In some embodiments, the sequences from gene REC8 comprise or consist of:











(SEQ ID NO: 305)



CCAGAGTCCTGCCTTTTCTGTCTCCATTTCCCTATTCTCTGTCCC







TGGCACTTGAGCACCCAGTGGCTTCCTTGAACCTGGCCCTGTGGC







ATGCCTCTGGGATCCACTCTCCTGGATCCACTTGCCTTTTTCAGA







AATATTATTGAATCCCCTCCCCTTGCTCTTCCTCTCTGGACAGGG







TGAACGGGAGCTCCCAGAGGTCAGCCGCCGAGAACTGGACCTGCT







GATCGCAGAGGAAGAAGCTATCTTGTTAGAAAGTAGGTGTCTCCG







GCAGCGTAGGGCCCGCCTGGAGCTGGATAGTCAGACCCCTGGCGT







GGGCATTCTGGGACTGGGCAATGCTCCCATTTCTCTTTTTCTGTC







CTCTGAACTCTGATTCTCTCTCCACAGTCCCGCGGCTCCCACCTC







CAGCTCCTGCAGAGTAAGGGCAAGAACTCCTAGACCAGGTAGGGT







GTCAGTGCTGGGAAGGGTCTCCTCATTTCTCTTGCCCATTTCCCC







TCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene REC8. In some embodiments, the sequences from gene REC8 comprise or consist of:











(SEQ ID NO: 306)



TGAGTGCCCACCATGCCCCAGGGGCTTTTCTGGGAGTACCTGGAT







ACTGCTGCAGACAAGGGCTTTATATCCCAACTTGCTAAAGGGAGG







ACCCTGCAGTTTCTTGCCCTGGCATCTGCAAGGGGTAAGGGGCTT







ATGGGACAGAGCCCCTTGGGTGTTGTTGCAGAGGTGACCCCCCCG







GAGGAGCTGCGTCTGCCAGCCCCACCCAGCCCAGAGGTGAGTAGC







CTCCCTTCTAATCCTCCTCCTCCTCCTCTTTGCCCTCCCCCACAA







GGACTGCCTCCCCAAGCCCAGGGCCACTGCTAGCTTACAGGGGTC







CTTAATGCAGATGATAAAAGATGCCCCTTTCTACGCCCCATCGTA







TCTGGCCTACGCTTCCCAGATGGGTACCCTTATGCAAGGTATGAG







CTGCTCAAGTGCATGGAGACCCCGCACAAGCCCTCTCCAGGTTCC







TCTAGGGGCGGGTGTGGGGTCCTGTTAGCAGTCCACCAGAATGAC







AGGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SDR39U1. In some embodiments, the sequences from gene SDR39U1 comprise or consist of:











(SEQ ID NO: 307)



GTGGCGGGACAGGCTTCATTGGGACAGCCCTAACCCAGCTGCTGA







ATGCCAGAGGCCACGAAGTGACGTTGGTCTCCCGAAAGCCCGGGC







CCGGCCGGATCACGTGGGTAAGTCCATCCTCTGGAAGCGGGTGGG







AGGGCAGAGTTGGGCGGCGCAGGGCGGGGCAGGGGCACTGTGTGC







TTTCTCCGACAGGATGAGCTCGCTGCATCGGGGCTGCCGAGCTGC







GATGCCGCCGTCAACCTGGCCGGAGAGAACATCCTCAACCCTCTC







CGAAGGTCAGCCCGGGCCCTAAAGCTGATACCCACTAGAGCACAG







GGAGGACAGTGCCCCACTGATGAGAACCTGTGAGCTATGGGTAAT







GGAGCTCCCAGATTCCGAACTAAACCGATATCCCAGACTACTCAT







TCTGCTCCACTCCTCCACCCCCACCTGCTCCTCCACCCAGTCAGC







CAATTGGCTTAAGTCCTCACGTATAACTCAGGATGCCCAGGAAAT







GAAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SDR39U1. In some embodiments, the sequences from gene SDR39U1 comprise or consist of:











(SEQ ID NO: 308)



CTTGCTATTGAAACACCCAGCCTATATTTACAAGTTTTTCCGGGT







CGCCGAGAAGGGTCACGCACAGGATTGCGACCTCGATTGGAACGC







GCTTCCCGAAAGGGGCATGCGCAGAGAGGTCGTAGTCGCGTCTGA







GGTGAGGTAAACTCTAAACTTTACGCAGGCGCGTTAGGTCCTAGT







CGCTATGCGTGTGCTTGTGGGTGAGGGAGGGCAGAAAGGGAGAGT







GCTGGGCGGGCTTAGTCGGAGATTGAGGACTGGGAATCCGCTTCC







GGGAGGGCACTGTCTAGTGCACAGGCAACCTGGCCTTGGCCTCCT







AGCCCGAGAAGCCGAATCTCCCTAATCCCTGTGACCTGTGTCACC







TCTGCATCGCGAGGAGGGGGATAAGTGGGGAGAAGCCTGGTGTCA







GATGGGATGGCGCCGGAAGAGGGTGCCACAGCGGGGACGGAAGGC







GCCCCCACCCCAACTCCACGGGAATATAAACAATTTGTATTTTCC







GATC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RTN1. In some embodiments, the sequences from gene RTN1 comprise or consist of:











(SEQ ID NO: 309)



GTATAGTTGCTTAAGCTGTTCACTTCACAGTCTTGACTGTTATTT







TCCCTAAAGGTGTAATACTGTAAACTGCTACATCAAGAAGAATAA







AAAGCAAGTGCTAATTATATTCTAGAGTCAGAAGACCTCCAGTTG







TATGGCACCATGAACCAGTCTAATCCCTTCATTGTACCAATGATG







AAAATGTGGCTGAGACATTTGTCCAGGGTTGCCCAGTGAGTCACT







GGCAGAGCTGGAATCAAACCCAGGTCACATAGCTTCTAGCTCTAT







AGTCTTTACAACACACCAGATTGTCTCCTTAAACCATTTTTAGTT







CCTCCGACAATTTCCCAAAATTAATATTAAGATAAAAAATCTAGA







TAGGGAATGTGAAAACCATATATACTTTTGCATTGAAGGAAACTC







TAGATTCTACTAATATTAATAGTAAGCCCTCCAAGTGTGCTTATG







TAGTTACAGCTCTGAGGCTAGAGGTATGCCCAGCCTCTCACTTGA







TGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RTN1. In some embodiments, the sequences from gene RTN1 comprise or consist of:











(SEQ ID NO: 310)



TATGGCCCAGGTCACTTGAGTTTTCAATGCTCTTTCTATAAACCT







GGAAGTAAATTCTAGAATTTAAACATGAATTCTTAGAGATCTGAT







GAGGAACAGTTGGCAGCTAGGATTTTGAGCTATCTCAACTACTTA







AAAAAAGATAATTTATCTCCATGAAGTCATGTGTGGAGAATTCAG







CAAATGAGCTGATTTGGCACTAATTTTTTTTCTCTGGTCCCAGCT







GGTTTCTCTGAATTTTCAGCCCACCCATGCTGTGTCTTAGGTAGC







CAGAAACAGTCACAATGCTCCACTGAGAACCATGCTTTCTGTGTT







CTCAGTGCATGGAAGCAGGTGTGTACGGTGCCCTGTGGCCCTCAC







TGGCTGGCATAACCCCCCAGAGATGACTCCTGCTCTCACCCTCAG







AGTGAGAGTAGGAACGCTTAGCTGTTACTGGGGTTCGGCAGCCTG







TCTCATGATTCCCTCTGACGGAATTTTCTGTGGGAATGGTTTGTT







GACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SLIRP. In some embodiments, the sequences from gene SLIRP comprise or consist of:











(SEQ ID NO: 311)



ATAGATATTATAAAAAGTAAATCAGTGAAGTACAAGCACTGTAGT







TCAGAAGAGAAACAGATCGTGTAGTTTTTAGTCTTTAAGATTATA







TACTCTAAATCTTTGAAGTCTCACAGGGCTGGTACATTGTCTTGT







ACTGTGATAGGAGCCCAATAAAGTAATACTCAGTGGATGTACTTG







GTTTGGGTCTTGAAGGATAGTTATAGTGTTACAAGTTAGCCAGGG







GGAGATAGGATTACCAACATATGGAGGGGAGAGAACAACATATGC







AAAGAGGGTAACCCGCAGGACAGATAAAGATAATTCAATTAGTTG







TACTAGAGAGTAACTAGGGGCTAGCAGTGGGAAATAAGGCAGGAA







GGGTAGGTGAGGGCAACTCAACACATTTTACTAAATTGTGAAAGA







CCAGTAAGTTGGGATTTTATTTGGAAATCTTCAGTATACCGGGAA







ACACACTGGATTGTAATTTGTCTTTTTCTTCATTGTGATCTGCTC







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SLIRP. In some embodiments, the sequences from gene SLIRP comprise or consist of:











(SEQ ID NO: 312)



TACATTATGGCTTTATAGTCAGTACCATAGCTGCTTCCTAAATCT







TAAGCCTTTAAGGAAATGCAAAGTAGTTCCTGTCCACAAGAAATC







TGGTGACCCTGTCTTTCTACAGTCTAAGATAGAGAATCCAAAATA







TAGTAACTACTTTTAATGTTTTCTTCTGCCACACAGGTCAGCTGA







AAGAACACTTTGCACAGTTCGGCCATGTCAGAAGGTGCATTTTAC







CTTTTGTAAGTATTAAGGAAAAGTAGGTGGGAGGTGGGGATGGCT







AATGGGTACAAAAAAAATAGAATGAATAAGACCTATTTGATAGTA







CAACAGGGTGACTATGGTCAATAATAATTGTACATTTTAAAATAA







CTCAGAGTGTAATTGGATTATTTGTAACTCAAAGGATAAATGCCT







GAGGGGATGGATACCCCATAAGTCAAAGGATAAATGCTTGAGGGG







ATAGATACCCCATGCTCCATGATGTGCTTAATTTACATTGCATGC







CTAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene HSP90AA1. In some embodiments, the sequences from gene HSP90AA1 comprise or consist of:











(SEQ ID NO: 313)



GTAAGTGGACTAAGGTTTAAAATTACTATCCTAAACATTTCTAGC







TAATGTTAACCATCCATCCAACCAACCGGTGGTTGGAGTCACAGA







AAGCTAAAACAATGTCACTCTCTTTATTGTTTATTCCCTGCCTAC







CCATCCACTGCATTGACATCTACTGAGGGTCCACTACAGGCCCAG







GCAATGTCCAAGAGAGTCCCAGGTCCCTGCCTGGAGTGCATGTGA







CAATCATCATAATACAGGGCTATAGCGCATATATGACAAAGTGCT







GTGGGAGAAAAGAGAAAGACAATCCAATCCCTTGGGCTCCCAGAA







GAGGTGGCTTGTGAGCTGCGCCTTGAGCACCCACTCACGGAGGAC







AGCATAAGCAAAAGTGGGGAGGAATGTGGGCAGGAGGGGCGTGAA







GAAGCGGGCAGTGGGGTTTGGAGTGACTGAACCCTAGGAGGGTGG







TGGTGGCAAGAGATGATGGGGGAGTGTTGGAGGGGCCTGGGCAGG







CTAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene HSP90AA1. In some embodiments, the sequences from gene HSP90AA1 comprise or consist of:











(SEQ ID NO: 314)



TTGATGCATTTAGCTTACTTTCCTAAGGGGCTTTCAAGGGGGACA







CTTCCCCTGGATTCAATTTTCCCCTTAGCCACTAACTTTGAAACC







CAACCAGAATTCCAGACCCTTCAGTGCAGCTCTCCTGCAGTTGGT







TGAGTCATTAGGATAAGTCTGAATGGAGCCTGGAATAATAACAAC







TAAGTTCTAGTTTTTGCTGCAGGCCCTCTAGCCCAGTCCAAGAGC







ACCTGGCCACCCAGAGAAGCACTGCTGGAGTGGGGGATCAGGCCA







TACAGCATCCACAGAGAAGCCCAGCTCCTTACTGGGACCCTGGTC







CATTTCCCAAGTTGAACTTCCTGCAGGAAGAGAACATATCACATA







TTTTAATCCATTTGACAGTCACAGAGGAAGTCTAAGGAAGCCAGG







GCATGCCTGCTTGGGGCTGTCCAAGTTCATCCTCTGCCGCCCTAT







CTCTGTTATGTTTGGTTTGGAAGTCCAGAAGATTGTGTTTATGTT







CCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CRIP2. In some embodiments, the sequences from gene CRIP2 comprise or consist of:











(SEQ ID NO: 315)



GTCCCAGCTTCCTTGAGTGGAGTGGTTCTGGTCAGAGGGCTCTAA







GCTGGTGCAGCCTTGTGGTTGGGCTGGTGTCCTGGAGGCTGGCCC







TGGCCATGCCCAGAGGGTTGTACCAGGGCCACGGAGAGCCAGTAT







TCACAGCCCTCTACCTAGTAGGCCACCTGGGGGATCCCATCTCAC







TGCCAGGAGCAGCACCCTTGGGCCTCTCAGAGTGGGCTTCGCCCT







AGCCAGATGCCCCTCCCCGATATCTGCCAGACAAAGGGGGCTGGG







CCACTTGCTGAGAAAGGGAGCTGGGAGAGCCACTGGTCCTCGGCC







ACCTGAGGGGCTTGGCCAGATTCTGCCGGGCAGGGTGGGCCCAGC







CTGCATGCTCCCTCCCCCCAGGGCCTGCAGCTTGTCCCCTTCCCT







TCAGAATTCCGGCTCTGCTGGGCCCTTCCTGCCCCTCCCCACCTC







TTCCTGCCTGGCTCCCTAGGGAGGCCCCTGCTTCCATCCACTGGG







AAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CRIP2. In some embodiments, the sequences from gene CRIP2 comprise or consist of:











(SEQ ID NO: 316)



TGTGTGAGTGTAAGAGAAGTGTTTGAGGGAATGACAGGTGGGGGA







GATGCATATGTGTGGGGGGAGGCAGGTGTGTGGGGGGAAGCGGGT







GTGTGTCTGAGGAAGCGGGTGTGTGTGGGGGGAAGCGGGTGTGTG







GAGGCGGGCGTGTGGGGGAGGCGGGTGTGTGTGTGAGAGGAAGGT







GTGTGGGAGGAGGCAGATTTGTGGGGGGAGGCGGGCGTGTGGGGG







AGGCGGGTGTGAGGCGGGTGTCCGGGAGGCAGGTGTTGGAGCGCG







GGCAGGTGTTGGAGCGCGGGCAGGTGTTGGAGCGCGGGCAGGTGT







TGGAGCGCGGGCAGGTGTTGGAGCGCGGGCAGGCTCGAGCACAGG







CCCCTTCACCGCAGGCACTGAGGGGACTAACAGGGCAGCCCCGGG







CCCCTTCTCAAAGGCGGCTCTAGCGGGGTTCCAGGGCTGGGGGCG







CTGAGACCCAGAGGGAGAACAGGGCCTGGGGGCGACGGGCTCCTC







CGGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene APBA2. In some embodiments, the sequences from gene APBA2 comprise or consist of:











(SEQ ID NO: 317)



AGGGAGAGGATGGGATAAGCCTGAAGGGGAAGGTACCCAAGATAG







TCTTTGAGGGGTCATGGATTTAGATTGGGAAGAGTTATTGTCCCT







CCTCATTGGTCCTCATTATGTCTTCTTAATGAAAGCTGCTCATTT







TCTTCGTGAGAGCAAACAACCTGATCTTCTGACCATTTGTCTTGG







TCACCTGGGCTACCCAAACTGAGTGAGAAGTCAGGAATGAGCCGG







TAGGTTCCTGGGGAGAGGCGTGTGTCCTCGTGAGTTGCCGGTGCC







TCCCTTGACTGCTTTTCCCGGAGCTCTGTGTGCCCTCGTTCTCCT







TGGTGTGGATGCGTGTTGTTCTGATGATGAGTTAGCGGGGGTGGG







GATGGCGCACTGTTTGAACAGATGTGCCCTCACATGGCTGCGGGA







GGACGTGGTGGGAGGTGCTGTGGTGAAGTGGCCCCACACATGGCT







GGGCTTTGTGGGGTTTTGACTCTGTCCTTCCCAATGTTCCTCCCC







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene APBA2. In some embodiments, the sequences from gene APBA2 comprise or consist of:











(SEQ ID NO: 318)



TAGGACCCTGGCTGTCCTGGGGAAGGGAGCAGAGGGGCCCGAGAG







CAAGGGACCTCAGGGTACAGGCCTTGCAGATGCTGAAGCGAGGCG







GTGGGGGGTGCTGGGTGCCTCACAGTTCTAATGGTGGCTGAGCTC







TTCATTGGTCCAGTTGGGAGACATGTTGCGTGGATGCTCCGGCCA







CTCTTAAGCTCACCGCTCAGACTCAGGACTAAAGCCGGTTGAGGG







CTGAGTGGCAACTCGTGTCTCGCAGAAGACACCCCTCCTCCCACC







CTAGAAGAGAGGTTCGATTTCTTGCCCACTGCCCTGGTCTGCTCA







TGGTGGGGCAGTATGTGTGGGAGAGCCCCTCAATCCTCAGGGGTG







TACTCAGACCAGCAGGGCAGTGGCAAAGACGTGGGGGTGCTGGTG







CTCACCCACTGCTGCGGCCAGTGTTGGGTGGCCTGGTGGCTGGGG







TCGGGGCCAGAGTGCATTTCACAATGGACGGGTCTCTGAAAGCCC







GGGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SCG5. In some embodiments, the sequences from gene SCG5 comprise or consist of:











(SEQ ID NO: 319)



AATGTTTCCCTGCCACCACTCCCAACCTTCCTGAGCTTCTTGATT







TGTGTCTTCCTGACTCCCTCCTGTTAGACTTTTATTCCCAGAGAC







TGGGAAAACCCAAGATGCCACTATCTCTTTCTCAAAATGTAAGCA







AGTTTGGTTTATTTGTTTTAGTAAGAGAGGGAGAGAGTGATGAAG







TTAGGAGTAATGCAGAACTTTCAGGGAGCTACAAGGAGGATAAAA







ATTATGAGTGAGAACCGCCATTCCAGCCTAGCTTTCTCAAGAATG







CAAACAGAGGGAATTGGATACCTGCAACTGTTTTGTTTAATACCT







TTCTGCAATGATGCTCTGCCTAGCATGGAAACTTAAGACAAAAGC







AACCTCCTAAGGATTCTTTGTTACAACCCCTTTCTGGGTGGTCCT







CGAACCACAACCTGGAGTGGTTGCATCATTATAATTGTATTATCA







CAATTGCCGATTGTAGCCTATCAGTATACATTTGGTCTTTTATCT







GCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SCG5. In some embodiments, the sequences from gene SCG5 comprise or consist of:











(SEQ ID NO: 320)



TATTTACTGTGTTCTGATGGTTTGAAGTTTCCGTTAGCATTTTAA







ATAATATATTTGCACACTTCTCACAACAACCTTATAAGTAGATGC







CTTTATTATCCTATTTTTTTCAGAGGAGGAAGCTAATTTTTAAGA







GACTCTACTTTCTCATGGTTTTCCCTTTCTTCTTCTACACAGTGT







CTACATACTTACATATACACACCCACATGAGACTATAAGTCCTGT







GAGAGCAGGGACCTTATCTATCTAATTCATAGCTGTAGCCCTAGC







ACCTAGCACAATGTCTGGTACATAATAGGTGCTCAATTAATGTTT







GTTGAATGAATGAAGGAGCATCTAGTGTTATCACTAAAGAAAGAT







TAGAACCCAGACATGATGTTTTCTCCTCACTGCTGATCTTCAGGA







AATTGTGTCATCAGATAGCTGCCTTTTGAGTCAATGCCAGCCAGT







ACTTGTAAAAGAGCTTCAAAATGTAGAACCTACAGGATCTGACTT







GATT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SRP14. In some embodiments, the sequences from gene SRP14 comprise or consist of:











(SEQ ID NO: 321)



GTGAGAAACAAAGCTTTTTTTTTTTTTTTCCCCAAAAGGAGCCTA







CTGCCTTTCACCTCTTGGTGCAATTCTCATGTGGAAAAAGATGCA







GAGCTCCTTTTCTTGTTCTCTAGGCAGAGGAAAGTAGAGTAGAAA







AATAACCATTCGTGTATTATGAACCCTGAAGTTGGGGAGAAGTAT







TTTAAAGATAAGGATATTGTCTTCCGGAGGCTTTTGAGATACTAT







CTGGGGTGGAGAAGAGGATGCTTATATGGATAATAGGTATGACCT







CTTTTTTCCTTCTTTTTCAGGTGAGCTCCAAGGAAGTGAATAAGT







TTCAGATGGTGAGTTTTGGGTGTTCCTTATGTTTCCCTTCTCAGG







GCAAGAGTCAGTATGATAGTGAACACTGGGTAAGATTGATACTTG







GGTTCACTGCTCTCCGGAGATAAGTTTTACCAGTATGTTTCAAGT







AAAAGCTGCCAGGGTGTGAATTTCAATGCTAATACCGTCTTCCTT







TCTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SRP14. In some embodiments, the sequences from gene SRP14 comprise or consist of:











(SEQ ID NO: 322)



CTAGTCTTTCAGTGGAGGTTGAATAAGGCAAAGGAAGAGGCTCCT







TGAATTGAGTGAATACTACTTTATTCTGCTGTGGCAGAAATCACA







TTAGTGTAGGATACTGACTGCATGAAAATGTAGACTATACTTAGT







TGGAAGTCTTGAGTTATCTTGAGAGGACTTCTACAGTAAAACAAG







CTTATGTGGTTTTACAGATTAAGTTAGATATCATGAAATAACAAA







AACCTAAGGACAAGTTATCTTGAGAGGACTTCTACAGTAAAACAA







GCTTATGTGGTTTTACAGATTAAGTTAGATATCATGAAATAACAA







AAACCTAAGGAGTGTTCTTCAGAGGTAGAATTTATTTTGTGGTAG







CTCGCATTGTGCTTAAATATGCTTGAATTAATGGAAGGCAGGTTT







AAAAATAAGATGGGGAGGGAGTAGTTTAAATATATCTTCTATTAA







TGTATAAAAAATTAATACTGAGAAGTCCTCTGACTTTTCTCAGAA







CCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SHF. In some embodiments, the sequences from gene SHF comprise or consist of:











(SEQ ID NO: 323)



GTGGGTCCTGGGGGGCTTCTGGCCAGGGTGACATGTCCTCACCCC







TCCAGGCCCTGTGGGTTGGGTTGGAGGAGCTGAGAGGTGGGGCAG







GATTTGGAGACCCCAGGAAGAATCTCATTTGGGAAGAAGGAAGAG







ACAAAATGCACGTTCCTCCCTTCCCCACAGCTGTGGGATGGTGTC







TTCTACAGGGAGGCCCCTTTCTAATGTGTTCCTCACCATGCTACA







AAAATCCACCATTTTAAATTCACTTTGGGGAGAGCTTTGCTTGAC







AAGCTCCTTCAGCAATGCAAATACTCCTGAGCTGGGGGTGGGACA







GACACAGCAAGTGTGGAACTCCTTGGCGCAGAGGAGCAGATCTGA







CCTTGCATCTGGTGACTGAAAGAGAGTAAGGGCTTCAAAGTGCTA







CAAGGAGAGAAAAAGAGCCCAAATGAGGAGAAAGAACGCACTTAA







TTCTTCCTCTATGTTCAGTTCTGCCCAGAATGGGCACCCAAGGGT







GTGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SHF. In some embodiments, the sequences from gene SHF comprise or consist of:











(SEQ ID NO: 324)



CCTCAAGTCTGAGTAATCCTGACAACCTGAGGCGAGGCTGGCTCT







TTCTTGCTGCCTGCGAGTTCCCAGGCTGTGGGGAAAAGGAGAAAG







GGAGGCAAGGCTGTGGGTGCCAGGAGGCTATGGGAACCCAGATGT







GGTGTTGGGAGGTGCTGGTCACTCTTAGTCCTTCATTTCCTTTGT







TCCTGGTACCTTTGGGCCCTGGAGGCTTGTGAGAGGTCAAATGAA







GGGGACCCACGGGCTGACAGTGCAAGCCCCTGCATCCTGCTGCTT







GCCACCCAAGCCTCCCTCCCTCTGACCAGGCCAACCTGGTGTGGT







CAACGTTGGCTCTGCCCAGCCCTTGAGTGCCTCCCCAAAAGCTGA







CCTGCCTCTGCCTCTGCCTCTGCCCCTCCTCATTTCTAGGCTGTC







CTGTGTCCTGGCTCTGGGTTTTGGTGTCTCAACCTCTACCTCTTT







CTGTCTCTGTCACTGTCTGTCTCTCTTCTCACTTCTGCTGCTGGC







TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DUT. In some embodiments, the sequences from gene DUT comprise or consist of:











(SEQ ID NO: 325)



TCCTAGCCAAGGGGTTCCCACTGCGTTTCTGTTGGGTTATCCCAA







ACCTGTGATAGGATAGTAGCGCCCTCCCTGCCTCCGGTCGAATAA







ACACTACAACGTCTGACACTCGGTGCTGAAAGGAAATCGGGCGCG







GTGTCTTGCCCACAGCTTGGGGAGCGGCCCCGGGCGCAATAACTG







TCACCGGCGCCGAGATGCGGTTCCGGCGCTTAGGGCGCCGCTAAA







CTCAGAGCCCGGGAGTCATGGCTGCGGGCGGTGCCGCCCCAGGTA







AATCAGTCCAGGAGCAGGGCCCGGGCCTGGCGTACACTCTCGGAA







AAATGGGGGCCAGAGCAAACAAGAAGAGCGAAAGCAAGAGGGCTA







GGCAGCCAGAGGCGGCAGCAAGACTCAAGACGCCAACGGCGCCGT







CTTCCTGGGGCCCCAGGGCCTGCGCCATCCCTGGGCTGCCGGGGC







ACCGCCTCTCCACGCCCCTCGTCCGGCGGCGGCTGCGACTGCTTC







CGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DUT. In some embodiments, the sequences from gene DUT comprise or consist of:











(SEQ ID NO: 326)



TGCCGAGGACCCAACCAGCCCAAACTCTGGGGGAAATGACTCCCC







TCTGCCCTCGCCCCGCGCTCTGCTACCATTTCCTTACGTCTCTGC







TTCGCTCAGCGATGCAAAACGCGCGAGGCGCACGGCAGAGGGCCG







AAGCCGCGGTACTCTCCGGGCCAGGCCCGCCCCTCGGCCGCGCCG







CGCAGCACGGGATTCCCCGGCCGCTGTCCAGCGCTGGCCGCCTGA







GCCAAGGCTGCCGCGGAGCCAGTACAGTCGGGGCCGCTGGCTGGA







AGGGCGAGCTTCCTAAGGCGGGGGGAAGCCCGGCGCCGGGGCCGG







GTAGGAAAGGCGGGGGAGGGGCTCCGGCCGTCTGGAAGGAATCCA







CGCGGCTTGAGGCTGTGGGGGAAGTAGGGTGGCGAGCGGTCCTTC







TGCGCGCGGGGGGCGGGGGGGGTGGGGTGGTCCATTAGGGTCCCC







TGGCGAGGGGGCGGCTTTCTAGTGTGTGAGGGCGACGCCCTAGAA







GCTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SPG21. In some embodiments, the sequences from gene SPG21 comprise or consist of:











(SEQ ID NO: 327)



GTGAGTCTCCAGCGGGAGGTGGGGGCGGCACAGGGCTTAACCGGG







GCATCCTCCTGTAAACCCATCCTGGGGCTACACGACTGCAGCCCA







GGAACATTTCTTTCTTTCTTTCTTTCTTTTTTTTTTTCTCAGCAA







ACTTTTAAGAACTGTGAGCAGAGGCCAGTAATTTTGGAGACAGGA







CCTCGGCCTGAAAGTGATAGATGTCTTTGTTGTTACTGAGTGTGG







GCCAGGTGTAAGGTTTGTAAACGGGGGATGAGGAACAGAGCGGGA







CCGGAGTGTCTCATTTTTCCTCTTCTCGTTTTCCTGGGGAGTTAT







AAGTTAGACATTTATGCTGCTTCCTTTGTCTCCACCTACACTGGT







TCAGCTCCTTTTGGCACAAAGATTCAACAAATTAAGGAGTAACTT







TTTTTTCATCCCAGCTCACTGCAAGCTCTGTCTGCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SPG21. In some embodiments, the sequences from gene SPG21 comprise or consist of:











(SEQ ID NO: 328)



TCCCCCCGCGCCCGTCGCCGTGGCGACCGCCGGGGCCACGCAGCA







CCGCCCCTTCCTCGCGACGTGGCCCCGCCCTCTGAGAACCCGGAA







GCGGGAGTCTCGCGCCAGCTGAGCGGCGGTCGGGCGGGGGGAGCG







CGGCGGCCCAAGCTCGCTCGGCCACGCGCCCCGCCCCCTCAGAGG







CCGGCCTCCCGCACGCACCGCGCAGCCTGCTGTGCCCGTGGGTCC







CGAGTGCTCCGCCGCCCGCCCCGACCCGGGCCCAGCCGCCTCCAC







GGCCCGCGCTCGTACTGGAGCGAAGAGCGGCCTCCTGAAGGAGGG







GAAGGGACGTGGGGGCGGCCACGGCAGGTGAGGGTGGGCGAAGCC







CTGGGAAGCGGCTGGGTGTGCGGTATGCGGGGTTGTAGGTGTGTG







TGTGTGGCGCGTGGCTGCCTTGGTTGGTGTGACTTTGTGAGTGTG







TTCTGAGTGGGCCGGTCCATGTGACCACGCACGTGATCACGTGCC







CTTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PKM. In some embodiments, the sequences from gene PKM comprise or consist of:











(SEQ ID NO: 329)



GTAAGGGCATCCCTTGGCTTCTTAATCTACGTACTCCAGACCCAGG







GCTTGGACCTTTGCTGGTACGGGTTAAAGTGAGGCCGACAGTGAA







GGTCCACGTGGAGAGAGACATTGGGAAGTTGTCAAAAAGGCGTTT







AGAATCAGGTTTGACTCATTAATTTCCTGAGACTCTGAAGGAGTT







CAGCCTCTACACCTCAGTTTCCCCTGGGAAAACTGGGAGTAACAT







TTTCCTCATGATGGTTGAAAGGATTCATGCTTACGTGGATGTGGC







ACACTCAGTAAGTGTCTAATCTCTGGCGAAATACCCAGAAGAAAA







GCCTGTGACTGAATATATTATTTTTGAGCACAATTACTTAGTCCA







CAATTGAGCATAAGGGCCTTGATCCATTGTCTTCCTACTGTCCCC







AAACGCCTACAGCTAATCGTGAGGAATCAGGCTCTCTGAATAACC







ACGTGTCTCCTGGAAACCAGTTCCCCAAGGGATCTGGTTTAATAT







TGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PKM. In some embodiments, the sequences from gene PKM comprise or consist of:











(SEQ ID NO: 330)



CGGTGCCGTCCCCTGTGGCCGGGATTAGATGGGCGGCCTGCGAGG







GCCTGGGAATGGCTCGGGGCCCGAGAGCTCGACCGGCCCTTGCCG







GGTGGCCGCCAGGGACCACGCTCCATCTCGCCGCGGCCGGGCTGC







ACGTAGCGGCCGCGCCCAGGGCCCACCCCGCTTCACCGGGCGATG







GCCTTGGGCCTCGTAACGGGCGGGATAAACCTCTGCAGGCTTTGC







TGGGGCCTCCTGGCCCTCGCCCGTTCCGCGCCTCTCCGGCACGTT







TCTTTCTTTTCCTTTCTTTTTTCTTTCTTTTTTTTTTTTTTAATT







ATCCGGCACATTTTTTAACAAATGCGTCCTGATTGTGGAACGCGG







AGGCCGCGGGGTGGGGTGGGGATCTGGTTACGGAGGGGGCAGGAA







ATCTGTCGCGTTCACTGAACGCAAACGGTGTGGGTCAAGGGCTGT







TTGGGGGTCAGAGTTAGAGACCAGGATGACTAGACGAGTCATAGC







CCAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NPTN. In some embodiments, the sequences from gene NPTN comprise or consist of:











(SEQ ID NO: 331)



GTGAGTCCAGCACCCTACAGTAGTAGTACTGTAGTCATTAGAGGT







GGCCCAATGACCCTTCCCTTCTGCATCCTTTCCCTCTTCCCCAGT







ATGATCACTCACCCCACCCCTTGTCTTCGTTTTTTTTTCAATCCC







ATGACCCTTCTGGTTGTAGTGTATGAAAACATCTGTGGCAACTTT







TTTTCTTCTTTTCTTTGCCGTTCTCTCTGCCTCCCTTATCCCCCA







ACCCTTCTCTGTGTCTTTCTTCTCAGGAACAAAGAACTTGAGGAA







ATCCGTGACTGTCCAAAATCATCTGTGTTGGGAAGAGTTGGGGAG







GGAGAACTGGGAAGCTTAAATTAAAACTCATAAAACCAGGCAGTC







CAAGGAGGGTAGTGTTGAAATGGTTTTCCCTCCCCTTTGATTTTG







TAGTAGGTTTCCTGCTATACTTTTGCTGATTTCTTCTCTGGTGCC







TTTCTATTTTTGTTTCTCTCCCCAGTCTAACTACTTTTTATTTCT







TCTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NPTN. In some embodiments, the sequences from gene NPTN comprise or consist of:











(SEQ ID NO: 332)



TTGTTGAGGCTTGCAAAAGACACTGCATTTCTAACAAAGCGTTTG







CCCATCCAAAAGAGAGATCCTGATTTGGCATATAAAACTACTGCC







TCTTCTGGTTATCTTTCATCACTTGCCTGAGTTTAAAAAGGTAAT







AAATGGTTTGTGTTCTAAGGAAAGAGGCTCTTCCCAAGACCTGAG







GCTTTCTCATTCCTACCAGCTGGGCCCATTGCTAAAATGCTTTCC







AGTGGGCTTGCTTCTGCCTGGCCAGCACCTGGGCTGCCCCAGCTT







GTAATTAGCCTGCCTTTGCTTCATAAGCTTCCCAGGGTGTGGTTC







TTCTTCTAAAAGCTTGTATGAATTGCTGTACTGTCTCTTTATAGT







TTAAAAATGAATCCTTTTTTGTTCTTTGCAACTCATTTGCTTTTT







TCTTTTCTTTTCTTTAAATTACTATTAACACCTGTTGATTCTTTT







TTTTTTTTCTGATTGGCCTGTCACTGCATTCCTGCTCCCCCTCCC







TCTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene COMMD4. In some embodiments, the sequences from gene COMMD4 comprise or consist of:











(SEQ ID NO: 333)



TACCTGGGTGCTGGTTATGTGAGGTGTACAGTTGATGAAAACTTA







TTAAGCTTTATAATAGGTACAATTTTCTGTATGTGTAGTAGACGT







CAATAGTTTTTAAAATGTCTTTTGCCACAGCCCGCAGCTGTGAAT







AATGCTATTCGCTTAACAGTGGAAAACGTGTCAATGACCTGAAAC







ACCCTGGCTCCCGGGATCCTACCCCCTTAAGTTAAACAAATGAAA







TAACTTCCACTGTCTTTCCTAATGTCTTAATTCGGTCCTCAGTTA







AACTGGCGGTTGGCGCCAGTTTTTTCCAGGGAAGGATTCTGGGAG







ACGGAGGAGGAGACGCCAGCATTTCGCCCCCTGCACCTCCAGCGG







GCTGCGGTCCGCTCCCGGCCTCACTCGAGCAGGCCCCGCCCTCCC







AGAGGCAGTTAGTCCAAGTCACGTGATCGTCGACTCAGCTGACCC







TGCGGGACCGGAAAAAGAAATTCCCGGGCCCTGGCTTCTTGGCGC







GATG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene COMMD4. In some embodiments, the sequences from gene COMMD4 comprise or consist of:











(SEQ ID NO: 334)



TAGGGAAAAGTCCACCACTCTCCCGCTCCCGAGACGGGGGCGGGG







GTACGGGGCGGGTAAGACAGAGCAGGCCGGCCGGCTTAGAGTCCC







GGTGCTTCCCTGGCGGAAGGAAGGGCCCCTGCCTCCCGGGGCAGG







AACTAGGGCTTGTCTGGAGCTGGGAGTCCTTTCAGGTCTTCCCCA







TCCCCAAGAGGACCTCCCAAGGATACCCCCTTCCCCAGCCCTGCC







GTGGGGCTTGTACAAGAAGGTGCTTAGAATCAGGCTCACTCTTCC







ACACTGTTAGGAAGCCCCTCCGCTCTTTCCAGAGCCAGAAAGTAG







TAGTTTTGGGGTTGAGACTTATCCATCCATCCATCCAATCCATCC







ATCCGTACGTTCTAAGCGCCTGGTCTATACCATGAAGTGTGCTAG







GCACTGGGAGGACTTGAGCTGCCAAGGGAAGGGGAAATCGGAGGC







TTGAATTGGAGTCATAGCTAAGGCTCCAGGGGCAGAGACCTAACT







GCGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MORF4L1. In some embodiments, the sequences from gene MORF4L1 comprise or consist of:











(SEQ ID NO: 335)



CTCCGCCTTAACCTCCCACGAAATCAAATCCCCGCTTTACCAACC







CGCAGCAGCCGGGGGCATGGAGAGAGAAGCGGGGGTGGGGTGGGG







TAGGGTAGCCGGCCCCTCCCCCACCCGGGGGGGGGGGGGGGGCGC







CTCCACCGACTCCGAACTACCAGTCCCGGCGTGCCCTGGGGCGGC







GCGGGCGCAGGGGCGCGTGCGCGGCGGGCTGTCGTTGGCTGGAGC







AGCGGCTGCGCGGGTCGCGGTGCTGTGAGGTCTGCGGGCGCTGGC







AAATCCGGCCCAGGATGTAGAGCTGGCAGTGCCTGACGGCGCGTC







TGACGCGGAGTTGGGTGGGGTAGAGAGTAGGGGGCGGTAGTCGGG







GGTGGTGGGAGAAGGAGGAGGCGGCGAATCACTTATAAATGGCGC







CGAAGCAGGACCCGAAGCCTAAATTCCAGGAGGGTGAGTGTGCGC







CTTTGGGAAAAAGGCACCTAACGGCGCAGGAGATAGAGGCGGGCT







CGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MORF4L1. In some embodiments, the sequences from gene MORF4L1 comprise or consist of:











(SEQ ID NO: 336)



TGCGTCATTGTGAGAAAGCGCTTTGTGAAGCGAGAGTGCTGCGCA







GGCGTTAGAGTGGGAGAGAGAGCGTTTGGCGCGTGGCACACCCTC







GTCAAGTGTTTGTTATTGTTCTGAGGAAGAGGGCGCGGAGAGAGC







AGCCTATTGTAGGGAGGCGACGGGAGCGCCATGGCGGCGGTTAGG







GGAAGTGATGTGGCGGCGGGGGGGGAGGGGCGGTGGCTGTTTCGG







GCGGTCCTGCGGCGCGCCCGGCCGCTTGGGCAGCTACGACGGGTT







TTTCGGTGCTTCCTGGAGCCCCGGGGTGGTTGCGTTCGGTGGCAA







CTCAGCTGCGGATGGGGGTGGGTTTGGCGCCACGGGGCGGGAGAA







GTGCGTTGTAAAATGGCCGTTGCAAGATGGCGGCGCCATCATGGC







CGCCGGCGTCTCGGTCCCGTATGGAGGCACAGCTTACGGGCAGAT







GCAGCGCCCCCTTCCACGACGACCAGAAGGTTGCCGGGGCCCTCC







GCAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene AP3B2. In some embodiments, the sequences from gene AP3B2 comprise or consist of:











(SEQ ID NO: 337)



GCTGCTGGCTCACCTTTCCTTCTAGGGGTTTGTCAGGAACAACTC







TTGTTGAATCCCCAAGGTAACCATCTCTGGGTCAGGATGCCTAGG







AGTCTGCAGGCATCTCTGTGGAAGAGACATCTGGGCTGGGGTGGC







AAGGCAGAGCTGGAAGAGCACCTTGGTCCTTTTTATAGCCTCTGC







AAAAACAAGTCCTCTTCAATGGCTGGCAGACCCCCTTAGTTCTTC







TTACGGTCCCTTGGGAAGGAAGGACTCATAACATGGGGACTTCCC







CAAACACAGGTGCTCAGTTCAACATATGTCAACTACTCCAGTTAG







GGGGCCTGGAGGCCCCACCTGGCTGGGGTACTGACCCACTGGTCA







TCTTAGTGGAAGAATAGAAGATGCATAACTGTACTTCTCAAACCA







CTTTGTGGCAAGTCACCTGGGGATCCCATCACATGCAGATTCTAT







TAGTAGGTCTAGGGTGGGGTCTGAAATTCTGCATTTTTTTTTTAA







GTGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene AP3B2. In some embodiments, the sequences from gene AP3B2 comprise or consist of:











(SEQ ID NO: 338)



GATAAAGGCCTGGGTGGGAGGGGTAGAGAGGCTGACAAAGGCTTC







CTGAGGGAGGGCCCAGTCTGAGAGGGAAGTACCTTCTCCCCAAGA







CTGATGATTTAAATTCTGACCTGCCCTTTGCATGATACCTTCCCA







GCCTGGGAGAACTTGTTGCTCAGTAAGCAGACCCAGGTGCTGGCC







TCTGTACCCACCATGGCCTCTGCCCTCATCACGACCTCTACCCCC







AGGAAAGCTGATGGGCATGAATGAGATCACAGAGAAACTCATGCT







GCCAGACACCTGTCGGAGTGACCACATTGTGGTGCAGAAAGTGAC







TGCCACTGCCAACCTGGGTCGTGTTCCTTGTGGGACATCTGATGA







GTACAGGTACTAGGCCACCAGTAGAGGGGAAATGATGAGCATGGG







GGCCCTGGTTTGAAGGAGGAGCCCCAGCTGCTGATCCCCTTCCAA







ACACAGTCACCCTTAGCAGCTCTTCCTCACCCCTTCTATTCCCAC







CTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SEC11A. In some embodiments, the sequences from gene SEC11A comprise or consist of:











(SEQ ID NO: 339)



GTGATTATTTAAACTCTCCAATCTCTTTGAGTCTTTGTTTTTCCT







CAAATTATAAATGTACAGATTCATGTAGAAACATAAGAAAATATC







AATAATCAAAAAGCAAAGTCACTTCTTGTCTCCTTTTTCTTGATC







TCCATAAATATTTACTATTAACTTTTTGGTTTATATTAGCCTTTG







TGTGTAGAGATAAAAATATTAATGAGTACTTTTTTTTGCCTTTTT







TTTTTTTGGAGACAGTCTTGCTCTGTCACCCAGGCTAGAGTGTGG







GTGGCATGATCTCAGCTCACTGCAACCTCTGCCTCCTGGGCTTAA







AAGATTCTTGTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGTG







TGTGTCACCACACCCAGCTAATTTTTTGTATTTTTAGTAGAGATG







GGGTCTCGATATGTTGCCCAGGCTGGTCTCAAACTCCCAGCTTCA







AATGATCCACCTGTCTTGGCCTCTCAAAGTGCTGAGATTACAGGC







ATGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SEC11A. In some embodiments, the sequences from gene SEC11A comprise or consist of:











(SEQ ID NO: 340)



CAGAACAACCCTAATATTCCCATTCTACAGATGAGGAAACATAAA







TGAGTTAGGTTATGTATCTAAGATTATACTGATGGTCGTAAGTGG







CTGGCTGCATAGTCCACCTTACCATCATACAAAAATTACTAGCGT







AACATTTCTGAAATGGATAGTTGCTAATTCTCACTAGTTAGATGC







CACATGGCACTTTTGCCACTGTCAGAACCTCTGCTCCTTTTGACC







TAGAACAGACTGTATGGATACAGAGTTATTACACCAGTAAGCAGG







TCTGAGATACGCAGTTCCCATAGAGAAGGAAGTATGAGTTGCTAA







GTGAAAGCTTCCTCAGTTACAGCACGAGAGATGCTCAGTTTGGGG







CCAAAGAGATCAGATCAGAACAGGTATCATTGTGCTTTGCCTGCT







GCAGAGCTGTGGTTGTTATTGTTGTTTTAATATGTCCCCGCTTTA







AGGAACTTTTCCTTTGATGGAGCTAAAAATACTTTTATAATCCAA







ATTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PRC1. In some embodiments, the sequences from gene PRC1 comprise or consist of:











(SEQ ID NO: 341)



GTTAGTACAAGAGTGAGGTCTTTGGTTTGTTTGTTTGGAGACAGG







TCTTACTCTGTTGCCCAGCCTAGTCTCAAGTTCCTAGGCTCAAAC







AATCCTCCCAACTTGGCCTCCCAAAGTGTTGGGCCACCACACCCA







GCCAAAAGTGGGGTCTTCATGTGGCCTGCTCACTAGTTTTTAAGA







CATCCAGACATTCAACAGGAAGTATATCCTTTTCTGAACAGATAA







AGCCACCTTTTCAAGACATTTAGCATAATTGCCATTCCTGTATGA







AAAAGCATTTGCAAATTTTATTGGGAAATATTGGTCTCTTGCTTT







CTGAAGCAATATGTCACTGTAGCCTCTAGCTGTGAAGCTTAAGTT







TTTATCAGGTGCTTACTTTTTGCCAGGTGCAGTGTCAAACCCTTA







ATATTATTTCACATCCTCATGTCAAGAGGTGAGGATACTAAAGCA







CATAAATGCTGAGTCATTTAACCCAGAGACATATAATGGTAAATG







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PRC1. In some embodiments, the sequences from gene PRC1 comprise or consist of:











(SEQ ID NO: 342)



AAAGCGGTAAGAAACCCAGTGGGTACTGGGTCAGCAGTCTTCTCT







TAGCACTTCTGTGTTAGTCATAAAGACTGTTGTTGATTTGGCCAG







GCTCAGTGGTTCATGCCTCTAATCCCAGCACTTTGGGAGGCCGAG







GCAGGTGAATCATTTGAGGTCAGGAGTTCGAGACCAGCCTGGACA







ACATGGCGAAACCCTGTATATACTAGCCAGGTGGTAGTGGCGTGC







GCCTGTAATCCCAGCTACTTGGGAGGCTGAGACAGGAGAATCGCT







TGAGCCTGGGAGGTGGAGGGTTGCAGTGAGCCAGAATCGCGCCAC







TGCACTCCTGTCTGGGCGAGAGAGTGAGACCCTGTCTCAAAAAAA







AAAAAAAAAAAAAAAAAGTGTTGATTTATAATACCTTTGTGGCTT







TGCATTATAATTTTACTTAAAAGGGGGGGATCTTTTGCAAACCTG







AGTGAATATAAAACTATGGCAGCCACCCTTAACAAGTTTCTTTCT







TTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MT3. In some embodiments, the sequences from gene MT3 comprise or consist of:











(SEQ ID NO: 343)



TAGGCCTGGCTCGGCCTCAGGCTTATTCTGGGACCCAGGCACATT







TCTGCTTCTCTGGCCTCACTTTTTCTTATCTGTATAATGGAGGGT







TAGGAGTCAAGGATCTGAAGATGCCAGGCAGGACAGCGGGGAAAT







GGAACACGCCCCTTGCCAGTGACACTCGGAGGGCGCAAGAGTCAG







TGTCGGGCTCATCGTGAGCAGGTTCTTTCTGCCCCCAGTTCAGCA







ACCCGTCTGAACAGATCTGGCGTCCTGGAGAATTCTAGAATGAAG







GGGAAGAGAGGCAGGGAAGAGCTGGGAAATACGCAAAGCGCCTTT







TTCTCCACTTTCGGAGATGGTACGTGCGCGCTTCCACGCAGTGGC







GGCTGCTGCGGCGAGCACGTCCCCTGCGGGACCCACGCGGGGAGT







GGGCTGGCAGTGCGCGCACAGCGGCGGCGAGTGGGTCGTGCACGC







GGATGCGGGGTGGGAGTGGGGGCGCACGCGCGGGCGTGGGCGAGC







GGGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MAPK8IP3. In some embodiments, the sequences from gene MAPK8IP3 comprise or consist of:











(SEQ ID NO: 344)



CTGTCAGGCACACCCCAGAAGTCAGGGGTCCTTGAAGCCCCTACG







AAGCCCAGCCCGGGGTCCCCTCCCCACGTGGGTGGACAGGCCGAG







TCCTGCTCTCTGGCTGCACAGCAGGAGGCTCCGAGGCCCCACCCA







GAGGCTGTCCAGATGCAGCCCCCCAGCCTCCAGGCACAGCCCATC







CCCACAGGGGTGCCTTCTGCCCTCAGCCCCACCCAGCCCAGAATT







TGGGGTGAGATGTTAGCTTCCTGCTCCACAGGTGACACTGGCCTG







GCAGTCTTCATGTAACCCCAAGGATAAACTTTCCAGAAGGAATAG







CTGGCTCCATCCCATCTCCTGCCCTCCTAACTCCCTCCTGCACCC







ACCCTAACCCAGCAGACCCAGCTCTGAATTCCTCCCAGCCTGCTG







TCCACACGGGCTTAACGCGCTTCTCTTCTCTCCCTTTCTCCCTCT







CCTCCCGCCCTGGTTGCCCATCTCCTGTGGGACGGGGACGGCTCC







CTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MAPK8IP3. In some embodiments, the sequences from gene MAPK8IP3 comprise or consist of:











(SEQ ID NO: 345)



TAAGGCTGAGGCCCCGTTCCAGCGTGCGTCGCTCCTCCACCCCGA







CATGGTCTCCTGCTTCATGAGCCGTTTGCTTTGTTCTGCATGATG







GGGACAGTGTTGGGGCCGCCGGGGTCAGGGGGGCAGCCCTGAGTG







AAGCTCGCTCTGTCCAGGCTGCCTCTGGGCTGTGGGAGGTGGCGA







CTTCCCCAAGGGACAGACTTGAAGGCCCGAGGAGGGCAGCCCCAG







CCTCTCTGAACCCCACAAGCGCCGGGCAGACCCTGCCTGTGGGGA







GGAAGGCCCATGTTTGAGGCAAGGGAGGTCATAATAGAGACAGCA







GGCAGGACCCCTGAGCCCAGGCACAGAGGCGTGTGATGCTGTTTC







ATGACTGCATACACAGTGGGGGAGTGTGTGGGAGGGCAGGACCTT







GGAGTTTCTTTCCATCCTTTTGGGGTTTCTGGGGCATGCTTGCAG







GGAGATCAGTCCAGGTCCACTTGGGGCCTCGTGGTAGAGGAGCCT







TCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene HMOX2. In some embodiments, the sequences from gene HMOX2 comprise or consist of:











(SEQ ID NO: 346)



CGGGGTTCCCGCCAGGAGCGACAAAGGACACCCCCATTGTCTATG







CATCGAGTATGTGTCGAAGGGGGCGGCTGGGCCCGGAGCGGCAAG







ACGCCTGTCCCGAGATATCGGGGTCCCGCGACCCACCTCGTGTCC







CTTGGGCCCGATCCCCTCCTCCTTCCCGCCTCCCCCGGTTCCAGT







CACCCGCCCCCCGGCCCGGGTCCCACCGGCTGGAGTGACCTCCTC







GCCGCGGCCTGGGCGGGACAAGTTCCAGGAGCCCGGGACCCCGAT







TCTGCGGCCGAGTCCACTGCAGAGGCGGGGATGGAGCTGCGCGCT







AGGCGCCAACTCCCTCCCCGCAGCTCCGGCCCCACCTGGCAGAGC







GCCGCTCACGGAAGCCCAGCGTCGGAGGACCCCTGGCTGCAGCCG







CCAATCCCGACGTGTCGGCCGAAACCCCTCCTCCGGCGCAGTTGG







CTCCTCCTGGATCCCGCGGGACTCGGCGTCGTTTCCGGGTTTGCC







CGCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene HMOX2. In some embodiments, the sequences from gene HMOX2 comprise or consist of:











(SEQ ID NO: 347)



TATTGGGCGTCAGGCAAAGATAATTAAAGCTATGTCCATGAGTCT







TATCCCTTGAGGCCTTGTCCGCTTGATATTTTCTTTGCCTAACTC







ACTTTTAAAATTTTATTATTTTTATTTTTTTGAGACAGTGTCTTG







GTCCGTCGCCCAGGCTGGTGTGCAGTGGCGCGATCTCGGCTTACT







GCAACCTCCGCCCCCCGGGTTCAAGAGATTCTCCTGCCTCAGCCT







CCCGAGTAGCTGGGATTACAAGCATGTGCCACCAAGCCCGGCTAA







TTTTTGTATTTTTTAGTAGAGACGGGGTTTCACCAGGTTGACCAG







GCAGGTCTCGAACTCCTGACTTAAAGTGATCCACCCACCTCAGCC







TCCCAAAGTGCTGGGAGTACAGGTGGGAGCCACCGTGCCCAGCCC







TAACTCACTTCTGAAGTTGCTGCTACTTGCACTGATTTCAGTTTT







TTTTTGTTTTTGTTTTTTGTTTTCAAGGTGGAGTCTTGCTCTGTG







GCCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CDIP1. In some embodiments, the sequences from gene CDIP1 comprise or consist of:











(SEQ ID NO: 348)



GTAAAGCGGGGCCGGCGGTTGGGGCGCGGCATGGCGGGGCCCGTC







CTCCCCTCAGGGCGCTGCCGAGACCTAGCGCGGCCCTGGGGATGT







CGAAGCCGCTGCGGTGGCGGGGCCCGGGCGGGCGCCCCGCTGTGT







GCCGCGGGGTTGTGCGCCCGGAATGGCAGCCTGCTCGCCCGCACG







TTTTTCCGTCAGGGAAGGTTGTGGAAATTGCCATCTCCGTCTTGC







AGGGCGAGACGGGGAGGGGAGGGGTGGGAGTTGTTTTCTCAAGGA







AAGGGGGTCGAGGAGGAGCCCCGTGGCCGAGCCAGCCCCTGCCGA







GCCCCGCGGGTGACCTTGGCCGTCCCGGAGCCCAGCAGTGTCCTG







CACCCGCGCCCGGCTCGCCGACGTGTGGAGTCGGGATGTGGCGCT







CGGCCGCGCATCCCCGGCGGAGATGGCGACGGCCTGGCCTAGCTT







GGGGCTAGGAGTCGCGCCTTCGAGGACCTTGGCTTAGCGAGTTCC







TTGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CDIP1. In some embodiments, the sequences from gene CDIP1 comprise or consist of:











(SEQ ID NO: 349)



TTCCCTATTCTCCAGCCTCGTGTACATTGGGCAAACAGGCCCCGA







CCATCCCACGTGCTCAGCCCCAGCTCTAGGCCAAATCGCCTCCGC







TCCCACTGACGTCATCACGGCGCATCACGTGCCTCCCGAGACCCC







ACCCCCCACCGTGACGTCCCGGGCCTCGGCCCTGGTCCAAAGTCT







ACCCGCCTCCTTGTGACAGAAGTGCGACTGCCAGCTGCCGAGGCG







TTCGGTCCTGCTGTTGCGGCCGCTGCCCCAGGGCTGCGGGGACGG







TGAGTCGACTGGACGCATTCGGAGACGGCTTCGCGCGGGAGCCCT







CCTCCCCGGGGGAAGGGTCTGGGCGCCAGCTCTCCTCAGGTCGGG







GCGTGACCTCGGACCGTGGCGCCCCCTCCCCGCCCCCGCCCGCGG







CGCCTGGGCGGTGCATGCCGGGCACCGTAGTCCCCAGCCTCTCAG







AGCGGGTTGTGGTGGGGGGGGTGACCGGGCGGACTACAAGTCCTA







GAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SEZ6L2. In some embodiments, the sequences from gene SEZ6L2 comprise or consist of:











(SEQ ID NO: 350)



GTAAGCACCTTGCCTGCCCCTGAAGAGCCACCACTTGGCTCTCCC







AGGACTCCATAACCTTCTCGCAAGGTCTCTGGAATCTCTGTCTTG







GGAATTTCAAACTCAGCTGCCCCCAGAGGCCATGTCAATGAGCAT







AGCAAGCCATGTGTATACAATAGGGAGTGGTGAGGACTGTGGCTA







AGTGGAGGACTTGTGCCCCATCTCAAGAATACGGCTCTACCCCAT







AGTCGAAACTGAGGCACAATTGGCAGATCTTCCAATTATTCAAGA







CAAGCCAGAAATTCAGATTTTTGTGTCATTTTACGACACCGTAGG







ATGAACCAAACACATTCACTGGCCCCCCAGCCCCTAGAGGTCAAT







GTTACTTTAAAACCCAGGATCGTTGCCCCTCCTCCCATTCGGCTG







CCTCTTCCACCCCTATCAGACTCCAACAACTGACCTGATCTCTCT







GTCTTCAGGATACGCGGGAGTATGAAGTTTCCATCTGAACCCCAA







GACT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SEZ6L2. In some embodiments, the sequences from gene SEZ6L2 comprise or consist of:











(SEQ ID NO: 351)



CTGGCTCAGACCCCATCCCCTCCCCCTCCTCTCCCTGCAGTTGCC







TATGAGGAGCTCCTGGACAACCGAAAACTGGAAGGTCAGTGAGAG







CACAGGTGGAGACCCAGGCTGGCCAGGTTGGGAGGGCAAGGCAGA







GGCTGGGGCAGGAGGCCAGGCCTTGGAGGGATGAAGCCCAGGGTT







TGAGGCCCAGGGCAGGAGGCTGGGAGAGCCCAGGACCGGGCCCAG







CCACAGCTCACCGGCCTCTCCTCCTCCTGTAGTGACCCAGACCAC







AGATCCATCACGGCAGCTGGAAGGGGGGAACCTGGCCCTGGCCAT







CCTGCTGCCTCTAGGCTTGGTCATTGTCCTCGGCAGTGGCGTTTA







CATCTACTACACCAAGTAAGTAGCCTGCTTGGGGACTGCCGGCCA







GGGCAGACGTCTTGCGAGCCTGGCACTGGAGCCTGGGTCAGGGGA







GCTCAGGGGCTTACCTGTTGATAAGCTCCTCTGTCCTCTCCCTTT







CCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ALDOA. In some embodiments, the sequences from gene ALDOA comprise or consist of:











(SEQ ID NO: 352)



GGAGGCTGAGGCAGGTGAATCACTTGAACACGGGAGGCGGAGGTT







GCAGTGAGCCGAGATCGCGCCACTGCACTGCAGCCTGGGCGACAG







AGCGAGACTCCGTCTCAAAAATAAATAAATAAATAAATAAATAAA







TAAATAAATAAATATAAATAAAATATAAAAAGTAGCATTGATGTT







TACAATATCCCTGAGCGGCAGGCGTTTCTCCCTTTTGCAGATGCT







CAGTGGCTTGGCCGGAGATACATCCAAGTGGTGGGTGTAGCCTGG







GGGTTCAATCCTCCTGTGGCGCCCCAGAACCCGGTGCCTCCTCCA







ACGTCCGGCATCTGATGAGGATCCGACCCAGGCGGGCGGCGGCGG







GATTCGCTCTTCCCCTTCGCTCCCCGCGAGAAAGCCCCGAGGGCC







GCGGCGGCGCAGAGCCGGTGACAGTTGAAGCTTAGGCGGGAAGAG







GGAGGCGCGAGGCGGGAAGAGGGAGTTTGGGCCTCGGCAGCCGCC







GTAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ALDOA. In some embodiments, the sequences from gene ALDOA comprise or consist of:











(SEQ ID NO: 353)



TGATGGGTGCTGACCGACTGGGGAAGCCCGGAGTGTGGGGACTGA







GGAGGGGAGTGGCCTGGGGCGCGCTGGAGCCTGCGAGGAAGGCGC







CGCCTCGGATCCCCCGCCCCCCATTCCCCTTCCCGAATTCCACCC







TCCGGCCCAGCCATGGCCTCAGTTTCCCCAAACAGGAAAGGGAAG







GAGGGTGGGCACCCCGGTCTAACGGTGCCTCTCAGCCTCTGAGAC







CCAGAACCTTCCTTCTGCAGCTCCCGGACTGACTGGCTCTGCCCT







TCCCCATGGACGTAAGTCCGGGCCCCTTCCCCTCTCTGGGCCCCC







TCAGCGCCCTCTCCTAACCCCGAATGGGCCTGTCTTCCAGCACGG







ACTGGGGAGGGGGCTTCTACCCAACAGAGACTACAACCTGCCAGC







TCAGGACGAGAGCTGTCAGGAAGAGTCCAGGAATGGACTTCCCAC







GGGAGGGCACATTTCTGGTATTCCTGGCAAGATAAGGAGTTGACT







AAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GPT2. In some embodiments, the sequences from gene GPT2 comprise or consist of:











(SEQ ID NO: 354)



CGCTGGGCCCCGGGGAGGCTGGGGCCGCTGAGCAAGGGAAAAACC







GCAGCAGCCCCAGCTGGGGTCCTTCCCACGACGTCCACCCCCATG







GCCAGCTCCTCAGTCAGCCAAGCCTGGCTAAAACGAGAGAATGCC







CCCTCCCGCCCGTTCACTTACTGGTGCCCAGCACCGGGCACTCAG







TGAGGCCTTGCAATACGGTTCACTTGTTGAATTGAATTCGTCAAT







TGTTGAGCGGGCCGTGCTGCCAGGCACCGCACGTTGTGTGTCTGC







CCTACTCTCGTGGAACCAAGGTCCAGTGGAGAGAGGAACATAAGC







GAATGAAGAAAGAAGCAATAGAGTCTCAGTGATAAGTTCTTGGAA







AAAAGTAAAACGGAAGAGGGTTGGGATGGGGGCGGTGGGAGGGAA







ACCATTTTAGGTAGGAGGGGAGACGGGGTGGGGGGGGCTCTGCGG







AAAGTTGGTTCTGTTCCCCAGTCTCTTTCTCCGTGTCTTTGGCCT







TTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GPT2. In some embodiments, the sequences from gene GPT2 comprise or consist of:











(SEQ ID NO: 355)



TGGGGAACCAGAGAGTTGGGCCTGTCGGGCTTTGGTAAGGGGGCC







CTGGGACTTTCTTCATCTTGGTGGTCGGCCGGCTGGCTAGGATCT







TGGCTTGAGTTTATAGCTTCATGTAAGGTGAGCCCACAGCCAGGA







AGGATTTCTGCCTCCCCACAACCAGGATGTCTGGGGTGGGTGATA







GAGGGGTCCCTAGGACCTAGGAGAGAGTTATAGCCTGGGTGGAAT







TCAGGAGGCAGAGCCAGGAGGGGTTATCTTAAAAAGTAAATACTC







CTAAGATTTGTAAGAAGCTAATAATCACCTAGCTGCTTGCTAGAG







AGAGTCTGACTGAGAATAGAAGTGGGAAGGAGGGTTCCTTGATCT







TGCCCTCACCCGGGTCACTAGTGTCCCATGAATGCATGGCAGTAA







GCTTCAGGGGGATCATGACACTTGGTCCCATTCCAGTGGGGACTT







GGGGGTTTTAATGAGCTCCTGGGCCTCCTGAGACCTTGTTGAGAC







ACTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GNAO1. In some embodiments, the sequences from gene GNAO1 comprise or consist of:











(SEQ ID NO: 356)



ACATAGCCCAGTTCTAGGGACTGCATTCCACCCTGTGTAAGAAGT







ATAGAAAATTACAGCTCAGCCAAGAACATTGATTTTTTTCTCCCC







CGAACAAAATAACCGAGTTTTCAGGATGAAAGCAGAGCTGCTGGT







TGAGTTGGAGGGTGGAGAGCAGAGCCAGGCCCACTCTGCCTTCCT







GCTGGTTGAGTTGGAGAGTGGAGAACAGAGCCGGGCCCACTCTCC







CTTCCTGCCCCAGAAAACTCTGGGGCCCCATGGGCTGCAGCTTGG







AGCTCCTGCTTTAGGACATCCATGTGGTAGAAGGCCCCACGGGCC







CCAGGAACCCCAACTGTGCATGCTCAGGTCGTGGCCCTCCCTGGA







GCAGGCTGTGGGCTGAGCTGCGGTCCTGCTGCACTGGCTGGGCTC







TCATCACAGTCCCCGCTAGGGGAGAGCCCTTGGCTGGCAGAGGTC







TTCTGTCCCCACCAAAGCGTCAAAGCCCACCATCCACCTCTCCTC







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GNAO1. In some embodiments, the sequences from gene GNAO1 comprise or consist of:











(SEQ ID NO: 357)



TGAGTGTCCCAGCGGGCGCATGGCCTGGAGCCGGGCAGTGATGCG







GGAGTGGAAGGGACTGGTGATGGGGATTGGCAGAGCAAGGAGGAT







TCTCGGCTGAGGTCACGCCTGCCGGGAGATGTTCTCCCATAGGTA







GAGGGTGTCAGGAAGGAATGAGAAGGGGCCTCTCTTCCTGCACCC







CAGGAGGAGAAAGAGGCCAGAGGAGGCAGAGGACGCAAGAGACAA







CCAAACAGTGGAAATTCCCAGAGGGCCGGGACCTTGTCACTGGCT







TGTCCTGGAGAGTGCCTGGCACTGCAAAAAGACAGCTTTCAAGTG







TAGCAGCTTTGTTTTTTTATAGGGAGCCCCTGACCGCATAAATAC







AAACCAAAACAACAGCCATATAGGCAGAAAAGAAATGGTGCCATT







TTGTTTTATTTTAGAAAAAATAATAAGCTTTCCATTCCCCATCTT







TAAAATTAAGAATAAGGAATATGTAAGTCTAATTAATATAATAAA







TGTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MT3. In some embodiments, the sequences from gene MT3 comprise or consist of:











(SEQ ID NO: 358)



TGAGCCCCCGCCCCCGCTCGCATCCTGCGCACTGCGCGCCCTTGT







ACCTGCAAAGAAACCCACGCCCTGCGCCTTCGCTCAAGGACACTT







GGGGGAAGGGCCCCTGATTCCCTATTCTTCACCTCGTGAAGGGCG







GGCATGCCTGTGTCGCGGAGAACAGGGAGACTTGGCACCCCATCT







CCTCGTGACAGGCGTGGGGACCCGAGTTCGTCCACATTAACCCTT







CCTGTGGCGTCGCCCTCTCTAGGTGGCTCCTGCACCTGCGCGGAC







TCCTGCAAGTGCGAGGGATGCAAATGCACCTCCTGCAAGAAGAGT







GAGTGCGGGGACCCTTCCCCTCTGCCGCCGCCCCCTCTGCTCTGC







GGAGTCGGTGTCTCACCACGCAGGATGTGGAGAGACAGCCAGGCC







CCGATCCCGTGGTTTCTGACTCTTGCTGGAATAGAACCACCCGGG







CAGACATTAAAATACAGATGCCCCTGCCCCACACCCAGGAATTGA







TGGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MT1G. In some embodiments, the sequences from gene MT1G comprise or consist of:











(SEQ ID NO: 359)



GTAAGGGACGCCCGGGTTCTGTGCCTTGGGATGCCAAATTCCCAG







ACACCATAGAGAGTGTCCCTGGGTTTGAGGAGGTCGTATTTTGCT







ATCAGAGGTAAAGGGGACTCCTTTATTGGTCCAGTGCTTTCCTGT







TGGCCAAGCTCCTGAGGGCATTTTCCTCCTCCCTGTTCCTCTATG







TCAGAGTTGAGGGTCCTGAGGCTCAAGGCTGTCCTGCTCCACGTC







ACCTGGTTGGTCACAGGGCTGCTGGCTGAGCCCCAATTCTCTAAC







CTGACTCTGAGCTACCGGATTGGATAGGAGACATTGGATAGGAGG







GGACATTGCCTCTTCCAGGTTCAGGACAGAAAGTCGAAGTCTTCC







TGGGCCATGTCTGCAGCCTGGGACTTTCCTTTGGAGTAGAAATAG







GAGGGTGCTTGGTTTTCCCAGCATGAATGGAGAGGACATGGGGCT







TCTCTTCCTCTGCTCTGAGTGGGAAAGGAGCTCTGAGGGCTGGCT







CTGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MT1G. In some embodiments, the sequences from gene MT1G comprise or consist of:











(SEQ ID NO: 360)



GGATACTGCCAGAGATGCGAAGTTCGCACCCTTGGCCTGCCCTTT







GCACACTACTCCCTTGCTATGCTCCCCGCTTTGGCAGATCCAGAA







AGTGGAGCACAAGACTGGGGTTGCCCTCGACTCCAGGAAAGGCTT







AGCTATTGACTAGAGACCTGGGCGTTGGTGAAGGTGAGGGGAAAG







CCAGAATACTGGGGTAGGCGGAACGCCAAGGGGCGGGTGTAGCAG







GCAACCTCAGGGAAGCTGGGAAGGCCTGGCAGGGTGAGAGAAGCC







GCACACGGGGGGCACAGCGCCTCCCTCGCGAGCCGGTGCGAAAGG







GGCCGTCCTGCGGTGTGCGCCCAGCTGCGCTCAAGGGACCTTGCA







CTTGGCCCATCTCCTGCGCACAGCCCAGGCCGGGACCGCGGGCGG







TGCGGACTCAGCGGGCTGGGTGCAAGGGCGGGGCGGGGCGTCTGC







GCCCGGCCCCGTCTCCTGACTATAAAAACAGCCGCTGGCTGTTGG







GCTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NUP93. In some embodiments, the sequences from gene NUP93 comprise or consist of:











(SEQ ID NO: 361)



TAATCTGAATAGTTATTAAACAGTATCAAAATCTTTTAACTACTA







AGAGGCCAAACATTTAAGATGGCGAATTTACTGTTCTGCTTGAAG







GACAGTACAATTGACAAGGGCACAATGATCCGAGATGCTTCTGTA







TGTTGTATCTACACATACTGTAGACTAAAATGGCAGAATCTGTCT







GCTGCTGTATAAAATCTTTTAACTACCATTTATTTTAGCATCATG







GCATGTGCAAATTCCTGCTGCTCAGTTAAGGGACCAGCTTCACAA







CTGGTGTTACAATCTGTGGCCACAGCACTGCATCACAATGCAGCA







AATGCATTTTCCACATTAACCAAACATGACAGCTGAAGCGATTAT







TAACCATGCAAGATGTCGGAAGTCATCAAAGATTCACATCAGAGT







AATTGCAGTGGTTTGGGTTGGGGAAAAAAAGTATCATAGCATTCC







TTGCCTACATGTTGTCTCTCCCCCTCTTAAATCTTAACTTGGTAC







AGTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NUP93. In some embodiments, the sequences from gene NUP93 comprise or consist of:











(SEQ ID NO: 362)



TAGGATTGGCCGTGTTTTGTAAAGAAATTAAGACAAATGCACCCT







GGTAAATAGAGTGGCAGCATGTTGTTGCAGTGCCTCTGAAAGACA







AGGTCTTGGTTTGTTTGAAAATTTGAGATAAATTTGGAAGCAGTT







AACTCATTTGATAGAAGAAGTACTTGATTCTTCTGAGAATTGTTC







AGATTTGTATCTTGCCATTCTAATATTTTTTCTTTTAAAAATGTA







TTGGGTGGGGCAGAATTTGCTTAAATATTTAAATAGTTGGTTATT







GGGATTTTTGTGAACTTCTTCTTTAAGTAGCAAGTAGTGTGGTCT







TTAGGATTTTTAAAGGAGATACATGTGAAGTGCAAAATTCTTGTA







TGAGGTGGAATATGAAAATAGAATTTATTTTATTTACTAAAACCA







GATCCTTCTTTGGGATTGCAGGTGAGAGTGAGTGAGTGCGTGTGT







GTGTTTGTTTGTGTCTGTGGTGTGTCTGTGTGTAGGATTCTTTAG







GAAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CNOT1. In some embodiments, the sequences from gene CNOT1 comprise or consist of:











(SEQ ID NO: 363)



GTTGGTTTTGAGCTCCTCACCTCAAGTAATCTGCCTGCTTCGGCC







TCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCACGCCCTGCCA







TAGTCTCCATATTTCACAGTTTTGTGTCTGTGCACTTGTGCAGTT







GTTCCTTGGTCCCTTGCTATTCTTGGAGGATTGGTTCTAGGACCC







ACTGGGACACCAAATTTTAAGTATGCTTAAGTCCTGTATGTAAAG







TGGCATATAACCTTTGCATATCCCCTTAAATGACATCTGTAGTGC







TTATGATACCTAATGTAGTGTAATGCTATTATGGAAATAACTATT







TTAAACTATATTAATAACTTTTTAAAATTTGTGTTTTTTATTGTT







GTATTATTATTTATTTGTATTTATTTATTTATTTTTGAGACAGAG







TCTCACTCTGTCACCCAGGCTGGAGTGCAGTGGCGCAATCTCAGC







TCACTGCAACTTCCGCCTCCTGGGGTCCAGTGATTCTCCTGCCTC







AGCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CNOT1. In some embodiments, the sequences from gene CNOT1 comprise or consist of:











(SEQ ID NO: 364)



AGTTCCTTTGTGATTACCATTATGGGTTCTGTGATGTGATCCCAC







CTAATTGTATCCAGTTAAGAAATTTGATCCTGAGTGCCTTTCCAA







GAAACATGAGGCTCCCCGACCCATTCACTCCTAATCTAAAGGTAA







GGTTCCAACTAGTTAAAGGAAAACTTATGTCTTAATATTTCAGTC







CTTTCTCTGATAAGTAGACTGTTCAGCACTTTCCTACCCAACATA







ACACGCACACTATTAGTTAAGGGTACTCTTTAAAGGAAATAATGT







TATTAAGCCTGATAGAAGGTATGATATTGTTGCTATTGCTGTTGC







TGGTGAATATTGGTTCACAGCATTTTTCTAGTTGCTAGTTTTCAT







ATTAATTTTGATATTAAATTTAGCTCTATTTACAGAACCACAGCT







TAGGTGTTGGGGCAGTCATTTGCTGGGTGTGCCCCACATGGCAAA







GAATTGCAGAACAAACAGACCTTTGTAATCTTTTTCTTTTTTTGA







GGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CHTF8. In some embodiments, the sequences from gene CHTF8 comprise or consist of:











(SEQ ID NO: 365)



GTACGTGCGGGAAGGGGCTCTTCCCGGGGTTGGGAGGGAGCCCAG







CCCGCTATGCTTGCGGGAGCGGAGTAGAACTGGAACCTAGGAAGT







AGCGGGAAGAGCAGAAAGGGGAGTTCGGGGTGGGGGTTAGGACCG







TGAGGTTTTGTTCCAGGTAACATTTGTAATCGTGGAGGGGGGAGG







CTTCTGGGAGAGGTAATTGGAAGGGTGGTTGGAGAGTCATTCCAG







GTTCTTTTGTGGGAGGGAAGCCGAGAGACGAGTGGGGTGGGGTCC







TGGAGGAGTGGAGTACTTGAGAACCGGGAACAATTTGGAGAACGA







GGGTGGTAGAGAAAGGACGTTTAAAGAGTAATCTTTGGGAAGAGG







GTGAACGGTTTGGAAGACCACTCCAGTGGGTGGTGTGGAGGAAGG







CGGTTTAAAGAATAGTTTCCCTGGGAGGCAGGAAAAGGGGTTAAA







ACAGCTTGTAATTTGGAGGGGGGTGGAGAAGAGGGGGGAAGCGGA







TTGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CHTF8. In some embodiments, the sequences from gene CHTF8 comprise or consist of:











(SEQ ID NO: 366)



CCCGGTGCTCGCCTCTCTCCGCCCCGGCCCCACGTGCGCGCGCGC







TCTCAGGCTCTGCCCCGGAAGTGCTCCTGGCGCATAGTCGGAAGT







GCGCAGGCAGTGCTCGCGGCGGCGGCGACGGCGGCGGGAGGTTCG







GTTGTCGCCCGTTGGCCGCGCGGCGCCGCGCTCGGCGGCCGCCAT







TGCAGGTCAGGCCGCGGGCGGGGGGCACGGGAGGCTGCGGGGAGC







GAGGGCCGAGCCGGGATTGGGGGCGAGGGCGCGAGGGAGCGGGAA







GCTAGGGAGCCGGGGCCGGGCGGGGCGCGCGGCTCGGAGAGAAGA







ATGGCGAGCCGAGGGGCGGGGAGCGCGGGCGGGGGAGGGGTGGCC







GGGAAGGGGGAGGGCGGCGGGAGGGCGGCGCGGAGGTGGGGGCAA







GCCCGGAGGGGCCTCCGGGGTGGGAGGGCCAGGAGTGTGGGGGAA







GGGAGCGGTGTGGAGACGGAAGGCGGAGGGAAGGAAGGGAGCGAG







CGGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CALB2. In some embodiments, the sequences from gene CALB2 comprise or consist of:











(SEQ ID NO: 367)



GACCACTGGATTTAGGGCCCACCCCAATCCAGTATGACTCATCTT







AACTAATGACATCTGCAATGACCCTGTTTCCAAATAAGGTCCCAT







TCTGGGGGAGCACTCCAACCCGCTACACCTTCTGAACTCCAGGGA







CCCCAGTTCTGCCTCTGACCAGGTCCCGCCACTTGCTGATGACCT







GTCACACCTCTGCCACCTCGTGCTTGCCCAGACATTAAGGACAGA







TTCGGAAGCTGCCAACTTCTGCTCTCCTCTGTCCTCTCGGACCCT







GTGCTAGTTCCTCCCAGTATTTTCTAGCTGCTGAATGTATGTTGT







TCATCTTGGATCTCTAGGTGCAAATGCCCTGAGGGCTGGACCCAC







ACCCCTAGCTCCACTGGCCTAGACACCTGCCTGTTGGCCAGCTGG







GAGAAGACATTCTCCACGAAGCCCCCGACATGCCCACCCCGTGCC







CCCCTTACTATTTAGTGCTGAGATTGATTTTTTCTCTCTCTTTTT







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CALB2. In some embodiments, the sequences from gene CALB2 comprise or consist of:











(SEQ ID NO: 368)



TAAGCCCAGCCCTGTCTCCGATTGTGTGGGATTTTCCTGACCTAT







GCCTTTGAGCATGCTGTGTCCCAGTTATGTATGCCATAAGCAGGC







CTCATATCGCTGGTCTTGACACAGCATTTTATTTGTAAGGATAAA







AAATAGACTGTCCCCTAAGAGTACCCTATCTCAATATCCTTGGGT







GTCTGGGATTGAGGGGTATTCATGAGTGGACACCAGGGGTTGATG







AATCCTCCTGCAAAGCTGCAAAGCTTTGTCTGCGTATGCGTATAT







TAATATATATGTAGTATTTTTTTGTCCCTAGGGACAGAGTCCATA







GCTTCCCTCAGGTGCTTAGGGGTCATGATTCCAGATTGGATGCCC







AGGAGGTTTCAAGTGCCCTGATTCTAGACCAGAGTTTCTCAGCCT







CAGCGCTGTTGGCATTTGAGGCTGAATAATTTGTTGCAGGGGCTG







CCCTGTGCGCTGTAGGAGACTAGCAGCATCCCGGGATCTACCCAT







GAGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene IST1. In some embodiments, the sequences from gene IST1 comprise or consist of:











(SEQ ID NO: 369)



GGTATGTGCCTACACGCCTGGCTAATTTTTTCTTTTTTTTGTATT







TTTTTTAGTAGAGACGAGATTTCACAATGTTGGTCAGGCTGGTCT







TGAACTCCTGACCTCAGGTGATCCACCTGCCTCAGTCTCCCCAAG







TGCTGGGATTACAGGCATGTGCCACCGCGCCTGGCATGGAACCTG







GTTTTATCTTGAAATGTTGCAAACGTTTATAAAGATACAGTATTT







CTAAATTCACATCAACATCTTATGATAAGGGCTAGCTCCTACTTG







TCATATGACTGCCCCGGTACAAGCATTAGATGCAGCCTCCACAGC







AGGGAGCAGATGACTTCCTTCTGGAGGCCCCTTTTTCTTTTTTCC







CTTTTTTTTTTTGAGACGTTTCGCTCTTGTTGCCCAGGCTGGAGG







GCAATCGCCGGATCTCGGCTCACTGTAACTCGAGAATCAAGCGAT







TCTCCTGCCTCAGCCTCCGGAGTAGCTGGGATTACAGGCTTGCGC







CACC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene IST1. In some embodiments, the sequences from gene IST1 comprise or consist of:











(SEQ ID NO: 370)



TGATACACCTGCCTCGGCCTCCCAAAATGTTGAGATTACAGGCGT







GAGCCACCGCACCCGGCCCTTTTTCTTTTTTTCTGTAGCCTAGGC







TGGAGTGCTGTGACGTGATCACGGTTTACTGCAGCAGCGACCTTC







TGGGCTCAAGCGATCTTCCTGCCTCAGCCTCCCGAGTAGCTGAGA







CCACAAGTGCTCACTGCTGCTCCCCTAATTTTTAACTTTTTTTTT







TTTTTTTTTGTAGCGATGCGGTTTCACTATGGTGTCCAGGCTGGT







CTGCAACTCCCGGGCTCAAGCGATCCTCCCGCCCCGCCTCTCAAA







GTGCTGGGAAGGTCCCTTCCAGGCTTAAGCAGCGATAATGGTTTT







CAAGTTAAAAACAAAGGTAACCAAGGAAAAAGTTTTCTCTGAATA







CCGAGATAAATTAGGGAAATCGCCAGAGACTGTGGTGCTGAGGAA







ACACTACTGAATTATACCACACAGACCGGAGCGATGCTGGTCCAA







AGGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GLG1. In some embodiments, the sequences from gene GLG1 comprise or consist of:









(SEQ ID NO: 371)


GTATGTATTAGATTATTTGCCCATCAGATATTACTGATTCAGAAAGAGA





AACTGCTCCTCCTCCACCCTGGCTCTTTCTAAAGCAGAAGTATTAATAT





ATTTAATTTTCTACAGGATATTCAAATAGAAGCCTTGCTGATGAGAGCC





TGTGAGCCCATAATTCAGAACTTCTGCCACGTAAGTGATGTCTGGAGGA





GCAAGGGTTAAAAACAGAAACAAATTGACAGGGACTCTCCCCTGAGTAT





CTCAGACTCACCTCTGTCTGCAGCATGTATTACTCTTGAGTGTTAGCCA





GCTTTGACAAGTTAAAATTAAAAATGTAAACAGGCCGGGTGTGGTGACC





AATACCTGTAATCCCAGCACTTTGAAGCCAAGGAGGAGCACTTGAGCCC





AGGATTTCAAGACCAGCCTGGGCAACATGGCAAAACTCTGTCTCTACAA





AAAATAGAAAAAATTAGCTGGGCGTGGTGGTACACACCTGTAGTTCCAG





TTACCTAGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GLG1. In some embodiments, the sequences from gene GLG1 comprise or consist of:









(SEQ ID NO: 372)


ATTTTGTGGGGCCAGAAATCAGAGAAGTGGGGAACTACCATTTTGTCTT





AAACCTTTTAGTACTCTATTTGAATTGTCATAATGTAAAATTACGTTTC





TCAACCAGTTCTTTTTTGCTTTCTAGTTTTCAGAAGTTCATTGCCTTCT





CTCATTTCGTCGTCATCTCTCACATTATCATTGTCATCATGGACTTATG





AGAGCAGTCTGGGTGTATAGCTCTTTAAAGGAAGGTGTTTTTTCAGGAG





CTAGTGTTGCACGGGTTTCTCTCTTTAAAGGCATTATTAGTATGTGTTC





CCCAGTAACAGTGGTTTGGCATCTGCTTTGAGGGCAGTGCTCTGGCTTA





TGAGCTTATAAACTGAAGATGGATATCAGTTACCCCTGTTTTCATTTTG





AACTACTTAGCATTTTGGGAAAATCTGCAAAGGCTTTGCATACTCTAAT





GTTATTGCTACATGTGTTTTTACCTCACTTTTCTTAATTCTGCCTTCTC





ATTTGTTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene BCAR1. In some embodiments, the sequences from gene BCAR1 comprise or consist of:









(SEQ ID NO: 373)


GTAAGAGCACCTGGGGAGGGGAGGGCTGGCCCTGGACCCACTTCCCCCA





CTAGGCCTCATCCTTTCAGGTCGCTGGGGATCTTACCTGCTGCAGGCAG





CTCCCCCGGGGCAGGCGCGGCAGGGAGGGGGCCTTGGTCCCAGCTGCTG





CGCCCCCCAGCACACACCCAACCTGCCCCTCTGGCCCTGTCCTCCAGAG





TCTCCCCATGCTGTGCTGTGGGGCAGGGGGTGCTAGGCTCTATCTGGTC





CTGGGCCGGATGCAGTCCCCTGCTGGGCCAACCCTTCTGAGGAGGCTAC





AGCTAGGTGGTGTGGGCAGCAGACTCCCAGGCCTCCGACGTGGCCTCTT





GTCCTGTGGGGCTAGGCGCCCCAGTAACGGGGGGTTCCTTGCCTGTGGG





TAGTGCCCTCCAGAGCCAGGGCCTCAGCCCCTCACCCCACCGGTGTCAT





CAGCTCATACCCATGCTCCTCTGTGGAGCCTTCTGGGTCAGCAGGCTGT





AGAGACGTT 






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene BCAR1. In some embodiments, the sequences from gene BCAR1 comprise or consist of:









(SEQ ID NO: 374)


CATTTTGCAGAAGGGGGAGACCAGCCACTGAGCTCCAGGGCGGGGGCCC





ACTTTGTCCCCTTCCTCCGACCTGACACCTGCCTCTGGGGCCCTTACCG





GGAGGCTGGGATGTATTCCTGAGTTCGCTTGTCCCCCCGCTTGATGCCT





GATAGTAGGGGAGGAGAGGGGCGGCACCCTGGCCCTGGGGCTGCCTGGG





TTCTGTGCGTGCACCTGGCAGTGGGCCTGTGCTGTGTTTTCTCTGCATT





GTATGAGGCATGTGTGTCAGAGACCGTGTGGGTCTTATGTCAGTGACTG





TGCCTCTGTTCTCTCCTCCTGGGCTCTGGCTGTGTTCCCCTAACCCCCG





GGGGACCTCCACGGCCTGCTAGTGGGGTCTCAGGTGTGCAGCAGGGTGG





CCTGTGATTAGCAGTGGGGCTGAGTCATTCCCCTAAAGAGCTTTTCCCC





CTGGTGGCTGCGTTGCTCTGTTTTTCTAACAGAAACATTTGGTCACTGT





GGCTGCCTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ABR. In some embodiments, the sequences from gene ABR comprise or consist of:









(SEQ ID NO: 375)


GTAGGTACACCGGCTTTTCTTTCTGGGTTTGGGGAGGGGTGACGGGAAG





GCCTGGGCATCGTGGGCCAGGCCCTGCTTGGGTTGGTCTCGAGAGATTG





GGACTGGAGACGTTTGGACCTGGCCGTTCTTGGTGCCTCGTCATCTCGT





CCTGGCCCTGGACCCCTCTCAGGCTCGGGCTTCCCTCCCCACCTGCTTT





GCCAACGCTGCCGCCCCTGTCTCTCCCCGTCTACTAGCCGTCTGCTCCC





CAGGCCTCCCAGACCCCTCCGTGCCTCTTTCAGCTTTCCCCTGTTAGCC





TGTCTCCTCCAGAGGACCCAGGAGAGGACCAGGCTCTCCTCCCCTCTGC





ACCCAGGCCAGGCAGTGGCTGCATGCGTGGCCCGTGGGCTCCAGGTGTT





CAGATGCTGCTCCCGCAAAGCCAGCCTGGGCCTGGTGCTCCCTGTGGGT





GCAATGAGCTGCCCGCTCCAGCCCTCCTCTGTTCACTCTGGAAGGGAAC





AAACTCTGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ABR. In some embodiments, the sequences from gene ABR comprise or consist of:









(SEQ ID NO: 376)


CTCCAGAACCTGCCCTGGACCCCTGGAGGGAGTGGGCTCAGTACAAATG





GTACCCAAGGAGGAGGGCCACAGGAAGTGACATCGACCGGCCCCCCCCA





CCTCCTTATTCCCTATAAAAAGCAAAATGGTGACTCAGAGAGGAGAGTT





GGAAGCGTCCTGCCTCTTGCTCCCACTGTTAATTTTTACACTTGGAATT





AGGCAGAAGGCAAAGAAAACAGCAGCTTGAAATACGCGGCTGACATGCT





CTTCATCGGGTAGCAACAGCAGAGCCGCTCCTCCCCGCCTCCCAGCAGC





CCCGGAGCGGGCAGGGCCCGGTGCCTGCTGGCTCTCCCCCGCCCCTCAC





CAGGCCCAGGCCCCCGCACAGCCCCCGCCCGGGCCAGGCGTGGATGGTG





CGGCCGGGGCCATGGCTGCCGGGGGCCGCCGCCGGCGCCCACTACGCTA





CCAGTCCCTGGCAGCTCTGGTGGAGGATTCTCAGTGGCCTTTCTTGTTC





CTTGTCTCA 






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene UBB. In some embodiments, the sequences from gene UBB comprise or consist of:









(SEQ ID NO: 377)


CATTGCACTCCAGCCTGGGAAACAAGAGCGAAACTCCGTCTCAAGAAAA





AAAAGGAAAGACCCCCCCTCCTTCTCCCGCCGGAAATACCCTCTTTCAG





GACGGCGCGCCTGTGCGGCGCACGCGCGCTCAGTTACTTAGCAACCTCG





GCGCTAAGCCACCCCAGGTGGAGCCCAGCAACAACAGAGCCACCGCGTC





CCCCACCAATCAGCGCCGACCTCGCCTTCGCAGGCCTAACCAATCAGTG





CCGGCGCTGCAAGGAAGTTTCCAGAGCTTTCGAGGAAGGTTTCTTCAAC





TCAAATTCATCCGCCTGATAATTTTCTTATATTTTCCTAAAGAAGGAAG





AGAAGCGCATAGAGGAGAAGGGAAATAATTTTTTAGGAGCCTTTCTTAC





GGCTATGAGGAATTTGGGGCTCAGTTGAAAAGCCTAAACTGCCTCTCGG





GAGGTTGGGCGCGGCGAACTACTTTCAGCGGCGCACGGAGACGGCGTCT





ACGTGAGGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene UBB. In some embodiments, the sequences from gene UBB comprise or consist of:









(SEQ ID NO: 378)


GGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACG





TGGTTGGTGATTGGCAGGATCCTGGTATCCGCTAACAGGTACTGGCCCG





CAGCCGTAACGACCTTGGGGGGGTGTGAGAGGGGGGAATGGGTGAGGTC





AAGGTGGAGGCTTCTTGGGGTTGGGTGGGCCGCTGAGGGGAGGGCGTGG





GGGAGGGGAGGGCGAGGTGACGCGGCGCTGGGCCTTTCCGGGACAGTGG





GCCTTGTTGACCTGAGGGGGGCGAGGGCGGTTGGCGCGCGCGCGCGTTG





ACGGAAACTAACGGACGCCTAACCGATCGGCGATTCTGTCGAGTTTACT





TCGCGGGGAAGGCGGAAAAGAGGTAGTTTGTGTGGTTTCTGGAAGCCTT





TACTTTGGAATCCCAGTGTGAGAAAGGTGCCCCTTCTTGTGTTTCAATG





GGATTTTTATTTCGCGAGTCTTGTGGGTTTGGTTTTGTTTTCAGTTTGC





CTAACACCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene LRRC75A-AS1. In some embodiments, the sequences from gene LRRC75A-AS1 comprise or consist of:









(SEQ ID NO: 379)


CTGGCGTAGCGATACAGAACGTCGTCTAGCGAGGTCGACTCCATGCCCA





GGTCCTGCAAGAGCAAAGGATGCCCAGAGGTCAGGGCTGGCTGCCCACC





CAGCTCGCCCACCATCCCCAAGAGAAGTAGGCACAGACCCCTAGAGGCG





ACTCTGCCTCCCAGAGCCCCGGTGGGGAGCATCTGCAGAACTTCCCTCA





GGGGCTGGGGCAGGCCTCTCCTGTTTGTCTTTGCCTCCAAGTTGAGGAA





GAGCAGGGACTGGCCTCTGCAGGCTCTGCAGTTTGTGCAGGTCCTGGCT





TGTGGCCCTCTCCTAGGACAGCCCCTGACCTTTGTCTCTCTGGTTTTCC





AGAGGAGGAAGCCAAGGGTGGCCTGCCTCGGTGCAAGCAGCTGCTTCTC





TATGGTGGCAAAATTTGGAATTAACTGCCTTGTGACAGGATGGTTTAAT





GATATTTATTTGTGTTTTCTTCCTGCTTCTGACGTTGTTTTTACTGCTC





CTTCTGTAG 






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene LRRC75A-AS1. In some embodiments, the sequences from gene LRRC75A-AS1 comprise or consist of:









(SEQ ID NO: 380)


TACTGAGTGGAATCTTCCCGATGGGAGGGGAGCAGCTTGGAGGCGAGTG





CTACTAAGTGTGGTGGGGCCGCGGCAGAAACCGCTGGAGCAGAGCTTCC





CTGGGTCTGAGGAAGGAATTGGGTTTGGGGGCTCAGGACACACCACCCC





AGAATATGGTACCCTGGCATCTGAGGAGGCAGCTGACACAGGAAGGTCT





CTCTGACTGCTGTTCTCCCCCAAAGCAGGCCCTGAAGAATCCTGTGACC





TTCATCTAAAGTAGGTCATGAGACCCCTGAGCTTAAAACGGAGTGATCG





GCTTTGGAGAAGTAAGGAGACATGACGTGCCTGCCCTGGGGTGGGGACT





AAACTTCGTGCCACTCCACGTGAAAGGGGGAGGTACGCCTGCTCCCTTT





CCTCAAGCCCCCCCCTCCCAATTTCTGCCATCCTTGGGTATGAGCTACA





CGACAGTCCCTGACCCTAGCTTGGTGCCCTGCACAGACCAGGCAATGCT





GACATTCCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ALDOC. In some embodiments, the sequences from gene ALDOC comprise or consist of:









(SEQ ID NO: 381)


GTAAGGCCAGGCAAGGCGGGAGCCACTCAACATCTCCGCTTCATTTCCT





TGGCTCTTCCCCTGCCATCCTCGTCCTGCTACCTGGGACTCCGGGATGT





GCCCTTTCGACCCTTTCCTAACATCTTTGCTCCTTTCCGCGTACTTGAA





ACCCCATGGCTCAACCTCTTCTGTTCTATCCCTCTTCAGCTCTCCCAGC





TGGACCTCAGCTTTTCCAATCCCAAATCCTCCTCTACTTGTTGGATTTT





TCCTTGGAGTCTGTCTCTCCTACCCAGAGCATTTCCTTCTCAGCCTGCT





CCCTCTCCTCCTAGGCTAGGTCCTCTCTCCAGTTCTCCCGCCTTCTCTG





CCCCCCGGTCTAGGTCTCTCCCGCTGACTGGTTAGCCTGCATCACCACT





AGCTCCCCTCAGTCTCATCTCTCTCTCAGGCTCTCTACTCCTTTCAGCC





TTGGTCCTCTGCCCTCCCGTCTGGGTGTCTGAGTTGTGGGGAATTGAAG





TGCTTCCTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ALDOC. In some embodiments, the sequences from gene ALDOC comprise or consist of:









(SEQ ID NO: 382)


CCTGCCCTTCCTCCCTTCCTCTGCTTTCACCAAAACTCTGGGCACTGGA





CTGCTTGTTCCCTGGCTCACCGCCTGGAGGAAACAGAAAGGCTTCATTC





TCTTACTTTCCCCAGCCCCCTCGGGGGTATACCCTGGCAAATATGGGGG





TGAGGACAGAGAAGCTCAGTTCATTACTTCCTGCCCTGAACTTGGGCAC





CTTGAAGATGCTTCTGCCCCACCTACTCAACCTGTTGTTACTGTTCTGG





TGCCTCCTCTTCACCAGACTAAGCTTAATCCCCAGCATCCAGCAAGTGC





CCGGCACGTGGTAAAGGGATTGTGGCAGAAATGAACGCAAGGTCATGGC





CAGGCTCCCCGAGGTGGGAGGGCAGTCCCTAACAGCCACGGATGCCTGG





CGGGGCGCTCACTCCACCTCGCCGCCCCGCCTCTGAGGGCGTGGTCTTG





CCCCGGACACCAGTCCTGGGGAGGGGGTGTGGTCAGGGCGGGGCATGCA





GGCCACGCC 






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GIT1. In some embodiments, the sequences from gene GIT1 comprise or consist of:









(SEQ ID NO: 383)


GTATATGGTGCCTGCCGGGGCAGCAGGCCTGAAGGTGGGGTAGGGCGGG





GAGGCATTTGGTGTGCAGCATCTGGGGTGCCATCTCCCCGGGATCCCTG





CATGTTGGGGAGGTTGCTGGCATGGGCCTGGCCACCTCTCAGGTCTCTC





CTCTCCTCCCAATTCCCATCTCCCTCTGCCCTCAGCCTTGACTTATCCG





AATTGGCCAAAGCTGCTAAGAAGAAGCTGCAGGCGGTAAGTCTGCTCCA





ACAGTGCCCTGACACTTGAAGCTGGCCCCTCTCTGATCCCCCACCCTCT





GCTCTTGCTTCCTCTGCCTCTGACTCGATGACCTTTTCCAAGTCTTTGA





CCTGTCCCCTCACTGGCCTCAGCAGTCCCCTCTCCCTGACCTCCCTACA





AGGGGATGGACTGGTCCCTTCTCTCCCACCTTGGAGTTTGGAACATGAT





CCCTTTCCACTTTCCCATTCTGAGACTGTAGGGGCTGGGCAGCACAAGG





AAGGCCACC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GIT1. In some embodiments, the sequences from gene GIT1 comprise or consist of:









(SEQ ID NO: 384)


CAGGTGTGAGCCACTGTGCCCGTCCCTAGGGTATTCTGATGAAGTATGT





TTGGAGTGTGCCATATGCACCTGTTATCACTGCAGTGCCTTAAGGCAAG





GGGTAGTGCAGCCAGGTGTATCCAAAAAATCACCCTGGCTGCTGGGTGG





AACAGGATTGTAGGGACAAAACTAGAAGGAGGGAGATTAAGAAACTTAC





AAAGCCCAGGCAACAGGTGATTGAGGAACCTGGGGAGCTTCACTAGGAC





TGGAGGGTGAGGGAGGCTTCAGGTGACTGCCAGGTGGCTGACACTGGTG





GTGGACAAACAGGTCACTGAGAGGAAGAGCAAGCCAGAGGAGGAGGGGT





GGGGGACGCGGTGAGATGAATTCAGCTTTGTCCCTTCTGGGCCGGGTGT





GCGTGGAGATGGGTCTGGGCCTGATGTGAAAAGGCGTGTGCTGCCCTGC





TCCTGGGTAACTGCCGCCTCTGTCCTGTTCTTCCCTCTGCTGCCTTCCT





CTGGACTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ATP6V0A1. In some embodiments, the sequences from gene ATP6V0A1 comprise or consist of:









(SEQ ID NO: 385)


TCTTGCTGCAGATATTTTGTTTGAGCTTTTTTGGTGTCATTTGCCTACT





TTTTACAGGGAGAGAGAGAATTAGGCCAAACTAGACTTTTCCTTAAGAT





ACCACCTCCAAGCCCACTCTCCTTGCCTGACCCCTTGTAGCACAAAGCC





TCTGAACTGAACTATTAAATCTCATCTACTCATTATGTTCTTTTTAAAG





CAAATAAAATAGGAGTTGAGGACTCAAGTTGGACTTTTCAGTCTGTCTG





ACCACTTTCACTATATATGATTTTTTAAAAAAGAAATCACAAGGGTTGT





GGATGCCTTTAAAAAATATGTATCTTAATGCTCTAAATTCCTGCTGATT





TCGCTTTGTGTATATGCTGAGGAGGCCTGCCTCCAACGTGCTAGCCCAT





TTAACATGAACAGTCCTCCCCAAGAAGCATTCAGTAGCCTTGTGCTCCT





AACTTGTGACCTTGTGTGTGGCGTGGCTCCCCACTAAGTGTAAATTTGT





GTTTTCAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ATP6V0A1. In some embodiments, the sequences from gene ATP6V0A1 comprise or consist of:









(SEQ ID NO: 386)


TAAGATGAAAGCTGGCGTTGCCTTCGTGTTTATCCGGGCTTCTCTCTTCC





CATCCTTGTGATCACTCTGCTGACCCTGCCACACCCTGGGGCCATACACA





TGACTCCTGTGCCTCTGCATCTCACTGGCCATTTCAAACCAGTTCGTGAG





CCTGTGTTCTAAAAAGCTCATCTCCATTAACTGCATCTCATGTCGGTCAC





CTCTGTCTTTGTTTCATTGACTTTGCTGATTTAACACCTCTGAGAATGGC





TTTGAAAGGAGCTACAAGTAATGTCTTCACTGGCATTCTTGAAGTGACTC





CTCTTGGGGGTTAAGTCCCATTGGCCTCTGCCTTCCTCTCCTGTGATGTT





GTGGTTGATCTACTCCCAGCCACCGGGAGTAGCCGTTTCCTCCCTGCTCC





TCCTAGTGCTTGTGAGCAAACCTTACCCTTCCTCCCTGTCTGAGCCAGCC





TCTTCCTGCTGCCCTCCTCAGCTTGATGATGCTTCAACTTAGAAGTGGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PSME3. In some embodiments, the sequences from gene PSME3 comprise or consist of:









(SEQ ID NO: 387)


CCTACCCGACACTTGACCCCGGCAGTGTGAACCCCGCTGGGAGCGTCCAA





AGCCATGCCTCATCGCGGCTGCGCAGCGCACGTGCACAGCCTCGCAGGAG





TCTCGGCGATTGGCTGTGACGCAGTTTCCGGCGTGAGCGGCGAAAGCCGG





GAGGGCGAGCGAGAGAGCAAGCAGGCAGCAGGCTGCCGGCGGGCGGGCGG





ACGGCACAGAGGGAGGGAGCGAGCGAGCAGTGAGTAAGCCAGCAAGGGCG





GTCGGGTCCCGAGGTCAGCCGAGATTTCTCAGGTCCCTCCGGCCCCCTCC





CTGGAGTCCACAGCGCCTCCGGTGTCCAGAGGATCGGACACGGCCCGGCC





CGGCCATGGCCTCGTTGCTGAAGGTGGATCAGGAAGTGAAGCTCAAGGTA





GCGGCACCGGTCCGGCCTTTTTGCCCCTCGCTTTGATCCCCCAGCAGTCT





GACCGGCTTCCTACTCCCCATGGCGTCTTTGTCTCCCTGGGGATACCAG 






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PSME3. In some embodiments, the sequences from gene PSME3 comprise or consist of:









(SEQ ID NO: 388)


TAATCCCCCAGCCCAACAACGTCCCTGGACACTGCCCCTGCCCTTGGCGG





CTGGCCTGCCTAGTATCATAGACTAGGTCTGGGTGGGGGTTGGCACGTTT





GTGAGCTCACATCTTCCCTAACTACCTGTCTTTTGTCTGCTTATCAAATT





CAGACAGCTTCAGACACAGGAGGAAGGGAGGGAAGGGGGAGGACAGAGGA





GACAGGCAGGTGCTGTCCTCAAAGCCCTGCGTTCTTCTGAGATGGAAAAA





TGGATCCTCAAAAAAATAAAGTATTTGCAGTCTGGGGGCCTCTCAGCTTC





TTATTACAGTTACAAGGTGAGGGAAAGACAGTTGGATTGGGATTCTCCAT





CCTGCTCTTACCTCTGTCCCCAGGTACTGAATTGCTCTCTTTGTTAATTT





TAGGTTGATTCTTTCAGGGAGCGGATCACAAGTGAGGTGAGTGAAATAAA





TAGAAAAATGGTTGTTGGTTGAGAATTTCCCAAAGAGGAGATACCTGGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GFAP. In some embodiments, the sequences from gene GFAP comprise or consist of:









(SEQ ID NO: 389)


GTAGCCCTGCCTGTGGGCCAGCCTGCCTCCTCCAGGCAGCCCTTCTTGCT





CTGTACAAGAGAATGGGGGAAGGAGGGTGGAGGAAGCTGGGAGAATATCC





TCTCCAGGTGACTGACTTTCACCAGAGAAGTGAGATGGCTTGCCCAGGGT





CACACAGGCACTATTGCTTTCAAAAGTACCCATTATTATTACTGCTACTA





TTGCTATTATTAGTAGTAGCTGATACTGTTCTGTTCTAAGCCCTGTGCTT





TTCATGGATTACCTTACTATGAAGTAGGTGCTGTTCTTTGACAGATGAGA





GAGCCGAAGTCCAGAGAGGTTAACTAATTTGCTCAAAGTCACACAGCTCC





TAAGTGGTAGAGTTGGATTTCCAACTCTAGCACATCTCACTTGGGAGCCT





GCATTCCATGCCTCTGCCAGTCTGTTTTGGGTTACTGAGCCTCTACCATA





TGCCAGGCACCCCCCAGGACACAGAGAGGTGTCTGACCTGGAAGGTGTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene GFAP. In some embodiments, the sequences from gene GFAP comprise or consist of:









(SEQ ID NO: 390)


CTGCTCCCCACTGCCCCAGAAGGAGGGGTAAGGCAAGGCTGAGGAATGGG





GAGAAGGAGCCTGGGGCCCCAGTGGCCCTCTACTCCTCACCCCTCCTGCC





ACTCCAGGAAGCAGATGAAGCCACCCTGGCCCGTCTGGATCTGGAGAGGA





AGATTGAGTCGCTGGAGGAGGAGATCCGGTTCTTGAGGAAGATCCACGAG





GAGGTGAGGCCAGGGCAGAGGGAGGAAAGCTCAACACAGAGATTGCAACT





GAGAGAGGGAGAGACAGAGACAGAGAAGCTGAGACTGAGAGAGACACTCA





GAGAGAGGAGAGAAACAGAGATTGGCAGGTAGAGACAGGAAATGAAAGAC





AAACAGAAAGACACTAAGGGAGACGGAAAGAGACAAGAGAGCATTCGAAC





TCCTGGCCGAGGGGCAGGCAGAAGAGGACACAATGGCGTGGGGAACCCCA





GCTGTGGCCTGTACCCTGAGCCCTGGTACCGCTTCTCTCACCCTGGTCA 






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CDK5RAP3. In some embodiments, the sequences from gene CDK5RAP3 comprise or consist of:









(SEQ ID NO: 391)


TCCTCATATCTTGTGTCCCAGCTCAGGCTATAATCCCTCGATAACAACCT





CCTTCCAGCCTCCTTGTCCCCAGTCTTATCTCCCATGCATCCATCATCCC





AGCAGCCTATCTGTACAGGTTCCTCCCTGCCTCAAACCCCTTCTGTGATT





CCTCTGTCTGAAGGGATGAAGTCCAAGTTCCTTAATAAGTCTCCCTTTGA





TATGGCCTTTCCACCCTTACAGCCTCTCCTCTAGCCACTCGCTGCCTCAA





ACCATCTACGGTATAGAGGTTGGCCTGCCCGCGGAGCCGACTTCATCTTG





AAATTCTTCATCATTTTGTAACAAGCAGCTGTGCATTTCCATTTTGCACT





GGACCTCACAAATTACGTTGCCCGTCCTGCCTGCCTGTTGTTGCTTGCAC





CCCTCTTGCAGTTTGTGGCCACCTAAGCTTTGTCTGTGTAGTCTCCTCCA





CCTAAAACTCCTCTTCCCACATCACCCAGCTATCTTCAGCTCTTCGTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CDK5RAP3. In some embodiments, the sequences from gene CDK5RAP3 comprise or consist of:









(SEQ ID NO: 392)


TATTTGCAACGGCCTCCCAGATCGGCGCTAGGACCCCGCCAGCAGACGTC





TCAATCTGCGTGGAGAAGACCCAGTGCCCGCCAGGGGAAGCGGCTGCCAG





GCTGGGCTCTCGAGCGCCGCCTGTCGCAATCCCACGGCCGCTGCAGCGCA





CCCCCTCCCGTCCCCTGCCCTGAGCCCGCCTCGCGTCTCCAATTGGCTGT





CTCCATTCTCCCGCCCCTCCCGAGCTCAGCCATTCGGAGCCTGGGGTGGG





CGGGGCTTGAGGCCTGAGTGGAGCGTAAACCCTGATTGGCTGTACGGAAG





GCGGCGACGTGGCCGAAGGATGCCCGTTTGTGTCTAAACGGAGGCTCGGC





CACAACGCCACTGGATTGGTGGTAGGGCGGGGCGGGCCACAGTCTCCAGC





CTGAAGCGGAAGTGGAGGAAAGATGGAGGTGTGGGGACAGGAGCTGGGTG





TGCTGGGGACTGGCCGCGGACCCCTAACCTGTGTCTCCGGTCTCCCTCC 






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CBX1. In some embodiments, the sequences from gene CBX1 comprise or consist of:









(SEQ ID NO: 393)


GTGTGCTGATTGGAGGAGGAGATGTGTGGAATAAATTGGGAAAGGGGACA





CGGAGGGGGAGGGGCAGGCGTTTGCTGGGCCTGACTCTGGAAGGGGAGGA





GCTGTTAGGATAAGTAGGGCGGAGATCCCGAGAATATTTCTATAAGGTAT





GTAAGAAAAAAGGGGAAAGCAGGCCGCCCTTGCATCCTGATGGGTCCTTA





CAGTGGGGAGGCTGTAAGAATAATGTGCGAAGAAAGGATGGAATACGCTT





TTTTCAGTTTTCCAGGTTTCCTACTACCTTCACTCCATGATTCCTTTGGG





GAGTTAAAAAAGTCCAAGTGCAGTTCTTGCTTTGATTCAAAATTAGTTCC





GTACGTTTTTGTGTGGGATCGTAACTGGTCTCACAAAGGTGATGAAATTG





TAAAAATATGTAGATGAGTTAATAGTTGAGGAACGGCGTGAGGTAGTGAG





AGATTGTGAGATCGTGTGAGGTGCGAGAAATTGGGAGACATAGGAAAAT 






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CBX1. In some embodiments, the sequences from gene CBX1 comprise or consist of:









(SEQ ID NO: 394)


GATGACAGGCGTGAGCGACTGCCCCTGGCCCATATGTTCTAATATTCTAG





GTTACAACGCTCTACGCTGCAATCTCTCCACCGTTACAGTTTAAAAACTA





TAAGTCCCTACCACCCTCGCCTACTGTGCGTGCGCGGCGCGCGGCGCTTC





GGGCCTTGTAGTCTTGGACAGACCCCCGAGGCCCATGGTGCCGTGCGGCG





GGTCGTTGCGCCTGCGCGGTGCGAGCGGCGGGCAGCGCAGACTGCGAGGC





TCTTTTGTTCGGCTGAGGGGAGGGCCGTTGGCCGGGGCCTGCGGTACGCC





GCTTCAGTGAGGGACGCCACTGCGGCCACCCGGCTTGCTGCCTTCCTGGG





CGCCACTCCCCCAGGCGACCCGACGCGACGCGCCAGCAGCGCAGCACCGA





TTCCTCTCGGGCTCTTGGGCGCTGCTCTGAGGTGAGGAGCCCGCTGGAGG





CGGGAGAGCTGGGGGAGGGGGCGCGGCGGCGGCGGCGGCGGGAGCCCTG 






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PHB. In some embodiments, the sequences from gene PHB comprise or consist of:









(SEQ ID NO: 395)


GTGAGTCCGACTTGTGAGGGAGGGGAGAAGGGGCTGAGCCCGGGACGAGC





CAGGGGTTGCTCAGAGTAAGGGAGGTGTCCATGGAGGCAGGGTGAGGAAT





AATCCAGAAGCTATTACAAATGTAAAGGGCCGGGTGTCCCAGCCTCAGAG





AAGGAAGATTTAAATGCACTGGACGAGATCAGGGTAGTCTCAGGAGTTGA





GGTCTGGGAAGTAGGGAGGGAGGATTTGAGACTGGAGCGGGCAACGACGG





TGGGGCGGAGCGTTAGAAAGTTACATGCTGGCGTGATTTCTAGTTAGGTC





AACTGTGCTTATGCCCACCCCGCCTCAGCCCCACCCTCCCAGTTATTCCA





GAGCTCACTGTCCCTGTGCAGCTAGTTAGAGCCTTTCTCCCAAATGGGTT





CTTCAGTTATCTTGGCCCCAGGATGTCATCCAGCTCCTGCTTCCATAAGA





AGCATGTCGTTCTTAATACACGATGTTGACAAGCAGTATGGTGAGGAGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PHB. In some embodiments, the sequences from gene PHB comprise or consist of:









(SEQ ID NO: 396)


AAGACGGCCCTTCGTTTCCCCCAAAGGGTCTCAGCCTGCCGGTGATTTCA





CCACGACTTCCTCCAGAGTTCCACCTGTCCTCTTCATCAGCCATGAACTT





GCCCCTACTAATTTTTGCCACCAGCTGGACGCCCACCTCCTCTTTCCCGG





AGCCCTAAGGCTCTCCTTGCGGCAGTCTTAGCCTCTGTACAGGATAGGCA





CGTGCATTTAGCCCCAGAAAACTACAGCCCCCAAAAGGCTTTGCGGCAAC





ATCCTCGCGTTGATCACCAAAGAGAAAACGCTTTTCTCCGCGGGCCCGCC





GCTGCATAGCCTTTTGGGAGTTGTAGTTCATCTGCTAAGAGCCGCAGAAC





CAGGGTGAGGTTCTAAGCCACCCTTCCCAGAACTCACAGCGCTCTTCCAC





ACCAATAGGAAGCGTGGATCTTGGAAAGCGGGCGGGGCAGAGGAGCTCAT





GCGCAGTATGTGTGGTTGGGGAATTCATGTGGAGGTCAGAGTGGAAGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CLTC. In some embodiments, the sequences from gene CLTC comprise or consist of:









(SEQ ID NO: 397)


ATTACCTCAAATCCCTTGAACCATGCATGCTACCTTGCAGATGTTGCTCT





TTCCTTTAACTTCATCTGTATTTGTTCTGCCTCTTATTTGAAACAGTTGA





ATGGCATGTTTGTTTGAAGAAGAGAAGGTCCAATACTTGGCTATTAATAG





TCTCTCTTTAATTAACATAATGTAGATGTTATTGTGACCTTGAAGCATTA





ATGTTAAGTGAGGATTTGTGTTGATGAGCATATATGAATGTCAAGTCTTT





GGAATCCACTTGGTAACATGGTTTCTTATTATTTTTTCTAGCTCCAGAAC





CTGGGTATCAACCCAGCAAACATTGGCTTCAGTACCCTGACTATGGAGTC





TGACAAATTCATCTGCATTAGAGAAAAAGTAGGAGAGCAGGCCCAGGTGG





TAATCATTGATATGAATGACCCAAGTAATCCAATTCGAAGACCAATTTCA





GCAGACAGCGCCATCATGAATCCAGCTAGCAAAGTAATTGCACTGAAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CLTC. In some embodiments, the sequences from gene CLTC comprise or consist of:









(SEQ ID NO: 398)


TGGGTTTTCCTTAAAACTCTTCCTATGTTTTTGTTTTTTTTTGTTTTTTT





TTTGTTTGTTTGTTTGTTTTTGAGACGGAGTTTTACTCTTGTCACCCAGG





CTGGAGTGCAATGGCACGATCTTGGCTCACTGCAATCTCCACCCCCCAGG





TTCAAGCGATTCTCTTGCCTCAGCCTCCCGAGTTGCTGGAATTACAGGTG





CCTGCCACCACAACCAGCTAGTTTTTTTGTATTTTTAGTAGAGATGGGGT





TTCACTATGTTGGCCAGGCTGGTCTCGAACTCCTAACCTCATGATCCGCC





TGCCTCGGCCTCCCAGAGTGCTGAGATTACAGGCGTGAGCCACCACGCCC





AGCCAGCTCTTCCTATGTATTTATGCAAGTACAATAACTAGGGTTTACCT





TTTTAAGTATATTGATTAAGTTTGGTTAAAGAAAATAGATCTGCTAATAC





CGAACTTGATTTAAACCACTTTAATTACTTTTACTTGGAATGTATCTGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene AMZ2. In some embodiments, the sequences from gene AMZ2 comprise or consist of:









(SEQ ID NO: 399)


CCGCGGCCCCCAACAAATAAAGAAGGGAAAGTGCTGAGGGTGACGGCCCC





GGGGAGCGCTGCGGCTCTACGTCAACCTGCGGCGGCCGCCGACTCATTTG





GGGCCACGCTGGTTGCATTCGTCACGCCGGCGATGCCTCTCAAACCCGCG





GCCTGCCGAGGACGTTCCCACACGGGAGACCCCAGCGACGCGGGCGCATC





TGTGGCTCTCGAGAACCGGGCCGCGGAGCCGCCGCGAGCGCAAGCGAGGA





ATCGGCGACTGCGGGGGTGGACAGCTGGGGCTTGTAGTCCCCTCGCTACC





CTCTATTCTGGAAGAGGCGGGTCGCGGCCGCTGAACTCCAGCTCTGCGCC





TGCCCAGGCGGCCGCACGCTCAGGGGCGTGGCATGGGTGGGTCGTGAGTT





GGGCGGGGCCCACAGGGCGTGCGCGACGCAGCGGCGCGGCGCGTGGCGTA





AGGGGCGTGGCGCCAGTGGGCGTGGCGTGGCGCAGTGCGAAGGGACGCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene AMZ2. In some embodiments, the sequences from gene AMZ2 comprise or consist of:









(SEQ ID NO: 400)


TGAGGGCTGCCGCGGGTGGGTGGTATCGAGGCCTGTCGGGTCAGGGCGGT





TCGCGGGTGCTGTCAGAGCTGGGCCGGGGCCCCTAGGCAGGGTAGCCGGG





TCGTAGAGGCGGGGGCCGGTCGCGGTCGGTGGAGCGGGATGAGGATGTAG





GAGGGGCGGACGTGGCGGAAGCCGCGGGGTCCGCGGGGTCGGTGCCTCTA





GGGAGCCAGGGAGGCCTTTCCCGAGGCTCCTGGGGAAGAAGAGGCGAAGC





GAGAGTCCCTGGGGAACCCCCACTCCACTCCCAGCTGGAGACTGGGTTGT





GTCTGCATGGACCAGAGCCCACAGTGCGAGTTGCTATAGGCAACCAGCCA





GGGTGGCCAGCTCCTTCCCGTTTGCCCGTGATGTTCTGGTTTTGGGACCA





AAGCATCCTAGGCCTCCAGCCCACTGCAGTGACCGAATTCTGCGCCCCCT





GCCCATCTTCTCCCGCAGCTTCCCTAGATTAGGCTTGGGAGGCAAGAGG 






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TTYH2. In some embodiments, the sequences from gene TTYH2 comprise or consist of:











(SEQ ID NO: 401)



GTGTGCGCGTGCGTGCATGTGGGGCTCTCGGGAAGCTCTGTATCT







GGAGGGGTGTGTTATTCCGCACCCTCGCTATACACACATTTTCTA







GACCGTAAGTGCTGTGATGCACTAACCTCTAGGTGCTAGAACCCT







GCACGGCACTGAGTAAGTACTCCATAAATGTCTATCAACTTAGCA







AGTTAGTGAGTGATGCTCTATTGTGGACCCCTGAGGCAGTGGCTC







CTGAAGGAGGACGGGGCCACCGGCAGAGCAGGGCACTCAGAGGGG







TCCCCTGCCCGGGGAGCATGTGCCAGAGGAGGGGGACATCCCTAA







GGAACAGGCCAGAGGACCTGAGCTCTCCCTGGCTGCTCTGGAAGG







CGGAGGCCCCCCAGTTTATCCAGTCCCTAGAGCACCCCAGGCCAG







ATCTGGGGTGCGCCTGGCTTGGAGGCCATGTCCTTGCTGCCGCCT







CTCCTGGGGCCTGCGTCTGCTCCCACCCTGCCGCATGGGCCTTAG







CAAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TTYH2. In some embodiments, the sequences from gene TTYH2 comprise or consist of:











(SEQ ID NO: 402)



CCACCAGTGACTTCTGTGTGGCTCCTGACACCTTCATCCTGAACG







TCACGGAGGGCCAGATCAGCACAGGTAACTACACACTCTCAGGCT







GCTGCTGTGGATGCATAGGTGGCCGGACTCTGTGCCCCTACTTAC







CTCTTTCAGCCCTGTAGCCATCCAACCCCTCCTCAACCCTGAACT







TCAAGTCCAGCTCATGCTCTAGGCCATTTCCTAGAAAGTGCCTCC







CTTGGGAGCTCAGGGACGGGGAGGGAGGTCTGGCTTGGCCACCAA







GGATGATAGAGATCTCATCTGGTCATTTGTGAGCTGCCTAACGTT







ATGCCGTTGCCCTGCAGAGGTGACTCGCTACTACCTGTATTGCAG







CCAGAGTGGAAGCAGCCCCTTCCAGCAGGTATGGCCCCCCGAGGC







CTGCCCTCACCCTGCCCACAGCCGCTCCCCCTTGACCCTAAGCCT







CACCACTGACCAAGATGGCGGAGGTGGCAGGGCCTTGCTTGCCTC







ACTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene HID1. In some embodiments, the sequences from gene HID1 comprise or consist of:











(SEQ ID NO: 403)



GTGCGCCACGGGGGCCCCGGCGTCCCCATCCACCTGCCGGGCTGG







GGGCTGGGCAGGGCCGGGGGTACGCGAACTTGGCTCGTCGGGAGA







TGGGGGGACGGGCGGGCCCCGAGCGTAACGCCAAGGCCAGCGCCG







GAACGGGACCCCGCGGCCTGTACCTGGAAGCTCCATCCCCTGGCC







CTAGTCCCCCACCCTCAGCCTCCTCTCCTGGGAACACCCCTCTCC







CCGAAGCCGCTGCTGCCACCTTAGGCTGACGTGTGCCTACTCCCC







GAGCCGGCGACACAGCTGTCCGCCCCCTGTCCCGCCCCGCGGCGT







CCCCAGCTTCGCATCTCCAGCCTCCCGCCGCCCCCACCCACCCAT







CCACCGTCCGCTCCCCTCCCTGCCCCTCATTGCCCGCGGGTCCCG







GACGCACAGTCCCTGTTGACTCAATGCAAACAAAGCCTGGCTGGC







GTCCGCCCGGGTCGGAGAAACAGCTGGTGTGCGCGGCAGCGGCCT







CGCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene HID1. In some embodiments, the sequences from gene HID1 comprise or consist of:











(SEQ ID NO: 404)



AGGGGTCGGGACCGCGAGGGACTCTCGCGCTCAGCAAGGCGAGGA







GCTGCTTTCCTGGAGTATGGAAACTGAGTCAGCGCCTCCCTCCCT







CATAACCAAGGGCCTGCGGGACTGTCTGAACACGTGGGTTTGACA







GGTCAGAAAAAGGGAGAACCAAGAGAGTGGATGCTCAGAAAACAT







GAATGGGAAAGACAGACTGAGAAGGAGGAAAGTGGGATGGAGAGG







GCCAGCTGAGACGTGGGCCAGGAGCCGGAGCGGAGATGAAGGGTG







AGGACCGGGGCGGGGGACGAGCTGCGAGGAGGGTTTTGGGCCCTG







GGGACTGCGTGGTAGCAGCGGAGGCGGGTACAATGCTCCGACGCA







GAAGGAAGAGCGTCGAGGCTCCGGGTGGGACCTGAGAAGGCACCC







GGCGTGCGGCGGCGCTGGGTTGGAACCGCCGACTCCGCGGGTCTT







CGGCGGCGGGCGCTGCAGGTGCAGGAGGCGGAGCCACGCGGGGGC







GGGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SRP68. In some embodiments, the sequences from gene SRP68 comprise or consist of:











(SEQ ID NO: 405)



GTAAGACGAAGTTCGCTCTTTTCTTGAACGCCGGTTGCTCCAACC







ACTTAACCCCACTTGTCTGAGAAGGGTGTTGGCATGTTTGTTGGA







TTGCATGGAATTAAAACGGGCGCCGTAGCTCTTCCTTTCTGCTGA







GAATGTGGCTGTGGTATATGTGTGTTTCTTTTTTTTTTTTTTTTT







TTTGAGACAGGGTCTCACCCTGTCACCCAGGCCAAAGTGCAGTGG







CACAATCTCAGCTCACTGCAACCTCCACCTCCTGGTCTCAGTTGG







TCCTCCCACCTTTGCCTCCTGGAATAGCTGGGACCACAGGCATGC







ACCACCACACCTGGCTAATTTTTTTTGCATTTTTTGTAGACATGG







GGTTTTGTCATGTTGCCCAGGCTGGTCTCAAACTCCTGGACTCAA







GTGATCTGCCCACATCTGCCTCCCAAAGTGCTGGGATTACAGGCG







TGAGCCACCGCGTCCGACGCCATGTGTGTTTCTTAAGTAGTGGTG







CCAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SRP68. In some embodiments, the sequences from gene SRP68 comprise or consist of:











(SEQ ID NO: 406)



AGACTGCAGAAAAGCCCATCCTGAAGGTTTCAAGTTTTGTAAAAG







CTGACCTAGGATTGATTGATTCATTTGGACTAATCAGTAACATAT







TACTTCTGGGCTTAAGATTTCTCACTGTCATAGGCACACCTGGGA







AAAGGTCTAGTCAGACCACCAATTTTTGTGCAGGGAGTTTTGCCA







CTTATGTTTAAGAGCACGTTCTGGTTGGTCCAGTGGCTGGCGGAG







AAGGTCGGTGTACAGGTGGCCAGGAATCCTGCCTCTGCGTGTTGA







GAGGAGTTTCTTATTCCACTGTCCTTTCAGGAATCATCTTAGCCT







TGAATATAGATTTATAGAATAAAAGTGTAAATAGCAAAATATATT







GAAGGCTTCCTGTTTGGAGACATTTTGCCCTGCCCAGGGAGAATC







ATGAGAGCTTCTCCTTCAGAGATTAGTGGTCTGAGTCATTGCAGT







GTGGGCGTCGTAAGAAAAACACAACTGACTTAAGTCCCGCCCTTC







AAAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene EXOC7. In some embodiments, the sequences from gene EXOC7 comprise or consist of:











(SEQ ID NO: 407)



GTAAGCAGTGCGGTTCTGCAGCCCCCTCCTGTCCCTCTGAAGCTT







GGTGGGTGAGGTTCCCTGGGGACTGACTCTCAGTACCTACTGGGG







AGCCTGAGTCAGAGGGGACATGAGTTTTCTCGGGGTGCTCTCAAC







TCTCTCTGCTCCCTTCAAGGCCACTCTGTCTCTGAGCTTATCCGC







AGCACCTGCCCAGGCTAGAGCCCTGGATACACCCCCTAGGCTCGC







CCGGCCTCCCTGCCTTGCTGTCCCCACTCGGCCTTCCCCACTGTG







CAGGCCGCAGCTTCGTCTGTTCAGTAGGGTCTGGTAGAGGGGATG







TTCGAATGTAGGGGAGAAGGTGGAGAGGCTGTGGGGAGCCCATTC







TAAGACTCTGCTGTTGACAAAAGGAAAAAGGGGGCCTTGTAGAAA







CCACCCAGGGTGGGTGGGGGCAGGAGGGGACAGGGCGCTGGCATC







TCACTCTTTTCCTGCCAGCTTTTCTGCTGACATGGGCCAGCTCCA







TCCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene EXOC7. In some embodiments, the sequences from gene EXOC7 comprise or consist of:











(SEQ ID NO: 408)



TGCTGGTTGGTTGGAAGGTGTCCTTTCTGCCCCTGCCCCCCTCCC







AAATCCTTCTCTCGTGTCTGCCTGCTCTCCTAATCAGGCTCGCTC







AGCTGGCCTTCAGAGGGTAGCAAGTGTCTCAGGGCCCGCTGGGCG







TCTTCCTGCTGGCCCTGCCCTGGGACTGGAGCTCAGGGGGTTGGC







CTGTAAGCAAGAGCACCTTTCTGGGTCATTCCAAGCACTGAGGTC







CCTGGGCCCTCTCCTCCCGTCCCATTTTGGGGGCAAGGAGCCTGT







GGGCAGCCTCCAGCCTGCAGTTCTCAGCTCTCTACTTTCCAGGGT







GTTCCCTGGTCCTCAGAGGGTCCCGCCTGCTGTGTCTTGCTATCT







GTGTTTTGGCTGTCGTGGGGAGAGGTCTGCTTAATTCGGGTTGAC







ATGCCGTTTCTAACCTGTTTTTTGCACTTTGCATCTTTCTCCCTC







CTTTGTCTCTCCCTCTGTCCCCTCTGTGTGCACTGCCTTTCACTC







CGTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SEPT9. In some embodiments, the sequences from gene SEPT9 comprise or consist of:











(SEQ ID NO: 409)



CAGTCTTCTCCCAGACACTCCCCCGGCTCTGGCCTGGCGCCCGGA







GACCAGTAGCAGCAGCTCCCCACGGCCGTTCACTGTGAGGGTGGG







GAGGGCCGTGGGACCAGGAGGCCTTAGGGGGAAAGTTGGCCATGA







GGAGTGGGAAGCGACGGGTCAGATGCCAGTGACTGCTGGCTGGGG







AGCCGCTGGACGTGGCTGGCCTGTTTGGCCAGTGTGGCGGGTGCC







CCGGGGCTTCAGTCACCCCCAGCCACGTGTGGGGGTGCGGGATGG







GAAAGGTAAGAGTGTGTGGAGCCCAGTCAGCACTCAACATGAGGT







CCCGTGGGGGCTGCCTGAGGGCGTAGCTTCCAGAATTCAGCCCCT







GGCCCTCAGAAGGTGTCACCAAAGGCTTCTTTCCATTTGAGTGAA







TCCCTCCCAGCCCCCAGCAAGCCCTCGGAACGAGCCCACTCCCAG







GCGCTCTCTCCTAGTGTGGGAGTGGCCACGCCCCTGCTGGGAGTG







ACTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SEPT9. In some embodiments, the sequences from gene SEPT9 comprise or consist of:











(SEQ ID NO: 410)



TGAGCTGCGCCCCCATCCCCTGCCCCTCCTCTCCTGCTCCTTCTC







CCTTCCATGGTCCCAGCCAGCAAGCACCTGGGGTAGAGGGGACAA







ACCCAGGTGGCTGTGTTCCAGCCCTGGCTGCAGGTCTGAATGGCT







TTCTGGGGTGGCTGGCCATGCTCCCTGAGAGCCCAGCTGTGGCGA







TGTCTGAGCAGGTAGGTGGGGGAGCACCTAGGAAGCAGGGGTGTC







AGGCAGAGCACAAGGAGAGAGGGTGTCCAGGTCAGTTTCAGGACC







TGGCTGAGAGGAGGGGGCTCCTCACGGGCACCGCCTCTGGCAAGC







ACAGGGACAAGGGCAAGGACGGCATGGCCAGAGGTCCCTGGGAGC







CTCTTCCCCTCTCTTCTTCCTAGCAGCTCCCCCTCACTCTTCCCA







GGGACCCTGTCACTTTCCTTTAGCGTGTGGCAGCTCCTTGGCGTC







CCTCCCGTGCCTTCAGGTTGCTTCTGCGCCGGGCCTGCCGCTGGG







CGCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ANAPC11. In some embodiments, the sequences from gene ANAPC11 comprise or consist of:











(SEQ ID NO: 411)



GGCCTGCGCCCCACCGCCGCGGCCGCCCTGGGAGCCGGCCCGGCC







GCGGCCCCGGCCCCCGCCCCGGCCGCCTCGCTGGCTCCGGTTCAG







TTTAATGATGTCGTCCAGAGACATGTCCATTTTGTCGGCCATGGC







GGGCGCGGAATCGGGCATCGGCTCGAGCCCGCGCGTCAGCACGCC







GGCACGTCACTTCCGGCGCCGCGTGAGGCCTTCCGGTGCCACGGC







CTCGGAGCGGCTGCTGATTGGCCGCTGGCGGACGGCTGCGCGCGC







GGGACGGGGTGAGGCGGGGAGGCGCGTGCGCACTGGCGTGCGAGA







CTCGGCGGGCGCTGTTGAGGGAGTCGGGCCGCGACTGTGGTCGTT







TTTATACCTTCCCGCGCGGACGCCGGCGCTGCCAACGGAAGGGCG







GGTAGGGCGGTGCGTGATTAGGTTGGCGAAGGTGCGAGGGCGCAG







GGGGTGGGGCGGCGGCCGTTGGAGGCGGAGCCTACGCGCCCCTCC







CTCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ANAPC11. In some embodiments, the sequences from gene ANAPC11 comprise or consist of:











(SEQ ID NO: 412)



TTGGTTTTTTGGTCTCCACCTGCAGCCCCCCCAAGTCTGCACTGT







CACATTTTGCCAGTCATATCCTTTACAAGTTTTCTTGCGGGCCGG







GCGCGGGCCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGG







CAAGAGGTTCGCTTCAGCCCAGGAGTTTGAGACCAGCCGGGGCAA







CATCGTGAGACCCCCATCTCTAAAAAAAAAAATGAGCTGGGCATG







GTGGTGCACTTCTGTACTACCAGCTACTTGGGAGGCCGAGGTGGG







AGGATTGCTTGAGCCCAGGAGGTCGAGGCTGCAGTGAGCCGTGGT







GGCACCACTGCACTCCAGCCTGGGCGACAGAGCCAGACCCTGTCT







CCAAAAAAAAATTATTTTTGCAACTTATTAATGAAAACCAGTGTG







CCATTTCATTGATTTTTTTTTTTTTTTTTTGAGGCAGTCTCATTC







TGTCGCCCAGGCTGGAGTGCAGTGGGGCGATCTCGGCTCACTGCA







ACCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NARF. In some embodiments, the sequences from gene NARF comprise or consist of:











(SEQ ID NO: 413)



TGTCATATAAATGTAGCTGGGTACAATGGCTCAGACCTCTAATTC







CAGCCACTCAGGAGGCTATGGCTGGAGGATCACTTGAGCCCAGGA







GTTTGAGACCTTGCTGGGCAACACAGTGAGACCTCGTCTCTTTTT







TTTTATTTTTTGAGACGGAGTCTCACTCTGTCACCCAGGCTCTGC







AGTGGCTCCATCTTGGCTCACTACAACCTCCGCGCCTCCTAGGAT







CAAGCGATTCTCCTCCCTCAGCCTCCCGAGTAGCTGGGATTACAG







GTGCGCCCCACCACACCAGGCTAATTTTTGTGTTTTTAGTAGAGA







GGGGGTTTCGCCATGTTGGCTAGACTGGCGACCTTGTTTCTTAAA







AAAAAAAGGGCTGGGTGCAGTGGCTGACACGTGTAATCCCAGCAC







TTCAGGAGGCTGAGGCAGGCTGGTCACCTGAGGTCAGGAGTTCGA







GACCAGTCTGGCCAACATGGTGAAACCCCCGTCTCTACTAAAAAT







ACAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NARF. In some embodiments, the sequences from gene NARF comprise or consist of:











(SEQ ID NO: 414)



TGAGACTCCATCTCAAAAAAAAAAAAATGCAACCAGTGTATATTT







CATCATCCTATCTCTGTGAGCTTGTTATACACAAAAGTTTTCTGT







TTGTTTCATGCAAAGTAGGCCAAAAAAGTTTACAGATTTTCCACA







TGGGAAAATCATGGAGAGATTGTTTACATAAATCCCACAGTTCAA







GGGCAGAAAGAGGAATGGAAAGAGGAGTTAGAATTGAATTCTGCT







TACGTTTTATTAGTGTTTCATTTTATTGGCCTCACTTTGCCCTTT







AGATGGACTCCTGCTGTGTGTGATGGAGATTTCATAAGAGGGAGG







TCACCCAGGGTCACTAATGTCAAATCTCCTGCAGGGGAAGAAAAG







TTCCTGTGGCCACCTGTGTCTTTTCAGTGTCTTACTTCGTTTGTC







TGCAGGTGAAATTGCTCAAATAATGGAGCAAGGTGACCTCTCAGT







GAGAGATGCTGCCGTCGACACTCTGTAAGTGGCTTCTCTGGGGAG







AGTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene EPB41L3. In some embodiments, the sequences from gene EPB41L3 comprise or consist of:











(SEQ ID NO: 415)



GTGGCCATTTGTTTTCGTCCTTGGTGTTTCTGAATGTGCCCATGT







GCCTTTCCTTACCCTTTTTTTGGGCGTGAGTGTGTGGTTGCATCC







AGCTGGCTTTGCCTTCAACATCTCAAAGGTGTCTTCACTTATTGT







CCTTTGTGTCTGCTTGGCATATCTGTCACATGCCTTTAGTTGCAT







CTCAAGAATGTACTTCACTATTCGAAAGGCGATCATATTGAGAAA







TTTTAATAGAATTAAATATCTAAAAGCATTATGTCTCACCAGTTG







AGTCTAAGAAAGCAGCCAAAACCAACGTGAATGCGCTGCAATGCT







TTGGTTTTTGTTTTGTTAATTGCACTTTATCCTGCCTTCCGTTTG







TTTTGCGGATGTCCTGAAGCAGCAGCGCAGGTTCAAGTGCATGCA







CCAGGGGCAGGGGCAGGCATCAGAGATTTCAGTGGGAGAAATGAA







AGCTAATTTATATTTTTCGTCAGTGTTTCTGGTTATAAGCACTTG







CTGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene EPB41L3. In some embodiments, the sequences from gene EPB41L3 comprise or consist of:











(SEQ ID NO: 416)



GTGTCACTCATTGGGGCCACAGTTTGCAATTCCAGCATGACAGTT







TTTGAGGCAGTCCCCTTCCACCCCAAACCTTTCCGTTTCTCTTGC







TGCTTGGCTTTATGTTGAGAGCAGCCAACCCAGCTTCTTAATTTT







TTTTCATGGTTAAATCAATACCGCAGTGCAAAGTCAGCATTAAAA







CACTTTCCATTTTATTTCCAGCTCCCTGTTCCCAAGATGTGATAG







TGCTGCTGTGGCTTGCAATGAAGGGGAAAAAATATATATATCTGC







TTAAATCGGCGCCACCCTGATCCTAATAACCGTCTCTGCTTTACC







AAACAAGAGCACCTGGTTTTCTATTTTATTTCCCATTCTTTTCGT







TTCCAAACTCTGAGATTCCTTCGTTCTTCCTCCTCCCTGTCTCCT







CTGCCTACGATTTTGTCTAACAAGCTGTGAATGTTTATGTGCCTG







AGTTTGTGTCTCTCTTTTGTGCTTGACTCTGCCTCTCTTTGTTCA







CTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PSMG2. In some embodiments, the sequences from gene PSMG2 comprise or consist of:











(SEQ ID NO: 417)



GAGATGGCCGCACGGTCTCTAGCCCGCAGGGCTCTCAGAGTCGCA







GGCGGTGACGCCCGGTCGGGGGGCCCGGTCGCAAGGGACACACAG







CTGGGGGCGCAATGGTGAGGGCGGCGACGCACGGTCCGGAGGGGA







TGCGCGGCCGGGTAGGGCGTGCAGGGTTGTGGGGCGTGGGGGACA







CGCGGGGCCGGGCGCGCGGTCGGGGGGCGCAGGGGCAAGAGGGTG







TCGCATTTATCAGGGAAGTCCCCGGCCGACTCTGGCGGGGAACTG







GGCGCGCCAACCCGGGTCACCGCCCTGCACAGCGGGAGAAGTCAC







CCGCATTGACGTTCCCTAGGCGCTGGATCGCCGAAGGTCTGGTTG







AAAACTTGAACCCGCCTGGAGCGGATGCCTAAGAAAATGCAGGCC







CGCTGAAGCCTCCCCGCCGGGCCCTGGGCCCTGAAAGCCTCCGGC







AGTTTCCCGTGACCCCGCGGCCGCTCTGCGCGTTTTCCGATTTCT







TGCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PSMG2. In some embodiments, the sequences from gene PSMG2 comprise or consist of:











(SEQ ID NO: 418)



TAAGCACCGTAAAGACGGGGATCTTTACGGGTTTTCCTCACTGAC







TTCTCCCAAAAGCTAAACCAGCGCCTGGAATATAAACATGTACAT







AATTTGCAGGTGTTAGTTTTAGCGTCCTTCCGGATTCATTTGTAA







CATTGGTCCCTTCCCTCACTAAGGTAATGTCTCAAGTACCACACT







CATCAGAGCATGTGGAATGAATATCACAGTGGTAAAATTGAACTG







AGGGCAATTAGCCGTAGAAAGAAAGAATTCCGCCCCCCCAACCTA







TTAAATATGCAATAAAACAGATAATTCTTAATCACCCATTGGTAG







ATTAATTTAATCGATAAAATTCCACTGAGGCTAGTCCTGTGTGAA







AGCCCTACAGAGTGTTGAAAGCATCAACCAAATAGGCAATGTATA







TTAAGTTCCAGTAAACCTTTTACGGCAGTTTTATGTAAAATGTTA







GAAATCACGCTCTCAACAATTGCATCTTGCGGTAGAAACTACAAC







AGTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DTNA. In some embodiments, the sequences from gene DTNA comprise or consist of:











(SEQ ID NO: 419)



TAATTCAAATTTCCTACCAAAGAAAGTTAACCGTCTCTTGAGGCA







TTTGAATTATTTAGAACTGTTTTCAGATAATTTGCACATCTTTTT







TAGGGCCCTATACACCTGCCTCAGGAAAGAAAAAAAAATCTGTTT







GTCAGATGTAGGCAACACCACTCGTTAAGTAAGCCATTAGTCACT







CTATTTTTTAAAAAATAACTTAATTATCTCATCCACACCAGTTTT







CCATTCTCTGCCCATCTTCCTTTCTTTTTTCTTCCTCTCATTCTC







CGTGTCTCTGATATTCGCTCTCTTCCCACTGGACAATCAGCATTC







ACCCCTTGTGCTTCTGAGTCTTCCAGTGAGCTGTGCTGGTCTCCT







GGAGACAGAGAGCAGTTTGGGGTAAAGGCAAGTCTGTTTGGCTAA







TTTAAATTCTTGTAATGTGAGAGATGGGAATAGAAAGAGCAAATG







GCATTACAGAGATTTTTGTTGTTAAAAGGACACCTCTTCTCCATC







CCAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DTNA. In some embodiments, the sequences from gene DTNA comprise or consist of:











(SEQ ID NO: 420)



CTTCCATCCGGTTGAGTGTTCCTACTGCCACAGTGAGAGTATGAT







GGGATTTCGCTACCGATGCCAACAGTGTCACAATTACCAGCTCTG







TCAGGACTGCTTCTGGAGGGGACATGCCGGTGGTTCTCATAGCAA







CCAGCACCAAATGAAAGAGTACACGTCATGGGTAAGGCAAGGTCC







AGGCAATACTTGGAGTTGGATGTGTGAGTGTTGCCCAGAGTTAAA







GGGAATATCAAGGATGGTGGCAGAATTAAAGGGCAAACTTACCTT







CCATCCCAGGTCTAGTATTCAGTCCCCCTTCCTCCCACTCTCCAC







CCTACTGCGTGCTCTTACTTATTCTGTTGGAACCCAGTGTAGCTT







CCTGAGATTTAAAATATAGGAAGACTCAAAACTATGTTACTTCTT







GGAATATAGATAATTCAGTACTGATTTAAATAACAACTGAATAGC







AAGGACCTTCTGGAACATAATCTTACATTCACAGTAAGTTAAAAT







CTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MAPRE2. In some embodiments, the sequences from gene MAPRE2 comprise or consist of:











(SEQ ID NO: 421)



AGCCTGCACTCCATTTTCCAAATAGAACCCAAATATCAACAGCAT







CAACAAGCAGATTTGAAACCCCCCAAAAACACGCTATTATTAAAT







TGTTAATTGTATTAGAATTGCCTAGTTTAATTAGTAATATTCATG







ACAAAACTAGTTTGCCCCTTATCATCAATCGTGCTCCAGGTAGGA







GTACAAACACTGGGTGTAAAAAGCAGATGCACCCCAGTTGGTGTG







CAAGCCCCCGTTTGCAGGTAATTAATTCACAGTGTGGCGGCGCCT







TTTCAAGCCCCCTCTGCAAGCCGCGCCGGCCTCCTCCCTCTCGAG







CGGGGTCAAACCACTTAGTTTATTTCACCCGCTGGCACTTTCTTC







CCTGTCACGAATTCAGCCTGGCTTCCTTTGGCCTGAGACAGCGTC







TTCAGGCGGGTCGGCTCGGACCTGCCCAGTGGGGCGCCCACCTGC







ACCCGCACCCGCACCCGCACCCGCGGGAAGGGGCGGCGCTGGCCG







GACT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene MAPRE2. In some embodiments, the sequences from gene MAPRE2 comprise or consist of:











(SEQ ID NO: 422)



TGAGTCAAGTGCATTTAAAGCGGTACCGCCCTGGCGCACCCCGAC







ACCCCCTGCACTGAAGCAGTCCCTCCCAGGCCAGGGGCTGGCTGC







GCCTCCTCCTGCCGCTGAGGTCTGCAGCCCGCGGGGCTCGGAGCA







ACCGGAACCGGGGGTCCAGAGTCTAGCAAGAAGTTGTGCAAGGGT







CGGCTAGGCGGTCTCCGGGTGTGCACCCCGCCGCTCTCCCGCGGG







CCACATCTCTGGTTCGGTGCCCCAGCTCTGTGCACCCCAGGAAAG







TGGAGGGAGGGAGTTGGGGCTGCCACGCTGGCAGAAGCGAAGTGG







ACTGGTTCTGAGGGGTGAGGCTGGGGCTGTCCAGGGTAGGAACAC







GGCGTTGGTGAAGGAGGGGGGCTCGCGACTGCGCGACCGCGGCGC







ACGACCCTCCAGGCTCTAGCTGGGGCGCCGGCACTGAGAGAGCTG







GGGGCTCTGGCAGGGGCTGCGCGCTTGGGGGCAGATCAGGAAGTG







CAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CNDP1. In some embodiments, the sequences from gene CNDP1 comprise or consist of:











(SEQ ID NO: 423)



CCACTCCTTGTGGAACCTCAAGAGGGTGTCTCAGCTTCCCAACCC







CATTAGGGATTGGTCCTTCCTTCTGAAGGCTCCCATGTCACTTAA







AACTAGGAGCAAATCAATCTGTTATGCCTCTTCTCCAATTCAACT







GCCCTTTGCAAGCTGATTTTGCAGCGAACCTTCAGAGAGCTGGGA







AAACTCTACCCTCGGCCCCTGCACAGCCCTAGGAAGCTAATCCTC







GGCCGTAACATTCCATCACAAGGGCCCACAGCCACAGTGTGTGGG







GGCCTGTCATGTGAGGGGGCTGTCACCTGAGGGGGCTGTGCCGCT







GGCTCAGCTCTGCCTGCTGGCCGGGGCCCTACTCCCTCTCAGAGA







TGACACACAAGTGCCCCCAAAGAAGAACCCGAGGAGTCGCAGGCT







GGGCCAGGCTGCTGGCTTCCAGTGACATCACATGACTTTGGGGCC







ACTGCCTCATCCCGTATCTGCGCGTATGAGATGCATTGTCTCTTC







CTCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CNDP1. In some embodiments, the sequences from gene CNDP1 comprise or consist of:











(SEQ ID NO: 424)



TGAGTAGGACCTCCTCCATCAGAGTCCGTGCATTGGGTGCCAGAA







TTCCATTTGTCCCGGGAGCATGTGCGTTAAGCCCAGTTGGGCAGG







GCAGGCACCCAGCGTGGCCCCAGATGCTGCTCCTCATGGTGTTCT







TAGGTGCTGTCTTGGCCGAGGAATGTGGTATTTGGAATTATATTT







TATCAGTAATGCTTTTGGGTAGTTGTGTGTTCTTAACAATAATTA







AATGGCATTATTGTCAAGTAGTTCATATTCAAATTGTTCATGAAC







TCAGTCTTCTTAAATCAATTTTTTTCTGTACTTTTTCTCTTTAAA







AAAAATGAGTTTGCTTCCTGGAAGAATTTGCCTAAATAATGGTAA







CAGACAGTGTGCTGTTTGTCCTGGTCCCAGGTTCCCGACTTAAGG







GCTAGTTTGCAAGAGCTAATCACCGGGCTAAATCACCCCAGGCCT







TTCTCGGCGAGGTTTCCATGGTTCAGGTGCAAACCTGAGTGTGGG







TGTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene C19orf25. In some embodiments, the sequences from gene C19orf25 comprise or consist of:











(SEQ ID NO: 425)



GTGGGAAGAGGCCGGCGACTGGGGTGACCGCGGGGAAACTGAGGC







AGAGGTTGAGCCAGACCCTGAAGCCTTGGCCACCGTGTTTTTGGG







CGGAGGGAGGGTCAGGGCCTGGGGCGAGCTCAGAGGGGCGGGACA







GGGCGGGCTTCCCCGGGAGCGGGACCGCGACGGGTGCGTACGAGT







TCGCTGGGCGGGAGGAGCAGCCCGGGCAAGGGCGGAGAGGTGGAC







GCCCCGGTCAGCGCCCCCTTCCCCGCCTTCAGAGATGGGCTCCAA







GGCAAAGAAGCGCGTGCTGCTGCCCACCCGCCCAGCGCCCCCCAC







GGTGGAGCAGATCCTGGAGGATGTGCGGGGTGCGCCGGCAGAGGA







TCCAGTGTTCACCATCCTGGCCCCGGAAGGTAGGGGGAGCCCGGT







CCCGGGGAAAAGCGGACCTGAGACCCCGGCAGCAAGGGGACGGGA







GAGAAGCACACCCCTAACTCCTGACCCCACGCCCTGGGAACCCCC







GCAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene C19orf25. In some embodiments, the sequences from gene C19orf25 comprise or consist of:











(SEQ ID NO: 426)



ATGGTATGTAAACTGCATCTCAATAAAGCCCCTGAGAGAGGAAAA







GGGCCCTCTGCTCTTTCTGCCTTCCCAGGCATCAGCGGAGAGGAA







AAGGTGACCTCCAGCACCGTCCCCGAGGTGGGAGGACCCGCCTTG







GCCGGAAGTGGAGGCGCCGCGACCGCCCCGCCCCCGCTGTGTTCC







GGCCGCCGAGTGCTCATGCACCACCAGGCTCCCTTAGGTGGACAG







CAATTTTGTGCTCCCGTCAGCCACATGGGTGGCGTGTCCCCTAGT







GCCGTGTTCCCCTTTTCAGAAAGACGAGCCTCGGATGCTTTTCTA







CCCAGCCAGTGGAGCCTGGACTCGAACTCGCTCTCATCGTAGCGC







GTCCAGGTTCGGGTTCGCCTGAGAGGCTGAGATCCGAGTCTCAAG







GGCACTATCCCTTTAAGACGGGGCGGAGCCCCGCGATGAGCGGCG







GGCCGGCGGGAGAGGAGGCCGGAAGTGTGTGTCACGTGTTCGGGG







ACGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SHD. In some embodiments, the sequences from gene SHD comprise or consist of:











(SEQ ID NO: 427)



TCCCTCCTGGAATCCTTCCTAGAGCTGTCTGGGATTTCCTGTTAG







AAACCACATCTTTCAAATAAGGAAAACCAAGACTCAGGGAGTGAC







AGACCCTGCCCTCAGCCCCGCCGGGCTGCCAGGAGGCCCCAGTCA







TAGCAGTGTTGGTTCTGACTTCTGTCTTGTCCTCCAACTGTGCTC







TCCAAGCTATGACCTGTGCCTGACCAGGGGGCTGGCCACAAGAGG







AGAGAATCTGGGGTCCCATCCTTCCTGACCACCCCTCTCTCACTC







CCCTCATTAGGAGGAACTGGGCAAGCCCCCTTATGGGGAAAGAAA







AAAAACGGTTCTGCACTGGAAAATTGGCCAGTGGCTCCCTTTTCC







TCTCCCACCCCCCAAACCTCTCAGTGCCCTCACCCTCAGACAACG







ACAGCTGACAACAGGCAGCCAGTTCAGGCCAGACCCCTCTCACTT







CTGCCCCTCTTCCCGCCACCGCTCCCCCCCACCCCGCCCCCAGAA







GCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SHD. In some embodiments, the sequences from gene SHD comprise or consist of:











(SEQ ID NO: 428)



TGCGTGGCTGGGTGGCCTGGGGAGACTGGGTGGAGGGGAGGCTCA







GGATGGGCTCTCTGGATCGTGGAGAGCTTATTTTATGTGTGATCA







CAGGAGGGGACTGGGGCACCAGACAGACCCTTCCCAGTCCTCCAG







ACCTCCACCTGCTTGGGGAAGTCTGTTCTTTTTATGTATTTATTT







ATTTTGAGACAGTCTCCCCCTGTCACCCAGGCTGGAGTGCAGTGG







TGGGATCTCGGCTCATTGCAACCTCCACCTCCTGGGGTTCAAGCG







ATTCTCCTGCCTCAGCCTCTCCAGTAGCTGAGATTACAGGTGTGT







GCCACCACACCCGGCTAATTTTTGTATTTTCAGTAGAGACTGGTT







TCACCATGTTAGCCAGGCTGGACTCAAACTCCTGACCTCAGGTGA







TCCTCCCGCCTCTGCCTCCCGAAGTGCTGGGATTACAGGTGTGAG







TCACTGCGCCCGGTGGATATCTGTCCCTTTTCAATGCATAATGCT







GGTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene UBXN6. In some embodiments, the sequences from gene UBXN6 comprise or consist of:











(SEQ ID NO: 429)



GTAAGTTCATGGGAGGGGCCTGAGAGGAGACGGGTGAGACAGACG







CGTCCGAGCAGGTTGGGAGAAAGGGCTTCTCTGCGAGGCTTCTCT







GCCAGGTCACTGTGCCACGTGGGTTACGGAACTCTCCTTGGTGTA







GGGCTCAACAGGGTGGAGCCACGAGTCCATGAGCTCTTCGTACTT







GCACAGCCCTAGGCCTGGCATGGAGTGGCCAGGGCGAGGCTGGTT







CTGCTGTCCGCTCTCCCCAGGGTCCCGCCACGAGGGTGGCGTGGC







ATGGGGCACATCGCCCGGACTTCCTGTCCCTCTTGGGTTTAGGAA







TGGGATGGTGAGGCCTCCCTGGCAGCTTGTTAGGAGGAGTAAACC







AAGCGAGCCGACCAGCTCCCAGCAGGGTCCTGGCACACAGTAGGT







GCTCAGCAAACGAAGCTGTGACCTGAGGGTGGAGAGTCTTATGGC







AGGGAGGGAGGGAGGATGTGACACCCCTTGAGTCTCCTCAATTCC







TGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene UBXN6. In some embodiments, the sequences from gene UBXN6 comprise or consist of:











(SEQ ID NO: 430)



TTCTCTCCCTGCGGCAGCTCTAGCCCTTTCCAGACCCCCATCCTC







CTCCCACTCCCCCGGAGAACATTCTGGGCAGAAGGTGCAGACAGT







GCAGGGGCCTGCAGTGGGGAGCAGCTGGGGTGTGGGAAGAACTGC







GGGGAGCCCAGGGGAGACAGGCCACGGGAGGGCAGTGGGCCAGGC







TGGGAAGGGCTCTCTGGCCAAGGGAAGCTTTAGGATTTTGCAGTG







GGAGGGCGTTGTGCAGTGGAGTGATGCTGACGCTCCCTGGCTGAG







GTTCCAGAAGGGTCCCTGTGGCTGCCCAGGGGGGCAGGGTCCATG







GCTGGTTGGGGTGAGGTGCCCTGGGAAATGGCATTTTGTTGGAAA







GACCCAGATATCGGATGAGGCCGGGGTGGGTCTTCATGAGTCATC







TCCTCTTCCACCCAAGATGCTCAGCTGTGGGAGGACTAGATAGGC







AGGGGTGGGACGAGGAGGGAGGGCTGCCCGGGTATGTAAGCAGAG







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TNPO2. In some embodiments, the sequences from gene TNPO2 comprise or consist of:











(SEQ ID NO: 431)



GTGTGTGTAGGGGCCTGTTTCGGTGGGGCTTGGTTGGGTCTGATT







GGGGGAGGCCGCACTGAAAAGTTGAGCGGGGTGGGGGGAGGGGAG







CTCGTTGGGTCCTGACAGGGAGGGGCGTCAGACTTCGGCCTAGAG







GGTTTTCTTGGATCTGACTTCGAGGCGTTGCTGGGATCCGTTGAG







GGGAATGCCCCGGGGGGTGTTTACCCAGGCCGAACTGGGGGAGGC







TGCAAGGATCGGACCAGGGGGTTACTGACACGAGGTGGTGGGGGG







CTCGGCCGAACTTGACTGGGAGAGGCAACAGAGATCCGTCAGCAG







GGGTTACTGCTGACAGGGTCGGAGGGAATTTCAGGGAGAGGCTTG







GCCAGACCTGATTGGGGAAGGCGACTGGGATCGGTCCAGGGGGCT







CCTATAGTGGGTTGGCCGGGCTTGGCTGGCTCTCATTCGAAGAGG







CAGCAGAGACCTACCTGGGGACTGCTTTGGGCCTGATTAAGGGGA







ATCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TNPO2. In some embodiments, the sequences from gene TNPO2 comprise or consist of:











(SEQ ID NO: 432)



GAGGACCGGCTGGCTAGAGAGGCCAAAGAAACGGCAACTCTTTGG







TCACGTGACCGGACCTTGTCTCAGCCCGGGTTTCCCAAGTGCCAG







GGCACGTGTGAAGGTTGAACCTTCCGATTGGCTCGGCGGGACCAG







TTTGAACGGAGTACGGGAGCCGCGGCGGCTCACCTACGGCCGCGT







TTTTCTTTTTTTTTTTTCTTTCTTTCTTTTTTTTTTTTTTTTTTT







TTTTTAATTCTCTCTCTTTGGCTCCCTCCTTCCGCGCGAGTCTCT







GGAGAAGCCGCAGCGCGAGTTGCCGCCGCTGCTGCCCGGGGCCGG







GTAAGTGGGCCTCACTCAGAGCCCGACCCTCTTGGCCCCGGCTTG







CGTCGACCCCCGCCGGGCACCGAGCCTGCGCCGCGCGCGGCCCGG







GCGTCGGGGCCGCGCCCGACCGGGAAAGGCCGGGAAGCCGGTTGG







GCCCGATCCTCCTGGCAGCTAGAACGGGCCGGGCGGGGGAGGGGG







GAAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NFIX. In some embodiments, the sequences from gene NFIX comprise or consist of:











(SEQ ID NO: 433)



TTATTATTTAAACTTCAAACAAGCAAACAACCAAAAAAAAAAAAA







AAAACCAAACAAAACCGAGAGAGCCCATCCTTCTGTCACCTGACT







GAGTGGGAAAAAGGGTGAAGGGGTTGTGGGAGGCTGGGGAAGGGG







TCGCAAGAAGACCCATGTAGCTTTTAACCCTAATGTGGCCGAGAC







AAGCACCTTATTTGTGCTAACAAGAAGTGTTTTGTTCATTATTAC







TGTGATTAACATTAGTATTGGTGTCGATAACAAAGCTGAAATCAC







ATATTTAGGATTTAGGTCTGATTAAAAAATGTTGGGGTGGATGTT







CCAACTGGATCAGGAGAAAAGAAAATGAAAACAGCCTGGGGAGAG







GGAAGCCTGATCTGTTTCCTCACTCGCTTGCTCGTGGATGTCATT







TTCTGTCTTCTTGGGGGCGGCCAAAAATCGACCGGTGTCGGGGAC







CAGAGGCGGCCCCGCACGCCCCCGCGTGTGCGTCCACGGGCGTCT







GTGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NFIX. In some embodiments, the sequences from gene NFIX comprise or consist of:











(SEQ ID NO: 434)



TGTGTGGACGGCAACGTTGTCTGTGCGCGTGTGTGTGAGTGAGTG







AGGGAGAGAGAGAGAGAATAGGTGTGTGTAGAGGCTCCCGGTGCC







TCTGTCTGGCTGCTGAGGCTGAGATGGGAGCAAGTGGCTGGCGAA







GCTGGTGGTGGCTTCAAACCACACTTTCGTAGAACAATCGCAAGA







GAAAATTGTTGGGGGGAGGGAGGAGGAGGAGAAGGCGGTTTTCCT







TGTGCCCCCCCTTCTAACGCTGCTTTTCTCCTTCTCTCTTTCCCC







CTCATCCCGTCTTCCCCTCCTCCCGTCCTCCCTCGCCCCGCATGC







TCCCGGCTTGCCGCCTGCAGGATGAGTTCCACCCGTTCATCGAGG







CACTGCTGCCTCACGTCCGCGCTTTCTCCTACACCTGGTTCAACC







TGCAGGCGCGGAAGCGCAAGTACTTCAAGAAGCATGAAAAGCGGA







TGTCGAAGGACGAGGAGCGGGCGGTGAAGGACGAGCTGCTGGGCG







AGAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RHPN2. In some embodiments, the sequences from gene RHPN2 comprise or consist of:











(SEQ ID NO: 435)



GTAAGTGGGAGCAGCACACCTTCCAAAAGCCTCTGAGCCAGAGAT







CCTTCGTACATCCAGGGTGCTGCACAAAGAGGTCAGATAGCGTTC







TAGACTGGGGTGTGGCAGCCCCCACTTTCGGAAGTGAGAGAACCA







TCAGGTTTGGGGTTGAGTGAGGTGCTAGACTGGAAGGGATGAGCC







CATTTGTTGGGAAATACCTGCAGTGTTGGACAAAAAGGCATTTGT







GAGGCCGGGCACGGTGGCTCACTCCCGTAATCCCAACACTTTGGG







AGGCTGAGGCGGGTGGATCACCTGAGGTCAGTCTGGCCAACATGG







TGAAACCCCGTCTCTGCTAAAAATAAAAAAAAAAATAGCCAGGCG







TGGTGGTGCACACTTGTAATCCTGGCTTCTCAGAGGCTGAGGCAG







AATTGCTTGAACCCGGGAGTTGGACGTTTCAGTGAGCCGAGATCA







CACTACTGTACTCCAGCCCGGCTGACAGAGCAAGACTCTGTCTCA







AAAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RHPN2. In some embodiments, the sequences from gene RHPN2 comprise or consist of:











(SEQ ID NO: 436)



ATGCACCACCACACCCAGCTAGTTTTTGTATTTTTTAGTAGAGAC







AGAGTTTCACTATATGTTGGCCAGGCTGGTCTCGAACTCCTGACC







TCAGCTGATCCACCTGCCTCGGCCTCCCAGAGTGCTGGGATTACA







GACGTAAGCCACCATGCCCGGCTGGAATCATTCATTTCTTTTCAA







GTGGGTATCTTATGGTATTTTAGGGCATGGCTGGGAGCAGTTTTG







TTTTCTCTTCTCAAGACTGAGTGTTTGCAGGATGTCATAGAGTTC







ATGTCTGCAGCTCACAGTGTCATTGCCTGTGTCCCCAGCTCCACG







TACTGGCAGGTGTGCTGCAAGCTGGGTAGGTGCCCTGTGTCCCTG







GGATACCTTAACCGACACTCCTGGCCCTCCTCTGCAAGCTGTGCC







CTGATCCTCCCTGCAGGGACTGGGGATTGGGTCTGCTCACCTAGA







AGCCAGGATACCTGGCTGAGGGCACTTCTCTCCCTCTTCTCTTTG







AACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SCN1B. In some embodiments, the sequences from gene SCN1B comprise or consist of:











(SEQ ID NO: 437)



AGATGGGGCTTGGGAGGATGACTGAGGAACGTGTGTGTGCTCGTG







TGCATCTTGGCTAGAGGCAAAAGCGGGGTCTGGTTGACTCCAGGC







GTGATCCTAGCCCCCCACCCCTGTGCCCTCCCTGCCTGAGGTCAA







GGTGTCTGAGCCCATCTGTGTGCCATCTGTGTTTGTGGGTGTCAG







GGCAGGGGACAGAATCAGGGTCAGGTAAGGGAAGAGAGGCCCAGG







CAGTGACACCTTCCCCTCCCTGGCTACCCCTAGATCCTGCGCTAT







GAGAATGAGGTGTTGCAGCTGGAGGAGGATGAGCGCTTCGAGGGC







CGCGTGGTGTGGAATGGCAGCCGGGGCACCAAAGACCTGCAGGAT







CTGTCTATCTTCATCACCAATGTCACCTACAACCACTCGGGCGAC







TACGAGTGCCACGTCTACCGCCTGCTCTTCTTCGAAAACTACGAG







CACAACACCAGCGTCGTCAAGAAGATCCACATTGAGGTAGTGGAC







AAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SCN1B. In some embodiments, the sequences from gene SCN1B comprise or consist of:











(SEQ ID NO: 438)



CCGCACACCCCTCTGCACTCCTGCCCCGGGGGAGCCGCGCTGGCC







TGCAGTCTCACTCAACACCTCTGGGGCGGGCCTAGGGGTCCTTTC







TAAAGCTCTCAGGTGAGGCAGTGAAGGGCCACAGGTGCGGGGCCC







ACCCTCTGAGCAGCAAGGGCCTACAGCGCTTTTGAACGTCTCATC







ATTTTGCCTCAGCCACCCTGGACCCCTTTTCTGAGGGAGGTCCGC







CCCTTCTCTGATCCATCTTCTGCTCAGCATCAATTGCAATTGTCC







AGCGCCCTGTGGCCACTGGCTTAGAGTTGGGGAAACAGCCCAGGC







CTTGAAGCAGCTACAAGCCGTGGCAGAAAGGTCTCTCAGGGCCAC







GGCACACACATCCTCATGGGCAACACGCACTGCCCATCTTTTCTG







TGAGATGGGTGCCGGCCTCAGTGCCTTACACGGAGTCCCTTAGTG







AATCCTCTCCCCACCCTCTGGGGTAGGGGCTCTTCCTATCCCCAT







TTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SAMD4B. In some embodiments, the sequences from gene SAMD4B comprise or consist of:











(SEQ ID NO: 439)



TGTATGGAGTATGCAAGTCTCAAATATCTCCAGCCTGCACTCATT







CATTAATGCATGGAGGCACTATTCTAGGTACTGGAGACAGAGCAG







TGAATAAAACATTCAAGGTCCTGAAATTCATGGAATTTATATTCT







GATGGGGGGAGGAAACAAAAAAATCAGAGTGTGATTCATGCTGTG







AAGGAAATAAAACAGTGTGAAAGAGAGGGTGGGAGTGGCCACTGT







AGTAGACAGGGGGATCAGAAGAGGCCTCTTTTGGGAGGTGACAGT







TGAGCCTGATAACCAGAAAGATGAGAAGGCACCAGTTGTGGGAAG







ATCTGGGAGTGGAACATTCTAATCTAAGCATTGTTACATGCAGAA







AAAGGACCTCTGCAAGGTGGGGAAATTTATCACAGATATTTATTG







AGGGCCTGCTATGTACCAGGTGCTATGCTAGGAATTGGAGATGTA







GCAATGAATAATATAGCTACAGTCTTCCCTTCTCTCATGGATCTT







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SAMD4B. In some embodiments, the sequences from gene SAMD4B comprise or consist of:











(SEQ ID NO: 440)



TGAGCCCAGATCTGATTGATGAGAAGGAACCAACCTTGTGACGGT







CCGGGGAAGAGCATTTCAGGCAGAGAGGTCAGCAGGTGCAAAAGC







CCTGCCATATGTTCAGGGAATCGCAAGGACCAGTGTGGCTAAAGC







CTAAGTGAGGGAGAGGGGTGAGGGTTGGAAAGGCTGACAGAAGCC







AGATTACTTGCAGCCTAAGGGCCACAGTAAGGAGTTTCTATTTTC







ATTTAAGGGGATTGGGAAACTATTAGAAGGGAGTTTTTAAAAGCA







AGACAGCGACATGATCTGATTAATTTTTTTAGAAAGCTCACTTTG







GTTTCTGGGAGGAAAACAGAGGGTCAGGACAGAATCAGGAAGGAA







AGTTAGGAGACAATCCATTTGTCCAGATGAGAAATGGTGACATGA







ATGTGGTGGTGGTGGTGGTAGTGAAGATGTAGAGAAGCAGATATA







TTTTGGGTGTACAGAAAACAGGACTTGATGATGGGATTAGAGAAA







GAGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PAF1. In some embodiments, the sequences from gene PAF1 comprise or consist of:











(SEQ ID NO: 441)



GTATGTAGTAGGCTATACTATGTACGTTTGTGTAAGAGTACTGTA







TGATGTTCACATGATGTATATCTCAGAATGTGTCCCTGTTAAATG







GCCCATGCCCGTATTTATGTAAATACACCGTATAACACACACACA







CAGTGTAGGGCAACCAAACTGGTGCCATGGGAGCCATCCTTCCTT







TCCCCTGCCCTCAATACCTTGAGCTTAGCTGCCCTTATTCAATCT







GTATTTATTCCCATTTACTCTAAGTATTGATGGGGAACCAAGTCA







GACCAGACCCCTCACTGGGGGGCGGGGAAGTGGGAGGTGTGTCTT







TAACTGCATCTTCCACTTCTCTCTCCCAAATTCACCCCCTGCAGG







GTCCGCCTTAGTAAGCGCCGGGCCAAGGCTGGGGTTCAGTCAGGC







ACCAACGCCCTGCTTGTGGTCAAACATCGGGACATGAATGAGAAG







GAACTGGAAGCTCAGGTAACAAGCACCACTGGCTCAGGGCACCCA







TGGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PAF1. In some embodiments, the sequences from gene PAF1 comprise or consist of:











(SEQ ID NO: 442)



ATGAATGTTTTAAATGAAATGCTTAAACCCATGTTCAAAAATGTG







ACTCCAAAACTCTTCCCTTATGGCTCAAAGTTTACTGCTATGCCA







GCACCTCTGGTGTAGAGTTGGGAAGTCTTGAGTTTCTGTTTAAAG







CAGAGATTACAAGAACTGTGACCCCATAGGCCAGGCAGGAAATGT







AAAAGAGCAGTGGCTGGTAGCATCTGAGTGACCTGGAGTTCTGGG







CAACCATGGAGGGCACTATTCACTTCCAGCCCACTGTTGCTGTGC







TGTGGGGCCAGAAATTCCTATCTTCCAAAAGCCCCAAATCCAAAT







GATTTGACATCTCCTGGTTTCTCAAAGTGTTGGTAACTAACTGAC







ACATACAGTAATGCACTGCATGTTTCAGTCAACAATGGACTGCTT







ATGGTGGTCCCTTAAGATAGAATACCGTATCTTTACTATACCTTT







TCTATGTTTAGATACACAAATACTTACTATTGTATTACAATTGCC







TGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CLPTM1. In some embodiments, the sequences from gene CLPTM1 comprise or consist of:











(SEQ ID NO: 443)



GGCTCAAACATAGATGTCACTTGTCACCAGCCTGGCCAACACGGT







GAAACTCCGTCTCTACTTACAAAAAAAAAAAAAAAATGGGAGGCT







GACGCAGGAGAATTGCTTGAATCCGGGAGGCAGAGGTTGCAGTGA







GCCGAGATCGCGCCACTGCACTCCAGCCTGGGTGACAGAGCGAGA







CTCCGTCTCAAAAAAAAAAAAAAAATTAGCCAGGAGTGGTGGCGT







GCTCCTGTAATCCCAGCTACTCGGGAGGCCGAGGATGCAGTGAGC







CGAGGTTGCAGTGAGCCGAGATCGCGCCATTGCACTCCAGCCTGG







GGGAGAGTGAGGCTCCGTCTAAAAAAGAAAAAAAAAGAGAGAGAG







AGACGTCACTTGTCCAGGATATCTTATCCAGTCAGTAGCACCACA







AGGGTTAGAACCCTGGCCAGACATCCACCCAATTTCACGGGTGAA







CAGTTAACAGCCTCTGAGAGTCTTGTCCTCTCTGGGCCTCCGTAA







CACC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CLPTM1. In some embodiments, the sequences from gene CLPTM1 comprise or consist of:











(SEQ ID NO: 444)



TTTGGACCACTGAGGGAACGCGCATGCGTGCTAGGCAGGGCAGTC







CGAAGGCTTGGGGGTGTATCATAGGGTCTCATCGGAGGTTTGTTG







GCCGTAAGACCCCTGGAATTGGAACTCTAAAAACACAGAAAGGAT







ATGAGCTCAGGAGACTTGAACGAAAGAGTTGTCTTAGGAAGAGGG







GTCAGAAGACAAGGGTGCTGGGCAAAAGGCTAGTTCTTCGAATCA







GAATACGGTGGCCAGAAAGGTTCCTCTGACGAAAGAGGGGCGTGG







TTCGAAGCCGTACAGTGGCCGGTAAAGCTCAAGAAACATGGAACG







AAAAGGACGCGAAGAATCGGCAGGGAGAAGCGGACAGGATGTCCC







GAAAGGCTCATATAACCGAAAAGGCGGGTTAATGTGAAGGGACGG







AAGGGACAGAACTTGCGGGAGGCACATGCTGGCCGAGAAAGTCCT







GGAGTTCGGAGCATACAGTGTTTTTATTAGGTGGAAACAAACGGG







CTGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SLC1A5. In some embodiments, the sequences from gene SLC1A5 comprise or consist of:











(SEQ ID NO: 445)



GTATCTAATAACCCTCTGTGTGTCTGTCTCTGCAGAAATATCTTC







CCTTCCAACCTGGTGTCAGCAGCCTTTCGCTCAGTGAGTCCTCAG







GGGTGTCCTGAGGGGATGGGGGTGGACAGGGAGGGATGCCAGCCA







GGGGGAGCCCTTGCTACCTGATTTTGGTTAGTTCCTGGAAAAATG







CATGTCAATGAGAATACGGGCTCAGAGCCTCCTGTGAGTTTTAAT







TCTAGCTGTGTGACTCTGGGTAAATGTGTTTACCTCTCTGAGCCT







CGGTTTTCCACCTCTGGAAAATGGGGGTATTGATCATCCTGCCCC







ATGGGGTTGCCAGCAGATGAAATGAGATCACATTGAAAGGATGTG







GAAGGGCAGGCTTTGAGCATTGTTAGCTTGGCATGGGGTTGGGAG







GCCCTGGTAACGATGGACACTGACTCTTCCCAACCCCACCTACAG







TACTCTACCACCTATGAAGAGAGGAATATCACCGGAACCAGGGTG







AAGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SLC1A5. In some embodiments, the sequences from gene SLC1A5 comprise or consist of:











(SEQ ID NO: 446)



GAGAGAGTCTCACTCGGTCCCAGGCTGGAATGCAGTGGCTTGATC







TTGGTTCACTGCAGCCTCAACCTCCTCGGGCTTGAGTGATCCTCC







CACCTCAGCCTCCAGAGTAGCTGGAACTACAGGCACATGCCACCA







TGCCTGGCTAATTTTTGTATTTTTTGTAGAGACACGGTTTCGTCA







TGTTGCCCAGGTTGGTCTTCAACTCCTGGCTCAAGCGATCTGTCT







GCCTTGGCCTCCCAAAGTGCTGGGATTCCAGGCAAGAGCCATGGC







GCCTTGGCCTTAGCTACTATTTTCATCTTAAGGCAGACACTGTCA







CACAAGAGTAAGGGTAGCCTTGTAAACACAGATGCATATTCATGA







GATATGAAGCCGTCCCTTTTCTCCCTCCCCGCCACCGCCTCCCAA







CACACCCAGATCCTCCCCCATACTCTCAGAGCCGCTGGCAGGCCC







TCCAGGAGGAAGTGGCTGATGCAAGCTGTCCAGGGTATTAAGTTT







GCTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RPL13A. In some embodiments, the sequences from gene RPL13A comprise or consist of:











(SEQ ID NO: 447)



GAGAGGCTGAGGCAGGAGGATCCCTTGAGCCCAGGAAGTGGAGGC







TGCGGTGAGCCATGATCGCCCCACAGCACTCTGGTGTGGACAACA







CAGCGAGACCCTGTCTCACAAAATAAAGTAAGCCCGGACTGAGTG







CGGAAAGGCGGGCCTGGCGGGTCTGGTCTCCCCATGCGGGCCACC







AGAGGCCCTGCAGCCTTCAGTCGCTTGAAGGGGTAATGGCGCTTC







CACTCACAAACATGGCGGACAGAGCGTGTGAACGAGATGAACAGC







CCCTCAAAAATATGGCCGCCGAGGCTGGACGGCCGTGCCCCAGCA







GCACCGCCTCCGCGCCCCACGTGATCTCTCGCCGGGCACAGCGCT







GACCGCGGAGGTCCAACCGGAAGAATGTCCGGATTGGACATTCGG







AAGAGGGCCCGCCTTCCCTGGGGAATCTCTGCGCACGCGCAGAAC







GCTTCGACCAATGAAAACACAGGAAGCCGTCCGCGCAACCGCGTT







GCGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RPL13A. In some embodiments, the sequences from gene RPL13A comprise or consist of:











(SEQ ID NO: 448)



TTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTATGGGCT







CCGCGCGGGCCGGGGCGGCAAGGGGCCGGGTGGGATCCAGGCCGG







AATGGGGTCGCACCCTCTCTGTCTTGCATGTTTTGCGGAGCTTAA







CATCCATAATGAAGCAAAATGGAAGTCTTTTGGGATAAGGGGAAT







CTGTAGGAAATGCCGCGGCTCTCTTTAGGCCTAGGGTTGGCTACA







ATGTGGCATTTCCTTCTCGAGGCGAGGGTGATAGAGCTTCCAGCA







CAGGACAGGTATTTTGCAACTAGATTTTGCTTACCTTGAACTCGG







GTAGTGGGTGGGGGCCCTGGGGTAGAGTCTTGGCCGAATTGGACC







CTTGTGTGTCCTCAGCGATGAGGAACACGCGGAAGTGTGGCCGGG







CGGTCTCGGGGCAATGAGAAACTGAGTTTTGGCCAAAATGGAAGG







TATTTTTATCCCGCTCAGTGCGCGGGCTACTTTGATTTCACGTGG







AGGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NOSIP. In some embodiments, the sequences from gene NOSIP comprise or consist of:











(SEQ ID NO: 449)



GTATGCACCACCACGCCCAGCTAATTTTGTATTTTTAGTAGAGAT







GGGGTTTCTCCATGTTGGTCAGGCTGGTCTCGAATTCCCAACCTC







AGGTGATCCGCCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGG







TGTGAGCCACCTCACCTGGCATTTTTTTTTTTTTCCCCAGAGACA







AGGTCTCACTCTGTCACTCAGTCTGGAGTGCAGTGGTGCAGTGGC







ACGATCATAACTCACTGCAGCCTCAACCTCCTGGGATCAAGTGAT







CCTCCTGCCTCAGCCTCCCAAGTAGCTGGGACCACAGGCGCATGC







CACTATGCCCGGCTAATTTTTTAAATTTTGTTTTGTGGAGATGGA







GTCTTGCTATGTTGCCCATAGCAAGTTCAAGTCTGGTCTCAAAAA







CCATGCTGCTGCTGCTTTTTTTTGGTAGAGACAGGGTTTTGGCAT







GTTGCTCAGGCTGGTCTTGAACTCGTGAGTTCAAGTGATCCTCCT







GCCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NOSIP. In some embodiments, the sequences from gene NOSIP comprise or consist of:











(SEQ ID NO: 450)



GCCATGTTGGCCAGGATGGTCTCGATTTCTTGACCTCATGATCCG







CCCGCTTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCAC







CGCGCCCCGCCTGCATGGATGTATGTTTTTATTTCTATTGGCAGT







GGAATTGCTGGGTCAAAAGGTGACTATTTAACCTTTTGAGAAACT







GTGACTTTTCCAGAATGGCTGTACCATTTTACATTTCTACCACCA







ATGTATAAGGGTTCCAGTTTCTCCATATCCACACCAGCACTTATT







ATTTATCTTTTTGATTATAGTCATTCTAGGGGGTGTGAACTGGTG







TCTTTTAACATGCTTATTGGCCATTTGTAGATCTTTGAAGAACTG







TCTATTCCAATCCTTTACCCATTTTCAAATTGTGTTAGGTGTCCT







TTCATTATTGAGTTGTAAGTTTTTAATATATTCTCACTGCAATTC







CCTTATCAGATATCCTGATTTTTTTTTTTTTTTTTTTTTTTTTGA







GACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NUP62. In some embodiments, the sequences from gene NUP62 comprise or consist of:











(SEQ ID NO: 451)



AAACCCACCTTGTCCCATCCACATCAGGACATCCCAGCTGGAGTT







CAACCTTCATCCCTTCTGTGGCAGTTAGGAGACTGAATCAAGGTC







CAGAGAAGGTGGAGGAATCCTGATACTGAGCGGTACACACAAGCT







TCTTTCTCCTTCCTTACCTGTTGCGGGCAGGAAGTCCTTCTTGGG







ATCTGACCACCTTCCCTTAGAGCACTTACTGTCCACTCTGCCAGT







TTTTGAGCACTTGCCCTATATTTTCTTGGGCCTATAGGGGCTGTG







TTATCACTGAGGCAGTATACAGAGTAGCAGCTAAGAGCATAGACT







GAGCTTGTCCCACCCGTGGGCCTGCATGCGGCCTAGAACAGCTTT







GAATGCAGCCTAACACAAATTTGTAAACCTTACCAAAACATGCGA







ATTTTTTGCGATTTTTTTTTAAGTTCATCAGCTATCAATAGTGTA







TGTTATGTGTGGCCTAAGACAATTCTTCAATGTGGCCCCAGGAAG







CCAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene NUP62. In some embodiments, the sequences from gene NUP62 comprise or consist of:











(SEQ ID NO: 452)



AGACGGGGTTTCACTGTGTTAGCCAGGATGGTCTCAATTTCCTGG







CCTCGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACA







GGCGTGAGCCATCGCTCCCGGCCTCAGCATATTTTATTAATAGGA







ACTCTGGGGCCCAGTCTCATTTCTTCCTTACCACTTGTAAGAGCC







GCAGACGTTGGACTGTCCAACACCTGCTGTCATTCTTCTGTCTCC







CTCATCCCTCTAAAGTGTCTGTTTCCAGCTATCCTCATCTTCCAT







TCCAAATGATGGGTGCCCAGGCCCCATCCCCTGGCAGGGATTCAG







ACATCGAGGCCCCAGCTGCGTCCTTTTCTCCATCCCTTGCCCTTG







GCTTCCTTTCAAACCGTCCTTCAGCTTCCACTGGATAGGAACTAT







CTCCCAACCCTGCCAAAAATGGAAGATCTCCGTTTTCGTTTAGTC







ACAGGGTAGCCATCTTGCCCCTTCTCATGCACTGGGCCCTCCTCC







ACCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ATF5. In some embodiments, the sequences from gene ATF5 comprise or consist of:











(SEQ ID NO: 453)



GGCCCCAGAGTTCCTATTAATAAAATATGCTGAGGCCGGGAGCGA







TGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGC







GGATCACGAGGCCAGGAAATTGAGACCATCCTGGCTAACACAGTG







AAACCCCGTCTCTACTAAAAATACAAAAAATTAGCCGGGCGTGGT







GGCGGGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAG







AATGGCGTGAACCTGGGAGGCGGAGTTTGCAGTGAGCCGAGATCG







CGCCACTGCACTCCAGCCTGGGCGACAGAGACGGAGAGACTCCGT







CTCAAAAAAAAAAAACAGTTGAACAGGAATGTATGAGTTGAGCTC







TGTGCATAAGGCTTGCCAGACATCGTCTTATTTGAGTCTCATAAA







CCCACCAGGCGAGTTGATTTTTCTTATTTTACAGATAGGGAAATG







AAGTAAGAGAACTTAAGTTATTTGCCCGAGGCCATCCTGGCAGGT







CTGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ATF5. In some embodiments, the sequences from gene ATF5 comprise or consist of:











(SEQ ID NO: 454)



TCGGATCCCCAGCTGAGAGGGAGGAGGGTCCCGGACCCTAGGAGT







GGGAAGGAAAGGCTCGGATCCCCTGATCCCCAGGAGGAGGGGACC







CGGCTGCCTCCCGGTTGGGGCCGCGCGAGGGCGGGGCGCGGAAGG







ATCCGGGAGGGCCGTGCTCCGCCACCCAGTATATATCTGTCCCCA







GTCCCCGGGGCCGCCTCATTCCCTGTCCTCGGATCACAGTCTCTT







CTCACTACAGTGTCGCCGCCTCTGCCTGCGTAGCCCCGGCCATGG







CTCTGTAGCCTCGACCCCTTTGTGCCCCCGGCCCGTCTCCGCGCT







CACCACGCCTGCGCTCTCCGCTCCCACCTTCTTTCTTCAGCCGAG







GCCGCCGCCGCCTCTCCTTGCTGCAGCCATGGAGTGAGTAACCGC







TTACCTCTTCTCCAACTGCCTTTCCCGCCCTTTCGCAGCTCGTGG







CCCTCTTTAGGGAGCCCTGGGGGGTGGGGAGTCGCGAGCGGGGAA







TCAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PTPRA. In some embodiments, the sequences from gene PTPRA comprise or consist of:











(SEQ ID NO: 455)



GTTTTCTTACTTTGTCAATTTCTAATTTTATTGCATTGGGACAAA







AGAATATTGTACTCTTTCTACTGTTGGGGTTTATAAGGGCTGTGG







ATATTTCACTCGCCTTTGAAAAGAAGGTTTTCTCTGTTAGTCTGT







AGAGTTTGGTATGTACCAATTAGATTTTATTACTTATCATTTTGG







TCTTTTGTATCCTTACTTAATTTTGTCCTCTTGAATTTTAATGGA







GCAAAAGACATAAAGTCCTCTAATAACATGCGTTCTGTTTGCATT







CTCATACTTTTTATGAATATTGATGCTGCACTATTTGTGTACCCA







GGGAGAAGGCCAGACCACTGTCCAAAGTTTAGTGAATCTGGGCAG







CCTTGTTTCCCAGTTGTTGGAGGATGCCTCATGGAGGAAAGCATT







CCTAATCCTGGAGCTTGTTTTGTTGTACTCTAATTGAATTGTAAT







GTGTTTCTTTAACCTGAATGAATGTTTCTATTTTTTACTTATTAC







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PTPRA. In some embodiments, the sequences from gene PTPRA comprise or consist of:











(SEQ ID NO: 456)



TGAGCTGCTCACCTTATATCTGTTGTTCCTTTTACACAGTGTACA







GTATTCATTTATTTCCTCTGCTCACAGTCTGTGGTAACCGTGTGC







ATCTGTGGCTGTGTTGTTTGTTTACTTTCCCTTAAGTTATTTCCA







TGTTAATCTCATGGAGAAGAGCAATAGAAACAAGTACTGTATTCA







GTATGTTTTTTAATATAGACTATGGATTCTAACAGCTATGATGTA







TTTTAACAAGTAACAAAATATATCTTACTTTGACATGTCACTTTG







TTAACATTACTTTTTGGTGATATTAGGTCATAATTTCTATACCAT







TAGTTACTTCTGATTTCTAGGCCACAGTTCCCTTTAAATATTCTT







TGTGTTGTTTTTCCCCTAGTGTATAAAATGTCAACCCTTTGTGGC







TTTATATGGATTTTATGGATTTTCAGCCCTTAAATGTAAAGTCTC







TATGGCCTGAGATGTTGTGTCTGTGGTTTAAGCTGGACTGCTGAG







TCCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TPX2. In some embodiments, the sequences from gene TPX2 comprise or consist of:











(SEQ ID NO: 457)



AAAGAACAGACCTTCTTAATTCGAATCCCATCTTCTCCATTTATT







ATGTGATTTTGGACATGTTTGTTCAACTTCTCCTAGACTTTGTTT







TCTTTTTACGACATGGGGATCATAAAGCCGGGATGCAGTTGGGAA







TAAAACCGGATAAAGCTCCCAACGAACCGCGGGTTTCATAAGATC







AGGGCAGGGACTGGGTAAAAGGTTTCAAAGACTCCTGCACTATTG







GGAAGGACGCTACGGGCGAGTCTATGTTGCATGCAGGCGACTCCA







TGTTAAGACGAAGTGAACTCCCTGAACCTTCAAGGGATAATGCAC







AACCATAACGAGAATAATTTTTAAAAATAGAGTTTTCTTCCCTGA







AAACTTGGAGAGGTCCAGGATGCGCGCCATTCTGTGATCTAGGTT







GACTCTCCACCTCTTCAGTTGGTACCACCTTTCCCAAATAGTTTG







GAGCAGAGCACTCCGGTCTTTGTAATAACGTACTCCTAAGAGCTG







TTCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TPX2. In some embodiments, the sequences from gene TPX2 comprise or consist of:











(SEQ ID NO: 458)



TAACGATAATTATTGTTATTACCATGCTAACAGGTTATAAACATT







GACTGAACTAAGCACCCTGCATGCCCGTTCTCATTTAATTTCCTC







GCCAAGCCAATGTAGTTGGTGCTATTCTTGGTCCCTCTTGACAGA







AACTGAGGAAGGAGGAAGGAGGAGGAAACTGAGGCCCAGAATCTA







GATAAATTTCGAGAAATGGGTGATACAGTAGCCATGCCCTCAGGA







AGTGTGGCGTTTGATGGAGGAAAACAGAGGTAGTCACAAATAAGT







ACATTTCAAAACTGTTGTCTAAAACAGGAAGATAATTAGATGGTG







TGAGAACATAATTGAGGGAGAGTTTAAGTCCACTTGGAGTTGTCT







TCTATTTGGGATTTAAAATTCATTATATGTTATCCATATTTGTCC







AGCTCTCTTTTTGTATGGAATCACCAGATATTATTCCCTTTCTCA







TTTCCTTTCTCAACATTTATAGATTGGAGGGGGGAAGGAAAACCA







AACC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CBFA2T2. In some embodiments, the sequences from gene CBFA2T2 comprise or consist of:











(SEQ ID NO: 459)



CAAGTCTGGATCTGAGAGGCAGCTTGTGTGAATCATTTACATACA







TTTCTGTTTATATTCCCCAAATTAATTTGCAACTTTATATTTTTC







ATCCAATTATATGTTCAAAACATATTTTTAATAATTGATTTTAAG







AAGGAATTTGTTATATTAGGATTTAAAAACTGGCTCAAAAAACAG







AAGCTATTAAACAATAGCAGAAACCAGACAGTATATAGGACTTTA







AATGGTAATTGCTAAACATTTGAATGTTCCCAAAAATGTGAGTCT







GTGTGTCTCTGTGTGTCTCTACGTGTTATTCCACTAGGGATTTGC







ACAGATAGGTTTTGCACAATATCCTTGCTGTTATTGTCAGCCATA







AACAAATTACACGTATTTAACAAGTTAATGTCAGATTGGCCAGGA







GCCACTGGGAGGTGTGTACTTAGTGACATCAGCTGGCAAAGAGCC







CTGGTAACAGGGAGGGTTGGAAGGGTAGTGGAGAGAGAGGAAGCA







GGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CBFA2T2. In some embodiments, the sequences from gene CBFA2T2 comprise or consist of:











(SEQ ID NO: 460)



TAAGTACATTTTAATATAGTTTTGGGGGTTAAAGTGCACAATTCT







CTGCCTTTCAAAGCTTTTTACTTCTTTCAAATTACGATGATATGA







ATGTTTCTTGGTATTTGATACAAGAGTTCAGCCTTTCTACTCAGT







TTTATTTTAATGCTTCATTTTATTTATGTTTCTATTTTTCTGTTG







CCAACCTTATATTTGAATGTCATGCTTTTTTGAATGCAAGGTTTA







CTTAATAATTAGATGTAGTATATGAATTCTACAGCTAGAAAAGTA







GTCCCACAAATATTTCTGTAAGGTTTTCCACGTTGGATTGTTCTG







TGATGTTTAGAGGGAATTTTTTTCCCCTTTAGTATGCATAATACA







AATAGGCCATATTAAAAAGATGGCTCATAAAACGCTCTTTTCTAT







TGTCAGATTTTATTTCAGTATATGCAGGCAAATTATAACTAAAAG







AGGTTGAAGCAAAACTAACAGCATCTCTTCGTTTTAGATATATCT







AACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DYNLRB1. In some embodiments, the sequences from gene DYNLRB1 comprise or consist of:











(SEQ ID NO: 461)



AGCAGAGTTCTCGCAATTCCTACTTAAGCAGAATGAAAGCCATCA







AGGGCAACAAAGTGGAGGTGCTGTGTCCTTGGATAGAATGTTGAT







TTCCAGCCAGGCGTGGTGGCTCACGCCTGTAATCTCAGCCCTTTG







GGAGGCCGAGGCGGGCGTATCACCTGAGGTCAGGAGTTCGAGACC







AGCCTGGCCAACATGGTGAAACCTTGTCTCTACTAAAAATACAAA







AATTAGCCTGGTGTGGTGGCGGGCACCTGTAATCCCAGCTATTCG







GGAGGCTGAGGCAGGAGAATCGCTTGAACCAAGGAGGCAGAGGTT







GCAATGAGCCGAGATCACACCACTGCACTCCAGCCTGGACGACAA







GAATGAAACTCGGTCTCAAAAAAAAAAAAAAAATGTTGATTTCCA







AGTCAGCAGAGCCCTGGCAAGCACAGAGCTAGACAGAACGTGCTC







CCCAGCCCTGTCTAGTTCTGATTAAACCATGTGGTCATTTCATTT







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DYNLRB1. In some embodiments, the sequences from gene DYNLRB1 comprise or consist of:











(SEQ ID NO: 462)



TGAGTGCTGCTTTTTTGTATACTCATGGGGCTGATTGCACCATAG







CTGAGAACGTGGAATGCAGAATGAGCCCACCTGGCTTTGAATCTG







GGCCCTACTTAACTTATCAGCTATGTGGTTTTGGGCATAAGCCAC







TTTCTCTGTTAAGTTTTCTCATTTGTAAAATGTGGGTCATAATTC







TGCCTTCCTCTAGGGGAAGCTTTTAGAATAGTGCCTGGCATGAAA







TGCCTATGAATGTCTCAGTTAGTGTGTGTGTGTCTTTAACTTGCG







TCCCATTCTCAGGGCCCCCACATCAGTGTCTCGTTTACATCTCAC







ACAGTGACCCTGCGAGGCAAGGGTTTGCCCAGTTTGCAGATGAGA







AACCCAAAGCTGAGAGAGGTGTGGGGAACCGCCCAAGGTCACCCA







GCTGTGAATGAACGTGGTCAGAACACAGAATCTGAGTTGGTCACA







CTTCCCACTGATCCATGGGGCCTTTAAGCCCTCTGGAAGCTTCCA







TTAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ERGIC3. In some embodiments, the sequences from gene ERGIC3 comprise or consist of:











(SEQ ID NO: 463)



CGGGGGTGCTCTGTATTTGGAAGGATGTATAAATGCAAAGGTACA







GTTGCCCAAGGCCAGAGATCTGGCTGAATTGAGGCCTGGGAGTGA







GTTTCTAACATCCTTGTTGATTGGGGCTGGGGTGGGTAATAATCT







CTTACAGTGTAAAGTTTACAAGTATTTTTCCATCTTAGATCCCAC







GTGATCCTTAGGACAGCCCTTTGCAAAGGAGAACACTGAGGCCCA







GAAAGGGCAACTGATTTGCCCAAGGCCACACAGTCAGGAACTTGG







CTCTTTGAGCTCTAGGCCTCTTGTCCTTCTAGGCCACTATTTTGC







TCCTCTCTGGCCAGATTAGTTCACTCTCGAGGAGGGGCCCAGAAG







GAGGCTTGTTAGCTGGGGCCAGACTGTGTTGCTGAGGCTGAATGT







TCCAGTCTTCCCTCCTGCAACATATCTTGCTCTCATAGTTCTCAT







TGTGAAGCTCTGAATCTTTCTTCTCTCTCCTCCCTCCCCCATGCT







GCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ERGIC3. In some embodiments, the sequences from gene ERGIC3 comprise or consist of:











(SEQ ID NO: 464)



TAAGTGGCCCTGCCCCCATGGAGGCCAAACCCCTACTTCTGGGGC







AGCCTGCCCTACTAGAATGGCGGGGAGGTTGGATGGGGCTGTGAT







TGGGTCTCCTCTGACCTGGCTGCTGGGTGACTGTAAAAGGTCTGG







GATGTATCTTTGAAGTATGGTTCCTTGCAGCAGGAGGGATTGAGG







CTAGAGGGCAGGAATAAATTGTTCTGGAACAGTTGGTACAGGGTC







CCCATGGGATTTCTTCTGGGGAGCTTTGAGGGTGGAGGCAGGGAA







AGTGTGGATGGCATTCTCAGATCCCACCTCACTTTGAGGTTCAGT







GATTTGCCATCAGCTCTAGTTAGTGAGAGCCCATAGTCGGTGGGT







TTTGTTTCTGGAGATGCAGGCAGGGCCATGGTGGTAGTCAAGGCA







TAGAGTTCCCTGTGGACGTCAGGGAGGAACCAGGGTCTGTTCTAA







AGACTCCTGAGGAGGGCGTGAACTCTCACACAGGCCCAAGGCTTG







CCCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DLGAP4. In some embodiments, the sequences from gene DLGAP4 comprise or consist of:











(SEQ ID NO: 465)



GGCACCCATTCCTAGAGGCAGGAGCCCCAAGTTCTCGGGGGTGGG







GTGGTTCCTGACTGGCGCGGTGTGACCCGGGCCAGTCTCGTTCTC







TCTGGGCCTCAGTTTCCCTCCTCCACGGGGCGGCGATGGTCAACA







CAGGTTCCTGCCGGCCCGCCCCGTAGCCTGGGGTTCCAGGCTCCC







GTGCCACCGCGGCCCGCGCTGTGTGACCTTGTTCCAGTAGCCTGC







CCTCTCTGGACTGGCGGCCCCGGATTTCCAAGCGCTCTTCCGGAT







CGCGACAGAGCGGTCGCCCGCTGCGGAGACCCTCCCCCTTCCCCC







GGGTTGCGGGGGCGTGGGTGGGGGTGGGGCGCCAGGAACGGCGCG







GGCCCCCCCAAGGCCCCCCCAGCCCCGCGCCCGCGGCCCGATCCG







CGCCTGGTTTTGGGCGCAGCCGCACGGCCCCCTCCCCCGCGCTCC







TCTCCCCTCTCCCCGCCCCCTCCCCGCCGCTCTCGGGGAGCCGGG







GCCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DLGAP4. In some embodiments, the sequences from gene DLGAP4 comprise or consist of:











(SEQ ID NO: 466)



TGAGGGCGCGGGGGTGGCGCGCGGCGGCTCCTACCCCCTTCCCGG







GCCGGCGCGCTCCGTCCCTCGGCTAGACCCCCCGATCTGGTCTCC







TCCCCGACAGACCTCCCTGAACCGGGGGACGGCGCTGGCGCAGAA







AAGCCCCCTCCCCTGCCAGTTCTGTCTTCCGATCCCGGGTCCGAG







GGGCCGGCTGTGCGGCTGGGAAGCGCCTTCTCCGGTTGGGATCGC







CCCATCCATGTCTCCCCGGACCCTCAAATCGGGGGCGGCGGCACC







GGGGCCCGGAACCCCTGTCCGGGACCGGATCCCCATTCCGGCTCT







TTTTCCCGCGGACTCCCCCGTACCCCATATCAAGCGGCTGCCAAA







GCCTGGAAGCCCCCTCCTCAGGCCCGCCCCCTAATCCGCAGGGCG







GCCTGGGGGTCAGGCGGACCCCTCCCGTGCCCGGCCAACCTTGGC







GCTTGGGAGAGCAGGGCTATGCCGGCCCGGGCATTCCTCCATTCA







ACCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SOGA1. In some embodiments, the sequences from gene SOGA1 comprise or consist of:











(SEQ ID NO: 467)



GTGAGGCCCACCCCTGCCAAGCCGCTTCCACCCGCAAGGGAGAGT







TGCTACAGAAATGATTCAGAAGCAGGAACTCCTTCAGGTATAGCT







GGATCTAGGTGCTCATCCAGTGAGGTCTGGCACACCATCTCCTTC







AGGTATAGCTGGATCCAGGCACTTAGAGAATGCAGTGGGAACTAG







ATTTTTCTTTCTGCTTCAGCCTGTCAGGCCTGCTTTCCTCTGTGT







TAGCTTTACTCTTAAACAAGCTATTCCCAAATATGGCCACTGTTA







GTTTCATGCTCATATCTTACAGCTTAGCAAGTACAGTGGATTGGC







CAGATATGAGTCATGTGCCCACCCCTGGAGCCTACCCAAACCCAG







GAACCAAGAGGGAGGAACTAAGAGTGGTTCCCATGGGAAAATTGG







GAGGCCTCCAATCAAAAGAAGGAGGAAAGGAAAAGCAATCATTGA







CCCTCACTCCCCATTATAGTTCACTTCATGCAGCCAGGCACCACC







TACC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SOGA1. In some embodiments, the sequences from gene SOGA1 comprise or consist of:











(SEQ ID NO: 468)



CCTAACCCTCTTCCAGATCTCACAGCTCCCTCCATCTGCATATTC







CACGGTGTCCCCTGCACCTGCTGCCCCCAAAGACCTGGGCATTTC







AGGTGCTCCTCCCACCAAGAACCTGACCAGCTCATGGGGGTTAAT







GGGGGGCATTGAGTCTAAAGGTTGCACATTCCCTTTCCTGCCACT







CTGAGGTTTGAGGCCAAGAAAACCATCTTGCTGGAATGACCACTA







GTAATCTTTCTGAGTTTTTATGAGGATACTGACATCATGCCTGAA







AAGCCCACAGAAGAGCATGGCTTATAGAAAGAGCTCTTCAAACCC







TTATTGTGTGGAGGGGTTAGGGGTTAGTTCTGAGTGGGGAGGACT







AAGCTGGGTTAGTCCCTAGTGAGGGGTCTTCTGAGGGGACCTCTC







CCCTTTTCCCCGCTGGCACCCAGGAGGGAAGGAGAGAGAAGGGCC







AAGAGGAGTGGCCTCCTGCTGCCAGCTGCTTAGTCTGCTTCTTTC







CGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DBNDD2. In some embodiments, the sequences from gene DBNDD2 comprise or consist of:











(SEQ ID NO: 469)



CCTTAGGGGCAGAGCCTAGTCTGGAAGTCAGGCCTGTGACTTCAG







AGCAAGGGTTTGGGGTAGATGGCACATGCGTGTGTACGTGTTTGT







GTGGGTGGAGAGAAAAGGAGGGGCGCGGGAGGAGTTGCTTTAGCC







TTTGTCTGGGTTTTGAAGGATGGGAAGGGGTGGGCTGCAGCACTG







GAGGAAGGGAACCCTCCACCCTGAGATCTCTGTCTCTATCCTATC







CTGTCCCTGGCCTTCTGAGGCAAGCGGGGCCAATTAAGGGGAAAA







CGTACCTCCTCCATTTGTGCTGAACCAATCCCTCCAACCCCTCTC







AGGAGGGCATGATATGGAGAGTTGGGCATTGGCTGTGTTCCCTGA







ATACAGAGTATCTCTCTTGTGGTGCCTGGAACTGGCATCCCCTTT







GTGGAGCTTAGGGCAAGCCCCGCCTCTGCATGAGACTTGGTTTGT







GGGACACACTTGGTTTCAGGGAAGGGGAAAGAGGTCACCAAGGGC







AGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene DBNDD2. In some embodiments, the sequences from gene DBNDD2 comprise or consist of:











(SEQ ID NO: 470)



AGCTGACATGGACCCAAATCCTCGGGCCGCCCTGGAGCGCCAGCA







GCTCCGCCTTCGGGAGCGGCAAAAATTCTTCGAGGACATTTTACA







GCCAGAGACAGAGTTTGTCTTTCCTCTGTCCCATCTGCATCTCGA







GTCGCAGAGACGTAAGTCCCAAGTCCTGAGAAGAGGGACTGGGGT







AGGGTAGGGAGGATGTCCTGTGGGTCCTGAATCTTGTGGCACTCT







CTCCCCTCTGGTTTTCTTGGCCCTCTATGCTTCTAACTTGGGACC







TGACATGTAACTCTCACTGTCCTGGTGTGCAGCTTGGGTCCTCCT







GACTTGCCCACTTCTTGATCCGCAGCCCCCATAGGTAGTATCTCA







TCCATGGAAGTGAATGTGGACACACTGGAGCAAGTAGAACTTATT







GACCTTGGGGACCCGGATGCAGCAGATGTGTTCTTGCCTTGCGAA







GATCCTCCACCAACCCCCCAGTCGTCTGGTATGCCCCTCTGCTTT







GGGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TPD52L2. In some embodiments, the sequences from gene TPD52L2 comprise or consist of:











(SEQ ID NO: 471)



AAGGGTGTCCCACATCCTCCCACGGTGCGTGTGCTCCCTCAGCTG







ACGTGATTTGGGGAACACGGGCCGCTCTCGTTCTGTGGAGCTGGC







CAAGCCCAGCTGTCATGCTGTGGCACATTTCACACCTTTGCGCTG







CTGGATGGGGCTGGTTGTGCTCCAGGTGCTGTGGCTTCTCCAAAC







AGGTGTGCCTTGGGGCATAAGGCGTGGACAGGGCGTGGACCCAGG







GGCCTCTTGGGCACAGCCGTGAGGCTGAGCAGTTGCTAGTCATGA







TCTTGCGAGAGCCTCCTGTTGAGTCAGGACTTGAGGAGACTGGAG







ACAGTGCTGCTGTCCCCGGTGGTGTGCGGACTGGAGGAGCCGTCC







TGGAGGGTCTTCCCCTGCTGGGCCTGACTGAGCACGAGCAGGGGG







CCTCCCCTGGGGCTCCCCGAGGAGGGCTGTGAGTGGGTGCTGGCC







TCCCTTGCTTACACTTCGTTTCCTCTTCTCTCCTGCTGCCACCTT







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TPD52L2. In some embodiments, the sequences from gene TPD52L2 comprise or consist of:











(SEQ ID NO: 472)



TAAGGCTGAGCCTGGACACACCTGTGGAAGACTTTTCTCTGAATT







GGGGCAGGGTGGACTCCGGCAGGGCCCTTGGGCGGCTGTGCTAGT







AGAGGGCAGTGAAAGTGGGATCACCCCTTCCAGGACAGCAGTGCC







AGCCAGGTGGTTGCTGCATCTCAGGGAACAAAGCAGCAGCGTGGG







ATCGACGCAGAGGCCCCCCGGAACCCCCCGGCCCTTCTGTGTCCC







CTCCTCGTGCTTCCCTTCAGGCTCACAGACCGCCTGCTGCCGGGT







CTGTGAATAACTGGCCTGGCGTCCAGGCCTGAGTTTACTCCTGAA







AAGGAGCTTTTTTTTTTTTTTAGACGGAGTCTTGCTCTGTCGCCC







AGGTTGGAGTGCCGTGGCGCGATCTCGGCTCACTGCAAGCTCCGC







CTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCTCCGAGTAGC







TGGGACTACAGGCGCCCGCCGCCACGCCCGGCTAATTTTTTGTAT







TTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CCT8. In some embodiments, the sequences from gene CCT8 comprise or consist of:











(SEQ ID NO: 473)



GTGCGTTTTGCCTCCATCCGTAAATGGGGCTAAGGCCCGTCACCC







TTAAAGGAGGTTGTGAGGGTGAAATTGAATAACGTAGATGAAATT







GTCTTGAGAACTGCGACGTCGATTATCACATAGCTCGCGAGTTGT







AGGATGGGGAAGAACGAGAACTAGCCGATCCAGAGAAGAGAGTGG







GAAAAAGGGCCGGGTCTTGGTTGCTTGCTTCCCAGTGAGAAACAT







ACGGCTTTCAGCTTAGTTGACAGAAGCCATGCGTTGTAGCCAAAT







GAGTTCCGGTCCCAACTTATGTTAGCTGGCTTAATCTAAAATGTA







ACTTTCTTGGGTTTTGTTTCCTTCGTCTTTAAATTATGGAGATTG







GACCAAGAGGTGGTGTCTTTGAGTTCTGTGTAATCTGCAGGATAA







GTGATAGAGGGGTGCAGATAACACTTTTGTTTAATGGCCTTTTCT







TGAACGTTAGTTCGGTAGCATAGTTTTAAAAAATATCTTACTGCA







CTAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CCT8. In some embodiments, the sequences from gene CCT8 comprise or consist of:











(SEQ ID NO: 474)



TGACGTTGGTGGCTGGGTCTGGAAGATACGTGGAAATTAAGCTAA







GGATGTGTGGCTTCCAGATCAAAAACCGCAAAAATCTAACGCCGT







GACTACTGACTACGGTCAGAGAGCACAGACTGGAGCAACCTCTCA







CGGCCTGGGCTGTCTGCGCGTGCGTGAGCCAGAAACCCGAGGGGC







TCCCTGGGCCCGCCCTATCGATCGACCCGATCGGGGATCGTCAGC







TTGGTTCTGGCCACAGAGGTTGCTCTTCTCGCGATGCTTCAGACC







TGGCGGCAGGGAAAGGGTGGGCTAATTGGAGAGCCAGGAAGAGCG







TGAGGCGGCCCCACGCTGCTTTCCCAGAAGGCTGTGCGTGCTCCT







CGCTTCCTCCGCGGTCTTCCGAGCGGTCGCGTGAACTGCTTCCTG







CAGGCTGGCCATGGCGCTTCACGTTCCCAAGGCTCCGGGCTTTGC







CCAGATGCTCAAGGAGGGAGCGAAAGTAAGGGCTGAAGGAAAGGA







ATGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TMPRSS3. In some embodiments, the sequences from gene TMPRSS3 comprise or consist of:











(SEQ ID NO: 475)



GTACATCTGGGTCTCTATGTGGTTCTGCAGCTCTTCCTTTGTTTC







AAGAGGATTTGCAATTGCTCATTGAAGCATTCTTATGATGGCTGC







TTTATAATCCTTGTCAGATATTAATAATTCCAACTCCTGATTCAT







GTTGGTGTTGGCATCAGTTGATTATCTTTTCTCATTAAAATTGTG







ATGCTCCTAGCTCTTGGTATGACAAGTGATTTTCAATTGTATCCT







GGACAATGTGGCTCTCATGTTTGCAGACTTGAGTCCTACCTACAT







TTTCAATGTTACCCTGTTTAAATGTAGTTTGTAGATCCTGGCCTA







CAGAATGAATGCACTGATGACTCTGCCAGGTAGATTGGTGGGCTG







TAGTTCCAACAATAATTTTAGAGCCTTCGTGGGCTGTGTGCTTTG







TCTGGTGCCACCTTCTCCCTGTTGGTGCCACCTTCTCCCTGTTGG







TGCCACCTGGAGGCAGAAAGAGCTTCTTGAGGCCAGACCAGCTGG







GGCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene TMPRSS3. In some embodiments, the sequences from gene TMPRSS3 comprise or consist of:











(SEQ ID NO: 476)



CATAGCTAATTCCAGTGGACCATGTTATGGCAGAGACAGGGTGAG







ACGGTGGTGCGGGAGGCTAGAGGGAATATGTTGGGGGCCAGGTAC







TGGGTCTCTTCTTAAAATCTCAGGAGTTTAGAGTGCAAGAAAACT







ACAGAAAAGACTTCCAAACACAATGGTTCTCCCTGATATTTTTAC







CCCCTATTTTGTTGCTGTTTGATGAACAGTAGTGCCTGATAGACT







CCTTGCAGTGACCAATGTTGAGTTCAGCCCTCAAATGCCAAGACG







ACCTCAGAGGTGCTGAGAGTGAGGTCTGGGAAACACATGGGGTGT







GGGTCTGTGTTTCTGTAGGGCGTGGCAGTGCAGTTGCATATACAG







CATAAACAAGCAGGGTGATGGGACCACATCTTGCCTGATAACCTC







TCCCACCATCTTCCTAGGTTTTCAGGATACCTGAGGTCAATGAGT







TCAGTGGTTGGACTTTTCCTGTGCTTCACCTCTTGCTCTTCTCAT







TTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PFKL. In some embodiments, the sequences from gene PFKL comprise or consist of:











(SEQ ID NO: 477)



CGGCTGTCTTTCTGGGGGAGCTTGTCTGTCTGTCTGGGGGGCTTG







GCTTTCTGGGTGTGTGTGGGATGCTTGCTGGGTTCCCGCCCCAGT







TTCAGCTGTAGCAGCCGTGCTCCCATCTATGTGGGGATGTGAGGG







AGGCACTGTTCCTTTAGAGACGGCGTTGCTTGCTCAAGTGGAGAA







GCTGCCAGTGTGGGCAGCTCCAGCTCCCTGCACGCTCTGTGCTCT







GCCCATTCTAACGTCTGTGCCCCTTCTTCTGGATGGCTGCCGGGC







TTGGCTCTGGCCCCCCTGTTGGATCCCCTGGCTGCTGGGTGGGGA







GAGGGCCACCTACTGAACCTGAGGGTGGGTCTGGGTGAGTCCTGG







CTCTGCTGCCACCTGCTGGGCTCTGGAAGGTCCCTCACCCTCCCT







CTGGGATCTCATTGGATTCCTGTAGTGTGGGGATGGTGGCAGATA







CTCTGGCTGCTACGTGAGAACAGTGTGCCGATCCCTGGGGTTTCT







CAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene PFKL. In some embodiments, the sequences from gene PFKL comprise or consist of:











(SEQ ID NO: 478)



TCAGCACCTCCAGCCTCTGCAAGTACAGGGGGGTGGGAGGGCAGG







GGCTTTTGAGGGGCACGAGGCTTGGGGACAGAGGACCCGCATGGC







TTAGGGATGGATGGCATGGTATGGGTGCTGGGAGGGAGGCAGGAA







GAGCAGGGCTGGTCCCAGGGGCAAGCACAAACTCAGGCCTGTTAC







ACGGCCCCAGACCTCACTCAGAAGGGGACCCTGGCCGCTGGCGTG







GGTTTCCTCCTCTCTGAAGATTAGGTGGTCTTGGCTGCCCCTTTT







GTATGAAGTCATGGATATCTTTTGAGATGGGGGCTCTCGGGAAGA







AGGTGTGGGGTACATTAGCACCAGCGTTTCCTGAGTGCCGCCTCC







CCCGTTAATGTCCCTCTCCAGGAAACTGGCTTTGCCAAGGCCCCC







GCTGGGACAGACTGTTTCTTTCACTGCAGTCCTGGGAGCCGAGGG







CAAGGGGACAGGAAAGAGGAAGTGACCTCAGAGCCTGGTGGCACC







AGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene S100B. In some embodiments, the sequences from gene S100B comprise or consist of:











(SEQ ID NO: 479)



GTGAGAAAGAGCCAGGCCAAGAGGACGCTCAGGAAGAAATGGTTC







TTTTCTTTTGGGTGGAACGGAATGGAGGGGTAGAAACTAAGTGGT







AGCTTAAAAAGCCCCTTTAGGACAATTGGCAGCATTTCAGAAGTG







TCAATAGGATGATGTGTTTTAATCTCCACATTACTGCTGCTTTGT







GGACACCTGACTGCATCAGCCCTAGACAGCTAGAGGTGTGTTTTG







CCATAAATCAGAGAAACGTCAGGTTTCATGGCCCAGAAGTGATTG







TTGACATTTTCCCAGCGGTACTACAAATACTGCCTCTTCTGTAAC







TATTTGAAGAGTAAAGATTTTGCTTCCCACTGGGGCTAAAAATGA







TGGAGAACCTAATGCTAGTTTAGTCCTATATCATAAATAAATGCT







TCCCTTTTCTCCTTATATTTCTTCCACCGCCCTGGGTAAGTAACT







CTTCAGTTTTCCAGTTTCCCTCAGTTTGAAGTGCCAGGGTCCCCA







CAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene S100B. In some embodiments, the sequences from gene S100B comprise or consist of:











(SEQ ID NO: 480)



AGGCAGCCCCGTTGAGCCCAGGGCTGACCACAGACAAGCACTACT







GTCCACAGAACTTCACGCCCAGTGGGGCCAGACTTGGAGGATGGC







AGAGGAGAGAAGCTCCAGGGGCCTCTCCATTAGAAACCAACTTGC







AGGCCACTTAATCTCCTATGCTCAGCCTGTACTTGGAAGCTGCTT







GTTCTTGGCTGCACATTTGCTTTGTTTGAATCAATTAATCCAAGT







CTCTCTCCTCTCCCCACCCCCAACCTTGCCTTTAGGGTGACATCA







ATATTCATGTAATAAAGAAATCGCACAAAAAGCTGACTCCCCACT







TCCTGCCCTACAGGCCCTTTTTTCTTCCTCAGCCCATGTGCAATC







CTGGCTCCCAGCAAGTCCCCGGGGCTGCTTGGTCAATGCAGCCCT







GTGTGCAGGCCTGGCAGCCCTGCCACCCCCGCCTTCGGCTCCCAT







TGGCTGCCACGGCCTGCAGTGGGCTGCACCAGGGTTCATCCATCC







TCCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RANBP1. In some embodiments, the sequences from gene RANBP1 comprise or consist of:











(SEQ ID NO: 481)



TACTGGTTTTGAATCGCGGCGCGTTTCCCGCCGCTGGGGTCAGGG







GTCGAGGTTCGGGTCGTGGGGCGGAGGGAAGAGCGGGCGGGCGGG







AGGCGCCGGCGCCAGACGCGGAGGGAAGGAGCTACGAGTAGCCGC







CGAGAGGCCGCGGAGCCAGCGACGACCGACCCAGCCGAGCCGCCG







CCGCCGCCGCGCCCCCATGGCGGCCGCCAAGGTGCCGCCGGGCCC







AAGCGGGGACAGGGTGGGCGGGCGGGGCCGGGGCCGTTATCAGCG







GCCACGTGGGCGGCCCGCGCCGCCGCCACCAACCTCCGCCGGCCT







GCAGGCTCGGCCGTCTGCTGGGCCTCGGTGGCGCGGAGTCGGGGC







GCGTGGGGTTTGGGGGCTCGAGGCCTGCGGAGTTGGGGCGTTTGG







GGGTGCAGGCTCTGCAGAGGCCTGGGGGCTGCAGGGCCCCCGCGT







GACCTTGGGGTGGGTTGGGGAGCGGCCGTCGCCACGCGGGACGGC







CCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene RANBP1. In some embodiments, the sequences from gene RANBP1 comprise or consist of:











(SEQ ID NO: 482)



CATGTGTTCTGGAAGACTCGAACCTAAAATATGATTATTGGTGCT







CTCTTATCCTCTAAATTGGGCCAGGATTTCATGACTTTCACCGGT







ACAATTTATCTAGTTATTAACTTAGTTTTCTGAACTGTGAAAAAA







TTGCTGGCAGAGCTGTGAATCCAGTGTAAATACTAGCAGTCCTGT







AGCGACTTTTTTCGTTTTACTAAGCAAGAGCACAACTATTTCCTA







ATTAGTCAAAACTGAAAGAGTTACATTTTGCTTTAAAGTAATATT







TCAAAATCTATTTCGACCCATCATTGTGTCCTTTGATCTTCACAC







AGCAGGTATCATCTGCATTTTATGGATGAGGAAGAAACTGATGCT







CAAAGAGATGAGGCAGTTTCTGCCAGGCCACTCTCTGCAAGTGTT







GGCGGCTTGTAGGGTCCCCCATCAGTCTGTGCACAGATCTGATTG







ATGTCTGTGAATCTGGGGGAGGGGCCCCAAGTTCTGGTGTCTCAC







TCTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene UBE2L3. In some embodiments, the sequences from gene UBE2L3 comprise or consist of:











(SEQ ID NO: 483)



CCTGCCTCAGCCTCCTGAGTAGCAGAGATTACAGGCGCCCGCCAC







CACGCCTGGCTAATTTTTTGTATTTTTAGTAGAGACCGAGTTTCA







CCACGTTGGCCAGGCTGGTCTCGAACTCGTGACCGCGTGATCCAC







CCGCCTCGGTCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCCCC







GCGCCTGGTCAACTATTTTATGCAATCAGTATTTATTATGCTCCT







ACTATGTGCCAGGCACTGGTTGTAACTGCCATCTGATTGGACGAT







CCGTAAACGCTGAGGCCCAGTCTGTGCGGTTCACTTGCGTTCCTC







CACGCGCCCCCCAGCACAGTGGGAAGCACACAGTAGGTGCTCCGC







TCTGCTCCTGTGCCCCGCCCCGCGGCCCCTCCCCCGCTCCAGGAA







GTGCGGGGGCTCCAGCCGCCCGGCCGGCCGCGATGCATTCTGGGG







AAGGAGCAGCACCAAATCCAAGATGGCGGCCAGCAGGAGGCTGAT







GAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene UBE2L3. In some embodiments, the sequences from gene UBE2L3 comprise or consist of:











(SEQ ID NO: 484)



TAAGTTCCAACTCCAGGAAGGCCTGAGGTCGGCCCCGATCGCGGC







GCGGTTCTGCTTCCCTCTCCTCCACTCAGTCATCCCGATCTCCCC







TCCCTGATAAAGTGCCATTTTTGGGGGGATAACGGTTGATTTCAG







TCGTTCGAATCAAAACGGGCCAAAGTTTATAATAATAATTTGGTA







CTTTGGATCTTGAATGCAGCAGGGTGCTTTCTCGCTAATTGGCAC







CTTTTGGTGAGGAACACATGCTAAATTGGCTTAGTACTTTTGCCA







GTGGATTTGCCAAGGTCCTCTGTAGCCCACCCCGCCAGCATCTGT







GAGTCGGCGGCTGGGTAGTGCTGCCGGCCTGCAACGGGCCGGAAA







TTAAAAAAAGGCCCAGGAGCCAGTTTCCTGAGGGGAGATAAAGAG







CTGGGGTTCGTTCTAGGCGGAGCAGTGCTTGGTAGAGCTAGACAT







TGAACTTGGAGCCACAGTCTCTGAGCATTCCCTCCGTCCCTGGGA







ACGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CSNK1E. In some embodiments, the sequences from gene CSNK1E comprise or consist of:











(SEQ ID NO: 485)



GTGATCTGAACCTGAAGTCCGAGGCCCCAAGAGTTCTAAGTAACC







CAGGGGTGGAGGTGGGGTGGGGGATCCCAGCTCTTTCCGATCTGG







GAGATCAGAAAGATGCTCATCTGGGAGAGTGGGACCTCCCCTTTC







AGTCCGCCCACCCCCGAGGACCGCACTTGGAGGGCCGCACTTGGA







GGACCGCGCCTCCAAGGCGGGCGCGGCTGACGCCCGGGGCCCAGG







CCATGTGGGGCGTGCGTGCCGGGAGAAGTGTCCCAGCGCCTCACC







CCGCAAGCATGAGAACCACGAAACCCCCGGGGCCGGGAAACCGGG







GCGGGGCGGGGCGGGGCGGGGCCGGGAAGCGGCGCGGGGCTGGGC







CGGTGACGTCGCGGCCCCGCCCCCGGCCGGCTCCCTCCTCCCGGA







GCGCTCCCCAGCGGAGTCCTCACCTGCAGGCACAGGTGTGCGCAC







TTCGCATCTCGAAGGCCGGCCAGCAAGCTTCAGGTGAGCGCCAGC







AGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene CSNK1E. In some embodiments, the sequences from gene CSNK1E comprise or consist of:











(SEQ ID NO: 486)



GAGACCCCATCTCTACAAAAGATGGAAAAATTAGAAAGGTGTGGT







GCTCGCCTGTAGTCCCAGCTACTCAGGAGACTGAGGTGGGCAGAT







GGCTTGAGCCCAGGAGTTCGAGGCTGCAGTGAGCCGTGATCACAC







CACTGCACTCCAGCCTGGGCAACAGAGCAACACCCTCCCTGCTTC







AAAAAAAAAGTAAATAAAAAGATCTCCACAATGAACAGGTATTCT







GCATGACTTCCTTGGAAGAGATTTCCAGAAATGGAATTGCTGCCC







TAAGGCAAACGCTAATTTACGACTCTTGGGCAGCTGGAACCTGTC







CGACAGAGCGGAGACCCTTATCTCTGCCACCACCAGCAGGCTGAT







ATTTTCAAGAAAAAAATTTGCCAAATACAAGCAGTCTCAGATAAT







ACAAAGAAGATGGAAAGTTTCCGGAGCGCAGGTGTCAGATCCAAA







GATAACTTGACCCAAACACCCCGATCTCCATGTCCCTATCTCTCT







CTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SUN2. In some embodiments, the sequences from gene SUN2 comprise or consist of:











(SEQ ID NO: 487)



GCTACTCGGATGTGGACCAGCAGAGTTCCAGCTCGCGGCTCCGAA







GCGCCGTCTCACGGGCGGGCTCCTTACTCTGGATGGTGGCCACTT







CGCCAGGTGGGCTGCAACCCGTGCCCAGTTACTTCCATTCTTGGT







GATAGTGCTTTCTTACCCATCTCTCTGCAGTTGGGCATCCTGCCC







ATGCAGAGAGACTGTAGGACCATTGGGGAAAGGTCATTCTAGAAT







AATCACTGGGAGCCCAGAGCTGTGGGGACCCCCTTGCGTGGAAGG







GAAGGAGTAGCTGCAGAGCAGGACAGAGGGTGTGGGCAGGTGGGA







TCCAAGGACCTGGGTCCCGGTAGGGCAGGACAGAGGCATACTGGC







AGGTGGGGGTGGGGTAGGGGCAGGGAGGACGGCAGACGGGACAAA







GGAGCGGTGCCCCCAAACACCATATGGAGCCTTCGGCTTGATGGT







CATTCTTCCAGGCCGGCTCTTCAGACTTCTCTACTGGTGGGCTGG







CACC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene SUN2. In some embodiments, the sequences from gene SUN2 comprise or consist of:











(SEQ ID NO: 488)



CTCCCTCCTGAGCAGATCCGAGGCTGCTGGGAGGCCTCAGGAAGG







CTGGGCAGGGAGCCCCGGGGAGCTGGAGAGAGGGCAGCATGTGGC







AGGCACAGCGCCCCTCCCATGCCCGGCCCTGGGTGCGCCCCAGCC







TGGCCTGCGGTGGCCCCTGGTGCCCTGGGCTGTGACTGCCCTTCA







CTTGTCTGTCCTCCCCACCCCACGAGTCTATGGCAACCTAAGGCC







AACATCCTTGCATTCAGGCCCATTCCAGCACTTTCCTGTGGGGCT







CACACATGGTGGGTGCTCAGTCATTCTGAGTGGTGTGCATGTCAG







ACCCCAGCACCTAGTTCTGAACAGCAGCTTGAGGGGTGGCTGGTG







AGGAGAGGGATGCAAACAGGAAGCTGTCAGAAGAAACAGAATGTC







AAAGAGCTCCCATTTCATTCCTTACTGCACACCGGGCCTGTGTGG







GGCTGTGGGGATGTGAAGGAGGGTGGGTGACTTGTGGTCTTGGCT







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ATF4. In some embodiments, the sequences from gene ATF4 comprise or consist of:











(SEQ ID NO: 489)



GGACCCCGGGATCCCTCTCCCAGCAGCCGCCCATCTAGGGCCCTG







AGCCAATAAGAGCTCGAGATATATATGATTCACCGGGCCTGGGCC







AATCACCTTGACGCGCGCTCTTCGCCAGCACCTCGCGCGATAACC







TGGCGTCCTCGGCCTTCACAATAAAAACTCTTCGCCGGAAAACGA







CCTTTCCCCGCCCACTGCGCTGACACCGGAAGCGAGGCGTGTCTG







GGAGATCACTCCGCGCTCCGGCGGCGAAGGAAAGAACGGACTCTG







ATCATAGAAGCCTAGTAAAGTAGTACACCTCTCTCCTTTCGTGAG







GCCATAAGAACAAACTCCTTTTCTCGTCACAGCTACGCCCTGGGC







ATAAACGGTTGGGGCGTCAAAGGGAGGGAGGGAAGGGAGCGGGCG







GGAGGAGACGGTCACGTGGTCGCGGCGGAAGGATGCGTCTGTGCT







GCGTCCCCATAGAGACGAAGTCTATAAAGGGCCGGCGGGCGGCCA







CGGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene ATF4. In some embodiments, the sequences from gene ATF4 comprise or consist of:











(SEQ ID NO: 490)



TTCTACTTTGCCCGCCCACAGATGTAGTTTTCTCTGCGCGTGTGC







GTTTTCCCTCCTCCCCGCCCTCAGGGTCCACGGCCACCATGGCGT







ATTAGGGGCAGCAGTGCCTGCGGCAGCATTGGCCTTTGCAGCGGC







GGCAGCAGCACCAGGCTCTGCAGCGGCAACCCCCAGCGGCTTAAG







CCATGGCGTGAGTACCGGGGCGGGTCGTCCAGCTGTGCTCCTGGG







GCCGGCGCGGGTTTTGGATTGGTGGGGTGCGGCCTGGGGCCAGGG







CGGTGCCGCCAAGGGGGAAGCGATTTAACGAGCGCCCGGGACGCG







TGGTCTTTGCTTGGGTGTCCCCGAGACGCTCGCGTGCCTGGGATC







GGGAAAGCGTAGTCGGGTGCCCGGACTGCTTCCCCAGGAGCCCTA







CAGCCCTCGGACCCCGAGCCCCGCAAGGGTCCCAGGGGTCTTGGC







TGTTGCCCCACGAAACGTGGCAGGAACCAAGATGGCGGCGGCAGG







GCGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene KIAA0930. In some embodiments, the sequences from gene KIAA0930 comprise or consist of:











(SEQ ID NO: 491)



GTGGGGGTCGGGAGTGGTGAGGGAAGGGTGGCGCATCTCAGAGCC







CTCGCAAATTTCTGGCCAGAAATGTGGGGGTATGGGCGCAGCTTG







GCCAAGGCCATGCTGCAAGGGCCAGGGTGGCCGTCCTTCCTCACT







CCTGTCTGGCCTCCCTCTGCAGGGTGCTTCAAGGATGACCGCATC







GTCTTCTGGACTTGGATGTTCTCCACCTACTTCATGGAGAAATGG







GCTCCCCGGCAGGACGACATGCTTTTCTATGTGCGCCGGAAGCTG







GCGTACTCCGGCAGCGAAAGCGGTGCAGACGGGAGGAAGGTGAGT







GACCCCCGGCCCCTGCCCCTGCGTCTGTGCCCCAAGCATCCCTGT







GGTGTCTGCTCTCGGTGTACATGTGGGCTTTCCTACAGTGCTCAT







GCATATCAAAGAGGGTGAGTGATATTTATTGAGCACCTACTGTAT







TCCAGGAACTGTGTAGACAGTCTCATGTGCAAATCTGGATGTGCT







GGGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the brain comprises or consists of sequences from the gene KIAA0930. In some embodiments, the sequences from gene KIAA0930 comprise or consist of:











(SEQ ID NO: 492)



AGAGCTTGGCTGCATATTTTGAAGTGTTGAATCTGTCTAGAGAGG







GGACAGACCCACCTATGTTGATCCTGCTGTGTGCCCGTGGTCTCG







GTTTGTCTGCATGTGCCCCGAGGAACCTGTGGCTCAGAGCCCCCG







CCCATTGTCCCATCACCAGCGGCAGCGCTGGAATGGAAACCCAGA







TCTTCCCTACCAAAGCCTGTGGGCTTTTTCTCTTTTGCAGGAAAA







TATTACAAATATTTATCATAAAGAAATACCATTTGTAATAGAAGG







AGAAGTCCCTGTGAGGATCAGATGAGCACGAAGCTGCGCAGACGT







GGGGCAGGGGTGGGGCGGGTGCTGTCTCCCTGCGGTTCCCGCTTG







CAGCCTTGGCTGGCTGGAGGGCGGCTGTCCCTTTAAGTCGGGTTC







CCGCTGGGACTGCCGCAGCCAGGGGGAGTGGGTGGGGGTGAGAGA







TGTTGGTGAGAGCCCTGCCTCTCCAAAGGCTCCCCCCCAGCCAAG







CCTT






Intronic domains that promote trans-splicing primarily in liver tissue


In some embodiments of the compositions of the disclosure, there may be an intronic domain that promotes trans-splicing primarily in liver tissue.


In some embodiments of the compositions of the disclosure, the intronic domain is derived or isolated from human genes that feature exons that are primarily included in liver tissue during the process of RNA splicing.


In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MASP2. In some embodiments, the sequences from gene MASP2 comprise or consist of:











(SEQ ID NO: 493)



GTGAGTTAAAACAAAGTACACCACGATCAATGACGATTGCTTTGA







AATGTAAACATATTTCTATCTGCAGATATGTGAAAGGAAAGTGAG







AAAATCGTGTGTCAGCATGATTTCTTTTGTCTATCTTTAAATGAT







AAAAAGCCAAGAAATAAGTTTTCAGAGGACAAAAAGGATGGCTAT







GGAAAATGTGATTCTGAATCCCGCCCTTTTTTCTTTCTTTCTTTA







TACTTAACTTTAAATTTACATAAGCGAGCCCTTTGTTTTAAAGCT







AGTTTGGAAAGCTGGTGATTAAAATGGGCAACTTGGCCAGGCGCG







GTGGCTCACACCTGTATTCCCAGCACTTTGGGAGGCAGAGGCAGG







CAGATCACTTGAGGCCAGGAGTTCAAGACCAGCCTGGCCAACGTG







GTGAAACCCCGTCTCCACTAAAAATACAAAAATTAGATGAGGTAG







TAGCCCCGTCTCCACTAAAAATACAAAAATTATTAGGTGGCGGGC







GCCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MASP2. In some embodiments, the sequences from gene MASP2 comprise or consist of:











(SEQ ID NO: 494)



TTCACAACAGCCCTGGGGCAACTATTATCGTCAACCGCCCCCCAT







CTTGCAGATGAAGAAACTGAGACTCAGAGAGGTTACACATAACCA







TGGGGAAATGGAGAGCTAGGATTTAAACACAGCTCCAGTCCCATG







CAAGTCCCAAAGCCTCCCCTGAGCTCTTTTCCTCGGAGTTACACT







CTCTCTTGGTTTTGAGCCATGGCTCTGAAGTTCCTGGCATGGAGC







AGATGTTCTATTTGTGGGGACTGCACCATCTTCCCACATAACCCA







GCTTTCTCACATACCTTTTGCTCAGTCCCAGGGAAAACAAGAAAC







CTTAATTTGCAAAAGCACCTGAGAGTTTAATCTTGCCTTTGGTTT







TAAAGAACCCTACCCCACGTATGGTGAGCTCTCTTTACATGTTAA







GTATTCTTTATAAACTAAGAACTTCAGATGGCTCGATTCAGTCTT







GGCTGCTGTAGACCACATGAAAACCAACAAGCCTGGTTTCCTCTG







CGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene AGMAT. In some embodiments, the sequences from gene AGMAT comprise or consist of:











(SEQ ID NO: 495)



GTAAGGAGATTCCACCAGTGAAGATCCTGGGGGTGGAGACACTTA







ACACCCATTTTCCTGGGACTTGAAGCGAATCTTTAGGAATCAGGA







TAGGATTTTTGTTTGTTTGTTTGAGACGGAGTCTCACTCTGTCGC







CCAGGCTGGAGTGCAGTGGTGCGATCTCGGCTCACTGCAACCTCC







GCCTCCCAGGTTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAG







CTGAGATTACAGGCACATGCCACTACGCCCGGCTAATTTTTGTAT







TTTTAGTAGAGATGGGGTTTCACCATGTTGGTCAGGCTGGTCTTG







AACTCCTGATCTCGTGATCCACTCACCTCGGCTTCCCAAAGTGCT







GGGATTACGGGTGTCAGCCACCGCGCCTGGCTGCCAAGGTAGGAT







TTTAAGAGCAGAGCACCTAGGCCTTTTCTGTTCTCTGGGCTGAGG







ATAAGTTTGAATACCCTCTTACTGAAGGGTGATTGATTTTGGCAA







GGGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene AGMAT. In some embodiments, the sequences from gene AGMAT comprise or consist of:











(SEQ ID NO: 496)



TGAGATCGCGCCACTGCACTCTAGCCTAGGTGACAAAGTGAGACT







CCATCTCAAAAGAAAAAAAAAAGAGGGGAGGAAGTCCGGACCCAA







GATGAGTTGCCACCTTCTTAGTTCTGGTCCTCGTACCCTCTAGGC







AGAATGGCTCTCAGATAGACGTGTATGACAAGACCCCAATGGAAC







AGACCCAAATCCCTTGCTTTTAGAGCGTGCCAAGGCCAGTCTTCT







GGACAGAGCAATTCAAAAGGTGCTAGCGTGAGCTGCTGTTTCCCA







CTCTTGCTTTAGCAAAGCTTCTCTCTGATTCCAACTCCATCAGTC







AGCTTTGACCCTGCTTCCTTACCACTCATTGTCCCTCCCTTCCTC







ACCTGTAAAATAGGATAGTTCCCTCTTCATGGAGATGGAGGGTCA







AGTGTCATGTGCACACAGAGCTGAGCATGGGGCTGGCAAACAGGA







AATGCCCTGTAAATGCTGCCAGACAGAAACCTCAGTCCCTGTCTC







TGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MFSD2A. In some embodiments, the sequences from gene MFSD2A comprise or consist of:











(SEQ ID NO: 497)



GCTTCCGCAATGAATTCCAGAATCTACTCCTGGCCATCATGGTGA







GTGGGACCTGAGCAGGGGCGGGCAGCCTGGGCTGAGGTGACATAG







GCTGTGGAATGGTTCTTGGAATAGGCAGAGGATGTTTCTCAGGCT







GGCCCAAGGTCATGAAAGGATGAGGGAGGCTTCTCAGGGTATTTG







GTTGGACCTTGCGAAGGTAGGACTGTGAAAGAAAGAGTTTGAGGT







TAGATGTTTGAGTAGTGAGCACAGCAAAAGCAAGGGCGAGGACAC







CAGGAACTTGGCTCAGAGGTTTGAGAGGGCAGCAGACAAGGTGGG







CCTGGACCAGCTCTGAGCTCCCCTGGGGAGCCGTCAAAGGTGCCT







GTCATCTTCGTTGCTGCCCACATGATGTCATCTGGCTGCTCTTGG







GCAGGGCTGGGAGCCACTTTGGGGTTCTGGGAAGGGCAGGAGGGG







CTCTGTGGCTCTAAAGCACCTCCCTTTAACCCCCTTTGTCCATCC







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MFSD2A. In some embodiments, the sequences from gene MFSD2A comprise or consist of:











(SEQ ID NO: 498)



TGAGTGGGGTTGAAGAGCAGAGCCTGGGTTGAGTTGGGATGTCTG







GTGGGAACCTCCCAGCTGATTCATCTTCCTGCACCCCCTTCCCTA







GTCAGCAGTGCCATTTCTCATCTTGGTGGCCCTCATGGAGAGTAA







CCTCATCATTACATATGCGGTAGCTGTGGCAGCTGGCATCAGTGT







GGCAGCTGCCTTCTTACTACCCTGGTAGGTATATACAGGCCCCCC







TCCTCGGGTATCTCCAGCCCCTAGTCCCCAGTTTTGAAGCTCCTT







AGGGAGAGTTCTATGCAGTGTTCTCCCACAGGCCATTCTGTGGGT







CCAGGTTAGGAGTGGGGGAGGTCTGTCCTGTACAGTTGTACAGGT







AGTGAGCTTTGCAAGAACACCTAGTCAGAGTGAAAAATGGGGGCT







GTAATCTGGCCTGGGCTCCACTTGCCACGCTGAGCACCTCCAGGC







AGGGTTACTTCCTCTTAGAGCAAGAGGCCTTTTCTTATTCATGTG







AAGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene EBNA1BP2. In some embodiments, the sequences from gene EBNA1BP2 comprise or consist of:











(SEQ ID NO: 499)



GAAGCGGAAGTCCCAATCAAAAGTTGAGCAGTAGCTGTGAGTTGC







TGGCGCTGGAGCGATTCGGTGGCACGTGGAGCCACGGCGTGGGAG







TAGGGGGCTGAAGGCAGGCAGCAGCGGCCAGGGCCGCCCTCTGCT







AGCCGCTTGGGTCTCGGGATACCCCGTTTCTTCCTGTAGGTGTGG







GACGTGCGTGCGGCGAGATGGACACTCCCCCGCTCTCGGATTCGG







AGTCGGAATCCGATGAATCCCTTGTCACAGACAGAGAGGTAGGTG







TCAGCCGAGCTCGTGGGGCTGGGCGTGGGAGAGGGGGCCTTACAC







CCCTGGGAGTGACCGTGATTGTGTCCTGCAGTTGCAGGATGCGTT







TTCCCGAGGGCTTCTGAAGCCAGGCCTCAATGTCGTGCTAGAGGG







GCCGAAGAAGGCCGTGAACGACGTGGTGAGCGTGGGCACAGGGTC







GGGGGTACTCGGACATGGGATGCGCTTCAGGCGCTGTTGGGCTTG







GGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene EBNA1BP2. In some embodiments, the sequences from gene EBNA1BP2 comprise or consist of:











(SEQ ID NO: 500)



AGTTTCATAATACAAGTCGCCATTTATTTCCTCTACATTTATAAT







ACTGCATATATATTCAAATTGGCCAAAATCTTTCTTTCCAATCAT







CATGCTGGGATTATGGGGTTATATGTGGCACATACCATTCCTGTA







TATGTCAGGATGGAAAAAGTTTTACCTGGAATGAATTTTTGCCAT







TTCTGATCCACATTGTACTTCACACAGTGATTTCCTGAAGGAAAT







ATAATGATCTGTTCATCGAAGTAGAAGATATTGTTGGCCACGTGG







GATCGAAGACCAAAAACATGCAGCGTCTGAGCTACCACGGCTGAC







ATGATCTCCCGCCAAACAGTTCTGCTGAAGACCAAGGCAGCGCTT







CAGAGTGGAGAGCACCGCCCCGCTCCGACCCCGCCCCCGGCCCGG







ACTCGCCCTCCCCGCCCTTTGCTCCTCCCCTTGTCTCTCCCCTTT







CTCCTCCCCTTGTCCCTCCCCTTTCCCCTCCCCTTGTCCCTCCCC







TTTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PRDX1. In some embodiments, the sequences from gene PRDX1 comprise or consist of:











(SEQ ID NO: 501)



GTGAGTGAGACTCCGTCTCAAAAAAATGTGGAATAGAACAAACTT







TAGTTTTAGGAACTAGACTTTATATTGTAGATCTAGTTGCAATTG







ACACGTCCTTGTTT







TATGGGAGGCTACATACTTTAAAGGTTTCCAGGGGAGCAAATAAT







TAATTATGATTTTGTGTATGTGTGTTTGTGGGGGGTAGTCATCAG







GAAATGTCTGAGGCCTTTTGCCTTACCGTGTGGGTCTGGCTAATG







TGTAATGTTTTGACTCAAGATTGTAAAGTCAAATTATTCATCCGG







GGAGAGGATGGTCGTGTAACTGAATCAGCCTCCCAAGATGAACTT







TCCCTCTGGCGTAAGTTTAGGAGTCCTGATATTTTATTTTCCTTT







ACAACAGAAAGCAGCAACCCTGTAATTAAAGAGAACAATGAAGGA







AGCTATCTAAGCCATGAGGGTTACTGAGGATCTGCGCTGGGGTTA







AGGCTGCGAACTTAAGAAATATTTGGATAGACAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PRDX1. In some embodiments, the sequences from gene PRDX1 comprise or consist of:











(SEQ ID NO: 502)



GCCTCCGGGTTCAAGCGATTCCCCTGCCTCAGCCTCCCAAGTAGC







TAGGACTACAGGCGCGCCACCACACTCGGCTAAGTTTTTGTATTT







TAGCAGAGACAGGGTTTCACCATGTTGGCCAGGATGGTCTCGATC







TCCTGACCTTATGATCCGCCCTCCTAGGTGGCCTAAATCTACATT







TTTACATAAGATAAGGTGAATGTTTGAAGAAAAAGGGAATGGAGG







AAGAAAACAATTATGTGGTGGACTCAGGGTAATCTAGTAGTAAGG







TACTACTAGAAAGGTAGAAAGTAGTCTAGTAGAAAGGTAATCTAG







TAGTGAACATTTATGGAATTTGTGGAATAGAGGCCAGGTGCGGTG







GCTCGCGCTTACATTCCTAGCACTTTGGGAGGCCAAGGCGGGCGG







ATCACCTGAGGTCAGGCGTTGGAGACCAGCCTAGCCAACATGATG







AAACCTCGTCTCTACTAAAAATACAAAAAATTAGCTGGGCGTGGT







GGTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene FGGY. In some embodiments, the sequences from gene FGGY comprise or consist of:











(SEQ ID NO: 503)



CTGCCCAAGACCATGGGAACCCACCTCTTGCATCAGTGTGACTTG







GATATGAGACCTGGAGTCAAAGGAGGTCATTTTGGAGCTTTAAAA







TTTGATTGCCTTGCTGGATTTTGGACTTGCCTGGGCCCTGAAACT







CCTTTGTTTTGGCCAATTTCTCCTGGAATGGCTGTATGTACCTAA







TACCTGTACCCGCATTGTATCTAGGAAGTAACTGACTTGTTTTTT







ATTTTACAGGCTCATGGGTGGAAGGGACTTGACTTGTCTCAGATG







AGACTTTGGACTGTGGACTTTTGGATTAATTCTGAAATGAGTAAA







GACTTTGGGGGACTTTTGGGAGGGCATGATTGGTTTTGAAATGTG







AGGACCTGAGATTGGGAGGGGCCAGGGGCGGAATGATAATGATTT







GGCTGTGTCTCTATTCAAATCTCAACTAACTTATATCTCCCAGAA







TTCCCACATGTTGTGGGAGGGACTTAGGGGGAGGGAATTGAATCA







TAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene FGGY. In some embodiments, the sequences from gene FGGY comprise or consist of:











(SEQ ID NO: 504)



TAAGTCAAATTAAACATCCTATTCTTCCCAGTCTCAGGTTTGTCT







TTATCAGCAGCATAAAAATGGACTAATGCAGTAAGTTAGTAACTT







TCCCCCATATTTTTGTTAGTGGAAAGATAGAATAGTTTTTTTTTG







TTTTAATGGAAAGATAGAATAAGAATCTTGGATTCTTTAAGGCCT







GAACTTAGATGCCAGCAGTGGCTGGGAAGGGCACAGGAAGGTGAT







AGTCCATGATGACGGGTTGATACAGACTTACTTAATATATGTTGA







CTATGTTTTATCCTTATTATGCCTTCTTATGCCTTCTTATGCCTC







TTTCTTTACCCTTCACCTTACAGCCAATCCAAGAGTTGGATATCT







CAAAAAAATTAGGAGGCCTGGGTGCAAATGTTTCCTTTCATTTCT







ACCTTTCCTATACCCCTATTAGGATGTGGAGGTGAACAGATGCCT







GAAAATCTTGAAAAGGTTGTCCATGGGTCCAACTCTATATTAACT







AAAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene LEPR. In some embodiments, the sequences from gene LEPR comprise or consist of:











(SEQ ID NO: 505)



TCTTTCTCCTTCCCCTTCTCTTCCCTCCCCTCCCATTATATAATC







TATGCTGTTATGTTACCTATTTTATCCACTACCCCATCTTTACTG







GAGGTGTTGTTCTAGTGGACTCTCCAGGAACTCCTCTATCCTCCT







ACTTCAATCAGGACTAGGCTTTCTCTAAAATTACTATAAGCCATC







ACTCTAAACTTTCCCTTAACTGGTTTCTGGTTAGTACTGTTTCCT







GGATTCCTTTCCTTCATCTTTCTTAACTACACCCTTAAGTAACCT







CCTAAGAAGTATGCGATAAGTCTCCATCTCTCCATGCCTGAACTG







CCTTTTATTCTGCCCTCCTCGATTGTTAGTTTGGTGGAGTATCAA







TTCTTAGAAATAGCCTTTCCAAGAGAACATTGAGATAATTCCTGC







CTTAACATATAGTATCCAGTGTTGCTTCTGAGCAGTCAGATACTA







TTGTGATTTTTACTCCTCACTGTTTACTTATAATTTCCTTTTTAA







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene LEPR. In some embodiments, the sequences from gene LEPR comprise or consist of:









(SEQ ID NO: 506)


AAAAAAAAAGAAAGAAAAAAAAATTCTCCTCATTTTTGTTGTTTTGAATTT





TCAGTGATGAATCTGGAGTGGTTCTTTTTTTATTTAATGTGGTTATTTTTT





GAAGAGACTTTCAATTCCATGTCTCCTGTCAATTCTAAGAAATTATATTAT





TTCTTTGATAATTTCCTCTCCTCTTGTTTCTTTGTTTCTCTTTCTAGAATC





TGTATGTTGGATGATGGACCCTCTGCGTAGATCTTCCACATCTCTTACCTT





TTATCTCATATTTTCGGTTTCTTTGTTTTGTGTGTGTGTGTGTGTGTGTTA





TATTCTGAGACCTTGCCATACCCTCTTCTTCCAGTGCTTCTGTTGGATTTT





TATATACTATTATTTTTGTAAATTTCCAAGTATTCTTTCTCAGAGTATTCT





GTTCTGGTTTTATGCATATTTTCTTAAATGTTGCTAGAAATACCAATTCGA





GGATTTTTGTTCTTTTATTTTAAAAATTCTTTTTTCTTCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene GBP7. In some embodiments, the sequences from gene GBP7 comprise or consist of:









(SEQ ID NO: 507)


AAAGAAAAACTATGAGGTTCTTGCTTCAATTTATCATGGGCTTGAAAGAAC





AGTTTGACAGGGCACTGAGCTGGCAAGGGTTTGAGGATATTGTCAGTGTCT





CATATTTGCTAGTGAGAGTGTGAACTGTTGGAATCATATATGAAATATTTA





AGTGCACATACCTTTGATCTAGCATTTCCACATTTAGAAATCTATCATATA





GATTGATTTACTCAAATTGAAAATGGTATATATAAAATAGTACTTATGTTG





CAGCATTGTTGTAATGCCAAAAGACTGAAAAAAACATTAAAGCCCCTCTTT





CAGGTACTGATAAGGGAAATCTTGGGAAACACTCATAAAGGAATACTCTAC





AGCCACTAAAATGAAGGGAGAAGCTCTATTTGAACTAACAATGACAAGTCT





CCAAGATGTATCATAAGTAAAAAAAAAAAATGATGCACGAAGCATGTATAA





TTACTACCACTGAATTTAGAAAATAATAAATGTATGCATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene GBP7. In some embodiments, the sequences from gene GBP7 comprise or consist of:









(SEQ ID NO: 508)


GAAAGTATCAGATTTGGCTTGATATGAAATGATACTGAATGATCTCAAATT





GCTCCATTTGTTTCTGTATCTGCCTGACTCATCCTATACAACTAGAGTAAA





ACATATTCCTTGTCCCAAACTGGAGCTTGACAACCAGTGTTTTATACAGGG





TCAGCAGTCCCCATTATTTGCTAGATGAGCATGGCCTTGCTATATCGGTGG





AAAGGGGAATCAATTAGAATTAAATCTGCATTCCCTCTCAAAGGGCATTCA





TTGTGCTGGAACAAAATTCCTCCCCACCTCCAGTGAGATGGTGCTGAGGAA





ATGTCACAAAGGTGTATATCATGGATACTCCCCTTATTTAGGTCCAGTCAT





CTGGAAATGTCAGACCAACTCATCAAAGATTTTCTAAGATGTTAGAGAAAT





GGACACATGTTTAACTAATGAAATTGTTAAATACAAACCTCGGTCTCTTAA





AAATTGGGGATTTTCAAAAGTAGGCTCTTTTACTTTTATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ENSA. In some embodiments, the sequences from gene ENSA comprise or consist of:









(SEQ ID NO: 509)


GTGGGTGAAACATCCCTGTGGAGGATCCCAGTTATTGAGTCTTAGTCACAG





ATGATAATGTGATGCTGATTTGCATTTACCTTGCTGGAGGCAAGGCAGGCG





TGGGCAGGCTGTCACAGTCATGGAACATGTGTGGGAGCTGTGAGAGTCAGA





AGGAAAATACATTATCCTCATGAACAGGCCCAGTTTCTATTCCTGGTCTGG





GAGAATAATCATTTTGAGGTTGTTGGCTTGTGGTTGATCTCCTGGTGGCTA





GGATAGCCCCATATTTATCCTTCTCTATATCTTATTCATTTCTGTTTCTCC





AGTTCCAGGCTATTGCCCTGTACCCAGTAAGGTCCTCAGTTACCCTTTATG





AATAAGTGATTTTGGTGGAACGATCCTCTACTGAGTGGAGTACAAAGGATG





GCAGGGGCTATCCCTTGACTCCAAAACTATATACTCTAGCTGGGAAATTGC





TTATGTACCTGAAAAAGTCAGAACACACTGGGGAGAAGAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ENSA. In some embodiments, the sequences from gene ENSA comprise or consist of:









(SEQ ID NO: 510)


GAAGTGAGCACCAGCCATTAATTATACTCCTGCAGCAAATGTATTTTTCTT





GTTCAGAGTGGTCCTCCTTACCAAGAGAGAGTCTACTTTCATAGCCATGTA





GCTCCTGATCAGATTTTTAGTTCCCAAAGGTACCTAAAGCTGACTGAGGGA





TTATATTATCGTTTTTTAAAAAAAAAATTTGGCCAGGCGCAGTGGCTCACG





CCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGAGCACGAGGTCAG





GAGATCGAGACCATAGTGAAACCCCGTCTCTATTAATAATACAAACACTTA





GCCGGGTGTGGTGGCGGGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAAG





CAGGAGAATGGCGTGAACCCGGGAGGCGGAGCTTGCAGTGAGCCGAGATCG





TGCCACTGCACTCCAGCCTGGGCAACATAGCGAGGCTCCATCTCAAAAAAA





AATTTTTTTATTTTCCTTACTCTCCTCTGCAATGACTGTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SLC39A1. In some embodiments, the sequences from gene SLC39A1 comprise or consist of:









(SEQ ID NO: 511)


GTGGCCCACGAAGCTACCCAAGACATCTCTGCACAGCCCTAGCCTTTTTGG





CTTCACCCACTCCGTTCGGGAGTTGGGGACCCGGCCTCTACATTCCTTAGG





GGAACTCCAGCTCCAGGTCCTATTTGGAGGCGGCTCCTAGTTTCCTAGTTT





TCTGGGGGCACCAGGTGAAAGGGTGATGAGATATCCATACAGCCTTTCTCA





TGCCTCGGTTTCCCCCGTGCAGTCTCCCTGAATAAGTCACTTGGGAAAGAA





AGTGTGAGAAGAGAGGAATGTTCCTGGTACAGGAGTAGATGAGTCACCCTG





GGTAGGGGAAGGTCACAAAGTGGCTGGGGCCAGGGAGCTGCACTCTGCTGA





CCAAGCTTCCCCATTCCTTGCCCCATCTTCCTTTGCATGATTTTTCCCAAC





CACTCTGGTCTGTCTTCCTAGGTCTGAGAGTCACTGGAGCTACCAGAAGCA





TCATGGGGCCCTGGGGAGAGCCAGAGCTCCTGGTGTGGCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SLC39A1. In some embodiments, the sequences from gene SLC39A1 comprise or consist of:









(SEQ ID NO: 512)


AGACTTGACAATGCGGGGTCTCCCTCTTTTAAAAATATCCATATAATCAAA





GCCTTTGTTGACTGGCCCGCTCCGGCCCCTCAGTCCTCCACAGATGCGCCC





CAGGGCCCCAGCCCCGAGGTCCTTTTCCTAATATCCGCTCTTGCTCTCGCT





CACTCTCCTCAGGTCGCCTGCTCCGCCCGCTCCCGCTCCCGATCTCTGATT





GCTCCTAACTGACGTCACTCCCGGTCTGTCCCCGCCCACTCGGTGCTGCCA





TTGGCAGTCGGTCGTGGGTACGCGGCTCCTGTGGGGGAGGGGGGAGCTATG





GGAGGGGGCGGGAATTCCCGACTCTAGGCCGGAAGCGCGCGGAGACCATGT





AGTGAGACCCTCGCGAGGTGAGTGCGGGCTGTCTGGCAGCGGGGTCCATGT





CGGCTGCCCAGGCCTCCCCGGGCCCGGGAACAGGAGTCCCAGCTCACGCAT





TCTTCATCTGCTTCCCGCGACGCTTGGCGGCCTCGCACCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene RAB13. In some embodiments, the sequences from gene RAB13 comprise or consist of:









(SEQ ID NO: 513)


GTAAGGCTCCTAAATTCTAGTCCCTCAACCCCTGGCTCTGACCCTTCCCCC





GTGCATGTCTCTGTATAGTAACCAAACCTCTCACCTCTCTGAGGCCTTCAG





GTAACTCTTCAACTGCTGCCAGCTCCTCATTTTCTTGGTACCATCGCCTTA





GTCTCCCCAAATCCACAAGAACTCTTCTCTATTTTTCTCTGTTCCCCTCTT





CCCCTACCCCCACCACCTAGAACTCTCTCGATCCTATCTCCATGTTTCCTG





TATCCCTGTCCGCCACATCTTTTTCTCCCGATCCGATGCTAGCCTCTTTCT





GTCTTGCTGAGCTTCAAACTCTCCCACTGTGTCCCCACCTCACCATCTACT





TCCCAGGTTCCCTGCCCCAGTCTCCCTGCACCTCAGCTGAACAAGGGTTTG





GGAAGGGTGGAACAAGGTGGCTGGGTGTGCTGGAGGGTGGCCCAAGGACTG





TGATCATGAATATGCAGCCAGCTTGGTGAGGAACGTGGAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene RAB13. In some embodiments, the sequences from gene RAB13 comprise or consist of:









(SEQ ID NO: 514)


TCGTCGTGGTTAAACCTCTACTTCCCTTCACCCCAGCCTCTCTGGCCTCAG





AATGGAAGAGTAAGGTCCTAAGAGGGGAGCTACAATTACCGAAAGGCTGTG





GAAAATAGCGAGAGAGACAGGCCGGCCTAAGCTAGGAAGGGAAAACCGAGA





GAACAGAGGCCGGGCTGGCAGCCTTGGCCTGCTCAAAAGGGTGGGCGGGCA





GGGGGTGGAGCCCGGAGGGGTGCCGCCTCTCCTCTTTCCCGGAGCCTGGGC





GGAGAGGGAGGAAAACTTCTTCCTGGCCTGGGCTCCGTGCCGCTCTGTTTG





CCAACCGTCCAGTCCCGCCTACCAGTGCCGGGCGCTCCCCACCCCTCCCCC





GGCTCCCCCGGTGTCCGCCATGGCCAAAGCCTACGACCACCTCTTCAAGTT





GCTGCTGATCGGGGACTCGGGGGTGGGCAAGACTTGTCTGATCATTCGCTT





TGCAGAGGACAACTTCAACAACACTTACATCTCCACCATC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene FMO4. In some embodiments, the sequences from gene FMO4 comprise or consist of:









(SEQ ID NO: 515)


CTCCCAACGTGCTGGGGTTATAGGCATGAGCCACTATGACCAGCCTAAAAT





GGGGTCATTTTAAGTGTTTACATCTAACCAACTAATAGCTTTAACACAAAC





ATGCACACACACACACACACACACACACGTACAATGTGGAGGGTATACATA





CAATATAATATAAATTATATTATTTATACAGCAAAAGCAGATGAAGAGAGA





CAGGGCAGGCCAACTGGCACAGGTGCAGGTGTCTGGTGAGTGTGGCGCAGG





CTGACAGCTGGGCCATAGAGGCATCTCTCCTCACAATTCTGGCAGTAGAGT





ACCCTGGTTAAGTGAACTGGGCGGGGAATTTTACCTCCAGAAAATTATTTT





CTGTGCTGCATTTCCATTCTGAGCTTTGACTATCATGTGCTCTGAAGTTAG





AGCAGTGGGAGGCTAGAAATGAGTGGGTGGAGAATTTAAATAAAGGAAAAG





CCACATGTGACTTTAGTGTTGTTTGTGATTTTTCCCACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene FMO4. In some embodiments, the sequences from gene FMO4 comprise or consist of:









(SEQ ID NO: 516)


TATGAAATGTAGCTTGCTCTAGGGCAAACTTACAGTATATTCTGTTACAAA





AAAAGGATTCAGAGTATAAAAGCCATTCCTAGAGAAGCAGTATGGCAGGTT





GGAAATAACTCTGCATTAGGAATCAGAAGGATACATTTTTAGTCCTAACTC





TGCCACCAATCAGACGTGGCCTTAGACAAGTCATCTGGGGACACCAGGTCT





TTTCAGTGGAACACACAGCCTGGCCCCCTACCAGTGGCAATCCTGTCTTCA





CTTAGTCCAGGAAATCTATTCTGACTTCACTCTCTTCCTACCTCTCAGGCT





GCTCTTTCTCTGTATTTGCTGGTATCGCCTCTTCCTCACTCCCAAAATTTA





GTGAGCTCCAAACTTAGTCCTCAGGCCTCTTCTCATTCCTATTTACACCCA





CTCCCAGGTGGCCTCATCCAGGCCTGTGGCTTTAAATATCATCATATACTC





AGGGCTCCCAAACTGTGTCTCTGACTCAAATCTTTCTCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CFHR3. In some embodiments, the sequences from gene CFHR3 comprise or consist of:









(SEQ ID NO: 517)


CAGATGACTATTAGAAAGATTATACCTTTCTACAGTTCTGTTTCTACATTG





TGGGCATAAGAGAAAAATATAAACATAGAATTAAATTAGCAAAATGTGAGT





ACATTTGGAGTGCTTGTAGTCACGGCTTGTCGAATGGGGGAAAGGAGGATA





AGTAACCATGACTCCAATGGAACATGTTAGCATAATCCTTTTTGATATGAA





GTCACTAGGGTGGACACAACATATTTTAGGAAAGAAGAGTTCAGGCTCTAG





AGAAAGACTGCCAGGGTTCACGTTTATCACCTCACCTGATGATCTCTGCTG





TGATGTTGGGGAAGTGCAACTGAACTTATTATATTTTTGAAATGCTAACGT





CAGTATGTAGCACAAGTTAATAACTATTAACTATTTGGATTATTTTATAAT





TTTATTTTATCCTAAACTACTCATTAGGATGCATTTTATTTGCTCATGAAA





GAGAAAATTATGATTGTTAATTGTTTTTTTCTGCTTTCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CFHR3. In some embodiments, the sequences from gene CFHR3 comprise or consist of:









(SEQ ID NO: 518)


AAAAAAGAAAAGAGGTTTTGCTAACCCTTTCAGAGCATTGGGAACACAGCC





AGAAGTGCATTAAATGTATATATTAACTTGGGCAATGTTGACACTTTAGGA





TGCTGAAGCCAGGTGCAGTGGCACACGCCTGTAATCCTAGCACTTTGAGAG





GCCAAGCTGGCAATCATCAGAGGTCAAAAGTTCAAGACCAGCCTGTTCAAC





ACGGTGAAACCCTGTCTCTACTAAAAATAGAAAAACTAGCTCGGCATGATG





GCGTGCACCTGTAGTCCCAGCTACTCAGGAGGCTGAGGTGGGAGAATCACT





TTAACCAGTGGGGCAGAGGTTGAACTGAGCCAAGATAGTGCCACTGCACTC





CAGCCTGGGCAATAGAGTGAGACTCTGTCTTAAAAATAAATAAATAGGATG





CTGAAAATTCCTATTTAAGGAAATAATTCTTTTTCTGATTTATTTAAGGCT





ACATTTGTATTTTCTTAATACAAATATTTTGAAAGTTTCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene C4BPB. In some embodiments, the sequences from gene C4BPB comprise or consist of:









(SEQ ID NO: 519)


TGACCTGACAGGAGGTGGAGCTCAGGCAGCAATGTGAGCAAAGCAGAGTGG





CTATAAATACAGCTGAAGCTTTGCTTGCCTGCCCGCTGCTCACTTTCTGCT





GTGTGGCCCAGTTCCTAATAGTCCGTGGACCGGTACCGGTCTGTGGCCCTG





GGGTTGGGGATCCCTGTTATAGAGCAAAGCACATAAGCACAATGCGTGAGA





ATTGGAAAAGTCTCTAAAATTCTTCTAGGCTCCAAAACTAAACAAACAACA





CTGAACAGATCCATTTTCATTGAGATTCTTCTTTTAGTTCATATTTATTAC





TGGGATAATATTAATAAGTATATTTAAGCCAGAAAATATAAATGATTTGCC





ATGTTGAAAACCCAAATCTTTACTTAAGCTAGCCTGGTTCTGAGCCCCCAT





TCAGAGAGCTAACTGCTGCTCACTGGCTTCAGAGTATATAGCTGGGTCTCA





GTCCTTATGTTGTAACTTGTGAAAAATTTTCTTTCTTCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene C4BPB. In some embodiments, the sequences from gene C4BPB comprise or consist of:









(SEQ ID NO: 520)


TTGGTTTCAGATTTACCTCTTATTGGGCTGAAATTGCCAACAGCAGTTGTA





TAATGCACCATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAT





GTGTGTGTGTGTGTGTGTGTGTGTTGTTCTCAGAATGTGAAAAGGCCAAAT





GTAGTGGTTGTCTAAGGCCAGAATTAGCAACATATTATTGGAGAATAGAGG





GCTTAATTTCCAGTCTGTAAGGAGAATGGAAAACTCTGATCTCAGCCGAGA





GAGTAACTCGTATTTTAACATGAGACCATGAATTATGAAACCAAAACTAAC





CGCAACTTATGAGGCAAAAATTAACCTTCCATTTTGGAAAAGATGCTTAGA





ATAGTTACATCCTTAAAAATGACCCACTGGGACAGAAGGAGAGCTAGGACC





CAAATTCTCCTCCCAGTCTTGCCCACATCTGGCTTACGGAAACAGGTTTCC





AAAGCTGCAGGAAAATTGCTTACTGCCTCTTGAATGAAAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene EPHX1. In some embodiments, the sequences from gene EPHX1 comprise or consist of:











(SEQ ID NO: 521)



AAGGCGGCAGCGGGGTTGGAAACCCACCCCCACACAGTCAACACC







TGCTGTCACACCAGGCTAATCAAGAACACTTTTCTTTGGTGCATT







AACCACCTGCTATTAAATAAAGGGCTGTTACTAGGTGGCAAAGTA







ACACACTGCAGTAGAGCCTATTTGTAATCTGAGATCAGTGGCTGT







AAGGCACCATCCTTGAGCCAAGCAAGTACTAATATTCTTGTTATC







TTAATTAGAATGCAAGAGATCGTGTTTTTAAAATTCTCTTTGAAT







CTGTCACTTTGCCCTCCTCTTCCTGGGTGATACACCTGGTAGTGC







TGGTGGGGGCCATCATAATGCCCCTTGTCCCAGATTCCCTTCTTT







TAGATGGGACTCGAGCACTGATCATTTCAGCCCTGTATCTCTCAG







GTCAGCGTGGTTCAGTTTGCTGTGCAGAGTCCAGGGGAGATAACC







ACGCTGTGCACACATGAGATTGGCTGACTTGGCAGGACTGTGCAA







TTGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene EPHX1. In some embodiments, the sequences from gene EPHX1 comprise or consist of:











(SEQ ID NO: 522)



TAAGGAGGGTGGTTTGTAAAGTTCACTGGGGAGGGTGATCCCTCT







TTATTGTTCTAATATTATGCTCGCAGCCTCCTTGACAACATCATG







CAAAATGTGTCTTACTAGCTTCTAGTGCATAAAATATTGGTGGAG







CTCTTCGCTGTGCTGGGCCAGTCACCAGTGCTGGGCACTGTGGGT







CAAGGCAGTGCCCAGGACGGGCACAGCCCTGCCTTCCCAAGCTAT







GGTTTATTGTGGTAGGATCTGCCTGCCCAGTGAAGTGTGTTCAGC







TGCTCCTGTTGCTGGGGGAAGCTCTGCTCTCTGCCTTCGGCTGCC







TCCCACACGCTCCCATGTGTCTCCCAGTCCCTAACCCCTAGTCCT







GCTTTCAATACCGCAGGGGCTTTTAGCTGACTTTTCTCCATCTTC







TTAAAGGGCAGGATTTCCTGGCAGAGACATAACGTCTTTCTGGAA







ATGAAAGAAGCTGATGCATGTTTTTTCTTTTCTGAATTACTTAAA







TAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene RBM34. In some embodiments, the sequences from gene RBM34 comprise or consist of:











(SEQ ID NO: 523)



ATGAAAGCCTGCAATGTACTTTGGAGGGTGGATGGGTGACTTATT







TACTGTGTCAGCAGAATAGACTGAAAAGTTAATACTGATTATCAT







TGCTGGAATCCTAGGCATTCTCTTCCTATGTCCCATCATACACAT







TAGTATGGGAGGTGGGTATGATTGCTGCAAACAGTCTTTGTTCTC







TGAGCACAGACAGATAAAGAATTTATGTCAATTTAATATTAAAAC







TTTATAGAAGTGAGTTGGATTTTACCAGCTCAGCACTCTGAACCA







GTTATAGCCACATTCTTTGGAGCAGGAAATAAGTATCATTTATAT







TTGGTTTTCCCATACATGATTTTTTTTTTTAACTGCATAGGCATA







GTTAGTTGACGTACCATAGTGTACATTTTTGGGCACTATTACATA







ATTAATGTATATTAGAATATAGGAAATGTTTATTCTATTTTTTTT







TTTGAGACAGAGTCTCGCTGTGTCTCACCCAGGCTGGAGTGCAGT







GGTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene RBM34. In some embodiments, the sequences from gene RBM34 comprise or consist of:











(SEQ ID NO: 524)



CCTACTTTCCTGAAATGTTCTTAAAAACTGGCAGTAGGCCGGGTG







CGGTGGCTCATGCCTGTAATCCCAGCACTTGAAGAGGCTGATGTG







GTTGCATCACCTGAGGTCAGGAGTTTGAGACCAGACTGGCCAACA







TGGCGAAACCCCGTCTCTACTAAAAATACGAAAATTAGCTGAGCA







TGGTGGCGGGCGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGTA







TGAGAATCACTTGAAGCCGTGGGGGCGGAGGTTGCAGTGAGCTGA







GATCACACCACTGCACTCCAGCCTGGGCGAAAGTGCAAGACTCTG







TCTCAAAAAAAAAAAAAAAAGCCTGGCAGTAGAGGATTATCAGAC







AATTTTTCTTAATTCCTTTTTGTAGCACTTATTTGATGTTCTGAA







GGTCAGCTAATGTGTATTTTTTTTTTTAATTTTAGAAGCTGAAGT







CGTTTTTTAAAGAGTATGGACAAATAGAATCTGTACGATTTCGTT







CTCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene KMO. In some embodiments, the sequences from gene KMO comprise or consist of:











(SEQ ID NO: 525)



TCATGAAATTTAAATGTAAGACCTTTTTGTCCAGTATAGATAAGT







TCTGAAATAAGGCAAAAGATAAAGTAAAAAATATATATATAAATA







TATAATGTATGTGCATACATTATATATGTGTGCATATACACACAT







GTACACATCTAGATATGTGCATGTGTGTGTACATATATACCAGGT







ATAATTATACCAGCTATGATTCAAGATTTGAGAACATAAGATGTT







CTCTCTGTCTTTTTCCTTACATATACACACACACGCACGTATTCT







GAAATCTTGAAATATACCTGGTATCAAAAACCTACCTAAGGTTGA







GTTTATAAAGACAGAACACAAAGTGTTTTCTAAGACAACTTCTAT







TTTATGTAGCAATAGATGTGCGTATCATCGTGTAGATGCTACTGG







TTTTAATTACTGTGGAAACTATTATAAAGCTGAGTCCGTTTTATA







GGTTTGCTATATGAATGCACTAATCAATCATATATTCTTTTTTCT







GCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene KMO. In some embodiments, the sequences from gene KMO comprise or consist of:











(SEQ ID NO: 526)



TAGTATAGAATTTTTTATCAACTGTAATTTTGCATTTGTAATTAA







TGTTGTTTATTACTTACATTTGTACTTAGAATGTATCTACCTAAT







TGATCTCTTCATTGTTTAATCCATTTAGTGATTATAGCTTTGAAA







CTTTGGGATTACTGAGGAAAAATCTGAAGGATTTCCAATTATTTA







CTTTGGAAAGGAGATTTAACTTACATGAATGGATAACAAGAGTTT







ATGTGTGGTGGTGCCTGTCATCTCCACTAAAGACAATTTAGGAGG







AAACAGCCCTTCCAGTAAAGCCAAAGAGATTTAGGTTATCTATCA







GGAAGTGATTCAGCCTTTAAGGTCTGTAGTACATTGGAATACGCA







TTATCAAAAGAGAGCATGGGGATGGGCGCAGCGGCTCACACCTGT







AATACCAGCACTTTGGGAGGCCAAAGCGGGAGGATTGCTTGAGCT







CAGGAGTTCAAGACTGATGTGGCCAACATAGCAAGACCTTGCCTC







TACT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene RMDN2. In some embodiments, the sequences from gene RMDN2 comprise or consist of:











(SEQ ID NO: 527)



ACTTGTCCTAGGAATATCACCAGTTGTTTTTGTTCTTGAAAAGAA







AAGGATAGAGAAAGAAGACTGAGGAAAATGATTATGTAAAGAAAT







CTGAAAACCATAAAGTATCTCATTGCTTTGTAAGCTTTATGACCA







TGGTAATCAATTTTATTTTAATGGCTAAATGCAAAGGCTATGTTT







CCACCCAAAAGATACTTAAACTGATCAAGTGATTCAAATGTGAAA







CAACCAACCTAAATTCTTAGGGCAGTTTGGTTCATAATATTTGAT







TTATTTAAATATATTCTCTTCACTTGTGGTTACTGTTATTTTAAT







ATTGCCCTGTTATTCTCTCATGTTTTTCTCTCAAATCAGGCTGAA







GAACTATGCCCTGGTTATTCTAATCCCAATTACATGTACTTAGCA







AAGGTAATGAAGACCACTGTTCTGCTTTAAGATCACTTTGAACCT







ATCGCATAAAAACGGATTATGTTTAATATTGGTTATTTCTCATTT







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene RMDN2. In some embodiments, the sequences from gene RMDN2 comprise or consist of:











(SEQ ID NO: 528)



TAAGTCCAGAAAGTGACAGTGAGTGCTGTTGTCTTGTTAGTACTA







TTCGAGCTCAAAACAGGAATAACTCGCTTAGATACATTTGTAGTT







TTCTCTATTCAATTTTATGTATTTATTTTTATTCTACCTTTATTG







AAGTATAATTGACAAAAACTATATGTATACAGCATGATGTTTTGA







TAGACATATACATTATGAAATGACTACCACAATCAAGCTAATTAA







CACATTCATCGCCTCACATAGTTACTTTTTTTATTGTTGAGAACA







TTTGAGATCCACTCTCTTAGCAAATTTCAAATATACAATACAGTA







TTATTAACTACAGTCACATTGCTGTCCTTTAAATCTCCAGAACCT







ATTCATCCTGCATAACTGAAACTGTGTACCCTAGACCAATATCTC







CCCATTTCTCCCACTCTTCCAGCCCCTGACAGCCACCCTTCTACT







CTCTCCTTCTATGATGGAGTTTTCTTTCTTTTTTTTTTTATTATA







CTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SPTBN1. In some embodiments, the sequences from gene SPTBN1 comprise or consist of:











(SEQ ID NO: 529)



GCTCGCCGGGCCGCCGCCGCGTGGTTGGCGCCAGCGCACTGGGGC







AGTCGCGCGCGCCCAGGTGCGGGCCGCGTTACCTCAGCAGACGCT







AGAGAGTGAATGAGCCGCGCGGGCCCGGGACCCTTGGGGCTCTTC







ACTCTCCAGGCCGTCCCGTGGGCGCGCTAGCCTTTTCAAGTGATA







GTAATCGGAGACTTTTCCGTTACATGAGGCTCAACAAAAGATTGT







AATTCCAGCAGCTCTGTTTGGGAAGGCACTACCGCGGCGAGTCCA







GGGCCCGGCCGGGGGTCGGCGGCTGCCGGGCGGCTGGGGCGACCG







CGGACCGTGCGGGACCGGTAGGGGGTCGCGGGCCGGCTAGGCTCC







CCCGCGCCCCTCCTCGTGGTATAGCCTGCATTTCTAATACAATGC







TGTTATGCTAATCAGGTGACATCACCGCCCAGCACACGGCGAGTG







GCTCCTGATAAAATTACAAACCGGCGGCCGCCGCGGGCAGCTGGG







AGGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SPTBN1. In some embodiments, the sequences from gene SPTBN1 comprise or consist of:











(SEQ ID NO: 530)



TAAGCCCCCTCCCAAAGGCCGGGCCTGTCCTGGGTGCCAACGGGG







GTTCTTGGAGGCTTTATTTGTGACCCTAATGAAGACGGGCGGCTT







CACAGCTCGGCCCCAGACCCTGTGGTTGGCTGCGGGCACCCCATT







CACGACTTAGGGAAGGCAGTTGCTCATACTCCACTCCAGAAGAGC







TACCATTTGGAGTCCCTGTCTCTCCAGCACCTTTTTGGCTTTCTA







GGTTGTCAGATAACTGCTGCACACACATTTTCTTTACTTTTAGCT







AACTTTACCTCCTAGTAGATCAGTGCTCATAGACTTATTAGTATG







CAGGTATTTGATTCCGGGTCGCCTCTGGCTCCCAGCCCCCAGCCT







CTTACCTCTGGGAAGCTTTAAAGGTGTAAAGTGCCGTGCTCAGAC







CCAGTCCTGTAGAACAATAGCAAAGGGGAGGAGGGAGGGGTCTTT







TTGTTCCTAAGCCTTTGGTAAATTACAGTTTTTTATTCAGAAGCT







TAAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene NFU1. In some embodiments, the sequences from gene NFU1 comprise or consist of:











(SEQ ID NO: 531)



GTGGGTAGTGCATCTGATGGAGCCGGCGGGTCCGCCCTGTCGCCG







TAGTTGGGGGTGAGCCCTGAGGCTGTCTGTGCGGGAGCTCGGCGC







TTCTGTGGCCTAATCCGGGCGGACTCTCTGGAGCTTCCTCTCAGG







CTCAGAGACCCAGTTCTTGAGTCTCTGCGGTACGGAAGCTTGTAG







AGACTGGCTGTAGAGCCAGAACGGAGGAGCTGTTTTAAAATCGTG







TGCCTCTCCCGCCCCCAGGCCCTCTACCGGGTGCATTCCCTCCTC







TTCTAAACGCAGAATCAGAATGTCACACAGAAGGGAAGAAAATAC







CTTGTCTCGTTTCCTCATTTTCCCAGTAAGGCCCTGAAAGAGGAG







GTGACATGTCCAAGGTTACACAGTTTCTAAGTGATGGAATTCCGA







CGAGAACCAGGACCCCTAGATTCCCTGCACCCACCACCATTAAGC







CCACTCTGTTCCCTTGTTTTTTCCGCCTCCCTGAGCCCTACCTTT







GAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene NFU1. In some embodiments, the sequences from gene NFU1 comprise or consist of:











(SEQ ID NO: 532)



CGAGAACCTTTTAGAAGGAATGCTCCAGGGAGTCTTTGGGGGCAG







TCACTGAGCTGGGCCTGGAGGCTGAGTAGGGTCCTAGAGGAGGGA







ATGGGTCAGGTTTAGGGGCTGGAAACAGCATTCGCAAGGCAGCAC







CAGCACCAGCTAAAAACCTAGCCAACAGGGAAAACAAAGTACGGA







AACACGCATGCGTTCACAGTGCTGCGTGAATTAACCAAGCGTTGG







CGCAAACGGGAAAGGTTCCCCGGCCTCTCTTGGTCAGGGTGACGC







AGTAGCCTGCAAACCTCGGCGCGTAGGCCACCGCACTTATCCGCA







GCAGGACCGCCCGCAGCCGGTAGGGTGGGCTCTTCCCAGTGCCCG







CCCAGCTACCGGCCAGCCTGCGGCTGCGCAGATCTTTCGTGGTTC







TGTCAGGGAGACCCTTAGGCACTCCGGACTAAGATGGCGGCGACG







GCCAGGCGGGGCTGGGGAGCTGCGGCTGTTGCCGCCGGGCTGCGC







AGGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PCBP1-AS1. In some embodiments, the sequences from gene PCBP1-AS1 comprise or consist of:











(SEQ ID NO: 533)



GTTGGTTGAGGGGTTTAACAAGGCTAAGAAGGTGAGTCAGGTTTA







GACTTTGGAAGTTGAACCACAGGATTTATGCTACATACAGAGGAA







TGATTGTTGCTTCCTCTTATGTTAAGTTGGTATGTCACGTAAAAC







CTTTCAGAGTGCTTTCACGTTACTTGTTTAATCATCACAGACAAC







TTTGCCAGTAGGGTCTTATGCCCATTTTACAGAAGAGCCATTTTT







GGATTAGAGGAGTGAAGTGACTTGCCCAACGTTACACAGCCTGTT







AGAATTTGAGCTGAACCTCTTGACTCTTAGTTATGGATTACACCT







TTCCATGGCTTCTGATGCTTTTGATATGGACTTTGATGTGTGGGA







TAGCTTTACCTTAAACTCAAAAGGCTTTCTAGCCAGTTACTATTT







TTCTGTCCCCAAACAGCCAATGCATTTACCTTAAGTTCCTAACTC







TTGTTTTAGAAAATCTCTTCCCTCTTTTCCCTGATGTTTACATTT







ATGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PCBP1-AS1. In some embodiments, the sequences from gene PCBP1-AS1 comprise or consist of:











(SEQ ID NO: 534)



CTACCTACTGAAATATTTCCATAATTTCCTGTTATGTACTCCTGG







GACTTAGTGCACCTCCTCAGTGAAGCCTTCCCTTTCCCAGCTTCC







CCAGCTCTTAGCCTCCCTCCTTCCCCGAGAGTCTTAACAGTTTCT







CCCTTCGGTAGCCCACGTTTGGACGTTGGTTAAATTGTAATGTGT







CTTGGGGTTGGTAACATGTCATACAGACACCTTGGGTGATGCTGT







GGTCTGGCAAAGAGGTCATTGGACCTTGGAATCTGAGGCTCTGGG







CCCTAGTCCTAGCTCTGCCCCTGTCTAGCTCTGAGACTTCTACAA







CTCATCTGTCAGGGTTTCAGTTCTCCCTTTTATAAAGCAAGAGAT







TAAATGATCTCTAAGGGTCCTTCTACTTATGTCCAGGGACTGATG







ATATTTGATGACTTTGGTTATTTTTCTTTGGCGGGGTGGGAGGTT







TATGTAAATGTTTTCTGCACTTTTGGTAAAATCTCCACTCCCTTC







CTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene IL1R1. In some embodiments, the sequences from gene IL1R1 comprise or consist of:











(SEQ ID NO: 535)



TCCTTAAGTGAAAAATCTGTGAGGAGTTTTCTATCTTTCTAAGTC







TTTGTGGCACACTTATCACATGCATAGTACTATTTCTCAAGTTAC







CCAGGCACACACAGATGTATGGCTTTCAGGAAGCCATACATCTGT







GTAGCTCTGAGGCCCAGCCTTGTTCAACACTTCACTCAGAAGAAT







TGCTGGCCTGATGACCAACAATAATTGTCAACAACTTAAAACTTC







ATTGACGGCTCCTAAAAAAAATCAGTGTGCATGTGTGTGTGTATG







TGTGTGTGCGTGTGTGTGTGTGTGTGTGTATGAGTGTGTGTATAA







CATTCATTACTGCAAACTGGAATGGAGTGGGGCTGGAAGGCAGCC







CAGAAGCAAGGCTCAGAGCCTGGAGCTGCTGACCTCTTGGGGACA







CTGCAGCCCTGGCCTGGCCTACTTTTCTCTTCTCCCATATCTGGA







AAATGAAAGTAGCCTGACGTATCCGGGGACCCGGCCCAAGACTCC







ACCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene IL1R1. In some embodiments, the sequences from gene IL1R1 comprise or consist of:











(SEQ ID NO: 536)



TAAGGTTTTAAAGGGCCCGCCCTGGCTTCCTTGGGCCTGTAGTTT







GTGACTGTGTCGTTTTCAGAGTGGGAAGGCCCAGAGTTGGCCCCA







TGGAATTCTTTCTGCTTCTAGGGTGTTTGAAGAGATAGAAAGCCA







GGGTAAGGTTCACCGAACCTCCAAGCAAAAATAAATTTCCAGAAG







GGGCCCCTTTACTCTGAGGACATTGAATAGCAACAAAAACTACCA







CTGAAGAGTCATTTTGTCCCAGGCCCTGTGCTGGAAACTTTGAAC







ACCTGTGTTCACCCCTCCCTCATGACACTCATGAAGATATACCAT







GGCTGTCTTTATTTTCTAGGTGGGAATCTCAGGCTTGAGGGTTGG







GGTGGCTTGGGGAAGGTGTGTATCTAAGAGGTAGCTCTTATTTAT







TTTTACTTCTAGAGATTAAGACACATTCTTTTTCAAGATAGACAT







GGAACTGTCAAAAAGGATTAAAAGTTAAATAAGGATAAGGTTATG







TAAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene DBI. In some embodiments, the sequences from gene DBI comprise or consist of:











(SEQ ID NO: 537)



AGCGCGTGCACAGCCAGGCTGCGAAGGTGCAGCGGGCGGGAGGCC







CGTTGGGGGCTCAGCCGGCTGCCAGAAGCTCTCGGGCTCTTTCCT







TCCGTGCCCCTCACTTGCTCATGGGCCCATGCCTAGCCCTGATTC







GTTGGACAGAGCCTTGTGAGCGGGATTTTCCGTTTGGGGATTTCT







AAATCTGCTGCCCACCCCGCAACTGCCGGAAAGTTGCCCATGGGG







TGGACTTCGCTGTGTAGCGGGAGAGGGGTGGGAGTCGAGGGTGCT







TGATGGAGAGATGGGGGAAGGGGTTGCACGGATTGGAGGAGCGAG







GAGACTCAGTCCCCATCCCGAAGCACAGGGCAGGACGTCGCGGCG







GAGTGGGGAAGCGAGGAGTCCGTGGCCGAGAGCTTGGAGGTCAGG







GGAAGTACGGGGCCGGCTGCTCAGAGTGCGGGACGAGGAGAATCG







CGGCCCGGGGAGAGGTGACCCAGGGGCCCCTCCCTTCTCTCCAGT







GTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene DBI. In some embodiments, the sequences from gene DBI comprise or consist of:











(SEQ ID NO: 538)



TCGGTGTTGAACGCGCGGGCCCCAGGGGGAGGGAGGGGACCAACG







GGCTCCGGCGCTGACACCGCGGCACTCATGCCCTGTCCCCTTTCA







GCTGTTTCCAGCATACTGTGCCCCGTCTGTCCTCAGGCCAGGGCT







TCGCTGCAGCCCCGGCCACTCCCTAGTGCCTGGCCCGGTGGTGGC







CAGGCAGTTGGCCGCGCTGCTTCTCCCGCAGAGGGGACCCCCACT







GGGGGCGAAGGCTTGGCCTGCCCTCTTCACTGCTGTATTTCCAGA







CCTGATGCCTGCGTTTGTGAGAGCTCTGGATATATGGTTTTCGAT







TGAATGAGTGAACTGGAGGGGCTTCCCCTTCTTGTGTTGCTGAAT







CTTTCTAGCTGCCCTGTTGGGGCAGGGAGGGGCAGACACACTTCA







GGGGCTGCATTGCCCGAAGGGTGCCACCTTTCCCACCTCTCCATC







CCCGTAACTGGGCTGTCATCAGGCCACAGTAGGATTCTTACCCTC







TCCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene HNMT. In some embodiments, the sequences from gene HNMT comprise or consist of:











(SEQ ID NO: 539)



TCCCTAGAGTTGACTATTGCCTTGATTTTGGTTTCTTTTCTTAGT







TCTCCATGTACCTATTACTATTTTGATCCCCAAGCAGATATATTA







ATCTCCTCTCAATATATGCAAACATATCAAGGAATCTGTTTATTT







TACCCCAAGGGAACAACTTTTCTGAAGCCCTCTGTTCTTTTTTTC







AGTTTAGGAAGATCTGCTTTGGCCTGCTATACAACTATTAAGGAG







GGAATTTATTTCACTTTCTGAGAATCATTTTCTTTCTCCATTATT







TGATTCCCTGATTCTATATCTCATGCCTTTCTTTTCTTTGTTTCC







TCGGTCCTTTGTTTCCTGAACAATTCTTTAAGAACTTTAAGAGTG







AACGAGAGGTAAACTTTTTGAGACTTTGTATGTTTAAAAATCCCT







TGGTTCTATGTTAATCGTTTGACTGGATATAGAACTCTATACTGA







AAATGGTTTTGTATAATAGATCTAAAGGTACTTTTCCACTGTCTT







TTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene HNMT. In some embodiments, the sequences from gene HNMT comprise or consist of:











(SEQ ID NO: 540)



CCTTGGCATGTGGAATAACTTTTACATTGCTATGTGTTTTGTTTT







CCCTATTTTGAACTTTTTAGAATCTTCTTACGTCTAGTATTCTGA







AATTTTAAAATACTGAGCTGTGAAATGGTTCCTTTTTACCCATTT







TATGGGTACTCCCTGGGCTATTTCAATTGAGATTCAGATCCTTCA







GTTATGCGGAACTTTGGTAATCATTCTTCACCACTGTTTTCTTTT







TCTGGAACTTTTATTCATCAAATACTTCCTGGATTGATTACCTAA







TTTTCTTATATTTCCAGTTTGCTACAACTTTCCTCTTTCAGCCCA







TGTATTAACTTTTTACTATTATATTTTTAATTTCCAAGAATGTTT







TCTATAGCATTCTTTTCATCATTCCCAGATGTAATACCTTCTTAT







TCATTACAAAATGTTCCTCTGCTCTCTCTGCCTCTTTCCATCTCT







GTTTCTTATGTTAGACAGCTCCCCAACCACCTAAAGAGCCTTGGT







TTTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene KYNU. In some embodiments, the sequences from gene KYNU comprise or consist of:











(SEQ ID NO: 541)



GATCTGCCCACCTTGGCATCCCAAAGTGCCAGGATTACAGGCGTG







AGCCACACACCCGGCATGTTTTCTTTTTTTGGAAACAATAACAGG







ATATCAGATTGATAAATGCAATACCAATTTCTGATAATTTATCTT







GCTATATAATACTTCATGGACTTCTTGCACTTTGTGGTGTTTTAT







TAGTCAGGAATAAGGAGAAAACAACTTATAGCTGTGGAAAGAGTA







CAAATTAATTTTTAAAAACTGGAGAATAAATCAAGCAATTCGGCT







TTTGAAGACTTTCACTTGAAAAAACACTTTTTACTTTTTAATTAT







CATTTCTTAATAATAAAAATTATGCTCAAATGCTGCTCTTATGCC







AGATTTTTAAAGAACCTTAATCTGAAAAAAAAAACTATTTTATAA







TGCAAAAGGGCTCACGGTGAAATTTAGATCGGCAATATGAAATTA







CAAATTTATTATTATCGATATGACCTAACTTGTGCCTAACTTGAT







TTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene KYNU. In some embodiments, the sequences from gene KYNU comprise or consist of:











(SEQ ID NO: 542)



TATGCACGCCATTTACTTCTTCCCACCTTACTCCAAACATCACTC







TACTTAAGAGTGTTCTAATTGCATGACTTGTGAAGTACTTTAGCT







TGTGTCTGAGTAAAACTATTTCAAAAGTTAAAAAAAAAAAGAGTA







AACCTTGGGCCAGGCACGGTGGCTCACGCCTGTAATCCCAGCACT







TGGGAGGCTGGAGCAGGGGGATCACGAGGTCAGGAGTTTGAGACC







AGCCTGACAAAAATGGCGAAACCCCATCTCTACTGAAAATACAAA







AATTAGCTGGGCATGGTGGTGGGCACCTGTAATCCCAGCTATTCG







GGAGGCTGAGGCAGAAGAGTCACTTGAACTCGGGCGGTGGAGATT







GCAGTGAGCCAAGATCGTGCCCCTGCACTCCAGCCTGGGCAACAG







AGCAAGACTCCATCTCAAAAAAAAAAAAGCGAGTAAATCTTACAT







TTTTAAAAGAAAATCTGTAATCTTTTTTGCTTCAGGCATCTCTAT







TTAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene GRB14. In some embodiments, the sequences from gene GRB14 comprise or consist of:











(SEQ ID NO: 543)



GTAAGTTTGGTATTTTTAATTTTGAGTACTTGTGAATTGGATTTC







TCCTATAAGCAGAAATTATTTATTCCATAGCCACAAAGAGTTCTT







GATTTGGGCTTATCATAAAACAGAAGTGACATTGTTCTATAAATG







AGGTCATCACTGTATTCTAACAGTATCTATATTATACCTGGTACA







TAGTGTGTGTCTGGTATTTGTATATAAATGGACAAATAACGGAAT







AGAGTATCTTGTTCTTAACCAGTGATCACTAATATTACCCATTAT







GTCCTGATTCTAGCATCTCAGATATCAATTGATGGGGGAAAATGA







ACTTCTTTTTAAGTGTCAATATTTTGATTTGAACATATGATTTCG







AAGTATGGTTTATATTATGACATTATGTCAAATGTATTTTTTTAG







GTATGATGCAACATGTATTAAGTAAAAAGCTGTTTCTTAAGTGTT







TTGAACACTTATTTTTTTGTGTGTTTACATCCCTATTGTCCTTTC







TCTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene GRB14. In some embodiments, the sequences from gene GRB14 comprise or consist of:











(SEQ ID NO: 544)



AAAAACACTGAGAGAAAAACACTGAAATTGAAAAACACTTTCAAT







TTATTTGAAAGATAACTTTCAAAGAAATTTTCATAACTCAAATGT







GTTTCTTTTTTCCAGAGAAGTATATCAGAGAATTCCCTGGTAGCA







ATGGACTTCTCAGGCCAGAAAAGCAGAGTTATAGAAAATCCCACT







GAAGCCCTTTCAGTTGCGGTTGAAGAAGGACTCGCTTGGAGGGTA







CAAGTAGCTTCACATGCTTCAAAATATTTATGATTTCAGTTTGTT







TCACCTAAAAACTTGAGATTTGTTTTCTCCTTCCTTGCTCAGAAA







AAAGGATGTTTACGCCTGGGCACTCACGGTAGCCCCACTGCCTCT







TCACAGAGCTCTGCCACAAACATGGGTATGTCTGTTCATAGTCAT







TGCCACTGAGTAGACGGATAGACATGGAAAGGACATGTAATGAAA







TTGCAGTCATCTCAAACAAAGCTTTGCATTCATCCATCCTATTTC







TTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SPATS2L. In some embodiments, the sequences from gene SPATS2L comprise or consist of:











(SEQ ID NO: 545)



TCAGAAACAGTTATTGGTATTGCCAAAAAAAAAAAAAAAAAAGGA







AGCTAAGAAGTCACCCTTCTAAATAAATAAATAAATAAATAAATA







AATAAAAACCTGAATTGGAGAGCTGCCTGCATTTCACTCTACTGC







CTCTGGAAGAAGAGTGTTATCTAAAGTGCTTCCATCATGAATAAT







GATTGAGAAACTGCTTTTACTCTCCCGACTACTGTACTCCCAGGC







AGAAACCCACGGCTGAAGCCCACGATGCCCATGTGTAACTTGGTT







ATTACACACACGTTTGGAGCCCAGTGTGCAGACCCGCAGCGCAGA







GGAGGCGGCGGAACACCCCGGGTTGGTCGGGTTTCCAAGGGCTGA







CCCGAGCTCCAGCACTTTTTCCGCGCCTGATTTTTCAGGTCATTT







TCGAATGGGCTATCTTTGCTTACATTTTGAGCTTGGATCCTCTCC







AGAAAGCATTACTACCTTCTAAAAGGAAAAACATCTCGTATTTGC







AGAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SPATS2L. In some embodiments, the sequences from gene SPATS2L comprise or consist of:











(SEQ ID NO: 546)



TAGGAGAAGATGATTCTCTTGCAACACGTGCGGATTGTGACAAAA







TCTTTCATTAACAAGGGGAGTTTCGGTGAAGTGGAGGTTTGGGGA







AAGGCGAGGAAGTCGGTCTGGAGCAAGCAAGCAAAGTGCGGAAGC







TGTACTGGGATTCTTCTAGAAAGTGGGGTGGGAAAGGAGCTAGGG







AGGGCGTGTGGAGGGACGAGATCTGTGTCAGAACGTGCGTGTGAG







CGGATACAAAACCCGAGAGAGGCGTGAGCAGCGCTGTGTTTGCGA







GCGGGAGCGAGGGGCGCCGGCTGGGGTGTGTGCTCCTGGTGAGTG







ACACCGAGGGGAAGGCGGGCGGGTCGGCGGGCGGGGGCAGCCTGC







GAGGGGCGGGAGTGTCCCTGCGTGGGGCGGCCGAGCCGGAGTTGT







GTCAGTGAAGGAATCCAGTCCGGGGGCCGAGCTGGCTGCGCCCTC







CGCTGCAAGCGCCGGCAGCGCGGGGCGAGCTCCGGACGGCGCGCG







GCCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CPS1. In some embodiments, the sequences from gene CPS1 comprise or consist of:











(SEQ ID NO: 547)



AGGGCTGAGGATCGCTCCATCTTCTCAGCTGTCTTGGATGAGCTG







AAGGTGGCTCAGGCACCTTGGAAAGCTGTTAATACTTTGGTAAGG







AGAGAAACAAGTATCTGTTTCTAATGTTCTATTTTGAAGAGCTGC







AACCTGAATGTTGACTGAATGTTGACTATTGGATATGTTAACTAC







AGGGCAGATAGAATGCATACACTTAGTGAATTTAAAGTTGTTGTA







TATCCATGATTGCAGTTAATGCTGATTATGAGTTGATAGCAGTAT







TCTCATTAAGATTCAGACAATGAAAATAATTTCAAACCAGTTTTG







AACAAGATTTGATATTTAGAATCAGAAGCTAGGAATTTTATGTAT







TTCAGTGTCCAATTTACATTTAATGGTGCCTTTAGAGAATAAAGA







AGCTGGGCTACCACTCGAGCTAATGATACATTCTGTAATTTTATT







GCATATTTTAAACTACTCATACATTTTTATTGTTGTTTCTATTAA







TTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CPS1. In some embodiments, the sequences from gene CPS1 comprise or consist of:











(SEQ ID NO: 548)



TAACACTGTATATTGTTTTCCAAAACAATGATTCAAAAATTGGGA







GATACAGAAATGCATGCTGTATGCTAATAGCACTAGGGTTCTATA







TGTAGTAATTTATAAATCCATTAACAATTTCCAAACCATCACAGC







CAGTTTAAGGACCAATGTGTGGTCTTCTAGTTTAATGCTTTCCCT







TCAGGGAGTGGCCATAGGAAGACTACAGTTCTTCATCTAGAAGTG







TAGTGTAAGGCATCTCCTTACCAAACAGAGAGGGTAGCTTTATAT







TAAAGAACAGGACAATTTAAAAAAGACACACAAATTGAGGTATCT







CTCGCCAGCGTGTTTTCTGATACCCAGAAATGACAGCTTATCTTT







GACTATTGTCAGCACCTTCTGATAGTGCCTTTGTTTTAAAATTTT







CAGTTTTTGAGATCCGATTGTTTAATAGATAAATTATAAGGGTGA







GGAACAGGAACAGTGAGGATCTTTTATATTAAACTTTAAAACAAA







GTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PECR. In some embodiments, the sequences from gene PECR comprise or consist of:











(SEQ ID NO: 549)



GTAGGTAAACGATCTATATTGAAATTTATTGACAAATTATGCTTT







CTGATTCCATTTGTGATGTTGATAGAGGTATTTGGTAATAAGCAA







GTATAGCATAAAAGATTGACTTTAAAACATAGATAAAATGGAAAG







CCTAATTTTCTTTTTTTTTTTTTTTTTTTTTTGAGATGGAGTCTC







TGTCGCCCAGGCCGGAGTGCAGTGGCATGATCGTGGCTCACTATA







ACTTCCACCTCCAAGGCTCAAGTGATTCTTGTGCCTCAGCCTCCT







AAGTAGCTGGGACTACAGGCACAAGCCACCATGCCCAGCTAATTT







TTTGGATTTTAGTAGAGATGGGTTTTCACCATATTGCCCTGGGTG







GTCTCGAACTCCTGAGCTCAGGAGATCTGTTCGCCATGGCCTCTC







AAAGTGCTGGGATTACAGATGTGAGCCACCGCACCCGGCCCAGAA







GGTCTAATTATCATTATGGAAGTACTCTTTGATTTACAGCCAGAT







TGTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PECR. In some embodiments, the sequences from gene PECR comprise or consist of:











(SEQ ID NO: 550)



CCCGGTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGT







TGGCCAGGCTGGTCTTGAACTCTTGACCTCAGGTGATCCGCCCGC







CTTGGCCTCCCAAAGTGCTAGGATTATAGGTGTGAGCCACTGCAC







CTGGCCTCCTTTGTTATTTAAAATCGATGTTAAAAATAACCACAG







GCTTGAAATCCTTAAAATTACCAATGTGTTTTCCTTTGTAATCAA







AGTTCTTTAAAGTATTCCTCTTACGTAGTGATTTGGGGAGCCTTA







TCTGGGTCTATGCCATGTGAGATTTGCTTGATCATCAGCTGTGGC







TTGGATTTAATATTGAAGGACATAGTTTTTTCTTCGTTGTTTAGA







CAGAAGCTCAACAAGACAGACTCCATAGCAAGAAGTCTCTTGTGG







AATTCTGTCAGGTAGTTGGCACACTTGTGAGCTTGATGAACAAGA







GAAATATTCAACAGAAGCTGATTGTCATGTTTATGTTTTGTCTTT







CAAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SP100. In some embodiments, the sequences from gene SP100 comprise or consist of:









(SEQ ID NO: 551)


GTAGTTATTTTCAAATCTTTTCGAAAGGAAAGAAGGAAGGAAGGAAAGAAA





GACGCAGGAATATGTATAAAGGTATAGCAAACATCACACTTAATGGTGCTG





CTAAGTGCTTCTTCCTTGAAATTGGAAATAAGGCAAGGAAGTCCACTCTCA





TCACTTCTCTTCAACATTGTATTCAAACTCCTAACCAGTGAAATGAGACCA





AAAAATAGAAATAATTGTTTGGAAAGAAACAGTAAGTCAGTCTATTTGTTC





ATGATATAATTATTTTCTTTAAAATACAAAGGAAATCTATAAATAATTTAC





TAGAAATACTACATGAATTTAGCAATGCCATAAATTCATGGTTAATATGTT





TCAAACCAATTATATTTTCATATAATAGTAATAAGGAATTTGAAAAGCATT





ATCATTTAAAGTAACACCAAAACAATAAAATAACTAGGAATAAATTTATTA





TTTTTTAAATTTAATTAAATTAAATTTTTTATTTCAATAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SP100. In some embodiments, the sequences from gene SP100 comprise or consist of:









(SEQ ID NO: 552)


TAAGTAAATGGAGAGATATACAATGTTAACAGATTGGAAGCTCAATGTTGT





TGTCATCTCCCCCAAATTTATCTGTAGCTTCAATTCAAATCCAAAAAAAAG





TCCTAAAGTTTTTTCTTGAAGAAATTGACAAACTGATTTGAAAAGATAATG





TATAAAAGCAAAGAATCTAGAAAATCCAAGCCAATCTTTTCTAATTGACTT





CAAGACTTTTGTTAAGCCATGATATTTAAGGCAAAATCTTACCAGCATAAG





GGTAGACAAATAGATCAATGGTATAGCATAGAAAGTACATATATAAACCCA





TGCTAATATTGTAAATTGTTTTTTGAATATTTTCATGACCTTGGAGTAGGC





AAATATTTCTTAGATAGTACACTAAAGTCATTAATTATAAAAGAAAAAAAA





ATAAATTGGATTTCATCAAAATTACAGATTTCTTCCCATCAAAAGACATGA





TTAAGAAAATGAAAGAGAAGCTACAGACTGGGTGAAAATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene TRPM8. In some embodiments, the sequences from gene TRPM8 comprise or consist of:









(SEQ ID NO: 553)


CTTACTTTAAGGAAGGTCCCTGGAAGTTGTTCATTTCCTGCATAATAGTCC





GTTGATTCTTGCATTTGCTGCACTTACGTTCTAGTTCATTAAAAGAATCTA





GGCTGTTCCTTGGGGGACTCATCAAAAACCCACATTTTTATGTTATTTCAG





TTTTAATTCAGTTTTTAAAAATTTATGCAGAAACCACTGAGCAAATAAAAT





GCTCAGAAACATTCTGGGTTTTAACCTAGATTGAATGAGTTTGAATGAGTT





GAGCCTTGATCATTCAACCTTAGTCACGCAGCTCACACCGATAAACTGGTC





AGAATACACATCTTTAAGAGAAAAAAACATTCTCAGAGCTGCTTCTATAAA





AAAAAAAATCTCTCAACTTTGGTGGTATATTTGGATCTATTCCCAGTATAT





TCCCAGTATATTTTGAAGCAAACCATATCCTGCCTGAGAATGGAAGTGGGA





AATCTATTCTTAACACAGTGTTCTTTCTTTGTTTTTACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene TRPM8. In some embodiments, the sequences from gene TRPM8 comprise or consist of:









(SEQ ID NO: 554)


GTGTTGCTTTTGTCATTTTATTGAGAGTGACAAAATAAAATTCCTTAATTC





GGTCAGATTCACTTCTATAGGGATTTGGTTCCCAGAGTTACATGAACTGAC





TTCTCTAAGCAGATGACTAGAGCATTCTGATTAAATGCAAGCAAATGAATT





CCCCCCTCTGGTTCTGGTTCTTGTCAACATGGCCAGATCTGAATTCTGTGG





CCCACTTTTTGGTTTCCCCCAAGTCAGGCATCCATCTGTAGCCCCGGGGAC





TCCAGATTCATTGACTCAGAGCCACCCCAATGTGTCATGGGGCTTTTTTGA





GGCCTCATGTGGGCTCACAAACAGGTGCTCCTGCTCTATGTTGGGGTCAAC





ACGAATGTTGATTGTCCACAAACCACTTGACAATGGAGCTTCGGGCCCAGC





AAAGGACATGATTCCCTTGTTGTAGGCATTGTCTCCAGAGTGTTCCTGGGC





CCTCCTGCTCCAAACACACATTCATTCCAGGAAGAATCTG 






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SEPT2. In some embodiments, the sequences from gene SEPT2 comprise or consist of:









(SEQ ID NO: 555)


AGTACATTCCTCACATTTGTGAACAGTTATGTTTCTAATTATATGATGTTC





AAGTTCTGTAGTCTCATAGACCTGGATTCAAATCTTGGCCCTGCTCTGGAT





AGTACTCTGACCTTGGGCAAATTAGAGTTTCTTTGAGTACCCTCAAAGATC





ATACTGCAAACATAGTAGTACTGTAGGTACTAAACACAGAATGATGTTTGT





GAGGTACTTAGCCCAGTACCAGGCCTTATGTAAACACTTAACCGATTGAAT





GGTGTCATATGAATAAGGTCATGACAAGGTTTTTCTTTTTATTTAAAGGTG





AATCAGGTCTAGGAAAATCGACTCTCATAAACAGCCTATTCCTAACTGATC





TGTACCCAGAAAGAGTCATACCTGGAGCAGCAGGTAAAAACATTCTTATGT





TACTGTAAGTGTAATTCTACATGAAAGATGCTGAAGACTGCCTAATTAATA





TTTTATGTCTTAGCTGTGTGTTTTGTTTGTTCTTGTTCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SEPT2. In some embodiments, the sequences from gene SEPT2 comprise or consist of:









(SEQ ID NO: 556)


TAGGTGTGCTGCACCGAGGTCCTTGGATTTGGGTTGTAGACTTTCTTTGAC





ACGTATCCATGGATCCATCCTCTCTTGAGTTTGTCTGTAAAATGTAATAAG





TAGTTTCTGCCTATATTAAATATTTGATGGAAGGATTAAGTGAGAATGTTT





TACATCAAAAATGCTGTGTAACTAGAAGATTACTAGGATAGATGTTCGAAT





TCATCTACTGTCAGAAAGTTCCAGCACAGGACACCAGCATCCACAAATGAC





TGTTGAATGAAATAGGCACACATAGTGAGAGCCTAAGACTTGGGCTGCTGC





AAGAATATGTAGCCCTTTCAGAATATTTTTGAATTCAGTTTTTAAATGAAT





TCGTAAATTGCTTTCTTTTTCCCAGGAACAGAAATTATAATTTTTAACATT





TGTGTTTTTAACCTTCCCCAGTAGCACAATAATCTTAGCTTTAACCTTCCC





CTTTCTGACTTCTCTGGGATCTTTTTTTCTTAATCCCCAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene RPL15. In some embodiments, the sequences from gene RPL15 comprise or consist of:









(SEQ ID NO: 557)


CAGAGACTCCGCAGGGGGAGAGCCCGCGGTGCGTGAAACAAACCTGTTCTC





TCCTCAGACCTCAAAGACAGCGGCTCCACCGCGGTACGCGGCCACCGGCTT





TGGAGCCTGGACCCCAACTTGCCTCCTCTCGCGGAGAGACAGTCGCCGACG





CTCGCTTAGCCGCCGAGACCTCGCCGCCAACTCTCTCACCTCTCGAGACGC





CCAGGCCGCTCAGGCTCGAATCTTGCGGAGCAGGGGGCGGGACAATAGCGG





CCGCGGCGCCCCACTCGGCAGAACTCCGCCACCAGGCGCGATGCCGGAACT





ACATGTCCCATGACGCTCTGGGAGGCCGCAGCTTTCCACCGGAAAGAGGGT





GGCTGAGGTGGGGGAGGAGCCCAAAAGGCATTGTGGGAGTACAGCTCTTTC





CTTTCCGTCTGGCGGCAGCCATCAGGTAGGCTGCGTTGAGGATCTTTGCTC





TTCCATCCGCCTTTGATCGTCTTCCTCTTCAGCCATCCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene RPL15. In some embodiments, the sequences from gene RPL15 comprise or consist of:









(SEQ ID NO: 558)


TCAGGATGCGGGACAGCAAAGGGGTGGAGAGGCGGCCCCCGGGGCGGGTTG





AGCGAAGATGTGATGGCGGCGCGAATTCGAGCTGGGCCCGGATCCTGGAGG





AGGCGTGGGGCTGACGAATGGGGCGCCCGGGACAAGGCAGCCTAAAGTGAG





GCTGCTGGTTGGGTGGGGTAGATGCATTTTCCTCGAGAGTTAATCCTCGGT





GGCCGCAGCAGTACCTTGTCCTGTGATGTCAGCGGCATCTCCTTTCAGGTC





CTCCTTGGGGACGCCGCCTGCCGCAGCCACCCGCCCCCTTGGTGCTCAGTT





CTGGTTCTGTTAATTCGCCCCACCAAAACGTGCCGAGCACCGCTCTGTGCT





GGGGTTGCGGAAGTGACTGAACGCGGCTCCGTGGGCGCAGTGGTGGGGGTT





GGGCTAGCTGTCCCCGGCAGTTGGTGCAGAGCCATTTTCATTCCCGGCGGT





TTCCTTGTTTTTGTTGGGGAACTAAGATGGACGGATACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene AZ12. In some embodiments, the sequences from gene AZ12 comprise or consist of:









(SEQ ID NO: 559)


GTAACGAGCTCTTCTCTTAGGTTTTGGAGTCAGAGTTTGATTTGAATCCTA





CTCTACCCACTCTTCATTCAGTAATCAATATTTATTGAGCAACTTCTACAC





AGTGATGAGTTGGGGACACAAATGATGGGCAAAACAGACAAAGCTTCTGCC





CTCACAGAGCTTAACATTCTTCTAGATTTAACAGGGCTAATTCAACTTTCT





AAGACCTACCTATAAAATATAGATGATACATATTTCATAGAATTGTTCAGA





TAATTAAAAGAGATAATATTAAAAGAATGTAGTGCATTATAGTGGGACATA





TTGGACACTCAAATGTTAATTAATTTGTCTTCTATTCTAAATATACAACAA





ACATAGCCAATTACTGGTATGGAAAGATAGAGTATGCATTAAGACTTCTAT





AATATAATATTAATATGTTGTTGTAATGAATTATACTCAAAGAACTTTTGA





AAATTTATAAATTGAGAAGCTATGAAACTTGCTACTAATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene AZ12. In some embodiments, the sequences from gene AZ12 comprise or consist of:









(SEQ ID NO: 560)


TATTCATGTGATCTGAAATAAGGCAAAAGAAATGTTAAAGGGATTAGTTGA





CACAGGAGGGAGATTAGACATTTTGGAAGAAAGCAGGCATAGTGAAACACG





GGTTGGAAACGTGGCTGTAGTTAAATTGCCTATGTTTACATCTCAACTCTC





CACTTACTATGTTACTTTGGAAAAAATACTTAAATTCCTCCTACCTCAGTT





TCTTCATCTATAATGTTCTTGAACAGTGACTGATATTAGTAAGTAACCAAA





TTTTATTTCAAGCAAGGGATAGTTAAAAACTCACTTTCTAGTAATAATTAC





TGATCTAAGATACATGAAATTCAATTGATAATCTTTATTTAGAGTGCTATA





TTTAGTTTTTTATTTTAAACTAAAAAATATTTAAAAGGACTTTTTAGGTTC





TCTTGCATGGTCTAAAGTCCCTCTTATTCTGTTTTTCCCAGGAGGTCTTAA





AAGGTTTTAGCCACAGTTTCTCCTTCCATCATGTTTTTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene IP6K2. In some embodiments, the sequences from gene IP6K2 comprise or consist of:









(SEQ ID NO: 561)


GTAAGAATCCATTAAAGAAATTAATTTTAAATGTGCATTACCTGGTTACAT





AAAGGACTCTAGAACAACTTAGGTTCTTTTGGGACTGTTGAAACATTGTCT





TTAAGGACAGAATGTACTGGGTGCCTTTCTCTGTACTCTGAATATGCATGT





GTATTGCGGTGGGGATGGGGGAGTTGGGGGCCCTCACTTGGTCCTTCTCTG





TTGTTTTTTTCCAGGACAAAGCCAAAGGCCCCTTGTTAGCTGGCCATCCCT





GCCCCATTTTTTCCCCTGGTCCTTTCCCCTGTGGCCACAGGGAAGTGTGGC





CTGAATACCCCACCCCGGCTCCTCTGCACCCAGAGCTGGGGGCCACCTCAG





AAGTGTCATCTCTCTCTGAGCACGCATTCCCCTGCAGCAGTCGAGGACTGA





GCAGATTGAGTGATGCTGGGGCAGAGAGGCCTGGGAGGAAAGGTGTTCAGC





CAGTCGTTTGTAAGGCGCTCGTCGGCACCTGCTGAAACGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene IP6K2. In some embodiments, the sequences from gene IP6K2 comprise or consist of:









(SEQ ID NO: 562)


AAGGCCCTGGCAGCCATCCCTGGAGCCCTCACTCCCTCCCACTCTGTGCTT





TCCCTCCCTCTCCTCCTTCTCCTCCTCCTGGCCCTCAGCCCAGCATCTCAC





ACCCTCAGTTTTCAATCCCTGGTAAGATTGCTCAATTCTGTTTTGTTGTGT





GGATTTGAGTTTGATTTTGGTAGAAGTGTAGTTGTGTTTTATGTTCAGGTG





TCTCTTGATCACAGTAACCCATAGTCCCCCACTGGGGGGACGGTGGGGGAA





GACTTTGGGAGGATTTTACCCAGAATACTTGCCGCCTGCTTTTTGTCCTCC





AGGAAACCAGAAGCCCGGGTAATTAGGTAGGCCGGGGAGCAGCGTTGAGTC





AGATGGAATGCAGGAGTGGGAGGCCTGTGCTGATTAGCCCAAGCCCTTTGC





TGTTACAGAATGTCCCCAAAATTGCTATATGAAAGCAACCCTTCGATTCAC





ATATGATAATTTTTTATTTCTTTTTATAATGTATTTTATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MST1. In some embodiments, the sequences from gene MST1 comprise or consist of:









(SEQ ID NO: 563)


GTGAGCACTAGTGACGCTTCCCCCATGACCCTGCCTCAGCCCCCACCCAAA





GGCTGGCTCCCTTAACCCCAGTGAACTTTGTCTTTCAGCTGATGACCAGCC





GCCATCAATCCTGGACCCCCCAGGTTAGGAGTTGGGCCAGTTATGGGTCAG





GCCCTTTAGCCCACGACATCCACACAGTCTGGGTTTCATCCAGCCCACCCC





ATCCTACAGACCAGGTGCAGTTTGAGAAGTGTGGCAAGAGGGTGGATCGGC





TGGATCAGCGGCGTTCCAAGCTGCGCGTGGTTGGGGGCCATCCGGGCAACT





CACCCTGGACAGTCAGCTTGCGGAATCGGTGAGGCACAACTGCCTGTCTCC





CACAGAGAGGAGCTGAGGTTGTGTCCTCTGTGGTTATCCCACTGGGGGCTG





GGAATCTATCCCTGCCCCCAGAGGTCCTAGCCAGAAGATGGCAGGTCTAGC





ATCTGTCCCAGGAGTCTGTTACCTGTCCTAATTCCCCACT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MST1. In some embodiments, the sequences from gene MST1 comprise or consist of:









(SEQ ID NO: 564)


GGAGGCTGGCGGGCTAGGGCTGAGTGGAGCGCCTGCTTAGAGACCTTCGGG





AGAACTTCTGCCGGAACCCCGACGGCTCAGAGGCGCCCTGGTGCTTCACAC





TGCGGCCCGGCATGCGCGCGGCCTTTTGCTACCAGATCCGGCGTTGTACAG





ACGACGTGCGGCCCCAGGGTGAGGCCCAAGCTTGGGGGCTACAGAGCCGGG





CTGGAAGCCTGGAACCGGAGGGCCGGGGCGAGGTCTCGGCCTGATGGCTGC





CCGCACCCGCCACAGACTGCTACCACGGCGCAGGGGAGCAGTACCGCGGCA





CGGTCAGCAAGACCCGCAAGGGTGTCCAGTGCCAGCGCTGGTCCGCTGAGA





CGCCGCACAAGCCGCAGTGAGTCCCTGGTGCTCCCGGCCCCGCCAGGGCCC





TAACCCTGGGGCGGCATGCTTTGGTGTCTGGGACCAGAGCCTGGAAATGGT





TGAGACTACCCTGCCACGATTTTGCTCCCGCTTCCGCCTA 






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ITIH3. In some embodiments, the sequences from gene ITIH3 comprise or consist of:









(SEQ ID NO: 565)


ACATCCCATTGCCACCAGCCCCATAAAGGGCTTCCCTGGACGTGCCCGATT





TCCAAAGCCTAATCTTTGTAGTCACATCTCCACCTCTATCTGCCAGTGTTG





GGGCCCCTCTGGGGCCCAGCAGCGCTAAGACAGGAGGAAGGCACGGGGCTG





GGGGAGAAGGAACCCCTAGACCCTGCTCACCACTGCCCTTCGGCTTTTCAG





CCACACCGGTGAGCCCCGCCATGTCCTACCTGAGTGAGTACATGCTGGCAG





CTGCCACTCCTCCCACTGCTTAGAGCCTGCCCCTGCCACATGTCCCTCCAC





CGTGGCTACTCACTCAGCTCTCCGGGATGGGCAGATGGACAATGTGCCCTG





AGGAGATTGCGGGGTGGGAGGGCAATGGCATTAGCGAGCGGGAAGGGGAGG





GGAGGGGCATAAGCTGAACTCCTATGGGGGTCGTCGGGCGCCTCCCAGGGC





CACTTGCTTAGCTCAGCCCTCTCTCCCTTTGTATCTGCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ITIH3. In some embodiments, the sequences from gene ITIH3 comprise or consist of:









(SEQ ID NO: 566)


TGAGTCCCTGGCTGCTCCTCGGGAAGGCTCAGGGGCCTGGGCACGTGCTC





CTGCCTGTCTCGGAGTCGAGCCCATGGAGGCCAGAAGGCTGGGGGAGGCC





CCAGGTATGATGAGAGGCCCCGGGAGTCAGCTCCTTCTCTAATGTGTCAC





CAGTGGACGGGGATCCCCACTTCATCATCCAAATTCCGGAGAAAGACGAT





GCCCTCTGCTTCAACATCGATGAAGCCCCAGGCACAGTGCTGCGCCTTAT





TCAGGATGCAGTCACAGGTGAGGCTTGTGGGCTAGGGCCGGGGCCAGGGG





GCTCTTCCTGGGAGGGCTTGGGTCCCCCGAGGTAGGCACAGGGAACAGGT





TCTCACTCTGCCCAGTAAGGCATCATCTTCACATGCAAAGAAACCCCTGG





GGGTGGGGAGTGCCCAGGGCCACAGGCTTGCTCAGCCCCACATGCCAGTG





CTATTGGGAGATGATTAGCGTGAATGGACCACCTGGGCCTAGGGTAGGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ITIH4. In some embodiments, the sequences from gene ITIH4 comprise or consist of:









(SEQ ID NO: 567)


GTGACAAAGTAGGAGGTGGGGGGAGGGGAGATTGACAGATTGGGCTCAGT





CCTGGCTGTGGAGGGTAGTGCCTTGCCCAGCTCCTCTGGCTGGTGACAGC





CCTCGGGCCTCAGTTCAGTTGTATCTGATCAGGACTTGACCACACAGAGG





CTTCCTTTTCTCCAAGAAGAGGATGGAATAGACAAGGTAGTTTGAGGGTT





TCTGCCAGGGGGAGGCATGACATCCCTGGGGGGGAGCTTGAGGGAGGTCC





ATTATTGTTCATCTCCCAGCTGTTGGGGGAGAGGCTCCCCAAGTTGGCCG





GGTGTGGGGAGAGGGGACATAGCTGCCCTCTCCGCACCCACTCCTGGGTG





GATCTCAAGCCCTGGCTGTGAAGACCCACACCCCACCTGAGAGAAGACTG





CATCTGTCGATTTCTCCAAAGCTGGAGCTGCTGGCTCCCGGATGAATTTC





AGACCTGGGGTTCTCAGCTCCAGGCAACTTGGACTCCCAGGACCTCCTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ITIH4. In some embodiments, the sequences from gene ITIH4 comprise or consist of:









(SEQ ID NO: 568)


TGAGGAAAGTGAAGCCGGGTCTCCTGTCTCCCCAAATTCATTGCACCTAC





GATATAGTCCAGGATTGGGTAGCAAGATTAAACACAAGCGCTTCAAATTG





ATGAAAACCACCCTGACGGTGTCTGTCTGTGTCTCTGTCTGTCTGTCTCT





CTCTCTCTCTTTGTTTTATCCAGAAAGTAGAAACAGGAATGTCCACTCAG





GTAAGCAGCGAACATCCTCAAAGGCTGTGCTGGGTGGTGGTCTGCTAACA





CAGGGTCCAGGGCACCAGCCCTGTGAGGTTGCTGGCCAGGGCCCAGGTCC





ACCCAGGCCTCTCCAGCCCATCCCTGCTGCCAGGAGGCAGTGTAATCTGA





CCCAGCATGCTGGTGCATTTAGTCGCCTGTGCATTCAAATATTGGCTGTG





CCTCAGGCCCTGTCCCCAGCACTAAGAATACAGTCCCCATCCAAGTGGGG





CCACAGGAAGGTGGGTGCAAGATGCAAACCCAGGTCCCACTGATTTCCA 






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene IL17RB. In some embodiments, the sequences from gene IL17RB comprise or consist of:









(SEQ ID NO: 569)


TTTGTGTTAGAAGGGGAACATTTTAAAGAACCTGAGGAAAGTACCATGGA





CTGCATTCAGAGCTTTCTTTTGTTTATCTCCTTAGCATAGCTACTCCTTA





ACCTGGGGTAGCCCAGTAAATACTCTAGGCCGTCCTAAAAACCTAGAGTT





AAACATTAGAGTGTGAAGGGGCCTCACATCGGTCCCAAGTCATCCCCTGC





TTGCAGCAGGGAAGGATGACATCCAGAGACAGATGGAGACCCACCTGTGG





TCTTCAAGTGAGGTTATGCACTGGCTTGAGGATCAGGTATACTGATGGCT





AATCCATGGTCCGTCATAGCCTACAGTGGGACAGCTTAGTTCCCCCCTAA





GCTGTGAAGCAGAACCAGTTCATCTTTTCTTTGAAAGTTTGTCACTTGTA





TATGCATTTAAGGGGAAAATGGTGTTCTGCCTAATTTGGCAAAGGAAAGC





TTGTGGTTTGCCGGCTTCAACGTGACGCTTGGTTTTTTCTCTCCCACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene IL17RB. In some embodiments, the sequences from gene IL17RB comprise or consist of:









(SEQ ID NO: 570)


TAGGTGCATTAGAGAATGGGTATTTGGGGCTTTACATTGAAAGCACAGTT





GGACTTTTAGGAAGCCTAATATAGGCAATAGGTCATCGAAGACCAGACAC





AGGGTGACCCATTGCTTTATTGGCTGATGCAGATGAAGTCTCTGTCTGCT





CGAAGCAGAACTCTGGGGCTCTGTGGGAATGAGGCCGTGTTTCAGACAGG





CTAGCCTCTTGGCCAGTCAAGATGGGACTTGCCATACAAGGTGGAAAGTG





ATGCCTGCCACTCTGCAGAGGGAGATCAAGGCCGTGGAAGGAAGGTCACT





TAGAGTTGGGACCGAGAAGCAAATGTTTCATAGGAGAGCGGGTGTTTGAG





ATGGTGGCATTTGAAGAATGGGTAGGCTTCTGCCCAAAAAATGTGGGTAG





GAAGAGTGAGCTGGAGGAGAGATGGCACCAGCAAAGGTTAGGAGGTGGGA





AAGTGGGGGCTGTGTGGGCCAGCTGGCTGGAATCCAGGGCCAAAGCCTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene DNASE1L3. In some embodiments, the sequences from gene DNASE1L3 comprise or consist of:









(SEQ ID NO: 571)


GTGAGGACTGCTGTGTCAGACGAATTCCCATCTAGCAGGGGCAGGCTCTG





GTCCCTGAGTGGGATGAGTACCTCTCCAGGGAGGGGGAGGAGGAAGGGCA





TGTGCAGGCTACCACCACTTACAGCATTCTTTAAGGCCTTTCTTCAGCCC





TAAGTATCCCTTTTGCTAAATCTGCCTGGGTACCCTGCATGTGGGCCTTG





GGAATTGCCACACTTTCCCATTAATAACAGATGCCAACATTTACTGAGTG





AATAGCTAGTGCCAGGCACTGTGCTGAGACTTGACATGGACAAGTTTATT





TAATCCTCCCAATAATCCCTTGAGGTAGCTACAGTACCTTTCTCATTCCC





ACTTTGCAATTGAGAAGTGTAAGGCATAGAGCAGTCAGGATTGTTTCAGT





TGCAAGGGACAGAATCTTAACATGGCTCATGAAAAAAAGGAGCAATTGAT





TGGTTTGTGTAACTGAAATGTCCAGGAGTACTTCAGGCATAGCTGGATC 






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene DNASE1L3. In some embodiments, the sequences from gene DNASE1L3 comprise or consist of:









(SEQ ID NO: 572)


CTGGAATGTGCCTGCGAGCTGGAATGTCCAGCACAAATATCTTGGTTAAG





GTACAAGGACATAGAATGTACTATGTGCCTGTGAGCATGTCTAACAGCTA





CATAGGATAGAGCTTAACAAAGAGTTATTAGCACAAAGCAAGGAGACTTG





AAGGAAGTTAGTCTTTAAAGGAAACTATTATTTCTAACACTTATGATTTA





TTCTTTAATAAGAAGGGAAACTTTGAAGAGGAAACTTTTTTACTTTCTAC





AACAGTCTACCAGCTTTCTCTTAGCCATGAAGGGCAACTGGGAGAAATGA





GTCTTTCGTGTACTAAAAATAGGCAGCCTAATGACCCCAAGATCATTAAA





ATCTCGGCCACTGGCTGGACCCTCTGGGAACTGGGGACCCAGATAATGCA





GCCTGTGCCTTCTGGAGGGGACAGCTGGTTTCCCTCCAGCAGTGTTTATG





AGACCTTGACAGGGGAAGTGTGTGACCCCCGCCTCTTTCCTGTTTCTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MYLK. In some embodiments, the sequences from gene MYLK comprise or consist of:









(SEQ ID NO: 573)


GTGAGTGCCCCCAGGAGCTGCCCCTCCAGGCCATGCCCCCATCCCCACCT





TCCTCCTGGCCCCGGCAGCTCCAGCCCAGCAGCCCTAGATTGTTTGTGTC





CTGCAGCTGAGGCTGCCAAAGCCAGAAGGGAGAGCCTGGAAGGGGAATGA





GGGAGGGAGAGCAGGGGAAGGAGGGGGTGATTTTTTTTTTTTTTTTTTTT





GCACACACCCTTATAAGGCTACTGAAGTCATTACCGATGTAATAAACCAA





CTAAGCAAGCGAGCGCCTCTCACCAATGTTCCCATCTATAAACACAGCCT





GGCCGGCCTCCCCCATCCCCTCCCGCTCCCCTCTACCCTCCCACCCTCAG





GGATTTGCTCTCCCCTGAAGTTTCTTTCTTACCCAGCCCCTTTTCCTCCT





GCTACTTTCCTTTTTCCCTACACTGGTTGCAGGGGGAGGGAGCCTGCCAG





CTGCGAGGACCTCTGTGGTGGAGGAGTTGGTGCTGATGGTGGTGGTAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MYLK. In some embodiments, the sequences from gene MYLK comprise or consist of:









(SEQ ID NO: 574)


TGAGTGGGGGTTAAGGGTGCTTCCATTCCCGAGCAAGAGAGGGAAAGGAA





CTACTTCTGTGTTAAGGGATCATGGAGTCAGAGTCAAGGTCACTGGGCAG





GCCCACCACCAGGGCCTATCTGTCTAGACCAACCTGCTACATCACAGTTC





CCAGCAGGGTTAGCCTTTCTCCTTCCCCTTTTATTGGTTGAGTGAGTGAG





TGATTGATTGAATGATTATATTATATACTTACAGAAAAGTGTTCCTATTT





CTGGATGCCTGCCTTAGCTGTTGTCCAGACATTCTTTTTTTCTTCTTCTT





TTTAAATAAGAGCTGAGAAAATAAAGGGTGACCACACAGTGAGAGGGTCC





TGCCCTAAATCTAAGGCTGGGGTGAATGTCTCAGTCAAGCCGGCGACTTC





CATCTTCCCCGCTGGCCAGCAGCCTGTGATCAAGTCCACACTGAACAGGC





CCTTTGCCCAGAGGGCCTCTTGATCTTTATTGATCTTTGGAATTCTTCA 






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CDV3. In some embodiments, the sequences from gene CDV3 comprise or consist of:









(SEQ ID NO: 575)


TCGCCGCGCCCGGCAGCGTGAGCGCAGAGCCGGCCTCGACCCCGAGCTC





GGAGCCCCGCGGGCCGCGCCCGCCGCCGGCCCCACCCATCCGGGTCGAG





GAGGCCGAGGCCATGGCTGAGACGGAGGAGCGGAGCCTGGACAACTTCT





TTGCCAAGAGGGACAAGAAGAAGAAGAAGGAGCGGAGCAACCGGGCGGC





GAGTGCCGCGGGCGCAGCGGGCAGCGCCGGCGGAAGCAGTGGAGCCGCG





GGTGCGGCGGGCGGCGGGGCGGGCGCGGGGACCCGGCCGGGTGACGGCG





GGACCGCCAGCGCGGGGGCTGCGGGCCCAGGGGCCGCCACCAAGGCTGT





GACGAAGGTGAGGGGCCGGGAGGCCGGCACTCGGGGCCCGGGCCGCGCG





CCGGCGCCCCATGTGCCCGCCCCCGCCTGCCGGGGAAGCGTCCGGGAGG





GGCCGCGGAGGCCGGGCGGACGGGCGCGGGCCGCCACGTGACGCAGGCT





CTCCACCTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CDV3. In some embodiments, the sequences from gene CDV3 comprise or consist of:









(SEQ ID NO: 576)


TCTGGGGCCGCAGTGCCCAGCACAGAGCAGCGTTTATCGGGTGACGTCTA





GGCTGAGTAATTCCGTGTGCTGACCTCAGCCCAGTAGTTTGAGCTGAATT





TTCGCTTTCAGCAGCCGCATTTGTAGCAGCTCTTTTGTGATGACTTAGTC





TCTAAGCCCGTGAAAGAAAAAGAAAACTCTCGGTGGCAAGGTTGAAGTAG





AAATGTAGTTGTGAAATTCCTATTGACTTCTTCCTTCGGCTTTTCTTAGT





TGCTTAAAGTAACAGCTCCCATGCAGGAACCCAGCGGAAGTATAGTGTTA





GCCTACGAGCATGTTTGTCCTCCATTTAGCCTAGTTTGCCCCAGAACGAA





GCAAGGGCTTTGACCAGAAGACAAGTACATACTTAGTTTAGGTTCCAGCC





ACTTGATCCACTTTTCGTAGTGTGTTATTTGCTTAGTTATATGTCTACAA





ATAGCTTTAATTGATCAAATGGCGTTTGTTTTACAGGACGAAGATGAAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PLSCR4. In some embodiments, the sequences from gene PLSCR4 comprise or consist of:









(SEQ ID NO: 577)


GTATGTATAATTTTTGTAGTGCTCAGCAGTATTTTAATCAGATGGAAATT





TGCATTATCCAATATCTAGTTCCTTCTTTATATTGATCATGTTTTAGTGG





CTCAAAGAAAAAAAAACTGCTCTATTACCCTATCTTCAAGAAAGTAATAA





AGTGAATTATTTTGTATAATTTTATCCTTCTATACATCATATAATCATAA





GCTCTTAGATTTGAAAAGGGCTTACATTTTATCTGGTCAATAGCCCTACA





CTTTACCCATTGTTCCTACTCCACATTTACCTTTCAAAGATGAGTAAAAA





AAGGAGCCAAGAGACTAAATGAGTTAGCCAGGGACACCACAGTCAGCTAA





TCACCTCCAGATCAGGAAATAGCATTTAAACCACAGTCAGCTAATCAGTG





GCAGAGCTGGAAATAGAATCCAAATCTGCCAAATCGTAGTGCAGTGCCCT





CGCTTTGGAAGTAAGAAGAAAAGATCTCAGTCAAAATGATGCCTGTTCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PLSCR4. In some embodiments, the sequences from gene PLSCR4 comprise or consist of:









(SEQ ID NO: 578)


TAAAATAATATAAAATGGGGACACTCCATGTGAGGTTCACCCTGGCTTGT





TTGTTCTCCTTAAGCCACTGAGAGACTTCCCTTTTCTATGGCTGGTCCGT





GTTAATCGTTCTGAACTTAACTAAACAACTACAGGCTATGGATGACATGT





AGAGAGACCAGTGAGTAGGGACTTATCTAGGAACAATCTATAAGCACAGC





TTCTTCTGATGTGATTACTACAGCAAGTAGATGTTTTGTTTTTCTGATAT





AGCAGACAACTTTTTTAAAGTGGTACTGAAAGAAAGTGTTTTTCTAATAT





GGACAACTCTGCTAATATCCATCCGATCAGATGCAGATAAGCTAATTTCA





GTTGGTAAAATTTTTTTGATTCTATTGACTTTCAAAAATATATTTTGGTA





TCAATTGATGCATTTTAATATCTATTTCATCAGTTTATTTTACAGTGGTA





TTTTAGTTCTGTTATTTCTGTCTTCTGATTGCTTTGTTTTATCCCAACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene BCHE. In some embodiments, the sequences from gene BCHE comprise or consist of:









(SEQ ID NO: 579)


GTGTGTCTGTTTTTAGTGTTTGGTTATTTAATAGATGATGGGTTATGTAA





AAAAGGGATGAAGTATACTCTCCAAGGCACGGTGATGGAGTTACTATATG





TATCTCTGACTTTAAAATAACTTGGTTTAATGAACACATTATTTTTAAGA





CTTTCTTCTATCAAGTTTAGATATGTTTTGAACTCTTACTATATGACCAG





TACTTTAGTAGTTAAATAAAGAAAACTAAAATATAGATTTCACCCTGTGG





AAATATAAAATAGAAAAAGGAAACTAAAATAGAGCCCTACTTGTTATTCA





GATAATCTTTGTGGAATTAATTAATGGACAAAGAAATAAATATATCAAAT





AATCATAATGTATGGAACTAAAAAATAAGATGCATAAATTGGTAAAAACA





GATTTATTGATAAATAATAGAACCTTGATTTAAATGGAGAAAAAAACTCC





GAAGTTTACATATTTAAAAACACAATAAAAAATTTCTAAATATTCTGAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene BCHE. In some embodiments, the sequences from gene BCHE comprise or consist of:









(SEQ ID NO: 580)


TTTCCTGGAGTAATGAGATTATTACATAATAAGATTTGTAATTAAACTTG





GTCTTAAACTCAAGCAGTATTAATAAGAGTATATAGAATGAAATAGATGC





AAAAGATGAGTAAGTGATATGATTCCTTATTGTGAAATTTTTGGGCCTAG





GATAAGGGAAGATAATGAAACAAAAGTAACACCACCCTTTGAGCACGTTT





AAGTGTGAATGAAGATATATTTTATTGAAATAAAAAATTTCTTCTTCCAA





TATGTTAAGCAGAAATGCATATAAAAAATTCAACTTTAAATTTCAATTCT





TACAATTTTAAATAACATAGTGGTTCTCAGTGCTCAAACCACTAAGCCCA





GTTCACATACGTTTCATATCATCAATATATATCCACATTTTTTCTGTCTT





AAATTCTTTAAAGCTCTTGTGAACAGTGTTAGAAAACAATATTCTTTTTA





ATCTAACAATGATTTCAATTTTACTATAATGTCTCTATTTTATTATTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MASP1. In some embodiments, the sequences from gene MASP1 comprise or consist of:









(SEQ ID NO: 581)


GTGAAGTTCACTTTATAACCTACAGACAGTGACACCTCAGCTCTGCACTGG





GCCTTCCTGCTCCGTATTGTTCCCTTAGGGATTTTGAGGCTTGGGTGGGGG





TTGGTGAGTGGGAAACTAGGAGCCCAGACCCACCTCCAAAGTTCAATGGGA





CTTTGAACCCCAGAAAGCTTGATTTGTTTGTTCTACATGTCATGGACTCTA





GCCTCTAAACACGGTTAAGTTTTTCTGGTAGCTTATTAAAGGCTTAGCTTA





TGAAAATTCTCCCAGAAAACAGCAAGTTTAGACACCCCCATGGTGACAATC





CAGCTGTGCACCATGAGAACTGTACACCAAATTGGCCAGAATGAGCCAGGC





TCTTGAGAACCACAGTCCTTGCAGTAATGAACTTGGCAGTTACACTTAGAA





GTTCATATTCCTATCAGTTCAAGGAAAAAAAAAAAAAACACTTAACTAAAA





GTAAGGAGCCATAGTTTCAAGTCTTGGCTCCCACTAACTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MASP1. In some embodiments, the sequences from gene MASP1 comprise or consist of:









(SEQ ID NO: 582)


AAGGGGTTTTAGAGAATACCAAGTCCAGCCTCCTCATTTTATGGATAGGAA





AACAGAGGCTTAGAAGGGGAGCAGAGAGGAGGGCTGGCTAAAGGCACCATA





GCACATAGTGGACTAGGCTGGGCTTTTTATTTATTCGCTGTTCTTGATTGA





GCACCTACTATGTGACAGCATTGCTATAGGCACTGTGATATAGCAGTGACC





AAGAATGAGATTCTGCTCTCATGGACCCTAGGTTTCAGACTAGTTGAGGGT





GGGACAGAATAGAAAACTAAACAAGTAAGCCAGTAACACATCATAGAAACC





AGTGCCCCTAGCTCTGCTGCAGGTAATCTGTAGCCCAGCTGCCTTTCACGT





CTTCCTCCCCACCACACCACCTTTCTCTGAGAAGAACAATCCAGTCTTTCC





TCCACAGAAGAGGATGATGGAGCTGCAAGCTGCACCCCTCCAAACACCCCA





CAGATAGCTTCTCTGTTCTCACAGTATCTTCTCTCCTCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene IL1RAP. In some embodiments, the sequences from gene IL1RAP comprise or consist of:









(SEQ ID NO: 583)


AACCCAAAAGGACCTGGCCCCAGAAGCTTCTCTCTAAACTGATACAGCATC





CTGGCTCAGAAGTTTAAGCAAAAGAGACATTTTGTTTTGAAGAGAGATCTA





TAAAAAGAAGCCAGTATTGGGATTTGGTCTAGACAATGGGATAGTGTGTAG





CGTAGATGGTATAGTATAGTGTTAGAATCTTACCATTAATACTTTGTAATC





ACTGCATTATAGGTGGCTCAGCTTATCAACCTTCCTCGCCCTCACCTGTAG





TGGGGGTCTGTGGTTGCTTACTTGTCTGATTCCTACACAGTCCATAGCTCT





TGAGTCACTCTCAAATGCTTTGATTTTCTTCTCTCTATTTCTAGTATAAGT





CATAGTAGAACAGAAGATGAAACAAGAACTCAGATTTTGAGCATCAAGAAA





GTTACCTCTGAGGATCTCAAGCGCAGCTATGTCTGTCATGCTAGAAGTGCC





AAAGGCGAAGTTGCCAAAGCAGCCAAGGTGAAGCAGAAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene IL1RAP. In some embodiments, the sequences from gene IL1RAP comprise or consist of:









(SEQ ID NO: 584)


TCAGACTGTGTCTAAAACTTTCTTTGTGTCTCTCACCTAACACAGAATCCC





AGAAAATCCCTTCAAGGATGTTTTCAATGGTCTTGAGAAAAGTAATGATGA





GTTATCACACATGAAATCATGAAATCAGGTCATTCAGAATCCTCCTGGCTC





CTAGTCTATAGAGTGTGTAATATTTCTTCAAAAGTGAATAACATAGCACAT





AATTGTTACATTTTACAGAGACTTAATGCTTTTTTAAAGCTGATGAAAAAC





AGGATTCACCTCAAACTTTCATTACAACTTTAAAACTAAATAGCAAAGGCC





TGAAATGTATATGCTGCGTATGAAGTACTAGTGAATCAGTTCAGGGTAATT





CATTGCTTCTGCTCCTCAGAAGCCAGTGTGAAAAGGAAAGACTGAAGTACA





TGATGTAAGGCAGTTTCCCAAATTGAGTTCTCGGGAACACTACTAAGAATC





AGTATTGTCCTGCAATGCATTATTGTACTCTAGAATCAGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PI4K2B. In some embodiments, the sequences from gene PI4K2B comprise or consist of:









(SEQ ID NO: 585)


ATTTAGAATTCAAAGAAAGAGTCTTAACAACTAAAAAAAAAAAATGAAAAA





AAATTATTTATTGTATTTTATGTCATAAAAAGAATCTGGAAAAGTTCAGAT





AAAATTGCACTGTTACTGAATAAGCAGGATAGGTTTAAAATTTGGCCTCCA





TAATTAAATTCACCTTAATGAATATTTTTAGAAAAACGGCATTCTTTTTCA





GAGTGTCACCTTGAAGACATGTGTTTATTCTTTTTTTAAATTTTCCCAAAA





TAGCTGCTATGCTCCTTGAAAGTCTAGGGGTAAGCATTTTTTATTTGCTAT





GTATTCTTTATGTGATTATTTAAAATTAGTGTATAAAAATGGTTTTCTTGA





TAAATGTGGCTTATCTAAATTAGTGTTAAGTTTTCTTATTGTGTTTACATG





ACATATTTTTTGACAGTACTGCTGAGAAATATAAATATTAATCCCCTTGTT





CTTGTACTTTCTTTTCTAACTATAGTTCTTAAAATTATAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PI4K2B. In some embodiments, the sequences from gene PI4K2B comprise or consist of:









(SEQ ID NO: 586)


TATTGAAGTTCTCTCATTTGTTCACGTAAATCTTGTTCATTTTCAAACTAT





ATTCTTGTTATCCAAATCAAAGCGGTAAACTTTTGTTGCAGAAGTCTTGAA





GCCACTTTCTCTGTCTGTCTTGGCAAACAGATGTAGTATTGACTCTCCTGG





GCAGAATTCATCCTGTGTGGGATAGATTCTGACTCATAGCAGGCTCCTAGA





TCAGTTCCTGTCCCCTAGGGAGGAATTTCCTAGGGGCAGGGCAAAGTCAGA





TATTGGAAATGTTATCTGCATAATGTGAGTAATTAAGGCTTCATTGTATAA





ATTATGTTAGGTGCTTATTTTGACATTATGTAACTCTTAGTGTAGTCTATA





AATACACTAAACTCTTAATTTTTCCTGTGCTATAAAGGAGCAATGACGTAG





ACCAGGGATGTCCAGTCTTTTGGCTTCCCTGGGCCACACTGGAAGAAGAAT





TGTCTTGGGCCACACATAAAAACAGTAACACTAATGACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SULT1B1. In some embodiments, the sequences from gene SULT1B1 comprise or consist of:









(SEQ ID NO: 587)


GTATACTCCAGACTCCATGTCAGTTTTTTAGTATGGCCCTATCCCTTTACC





CACTCACATAGTTTATTTTTTAAAATATTTTTTGTTTCTTTAACCATATAC





TTGCAACAATCTATACTATACCCACTCACGTTGTACGTAAATCTTCAAAAG





CCATTAAGTTTTAGTCTGAGTTTATATACTCAAGTTTTCCAAACTTGAGCA





GCAACAGAATCCAAACCTCAGCACAATATTACTACTTTTTTTTCCAACTCT





CCCTAGATCTTCAGGCCTTCTGTAAAAACTGAACTTAAGGACGTTAGTCCT





TATTTGAAGATTTGGTATAATTCTTTGTTGTAACATTTGTTCCTGCTTATC





TGCTTTTGGGGAAATTTTATACTTCAAATTCTATTTATTTAATAGCTACAG





TGCTATGTAGATTATGTGTTTTTTTCTTATCTGACTTTTGACAATTTGTGA





TGTGTGAGAAACTTTTCTAAGTTGTTGACTTTATGAATGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SULT1B1. In some embodiments, the sequences from gene SULT1B1 comprise or consist of:









(SEQ ID NO: 588)


TAATGAAAATATAGATAAAACAGCTGATATTATATACAAAGTTAACATCAT





CTATGCTACAGTACCTAATACAACACGATAGCCAGTTTTAGACTTACTAGT





TATTGATACCTCTTTGGCATGAAGAAAACATTTAAATAGTCATTTATAGCA





ATGCACAGGTATAATAAAACTCCAAATTATTAAAGAGGGTATTATTATCTC





TCCTTTTGGAGATGGAGAAAGTGAAGTACAGAGATTCTGAGATACTTTCAA





AGTGTCTCACAATGCTTAAGTGATAGAGTTAGCATTTGAACTCAGGGTGGC





TAACCTCAGAATGTGTATCCTTGAATGTTACACAATACTGACTCTAGTAAT





GTGGTGAAATGCCTGTGATTGTTTCAGAATTTATCTGCATTACATAATGCT





CAAAAATGTCTTGAAAAACTGGTTGGCAAAATAAGGAAACGTAGCCTTTCT





GGAGACTCTAATATTTCCCTATACTTAACTAAATTTCTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CXCL2. In some embodiments, the sequences from gene CXCL2 comprise or consist of:









(SEQ ID NO: 589)


GTAAGTCTCGCCCATCGCTGCCGCTGCCACTGCTGGGGTCCCCGACTCTCC





CGCTGCCCCAAACCCTGTCCTCAGCCCGACCTCCTGTCTCACGAGATTCCC





TTCTCTCTGCAGAGCCACACTCAAGAATGGGCAGAAAGCTTGTCTCAACCC





CGCATCGCCCATGGTTAAGAAAATCATCGAAAAGATGCTGAAAAAGTAAGT





TATAATTTCCATGTACACAGGCGACTGGAGCTGTTGGTCAGAAATACTGGC





GTCTGCCCCCTAAAAAGTAAATCAGGAAAACCCAGGGTTAGCTGCAGGACT





GAAAAAATTATTATTTTCACAAAGTTGCCATTAAGGTTATTAATCTGTTCT





GGTGCCAGAGGATATTCCCAGTGCCCAGGGTCACCACCCAGGTTCTTCCCT





CCTGCCAGTGAATGTAGGTAAAACTGCTTTCATGTAAGGTCTCATTGGAGT





TTGCCAGTTGTGCTGAAGTGTTTTCCACCTGTTAAAAAAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CXCL2. In some embodiments, the sequences from gene CXCL2 comprise or consist of:









(SEQ ID NO: 590)


ATTCGGGGCAGAAAGAGAACATCCCACAGTTGGCGGGAGTTACGCAAGACA





GTCAGACCCGGACGTCACTCGTGAGTGCCCCGACCCCCCTCCACCCCAGAG





GCGGGGCCATCGCCTTCCTTCCGAACTCGGGATCGATCTGGAGCTCCGGGA





ATTTCCCTGGCCCGGGACTCCGGGCTTTCCAGCCCCAACCATGCATAAAAG





GGGTTCGCCGTTCTCGGAGAGCCACAGAGCCCGGGCCACAGGCAGCTCCTT





GCCAGCTCTCCTCCTCGCACAGCCGCTCGAACCGCCTGCTGAGCCCCATGG





CCCGCGCCACGCTCTCCGCCGCCCCCAGCAATCCCCGGCTCCTGCGGGTGG





CGCTGCTGCTCCTGCTCCTGGTGGCCGCCAGCCGGCGCGCAGCAGGTGGGT





CCCGGCGCCCTGGGGTCCCCGGGCCGGACGCGGCTGGGGTGGGCGCCCCGC





GCCGACAGCCCCGCTCAATCAGCGAGTCTCTTCTTCCCTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene HSD17B13. In some embodiments, the sequences from gene HSD17B13 comprise or consist of:









(SEQ ID NO: 591)


GTAAGTACAGCACAGAACACCCAAATACTAAAACACCAATAGAGCTTTTTT





TTTTGCTTTTTTTTTTTTTAGACAGAGTCTCACTCTGTCACCCTGGCTGGA





TTGCGGTGGTTGCAGTGGCATGATCTTGGCTCACTGCAACCTCCGCCTCCT





GGGTTCAAGCAATTCTCATGCCTCAGACCCCCAAGTAACTGGGATTATAGG





TGTGTGCTGCCACACTACACCCAGCTAATTTTTGTATTTTTTGATAGAGAC





AGGTTTCCCCATGTTGGCCAGGCTGGACTCGAACTCCTGACCTCAAGTTAT





CCTCCTGTCTCGGCCTCCCAAAGTGCTGGGATTACAGTCATGAGCCACCAT





GCCTGGCCCAATAGAGCTATTATTATGGAGCATCTTTCAGTTGTGAAAATT





GGCATGGAAACTCTCCATCCCTGGGGAGAACAGTTATTTCCTCTGTTATTT





TCCTACCCAGTCTATAAAAAGAGAGTGATTCATTTTCTCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene HSD17B13. In some embodiments, the sequences from gene HSD17B13 comprise or consist of:









(SEQ ID NO: 592)


TTTACCCAGACCCTATTCAAGATGGAGTTGCTCTGATTTAAACGCCTCTGA





CAAAATGACGACCTCAAAACAATCCAGCTTTATGGAATACCTCCACAAGAA





AGAAAGTATACTTAGCTATAGAATTTTCTCCTTGCATCCAACAGGCTTTGA





GATGTCAGATGTTTCCTTCCTGTCCCATGATTAATCCTAGCCATTCCTCTT





TCTTGTCTGGCTCCACTACTCCTTACCATCTAATGCCTCGCCACCATTTTG





ATATTTTGACTAAGTGAGCTATGAAACACACCTACTGGATATGAAAGTATA





AGTTTCTGATAACAAAACATCAACATGGGATGTGGAGGAAGTGGGTAGGGT





GGCATTAATGCAGCAAATCCTGGAATATTTTAAATCTTCATTCTAAATTTA





GTAAAAATATAGGATAATTTTCCTGCCATCATTTACTTATAAAATTAAAAT





TTTAGAAAATAAAAATAATATTTTCCTCTTTTTAATCACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ADH4. In some embodiments, the sequences from gene ADH4 comprise or consist of:









(SEQ ID NO: 593)


GTCAGTTTTTTTTTTCTTCATAGCTTTAAATTCTTTTCCTAGTAGTTCTGA





AATTCTTAGAGTGAAGGTTATTGAAAAGAAATAAAAATAATTTATTCAACC





ATTGAATCCCTAGAGCATATCCAAATACTACATAAAAACTATTAATGACTT





ATAAATAATTTATATAAAAAGGTAATTATAATTTGCAAATTGAAATTTAAT





TTGCATTTGAATGAACCAACATGGAGGAACAAAAAATAAACATTGACCTTA





TTGAGATAATTTCTTATATAGAGTGGAAAATAATCTTAAATTTTTCTTATA





AAGAAAAATTTTAAAATAGATATAAGTACAGAAAATTAAAGATGGAAGGAG





TTTAATCTATTTGAAGCAAACCAAATCTGACCTAATAATTTCATTCATTCA





GCAAATGCTGATTAAACTACTTCGTGTCAAGTGCCATGTCTTTATTTTGCA





TTTTGTCCACATTACAGGTTGGAAAAGTGTAGATTCTATC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ADH4. In some embodiments, the sequences from gene ADH4 comprise or consist of:









(SEQ ID NO: 594)


TTTTTTTTTTTTTGAGACAGAGTCTCACTCTGTGACCCAGGCTGCAGTGCA





GTGGTGTGGTCTCGGCTCACTGCAACCTCCTCCTCCTCCCGGGTTCAGGCA





GTTCTCCAGTCTCAGCTTCCCGAGTAGCTGGGACTACAGGCAGGCACAACC





ACACCCAGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTTGCCATGTTG





GCCAGGCTGGTCTCGAACTCCTGGCCTCAAGTGATCCACCCACCTTGGCCA





CCCAAAGTTCTAGGATTACAGGAATGAGTCACCTGCACCCGGACTGTGCTG





AACATTCTATTTGTAATGGAACACTGGGCCCTCAAGGCTCAATCTACAATG





ATACTATGATGTCATTAGCTTGAATCCCATTATCTTACATGCAGAGTTGGA





TTAATTTTTTTCTAAAGCATTGTACATTGCCACACTGGAGTTCATATTCTA





CTTTTCCACTGACTCAAGTATTCTTTTATGCTCCATCACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ADH6. In some embodiments, the sequences from gene ADH6 comprise or consist of:









(SEQ ID NO: 595)


TTTGAATGTCTTGTTATGGTGTAGCTCGTTGCAAAATTCACCTAATTTTAT





CACAAATGCAGGAACTTTTTTTCCTTCCTGTTAATATACAGTCAAACATAT





TACCAAGTACTGGAGACTATCTATATCACTGTATAACCATTCACTGTTGTT





GCCAGCTTCAAGACCACATTTGAATCCTATCAGGCCCAGTAAACTCTGTGA





ATAGACTCTGTGAACTGTCTCTAAGTCTCACTGTTTTGTCCACATTAAGTG





TCTTGTTTGAAGCACAAAGTAAAATCTTTCTTTCCAATCCCTGTCACTGCA





CCTCCCTAGACAGTCACTACATGGGGCAGTTTATGGACTTCAGCAATGTTT





ACCTAACTCCAGATCCACACTATAGGAGTCCAGCCTTCAGGGAGAACTAGT





TTTTTACATTTTTTGCCTGCATAATGAGAAGCTTTAAGTTTTTCATATTTT





TACCTGAGAACAAGAATAATTACATTCTAGGCTATCACCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ADH6. In some embodiments, the sequences from gene ADH6 comprise or consist of:









(SEQ ID NO: 596)


AAAATACATAATCCTGAAAGTATTTAAGTGTTCACCTACCGTTACTTTTGC





CATTAGCATTGTATTTCCAATATGGATTTTTTTTTTACATGACATATTAAT





GGAAGTCAGCTTATAAAATTGTAGCTCAAGTTTAAAAACATAAGAAGGTAG





TTCACAAATAGGCCATTGTTGTATTTGTTGTTCTACTTTGGGGGAGAAGAG





GAATTATTCATAGGATTCCTAATTCTGAATCCTATCTTTGACCCAAAAAAC





AGAAACCAGCCATAACCCAGAAACTGGCATTCCCCACACGAGTATAAGCTT





GCTTACCTACTTTTTACCCTTGGAGTTTCATGTCATTTTAAAAAATCTTCC





CATGGCTTATTTAATCTTCTCATTTTTCACCTAAAGAGAAAAAAAATCTAG





TTTACTGCTCTACAAAGTGAGAATTTGAAATCCAAAACACAAATGTTGCTC





TAAAAATGTTCCAACCTTTTTTCTCTTCTTCTTTTTTTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ADH1A. In some embodiments, the sequences from gene ADH1A comprise or consist of:









(SEQ ID NO: 597)


TACTTGTGTCATCTTTGCCATTGAGAAAAGCTATTTCCTATGACTTCCTGC





ATTTTTGGTATCTTCATAATCTTTAGTCATGGAATCCCAGTGGAGGGGACC





CTTCACTTGCCCTGAATATACACATGCTGGGCTATTGTGCTTGTAGTCTCC





TGCATCTATCTCAATTTTCACTGTCGGCATTTTCGCCTTTTTTTTTTTTTA





ATAAAATATACCGAATTCCTAGGGTAAAAGCTGTAGTAGGGTAAAGGATAT





ACTCATATTTACAAGTGGTGAAAGTCCAAGAGTTGTAAATCCAGGAAATTT





CTTAGGAACTCAAATAAAATGTCACATGTTTTCTTACAGTGAAGGAGAATG





TTTTTCTGACATTTATACTATGTCTTTATGGTCAATATACAATTGATTTTT





AAAAATAATAGCTGATTTCATGCTTCATATGACTAAGGTACAATTACTAAT





TGCAAAAACTGAACTATTCCTGGAATTATGTTCAAAAAAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ADH1A. In some embodiments, the sequences from gene ADH1A comprise or consist of:









(SEQ ID NO: 598)


ACTCTATACTAGATATGCGACCAGACCAAGTTACAAATCTTTCTAAGTCTC





AGTGTTTATAATCTCTAAAATCAAAATAATATGAAAATCTACCTCCTAGTG





TTGATGTTTAGGTTGAAGTTTAAGACAGAAAATGCATGAAGAATGTGACAA





ATAGCAAAAACTTATCACAAGTTATTATCCATTGTTGTTTTATATTCCTTA





TTATAACAGGAACGTGGGCACACAACAATGATTTTTTGAAGATGCTAACGT





AGTATTGAAGTCTAAATGACAGGCATACTCAGAATGTTGTGAGTGAGCTTA





AAGTTGAAGAAAACACAAGAGAAAACCAAAAAAGATGAGCTTTCCTTTGAC





TTCACTTGACCCGAATGCATGGGGGTGGGATTAGAGTGGCTGAGAACAGAC





AGATTTTCACTAAGTCATTTCAAATGCTCCTTGGACTCTCACATGGAAGCA





TGTCTAAATGCTAATACAATGTCTCTTCTTTCCTATTGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene NDUFC1. In some embodiments, the sequences from gene NDUFC1 comprise or consist of:









(SEQ ID NO: 599)


GTAAGTGTTAAGAGATCTTTTCAACTGTAAAATATAATATGTCACCTTGGA





TGAGTAGCTGTCTTCTTTAAGCTTCATCTGTAAATTGAGGCTTATAACCAC





GGCATGATATGTGCTCGTTTTGAAGATGAAATAAGACAATGAATGTAAACC





ATATGATACATTAATGCACCGTGCCTGGACCACAGAATTCAATCCAAATGC





TAGTTATTATTCAGCACGACACTTTTCTTTCTTCTGGCTCATGTAGGAAAA





TTTTAATAAACCTCTTGATGAATAGCCGTAGAGATGGGGTGAAAAACCCCC





AGAGGACAGAGAATAGGTAAACTCTTTTTTCTTTTAATTATTTTCTTCCTA





ATTCCACTGTATAGACAGAAGGCTGAGGCCGGGGTGCTAGAGAACCTTGCC





GTGCTGGAATTCACGTTGACGCCCCCACGGAGCTCTGCTGCAGAGCCCTCG





AGTCCCGCACTTCCGGCCGCCAGGTGGCGCTGGTTCTGTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene NDUFC1. In some embodiments, the sequences from gene NDUFC1 comprise or consist of:









(SEQ ID NO: 600)


TTTTGGTTTTAAAATTAATGACATAAAGTTTAAAGGATTAGTTTTAAAGGG





TCAAAATGTATTGTCTGTTACCTATAAATATTTTTACTGGTCTTTAGACAT





CAGATTGAACTGAATTGAATGTACAAAGATCTGGCTCAACTGTGCAAGATA





AAACCTATTCCTCAGAACGATGTCTTTAATAGGCAAAACCAAATAAATTGT





TTACTACTACCAGACACTTGTTTAAGCTTCTCAGCAACCCTATAGGTTTTA





TGTTACAGATGTAAAACTTAGGTATAGAGAGGTTAAGTAATTTGCCTAGAA





ATCATAGAGCTAAAAAGTGGTGAGCAGAATGGCTTTGAACGCTGATTCTAC





AGCTTATGGTCTAGGGTGGTCCAAGTCTGTTAGGTGGGCTAATGAGGATCA





CTAGATCTGGAGTCAGAAGACCTCAAACTGTATCCCACCTCTGCCATCTGT





GTGATCTTAAGCCTACCTAAGGCACTAATTCTACTTCACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SORBS2. In some embodiments, the sequences from gene SORBS2 comprise or consist of:









(SEQ ID NO: 601)


GTATAATATCACTAACTCACACAATGTCGCACTTGAATGGCTTCTAGCTCT





TCTGACTGTTTTTGTTCTAAACGTAAAAAGCAAAGGAAAATGAGATCTTGT





AGGAAACATTTTAAATGTGTAATTAAAAACAGCAGTCACTTGCCAAAAATA





GGTTGAGTAACTGCGAGTGTTAATAACTATTCACGTTTTTGTTATCTCATG





AACTTAAGTAGCATAATATTTCATTAAGATAACTCAGAGGTTTTGGGGTTT





TTTTTTTTAACCTAAAAACTTTTGGGGTAGGGGTACAGGGGTACAGGATGA





AAAAAGGGATTTGTAGGAAACTGAATCTTCATTAACTGGATTCTTTGTCAA





AGGTTGTGAACAGAATGATAGAACATTAGTTTTGAAAGAGACCTTTGCTTT





CCTTTAATCCAATCAGCAAATACCCTTCTACTATATATATTTAAACATACC





AAAATCTTGACCAATTAAGATTCACATGTACCCACAGATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SORBS2. In some embodiments, the sequences from gene SORBS2 comprise or consist of:









(SEQ ID NO: 602)


TTTACTGCAAGAATTATTCAAGCTCTCTTTGGGAATTATAACAGCTAGAAG





ATGGTAAAACTTAAATGATATTAACTTAAAAGTATGTTGTACCATAGCCTT





TAATCCTGACATGGAGCTAATCCTTGACTCTAAAAGCCAGATCCAGTGCTT





ATAGATTTAGTATAAATTGAATTTAAATGGTATAAACAAAGTTGAGTTCTG





TGCCTGAGTTTTCACAAAGAAAACCATAAAATGTTAAGTATTAAGTCTTCC





ATGCTTTCACTAAAACAGTGATACCTATTTCTAAGACAGATCCCTGGTCTT





CTTTTGTTTTGTTTCGTTTTGTTTGTTTTAGTTTGAGGGTGGTGTGAAATT





GGCAGGTGCATTCCATGATTACTAATTCTAACTGTTCATCTTTCTCATGGA





TGTCTGGCACATTTCTCTTAAAACGCATGATATTGCACGTGCTTTTCCCGT





TGCCTTGGCCTCTGCTATATCATTTTTTTGCTTCCAGATA 






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene KLKB1. In some embodiments, the sequences from gene KLKB1 comprise or consist of:









(SEQ ID NO: 603)


GATATGTACACAACTAAAACAAAACTAAGATATAAGATCATATTAATGTAA





TGATAATAAAAGTGGATTGTCTCCTGTTCTAATTTTAATAGGCACAAGGCA





TTTTTGTTAATCACTTCTTATTAAAGAATTTTTTATAATATTCAGGAAAAT





ACAACAAAAAAACCCTTACATTCCTAAGGCCTCAGAGTCAGAGTAATATAA





AGGAAATGTAAACACATGCTGAGTAATGCAAGCATTTGGCAATGGTGGTGA





CTGGAGCTGGGAGCACAGCTTTTATTTCTCTGAAATAGGAATTTGCCCCTT





AGAGTTAGACCAATTTTGCCTTCCTCTAAATGGCAAACAGTTTGGAGATAC





TTTAAAGGACATTTTTTCACTTAGGATGTTTAGTACTATGAATAATAAATA





GTCACAATTTCCTTAACTATGGTGACAAAATACAAGCAAATTTAGCCTCAT





GTCATTTCCTAAGGAACATCTTCTCTCTGTGAGTTCACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene KLKB1. In some embodiments, the sequences from gene KLKB1 comprise or consist of:









(SEQ ID NO: 604)


CGAGTATGCATGGAAAATCGCATCACAAAGGCGAGTATGCATGGGGAGCAC





TTGCTGCTGTACTTTCATCACTTTTATAGTCTGAGTTCTTAAAAGTTTCGT





TCATTTCCCTCAAAACACTTGAACCTGCAGTTTCAGTAGGTACTGTTCTGC





CAGGTGCAGATTAGTTAAGAGATTAGCAGACTTCTCTGCCTATCTTCTCTT





ACTTTAAAACAAATGTTACCATTGAATCAAGGAAGCAATAGCCATGAGAAA





AAAGAAGGATCTGACGCCTTTGAATGAAGATTCAAAACATGATCTTCATGT





TTTGTATTAGCTTGGAGTAAAATCCACTTGCTGGCAATATAGCCCTTAAGC





TTGTTGCCTCTTCTCTTTGTTTCAGAAACTAGAGCCCTGTTTATTCTGATC





AAGGCTCTGGCCCACTGTCTTTATCTCAGATAACCCACCCTCTTCTGCACA





CAGCATGGAGCTAAGAGAAGGGTGTCTAGTTATGTAATAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene C6. In some embodiments, the sequences from gene C6 comprise or consist of:









(SEQ ID NO: 605)


GTAAGAGATACCCTACAGACTGTGTCTGGAAATTGGGAAAACCAGTCTAGT





GTAACCAATTTGATGGAAGAGCTGAGCTTTTTGAAGCACAGACATGAAATA





AATGAATTTTCCACATAACTGATATTCCTGTGCTCTGCTGAATTCCTCCTT





CCCCATTGGGTAGCAAGTTTCTTAATTATCTCTCAGCCCAGTTCCCTGTTT





AAAATGAGATTGTTCATTATAACAGTCTCATTTTGGTTTTAAAGACTTAAC





TTCTGCACATGTAAAAACTTTAAACTCAGTTATATCAACACTTTCCTGTTC





CTCTCACTCAAGCCCAGGGCTTGGAGTGGGGTGGGGAAAACAGGCAAAAGA





AAACTGGTTGGTGAGTGAACACACTACATTGGCTACGTGCCCCCTGTTTTT





TTCTGATCTTCATATAGAAATGCTTGCTTTCCTCTCTTGGGCAGCCATCAT





TCAGAAGATCTCTGTGTGTTTGACACAGACTCCAACGATT 






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene C6. In some embodiments, the sequences from gene C6 comprise or consist of:









(SEQ ID NO: 606)


AATCTTTCTAGCTACATCAATTTTTTATTATATGCTTCTCTTTGATAGCCC





TAAGAAAGGCAACAATTCTATATTTATATTAGTGTGCTCATTTGATTAATG





TCTGTCTGCACCCACAAGACTATAAGCTTGGTGAGCACAGGATCTGGGTCA





GTTTATGCTCAATCTACTCTCTCCAGAACCTTACAGAGTGATGAGAATATC





ATGTATACTCAATAAATATTTGAAAAACAAATGAACAAAAATTTCAACAGT





TCCATTTTCTTTAGTTATCTGAACATAATCGCAATTTTAGGTTGGAAAATG





ACACCTCATGTGCACTGGAAAGGACCTTTCAGGTGGAATATTTAATGACAA





TGTATTTAGAGAAGCCAAAATGAATGATCTAACTCCAAGAGATAAATATGA





ATCCTTTACCACTGCCTCTTCTCTGAATTCAAGCTTATCACTTAGAATCCA





TGCTGTGCACTGTTCTTTTCTCCTTTTTTCTTTTGTTTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MRPS27. In some embodiments, the sequences from gene MRPS27 comprise or consist of:









(SEQ ID NO: 607)


GCAAGTGCCCATTCCAGAGACTCACTCACCTGACATATTACTACTGGAAGT





TGAAAAGTCCCCACATTTATTTGTCTGAACTGCTTAAAAAGTCACCAACAG





GAATTCTTCTCAAACTTGCCATCCAAAATTGAACCCCGCTGGCCCAGTGTT





TTGACTTGTTTATATTCCAGATAGCCACTCACGTAAGTTTATCTTTTTAAT





GCTGATGGGGGCCAAGAAAGGAGTTCATTTGCTTCCTGGCTGGGAAATTCA





GGAATTGCATTTTGGGCAGCATATTTTATAGTATGTGTGTAAGTATTTCAC





TGCTTTTCCTCAGTTTATTTTTTGTGAGAGTTCATTGAAAATGTCCAAGGC





TGGGCACAGTGGCTCATGCCTATAATCCCAGCTCTTTAGGAAGCCGAGGTG





GGAGGATCACTTGAGGCCAGGAGTTTGAGACCAGCCTGAACAACATAGCAA





GACCCTGTCTCTACAGAAATAAAGAAGGAGGCAAAGGCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MRPS27. In some embodiments, the sequences from gene MRPS27 comprise or consist of:









(SEQ ID NO: 608)


CTAGGCAAGCTCTGTCTTCACCTGAAAATCCTACTCAACACTGGGGACAGA





GATCTCTTTAGCCATGGGATACCAACTTTTAGAACTAGAGCTAGAACTTGA





GAGTACACTTTGTCACTCTTTCTTATGCTCCTCCAAGAAATACACATTTCC





TCAGAATTGTAAGTGTAATCACATTTTATAAGCTGAAATCTCAGAGTACCT





TTAGCCTTCATGAGCACAACCTTGTTGGGGATATTTGGGTTGTATTTGTTT





CTCTTTTCTACACTCACATGTTTGCCTATCTTGACAGCCTGTTGGCCTAGG





CAATAATAAAGCTTCTGTCGAGGTGAGCAAACAGCTGATGGCATAGGCGCA





CAGAGGGGACTCCTACAGTGGCTGGCTGTAATCTGCCAGGCTGGTGGTCAG





AGCAGGCGAGAACAAAAGCCAGTCTGTTTGCAGGCAAATTGGAAAATCAAT





GTGTTAGTTGTATTCTTTTCTCTTTGCCTTTCTTCTGACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene DMGDH. In some embodiments, the sequences from gene DMGDH comprise or consist of:









(SEQ ID NO: 609)


GTAAGCATATCATTTTTTTGAAATTAGTGTTTTTTAAGTGCCAAAGAAAGA





AGATATTTAGCCGTAATGTTTCGTACGATGGACATTGTCATGAAGCACTGT





GATGCTCCCAAGTTAAACATTTTGTCTGTTTCTAAGTGACAAAGCCCTCCC





TCTATCACTTTGCTCTTGGTGGATGCGCTATCACTCTGTCCTTGGTTATCC





AAAAAAAGTTGCTTATTTTCTCCATTCTGCATTTTATTTTGCTCCAGATTG





TGTTTTTCCAAATTATGTTTATTTTTCCTTCCTGTATTCACTGTTTCTCTA





TATGAATAACCAGATTCTAGTGTTGAAGTTCATAAGCATCCTCTTTGTGTC





CAATTTGCTTTTTAGGGCAGATTTGATCATTTGGGTTCTCTCTCATCCATT





TCTGATGACTTTTGACTCTAGTTGAAAAAGTTTTTAAATGCTTTTCCTTCT





AGAACATTATTAAACATATTTCATAATCTTCTCTTTTTGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene DMGDH. In some embodiments, the sequences from gene DMGDH comprise or consist of:









(SEQ ID NO: 610)


AATAGATGAGACCCATATATAATATTTCACTGATCATTCTATCAACGTATC





CCATCTGAGTTTCATTCTTCTTTGTTTTCCCCACAGTACCTTGCCTGAAGG





CCTAAAACAAAGTAGATATGCAAGAATTAATTGTTGAATGATTGAATCAAA





CATAAATTTCCTGACACCAGATATATTATTTAATGTGAATTTATTAACAGT





ATTAAAAATTAAATATAAATTATAATTGATATATCAGTTGGAATTAGATTT





GTCTTCAAATGTCAGAAACCAAAATAAGAAGGTGTAAACAAGATAGAGGTT





TATTTCTCTTTCTTTTCAGCAGTCCAGGACTTGAATGGTGGCTTTTTCATG





AAGTCTTCAGGGACCCAGTCACTTTCCAGATCAAGAAACACTTTTGAAGTC





CAGTTTTACTAAACGTTTACTAGCAGGATATATTTTTACTGAGTAATAAGT





ATATATATATTTGAGTATTGACTCTGGTATGATTGATTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ZCCHC9. In some embodiments, the sequences from gene ZCCHC9 comprise or consist of:









(SEQ ID NO: 611)


GAATGCTCAACGAGCACTGAAGGGGGGACATGAGGCACTGCCTCTCACCCA





AGAGAGCCCCCAAATGTCCTTTTCAGACTTTTATCACAGTGACCCACTCCC





ACCGCGCCTTTACCTTGCCTCTGCCTGGAATGTCCTTAACTCCCTCTTGCC





GGCAATTTCACCAGATTTTTGATTTCTTATCCAGTCTCACGGGAAGCCTTT





CCGGCATCTCCCACCCTACCGATCTTCCCGTCTATCTCGCCTGCTACACTG





TGAGTTCAGACAGCACGGATTGAACTCTTCATCTTTTCGTGTCGGCGTCCT





GCACAGGGCCATCCCATCACTAAGCGCGCCACAAACTCAGCCCAGTATTGA





CGCCCACGAGTCGCTTGTAAAGACCGTCTGTGACGCCGGAAGTGACGCGCT





CTCGGGCACTATGTGCTTCCGGTCTCCAATACCGCAGGAGGGCGGTCTTCC





CCGGCTCGCCAACTCGGCTGCTCTGGGGGATTCGTGCGCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ZCCHC9. In some embodiments, the sequences from gene ZCCHC9 comprise or consist of:









(SEQ ID NO: 612)


TGAGGTTCTCAGCCACCAAAGCTGCAGGGTCTCGTCGTTCGTTCCCTCTGG





CCTGGCGGAACTTGCGTGGGAAGCTGGGAGGACAGAAGGCTTAGCCGCTGG





GTCCCTGAGCTGGCTGGAGCGCGGGCTGTCTAGGGGCGGCGACCTCAGCTG





TTGGGCTCCGGCTGCTGGGGGCTGTGCGTCCAGCCGCCCTGGAATCCGCCT





GTGGGTGGGCCGCTTGGCTCTCCCACTTCACCCTCTTGATAACCCAGAGCC





TCCCCCTTCCCACGTACGTAGGGGAAGGATAATCTACTTCCTTTTGAACAG





CTTCGGTCCCCACTATGACAGTCGCATAAACTTCAGCGAAGAGTGCGCTGG





TAAAGTGGCATGGAGTTTCTCACACCAATTCATTGGGTCCACCCTTCAGCT





TCTGTGGAAGGGGTCTTTCTGTTACTAGGAGCTGAGACTTGGGGGGTTGCC





AGGGATGCTGGTCTTAAAAGGTGATAATTTAGGCCGGGCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene LEAP2. In some embodiments, the sequences from gene LEAP2 comprise or consist of:









(SEQ ID NO: 613)


GACGATCTGGGGAACTGTAGGCCTTGCTGAAGCATCCTCCCACAGAAAGGA





GATAAGGGCCTTATCCAATTGGCTGTACACAATAGATCAGTGCTTCTTTCC





TAGACAAGGCTGAAAGGGGCCAACATTATTTCTGAAGACTTCATTATTGGA





ATTCTATGGGAGTGATCTCACTGAGCTATTTTGGAATAGAAATGTGGCTAG





TTGCCTGACCTCCCTCAATGGTTTCACGTGGCTTTCAAAGGGAAGGAAGGG





CAGTGCTGACTTTTGGTAAAATGGGCGAAAGGGTCCATGCCAGCAACACAA





TCACTCAAAGTCCAGATGAGGGATCAGTAAATACAACGTGCCTGAAAGGTG





GCCCTTGAGCACATTCCTCCGGTAGACATTAACTTATTAAATTGATTCTGA





TTACAAATATAAACTTTGCCCCCATCTCACCCAGTAACAATGCAAGAGTTG





ATGTCAGTCTATAAAAGGAAGTAGGAACTGTCCCTGGCTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene LEAP2. In some embodiments, the sequences from gene LEAP2 comprise or consist of:









(SEQ ID NO: 614)


TAAGGAGGGAAGGATACTTATGTGTGTGTGTGGAGTGTGGAGATGATAGTG





GTGGTGGAACTTGAAAGCTAGATTCAGTCCTGAGGAATGGTTCCTCTGTTC





TGAGTCTACAGCATCTGCGGAATGGAATGATCACTCTTCCAAGGTGTGCAG





CAGGGTGTCAACACTTTCATATCTGAATGTCTTTGCCCTTACAGATAGATG





GCTCCCCAATACCAGAAGTGAGTTCGGCAAAGAGAAGGCCACGGAGAATGA





CCCCATTTTGGAGAGGGGTTTCCCTCAGGCCTATTGGAGCCTCCTGCCGGG





ATGATTCTGAGTGTATCACAAGGCTATGCAGGTACTCCCTGAACCTGGGAG





CAGGGTTGGGCCAGAGAGCCCTGGGAAGCTGGGCAGAGGAGTGACAAGGGG





ACACATGAACCTAAGAATAAAGCTGGGATGGAGGAGTTCTAGACTGAGACT





GGGAGCTCCATGGAAAATCCCTTAGGAGTTAGGAGCCAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ETF1. In some embodiments, the sequences from gene ETF1 comprise or consist of:









(SEQ ID NO: 615)


GTAAGGGGCCCGCGCTCCCCGGTTTAGATCACTCGGCCCCCCTCGTGGTAG





GCCACCGCTACTTTTGCACCTCTCCCGGGCCCTTAAGTCCAGGTCCTCCCT





CTTCGGGCTCCAGGTGCTGCCACCCGACTCGGGTGCTATTGTGGCAAAGTG





TTCGGCCTTCCTTTCCCAGGGCCCTTCCCTGATTTCCTCTTCTCCCTTCAT





TTCTCAGCCAGGCCTCATTAAAATGGCCTCTCCCTTCTGGGCTTCTGAGAC





CCGGGTGGGTGAAGTTTGGATGGGACGGGAAGCAGAGGTGGCCAGGGACAG





CTTCTTTCTGATTCCTGATTTCTCTGGGTCTTCCACCTGTCCCAAGATGAC





TTCTCCCAAGTCCTCTAATTACCCCGGAGCTTCCTGGTTTTAGCTGTTCCA





GGACCCACCTATAAACTGAAAATGCCAACGTACGCGCGATGTGAGGGGTGC





TGGGTAGGTTTTGGTTAATGAATCTGCATGTGTATGTCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ETF1. In some embodiments, the sequences from gene ETF1 comprise or consist of:









(SEQ ID NO: 616)


GAGAAGTCGCGGCGGACGCCCAGCTTCTCCTCACTCGCACCGGCCCCCTCC





CTTCCGCCTGCCGCCTTCCACTCCGCCCCTGGCGGAGGAAGTGATGTCACA





GGCCCCATGTGAGCGGATTGCAACACATGCAGCTGCCTGGAGAGAGGGAGC





CGGTGTCCTACGTCAGAGCCGCCGCCGCCGCGGAGCCGCCGCCGGGGAGGA





GCAGCCGCTGCCGCCCAGGACTGGGCCCTTAGGTAAGGCACAGGGAGGGAA





AGGCCGGTGACCTTTGTGGCGAGCTGCACCGGACGGGAGGAGAGGGCCTCT





GGGGGATGGCGAGGAAGGGGGCCGCTCTGCCCCAGCGGCGGCGCTAACTCT





GTGGTCTTGGTGTCTCCGGGCCCCGCCGGGCCGCTCTAGGGAGGAGGAGGC





GAGAAGATGGCGGACGACCCCAGTGCTGCCGACAGGAACGTGGAGATCTGG





AAGATCAAGAAGCTCATTAAGAGCTTGGAGGCGGCCCGCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene RNF14. In some embodiments, the sequences from gene RNF14 comprise or consist of:









(SEQ ID NO: 617)


CATTGCTGTCTTTTATTACATCTTTTTATATTAAAAATAAATAAAAATGTC





ATAAATAGTAATTGTGTAAATATAAAAGGCCTTATTAAAAAAACAAATCTT





AGTGCCTCAGTTTTCTCTTCTGTCAAGTGGGCACATAAAATTACCTAAGTC





ATAGGGTCCTGGGCATTTAAATGAGAGAATGTACGTGAAATATTTAGCACA





GTGGAGGGCTAGGCACACGGGGAGACTGCAGTTTAAAAGACTAACCAGGCA





CATGAGACCAAAGACACAAAAGTCTGTTATTTTCCTAGAGTCAAAAACAAA





ACTTATTAGAAGGCTGTCTTTACCCAATCTTGTCCTCAAAATTGACTCTAA





ATCTTTAGTTCACTTTAAACATTCAGTAAATTATTAACACTGCTTTGAAAT





TCTCTTTCTATTCTCTCTATCCTGTGACCTCTGACATGATGTCACAATTCA





CCTTCTGCTCTGACTTTGTGAGGATTTCCCAAGCTGGTTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene RNF14. In some embodiments, the sequences from gene RNF14 comprise or consist of:









(SEQ ID NO: 618)


TAGGGTTGCTGTGTGTTTTGCTCTTGGTCTAATTCCTTTGGCTAAACATGA





ATAATTATATTAATGAATGGCTTTGGAGATTCTTCTTCCGTCCTTTGCATT





TTTCCGAGATATGTTTCTGGTTTTGGTATCTATTAGAAGGGTATTTTGGTT





TCTGTTTTGTGACAATCAAGATTTATAGTACAGAAAATTAGAAGTGATTAT





TTCTATTTCAAGGTTCTTTCTGTGGCAGCCAGGTAATCCCTTGGGTGCCCT





GATTTACTCACTTGGTTTTTTTCTCTCTCCTGGGTATAAGACCAACTACAT





AATTTACAAAGCCCAGGGCAAAATAAAAATGCAGCCTCTCATTCAGAAATT





ATTAAGAACTTCAAGAGGGTGAGAGCACAGCATCAAATCAAGAGCAGGGCA





CTTCTTTTTTATTTTAATTTTTCCATAAGTTATTGGGGTACAGGTGGTATT





TGATTACATGAGTAAGTTCTTTAATGGTGATTTGTGAGAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene NR3C1. In some embodiments, the sequences from gene NR3C1 comprise or consist of:









(SEQ ID NO: 619)


GTAGGTTATATTTTTATTCCCATTCTATAGCTGGGTAAAGTGAGTTCTAAC





AAAGTTACTTGTTAAAGGTCACTCAGAGGTCAGAGCATCAGAAAAAAAGAC





AATCACAAGGCTGATGTTGTGTGCTGGATAGTTTAAACTGAACAGGAAGAA





AACATTTTTGTGGGCTTTATCTAAAAAGAAATAGTTTGCTCTGAGTTTCTC





AGTTTCATTTATTCAGCAAGTATGTGCCAAGTGCTATTGTAGGCAATGAAG





ATACAGCAGGGAACAAAACAAAACTGACATGGAGCTTCCAACTAGAAAGGA





GAACGAGACAACGCGTTTTAAAATATATATAATGTGTTAGGTAAAAAGTGC





TATAAGGAGAGCTAAAACACAATGAGAGGCTAGAAGAGTGATAGTGGCGGA





GGGGCTTCTCTGAGGAGATGACATTTGATCAGGGGTCTGAATAAAGTAAAG





AGGCCAGTTATGTGAATATCTGGAAGACTCAAGGGGCAGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene NR3C1. In some embodiments, the sequences from gene NR3C1 comprise or consist of:









(SEQ ID NO: 620)


TAACTATAAATTGTGCATTCTTGCATAGATCCTCCTTGGGAATGAGAAATT





AGGAAAATCCAGTTGTTAAAATGAATGCCTAAAATCAAAATAAAATTTGTT





TTTCTGGCACCTGCTTGATGACACAGACTAATAACCAATGACAAAATTGCC





CTTGAACCCAAGTTTTCATTTCCTCCTATTGTGTGGTCAGGTTATGTAAGG





GTTTGCTTTCACCCCATTCAAAAGGTACCTCTTCCTCTTCTCTTGCTCCCT





CTCGCCCTCATTCTTGTGCCTATGCAGACATTTGAGTAGAGGCGAATCACT





TTCACTTCTGCTGGGGAAATTGCAACACGCTTCTTTAAATGGCAGAGAGAA





GGAGAAAACTTAGATCTTCTGATACCAAATCACTGGACCTTAGAAGGTCAG





AAATCTTTCAAGCCCTGCAGGACCGTAAAATGCGCATGTGTCCAACGGAAG





CACTGGGGCATGAGTGGGGAAGGAATAGAAACAGAAAGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ECI2. In some embodiments, the sequences from gene ECI2 comprise or consist of:









(SEQ ID NO: 621)


GTAGTGCTAAAAAGTGACGACCTCACGCGCGCCCTCCTGCACCGCTGGGCC





CGGAGTGGTCAGGATGGGCGCGTCGGGAGGTCAGGGCTATGGTTCCCTGTC





AGCAGTCCCCAAGGTCTCCGCCTGCCTCCGCGCACGCTCCCATCCTCCTGG





GGCCGGACTCCGGGCGGGTGAGCCGCCCCAGCTGAGCACCCGGGCCTGCAC





TTCCCAGCCGGGCTGCTTCTGGGGTGCAAAGCGTCGCTTGCTAAAAGCGAG





GTAAACGCAAGCCGTTATCACTCGTGTGGACTTAACACTCTGCTTCTTGGA





GAACACGTATGCTATAAAGTGAAATGGCCGGGTACAGAGTAACGGCTAAGA





AGCACGCTGCACCTCCCTGGGGTCCCGGGAACCCGCACGAATCTGTGCATA





GGGAACAAAACCGAAACACGGTGGTGGCCTTGAAGTGGTAGAATTATGAGT





GATTTTCCCGACTTTATGTATTTTCTTAAATGGTCGTGTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ECI2. In some embodiments, the sequences from gene ECI2 comprise or consist of:









(SEQ ID NO: 622)


GGCCCCGCCCCCGCATCTGAGCCCCGACTCCTAACCGCCTAGAGCTCCCGC





CCCGCCCACGCACCGACCCCCCCGGCCCCACCCCCGAGCCCCCGCAGCCCC





AGAGGAGGCCACGGGCTAGCGATGGCGAATGCGGCGTGGCTACGCTCTCAG





CCTGGCCCCGCCCCAACCCTGGCCCCGCCCATGCCCCAGGCTCCTGCCTGG





GCTCTGCTCTCGGCCCCGCCCCGCACCCAAGCCCCGGCCCCACCCCCGAGC





CCCCGCAGCCCGGGAGCCTAGGCCCCGCCCCCGAGTCCCGGCTCCACCCCC





GAGACCCGGCCCCACCCCCGAGCCCCCGCAGACCGAGCTAGGCCCCGCCCC





CGAGACCCGGCCCCACCCCCGAGCCCCCGCAGCCCTAGAGCCGCCCAAGGG





ATGGCGATGGCGTACTTGGCTTGGAGACTGGCGCGGCGTTCGTGTCCGAGG





TAAGCTTGGAGTCCCGGCCGTTCGGCCCATGGTCGCCCGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ALDH5A1. In some embodiments, the sequences from gene ALDH5A1 comprise or consist of:









(SEQ ID NO: 623)


TGTTCTTTGTGCTTCTTGTATTTGGATGTCTAGGTCTCTAGCAAGGCTGGA





GAAGTTTTCCTCGATTATTCCCCCAAATATGTTTTCCAAACTTTTAGAATT





CTCTTCTTCCTCAGGAACACGTATTATTCTTAGGTTTGGTTGTTTAACATA





ATCCCAGACTTCTTGGAAGCTTTGTTCATATTTTCTTATGCTTTTTTCTTT





GTCTTTGTTGATTGGGTTAATTTGAAAACCTTGTCTTTGAGCTTTGACGTT





CTTTCTTCTGCTTGTTCAATTCTATTGCTGAGGCTTTCCAGAACATTTTGC





ATTTCTATAAGTGCACCCATTGTTTCCTGGAGTTTTGATTGTTTTTTATTT





ATGCTATCTATTTCATTGAAAACTTCTCCCCTTATTTCTTGTATCTTTTTT





TTTTTTTTTGTGACTTCCTTAAATTGGGCTTCGCCTTTCTTTGTTGCCTCC





CTGATTAGCTTAATAAATAACTATCTGAATTCTTTTTCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ALDH5A1. In some embodiments, the sequences from gene ALDH5A1 comprise or consist of:









(SEQ ID NO: 624)


TGAGCTCATGTGATTTTTGGGGGGTGTTAAAGAATCTTGTTTTTTCATATT





ATCAGAGTTGGCTTTCTGGTTTCTTCTTATTGGGGTAGGCTCTGTCAGAGG





GAAGGTCTAGGGCTGAAGGCTGTTGTTCAGATTCTTTTGTCCCACGGGATG





TTCCCTTGATGTAGTAGCTCCCCCTTTTCCTAGGGATGTGGCTTCCTGAGA





GCCGAGCTGTAGTGATTGTTATCTGTCTTCTGGATCTAGCCACCCAGCAAG





TCTACCAGGCTCCAGGCTGCTACTGAGGGTTGTCCGCACAGAGTCCTGTGA





TGTGAACCATCTGTGGGTCTCTCAGCCGTAGATACCAGCACAGTATTTGGG





GTGTCTCCCAGGTCCTGCAGGAGCAATCTGCTTCCTTCACAGGATCTGTAT





GCTCTCCCAGTTTTCCTGATTTATTCCTGCAGTCGTTCTGGAGCAAAAGTT





CACAATGCAAGCCTCCACATGCTGTTTTGTCCATCCGAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene APOM. In some embodiments, the sequences from gene APOM comprise or consist of:









(SEQ ID NO: 625)


CAATCCACCGTGCCCCAGCACCTGCATCATACCTGTTCTAAAGCATTTACC





AAGTTGTATTGCAATGGTTTGTCTACACAGCTAGTTTTCCCTCTAACGACT





TCTTCAAGATCAGGGGCTATGTCTTATTCGTTTTTTTATGTCCCCGGGGAT





TAGCTAGTTCTTGGGAAACAACTGGGACTTGGGATTCAAAACAGTTTGCTA





AGTGAATGAATGAGAGGCCCAGTCAAGCTACTTCCCTTCAGTGCTGCACAG





TGCAACAAATAACCAGCTCAGATACAGGTTCAAAGACCACAGGCTTCTCCC





TGGACAGCTCAGCTCTCCTCATAACTCCTGAGAAACCCTGGACATGCCAGG





GTGTACTATGGAGTGGTTGCATCCGGAAGAGGGGGAGGAAGTCCCAAACAC





TAAGTAATCCAGGTTTGGGTTGGAAAACAAGGTTGAAGTTACTCATTAGCA





GGTGAAAGGGTCAAGGGTCGAACGCAAGGGAGCTGAAAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene APOM. In some embodiments, the sequences from gene APOM comprise or consist of:









(SEQ ID NO: 626)


TATGGACTGAGATTGGGGGAAGCCTATGGTGGAGGCTCTGAGGGACTTG





GGTGGATGGCCTAGGATGACTGGAGACCATCTTGGGAAAGGAAGAGAGG





AAGGGGGTGTGAGTGTTGTGATAATGAAAGCAAGAAGAAAAATATCAGT





ACTGTGGCCATCAATGCAGAGGCATGGCAGAATTGGGGGGTGGGGTGGT





TACCCAGGTTGACTGGGGAGGGGCAAAGAGGAAAAGTCATTTAATGACT





CTTTGTCATGGATCCAATCCCCAGTTGGAAAGAGGAAGGCAGCCAACAC





CTCTACCCCTAAATCTTGCTGTTTTGACTGATGAAGAGGTTGAACCCAT





CCTGTGCTGGAACCCACCCTCTTTTGCTCCCTTCATTGTCTCTCCAGTT





CCCAGAGGTCCACTTGGGCCAGTGGTACTTTATCGCAGGGGCAGCTCCC





ACCAAGGAGGAGTTGGCAACTTTTGACCCTGTGGACAACATTGTCTTCA





ATATGGCTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene FKBP5. In some embodiments, the sequences from gene FKBP5 comprise or consist of:









(SEQ ID NO: 627)


GTAGGGACCCCATGGAGTACGTGACGCCTGGCCCTCCAGCTGCATGCTC





CAGTTATCAGGACAGAGACCCCTCCCCACCCATTCCCAGGCTTCCCTGG





CTCCCTGCTCTGCTGCTGGGAGGCCCTGTCACCACACATGCGCGTGCAC





GCACTAACATACACACACCCCTCTCAGGCACCCCCGGAAGACCAGCTGC





AGCTGGGGGTGGGGGTGCACGGAGCACAGCCAGGGTTCTCAGATCCCTA





GCCTGCTCTCCCACCCCGCCCTAGCCCCCAACTGCCGAGCTGCTTCTAG





TTTTAAAGCAAAACATGACTCTGTCCTTCCTGCTGCTCAGTGGCAGCAA





CACTGCCTGGGGTTATTTTTAGCAGGGATGCAGGGAAGGCACAGTTCTC





CTTGACTTTAACTAGGGACAACCCCCACCCCTCAGCCAAGACTCACTGG





AGGAGGCCGGGTACGGAAACCCCCCTCCCAGAAAGGGGAAATGGTGTGT





TTTCTGTTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene FKBP5. In some embodiments, the sequences from gene FKBP5 comprise or consist of:









(SEQ ID NO: 628)


GGACAGTCATCTTGGACATGCTCTGTGTTCCATATCATGACTTCAAGCC





TTGCCTTTTTCATGTACTACCAGTGGGATCCTAGACTAGCACTTTACCA





CTTTAAGCCTCAGTTTCCTAATTTGTAAATAAGGAGTAATTGTAATCAT





TCCAATATCACAGGCTTGCTGGGAGGATTAAATGAGATCATCCACATCA





GGGTCAGCACAGTGCCCGGAATGGGGTACCTGTGAAATTAATGGTGATT





CTTCTTTATTGTTACCTTGGAAAATGGAGGCCAACTGCAGGATGATCGT





GGCTCCAAGGAGCTGGGGACCCAAGACATGCACAGAGAAAGAGAGACCA





ACAATGTGAGGCCTCTGATGCATGAGACAGGCAGGGGAGTAGTGGGAGG





AGAGAGCAAGAGTGAGCAGCCAGGAGGGCCTCTTCCCTTCCCTTTACTC





AGCCTGTCACTCCTCTGCTAGGATCCTGCATTGACACATCTTGTACTTC





CTGTTTCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SLC22A7. In some embodiments, the sequences from gene SLC22A7 comprise or consist of:









(SEQ ID NO: 629)


AGGTGTGTCTTAGGCCAGGGCATCAGCTCCCAGCAGGGGAGAGGGATGC





GAGGAGGCCTCATGGCTCAGCAGAAAGAAACCTGGTTAGGATCCAGCTT





TCAGTCCTGAATCCTGGGCTGTCGGCCAGGCCATGGCTGTAGCTGCAAA





TAGGCAAGGCTGACCTAAGGTCTCACAGGACAAAGGGCACCCTGGGAGA





GCTGGCCCCAGGCCCCAGGGGCCTCCTCTTCTTAGGGATCCTGGGAGCT





CTGCTGACCAAGGGGCTAGGGGCTCACCTCCTATGCCATCACCCAGCTA





CCTCATCATGGTAGCCTTGGTCCCTGAACACTAATGGGTAAGCCCCAGA





GCCACGAGCCAGTGACCTCTGAATGGATGGCTTCCCCTTCCCCCAGGCA





CCAGGCAAGACAGGTAGAGGTCCCTGGAGACCTGATGGCTGGGGAGGGC





CCAGTCCTGGGCCAGCCCCTGAGGGCTGGATTCTGGCTGCAGGCTCTCC





AGTCCATCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SLC22A7. In some embodiments, the sequences from gene SLC22A7 comprise or consist of:









(SEQ ID NO: 630)


TACTTAAGCCCAGAAGTTGAGAGACATGTGAGAGGGATGGGCCTGCCAT





TGCCTTGGGTGGTTACTGTGTAGGCATTAGATGTATTACTTTACCTCTT





AAAGTCTCAGTTTACCAATCTGTAGATGGGGGCTATGAATATAAGGCAC





TTTTAGTTCATGGCTTGTAGTAACAGGATCCTACACAAGAACTGTTATC





ATCATCATTAATTGGGAGGTGATTAAGGCAGGGAAGGGCTAAAGTGGCT





TCAGGGAGTTGAGACAAAGAGCTGAGAAGGCAATTTCTGCAGCAAGAGG





TGGAGAAACAATAGAGGCCTTCTTTTCTCCCTTCCTCTTTTCCAGTCCC





AGGTCGGTATTTACATAATCCATCTGGAGGTGGAATGTCGGTGGAGGCA





GTCTCCCTGGGAGGCAGCAGGTCGAGGCCTTCCTTGGGAAGAAGCTGAG





GCTGCAGGACTGGGGAGGGACAAAGTTTCCTATTCCCCAAGCTGGCGTG





AATCGTTGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MLI P. In some embodiments, the sequences from gene MLIP comprise or consist of:









(SEQ ID NO: 631)


TCACTACAGATGCTCCTGTATTGTTGCTTACAATTTATATACTAAGGAT





AAAGTTACTGATATTTTGAAATAACTGTGTGTTTTCTAGTTCTTTATTT





GGACACAGTGTTTGCATGTATTTATACCTCTAAATGTTCTCTATGAGCA





TGACCAACTGATTATTGAGATTGGCAGGTGCTAGCGCCCTTCAATCAGC





TTCTCATTGCTGCACAATCTGGCTTTTCTCTTCTTTTCACAGAATATAT





AATTGGAATACATGCATGAAAAGAAAAGAGGGAAAACACATATCAGTGG





CTTTTTTTCTTTTTCTTTTTCTTTTTTTCACTTCTACTGATTCCATTTC





AGCAGTTAGTATGACCTCTCATAATGTTCCAAATCTAAAAATTGTCAAA





ACAGAAATCTACTCTTCGGAGCTGACACAATTCCTCAATTTTGGAATGA





CTCTGCTTTACCTCTTTTTAGAATTTCTTCTTTCTGCGTGAGTGTGTGT





GTGTTGTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MLI P. In some embodiments, the sequences from gene MLIP comprise or consist of:









(SEQ ID NO: 632)


TAAAAGGAAGTCTTTGCTTAATGCTTTCAGTAACTTTTAAAAGCAGTGT





ACGGTGCTGGTGGTATTTGCTGCAAGGATGTGAGGGAGAGCTGCCCAGT





GAAGATAAATTTGTATGTATATTGAAATGAAATGCTATCTTCACTTAGA





GAAAAGGGTGAGCTGATAAAGACAAAACTGCTCATTGGGCAAAGAAAAT





GCTCAGGATTGCCCTTGGCACAGTGATAATGTAAGATACCCTGTAATGC





CTTAACAGGAGGCTAGAGGCCATCTGCGAGTGTGGGTGTCAACATCTGA





AGGAAGAGAACTTTTAGGGAAATATAAGAAAGGAAGGCATTACAGCCCA





AAACTGAGCAAAGGAGCTGTTAGCCTGCGTGGCATGCGGGAAAACAGTT





TCTCAAATTATTAGGCTATCCGGTGAGAAGATGACGTTCTTAGAGATTT





TGAGCATTTCAAAGTGTATTTAGAAAAAGATTTTAATCCTTTTATCTCA





TATTTCTAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ENPP1. In some embodiments, the sequences from gene ENPP1 comprise or consist of:









(SEQ ID NO: 633)


TAGGAGCAGCGTGCCTATGGAAAATAGAAAATAGGTTCTTTTTGTTTTT





GTTCTTGTTTTTTGCTTCAGAAATAATTATAGAAATCTGTGTTTAGAAG





CAGTCCTAAGAAGTTACATGCAAAGAAGTACCAGTCTTGGAATGAGAGT





GAATCATACACATACACTCCCAGTTAATATCCTAGGTGGTTTGGGGCAA





ATAACTGGGCTTTCACATTCGGTCTAAAAATGTGATAAGACATTCTTCT





TTGCTCACATAATGATATTGTAATATATAAATAAGAAGAAACATTAACC





AAAATTTGGGTGACGGAAAAGATTTTGAAAAAAGTGAAGTGATAGGTAC





AGCTGAAATTCTGTCTTACCTATCAGATCTTCAACTAATATGAGTGCTA





CACCCATGTTTAACGAATTTAACCTTGGAAGTGAAAGAAGTTCTGCTCT





GCATATTAAATTTTTTGTTAAAGTTACAGCATGTTTTGGGATTTTTTTT





TTCTCCTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ENPP1. In some embodiments, the sequences from gene ENPP1 comprise or consist of:









(SEQ ID NO: 634)


TAAGTATATCTCTGTGATAACTTTGAATATGGTCATATTAAGAATACCT





TCCTTTAGGCCGGGCACAGTGGCTCATGCCTGTAATCGCAGCACTTTGG





GAGGCCAAAGTGGGTGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCT





GGCCAACATGGTGAAACCCTAAAAATACATATACGATACTATACTATAT





CTATACTAAAAATACAAAAATTAGCCTGGCTTGGTGGCAGCTGCCTGTA





ATCCCAGCTGCTCTGGAGGCTGAGGTACAAGAATCGCTTGAACCCAGGA





GGCGGAGGTTACAGTGAGCTAAGATCATGCCACTGTACTCCAGCCTGGG





CAACTGAGTGAGACTTGGTCTAAAAAAAAAAAAAAAAAAAAAAGAATAC





CTTCCTTTAAATCATTATATCGCTAAACTAAAGGGAATCACAGTTAATT





TTTTTATGATTAAATCTCCAATGCAGAGCTTTTGTACACAAATGGATTT





TATGGGACT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene VNN1. In some embodiments, the sequences from gene VNN1 comprise or consist of:









(SEQ ID NO: 635)


GTAAAGAAACAACTTGTGAAAAATTCACTAGTAAACATCAACTTGATTT





ACCTGGGAAAACTTTGTTGATGATCATTGCATAGATCCATGATCAATTC





TTAAGTTTCAGTATAGCTTATTTTTCATCTACTATGGGTATATTTACTG





GGAGAGCAAATATGAATTATGAAGTCACAGAAGTCAGAGCTAGAAAGTA





GCTTAGAAATCATCACATTCAGTGTGAACATCTCTGGTCTCTGACTCCT





CACCAGTGAACAGAAAAATATTTCCCTGTGTAGGTCTGTGATTTGAAAA





CTATATGAGTAAATGGCAAAAGAGAGTCACATCAGTTTAAGATTAATAG





TTTTCCTTTCTCATTGCTAAGATAGCTGATGAGGTTAATGTAGTAAAGT





CCTTAAAGTGTAAGCTGATTGTAATCTAAGAGGTGATATGGCAGGATTT





TAAGTGGTTTAAGTCAGGTCTCGGCTACAGAGATATTAAGTGTGGTGAA





AGCAGCACT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene VNN1. In some embodiments, the sequences from gene VNN1 comprise or consist of:









(SEQ ID NO: 636)


CATCTCTACTAAAAATACAAAAATTAGCCAGGCGTGGTGGCAGATGCCT





GTGATCCTAGCTACTTATGAGGCTGAGGCGGGAGAATCGCTTGAACCCG





GGAGGTGGAGATTGTGGTAAGCCGAGATCTAGCCATTGCACTCCAGCCT





GGGCAGCAGAGCGAAACTCCATCTGAAAAAAAAAAAAAAGAGAGAAAAA





AAAATGCTTGACTAGAAGCCCAAACCTCACCATTATGTAACATATCCAT





GCAACAAACCTGCATTTGTACCCTTTGAATCTAAAATTAGAAATAAAGA





AAAGAAAAGAAAAAGAAAAAGAAGTGACAGTGCACTGAAAAAAAAGGAA





ATTAAAATGCTTTGGAAAAGAAAATAAATTATAAAAATATAGAAAACAA





AATAAGATTTAAGGGGTGTGGGGGAAGCCCAAATAGTTGTTACTCAGCC





ACTCAGCTCCTCAGCTCCTCTTGCAGGCCCCCCTTTGGATTAAGTTGCA





TTTTTAACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SGK1. In some embodiments, the sequences from gene SGK1 comprise or consist of:









(SEQ ID NO: 637)


GTTCGTACTTACCGTACTATATTTTGCAGATGCCTCAAAGGATTTGGGG





TTACTTGGCATGGGGAAGGCACATAAGGTGGGGTGTAGGAGAGGGTCTC





TGGTTGTAGGTTTCTTAATTTAATGTTTGAAAACAAACATGCAAAAGTC





TGTGTGCAGGTTGATGTTTCTGGGCAGCCTGAGCAAAATTTGCTCTCTC





AAGAGGGAAAGGAACCAGGTGGGAGCAGAGCTAGGCTGGGCTAGGCTAG





TTGAATGGTGGGACATGACATACGGGTGGCACTGGCAATAACAAAGTCA





CATTCTATGAAGATTCCCTGCAAGAGGAAGCAGACATGGGCCAGTTACT





GTGATTTGAAATTGCCTAAACATTGCTTTAGGTTGGCATGTCAATTTCA





GGTACTAGTGTTTTTTTTGTTTTTGTTTTTGTTTTGTTTTTGTTTGTTT





GTTTGTTTTGAGACGGAGTCTCGCTCTGTTGCCAGGCTGGAGTGCAGTG





GCGTGATCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SGK1. In some embodiments, the sequences from gene SGK1 comprise or consist of:









(SEQ ID NO: 638)


AGTAGGTTGCTGGGACAGAACAGGGGGCGCCTGCTCCACCCATCACGTA





TCCGGTTGGAAGAAAAGCCAGTCTCAACAACTTGATTCTCTCGGTTAAA





TGATAAGTTTAGCCGACCATCCGTCTCCAAGTGCTGAACATTAGGGTAG





GGGAGGAAGGAGCACATCAGACGTTCCCTTTCTTTTCCAACCACAAATC





ATACGGTTGCATCTATTCTCTATTCCGCGTAGAACCCTCCGACCTGAAT





GCCACGTTCCTCCCGGAATGCATGCCACAGAGCCTCTGAGAAAATAAAT





CCTGTGCATGTGTTTTGAATTTTTTCCGGACTGATTTGTGAGGATAATC





AGTTAAGTTACATTTCTTGTGTGCGTGCTGGGGGTGTAATAAAGCTAGG





ACACTGATGACTGTAATTTTCTGAGTGATGTCGCAATGGGGAGAATAAA





TGGCCCAGTGTTGAACTTTGCAGCTTGCTCCCTTCCTGTACTTAAATTG





CCTTGATAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PLG. In some embodiments, the sequences from gene PLG comprise or consist of:









(SEQ ID NO: 639)


ATATCCCCAGAGTGGGTGTTGACTGCTGCCCACTGCTTGGAGAAGTATG





TTTAGGGGACAATTGACATGAAGTCTTGTCTTAAATACTTTTTCTGTCC





TTCTTTTCCTCCTTTCCTCCTTTCCTTTCTCACTCTTCCTCCCTTCCTT





CTCTGGCTGTGACACTAGGGACCAGGCCAGGGCAATTGGATAAGAGAGA





AGGGAAGGGTTTCTAGAAAGAAACTGCAGAGGAAAGACACAGTACAGAT





GATTTTGTGGGCCTGAATAAACTGCAGAACAGAGCTGTTCACTACCATA





GGCTGTATCAGTCTCTGCCCAAACAGCCCAAGAACATTCCTTAACTGCC





TGTTTCAAGCAAATCATGAATTTTGCTTCTTGCCACTCAGAAGTCACTA





ATTCTGAGTGGCCAAGGGTGTCAGGGAGACAGCACCAATTTCATGGCAC





AGAGGTTACCTGAAGGGGCTGGACCATATTTTCCTCTTGACGTCCTCAT





CTTTTCTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PLG. In some embodiments, the sequences from gene PLG comprise or consist of:









(SEQ ID NO: 640)


TACTCGTTCACCTGTGGTCTTCACCCCACGCTGGTGAAGATATTTGCTT





TATGTCTGGGTTTTATGGGCCATGGCCACTGCATGGCAGTGGGGAGGAA





CTGTCTATCACATGAAAGGCTCAAGGGCTTTGGGGACAGCATCAATCTT





CAACCCTAGCCCTGCCACATGCTAGCTGTGCTCTTGAGAAAGGCAGCAG





GACTCCGTTTTCTCATGTGGAAAAAGAGTTGAAATGAGGTACTCTGTTA





CTCCTAGAACTCACTTAATGTTCACCAGTTCATACACATTCATGATCAG





AGAACGATTCAGTTATTCCAGGCTGACAATTCCCCCTTCATCATAATAT





GTTTAAGAGAATCATATAAGACTATATTTGTTTCAAAGCACTTTAAAAA





CCACAAGATCGAGTTGGGTGTCTGGTGTGGGTGCCTGTAATCCCAGCTA





CTTGGGAGGCTGAGGCAGGAGGGTCACTTGAGTCCCGGAGTTTGAGGCT





GCAGTGAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ABCB4. In some embodiments, the sequences from gene ABCB4 comprise or consist of:











(SEQ ID NO: 641)



GGTCTCGAATCCTGACCTTGTGATCCGCCTGCCTCGGCCTCCCAA







AGTGCTGGGATTACAGGCGTGAGCCACCTTGCCCGGCTGATAGTG







GTTATTTTGAAGTAATGGAATATTTTATTCTAAATTTTCTAAAAT







GATTATATATTTTATAAAGTAAAGCATTCAAAAGAAAAAAGCTGC







ACAAATTACATTTTTAGCACATTAGGAATGAAAAACTGGAAAATA







CTCAAAAGGGAACTAGTTAAACAGAAGATTAGAAAATTTAAATAC







TTATGAATTTTTTTACAGCATGGGAACCCATTTGTGTTATATTTA







AAAAGCAGAACATAACTATGTAGATTAAATGTTGATTATACAAAA







TACACATGCATAAAGTTCAAAGATTCTAAGATACTAGAAATTAAA







AGTTGATTAGAAAGGTAACATTTTCAAATTTACTTGAATGTTAAA







ACTAATTTATACAATTTTTGGGATAAGGTGTCTGTCTGATTTATC







AATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene GSAP. In some embodiments, the sequences from gene GSAP comprise or consist of:











(SEQ ID NO: 642)



GTACGCAAGTATTTCTCACTTTTATCATGGTGTACAAAGAGTGGA







AACAACTCTGTTGTAGCAAAACCCTTTTGAAATGCACTAGTTCAA







ATCAGTTCTCACCAGCTTCAACACCATCTAGCTTAATTCTATACT







CAGCATTTAAAGTTATGCCCCCCTGCATATGTACCCTTTAATGTA







TAATAAAAGCTAAAATTAAAACAAAGTTATGCCCGTTACTTTACC







CATCCTCTTTCCCTGGCACATGGTTGGTGACAGGGGAGGGACAAA







TGAGCCCAGTACTGCCAAGAAGGGGAATTTCACTTAATACAATGT







GTAACATATGTTCCTACTCTGATTAAATTCTAGGCATTAGGCCGA







TGGTCACATTCATATTTATGGGGCTTTCTACAAGGCATAGCATTT







CATGATTAGCATGAACCCTTGTGTAGTACTCAAAGCCACTGCTTA







GGACAAATGGAGCATCACAGATGAGCTCCTTGGCTGACATGCTGC







TTTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene GSAP. In some embodiments, the sequences from gene GSAP comprise or consist of:











(SEQ ID NO: 643)



TGAGGACACTGCAATTTGGAGAGGGTGGGTAACTTGTGTAGGGTA







ACACAACTAGTAATGTATAGAATCTGGACTTGATACCAGTCTTAC







TTCACAGCTCATGATCATGACCTCCATTCTGTGTCTCTTTTCTTC







TGACTGTCCACTGTTGGCTCTTGCGTTGTGACAGGCCTGGAGCAA







ACCCTGTAGCACTTTACCTTCACTGATGGATGGGGGTCTGTAAGT







GTGAGTGGTGGGGCCAGTTAGCTGGGCTGGGGAGGCTGAGCTGGG







GCACTGCCCACACAAACAGAGGATGCTTTGTTAGTTTCTGGTGCA







CATTTTCAGTCTTTCTATTTTGGGTTCAGTGCTTCAGAATTGACC







TCAGAAAGGCTAGTTGTGTGTCTGGATGCAGCCTGAATGCCAGCT







GCCCTGAAATGCTTATCTGAGTTGTGGAGGCCTAGCTGTGGGGCC







CTTGAGGGAAGGAATGGAGCAATGGTTAATGGACTGTCTTCACCC







TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ABCB4. In some embodiments, the sequences from gene ABCB4 comprise or consist of:











(SEQ ID NO: 644)



ATCTGACTTGTAAAATAAGTTTCTGGTTTGTCATCATAAAACATA







GGTATGTCTGTCTCATTCTAGATCAATTTAGAAGACCCAATTTGG







GGTTTATTACGGCATTTGAAATTTGAGAGTTAGAAGTGACACCTG







TCTTGATGAAGTATTGAAACAACAGGCTAGCTCTCCCTAAGTTAT







TAGAAACATCCAGAAAAAGAACAAAATTCAAGGTAAGTACAGGTG







TAGAAATATAGAAGCCACAGACATAGGGAAATGGTCTTATGGTCC







TGAATGGGTTGGGTAGACAGGAAAGTTTGGGAATAATTTTCCCAA







GTACAAAAGAAAAATAAATTGTTCAATAGCCTCATCTCTCTTGGT







AATTTTCTAAATCATTCTATTGTGGAATCAAAGTTTTTTAAATGA







ATGAATTTCCAGCTTTATCATCCAACATCAAGAGAAAATGAAATT







GCCCTACCGAGCTAACTCTGAAAGCACATATCGGTTCCATAAAGC







AAGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene STEAP1. In some embodiments, the sequences from gene STEAP1 comprise or consist of:











(SEQ ID NO: 645)



AAACTCTGTGTTGAAAAAGTCATCTATTTTAAAATTTTTATTATA







ATTACACTAGGGCTTGGTTGTCAGTCGGTTTTATATTGAGAAATG







TTTTTTAGGCTGAAAATAAGGTACAACTTTAGCAACATAAGAAAC







ATTTTGTTCATGGACGAGGCCATATTTCAAACTTCTACCACCCTC







ACAAGATTACTTTTTAATATTGTTGTTCTTCTCCTTGTTTACCAA







TATGCATGCACATTACAGTTCTATCCAAGTAAACCATCAAATAAT







TATTAGAAAATTAATAGATTATGGAAGTGTTGCATTCAAGAAAAA







AAGTGATAGCTCCTATGTCATTTGTCTTAGAGGAATGCAAAAAAA







AAAAAAGAAAAAGTCATAAATTTGTGGAGACCTGTTATCAGGGCT







TCATAGTAGGCACAGGGAAGAGTGTAGAAGGAGATTCACATTCAG







GAAATAACTGTTGTTTGCATTTCTTCTTTCTTTATTTACCTTCTG







GTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene STEAP1. In some embodiments, the sequences from gene STEAP1 comprise or consist of:











(SEQ ID NO: 646)



TAAATAATATATAAAATAACCCTAAGAGGTAAATCTTCTTTTTGT







GTTTATGATATAGAATATGTTGACTTTACCCCATAAAAAATAACA







AATGTTTTTCAACAGCAAAGATCTTATACTTGTTCCAATTAATAA







TGTGCTCTCCTGTTGTTTTCCCTATTGCTTCTAATTAGGACAAGT







GTTTCCTAGACATAAATAAAAGGCATTAAAATATTCTTTGTTTTT







TTTTTTTGTTTGTTTGTTTTTTGTTTGTTTGTTTGTTTTTTTGAG







ATGAAGTCTCGCTCTGTTGCCCATGCTGGAGTACAGTGGCACGAT







CTCGGCTCACTGCAACCTGCGCCTCCTGGGTTCAGGCGATTCTCT







TGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCCATCACCA







TGTCCAGCTAATTTTTGTATTTTTAGTAGAGACAGGGTTTTCCCA







TGTTGGCCAGGCTGGTCTCGATCTCCTGACCTCAAATGATCCGCC







CACC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PON1. In some embodiments, the sequences from gene PON1 comprise or consist of:











(SEQ ID NO: 647)



GTAACAATATTTACTATTTATTAAAACCAAAACCAAACTTAAAAC







AAAAACAGAAAAACATGCCAATAAATGAATTATTTTTTCTCATTC







AAACAAAGCATAGTCATGAATAATGTTCTTACTCTGGGTTTTAAA







CTTACCCTCTATGTTAATTGAACTTTATAGTAAAACCACTTAAAT







ACTTGAGAATGCTAAAATATTTAAGATTTGTAAAGCATCTAATTT







TGTAAAATACTAACATTCACATTATCCCTATAATTACCATAATAA







TAATATCCAGCCCTGATCATTATAGCTTTTCCATTTTAAACGATT







TTCAAGACTCGGGCATTTACAGGAACAGATTTTTCTAACCCAAGG







CATCTGTAAGCTATAGGTCCAGTGACAGAAAGTCACCATGGTCAG







CTTTCTGCCGGTCCCATTATCTTCAATGCCTCCTGTCCGCTGGTT







CCTCTGTTCTGTGTTCAAAATGCTCCTCTGCATGTTGGTCCCTTT







CAAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PON1. In some embodiments, the sequences from gene PON1 comprise or consist of:











(SEQ ID NO: 648)



TCTTATATGGGAACACAATATATAAAACAACAGCTTTATTGTTTT







GATTGAAGGCTAAGAAAGGAGTAGTTCCTCTGGCCCACCAAAACT







CTGTCTTTGAGATGCTTCCAAGGACCTACCAGGGAACAGTTTAAA







AGCCATAAGCAATATTCTCTCTGTATTCCTTGCTTATATCATTGA







AGACTATTATTAATTGCCTTTTTGGTCTGCTCTTCCATTTTTGAT







CTTTCTCATGGGCTGGAAGCTGTAGTGTCGTGCACATATGACTTT







GCTGCAGGGGATGAAAGGGTTTGTGTGGGGTGGCCTAGTGGAGCA







TGTGGCTTCCAGAAGACTTCTCCTGGGGACCCATTACAGAAGCTA







ACTAAGAGGCTTCCTATGTGGCCATCTGGCTAGCCCTGTGGTTTG







GAGCAGTCAACATGAACCCATGGGCCATGCAGCGCCTCTATTAGC







ATTTGAAGTACTGGTATTGTGCTTGATTTTTCTCCTCCATGGTTT







ATAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene TFR2. In some embodiments, the sequences from gene TFR2 comprise or consist of:











(SEQ ID NO: 649)



GTGAGTGCGCGCGCGGGCGGGGGGCGCGCGGGGAGGGGGACGCGG







CTCGGGGCGCTCACCAGCCCCGCTCCCCAGGGCTCACCCCAACAC







CCTGCACTGGGTCGATGAGGCCGGGAAGGTCGGAGAGCAGCTGCC







GCTGGAGGACCCTGACGTCTACTGCCCCTACAGCGCCATCGGCAA







CGTCACGGTGAGCACCCCCTGCCCGCTCCTAGGATGGGGCGCGGC







ACGAGCCCTTTCCTGGGTCTCGAAGGAGGGACGCGGGGAGCGCGC







CTCACCGTCCTCGTGTCCCCAGGGAGAGCTGGTGTACGCCCACTA







CGGGCGGCCCGAAGACCTGCAGGACCTGCGGGCCAGGGGCGTGGA







TCCAGTGGGCCGCCTGCTGCTGGTGCGCGTGGGGGTGATCAGCTT







CGCCCAGAAGGTAAGTGTCCCTGGACAGAGGGAGGGCTTGAGCCC







GGGAACCGGAGGGTCGGACTGCCAGTGAGAATCGTTCAGGGTGCT







GTGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene TFR2. In some embodiments, the sequences from gene TFR2 comprise or consist of:











(SEQ ID NO: 650)



AGTCTCCGGCGCCGCCCTGGTGGTGATGGGAGAAAATGGGTCCGC







GGAGGGCCCTGCACTGCGGCTCGCGCCTGTAATGCCAGCACTTTG







GGAGGCCGAGGCGGGCAGATCACCTGAGATCAGGAGTTTGAGACC







AACCTGGTCAACATGGTGAAACCCGTCTCTACTAAAAATACAAAA







ATTAGCCGGGCGTGATGGCACGTGCCTGTAATCCCTGATACGAGG







GAGGCCGAGGCAGGAAAATGGCTTGAACCAGGAAGGCGGAAGTTG







CAGAGAGCCGAGATTGGTCCACTGCACTCCAGCCTGGGCGACAGA







GCGACACTCCGTCTCAAAAAAAAAAAAAACCGCCCCTGCCCCTGG







CGACCCCACGTCTCTGGCATCCTTCCCTCTTCCCTCCCTCTCCTC







CGGGCGCCCAGAAAAGTCCCCACCTCTCCCCGCTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SLC39A14. In some embodiments, the sequences from gene SLC39A14 comprise or consist of:











(SEQ ID NO: 651)



ATCTCGGCTCACTGCAACCTCCGCCTCCTGGGTTCAAGCGATTCT







CCTGCCTCAGCCTCCTGAGTAGCTGGGATTTCAGGCACGCGCCAC







CACGCCCAGCTAATTTTTTGTATTTAGTAGAGATGGGGTTTCACC







ATGTTAGTCAGGCCGGTCTCGAACTCCTGACCTCAGGTGATCCAC







CTTCCTCAGCCTCCCAAAGTGCTGGGATTATAGGCTTGCGCCACC







GCACCTGGCCTACTTGTATGGTTTTTAGGCTTCCATTGCTGTTTG







CATCCGTCTCTCCATGGAAAAGGTGTTTCTCTTGGATAGCAATTT







TGATAGTTTCCCTAGGCTTCTCCGCAGCATCTGGTGAGAGATGGA







CTCAGCAAATGTTTACTGATGGGTTGGTGGATCTCAGTCTGCCCC







ATCACACCTGAATTCAGAACAGGGCCGCCCCAGGCAGCAGGAGTG







TCCCCACCCTCAGTAATAGAGGCCCTCGTCTGTTCTTGTTCCTCC







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SLC39A14. In some embodiments, the sequences from gene SLC39A14 comprise or consist of:











(SEQ ID NO: 652)



TGCAGTAGAACAGAAACTTGCGCGTCCTCTTTCCCTGGGGCGGCA







CAGCTGAGCCAATCCCCTCCTCCCCTACCCTGGGCTCCAAGGCAC







CCAATGACTGCCACCTGAGAAATGAACCTGTCCCTCGTATCAAAG







CCACAAACGACTTCTAACTCTGGATCTCAGGGCCCACCTCTCACT







CCTCTGGCCTCTGCAGGTGCTGTCAGATGCAAGGTTCTTCCAATT







TCCCTCCTCCTGGGGACTTGACAGTCTCCCCTCCCTTGTTAAGCC







CCATACATAAGGCATCAAGGGAAAGCAGCAGCTATCGACTGCCGT







CCATGCCTTTGGTGCTAAGTACTTGCTCTATTCTAAGGAGAAATC







CTGGCAGTACTCAGGTCTGGGCACCAAGAGCTTATCCATCTGGAA







TGAGATGGAAGGAAAAGAGGTTACCAAGTGATATGTAGAATTGGC







CTAAAAAGCGAGTGTTCTTTAATTTTGAGCAGGCCATCGTTTCTA







GCAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene RBPMS. In some embodiments, the sequences from gene RBPMS comprise or consist of:











(SEQ ID NO: 653)



CCTTAGGAGTCGCCCAGCAGCGAGACAGCTGTGGATCCCCTCCAG







GCGCATGGGAGGCTCACAAGGCAAGGACTCTGATGGTTTTGGCTT







CCACCTCCTGTACGGTGGCCAGTGCTGTGAGGGTGGTTGGTCCCA







CAGGGAAACCTGTGAACAGAGTCATGTCTGGGGCCCCAGGGACAT







GGCTTCAACCCCTCACCTGGCAGTCCTGCCTCCCAAAAGTAAGGC







AGGAGTCCGGAGATTCCCATTGCTTGCTTCTGTGAATCTCAGGTC







ACTTTAGCACTTGACCCCTGACCCCAGACTGCCTTGTCCTTGAGG







CCTGGGATTCCTAAGGTATATACTGTCTGCTCCAGAGCTGTGGCT







GTGGCTATCCGGAAAACGACAGCTTTGAAGGACTGCAGAGCTTGC







CTTTGGGGCTGTTCTGCCCAAGGCCTTCCATTGTTTTCAGACATA







AATGAAATTGTTAATCCTCTGACATTTACCTTTTCCTCTCAACCT







GCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene RBPMS. In some embodiments, the sequences from gene RBPMS comprise or consist of:











(SEQ ID NO: 654)



TGAGGCTTTCTAGGAGCACCTGGCTTAGCTCTCACAGGAGGAGGG







GCGGACGGGGAGGCCAGGCCTCATGCTCACAGCGCCAGCCTCCTG







TGAGAGTGGGCTGGGTCTCCTTTCCTTTCCCAGGCCCTTCCTGGA







GGCGATTTCTTTCTTTCTTTCTTTCTTTCCTTTTCTTTTCTTTTC







TTTTCTTCTCTCTCTCTCTCTCTCTCTCTCCCCTCTCTCCTCTCT







CTCTCTCTCTCTCTCTTTTCTTTCTTTTCTTTCTTTTCTTTCTTC







TTTTTTTTTTCTGAGGCAGAGTCTCGCTCTGTCGCCCAGGCTGGA







GTGCAGTGGTGTGATCTCGGCTCACTGCAAGCTCCGCCTCCCGGG







TTCATGCCATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGACTAC







AGGCGCCTGTCACCACGCCTGGCTCATTTTTTGTATTTTTAGTAG







AGACGGGGTTTCACTGTGTTAGCCAGGATGGTCTCGATCTCATGA







CCTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene DPYS. In some embodiments, the sequences from gene DPYS comprise or consist of:











(SEQ ID NO: 655)



GTAAGTCTAAGTTCTTGTATTCCTTGGCTTCATTGTGGAGAAAAT







GCACTAACTTGCACAGAAATAATGCTTTGGCATTAATCAGCCGCT







TCTCCTTAGATGACTTCTTTAGCTGTGCTCAGGTATTTTGATCCT







GTACACAAGATAAAGGTTTTGATGTGCAAATATTCCTTTGTTTTT







CTTGGCATAGAGGATGTAGAAGTTGTGTTGCATTCTCTGACAATG







GATCTGTATATAAAACCAATATGAATTTACCACTTCAGTGCCTGG







CAAATGCCCGAGTTCACATGGAAGAAAGGTAAAGCGTGATGAAGG







AAATAAAAGGCTTAGTTTGAAAAGGGTTGAATTGAAAACTCAAAA







TAATTACAGCCTTTTTAAAGCTTTCAGATAGAAAAATTCCAGCAC







AAAGGATGTCACTTTCAGTTAGCCAAATTTTCCAGTTGAGTTCAT







CTTCAGCCTATCATTCCTAGTAGATTCCAGCTTTTTGTTTCCAAT







TTCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene DPYS. In some embodiments, the sequences from gene DPYS comprise or consist of:











(SEQ ID NO: 656)



GCACCACTGCACTCCAGCCTGGGCAACGAGAGTGAAATTCCATCT







CAAAAAAAAAAAAAAAAAGTGTTGTCACCTATTCTCACTCTATAA







TATTCTATCTAATGTCTACATGATTATCTCATAAATCTTAGGTCT







GTAATAAATAAGTCAGATCATAAATGTGGTATTGTCAAACTTTAA







AGGAGAATCGAAAATACCATTACCAAAATTTAGCTGTCACCTATA







ACAATTTTTGTGATTCTAGGATGAATGGGAAGAATTAGTGTGGAT







GCTAAGTAGTGGGGTTTCTGAAAAGTCATCTTCAGGCTTCCATAT







GTTACCCCATCATCTCCATCATTGGCATTATGACTGTTTAATACT







TACCAGCATCCTCAGATGCTCTACAATATAAGAAATTAGGTGCAG







TTCTGTTTGTTTAAGAGATGCAAGTCTTGTCATTTTATCGATAGG







ATACTTTTTAGTATTTGATCATTAGAATGATTCTTTGAATTGCTT







TACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene RCL1. In some embodiments, the sequences from gene RCL1 comprise or consist of:











(SEQ ID NO: 657)



TATTGGGATGGGTGAGAGGAGGAAGATGCTTTTTGGGAGTCAGAT







CTGTTTAATTTTGAGCAAGTGTCTTAGTTTTTTTGTCTTAGTTGT







CTTTAATGTAAGTCCTAGAACTCGGAGCCATGGCCCTCAACACAG







GGTTACTGTGAGCATAAAGTAAGGTGATAATAGATGTGAAAGAAC







TTTGGGAGCCGTGAGCTCTGTGGGATTATGTGCTAGGATGGTGAC







ATCTCTTGTGCTTTCCTGGGGACAGTGGGCAGCCTCAGACCTCAG







AAGGACAGCACCTGCTTGGTCCAAAGGGTATGAGATTCAGGACTG







TTTGTCTAAGCTGTCTGTGGTCAAAAGTACATTCTGGACTGAAGA







GATGGTGATGTAACTGGGCTGTGTGTGTATTGAAAAATCCCTCTT







TGCTCTTTCCTCATTTCAGGTTTGTCCTTTGAGTCAAGTTAAATA







TTTATTGAGCATTTCTGAGTACAAAAGTGGTGTATTGGAGAGTTG







GGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene RCL1. In some embodiments, the sequences from gene RCL1 comprise or consist of:











(SEQ ID NO: 658)



TAAGTTCAAGTGGA







GCTAACACTTGGGTGCCGGCTGGAGGGTTTCAGGAGAGAGATT







GGGACCTCGGGCTGGGAGAATGGGGCAAAGCAGCTCAGGTTGAGG







AATTTGGTCAGTTTGGATGGGTGGAAGGAGTTGATGGTGCAGAGC







TGAAGATGGACACAACTGAGCAGGCTGGGGGCTCTAACAACAAGA







TATGGGAGGACCAGGAGCCACCGAGAGACTGTCACCAGCATGAGG







AAGATTGGTCTTTTAGTATGAAGTTCAGGATGGGTCAAAAAAGGG







AGCTAGATACCAGATCAGGTCATGTATCGTCTCTGAGCACTTTGT







TTCTGCAGTTCAGATGAAAATTAAATATGTTATTGTCAGCAGTTC







TTGAACATTGGGCTGGATTGGGCAGATTATATACCTCGTTCTACC







TGTTGGACTTGTCACGTGGGGTTTGTATCAGGGTAGTGGGAAGGT







CACATGTTATTTCTCCATTTTTTTAGTTCCAAATATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene TOMM5. In some embodiments, the sequences from gene TOMM5 comprise or consist of:











(SEQ ID NO: 659)



GTAGCGGGGAGCAAGGAGGGTGCGCGTTGAAGGCGACGGGCATAT







GCGCGGTTTGAGGTCTGCGGGTCAGGGTCCGGGCACTGAAGTGCA







CGTGGTGGCCGGCGCGCGTGTTTAATCATCCTGGTTCTCCCAGCT







TGAAGAATGTTTGAGCTGCCGGGCGCGTTGGCTCACACCTGTAAT







CCCACCCAGCACTTTGTGAAGCCGAGGCGGGCGGATCATTTGGGG







TCAGGAGTTCGTGGTGGCGGGCGCCTGTAATCCCAGCTCCTCGGG







AGGCTAAGGTGGGAGGATTGCTTGAATCCAGGAGGCGGAGGTTGC







AGTGAGCCGAGATCGCGACACTGCACTCCAGCTTGAGTGACAGAG







CAAGACTCCATCTCAAAAAAAAGAATGTTTGAGCTGAAAGAGCTC







AAGTAATTATCCCTAGAGGAACTCCCTCGTTTTTGCAGACAGAGA







AACAGAGAAACAGAACCATAGTTCTAGAAGGAAGAAACAGCCTTC







TTGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene TOMM5. In some embodiments, the sequences from gene TOMM5 comprise or consist of:











(SEQ ID NO: 660)



AGGCTGGAGCGCAGTGGCGCGATCTCGGCTAACTGCAACATCCCC







CTCCTGGGTTCAAGCGATTTTCCTGCCTCAGCCTCCCGAGTAGCT







GGGATTGTAAGCACGCGCCACCGAGCCGGGCTGATTTTTGTATGA







CAGATGTTATTAAAGACAGCGTGGAGAAACCAGAGCCTGCGTTGC







TGAGCTTGCGGCCGCCCGCTTCACACCGCACTTCAGATCAGCTGG







GTCAGGACGTGGATTGTCCCGTGCAGGCATCCATCGTCGAGTGTG







GCAACGAGAGGGCGGGAACAGGAAGTGAGGATAGGCCGAGTTCCG







GGCGCGAGGCGGCCACCGTGGAGAGCAGAGCGCGGCGGCTGGAAG







CTGCTAAGTCAGAGCCGCGATGTTCCGGATTGAGGGCCTCGCGCC







GAAGCTGGACCCGGAGGAGATGAAACGGAAGATGCGCGAGGATGT







GATCTCCTCCATACGGAACTTTCTCATCTACGTGGCCCTCCTGCG







AGTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CBWD5. In some embodiments, the sequences from gene CBWD5 comprise or consist of:











(SEQ ID NO: 661)



GTAACTAACCATCCCAGTCACAAAATGCCGTGAACTCTGGTGTCA







TGGGATTTTGCGGGCTGCCGGTGCCTCTTCTCCTGAGGCATTGGG







GCCTCATGATCAAGAGCAAATGTCTTTGAGGTAGGGGCTGGGTCA







CTTGCTTCTCGTATGGGTTACCTCTCATCTTGGGAGTTAATGATG







CCCGGTCCCCTCTGCGTTAGATTCCCATTTCTCAAGTGAGATGGA







AAGTTAGCCTTTGAAACAAGGCTTTGAAGCTAAGACACGCCCCCC







TAATTGTCTCTGTGACCTCTTACCAAGTTATCTTACCTTCCTGAC







GCTCATCTACCTCACCTGAAAATGGAAAAATACCTGTCACCTGTG







AAATAGGGCCGGAACAACCTACCTATTGCCATTAAAGGATGTCCC







GTCTGTAAAATGTCCGACACGTGGGGCTTTCGTCAGCAGATTCCC







CTTCCCCAGGAGCCAAAGTCCTCCAGCCAGTTGGCAGGAAGTGCT







CCTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CBWD5. In some embodiments, the sequences from gene CBWD5 comprise or consist of:











(SEQ ID NO: 662)



CTGAGGTCCAAATAAATAATGAGGAAAAAGTAAATAAAAGTGATG







CATGAATACTAAAAATTTGGAATTACATATATAATATGTAATAAT







AAAAAAAGAAAAAATTGTCACTGGTTATCCTTCAGGTATAAAAAT







TACAATTAAAATTGTAATGTTATAACCTTCCATTCTTTCCCCTAT







GAATTAAAACTTTTTTTTTTAACGAGACTAAAAAATCAAGTTGAT







AAAATCTATGCCCTGTGGGAACTGGTTAGGTCAGGTTACCCACTC







CAGATTTCCAGCAGGCTAGCCTCAGCTCTACCTACAGCCAGTAGC







TTATTAAGGCTCTAGGCGCCAGTGACACCTTCAGACAGGTGGTTA







GTGCGCCCTAAGGGCTCTCCGCAGCGTCGCTCAGGTTCACAGAAC







ACGCCCAGGGGCGTGTCCAGCTGTCGTCGGGGAGAGCCCACCTCC







CCGGGGGGTGTGGCTAAGGGACGAGGCAGTTCTCGTCCAGAGCCC







AGGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene TJP2. In some embodiments, the sequences from gene TJP2 comprise or consist of:











(SEQ ID NO: 663)



AGAAAAGCAAGACAGACTGAGAAAGTGTGGCTTAGCCGCGGTATG







GAGGATGGATGGTAGCACAGATAGGCTGCAGGGCCCCTAATTTGA







GTTCCTGCACAAAAACGACCATAATATCAACATTGTACCAAAGCC







AAGTGATCTCAGGGAGAAAAATAAAAGATGCCTTCCCTGGCGAGC







ACATCAAGGTTTCAGTCGCTTGGCTGTGTCCTTGGGAATTTTCTT







GAGTCCCCACTCCCAGGAGCCACATCAGACCCCACTGACCGTCCA







ACACAAATTCCTCTAGATGGAAGGGATGGATGATGACCCCGAAGA







CCGCATGTCCTACTTAACCGCCATGGGCGCGGACTATCTGAGTTG







CGACAGCCGCCTCATCAGTGACTTTGAAGACACGGACGGTGAAGG







AGGCGCCTACACTGACAATGAGCTGGATGAGCCAGCCGAGGAGCC







GCTGGTGTCGTCCATCACCCGCTCCTCGGAGCCGGTGCAGCACGA







GGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene TJP2. In some embodiments, the sequences from gene TJP2 comprise or consist of:











(SEQ ID NO: 664)



GCATAAGGAAACCCAGCCCAGAGCCACGAGCTCAGATGAGGAGGG







CTGCTAGCAGCGATCAACTTAGGGACAATAGCCCGCCCCCAGCAT







TCAAGCCAGAGCCGCCCAAGGTACGTGGCTGGGAAGCCCAGGATG







GGAAGGAAGAGGAAGCAGATGCCTCTGAAGCCTCCTGGACGGCCA







GGGAGGAGCATGCACACTGAGATGGTGTTTAATTACGGTTCTGAC







TCACTGTGTTCTCTATTAGAGCCTATCTTTTGGCCTGTTGGGTGA







ACTCCCAAATTAGGGAAGGGCAAAAAGGAAGGAAAGGCAATATTA







GGAGAGCATATGATTTTAGGACTGTTAGTGTTTATTTAATTTACT







CTTCTTGTTAAAAAAAGATTGTAAAGAAAAAGATTGAAAAATACT







GTACTGTATACCCAGAATCACCAATCGCAGAACGTGTTTTTCTTT







TGCTGTCCAGCTAAAGTTTTCTGCCCAGTCTGATTTAGATGCACC







AGAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ALDOB. In some embodiments, the sequences from gene ALDOB comprise or consist of:











(SEQ ID NO: 665)



GTAAGGATATTGCCCACTGAGATGAATCATGCCAGTGTAAGCTAG







AAATGAAGGCAAGTAAAGAAGGAAGCAAGGAAAAACAAAACACAA







CTATTACACTGGTCTTAGAGCCACACGTACCTGGTTTTCAATACC







AGCTCCTCCATTTAACAACTGTGACCTTGGGCCAGTCACTGAAAT







ATTGTGAGCCTCAGTTCTCCTATCTCAAAATGGGGATTTCTCAAA







ATGTTGTGGGGATTAACTTAGACAATGTACATAAGGTGCTTACCA







CAGTACCTTGGACAAAGAAGATTAAACTCTGCACCCGGAAGTATT







ATTTTTAGAAAAAAATTAGCAGCAGTAGAGTGGAAAGTCCCCACA







CTTTGAGGTCAGACACACCTGGGTTTGAATCCTGGTTCTATCACA







TGCTACCTAGATAATTTTGCAAGGTACTTCTGCCCAAGTAGACAA







GCATTAACTCACAACATTGGGTTAGCTAAGCAGAATTTGAATCCC







AGCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ALDOB. In some embodiments, the sequences from gene ALDOB comprise or consist of:











(SEQ ID NO: 666)



ACTTCTCAAAAGTGAAAACTAGTATCCATTGAATAAGAGTTTTTA







CCATTCCAAGCATTCATTTAATTCTCATAACCTCTGGTAGTAGAC







TCAATTATTACCCTTATTTAGAGATGAAGTGAGAAAACTGAACTA







CGAAGAGTAACATAACTTGCCCACACAACAAGGCAAGGTCAGAGC







CAGTTTAGGCTAATTCTCAAGCTCATGACTTTTGCCTTTGCTATG







TAGAGAGTAGAGGTTGTATCCTTAATTCTCTTTACCTCCTGTACT







AACACAACACCTGGTGCATGCAGGTGCTCAATAAATGTTAGGGTA







GACACTCTCACAGTGTACCTTCTAGGTAACTTCCCTTCTGCTATG







GACTGAACTGGGTCCCTCGCACTAATACACTTTACTAATTCAACT







CCTTGATCTGTAAAAGATGCAAACTGTTAGTTAGTTCAAGAGTTC







TGCTTGTGTTTTCCTGACATCTGACACCTGTTAATTATTTGTATC







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PTGR1. In some embodiments, the sequences from gene PTGR1 comprise or consist of:











(SEQ ID NO: 667)



GTAAGTAGACCTCCTGCTTTCTTTCCTTCCCTTTTCTCCCTCCTC







TAACCCCCTTGCTTTCTTCCTGCCAAGTTTCTAGACGCCCCAGTG







GAGGAGGTTTGCTCTTAGACCTCCTCCCCTTACGTCGAAATGACC







GCTGGGTCTTGCACTCGGAGGATCTTGGGAGCCGACGTCCCGACG







CCTCCCGCCCCCGCAGTTCCTTGGAGAGCTTGGAGCCGCGCGCCG







GAGGGAATAGGAAAGCTTGGTTACAACCCGGGACACCCGGTAGCT







TCCTGCACCCGGGAGGGGGCTGGCCAGGGAACTTGCTGGACTGCA







CAACTGGGGGAGGGTAGAAGAAGAAAGATCGGGGTGGGGATTCGG







GAATGGGCAAGCGGGCGGCGGGCGGCGGGAGGCGAGGGTGGGAGC







AAAGGGCGAGCGGGCAAAGGCCGGGCGCCGCGCCAGGCCCCCTCC







TCGGGCGGGCGAGACTTTCCCGGGACCTGGCCCCTCTGCGGCCAG







ACCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PTGR1. In some embodiments, the sequences from gene PTGR1 comprise or consist of:











(SEQ ID NO: 668)



GAAGCCAGGTCAAGGCCTGATGTTTGCTAGGGTGTGATGGGTGGG







GTGCCTCAGTGGCAAGAGTGCTCACTCCTGCCTGGGGGAGTCTGG







CAAGGGTTCACAGAGGTGACCTTGAATTGGCTGGTGGAAGGCGAG







TAGGTGTTCAGCAGGCGGACTGGGGACAGACAGGCAGGGTGTCTG







CACCGCAGCCCTGGGCGAAAGCTTGCACTGCGGGCAAGCGGCAGG







GCTGACGGGGAGCCCAGGGATCGGAGCCAGCACCGGGGGCTTATT







AGGGCTGCGGCCGCCCCAGGCCCTGCCTTTGGACCCTTCCGGCCT







GCTTGGCAGATGCCACGGTTGGTTCCCCCACGGGGCATATTTCCG







CCGGGCTCCAACTTAGGACCCCAGGCGGAGGTCCAGTGTCAGCTG







GACAAAGCTCTGTTAGGAATTTTCCTATCAGGAGTCCCTAACCCC







CTACACGCACACACACGGGCGCGCGGGCGCGCACGCACACACACG







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ORM1. In some embodiments, the sequences from gene ORM1 comprise or consist of:











(SEQ ID NO: 669)



AGCCTCCTACCTCTGCTGGAAGCCCAGATCCCATTGTGTGCCAAC







CTAGTACCGGTGCCCATCACCAACGCCACCCTGGACCGGGTGAGT







GCCTGGGCTAGCCCTGTCCTGAGCACATGGGCAGCTGCCTCCCTT







CTCTGGGCTTCCCTTTCCCTGCTGGCTGTGGTCGGACCCCCACTC







CCGGCTCTGCCTTTTTCTCTTCTGGGTCCCCAGGGTGAAATTCTC







ACCAGCCCAGGGGACTCTGGAGGCACCCCCTGCCTCCAAACACAG







AAGCCTCACTGCAGAGTCCTTCACGGAGGACGGTTCTGTGCTGGG







CCTGGAGGGGCTGCCTGGGGGGCAATGACTGATCCTCAGGGTGAG







CTCCTGCATGCGCACTGCCCACCAGGGGCCTCATCTCCCCATCTG







CAAAATCAGGGAGAGATCTGCCTGAGTCTCCTCCCAGCTGACAGT







CAAAGATTCAGCATCAAGCCCCCATCACCAGCTCCCACCTTCTCC







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ORM1. In some embodiments, the sequences from gene ORM1 comprise or consist of:











(SEQ ID NO: 670)



TGAGAGCCCCCATTCCAATGCACCCCCATCTCAGCTTCTGGCCAG







AAGACCTGAGCAAGTCCCTCCTTCTTCCTGGCCTTGGCCTTCCCA







TGGGTGGAACCGGGAGGGCTGGCTTTAATCTCCACCAGACTCTTG







CCCCAGGACTGTGATGGGCGATTGGCCACTTCTCAATAATCTTCC







TGTTTTCCTTCCGCCTTCTGTTTGGCTTTAGACAGGACCAGTGCA







TCTATAACACCACCTACCTGAATGTCCAGCGGGAAAATGGGACCA







TCTCCAGATACGGTGAGGGCCAGCCCTCAGGCAGGAGGGTTCACT







GTGGGAACAGGGCAGGCCAGCATAAGGTGGGGGCTGGATGTAGAG







CCCTGGAGGCTTTGGGCACAGAGAAATAACCACTAACATTTTTGA







GCTCTAACGATGTGCTCAGAAACAACTCTAAGAGGACACTGAGAG







AATTAGAGGAGGAAAAAGAAGAACAGAGACCTCAAATAGTTTCCC







CAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ORM2. In some embodiments, the sequences from gene ORM2 comprise or consist of:











(SEQ ID NO: 671)



TAGTTTCCCCAAGGTCACACAGCTTATAATTAGAACTAGAATTGG







AACTCCAGGCTGGCTTCAGATCTGCCTCTCTCTCACGCCCTCTTT







AAGATCCTTTGCAAACCAATGGTAGAAGCCTGTATGTTGGAGAGG







TGGTACCTTCAACTATGTCCCCCATCACCGCAGAGGTGGCACATG







GCAGGGATCTGATGGAGCTGAACTGACATCATTTAGCATCCCGAG







CCTCCTCTCTGGGCCTCATTTTCCTCCTCTGTAAAACGGGGAGAA







AGGCCCTGACAGCCACAGTCTGTGTGAGGCTCCTGAGATCTCATG







TACAGAAAGTGCTTGGCGTGGAGCTGGGCACGCAGCAGGGGCTGG







GCACACGGTGGCCCAAAGGAGACCCGGGCCTTCACTGATGGGCTT







TGTGGCCCCGGACACACCTAGGACTCCTCACCTGTAAGACAGGCA







CCATTGTGCCATCCCATGTTCTCACCCAGAGGCTCTTTTTCTCTT







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ORM2. In some embodiments, the sequences from gene ORM2 comprise or consist of:











(SEQ ID NO: 672)



TAGGCATGCTTAGCAGCCCCAAACTCATGCCCCTCTCAGGCCTCA







CCCCCCATTCACCCACCCCTGGGCTGGCCCCTAGAACCCCAGCCC







TCCCTGGCCTCCCGCCGGGCCCCACCATGTCCCCAGTCAGTCTCC







TTGCTCCCCCTGCAGCTGACAAGCCAGAGACGACCAAGGAGCAAC







TGGGAGAGTTCTACGAAGCTCTCGACTGCTTGTGCATTCCCAGGT







CAGATGTCATGTACACCGACTGGAAAAAGGTAAACGCAAGGGATT







GGACAGTGCCCACCTTGTCCATGGCCCAACTTGGGCAGCCCCAGA







GGCCCAGAGCAGGAAAGCTGCCAGGCAAGGCTGCACAGCTAGGCA







GATCTTCTGCTTTTAGGCACCTGCCTCACTGTAGGGACAGCTGAG







CTCTACAGAGGCCCAGGGGTGGTGGATGAGAGCCCAGGAGGGAGA







AGTCCCTGTGAAACCAGGGAGGACCTGAAAGCTAACAGGAGGGAA







CAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene C5. In some embodiments, the sequences from gene C5 comprise or consist of:











(SEQ ID NO: 673)



GTAAGTAAAACAAGCTATCTTCATTTTTCAATGCATCTTAGTTAT







GACGAATGAAGAGTTAAATTTAACAGATTCTGTTAACTTGAAAAT







AACTTGCTACTTCTGAGAACAGCAAGTGAATAGTATCATCTCTGA







ATGAGAAAGTCATGGAAGCATTTTGATTTGATGAGTAGCTAACAA







CATGACAGCTTTTATGGAATTGTTATTTATTAAAATTTACGTTAA







GTGATTATTCAAATTCCTCTTCAAAAAAGCACAAAAGAAACTCCA







GATAATAAACTTTCTTGACATTTTTTGCTTATTTTAACTTGGTGT







TTAGTTTTCTTTGTGTTTTTCATAGACTATACACAGTTAAACCAG







GGTTATTTAGGAGTCAATCTAATACTGTGATCTGAAAATCTGTTG







TTGTAGTGACTTCTACCATACATTTATGAGCTGACTTTTCACAAT







TTTTAGTGTCATTATTGAGATAATATGCATTTTAAGTAATGTTTT







AAAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene C5. In some embodiments, the sequences from gene C5 comprise or consist of:











(SEQ ID NO: 674)



CTAGGTTATGCTCAGACCATTTGCAGCACCAAGGACACTGCCATT







CAGGTCACCAAAAAAAGATCATCTTGTGAGCCCTGTCTAAACCAG







GCCCCTTCTAAAATCATTTCAGTGTGAAACTGAGTCCAGGTGGGT







CTTCATCAGTATTAGAGTGGACCTGCCAGATGTATGCTTGCAGAT







GATGCAACATCAAAGCAAATGCCCTGGGCAAGAGAAGAAAGGCCA







AAGCAATGGGCTTACTGGTTTCTAGCTTTGAGCCACTCTTCATAG







TGCCAAATTCCCATTTTAGGGTCCCTTTATTTGTATTAATAAAAA







TAAATTCCCTGGGTGTAAGTTCCTGTAAAGTACTCCCTATCTGTA







TTCCTGGGCATGAAAGGGTGTTTTAGTTCCAATTCTAGTTTTCCC







ACTTAGAGGTTCACCTTTCTGACATTAATAATTAACCAGATTATC







TGAATTCTTCAAGTTCAGTTTATTTAAAAGGAGACTATCCTCAAA







AGTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene DMD. In some embodiments, the sequences from gene DMD comprise or consist of:











(SEQ ID NO: 675)



CGTCTTCACCTCTTTTCAACCTGCTCCTCTTTTTTGCCTTAACTC







TGATGGCAGTCACACAACTGTGTGTTTTTCTTTTAATTCACTTTA







CACCTTGGTTTGGCTATTGCTTTCCATGGTTCATACTTAATTGGC







TGGATTTTATTTATTTTGACCTTTCAGTAAATTTTTTTGCGGCTG







AGTTTGCGTGTGTCTCCTTCACCACCTCATTTTTTGTTTTGCAGT







CCCGTTACTCTGATCAACTTCTGGCCAGTAGATTCTGCGTGAGTA







CTTTTTTTGCTGAAGGGTGCTGCTACCACCAACACATTCGCTCGC







TTGGTTCTCTTTTGTTCTGTTTATTTGTTTGTTTTCTTTTGTTTT







GTTTTGTTTTATGGACATATTAATTCCCCTTCCTTCATTTCAGTT







TTTTTTTTTCTTTTCTTGATGAGACTTTGAAAGTACCATCTACAC







AGATCAGGAGAATCAGAGTGGGTTTTTTGGTTCCTCCACCAGGTG







AAAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene DMD. In some embodiments, the sequences from gene DMD comprise or consist of:











(SEQ ID NO: 676)



AAAATACCATAAAGTGTGGTAAAATTTAATGTCACCTTTTTGACC







TTAAGACCTATAAACTACCATTTATTGAGTAGCCTAGTAAGCTTG







TATGTTTCTTTTATTTATCATAACAACGTGTTGAGGTGTTGTTGC







TCCCATTTTACTGATGATCAGAGCCTCTGAGAAGTAAATAACTCA







GCCAAATTAGTACATGCCAGGCCTGGTTTCAGAGCCCCATTTGTC







TGATTCCAAAGCCTTTGCTCACAACCACAACACTCTCCTTAATTA







CAAAACAAGTGTCATGGGGCAGAAGACTGGAGTGGTCATTAGTTT







TGAAATCATCCTGTCCTAAATCTGATCTCACCATGATCTCCCTTT







TAGACTACATCAGGAGAAGATGTTCGAGACTTTGCCAAGGTACTA







AAAAACAAATTTCGAACCAAAAGGTATTTTGCGAAGCATCCCCGA







ATGGGCTACCTGCCAGTGCAGACTGTCTTAGAGGGGGACAACATG







GAAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene GJB1. In some embodiments, the sequences from gene GJB1 comprise or consist of:











(SEQ ID NO: 677)



TTGCCCAGGCTGGTCTCAAACTCCTGGCTTCAAGTGATCCTCCCG







CCTTGGCCAAAGTGCTGGGATTACAGGCATGAGCCACCATGCCCG







GCCCTGTCATACAGTTTTAGAGGCTGGGAGCTAGAAGGGTTGACC







AGGATGGCAGCCTGTACAGGATCCCTCTCTGCAGCTTGCCCGCAC







TGTGGATCTGCCTAAGGCCAGGGCATGGGGGAAGCCTGAGGGATG







CTGAGGAGCACAGGTCCAGCTCAGTCTCCCAGAGAGACTAAACAT







GGGTGGGTGACAATGATGCCTCTCTTCCTCTGGGACAAGGCAGTG







ACTCCAGGCTATGTGATCTGTCTCTCCAAACCCATAGGGAGCTCA







GAGGATCAATCTTGTTTAATGATTGTATGTGGGAGGAGGTGGGGC







TGGTTAATGTTTGTCTGGTTCCTCTTGGGCTGTGGGCCATTTCTG







GCTCTGGCCCTGGGTTCGTTGTCCCTCCCCTGGGCTGGGCTGACA







GTAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene GJB1. In some embodiments, the sequences from gene GJB1 comprise or consist of:











(SEQ ID NO: 678)



TGAGGAGACTGGGGGCTGAGGGAGGTGGGCCAGGGAGGGCTGATG







TGGCACAGTGGGCCGGAAATAGGGGCCGGGTCTTGCCTTCTACTG







ACCTCCGCCTTCAGCCACTTAGGTCCCTTCCATGCTTCCCTCTGA







AGCTGGGGTGAGGCCCCTCCTGCCAGGTGCCAGACTTGCTTAGCC







TCTCTCTGCAGCCTCTTTCCAGGTTCAACATTGCTTTTCCTCTCA







CTTTTTTTCCTCTGGCCAAAGATTCCCCTTATTCCCATAGGGCCA







GATTCTCAGGAAGACTTGAGGGACCCCCTAGGTGCAAGGTGGCCA







AGTTTGTCTCTCTGAGACATTTCCTGCCACACAGGGTTAGGGTTT







AGTGGGAATGTTGGGCACTGCCAGGTGCTGTTTTCCTTCCTTGAT







TACAAGGCAAGCCAGGCATGCACCCAACACGGGGCTGCGAGTGGA







TGAATAAAGCGTGCTCAGCGGGCTGCAGAGGGCTAGCCAACAGGG







CCAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene TXLNGY. In some embodiments, the sequences from gene TXLNGY comprise or consist of:











(SEQ ID NO: 679)



AGAAGAAATTGTCCTGGATCATACAATAAGCTAGGATTTGCTCCA







GACCATTTTTTTCATTTTATCAGGCTACCTACAATTAAGGAATTT







CAGAAGATTTTGTGAACAAAAAATGTCCAGGAATTATGTGTGTAA







TAAATGAATTTGATATTTTGATCTCCTTATCCATCTTTTTCTTGA







TAAAATATCAGCTTTTTTTTTTCCAATGTAGGGCAGAAGCAAGAA







GTACAGATATTTTGATGGGGACAGCCCACGTCTGATAGGGTGGGA







CATGGGGCAGCAGTTAGAGGGTTGTGCTCTTTCTAGTGTGGGATA







GTTTGCAAGATGATATGTTGTAGCCACTTCTGCAGTGACGGGGAC







AGAAGGAGTCAGTGTTCTTAACTACAGTGGTAGCAGCAGAAAAGA







AGGGTAAGCAGGGTTCTAGAAATTTGTGTTATGTTTTCTCCCCAC







TGTATTTATTTCTTTGGTTAGTGGTGCAAGAAATTCTGTTTTCCT







GTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene TXLNGY. In some embodiments, the sequences from gene TXLNGY comprise or consist of:











(SEQ ID NO: 680)



TAAGTTCTACTTCTTGGAAAAAAAAAAAAGATCGCCAAAATTGTA







ACCAAAGCCATTTAAAACAAACTCCTCACTTAACATAGTGCCAGG







GCATGCAATTTTGTTTCTTACTCTCTGGATGTGGGATATGCGAGT







GTGTGTGTGTGTGTGTGTGTGTGTGTATTAAGCTTTCTGTTTCTG







ATGAAATTTTCATATGAAATTTACTGGAGATAAGATTAAATTAGT







GGAAAAACAAGATAATTTTGCTTTCTATGTAGTGGTCACCAAGTT







AAAGAATTGTGCCAGTTATTAGGCAAGATAGGCTTTGAGATTTAG







GGACAGTTAAATTTTACACCAACTTAGTGAACATGAGACTTCTAC







CTAGTGTTACATTTCATTTTTAAATAAGCAATTTAAAAATTGGTA







AATGATTTGTTTACTTTGATTATATTCTGGTATAATTTTCTGACA







AAATTATCTGTGTCTTGGTCAGCATTGTTGCTAGAATATGTATTC







AGAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MICU1. In some embodiments, the sequences from gene MICU1 comprise or consist of:











(SEQ ID NO: 681)



GTACTTTTTAAATTGATCTTTGAAGGCTTGGGCTCTTTAATGGTA







GTTTATAAATTATTAAAATTTATTCATTAAAATATTACTCTCACA







TTTCTGGTGGCTGGCAGTCTTAAAAATATAAATTAAACTGAGTTG







TAGTAATTAAATATAAATTAAGCTGAATTATAAACATCTTTTGGT







TTAAAATAACTAATATAACTAACGAAGACCACAGGGTGCTCTTTA







TTTTTACAGTACATTTTTTCTATACTTCCTTTGTTTGAAAAATGG







GGTGTAATTTTTGGTAGTCCCTCTTACCAAACCCTCAAATTAGGT







ATCTTATTATTAGTACAATGGATTATCTTCCTAAATACCTTTGTC







TTTAACTGCATTCTGAACCAAACCAACAAATACATTAAAAATTAT







GTCAGAATAACGTATAGACGATTCCATTATCAATGTTTCTAATCC







TGGGAATTCTATTTTAGAACAGCCATGGCACAAACATCATACAGA







GTAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MICU1. In some embodiments, the sequences from gene MICU1 comprise or consist of:











(SEQ ID NO: 682)



AGGTTAAGAAATTTTGTTGTAATCACTCTTGTTAAGCATAAGAGA







TACTTAATTAATTGAGTCACTTAAATAGTCTTACTTGGAGGCCGG







GTCTGGTGGCTCATGCTTGTAATCCCAGCACTTTGAGAGGCAGAG







GCAGGAGGATCACTTGAGCAACCGGGAGTTCAAGACCAGCCTGAG







CAACATAGCAAGACCTCATCTCTATGAAAACATTTAAAAGCAGTA







GTAGTCCTAGCTACTCAGGAGACTGACGTGGGAAGATCGCTTGAT







CCCAGGAGTTGGAGGTTGTAGTGAGCTAAGATTGTGACACTACTC







CAGCCTGGGTGACAGAGTGAGACCCTGTCTCTAAAACAAAAACAA







AAAAGTCTTATTTGGGATATTATGTTCCTACTTGAACTGTAACAG







GACTGATGAGACAACAGTAACTTCAAGTTAAAAATTAATCTTTTT







TTCTTTTGAAATTTCTTACATAATAAATACAAATAAGCAGTTGCT







TGGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene VDAC2. In some embodiments, the sequences from gene VDAC2 comprise or consist of:











(SEQ ID NO: 683)



GGCGGCCAAGCCGGAGGCCCGCGCCGGACTCGGAGTCGGCCCTGG







CTTCCTAAGATGGCCGCGCTGCCCCGCGGCCCCTCGCCCCTCTCT







GCCCGGGATCTCCCTTTGGCCCTTTGACCCCAGCTTACCGCACTT







CTTGTCCCTCCCGCAGATTCCCCTCTTCCCGCGGCCTCGCCATGG







CGACCCACGGACAGACTTGCGCGCGTCGTAAGTAAAGCTGGGATC







TCTGCGGGATGGGGCGGCGGAGAGTCGAAGCCTGTCGACCAGAAA







CCCAGTTTGTTTCCCCCTATCTTTCCAAGAACTTGGAAATTCGGC







TGCTTTTGGTGGTCGCCCCACCGTCTGACCACCTCCTCTGCGGAG







GAGGGGCTGGGCTGCTGGATTTCAAATGGGGAACAAGGCCCGGGG







AGTTGGTCTGTGGCCGCATGGACTTTTCAGCCTTTGTACATGTCT







TTGACGTATAGAAGTAAAAAGTTGTTAATTGGGGATTCTGCCTTT







GGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene VDAC2. In some embodiments, the sequences from gene VDAC2 comprise or consist of:











(SEQ ID NO: 684)



TAAGTTTGAAGCGCTATTTGATATTTAGTTGATGTAGACATTTGA







GTGAAACACACATGGCAGTATTTTTTCCAAGTTTCAATATTTAAA







TTCCCAGTATTAAATTCTCTTCCCACTCCAGGGTGTTGGTTTTCC







CCTCAGCGAAGATATTAAAGGACGTATCAGGGTAAAGGGCCTAGT







CTTAGGTTTCGGTTTTATCTAATTTAGTTAAATCAGCTCATCTCA







GTGAATTTTGGCAGATACATAAATATCCATACACACAAATACTTG







TTTGATTCTTTGTACTTGTCCAGTCCTAGGAAGTGAGGCTGTAGG







TAATAGACTGTTTTTCCGTTTTGAAACGAATTCCCCAAGTTTGTA







TAATCATGGTTATCTGTTTTAGAAATAATTCGTTGGTGGTATCTC







CTTTATGGGTCGTTATAGTTCTATTGTAGCTCCTGACCGCATAAA







AAACATTCCAACAAATGGGATTGCTTGAATTTTAATATCAGCAGT







GGGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CYP2C8. In some embodiments, the sequences from gene CYP2C8 comprise or consist of:











(SEQ ID NO: 685)



GTAAGTCTGCCTTATGTTCCTCCAGCCAATTGCAAAGGGTAAGTT







ATTTGACTGCTATTTTTAGACAAAATATATTCCTGGAAGCACACT







ATTATAATAGATCATTGTAAAGCAAAATACTCCCTCTGAACTTCT







TTGATGTTTCTTTTGTCTTCCTATTTTTTTTTTTTTTGAGACGGA







GTCTCGCTCTATTGCCCAGGCTGGAGTGCAGTGGCACTATCTCCA







CTCACTGCAAGCTCTGCCTCCCAGGTTCACACCATTCTCCTGCCT







CAGCCTCCCCCGAGTAACTGGGACTACAGGTGCCCTCCACCATGC







CCGGCTAATTTTTTGTATGTTTTAGTAGAGACAGGGTTTCACCGT







GTTAGCCAGAATGGTCTCAGTCTCCTGACCTCATGATCTGCCTGC







CTTGGCCTCCCAAAGTGCTGGGATTACATGTGTGAGCCACCGTAC







CTGGCCTCTTTTGTCTTTCTAAGTCTGTCATTGTCAGAAATAGCG







GAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CYP2C8. In some embodiments, the sequences from gene CYP2C8 comprise or consist of:











(SEQ ID NO: 686)



TATACATTTATTAGCTAATAATTTGATATATGTATGGTAATTCAA






CATGTATGAGTTATATTCACTATTTCATGTTTAGGCAGCTGTATT






TTAAGTGAACTATACTAAATATTTGAAAGGCTTTTGTTATCAAGG






GCTAAGTCTCCTATTTTTTGATATAGCATTACAATGTACATTTTT






TATACACAAAATATAGAATACACTGATTTCCCTCAAGGTCATAAA






TTCCCAACTGGTCATTAATCTGAGAATATTGAATTTTGAGTATAT






TCTAACATAGAATCATTTACTTCAGTGTTTCTCCATCATCACAGC






ACATTGGAACAACCAGGGACTTTTAATTAAAAATACCTGGGCTCC






AATCCAATACAATTAAACCAGAATCTCCTAGATTGGCACTGGAAA






GAAGGAGTAGGACAAAAGAACATTTTATTTCTATCCATGGGCCAA






AGTCCACTCAGAAAAAAAGTATAAATTGGATCTAGGTGATTGTTT






ACTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene GPAM. In some embodiments, the sequences from gene GPAM comprise or consist of:











(SEQ ID NO: 687)



GTGAGTGAAGACCCTTCAGTGTTGCCTGGTCTCTTTTCTTGAGCG






TTTTCATGAAAACGTAACCCCCCGTGTTTTTCTGTTAGATAATGT






TTGACCCATTGTTGATACTTTATATTCCACTCCTGGATTTCAGTG






ACCACCAAACCAATATGTCATTTACTATCTTGGTTTGCTCTCTGG






GTCACTGCTCCACTGAATCTATCCTGCAGCTATGAGCCATGATAG






GACAGATTGCTCAATGTTCTCAGGCAGTCACATCCAAAGCTAGTG






AGCACGTATTGTGTGTGAGGCACTGGGTCAGTGGTTTGTAAATAA






TTAACACACCCACCTGTAGATGATGGAACCACAGATGGTAAGAGT






CAGAAGCAGCCTTAAAAATCATCTGCTCGGCCAGGTGCGGTGGCT






CACGCCTGTAATCTCAGCACTTTGGGAGGCTGAGGCGGGCAGATC






ACGAGGTCAGGAGATCGAGACCATCCTGGCTAACGTGGAGAAACC






CCGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene GPAM. In some embodiments, the sequences from gene GPAM comprise or consist of:











(SEQ ID NO: 688)



GCCTAAAACACATAGTGGTATCTGCTTTAATTTGGGTGATAGCCC






CTTGCTTGAGGGAATGTGGCTATAATTTTAGTTTTCAAACGAAAA






TGTAATCTCCTGTTATATCACTGTTTACAATGAGTAGTTACTGAA






TTACCGTTTGTATAGCATTACCCTTATTTTTCAGGAGAGGTAGAG






TGTCTTGCTATCCAGGAGCTGAAAATCTAACTGAGATGAGACATA






CACAGAGCAGCAAAGTGAGTGGAGGGCCTCATTTGTGATTAAGAG






AAAAGAGAGGAAGGAGAGAAGAGGGGGCTTATGTCTTTGGAGAAG






GTTTTTGATGCAGTTGGGATCATATGGAATCATAAGAATTTAGTC






TTATGGGATTTACTTAAATTTCTCCAAATACAGGCCATTATTTTT






GATGTATTAATAATCTCTTAAGGTTATTCAAGCCAGTTGAAGTTT






TTCTTGGAGACTTTGAGCTGGCAACTTCATTGTTGCTTTTCTTTT






CTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ACSL5. In some embodiments, the sequences from gene ACSL5 comprise or consist of:











(SEQ ID NO: 689)



GCTACCTATTAGCTACATAAAGTATCTTTCTCTCCACCTCTTATT






TCCTACAGGTGACCCCAAAGGAGCCATGATAACCCATCAAAATAT






TGTTTCAAATGCTGCTGCCTTTCTCAAATGTGTGGAGGTCAGTGG






TCAGTTGAAAAAGAGGCTCTCATTAAAATGTGAATCTGTCATAAG






ATTTTTATTTTTGAGGTTAGAGCAGGCAGACACACACACACACAT






ACACACACACACACACACACGTTAAAGGCTGGAGTGTGGACAAAC






ACCAGCGTGTTCTTTGTTTAGAGGACTGTACATTTTCAAGCGTTG






GTCTTTTAGGATCACTGCAAATTTCTACACTATATGCAGTTCAAG






GCATCTCAGATCTACACAGTGTACCGCCTATACAGCTGTCTGTGG






TAGTCCTGGGAGGAGGTAGTTTTAAAATCTCCATTGGAATTGAAA






GTTGGCATTAATCTCATGTTGCCTCCTCTTACTTCCCTGGCCTAC






ATAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ACSL5. In some embodiments, the sequences from gene ACSL5 comprise or consist of:











(SEQ ID NO: 690)



TGAGTGTTCTGTGTTCCTAGCAGTATGTGGCAAGGGGGTCCTGAC






TTGCTATCACATGTTTATTCCAAAGGCAGCTACACAGTATTTACT






GTTCTTTCTCCGGTGTGAGAGGTGCAGGCAAGGAGTTGGCTCATG






GGAGCCCTAATCATGGCATGGCCCCTCATTTCCTGCAGAGTCTTC






CTCTGCTAAGTCTGCATGTGTCCAGGGAGGATAGTACTTGCTCCT






GCCTTCATGGGGGTGTGTGAGTCTAGTGACATCACAGCTAGGAGC






TTACTATCTATCTGCCCTTAATTCTGAGCTGACACAAGACATTGT






CTTCTTTTTAAATTTTATTTTGGTTAGAATTTTTGAAAAGATCTA






GGATTAGAGAAAAGGAAGTTCCCTACAGAGGATCAGGGAAGGAGC






ATTTGATCTACATAAGGTGTGAGATGTCCCCCGCGCCGGTTTTTG






CACACCTGAGTGTGTAAGGTGGTGCTGTCAGCATGGTGCATACCG






GAGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene HABP2. In some embodiments, the sequences from gene HABP2 comprise or consist of:











(SEQ ID NO: 691)



TGAGGTTTAAAACAAAACTTTTGAAGCCAACAAGCAACTGTGGGC






AACACTTCAAAGGGCCCAGAATCAATGTTTGTCAATAGCAGTTTT






GCATCCTTTAATTTATGTCTCATGAATATAAAGTGTGATGTTATT






AGGAGCCCATCCATGCCTTAATTCTGTTTGAGATTTGATGAACCA






GAAGTCTATTAACATTCTGTACAAATCAGCACTGGCAGAGGCCTG






TGGCACAGGCTTCACCTGATCTTTTATCCTGAGGCTGGGATGCTA






GTCACGCTGTCAGGCTTCCTGCTCGGAACACCCAGCAGCTGTCAT






GTGCACCGTGGAGCCAGGGGGCTATGTCAGCTACTTTCTTTGGAG






AGTTTCTCCCCAGAAGAGAAACCACTTCATGACTCAGCCCAGCCC






CAAAGTTGCCATTTGTTTAATCAAGATAGTTAGTTTGCAAAACAT






TAAACATTAAACAGTATTTGATGCCTAGTTAATAATTTGCATCCT






CTTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene HABP2. In some embodiments, the sequences from gene HABP2 comprise or consist of:











(SEQ ID NO: 692)



TAAGTGTTCTTTTCTAATATTTGTAGTTGAGAGACTCCGAAGTTT






ACTAGGAGGACCAAGCTAGGCTGGGAGTGATGAGAAACTTTAGAG






TGAAAGGCTGGAGTTGCCAAGTTGGAGATGTTCTAACTACAAGAA






CCAATGCAGGCCTGTGGCTTGGTTTCTTTTGAGCAGCCACTCCCA






TGGGAACAGGTGTCTGACTTGAGCCATCTTGGAGCGGAGCCTCCA






TGAATGCATTCCAGCAAAGGAGACTGGGGCAAGAAAATGTGCCCC






AGGGCATGGATTGTAATCTGTCATGGATCTCATAGACCCCTTTGA






GAAGCCATGGAAAGCTATAAACTCTCTCCCCAGGAAAATATACCT






ATCTATTGGTGCTGACACACACAGTTTTCCAGCGATTTGTGGCTG






ACACTAGGGTAAGAAGCAATGTGCTATGGCCTTAGAGGGGAAGCC






TGCTGACTGCAAAAGATCCTGGCAGTGGGTCTGCATAAATAGTTT






GGAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene IGF2. In some embodiments, the sequences from gene IGF2 comprise or consist of:











(SEQ ID NO: 693)



GTAGGATTTGAGCTGTGTTTCCCGCCCTGATCCTCTCTCCTCTGG






CGGCCGGAGCCTCCGTAGGCTCCAAGCCTGGCCCAGATTCGGCCC






GGCGCAGCCGGCCTTCCGCGCGTCCCGCACCTGGCGGGGGCTCCG






GGGCTCCGGCGCGGCACCGGGGGGCGCTCGGGATCTGGCTGAGGC






TCCAAGCGCCGCGTGGCCGGCTCCTCCTGCTGGGGCAGGTGGCGG






CTGCGCGCCCCGCCCGAGCCCAGGGGCCCCCTCAGCCGCAACAAC






CAGCAAGGACCCCCCGACTCAGCCCCAAGCCACCTGCATCTGCAC






TCAGACGGGGCGCACCCGCAGTGCAGCCTCCTGGTGGGGCGCTGG






GAGCCCGCCTGCCCCTGCCTGCCCGGAGACCCCAGCTCACGAGCA






CAGGCCGCCCGGGCACCCCAGAAACCCGGGATGGGGCCCCTGAAT






TCTCTAGAACGGGCATTCAGCATGGCCTTGGCGCTCTGCGGCTCC






CTGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene IGF2. In some embodiments, the sequences from gene IGF2 comprise or consist of:











(SEQ ID NO: 694)



CCGCATCTGCTGCTGTCCCGCCGGTGCTGGCGCTCGTCTCCGGCT






GCCGCCGGGGAGGCCGGCGTGGGGCGCGGGACACGGCTGCGGACT






TGCGGCTGGCGGCTGCGCTCGCTCCTGCTGGGCGCCCCGAAATCC






GCGCCACTTTCGTTTGCTCATTGCAAAGATCTCATTTGTGGGGAA






AGCGGCTGGAGGGTCCCAAAGTGGGGCGGGCAGGGGGCTGGGGCG






AGGGACGCGGAGGAGAGGCGCTCCCGCCGGGCGGTAAAGTGCCTC






TAGCCCGCGGGCCTAGGACTCCGCCGGGAGGCGCGCGCGGAGCGC






GGGCGAAGTGATTGATGGCGGAGCGAGGGGGGCGAGGGGGGCCAG






GGGGGCGCGAGATTCCGCCGGCGGCCCCTTCCCCTTGGCTAGGCT






TAGGCGGCGGGGGGCTGGCGGGGTGCGGGATTTTGTGCGTGGTTT






TTGACTTGGTAAAAATCACAGTGCTTTCTTACATCGTTCAAACTC






TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PSMA1. In some embodiments, the sequences from gene PSMA1 comprise or consist of:











(SEQ ID NO: 695)



GTGAGAATATCTTTGCTTTTTGATAAAACACAGAATGCATAGATT






TCACATCTCACTATGTGAGCCCCCACAAAACAAAACCTAGAGGAG






CATGTGAGAAAAACGCATCTCTAGGCTAGAAAACAAAAAGAGAAA






AAAAGAAAAATGTCTGTGTAAGAAAGCAAAGGAAAGAAGATGCTC






AAATCCAGCAAATCATACCAGCCTTGTCATAGGCACAAAAAGAAA






GGGATTAACCTAAGTTACAAGGTGAATTATCATAAAATCAGATGA






ACCTTTCTGAAAAATAGTCATGGGACAAAGGAGGGATATATAACA






CTAGAGGCTTAACCCTTTCCTGCAGAGAAAAAAAATGTAACTGAA






AGAAATGCCACTCAAGGCTTGTGACATTCAAAGTGAAGACAAGAT






AAGGTCTTATGTAGTACATGACATAGGTGTAGACACTCACTAGAC






TGAAAGGAGTACATGTTTCCAAAGCAGCTTTAATTCACGTCTCTG






CTGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PSMA1. In some embodiments, the sequences from gene PSMA1 comprise or consist of:











(SEQ ID NO: 696)



TGACCTAATTGTATTCATTTGTCAAGAGGCCGGTTCCATATCGGA






GACAGGCTGTATTCATAGATGATTTTAAGGACAGTTTAAGGCATA






GAGTTTTACTCTAAAAACTGAGAAACAAAAAATCCTTCATTGTAG






AATTTGTTTTGTTGCCCTAGCTCTACTATTTATACTTTTCCACTT






TAGCTATGCACAGAACATAGCTAATTTTCATCTCAATTTTTCAAT






TCTTTTGCTATATAATTGTTAACATTTATTGACCACTAACCTCAT






TTTAAGGACTGTGCTAAGTATTTTATATACTTTCCTGGGGAGGCA






CTTATTCCTCACTTTTGATATTTCCCTCCACGTGGGGAACATCCA






TTAGAGTAACTCTCCTGGCCTGTATCTACTTACCCCCAGGAGAGG






AGAAGAGAGTTTGGTGAGTTTGAGATGAGGAACAGGATGTCTGAT






GAAGATCTAATATAACTCATGTTCCTTTTACCTTCTCCATTGTCT






TTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene DEPDC7. In some embodiments, the sequences from gene DEPDC7 comprise or consist of:











(SEQ ID NO: 697)



TGCCTTTATGGTATTCACCATTCATTGTTAATTCCTAGTCTTGCT






TTTGCTTTTGGAAATAATTTTGAATAAGTGCACTTTTTAACTCAA






ACTGTAACTTGAGTTTTGTTCCCTTTAATATAGTCATATTTGGTC






AATTAAAAGCAGTTTTTTTCTTTTGGCTAAAGTGAATGGGAAGAC






GGAAATAGCTTTAGAAGCTACCCAGCTCCTTCTAAAGCTTTTAGA






TTTCCAAAATAGAGAAGAATTTAGAAGACTACTGTATTTCATGGC






TGTTGCAGCAAATCCTTCTGAGTTTAAATTACAGAAAGAAGTAAG






TCTTTTGTTTAACTGTTAGTTGTATTTAATATCCTTAATACTTAC






AGGTTAAGTATTAGATTATTCCATCTTCAGCATCAAGTATGCTGA






AATGTATAATAGCTATTAATATTTAATATTCCCTTTTATATTAAA






CTTGTCTCTTAAATATATACTATTGGATATATTTGTTTTATTTTT






ATAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene DEPDC7. In some embodiments, the sequences from gene DEPDC7 comprise or consist of:











(SEQ ID NO: 698)



TAAAATTGTGAAAGTAGTTAAATTGAGCTTATGTAATAGATCACT






AGATTAAAATGCATTTGAATAATGTCCCATGTCCCTTCTTTTTCT






TAAGATTCCTGGAACTCTACATAAAATTGTAAGTGTTAAGCTTAT






GGCCATACAGAACGGAAGAGATCCAAATAGAGATGCAGGTAATCT






GAGAAATATTATTTTCATGATCTTCCAAAGACAAATTTCAGGTGT






TTTTTGAAAATGTTTTGCTATAAGTAGTTGGACTTTAATTTCAAC






CAAATTTAAAACATTCTCCTCAGAGTTATTCATACAAAGCAACTT






TACAAGTGTTAGAAAGTCAGCAGTGCTTACCATCTTCAGTGCATA






TTACAAAATGGGGAAGAAAAACATAAAGACAAGAATATTGCTTGA






TTTTTCTGCTTAAATATGAGGAATTGAGTCATTAACTGAACTTTT






TACTTTTAAACTTTAAGGATATATTTATTGCCAGAGAATTGATCA






ACGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CTNND1. In some embodiments, the sequences from gene CTNND1 comprise or consist of:











(SEQ ID NO: 699)



TGAGAGAGGAGACCACAATAGAACACTGGATCGATCGGGGGATCT






AGGCGACATGGAGCCATTGAAGGGAACAACACCCTTGATGGTAAA






TTCTCTTTATATACTGCTATCTGTCTAAGGCTAGTTCTATTTTGA






AGCCTATGTGTTTTGTGAAACACAAAAAAAAGTACAGAGAAAGAT






CGCATCCTTTTCTGGTAGGGGTTTTCAGGAAAAAGTAAGAGTTCT






GACTCATGTTGGGATTTCTTGGGCCGTTATTCTGCAGTGGTCAAA






ATGGGGGAAGCATGTCTGTAAAAGTGTTACTGATATGACTAACAC






TAACTGATCTACTTTCAAACATTACCTTTTTCCTCTCCCTCCCTG






TTTATGAATGTTTTGCCCTTCTCTTACTATTATATCTTGTTTTGT






TTGAGTTTACAAATTTTCAGAGTTTCCCTAGCTCTGGCACACACT






CATGAGGTTCCTGTCTCTACTCATACTAACAAATTGCATGTGTTC






ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CTNND1. In some embodiments, the sequences from gene CTNND1 comprise or consist of:











(SEQ ID NO: 700)



TATGTCCTTCAGTCACCCCCAAGATTGTTCTTGAGGAAGGAGGCT






CCTGAGTGCCAGCAACTTGTCAATCACGCTAATCTGATCAGGCCA






GAGACTAAGTAGTCTCAGCCACATGGGGCTCGAGGGGTTCTGCTT






TTGTGATTATTACCTCTTACGAGTCTGGGACATGCAGCTGGTGAT






CTGATTCTCCGTTATGTGCTGCTTTCCTTTCTCCAGATTTGCTCA






ACTTTTTTGGGGGGGGTCTCCTTAATCCCAACCCCATTAACATTC






TCTCTTTCTCTTTTTTCTCCACAGCAGAAGATTTAGCACCACTAT






CTCCGTTCCATCTGGGCTTATATGTACTTTTATTTTTTGGTGGTG






AAATTGACTGATGATTTTCCTTTTTCTTCGCTGGACTATTGTGCC






AACTGCCAGGCTGCCTCCTGCCCTTACAGCCCTAAGTGGCTGCCT






TCTTTCCATCAACTCCCAACTTCTTCCTGTGAAGTTTAATTGTCT






CAAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MS4A6A. In some embodiments, the sequences from gene MS4A6A comprise or consist of:









(SEQ ID NO: 701)


GTGAGCAAGGTTGGAGGTGATTCTGCCCCTCTCCCCAGGCTTTCTGTGT





GAGTCCATTCCTCCTCTCAGATTTATTGTTAGAACTTAAGACAAGCCAA





TTACATTTCATAATGTCTGTGTCATTCAGACGCTGAGAACTAATCCAGC





CTTCTAAATGGTGCTTCTTAACTCCTTTCTTCAACAGGAGAGTTAATGT





GTGGAATTAAGTGCAGAATCCATGGCCCTCTGTGGTGACGGTGATGGTT





CAGGTTGTGTTTCTGGGTTCATTCTGGAAGCTCCCCCAAGGAAAGGAGG





AAGGAAGCTTGCAGGGTGGGGCTTTGCCATTGCCCTGACTGGCTCTGGT





TTCCTTGCCTGATACATTGAAGTCAGCTTCCAAAGAATGCCACCCAAGG





TTTTGAAGGGGCACAGTGCCTCTGTCCTCACAAAACCAGCCTGCATATG





AGTTTACTGAATTCATTTAGGTCCTGCTGAATCTTTCCCATTTGTTCCT





TCTCTGTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MS4A6A. In some embodiments, the sequences from gene MS4A6A comprise or consist of:









(SEQ ID NO: 702)


TTCTGGTTGTTACACATAGGGTGGGATTGTGCTGGCATAGGCCAGGGAG





TCTGCTAAGCATCCTACAGTGCACAGGATAGCCACCTACTGTAAAGAAT





TACCCAGTCCAAAGTGGCATTAGTAGCTTTTTTGAGAAACCTGTCCCAG





ATCATTAATCAGAACGTGTTATTGATATACCAGTCTTCTCCCTTGTAGT





TAGGTTGGTAGAATTCAGACTCTGGAAAAAACTGGGAATGTAAAAGGCT





GAATGAGTGTAAGCTGAAAGACAAGAGAAAGGAGAATTGTTTTAAGCTT





ACTAAAGCCAAAATGGAAAAAAAAAGAGGGGGGAGGATTGGGGGGTGAG





GAAGACAGTCAACAGACCGCTTTTAGGCCATCATTCCGAAGGGTTCTGT





GCTAGGAACCTTACACGTGTTGTCTCACATAATCCTTAAAACTCAGAAA





TGATTTTAAATTAAATAGCACAGAGAGGAAAACTGACTGGAGAGGGAGC





CTTTGCTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SLCO2B1. In some embodiments, the sequences from gene SLCO2B1 comprise or consist of:









(SEQ ID NO: 703)


AAAATGGGGCTAATGTACACCTCGCTCATAGGAGTGATGTGGGGTTGCA





GGGCAGAGTTACTGAGCCCGTAGTAAGCGCTCCATAAATGCCAGCCACT





GGTGTTGTCTGGCAGGAGCTGGCGTTACAGTGGTTGGGGATGCTGGGGA





AGTGTCTGACTGTCCCAGCTCCAGGTCTTGCCAACTGTAGGAGTAAGGG





GAGAATTTGATGGGACCAGTGGGTGGAGGAAGAGGAGTGATGTCTGTGA





CCAGAAACATACAGAGGTCAGCACAGGACCCTGATGGTCAGTGGGTGTG





TTGTCTTGTATATGGGACCAGGCTCTGCAGGTTGAAAAAGGATGGGGTA





CGTCCCTGAGCCCCCAGCCTAGATCATCAGATGGGAGAGAGGCTGGCTC





TGGGTGGTGGGAGAGAAGGGCATAATAAGAACCCTTCAACGTTGATGCA





TGGCCCTTGGGGGCTGGAACCCCTCCAACCTCATGTTGCCCATGTCTCT





GCTTGTTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SLCO2B1. In some embodiments, the sequences from gene SLCO2B1 comprise or consist of:









(SEQ ID NO: 704)


TAAAGATCTTGCTTGGGACCATTGGTGGTGGTGATGGGGTCCAGATGCC





CACTGTGTGCCAGGCCTGGGGCACAGGCATGAGCGGAATTCAATCCCTG





ATCTTAGGGCTCACAAGCTCATGGGGTGACAGACCTGGCCCAGCTCTCC





TAGAGCGGGGTATAGAGGCAGAGTCTAGACAGCCCGTCAGGAAGCCACC





TCGGATGCAGGGGTGGGGCGGGCTTGAGGGCCAGACAGCCAAGAGGCCC





CAGTAACCCAGAGCCCCTGAGGGGTTGTACATGCCAGGGAGGGGGCAGG





GTAGGACGGTGGGCCTTCATTGTCCCCTGAGCACCACCTCCCTCAGCCT





GGATGCCCAGCCCCGTGATCCACGGCAGCGCCATCGACACCACCTGTGT





GCACTGGGCCCTGAGCTGTGGGCGTCGAGCTGTCTGTCGCTACTACAAT





AATGACCTGCTCCGAAACCGGTGAGACCTGGTTTGATGGCTTGTGGGAA





GGGTGCTGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PICALM. In some embodiments, the sequences from gene PICALM comprise or consist of:









(SEQ ID NO: 705)


GTAAGTAAATAAGGAGAGCTTCAATAGTTCCATGGTAGAGAGAGGCCTG





CTGGAAGGTTCAGAAAATAGGCCGTTTGGAACCTTCTCCTTTAAGCCAG





GAGGGCGGCTTTGGGAGGCGTCTTGAAATACATTCTTGACTCTGCTGGG





AAAGCTCGGGGAGCCAGAGGCCTAATACCCCCCACTTCAGGGGAAGCTG





TGAAGGCAGCTGCACCTCCTTTGTCAAGCTGTTGGTTTCAACCACAGAC





ACGAGGACACACTTGAGGTGGGGGGATAAGGGGAGAAGCCTGCCGCCCC





GCCCCACCCCCGCCAGCACACCGCCCTCGCCATTCCTCTCCCGCCCCGC





CCCGCCCGGCGTCTGTGACCCCGCGGGCCGAGCCTCAGGCGACCTGTTG





GCAGGGAGAGTCACGTGATGCGCCGGCTGTGCACTCCCCTCGGGCGGGC





CTCCTGTTGCGCCCGCCTGGCGAGTTCCATCACGTAACGCGCGGGCGCC





GCCCAGCTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PICALM. In some embodiments, the sequences from gene PICALM comprise or consist of:









(SEQ ID NO: 706)





TATTTTGGATTTGGCTGGCAGATGGTGTACACAGACTTTCTGAGAATGT





TAGCATTTCATTTGAAACTAGCAAGATTACATGTGACTATCATGGTAAC





TTAGCACTCACATATTTTTCAACTTATCCCTAACCCTGGGTTGAATGTA





GAGGCTCTAATAACATATAAAAGGCTTTGTTAATGTATGGTCTGGGTCT





CATTGAATAGTATTTCAGGATGTAATACTTCTTTTAAAATGTCCACAGG





GCAGTGTCAACGTCTTTCCATGGAAATCTGGCTCACCCAATAAGAGAAA





AGAGGCCAAAGGTAAGAAAGACTAAACCAGGGGAGATGGCAGGCAGTGA





TGTCTGGACTGTTTGAATGGGACTTCTCCATTTACCGCCCCTCTCACTT





CCTTCTATGGTCTGGACCTTTAATCACCACAGCAGTCAGCCATGTAGGG





TCAAGCTAATCGCTGTTGACATCATCCTACTACTTCCCTTTTCAAATTC





CAGTGAAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SLCO1B3. In some embodiments, the sequences from gene SLCO1B3 comprise or consist of:









(SEQ ID NO: 707)


TTTAGTATGTGACTTGCAAATTTTTCTTGACAGCCAGACAAAATGTATT





GGGTAAAAGGAATTGCTGTATATAAGCCTTTATTAATGTGGTGTAAGGC





GATGAAGACAAAGTAGCATCCTGTAGTTCTATGATAAGGCCTCAATCTT





TTAGTGAGCCTGTACCTCTGGACTGTGAACTTCAAACTTGCTTCTCAGT





TTCTGTCTCCCACTCCTTAGGTAGAATGAGATGGCTTGAGGGGGCTGGA





GTTATGTATTTTCCTCCCTCCCAAAGGAAGGCTAGAGACAGCTGGGGTT





TAGTTTCTGTAGCTTCATATGTTTTTTTAATAATAGAAAAATATCTTAT





GCCCCCAATGAAAATAATAAATAGAATTGTTCTCTTAATATTTGTATGT





GTAACTTGTTTTTCTTTCTTTTAAGATATGCATACTGGGGAGAAAAAAA





TGTAAGATATTTTACACATTTAAAATAATAATCGACTCTCTATTTTCTC





TTTTCACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SLCO1B3. In some embodiments, the sequences from gene SLCO1B3 comprise or consist of:









(SEQ ID NO: 708)


ACCCATTGATAAGATGTGTCTTCATTGCATCTGTCTGTTCTCTGGGAGA





AGCAAAATCTGTGTTACTTCCTTGCATACTTGTTTGTTTATTCATTGTG





CACAAGAAGATATCTTCCACATCTTCACAAGCTGGATAAGAAATAGAGA





AAATGAGATCAAATATTTAAATATTGCATTTTCTGGTAGTGATGCAGGA





TATTTCTTGGCCTCTTCACTGGACTCACAATAGAGGTGCCCTGTTTATT





TGGTCTGCCATGCTCAACCCCTTGTGGGAGGGAGAGTGTGAGTGACGTA





GTGCAGGACCATGTGGGCTGTCCTGGTCATCGACACAGAAACAAGCTCT





GTGAGTTTCTCATGGCCAGACCAGGTGCGAGTGAGTGAATGCTGGACCT





GGGCAACCGCTCCAGGCACTGAAACGAGTGAGTTACATGCAGGGCTGGC





AGCCAGGCAAGGTGCAAGGGTGCTCAGGATCCTGAAGCCCCAGAGGGGT





TGTTATGGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ANO6. In some embodiments, the sequences from gene ANO6 comprise or consist of:









(SEQ ID NO: 709)


AATTACTGATGCCTGAGGCCACCTCCAGAGATCTGGTTTAATTATTGGA





ATGCAGCCTCCACTGGGATTTCTAAAAGCTCCCAGAAGATGTTATTGTG





CACCAGGGGTAGTGAACCACAGACCTGAGATGTCAGGGTCCTGAGAGCT





CATCTCATCCAGTGGTTCTCAGCCCTGGCTGCAGATTAGCATCATCTGG





AGAGCTCATGAAAGAAGCACTGAGGTCCCACCACCTGAAACGACCTTTC





AGAGGAAGGAGCCATTCTTTTTAGAAGGTGCTCAGGTGATTTTGATGCA





CAGCAAGAGTTAAGGTTAACTGACATCTTAGCCATCCCATGTTATAGAT





AAGGAAAATGAGAGCAAAAATGGTCAAATGGCTTGTACGAGGTCAGGTC





CTTGTTGTTACAGAGGCAGGTCTGATGCCGCCTCCTGCTCTGTCTGCAG





CCTAACACCATCCCTTATATTGGCCTGTTGTATTTATTGATGCCTCCTC





ATCCTTCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ANO6. In some embodiments, the sequences from gene ANO6 comprise or consist of:









(SEQ ID NO: 710)


TAAGATCCCCTTCCTTTGTATTTGAACAGCATCCTTCCTTCTTTTTCCC





CTCCGACTGTCAGTTCCGGGAGAACAGGCCTGTCTTTCCTGTTATTTCT





GTAACAGTAGTGGGGTTTCAGATAATATTTGTTGAATTGAAGTTCTGTA





ACTTTTTATTCCTCAGTCCACATGGATTGCTGAAAATAATCCATTCCTA





TATGCATGCTTCAGAAAAGAACTTCAGAAAGCAAAAGTTATTTTTTTTT





TGAGACAGTCTCACTCCGTCACCCATGCTGGAGTGCAGTGGTGCAATCT





CAGCTCACTGCAACCTCTGCTTCCCAAGTAGCTGGAATTACAGGTGCAT





GCCACCACACCCAGCTAATTTTTGTATTTTTAGTAGAGACAGGGTTTTG





CTATGTTGACCTGGCTGGTCTTGAACTCCTGACCTCAAGTGATCCACCC





ACCTCGGCCTCCCAAAGTGTGGGGATTACAGGTGTGGGCCACCATGCCC





GGCTGCAAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene KRT8. In some embodiments, the sequences from gene KRT8 comprise or consist of:









(SEQ ID NO: 711)


GTGGGTGTGGGTACCTCTGACCGGACCTGCTTCCCTATCCCTGGGACCT





GGGGTGGGGACGGTGGGAGCCCCCTGAAGCCCCTTGGGACTTGGGGTCC





TGTTGTTCTGGGCCAAGAAGGGCTAGGAGTTGGTCCTGACACCCCATTT





GTCTGGGTACAGGCTGGAGTCTGGGATGCAGAACATGAGTATTCATACG





AAGACCACCAGCGGCTATGCAGGTGGGTGTCCCAGGGCCCTGGGATGAG





GGCGGGAGGCAGGGCCCAGGGAGGCCTCAGCTCCAGGGAGGGGGCTGTG





CTCAGTCGCTCACAGTGACCTCAGCCTGAGCACTCATGTTCTTGGGAGA





ATCCTAGGGTGGGGAGGCACATATTCAGGACCCCAGTAATAACTTTATT





ACTTAGTAACTTTACTATTAGAAAGCACCCAAATAAACCATAGCTGTGT





GCCAGGCACTGTGCTAAGTATCCTACAGGTTTTATGTGATTTATTTTAT





TTATTAATT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene KRT8. In some embodiments, the sequences from gene KRT8 comprise or consist of:











(SEQ ID NO: 712)



AAGGGTGTGGAGGGGAAGCACCTGGAAACTGCTCATGTCTCCCTG







ATCTGCTTCCTTAGTCTCGTTTATTTATTTATTTATTTTTGAGAC







AGAGTCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATCT







CGGCTCACTGCAAGCTCCGCCTCCTGGGTTCACACTATTCTCCTG







ACTCAGCCTCCTGAGTAGCTGGGACTACAGGCGCCCGCCACCAGG







CTGGCTAATTTTTTTTGTATTTTTACTAGAGACGGGGTTTCACTG







TGTTAGCCAGGATGGCCTCGATCTCCTGACCTTGTGATCTGCCCG







CCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGTG







CCCGGCCCTTAGTCTCATTTAATTTGAGCTGGGGGGAGTCAGCCT







AGGTGGTGGTGGGGAGGACCCTGAGGGAGGGTGGGAGCACGGGAG







AAGAGAAGGCATACCCAACCTGACCTACTTACCTGTCCCCTACCC







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CSAD. In some embodiments, the sequences from gene CSAD comprise or consist of:











(SEQ ID NO: 713)



GTGCATACAGTCCCCTTCTTCCCAAATCCCGCCCTTCAAACCATT







GGTCCAGAGAATCATCATGGACTAGGGAGTTTAACATGGCTGGGG







TAGGAGGCTGAGGGGAGGGGGACTGGAGAGAGGATTGATTGGAGC







TGGGAGGTCAGACATGGCAGTTCTTCCTGATGCTGGGGTGAGGGT







GAGACCAAAGCCCCTTTGCTGGGGACCTGGAAAAGGAGGAAGGGC







TCTGGTTGGGCAGCTGGAGGAGGAGCCACTGACCTGCCTTTGCTC







CACCCCATCCAGGGCTGACTCTGTGGCCTGGAATCCCCACAAGCT







CCTCGCAGCAGGCCTGCAATGCTCTGCACTTCTTCTCCAGGATAC







CTCGGTGGGTCTGCCCCTGCCCCTGCACCAGCCCCACCTCTCTCT







GGAGCTCTGCCTTCTCTATCCCACCTTAGATAAAGTTCAGGCCTT







TCTCTATTCTCTTTACCATCCCTTCTGAAGCTGCATCTCAGCTAC







CACC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CSAD. In some embodiments, the sequences from gene CSAD comprise or consist of:











(SEQ ID NO: 714)



CACTATGCCTGACTCAATTTTTTTTCAATAGCATTGGATCTTCCA







GTAAAACATTCAAGTTCTTTGAGAGTATGAGCTTTGGAAATGAAA







ACCTAGGTTCGAAGTCCATCTCTGCAGCTTCTGCTTGGATCTTTG







AATCTCAGTGTCCCTGGCTGCACATTAAAAAGGACAGTAACACAT







TGTTGTGAGGTTGAAATGGGCCATTTTAGGCATGCAAAGAGCTTA







GAGCTGGGCCCAGCATCAGGCTCTGAGTAGCTTTTGAGATGACTT







TACATCTTTCTCCCTCTACATTTTGGCAACAGGGTGCTGTGCCGT







TCCTGGTCAGTGCCACCTCTGGCACCACTGTGCTAGGGGCCTTTG







ACCCCCTGGAGGCAATTGCTGATGTGTGCCAGCGTCATGGGCTAT







GGCTGCATGTGGATGTGAGTGGGACTTCGGCTTGGGGGTGCGGTG







GGGTGGGCTGAGCCAAGGCCAAGGCCCACGTTGTTCCTCCTGCTC







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene RDH16. In some embodiments, the sequences from gene RDH16 comprise or consist of:











(SEQ ID NO: 715)



GTAACCACCCAGGTTTGTCAAGTCTGTCTGTGTCTTCCCTCTGTG







TGAAGGGACCACCTCCTGTCTGCTCCCTGGGAAGATTCCTACAGT







GTCTTTCCAGGTTGAAGTAACAGTAGCTTTCTAATATTTCTCACT







TAACAGGCTGCCTGAACCCCATCTCTTTTCAATCCTTAACCTCTC







CCGACATTGGAGGTGTCTAGTCTTGGGGAGAGTTTGCCCTGATGA







GATTTTCTCTCCAGAATTCCTCTCTATCCTCTCCCTCCTGTCTAA







GCAGAAGTCATGTGTTGTTTGATGCTAAGAGCCTGGGTTAGAGAT







CTGGAGACTTGCTGGAGCATCGTTGAGAAGGGAGCACAGGGCTGC







AAGTGTGCACGGAGGCTGTGCCTGTGCTGGAGCCAGGCTTATGTT







CCCAGCACTTTACCTTGTTTTTGCTAGGGCCCATCAGCGTCCACT







GAGAGCTACGATCATCTCCATCTTCAGAGGAGAGTATCAGCTTAA







GGAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene RDH16. In some embodiments, the sequences from gene RDH16 comprise or consist of:











(SEQ ID NO: 716)



TTCTCATCCTAGACAGCCTGACCGTGCCCTAGTTTTCAGAGGAGT







GATTGATTCTGGGGTGCGGAGCAGGATGAGGAAACTCAGGCTCAG







AGAGGGATTTCCACCTAATGTGCCTGGAAACCATGGGTAGCTACC







CAATAAGGCCTTTCTATGTTCAGAAGGTCATGGACCAGGAGTGTT







GGTATCGGAGCTTTGTAGAGACAGTTTTGACCCCTGTACTTAGAG







CAGAGGGGTGTGTTTGCCATGGAGAGTGTGTGTGGGCAGCTGTGT







GTAAGCCTGTGTGAGTTCTGTGTATGTTCCTGTGTGAATATATGT







GTGTGCTCCTTCATGGACACAGCATGCATGTAGTGGGCATAGCAA







GGCAGAGAGAAATCAGGAGACTAGGGGCACATTGCCCAGTAGGAA







GATAGTATCTTCCTACTGGGCAGGACTTGAGGTGTGCAAAGGTGT







GCATGAGTACTGCAAAAGCAGTTCAAAGAGCAGAGAGGTGGGGCC







AGAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MON2. In some embodiments, the sequences from gene MON2 comprise or consist of:











(SEQ ID NO: 717)



AAGCAGTCTACCTTACTTTTTTTTTTTTACTATATTGTTCTGAAG







TCAGGTTATTGCAAGAATAATCCATATTTCATATATTTTTAATGT







AGAAGATAATTGACTTATTTGGGGACAGGTGGAAAAGGGCTTTAT







CATCTATTCGTACTGATTTACTTTGCAGATACAAACTGTATAAAT







AACTTTGAATCATTTTTACCATTCTTTCTCATTTGTTTGCCCTTT







CTGTTGTTCATTCCTGTCCAGTCCATTCTTTACATGATAGCCGGA







AATGATTTTTCCATAGTAGGGCTTTGATCATTTATTTCCTCATCC







TCCCAAAGCAAAAAAATATTTTTCATTTACCGTTTCCTACCAAAT







TGCAAAGAATACTCCAGTCTGACTCCATTCTAGTCACACTTTATT







TCCCACAATAGATTTCATTTCCCCTACTCTGCACTAAACTAAAAT







TCTCAATCTTCCCAAATTTTTTCAACCTTTTGTTTATTTCATTAT







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MON2. In some embodiments, the sequences from gene MON2 comprise or consist of:











(SEQ ID NO: 718)



TAAATAATTTTTCTTTTCATTGAATGCAAAGCTTTTTTATGTGTA







CTTGTCTCGAAGCACTCTATTTGTATTACAATTATGCATGTACAT







GACTCATCTCCCTTTACATTGTTAAGATTTTTGAGGGGCTTTTCA







GACCATGTTTTGTTCTATTTTGAATCCTCTACAACATTGCTTTTC







AAAGTATGGTTTGTTGGACCAGCCATTGATTGGCTTCAACAGTTT







TGTTACTGGTCTGGGACAAGATAGTGAGCTGGCATCAGAATATAA







ATCAGATGTGTCACTAAACACACTGCTTGGTTCAGCTGACTTTTT







TTTATACAAGAAGACATTGGTAAAGAAGAAAACTGTGTGTTGGTT







TACATTCTGGCACAAGCTTCTTATTTTGTAGCAGATTGGCACCTT







AAGGAGCATTACTTTTAATATCCAAGAAAAAAGTAGATTCCTAGT







AAATTACTAATGAATGAGTTGTAGGATATTTGTTCCTGTCAACTA







TTAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene BTG1. In some embodiments, the sequences from gene BTG1 comprise or consist of:











(SEQ ID NO: 719)



CTTCTGACTGTTATTTATGAGCCTTTTCATGGTATTTATCATTTT







AGTATTGAAGATGCAGTGTTCTTTTGGGAATTACGTATGCTTAGA







AGAGAGTACTGATGCTCAAAGGTGAAATGATTTGAATCTAGAGTT







GGATAAAAAGGGAAAGGATGGGGAAAATGAAAACTGGGAAGCTAA







ACTGAGAAGGAATTGCAAGGTTTGGTGATAAAAATTCATTTGCTA







TGGTCATAGTTGTGTATCGAGAGGTTTGCACAGCACAGTCTCTTG







GCAGAGTTTAGGAATCTCTACTCTGGGGATTCTGCAGTTCATGAA







TCGTCAGGGAGACTCTGCATTCAATTTGTTTCTGTGAACATGCAG







GTGAAAGTGGTAGTACAGGTGGAACTTCATGGGAACTGGTTTTGG







GGGAGGTGTGGATGGAAAGCACAAATGTCTCAGTATTTTCTAAAG







GGAGACTATTTTTAAAGGTGTCTTGTTACAAACCACTTGATTATA







GCCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene BTG1. In some embodiments, the sequences from gene BTG1 comprise or consist of:











(SEQ ID NO: 720)



CACACCATTCTCGAGAGTTAGAATTCGTAGACCCGCAACACATGG







ACTACCCTTGCCAATTGTTATGGTTTTATTTTTAAACCTATTCTG







TGAAAAAGCTCCTCGGTATTTCAAACTTGAATTACAATAAAAACA







CCAGGTAGGGTTGTTTTTAAATGGGTCTCACCCCAGTGCTGACTA







CTGTTGTTTACCAACTACATTTCATCTGGTAACTCCATTGAAACT







AAGTACCTCAACCATTTTTTTTTTTTATCATAAAGCCTTTTTTCA







AATGGAAAGTTGTTTTTGAAGATCTTGACATCAGACTTTTTCAAT







CTTTGAAAATGATGGAAACAGTTAATAAAAATGTTTATTTTAGAA







TTCAGGTGATGTCTGGCTTGTGAGGAGCTGTTTATAAGTTTGTCT







TTGAAAAAGGCTTGCACACATCACTCTGAGACCAGCTGTTTCCTG







TGACTTTAACCTTACCTTCCCCAGACAATTGAGCTGAACCTGTAT







CTGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene HAL. In some embodiments, the sequences from gene HAL comprise or consist of:











(SEQ ID NO: 721)



TTATGGTGTCGCTTTATTCTTAGGTATTTATAGCAAAGTTAAGCG







CCTCTAACAGCAGTTAATCAAGCTTCTGAAGGATTCAGTTGACAG







ATGTCAACCGTTTTAAGAACAATATGCTTGACTCAGTACACTCTT







TTTCCCATTGGCTAGCAACAAAAGAGTTGGAGGTATCAGGATTTC







CTCCCCAGCCCCGCAGGCTCACCTGGTCTTTGTCACAAGTTGTCT







CAGGGCAGCTGGAGGAGGCAAACCCTAAAGAACAAGGGCTTTTCA







AGTCCCTGTTGTGCCACGTTCGCTAATGTCCTGTTGGCTAAAGCG







AGTCACGTTCCAGGCCCAGAGTCAATTTGGGAGGGTAACACTCAA







GGGCATGGGCATGTGATTTGACGTGATCCATGGGCCTGTATCTGA







CAATCTACCACATGCATAAAATCCCATCACAGGCTGGGTGCAGTG







GCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGAGTGG







ATCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene HAL. In some embodiments, the sequences from gene HAL comprise or consist of:











(SEQ ID NO: 722)



AGCTACTCAGGAGGCTGAGGCACAAGAATTGCTTGAACCTGGAAG







GTGGAGGTTGCAGTGAGCTGAGATCATGCTACTGCACTCCAGCCT







GGGCGACAGAGTGAGACTCTGTCTCACAAAAAAAGAAAAAAAACA







AAAAAACAAGAAAAAAACCACATAGGTACTGTTAAGACCCTGGAG







CAACTATCCTTCACTCTATCCACACTGAATTCTCTGGGTTTTGTT







ATTTGCTTGCTTGTTTTGCTTAGGAAAAGTATGCAGCTTAAAATC







TTTATGATGACTTTAGAAAGTTTTGGACTTTGTATATTTACAGTC







TTTAATGCAACCAAAGACTAATTGACACAGGTCATGTGTCAAATG







GCTTAAAATAAAAGCAAGCAAACAGCAGCTGTGTCTGGTCATGTA







TAGAGTATGTTTTGACTTCGGATGAAGTTGATGTTTAATAGCATG







TGGAAACAATGTCGTCAATATTTCAACTTTGTTTTGTCCTTGCTT







TTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene NR1H4. In some embodiments, the sequences from gene NR1H4 comprise or consist of:











(SEQ ID NO: 723)



ATTTGGGTGTCGAATGATGCCATGCTCATGATTTGTAGAGCATAA







TAACAATGTTAGCCTTAGGCCATTTTTATACATTATCTTGAGGCA







GAAGCTAGTTGTTACTTTGCTTTACCATAAAAATTATGACTTGTG







ACTAAAAAACTATTCAGCAGAACCTTCTAAAGTTATGTATGGATA







TGTCCTATGACCCAGTGATTTCACTTCTGAGAATATACTCAACAG







AAAGGATGCTCACGTCCACCCTACAGGTTATCTTTAGGGGTTTTG







ATTGGAAGGGGAATTGGGAGGCTTCTGGGAAGCATTACATGTGTT







GTATCTTGATCTCGTTATAATTACGTGTGTGTGCATAAAAATTCA







TCAGGTTGAACACTTAATTTTCACATTTTGTGTACAACATTTAAC







TTACACTTCAAAATAGTTAACGTTGCTATAATTATGCTGAATTAA







TGCTTTTCCACTTTTTAATTTTGTCATTTTATTTTAAACTTCAAA







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene NR1H4. In some embodiments, the sequences from gene NR1H4 comprise or consist of:











(SEQ ID NO: 724)



TTTCTTTGGGAACAAAATTTGATAAATATACAAACTCATTTCTTT







AAATGTTGAAAGACATCCTATTGAATCTGAGGGGGTAATTTACAG







AAAGATACTGTCTCTCGCACTTTTGTCATCTCTGGAAAAGTCCTG







GTGAATAGTCATTCATTTGTAATAGCCCTCTGATCATTTCCTTTT







CTTTGAAGTTCAAAGAGAATAGCAAACAGAAAAGTTAACAATTTT







CACATAAAATATTCCCTCAAGCAATCCTTTCCCCCATAGTTAGTC







TTGAGGCAGGATTCACAAATTCAGAGTATGCAGCTAACTGCAGTG







ATGGGACCTTCGCTTTCCTCTCTTAAAAGTGAGGAAATGATTGGC







TTCATTGTATCCAATCATCTTTCTGTTTTGTTTTGCTTTTGAAAA







CATTCCTACAGCTGGGAGCACAGCTGCTGTATACTTCCCCCAAAG







CAGGAATTAGTTCATTTCCTTCCTTCATTGTTACACTGTTGCATT







CTTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene IGF1. In some embodiments, the sequences from gene IGF1 comprise or consist of:











(SEQ ID NO: 725)



ACCTTTTAGAGAATCTCGCTAAGAAACATGGAGAAAACGGAAAAG







AAAAATGTAATGCCCTAGAAAGCGCAAAGAAAGACAGTGGCAAAA







ATGAAAAAAAAAAATAAAAATTATAAAAGAGGCAAAAAAAGACAC







ACTATTCTCTGCCTCTAAAACACAATTAAATAAAAGAATTTAAAT







AAAAATTAAGGCTTCTATATGCATTTTTAAATTTTGTATGAATCT







GTTATGGAAGAATTGCCTATGTCAATATATGTTCAGAGTTAAATA







TTAGCCCCAAATGCTCAGCAAGACTGAATTGTGTCATAGAAGTTC







CCAGATTCCCTTTTCCCGCAATGTCATTGGAGGCTGCATTTCTTA







GTCAAGTCCAGGGTTTAGGCCAAAGGGCATCCGGTATTGCCTAAA







ACCCTGTGAGGTCTGTGAGGTAACTTTTGAGAAGAGGTCACTGCA







CTCTTCATCTTTTTTGCACTTTGGAATCAGATATAAAAGATGTAT







AAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene IGF1. In some embodiments, the sequences from gene IGF1 comprise or consist of:











(SEQ ID NO: 726)



TGGTCTTAAACCTCAGCTGAGGCTGGAAGAGGACCATGGTGGCTT







ATTGGAGATGTGACCCCAGGGAGCCCCTCTGAAGGATGGAAGGGG







ACTGGGCAAGACCCAACACACACAGAACACAGTAGCCACTGGCCA







GGCAGGAAGCAAGGATCTCAGAAAAGACTTTTAGGTGAATGTGGC







AGGAAAGCGTGCTTGCTGGGGCAAAGGCAGATTCATTCTTTCTCT







TCCCAGGTGACCCAGCGCCTCTTGGTTTCTAACTGGGGAGGGGGT







AGGTGTCAAGAGATGAGTCCCAAAGTTCTGGAATGGTGGGTCTTG







TGACTGAGGTCTAGACCCCTCTCCAGCATGAGTGCTGTCTCCTGC







ATCATATGGAGCCTGGGCATTCTGAGCTCATTCAAAGGGACACCA







TGGGAACCACTTGTTCTCAATGCAATTATTTTTGTGATGTTTACA







GTATCAGCCCCCATCTACCAACAAGAACACGAAGTCTCAGAGAAG







GAAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene HPD. In some embodiments, the sequences from gene HPD comprise or consist of:











(SEQ ID NO: 727)



TACTGTGTCTGTGTTTGTGTTGCGCATAAGGCAGTAGTGCCTTAT







GGCAGCCAGGGGGAGGCCAGGGCGGCCGCCAGCGCCGCGGGACCG







AGGAGCTGCGGGTGCCAGCGCTTTGCCAGCGGGACCACGCGGAGA







GGGGAATTCTGGAACCAGGGAAGGAAGGGGACTTGCTCTGGAGGG







AAAGACGGATGACACTGCTTTTGTAGAGAGATAAACGCGATTCAG







ACAATAAAGCAAGCGATGAGCCGGGCGTGGTGGCTCACACCTGTA







ATTTCAGCGCTTTGGGAGGCAGAGGTGGGAGGATCGCTTGAGGCC







AGGAGTTCGAGCCCAGCCCGGTAACATAGCAAGACCCCGACCCCC







CTGCGTCTACACAAATAACAAATAAAAATTAGCCTGGCGTAGTGG







CGCACACCTGTGGTCCCAGCTACTCAGGAGGCTGAGGTGGGAGGA







TCGATTGATGGACAGAGGTCTAAGCTGCAGCGAGCCCTGATCCTG







ACAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene HPD. In some embodiments, the sequences from gene HPD comprise or consist of:











(SEQ ID NO: 728)



ACAGCGCAAGGCTCCGTCTCAAAAAAGAAAAAGAAAGTGTCCTGA







TAATGTCTGCGGGGAGGATGGATTGTAAGGATACAAGATTAGAAG







CGGGCAGCAGTCTTGAATGGTGCCTTAGGAACTGTAGTAGGGGGA







TAGTAATAGGACAGTTTGGGGATATATTTGGAGGTGGCTGAACTG







CCAGAAAAAGAGGTCATTTCCAGACTTTTGGGGTGCAGGAGCCCC







TGTGGACTTTCCAAGGATCTCACCGTGCCAGCCTCCCCTAGCAGA







GCCCCCTGTCTCCCGCCTAGGAGCTGAAAATCCTGGTGGACTACG







ACGAGAAAGGCTACCTCCTGCAGATCTTCACCAAACCGGTGCAGG







ACCGGCCCACGCTCTTCCTGGAAGTCATCCAGCGCCACAACCACC







AGGTACTGCTTGTCCCCGGCAGGCCCGAGGGGACAGGCAGCTAGC







ACACTCGGTTTTCTGGGTCCTTGGCTCATCTCCTCTCTTTCCCGC







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PHF11. In some embodiments, the sequences from gene PHF11 comprise or consist of:











(SEQ ID NO: 729)



CTTCTCCAGTCGTTCCAGTCCTCCCCGGCCCTCTACTTGAGTGGG







TGGGTCTTCTGATGGGTTCCTGCCCTTGCGTGTGTGTATAGCTCC







AAACGGTGTGCCAGGCTCAGGGCAAGGTCCTGAGCATACAAAGAT







GAGTTAGGCAGACCCTGCCCTAGAGACTCTACTAGGCAACTTTAA







TGAGGAGGTCAGAACCACAGAGGTACAGATGAGAGACTCCTGACT







CCCAGTCTTCAGGGAAAGCTTTTTGAGGAGAGGATGGCTGGGCTT







ACCCTTTTTTTTTTTTTTTTTTTTTGAGAGGAGTTTCACTCTTGT







TGCCCAGGCTGGAGTGCAAAGGCGCTATCTCGGCCCACTGCAACC







TCTGCCTCCCGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAG







TGGATGGGATTACAGGCATGTGCCACCACGCCCAGCTAATTTTGT







ATTTTTAGTAGAGAGGGTTTCTCCATGTTTCATGGGCTTACCTTT







AAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PHF11. In some embodiments, the sequences from gene PHF11 comprise or consist of:











(SEQ ID NO: 730)



TAAGTCACGGGAGATGCGCTTCCATCCTGACCTCTTCCTCAAACT







CCAGACTGGTATAACCAACTTCTTCCATTTCTGAAATGATCTCAA







ACTGAGTATGTCCCAAAGAGAATTCTTGTTTCCTCCACTGTATTC







AGGTACTCCAAACCTGCTCAACCACCAGCCCATTCCATTCGCCCA







GGTGCTCCTTGCAAAAACCTTAGTGGCTCCTCAGGGCCTCTCTTT







CCTCCGGACACCCCTTCTAATGCATCAGTCGGGCCAGCCAGTTCT







GTGTTCAGCCTGACTGTATCTGGAGCCTGACTGCTTCTCACCACT







TCCATTACTGCCACCCAAGCCTACGCCATCATGATCTTCTGTCTG







GACTATTGCTTTCCTGGCTTCTATTCTAGCCTTTAATACAGGCAG







GCCTGCAGAGCGGCCAAAATGCTTTTTGAAAAACAAAAAGCAAAT







GTGATTCTCCTACTCAAAACCCACCAATAGTCTCCCATGACTCTC







AGAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CCNB1IP1. In some embodiments, the sequences from gene CCNB1IP1 comprise or consist of:











(SEQ ID NO: 731)



TTAAGTATCATTTGTTACTATTATTTTCTCTACTGAGCGTGGAAA







GCTGGCTGAAAATTCCAGAGTGTAATTTCTGTGGGTCTGTTTCTC







TAAAACTTGCCCCTCACGCAGCTTGTACAGAAGTTAGATTGGCGC







TTACTCCCAGCTGGTGGAAGATTCGGAATTAGCTGCGCCTCTGCT







GCGTGAGAGAGGGCTCAGCTTTAAAGCTTCCCAGCTCCTCTGCTT







TCCCGCTGCCCCCTACTCTGCCGGATGTAAAATAGGCCTCTTAAT







CTTAACTGTGGCTGCCAGGTGACATACCTGAGGAAACTCACTCAG







CGGGGCTATTGGGATAAAGGTGTGCGAGTGTTCTTTTCTATCGAC







CTGGAGGGCCAAGGCAACTTTTTTTTTTTTCCTTCTGGGACGGAG







TCCGTCGCCGGCTGGAGTGCAATGGCGCGATCCCGGCTCACTGCA







AGCTCCGCCTCCCGGGTTCAAACGAGTCTCCTGCCTCAGCCTCCT







GAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CCNB1IP1. In some embodiments, the sequences from gene CCNB1IP1 comprise or consist of:











(SEQ ID NO: 732)



AAAGACGGAGGGTCGCTCTTGTTGCCGTGGCTGGAGTGCAATGGT







GCGATCTCGGCTCACTGCAACCTTTGCCTCGTGGGTTCAAGTGAT







TCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTGCAGGCGTCCAC







CACCACGCCCAGCTAATTTTTGTATTTTAGTAGAGAGAGGGTTTC







ACCGTGTTGGCCACGCTGGTCGGGAACTCCTGACCTCAGGTGATT







TGCCCGCCTCAGCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCC







ACCGCGCCCGGCCCACTATCGCTTAATTTAAAGAGCTGTAATGCA







TGTTTGTAATGCAGACTCAAGGAAAATTATAATTTCCTTAAATGT







GTATAGAGGATGTGTGAGAGTGGAAAGATGTCTATTCCAAGGTGG







ATAAAAAATAAAATGGTGGACTACCTCAACATGGAGGTGCCAGCT







GTCCAATTACAGCTGTCACTCTGTTTCTGGGAATTTCTCCCGATT







CTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PSMB5. In some embodiments, the sequences from gene PSMB5 comprise or consist of:











(SEQ ID NO: 733)



AGGACTACTTGCATGACCAAAGCCAAGATGGCAGGTACGTCCTTT







TAGAATTTAAGGTTAGTGAAGCCATCTATTTCCCCGACCCCCTTC







AGTGAAAATGGTCTTTCGCATCTGGCCCTTCTTTTTGGAGGCGTG







CTTGCCAGCAGTCAAAATGGCTCCATTCCGGAATAGATTTAATAG







GAAGTGAAGCTGTGACGGCGAGGCGTTGCCCGGCCTATCTTTGCT







AGGCGTTCTCAGAATTAGTTCTTTCTGCCCACACTAGACATGGCG







CTTGCCAGCGTGTTGGAGAGACCGCTACCGGTGAACCAGCGCGGG







TTTTTCGGACTTGGGGGTCGTGCAGATCTGCTGGATCTAGGTCCA







GGGAGTCTCAGTGATGGTCTGAGCCTGGCCGCGCCAGGCTGGGGT







GTCCCAGAAGAGCCAGGAATCGAAATGCTTCATGGAACAACCACC







CTGGCCTTCAAGGTGTGGAGCCAGCCCCCTTGCCAGGCTGAGTAC







TGAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PSMB5. In some embodiments, the sequences from gene PSMB5 comprise or consist of:











(SEQ ID NO: 734)



TCTCAACCGTCCTCCCGTCTCTGAGATGGTAAATTACTACTGACA







CTTATTCAGGTCTGAACTGAGAATCTAGTTTGTGGATTAACTGGA







CCCTTTGCTTTTTCTTCTTCCATCTGCCTTTTAGGCAATTCATTA







CTTATTCGAATTTCCACCAGAAGGTAGGAGTCAAGAGAACGAATA







TTGGATATTAATAACCGGTTACTGTTAAAGAAGGGTTGCTGTATG







GCGACTGACAACAAGAGGTATAGCACTTTTATTTTGTTGCGTTGT







TTTACCACTAATTGCTGTGGTTTAACTAATATAGGGAAATGGCCT







CAGAATGGATCATTATTTTACCCAATTTTAGAAGGTGCCCTTAAA







CAAGATGGCAACCTCAGGCGCGTTAACAAGGTGCTGAAGCAGCAG







CGATGTAACCTGTTGCTTGGACACCAGGCTGGAGTGGGAGGACTC







ACCGCTAAGGGTTCAGAACCGATTTTCAACCAGTCACGCCCTTCT







CAAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CTAGE5. In some embodiments, the sequences from gene CTAGE5 comprise or consist of:











(SEQ ID NO: 735)



AAGTCCGGACGTGGTTGGCAGGGCGCACCGGCGCGTGCCCCGCAG







GCCATTTTTCTGACTGGAATGACGGCGGCGGCTGGCTTCTCGGGG







CTGCCGGGGTCTCCCGGGGTACGCCGCCGTCAGGACTTCTGCAGG







GCGCAGACAGCAGGCTCCAGCGAGGCTGCGGAAGGAAAGCCTTGG







GTGTCGGGGCTGGGCCTGGCGTCGGCCCCTGGGTCGGGGGGGTGG







GGGTTGCGCCGAGACGCCTCTAGGAGAAGTCTCGCCGCCGCCGCC







ACCGCGGCCGCCCGAGGCGGGTGCCCTTGGGACATAGCCCTGGTC







TTTGGGGTTGTGCGGCTCACACGACAGCGCGGAGTATGAGGCCGA







ATCTCATCCTCTAGTCCCAAGCCTCTCCACTACCAGGGCTGGGTG







GCTTCGCCAGGTAGAGCGCCGGCCCCTTTAAGAGCAAACGACCCG







GACACGTCTGCGAAGCTTGCGCGAAGAAGGGGAAGTTTGCGGCTG







TCCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene CTAGE5. In some embodiments, the sequences from gene CTAGE5 comprise or consist of:











(SEQ ID NO: 736)



TGAGCCCAGGCGCGATGAGTGTTACGTGGCCCAGGGGTCGCGGGA







GCCGCCGGGGGAAGGAAGCAGTAGACCGGCTTTGGGGTCTAAGCC







GCCGTGGTCAGAGGTCCCGAAGCCAGTCCTCGTCTGCTGCCCGGC







TCCCGCCCGTGTTCGAGGCAGTAGTTAGGCTCGTAGGGCGCTTGA







GTGGTTTTTGTGTGATGGAGGAGGACCTGGGGCTCTGGGAAGGTG







AAGAAAGCACTTGCTTAGCTGGACTTTGCAAAGATGTAAATGTTT







CCCGATTCCTAAAACTAATTAGCCTTTCCGCTATGTTGTTTATTT







TGAGCAGAGACACGTGGTTCCTTCGACTGTGAGAAACTTGAGGAC







TGTTCCTTTAAATTAGTTACATTTACTGATGTAACTTCTCGTTGA







ACCTTGAAAGAACACCTGAGGTTGGGCGGGGAGTTGAAATTTTGA







TTCTGCATTTTTTTTTTTTTTGGTAAAGAATGAATGCCCTTTTGA







GATG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SERPINA10. In some embodiments, the sequences from gene SERPINA10 comprise or consist of:











(SEQ ID NO: 737)



GTACTTTGATAATGTTCTGCTCTCCCAAGGCCACAGGGCCCTACG







ATTGTCTCTCCCTTTCCTTTCGTTAGGCCAGCATATGATTAACGC







TACGTGATTTTCTATGAATGTGTTTTCACGTTTCAAAAACAGATT







GATACACATATTGAACAGTGCCAGACGCTGTCATTTGAGGCCCTT







CCCTGGTATCCTATGTGCTTGTAGTCCTTATTATTTTCAGAGCAC







TCTACATAGCTCCCCTCTGACACTTAGAAGCATAGGGTCTTTCCA







AAAAACAGGGGGCTGGGGGATTATCTGGGGGATTTAGGATTGCAT







CATTGCTCCTTCATTTTTACTTTTTGACCAACTCTCTGCCCTTAG







ATTCCTATTATAGAAAATAGGGACACTCCACCTACTACAGTGTTA







GAGGCTAAATGAGACAATGAATGTAAAGTGCCCAGATGGGCTTGG







CACATAGCAGACACTGAGTATCTATTGTTTACTTGTTCTTCCAAA







CTGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SERPINA10. In some embodiments, the sequences from gene SERPINA10 comprise or consist of:











(SEQ ID NO: 738)



TCCCCCTTCCTTGCCTAACGGGCATGCAGGCACTCCAATCCCCAG







CCAAACAGGGCACTGGGCAAGGCCGGCCACCCATCTGGATGGGCA







GCCTGACGACCAGATGGTCAGGGCAGTGAATGAAGCAGATCAAGG







AAAGGTGTGTGAGGACCCCTGATTCCACCTGCTTGGACCCCCACC







TTCTGTGCTGCCTCCTGCTCCCAGAGTGGACTCTCTTGCCCTGGC







CCTCAGGGAGGAGACGGGATGAATGAAAACGGGGTCAGGACTGAG







AGCTGCCTGCCGGCCTGGCAGGGAATGGGAACTGGAGGAGGTTTT







GCTCTGTGAAATAATGTCCCCTCTTTGGGTGAGCAAATGTCACCC







ACACTTGCTCTAGGTCTCCCTGGGGCAGGGCTAACCTACTTGAGC







CACAGGAAGGAGGCAGGGTCCCTGAAGAAGCTTTTACTATCCACA







AAGACATTTTAGGAGGCATTAAAACCATCTCTATCCTCTCCTCTC







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SERPINA5. In some embodiments, the sequences from gene SERPINA5 comprise or consist of:











(SEQ ID NO: 739)



TCCCCTCATTCTTCTGAGCCCCAATTCAGTGATTGGTGCAATCAC







AGGCTTGGCTACAGTGACCCATTCATTGCAGGCATGGTGAGTCTC







TCAATCCCTCTCATTTCCACTAGAATCTAACTGTTGGGATCTATG







ACCCAGTCAGCATAGCAGGCCTGTGGGGAGCTCTCAGGTTCAAGC







ATATGCCCCCCCTAATCTACAAGAAATTAGCTGCAGAAAACCAAG







GAATAGAACCTGGAAAAAGAGAGGGTTTGCTAGAGCTGTCCCTTT







CCCTGTCTCTGGAATGCCAACAATAGGGAGGCTCTTTGGTCTTGT







CTCTCAGGAGTGCCCATGCCATTCCAGGAAAATGATGGCCCAGCT







GGTGGTGTAAGGCTTGGGGGGCAGCGAGTGGGCATCGTGGTGAAA







GCCTCGGGATCAGGGAGCTGCGTCTGCAGGCAGGCCTGCTGGCCG







GAAACCTGCCAGGAAAGGAAGGGGCTGTCCCGGGGCGGGGCCAGG







GAGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SERPINA5. In some embodiments, the sequences from gene SERPINA5 comprise or consist of:











(SEQ ID NO: 740)



TAAGTACCGATGCCGCAAAGGGAGGTCCCCAGGGCTTGAGGGCAT







GTGAGGCGAGGAGAGGATGGACTCTAGAGTTTTGGGGTTTGGGGT







CTGCAAAGCTCTGAAGGAGTCTCATCTCTGCAGTTTCAGGTATCC







AAGGCAGCAGAGGTGAGTGGGTCCCCCGAGCTCTGTGACCTTATG







CTCCACACTAACTCTGGCAGAGCCTCCGTTTCCTCATAGGTAAGA







TGGAAATAATTACACCCTCTGGATGGTGTGACTGAAGATTAAATA







CAGCGGGTGCTCTCACTCAGCACATCTGGCCATGTCTGCAGACAC







ATTTGGTTGCCACAACTGGAAGGGGGGTGGGGGTTAGTGACATCT







AGAGGCCAGCGATGCTGCTGATGATCCCACAATGCCCAGGACAAG







ATCACAAAGCATCATCCTGTTCAAAAGGTCAACAGGATCAAGGTT







GAGAGACCCTGAAATAAGGCCATGGGGACAAAATGTCGGCTGGAT







AGGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PPP2R5C. In some embodiments, the sequences from gene PPP2R5C comprise or consist of:











(SEQ ID NO: 741)



AGCGTGGTGGCGGGTGCCTGTAATCCCAGCTACTAGGGAGGTTGA







GGCAGGAGAATCACTTAAACCTGGAGACGGAGGTTGCAGTGAGCC







GAGATCACGCCACTGCACACCAGCCTGGGCAGCAGAGTGAGACTC







TGTCTCAAAAAAAAAAAAAAAAGTGAGCAATATCTGTGGTTAATC







TCAAATAGGGTGGCTTTCCGTGTCATTTCCCTTTAGTTGTTTTTA







AATGCAGATGTTGCTTTAATTAAATAATATTTGAAATAAGTGGGG







TCCCAACTATAACCTTATATCTTATTGTTTTTAAATGTTGATATT







TTGGTGTTTTCAGTAGAATTTGAGCTGTTAACAGTGATTAGGTTT







TTTCAGTTTGTCATCGATGTATGTGACCTCGTTATAATACACATG







GATTTTTGTCTGCTGGTTGTGTTACTCATGAGCAATGTGTTCTAA







TTATGGTATTTTAACTTATAACTTATGAAAAAGTTATGTTTCACT







CTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PPP2R5C. In some embodiments, the sequences from gene PPP2R5C comprise or consist of:











(SEQ ID NO: 742)



TGGGTGGTGAGGGTGTCTGTATATGTGTGTTTACATTTGTAGACA







GTTTCTGTACATGTATAATTAGGAATGGGTTGTCGGTTTTGGTTT







TTTTCCCAGCTTTATGTAATTGGTCAGACTTCTCAAAGGGGGGTA







GCACTTGATAATCGCTCTCTTGGAACTCTGAAGCCTTTGCATGGA







GCAGTGTTCACCCTGGGCAGCCTGTGGCCCCTGGGGTCACAGAGC







TGCTTTCCAGGGGGTTTGCAAGGCCGTCACTGCCTCATAAGATTA







CCAAGATCTAATTTACCTCTCACTGTGTGGACGTTTGCCCTGGTG







GTGCAAAGGCAATGGTGAGGAATATGGCGCCTTTCTTTCATCGAG







CAGGACAGCGGTGTTCAGCTAGCAACACACCAGTCTGACCCAAGA







ATGTCCTCGATAAAGCATGAAGTTACTGGTTTTTTAAGTCTCAAC







TCTTTTGTATACCTTTTTAATTTTGTTTTTGTTTGGGTTTGTTTT







TAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SERF2. In some embodiments, the sequences from gene SERF2 comprise or consist of:











(SEQ ID NO: 743)



AGGATCCGGCGTCCGGAGCCCGGGATCACCTGCCTAGCGGGCTTC







ACTTGGAGAGGTGGGGAGGCCAGAGCTTGCGGAAGCCGCTCCCTC







TCCCCTGCACCTTCTCCAGGGTCGCCGGCTGAGGCCCAAGGTCCC







TAGGGGCAGTGACTAGAGAAGAAGGGGGCCGGCCGTACCTGTTGC







CGCTGACACTCTTGCAGCGCCCGCAGGGCAGCGTCGTTGAGCCTG







AGCAGTAAGAGGCTAGTCCGGGCAGCTTGCGTGAAGCAGAGCCGG







AGCTGTCCTCTCAGAGGCTCCTGGAGCTCCTCCATGGCGACGAGT







TCGAGTGCAAGCAGCACGGGGGCCACAGGCGAGGGCCACCACCGC







CACCTCCTCTGTTCAGGGTTTGGTTGGCACACCCGGTAACCCACG







TCTGCCCCACCCTCCGCCAGGAACCCGCCCCCGGCCCCACCTCCC







GCCAGCGCCACCCCAAGCTTTCCCAGCCGCGTGGGCGGCGCTTCG







CTCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SERF2. In some embodiments, the sequences from gene SERF2 comprise or consist of:











(SEQ ID NO: 744)



TAATTGGGGCCCTGCGGTTGTGGTGGCAGCAGTGAAAGTTGGCTG







TCACCGACTGGAGCGCCCCCAGTTGGTGGGCAGGGATACGGGCGC







GGGTAGTGGCAGGAAAGTTGCAGGGCAGAGAGCCAGAACCCAGTC







CTAGGTCTCACCTCGTGGCCTAAACACGCGTCAGAGTGGTGAGAC







CGCTCCCTGAGGAGGGCCAGGAGCTGCCATGAAGTCGCCAGGCAT







TCCAGAGGGCCAGATCCTGGTGCACTGAGACCCCAGCACCAACCT







CTGGCCAGCCCTTGGTGTCTCCACTTGTTCCTGTTTGATTATGTT







TCCCAAGAGCCCCGATGTTGTCCTGTTTCCTAAACAGGATCCCTC







TGTTAAATCCCTGGACCTGCACAATCCCCGGGAACCTTAACGTCC







TCTACTACAAATAAAATTACCTATACATTTGTCAAAACTCATAAA







AAAAAAATACATTTTTTTTTTTTTTTTTGAGACGAAGTCTTGCTC







TGTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene AQP9. In some embodiments, the sequences from gene AQP9 comprise or consist of:











(SEQ ID NO: 745)



GCCCGCTGCTGTGGCAAGAATGGCCTGGGGTCAGTGCTAGCTGTG







GCAGTGGCTGCAATACCCCTGGCTCTAAGGGCATCTTCAGTATAC







TGCAACACCCTCAAATTCTGGAGTGGTATTAGGGGTTACTGAGAG







CCCTCTGGGAATTATAAATTATTCCTAGAGCAAGACCAAGCCCCA







CTTTGGCTTACAGTTGACCTCGAAAACTCCAAGAATTCCCCCAAA







AGAGAACTCATTCCCAGGACCCTATCGAAGAATAATTTGTCCAAG







TTTGCAGTGGGCCATTCAGAGAGGCCAAAGGCCTTCTTCCTTTGA







CCCTCCATCCTGGGATTCACCTGGGCATCTCTAAAACTATCATTC







CTCGGAAGTAGAGAGAGACAAATGACATGGCTATGCCAAGCTCAA







TCTGAGTGACTGGTTGTTTAGCACAAGGCTTGGCACAGGCCTCCT







TCAACTCTTCCCAGGGAACACTAATGTGCCAGAGCTTTCTCTTTC







ATAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene AQP9. In some embodiments, the sequences from gene AQP9 comprise or consist of:











(SEQ ID NO: 746)



TAAGTGTAGATTCAACAAAGACTTAACTTTGGTGAAAAGATATGC







TCTCATAAGGGGAATGCATTGGAGAATTAGAGACCAATCAGAAAG







GAGAGATTATATTTCCAAAGGCAGAGGTTGCTGGGCATAACCCAA







GCTTTCTTGATAATCGTTTTACCTTTACAAGGAGCTGATACCACC







AGAAAAGTTTTCTCCTTCCTCTTTAATACGTACACTTGTTTAACA







TTAGTTGAGATCCAGAAAAGAGTGAATGACTAAACATTTCAATCC







CTGTAGACACAAATGGACTTCACTTCACTATTGACTCTTTTGAAC







AAGTGGTCTAAGTGTGGAGGCCACAAGAGAAGATATTTCTATTTC







AAATCAAAGACTGTGAAGGGTGGTAGTTGAGCCTAGGGGATCATC







AATCCTCTCTGTCTCACCATGTGCATAGGTTTATTTATTTAAACT







CTCAAGGTGGCTAGAAGTATCTCTGCTTATTGACCTTTAAACAAG







CTTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ANPEP. In some embodiments, the sequences from gene ANPEP comprise or consist of:











(SEQ ID NO: 747)



GTAATCCAGCCCTGCCTTATGGGCAGCAGCCCCCACCCCATACTC







CTAGCTCCTGGCCCATGTTCTGAGGGCTGTGATGGGCCATTCCAT







TCAACAAATGGACTTGACTTCTTTGGTTCCAATCTCCACTTTGCT







GCTGCTTCGCTGTGTGACTCTGGGCAAGTTACCATCCCGCTCTGG







TCTCTAGTTCCTCTTCTATAAAATGAGGCTAAGAGCAATACTCCA







TTAGTTATGTGAGGATTAAAGGAGAGAATCCTAGGCCAGGCATGG







TGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGACC







TGGGGTCAGGAGTTCGAGACCAGCCTGACAAACATGGAGAAACCC







CATTTCTACTAAAAATACAAAATTAGCCAGGTGTGGTGGTGCATG







CCTGTAATCCCAGCTACTCTGGAGGCTGGGGCAGGAGAATTGCTT







GAACCTGGGAGGCAGAGGTTGCAGTGAGCCAAGATCGCACCATTG







CACT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ANPEP. In some embodiments, the sequences from gene ANPEP comprise or consist of:











(SEQ ID NO: 748)



CGAGAATCTGCAGGAGATGCCCTGGATGGCAGGGACTGGAGGGTC







CCACTGATGGCCACGCCCTCTCGCTCCACCCACAGGCTGTGAACA







ACCGGTCCATCCAACTCCCCACCACCGTGCGGGACATCATGAACC







GCTGGACCCTGCAGATGGGCTTCCCGGTCATCACGGTGGATACCA







GCACGGGGACCCTTTCCCAGGAGCACTTCCTCCTTGACCCCGATT







CCAATGTTACCCGCCCCTCAGAATTCAAGTGAGCAGATGGGGTCA







CTGAGGCATGGTCAGGTGGGTGGGGTAGAGGGCAGTTACTAAGGG







ACCATAGTAGGACTGGCCTCCTGTGGTCCCAGCCCCAGCCACTGT







AGACAGACCCTCCCTCCAGAGCCAGGAGTTCAGGGGCACTCGGTG







CCAGAGCTGGGCAGAAGCTGGGAGCCTGGGCAGCTGCTCCTCACT







GGCAGTGACCACACCGCCATCTCTCACCACCTCTGGATCTTTAAT







TGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PCSK6. In some embodiments, the sequences from gene PCSK6 comprise or consist of:











(SEQ ID NO: 749)



ATGAAAATGAAAATGACAGATCTGAGGAAGAGGGAGAGCTCTCTT







GAAACCCCTTTCCCAGATTTGCCCTCAGTTTTAGGAATGAGGTAC







GCTTGGGCATTCCTGTTGCCTGCATGGTTCGGCATTCTGGCAGCC







AGGTTCGCCCCAGAATAGATAAGTGTGTTTGATTTTCGAACATTT







GACTTTATTACCTATTTCCAGAAACACTTCGTGTTCAAACTACGT







CTCTACAGGCATTTCTACTTGGTCTTTTTCTCCCCTGACATTTCA







CAGAGTCCTAACTGTGCTGCATGTATAACTTGTTCTTTCTTTTTG







TTACTTGCATAGCAAGCATGTTTCTATGTCATTAAATATCCTTTG







TAAGCAGCATTTTATAGATTTTTATAATACTTCACCAACAGAAGC







ACCATAATTTATTTAAGCATCTCTCTATTGTTGGACATGTAGATT







GCTTTCCATTTTTTCCTATTATGAGCGGACAGCTTAGTACAAAAA







GCCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PCSK6. In some embodiments, the sequences from gene PCSK6 comprise or consist of:











(SEQ ID NO: 750)



CACTTCCTTGCCTCGGCTCCCCCAGCCTCCCCCACGGTGAAGCCT







GGGCGTTGGAATGGCTGCTGACGTTCCCACCGGGGAGGAGCAGCG







GAGGCGCAGGGGCCGGTCCGACCCAGCGGGACCCCGGGGGGGCGG







GGGTTGTGGCTTTTCCCTCCACTTCCAGGTGAGGCTTGGTATCAG







GCCTCCCCAGCATCTGGCTGCCTGGAGGGGGCAGGTAACACAACC







CAGAAGGAAGGTTTACCCAAGGAAAGACAGGACATAAAGTGTATG







CCCTTCCTGTCTGAGGGCACATTGGTCTCACAAGGCCTGTTTGGA







AGACTTCCCCCAGCTAAGCAACCACCCCGTTTCTTTGTGGAGCTG







CAGCCAGCTTGAAACACAGCTCCTTCCATTTGTCAACCCCGGGCG







CTTCCTCCTGCTGCCCTCCACTCCTGTTGCCCTGGGATTGCACCT







CCCAATAAAGCTTCAGCCCATGGGCTTCACTTCAGTCCCTTGTTT







TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ACSM5. In some embodiments, the sequences from gene ACSM5 comprise or consist of:











(SEQ ID NO: 751)



GTTAACTATGGAAAGAAAAGAAGAAAGACAGGCAAGAAAGAGGGA







AGAAAATAGGAAGAAAATGAAGAAGGAAGGGAGGGAGGAAATAAA







GGAAAGAGAAAGGAAAAGAAGGAGGAAAGGAAGGGAAAAAGATGA







ATGGAAGGAAGGAAATAGGGAACAAAGAAGAAGGGAGGAGAGAGG







AAAATGGAGAATGAAATAACGGGGAAAAGAATGAAAAAAAGGAGG







AAAGAAAATGAAGGAGGGAAGGAAGAAAACAGGAAAGGGGAAAGA







AAGGAAAGAAAGAGGAAAGGAAGGAGTGAGCAAGGAAGAAAAAAG







AAAAGAAAGAAGGGAGGAAGAATGGAGAGAAATGGAGAGAGGAGA







AGGGAAAGAAAGAAGAAAGTGAGGGAGGAGAAAAAAGAGGATGAG







AGAAAGAGTTATCAGGCAGGGTGAGAGAAAGGCATGGCCCAGGAA







AGAAGCTAAGTGGAAAGGCTCTTCTTTATCTGTACTGCGTTCTCC







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ACSM5. In some embodiments, the sequences from gene ACSM5 comprise or consist of:











(SEQ ID NO: 752)



TAGCAGCCTCCCCCAACTTCTGGCAAGGCCTGGCATGGAGAGGAG







AAAGACACACAGGCCTGGTTGGCACGGGCTGCTGGGCTGCTGGGA







GAGGCCCAGGGTGTAGACACTCACGTATGCACCTCTGTGATGATT







TCCTTACCAGATTGTGGATGATGAGGGCAACGTCCTGCCTCCTGG







AGAAGAGGGGAATGTTGCCGTCCGTATCAGACCCACTCGGCCCTT







CTGTTTCTTCAATTGCTATTTGGTAAGAGACGGGGAACAGCCGTT







CTCATGACAGTGACTGTGTGCTAAGCATTGTGCACCACACTTTGT







AAATATCTATTTGTTTAATCCTTACAATGACTGCATGAGGTAGGT







TGCTACTATTATTAATAAAGTTCTGTTATTGATAAGGAAGACTAT







TATTGATAAGGAAGCTACTACAAAGGGGAAGTGACTTATCCAGCA







TCATGCAAATAGTACAGGCAGGACATGGGATTTAAATGTGGGTGT







GTCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ACSM3. In some embodiments, the sequences from gene ACSM3 comprise or consist of:











(SEQ ID NO: 753)



GCTGTGGATCAGAGGACCCTGTGCCAGATCATACAACTCCTTGCA







GTTCATGTAAGGACTCGGATTTTACCTGGAGTGGAAAAAGAAGCA







CTGAAAGATTTGAGCAGGGGAGTAACCTGATAGCGTTTATGTTTA







GTCCTGCCACTTCGACAGATAAACGCACCAATGGGCTTGATGAGA







TTTAGGCCAACCCATAACCGCCCCTCAACTTCTTTCCTTTCAATT







TCAAAACTCCTCTATGGCTTCCTCCATCTGTTCTTCCTTCTGAGA







AGTGCTCTCTCTGCCCCTTTACAGAACTAACCACTTCGGCAACTC







CTTGGACACTTTCCTTCTTGTTAATAATTTGCTTTCTCCGCCCCT







CAAAAGCTTGCTGTTTCTGTAAATCATTACCTGTAAGAGGAACCG







CTGGGAGTCCTGTAAACTTTAGCCCAGAGCTTGGCTCCTCCTCCA







GAATGTCTCCACCAATCAAGGAAAGTGTTTTGGGCCAGTCTTGCT







CCTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ACSM3. In some embodiments, the sequences from gene ACSM3 comprise or consist of:











(SEQ ID NO: 754)



TGAGTACCTCATCTCCCCTCCTGTGTAAGATTTCCTGAACTGGCA







CATCTGTTTTTTGAGCAAAGATAACAAACAGATGAACAAAACCAA







CAATCAAAAATGCTGTCATTAAAGTCTTGGGCAGCCAAAGTTTCT







CTCAGAATTTCTCAGTTGTGTGATACTATCTATTAAGTGATGAGG







AGTATGCACACACAAAAGGCTATAAATGTAGCAGCTGAGTTTTCA







TGTTGAGCCTTTTGGTGCTATTTGATTTTTTGAAAAACTATGTAC







ATGTATTAAGTTGATAAATTTTTTTTTTAATTTTAATTGAACCAG







ATGCGGTGGCTCAAGCCTGTAATCCCACCACTTTAGGAGGCTATG







GTGGGCAGATGCAGATCACTTGAGGCCAGGAGTTCGAGACCAGCT







TGGCCAACATGGTGAAACCCTATCTCTACTGAAAATAAAAAATTA







GCCAGGTGTGGTGGCAGGTGCCTGTAACCCCAGCTACTTGGGAGC







CTGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene TMED6. In some embodiments, the sequences from gene TMED6 comprise or consist of:











(SEQ ID NO: 755)



GTCTGTTTTCATTACCATGGCTATTATAATAATCATAGGGTCCTA







TGAAATTACAGAAATAATGAGGAAGTATACAGAGATGTTTTAACA







GTAGGAAAAAAATTCAGCCTTTAACACCAAAAACCAGTGTTCCTG







GAGGCTGCTGACCTCCAAGGATGCCAAGTGGCAGTCTACTGACGA







TTTGGGGGCAACACTCCAGGTGAGCATTCTAAGTCTAGGGTTCAG







CCGCTCAATTTTATCTCATCATCTCCAGTTAATCAGATTCTGGGA







AAGTGACCAAAAAACAGAGACCCATTTATAATACAGAATTCCAAT







TAACAAATGGATTACAGGGAAGCATGTCTTTCCAGTCATCAGAAT







ATTTGAGTCAGTTACTAAAAGCAGATTCAGAACAAAAGAAAATGC







ATGATATCCATGTGAGATATCCACACTCCACAGCAAAGCACTCCT







CCAGCCAGGTCAAGCCTGTCCCACTTTGGAGAGCAGCAGTGTCTT







AACT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene TMED6. In some embodiments, the sequences from gene TMED6 comprise or consist of:











(SEQ ID NO: 756)



TATAGGCGCACGCCACCATGCCAGGCTAATTTTTGTATTTTTTAA







GTAGAGACAGGGTTTCACCATGTTAGCCAGGCTGGTCTCGAACTC







CTGACCTTGTAATCTGCCCACCTCGGCCTCCCAAAGTGCTGGGAT







TACAGGCGTGAGCCACCGCGCCCGGCCCAGAATCATACATTTTAG







TGGATCCACACTAGCTGTGTGTTCCGAGCAAGGCATGTCTTCTTC







ACACGGGTGCTGTTGGCATTCTGGGCAGTTCTTTGTCCTGCTGTA







CTGTTTCTTACATTGCAGACGTTCAGCAATGCTGTGCTGCCTCCC







CTTAACACCATCACTGTGATACCTCAGAATCCTCCAATCAACTCC







AAACCCCAGCAGGGGGTTGGGCAGTCCCACCCCAGACAGAGACCG







CTGTGCTGATGACCGTGGGACGTCAATCTTACCACTCAGCTGGCT







TCCTCGTGATGTAGCAGGGGTTCTGCTACCTATTTAATGTGTCTT







GGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene HPR. In some embodiments, the sequences from gene HPR comprise or consist of:











(SEQ ID NO: 757)



AGGGGGCTGGGCGTGATGGCTCACACCTGTAATCTCAACACTTTG







GGAGGCTAAGGCGGGTGGATCACTTGAGGGTGGGAGTTTGAGATC







AGCCAGAGCAACATGGAGAAACCTCGTCTCTACTAAAAATACAAA







AAATTAGCCAGGCATGGTGATATATGCCTGTAATCCCAGCTACTC







GGGAGGCTGAGGCAGGAGAATTGCTTGAACCTCGGAGGTAGAAGT







TGTGGTGAGCTGAGATCATGCCATTGTACTCCAGCCTGGGCAACA







GGAGTGAAACTCTGTCTCAAAAAAAACAAAAAACAAAAAAGAACA







CCACGGGAAAATCAAGTGTGAAGCAAGAGCTCAACTCTTAACAGG







GGCAATGTTTGGAGTTTTGTTACTGGAAAAGATAGAGACCTTACC







AGGTCCAAAGTTTGTAGACACAGGAATTACGAAATGGAGAAGGGG







GAGAAGTGAGCTAGTGGCAGCATAAAAAGACCAGCAGATGCCCCA







CAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene HPR. In some embodiments, the sequences from gene HPR comprise or consist of:











(SEQ ID NO: 758)



TGGGTCCACAGCTTTCCCTCCTGCCTTTCCTCTGGTTCTTTATTT







CAGTCTTTTTTGCATACATTGGTACAGATGCAGAAATAGAACAAA







GAAACAGGGCAAATGGGCTAAATTATAGTGAACCAAAGGGCTTAG







TGTGTTAAATCTTCTCCTTTTCTGCATCCATAGAAGACAGTGCTG







CTGTCTTTCCCAGGAGATAAGATTTACTCTCAGGAGTGTCTTTTT







CCTTGAGGTTACGTTTTTGTCTTTGTAGGGTATGTCATCAGCTCC







CGTGGTAGGCTTCCTGGCATTCGGAATATATTTACTAGCAGATAT







TTTCCTCTTTAAAAATGTATAATAAGAAAGACTAATAGTAACACA







TTTGAATGACACAATTAATTGACTAGTACTTGGGATACACACTAG







TACCTGGGATACATCTAATTAAGACACTTAGATCTTAAAAAATAA







AAAGACTTTTTGAAATGTTGAAATAATAAGACTAGAAACTTTTTT







TTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SERPINF2. In some embodiments, the sequences from gene SERPINF2 comprise or consist of:











(SEQ ID NO: 759)



TGTCCCCCAGGCTGGAGTGCAGTGGCTTGATCTTGGCTCATGGCA







ACCTCTGCCTCCCGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCC







GAGTTGCTGCGATTACAAGCGCCCGTCACCACAGAAGCCCAGCTC







ATTTTTGTATTTTTAGTAGAGATGAGGTTTTGCCATGTTGGTCAG







GCTGGTCTTGAACTCCTGACCTCAGGTGATCTGCCTGCCTCGGCC







TCCCAAAGTGCTAGGATTACAGGCGTGAGCCCCCATGCCCGGCCA







CACTGCTAGAAACTTTAAAAGCATCTCATTCGGTTACGTGTCCGC







CTGCAGTGGGACAGGTAGTATGACTCCACCCCACTGTGAGGAAGT







GGAGGAGCAGTGGGGTCAGTCACACGCCTGGCAGGATGGCCAGGA







GCCCCATTGTCTGCCTTAGGAGCACCTGCTGGCCCCACCCCCACT







TAGCTTCGGGGCTTTCTGTCCTCATGCTCTTCCCTTCCCTTTTCT







GTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SERPINF2. In some embodiments, the sequences from gene SERPINF2 comprise or consist of:











(SEQ ID NO: 760)



TAAGGAGGAGGGTGCGGGCGAGCCCCCGAGGTCAGGCTGGGCAGG







GCGGGTAAGGAGGAAGCGTCTGGCTGGCAGTGGAGCTGAGTCAGA







GCTGTCCTGTGTCCTGCTCTTTTCCAGGGCTTCGGGAGCGGGGAG







AGGGTTGAATATGAGCCCCCAGACCCTCTGTCCTAGAGGAAGTTG







GGGTGGCCCGGGGAATGGGGCTCTGCTTTGCTGCCCAAGACCTCG







CATTGCAAGGAGAGTCTGGGTAGAGAGCCTGGGATGGCATGTGAG







CCTCGCTGGCTTCCCTGGAAGGGGAAGGAGGAGAAGCTGGGGTGT







CACACTGTTAAAGAAATGCTGGGTGGAGTGGCTCATGCCTGTAAT







CCCAGCACGTTGGGAAGCTGAAGTGGGTGGATTGCTTGAGCCCGG







GAGTCCGACACCAGCCTGTGCAACACATTGAAACTCCGTCCCTAC







AAAAAATACAAAAATTAGCCAGGTGTGGTGCATGCTACTGGGGAG







GTTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SLC13A5. In some embodiments, the sequences from gene SLC13A5 comprise or consist of:











(SEQ ID NO: 761)



CTGTGCTGCTGACTTGTCTCTGGTTTTCTGCAGTGTGGGGAGCCC







AGGGAGGTGGATGAATGAACAGTTAGTTACGCCCTGCCCACCTGC







TGGGTGCCAGGCCTTCCTGTCCCTGTTGAATCCACTAGTTATCTC







GTTCGATCTTTGCAGCAACCCTGTGAGATAGGAAGGTGTTATTAT







CTTGCTTTGTCTTTCAAAAAAAAAGCGAGGCTCAGGGAGGCCAAG







GGAAGTGTCCAAAGTCACACATCAAGTTACTGGCAGTTACAGTTC







CAACCAAGAGCTTCCAACTCCATACCCCCTGCTCCTTCTGCTAGC







CATGAAGGGCTTTGGCCTTATAGGGCTTGTAGGGAAAGGTGAGTG







GCCAAGAGCAAGTCCATGCCAAGGGAAGATCTCCAAACATGAGTC







CCTGTCTGTTGCCTCCCCTGAGATAGGCACAGGACAAGTGATCAA







TGAGACAGGGTGGTCCTTGCCCTAAGAAGCAAAGTGTTTGGTTGG







GGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SLC13A5. In some embodiments, the sequences from gene SLC13A5 comprise or consist of:











(SEQ ID NO: 762)



CCATGGACTGCAAACTTGACTCCAATCCCCAGTAAAATATCTACA







ACAGGGTAGTGAAGCGATGGTTAGTGACCATGAGGGAAGCTTGCA







GAGCAGGCATCAGAAAGAGCCTGAGGAGGTCCACAGGGAAGCTGG







CACGTCCTTGTAGGATAGTTAAGGCACTGGGGTGAGCAATGAACC







TGGACTCACGGAACACTGGGCTCTGTGACCGTTTCCCTGAATGGC







CTAAGCTGTTGCCTCCTGTCACTTCTCTGAGGTCATTTTCCAAAT







GCGCACGGGCATAGAGAACCCATCCACTCTGCCTACTTCCCAGGG







ATGCCTTGAGCACTGAGGATACCTGGGGGACATGAAGTCGCACTG







TCCTGGGGGTCGGGACACCCCAGCCAGGGACAGAGCATGGCACAG







GGACATCGAGGCCCAGTGAGCCGACCCTTTGTCCTCCTCTCTGAG







AGCACTAGTCCCCAGCAGGCCTCAGGGTGCTGACTCTGTCTCTTT







TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ASGR2. In some embodiments, the sequences from gene ASGR2 comprise or consist of:











(SEQ ID NO: 763)



GTGAGGCCACGCTTCCCACAGGCAGGAGGAAGCCCTTGGAAGGCC







TGCTCTGGGAGGCTGGATCAGGCTTATTTTATCCATTTTTTTTTT







TTTTTTGAGATGGAGTCTCGCTCTGTCGCCCAAGCTGGAGTGCAG







TGGCACGATCTCGGCTCACTGCAAGCTCCGCCTTCTGGGTTCACG







CCATTCTCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCAC







GTGCCACCATGCCCGGCTAATTTTTTTGTATTTTTAGTAGAGACG







GGGTTTCACCGTCTTAGCCAGGCTGGTCTGGAACTCCTGACCTCA







TGATCCGCCCACCTCCGCCTCCCAAAGTGGTGGGATTACAGGCGT







GAACCACCGCGTCTGGCTGAACTTCAAAGTATTTTAATGCCACAG







TAGTTAAGCCATATGATACAGGGGCCAGAATATATGTATTGTTTG







CATTACATCAGGCTCTGTTAGTACTTTACAGCATACTGTGAGTAC







GAAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ASGR2. In some embodiments, the sequences from gene ASGR2 comprise or consist of:











(SEQ ID NO: 764)



TGTGTAGTGTGGTGTATATGTGTGTGTTGTGTGTGTATGCATGTG







TGGTGGTGTGTGTGAGATGAGTCTGTGTGTGTGCGGTATGGTATA







TGTGTGTGTACGCATGTGATGTGTGTGGTGTGTCTCTTGGTGTGT







TTGTGTGTGTGTGATGGATCTGTGTGTGGTGTGGTCTATGTGTGG







TGTACATGTCTGTGGTGTGTGTGTGGTATGGTGTGTGGTGTGGTC







TATGCATGGTGTACGTGTCTGTGGGTGTATATGCTTCCCCAGCCA







CAGGCTGGAGCCTAGCAGGTGGGGACCACACCTGCCGGCAGCACT







TTCAGCCTGTCTGTCCTAGATCACGATGCCCTGCTCTTCCATCTG







AAGCACTTCCCCGTGGACCTGCGCTTCGTGGCCTGCCAGATGGAG







CTCCTCCACAGCAACGGTAGGGGTGGGGCCGTCTCCAGGCCCAGT







GTGCCTGCCCCTCCGGCTGGATCTCATCTCCCTGCCCCCTGTCCC







CTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene TMEM220. In some embodiments, the sequences from gene TMEM220 comprise or consist of:











(SEQ ID NO: 765)



GTCAGGCGGCCGCGGTGGGAGGGGGACCCGCCCGGCTCCCAGCTC







CGCCCCCTGGCCTAGCGGGCCGTGTAACTGACTAGCGGGCAGAGT







CGCAGGGGCGCACAGTTAGTGGGGAGGACGGGACTTGGGCTTTCT







GCTTGGGGAGCCCCCACTTTCTCCCCCGTCTAAGAGACGCGGATG







TGTTCATGGAAGCGAGTCCCATACCAGGGCTCACACCCTGGAAGG







GGCTGGTTCCGCGCGTGGAACTTCCCAGGGGACCTCAGAAGAAGC







GGCCCTGGCTCCTGCGTTAACGTCCGGCCCGCGGACGACACTGAA







CATATCTCAGGGCCTAGGAGACGAAGCCGACCAAAACCCTTTATA







GCATCATTAAACTTTTTAGTCGGCTTGGGGACCCCCTTCATAATC







ATTTCCCTCATTTCTTGAGACTGGTGGGGGTGGAGGGCCCTTAAA







ATACCCGTTCTGGGTTGGCGAGTCCTCTCCAGAGCTCCCGTGAAG







GCTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene TMEM220. In some embodiments, the sequences from gene TMEM220 comprise or consist of:











(SEQ ID NO: 766)



TGAGCCGTATAAGATGTTAATATAACTGTCGTCACTCTAGAAATT







ACCATAATGCTTACAGGGATTTGAAAGTTCCTGAGCTTTGGAAGG







AAATGCATAAATACTAAACAGAACACACACACACACACACACACA







CACACACACACACACACACACATCTCTTTTTCTTTCTCTTTCTTA







TTTTTGTAAAAACAATTTTTATCCTTTTTTTTTTGAGGAGTCTCG







CTTTGTTGCCCAGGCTGGAGTGCAGTGGCACAATCTGGGCTCACT







GCAACCTCCGCCGCCCGGGTTCAAGCAATTCTCCTGCCTCAGCCT







CCTGAGTAGCTGGGATTACAGGCTCCCGCCACCACGCCCGACTAA







TTTTTTCGTATTTTTAGTAGAGATACGGGGTTTCACCATGTTGGC







CAGGCTGCTTTCGAACTCCTGACCTCAGGTGATCCGCCCACCTCG







GCCTCCCACAGTGCTGGGATTACAGGCGTGAGCCACTGCGCCCAG







CCCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene NCOR1. In some embodiments, the sequences from gene NCOR1 comprise or consist of:











(SEQ ID NO: 767)



TCTGAATTTTCAGAGTGAGAATTGTTGTCCTGTCCTTGAAGCAAA







TCACCTTCAAGTATTACAGTGTAATATTCACATTCTCTGGTAGAT







GAGAGACACAACTTTGTGTAGTGGATATTTCCAATCAGACGCTGG







CTAAGCCTTCACCTTGGCATTTGCCAGCTGTGTGGCCACAGGCAA







GTCACTTAACCTTTCATAGCTTTAGTTTTCTCATCTGTAAAATGA







AAATAATAGTACACGTCTACCTTGCTGTGGTTGTAAAGTTAAGGA







AGGAGCATTCGGTATTGTTATATAACAGGTGGCTCTGCATTATTT







TATGTATAATGTAGCACGTTATTACATATTAATTAGTGTATTAGT







ACTGTATATACACAGTAGATACTATGGAGGTACCTGTTTTCATTG







TTACATCACTGTTTTTATTTCAAGTGATGGATTTGAGTTGGTCCG







TTGCTGTTTTAGTTGGTGTGTGATACACTTTTACTTTCTTTAAAA







AAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene NCOR1. In some embodiments, the sequences from gene NCOR1 comprise or consist of:











(SEQ ID NO: 768)



GGCATGCCTCACTGCACCCAGCTAAATTTTTGTATTTTTGGTACA







AAGATACAAAATACCAGCCAGGCTGGTCTTGAACTCCCGACCTCA







GGTGATCTGCCTGCCTCGGCCTCCCAAAGTGATGGGATTACAGGC







ATGAGCCACTGCAATGCCTTAAGCATTATTCTTGAAGATAACTTT







GATTAAAAAATGATAAAATCAATTCAACCAATAACATGACAGAAA







TAAGCATTACATTACTTGTTAAGAGATGGCAATTATTACATTGGT







TGTTTGCATATTTCGATTTATTTTCTTCCTATAGGTATCCTGCAC







CCCATGTAACATACCAATTGGAACCCCAGTGAGCGGCTATGCTCT







CTACCAGCGACACATTAAAGCAATGCATGAGTCAGCACTCCTGGA







GGAGCAGCGGCAGAGACAAGAACAGATAGATTTGGAATGTAGAAG







TTCTACAAGTCCATGTGGCACATCCAAGAGTCCAAACAGAGAGTG







GGAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene UBB. In some embodiments, the sequences from gene UBB comprise or consist of:











(SEQ ID NO: 769)



TAAACTGCCTCTCGGGAGGTTGGGCGCGGCGAACTACTTTCAGCG







GCGCACGGAGACGGCGTCTACGTGAGGGGTGATAAGTGACGCAAC







ACTCGTTGCATAAATTTGCGCTCCGCCAGCCCGGAGCATTTAGGG







GCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGG







ACGTGGTTGGTGATTGGCAGGATCCTGGTATCCGCTAACAGGTAC







TGGCCCGCAGCCGTAACGACCTTGGGGGGGTGTGAGAGGGGGGAA







TGGGTGAGGTCAAGGTGGAGGCTTCTTGGGGTTGGGTGGGCCGCT







GAGGGGAGGGCGTGGGGGAGGGGAGGGCGAGGTGACGCGGCGCTG







GGCCTTTCCGGGACAGTGGGCCTTGTTGACCTGAGGGGGGCGAGG







GCGGTTGGCGCGCGCGCGCGTTGACGGAAACTAACGGACGCCTAA







CCGATCGGCGATTCTGTCGAGTTTACTTCGCGGGGAAGGCGGAAA







AGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene UBB. In some embodiments, the sequences from gene UBB comprise or consist of:











(SEQ ID NO: 770)



GTTTGTGTGGTTTCTGGAAGCCTTTACTTTGGAATCCCAGTGTGA







GAAAGGTGCCCCTTCTTGTGTTTCAATGGGATTTTTATTTCGCGA







GTCTTGTGGGTTTGGTTTTGTTTTCAGTTTGCCTAACACCGTGCT







TAGGTTTGAGGCAGATTGGAGTTCGGTCGGGGGAGTTTGAATATC







CGGAACAGTTAGTGGGGAAAGCTGTGGACGCTTGGTAAGAGAGCG







CTCTGGATTTTCCGCTGTTGACGTTGAAACCTTGAATGACGAATT







TCGTATTAAGTGACTTAGCCTTGTAAAATTGAGGGGAGGCTTGCG







GAATATTAACGTATTTAAGGCATTTTGAAGGAATAGTTGCTAATT







TTGAAGAATATTAGGTGTAAAAGCAAGAAATACAATGATCCTGAG







GTGACACGCTTATGTTTTACTTTTAAACTAGGTCAAAATGCAGAT







CTTCGTGAAAACCCTTACCGGCAAGACCATCACCCTTGAGGTGGA







GCCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene LRRC75A-AS1. In some embodiments, the sequences from gene LRRC75A-AS1 comprise or consist of:











(SEQ ID NO: 771)



CCTGAGGTCAGGAGTTCAAGACCAGCCTGGCTGGCCGACATGGTG







AAACCCCATCTCTACCAAAAATACAAAAATTAGCCGGGTGTGGTG







GCGTGTACTTGTAATCCCAGCTACTTTGGGGGCTGAGCCAGGAGA







ATCGCTTGAACCCGGGAGGCGGAGGTTGTAGTGAGTCGAAATCGT







GCCACTGCACTCCAGCCTGGGCAACAGCGAGACTCCATCTCAAAA







AAAAAAAAAAAATTACAACCTACTGAGAAGCTAATTAAGATTTGT







AGTCCTCAATAATTTACAAAAACTTAGAAAAATTACCTCTAATTA







GGTCTTAAGGGGCTTTTGTTAGGATGGACTTAAATTTGTAAAGTC







ATTAATTGACTGTCATCAGAAATGCCTGTCCTGCTCTGCATCTGC







AGTCTCTAAGGTGTATGCGCCTTCTCCATTCCCCCAGGTTTTGAG







AACAGAGTGCTGTTTTAGAGCTGGCAGCAGCATCTCAGCCCAAGA







GAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene LRRC75A-AS1. In some embodiments, the sequences from gene LRRC75A-AS1 comprise or consist of:











(SEQ ID NO: 772)



AAAAAAAGATATCAAATGATGAAATCACCCAAAATAGCTGGAATT







ACCGGCAGATTGTGTAGTGGTGAACCTATGGTTTTCTGAAGATAT







CTCTTACCCTGTGATTGACTGTCAGTTGGTGCAGAAAGTGAGATT







GAAATGTAGTTTCTTTGCAGGTTATATTCCCAGAGGATGTCAGTC







CCAAGGACCAGTAGCTGCCATCAGTTTGGATTCTGAAAACTAACT







GGCATCAACACTGGGTGTAGAAACATGCTTGCCTTATGTATCAGA







GGACATGCTCAGCAGATCCAAGAGATATATTTGGCAACTTTTTCT







AGAAAAGGCACATTGGGTATCATTCATTACATTCTTGAGTTTTTT







TGGGTTTTTTTTTTTTTTTTTGAGACAGTCTTGCTGTATTGCCCA







GGCTGGAGTGTGGTGGCACAATCACAGCTCATTGCATCCTCAATC







ACCCAGGCCTAAGCAATCCTCCCACCTTGTAGCTGGGACTACAGC







TCAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SKAP1. In some embodiments, the sequences from gene SKAP1 comprise or consist of:











(SEQ ID NO: 773)



GTAAGGACAGAAAGTGTGGGGCTTAAATAGAAAGTCAATTTTTTC







ATTCTTGATTTAGTCACCATAAACAGAATTATTCCATGTCATACA







CTTCCCATGGCTCCCTCACAATATAAATTCCCATTTATTCATAAT







TGTTAGGAAAAGTTTTAAATGAAAATCCAGATTCAGGAACCATTT







TCACATTTAAAAATACAAGGCTAGGCCGGGCGTGGTGGCTCACGC







CTGCAATCCTAGCACTTTGGGAGGCCGAGGCTGGTGAACTGCCTC







AGCTCAGGAGTTCAAGACCAGCCTGGGCAACATGCTGAAACCCAG







TCTCTACTAAACTACAGAAAATTAACCAGGTGTGGTAGCACATGC







CTGTAGTCCCAGCTACTTGGGAGGCTGAGGCATGAGAATTGCTTG







AACCCGGGAGGCGGAGCTTGCAGTGAGCTAAGATCACGCCACTGC







ACTCCAGCCTGGCGATGGAGCAAGACTCCATCTCAAAAATAAATA







AATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SKAP1. In some embodiments, the sequences from gene SKAP1 comprise or consist of:











(SEQ ID NO: 774)



CAAAGTTCCTATGGACAAAGAAGGCCGAAAAGTGAGCTGTGATCC







AGAAGCCTGCTGGCTGACTAAGCCTTTCTTGTAAATAGGCAGAAG







TCACAGATTTACTCTGATCCTTCAAATGCATAATGAAGAGAAAAA







CTAGATAGTTAGTTGCCCTGGATCAGGAAAGTCTGGCGGGAAACC







TCTGCACTGCTTACTTACAAACCAAGTGAAAAATTACCTGCCTGA







TTTTCTTTTATTAATAAATAAATATATTTATCATATTGTTGAACG







GCATCCTACTAGGAGTGATCTGTAAATATTTAATGTTAGTTAATG







ATCTCCTTCTCCCATAGTCCTTTGCTCATGCTGAGTGTTTGGCTG







TATCTCATCTCAACTCCCCTTAATCTGAGGTTTCCTAGGGGCAAT







ATCAGAACCCTGGTTTCCATTTGCAGCAAAAACATGGGTACCTCG







GAAAAAATAGAGTCCTTTTAACTTTGATTTCTCCTCTCTTTGATC







AGTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene TOM1L1. In some embodiments, the sequences from gene TOM1L1 comprise or consist of:











(SEQ ID NO: 775)



ATTCACCAACAGTTCAGTGATACTTGAATTCTGGTCTTCTTTCCC







AATCAGGCTGCTCCTATTATTCAGAGTCCTCAAATACTTGCTCTA







TGCATTCCGTCCACCTTTAAGTGACATTCAGTGGAGAGACAGAGT







GCAGTACGTTTGCTCCGCTTTATCCTGGACCGGAGACCTCACAGC







TTTCTTCTTAACCTGTGTTCTATACTACTTCTCATCCTTTAAGAA







ACGATGGGGTGGTAGCTACACTGATGGAAAGACTAAAGGCTCTGG







TCTTAGTGTTCCGTGCACACTCCACAGTCTCAGGGCTCCCACCAC







CCATGGCATCATAACCTGATGTGCACTAAAACCAATTGTTTTATA







AACAGTTATTACAAAATGACACTTTATGCAGTCTGAAGGTACCTT







TTAGGTTTTGTTTTTAGACTAGGAAAATTATCTTACTTTTTGCAT







TTGTGTTCTCACTGTAGGGTAATCATTCTGTGTTATCACACTATC







CTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene TOM1L1. In some embodiments, the sequences from gene TOM1L1 comprise or consist of:











(SEQ ID NO: 776)



TAAGTAACAAAACTCTTTTCTAGCAGTGCATCTAGTCAGCAGATT







ATTGTAGCCAGAAAAGAACTCATGGTCATTAATTCCCATGTTCTG







CTCAGTATTGTGTTGATGCGCAGCCCCTGTTAGCAAGATGGTAAG







GATCAAAATTTTTTTGGAGTTTGTATTTGCTATCTCCACTTCCTT







TCCTTGTATTCTCTTTGACTTCAGACATGCTCTTCTACCACTCTA







TGGACATAACTCTTGTCAAGGTCACTGGTGACTTCTGTATTGTGA







AATTTAGAACTGCAGTTCCATAGTCCCTCATTTTATCAGCAACAA







ATGACAAAGTTGATCACTTCCTCCTTCTTGCACACATGGCTTTCA







GACACCAGTCACTTTGGTTCTTCTCCTGCCTCACGTTCATTTCTT







CTCCATCTCTTTTGGTGGCTCTTCCTCCTCTACTGACCTTTTCTT







GTTGGAGACCCCAGGGATCAGGCTTCAGACTTTTCTATCTACATT







CATT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ABCA6. In some embodiments, the sequences from gene ABCA6 comprise or consist of:











(SEQ ID NO: 777)



GTAAGTTGATACATTTATTACCACTTTCTCTGCTTTAGATCTTAG







CTATATGTAGCTAATATTAATGCCCTAGTAGGTTACTGCAATCAC







TACTTTCATGTCCATTGTGTGTGTGCATGTGTGTGTCCTTGTGTG







TACTTGAAATAGTTTCTATGGAGTGAAAAAGTTATGTAATCCAAA







TAAAATTTGACTTGCCTTTTGCATAAAGGTCTGAACTAATGAAAT







GATGTATCAAAAAGAAAAAACAATGCTGACTCTAAAATATCAAAG







TCTTTTCTGTATAATTATTGAGGTTATTTTTGAGGGGTGGACCCA







ATTTTTTTTCCCTTAAGGGTGCTCATCACATGGTAGCTGATCACA







ATATCTCTTCTCCCTCTAATATTCTGTCTCATACTTACTTCTCGT







TTCACTCCGCTCTGCCTTCTCTGAACAAGAACCTCTAATTTCCAT







CTTCCAGGTTGTTTTCTGCAGAAAGTATGTAGATGCTCCTTCTTA







CATT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ABCA6. In some embodiments, the sequences from gene ABCA6 comprise or consist of:











(SEQ ID NO: 778)



TCTGCCTTCTAGGTTCAAGCGATTTTTCTGCCTCAGCCTCCCGAG







TAGCTGGGACTACAGGCATGCACCACCATGCCTGGCTAATTTTTT







TGCATTTTTAGTAGAGACGGGTTTCACCATGTTGGTCGGGCTGGT







CTGGAACTCTTGACCTCAAATGATCTGCCCACCTCAGCCTCCCAA







AGTGCTGAAATTACAGGCATGAGCCACCGTGCCTGGCCTTGGCAT







CCTTTCTATTGGTTGGTGCCTATACCAGTGTTCAGCATCTATCAT







CTCTGTATGCATATATGGTAAAGGTAACGAAGATGAATGGCAATT







ATAAAGAAGGAGAATTGCGATTGAAAAAGGATATGCAATGAGCTT







CTGCTGTAGGATTAACAGTATAGTTATTAATCTAGAAGGTACAAG







GGTAGTCATTACATTGTTCTTTAGATGCTTTTGTATATATTAAAT







ATTTTGTAAATAAGTTAACATAATTTATATAACTTTTTGTCTTTA







TTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene AFMID. In some embodiments, the sequences from gene AFMID comprise or consist of:











(SEQ ID NO: 779)



GCTTGGGAGGCTGAGGCAGGAGGATCGCTTGAGCCCAGGAGGTCG







AGGCTACAGTGATCCATGATCGCACCATTGCACTCCAGCCTGGGC







GACAGAGCGAGGCCCTATCGCTTAGAAAACAAAAAGTTACATGAA







GTTCCCCCAAGGTCCCGTAGCTAGCATGTGATGTGGCAGGGAGAG







AGCCTGTCCGCTTCACTCTCTCACATGGGACCAGAAAGGGGTGAT







GCTGTGCGTGGACAGGACATCTGGCAAGTGGTGTGTCCAGGACAC







AGGAAGCAGCGTAGTGGCCAAGCTGCCTCCAGCTGTCACATCTGT







GTGTCTCTGCAGTAAGGATGAGTCTGCCTTCATGGTCCACCCGCT







GACGGCACAGGGAGTGGCCGTGGTAATAGTGGCTTACGGCATCGC







CCCCAAAGGTAATAGGAGTGGTTGCTGCAGGTCCGAGGGCCGGTG







GGCTTTAGGAGGAAGTGCATCCCTGACCCAGCTCATGCTCTCTGT







GCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene AFMID. In some embodiments, the sequences from gene AFMID comprise or consist of:











(SEQ ID NO: 780)



TGGGTGTTGCCAGTAGATTTTCTTCCTGTTGGACCTCAGTGGGTG







GGAGGACAGTGCAATCAGTACCTAGGATACAGCCAAGCTGCCCCT







CTGGCCTGTGGAGCAGAAAGCAAATTGGGACTCTTCGAAGGGGGC







CTGATGTTGTGGGGAAACTGCGTGCAAATCCAGCTCTCTGCTCTG







ACCCCTCCCAGGGGAATTTACCTGTGTGGACACTCAGCCGGGGCC







CACCTGGCTGCCATGATGCTCCTGGCCGACTGGACCAAGCATGGG







GTCACGCCCAACCTCAGAGGTTTCCATGGGAGCTACAGCCTGGCT







GGGCAACCTTCATCTCCCCATGAGCCTTGGGGTTTGGGCCAACTG







CTTGAAATCCTCCCGGCCATGAGTGGCTTGACCGCAGGGCTTCGA







GCCCTCTGAGCAAGGCCTTCTCTGCCTCCTCTGTGCCCCTTCCCC







TGGTCCTGCCCCTCTGGCGGTGGGGGTGGGCTGGCCCTGCCTTGC







TCCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene TTC39C. In some embodiments, the sequences from gene TTC39C comprise or consist of:











(SEQ ID NO: 781)



AAGAACTGTTGATCCCAAAAGGGAAAAATTAGACATAGGGTGTGA







CGTGTCTAGATACCTCCACCTGCGCTCATGCACACATTCCTGCGC







CATAAAGGATTGATTTTTTTTTTTTAACCTTGGTGTTTTGTGTTT







GAGCTGTGTGCTAACAGTCAGTGTCAATGTCTGCCTTCTGAGCTG







TGGGCGATTCCCATTGTTGTGAGGTTTCCTCCAACTGGACGTTAA







GTTACTGAGAACAGGAATCAGGGATGCGATTGGTGCGTGTCTTCT







GTGGCTACCTGGTCAGCATTCATAAACGGTGATAATGGATTGAAT







GCTGCAGTTTCAACCTTCCTGACAACAGAGGAGAGTGTTACTTTG







GGGCATGAACAGTTATTGATCCAGCTGGGCCTTGGTTAATGAGTC







ATTTGGCTTACAGTGTTCCCGAGTCACAGGCTGTCCTGAAGATCA







CCTGCTGACTCATTGGTGCCCTTGTTTCCCCGAGGCCTCCTGCAG







TGAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene TTC39C. In some embodiments, the sequences from gene TTC39C comprise or consist of:











(SEQ ID NO: 782)



TATGTGACATTTAGCATTTCTCAATGCCTAAGATTAAACTGATGC







TTTGAATCCTGATCATGCTTTTGAGCTGCTCCTCTTGGGTACTTG







TGGCATCGTGTTGGCAGACCCTGAGTGACATCTTCAGCGGCGGGA







AAACGAAATGTATTTCACTGGGAGTAAGTAGAGCGGACATTTGAG







AGTTTGCTGGGTCCCAGACAGTGTTATGTGAATGAAAGTGTTGGA







AATGTAATCTGGATCCTCAGGGAATTTCTGAAGTGTCGGGAGAGG







AGATTTGATGAGCAGAAGCAATTGGAGAACAGCATTCTAGGGTAT







AGAAGCAAACTATCAACTGTATGGTGAAGAGCTCACTGAGAACTG







GGCTAATGGAGCGGCTTCGTGCATTCACTCAGTAGCGATTGAGAG







ACTTCTGGGCCAGGGCTGGGCGCACACGGGGATGAAATATGGTCC







CTTCCCTTTCCACACCTGTGTGGTAACAAGGGGCTAGATTAGCAA







ACAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MOCOS. In some embodiments, the sequences from gene MOCOS comprise or consist of:











(SEQ ID NO: 783)



GTTCAGTACTGAAGACCTAAACATTTGTCACAAGGGCAGTACCAT







CCACTATTTACACACATTTAAAATTATTAATGTGAGGTCCAGGTT







TGCCAGTTATAACAAACTTGTTTTCTTAAAAGTGAGTTAGTGGTG







CCAGGCGTGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGC







TGAGGCAGGTGGATTACTTGAGCCCAGGAGCTCAAGACAAGCCTG







GGCAACATGGTGAAACTCCATCTCTGCAAAAAATACAAAAATTAG







TCAGGCATGGTGGTGAATGCCTGTCGTCCCAGCTACTTGGGAGGC







TGAGGTGAGAGGATCACCTGAGCCCAGGAGGTTGAGGCTGCAGTG







AGCTGTGATCATACCACTGCACTCCAGCCTGGGTGACAGAGTGAG







TGAGACCGTGTCTCAAAAAAAAAAAAAAAAAAAAGAGTGAATTGA







GAATTTACATAAAGCTCATGATTCTCTTGTGTTTCATGATTGATT







TGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene MOCOS. In some embodiments, the sequences from gene MOCOS comprise or consist of:











(SEQ ID NO: 784)



TGGGTTTTCTTTCCATCCTGCTGACTTTATTACAACTCATCCTAGT







TCCAGACGAGAGCAATGTAGTGTGACAAAGTCCATGCACTGTTGA







AGAGGCCACCAGGCCCTCAGCCACATGCCTTTCCACCTTCATTCA







CCCTCGTTTTTCAGTCCTCTTTACTCAGCCAAAAGTGGTAACTTT







CAAATGGGAAGAAAGAGGCCAGTTCTTCTGATGGCAGGTGGGAAA







CTGTTGGGTCTTTGCCATAGTAGAATAAGCTGTTCACTTCACTTC







CTGGTCCACTAAAATGTCCAGAGTTGCACTGAGCACTCAGGCATG







GTGGTAGGTGGTTATCAGACGGGGTGTCACAGATGGACAACAGGA







ACCAAATGTTACTCCCAGAGCAGCACTGTGGGGAATGGATTCAAT







CGTTGGGTGTTTGGAAGACGGTGCTGGTTTTTCCTTGAAAGCACA







TCTGCATCCCCATTTTTATTTATTTTTTTGAGGTGGGGTCTCGCT







CTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PET100. In some embodiments, the sequences from gene PET100 comprise or consist of:











(SEQ ID NO: 785)



CTAATGTCTGCCTCCCATAAGCCGACCCTGCCCTGATGTGGGGCT







CTGGAGGCCTTTGGCTCGTGTCCCATTTGTGACTTTTCCTTACAG







AGGGAGCTGTGGCCACCTGAGAAGGTAAGTGATCTCTTCTTCCTG







CCAGAGGGATGGAGGAGGCTGGATTCTTGTATCCGGGATGGGTTT







TAGATGAAAATTTTAGGCCAGGCGCAGTGGCTCATGCTTGTAATC







CCAGGGCATGGGAGGCTGAGGTGGGTAGATCACTTGAGGTCAGGA







GTTCAAAACCAGCCTGGCCAACATGGCGAAACCCCATGTCTACAG







AAATACAAAACTTAGCCAGGCATGGTGGCAGGCGCCTATAATTCC







AGCTGCTTGGGAGGCTGAGGGAGAAGAATCACTTGAACCCAGGAG







GTGGAGGTTGCAGTGAGCCCAAGATCACGCCACTGCACTCCAGCC







TGGGCAATAGAGTGAGACTTTGTCTCAAAAAGAAACATTTTACTC







TCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PET100. In some embodiments, the sequences from gene PET100 comprise or consist of:











(SEQ ID NO: 786)



TAATTGGCAGGGGGCTTCCAGTGAAGACACTGAACCCTGAAGCCG







TGGTCTGGGGCAGGGTGGTGGGAGAGGTGGGCGGGGGGGGGTGGT







CCCGGCTCTGAGGTGTGTGCCCCTCCCCCCTTCCTGTCCCACCCG







CTTCTCCACCCCTAGCTTCAAGAGATAGAGGAATTCAAAGAGAGG







TTACGGAAGCGGCGGGAGGAGAAGCTCCTTCGCGACGCCCAGCAG







AACTCCTGAGGCCTCCAAGTGGGAGTCCTAGCCCCTCCCCTGATG







AAATATACATATACTCAGTTCCTTGTTATTCATTTAAGTGTTTTA







TTCTTTTATCAGTTTTTGGGGGCCGAGTGAGACCCAGGATGCCTC







AGGCCCTCAGGGGGCTTGTGTCGGGGGCTGGGGGCTGCTGTTCCG







ACGGAAGCCGAGAGCGGTCGGGTCCTGAGGGGTGAGCAGGGGTTG







GGGACTGAGGGTCCCAGCTCGTTTCTGTGCTCCGTCCTGTGGATG







AAGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PRKACA. In some embodiments, the sequences from gene PRKACA comprise or consist of:









(SEQ ID NO: 787)


GTGAGGCTGAGTCCTGTTACTATAGCAACTTCCTAGGCACACTCTGCCC





CTTAAGTCAGGGAGGGGCTGCAGCGGGGTCTGGGGCACAGGCGAAGAGA





AGAAGGATGTGAACCCCTGGCTGGCCCTGGAAACGCCTCCTGTACAGCC





CCGGGCTGTGCGGCCTGGGGAAGAGAGAGGGGATCGGTGGCCCAGTTCA





GACAAGGGAGGATGGTCCACCCGGCAGGGGGCCAGCTCAGAGAGGGGTG





GTGGAGTGGTCCATCCCAGTGTTGGGGTTCAGCTCAGACTGTAGGATGA





GCCATCCCAGTGTTGGGGTCCAGCTCAGAATGGGGGTCGTTCCAGCCAG





TGTTGGGGTCCAGCTCAGAGTTGTGTCATTTAGGTAAGCCCAGAGCAGG





GAATCCAGGCTCCACTGGAGGAATCAGCCAGCCTCGAGTAGGGGGCCCA





GCCCAGGCTGGGCATTCCTGCCCCAGAATTCCCAGGACAGCCAGATTTC





AAAAGGGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PRKACA. In some embodiments, the sequences from gene PRKACA comprise or consist of:









(SEQ ID NO: 788)


CAGCGGCCCCAGGGGGCAGGCGCCCAGGTCAGACAGCCAAGTGGGGCTC





AGGTCGTGGCTGGCACTGGCTGAAGATACCCCTGGGAGCTGGCCCACAG





GGGAAGGGGCAGAGCTAGTAAAAGGGAGCCCCCGATCCCCAGGACACAA





GCAGATCCTGGCAAAGAGAGAAAAGCACCCCCCAAGAAGACCCTAGATG





GGAAGAACTAGTGCCGTGTAACATCCTATGGATGCCTAAGACGGCCAAA





CACCTTCTGTGCCTTGGCAGTTGTATGGGTGCCCAAGAGGTCTTATCAA





GGCAGCTCTCCCCTTAGCGCAACCGCGTCTACAGAAACTCGGAGGGCCC





TATCTGTCCCTCCTCCACACCCAGTGGCCTCTGGGTTGGGTTTCTCTTC





CTGCTCCCACCCCACGGCTCCCTAGCTCCCCCTGCAGGCAGGGTTCTGG





GGACAGACAGCCGAACAGACACGGCAGGTCTCATGAGCCTTCCCAGCCA





CCGTAGTGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SLC27A5. In some embodiments, the sequences from gene SLC27A5 comprise or consist of:









(SEQ ID NO: 789)


GTCCGAGGCAGGGTTTCTCGGGGCGGGGCTGGCGCGGGGCCACGGACGC





TTTCAGATCCTAGAGGGAGTCAGGCTGAAGAGGCGGGACTTTCCTGTCG





TAAGAGGGCGGAACTTGGAGTCAGGACTTCTCACGCGAAGGCGGGGCTT





CTCGATTTTCGAGGGGGCGGGGCTGACTGGGCCTCCTGATCCCCAAAAG





GGGCGGGGCGGGGGCAAGAGGCGGGGCCTCTCCTTCCCGGGGGCGTGGT





CACAAGCGCCGGCTCCCTCCTTGCAGATGGAAGGGCGAGAACGTGTCCA





CGCACGAGGTGGAGGGCGTGTTGTCGCAGGTGGACTTCTTGCAACAGGT





TAACGTGTATGGCGTGTGCGTGCCAGGTGCGGAGGGTTCTCATTTTAAA





CTTCCAACAGCTTTGTGGGTGGAGGGGTCGTTATTCTGATTTTACAGGT





GGGGAAGTGCTAGGGCCCAGAGAGGTTTAGAGACTTGCTGGCTCTCACA





GCCAGTGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene SLC27A5. In some embodiments, the sequences from gene SLC27A5 comprise or consist of:









(SEQ ID NO: 790)


TTACCACCATCTGGGGCCTCCGCCTCAGCTTCTCGGCTCTGTCCAGCTC





CCCTGCCTTGATCTCTTGCTCTGATTTACTGGGTCTCTGGGTGTCCATC





TGATTTGTTCTCTTGAGCTTTCTGTGGAAGACGGCATTCCAGCCATTGG





TCCCTTGTCCTGTACCTTCTGTTAGTGTCTTCTCCCAGGGCCTTGCAAG





GTCCTGGTCATATCTATTCTCACTGCCATTTGTCTGTCCGTCTTCCATC





CCTTGTTATCCTTTTCCTGTTTCTCATGGGCTCCCCATACCGCCTTCTC





TATACTTCTCTCTCATTTTCTGTGTCGCTTGTCGCAGTCTCTCAAGGTC





TCTGAATGTCTCTCTCATCTCTGGATCTCCCTGTCTGTGTCTTTGGGTC





TGTCCTTGGCAGGGTGCTGGGTGATGAAGAGTCCACCGTTGTTGAAAAC





CTCCAGCAGGGGGCTTGGCTGGTGGGCTCCAGGGGTTCTTGTTCCTGCT





CCTACCCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ACSS2. In some embodiments, the sequences from gene ACSS2 comprise or consist of:









(SEQ ID NO: 791)


CACTTGATGAGGTCTGCACCTTCTCAGTGATGTCCACTCCCACAGTGTA





GTTGGCAGCTCCTCACTGGAGTTTGAGTCCTCTGGTAGGGTCCACGTCT





CCTTAGTAAAGTCTATATCCTCTGTATCTCTTAGGGGAAATCCATGTCT





CCTCACTAGGGTAAGTGCCCTTCAAAGCCATTCATGTCCTATTAGCAGG





GCCCAGGGCCCTTCAGTCAGATCTGAGAACCCCCAGCAACATCTGTGCT





CCCTTAGTGGAGTCCATGTACTGTCAGTGAAGTGTGTGTCCCTTCGGTG





CAGTGAATATCTCCTTTATGAAAGTATCTGACCCCTTGGTGGGGTCTAT





GTCCACCCCAAGAGGTCTGTACTCCTTCAGTGGGCCCTCAGATTCCCGC





CCTCCTTGGGATTGGGCAGACTCTCGGTCTCCCATAGAGAGATTTTTTT





TCAGCTTAGGGTGTGCAAGGTTTACAGTCAGCTGGGCAAAGTGCAGGGA





AGTAGCTGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene ACSS2. In some embodiments, the sequences from gene ACSS2 comprise or consist of:









(SEQ ID NO: 792)


TGAGAGATGGAGGAGGGTCCTGCCTGACTTCTTTCTGGGGTGGAGGGGC





TAGAGAGAAGCTAGAGGTAATTAAGCCCAGGTTGTGGTGGTGGTGGTGG





GGGTTTCCCTTGGTGGAGTAAAGAGATTGTGAAGGAAAGAAGAGAATGT





GGGCCAGGTGTGGTGGCTCACGCCTGTAATCCTAGCACTTTGGGAGCCC





AAGGCGGGCAGATCACCTGAGGTCAGGAGTTCAAGACCAGCCTGGACAA





CATGGTGAAATCCCATCTCTACTAAAAATACAAAAATTAGCTGGGTGTG





GTGGTGTGCACCTGTAATTCCAGCTACTCGGGAGGCTGAGGCAGGAGAA





TTGCTTGAATCCAGGAGGCAGAGGTTGCAGTCAGCCGAGATTGTGCCAT





TGCACTCCAGCCTGGGCGACAGAGCGAGAGACTCCATCTCAAAACAAAA





ACAACAACAAAAAAAGAGAATGTGAATGATTTTTCCTATTTGCACAGAC





TTTGATATT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene LBP. In some embodiments, the sequences from gene LBP comprise or consist of:









(SEQ ID NO: 793)


CCAAGGCAGAAAATCGCTTGAGCTCAGGAGTTCAAGACCAGCCTGGGCA





ACGTGGTAAAACCTGTCTCCACAAAAAAATACAAAAATTAGCCAGGCAT





GATGGCACATGCCTGTAGTCTCAGCTACTTCAGGGGCTGAGTTGGGAGG





ATGGCTTCAGGCCAGGCGACAGAGGTTGCAGTGAGCTGAGATTGTGCCA





CTGCACTCCAGCCTGGGTGACAAGGTGAGATCCTGTCCCCCACCCCCCC





GCAAAAGAAAAGAAAAATGTGGATTCTCAAGCCGCTTCCCCAAATCTGC





TGAATGTGATCATGCGATTGGGTGCCGCAGGCACTGGGAAGCCAGACAC





ACCGAATCAGGCTGGATGTGCTGGGACACAGCAGGGCGCCGGGAAGGGG





TGGAGAGGGCTCCCTTTGTGGACTGGCCTGACCAGGGACCAGGGCCTGG





CTTTCCCAGGCACCCGGCCATCCCCAAGTTCAGTGGCAGACTGTGCTTC





TCTCCCCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene LBP. In some embodiments, the sequences from gene LBP comprise or consist of:









(SEQ ID NO: 794)


TAAGAAGGTCCAAGCCTCTGGCCTCCCCCGAGCTTGGCGAGGGCTGAAT





GGAAGACCTCACTGACCAATGGCCCTGGACCAGGCCATGGGGGCTGGTT





TAGACGTACTTGGGCCCAAAGCCCAGGGAGGACAAATCATTTGCCCAGG





TCATGGAGCTGTTGTGGGGCACGCTGGGGACAAAGCTCCAAGTCCGTTC





GGTTGGGCAGAAAATGGTCAACTGCTTGACCAAGGGTTAAACAGCAGGG





GCTAAACAGAGACTCCAGCCCCTCCCTCTGCTCCACCTTGCTCAGTGCC





CCACACTGCCCTGCATTCCACCAAGGTCCGAAAGGAAACCATTCCTCAG





GCGCCTGCAAGAGACCAGGCAGGAAAGCTCAGCGAGGGCGTGGGCCAGG





TGGGAACTGGGCGAGCTCATGAGCACCAGCCCTGGATGGGGAGGCAGCA





ACACTGTCAGACTGGCCTGTTAAAAAGGAGTATGTGAAATCTTCAGAGT





GTATCATCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PABPC1L. In some embodiments, the sequences from gene PABPC1L comprise or consist of:









(SEQ ID NO: 795)


AATCCCTCCATGCCAAGGTAAGCAGGAGCCTGGGCAGCAGGGAGCCCGA





GGGGGCAGGAGGAGAGCTAACGACAAGGGCTCTCCTAGTGGAGCCAGTG





GATAGCTGTTTGGCCAGATAAAGTGTTTCTTCTTGCTAATACCTGAAGG





CCTGCTGAATCTTAAGAGTGGAAGGGGCCTTAGGTAAGGGTTTGCTACT





TCAGCCCTTCCGTAGACTAATTCTGTGTTGTTCTGTAGGTAGGTCTTTT





GGTACAAGGAAGTGTGTTGTGACTCAGTGTAAGGGTTAAGATTTTTCCA





GCTGTCCAGCAATGTACCTCTGTGTCAAAGGTGACAGACAGGGCCATGG





GATCTTGCAAGCAGTGGTGGATGGGAGGGTTCCACAGAAACCCTGTGGA





TGGAGGAGGATCCTGTATCCAGGATGGTGAGCGAGGGGGAGATCAAGGC





TCTTCTGTGACCTGCTAAGATAGCTGCACTAAGCCCCCCCGCCACTCAT





GTCTCACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene PABPC1L. In some embodiments, the sequences from gene PABPC1L comprise or consist of:









(SEQ ID NO: 796)


TGGGTGGAATGGTGACAGAAGCAGCTGAGCCCGGGAGAAGCTGTAAGGA





AATAGGCAGGGACTGACTTTCACAAACACTGAGAATTGCGCCGTGTTCC





TGGCTTTCTCTGAAGCACCAAAGAGTTTGGAGGAAGTTGTGAGAATCAG





AGGAGATGATTAACTGTGATCGGAATGGGGGGTGGTGGAGGGTGAAGGA





GGTGCCCTCTTGCCAGCTGCCCTGCCTGGCTTCATGCAGAATAGTTTGG





GCTTTCCCAGGGCCCTGCCTGGCTCCATTCTGCCTGCCTGAAGGCCTGG





CCAGGATGTAACCACCTTCTTTATTGAAGACTGGGTGTCTGCCATACTT





TAAAAACACTTATTTTTACCTTTTTGTCCTCAGAAAAGGAAATCCTCGC





TTCCATGGCTGCCAAAAGGACAGTGTTTCTGGCTCTCAGCCCTAAGGCC





CTGCAAACTCTAACTTATTTCCCAATTAGTCTGTATCTATACTTGGGCT





CTGTATGTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene UBE2V1. In some embodiments, the sequences from gene UBE2V1 comprise or consist of:









(SEQ ID NO: 797)


GTATGCTTGCAGGTTTTGCTGTTTCCTTTCAAATACCATATGTAGTACT





ATTTAATATTTTTCTTGTAATCAACTGACTCTTTTGCTTACATTTATTT





TAAAGGAAACTTTTACCATTATTATAAATGGGAAGTTAGTATATTACTT





ACCCTAAATAAGTTATTCTTAAACATAAATACGTGACTGTAAAACGAAG





TGTTCAATCACATACCACCGAAAGTCCTCTAACATACCACTACCAACTC





TGTCCTTCAGTTGCTTGGACCAAAAACCTGGGAGCTGTAGTTGACTCTT





GTCTCTCACACCTCACAGGCAACCTGATAGCAAATCTCCACAGCTCAGA





CCTCTGAAATGCCTAGAATTACGCCACTCCTCACTACTTTCCTGCTATC





ACCCTGGTCCAAACGGGTATTATCTTTTGCTAGGATTATTGCAGTAGCC





TCCTAGCTGCTTCTGCCCTTGCTCCCTTCACTCTCATCCTCACACAGCA





GTCTGAGGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the liver comprises or consists of sequences from the gene UBE2V1. In some embodiments, the sequences from gene UBE2V1 comprise or consist of:









(SEQ ID NO: 798)


AACAGCTGTTAACATCTTGTTCTGTATCCTTACCAACTCTATAAAGCTC





CTTAAGATGTCACTTATCATCTATATGAGTTTGCTAGTGCTGTCATAAC





AAAATACCATAGACTGGGTGGGGCTTAAACAACAAATTTCTCACAGCTC





TAGAGACCAGAAGCCCAAGATCAAGGTGTCAGCAGGTTTTGTCACATTC





TGAGGTACTGGGAGTTAGGACTTAAGTATATAAATTTGAGGGGAACACA





GTTCAGCCCAGCCCATAGCACGATCTGTGAACAAGTACCTCAGTCATCA





GCCTTCCTATCAATATGATTCTTTTGATGTTCATTTTTATTAGTGCAGG





CTTGTAAATTAACCAGTAGTAATGTAGTGACTTTTCCCCCGCTAGCCTA





ATTAGGATTATTAAAGATTTAGATTGAACCCTTTCTGAACAATAGTTGT





GTTGTCTGGCTTTTTGCCTATAATGTCACCACTATCTTTCAGTTTTATG





TTTGCTACA






Intronic domains that promote trans-splicing primarily in heart tissue


In some embodiments of the compositions of the disclosure, there may be an intronic domain that promotes trans-splicing primarily in heart tissue.


In some embodiments of the compositions of the disclosure, the intronic domain is derived or isolated from human genes that feature exons that are primarily included in heart tissue during the process of RNA splicing.


In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MASP2. In some embodiments, the sequences from gene MASP2 comprise or consist of:









(SEQ ID NO: 799)


GTGAGTTAAAACAAAGTACACCACGATCAATGACGATTGCTTTGAAATG





TAAACATATTTCTATCTGCAGATATGTGAAAGGAAAGTGAGAAAATCGT





GTGTCAGCATGATTTCTTTTGTCTATCTTTAAATGATAAAAAGCCAAGA





AATAAGTTTTCAGAGGACAAAAAGGATGGCTATGGAAAATGTGATTCTG





AATCCCGCCCTTTTTTCTTTCTTTCTTTATACTTAACTTTAAATTTACA





TAAGCGAGCCCTTTGTTTTAAAGCTAGTTTGGAAAGCTGGTGATTAAAA





TGGGCAACTTGGCCAGGCGCGGTGGCTCACACCTGTATTCCCAGCACTT





TGGGAGGCAGAGGCAGGCAGATCACTTGAGGCCAGGAGTTCAAGACCAG





CCTGGCCAACGTGGTGAAACCCCGTCTCCACTAAAAATACAAAAATTAG





ATGAGGTAGTAGCCCCGTCTCCACTAAAAATACAAAAATTATTAGGTGG





CGGGCGCCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MASP2. In some embodiments, the sequences from gene MASP2 comprise or consist of:









(SEQ ID NO: 800)


TTCACAACAGCCCTGGGGCAACTATTATCGTCAACCGCCCCCCATCTTG





CAGATGAAGAAACTGAGACTCAGAGAGGTTACACATAACCATGGGGAAA





TGGAGAGCTAGGATTTAAACACAGCTCCAGTCCCATGCAAGTCCCAAAG





CCTCCCCTGAGCTCTTTTCCTCGGAGTTACACTCTCTCTTGGTTTTGAG





CCATGGCTCTGAAGTTCCTGGCATGGAGCAGATGTTCTATTTGTGGGGA





CTGCACCATCTTCCCACATAACCCAGCTTTCTCACATACCTTTTGCTCA





GTCCCAGGGAAAACAAGAAACCTTAATTTGCAAAAGCACCTGAGAGTTT





AATCTTGCCTTTGGTTTTAAAGAACCCTACCCCACGTATGGTGAGCTCT





CTTTACATGTTAAGTATTCTTTATAAACTAAGAACTTCAGATGGCTCGA





TTCAGTCTTGGCTGCTGTAGACCACATGAAAACCAACAAGCCTGGTTTC





CTCTGCGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene AGMAT. In some embodiments, the sequences from gene AGMAT comprise or consist of:









(SEQ ID NO: 801)


GTAAGGAGATTCCACCAGTGAAGATCCTGGGGGTGGAGACACTTAACAC





CCATTTTCCTGGGACTTGAAGCGAATCTTTAGGAATCAGGATAGGATTT





TTGTTTGTTTGTTTGAGACGGAGTCTCACTCTGTCGCCCAGGCTGGAGT





GCAGTGGTGCGATCTCGGCTCACTGCAACCTCCGCCTCCCAGGTTCAAG





CGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGAGATTACAGGCACATGC





CACTACGCCCGGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACC





ATGTTGGTCAGGCTGGTCTTGAACTCCTGATCTCGTGATCCACTCACCT





CGGCTTCCCAAAGTGCTGGGATTACGGGTGTCAGCCACCGCGCCTGGCT





GCCAAGGTAGGATTTTAAGAGCAGAGCACCTAGGCCTTTTCTGTTCTCT





GGGCTGAGGATAAGTTTGAATACCCTCTTACTGAAGGGTGATTGATTTT





GGCAAGGGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene AGMAT. In some embodiments, the sequences from gene AGMAT comprise or consist of:









(SEQ ID NO: 802)


TGAGATCGCGCCACTGCACTCTAGCCTAGGTGACAAAGTGAGACTCCAT





CTCAAAAGAAAAAAAAAAGAGGGGAGGAAGTCCGGACCCAAGATGAGTT





GCCACCTTCTTAGTTCTGGTCCTCGTACCCTCTAGGCAGAATGGCTCTC





AGATAGACGTGTATGACAAGACCCCAATGGAACAGACCCAAATCCCTTG





CTTTTAGAGCGTGCCAAGGCCAGTCTTCTGGACAGAGCAATTCAAAAGG





TGCTAGCGTGAGCTGCTGTTTCCCACTCTTGCTTTAGCAAAGCTTCTCT





CTGATTCCAACTCCATCAGTCAGCTTTGACCCTGCTTCCTTACCACTCA





TTGTCCCTCCCTTCCTCACCTGTAAAATAGGATAGTTCCCTCTTCATGG





AGATGGAGGGTCAAGTGTCATGTGCACACAGAGCTGAGCATGGGGCTGG





CAAACAGGAAATGCCCTGTAAATGCTGCCAGACAGAAACCTCAGTCCCT





GTCTCTGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MFSD2A. In some embodiments, the sequences from gene MFSD2A comprise or consist of:









(SEQ ID NO: 803)


GCTTCCGCAATGAATTCCAGAATCTACTCCTGGCCATCATGGTGAGTGG





GACCTGAGCAGGGGCGGGCAGCCTGGGCTGAGGTGACATAGGCTGTGGA





ATGGTTCTTGGAATAGGCAGAGGATGTTTCTCAGGCTGGCCCAAGGTCA





TGAAAGGATGAGGGAGGCTTCTCAGGGTATTTGGTTGGACCTTGCGAAG





GTAGGACTGTGAAAGAAAGAGTTTGAGGTTAGATGTTTGAGTAGTGAGC





ACAGCAAAAGCAAGGGCGAGGACACCAGGAACTTGGCTCAGAGGTTTGA





GAGGGCAGCAGACAAGGTGGGCCTGGACCAGCTCTGAGCTCCCCTGGGG





AGCCGTCAAAGGTGCCTGTCATCTTCGTTGCTGCCCACATGATGTCATC





TGGCTGCTCTTGGGCAGGGCTGGGAGCCACTTTGGGGTTCTGGGAAGGG





CAGGAGGGGCTCTGTGGCTCTAAAGCACCTCCCTTTAACCCCCTTTGTC





CATCCACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MFSD2A. In some embodiments, the sequences from gene MFSD2A comprise or consist of:









(SEQ ID NO: 804)


TGAGTGGGGTTGAAGAGCAGAGCCTGGGTTGAGTTGGGATGTCTGGTGG





GAACCTCCCAGCTGATTCATCTTCCTGCACCCCCTTCCCTAGTCAGCAG





TGCCATTTCTCATCTTGGTGGCCCTCATGGAGAGTAACCTCATCATTAC





ATATGCGGTAGCTGTGGCAGCTGGCATCAGTGTGGCAGCTGCCTTCTTA





CTACCCTGGTAGGTATATACAGGCCCCCCTCCTCGGGTATCTCCAGCCC





CTAGTCCCCAGTTTTGAAGCTCCTTAGGGAGAGTTCTATGCAGTGTTCT





CCCACAGGCCATTCTGTGGGTCCAGGTTAGGAGTGGGGGAGGTCTGTCC





TGTACAGTTGTACAGGTAGTGAGCTTTGCAAGAACACCTAGTCAGAGTG





AAAAATGGGGGCTGTAATCTGGCCTGGGCTCCACTTGCCACGCTGAGCA





CCTCCAGGCAGGGTTACTTCCTCTTAGAGCAAGAGGCCTTTTCTTATTC





ATGTGAAGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene EBNA1BP2. In some embodiments, the sequences from gene EBNA1BP2 comprise or consist of:









(SEQ ID NO: 805)


GAAGCGGAAGTCCCAATCAAAAGTTGAGCAGTAGCTGTGAGTTGCTGGC





GCTGGAGCGATTCGGTGGCACGTGGAGCCACGGCGTGGGAGTAGGGGGC





TGAAGGCAGGCAGCAGCGGCCAGGGCCGCCCTCTGCTAGCCGCTTGGGT





CTCGGGATACCCCGTTTCTTCCTGTAGGTGTGGGACGTGCGTGCGGCGA





GATGGACACTCCCCCGCTCTCGGATTCGGAGTCGGAATCCGATGAATCC





CTTGTCACAGACAGAGAGGTAGGTGTCAGCCGAGCTCGTGGGGCTGGGC





GTGGGAGAGGGGGCCTTACACCCCTGGGAGTGACCGTGATTGTGTCCTG





CAGTTGCAGGATGCGTTTTCCCGAGGGCTTCTGAAGCCAGGCCTCAATG





TCGTGCTAGAGGGGCCGAAGAAGGCCGTGAACGACGTGGTGAGCGTGGG





CACAGGGTCGGGGGTACTCGGACATGGGATGCGCTTCAGGCGCTGTTGG





GCTTGGGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene EBNA1BP2. In some embodiments, the sequences from gene EBNA1BP2 comprise or consist of:









(SEQ ID NO: 806)


AGTTTCATAATACAAGTCGCCATTTATTTCCTCTACATTTATAATACTG





CATATATATTCAAATTGGCCAAAATCTTTCTTTCCAATCATCATGCTGG





GATTATGGGGTTATATGTGGCACATACCATTCCTGTATATGTCAGGATG





GAAAAAGTTTTACCTGGAATGAATTTTTGCCATTTCTGATCCACATTGT





ACTTCACACAGTGATTTCCTGAAGGAAATATAATGATCTGTTCATCGAA





GTAGAAGATATTGTTGGCCACGTGGGATCGAAGACCAAAAACATGCAGC





GTCTGAGCTACCACGGCTGACATGATCTCCCGCCAAACAGTTCTGCTGA





AGACCAAGGCAGCGCTTCAGAGTGGAGAGCACCGCCCCGCTCCGACCCC





GCCCCCGGCCCGGACTCGCCCTCCCCGCCCTTTGCTCCTCCCCTTGTCT





CTCCCCTTTCTCCTCCCCTTGTCCCTCCCCTTTCCCCTCCCCTTGTCCC





TCCCCTTTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PRDX1. In some embodiments, the sequences from gene PRDX1 comprise or consist of:









(SEQ ID NO: 807)


GTGAGTGAGACTCCGTCTCAAAAAAATGTGGAATAGAACAAACTTTAGT





TTTAGGAACTAGACTTTATATTGTAGATCTAGTTGCAATTGACACGTCC





TTGTTTTATGGGAGGCTACATACTTTAAAGGTTTCCAGGGGAGCAAATA





ATTAATTATGATTTTGTGTATGTGTGTTTGTGGGGGGTAGTCATCAGGA





AATGTCTGAGGCCTTTTGCCTTACCGTGTGGGTCTGGCTAATGTGTAAT





GTTTTGACTCAAGATTGTAAAGTCAAATTATTCATCCGGGGAGAGGATG





GTCGTGTAACTGAATCAGCCTCCCAAGATGAACTTTCCCTCTGGCGTAA





GTTTAGGAGTCCTGATATTTTATTTTCCTTTACAACAGAAAGCAGCAAC





CCTGTAATTAAAGAGAACAATGAAGGAAGCTATCTAAGCCATGAGGGTT





ACTGAGGATCTGCGCTGGGGTTAAGGCTGCGAACTTAAGAAATATTTGG





ATAGACAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PRDX1. In some embodiments, the sequences from gene PRDX1 comprise or consist of:









(SEQ ID NO: 808)


GCCTCCGGGTTCAAGCGATTCCCCTGCCTCAGCCTCCCAAGTAGCTAGG





ACTACAGGCGCGCCACCACACTCGGCTAAGTTTTTGTATTTTAGCAGAG





ACAGGGTTTCACCATGTTGGCCAGGATGGTCTCGATCTCCTGACCTTAT





GATCCGCCCTCCTAGGTGGCCTAAATCTACATTTTTACATAAGATAAGG





TGAATGTTTGAAGAAAAAGGGAATGGAGGAAGAAAACAATTATGTGGTG





GACTCAGGGTAATCTAGTAGTAAGGTACTACTAGAAAGGTAGAAAGTAG





TCTAGTAGAAAGGTAATCTAGTAGTGAACATTTATGGAATTTGTGGAAT





AGAGGCCAGGTGCGGTGGCTCGCGCTTACATTCCTAGCACTTTGGGAGG





CCAAGGCGGGCGGATCACCTGAGGTCAGGCGTTGGAGACCAGCCTAGCC





AACATGATGAAACCTCGTCTCTACTAAAAATACAAAAAATTAGCTGGGC





GTGGTGGTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene FGGY. In some embodiments, the sequences from gene FGGY comprise or consist of:









(SEQ ID NO: 809)


CTGCCCAAGACCATGGGAACCCACCTCTTGCATCAGTGTGACTTGGATA





TGAGACCTGGAGTCAAAGGAGGTCATTTTGGAGCTTTAAAATTTGATTG





CCTTGCTGGATTTTGGACTTGCCTGGGCCCTGAAACTCCTTTGTTTTGG





CCAATTTCTCCTGGAATGGCTGTATGTACCTAATACCTGTACCCGCATT





GTATCTAGGAAGTAACTGACTTGTTTTTTATTTTACAGGCTCATGGGTG





GAAGGGACTTGACTTGTCTCAGATGAGACTTTGGACTGTGGACTTTTGG





ATTAATTCTGAAATGAGTAAAGACTTTGGGGGACTTTTGGGAGGGCATG





ATTGGTTTTGAAATGTGAGGACCTGAGATTGGGAGGGGCCAGGGGCGGA





ATGATAATGATTTGGCTGTGTCTCTATTCAAATCTCAACTAACTTATAT





CTCCCAGAATTCCCACATGTTGTGGGAGGGACTTAGGGGGAGGGAATTG





AATCATAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene FGGY. In some embodiments, the sequences from gene FGGY comprise or consist of:









(SEQ ID NO: 810)


TAAGTCAAATTAAACATCCTATTCTTCCCAGTCTCAGGTTTGTCTTTAT





CAGCAGCATAAAAATGGACTAATGCAGTAAGTTAGTAACTTTCCCCCAT





ATTTTTGTTAGTGGAAAGATAGAATAGTTTTTTTTTGTTTTAATGGAAA





GATAGAATAAGAATCTTGGATTCTTTAAGGCCTGAACTTAGATGCCAGC





AGTGGCTGGGAAGGGCACAGGAAGGTGATAGTCCATGATGACGGGTTGA





TACAGACTTACTTAATATATGTTGACTATGTTTTATCCTTATTATGCCT





TCTTATGCCTTCTTATGCCTCTTTCTTTACCCTTCACCTTACAGCCAAT





CCAAGAGTTGGATATCTCAAAAAAATTAGGAGGCCTGGGTGCAAATGTT





TCCTTTCATTTCTACCTTTCCTATACCCCTATTAGGATGTGGAGGTGAA





CAGATGCCTGAAAATCTTGAAAAGGTTGTCCATGGGTCCAACTCTATAT





TAACTAAAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene LEPR. In some embodiments, the sequences from gene LEPR comprise or consist of:









(SEQ ID NO: 811)


TCTTTCTCCTTCCCCTTCTCTTCCCTCCCCTCCCATTATATAATCTATG





CTGTTATGTTACCTATTTTATCCACTACCCCATCTTTACTGGAGGTGTT





GTTCTAGTGGACTCTCCAGGAACTCCTCTATCCTCCTACTTCAATCAGG





ACTAGGCTTTCTCTAAAATTACTATAAGCCATCACTCTAAACTTTCCCT





TAACTGGTTTCTGGTTAGTACTGTTTCCTGGATTCCTTTCCTTCATCTT





TCTTAACTACACCCTTAAGTAACCTCCTAAGAAGTATGCGATAAGTCTC





CATCTCTCCATGCCTGAACTGCCTTTTATTCTGCCCTCCTCGATTGTTA





GTTTGGTGGAGTATCAATTCTTAGAAATAGCCTTTCCAAGAGAACATTG





AGATAATTCCTGCCTTAACATATAGTATCCAGTGTTGCTTCTGAGCAGT





CAGATACTATTGTGATTTTTACTCCTCACTGTTTACTTATAATTTCCTT





TTTAAACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene LEPR. In some embodiments, the sequences from gene LEPR comprise or consist of:









(SEQ ID NO: 812)


AAAAAAAAAGAAAGAAAAAAAAATTCTCCTCATTTTTGTTGTTTTGAAT





TTTCAGTGATGAATCTGGAGTGGTTCTTTTTTTATTTAATGTGGTTATT





TTTTGAAGAGACTTTCAATTCCATGTCTCCTGTCAATTCTAAGAAATTA





TATTATTTCTTTGATAATTTCCTCTCCTCTTGTTTCTTTGTTTCTCTTT





CTAGAATCTGTATGTTGGATGATGGACCCTCTGCGTAGATCTTCCACAT





CTCTTACCTTTTATCTCATATTTTCGGTTTCTTTGTTTTGTGTGTGTGT





GTGTGTGTGTTATATTCTGAGACCTTGCCATACCCTCTTCTTCCAGTGC





TTCTGTTGGATTTTTATATACTATTATTTTTGTAAATTTCCAAGTATTC





TTTCTCAGAGTATTCTGTTCTGGTTTTATGCATATTTTCTTAAATGTTG





CTAGAAATACCAATTCGAGGATTTTTGTTCTTTTATTTTAAAAATTCTT





TTTTCTTCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene GBP7. In some embodiments, the sequences from gene GBP7 comprise or consist of:









(SEQ ID NO: 813)


AAAGAAAAACTATGAGGTTCTTGCTTCAATTTATCATGGGCTTGAAAGA





ACAGTTTGACAGGGCACTGAGCTGGCAAGGGTTTGAGGATATTGTCAGT





GTCTCATATTTGCTAGTGAGAGTGTGAACTGTTGGAATCATATATGAAA





TATTTAAGTGCACATACCTTTGATCTAGCATTTCCACATTTAGAAATCT





ATCATATAGATTGATTTACTCAAATTGAAAATGGTATATATAAAATAGT





ACTTATGTTGCAGCATTGTTGTAATGCCAAAAGACTGAAAAAAACATTA





AAGCCCCTCTTTCAGGTACTGATAAGGGAAATCTTGGGAAACACTCATA





AAGGAATACTCTACAGCCACTAAAATGAAGGGAGAAGCTCTATTTGAAC





TAACAATGACAAGTCTCCAAGATGTATCATAAGTAAAAAAAAAAAATGA





TGCACGAAGCATGTATAATTACTACCACTGAATTTAGAAAATAATAAAT





GTATGCATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene GBP7. In some embodiments, the sequences from gene GBP7 comprise or consist of:









(SEQ ID NO: 814)


GAAAGTATCAGATTTGGCTTGATATGAAATGATACTGAATGATCTCAAA





TTGCTCCATTTGTTTCTGTATCTGCCTGACTCATCCTATACAACTAGAG





TAAAACATATTCCTTGTCCCAAACTGGAGCTTGACAACCAGTGTTTTAT





ACAGGGTCAGCAGTCCCCATTATTTGCTAGATGAGCATGGCCTTGCTAT





ATCGGTGGAAAGGGGAATCAATTAGAATTAAATCTGCATTCCCTCTCAA





AGGGCATTCATTGTGCTGGAACAAAATTCCTCCCCACCTCCAGTGAGAT





GGTGCTGAGGAAATGTCACAAAGGTGTATATCATGGATACTCCCCTTAT





TTAGGTCCAGTCATCTGGAAATGTCAGACCAACTCATCAAAGATTTTCT





AAGATGTTAGAGAAATGGACACATGTTTAACTAATGAAATTGTTAAATA





CAAACCTCGGTCTCTTAAAAATTGGGGATTTTCAAAAGTAGGCTCTTTT





ACTTTTATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ENSA. In some embodiments, the sequences from gene ENSA comprise or consist of:









(SEQ ID NO: 815)


GTGGGTGAAACATCCCTGTGGAGGATCCCAGTTATTGAGTCTTAGTCAC





AGATGATAATGTGATGCTGATTTGCATTTACCTTGCTGGAGGCAAGGCA





GGCGTGGGCAGGCTGTCACAGTCATGGAACATGTGTGGGAGCTGTGAGA





GTCAGAAGGAAAATACATTATCCTCATGAACAGGCCCAGTTTCTATTCC





TGGTCTGGGAGAATAATCATTTTGAGGTTGTTGGCTTGTGGTTGATCTC





CTGGTGGCTAGGATAGCCCCATATTTATCCTTCTCTATATCTTATTCAT





TTCTGTTTCTCCAGTTCCAGGCTATTGCCCTGTACCCAGTAAGGTCCTC





AGTTACCCTTTATGAATAAGTGATTTTGGTGGAACGATCCTCTACTGAG





TGGAGTACAAAGGATGGCAGGGGCTATCCCTTGACTCCAAAACTATATA





CTCTAGCTGGGAAATTGCTTATGTACCTGAAAAAGTCAGAACACACTGG





GGAGAAGAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ENSA. In some embodiments, the sequences from gene ENSA comprise or consist of:









(SEQ ID NO: 816)


GAAGTGAGCACCAGCCATTAATTATACTCCTGCAGCAAATGTATTTTTC





TTGTTCAGAGTGGTCCTCCTTACCAAGAGAGAGTCTACTTTCATAGCCA





TGTAGCTCCTGATCAGATTTTTAGTTCCCAAAGGTACCTAAAGCTGACT





GAGGGATTATATTATCGTTTTTTAAAAAAAAAATTTGGCCAGGCGCAGT





GGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGAGC





ACGAGGTCAGGAGATCGAGACCATAGTGAAACCCCGTCTCTATTAATAA





TACAAACACTTAGCCGGGTGTGGTGGCGGGCGCCTGTAGTCCCAGCTAC





TCGGGAGGCTGAAGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGCTTG





CAGTGAGCCGAGATCGTGCCACTGCACTCCAGCCTGGGCAACATAGCGA





GGCTCCATCTCAAAAAAAAATTTTTTTATTTTCCTTACTCTCCTCTGCA





ATGACTGTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SLC39A1. In some embodiments, the sequences from gene SLC39A1 comprise or consist of:









(SEQ ID NO: 817)


GTGGCCCACGAAGCTACCCAAGACATCTCTGCACAGCCCTAGCCTTTTT





GGCTTCACCCACTCCGTTCGGGAGTTGGGGACCCGGCCTCTACATTCCT





TAGGGGAACTCCAGCTCCAGGTCCTATTTGGAGGCGGCTCCTAGTTTCC





TAGTTTTCTGGGGGCACCAGGTGAAAGGGTGATGAGATATCCATACAGC





CTTTCTCATGCCTCGGTTTCCCCCGTGCAGTCTCCCTGAATAAGTCACT





TGGGAAAGAAAGTGTGAGAAGAGAGGAATGTTCCTGGTACAGGAGTAGA





TGAGTCACCCTGGGTAGGGGAAGGTCACAAAGTGGCTGGGGCCAGGGAG





CTGCACTCTGCTGACCAAGCTTCCCCATTCCTTGCCCCATCTTCCTTTG





CATGATTTTTCCCAACCACTCTGGTCTGTCTTCCTAGGTCTGAGAGTCA





CTGGAGCTACCAGAAGCATCATGGGGCCCTGGGGAGAGCCAGAGCTCCT





GGTGTGGCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SLC39A1. In some embodiments, the sequences from gene SLC39A1 comprise or consist of:









(SEQ ID NO: 818)


AGACTTGACAATGCGGGGTCTCCCTCTTTTAAAAATATCCATATAATCA





AAGCCTTTGTTGACTGGCCCGCTCCGGCCCCTCAGTCCTCCACAGATGC





GCCCCAGGGCCCCAGCCCCGAGGTCCTTTTCCTAATATCCGCTCTTGCT





CTCGCTCACTCTCCTCAGGTCGCCTGCTCCGCCCGCTCCCGCTCCCGAT





CTCTGATTGCTCCTAACTGACGTCACTCCCGGTCTGTCCCCGCCCACTC





GGTGCTGCCATTGGCAGTCGGTCGTGGGTACGCGGCTCCTGTGGGGGAG





GGGGGAGCTATGGGAGGGGGCGGGAATTCCCGACTCTAGGCCGGAAGCG





CGCGGAGACCATGTAGTGAGACCCTCGCGAGGTGAGTGCGGGCTGTCTG





GCAGCGGGGTCCATGTCGGCTGCCCAGGCCTCCCCGGGCCCGGGAACAG





GAGTCCCAGCTCACGCATTCTTCATCTGCTTCCCGCGACGCTTGGCGGC





CTCGCACCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene RAB13. In some embodiments, the sequences from gene RAB13 comprise or consist of:









(SEQ ID NO: 819)


GTAAGGCTCCTAAATTCTAGTCCCTCAACCCCTGGCTCTGACCCTTCCC





CCGTGCATGTCTCTGTATAGTAACCAAACCTCTCACCTCTCTGAGGCCT





TCAGGTAACTCTTCAACTGCTGCCAGCTCCTCATTTTCTTGGTACCATC





GCCTTAGTCTCCCCAAATCCACAAGAACTCTTCTCTATTTTTCTCTGTT





CCCCTCTTCCCCTACCCCCACCACCTAGAACTCTCTCGATCCTATCTCC





ATGTTTCCTGTATCCCTGTCCGCCACATCTTTTTCTCCCGATCCGATGC





TAGCCTCTTTCTGTCTTGCTGAGCTTCAAACTCTCCCACTGTGTCCCCA





CCTCACCATCTACTTCCCAGGTTCCCTGCCCCAGTCTCCCTGCACCTCA





GCTGAACAAGGGTTTGGGAAGGGTGGAACAAGGTGGCTGGGTGTGCTGG





AGGGTGGCCCAAGGACTGTGATCATGAATATGCAGCCAGCTTGGTGAGG





AACGTGGAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene RAB13. In some embodiments, the sequences from gene RAB13 comprise or consist of:









(SEQ ID NO: 820)


TCGTCGTGGTTAAACCTCTACTTCCCTTCACCCCAGCCTCTCTGGCCTC





AGAATGGAAGAGTAAGGTCCTAAGAGGGGAGCTACAATTACCGAAAGGC





TGTGGAAAATAGCGAGAGAGACAGGCCGGCCTAAGCTAGGAAGGGAAAA





CCGAGAGAACAGAGGCCGGGCTGGCAGCCTTGGCCTGCTCAAAAGGGTG





GGCGGGCAGGGGGTGGAGCCCGGAGGGGTGCCGCCTCTCCTCTTTCCCG





GAGCCTGGGCGGAGAGGGAGGAAAACTTCTTCCTGGCCTGGGCTCCGTG





CCGCTCTGTTTGCCAACCGTCCAGTCCCGCCTACCAGTGCCGGGCGCTC





CCCACCCCTCCCCCGGCTCCCCCGGTGTCCGCCATGGCCAAAGCCTACG





ACCACCTCTTCAAGTTGCTGCTGATCGGGGACTCGGGGGTGGGCAAGAC





TTGTCTGATCATTCGCTTTGCAGAGGACAACTTCAACAACACTTACATC





TCCACCATC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene FMO4. In some embodiments, the sequences from gene FMO4 comprise or consist of:









(SEQ ID NO: 821)


CTCCCAACGTGCTGGGGTTATAGGCATGAGCCACTATGACCAGCCTAAA





ATGGGGTCATTTTAAGTGTTTACATCTAACCAACTAATAGCTTTAACAC





AAACATGCACACACACACACACACACACACACGTACAATGTGGAGGGTA





TACATACAATATAATATAAATTATATTATTTATACAGCAAAAGCAGATG





AAGAGAGACAGGGCAGGCCAACTGGCACAGGTGCAGGTGTCTGGTGAGT





GTGGCGCAGGCTGACAGCTGGGCCATAGAGGCATCTCTCCTCACAATTC





TGGCAGTAGAGTACCCTGGTTAAGTGAACTGGGCGGGGAATTTTACCTC





CAGAAAATTATTTTCTGTGCTGCATTTCCATTCTGAGCTTTGACTATCA





TGTGCTCTGAAGTTAGAGCAGTGGGAGGCTAGAAATGAGTGGGTGGAGA





ATTTAAATAAAGGAAAAGCCACATGTGACTTTAGTGTTGTTTGTGATTT





TTCCCACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene FMO4. In some embodiments, the sequences from gene FMO4 comprise or consist of:









(SEQ ID NO: 822)


TATGAAATGTAGCTTGCTCTAGGGCAAACTTACAGTATATTCTGTTACA





AAAAAAGGATTCAGAGTATAAAAGCCATTCCTAGAGAAGCAGTATGGCA





GGTTGGAAATAACTCTGCATTAGGAATCAGAAGGATACATTTTTAGTCC





TAACTCTGCCACCAATCAGACGTGGCCTTAGACAAGTCATCTGGGGACA





CCAGGTCTTTTCAGTGGAACACACAGCCTGGCCCCCTACCAGTGGCAAT





CCTGTCTTCACTTAGTCCAGGAAATCTATTCTGACTTCACTCTCTTCCT





ACCTCTCAGGCTGCTCTTTCTCTGTATTTGCTGGTATCGCCTCTTCCTC





ACTCCCAAAATTTAGTGAGCTCCAAACTTAGTCCTCAGGCCTCTTCTCA





TTCCTATTTACACCCACTCCCAGGTGGCCTCATCCAGGCCTGTGGCTTT





AAATATCATCATATACTCAGGGCTCCCAAACTGTGTCTCTGACTCAAAT





CTTTCTCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CFHR3. In some embodiments, the sequences from gene CFHR3 comprise or consist of:









(SEQ ID NO: 823)


CAGATGACTATTAGAAAGATTATACCTTTCTACAGTTCTGTTTCTACAT





TGTGGGCATAAGAGAAAAATATAAACATAGAATTAAATTAGCAAAATGT





GAGTACATTTGGAGTGCTTGTAGTCACGGCTTGTCGAATGGGGGAAAGG





AGGATAAGTAACCATGACTCCAATGGAACATGTTAGCATAATCCTTTTT





GATATGAAGTCACTAGGGTGGACACAACATATTTTAGGAAAGAAGAGTT





CAGGCTCTAGAGAAAGACTGCCAGGGTTCACGTTTATCACCTCACCTGA





TGATCTCTGCTGTGATGTTGGGGAAGTGCAACTGAACTTATTATATTTT





TGAAATGCTAACGTCAGTATGTAGCACAAGTTAATAACTATTAACTATT





TGGATTATTTTATAATTTTATTTTATCCTAAACTACTCATTAGGATGCA





TTTTATTTGCTCATGAAAGAGAAAATTATGATTGTTAATTGTTTTTTTC





TGCTTTCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CFHR3. In some embodiments, the sequences from gene CFHR3 comprise or consist of:









(SEQ ID NO: 824)


AAAAAAGAAAAGAGGTTTTGCTAACCCTTTCAGAGCATTGGGAACACAG





CCAGAAGTGCATTAAATGTATATATTAACTTGGGCAATGTTGACACTTT





AGGATGCTGAAGCCAGGTGCAGTGGCACACGCCTGTAATCCTAGCACTT





TGAGAGGCCAAGCTGGCAATCATCAGAGGTCAAAAGTTCAAGACCAGCC





TGTTCAACACGGTGAAACCCTGTCTCTACTAAAAATAGAAAAACTAGCT





CGGCATGATGGCGTGCACCTGTAGTCCCAGCTACTCAGGAGGCTGAGGT





GGGAGAATCACTTTAACCAGTGGGGCAGAGGTTGAACTGAGCCAAGATA





GTGCCACTGCACTCCAGCCTGGGCAATAGAGTGAGACTCTGTCTTAAAA





ATAAATAAATAGGATGCTGAAAATTCCTATTTAAGGAAATAATTCTTTT





TCTGATTTATTTAAGGCTACATTTGTATTTTCTTAATACAAATATTTTG





AAAGTTTCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene C4BPB. In some embodiments, the sequences from gene C4BPB comprise or consist of:









(SEQ ID NO: 825)


TGACCTGACAGGAGGTGGAGCTCAGGCAGCAATGTGAGCAAAGCAGAGT





GGCTATAAATACAGCTGAAGCTTTGCTTGCCTGCCCGCTGCTCACTTTC





TGCTGTGTGGCCCAGTTCCTAATAGTCCGTGGACCGGTACCGGTCTGTG





GCCCTGGGGTTGGGGATCCCTGTTATAGAGCAAAGCACATAAGCACAAT





GCGTGAGAATTGGAAAAGTCTCTAAAATTCTTCTAGGCTCCAAAACTAA





ACAAACAACACTGAACAGATCCATTTTCATTGAGATTCTTCTTTTAGTT





CATATTTATTACTGGGATAATATTAATAAGTATATTTAAGCCAGAAAAT





ATAAATGATTTGCCATGTTGAAAACCCAAATCTTTACTTAAGCTAGCCT





GGTTCTGAGCCCCCATTCAGAGAGCTAACTGCTGCTCACTGGCTTCAGA





GTATATAGCTGGGTCTCAGTCCTTATGTTGTAACTTGTGAAAAATTTTC





TTTCTTCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene C4BPB. In some embodiments, the sequences from gene C4BPB comprise or consist of:









(SEQ ID NO: 826)


TTGGTTTCAGATTTACCTCTTATTGGGCTGAAATTGCCAACAGCAGTTG





TATAATGCACCATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT





GTATGTGTGTGTGTGTGTGTGTGTGTGTTGTTCTCAGAATGTGAAAAGG





CCAAATGTAGTGGTTGTCTAAGGCCAGAATTAGCAACATATTATTGGAG





AATAGAGGGCTTAATTTCCAGTCTGTAAGGAGAATGGAAAACTCTGATC





TCAGCCGAGAGAGTAACTCGTATTTTAACATGAGACCATGAATTATGAA





ACCAAAACTAACCGCAACTTATGAGGCAAAAATTAACCTTCCATTTTGG





AAAAGATGCTTAGAATAGTTACATCCTTAAAAATGACCCACTGGGACAG





AAGGAGAGCTAGGACCCAAATTCTCCTCCCAGTCTTGCCCACATCTGGC





TTACGGAAACAGGTTTCCAAAGCTGCAGGAAAATTGCTTACTGCCTCTT





GAATGAAAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene EPHX1. In some embodiments, the sequences from gene EPHX1 comprise or consist of:









(SEQ ID NO: 827)


AAGGCGGCAGCGGGGTTGGAAACCCACCCCCACACAGTCAACACCTGCT





GTCACACCAGGCTAATCAAGAACACTTTTCTTTGGTGCATTAACCACCT





GCTATTAAATAAAGGGCTGTTACTAGGTGGCAAAGTAACACACTGCAGT





AGAGCCTATTTGTAATCTGAGATCAGTGGCTGTAAGGCACCATCCTTGA





GCCAAGCAAGTACTAATATTCTTGTTATCTTAATTAGAATGCAAGAGAT





CGTGTTTTTAAAATTCTCTTTGAATCTGTCACTTTGCCCTCCTCTTCCT





GGGTGATACACCTGGTAGTGCTGGTGGGGGCCATCATAATGCCCCTTGT





CCCAGATTCCCTTCTTTTAGATGGGACTCGAGCACTGATCATTTCAGCC





CTGTATCTCTCAGGTCAGCGTGGTTCAGTTTGCTGTGCAGAGTCCAGGG





GAGATAACCACGCTGTGCACACATGAGATTGGCTGACTTGGCAGGACTG





TGCAATTGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene EPHX1. In some embodiments, the sequences from gene EPHX1 comprise or consist of:









(SEQ ID NO: 828)


TAAGGAGGGTGGTTTGTAAAGTTCACTGGGGAGGGTGATCCCTCTTTAT





TGTTCTAATATTATGCTCGCAGCCTCCTTGACAACATCATGCAAAATGT





GTCTTACTAGCTTCTAGTGCATAAAATATTGGTGGAGCTCTTCGCTGTG





CTGGGCCAGTCACCAGTGCTGGGCACTGTGGGTCAAGGCAGTGCCCAGG





ACGGGCACAGCCCTGCCTTCCCAAGCTATGGTTTATTGTGGTAGGATCT





GCCTGCCCAGTGAAGTGTGTTCAGCTGCTCCTGTTGCTGGGGGAAGCTC





TGCTCTCTGCCTTCGGCTGCCTCCCACACGCTCCCATGTGTCTCCCAGT





CCCTAACCCCTAGTCCTGCTTTCAATACCGCAGGGGCTTTTAGCTGACT





TTTCTCCATCTTCTTAAAGGGCAGGATTTCCTGGCAGAGACATAACGTC





TTTCTGGAAATGAAAGAAGCTGATGCATGTTTTTTCTTTTCTGAATTAC





TTAAATAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene RBM34. In some embodiments, the sequences from gene RBM34 comprise or consist of:









(SEQ ID NO: 829)


ATGAAAGCCTGCAATGTACTTTGGAGGGTGGATGGGTGACTTATTTACT





GTGTCAGCAGAATAGACTGAAAAGTTAATACTGATTATCATTGCTGGAA





TCCTAGGCATTCTCTTCCTATGTCCCATCATACACATTAGTATGGGAGG





TGGGTATGATTGCTGCAAACAGTCTTTGTTCTCTGAGCACAGACAGATA





AAGAATTTATGTCAATTTAATATTAAAACTTTATAGAAGTGAGTTGGAT





TTTACCAGCTCAGCACTCTGAACCAGTTATAGCCACATTCTTTGGAGCA





GGAAATAAGTATCATTTATATTTGGTTTTCCCATACATGATTTTTTTTT





TTAACTGCATAGGCATAGTTAGTTGACGTACCATAGTGTACATTTTTGG





GCACTATTACATAATTAATGTATATTAGAATATAGGAAATGTTTATTCT





ATTTTTTTTTTTGAGACAGAGTCTCGCTGTGTCTCACCCAGGCTGGAGT





GCAGTGGTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene RBM34. In some embodiments, the sequences from gene RBM34 comprise or consist of:









(SEQ ID NO: 830)


CCTACTTTCCTGAAATGTTCTTAAAAACTGGCAGTAGGCCGGGTGCGGT





GGCTCATGCCTGTAATCCCAGCACTTGAAGAGGCTGATGTGGTTGCATC





ACCTGAGGTCAGGAGTTTGAGACCAGACTGGCCAACATGGCGAAACCCC





GTCTCTACTAAAAATACGAAAATTAGCTGAGCATGGTGGCGGGCGCCTG





TAATCCCAGCTACTTGGGAGGCTGAGGTATGAGAATCACTTGAAGCCGT





GGGGGCGGAGGTTGCAGTGAGCTGAGATCACACCACTGCACTCCAGCCT





GGGCGAAAGTGCAAGACTCTGTCTCAAAAAAAAAAAAAAAAGCCTGGCA





GTAGAGGATTATCAGACAATTTTTCTTAATTCCTTTTTGTAGCACTTAT





TTGATGTTCTGAAGGTCAGCTAATGTGTATTTTTTTTTTTAATTTTAGA





AGCTGAAGTCGTTTTTTAAAGAGTATGGACAAATAGAATCTGTACGATT





TCGTTCTCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene KMO. In some embodiments, the sequences from gene KMO comprise or consist of:









(SEQ ID NO: 831)


TCATGAAATTTAAATGTAAGACCTTTTTGTCCAGTATAGATAAGTTCTG





AAATAAGGCAAAAGATAAAGTAAAAAATATATATATAAATATATAATGT





ATGTGCATACATTATATATGTGTGCATATACACACATGTACACATCTAG





ATATGTGCATGTGTGTGTACATATATACCAGGTATAATTATACCAGCTA





TGATTCAAGATTTGAGAACATAAGATGTTCTCTCTGTCTTTTTCCTTAC





ATATACACACACACGCACGTATTCTGAAATCTTGAAATATACCTGGTAT





CAAAAACCTACCTAAGGTTGAGTTTATAAAGACAGAACACAAAGTGTTT





TCTAAGACAACTTCTATTTTATGTAGCAATAGATGTGCGTATCATCGTG





TAGATGCTACTGGTTTTAATTACTGTGGAAACTATTATAAAGCTGAGTC





CGTTTTATAGGTTTGCTATATGAATGCACTAATCAATCATATATTCTTT





TTTCTGCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene KMO. In some embodiments, the sequences from gene KMO comprise or consist of:











(SEQ ID NO: 832)



TAGTATAGAATTTTTTATCAACTGTAATTTTGCATTTGTAATTAA







TGTTGTTTATTACTTACATTTGTACTTAGAATGTATCTACCTAAT







TGATCTCTTCATTGTTTAATCCATTTAGTGATTATAGCTTTGAAA







CTTTGGGATTACTGAGGAAAAATCTGAAGGATTTCCAATTATTTA







CTTTGGAAAGGAGATTTAACTTACATGAATGGATAACAAGAGTTT







ATGTGTGGTGGTGCCTGTCATCTCCACTAAAGACAATTTAGGAGG







AAACAGCCCTTCCAGTAAAGCCAAAGAGATTTAGGTTATCTATCA







GGAAGTGATTCAGCCTTTAAGGTCTGTAGTACATTGGAATACGCA







TTATCAAAAGAGAGCATGGGGATGGGCGCAGCGGCTCACACCTGT







AATACCAGCACTTTGGGAGGCCAAAGCGGGAGGATTGCTTGAGCT







CAGGAGTTCAAGACTGATGTGGCCAACATAGCAAGACCTTGCCTC







TACT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene RMDN2. In some embodiments, the sequences from gene RMDN2 comprise or consist of:











(SEQ ID NO: 833)



ACTTGTCCTAGGAATATCACCAGTTGTTTTTGTTCTTGAAAAGAA







AAGGATAGAGAAAGAAGACTGAGGAAAATGATTATGTAAAGAAAT







CTGAAAACCATAAAGTATCTCATTGCTTTGTAAGCTTTATGACCA







TGGTAATCAATTTTATTTTAATGGCTAAATGCAAAGGCTATGTTT







CCACCCAAAAGATACTTAAACTGATCAAGTGATTCAAATGTGAAA







CAACCAACCTAAATTCTTAGGGCAGTTTGGTTCATAATATTTGAT







TTATTTAAATATATTCTCTTCACTTGTGGTTACTGTTATTTTAAT







ATTGCCCTGTTATTCTCTCATGTTTTTCTCTCAAATCAGGCTGAA







GAACTATGCCCTGGTTATTCTAATCCCAATTACATGTACTTAGCA







AAGGTAATGAAGACCACTGTTCTGCTTTAAGATCACTTTGAACCT







ATCGCATAAAAACGGATTATGTTTAATATTGGTTATTTCTCATTT







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene RMDN2. In some embodiments, the sequences from gene RMDN2 comprise or consist of:











(SEQ ID NO: 834)



TAAGTCCAGAAAGTGACAGTGAGTGCTGTTGTCTTGTTAGTACTA







TTCGAGCTCAAAACAGGAATAACTCGCTTAGATACATTTGTAGTT







TTCTCTATTCAATTTTATGTATTTATTTTTATTCTACCTTTATTG







AAGTATAATTGACAAAAACTATATGTATACAGCATGATGTTTTGA







TAGACATATACATTATGAAATGACTACCACAATCAAGCTAATTAA







CACATTCATCGCCTCACATAGTTACTTTTTTTATTGTTGAGAACA







TTTGAGATCCACTCTCTTAGCAAATTTCAAATATACAATACAGTA







TTATTAACTACAGTCACATTGCTGTCCTTTAAATCTCCAGAACCT







ATTCATCCTGCATAACTGAAACTGTGTACCCTAGACCAATATCTC







CCCATTTCTCCCACTCTTCCAGCCCCTGACAGCCACCCTTCTACT







CTCTCCTTCTATGATGGAGTTTTCTTTCTTTTTTTTTTTATTATA







CTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SPTBN1. In some embodiments, the sequences from gene SPTBN1 comprise or consist of:











(SEQ ID NO: 835)



GCTCGCCGGGCCGCCGCCGCGTGGTTGGCGCCAGCGCACTGGGGC







AGTCGCGCGCGCCCAGGTGCGGGCCGCGTTACCTCAGCAGACGCT







AGAGAGTGAATGAGCCGCGCGGGCCCGGGACCCTTGGGGCTCTTC







ACTCTCCAGGCCGTCCCGTGGGCGCGCTAGCCTTTTCAAGTGATA







GTAATCGGAGACTTTTCCGTTACATGAGGCTCAACAAAAGATTGT







AATTCCAGCAGCTCTGTTTGGGAAGGCACTACCGCGGCGAGTCCA







GGGCCCGGCCGGGGGTCGGCGGCTGCCGGGCGGCTGGGGCGACCG







CGGACCGTGCGGGACCGGTAGGGGGTCGCGGGCCGGCTAGGCTCC







CCCGCGCCCCTCCTCGTGGTATAGCCTGCATTTCTAATACAATGC







TGTTATGCTAATCAGGTGACATCACCGCCCAGCACACGGCGAGTG







GCTCCTGATAAAATTACAAACCGGCGGCCGCCGCGGGCAGCTGGG







AGGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SPTBN1. In some embodiments, the sequences from gene SPTBN1 comprise or consist of:











(SEQ ID NO: 836)



TAAGCCCCCTCCCAAAGGCCGGGCCTGTCCTGGGTGCCAACGGGG







GTTCTTGGAGGCTTTATTTGTGACCCTAATGAAGACGGGCGGCTT







CACAGCTCGGCCCCAGACCCTGTGGTTGGCTGCGGGCACCCCATT







CACGACTTAGGGAAGGCAGTTGCTCATACTCCACTCCAGAAGAGC







TACCATTTGGAGTCCCTGTCTCTCCAGCACCTTTTTGGCTTTCTA







GGTTGTCAGATAACTGCTGCACACACATTTTCTTTACTTTTAGCT







AACTTTACCTCCTAGTAGATCAGTGCTCATAGACTTATTAGTATG







CAGGTATTTGATTCCGGGTCGCCTCTGGCTCCCAGCCCCCAGCCT







CTTACCTCTGGGAAGCTTTAAAGGTGTAAAGTGCCGTGCTCAGAC







CCAGTCCTGTAGAACAATAGCAAAGGGGAGGAGGGAGGGGTCTTT







TTGTTCCTAAGCCTTTGGTAAATTACAGTTTTTTATTCAGAAGCT







TAAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene NFU1. In some embodiments, the sequences from gene NFU1 comprise or consist of:











(SEQ ID NO: 837)



GTGGGTAGTGCATCTGATGGAGCCGGCGGGTCCGCCCTGTCGCCG







TAGTTGGGGGTGAGCCCTGAGGCTGTCTGTGCGGGAGCTCGGCGC







TTCTGTGGCCTAATCCGGGCGGACTCTCTGGAGCTTCCTCTCAGG







CTCAGAGACCCAGTTCTTGAGTCTCTGCGGTACGGAAGCTTGTAG







AGACTGGCTGTAGAGCCAGAACGGAGGAGCTGTTTTAAAATCGTG







TGCCTCTCCCGCCCCCAGGCCCTCTACCGGGTGCATTCCCTCCTC







TTCTAAACGCAGAATCAGAATGTCACACAGAAGGGAAGAAAATAC







CTTGTCTCGTTTCCTCATTTTCCCAGTAAGGCCCTGAAAGAGGAG







GTGACATGTCCAAGGTTACACAGTTTCTAAGTGATGGAATTCCGA







CGAGAACCAGGACCCCTAGATTCCCTGCACCCACCACCATTAAGC







CCACTCTGTTCCCTTGTTTTTTCCGCCTCCCTGAGCCCTACCTTT







GAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene NFU1. In some embodiments, the sequences from gene NFU1 comprise or consist of:











(SEQ ID NO: 838)



CGAGAACCTTTTAGAAGGAATGCTCCAGGGAGTCTTTGGGGGCAG







TCACTGAGCTGGGCCTGGAGGCTGAGTAGGGTCCTAGAGGAGGGA







ATGGGTCAGGTTTAGGGGCTGGAAACAGCATTCGCAAGGCAGCAC







CAGCACCAGCTAAAAACCTAGCCAACAGGGAAAACAAAGTACGGA







AACACGCATGCGTTCACAGTGCTGCGTGAATTAACCAAGCGTTGG







CGCAAACGGGAAAGGTTCCCCGGCCTCTCTTGGTCAGGGTGACGC







AGTAGCCTGCAAACCTCGGCGCGTAGGCCACCGCACTTATCCGCA







GCAGGACCGCCCGCAGCCGGTAGGGTGGGCTCTTCCCAGTGCCCG







CCCAGCTACCGGCCAGCCTGCGGCTGCGCAGATCTTTCGTGGTTC







TGTCAGGGAGACCCTTAGGCACTCCGGACTAAGATGGCGGCGACG







GCCAGGCGGGGCTGGGGAGCTGCGGCTGTTGCCGCCGGGCTGCGC







AGGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PCBP1-AS1. In some embodiments, the sequences from gene PCBP1-AS1 comprise or consist of:











(SEQ ID NO: 839)



GTTGGTTGAGGGGTTTAACAAGGCTAAGAAGGTGAGTCAGGTTTA







GACTTTGGAAGTTGAACCACAGGATTTATGCTACATACAGAGGAA







TGATTGTTGCTTCCTCTTATGTTAAGTTGGTATGTCACGTAAAAC







CTTTCAGAGTGCTTTCACGTTACTTGTTTAATCATCACAGACAAC







TTTGCCAGTAGGGTCTTATGCCCATTTTACAGAAGAGCCATTTTT







GGATTAGAGGAGTGAAGTGACTTGCCCAACGTTACACAGCCTGTT







AGAATTTGAGCTGAACCTCTTGACTCTTAGTTATGGATTACACCT







TTCCATGGCTTCTGATGCTTTTGATATGGACTTTGATGTGTGGGA







TAGCTTTACCTTAAACTCAAAAGGCTTTCTAGCCAGTTACTATTT







TTCTGTCCCCAAACAGCCAATGCATTTACCTTAAGTTCCTAACTC







TTGTTTTAGAAAATCTCTTCCCTCTTTTCCCTGATGTTTACATTT







ATGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PCBP1-AS1. In some embodiments, the sequences from gene PCBP1-AS1 comprise or consist of:











(SEQ ID NO: 840)



CTACCTACTGAAATATTTCCATAATTTCCTGTTATGTACTCCTGG







GACTTAGTGCACCTCCTCAGTGAAGCCTTCCCTTTCCCAGCTTCC







CCAGCTCTTAGCCTCCCTCCTTCCCCGAGAGTCTTAACAGTTTCT







CCCTTCGGTAGCCCACGTTTGGACGTTGGTTAAATTGTAATGTGT







CTTGGGGTTGGTAACATGTCATACAGACACCTTGGGTGATGCTGT







GGTCTGGCAAAGAGGTCATTGGACCTTGGAATCTGAGGCTCTGGG







CCCTAGTCCTAGCTCTGCCCCTGTCTAGCTCTGAGACTTCTACAA







CTCATCTGTCAGGGTTTCAGTTCTCCCTTTTATAAAGCAAGAGAT







TAAATGATCTCTAAGGGTCCTTCTACTTATGTCCAGGGACTGATG







ATATTTGATGACTTTGGTTATTTTTCTTTGGCGGGGTGGGAGGTT







TATGTAAATGTTTTCTGCACTTTTGGTAAAATCTCCACTCCCTTC







CTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene IL1R1. In some embodiments, the sequences from gene IL1R1 comprise or consist of:











(SEQ ID NO: 841)



TCCTTAAGTGAAAAATCTGTGAGGAGTTTTCTATCTTTCTAAGTC







TTTGTGGCACACTTATCACATGCATAGTACTATTTCTCAAGTTAC







CCAGGCACACACAGATGTATGGCTTTCAGGAAGCCATACATCTGT







GTAGCTCTGAGGCCCAGCCTTGTTCAACACTTCACTCAGAAGAAT







TGCTGGCCTGATGACCAACAATAATTGTCAACAACTTAAAACTTC







ATTGACGGCTCCTAAAAAAAATCAGTGTGCATGTGTGTGTGTATG







TGTGTGTGCGTGTGTGTGTGTGTGTGTGTATGAGTGTGTGTATAA







CATTCATTACTGCAAACTGGAATGGAGTGGGGCTGGAAGGCAGCC







CAGAAGCAAGGCTCAGAGCCTGGAGCTGCTGACCTCTTGGGGACA







CTGCAGCCCTGGCCTGGCCTACTTTTCTCTTCTCCCATATCTGGA







AAATGAAAGTAGCCTGACGTATCCGGGGACCCGGCCCAAGACTCC







ACCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene IL1R1. In some embodiments, the sequences from gene IL1R1 comprise or consist of:











(SEQ ID NO: 842)



TAAGGTTTTAAAGGGCCCGCCCTGGCTTCCTTGGGCCTGTAGTTT







GTGACTGTGTCGTTTTCAGAGTGGGAAGGCCCAGAGTTGGCCCCA







TGGAATTCTTTCTGCTTCTAGGGTGTTTGAAGAGATAGAAAGCCA







GGGTAAGGTTCACCGAACCTCCAAGCAAAAATAAATTTCCAGAAG







GGGCCCCTTTACTCTGAGGACATTGAATAGCAACAAAAACTACCA







CTGAAGAGTCATTTTGTCCCAGGCCCTGTGCTGGAAACTTTGAAC







ACCTGTGTTCACCCCTCCCTCATGACACTCATGAAGATATACCAT







GGCTGTCTTTATTTTCTAGGTGGGAATCTCAGGCTTGAGGGTTGG







GGTGGCTTGGGGAAGGTGTGTATCTAAGAGGTAGCTCTTATTTAT







TTTTACTTCTAGAGATTAAGACACATTCTTTTTCAAGATAGACAT







GGAACTGTCAAAAAGGATTAAAAGTTAAATAAGGATAAGGTTATG







TAAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene DBI. In some embodiments, the sequences from gene DBI comprise or consist of:











(SEQ ID NO: 843)



AGCGCGTGCACAGCCAGGCTGCGAAGGTGCAGCGGGCGGGAGGCC







CGTTGGGGGCTCAGCCGGCTGCCAGAAGCTCTCGGGCTCTTTCCT







TCCGTGCCCCTCACTTGCTCATGGGCCCATGCCTAGCCCTGATTC







GTTGGACAGAGCCTTGTGAGCGGGATTTTCCGTTTGGGGATTTCT







AAATCTGCTGCCCACCCCGCAACTGCCGGAAAGTTGCCCATGGGG







TGGACTTCGCTGTGTAGCGGGAGAGGGGTGGGAGTCGAGGGTGCT







TGATGGAGAGATGGGGGAAGGGGTTGCACGGATTGGAGGAGCGAG







GAGACTCAGTCCCCATCCCGAAGCACAGGGCAGGACGTCGCGGCG







GAGTGGGGAAGCGAGGAGTCCGTGGCCGAGAGCTTGGAGGTCAGG







GGAAGTACGGGGCCGGCTGCTCAGAGTGCGGGACGAGGAGAATCG







CGGCCCGGGGAGAGGTGACCCAGGGGCCCCTCCCTTCTCTCCAGT







GTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene DBI. In some embodiments, the sequences from gene DBI comprise or consist of:











(SEQ ID NO: 844)



TCGGTGTTGAACGCGCGGGCCCCAGGGGGAGGGAGGGGACCAACG







GGCTCCGGCGCTGACACCGCGGCACTCATGCCCTGTCCCCTTTCA







GCTGTTTCCAGCATACTGTGCCCCGTCTGTCCTCAGGCCAGGGCT







TCGCTGCAGCCCCGGCCACTCCCTAGTGCCTGGCCCGGTGGTGGC







CAGGCAGTTGGCCGCGCTGCTTCTCCCGCAGAGGGGACCCCCACT







GGGGGCGAAGGCTTGGCCTGCCCTCTTCACTGCTGTATTTCCAGA







CCTGATGCCTGCGTTTGTGAGAGCTCTGGATATATGGTTTTCGAT







TGAATGAGTGAACTGGAGGGGCTTCCCCTTCTTGTGTTGCTGAAT







CTTTCTAGCTGCCCTGTTGGGGCAGGGAGGGGCAGACACACTTCA







GGGGCTGCATTGCCCGAAGGGTGCCACCTTTCCCACCTCTCCATC







CCCGTAACTGGGCTGTCATCAGGCCACAGTAGGATTCTTACCCTC







TCCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene HNMT. In some embodiments, the sequences from gene HNMT comprise or consist of:











(SEQ ID NO: 845)



TCCCTAGAGTTGACTATTGCCTTGATTTTGGTTTCTTTTCTTAGT







TCTCCATGTACCTATTACTATTTTGATCCCCAAGCAGATATATTA







ATCTCCTCTCAATATATGCAAACATATCAAGGAATCTGTTTATTT







TACCCCAAGGGAACAACTTTTCTGAAGCCCTCTGTTCTTTTTTTC







AGTTTAGGAAGATCTGCTTTGGCCTGCTATACAACTATTAAGGAG







GGAATTTATTTCACTTTCTGAGAATCATTTTCTTTCTCCATTATT







TGATTCCCTGATTCTATATCTCATGCCTTTCTTTTCTTTGTTTCC







TCGGTCCTTTGTTTCCTGAACAATTCTTTAAGAACTTTAAGAGTG







AACGAGAGGTAAACTTTTTGAGACTTTGTATGTTTAAAAATCCCT







TGGTTCTATGTTAATCGTTTGACTGGATATAGAACTCTATACTGA







AAATGGTTTTGTATAATAGATCTAAAGGTACTTTTCCACTGTCTT







TTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene HNMT. In some embodiments, the sequences from gene HNMT comprise or consist of:











(SEQ ID NO: 846)



CCTTGGCATGTGGAATAACTTTTACATTGCTATGTGTTTTGTTTT







CCCTATTTTGAACTTTTTAGAATCTTCTTACGTCTAGTATTCTGA







AATTTTAAAATACTGAGCTGTGAAATGGTTCCTTTTTACCCATTT







TATGGGTACTCCCTGGGCTATTTCAATTGAGATTCAGATCCTTCA







GTTATGCGGAACTTTGGTAATCATTCTTCACCACTGTTTTCTTTT







TCTGGAACTTTTATTCATCAAATACTTCCTGGATTGATTACCTAA







TTTTCTTATATTTCCAGTTTGCTACAACTTTCCTCTTTCAGCCCA







TGTATTAACTTTTTACTATTATATTTTTAATTTCCAAGAATGTTT







TCTATAGCATTCTTTTCATCATTCCCAGATGTAATACCTTCTTAT







TCATTACAAAATGTTCCTCTGCTCTCTCTGCCTCTTTCCATCTCT







GTTTCTTATGTTAGACAGCTCCCCAACCACCTAAAGAGCCTTGGT







TTTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene KYNU. In some embodiments, the sequences from gene KYNU comprise or consist of:











(SEQ ID NO: 847)



GATCTGCCCACCTTGGCATCCCAAAGTGCCAGGATTACAGGCGTG







AGCCACACACCCGGCATGTTTTCTTTTTTTGGAAACAATAACAGG







ATATCAGATTGATAAATGCAATACCAATTTCTGATAATTTATCTT







GCTATATAATACTTCATGGACTTCTTGCACTTTGTGGTGTTTTAT







TAGTCAGGAATAAGGAGAAAACAACTTATAGCTGTGGAAAGAGTA







CAAATTAATTTTTAAAAACTGGAGAATAAATCAAGCAATTCGGCT







TTTGAAGACTTTCACTTGAAAAAACACTTTTTACTTTTTAATTAT







CATTTCTTAATAATAAAAATTATGCTCAAATGCTGCTCTTATGCC







AGATTTTTAAAGAACCTTAATCTGAAAAAAAAAACTATTTTATAA







TGCAAAAGGGCTCACGGTGAAATTTAGATCGGCAATATGAAATTA







CAAATTTATTATTATCGATATGACCTAACTTGTGCCTAACTTGAT







TTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene KYNU. In some embodiments, the sequences from gene KYNU comprise or consist of:











(SEQ ID NO: 848)



TATGCACGCCATTTACTTCTTCCCACCTTACTCCAAACATCACTC







TACTTAAGAGTGTTCTAATTGCATGACTTGTGAAGTACTTTAGCT







TGTGTCTGAGTAAAACTATTTCAAAAGTTAAAAAAAAAAAGAGTA







AACCTTGGGCCAGGCACGGTGGCTCACGCCTGTAATCCCAGCACT







TGGGAGGCTGGAGCAGGGGGATCACGAGGTCAGGAGTTTGAGACC







AGCCTGACAAAAATGGCGAAACCCCATCTCTACTGAAAATACAAA







AATTAGCTGGGCATGGTGGTGGGCACCTGTAATCCCAGCTATTCG







GGAGGCTGAGGCAGAAGAGTCACTTGAACTCGGGCGGTGGAGATT







GCAGTGAGCCAAGATCGTGCCCCTGCACTCCAGCCTGGGCAACAG







AGCAAGACTCCATCTCAAAAAAAAAAAAGCGAGTAAATCTTACAT







TTTTAAAAGAAAATCTGTAATCTTTTTTGCTTCAGGCATCTCTAT







TTAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene GRB14. In some embodiments, the sequences from gene GRB14 comprise or consist of:











(SEQ ID NO: 849)



GTAAGTTTGGTATTTTTAATTTTGAGTACTTGTGAATTGGATTTC







TCCTATAAGCAGAAATTATTTATTCCATAGCCACAAAGAGTTCTT







GATTTGGGCTTATCATAAAACAGAAGTGACATTGTTCTATAAATG







AGGTCATCACTGTATTCTAACAGTATCTATATTATACCTGGTACA







TAGTGTGTGTCTGGTATTTGTATATAAATGGACAAATAACGGAAT







AGAGTATCTTGTTCTTAACCAGTGATCACTAATATTACCCATTAT







GTCCTGATTCTAGCATCTCAGATATCAATTGATGGGGGAAAATGA







ACTTCTTTTTAAGTGTCAATATTTTGATTTGAACATATGATTTCG







AAGTATGGTTTATATTATGACATTATGTCAAATGTATTTTTTTAG







GTATGATGCAACATGTATTAAGTAAAAAGCTGTTTCTTAAGTGTT







TTGAACACTTATTTTTTTGTGTGTTTACATCCCTATTGTCCTTTC







TCTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene GRB14. In some embodiments, the sequences from gene GRB14 comprise or consist of:











(SEQ ID NO: 850)



AAAAACACTGAGAGAAAAACACTGAAATTGAAAAACACTTTCAAT







TTATTTGAAAGATAACTTTCAAAGAAATTTTCATAACTCAAATGT







GTTTCTTTTTTCCAGAGAAGTATATCAGAGAATTCCCTGGTAGCA







ATGGACTTCTCAGGCCAGAAAAGCAGAGTTATAGAAAATCCCACT







GAAGCCCTTTCAGTTGCGGTTGAAGAAGGACTCGCTTGGAGGGTA







CAAGTAGCTTCACATGCTTCAAAATATTTATGATTTCAGTTTGTT







TCACCTAAAAACTTGAGATTTGTTTTCTCCTTCCTTGCTCAGAAA







AAAGGATGTTTACGCCTGGGCACTCACGGTAGCCCCACTGCCTCT







TCACAGAGCTCTGCCACAAACATGGGTATGTCTGTTCATAGTCAT







TGCCACTGAGTAGACGGATAGACATGGAAAGGACATGTAATGAAA







TTGCAGTCATCTCAAACAAAGCTTTGCATTCATCCATCCTATTTC







TTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SPATS2L. In some embodiments, the sequences from gene SPATS2L comprise or consist of:











(SEQ ID NO: 851)



TCAGAAACAGTTATTGGTATTGCCAAAAAAAAAAAAAAAAAAGGA







AGCTAAGAAGTCACCCTTCTAAATAAATAAATAAATAAATAAATA







AATAAAAACCTGAATTGGAGAGCTGCCTGCATTTCACTCTACTGC







CTCTGGAAGAAGAGTGTTATCTAAAGTGCTTCCATCATGAATAAT







GATTGAGAAACTGCTTTTACTCTCCCGACTACTGTACTCCCAGGC







AGAAACCCACGGCTGAAGCCCACGATGCCCATGTGTAACTTGGTT







ATTACACACACGTTTGGAGCCCAGTGTGCAGACCCGCAGCGCAGA







GGAGGCGGCGGAACACCCCGGGTTGGTCGGGTTTCCAAGGGCTGA







CCCGAGCTCCAGCACTTTTTCCGCGCCTGATTTTTCAGGTCATTT







TCGAATGGGCTATCTTTGCTTACATTTTGAGCTTGGATCCTCTCC







AGAAAGCATTACTACCTTCTAAAAGGAAAAACATCTCGTATTTGC







AGAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SPATS2L. In some embodiments, the sequences from gene SPATS2L comprise or consist of:











(SEQ ID NO: 852)



TAGGAGAAGATGATTCTCTTGCAACACGTGCGGATTGTGACAAAA







TCTTTCATTAACAAGGGGAGTTTCGGTGAAGTGGAGGTTTGGGGA







AAGGCGAGGAAGTCGGTCTGGAGCAAGCAAGCAAAGTGCGGAAGC







TGTACTGGGATTCTTCTAGAAAGTGGGGTGGGAAAGGAGCTAGGG







AGGGCGTGTGGAGGGACGAGATCTGTGTCAGAACGTGCGTGTGAG







CGGATACAAAACCCGAGAGAGGCGTGAGCAGCGCTGTGTTTGCGA







GCGGGAGCGAGGGGCGCCGGCTGGGGTGTGTGCTCCTGGTGAGTG







ACACCGAGGGGAAGGCGGGCGGGTCGGCGGGCGGGGGCAGCCTGC







GAGGGGCGGGAGTGTCCCTGCGTGGGGCGGCCGAGCCGGAGTTGT







GTCAGTGAAGGAATCCAGTCCGGGGGCCGAGCTGGCTGCGCCCTC







CGCTGCAAGCGCCGGCAGCGCGGGGCGAGCTCCGGACGGCGCGCG







GCCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CPS1. In some embodiments, the sequences from gene CPS1 comprise or consist of:











(SEQ ID NO: 853)



AGGGCTGAGGATCGCTCCATCTTCTCAGCTGTCTTGGATGAGCTG







AAGGTGGCTCAGGCACCTTGGAAAGCTGTTAATACTTTGGTAAGG







AGAGAAACAAGTATCTGTTTCTAATGTTCTATTTTGAAGAGCTGC







AACCTGAATGTTGACTGAATGTTGACTATTGGATATGTTAACTAC







AGGGCAGATAGAATGCATACACTTAGTGAATTTAAAGTTGTTGTA







TATCCATGATTGCAGTTAATGCTGATTATGAGTTGATAGCAGTAT







TCTCATTAAGATTCAGACAATGAAAATAATTTCAAACCAGTTTTG







AACAAGATTTGATATTTAGAATCAGAAGCTAGGAATTTTATGTAT







TTCAGTGTCCAATTTACATTTAATGGTGCCTTTAGAGAATAAAGA







AGCTGGGCTACCACTCGAGCTAATGATACATTCTGTAATTTTATT







GCATATTTTAAACTACTCATACATTTTTATTGTTGTTTCTATTAA







TTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CPS1. In some embodiments, the sequences from gene CPS1 comprise or consist of:











(SEQ ID NO: 854)









TAACACTGTATATTGTTTTCCAAAACAATGATTCAAAAATTGGGA







GATACAGAAATGCATGCTGTATGCTAATAGCACTAGGGTTCTATA







TGTAGTAATTTATAAATCCATTAACAATTTCCAAACCATCACAGC







CAGTTTAAGGACCAATGTGTGGTCTTCTAGTTTAATGCTTTCCCT







TCAGGGAGTGGCCATAGGAAGACTACAGTTCTTCATCTAGAAGTG







TAGTGTAAGGCATCTCCTTACCAAACAGAGAGGGTAGCTTTATAT







TAAAGAACAGGACAATTTAAAAAAGACACACAAATTGAGGTATCT







CTCGCCAGCGTGTTTTCTGATACCCAGAAATGACAGCTTATCTTT







GACTATTGTCAGCACCTTCTGATAGTGCCTTTGTTTTAAAATTTT







CAGTTTTTGAGATCCGATTGTTTAATAGATAAATTATAAGGGTGA







GGAACAGGAACAGTGAGGATCTTTTATATTAAACTTTAAAACAAA







GTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PECR. In some embodiments, the sequences from gene PECR comprise or consist of:











(SEQ ID NO: 855)



GTAGGTAAACGATCTATATTGAAATTTATTGACAAATTATGCTTT







CTGATTCCATTTGTGATGTTGATAGAGGTATTTGGTAATAAGCAA







GTATAGCATAAAAGATTGACTTTAAAACATAGATAAAATGGAAAG







CCTAATTTTCTTTTTTTTTTTTTTTTTTTTTTGAGATGGAGTCTC







TGTCGCCCAGGCCGGAGTGCAGTGGCATGATCGTGGCTCACTATA







ACTTCCACCTCCAAGGCTCAAGTGATTCTTGTGCCTCAGCCTCCT







AAGTAGCTGGGACTACAGGCACAAGCCACCATGCCCAGCTAATTT







TTTGGATTTTAGTAGAGATGGGTTTTCACCATATTGCCCTGGGTG







GTCTCGAACTCCTGAGCTCAGGAGATCTGTTCGCCATGGCCTCTC







AAAGTGCTGGGATTACAGATGTGAGCCACCGCACCCGGCCCAGAA







GGTCTAATTATCATTATGGAAGTACTCTTTGATTTACAGCCAGAT







TGTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PECR. In some embodiments, the sequences from gene PECR comprise or consist of:











(SEQ ID NO: 856)



CCCGGTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGT







TGGCCAGGCTGGTCTTGAACTCTTGACCTCAGGTGATCCGCCCGC







CTTGGCCTCCCAAAGTGCTAGGATTATAGGTGTGAGCCACTGCAC







CTGGCCTCCTTTGTTATTTAAAATCGATGTTAAAAATAACCACAG







GCTTGAAATCCTTAAAATTACCAATGTGTTTTCCTTTGTAATCAA







AGTTCTTTAAAGTATTCCTCTTACGTAGTGATTTGGGGAGCCTTA







TCTGGGTCTATGCCATGTGAGATTTGCTTGATCATCAGCTGTGGC







TTGGATTTAATATTGAAGGACATAGTTTTTTCTTCGTTGTTTAGA







CAGAAGCTCAACAAGACAGACTCCATAGCAAGAAGTCTCTTGTGG







AATTCTGTCAGGTAGTTGGCACACTTGTGAGCTTGATGAACAAGA







GAAATATTCAACAGAAGCTGATTGTCATGTTTATGTTTTGTCTTT







CAAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SP100. In some embodiments, the sequences from gene SP100 comprise or consist of:











(SEQ ID NO: 857)



GTAGTTATTTTCAAATCTTTTCGAAAGGAAAGAAGGAAGGAAGGA







AAGAAAGACGCAGGAATATGTATAAAGGTATAGCAAACATCACAC







TTAATGGTGCTGCTAAGTGCTTCTTCCTTGAAATTGGAAATAAGG







CAAGGAAGTCCACTCTCATCACTTCTCTTCAACATTGTATTCAAA







CTCCTAACCAGTGAAATGAGACCAAAAAATAGAAATAATTGTTTG







GAAAGAAACAGTAAGTCAGTCTATTTGTTCATGATATAATTATTT







TCTTTAAAATACAAAGGAAATCTATAAATAATTTACTAGAAATAC







TACATGAATTTAGCAATGCCATAAATTCATGGTTAATATGTTTCA







AACCAATTATATTTTCATATAATAGTAATAAGGAATTTGAAAAGC







ATTATCATTTAAAGTAACACCAAAACAATAAAATAACTAGGAATA







AATTTATTATTTTTTAAATTTAATTAAATTAAATTTTTTATTTCA







ATAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SP100. In some embodiments, the sequences from gene SP100 comprise or consist of:











(SEQ ID NO: 858)



TAAGTAAATGGAGAGATATACAATGTTAACAGATTGGAAGCTCAA







TGTTGTTGTCATCTCCCCCAAATTTATCTGTAGCTTCAATTCAAA







TCCAAAAAAAAGTCCTAAAGTTTTTTCTTGAAGAAATTGACAAAC







TGATTTGAAAAGATAATGTATAAAAGCAAAGAATCTAGAAAATCC







AAGCCAATCTTTTCTAATTGACTTCAAGACTTTTGTTAAGCCATG







ATATTTAAGGCAAAATCTTACCAGCATAAGGGTAGACAAATAGAT







CAATGGTATAGCATAGAAAGTACATATATAAACCCATGCTAATAT







TGTAAATTGTTTTTTGAATATTTTCATGACCTTGGAGTAGGCAAA







TATTTCTTAGATAGTACACTAAAGTCATTAATTATAAAAGAAAAA







AAAATAAATTGGATTTCATCAAAATTACAGATTTCTTCCCATCAA







AAGACATGATTAAGAAAATGAAAGAGAAGCTACAGACTGGGTGAA







AATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene TRPM8. In some embodiments, the sequences from gene TRPM8 comprise or consist of:











(SEQ ID NO: 859)



CTTACTTTAAGGAAGGTCCCTGGAAGTTGTTCATTTCCTGCATAA







TAGTCCGTTGATTCTTGCATTTGCTGCACTTACGTTCTAGTTCAT







TAAAAGAATCTAGGCTGTTCCTTGGGGGACTCATCAAAAACCCAC







ATTTTTATGTTATTTCAGTTTTAATTCAGTTTTTAAAAATTTATG







CAGAAACCACTGAGCAAATAAAATGCTCAGAAACATTCTGGGTTT







TAACCTAGATTGAATGAGTTTGAATGAGTTGAGCCTTGATCATTC







AACCTTAGTCACGCAGCTCACACCGATAAACTGGTCAGAATACAC







ATCTTTAAGAGAAAAAAACATTCTCAGAGCTGCTTCTATAAAAAA







AAAAATCTCTCAACTTTGGTGGTATATTTGGATCTATTCCCAGTA







TATTCCCAGTATATTTTGAAGCAAACCATATCCTGCCTGAGAATG







GAAGTGGGAAATCTATTCTTAACACAGTGTTCTTTCTTTGTTTTT







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene TRPM8. In some embodiments, the sequences from gene TRPM8 comprise or consist of:











(SEQ ID NO: 860)



GTGTTGCTTTTGTCATTTTATTGAGAGTGACAAAATAAAATTCCT







TAATTCGGTCAGATTCACTTCTATAGGGATTTGGTTCCCAGAGTT







ACATGAACTGACTTCTCTAAGCAGATGACTAGAGCATTCTGATTA







AATGCAAGCAAATGAATTCCCCCCTCTGGTTCTGGTTCTTGTCAA







CATGGCCAGATCTGAATTCTGTGGCCCACTTTTTGGTTTCCCCCA







AGTCAGGCATCCATCTGTAGCCCCGGGGACTCCAGATTCATTGAC







TCAGAGCCACCCCAATGTGTCATGGGGCTTTTTTGAGGCCTCATG







TGGGCTCACAAACAGGTGCTCCTGCTCTATGTTGGGGTCAACACG







AATGTTGATTGTCCACAAACCACTTGACAATGGAGCTTCGGGCCC







AGCAAAGGACATGATTCCCTTGTTGTAGGCATTGTCTCCAGAGTG







TTCCTGGGCCCTCCTGCTCCAAACACACATTCATTCCAGGAAGAA







TCTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SEPT2. In some embodiments, the sequences from gene SEPT2 comprise or consist of:











(SEQ ID NO: 861)



AGTACATTCCTCACATTTGTGAACAGTTATGTTTCTAATTATATG







ATGTTCAAGTTCTGTAGTCTCATAGACCTGGATTCAAATCTTGGC







CCTGCTCTGGATAGTACTCTGACCTTGGGCAAATTAGAGTTTCTT







TGAGTACCCTCAAAGATCATACTGCAAACATAGTAGTACTGTAGG







TACTAAACACAGAATGATGTTTGTGAGGTACTTAGCCCAGTACCA







GGCCTTATGTAAACACTTAACCGATTGAATGGTGTCATATGAATA







AGGTCATGACAAGGTTTTTCTTTTTATTTAAAGGTGAATCAGGTC







TAGGAAAATCGACTCTCATAAACAGCCTATTCCTAACTGATCTGT







ACCCAGAAAGAGTCATACCTGGAGCAGCAGGTAAAAACATTCTTA







TGTTACTGTAAGTGTAATTCTACATGAAAGATGCTGAAGACTGCC







TAATTAATATTTTATGTCTTAGCTGTGTGTTTTGTTTGTTCTTGT







TCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SEPT2. In some embodiments, the sequences from gene SEPT2 comprise or consist of:











(SEQ ID NO: 862)



TAGGTGTGCTGCACCGAGGTCCTTGGATTTGGGTTGTAGACTTTC







TTTGACACGTATCCATGGATCCATCCTCTCTTGAGTTTGTCTGTA







AAATGTAATAAGTAGTTTCTGCCTATATTAAATATTTGATGGAAG







GATTAAGTGAGAATGTTTTACATCAAAAATGCTGTGTAACTAGAA







GATTACTAGGATAGATGTTCGAATTCATCTACTGTCAGAAAGTTC







CAGCACAGGACACCAGCATCCACAAATGACTGTTGAATGAAATAG







GCACACATAGTGAGAGCCTAAGACTTGGGCTGCTGCAAGAATATG







TAGCCCTTTCAGAATATTTTTGAATTCAGTTTTTAAATGAATTCG







TAAATTGCTTTCTTTTTCCCAGGAACAGAAATTATAATTTTTAAC







ATTTGTGTTTTTAACCTTCCCCAGTAGCACAATAATCTTAGCTTT







AACCTTCCCCTTTCTGACTTCTCTGGGATCTTTTTTTCTTAATCC







CCAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene RPL15. In some embodiments, the sequences from gene RPL15 comprise or consist of:











(SEQ ID NO: 863)



CAGAGACTCCGCAGGGGGAGAGCCCGCGGTGCGTGAAACAAACCT







GTTCTCTCCTCAGACCTCAAAGACAGCGGCTCCACCGCGGTACGC







GGCCACCGGCTTTGGAGCCTGGACCCCAACTTGCCTCCTCTCGCG







GAGAGACAGTCGCCGACGCTCGCTTAGCCGCCGAGACCTCGCCGC







CAACTCTCTCACCTCTCGAGACGCCCAGGCCGCTCAGGCTCGAAT







CTTGCGGAGCAGGGGGCGGGACAATAGCGGCCGCGGCGCCCCACT







CGGCAGAACTCCGCCACCAGGCGCGATGCCGGAACTACATGTCCC







ATGACGCTCTGGGAGGCCGCAGCTTTCCACCGGAAAGAGGGTGGC







TGAGGTGGGGGAGGAGCCCAAAAGGCATTGTGGGAGTACAGCTCT







TTCCTTTCCGTCTGGCGGCAGCCATCAGGTAGGCTGCGTTGAGGA







TCTTTGCTCTTCCATCCGCCTTTGATCGTCTTCCTCTTCAGCCAT







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene RPL15. In some embodiments, the sequences from gene RPL15 comprise or consist of:











(SEQ ID NO: 864)



TCAGGATGCGGGACAGCAAAGGGGTGGAGAGGCGGCCCCCGGGGC







GGGTTGAGCGAAGATGTGATGGCGGCGCGAATTCGAGCTGGGCCC







GGATCCTGGAGGAGGCGTGGGGCTGACGAATGGGGCGCCCGGGAC







AAGGCAGCCTAAAGTGAGGCTGCTGGTTGGGTGGGGTAGATGCAT







TTTCCTCGAGAGTTAATCCTCGGTGGCCGCAGCAGTACCTTGTCC







TGTGATGTCAGCGGCATCTCCTTTCAGGTCCTCCTTGGGGACGCC







GCCTGCCGCAGCCACCCGCCCCCTTGGTGCTCAGTTCTGGTTCTG







TTAATTCGCCCCACCAAAACGTGCCGAGCACCGCTCTGTGCTGGG







GTTGCGGAAGTGACTGAACGCGGCTCCGTGGGCGCAGTGGTGGGG







GTTGGGCTAGCTGTCCCCGGCAGTTGGTGCAGAGCCATTTTCATT







CCCGGCGGTTTCCTTGTTTTTGTTGGGGAACTAAGATGGACGGAT







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene AZ12. In some embodiments, the sequences from gene AZ12 comprise or consist of:











(SEQ ID NO: 865)



GTAACGAGCTCTTCTCTTAGGTTTTGGAGTCAGAGTTTGATTTGA







ATCCTACTCTACCCACTCTTCATTCAGTAATCAATATTTATTGAG







CAACTTCTACACAGTGATGAGTTGGGGACACAAATGATGGGCAAA







ACAGACAAAGCTTCTGCCCTCACAGAGCTTAACATTCTTCTAGAT







TTAACAGGGCTAATTCAACTTTCTAAGACCTACCTATAAAATATA







GATGATACATATTTCATAGAATTGTTCAGATAATTAAAAGAGATA







ATATTAAAAGAATGTAGTGCATTATAGTGGGACATATTGGACACT







CAAATGTTAATTAATTTGTCTTCTATTCTAAATATACAACAAACA







TAGCCAATTACTGGTATGGAAAGATAGAGTATGCATTAAGACTTC







TATAATATAATATTAATATGTTGTTGTAATGAATTATACTCAAAG







AACTTTTGAAAATTTATAAATTGAGAAGCTATGAAACTTGCTACT







AATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene AZ12. In some embodiments, the sequences from gene AZ12 comprise or consist of:











(SEQ ID NO: 866)



TATTCATGTGATCTGAAATAAGGCAAAAGAAATGTTAAAGGGATT







AGTTGACACAGGAGGGAGATTAGACATTTTGGAAGAAAGCAGGCA







TAGTGAAACACGGGTTGGAAACGTGGCTGTAGTTAAATTGCCTAT







GTTTACATCTCAACTCTCCACTTACTATGTTACTTTGGAAAAAAT







ACTTAAATTCCTCCTACCTCAGTTTCTTCATCTATAATGTTCTTG







AACAGTGACTGATATTAGTAAGTAACCAAATTTTATTTCAAGCAA







GGGATAGTTAAAAACTCACTTTCTAGTAATAATTACTGATCTAAG







ATACATGAAATTCAATTGATAATCTTTATTTAGAGTGCTATATTT







AGTTTTTTATTTTAAACTAAAAAATATTTAAAAGGACTTTTTAGG







TTCTCTTGCATGGTCTAAAGTCCCTCTTATTCTGTTTTTCCCAGG







AGGTCTTAAAAGGTTTTAGCCACAGTTTCTCCTTCCATCATGTTT







TTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene IP6K2. In some embodiments, the sequences from gene IP6K2 comprise or consist of:











(SEQ ID NO: 867)



GTAAGAATCCATTAAAGAAATTAATTTTAAATGTGCATTACCTGG







TTACATAAAGGACTCTAGAACAACTTAGGTTCTTTTGGGACTGTT







GAAACATTGTCTTTAAGGACAGAATGTACTGGGTGCCTTTCTCTG







TACTCTGAATATGCATGTGTATTGCGGTGGGGATGGGGGAGTTGG







GGGCCCTCACTTGGTCCTTCTCTGTTGTTTTTTTCCAGGACAAAG







CCAAAGGCCCCTTGTTAGCTGGCCATCCCTGCCCCATTTTTTCCC







CTGGTCCTTTCCCCTGTGGCCACAGGGAAGTGTGGCCTGAATACC







CCACCCCGGCTCCTCTGCACCCAGAGCTGGGGGCCACCTCAGAAG







TGTCATCTCTCTCTGAGCACGCATTCCCCTGCAGCAGTCGAGGAC







TGAGCAGATTGAGTGATGCTGGGGCAGAGAGGCCTGGGAGGAAAG







GTGTTCAGCCAGTCGTTTGTAAGGCGCTCGTCGGCACCTGCTGAA







ACGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene IP6K2. In some embodiments, the sequences from gene IP6K2 comprise or consist of:











(SEQ ID NO: 868)



AAGGCCCTGGCAGCCATCCCTGGAGCCCTCACTCCCTCCCACTCT







GTGCTTTCCCTCCCTCTCCTCCTTCTCCTCCTCCTGGCCCTCAGC







CCAGCATCTCACACCCTCAGTTTTCAATCCCTGGTAAGATTGCTC







AATTCTGTTTTGTTGTGTGGATTTGAGTTTGATTTTGGTAGAAGT







GTAGTTGTGTTTTATGTTCAGGTGTCTCTTGATCACAGTAACCCA







TAGTCCCCCACTGGGGGGACGGTGGGGGAAGACTTTGGGAGGATT







TTACCCAGAATACTTGCCGCCTGCTTTTTGTCCTCCAGGAAACCA







GAAGCCCGGGTAATTAGGTAGGCCGGGGAGCAGCGTTGAGTCAGA







TGGAATGCAGGAGTGGGAGGCCTGTGCTGATTAGCCCAAGCCCTT







TGCTGTTACAGAATGTCCCCAAAATTGCTATATGAAAGCAACCCT







TCGATTCACATATGATAATTTTTTATTTCTTTTTATAATGTATTT







TATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MST1. In some embodiments, the sequences from gene MST1 comprise or consist of:











(SEQ ID NO: 869)



GTGAGCACTAGTGACGCTTCCCCCATGACCCTGCCTCAGCCCCCA







CCCAAAGGCTGGCTCCCTTAACCCCAGTGAACTTTGTCTTTCAGC







TGATGACCAGCCGCCATCAATCCTGGACCCCCCAGGTTAGGAGTT







GGGCCAGTTATGGGTCAGGCCCTTTAGCCCACGACATCCACACAG







TCTGGGTTTCATCCAGCCCACCCCATCCTACAGACCAGGTGCAGT







TTGAGAAGTGTGGCAAGAGGGTGGATCGGCTGGATCAGCGGCGTT







CCAAGCTGCGCGTGGTTGGGGGCCATCCGGGCAACTCACCCTGGA







CAGTCAGCTTGCGGAATCGGTGAGGCACAACTGCCTGTCTCCCAC







AGAGAGGAGCTGAGGTTGTGTCCTCTGTGGTTATCCCACTGGGGG







CTGGGAATCTATCCCTGCCCCCAGAGGTCCTAGCCAGAAGATGGC







AGGTCTAGCATCTGTCCCAGGAGTCTGTTACCTGTCCTAATTCCC







CACT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MST1. In some embodiments, the sequences from gene MST1 comprise or consist of:











(SEQ ID NO: 870)



GGAGGCTGGCGGGCTAGGGCTGAGTGGAGCGCCTGCTTAGAGACC







TTCGGGAGAACTTCTGCCGGAACCCCGACGGCTCAGAGGCGCCCT







GGTGCTTCACACTGCGGCCCGGCATGCGCGCGGCCTTTTGCTACC







AGATCCGGCGTTGTACAGACGACGTGCGGCCCCAGGGTGAGGCCC







AAGCTTGGGGGCTACAGAGCCGGGCTGGAAGCCTGGAACCGGAGG







GCCGGGGCGAGGTCTCGGCCTGATGGCTGCCCGCACCCGCCACAG







ACTGCTACCACGGCGCAGGGGAGCAGTACCGCGGCACGGTCAGCA







AGACCCGCAAGGGTGTCCAGTGCCAGCGCTGGTCCGCTGAGACGC







CGCACAAGCCGCAGTGAGTCCCTGGTGCTCCCGGCCCCGCCAGGG







CCCTAACCCTGGGGCGGCATGCTTTGGTGTCTGGGACCAGAGCCT







GGAAATGGTTGAGACTACCCTGCCACGATTTTGCTCCCGCTTCCG







CCTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ITIH3. In some embodiments, the sequences from gene ITIH3 comprise or consist of:











(SEQ ID NO: 871)



ACATCCCATTGCCACCAGCCCCATAAAGGGCTTCCCTGGACGTGC







CCGATTTCCAAAGCCTAATCTTTGTAGTCACATCTCCACCTCTAT







CTGCCAGTGTTGGGGCCCCTCTGGGGCCCAGCAGCGCTAAGACAG







GAGGAAGGCACGGGGCTGGGGGAGAAGGAACCCCTAGACCCTGCT







CACCACTGCCCTTCGGCTTTTCAGCCACACCGGTGAGCCCCGCCA







TGTCCTACCTGAGTGAGTACATGCTGGCAGCTGCCACTCCTCCCA







CTGCTTAGAGCCTGCCCCTGCCACATGTCCCTCCACCGTGGCTAC







TCACTCAGCTCTCCGGGATGGGCAGATGGACAATGTGCCCTGAGG







AGATTGCGGGGTGGGAGGGCAATGGCATTAGCGAGCGGGAAGGGG







AGGGGAGGGGCATAAGCTGAACTCCTATGGGGGTCGTCGGGCGCC







TCCCAGGGCCACTTGCTTAGCTCAGCCCTCTCTCCCTTTGTATCT







GCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ITIH3. In some embodiments, the sequences from gene ITIH3 comprise or consist of:











(SEQ ID NO: 872)



TGAGTCCCTGGCTGCTCCTCGGGAAGGCTCAGGGGCCTGGGCACG







TGCTCCTGCCTGTCTCGGAGTCGAGCCCATGGAGGCCAGAAGGCT







GGGGGAGGCCCCAGGTATGATGAGAGGCCCCGGGAGTCAGCTCCT







TCTCTAATGTGTCACCAGTGGACGGGGATCCCCACTTCATCATCC







AAATTCCGGAGAAAGACGATGCCCTCTGCTTCAACATCGATGAAG







CCCCAGGCACAGTGCTGCGCCTTATTCAGGATGCAGTCACAGGTG







AGGCTTGTGGGCTAGGGCCGGGGCCAGGGGGCTCTTCCTGGGAGG







GCTTGGGTCCCCCGAGGTAGGCACAGGGAACAGGTTCTCACTCTG







CCCAGTAAGGCATCATCTTCACATGCAAAGAAACCCCTGGGGGTG







GGGAGTGCCCAGGGCCACAGGCTTGCTCAGCCCCACATGCCAGTG







CTATTGGGAGATGATTAGCGTGAATGGACCACCTGGGCCTAGGGT







AGGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ITIH4. In some embodiments, the sequences from gene ITIH4 comprise or consist of:











(SEQ ID NO: 873)



GTGACAAAGTAGGAGGTGGGGGGAGGGGAGATTGACAGATTGGGC







TCAGTCCTGGCTGTGGAGGGTAGTGCCTTGCCCAGCTCCTCTGGC







TGGTGACAGCCCTCGGGCCTCAGTTCAGTTGTATCTGATCAGGAC







TTGACCACACAGAGGCTTCCTTTTCTCCAAGAAGAGGATGGAATA







GACAAGGTAGTTTGAGGGTTTCTGCCAGGGGGAGGCATGACATCC







CTGGGGGGGAGCTTGAGGGAGGTCCATTATTGTTCATCTCCCAGC







TGTTGGGGGAGAGGCTCCCCAAGTTGGCCGGGTGTGGGGAGAGGG







GACATAGCTGCCCTCTCCGCACCCACTCCTGGGTGGATCTCAAGC







CCTGGCTGTGAAGACCCACACCCCACCTGAGAGAAGACTGCATCT







GTCGATTTCTCCAAAGCTGGAGCTGCTGGCTCCCGGATGAATTTC







AGACCTGGGGTTCTCAGCTCCAGGCAACTTGGACTCCCAGGACCT







CCTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ITIH4. In some embodiments, the sequences from gene ITIH4 comprise or consist of:











(SEQ ID NO: 874)



TGAGGAAAGTGAAGCCGGGTCTCCTGTCTCCCCAAATTCATTGCA







CCTACGATATAGTCCAGGATTGGGTAGCAAGATTAAACACAAGCG







CTTCAAATTGATGAAAACCACCCTGACGGTGTCTGTCTGTGTCTC







TGTCTGTCTGTCTCTCTCTCTCTCTTTGTTTTATCCAGAAAGTAG







AAACAGGAATGTCCACTCAGGTAAGCAGCGAACATCCTCAAAGGC







TGTGCTGGGTGGTGGTCTGCTAACACAGGGTCCAGGGCACCAGCC







CTGTGAGGTTGCTGGCCAGGGCCCAGGTCCACCCAGGCCTCTCCA







GCCCATCCCTGCTGCCAGGAGGCAGTGTAATCTGACCCAGCATGC







TGGTGCATTTAGTCGCCTGTGCATTCAAATATTGGCTGTGCCTCA







GGCCCTGTCCCCAGCACTAAGAATACAGTCCCCATCCAAGTGGGG







CCACAGGAAGGTGGGTGCAAGATGCAAACCCAGGTCCCACTGATT







TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene IL17RB. In some embodiments, the sequences from gene IL17RB comprise or consist of:











(SEQ ID NO: 875)



TTTGTGTTAGAAGGGGAACATTTTAAAGAACCTGAGGAAAGTACC







ATGGACTGCATTCAGAGCTTTCTTTTGTTTATCTCCTTAGCATAG







CTACTCCTTAACCTGGGGTAGCCCAGTAAATACTCTAGGCCGTCC







TAAAAACCTAGAGTTAAACATTAGAGTGTGAAGGGGCCTCACATC







GGTCCCAAGTCATCCCCTGCTTGCAGCAGGGAAGGATGACATCCA







GAGACAGATGGAGACCCACCTGTGGTCTTCAAGTGAGGTTATGCA







CTGGCTTGAGGATCAGGTATACTGATGGCTAATCCATGGTCCGTC







ATAGCCTACAGTGGGACAGCTTAGTTCCCCCCTAAGCTGTGAAGC







AGAACCAGTTCATCTTTTCTTTGAAAGTTTGTCACTTGTATATGC







ATTTAAGGGGAAAATGGTGTTCTGCCTAATTTGGCAAAGGAAAGC







TTGTGGTTTGCCGGCTTCAACGTGACGCTTGGTTTTTTCTCTCCC







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene IL17RB. In some embodiments, the sequences from gene IL17RB comprise or consist of:











(SEQ ID NO: 876)



TAGGTGCATTAGAGAATGGGTATTTGGGGCTTTACATTGAAAGCA







CAGTTGGACTTTTAGGAAGCCTAATATAGGCAATAGGTCATCGAA







GACCAGACACAGGGTGACCCATTGCTTTATTGGCTGATGCAGATG







AAGTCTCTGTCTGCTCGAAGCAGAACTCTGGGGCTCTGTGGGAAT







GAGGCCGTGTTTCAGACAGGCTAGCCTCTTGGCCAGTCAAGATGG







GACTTGCCATACAAGGTGGAAAGTGATGCCTGCCACTCTGCAGAG







GGAGATCAAGGCCGTGGAAGGAAGGTCACTTAGAGTTGGGACCGA







GAAGCAAATGTTTCATAGGAGAGCGGGTGTTTGAGATGGTGGCAT







TTGAAGAATGGGTAGGCTTCTGCCCAAAAAATGTGGGTAGGAAGA







GTGAGCTGGAGGAGAGATGGCACCAGCAAAGGTTAGGAGGTGGGA







AAGTGGGGGCTGTGTGGGCCAGCTGGCTGGAATCCAGGGCCAAAG







CCTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene DNASE1L3. In some embodiments, the sequences from gene DNASE1L3 comprise or consist of:









(SEQ ID NO: 877)


GTGAGGACTGCTGTGTCAGACGAATTCCCATCTAGCAGGGGCAGGCTCT





GGTCCCTGAGTGGGATGAGTACCTCTCCAGGGAGGGGGAGGAGGAAGGG





CATGTGCAGGCTACCACCACTTACAGCATTCTTTAAGGCCTTTCTTCAG





CCCTAAGTATCCCTTTTGCTAAATCTGCCTGGGTACCCTGCATGTGGGC





CTTGGGAATTGCCACACTTTCCCATTAATAACAGATGCCAACATTTACT





GAGTGAATAGCTAGTGCCAGGCACTGTGCTGAGACTTGACATGGACAAG





TTTATTTAATCCTCCCAATAATCCCTTGAGGTAGCTACAGTACCTTTCT





CATTCCCACTTTGCAATTGAGAAGTGTAAGGCATAGAGCAGTCAGGATT





GTTTCAGTTGCAAGGGACAGAATCTTAACATGGCTCATGAAAAAAAGGA





GCAATTGATTGGTTTGTGTAACTGAAATGTCCAGGAGTACTTCAGGCAT





AGCTGGATC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene DNASE1L3. In some embodiments, the sequences from gene DNASE1L3 comprise or consist of:









(SEQ ID NO: 878)


CTGGAATGTGCCTGCGAGCTGGAATGTCCAGCACAAATATCTTGGTTAA





GGTACAAGGACATAGAATGTACTATGTGCCTGTGAGCATGTCTAACAGC





TACATAGGATAGAGCTTAACAAAGAGTTATTAGCACAAAGCAAGGAGAC





TTGAAGGAAGTTAGTCTTTAAAGGAAACTATTATTTCTAACACTTATGA





TTTATTCTTTAATAAGAAGGGAAACTTTGAAGAGGAAACTTTTTTACTT





TCTACAACAGTCTACCAGCTTTCTCTTAGCCATGAAGGGCAACTGGGAG





AAATGAGTCTTTCGTGTACTAAAAATAGGCAGCCTAATGACCCCAAGAT





CATTAAAATCTCGGCCACTGGCTGGACCCTCTGGGAACTGGGGACCCAG





ATAATGCAGCCTGTGCCTTCTGGAGGGGACAGCTGGTTTCCCTCCAGCA





GTGTTTATGAGACCTTGACAGGGGAAGTGTGTGACCCCCGCCTCTTTCC





TGTTTCTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MYLK. In some embodiments, the sequences from gene MYLK comprise or consist of:









(SEQ ID NO: 879)


GTGAGTGCCCCCAGGAGCTGCCCCTCCAGGCCATGCCCCCATCCCCACC





TTCCTCCTGGCCCCGGCAGCTCCAGCCCAGCAGCCCTAGATTGTTTGTG





TCCTGCAGCTGAGGCTGCCAAAGCCAGAAGGGAGAGCCTGGAAGGGGAA





TGAGGGAGGGAGAGCAGGGGAAGGAGGGGGTGATTTTTTTTTTTTTTTT





TTTTGCACACACCCTTATAAGGCTACTGAAGTCATTACCGATGTAATAA





ACCAACTAAGCAAGCGAGCGCCTCTCACCAATGTTCCCATCTATAAACA





CAGCCTGGCCGGCCTCCCCCATCCCCTCCCGCTCCCCTCTACCCTCCCA





CCCTCAGGGATTTGCTCTCCCCTGAAGTTTCTTTCTTACCCAGCCCCTT





TTCCTCCTGCTACTTTCCTTTTTCCCTACACTGGTTGCAGGGGGAGGGA





GCCTGCCAGCTGCGAGGACCTCTGTGGTGGAGGAGTTGGTGCTGATGGT





GGTGGTAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MYLK. In some embodiments, the sequences from gene MYLK comprise or consist of:









(SEQ ID NO: 880)


TGAGTGGGGGTTAAGGGTGCTTCCATTCCCGAGCAAGAGAGGGAAAGGA





ACTACTTCTGTGTTAAGGGATCATGGAGTCAGAGTCAAGGTCACTGGGC





AGGCCCACCACCAGGGCCTATCTGTCTAGACCAACCTGCTACATCACAG





TTCCCAGCAGGGTTAGCCTTTCTCCTTCCCCTTTTATTGGTTGAGTGAG





TGAGTGATTGATTGAATGATTATATTATATACTTACAGAAAAGTGTTCC





TATTTCTGGATGCCTGCCTTAGCTGTTGTCCAGACATTCTTTTTTTCTT





CTTCTTTTTAAATAAGAGCTGAGAAAATAAAGGGTGACCACACAGTGAG





AGGGTCCTGCCCTAAATCTAAGGCTGGGGTGAATGTCTCAGTCAAGCCG





GCGACTTCCATCTTCCCCGCTGGCCAGCAGCCTGTGATCAAGTCCACAC





TGAACAGGCCCTTTGCCCAGAGGGCCTCTTGATCTTTATTGATCTTTGG





AATTCTTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CDV3. In some embodiments, the sequences from gene CDV3 comprise or consist of:









(SEQ ID NO: 881)


TCGCCGCGCCCGGCAGCGTGAGCGCAGAGCCGGCCTCGACCCCGAGCTC





GGAGCCCCGCGGGCCGCGCCCGCCGCCGGCCCCACCCATCCGGGTCGAG





GAGGCCGAGGCCATGGCTGAGACGGAGGAGCGGAGCCTGGACAACTTCT





TTGCCAAGAGGGACAAGAAGAAGAAGAAGGAGCGGAGCAACCGGGCGGC





GAGTGCCGCGGGCGCAGCGGGCAGCGCCGGCGGAAGCAGTGGAGCCGCG





GGTGCGGCGGGCGGCGGGGCGGGCGCGGGGACCCGGCCGGGTGACGGCG





GGACCGCCAGCGCGGGGGCTGCGGGCCCAGGGGCCGCCACCAAGGCTGT





GACGAAGGTGAGGGGCCGGGAGGCCGGCACTCGGGGCCCGGGCCGCGCG





CCGGCGCCCCATGTGCCCGCCCCCGCCTGCCGGGGAAGCGTCCGGGAGG





GGCCGCGGAGGCCGGGCGGACGGGCGCGGGCCGCCACGTGACGCAGGCT





CTCCACCTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CDV3. In some embodiments, the sequences from gene CDV3 comprise or consist of:









(SEQ ID NO: 882)


TCTGGGGCCGCAGTGCCCAGCACAGAGCAGCGTTTATCGGGTGACGTCT





AGGCTGAGTAATTCCGTGTGCTGACCTCAGCCCAGTAGTTTGAGCTGAA





TTTTCGCTTTCAGCAGCCGCATTTGTAGCAGCTCTTTTGTGATGACTTA





GTCTCTAAGCCCGTGAAAGAAAAAGAAAACTCTCGGTGGCAAGGTTGAA





GTAGAAATGTAGTTGTGAAATTCCTATTGACTTCTTCCTTCGGCTTTTC





TTAGTTGCTTAAAGTAACAGCTCCCATGCAGGAACCCAGCGGAAGTATA





GTGTTAGCCTACGAGCATGTTTGTCCTCCATTTAGCCTAGTTTGCCCCA





GAACGAAGCAAGGGCTTTGACCAGAAGACAAGTACATACTTAGTTTAGG





TTCCAGCCACTTGATCCACTTTTCGTAGTGTGTTATTTGCTTAGTTATA





TGTCTACAAATAGCTTTAATTGATCAAATGGCGTTTGTTTTACAGGACG





AAGATGAAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PLSCR4. In some embodiments, the sequences from gene PLSCR4 comprise or consist of:









(SEQ ID NO: 883)


GTATGTATAATTTTTGTAGTGCTCAGCAGTATTTTAATCAGATGGAAAT





TTGCATTATCCAATATCTAGTTCCTTCTTTATATTGATCATGTTTTAGT





GGCTCAAAGAAAAAAAAACTGCTCTATTACCCTATCTTCAAGAAAGTAA





TAAAGTGAATTATTTTGTATAATTTTATCCTTCTATACATCATATAATC





ATAAGCTCTTAGATTTGAAAAGGGCTTACATTTTATCTGGTCAATAGCC





CTACACTTTACCCATTGTTCCTACTCCACATTTACCTTTCAAAGATGAG





TAAAAAAAGGAGCCAAGAGACTAAATGAGTTAGCCAGGGACACCACAGT





CAGCTAATCACCTCCAGATCAGGAAATAGCATTTAAACCACAGTCAGCT





AATCAGTGGCAGAGCTGGAAATAGAATCCAAATCTGCCAAATCGTAGTG





CAGTGCCCTCGCTTTGGAAGTAAGAAGAAAAGATCTCAGTCAAAATGAT





GCCTGTTCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PLSCR4. In some embodiments, the sequences from gene PLSCR4 comprise or consist of:









(SEQ ID NO: 884)


TAAAATAATATAAAATGGGGACACTCCATGTGAGGTTCACCCTGGCTTG





TTTGTTCTCCTTAAGCCACTGAGAGACTTCCCTTTTCTATGGCTGGTCC





GTGTTAATCGTTCTGAACTTAACTAAACAACTACAGGCTATGGATGACA





TGTAGAGAGACCAGTGAGTAGGGACTTATCTAGGAACAATCTATAAGCA





CAGCTTCTTCTGATGTGATTACTACAGCAAGTAGATGTTTTGTTTTTCT





GATATAGCAGACAACTTTTTTAAAGTGGTACTGAAAGAAAGTGTTTTTC





TAATATGGACAACTCTGCTAATATCCATCCGATCAGATGCAGATAAGCT





AATTTCAGTTGGTAAAATTTTTTTGATTCTATTGACTTTCAAAAATATA





TTTTGGTATCAATTGATGCATTTTAATATCTATTTCATCAGTTTATTTT





ACAGTGGTATTTTAGTTCTGTTATTTCTGTCTTCTGATTGCTTTGTTTT





ATCCCAACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene BCHE. In some embodiments, the sequences from gene BCHE comprise or consist of:









(SEQ ID NO: 885)


GTGTGTCTGTTTTTAGTGTTTGGTTATTTAATAGATGATGGGTTATGTA





AAAAAGGGATGAAGTATACTCTCCAAGGCACGGTGATGGAGTTACTATA





TGTATCTCTGACTTTAAAATAACTTGGTTTAATGAACACATTATTTTTA





AGACTTTCTTCTATCAAGTTTAGATATGTTTTGAACTCTTACTATATGA





CCAGTACTTTAGTAGTTAAATAAAGAAAACTAAAATATAGATTTCACCC





TGTGGAAATATAAAATAGAAAAAGGAAACTAAAATAGAGCCCTACTTGT





TATTCAGATAATCTTTGTGGAATTAATTAATGGACAAAGAAATAAATAT





ATCAAATAATCATAATGTATGGAACTAAAAAATAAGATGCATAAATTGG





TAAAAACAGATTTATTGATAAATAATAGAACCTTGATTTAAATGGAGAA





AAAAACTCCGAAGTTTACATATTTAAAAACACAATAAAAAATTTCTAAA





TATTCTGAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene BCHE. In some embodiments, the sequences from gene BCHE comprise or consist of:









(SEQ ID NO: 886)


TTTCCTGGAGTAATGAGATTATTACATAATAAGATTTGTAATTAAACTT





GGTCTTAAACTCAAGCAGTATTAATAAGAGTATATAGAATGAAATAGAT





GCAAAAGATGAGTAAGTGATATGATTCCTTATTGTGAAATTTTTGGGCC





TAGGATAAGGGAAGATAATGAAACAAAAGTAACACCACCCTTTGAGCAC





GTTTAAGTGTGAATGAAGATATATTTTATTGAAATAAAAAATTTCTTCT





TCCAATATGTTAAGCAGAAATGCATATAAAAAATTCAACTTTAAATTTC





AATTCTTACAATTTTAAATAACATAGTGGTTCTCAGTGCTCAAACCACT





AAGCCCAGTTCACATACGTTTCATATCATCAATATATATCCACATTTTT





TCTGTCTTAAATTCTTTAAAGCTCTTGTGAACAGTGTTAGAAAACAATA





TTCTTTTTAATCTAACAATGATTTCAATTTTACTATAATGTCTCTATTT





TATTATTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MASP1. In some embodiments, the sequences from gene MASP1 comprise or consist of:









(SEQ ID NO: 887)


GTGAAGTTCACTTTATAACCTACAGACAGTGACACCTCAGCTCTGCACT





GGGCCTTCCTGCTCCGTATTGTTCCCTTAGGGATTTTGAGGCTTGGGTG





GGGGTTGGTGAGTGGGAAACTAGGAGCCCAGACCCACCTCCAAAGTTCA





ATGGGACTTTGAACCCCAGAAAGCTTGATTTGTTTGTTCTACATGTCAT





GGACTCTAGCCTCTAAACACGGTTAAGTTTTTCTGGTAGCTTATTAAAG





GCTTAGCTTATGAAAATTCTCCCAGAAAACAGCAAGTTTAGACACCCCC





ATGGTGACAATCCAGCTGTGCACCATGAGAACTGTACACCAAATTGGCC





AGAATGAGCCAGGCTCTTGAGAACCACAGTCCTTGCAGTAATGAACTTG





GCAGTTACACTTAGAAGTTCATATTCCTATCAGTTCAAGGAAAAAAAAA





AAAAACACTTAACTAAAAGTAAGGAGCCATAGTTTCAAGTCTTGGCTCC





CACTAACTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MASP1. In some embodiments, the sequences from gene MASP1 comprise or consist of:









(SEQ ID NO: 888)


AAGGGGTTTTAGAGAATACCAAGTCCAGCCTCCTCATTTTATGGATAGG





AAAACAGAGGCTTAGAAGGGGAGCAGAGAGGAGGGCTGGCTAAAGGCAC





CATAGCACATAGTGGACTAGGCTGGGCTTTTTATTTATTCGCTGTTCTT





GATTGAGCACCTACTATGTGACAGCATTGCTATAGGCACTGTGATATAG





CAGTGACCAAGAATGAGATTCTGCTCTCATGGACCCTAGGTTTCAGACT





AGTTGAGGGTGGGACAGAATAGAAAACTAAACAAGTAAGCCAGTAACAC





ATCATAGAAACCAGTGCCCCTAGCTCTGCTGCAGGTAATCTGTAGCCCA





GCTGCCTTTCACGTCTTCCTCCCCACCACACCACCTTTCTCTGAGAAGA





ACAATCCAGTCTTTCCTCCACAGAAGAGGATGATGGAGCTGCAAGCTGC





ACCCCTCCAAACACCCCACAGATAGCTTCTCTGTTCTCACAGTATCTTC





TCTCCTCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene IL1RAP. In some embodiments, the sequences from gene IL1RAP comprise or consist of:









(SEQ ID NO: 889)


AACCCAAAAGGACCTGGCCCCAGAAGCTTCTCTCTAAACTGATACAGCA





TCCTGGCTCAGAAGTTTAAGCAAAAGAGACATTTTGTTTTGAAGAGAGA





TCTATAAAAAGAAGCCAGTATTGGGATTTGGTCTAGACAATGGGATAGT





GTGTAGCGTAGATGGTATAGTATAGTGTTAGAATCTTACCATTAATACT





TTGTAATCACTGCATTATAGGTGGCTCAGCTTATCAACCTTCCTCGCCC





TCACCTGTAGTGGGGGTCTGTGGTTGCTTACTTGTCTGATTCCTACACA





GTCCATAGCTCTTGAGTCACTCTCAAATGCTTTGATTTTCTTCTCTCTA





TTTCTAGTATAAGTCATAGTAGAACAGAAGATGAAACAAGAACTCAGAT





TTTGAGCATCAAGAAAGTTACCTCTGAGGATCTCAAGCGCAGCTATGTC





TGTCATGCTAGAAGTGCCAAAGGCGAAGTTGCCAAAGCAGCCAAGGTGA





AGCAGAAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene IL1RAP. In some embodiments, the sequences from gene IL1RAP comprise or consist of:









(SEQ ID NO: 890)


TCAGACTGTGTCTAAAACTTTCTTTGTGTCTCTCACCTAACACAGAATC





CCAGAAAATCCCTTCAAGGATGTTTTCAATGGTCTTGAGAAAAGTAATG





ATGAGTTATCACACATGAAATCATGAAATCAGGTCATTCAGAATCCTCC





TGGCTCCTAGTCTATAGAGTGTGTAATATTTCTTCAAAAGTGAATAACA





TAGCACATAATTGTTACATTTTACAGAGACTTAATGCTTTTTTAAAGCT





GATGAAAAACAGGATTCACCTCAAACTTTCATTACAACTTTAAAACTAA





ATAGCAAAGGCCTGAAATGTATATGCTGCGTATGAAGTACTAGTGAATC





AGTTCAGGGTAATTCATTGCTTCTGCTCCTCAGAAGCCAGTGTGAAAAG





GAAAGACTGAAGTACATGATGTAAGGCAGTTTCCCAAATTGAGTTCTCG





GGAACACTACTAAGAATCAGTATTGTCCTGCAATGCATTATTGTACTCT





AGAATCAGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PI4K2B. In some embodiments, the sequences from gene PI4K2B comprise or consist of:









(SEQ ID NO: 891)


ATTTAGAATTCAAAGAAAGAGTCTTAACAACTAAAAAAAAAAAATGAAA





AAAAATTATTTATTGTATTTTATGTCATAAAAAGAATCTGGAAAAGTTC





AGATAAAATTGCACTGTTACTGAATAAGCAGGATAGGTTTAAAATTTGG





CCTCCATAATTAAATTCACCTTAATGAATATTTTTAGAAAAACGGCATT





CTTTTTCAGAGTGTCACCTTGAAGACATGTGTTTATTCTTTTTTTAAAT





TTTCCCAAAATAGCTGCTATGCTCCTTGAAAGTCTAGGGGTAAGCATTT





TTTATTTGCTATGTATTCTTTATGTGATTATTTAAAATTAGTGTATAAA





AATGGTTTTCTTGATAAATGTGGCTTATCTAAATTAGTGTTAAGTTTTC





TTATTGTGTTTACATGACATATTTTTTGACAGTACTGCTGAGAAATATA





AATATTAATCCCCTTGTTCTTGTACTTTCTTTTCTAACTATAGTTCTTA





AAATTATAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PI4K2B. In some embodiments, the sequences from gene PI4K2B comprise or consist of:











(SEQ ID NO: 892)



TATTGAAGTTCTCTCATTTGTTCACGTAAATCTTGTTCATTTTCA







AACTATATTCTTGTTATCCAAATCAAAGCGGTAAACTTTTGTTGC







AGAAGTCTTGAAGCCACTTTCTCTGTCTGTCTTGGCAAACAGATG







TAGTATTGACTCTCCTGGGCAGAATTCATCCTGTGTGGGATAGAT







TCTGACTCATAGCAGGCTCCTAGATCAGTTCCTGTCCCCTAGGGA







GGAATTTCCTAGGGGCAGGGCAAAGTCAGATATTGGAAATGTTAT







CTGCATAATGTGAGTAATTAAGGCTTCATTGTATAAATTATGTTA







GGTGCTTATTTTGACATTATGTAACTCTTAGTGTAGTCTATAAAT







ACACTAAACTCTTAATTTTTCCTGTGCTATAAAGGAGCAATGACG







TAGACCAGGGATGTCCAGTCTTTTGGCTTCCCTGGGCCACACTGG







AAGAAGAATTGTCTTGGGCCACACATAAAAACAGTAACACTAATG







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SULT1B1. In some embodiments, the sequences from gene SULT1B1 comprise or consist of:











(SEQ ID NO: 893)



GTATACTCCAGACTCCATGTCAGTTTTTTAGTATGGCCCTATCCC







TTTACCCACTCACATAGTTTATTTTTTAAAATATTTTTTGTTTCT







TTAACCATATACTTGCAACAATCTATACTATACCCACTCACGTTG







TACGTAAATCTTCAAAAGCCATTAAGTTTTAGTCTGAGTTTATAT







ACTCAAGTTTTCCAAACTTGAGCAGCAACAGAATCCAAACCTCAG







CACAATATTACTACTTTTTTTTCCAACTCTCCCTAGATCTTCAGG







CCTTCTGTAAAAACTGAACTTAAGGACGTTAGTCCTTATTTGAAG







ATTTGGTATAATTCTTTGTTGTAACATTTGTTCCTGCTTATCTGC







TTTTGGGGAAATTTTATACTTCAAATTCTATTTATTTAATAGCTA







CAGTGCTATGTAGATTATGTGTTTTTTTCTTATCTGACTTTTGAC







AATTTGTGATGTGTGAGAAACTTTTCTAAGTTGTTGACTTTATGA







ATGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SULT1B1. In some embodiments, the sequences from gene SULT1B1 comprise or consist of:











(SEQ ID NO: 894)



TAATGAAAATATAGATAAAACAGCTGATATTATATACAAAGTTAA







CATCATCTATGCTACAGTACCTAATACAACACGATAGCCAGTTTT







AGACTTACTAGTTATTGATACCTCTTTGGCATGAAGAAAACATTT







AAATAGTCATTTATAGCAATGCACAGGTATAATAAAACTCCAAAT







TATTAAAGAGGGTATTATTATCTCTCCTTTTGGAGATGGAGAAAG







TGAAGTACAGAGATTCTGAGATACTTTCAAAGTGTCTCACAATGC







TTAAGTGATAGAGTTAGCATTTGAACTCAGGGTGGCTAACCTCAG







AATGTGTATCCTTGAATGTTACACAATACTGACTCTAGTAATGTG







GTGAAATGCCTGTGATTGTTTCAGAATTTATCTGCATTACATAAT







GCTCAAAAATGTCTTGAAAAACTGGTTGGCAAAATAAGGAAACGT







AGCCTTTCTGGAGACTCTAATATTTCCCTATACTTAACTAAATTT







CTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CXCL2. In some embodiments, the sequences from gene CXCL2 comprise or consist of:











(SEQ ID NO: 895)



GTAAGTCTCGCCCATCGCTGCCGCTGCCACTGCTGGGGTCCCCGA







CTCTCCCGCTGCCCCAAACCCTGTCCTCAGCCCGACCTCCTGTCT







CACGAGATTCCCTTCTCTCTGCAGAGCCACACTCAAGAATGGGCA







GAAAGCTTGTCTCAACCCCGCATCGCCCATGGTTAAGAAAATCAT







CGAAAAGATGCTGAAAAAGTAAGTTATAATTTCCATGTACACAGG







CGACTGGAGCTGTTGGTCAGAAATACTGGCGTCTGCCCCCTAAAA







AGTAAATCAGGAAAACCCAGGGTTAGCTGCAGGACTGAAAAAATT







ATTATTTTCACAAAGTTGCCATTAAGGTTATTAATCTGTTCTGGT







GCCAGAGGATATTCCCAGTGCCCAGGGTCACCACCCAGGTTCTTC







CCTCCTGCCAGTGAATGTAGGTAAAACTGCTTTCATGTAAGGTCT







CATTGGAGTTTGCCAGTTGTGCTGAAGTGTTTTCCACCTGTTAAA







AAAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CXCL2. In some embodiments, the sequences from gene CXCL2 comprise or consist of:











(SEQ ID NO: 896)



ATTCGGGGCAGAAAGAGAACATCCCACAGTTGGCGGGAGTTACGC







AAGACAGTCAGACCCGGACGTCACTCGTGAGTGCCCCGACCCCCC







TCCACCCCAGAGGCGGGGCCATCGCCTTCCTTCCGAACTCGGGAT







CGATCTGGAGCTCCGGGAATTTCCCTGGCCCGGGACTCCGGGCTT







TCCAGCCCCAACCATGCATAAAAGGGGTTCGCCGTTCTCGGAGAG







CCACAGAGCCCGGGCCACAGGCAGCTCCTTGCCAGCTCTCCTCCT







CGCACAGCCGCTCGAACCGCCTGCTGAGCCCCATGGCCCGCGCCA







CGCTCTCCGCCGCCCCCAGCAATCCCCGGCTCCTGCGGGTGGCGC







TGCTGCTCCTGCTCCTGGTGGCCGCCAGCCGGCGCGCAGCAGGTG







GGTCCCGGCGCCCTGGGGTCCCCGGGCCGGACGCGGCTGGGGTGG







GCGCCCCGCGCCGACAGCCCCGCTCAATCAGCGAGTCTCTTCTTC







CCTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene HSD17B13. In some embodiments, the sequences from gene HSD17B13 comprise or consist of:











(SEQ ID NO: 897)



GTAAGTACAGCACAGAACACCCAAATACTAAAACACCAATAGAGC







TTTTTTTTTTGCTTTTTTTTTTTTTAGACAGAGTCTCACTCTGTC







ACCCTGGCTGGATTGCGGTGGTTGCAGTGGCATGATCTTGGCTCA







CTGCAACCTCCGCCTCCTGGGTTCAAGCAATTCTCATGCCTCAGA







CCCCCAAGTAACTGGGATTATAGGTGTGTGCTGCCACACTACACC







CAGCTAATTTTTGTATTTTTTGATAGAGACAGGTTTCCCCATGTT







GGCCAGGCTGGACTCGAACTCCTGACCTCAAGTTATCCTCCTGTC







TCGGCCTCCCAAAGTGCTGGGATTACAGTCATGAGCCACCATGCC







TGGCCCAATAGAGCTATTATTATGGAGCATCTTTCAGTTGTGAAA







ATTGGCATGGAAACTCTCCATCCCTGGGGAGAACAGTTATTTCCT







CTGTTATTTTCCTACCCAGTCTATAAAAAGAGAGTGATTCATTTT







CTCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene HSD17B13. In some embodiments, the sequences from gene HSD17B13 comprise or consist of:











(SEQ ID NO: 898)



TTTACCCAGACCCTATTCAAGATGGAGTTGCTCTGATTTAAACGC







CTCTGACAAAATGACGACCTCAAAACAATCCAGCTTTATGGAATA







CCTCCACAAGAAAGAAAGTATACTTAGCTATAGAATTTTCTCCTT







GCATCCAACAGGCTTTGAGATGTCAGATGTTTCCTTCCTGTCCCA







TGATTAATCCTAGCCATTCCTCTTTCTTGTCTGGCTCCACTACTC







CTTACCATCTAATGCCTCGCCACCATTTTGATATTTTGACTAAGT







GAGCTATGAAACACACCTACTGGATATGAAAGTATAAGTTTCTGA







TAACAAAACATCAACATGGGATGTGGAGGAAGTGGGTAGGGTGGC







ATTAATGCAGCAAATCCTGGAATATTTTAAATCTTCATTCTAAAT







TTAGTAAAAATATAGGATAATTTTCCTGCCATCATTTACTTATAA







AATTAAAATTTTAGAAAATAAAAATAATATTTTCCTCTTTTTAAT







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ADH4. In some embodiments, the sequences from gene ADH4 comprise or consist of:











(SEQ ID NO: 899)



GTCAGTTTTTTTTTTCTTCATAGCTTTAAATTCTTTTCCTAGTAG







TTCTGAAATTCTTAGAGTGAAGGTTATTGAAAAGAAATAAAAATA







ATTTATTCAACCATTGAATCCCTAGAGCATATCCAAATACTACAT







AAAAACTATTAATGACTTATAAATAATTTATATAAAAAGGTAATT







ATAATTTGCAAATTGAAATTTAATTTGCATTTGAATGAACCAACA







TGGAGGAACAAAAAATAAACATTGACCTTATTGAGATAATTTCTT







ATATAGAGTGGAAAATAATCTTAAATTTTTCTTATAAAGAAAAAT







TTTAAAATAGATATAAGTACAGAAAATTAAAGATGGAAGGAGTTT







AATCTATTTGAAGCAAACCAAATCTGACCTAATAATTTCATTCAT







TCAGCAAATGCTGATTAAACTACTTCGTGTCAAGTGCCATGTCTT







TATTTTGCATTTTGTCCACATTACAGGTTGGAAAAGTGTAGATTC







TATC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ADH4. In some embodiments, the sequences from gene ADH4 comprise or consist of:











(SEQ ID NO: 900)



TTTTTTTTTTTTTGAGACAGAGTCTCACTCTGTGACCCAGGCTGC







AGTGCAGTGGTGTGGTCTCGGCTCACTGCAACCTCCTCCTCCTCC







CGGGTTCAGGCAGTTCTCCAGTCTCAGCTTCCCGAGTAGCTGGGA







CTACAGGCAGGCACAACCACACCCAGCTAATTTTTGTATTTTTAG







TAGAGACGGGGTTTTGCCATGTTGGCCAGGCTGGTCTCGAACTCC







TGGCCTCAAGTGATCCACCCACCTTGGCCACCCAAAGTTCTAGGA







TTACAGGAATGAGTCACCTGCACCCGGACTGTGCTGAACATTCTA







TTTGTAATGGAACACTGGGCCCTCAAGGCTCAATCTACAATGATA







CTATGATGTCATTAGCTTGAATCCCATTATCTTACATGCAGAGTT







GGATTAATTTTTTTCTAAAGCATTGTACATTGCCACACTGGAGTT







CATATTCTACTTTTCCACTGACTCAAGTATTCTTTTATGCTCCAT







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ADH6. In some embodiments, the sequences from gene ADH6 comprise or consist of:











(SEQ ID NO: 901)



TTTGAATGTCTTGTTATGGTGTAGCTCGTTGCAAAATTCACCTAA







TTTTATCACAAATGCAGGAACTTTTTTTCCTTCCTGTTAATATAC







AGTCAAACATATTACCAAGTACTGGAGACTATCTATATCACTGTA







TAACCATTCACTGTTGTTGCCAGCTTCAAGACCACATTTGAATCC







TATCAGGCCCAGTAAACTCTGTGAATAGACTCTGTGAACTGTCTC







TAAGTCTCACTGTTTTGTCCACATTAAGTGTCTTGTTTGAAGCAC







AAAGTAAAATCTTTCTTTCCAATCCCTGTCACTGCACCTCCCTAG







ACAGTCACTACATGGGGCAGTTTATGGACTTCAGCAATGTTTACC







TAACTCCAGATCCACACTATAGGAGTCCAGCCTTCAGGGAGAACT







AGTTTTTTACATTTTTTGCCTGCATAATGAGAAGCTTTAAGTTTT







TCATATTTTTACCTGAGAACAAGAATAATTACATTCTAGGCTATC







ACCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ADH6. In some embodiments, the sequences from gene ADH6 comprise or consist of:











(SEQ ID NO: 902)



AAAATACATAATCCTGAAAGTATTTAAGTGTTCACCTACCGTTAC







TTTTGCCATTAGCATTGTATTTCCAATATGGATTTTTTTTTTACA







TGACATATTAATGGAAGTCAGCTTATAAAATTGTAGCTCAAGTTT







AAAAACATAAGAAGGTAGTTCACAAATAGGCCATTGTTGTATTTG







TTGTTCTACTTTGGGGGAGAAGAGGAATTATTCATAGGATTCCTA







ATTCTGAATCCTATCTTTGACCCAAAAAACAGAAACCAGCCATAA







CCCAGAAACTGGCATTCCCCACACGAGTATAAGCTTGCTTACCTA







CTTTTTACCCTTGGAGTTTCATGTCATTTTAAAAAATCTTCCCAT







GGCTTATTTAATCTTCTCATTTTTCACCTAAAGAGAAAAAAAATC







TAGTTTACTGCTCTACAAAGTGAGAATTTGAAATCCAAAACACAA







ATGTTGCTCTAAAAATGTTCCAACCTTTTTTCTCTTCTTCTTTTT







TTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ADH1A. In some embodiments, the sequences from gene ADH1A comprise or consist of:











(SEQ ID NO: 903)



TACTTGTGTCATCTTTGCCATTGAGAAAAGCTATTTCCTATGACT







TCCTGCATTTTTGGTATCTTCATAATCTTTAGTCATGGAATCCCA







GTGGAGGGGACCCTTCACTTGCCCTGAATATACACATGCTGGGCT







ATTGTGCTTGTAGTCTCCTGCATCTATCTCAATTTTCACTGTCGG







CATTTTCGCCTTTTTTTTTTTTTAATAAAATATACCGAATTCCTA







GGGTAAAAGCTGTAGTAGGGTAAAGGATATACTCATATTTACAAG







TGGTGAAAGTCCAAGAGTTGTAAATCCAGGAAATTTCTTAGGAAC







TCAAATAAAATGTCACATGTTTTCTTACAGTGAAGGAGAATGTTT







TTCTGACATTTATACTATGTCTTTATGGTCAATATACAATTGATT







TTTAAAAATAATAGCTGATTTCATGCTTCATATGACTAAGGTACA







ATTACTAATTGCAAAAACTGAACTATTCCTGGAATTATGTTCAAA







AAAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ADH1A. In some embodiments, the sequences from gene ADH1A comprise or consist of:











(SEQ ID NO: 904)



ACTCTATACTAGATATGCGACCAGACCAAGTTACAAATCTTTCTA







AGTCTCAGTGTTTATAATCTCTAAAATCAAAATAATATGAAAATC







TACCTCCTAGTGTTGATGTTTAGGTTGAAGTTTAAGACAGAAAAT







GCATGAAGAATGTGACAAATAGCAAAAACTTATCACAAGTTATTA







TCCATTGTTGTTTTATATTCCTTATTATAACAGGAACGTGGGCAC







ACAACAATGATTTTTTGAAGATGCTAACGTAGTATTGAAGTCTAA







ATGACAGGCATACTCAGAATGTTGTGAGTGAGCTTAAAGTTGAAG







AAAACACAAGAGAAAACCAAAAAAGATGAGCTTTCCTTTGACTTC







ACTTGACCCGAATGCATGGGGGTGGGATTAGAGTGGCTGAGAACA







GACAGATTTTCACTAAGTCATTTCAAATGCTCCTTGGACTCTCAC







ATGGAAGCATGTCTAAATGCTAATACAATGTCTCTTCTTTCCTAT







TGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene NDUFC1. In some embodiments, the sequences from gene NDUFC1 comprise or consist of:











(SEQ ID NO: 905)



GTAAGTGTTAAGAGATCTTTTCAACTGTAAAATATAATATGTCAC







CTTGGATGAGTAGCTGTCTTCTTTAAGCTTCATCTGTAAATTGAG







GCTTATAACCACGGCATGATATGTGCTCGTTTTGAAGATGAAATA







AGACAATGAATGTAAACCATATGATACATTAATGCACCGTGCCTG







GACCACAGAATTCAATCCAAATGCTAGTTATTATTCAGCACGACA







CTTTTCTTTCTTCTGGCTCATGTAGGAAAATTTTAATAAACCTCT







TGATGAATAGCCGTAGAGATGGGGTGAAAAACCCCCAGAGGACAG







AGAATAGGTAAACTCTTTTTTCTTTTAATTATTTTCTTCCTAATT







CCACTGTATAGACAGAAGGCTGAGGCCGGGGTGCTAGAGAACCTT







GCCGTGCTGGAATTCACGTTGACGCCCCCACGGAGCTCTGCTGCA







GAGCCCTCGAGTCCCGCACTTCCGGCCGCCAGGTGGCGCTGGTTC







TGTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene NDUFC1. In some embodiments, the sequences from gene NDUFC1 comprise or consist of:











(SEQ ID NO: 906)



TTTTGGTTTTAAAATTAATGACATAAAGTTTAAAGGATTAGTTTT







AAAGGGTCAAAATGTATTGTCTGTTACCTATAAATATTTTTACTG







GTCTTTAGACATCAGATTGAACTGAATTGAATGTACAAAGATCTG







GCTCAACTGTGCAAGATAAAACCTATTCCTCAGAACGATGTCTTT







AATAGGCAAAACCAAATAAATTGTTTACTACTACCAGACACTTGT







TTAAGCTTCTCAGCAACCCTATAGGTTTTATGTTACAGATGTAAA







ACTTAGGTATAGAGAGGTTAAGTAATTTGCCTAGAAATCATAGAG







CTAAAAAGTGGTGAGCAGAATGGCTTTGAACGCTGATTCTACAGC







TTATGGTCTAGGGTGGTCCAAGTCTGTTAGGTGGGCTAATGAGGA







TCACTAGATCTGGAGTCAGAAGACCTCAAACTGTATCCCACCTCT







GCCATCTGTGTGATCTTAAGCCTACCTAAGGCACTAATTCTACTT







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SORBS2. In some embodiments, the sequences from gene SORBS2 comprise or consist of:











(SEQ ID NO: 907)



GTATAATATCACTAACTCACACAATGTCGCACTTGAATGGCTTCT







AGCTCTTCTGACTGTTTTTGTTCTAAACGTAAAAAGCAAAGGAAA







ATGAGATCTTGTAGGAAACATTTTAAATGTGTAATTAAAAACAGC







AGTCACTTGCCAAAAATAGGTTGAGTAACTGCGAGTGTTAATAAC







TATTCACGTTTTTGTTATCTCATGAACTTAAGTAGCATAATATTT







CATTAAGATAACTCAGAGGTTTTGGGGTTTTTTTTTTTAACCTAA







AAACTTTTGGGGTAGGGGTACAGGGGTACAGGATGAAAAAAGGGA







TTTGTAGGAAACTGAATCTTCATTAACTGGATTCTTTGTCAAAGG







TTGTGAACAGAATGATAGAACATTAGTTTTGAAAGAGACCTTTGC







TTTCCTTTAATCCAATCAGCAAATACCCTTCTACTATATATATTT







AAACATACCAAAATCTTGACCAATTAAGATTCACATGTACCCACA







GATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SORBS2. In some embodiments, the sequences from gene SORBS2 comprise or consist of:











(SEQ ID NO: 908)



TTTACTGCAAGAATTATTCAAGCTCTCTTTGGGAATTATAACAGC







TAGAAGATGGTAAAACTTAAATGATATTAACTTAAAAGTATGTTG







TACCATAGCCTTTAATCCTGACATGGAGCTAATCCTTGACTCTAA







AAGCCAGATCCAGTGCTTATAGATTTAGTATAAATTGAATTTAAA







TGGTATAAACAAAGTTGAGTTCTGTGCCTGAGTTTTCACAAAGAA







AACCATAAAATGTTAAGTATTAAGTCTTCCATGCTTTCACTAAAA







CAGTGATACCTATTTCTAAGACAGATCCCTGGTCTTCTTTTGTTT







TGTTTCGTTTTGTTTGTTTTAGTTTGAGGGTGGTGTGAAATTGGC







AGGTGCATTCCATGATTACTAATTCTAACTGTTCATCTTTCTCAT







GGATGTCTGGCACATTTCTCTTAAAACGCATGATATTGCACGTGC







TTTTCCCGTTGCCTTGGCCTCTGCTATATCATTTTTTTGCTTCCA







GATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene KLKB1. In some embodiments, the sequences from gene KLKB1 comprise or consist of:











(SEQ ID NO: 909)



GATATGTACACAACTAAAACAAAACTAAGATATAAGATCATATTA







ATGTAATGATAATAAAAGTGGATTGTCTCCTGTTCTAATTTTAAT







AGGCACAAGGCATTTTTGTTAATCACTTCTTATTAAAGAATTTTT







TATAATATTCAGGAAAATACAACAAAAAAACCCTTACATTCCTAA







GGCCTCAGAGTCAGAGTAATATAAAGGAAATGTAAACACATGCTG







AGTAATGCAAGCATTTGGCAATGGTGGTGACTGGAGCTGGGAGCA







CAGCTTTTATTTCTCTGAAATAGGAATTTGCCCCTTAGAGTTAGA







CCAATTTTGCCTTCCTCTAAATGGCAAACAGTTTGGAGATACTTT







AAAGGACATTTTTTCACTTAGGATGTTTAGTACTATGAATAATAA







ATAGTCACAATTTCCTTAACTATGGTGACAAAATACAAGCAAATT







TAGCCTCATGTCATTTCCTAAGGAACATCTTCTCTCTGTGAGTTC







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene KLKB1. In some embodiments, the sequences from gene KLKB1 comprise or consist of:











(SEQ ID NO: 910)



CGAGTATGCATGGAAAATCGCATCACAAAGGCGAGTATGCATGGG







GAGCACTTGCTGCTGTACTTTCATCACTTTTATAGTCTGAGTTCT







TAAAAGTTTCGTTCATTTCCCTCAAAACACTTGAACCTGCAGTTT







CAGTAGGTACTGTTCTGCCAGGTGCAGATTAGTTAAGAGATTAGC







AGACTTCTCTGCCTATCTTCTCTTACTTTAAAACAAATGTTACCA







TTGAATCAAGGAAGCAATAGCCATGAGAAAAAAGAAGGATCTGAC







GCCTTTGAATGAAGATTCAAAACATGATCTTCATGTTTTGTATTA







GCTTGGAGTAAAATCCACTTGCTGGCAATATAGCCCTTAAGCTTG







TTGCCTCTTCTCTTTGTTTCAGAAACTAGAGCCCTGTTTATTCTG







ATCAAGGCTCTGGCCCACTGTCTTTATCTCAGATAACCCACCCTC







TTCTGCACACAGCATGGAGCTAAGAGAAGGGTGTCTAGTTATGTA







ATAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene C6. In some embodiments, the sequences from gene C6 comprise or consist of:











(SEQ ID NO: 911)



GTAAGAGATACCCTACAGACTGTGTCTGGAAATTGGGAAAACCAG







TCTAGTGTAACCAATTTGATGGAAGAGCTGAGCTTTTTGAAGCAC







AGACATGAAATAAATGAATTTTCCACATAACTGATATTCCTGTGC







TCTGCTGAATTCCTCCTTCCCCATTGGGTAGCAAGTTTCTTAATT







ATCTCTCAGCCCAGTTCCCTGTTTAAAATGAGATTGTTCATTATA







ACAGTCTCATTTTGGTTTTAAAGACTTAACTTCTGCACATGTAAA







AACTTTAAACTCAGTTATATCAACACTTTCCTGTTCCTCTCACTC







AAGCCCAGGGCTTGGAGTGGGGTGGGGAAAACAGGCAAAAGAAAA







CTGGTTGGTGAGTGAACACACTACATTGGCTACGTGCCCCCTGTT







TTTTTCTGATCTTCATATAGAAATGCTTGCTTTCCTCTCTTGGGC







AGCCATCATTCAGAAGATCTCTGTGTGTTTGACACAGACTCCAAC







GATT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene C6. In some embodiments, the sequences from gene C6 comprise or consist of:











(SEQ ID NO: 912)



AATCTTTCTAGCTACATCAATTTTTTATTATATGCTTCTCTTTGA







TAGCCCTAAGAAAGGCAACAATTCTATATTTATATTAGTGTGCTC







ATTTGATTAATGTCTGTCTGCACCCACAAGACTATAAGCTTGGTG







AGCACAGGATCTGGGTCAGTTTATGCTCAATCTACTCTCTCCAGA







ACCTTACAGAGTGATGAGAATATCATGTATACTCAATAAATATTT







GAAAAACAAATGAACAAAAATTTCAACAGTTCCATTTTCTTTAGT







TATCTGAACATAATCGCAATTTTAGGTTGGAAAATGACACCTCAT







GTGCACTGGAAAGGACCTTTCAGGTGGAATATTTAATGACAATGT







ATTTAGAGAAGCCAAAATGAATGATCTAACTCCAAGAGATAAATA







TGAATCCTTTACCACTGCCTCTTCTCTGAATTCAAGCTTATCACT







TAGAATCCATGCTGTGCACTGTTCTTTTCTCCTTTTTTTTTTGTT







TCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MRPS27. In some embodiments, the sequences from gene MRPS27 comprise or consist of:











(SEQ ID NO: 913)



GCAAGTGCCCATTCCAGAGACTCACTCACCTGACATATTACTACT







GGAAGTTGAAAAGTCCCCACATTTATTTGTCTGAACTGCTTAAAA







AGTCACCAACAGGAATTCTTCTCAAACTTGCCATCCAAAATTGAA







CCCCGCTGGCCCAGTGTTTTGACTTGTTTATATTCCAGATAGCCA







CTCACGTAAGTTTATCTTTTTAATGCTGATGGGGGCCAAGAAAGG







AGTTCATTTGCTTCCTGGCTGGGAAATTCAGGAATTGCATTTTGG







GCAGCATATTTTATAGTATGTGTGTAAGTATTTCACTGCTTTTCC







TCAGTTTATTTTTTGTGAGAGTTCATTGAAAATGTCCAAGGCTGG







GCACAGTGGCTCATGCCTATAATCCCAGCTCTTTAGGAAGCCGAG







GTGGGAGGATCACTTGAGGCCAGGAGTTTGAGACCAGCCTGAACA







ACATAGCAAGACCCTGTCTCTACAGAAATAAAGAAGGAGGCAAAG







GCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MRPS27. In some embodiments, the sequences from gene MRPS27 comprise or consist of:











(SEQ ID NO: 914)



CTAGGCAAGCTCTGTCTTCACCTGAAAATCCTACTCAACACTGGG







GACAGAGATCTCTTTAGCCATGGGATACCAACTTTTAGAACTAGA







GCTAGAACTTGAGAGTACACTTTGTCACTCTTTCTTATGCTCCTC







CAAGAAATACACATTTCCTCAGAATTGTAAGTGTAATCACATTTT







ATAAGCTGAAATCTCAGAGTACCTTTAGCCTTCATGAGCACAACC







TTGTTGGGGATATTTGGGTTGTATTTGTTTCTCTTTTCTACACTC







ACATGTTTGCCTATCTTGACAGCCTGTTGGCCTAGGCAATAATAA







AGCTTCTGTCGAGGTGAGCAAACAGCTGATGGCATAGGCGCACAG







AGGGGACTCCTACAGTGGCTGGCTGTAATCTGCCAGGCTGGTGGT







CAGAGCAGGCGAGAACAAAAGCCAGTCTGTTTGCAGGCAAATTGG







AAAATCAATGTGTTAGTTGTATTCTTTTCTCTTTGCCTTTCTTCT







GACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene DMGDH. In some embodiments, the sequences from gene DMGDH comprise or consist of:











(SEQ ID NO: 915)



GTAAGCATATCATTTTTTTGAAATTAGTGTTTTTTAAGTGCCAAA







GAAAGAAGATATTTAGCCGTAATGTTTCGTACGATGGACATTGTC







ATGAAGCACTGTGATGCTCCCAAGTTAAACATTTTGTCTGTTTCT







AAGTGACAAAGCCCTCCCTCTATCACTTTGCTCTTGGTGGATGCG







CTATCACTCTGTCCTTGGTTATCCAAAAAAAGTTGCTTATTTTCT







CCATTCTGCATTTTATTTTGCTCCAGATTGTGTTTTTCCAAATTA







TGTTTATTTTTCCTTCCTGTATTCACTGTTTCTCTATATGAATAA







CCAGATTCTAGTGTTGAAGTTCATAAGCATCCTCTTTGTGTCCAA







TTTGCTTTTTAGGGCAGATTTGATCATTTGGGTTCTCTCTCATCC







ATTTCTGATGACTTTTGACTCTAGTTGAAAAAGTTTTTAAATGCT







TTTCCTTCTAGAACATTATTAAACATATTTCATAATCTTCTCTTT







TTGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene DMGDH. In some embodiments, the sequences from gene DMGDH comprise or consist of:











(SEQ ID NO: 916)



AATAGATGAGACCCATATATAATATTTCACTGATCATTCTATCAA







CGTATCCCATCTGAGTTTCATTCTTCTTTGTTTTCCCCACAGTAC







CTTGCCTGAAGGCCTAAAACAAAGTAGATATGCAAGAATTAATTG







TTGAATGATTGAATCAAACATAAATTTCCTGACACCAGATATATT







ATTTAATGTGAATTTATTAACAGTATTAAAAATTAAATATAAATT







ATAATTGATATATCAGTTGGAATTAGATTTGTCTTCAAATGTCAG







AAACCAAAATAAGAAGGTGTAAACAAGATAGAGGTTTATTTCTCT







TTCTTTTCAGCAGTCCAGGACTTGAATGGTGGCTTTTTCATGAAG







TCTTCAGGGACCCAGTCACTTTCCAGATCAAGAAACACTTTTGAA







GTCCAGTTTTACTAAACGTTTACTAGCAGGATATATTTTTACTGA







GTAATAAGTATATATATATTTGAGTATTGACTCTGGTATGATTGA







TTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ZCCHC9. In some embodiments, the sequences from gene ZCCHC9 comprise or consist of:











(SEQ ID NO: 917)



GAATGCTCAACGAGCACTGAAGGGGGGACATGAGGCACTGCCTCT







CACCCAAGAGAGCCCCCAAATGTCCTTTTCAGACTTTTATCACAG







TGACCCACTCCCACCGCGCCTTTACCTTGCCTCTGCCTGGAATGT







CCTTAACTCCCTCTTGCCGGCAATTTCACCAGATTTTTGATTTCT







TATCCAGTCTCACGGGAAGCCTTTCCGGCATCTCCCACCCTACCG







ATCTTCCCGTCTATCTCGCCTGCTACACTGTGAGTTCAGACAGCA







CGGATTGAACTCTTCATCTTTTCGTGTCGGCGTCCTGCACAGGGC







CATCCCATCACTAAGCGCGCCACAAACTCAGCCCAGTATTGACGC







CCACGAGTCGCTTGTAAAGACCGTCTGTGACGCCGGAAGTGACGC







GCTCTCGGGCACTATGTGCTTCCGGTCTCCAATACCGCAGGAGGG







CGGTCTTCCCCGGCTCGCCAACTCGGCTGCTCTGGGGGATTCGTG







CGCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ZCCHC9. In some embodiments, the sequences from gene ZCCHC9 comprise or consist of:











(SEQ ID NO: 918)



TGAGGTTCTCAGCCACCAAAGCTGCAGGGTCTCGTCGTTCGTTCC







CTCTGGCCTGGCGGAACTTGCGTGGGAAGCTGGGAGGACAGAAGG







CTTAGCCGCTGGGTCCCTGAGCTGGCTGGAGCGCGGGCTGTCTAG







GGGCGGCGACCTCAGCTGTTGGGCTCCGGCTGCTGGGGGCTGTGC







GTCCAGCCGCCCTGGAATCCGCCTGTGGGTGGGCCGCTTGGCTCT







CCCACTTCACCCTCTTGATAACCCAGAGCCTCCCCCTTCCCACGT







ACGTAGGGGAAGGATAATCTACTTCCTTTTGAACAGCTTCGGTCC







CCACTATGACAGTCGCATAAACTTCAGCGAAGAGTGCGCTGGTAA







AGTGGCATGGAGTTTCTCACACCAATTCATTGGGTCCACCCTTCA







GCTTCTGTGGAAGGGGTCTTTCTGTTACTAGGAGCTGAGACTTGG







GGGGTTGCCAGGGATGCTGGTCTTAAAAGGTGATAATTTAGGCCG







GGCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene LEAP2. In some embodiments, the sequences from gene LEAP2 comprise or consist of:











(SEQ ID NO: 919)



GACGATCTGGGGAACTGTAGGCCTTGCTGAAGCATCCTCCCACAG







AAAGGAGATAAGGGCCTTATCCAATTGGCTGTACACAATAGATCA







GTGCTTCTTTCCTAGACAAGGCTGAAAGGGGCCAACATTATTTCT







GAAGACTTCATTATTGGAATTCTATGGGAGTGATCTCACTGAGCT







ATTTTGGAATAGAAATGTGGCTAGTTGCCTGACCTCCCTCAATGG







TTTCACGTGGCTTTCAAAGGGAAGGAAGGGCAGTGCTGACTTTTG







GTAAAATGGGCGAAAGGGTCCATGCCAGCAACACAATCACTCAAA







GTCCAGATGAGGGATCAGTAAATACAACGTGCCTGAAAGGTGGCC







CTTGAGCACATTCCTCCGGTAGACATTAACTTATTAAATTGATTC







TGATTACAAATATAAACTTTGCCCCCATCTCACCCAGTAACAATG







CAAGAGTTGATGTCAGTCTATAAAAGGAAGTAGGAACTGTCCCTG







GCTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene LEAP2. In some embodiments, the sequences from gene LEAP2 comprise or consist of:











(SEQ ID NO: 920)



TAAGGAGGGAAGGATACTTATGTGTGTGTGTGGAGTGTGGAGATG







ATAGTGGTGGTGGAACTTGAAAGCTAGATTCAGTCCTGAGGAATG







GTTCCTCTGTTCTGAGTCTACAGCATCTGCGGAATGGAATGATCA







CTCTTCCAAGGTGTGCAGCAGGGTGTCAACACTTTCATATCTGAA







TGTCTTTGCCCTTACAGATAGATGGCTCCCCAATACCAGAAGTGA







GTTCGGCAAAGAGAAGGCCACGGAGAATGACCCCATTTTGGAGAG







GGGTTTCCCTCAGGCCTATTGGAGCCTCCTGCCGGGATGATTCTG







AGTGTATCACAAGGCTATGCAGGTACTCCCTGAACCTGGGAGCAG







GGTTGGGCCAGAGAGCCCTGGGAAGCTGGGCAGAGGAGTGACAAG







GGGACACATGAACCTAAGAATAAAGCTGGGATGGAGGAGTTCTAG







ACTGAGACTGGGAGCTCCATGGAAAATCCCTTAGGAGTTAGGAGC







CAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ETF1. In some embodiments, the sequences from gene ETF1 comprise or consist of:











(SEQ ID NO: 921)



GTAAGGGGCCCGCGCTCCCCGGTTTAGATCACTCGGCCCCCCTCG







TGGTAGGCCACCGCTACTTTTGCACCTCTCCCGGGCCCTTAAGTC







CAGGTCCTCCCTCTTCGGGCTCCAGGTGCTGCCACCCGACTCGGG







TGCTATTGTGGCAAAGTGTTCGGCCTTCCTTTCCCAGGGCCCTTC







CCTGATTTCCTCTTCTCCCTTCATTTCTCAGCCAGGCCTCATTAA







AATGGCCTCTCCCTTCTGGGCTTCTGAGACCCGGGTGGGTGAAGT







TTGGATGGGACGGGAAGCAGAGGTGGCCAGGGACAGCTTCTTTCT







GATTCCTGATTTCTCTGGGTCTTCCACCTGTCCCAAGATGACTTC







TCCCAAGTCCTCTAATTACCCCGGAGCTTCCTGGTTTTAGCTGTT







CCAGGACCCACCTATAAACTGAAAATGCCAACGTACGCGCGATGT







GAGGGGTGCTGGGTAGGTTTTGGTTAATGAATCTGCATGTGTATG







TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ETF1. In some embodiments, the sequences from gene ETF1 comprise or consist of:











(SEQ ID NO: 922)



GAGAAGTCGCGGCGGACGCCCAGCTTCTCCTCACTCGCACCGGCC







CCCTCCCTTCCGCCTGCCGCCTTCCACTCCGCCCCTGGCGGAGGA







AGTGATGTCACAGGCCCCATGTGAGCGGATTGCAACACATGCAGC







TGCCTGGAGAGAGGGAGCCGGTGTCCTACGTCAGAGCCGCCGCCG







CCGCGGAGCCGCCGCCGGGGAGGAGCAGCCGCTGCCGCCCAGGAC







TGGGCCCTTAGGTAAGGCACAGGGAGGGAAAGGCCGGTGACCTTT







GTGGCGAGCTGCACCGGACGGGAGGAGAGGGCCTCTGGGGGATGG







CGAGGAAGGGGGCCGCTCTGCCCCAGCGGCGGCGCTAACTCTGTG







GTCTTGGTGTCTCCGGGCCCCGCCGGGCCGCTCTAGGGAGGAGGA







GGCGAGAAGATGGCGGACGACCCCAGTGCTGCCGACAGGAACGTG







GAGATCTGGAAGATCAAGAAGCTCATTAAGAGCTTGGAGGCGGCC







CGCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene RNF14. In some embodiments, the sequences from gene RNF14 comprise or consist of:











(SEQ ID NO: 923)



CATTGCTGTCTTTTATTACATCTTTTTATATTAAAAATAAATAAA







AATGTCATAAATAGTAATTGTGTAAATATAAAAGGCCTTATTAAA







AAAACAAATCTTAGTGCCTCAGTTTTCTCTTCTGTCAAGTGGGCA







CATAAAATTACCTAAGTCATAGGGTCCTGGGCATTTAAATGAGAG







AATGTACGTGAAATATTTAGCACAGTGGAGGGCTAGGCACACGGG







GAGACTGCAGTTTAAAAGACTAACCAGGCACATGAGACCAAAGAC







ACAAAAGTCTGTTATTTTCCTAGAGTCAAAAACAAAACTTATTAG







AAGGCTGTCTTTACCCAATCTTGTCCTCAAAATTGACTCTAAATC







TTTAGTTCACTTTAAACATTCAGTAAATTATTAACACTGCTTTGA







AATTCTCTTTCTATTCTCTCTATCCTGTGACCTCTGACATGATGT







CACAATTCACCTTCTGCTCTGACTTTGTGAGGATTTCCCAAGCTG







GTTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene RNF14. In some embodiments, the sequences from gene RNF14 comprise or consist of:











(SEQ ID NO: 924)



TAGGGTTGCTGTGTGTTTTGCTCTTGGTCTAATTCCTTTGGCTAA







ACATGAATAATTATATTAATGAATGGCTTTGGAGATTCTTCTTCC







GTCCTTTGCATTTTTCCGAGATATGTTTCTGGTTTTGGTATCTAT







TAGAAGGGTATTTTGGTTTCTGTTTTGTGACAATCAAGATTTATA







GTACAGAAAATTAGAAGTGATTATTTCTATTTCAAGGTTCTTTCT







GTGGCAGCCAGGTAATCCCTTGGGTGCCCTGATTTACTCACTTGG







TTTTTTTCTCTCTCCTGGGTATAAGACCAACTACATAATTTACAA







AGCCCAGGGCAAAATAAAAATGCAGCCTCTCATTCAGAAATTATT







AAGAACTTCAAGAGGGTGAGAGCACAGCATCAAATCAAGAGCAGG







GCACTTCTTTTTTATTTTAATTTTTCCATAAGTTATTGGGGTACA







GGTGGTATTTGATTACATGAGTAAGTTCTTTAATGGTGATTTGTG







AGAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene NR3C1. In some embodiments, the sequences from gene NR3C1 comprise or consist of:











(SEQ ID NO: 925)



GTAGGTTATATTTTTATTCCCATTCTATAGCTGGGTAAAGTGAGT







TCTAACAAAGTTACTTGTTAAAGGTCACTCAGAGGTCAGAGCATC







AGAAAAAAAGACAATCACAAGGCTGATGTTGTGTGCTGGATAGTT







TAAACTGAACAGGAAGAAAACATTTTTGTGGGCTTTATCTAAAAA







GAAATAGTTTGCTCTGAGTTTCTCAGTTTCATTTATTCAGCAAGT







ATGTGCCAAGTGCTATTGTAGGCAATGAAGATACAGCAGGGAACA







AAACAAAACTGACATGGAGCTTCCAACTAGAAAGGAGAACGAGAC







AACGCGTTTTAAAATATATATAATGTGTTAGGTAAAAAGTGCTAT







AAGGAGAGCTAAAACACAATGAGAGGCTAGAAGAGTGATAGTGGC







GGAGGGGCTTCTCTGAGGAGATGACATTTGATCAGGGGTCTGAAT







AAAGTAAAGAGGCCAGTTATGTGAATATCTGGAAGACTCAAGGGG







CAGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene NR3C1. In some embodiments, the sequences from gene NR3C1 comprise or consist of:











(SEQ ID NO: 926)



TAACTATAAATTGTGCATTCTTGCATAGATCCTCCTTGGGAATGA







GAAATTAGGAAAATCCAGTTGTTAAAATGAATGCCTAAAATCAAA







ATAAAATTTGTTTTTCTGGCACCTGCTTGATGACACAGACTAATA







ACCAATGACAAAATTGCCCTTGAACCCAAGTTTTCATTTCCTCCT







ATTGTGTGGTCAGGTTATGTAAGGGTTTGCTTTCACCCCATTCAA







AAGGTACCTCTTCCTCTTCTCTTGCTCCCTCTCGCCCTCATTCTT







GTGCCTATGCAGACATTTGAGTAGAGGCGAATCACTTTCACTTCT







GCTGGGGAAATTGCAACACGCTTCTTTAAATGGCAGAGAGAAGGA







GAAAACTTAGATCTTCTGATACCAAATCACTGGACCTTAGAAGGT







CAGAAATCTTTCAAGCCCTGCAGGACCGTAAAATGCGCATGTGTC







CAACGGAAGCACTGGGGCATGAGTGGGGAAGGAATAGAAACAGAA







AGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ECI2. In some embodiments, the sequences from gene ECI2 comprise or consist of:











(SEQ ID NO: 927)



GTAGTGCTAAAAAGTGACGACCTCACGCGCGCCCTCCTGCACCGC







TGGGCCCGGAGTGGTCAGGATGGGCGCGTCGGGAGGTCAGGGCTA







TGGTTCCCTGTCAGCAGTCCCCAAGGTCTCCGCCTGCCTCCGCGC







ACGCTCCCATCCTCCTGGGGCCGGACTCCGGGCGGGTGAGCCGCC







CCAGCTGAGCACCCGGGCCTGCACTTCCCAGCCGGGCTGCTTCTG







GGGTGCAAAGCGTCGCTTGCTAAAAGCGAGGTAAACGCAAGCCGT







TATCACTCGTGTGGACTTAACACTCTGCTTCTTGGAGAACACGTA







TGCTATAAAGTGAAATGGCCGGGTACAGAGTAACGGCTAAGAAGC







ACGCTGCACCTCCCTGGGGTCCCGGGAACCCGCACGAATCTGTGC







ATAGGGAACAAAACCGAAACACGGTGGTGGCCTTGAAGTGGTAGA







ATTATGAGTGATTTTCCCGACTTTATGTATTTTCTTAAATGGTCG







TGTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ECI2. In some embodiments, the sequences from gene ECI2 comprise or consist of:











(SEQ ID NO: 928)



GGCCCCGCCCCCGCATCTGAGCCCCGACTCCTAACCGCCTAGAGC







TCCCGCCCCGCCCACGCACCGACCCCCCCGGCCCCACCCCCGAGC







CCCCGCAGCCCCAGAGGAGGCCACGGGCTAGCGATGGCGAATGCG







GCGTGGCTACGCTCTCAGCCTGGCCCCGCCCCAACCCTGGCCCCG







CCCATGCCCCAGGCTCCTGCCTGGGCTCTGCTCTCGGCCCCGCCC







CGCACCCAAGCCCCGGCCCCACCCCCGAGCCCCCGCAGCCCGGGA







GCCTAGGCCCCGCCCCCGAGTCCCGGCTCCACCCCCGAGACCCGG







CCCCACCCCCGAGCCCCCGCAGACCGAGCTAGGCCCCGCCCCCGA







GACCCGGCCCCACCCCCGAGCCCCCGCAGCCCTAGAGCCGCCCAA







GGGATGGCGATGGCGTACTTGGCTTGGAGACTGGCGCGGCGTTCG







TGTCCGAGGTAAGCTTGGAGTCCCGGCCGTTCGGCCCATGGTCGC







CCGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ALDH5A1. In some embodiments, the sequences from gene ALDH5A1 comprise or consist of:











(SEQ ID NO: 929)



TGTTCTTTGTGCTTCTTGTATTTGGATGTCTAGGTCTCTAGCAAG







GCTGGAGAAGTTTTCCTCGATTATTCCCCCAAATATGTTTTCCAA







ACTTTTAGAATTCTCTTCTTCCTCAGGAACACGTATTATTCTTAG







GTTTGGTTGTTTAACATAATCCCAGACTTCTTGGAAGCTTTGTTC







ATATTTTCTTATGCTTTTTTCTTTGTCTTTGTTGATTGGGTTAAT







TTGAAAACCTTGTCTTTGAGCTTTGACGTTCTTTCTTCTGCTTGT







TCAATTCTATTGCTGAGGCTTTCCAGAACATTTTGCATTTCTATA







AGTGCACCCATTGTTTCCTGGAGTTTTGATTGTTTTTTATTTATG







CTATCTATTTCATTGAAAACTTCTCCCCTTATTTCTTGTATCTTT







TTTTTTTTTTTTGTGACTTCCTTAAATTGGGCTTCGCCTTTCTTT







GTTGCCTCCCTGATTAGCTTAATAAATAACTATCTGAATTCTTTT







TCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ALDH5A1. In some embodiments, the sequences from gene ALDH5A1 comprise or consist of:











(SEQ ID NO: 930)



TGAGCTCATGTGATTTTTGGGGGGTGTTAAAGAATCTTGTTTTTT







CATATTATCAGAGTTGGCTTTCTGGTTTCTTCTTATTGGGGTAGG







CTCTGTCAGAGGGAAGGTCTAGGGCTGAAGGCTGTTGTTCAGATT







CTTTTGTCCCACGGGATGTTCCCTTGATGTAGTAGCTCCCCCTTT







TCCTAGGGATGTGGCTTCCTGAGAGCCGAGCTGTAGTGATTGTTA







TCTGTCTTCTGGATCTAGCCACCCAGCAAGTCTACCAGGCTCCAG







GCTGCTACTGAGGGTTGTCCGCACAGAGTCCTGTGATGTGAACCA







TCTGTGGGTCTCTCAGCCGTAGATACCAGCACAGTATTTGGGGTG







TCTCCCAGGTCCTGCAGGAGCAATCTGCTTCCTTCACAGGATCTG







TATGCTCTCCCAGTTTTCCTGATTTATTCCTGCAGTCGTTCTGGA







GCAAAAGTTCACAATGCAAGCCTCCACATGCTGTTTTGTCCATCC







GAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene APOM. In some embodiments, the sequences from gene APOM comprise or consist of:











(SEQ ID NO: 931)



CAATCCACCGTGCCCCAGCACCTGCATCATACCTGTTCTAAAGCA







TTTACCAAGTTGTATTGCAATGGTTTGTCTACACAGCTAGTTTTC







CCTCTAACGACTTCTTCAAGATCAGGGGCTATGTCTTATTCGTTT







TTTTATGTCCCCGGGGATTAGCTAGTTCTTGGGAAACAACTGGGA







CTTGGGATTCAAAACAGTTTGCTAAGTGAATGAATGAGAGGCCCA







GTCAAGCTACTTCCCTTCAGTGCTGCACAGTGCAACAAATAACCA







GCTCAGATACAGGTTCAAAGACCACAGGCTTCTCCCTGGACAGCT







CAGCTCTCCTCATAACTCCTGAGAAACCCTGGACATGCCAGGGTG







TACTATGGAGTGGTTGCATCCGGAAGAGGGGGAGGAAGTCCCAAA







CACTAAGTAATCCAGGTTTGGGTTGGAAAACAAGGTTGAAGTTAC







TCATTAGCAGGTGAAAGGGTCAAGGGTCGAACGCAAGGGAGCTGA







AAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene APOM. In some embodiments, the sequences from gene APOM comprise or consist of:











(SEQ ID NO: 932)



TATGGACTGAGATTGGGGGAAGCCTATGGTGGAGGCTCTGAGGGA







CTTGGGTGGATGGCCTAGGATGACTGGAGACCATCTTGGGAAAGG







AAGAGAGGAAGGGGGTGTGAGTGTTGTGATAATGAAAGCAAGAAG







AAAAATATCAGTACTGTGGCCATCAATGCAGAGGCATGGCAGAAT







TGGGGGGTGGGGTGGTTACCCAGGTTGACTGGGGAGGGGCAAAGA







GGAAAAGTCATTTAATGACTCTTTGTCATGGATCCAATCCCCAGT







TGGAAAGAGGAAGGCAGCCAACACCTCTACCCCTAAATCTTGCTG







TTTTGACTGATGAAGAGGTTGAACCCATCCTGTGCTGGAACCCAC







CCTCTTTTGCTCCCTTCATTGTCTCTCCAGTTCCCAGAGGTCCAC







TTGGGCCAGTGGTACTTTATCGCAGGGGCAGCTCCCACCAAGGAG







GAGTTGGCAACTTTTGACCCTGTGGACAACATTGTCTTCAATATG







GCTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene FKBP5. In some embodiments, the sequences from gene FKBP5 comprise or consist of:











(SEQ ID NO: 933)



GTAGGGACCCCATGGAGTACGTGACGCCTGGCCCTCCAGCTGCAT







GCTCCAGTTATCAGGACAGAGACCCCTCCCCACCCATTCCCAGGC







TTCCCTGGCTCCCTGCTCTGCTGCTGGGAGGCCCTGTCACCACAC







ATGCGCGTGCACGCACTAACATACACACACCCCTCTCAGGCACCC







CCGGAAGACCAGCTGCAGCTGGGGGTGGGGGTGCACGGAGCACAG







CCAGGGTTCTCAGATCCCTAGCCTGCTCTCCCACCCCGCCCTAGC







CCCCAACTGCCGAGCTGCTTCTAGTTTTAAAGCAAAACATGACTC







TGTCCTTCCTGCTGCTCAGTGGCAGCAACACTGCCTGGGGTTATT







TTTAGCAGGGATGCAGGGAAGGCACAGTTCTCCTTGACTTTAACT







AGGGACAACCCCCACCCCTCAGCCAAGACTCACTGGAGGAGGCCG







GGTACGGAAACCCCCCTCCCAGAAAGGGGAAATGGTGTGTTTTCT







GTTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene FKBP5. In some embodiments, the sequences from gene FKBP5 comprise or consist of:











(SEQ ID NO: 934)



GGACAGTCATCTTGGACATGCTCTGTGTTCCATATCATGACTTCA







AGCCTTGCCTTTTTCATGTACTACCAGTGGGATCCTAGACTAGCA







CTTTACCACTTTAAGCCTCAGTTTCCTAATTTGTAAATAAGGAGT







AATTGTAATCATTCCAATATCACAGGCTTGCTGGGAGGATTAAAT







GAGATCATCCACATCAGGGTCAGCACAGTGCCCGGAATGGGGTAC







CTGTGAAATTAATGGTGATTCTTCTTTATTGTTACCTTGGAAAAT







GGAGGCCAACTGCAGGATGATCGTGGCTCCAAGGAGCTGGGGACC







CAAGACATGCACAGAGAAAGAGAGACCAACAATGTGAGGCCTCTG







ATGCATGAGACAGGCAGGGGAGTAGTGGGAGGAGAGAGCAAGAGT







GAGCAGCCAGGAGGGCCTCTTCCCTTCCCTTTACTCAGCCTGTCA







CTCCTCTGCTAGGATCCTGCATTGACACATCTTGTACTTCCTGTT







TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SLC22A7. In some embodiments, the sequences from gene SLC22A7 comprise or consist of:











(SEQ ID NO: 935)



AGGTGTGTCTTAGGCCAGGGCATCAGCTCCCAGCAGGGGAGAGGG







ATGCGAGGAGGCCTCATGGCTCAGCAGAAAGAAACCTGGTTAGGA







TCCAGCTTTCAGTCCTGAATCCTGGGCTGTCGGCCAGGCCATGGC







TGTAGCTGCAAATAGGCAAGGCTGACCTAAGGTCTCACAGGACAA







AGGGCACCCTGGGAGAGCTGGCCCCAGGCCCCAGGGGCCTCCTCT







TCTTAGGGATCCTGGGAGCTCTGCTGACCAAGGGGCTAGGGGCTC







ACCTCCTATGCCATCACCCAGCTACCTCATCATGGTAGCCTTGGT







CCCTGAACACTAATGGGTAAGCCCCAGAGCCACGAGCCAGTGACC







TCTGAATGGATGGCTTCCCCTTCCCCCAGGCACCAGGCAAGACAG







GTAGAGGTCCCTGGAGACCTGATGGCTGGGGAGGGCCCAGTCCTG







GGCCAGCCCCTGAGGGCTGGATTCTGGCTGCAGGCTCTCCAGTCC







ATCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SLC22A7. In some embodiments, the sequences from gene SLC22A7 comprise or consist of:











(SEQ ID NO: 936)



TACTTAAGCCCAGAAGTTGAGAGACATGTGAGAGGGATGGGCCTG







CCATTGCCTTGGGTGGTTACTGTGTAGGCATTAGATGTATTACTT







TACCTCTTAAAGTCTCAGTTTACCAATCTGTAGATGGGGGCTATG







AATATAAGGCACTTTTAGTTCATGGCTTGTAGTAACAGGATCCTA







CACAAGAACTGTTATCATCATCATTAATTGGGAGGTGATTAAGGC







AGGGAAGGGCTAAAGTGGCTTCAGGGAGTTGAGACAAAGAGCTGA







GAAGGCAATTTCTGCAGCAAGAGGTGGAGAAACAATAGAGGCCTT







CTTTTCTCCCTTCCTCTTTTCCAGTCCCAGGTCGGTATTTACATA







ATCCATCTGGAGGTGGAATGTCGGTGGAGGCAGTCTCCCTGGGAG







GCAGCAGGTCGAGGCCTTCCTTGGGAAGAAGCTGAGGCTGCAGGA







CTGGGGAGGGACAAAGTTTCCTATTCCCCAAGCTGGCGTGAATCG







TTGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MLIP. In some embodiments, the sequences from gene MLIP comprise or consist of:











(SEQ ID NO: 937)



TCACTACAGATGCTCCTGTATTGTTGCTTACAATTTATATACTAA






GGATAAAGTTACTGATATTTTGAAATAACTGTGTGTTTTCTAGTT






CTTTATTTGGACACAGTGTTTGCATGTATTTATACCTCTAAATGT






TCTCTATGAGCATGACCAACTGATTATTGAGATTGGCAGGTGCTA






GCGCCCTTCAATCAGCTTCTCATTGCTGCACAATCTGGCTTTTCT






CTTCTTTTCACAGAATATATAATTGGAATACATGCATGAAAAGAA






AAGAGGGAAAACACATATCAGTGGCTTTTTTTCTTTTTCTTTTTC






TTTTTTTCACTTCTACTGATTCCATTTCAGCAGTTAGTATGACCT






CTCATAATGTTCCAAATCTAAAAATTGTCAAAACAGAAATCTACT






CTTCGGAGCTGACACAATTCCTCAATTTTGGAATGACTCTGCTTT






ACCTCTTTTTAGAATTTCTTCTTTCTGCGTGAGTGTGTGTGTGTT






GTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MLIP. In some embodiments, the sequences from gene MLIP comprise or consist of:











(SEQ ID NO: 938)



TAAAAGGAAGTCTTTGCTTAATGCTTTCAGTAACTTTTAAAAGCA






GTGTACGGTGCTGGTGGTATTTGCTGCAAGGATGTGAGGGAGAGC






TGCCCAGTGAAGATAAATTTGTATGTATATTGAAATGAAATGCTA






TCTTCACTTAGAGAAAAGGGTGAGCTGATAAAGACAAAACTGCTC






ATTGGGCAAAGAAAATGCTCAGGATTGCCCTTGGCACAGTGATAA






TGTAAGATACCCTGTAATGCCTTAACAGGAGGCTAGAGGCCATCT






GCGAGTGTGGGTGTCAACATCTGAAGGAAGAGAACTTTTAGGGAA






ATATAAGAAAGGAAGGCATTACAGCCCAAAACTGAGCAAAGGAGC






TGTTAGCCTGCGTGGCATGCGGGAAAACAGTTTCTCAAATTATTA






GGCTATCCGGTGAGAAGATGACGTTCTTAGAGATTTTGAGCATTT






CAAAGTGTATTTAGAAAAAGATTTTAATCCTTTTATCTCATATTT






CTAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ENPP1. In some embodiments, the sequences from gene ENPP1 comprise or consist of:











(SEQ ID NO: 939)



TAGGAGCAGCGTGCCTATGGAAAATAGAAAATAGGTTCTTTTTGT






TTTTGTTCTTGTTTTTTGCTTCAGAAATAATTATAGAAATCTGTG






TTTAGAAGCAGTCCTAAGAAGTTACATGCAAAGAAGTACCAGTCT






TGGAATGAGAGTGAATCATACACATACACTCCCAGTTAATATCCT






AGGTGGTTTGGGGCAAATAACTGGGCTTTCACATTCGGTCTAAAA






ATGTGATAAGACATTCTTCTTTGCTCACATAATGATATTGTAATA






TATAAATAAGAAGAAACATTAACCAAAATTTGGGTGACGGAAAAG






ATTTTGAAAAAAGTGAAGTGATAGGTACAGCTGAAATTCTGTCTT






ACCTATCAGATCTTCAACTAATATGAGTGCTACACCCATGTTTAA






CGAATTTAACCTTGGAAGTGAAAGAAGTTCTGCTCTGCATATTAA






ATTTTTTGTTAAAGTTACAGCATGTTTTGGGATTTTTTTTTTCTC






CTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ENPP1. In some embodiments, the sequences from gene ENPP1 comprise or consist of:











(SEQ ID NO: 940)



TAAGTATATCTCTGTGATAACTTTGAATATGGTCATATTAAGAAT






ACCTTCCTTTAGGCCGGGCACAGTGGCTCATGCCTGTAATCGCAG






CACTTTGGGAGGCCAAAGTGGGTGGATCACCTGAGGTCAGGAGTT






CGAGACCAGCCTGGCCAACATGGTGAAACCCTAAAAATACATATA






CGATACTATACTATATCTATACTAAAAATACAAAAATTAGCCTGG






CTTGGTGGCAGCTGCCTGTAATCCCAGCTGCTCTGGAGGCTGAGG






TACAAGAATCGCTTGAACCCAGGAGGCGGAGGTTACAGTGAGCTA






AGATCATGCCACTGTACTCCAGCCTGGGCAACTGAGTGAGACTTG






GTCTAAAAAAAAAAAAAAAAAAAAAAGAATACCTTCCTTTAAATC






ATTATATCGCTAAACTAAAGGGAATCACAGTTAATTTTTTTATGA






TTAAATCTCCAATGCAGAGCTTTTGTACACAAATGGATTTTATGG






GACT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene VNN1. In some embodiments, the sequences from gene VNN1 comprise or consist of:











(SEQ ID NO: 941)



GTAAAGAAACAACTTGTGAAAAATTCACTAGTAAACATCAACTTG






ATTTACCTGGGAAAACTTTGTTGATGATCATTGCATAGATCCATG






ATCAATTCTTAAGTTTCAGTATAGCTTATTTTTCATCTACTATGG






GTATATTTACTGGGAGAGCAAATATGAATTATGAAGTCACAGAAG






TCAGAGCTAGAAAGTAGCTTAGAAATCATCACATTCAGTGTGAAC






ATCTCTGGTCTCTGACTCCTCACCAGTGAACAGAAAAATATTTCC






CTGTGTAGGTCTGTGATTTGAAAACTATATGAGTAAATGGCAAAA






GAGAGTCACATCAGTTTAAGATTAATAGTTTTCCTTTCTCATTGC






TAAGATAGCTGATGAGGTTAATGTAGTAAAGTCCTTAAAGTGTAA






GCTGATTGTAATCTAAGAGGTGATATGGCAGGATTTTAAGTGGTT






TAAGTCAGGTCTCGGCTACAGAGATATTAAGTGTGGTGAAAGCAG






CACT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene VNN1. In some embodiments, the sequences from gene VNN1 comprise or consist of:











(SEQ ID NO: 942)



CATCTCTACTAAAAATACAAAAATTAGCCAGGCGTGGTGGCAGAT






GCCTGTGATCCTAGCTACTTATGAGGCTGAGGCGGGAGAATCGCT






TGAACCCGGGAGGTGGAGATTGTGGTAAGCCGAGATCTAGCCATT






GCACTCCAGCCTGGGCAGCAGAGCGAAACTCCATCTGAAAAAAAA






AAAAAAGAGAGAAAAAAAAATGCTTGACTAGAAGCCCAAACCTCA






CCATTATGTAACATATCCATGCAACAAACCTGCATTTGTACCCTT






TGAATCTAAAATTAGAAATAAAGAAAAGAAAAGAAAAAGAAAAAG






AAGTGACAGTGCACTGAAAAAAAAGGAAATTAAAATGCTTTGGAA






AAGAAAATAAATTATAAAAATATAGAAAACAAAATAAGATTTAAG






GGGTGTGGGGGAAGCCCAAATAGTTGTTACTCAGCCACTCAGCTC






CTCAGCTCCTCTTGCAGGCCCCCCTTTGGATTAAGTTGCATTTTT






AACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SGK1. In some embodiments, the sequences from gene SGK1 comprise or consist of:











(SEQ ID NO: 943)



GTTCGTACTTACCGTACTATATTTTGCAGATGCCTCAAAGGATTT






GGGGTTACTTGGCATGGGGAAGGCACATAAGGTGGGGTGTAGGAG






AGGGTCTCTGGTTGTAGGTTTCTTAATTTAATGTTTGAAAACAAA






CATGCAAAAGTCTGTGTGCAGGTTGATGTTTCTGGGCAGCCTGAG






CAAAATTTGCTCTCTCAAGAGGGAAAGGAACCAGGTGGGAGCAGA






GCTAGGCTGGGCTAGGCTAGTTGAATGGTGGGACATGACATACGG






GTGGCACTGGCAATAACAAAGTCACATTCTATGAAGATTCCCTGC






AAGAGGAAGCAGACATGGGCCAGTTACTGTGATTTGAAATTGCCT






AAACATTGCTTTAGGTTGGCATGTCAATTTCAGGTACTAGTGTTT






TTTTTGTTTTTGTTTTTGTTTTGTTTTTGTTTGTTTGTTTGTTTT






GAGACGGAGTCTCGCTCTGTTGCCAGGCTGGAGTGCAGTGGCGTG






ATCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SGK1. In some embodiments, the sequences from gene SGK1 comprise or consist of:











(SEQ ID NO: 944)



AGTAGGTTGCTGGGACAGAACAGGGGGCGCCTGCTCCACCCATCA






CGTATCCGGTTGGAAGAAAAGCCAGTCTCAACAACTTGATTCTCT






CGGTTAAATGATAAGTTTAGCCGACCATCCGTCTCCAAGTGCTGA






ACATTAGGGTAGGGGAGGAAGGAGCACATCAGACGTTCCCTTTCT






TTTCCAACCACAAATCATACGGTTGCATCTATTCTCTATTCCGCG






TAGAACCCTCCGACCTGAATGCCACGTTCCTCCCGGAATGCATGC






CACAGAGCCTCTGAGAAAATAAATCCTGTGCATGTGTTTTGAATT






TTTTCCGGACTGATTTGTGAGGATAATCAGTTAAGTTACATTTCT






TGTGTGCGTGCTGGGGGTGTAATAAAGCTAGGACACTGATGACTG






TAATTTTCTGAGTGATGTCGCAATGGGGAGAATAAATGGCCCAGT






GTTGAACTTTGCAGCTTGCTCCCTTCCTGTACTTAAATTGCCTTG






ATAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PLG. In some embodiments, the sequences from gene PLG comprise or consist of:











(SEQ ID NO: 945)



ATATCCCCAGAGTGGGTGTTGACTGCTGCCCACTGCTTGGAGAAG






TATGTTTAGGGGACAATTGACATGAAGTCTTGTCTTAAATACTTT






TTCTGTCCTTCTTTTCCTCCTTTCCTCCTTTCCTTTCTCACTCTT






CCTCCCTTCCTTCTCTGGCTGTGACACTAGGGACCAGGCCAGGGC






AATTGGATAAGAGAGAAGGGAAGGGTTTCTAGAAAGAAACTGCAG






AGGAAAGACACAGTACAGATGATTTTGTGGGCCTGAATAAACTGC






AGAACAGAGCTGTTCACTACCATAGGCTGTATCAGTCTCTGCCCA






AACAGCCCAAGAACATTCCTTAACTGCCTGTTTCAAGCAAATCAT






GAATTTTGCTTCTTGCCACTCAGAAGTCACTAATTCTGAGTGGCC






AAGGGTGTCAGGGAGACAGCACCAATTTCATGGCACAGAGGTTAC






CTGAAGGGGCTGGACCATATTTTCCTCTTGACGTCCTCATCTTTT






CTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PLG. In some embodiments, the sequences from gene PLG comprise or consist of:











(SEQ ID NO: 946)



TACTCGTTCACCTGTGGTCTTCACCCCACGCTGGTGAAGATATTT






GCTTTATGTCTGGGTTTTATGGGCCATGGCCACTGCATGGCAGTG






GGGAGGAACTGTCTATCACATGAAAGGCTCAAGGGCTTTGGGGAC






AGCATCAATCTTCAACCCTAGCCCTGCCACATGCTAGCTGTGCTC






TTGAGAAAGGCAGCAGGACTCCGTTTTCTCATGTGGAAAAAGAGT






TGAAATGAGGTACTCTGTTACTCCTAGAACTCACTTAATGTTCAC






CAGTTCATACACATTCATGATCAGAGAACGATTCAGTTATTCCAG






GCTGACAATTCCCCCTTCATCATAATATGTTTAAGAGAATCATAT






AAGACTATATTTGTTTCAAAGCACTTTAAAAACCACAAGATCGAG






TTGGGTGTCTGGTGTGGGTGCCTGTAATCCCAGCTACTTGGGAGG






CTGAGGCAGGAGGGTCACTTGAGTCCCGGAGTTTGAGGCTGCAGT






GAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ABCB4. In some embodiments, the sequences from gene ABCB4 comprise or consist of:











(SEQ ID NO: 947)



GGTCTCGAATCCTGACCTTGTGATCCGCCTGCCTCGGCCTCCCAA






AGTGCTGGGATTACAGGCGTGAGCCACCTTGCCCGGCTGATAGTG






GTTATTTTGAAGTAATGGAATATTTTATTCTAAATTTTCTAAAAT






GATTATATATTTTATAAAGTAAAGCATTCAAAAGAAAAAAGCTGC






ACAAATTACATTTTTAGCACATTAGGAATGAAAAACTGGAAAATA






CTCAAAAGGGAACTAGTTAAACAGAAGATTAGAAAATTTAAATAC






TTATGAATTTTTTTACAGCATGGGAACCCATTTGTGTTATATTTA






AAAAGCAGAACATAACTATGTAGATTAAATGTTGATTATACAAAA






TACACATGCATAAAGTTCAAAGATTCTAAGATACTAGAAATTAAA






AGTTGATTAGAAAGGTAACATTTTCAAATTTACTTGAATGTTAAA






ACTAATTTATACAATTTTTGGGATAAGGTGTCTGTCTGATTTATC






AATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene GSAP. In some embodiments, the sequences from gene GSAP comprise or consist of:











(SEQ ID NO: 948)



GTACGCAAGTATTTCTCACTTTTATCATGGTGTACAAAGAGTGGA






AACAACTCTGTTGTAGCAAAACCCTTTTGAAATGCACTAGTTCAA






ATCAGTTCTCACCAGCTTCAACACCATCTAGCTTAATTCTATACT






CAGCATTTAAAGTTATGCCCCCCTGCATATGTACCCTTTAATGTA






TAATAAAAGCTAAAATTAAAACAAAGTTATGCCCGTTACTTTACC






CATCCTCTTTCCCTGGCACATGGTTGGTGACAGGGGAGGGACAAA






TGAGCCCAGTACTGCCAAGAAGGGGAATTTCACTTAATACAATGT






GTAACATATGTTCCTACTCTGATTAAATTCTAGGCATTAGGCCGA






TGGTCACATTCATATTTATGGGGCTTTCTACAAGGCATAGCATTT






CATGATTAGCATGAACCCTTGTGTAGTACTCAAAGCCACTGCTTA






GGACAAATGGAGCATCACAGATGAGCTCCTTGGCTGACATGCTGC






TTTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene GSAP. In some embodiments, the sequences from gene GSAP comprise or consist of:











(SEQ ID NO: 949)



TGAGGACACTGCAATTTGGAGAGGGTGGGTAACTTGTGTAGGGTA






ACACAACTAGTAATGTATAGAATCTGGACTTGATACCAGTCTTAC






TTCACAGCTCATGATCATGACCTCCATTCTGTGTCTCTTTTCTTC






TGACTGTCCACTGTTGGCTCTTGCGTTGTGACAGGCCTGGAGCAA






ACCCTGTAGCACTTTACCTTCACTGATGGATGGGGGTCTGTAAGT






GTGAGTGGTGGGGCCAGTTAGCTGGGCTGGGGAGGCTGAGCTGGG






GCACTGCCCACACAAACAGAGGATGCTTTGTTAGTTTCTGGTGCA






CATTTTCAGTCTTTCTATTTTGGGTTCAGTGCTTCAGAATTGACC






TCAGAAAGGCTAGTTGTGTGTCTGGATGCAGCCTGAATGCCAGCT






GCCCTGAAATGCTTATCTGAGTTGTGGAGGCCTAGCTGTGGGGCC






CTTGAGGGAAGGAATGGAGCAATGGTTAATGGACTGTCTTCACCC






TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ABCB4. In some embodiments, the sequences from gene ABCB4 comprise or consist of:











(SEQ ID NO: 950)



ATCTGACTTGTAAAATAAGTTTCTGGTTTGTCATCATAAAACATA






GGTATGTCTGTCTCATTCTAGATCAATTTAGAAGACCCAATTTGG






GGTTTATTACGGCATTTGAAATTTGAGAGTTAGAAGTGACACCTG






TCTTGATGAAGTATTGAAACAACAGGCTAGCTCTCCCTAAGTTAT






TAGAAACATCCAGAAAAAGAACAAAATTCAAGGTAAGTACAGGTG






TAGAAATATAGAAGCCACAGACATAGGGAAATGGTCTTATGGTCC






TGAATGGGTTGGGTAGACAGGAAAGTTTGGGAATAATTTTCCCAA






GTACAAAAGAAAAATAAATTGTTCAATAGCCTCATCTCTCTTGGT






AATTTTCTAAATCATTCTATTGTGGAATCAAAGTTTTTTAAATGA






ATGAATTTCCAGCTTTATCATCCAACATCAAGAGAAAATGAAATT






GCCCTACCGAGCTAACTCTGAAAGCACATATCGGTTCCATAAAGC






AAGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene STEAP1. In some embodiments, the sequences from gene STEAP1 comprise or consist of:











(SEQ ID NO: 951)



AAACTCTGTGTTGAAAAAGTCATCTATTTTAAAATTTTTATTATA






ATTACACTAGGGCTTGGTTGTCAGTCGGTTTTATATTGAGAAATG






TTTTTTAGGCTGAAAATAAGGTACAACTTTAGCAACATAAGAAAC






ATTTTGTTCATGGACGAGGCCATATTTCAAACTTCTACCACCCTC






ACAAGATTACTTTTTAATATTGTTGTTCTTCTCCTTGTTTACCAA






TATGCATGCACATTACAGTTCTATCCAAGTAAACCATCAAATAAT






TATTAGAAAATTAATAGATTATGGAAGTGTTGCATTCAAGAAAAA






AAGTGATAGCTCCTATGTCATTTGTCTTAGAGGAATGCAAAAAAA






AAAAAAGAAAAAGTCATAAATTTGTGGAGACCTGTTATCAGGGCT






TCATAGTAGGCACAGGGAAGAGTGTAGAAGGAGATTCACATTCAG






GAAATAACTGTTGTTTGCATTTCTTCTTTCTTTATTTACCTTCTG






GTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene STEAP1. In some embodiments, the sequences from gene STEAP1 comprise or consist of:









(SEQ ID NO: 952)


TAAATAATATATAAAATAACCCTAAGAGGTAAATCTTCTTTTTGTGTTT





ATGATATAGAATATGTTGACTTTACCCCATAAAAAATAACAAATGTTTT





TCAACAGCAAAGATCTTATACTTGTTCCAATTAATAATGTGCTCTCCTG





TTGTTTTCCCTATTGCTTCTAATTAGGACAAGTGTTTCCTAGACATAAA





TAAAAGGCATTAAAATATTCTTTGTTTTTTTTTTTTGTTTGTTTGTTTT





TTGTTTGTTTGTTTGTTTTTTTGAGATGAAGTCTCGCTCTGTTGCCCAT





GCTGGAGTACAGTGGCACGATCTCGGCTCACTGCAACCTGCGCCTCCTG





GGTTCAGGCGATTCTCTTGCCTCAGCCTCCTGAGTAGCTGGGATTACAG





GCACCCATCACCATGTCCAGCTAATTTTTGTATTTTTAGTAGAGACAGG





GTTTTCCCATGTTGGCCAGGCTGGTCTCGATCTCCTGACCTCAAATGAT





CCGCCCACC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PON1. In some embodiments, the sequences from gene PON1 comprise or consist of:









(SEQ ID NO: 953)


GTAACAATATTTACTATTTATTAAAACCAAAACCAAACTTAAAACAAAA





ACAGAAAAACATGCCAATAAATGAATTATTTTTTCTCATTCAAACAAAG





CATAGTCATGAATAATGTTCTTACTCTGGGTTTTAAACTTACCCTCTAT





GTTAATTGAACTTTATAGTAAAACCACTTAAATACTTGAGAATGCTAAA





ATATTTAAGATTTGTAAAGCATCTAATTTTGTAAAATACTAACATTCAC





ATTATCCCTATAATTACCATAATAATAATATCCAGCCCTGATCATTATA





GCTTTTCCATTTTAAACGATTTTCAAGACTCGGGCATTTACAGGAACAG





ATTTTTCTAACCCAAGGCATCTGTAAGCTATAGGTCCAGTGACAGAAAG





TCACCATGGTCAGCTTTCTGCCGGTCCCATTATCTTCAATGCCTCCTGT





CCGCTGGTTCCTCTGTTCTGTGTTCAAAATGCTCCTCTGCATGTTGGTC





CCTTTCAAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PON1. In some embodiments, the sequences from gene PON1 comprise or consist of:









(SEQ ID NO: 954)


TCTTATATGGGAACACAATATATAAAACAACAGCTTTATTGTTTTGATT





GAAGGCTAAGAAAGGAGTAGTTCCTCTGGCCCACCAAAACTCTGTCTTT





GAGATGCTTCCAAGGACCTACCAGGGAACAGTTTAAAAGCCATAAGCAA





TATTCTCTCTGTATTCCTTGCTTATATCATTGAAGACTATTATTAATTG





CCTTTTTGGTCTGCTCTTCCATTTTTGATCTTTCTCATGGGCTGGAAGC





TGTAGTGTCGTGCACATATGACTTTGCTGCAGGGGATGAAAGGGTTTGT





GTGGGGTGGCCTAGTGGAGCATGTGGCTTCCAGAAGACTTCTCCTGGGG





ACCCATTACAGAAGCTAACTAAGAGGCTTCCTATGTGGCCATCTGGCTA





GCCCTGTGGTTTGGAGCAGTCAACATGAACCCATGGGCCATGCAGCGCC





TCTATTAGCATTTGAAGTACTGGTATTGTGCTTGATTTTTCTCCTCCAT





GGTTTATAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene TFR2. In some embodiments, the sequences from gene TFR2 comprise or consist of:









(SEQ ID NO: 955)


GTGAGTGCGCGCGCGGGCGGGGGGCGCGCGGGGAGGGGGACGCGGCTCG





GGGCGCTCACCAGCCCCGCTCCCCAGGGCTCACCCCAACACCCTGCACT





GGGTCGATGAGGCCGGGAAGGTCGGAGAGCAGCTGCCGCTGGAGGACCC





TGACGTCTACTGCCCCTACAGCGCCATCGGCAACGTCACGGTGAGCACC





CCCTGCCCGCTCCTAGGATGGGGCGCGGCACGAGCCCTTTCCTGGGTCT





CGAAGGAGGGACGCGGGGAGCGCGCCTCACCGTCCTCGTGTCCCCAGGG





AGAGCTGGTGTACGCCCACTACGGGCGGCCCGAAGACCTGCAGGACCTG





CGGGCCAGGGGCGTGGATCCAGTGGGCCGCCTGCTGCTGGTGCGCGTGG





GGGTGATCAGCTTCGCCCAGAAGGTAAGTGTCCCTGGACAGAGGGAGGG





CTTGAGCCCGGGAACCGGAGGGTCGGACTGCCAGTGAGAATCGTTCAGG





GTGCTGTGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene TFR2. In some embodiments, the sequences from gene TFR2 comprise or consist of:









(SEQ ID NO: 956)


AGTCTCCGGCGCCGCCCTGGTGGTGATGGGAGAAAATGGGTCCGCGGAG





GGCCCTGCACTGCGGCTCGCGCCTGTAATGCCAGCACTTTGGGAGGCCG





AGGCGGGCAGATCACCTGAGATCAGGAGTTTGAGACCAACCTGGTCAAC





ATGGTGAAACCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGAT





GGCACGTGCCTGTAATCCCTGATACGAGGGAGGCCGAGGCAGGAAAATG





GCTTGAACCAGGAAGGCGGAAGTTGCAGAGAGCCGAGATTGGTCCACTG





CACTCCAGCCTGGGCGACAGAGCGACACTCCGTCTCAAAAAAAAAAAAA





AAAAAAAAAAAAAAAGCGTCCGCGGGGAGCGCTCTTTTCCTAAACTCAG





GAACCCCTCGCCGCCCCTGCCCCTGGCGACCCCACGTCTCTGGCATCCT





TCCCTCTTCCCTCCCTCTCCTCCGGGCGCCCAGAAAAGTCCCCACCTCT





CCCCGCTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SLC39A14. In some embodiments, the sequences from gene SLC39A14 comprise or consist of:









(SEQ ID NO: 957)


ATCTCGGCTCACTGCAACCTCCGCCTCCTGGGTTCAAGCGATTCTCCTG





CCTCAGCCTCCTGAGTAGCTGGGATTTCAGGCACGCGCCACCACGCCCA





GCTAATTTTTTGTATTTAGTAGAGATGGGGTTTCACCATGTTAGTCAGG





CCGGTCTCGAACTCCTGACCTCAGGTGATCCACCTTCCTCAGCCTCCCA





AAGTGCTGGGATTATAGGCTTGCGCCACCGCACCTGGCCTACTTGTATG





GTTTTTAGGCTTCCATTGCTGTTTGCATCCGTCTCTCCATGGAAAAGGT





GTTTCTCTTGGATAGCAATTTTGATAGTTTCCCTAGGCTTCTCCGCAGC





ATCTGGTGAGAGATGGACTCAGCAAATGTTTACTGATGGGTTGGTGGAT





CTCAGTCTGCCCCATCACACCTGAATTCAGAACAGGGCCGCCCCAGGCA





GCAGGAGTGTCCCCACCCTCAGTAATAGAGGCCCTCGTCTGTTCTTGTT





CCTCCACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SLC39A14. In some embodiments, the sequences from gene SLC39A14 comprise or consist of:









(SEQ ID NO: 958)


TGCAGTAGAACAGAAACTTGCGCGTCCTCTTTCCCTGGGGCGGCACAGC





TGAGCCAATCCCCTCCTCCCCTACCCTGGGCTCCAAGGCACCCAATGAC





TGCCACCTGAGAAATGAACCTGTCCCTCGTATCAAAGCCACAAACGACT





TCTAACTCTGGATCTCAGGGCCCACCTCTCACTCCTCTGGCCTCTGCAG





GTGCTGTCAGATGCAAGGTTCTTCCAATTTCCCTCCTCCTGGGGACTTG





ACAGTCTCCCCTCCCTTGTTAAGCCCCATACATAAGGCATCAAGGGAAA





GCAGCAGCTATCGACTGCCGTCCATGCCTTTGGTGCTAAGTACTTGCTC





TATTCTAAGGAGAAATCCTGGCAGTACTCAGGTCTGGGCACCAAGAGCT





TATCCATCTGGAATGAGATGGAAGGAAAAGAGGTTACCAAGTGATATGT





AGAATTGGCCTAAAAAGCGAGTGTTCTTTAATTTTGAGCAGGCCATCGT





TTCTAGCAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene RBPMS. In some embodiments, the sequences from gene RBPMS comprise or consist of:









(SEQ ID NO: 959)


CCTTAGGAGTCGCCCAGCAGCGAGACAGCTGTGGATCCCCTCCAGGCGC





ATGGGAGGCTCACAAGGCAAGGACTCTGATGGTTTTGGCTTCCACCTCC





TGTACGGTGGCCAGTGCTGTGAGGGTGGTTGGTCCCACAGGGAAACCTG





TGAACAGAGTCATGTCTGGGGCCCCAGGGACATGGCTTCAACCCCTCAC





CTGGCAGTCCTGCCTCCCAAAAGTAAGGCAGGAGTCCGGAGATTCCCAT





TGCTTGCTTCTGTGAATCTCAGGTCACTTTAGCACTTGACCCCTGACCC





CAGACTGCCTTGTCCTTGAGGCCTGGGATTCCTAAGGTATATACTGTCT





GCTCCAGAGCTGTGGCTGTGGCTATCCGGAAAACGACAGCTTTGAAGGA





CTGCAGAGCTTGCCTTTGGGGCTGTTCTGCCCAAGGCCTTCCATTGTTT





TCAGACATAAATGAAATTGTTAATCCTCTGACATTTACCTTTTCCTCTC





AACCTGCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene RBPMS. In some embodiments, the sequences from gene RBPMS comprise or consist of:









(SEQ ID NO: 960)


TGAGGCTTTCTAGGAGCACCTGGCTTAGCTCTCACAGGAGGAGGGGCGG





ACGGGGAGGCCAGGCCTCATGCTCACAGCGCCAGCCTCCTGTGAGAGTG





GGCTGGGTCTCCTTTCCTTTCCCAGGCCCTTCCTGGAGGCGATTTCTTT





CTTTCTTTCTTTCTTTCCTTTTCTTTTCTTTTCTTTTCTTCTCTCTCTC





TCTCTCTCTCTCTCCCCTCTCTCCTCTCTCTCTCTCTCTCTCTCTTTTC





TTTCTTTTCTTTCTTTTCTTTCTTCTTTTTTTTTTCTGAGGCAGAGTCT





CGCTCTGTCGCCCAGGCTGGAGTGCAGTGGTGTGATCTCGGCTCACTGC





AAGCTCCGCCTCCCGGGTTCATGCCATTCTCCTGCCTCAGCCTCCCAAG





TAGCTGGGACTACAGGCGCCTGTCACCACGCCTGGCTCATTTTTTGTAT





TTTTAGTAGAGACGGGGTTTCACTGTGTTAGCCAGGATGGTCTCGATCT





CATGACCTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene DPYS. In some embodiments, the sequences from gene DPYS comprise or consist of:









(SEQ ID NO: 961)


GTAAGTCTAAGTTCTTGTATTCCTTGGCTTCATTGTGGAGAAAATGCAC





TAACTTGCACAGAAATAATGCTTTGGCATTAATCAGCCGCTTCTCCTTA





GATGACTTCTTTAGCTGTGCTCAGGTATTTTGATCCTGTACACAAGATA





AAGGTTTTGATGTGCAAATATTCCTTTGTTTTTCTTGGCATAGAGGATG





TAGAAGTTGTGTTGCATTCTCTGACAATGGATCTGTATATAAAACCAAT





ATGAATTTACCACTTCAGTGCCTGGCAAATGCCCGAGTTCACATGGAAG





AAAGGTAAAGCGTGATGAAGGAAATAAAAGGCTTAGTTTGAAAAGGGTT





GAATTGAAAACTCAAAATAATTACAGCCTTTTTAAAGCTTTCAGATAGA





AAAATTCCAGCACAAAGGATGTCACTTTCAGTTAGCCAAATTTTCCAGT





TGAGTTCATCTTCAGCCTATCATTCCTAGTAGATTCCAGCTTTTTGTTT





CCAATTTCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene DPYS. In some embodiments, the sequences from gene DPYS comprise or consist of:









(SEQ ID NO: 962)


GCACCACTGCACTCCAGCCTGGGCAACGAGAGTGAAATTCCATCTCAAA





AAAAAAAAAAAAAAGTGTTGTCACCTATTCTCACTCTATAATATTCTAT





CTAATGTCTACATGATTATCTCATAAATCTTAGGTCTGTAATAAATAAG





TCAGATCATAAATGTGGTATTGTCAAACTTTAAAGGAGAATCGAAAATA





CCATTACCAAAATTTAGCTGTCACCTATAACAATTTTTGTGATTCTAGG





ATGAATGGGAAGAATTAGTGTGGATGCTAAGTAGTGGGGTTTCTGAAAA





GTCATCTTCAGGCTTCCATATGTTACCCCATCATCTCCATCATTGGCAT





TATGACTGTTTAATACTTACCAGCATCCTCAGATGCTCTACAATATAAG





AAATTAGGTGCAGTTCTGTTTGTTTAAGAGATGCAAGTCTTGTCATTTT





ATCGATAGGATACTTTTTAGTATTTGATCATTAGAATGATTCTTTGAAT





TGCTTTACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene RCL1. In some embodiments, the sequences from gene RCL1 comprise or consist of:









(SEQ ID NO: 963)


TATTGGGATGGGTGAGAGGAGGAAGATGCTTTTTGGGAGTCAGATCTGT





TTAATTTTGAGCAAGTGTCTTAGTTTTTTTGTCTTAGTTGTCTTTAATG





TAAGTCCTAGAACTCGGAGCCATGGCCCTCAACACAGGGTTACTGTGAG





CATAAAGTAAGGTGATAATAGATGTGAAAGAACTTTGGGAGCCGTGAGC





TCTGTGGGATTATGTGCTAGGATGGTGACATCTCTTGTGCTTTCCTGGG





GACAGTGGGCAGCCTCAGACCTCAGAAGGACAGCACCTGCTTGGTCCAA





AGGGTATGAGATTCAGGACTGTTTGTCTAAGCTGTCTGTGGTCAAAAGT





ACATTCTGGACTGAAGAGATGGTGATGTAACTGGGCTGTGTGTGTATTG





AAAAATCCCTCTTTGCTCTTTCCTCATTTCAGGTTTGTCCTTTGAGTCA





AGTTAAATATTTATTGAGCATTTCTGAGTACAAAAGTGGTGTATTGGAG





AGTTGGGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene RCL1. In some embodiments, the sequences from gene RCL1 comprise or consist of:









(SEQ ID NO: 964)


TAAGTTCAAGTGGAGCTAACACTTGGGTGCCGGCTGGAGGGTTTCAGGA





GAGAGATTGGGACCTCGGGCTGGGAGAATGGGGCAAAGCAGCTCAGGTT





GAGGAATTTGGTCAGTTTGGATGGGTGGAAGGAGTTGATGGTGCAGAGC





TGAAGATGGACACAACTGAGCAGGCTGGGGGCTCTAACAACAAGATATG





GGAGGACCAGGAGCCACCGAGAGACTGTCACCAGCATGAGGAAGATTGG





TCTTTTAGTATGAAGTTCAGGATGGGTCAAAAAAGGGAGCTAGATACCA





GATCAGGTCATGTATCGTCTCTGAGCACTTTGTTTCTGCAGTTCAGATG





AAAATTAAATATGTTATTGTCAGCAGTTCTTGAACATTGGGCTGGATTG





GGCAGATTATATACCTCGTTCTACCTGTTGGACTTGTCACGTGGGGTTT





GTATCAGGGTAGTGGGAAGGTCACATGTTATTTCTCCATTTTTTTAGTT





CCAAATATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene TOMM5. In some embodiments, the sequences from gene TOMM5 comprise or consist of:









(SEQ ID NO: 965)


GTAGCGGGGAGCAAGGAGGGTGCGCGTTGAAGGCGACGGGCATATGCGC





GGTTTGAGGTCTGCGGGTCAGGGTCCGGGCACTGAAGTGCACGTGGTGG





CCGGCGCGCGTGTTTAATCATCCTGGTTCTCCCAGCTTGAAGAATGTTT





GAGCTGCCGGGCGCGTTGGCTCACACCTGTAATCCCACCCAGCACTTTG





TGAAGCCGAGGCGGGCGGATCATTTGGGGTCAGGAGTTCGTGGTGGCGG





GCGCCTGTAATCCCAGCTCCTCGGGAGGCTAAGGTGGGAGGATTGCTTG





AATCCAGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCGACACTGCACTC





CAGCTTGAGTGACAGAGCAAGACTCCATCTCAAAAAAAAGAATGTTTGA





GCTGAAAGAGCTCAAGTAATTATCCCTAGAGGAACTCCCTCGTTTTTGC





AGACAGAGAAACAGAGAAACAGAACCATAGTTCTAGAAGGAAGAAACAG





CCTTCTTGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene TOMM5. In some embodiments, the sequences from gene TOMM5 comprise or consist of:









(SEQ ID NO: 966)


AGGCTGGAGCGCAGTGGCGCGATCTCGGCTAACTGCAACATCCCCCTCC





TGGGTTCAAGCGATTTTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTGT





AAGCACGCGCCACCGAGCCGGGCTGATTTTTGTATGACAGATGTTATTA





AAGACAGCGTGGAGAAACCAGAGCCTGCGTTGCTGAGCTTGCGGCCGCC





CGCTTCACACCGCACTTCAGATCAGCTGGGTCAGGACGTGGATTGTCCC





GTGCAGGCATCCATCGTCGAGTGTGGCAACGAGAGGGCGGGAACAGGAA





GTGAGGATAGGCCGAGTTCCGGGCGCGAGGCGGCCACCGTGGAGAGCAG





AGCGCGGCGGCTGGAAGCTGCTAAGTCAGAGCCGCGATGTTCCGGATTG





AGGGCCTCGCGCCGAAGCTGGACCCGGAGGAGATGAAACGGAAGATGCG





CGAGGATGTGATCTCCTCCATACGGAACTTTCTCATCTACGTGGCCCTC





CTGCGAGTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CBWD5. In some embodiments, the sequences from gene CBWD5 comprise or consist of:











(SEQ ID NO: 967)



GTAACTAACCATCCCAGTCACAAAATGCCGTGAACTCTGGTGTCA







TGGGATTTTGCGGGCTGCCGGTGCCTCTTCTCCTGAGGCATTGGG







GCCTCATGATCAAGAGCAAATGTCTTTGAGGTAGGGGCTGGGTCA







CTTGCTTCTCGTATGGGTTACCTCTCATCTTGGGAGTTAATGATG







CCCGGTCCCCTCTGCGTTAGATTCCCATTTCTCAAGTGAGATGGA







AAGTTAGCCTTTGAAACAAGGCTTTGAAGCTAAGACACGCCCCCC







TAATTGTCTCTGTGACCTCTTACCAAGTTATCTTACCTTCCTGAC







GCTCATCTACCTCACCTGAAAATGGAAAAATACCTGTCACCTGTG







AAATAGGGCCGGAACAACCTACCTATTGCCATTAAAGGATGTCCC







GTCTGTAAAATGTCCGACACGTGGGGCTTTCGTCAGCAGATTCCC







CTTCCCCAGGAGCCAAAGTCCTCCAGCCAGTTGGCAGGAAGTGCT







CCTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CBWD5. In some embodiments, the sequences from gene CBWD5 comprise or consist of:











(SEQ ID NO: 968)



CTGAGGTCCAAATAAATAATGAGGAAAAAGTAAATAAAAGTGATG







CATGAATACTAAAAATTTGGAATTACATATATAATATGTAATAAT







AAAAAAAGAAAAAATTGTCACTGGTTATCCTTCAGGTATAAAAAT







TACAATTAAAATTGTAATGTTATAACCTTCCATTCTTTCCCCTAT







GAATTAAAACTTTTTTTTTTAACGAGACTAAAAAATCAAGTTGAT







AAAATCTATGCCCTGTGGGAACTGGTTAGGTCAGGTTACCCACTC







CAGATTTCCAGCAGGCTAGCCTCAGCTCTACCTACAGCCAGTAGC







TTATTAAGGCTCTAGGCGCCAGTGACACCTTCAGACAGGTGGTTA







GTGCGCCCTAAGGGCTCTCCGCAGCGTCGCTCAGGTTCACAGAAC







ACGCCCAGGGGCGTGTCCAGCTGTCGTCGGGGAGAGCCCACCTCC







CCGGGGGGTGTGGCTAAGGGACGAGGCAGTTCTCGTCCAGAGCCC







AGGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene TJP2. In some embodiments, the sequences from gene TJP2 comprise or consist of:











(SEQ ID NO: 969)



AGAAAAGCAAGACAGACTGAGAAAGTGTGGCTTAGCCGCGGTATG







GAGGATGGATGGTAGCACAGATAGGCTGCAGGGCCCCTAATTTGA







GTTCCTGCACAAAAACGACCATAATATCAACATTGTACCAAAGCC







AAGTGATCTCAGGGAGAAAAATAAAAGATGCCTTCCCTGGCGAGC







ACATCAAGGTTTCAGTCGCTTGGCTGTGTCCTTGGGAATTTTCTT







GAGTCCCCACTCCCAGGAGCCACATCAGACCCCACTGACCGTCCA







ACACAAATTCCTCTAGATGGAAGGGATGGATGATGACCCCGAAGA







CCGCATGTCCTACTTAACCGCCATGGGCGCGGACTATCTGAGTTG







CGACAGCCGCCTCATCAGTGACTTTGAAGACACGGACGGTGAAGG







AGGCGCCTACACTGACAATGAGCTGGATGAGCCAGCCGAGGAGCC







GCTGGTGTCGTCCATCACCCGCTCCTCGGAGCCGGTGCAGCACGA







GGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene TJP2. In some embodiments, the sequences from gene TJP2 comprise or consist of:











(SEQ ID NO: 970)



GCATAAGGAAACCCAGCCCAGAGCCACGAGCTCAGATGAGGAGGG







CTGCTAGCAGCGATCAACTTAGGGACAATAGCCCGCCCCCAGCAT







TCAAGCCAGAGCCGCCCAAGGTACGTGGCTGGGAAGCCCAGGATG







GGAAGGAAGAGGAAGCAGATGCCTCTGAAGCCTCCTGGACGGCCA







GGGAGGAGCATGCACACTGAGATGGTGTTTAATTACGGTTCTGAC







TCACTGTGTTCTCTATTAGAGCCTATCTTTTGGCCTGTTGGGTGA







ACTCCCAAATTAGGGAAGGGCAAAAAGGAAGGAAAGGCAATATTA







GGAGAGCATATGATTTTAGGACTGTTAGTGTTTATTTAATTTACT







CTTCTTGTTAAAAAAAGATTGTAAAGAAAAAGATTGAAAAATACT







GTACTGTATACCCAGAATCACCAATCGCAGAACGTGTTTTTCTTT







TGCTGTCCAGCTAAAGTTTTCTGCCCAGTCTGATTTAGATGCACC







AGAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ALDOB. In some embodiments, the sequences from gene ALDOB comprise or consist of:











(SEQ ID NO: 971)



GTAAGGATATTGCCCACTGAGATGAATCATGCCAGTGTAAGCTAG







AAATGAAGGCAAGTAAAGAAGGAAGCAAGGAAAAACAAAACACAA







CTATTACACTGGTCTTAGAGCCACACGTACCTGGTTTTCAATACC







AGCTCCTCCATTTAACAACTGTGACCTTGGGCCAGTCACTGAAAT







ATTGTGAGCCTCAGTTCTCCTATCTCAAAATGGGGATTTCTCAAA







ATGTTGTGGGGATTAACTTAGACAATGTACATAAGGTGCTTACCA







CAGTACCTTGGACAAAGAAGATTAAACTCTGCACCCGGAAGTATT







ATTTTTAGAAAAAAATTAGCAGCAGTAGAGTGGAAAGTCCCCACA







CTTTGAGGTCAGACACACCTGGGTTTGAATCCTGGTTCTATCACA







TGCTACCTAGATAATTTTGCAAGGTACTTCTGCCCAAGTAGACAA







GCATTAACTCACAACATTGGGTTAGCTAAGCAGAATTTGAATCCC







AGCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ALDOB. In some embodiments, the sequences from gene ALDOB comprise or consist of:











(SEQ ID NO: 972)



ACTTCTCAAAAGTGAAAACTAGTATCCATTGAATAAGAGTTTTTA







CCATTCCAAGCATTCATTTAATTCTCATAACCTCTGGTAGTAGAC







TCAATTATTACCCTTATTTAGAGATGAAGTGAGAAAACTGAACTA







CGAAGAGTAACATAACTTGCCCACACAACAAGGCAAGGTCAGAGC







CAGTTTAGGCTAATTCTCAAGCTCATGACTTTTGCCTTTGCTATG







TAGAGAGTAGAGGTTGTATCCTTAATTCTCTTTACCTCCTGTACT







AACACAACACCTGGTGCATGCAGGTGCTCAATAAATGTTAGGGTA







GACACTCTCACAGTGTACCTTCTAGGTAACTTCCCTTCTGCTATG







GACTGAACTGGGTCCCTCGCACTAATACACTTTACTAATTCAACT







CCTTGATCTGTAAAAGATGCAAACTGTTAGTTAGTTCAAGAGTTC







TGCTTGTGTTTTCCTGACATCTGACACCTGTTAATTATTTGTATC







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PTGR1. In some embodiments, the sequences from gene PTGR1 comprise or consist of:











(SEQ ID NO: 973)



GTAAGTAGACCTCCTGCTTTCTTTCCTTCCCTTTTCTCCCTCCTC







TAACCCCCTTGCTTTCTTCCTGCCAAGTTTCTAGACGCCCCAGTG







GAGGAGGTTTGCTCTTAGACCTCCTCCCCTTACGTCGAAATGACC







GCTGGGTCTTGCACTCGGAGGATCTTGGGAGCCGACGTCCCGACG







CCTCCCGCCCCCGCAGTTCCTTGGAGAGCTTGGAGCCGCGCGCCG







GAGGGAATAGGAAAGCTTGGTTACAACCCGGGACACCCGGTAGCT







TCCTGCACCCGGGAGGGGGCTGGCCAGGGAACTTGCTGGACTGCA







CAACTGGGGGAGGGTAGAAGAAGAAAGATCGGGGTGGGGATTCGG







GAATGGGCAAGCGGGCGGCGGGCGGCGGGAGGCGAGGGTGGGAGC







AAAGGGCGAGCGGGCAAAGGCCGGGCGCCGCGCCAGGCCCCCTCC







TCGGGCGGGCGAGACTTTCCCGGGACCTGGCCCCTCTGCGGCCAG







ACCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PTGR1. In some embodiments, the sequences from gene PTGR1 comprise or consist of:











(SEQ ID NO: 974)



GAAGCCAGGTCAAGGCCTGATGTTTGCTAGGGTGTGATGGGTGGG







GTGCCTCAGTGGCAAGAGTGCTCACTCCTGCCTGGGGGAGTCTGG







CAAGGGTTCACAGAGGTGACCTTGAATTGGCTGGTGGAAGGCGAG







TAGGTGTTCAGCAGGCGGACTGGGGACAGACAGGCAGGGTGTCTG







CACCGCAGCCCTGGGCGAAAGCTTGCACTGCGGGCAAGCGGCAGG







GCTGACGGGGAGCCCAGGGATCGGAGCCAGCACCGGGGGCTTATT







AGGGCTGCGGCCGCCCCAGGCCCTGCCTTTGGACCCTTCCGGCCT







GCTTGGCAGATGCCACGGTTGGTTCCCCCACGGGGCATATTTCCG







CCGGGCTCCAACTTAGGACCCCAGGCGGAGGTCCAGTGTCAGCTG







GACAAAGCTCTGTTAGGAATTTTCCTATCAGGAGTCCCTAACCCC







CTACACGCACACACACGGGCGCGCGGGCGCGCACGCACACACACG







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ORM1. In some embodiments, the sequences from gene ORM1 comprise or consist of:











(SEQ ID NO: 975)



AGCCTCCTACCTCTGCTGGAAGCCCAGATCCCATTGTGTGCCAAC







CTAGTACCGGTGCCCATCACCAACGCCACCCTGGACCGGGTGAGT







GCCTGGGCTAGCCCTGTCCTGAGCACATGGGCAGCTGCCTCCCTT







CTCTGGGCTTCCCTTTCCCTGCTGGCTGTGGTCGGACCCCCACTC







CCGGCTCTGCCTTTTTCTCTTCTGGGTCCCCAGGGTGAAATTCTC







ACCAGCCCAGGGGACTCTGGAGGCACCCCCTGCCTCCAAACACAG







AAGCCTCACTGCAGAGTCCTTCACGGAGGACGGTTCTGTGCTGGG







CCTGGAGGGGCTGCCTGGGGGGCAATGACTGATCCTCAGGGTGAG







CTCCTGCATGCGCACTGCCCACCAGGGGCCTCATCTCCCCATCTG







CAAAATCAGGGAGAGATCTGCCTGAGTCTCCTCCCAGCTGACAGT







CAAAGATTCAGCATCAAGCCCCCATCACCAGCTCCCACCTTCTCC







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ORM1. In some embodiments, the sequences from gene ORM1 comprise or consist of:











(SEQ ID NO: 976)



TGAGAGCCCCCATTCCAATGCACCCCCATCTCAGCTTCTGGCCAG







AAGACCTGAGCAAGTCCCTCCTTCTTCCTGGCCTTGGCCTTCCCA







TGGGTGGAACCGGGAGGGCTGGCTTTAATCTCCACCAGACTCTTG







CCCCAGGACTGTGATGGGCGATTGGCCACTTCTCAATAATCTTCC







TGTTTTCCTTCCGCCTTCTGTTTGGCTTTAGACAGGACCAGTGCA







TCTATAACACCACCTACCTGAATGTCCAGCGGGAAAATGGGACCA







TCTCCAGATACGGTGAGGGCCAGCCCTCAGGCAGGAGGGTTCACT







GTGGGAACAGGGCAGGCCAGCATAAGGTGGGGGCTGGATGTAGAG







CCCTGGAGGCTTTGGGCACAGAGAAATAACCACTAACATTTTTGA







GCTCTAACGATGTGCTCAGAAACAACTCTAAGAGGACACTGAGAG







AATTAGAGGAGGAAAAAGAAGAACAGAGACCTCAAATAGTTTCCC







CAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ORM2. In some embodiments, the sequences from gene ORM2 comprise or consist of:











(SEQ ID NO: 977)



TAGTTTCCCCAAGGTCACACAGCTTATAATTAGAACTAGAATTGG







AACTCCAGGCTGGCTTCAGATCTGCCTCTCTCTCACGCCCTCTTT







AAGATCCTTTGCAAACCAATGGTAGAAGCCTGTATGTTGGAGAGG







TGGTACCTTCAACTATGTCCCCCATCACCGCAGAGGTGGCACATG







GCAGGGATCTGATGGAGCTGAACTGACATCATTTAGCATCCCGAG







CCTCCTCTCTGGGCCTCATTTTCCTCCTCTGTAAAACGGGGAGAA







AGGCCCTGACAGCCACAGTCTGTGTGAGGCTCCTGAGATCTCATG







TACAGAAAGTGCTTGGCGTGGAGCTGGGCACGCAGCAGGGGCTGG







GCACACGGTGGCCCAAAGGAGACCCGGGCCTTCACTGATGGGCTT







TGTGGCCCCGGACACACCTAGGACTCCTCACCTGTAAGACAGGCA







CCATTGTGCCATCCCATGTTCTCACCCAGAGGCTCTTTTTCTCTT







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ORM2. In some embodiments, the sequences from gene ORM2 comprise or consist of:











(SEQ ID NO: 978)



TAGGCATGCTTAGCAGCCCCAAACTCATGCCCCTCTCAGGCCTCA







CCCCCCATTCACCCACCCCTGGGCTGGCCCCTAGAACCCCAGCCC







TCCCTGGCCTCCCGCCGGGCCCCACCATGTCCCCAGTCAGTCTCC







TTGCTCCCCCTGCAGCTGACAAGCCAGAGACGACCAAGGAGCAAC







TGGGAGAGTTCTACGAAGCTCTCGACTGCTTGTGCATTCCCAGGT







CAGATGTCATGTACACCGACTGGAAAAAGGTAAACGCAAGGGATT







GGACAGTGCCCACCTTGTCCATGGCCCAACTTGGGCAGCCCCAGA







GGCCCAGAGCAGGAAAGCTGCCAGGCAAGGCTGCACAGCTAGGCA







GATCTTCTGCTTTTAGGCACCTGCCTCACTGTAGGGACAGCTGAG







CTCTACAGAGGCCCAGGGGTGGTGGATGAGAGCCCAGGAGGGAGA







AGTCCCTGTGAAACCAGGGAGGACCTGAAAGCTAACAGGAGGGAA







CAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene C5. In some embodiments, the sequences from gene C5 comprise or consist of:











(SEQ ID NO: 979)



GTAAGTAAAACAAGCTATCTTCATTTTTCAATGCATCTTAGTTAT







GACGAATGAAGAGTTAAATTTAACAGATTCTGTTAACTTGAAAAT







AACTTGCTACTTCTGAGAACAGCAAGTGAATAGTATCATCTCTGA







ATGAGAAAGTCATGGAAGCATTTTGATTTGATGAGTAGCTAACAA







CATGACAGCTTTTATGGAATTGTTATTTATTAAAATTTACGTTAA







GTGATTATTCAAATTCCTCTTCAAAAAAGCACAAAAGAAACTCCA







GATAATAAACTTTCTTGACATTTTTTGCTTATTTTAACTTGGTGT







TTAGTTTTCTTTGTGTTTTTCATAGACTATACACAGTTAAACCAG







GGTTATTTAGGAGTCAATCTAATACTGTGATCTGAAAATCTGTTG







TTGTAGTGACTTCTACCATACATTTATGAGCTGACTTTTCACAAT







TTTTAGTGTCATTATTGAGATAATATGCATTTTAAGTAATGTTTT







AAAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene C5. In some embodiments, the sequences from gene C5 comprise or consist of:











(SEQ ID NO: 980)



CTAGGTTATGCTCAGACCATTTGCAGCACCAAGGACACTGCCATT







CAGGTCACCAAAAAAAGATCATCTTGTGAGCCCTGTCTAAACCAG







GCCCCTTCTAAAATCATTTCAGTGTGAAACTGAGTCCAGGTGGGT







CTTCATCAGTATTAGAGTGGACCTGCCAGATGTATGCTTGCAGAT







GATGCAACATCAAAGCAAATGCCCTGGGCAAGAGAAGAAAGGCCA







AAGCAATGGGCTTACTGGTTTCTAGCTTTGAGCCACTCTTCATAG







TGCCAAATTCCCATTTTAGGGTCCCTTTATTTGTATTAATAAAAA







TAAATTCCCTGGGTGTAAGTTCCTGTAAAGTACTCCCTATCTGTA







TTCCTGGGCATGAAAGGGTGTTTTAGTTCCAATTCTAGTTTTCCC







ACTTAGAGGTTCACCTTTCTGACATTAATAATTAACCAGATTATC







TGAATTCTTCAAGTTCAGTTTATTTAAAAGGAGACTATCCTCAAA







AGTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene DMD. In some embodiments, the sequences from gene DMD comprise or consist of:











(SEQ ID NO: 981)



CGTCTTCACCTCTTTTCAACCTGCTCCTCTTTTTTGCCTTAACTC







TGATGGCAGTCACACAACTGTGTGTTTTTCTTTTAATTCACTTTA







CACCTTGGTTTGGCTATTGCTTTCCATGGTTCATACTTAATTGGC







TGGATTTTATTTATTTTGACCTTTCAGTAAATTTTTTTGCGGCTG







AGTTTGCGTGTGTCTCCTTCACCACCTCATTTTTTGTTTTGCAGT







CCCGTTACTCTGATCAACTTCTGGCCAGTAGATTCTGCGTGAGTA







CTTTTTTTGCTGAAGGGTGCTGCTACCACCAACACATTCGCTCGC







TTGGTTCTCTTTTGTTCTGTTTATTTGTTTGTTTTCTTTTGTTTT







GTTTTGTTTTATGGACATATTAATTCCCCTTCCTTCATTTCAGTT







TTTTTTTTTCTTTTCTTGATGAGACTTTGAAAGTACCATCTACAC







AGATCAGGAGAATCAGAGTGGGTTTTTTGGTTCCTCCACCAGGTG







AAAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene DMD. In some embodiments, the sequences from gene DMD comprise or consist of:











(SEQ ID NO: 982)



AAAATACCATAAAGTGTGGTAAAATTTAATGTCACCTTTTTGACC







TTAAGACCTATAAACTACCATTTATTGAGTAGCCTAGTAAGCTTG







TATGTTTCTTTTATTTATCATAACAACGTGTTGAGGTGTTGTTGC







TCCCATTTTACTGATGATCAGAGCCTCTGAGAAGTAAATAACTCA







GCCAAATTAGTACATGCCAGGCCTGGTTTCAGAGCCCCATTTGTC







TGATTCCAAAGCCTTTGCTCACAACCACAACACTCTCCTTAATTA







CAAAACAAGTGTCATGGGGCAGAAGACTGGAGTGGTCATTAGTTT







TGAAATCATCCTGTCCTAAATCTGATCTCACCATGATCTCCCTTT







TAGACTACATCAGGAGAAGATGTTCGAGACTTTGCCAAGGTACTA







AAAAACAAATTTCGAACCAAAAGGTATTTTGCGAAGCATCCCCGA







ATGGGCTACCTGCCAGTGCAGACTGTCTTAGAGGGGGACAACATG







GAAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene GJB1. In some embodiments, the sequences from gene GJB1 comprise or consist of:











(SEQ ID NO: 983)



TTGCCCAGGCTGGTCTCAAACTCCTGGCTTCAAGTGATCCTCCCG







CCTTGGCCAAAGTGCTGGGATTACAGGCATGAGCCACCATGCCCG







GCCCTGTCATACAGTTTTAGAGGCTGGGAGCTAGAAGGGTTGACC







AGGATGGCAGCCTGTACAGGATCCCTCTCTGCAGCTTGCCCGCAC







TGTGGATCTGCCTAAGGCCAGGGCATGGGGGAAGCCTGAGGGATG







CTGAGGAGCACAGGTCCAGCTCAGTCTCCCAGAGAGACTAAACAT







GGGTGGGTGACAATGATGCCTCTCTTCCTCTGGGACAAGGCAGTG







ACTCCAGGCTATGTGATCTGTCTCTCCAAACCCATAGGGAGCTCA







GAGGATCAATCTTGTTTAATGATTGTATGTGGGAGGAGGTGGGGC







TGGTTAATGTTTGTCTGGTTCCTCTTGGGCTGTGGGCCATTTCTG







GCTCTGGCCCTGGGTTCGTTGTCCCTCCCCTGGGCTGGGCTGACA







GTAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene GJB1. In some embodiments, the sequences from gene GJB1 comprise or consist of:











(SEQ ID NO: 984)



TGAGGAGACTGGGGGCTGAGGGAGGTGGGCCAGGGAGGGCTGATG







TGGCACAGTGGGCCGGAAATAGGGGCCGGGTCTTGCCTTCTACTG







ACCTCCGCCTTCAGCCACTTAGGTCCCTTCCATGCTTCCCTCTGA







AGCTGGGGTGAGGCCCCTCCTGCCAGGTGCCAGACTTGCTTAGCC







TCTCTCTGCAGCCTCTTTCCAGGTTCAACATTGCTTTTCCTCTCA







CTTTTTTTCCTCTGGCCAAAGATTCCCCTTATTCCCATAGGGCCA







GATTCTCAGGAAGACTTGAGGGACCCCCTAGGTGCAAGGTGGCCA







AGTTTGTCTCTCTGAGACATTTCCTGCCACACAGGGTTAGGGTTT







AGTGGGAATGTTGGGCACTGCCAGGTGCTGTTTTCCTTCCTTGAT







TACAAGGCAAGCCAGGCATGCACCCAACACGGGGCTGCGAGTGGA







TGAATAAAGCGTGCTCAGCGGGCTGCAGAGGGCTAGCCAACAGGG







CCAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene TXLNGY. In some embodiments, the sequences from gene TXLNGY comprise or consist of:











(SEQ ID NO: 985)



AGAAGAAATTGTCCTGGATCATACAATAAGCTAGGATTTGCTCCA







GACCATTTTTTTCATTTTATCAGGCTACCTACAATTAAGGAATTT







CAGAAGATTTTGTGAACAAAAAATGTCCAGGAATTATGTGTGTAA







TAAATGAATTTGATATTTTGATCTCCTTATCCATCTTTTTCTTGA







TAAAATATCAGCTTTTTTTTTTCCAATGTAGGGCAGAAGCAAGAA







GTACAGATATTTTGATGGGGACAGCCCACGTCTGATAGGGTGGGA







CATGGGGCAGCAGTTAGAGGGTTGTGCTCTTTCTAGTGTGGGATA







GTTTGCAAGATGATATGTTGTAGCCACTTCTGCAGTGACGGGGAC







AGAAGGAGTCAGTGTTCTTAACTACAGTGGTAGCAGCAGAAAAGA







AGGGTAAGCAGGGTTCTAGAAATTTGTGTTATGTTTTCTCCCCAC







TGTATTTATTTCTTTGGTTAGTGGTGCAAGAAATTCTGTTTTCCT







GTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene TXLNGY. In some embodiments, the sequences from gene TXLNGY comprise or consist of:











(SEQ ID NO: 986)



TAAGTTCTACTTCTTGGAAAAAAAAAAAAGATCGCCAAAATTGTA







ACCAAAGCCATTTAAAACAAACTCCTCACTTAACATAGTGCCAGG







GCATGCAATTTTGTTTCTTACTCTCTGGATGTGGGATATGCGAGT







GTGTGTGTGTGTGTGTGTGTGTGTGTATTAAGCTTTCTGTTTCTG







ATGAAATTTTCATATGAAATTTACTGGAGATAAGATTAAATTAGT







GGAAAAACAAGATAATTTTGCTTTCTATGTAGTGGTCACCAAGTT







AAAGAATTGTGCCAGTTATTAGGCAAGATAGGCTTTGAGATTTAG







GGACAGTTAAATTTTACACCAACTTAGTGAACATGAGACTTCTAC







CTAGTGTTACATTTCATTTTTAAATAAGCAATTTAAAAATTGGTA







AATGATTTGTTTACTTTGATTATATTCTGGTATAATTTTCTGACA







AAATTATCTGTGTCTTGGTCAGCATTGTTGCTAGAATATGTATTC







AGAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MICU1. In some embodiments, the sequences from gene MICU1 comprise or consist of:











(SEQ ID NO: 987)



GTACTTTTTAAATTGATCTTTGAAGGCTTGGGCTCTTTAATGGTA







GTTTATAAATTATTAAAATTTATTCATTAAAATATTACTCTCACA







TTTCTGGTGGCTGGCAGTCTTAAAAATATAAATTAAACTGAGTTG







TAGTAATTAAATATAAATTAAGCTGAATTATAAACATCTTTTGGT







TTAAAATAACTAATATAACTAACGAAGACCACAGGGTGCTCTTTA







TTTTTACAGTACATTTTTTCTATACTTCCTTTGTTTGAAAAATGG







GGTGTAATTTTTGGTAGTCCCTCTTACCAAACCCTCAAATTAGGT







ATCTTATTATTAGTACAATGGATTATCTTCCTAAATACCTTTGTC







TTTAACTGCATTCTGAACCAAACCAACAAATACATTAAAAATTAT







GTCAGAATAACGTATAGACGATTCCATTATCAATGTTTCTAATCC







TGGGAATTCTATTTTAGAACAGCCATGGCACAAACATCATACAGA







GTAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MICU1. In some embodiments, the sequences from gene MICU1 comprise or consist of:











(SEQ ID NO: 988)



AGGTTAAGAAATTTTGTTGTAATCACTCTTGTTAAGCATAAGAGA







TACTTAATTAATTGAGTCACTTAAATAGTCTTACTTGGAGGCCGG







GTCTGGTGGCTCATGCTTGTAATCCCAGCACTTTGAGAGGCAGAG







GCAGGAGGATCACTTGAGCAACCGGGAGTTCAAGACCAGCCTGAG







CAACATAGCAAGACCTCATCTCTATGAAAACATTTAAAAGCAGTA







GTAGTCCTAGCTACTCAGGAGACTGACGTGGGAAGATCGCTTGAT







CCCAGGAGTTGGAGGTTGTAGTGAGCTAAGATTGTGACACTACTC







CAGCCTGGGTGACAGAGTGAGACCCTGTCTCTAAAACAAAAACAA







AAAAGTCTTATTTGGGATATTATGTTCCTACTTGAACTGTAACAG







GACTGATGAGACAACAGTAACTTCAAGTTAAAAATTAATCTTTTT







TTCTTTTGAAATTTCTTACATAATAAATACAAATAAGCAGTTGCT







TGGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene VDAC2. In some embodiments, the sequences from gene VDAC2 comprise or consist of:











(SEQ ID NO: 989)



GGCGGCCAAGCCGGAGGCCCGCGCCGGACTCGGAGTCGGCCCTGG







CTTCCTAAGATGGCCGCGCTGCCCCGCGGCCCCTCGCCCCTCTCT







GCCCGGGATCTCCCTTTGGCCCTTTGACCCCAGCTTACCGCACTT







CTTGTCCCTCCCGCAGATTCCCCTCTTCCCGCGGCCTCGCCATGG







CGACCCACGGACAGACTTGCGCGCGTCGTAAGTAAAGCTGGGATC







TCTGCGGGATGGGGCGGCGGAGAGTCGAAGCCTGTCGACCAGAAA







CCCAGTTTGTTTCCCCCTATCTTTCCAAGAACTTGGAAATTCGGC







TGCTTTTGGTGGTCGCCCCACCGTCTGACCACCTCCTCTGCGGAG







GAGGGGCTGGGCTGCTGGATTTCAAATGGGGAACAAGGCCCGGGG







AGTTGGTCTGTGGCCGCATGGACTTTTCAGCCTTTGTACATGTCT







TTGACGTATAGAAGTAAAAAGTTGTTAATTGGGGATTCTGCCTTT







GGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene VDAC2. In some embodiments, the sequences from gene VDAC2 comprise or consist of:











(SEQ ID NO: 990)



TAAGTTTGAAGCGCTATTTGATATTTAGTTGATGTAGACATTTGA







GTGAAACACACATGGCAGTATTTTTTCCAAGTTTCAATATTTAAA







TTCCCAGTATTAAATTCTCTTCCCACTCCAGGGTGTTGGTTTTCC







CCTCAGCGAAGATATTAAAGGACGTATCAGGGTAAAGGGCCTAGT







CTTAGGTTTCGGTTTTATCTAATTTAGTTAAATCAGCTCATCTCA







GTGAATTTTGGCAGATACATAAATATCCATACACACAAATACTTG







TTTGATTCTTTGTACTTGTCCAGTCCTAGGAAGTGAGGCTGTAGG







TAATAGACTGTTTTTCCGTTTTGAAACGAATTCCCCAAGTTTGTA







TAATCATGGTTATCTGTTTTAGAAATAATTCGTTGGTGGTATCTC







CTTTATGGGTCGTTATAGTTCTATTGTAGCTCCTGACCGCATAAA







AAACATTCCAACAAATGGGATTGCTTGAATTTTAATATCAGCAGT







GGGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CYP2C8. In some embodiments, the sequences from gene CYP2C8 comprise or consist of:











(SEQ ID NO: 991)



GTAAGTCTGCCTTATGTTCCTCCAGCCAATTGCAAAGGGTAAGTT







ATTTGACTGCTATTTTTAGACAAAATATATTCCTGGAAGCACACT







ATTATAATAGATCATTGTAAAGCAAAATACTCCCTCTGAACTTCT







TTGATGTTTCTTTTGTCTTCCTATTTTTTTTTTTTTTGAGACGGA







GTCTCGCTCTATTGCCCAGGCTGGAGTGCAGTGGCACTATCTCCA







CTCACTGCAAGCTCTGCCTCCCAGGTTCACACCATTCTCCTGCCT







CAGCCTCCCCCGAGTAACTGGGACTACAGGTGCCCTCCACCATGC







CCGGCTAATTTTTTGTATGTTTTAGTAGAGACAGGGTTTCACCGT







GTTAGCCAGAATGGTCTCAGTCTCCTGACCTCATGATCTGCCTGC







CTTGGCCTCCCAAAGTGCTGGGATTACATGTGTGAGCCACCGTAC







CTGGCCTCTTTTGTCTTTCTAAGTCTGTCATTGTCAGAAATAGCG







GAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CYP2C8. In some embodiments, the sequences from gene CYP2C8 comprise or consist of:











(SEQ ID NO: 992)



TATACATTTATTAGCTAATAATTTGATATATGTATGGTAATTCAA







CATGTATGAGTTATATTCACTATTTCATGTTTAGGCAGCTGTATT







TTAAGTGAACTATACTAAATATTTGAAAGGCTTTTGTTATCAAGG







GCTAAGTCTCCTATTTTTTGATATAGCATTACAATGTACATTTTT







TATACACAAAATATAGAATACACTGATTTCCCTCAAGGTCATAAA







TTCCCAACTGGTCATTAATCTGAGAATATTGAATTTTGAGTATAT







TCTAACATAGAATCATTTACTTCAGTGTTTCTCCATCATCACAGC







ACATTGGAACAACCAGGGACTTTTAATTAAAAATACCTGGGCTCC







AATCCAATACAATTAAACCAGAATCTCCTAGATTGGCACTGGAAA







GAAGGAGTAGGACAAAAGAACATTTTATTTCTATCCATGGGCCAA







AGTCCACTCAGAAAAAAAGTATAAATTGGATCTAGGTGATTGTTT







ACTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene GPAM. In some embodiments, the sequences from gene GPAM comprise or consist of:











(SEQ ID NO: 993)



GTGAGTGAAGACCCTTCAGTGTTGCCTGGTCTCTTTTCTTGAGCG







TTTTCATGAAAACGTAACCCCCCGTGTTTTTCTGTTAGATAATGT







TTGACCCATTGTTGATACTTTATATTCCACTCCTGGATTTCAGTG







ACCACCAAACCAATATGTCATTTACTATCTTGGTTTGCTCTCTGG







GTCACTGCTCCACTGAATCTATCCTGCAGCTATGAGCCATGATAG







GACAGATTGCTCAATGTTCTCAGGCAGTCACATCCAAAGCTAGTG







AGCACGTATTGTGTGTGAGGCACTGGGTCAGTGGTTTGTAAATAA







TTAACACACCCACCTGTAGATGATGGAACCACAGATGGTAAGAGT







CAGAAGCAGCCTTAAAAATCATCTGCTCGGCCAGGTGCGGTGGCT







CACGCCTGTAATCTCAGCACTTTGGGAGGCTGAGGCGGGCAGATC







ACGAGGTCAGGAGATCGAGACCATCCTGGCTAACGTGGAGAAACC







CCGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene GPAM. In some embodiments, the sequences from gene GPAM comprise or consist of:











(SEQ ID NO: 994)



GCCTAAAACACATAGTGGTATCTGCTTTAATTTGGGTGATAGCCC







CTTGCTTGAGGGAATGTGGCTATAATTTTAGTTTTCAAACGAAAA







TGTAATCTCCTGTTATATCACTGTTTACAATGAGTAGTTACTGAA







TTACCGTTTGTATAGCATTACCCTTATTTTTCAGGAGAGGTAGAG







TGTCTTGCTATCCAGGAGCTGAAAATCTAACTGAGATGAGACATA







CACAGAGCAGCAAAGTGAGTGGAGGGCCTCATTTGTGATTAAGAG







AAAAGAGAGGAAGGAGAGAAGAGGGGGCTTATGTCTTTGGAGAAG







GTTTTTGATGCAGTTGGGATCATATGGAATCATAAGAATTTAGTC







TTATGGGATTTACTTAAATTTCTCCAAATACAGGCCATTATTTTT







GATGTATTAATAATCTCTTAAGGTTATTCAAGCCAGTTGAAGTTT







TTCTTGGAGACTTTGAGCTGGCAACTTCATTGTTGCTTTTCTTTT







CTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ACSL5. In some embodiments, the sequences from gene ACSL5 comprise or consist of:











(SEQ ID NO: 995)



GCTACCTATTAGCTACATAAAGTATCTTTCTCTCCACCTCTTATT







TCCTACAGGTGACCCCAAAGGAGCCATGATAACCCATCAAAATAT







TGTTTCAAATGCTGCTGCCTTTCTCAAATGTGTGGAGGTCAGTGG







TCAGTTGAAAAAGAGGCTCTCATTAAAATGTGAATCTGTCATAAG







ATTTTTATTTTTGAGGTTAGAGCAGGCAGACACACACACACACAT







ACACACACACACACACACACGTTAAAGGCTGGAGTGTGGACAAAC







ACCAGCGTGTTCTTTGTTTAGAGGACTGTACATTTTCAAGCGTTG







GTCTTTTAGGATCACTGCAAATTTCTACACTATATGCAGTTCAAG







GCATCTCAGATCTACACAGTGTACCGCCTATACAGCTGTCTGTGG







TAGTCCTGGGAGGAGGTAGTTTTAAAATCTCCATTGGAATTGAAA







GTTGGCATTAATCTCATGTTGCCTCCTCTTACTTCCCTGGCCTAC







ATAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ACSL5. In some embodiments, the sequences from gene ACSL5 comprise or consist of:











(SEQ ID NO: 996)



TGAGTGTTCTGTGTTCCTAGCAGTATGTGGCAAGGGGGTCCTGAC







TTGCTATCACATGTTTATTCCAAAGGCAGCTACACAGTATTTACT







GTTCTTTCTCCGGTGTGAGAGGTGCAGGCAAGGAGTTGGCTCATG







GGAGCCCTAATCATGGCATGGCCCCTCATTTCCTGCAGAGTCTTC







CTCTGCTAAGTCTGCATGTGTCCAGGGAGGATAGTACTTGCTCCT







GCCTTCATGGGGGTGTGTGAGTCTAGTGACATCACAGCTAGGAGC







TTACTATCTATCTGCCCTTAATTCTGAGCTGACACAAGACATTGT







CTTCTTTTTAAATTTTATTTTGGTTAGAATTTTTGAAAAGATCTA







GGATTAGAGAAAAGGAAGTTCCCTACAGAGGATCAGGGAAGGAGC







ATTTGATCTACATAAGGTGTGAGATGTCCCCCGCGCCGGTTTTTG







CACACCTGAGTGTGTAAGGTGGTGCTGTCAGCATGGTGCATACCG







GAGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene HABP2. In some embodiments, the sequences from gene HABP2 comprise or consist of:











(SEQ ID NO: 997)



TGAGGTTTAAAACAAAACTTTTGAAGCCAACAAGCAACTGTGGGC







AACACTTCAAAGGGCCCAGAATCAATGTTTGTCAATAGCAGTTTT







GCATCCTTTAATTTATGTCTCATGAATATAAAGTGTGATGTTATT







AGGAGCCCATCCATGCCTTAATTCTGTTTGAGATTTGATGAACCA







GAAGTCTATTAACATTCTGTACAAATCAGCACTGGCAGAGGCCTG







TGGCACAGGCTTCACCTGATCTTTTATCCTGAGGCTGGGATGCTA







GTCACGCTGTCAGGCTTCCTGCTCGGAACACCCAGCAGCTGTCAT







GTGCACCGTGGAGCCAGGGGGCTATGTCAGCTACTTTCTTTGGAG







AGTTTCTCCCCAGAAGAGAAACCACTTCATGACTCAGCCCAGCCC







CAAAGTTGCCATTTGTTTAATCAAGATAGTTAGTTTGCAAAACAT







TAAACATTAAACAGTATTTGATGCCTAGTTAATAATTTGCATCCT







CTTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene HABP2. In some embodiments, the sequences from gene HABP2 comprise or consist of:











(SEQ ID NO: 998)



TAAGTGTTCTTTTCTAATATTTGTAGTTGAGAGACTCCGAAGTTT







ACTAGGAGGACCAAGCTAGGCTGGGAGTGATGAGAAACTTTAGAG







TGAAAGGCTGGAGTTGCCAAGTTGGAGATGTTCTAACTACAAGAA







CCAATGCAGGCCTGTGGCTTGGTTTCTTTTGAGCAGCCACTCCCA







TGGGAACAGGTGTCTGACTTGAGCCATCTTGGAGCGGAGCCTCCA







TGAATGCATTCCAGCAAAGGAGACTGGGGCAAGAAAATGTGCCCC







AGGGCATGGATTGTAATCTGTCATGGATCTCATAGACCCCTTTGA







GAAGCCATGGAAAGCTATAAACTCTCTCCCCAGGAAAATATACCT







ATCTATTGGTGCTGACACACACAGTTTTCCAGCGATTTGTGGCTG







ACACTAGGGTAAGAAGCAATGTGCTATGGCCTTAGAGGGGAAGCC







TGCTGACTGCAAAAGATCCTGGCAGTGGGTCTGCATAAATAGTTT







GGAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene IGF2. In some embodiments, the sequences from gene IGF2 comprise or consist of:











(SEQ ID NO: 999)



GTAGGATTTGAGCTGTGTTTCCCGCCCTGATCCTCTCTCCTCTGG







CGGCCGGAGCCTCCGTAGGCTCCAAGCCTGGCCCAGATTCGGCCC







GGCGCAGCCGGCCTTCCGCGCGTCCCGCACCTGGCGGGGGCTCCG







GGGCTCCGGCGCGGCACCGGGGGGCGCTCGGGATCTGGCTGAGGC







TCCAAGCGCCGCGTGGCCGGCTCCTCCTGCTGGGGCAGGTGGCGG







CTGCGCGCCCCGCCCGAGCCCAGGGGCCCCCTCAGCCGCAACAAC







CAGCAAGGACCCCCCGACTCAGCCCCAAGCCACCTGCATCTGCAC







TCAGACGGGGCGCACCCGCAGTGCAGCCTCCTGGTGGGGCGCTGG







GAGCCCGCCTGCCCCTGCCTGCCCGGAGACCCCAGCTCACGAGCA







CAGGCCGCCCGGGCACCCCAGAAACCCGGGATGGGGCCCCTGAAT







TCTCTAGAACGGGCATTCAGCATGGCCTTGGCGCTCTGCGGCTCC







CTGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene IGF2. In some embodiments, the sequences from gene IGF2 comprise or consist of:











(SEQ ID NO: 1000)



CCGCATCTGCTGCTGTCCCGCCGGTGCTGGCGCTCGTCTCCGGCT







GCCGCCGGGGAGGCCGGCGTGGGGCGCGGGACACGGCTGCGGACT







TGCGGCTGGCGGCTGCGCTCGCTCCTGCTGGGCGCCCCGAAATCC







GCGCCACTTTCGTTTGCTCATTGCAAAGATCTCATTTGTGGGGAA







AGCGGCTGGAGGGTCCCAAAGTGGGGCGGGCAGGGGGCTGGGGCG







AGGGACGCGGAGGAGAGGCGCTCCCGCCGGGCGGTAAAGTGCCTC







TAGCCCGCGGGCCTAGGACTCCGCCGGGAGGCGCGCGCGGAGCGC







GGGCGAAGTGATTGATGGCGGAGCGAGGGGGGCGAGGGGGGCCAG







GGGGGCGCGAGATTCCGCCGGCGGCCCCTTCCCCTTGGCTAGGCT







TAGGCGGCGGGGGGCTGGCGGGGTGCGGGATTTTGTGCGTGGTTT







TTGACTTGGTAAAAATCACAGTGCTTTCTTACATCGTTCAAACTC







TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PSMA1. In some embodiments, the sequences from gene PSMA1 comprise or consist of:











(SEQ ID NO: 1001)



GTGAGAATATCTTTGCTTTTTGATAAAACACAGAATGCATAGATT







TCACATCTCACTATGTGAGCCCCCACAAAACAAAACCTAGAGGAG







CATGTGAGAAAAACGCATCTCTAGGCTAGAAAACAAAAAGAGAAA







AAAAGAAAAATGTCTGTGTAAGAAAGCAAAGGAAAGAAGATGCTC







AAATCCAGCAAATCATACCAGCCTTGTCATAGGCACAAAAAGAAA







GGGATTAACCTAAGTTACAAGGTGAATTATCATAAAATCAGATGA







ACCTTTCTGAAAAATAGTCATGGGACAAAGGAGGGATATATAACA







CTAGAGGCTTAACCCTTTCCTGCAGAGAAAAAAAATGTAACTGAA







AGAAATGCCACTCAAGGCTTGTGACATTCAAAGTGAAGACAAGAT







AAGGTCTTATGTAGTACATGACATAGGTGTAGACACTCACTAGAC







TGAAAGGAGTACATGTTTCCAAAGCAGCTTTAATTCACGTCTCTG







CTGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PSMA1. In some embodiments, the sequences from gene PSMA1 comprise or consist of:











(SEQ ID NO: 1002)



TGACCTAATTGTATTCATTTGTCAAGAGGCCGGTTCCATATCGGA







GACAGGCTGTATTCATAGATGATTTTAAGGACAGTTTAAGGCATA







GAGTTTTACTCTAAAAACTGAGAAACAAAAAATCCTTCATTGTAG







AATTTGTTTTGTTGCCCTAGCTCTACTATTTATACTTTTCCACTT







TAGCTATGCACAGAACATAGCTAATTTTCATCTCAATTTTTCAAT







TCTTTTGCTATATAATTGTTAACATTTATTGACCACTAACCTCAT







TTTAAGGACTGTGCTAAGTATTTTATATACTTTCCTGGGGAGGCA







CTTATTCCTCACTTTTGATATTTCCCTCCACGTGGGGAACATCCA







TTAGAGTAACTCTCCTGGCCTGTATCTACTTACCCCCAGGAGAGG







AGAAGAGAGTTTGGTGAGTTTGAGATGAGGAACAGGATGTCTGAT







GAAGATCTAATATAACTCATGTTCCTTTTACCTTCTCCATTGTCT







TTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene DEPDC7. In some embodiments, the sequences from gene DEPDC7 comprise or consist of:











(SEQ ID NO: 1003)



TGCCTTTATGGTATTCACCATTCATTGTTAATTCCTAGTCTTGCT







TTTGCTTTTGGAAATAATTTTGAATAAGTGCACTTTTTAACTCAA







ACTGTAACTTGAGTTTTGTTCCCTTTAATATAGTCATATTTGGTC







AATTAAAAGCAGTTTTTTTCTTTTGGCTAAAGTGAATGGGAAGAC







GGAAATAGCTTTAGAAGCTACCCAGCTCCTTCTAAAGCTTTTAGA







TTTCCAAAATAGAGAAGAATTTAGAAGACTACTGTATTTCATGGC







TGTTGCAGCAAATCCTTCTGAGTTTAAATTACAGAAAGAAGTAAG







TCTTTTGTTTAACTGTTAGTTGTATTTAATATCCTTAATACTTAC







AGGTTAAGTATTAGATTATTCCATCTTCAGCATCAAGTATGCTGA







AATGTATAATAGCTATTAATATTTAATATTCCCTTTTATATTAAA







CTTGTCTCTTAAATATATACTATTGGATATATTTGTTTTATTTTT







ATAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene DEPDC7. In some embodiments, the sequences from gene DEPDC7 comprise or consist of:











(SEQ ID NO: 1004)



TAAAATTGTGAAAGTAGTTAAATTGAGCTTATGTAATAGATCACT







AGATTAAAATGCATTTGAATAATGTCCCATGTCCCTTCTTTTTCT







TAAGATTCCTGGAACTCTACATAAAATTGTAAGTGTTAAGCTTAT







GGCCATACAGAACGGAAGAGATCCAAATAGAGATGCAGGTAATCT







GAGAAATATTATTTTCATGATCTTCCAAAGACAAATTTCAGGTGT







TTTTTGAAAATGTTTTGCTATAAGTAGTTGGACTTTAATTTCAAC







CAAATTTAAAACATTCTCCTCAGAGTTATTCATACAAAGCAACTT







TACAAGTGTTAGAAAGTCAGCAGTGCTTACCATCTTCAGTGCATA







TTACAAAATGGGGAAGAAAAACATAAAGACAAGAATATTGCTTGA







TTTTTCTGCTTAAATATGAGGAATTGAGTCATTAACTGAACTTTT







TACTTTTAAACTTTAAGGATATATTTATTGCCAGAGAATTGATCA







ACGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CTNND1. In some embodiments, the sequences from gene CTNND1 comprise or consist of:











(SEQ ID NO: 1005)



TGAGAGAGGAGACCACAATAGAACACTGGATCGATCGGGGGATCT







AGGCGACATGGAGCCATTGAAGGGAACAACACCCTTGATGGTAAA







TTCTCTTTATATACTGCTATCTGTCTAAGGCTAGTTCTATTTTGA







AGCCTATGTGTTTTGTGAAACACAAAAAAAAGTACAGAGAAAGAT







CGCATCCTTTTCTGGTAGGGGTTTTCAGGAAAAAGTAAGAGTTCT







GACTCATGTTGGGATTTCTTGGGCCGTTATTCTGCAGTGGTCAAA







ATGGGGGAAGCATGTCTGTAAAAGTGTTACTGATATGACTAACAC







TAACTGATCTACTTTCAAACATTACCTTTTTCCTCTCCCTCCCTG







TTTATGAATGTTTTGCCCTTCTCTTACTATTATATCTTGTTTTGT







TTGAGTTTACAAATTTTCAGAGTTTCCCTAGCTCTGGCACACACT







CATGAGGTTCCTGTCTCTACTCATACTAACAAATTGCATGTGTTC







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CTNND1. In some embodiments, the sequences from gene CTNND1 comprise or consist of:











(SEQ ID NO: 1006)



TATGTCCTTCAGTCACCCCCAAGATTGTTCTTGAGGAAGGAGGCT







CCTGAGTGCCAGCAACTTGTCAATCACGCTAATCTGATCAGGCCA







GAGACTAAGTAGTCTCAGCCACATGGGGCTCGAGGGGTTCTGCTT







TTGTGATTATTACCTCTTACGAGTCTGGGACATGCAGCTGGTGAT







CTGATTCTCCGTTATGTGCTGCTTTCCTTTCTCCAGATTTGCTCA







ACTTTTTTGGGGGGGGTCTCCTTAATCCCAACCCCATTAACATTC







TCTCTTTCTCTTTTTTCTCCACAGCAGAAGATTTAGCACCACTAT







CTCCGTTCCATCTGGGCTTATATGTACTTTTATTTTTTGGTGGTG







AAATTGACTGATGATTTTCCTTTTTCTTCGCTGGACTATTGTGCC







AACTGCCAGGCTGCCTCCTGCCCTTACAGCCCTAAGTGGCTGCCT







TCTTTCCATCAACTCCCAACTTCTTCCTGTGAAGTTTAATTGTCT







CAAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MS4A6A. In some embodiments, the sequences from gene MS4A6A comprise or consist of:











(SEQ ID NO: 1007)



GTGAGCAAGGTTGGAGGTGATTCTGCCCCTCTCCCCAGGCTTTCT







GTGTGAGTCCATTCCTCCTCTCAGATTTATTGTTAGAACTTAAGA







CAAGCCAATTACATTTCATAATGTCTGTGTCATTCAGACGCTGAG







AACTAATCCAGCCTTCTAAATGGTGCTTCTTAACTCCTTTCTTCA







ACAGGAGAGTTAATGTGTGGAATTAAGTGCAGAATCCATGGCCCT







CTGTGGTGACGGTGATGGTTCAGGTTGTGTTTCTGGGTTCATTCT







GGAAGCTCCCCCAAGGAAAGGAGGAAGGAAGCTTGCAGGGTGGGG







CTTTGCCATTGCCCTGACTGGCTCTGGTTTCCTTGCCTGATACAT







TGAAGTCAGCTTCCAAAGAATGCCACCCAAGGTTTTGAAGGGGCA







CAGTGCCTCTGTCCTCACAAAACCAGCCTGCATATGAGTTTACTG







AATTCATTTAGGTCCTGCTGAATCTTTCCCATTTGTTCCTTCTCT







GTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MS4A6A. In some embodiments, the sequences from gene MS4A6A comprise or consist of:











(SEQ ID NO: 1008)



TTCTGGTTGTTACACATAGGGTGGGATTGTGCTGGCATAGGCCAG







GGAGTCTGCTAAGCATCCTACAGTGCACAGGATAGCCACCTACTG







TAAAGAATTACCCAGTCCAAAGTGGCATTAGTAGCTTTTTTGAGA







AACCTGTCCCAGATCATTAATCAGAACGTGTTATTGATATACCAG







TCTTCTCCCTTGTAGTTAGGTTGGTAGAATTCAGACTCTGGAAAA







AACTGGGAATGTAAAAGGCTGAATGAGTGTAAGCTGAAAGACAAG







AGAAAGGAGAATTGTTTTAAGCTTACTAAAGCCAAAATGGAAAAA







AAAAGAGGGGGGAGGATTGGGGGGTGAGGAAGACAGTCAACAGAC







CGCTTTTAGGCCATCATTCCGAAGGGTTCTGTGCTAGGAACCTTA







CACGTGTTGTCTCACATAATCCTTAAAACTCAGAAATGATTTTAA







ATTAAATAGCACAGAGAGGAAAACTGACTGGAGAGGGAGCCTTTG







CTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SLCO2B1. In some embodiments, the sequences from gene SLCO2B1 comprise or consist of:











(SEQ ID NO: 1009)



AAAATGGGGCTAATGTACACCTCGCTCATAGGAGTGATGTGGGGT







TGCAGGGCAGAGTTACTGAGCCCGTAGTAAGCGCTCCATAAATGC







CAGCCACTGGTGTTGTCTGGCAGGAGCTGGCGTTACAGTGGTTGG







GGATGCTGGGGAAGTGTCTGACTGTCCCAGCTCCAGGTCTTGCCA







ACTGTAGGAGTAAGGGGAGAATTTGATGGGACCAGTGGGTGGAGG







AAGAGGAGTGATGTCTGTGACCAGAAACATACAGAGGTCAGCACA







GGACCCTGATGGTCAGTGGGTGTGTTGTCTTGTATATGGGACCAG







GCTCTGCAGGTTGAAAAAGGATGGGGTACGTCCCTGAGCCCCCAG







CCTAGATCATCAGATGGGAGAGAGGCTGGCTCTGGGTGGTGGGAG







AGAAGGGCATAATAAGAACCCTTCAACGTTGATGCATGGCCCTTG







GGGGCTGGAACCCCTCCAACCTCATGTTGCCCATGTCTCTGCTTG







TTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SLCO2B1. In some embodiments, the sequences from gene SLCO2B1 comprise or consist of:











(SEQ ID NO: 1010)



TAAAGATCTTGCTTGGGACCATTGGTGGTGGTGATGGGGTCCAGA







TGCCCACTGTGTGCCAGGCCTGGGGCACAGGCATGAGCGGAATTC







AATCCCTGATCTTAGGGCTCACAAGCTCATGGGGTGACAGACCTG







GCCCAGCTCTCCTAGAGCGGGGTATAGAGGCAGAGTCTAGACAGC







CCGTCAGGAAGCCACCTCGGATGCAGGGGTGGGGCGGGCTTGAGG







GCCAGACAGCCAAGAGGCCCCAGTAACCCAGAGCCCCTGAGGGGT







TGTACATGCCAGGGAGGGGGCAGGGTAGGACGGTGGGCCTTCATT







GTCCCCTGAGCACCACCTCCCTCAGCCTGGATGCCCAGCCCCGTG







ATCCACGGCAGCGCCATCGACACCACCTGTGTGCACTGGGCCCTG







AGCTGTGGGCGTCGAGCTGTCTGTCGCTACTACAATAATGACCTG







CTCCGAAACCGGTGAGACCTGGTTTGATGGCTTGTGGGAAGGGTG







CTGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PICALM. In some embodiments, the sequences from gene PICALM comprise or consist of:











(SEQ ID NO: 1011)



GTAAGTAAATAAGGAGAGCTTCAATAGTTCCATGGTAGAGAGAGG







CCTGCTGGAAGGTTCAGAAAATAGGCCGTTTGGAACCTTCTCCTT







TAAGCCAGGAGGGCGGCTTTGGGAGGCGTCTTGAAATACATTCTT







GACTCTGCTGGGAAAGCTCGGGGAGCCAGAGGCCTAATACCCCCC







ACTTCAGGGGAAGCTGTGAAGGCAGCTGCACCTCCTTTGTCAAGC







TGTTGGTTTCAACCACAGACACGAGGACACACTTGAGGTGGGGGG







ATAAGGGGAGAAGCCTGCCGCCCCGCCCCACCCCCGCCAGCACAC







CGCCCTCGCCATTCCTCTCCCGCCCCGCCCCGCCCGGCGTCTGTG







ACCCCGCGGGCCGAGCCTCAGGCGACCTGTTGGCAGGGAGAGTCA







CGTGATGCGCCGGCTGTGCACTCCCCTCGGGCGGGCCTCCTGTTG







CGCCCGCCTGGCGAGTTCCATCACGTAACGCGCGGGCGCCGCCCA







GCTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PICALM. In some embodiments, the sequences from gene PICALM comprise or consist of:











(SEQ ID NO: 1012)



TATTTTGGATTTGGCTGGCAGATGGTGTACACAGACTTTCTGAGA







ATGTTAGCATTTCATTTGAAACTAGCAAGATTACATGTGACTATC







ATGGTAACTTAGCACTCACATATTTTTCAACTTATCCCTAACCCT







GGGTTGAATGTAGAGGCTCTAATAACATATAAAAGGCTTTGTTAA







TGTATGGTCTGGGTCTCATTGAATAGTATTTCAGGATGTAATACT







TCTTTTAAAATGTCCACAGGGCAGTGTCAACGTCTTTCCATGGAA







ATCTGGCTCACCCAATAAGAGAAAAGAGGCCAAAGGTAAGAAAGA







CTAAACCAGGGGAGATGGCAGGCAGTGATGTCTGGACTGTTTGAA







TGGGACTTCTCCATTTACCGCCCCTCTCACTTCCTTCTATGGTCT







GGACCTTTAATCACCACAGCAGTCAGCCATGTAGGGTCAAGCTAA







TCGCTGTTGACATCATCCTACTACTTCCCTTTTCAAATTCCAGTG







AAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SLCO1B3. In some embodiments, the sequences from gene SLCO1B3 comprise or consist of:











(SEQ ID NO: 1013)



TTTAGTATGTGACTTGCAAATTTTTCTTGACAGCCAGACAAAATG







TATTGGGTAAAAGGAATTGCTGTATATAAGCCTTTATTAATGTGG







TGTAAGGCGATGAAGACAAAGTAGCATCCTGTAGTTCTATGATAA







GGCCTCAATCTTTTAGTGAGCCTGTACCTCTGGACTGTGAACTTC







AAACTTGCTTCTCAGTTTCTGTCTCCCACTCCTTAGGTAGAATGA







GATGGCTTGAGGGGGCTGGAGTTATGTATTTTCCTCCCTCCCAAA







GGAAGGCTAGAGACAGCTGGGGTTTAGTTTCTGTAGCTTCATATG







TTTTTTTAATAATAGAAAAATATCTTATGCCCCCAATGAAAATAA







TAAATAGAATTGTTCTCTTAATATTTGTATGTGTAACTTGTTTTT







CTTTCTTTTAAGATATGCATACTGGGGAGAAAAAAATGTAAGATA







TTTTACACATTTAAAATAATAATCGACTCTCTATTTTCTCTTTTC







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SLCO1B3. In some embodiments, the sequences from gene SLCO1B3 comprise or consist of:











(SEQ ID NO: 1014)



ACCCATTGATAAGATGTGTCTTCATTGCATCTGTCTGTTCTCTGG







GAGAAGCAAAATCTGTGTTACTTCCTTGCATACTTGTTTGTTTAT







TCATTGTGCACAAGAAGATATCTTCCACATCTTCACAAGCTGGAT







AAGAAATAGAGAAAATGAGATCAAATATTTAAATATTGCATTTTC







TGGTAGTGATGCAGGATATTTCTTGGCCTCTTCACTGGACTCACA







ATAGAGGTGCCCTGTTTATTTGGTCTGCCATGCTCAACCCCTTGT







GGGAGGGAGAGTGTGAGTGACGTAGTGCAGGACCATGTGGGCTGT







CCTGGTCATCGACACAGAAACAAGCTCTGTGAGTTTCTCATGGCC







AGACCAGGTGCGAGTGAGTGAATGCTGGACCTGGGCAACCGCTCC







AGGCACTGAAACGAGTGAGTTACATGCAGGGCTGGCAGCCAGGCA







AGGTGCAAGGGTGCTCAGGATCCTGAAGCCCCAGAGGGGTTGTTA







TGGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ANO6. In some embodiments, the sequences from gene ANO6 comprise or consist of:











(SEQ ID NO: 1015)



AATTACTGATGCCTGAGGCCACCTCCAGAGATCTGGTTTAATTAT







TGGAATGCAGCCTCCACTGGGATTTCTAAAAGCTCCCAGAAGATG







TTATTGTGCACCAGGGGTAGTGAACCACAGACCTGAGATGTCAGG







GTCCTGAGAGCTCATCTCATCCAGTGGTTCTCAGCCCTGGCTGCA







GATTAGCATCATCTGGAGAGCTCATGAAAGAAGCACTGAGGTCCC







ACCACCTGAAACGACCTTTCAGAGGAAGGAGCCATTCTTTTTAGA







AGGTGCTCAGGTGATTTTGATGCACAGCAAGAGTTAAGGTTAACT







GACATCTTAGCCATCCCATGTTATAGATAAGGAAAATGAGAGCAA







AAATGGTCAAATGGCTTGTACGAGGTCAGGTCCTTGTTGTTACAG







AGGCAGGTCTGATGCCGCCTCCTGCTCTGTCTGCAGCCTAACACC







ATCCCTTATATTGGCCTGTTGTATTTATTGATGCCTCCTCATCCT







TCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ANO6. In some embodiments, the sequences from gene ANO6 comprise or consist of:











(SEQ ID NO: 1016)



TAAGATCCCCTTCCTTTGTATTTGAACAGCATCCTTCCTTCTTTT







TCCCCTCCGACTGTCAGTTCCGGGAGAACAGGCCTGTCTTTCCTG







TTATTTCTGTAACAGTAGTGGGGTTTCAGATAATATTTGTTGAAT







TGAAGTTCTGTAACTTTTTATTCCTCAGTCCACATGGATTGCTGA







AAATAATCCATTCCTATATGCATGCTTCAGAAAAGAACTTCAGAA







AGCAAAAGTTATTTCTCACTGCAACCTCTGCTTCCCAAGTAGCTG







GAATTACAGGTGCATGCCACCACACCCAGCTAATTTTTGTATTTT







TAGTAGAGACAGGGTTTTGCTATGTTGACCTGGCTGGTCTTGAAC







TCCTGACCTCAAGTGATCCACCCACCTCGGCCTCCCAAAGTGTGG







GGATTACAGGTGTGGGCCACCATGCCCGGCTGCAAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene KRT8. In some embodiments, the sequences from gene KRT8 comprise or consist of:











(SEQ ID NO: 1017)



GTGGGTGTGGGTACCTCTGACCGGACCTGCTTCCCTATCCCTGGG







ACCTGGGGTGGGGACGGTGGGAGCCCCCTGAAGCCCCTTGGGACT







TGGGGTCCTGTTGTTCTGGGCCAAGAAGGGCTAGGAGTTGGTCCT







GACACCCCATTTGTCTGGGTACAGGCTGGAGTCTGGGATGCAGAA







CATGAGTATTCATACGAAGACCACCAGCGGCTATGCAGGTGGGTG







TCCCAGGGCCCTGGGATGAGGGCGGGAGGCAGGGCCCAGGGAGGC







CTCAGCTCCAGGGAGGGGGCTGTGCTCAGTCGCTCACAGTGACCT







CAGCCTGAGCACTCATGTTCTTGGGAGAATCCTAGGGTGGGGAGG







CACATATTCAGGACCCCAGTAATAACTTTATTACTTAGTAACTTT







ACTATTAGAAAGCACCCAAATAAACCATAGCTGTGTGCCAGGCAC







TGTGCTAAGTATCCTACAGGTTTTATGTGATTTATTTTATTTATT







AATT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene KRT8. In some embodiments, the sequences from gene KRT8 comprise or consist of:











(SEQ ID NO: 1018)



AAGGGTGTGGAGGGGAAGCACCTGGAAACTGCTCATGTCTCCCTG







ATCTGCTTCCTTAGTCTCGTTTATTTATTTATTTATTTTTGAGAC







AGAGTCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATCT







CGGCTCACTGCAAGCTCCGCCTCCTGGGTTCACACTATTCTCCTG







ACTCAGCCTCCTGAGTAGCTGGGACTACAGGCGCCCGCCACCAGG







CTGGCTAATTTTTTTTGTATTTTTACTAGAGACGGGGTTTCACTG







TGTTAGCCAGGATGGCCTCGATCTCCTGACCTTGTGATCTGCCCG







CCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGTG







CCCGGCCCTTAGTCTCATTTAATTTGAGCTGGGGGGAGTCAGCCT







AGGTGGTGGTGGGGAGGACCCTGAGGGAGGGTGGGAGCACGGGAG







AAGAGAAGGCATACCCAACCTGACCTACTTACCTGTCCCCTACCC







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CSAD. In some embodiments, the sequences from gene CSAD comprise or consist of:











(SEQ ID NO: 1019)



GTGCATACAGTCCCCTTCTTCCCAAATCCCGCCCTTCAAACCATT







GGTCCAGAGAATCATCATGGACTAGGGAGTTTAACATGGCTGGGG







TAGGAGGCTGAGGGGAGGGGGACTGGAGAGAGGATTGATTGGAGC







TGGGAGGTCAGACATGGCAGTTCTTCCTGATGCTGGGGTGAGGGT







GAGACCAAAGCCCCTTTGCTGGGGACCTGGAAAAGGAGGAAGGGC







TCTGGTTGGGCAGCTGGAGGAGGAGCCACTGACCTGCCTTTGCTC







CACCCCATCCAGGGCTGACTCTGTGGCCTGGAATCCCCACAAGCT







CCTCGCAGCAGGCCTGCAATGCTCTGCACTTCTTCTCCAGGATAC







CTCGGTGGGTCTGCCCCTGCCCCTGCACCAGCCCCACCTCTCTCT







GGAGCTCTGCCTTCTCTATCCCACCTTAGATAAAGTTCAGGCCTT







TCTCTATTCTCTTTACCATCCCTTCTGAAGCTGCATCTCAGCTAC







CACC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CSAD. In some embodiments, the sequences from gene CSAD comprise or consist of:











(SEQ ID NO: 1020)



CACTATGCCTGACTCAATTTTTTTTCAATAGCATTGGATCTTCCA







GTAAAACATTCAAGTTCTTTGAGAGTATGAGCTTTGGAAATGAAA







ACCTAGGTTCGAAGTCCATCTCTGCAGCTTCTGCTTGGATCTTTG







AATCTCAGTGTCCCTGGCTGCACATTAAAAAGGACAGTAACACAT







TGTTGTGAGGTTGAAATGGGCCATTTTAGGCATGCAAAGAGCTTA







GAGCTGGGCCCAGCATCAGGCTCTGAGTAGCTTTTGAGATGACTT







TACATCTTTCTCCCTCTACATTTTGGCAACAGGGTGCTGTGCCGT







TCCTGGTCAGTGCCACCTCTGGCACCACTGTGCTAGGGGCCTTTG







ACCCCCTGGAGGCAATTGCTGATGTGTGCCAGCGTCATGGGCTAT







GGCTGCATGTGGATGTGAGTGGGACTTCGGCTTGGGGGTGCGGTG







GGGTGGGCTGAGCCAAGGCCAAGGCCCACGTTGTTCCTCCTGCTC







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene RDH16. In some embodiments, the sequences from gene RDH16 comprise or consist of:











(SEQ ID NO: 1021)



GTAACCACCCAGGTTTGTCAAGTCTGTCTGTGTCTTCCCTCTGTG







TGAAGGGACCACCTCCTGTCTGCTCCCTGGGAAGATTCCTACAGT







GTCTTTCCAGGTTGAAGTAACAGTAGCTTTCTAATATTTCTCACT







TAACAGGCTGCCTGAACCCCATCTCTTTTCAATCCTTAACCTCTC







CCGACATTGGAGGTGTCTAGTCTTGGGGAGAGTTTGCCCTGATGA







GATTTTCTCTCCAGAATTCCTCTCTATCCTCTCCCTCCTGTCTAA







GCAGAAGTCATGTGTTGTTTGATGCTAAGAGCCTGGGTTAGAGAT







CTGGAGACTTGCTGGAGCATCGTTGAGAAGGGAGCACAGGGCTGC







AAGTGTGCACGGAGGCTGTGCCTGTGCTGGAGCCAGGCTTATGTT







CCCAGCACTTTACCTTGTTTTTGCTAGGGCCCATCAGCGTCCACT







GAGAGCTACGATCATCTCCATCTTCAGAGGAGAGTATCAGCTTAA







GGAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene RDH16. In some embodiments, the sequences from gene RDH16 comprise or consist of:











(SEQ ID NO: 1022)



TTCTCATCCTAGACAGCCTGACCGTGCCCTAGTTTTCAGAGGAGT







GATTGATTCTGGGGTGCGGAGCAGGATGAGGAAACTCAGGCTCAG







AGAGGGATTTCCACCTAATGTGCCTGGAAACCATGGGTAGCTACC







CAATAAGGCCTTTCTATGTTCAGAAGGTCATGGACCAGGAGTGTT







GGTATCGGAGCTTTGTAGAGACAGTTTTGACCCCTGTACTTAGAG







CAGAGGGGTGTGTTTGCCATGGAGAGTGTGTGTGGGCAGCTGTGT







GTAAGCCTGTGTGAGTTCTGTGTATGTTCCTGTGTGAATATATGT







GTGTGCTCCTTCATGGACACAGCATGCATGTAGTGGGCATAGCAA







GGCAGAGAGAAATCAGGAGACTAGGGGCACATTGCCCAGTAGGAA







GATAGTATCTTCCTACTGGGCAGGACTTGAGGTGTGCAAAGGTGT







GCATGAGTACTGCAAAAGCAGTTCAAAGAGCAGAGAGGTGGGGCC







AGAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MON2. In some embodiments, the sequences from gene MON2 comprise or consist of:











(SEQ ID NO: 1023)



AAGCAGTCTACCTTACTTTTTTTTTTTTACTATATTGTTCTGAAG







TCAGGTTATTGCAAGAATAATCCATATTTCATATATTTTTAATGT







AGAAGATAATTGACTTATTTGGGGACAGGTGGAAAAGGGCTTTAT







CATCTATTCGTACTGATTTACTTTGCAGATACAAACTGTATAAAT







AACTTTGAATCATTTTTACCATTCTTTCTCATTTGTTTGCCCTTT







CTGTTGTTCATTCCTGTCCAGTCCATTCTTTACATGATAGCCGGA







AATGATTTTTCCATAGTAGGGCTTTGATCATTTATTTCCTCATCC







TCCCAAAGCAAAAAAATATTTTTCATTTACCGTTTCCTACCAAAT







TGCAAAGAATACTCCAGTCTGACTCCATTCTAGTCACACTTTATT







TCCCACAATAGATTTCATTTCCCCTACTCTGCACTAAACTAAAAT







TCTCAATCTTCCCAAATTTTTTCAACCTTTTGTTTATTTCATTAT







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MON2. In some embodiments, the sequences from gene MON2 comprise or consist of:











(SEQ ID NO: 1024)



TAAATAATTTTTCTTTTCATTGAATGCAAAGCTTTTTTATGTGTA







CTTGTCTCGAAGCACTCTATTTGTATTACAATTATGCATGTACAT







GACTCATCTCCCTTTACATTGTTAAGATTTTTGAGGGGCTTTTCA







GACCATGTTTTGTTCTATTTTGAATCCTCTACAACATTGCTTTTC







AAAGTATGGTTTGTTGGACCAGCCATTGATTGGCTTCAACAGTTT







TGTTACTGGTCTGGGACAAGATAGTGAGCTGGCATCAGAATATAA







ATCAGATGTGTCACTAAACACACTGCTTGGTTCAGCTGACTTTTT







TTTATACAAGAAGACATTGGTAAAGAAGAAAACTGTGTGTTGGTT







TACATTCTGGCACAAGCTTCTTATTTTGTAGCAGATTGGCACCTT







AAGGAGCATTACTTTTAATATCCAAGAAAAAAGTAGATTCCTAGT







AAATTACTAATGAATGAGTTGTAGGATATTTGTTCCTGTCAACTA







TTAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene BTG1. In some embodiments, the sequences from gene BTG1 comprise or consist of:











(SEQ ID NO: 1025)



CTTCTGACTGTTATTTATGAGCCTTTTCATGGTATTTATCATTTT







AGTATTGAAGATGCAGTGTTCTTTTGGGAATTACGTATGCTTAGA







AGAGAGTACTGATGCTCAAAGGTGAAATGATTTGAATCTAGAGTT







GGATAAAAAGGGAAAGGATGGGGAAAATGAAAACTGGGAAGCTAA







ACTGAGAAGGAATTGCAAGGTTTGGTGATAAAAATTCATTTGCTA







TGGTCATAGTTGTGTATCGAGAGGTTTGCACAGCACAGTCTCTTG







GCAGAGTTTAGGAATCTCTACTCTGGGGATTCTGCAGTTCATGAA







TCGTCAGGGAGACTCTGCATTCAATTTGTTTCTGTGAACATGCAG







GTGAAAGTGGTAGTACAGGTGGAACTTCATGGGAACTGGTTTTGG







GGGAGGTGTGGATGGAAAGCACAAATGTCTCAGTATTTTCTAAAG







GGAGACTATTTTTAAAGGTGTCTTGTTACAAACCACTTGATTATA







GCCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene BTG1. In some embodiments, the sequences from gene BTG1 comprise or consist of:











(SEQ ID NO: 1026)



CACACCATTCTCGAGAGTTAGAATTCGTAGACCCGCAACACATGG







ACTACCCTTGCCAATTGTTATGGTTTTATTTTTAAACCTATTCTG







TGAAAAAGCTCCTCGGTATTTCAAACTTGAATTACAATAAAAACA







CCAGGTAGGGTTGTTTTTAAATGGGTCTCACCCCAGTGCTGACTA







CTGTTGTTTACCAACTACATTTCATCTGGTAACTCCATTGAAACT







AAGTACCTCAACCATTTTTTTTTTTTATCATAAAGCCTTTTTTCA







AATGGAAAGTTGTTTTTGAAGATCTTGACATCAGACTTTTTCAAT







CTTTGAAAATGATGGAAACAGTTAATAAAAATGTTTATTTTAGAA







TTCAGGTGATGTCTGGCTTGTGAGGAGCTGTTTATAAGTTTGTCT







TTGAAAAAGGCTTGCACACATCACTCTGAGACCAGCTGTTTCCTG







TGACTTTAACCTTACCTTCCCCAGACAATTGAGCTGAACCTGTAT







CTGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene HAL. In some embodiments, the sequences from gene HAL comprise or consist of:











(SEQ ID NO: 1027)



TTATGGTGTCGCTTTATTCTTAGGTATTTATAGCAAAGTTAAGCG







CCTCTAACAGCAGTTAATCAAGCTTCTGAAGGATTCAGTTGACAG







ATGTCAACCGTTTTAAGAACAATATGCTTGACTCAGTACACTCTT







TTTCCCATTGGCTAGCAACAAAAGAGTTGGAGGTATCAGGATTTC







CTCCCCAGCCCCGCAGGCTCACCTGGTCTTTGTCACAAGTTGTCT







CAGGGCAGCTGGAGGAGGCAAACCCTAAAGAACAAGGGCTTTTCA







AGTCCCTGTTGTGCCACGTTCGCTAATGTCCTGTTGGCTAAAGCG







AGTCACGTTCCAGGCCCAGAGTCAATTTGGGAGGGTAACACTCAA







GGGCATGGGCATGTGATTTGACGTGATCCATGGGCCTGTATCTGA







CAATCTACCACATGCATAAAATCCCATCACAGGCTGGGTGCAGTG







GCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGAGTGG







ATCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene HAL. In some embodiments, the sequences from gene HAL comprise or consist of:











(SEQ ID NO: 1028)



AGCTACTCAGGAGGCTGAGGCACAAGAATTGCTTGAACCTGGAAG







GTGGAGGTTGCAGTGAGCTGAGATCATGCTACTGCACTCCAGCCT







GGGCGACAGAGTGAGACTCTGTCTCACAAAAAAAGAAAAAAAACA







AAAAAACAAGAAAAAAACCACATAGGTACTGTTAAGACCCTGGAG







CAACTATCCTTCACTCTATCCACACTGAATTCTCTGGGTTTTGTT







ATTTGCTTGCTTGTTTTGCTTAGGAAAAGTATGCAGCTTAAAATC







TTTATGATGACTTTAGAAAGTTTTGGACTTTGTATATTTACAGTC







TTTAATGCAACCAAAGACTAATTGACACAGGTCATGTGTCAAATG







GCTTAAAATAAAAGCAAGCAAACAGCAGCTGTGTCTGGTCATGTA







TAGAGTATGTTTTGACTTCGGATGAAGTTGATGTTTAATAGCATG







TGGAAACAATGTCGTCAATATTTCAACTTTGTTTTGTCCTTGCTT







TTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene NR1H4. In some embodiments, the sequences from gene NR1H4 comprise or consist of:











(SEQ ID NO: 1029)



ATTTGGGTGTCGAATGATGCCATGCTCATGATTTGTAGAGCATAA







TAACAATGTTAGCCTTAGGCCATTTTTATACATTATCTTGAGGCA







GAAGCTAGTTGTTACTTTGCTTTACCATAAAAATTATGACTTGTG







ACTAAAAAACTATTCAGCAGAACCTTCTAAAGTTATGTATGGATA







TGTCCTATGACCCAGTGATTTCACTTCTGAGAATATACTCAACAG







AAAGGATGCTCACGTCCACCCTACAGGTTATCTTTAGGGGTTTTG







ATTGGAAGGGGAATTGGGAGGCTTCTGGGAAGCATTACATGTGTT







GTATCTTGATCTCGTTATAATTACGTGTGTGTGCATAAAAATTCA







TCAGGTTGAACACTTAATTTTCACATTTTGTGTACAACATTTAAC







TTACACTTCAAAATAGTTAACGTTGCTATAATTATGCTGAATTAA







TGCTTTTCCACTTTTTAATTTTGTCATTTTATTTTAAACTTCAAA







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene NR1H4. In some embodiments, the sequences from gene NR1H4 comprise or consist of:











(SEQ ID NO: 1030)



TTTCTTTGGGAACAAAATTTGATAAATATACAAACTCATTTCTTT







AAATGTTGAAAGACATCCTATTGAATCTGAGGGGGTAATTTACAG







AAAGATACTGTCTCTCGCACTTTTGTCATCTCTGGAAAAGTCCTG







GTGAATAGTCATTCATTTGTAATAGCCCTCTGATCATTTCCTTTT







CTTTGAAGTTCAAAGAGAATAGCAAACAGAAAAGTTAACAATTTT







CACATAAAATATTCCCTCAAGCAATCCTTTCCCCCATAGTTAGTC







TTGAGGCAGGATTCACAAATTCAGAGTATGCAGCTAACTGCAGTG







ATGGGACCTTCGCTTTCCTCTCTTAAAAGTGAGGAAATGATTGGC







TTCATTGTATCCAATCATCTTTCTGTTTTGTTTTGCTTTTGAAAA







CATTCCTACAGCTGGGAGCACAGCTGCTGTATACTTCCCCCAAAG







CAGGAATTAGTTCATTTCCTTCCTTCATTGTTACACTGTTGCATT







CTTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene IGF1. In some embodiments, the sequences from gene IGF1 comprise or consist of:











(SEQ ID NO: 1031)



ACCTTTTAGAGAATCTCGCTAAGAAACATGGAGAAAACGGAAAAG







AAAAATGTAATGCCCTAGAAAGCGCAAAGAAAGACAGTGGCAAAA







ATGAAAAAAAAAAATAAAAATTATAAAAGAGGCAAAAAAAGACAC







ACTATTCTCTGCCTCTAAAACACAATTAAATAAAAGAATTTAAAT







AAAAATTAAGGCTTCTATATGCATTTTTAAATTTTGTATGAATCT







GTTATGGAAGAATTGCCTATGTCAATATATGTTCAGAGTTAAATA







TTAGCCCCAAATGCTCAGCAAGACTGAATTGTGTCATAGAAGTTC







CCAGATTCCCTTTTCCCGCAATGTCATTGGAGGCTGCATTTCTTA







GTCAAGTCCAGGGTTTAGGCCAAAGGGCATCCGGTATTGCCTAAA







ACCCTGTGAGGTCTGTGAGGTAACTTTTGAGAAGAGGTCACTGCA







CTCTTCATCTTTTTTGCACTTTGGAATCAGATATAAAAGATGTAT







AAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene IGF1. In some embodiments, the sequences from gene IGF1 comprise or consist of:











(SEQ ID NO: 1032)



TGGTCTTAAACCTCAGCTGAGGCTGGAAGAGGACCATGGTGGCTT







ATTGGAGATGTGACCCCAGGGAGCCCCTCTGAAGGATGGAAGGGG







ACTGGGCAAGACCCAACACACACAGAACACAGTAGCCACTGGCCA







GGCAGGAAGCAAGGATCTCAGAAAAGACTTTTAGGTGAATGTGGC







AGGAAAGCGTGCTTGCTGGGGCAAAGGCAGATTCATTCTTTCTCT







TCCCAGGTGACCCAGCGCCTCTTGGTTTCTAACTGGGGAGGGGGT







AGGTGTCAAGAGATGAGTCCCAAAGTTCTGGAATGGTGGGTCTTG







TGACTGAGGTCTAGACCCCTCTCCAGCATGAGTGCTGTCTCCTGC







ATCATATGGAGCCTGGGCATTCTGAGCTCATTCAAAGGGACACCA







TGGGAACCACTTGTTCTCAATGCAATTATTTTTGTGATGTTTACA







GTATCAGCCCCCATCTACCAACAAGAACACGAAGTCTCAGAGAAG







GAAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene HPD. In some embodiments, the sequences from gene HPD comprise or consist of:











(SEQ ID NO: 1033)



TACTGTGTCTGTGTTTGTGTTGCGCATAAGGCAGTAGTGCCTTAT







GGCAGCCAGGGGGAGGCCAGGGCGGCCGCCAGCGCCGCGGGACCG







AGGAGCTGCGGGTGCCAGCGCTTTGCCAGCGGGACCACGCGGAGA







GGGGAATTCTGGAACCAGGGAAGGAAGGGGACTTGCTCTGGAGGG







AAAGACGGATGACACTGCTTTTGTAGAGAGATAAACGCGATTCAG







ACAATAAAGCAAGCGATGAGCCGGGCGTGGTGGCTCACACCTGTA







ATTTCAGCGCTTTGGGAGGCAGAGGTGGGAGGATCGCTTGAGGCC







AGGAGTTCGAGCCCAGCCCGGTAACATAGCAAGACCCCGACCCCC







CTGCGTCTACACAAATAACAAATAAAAATTAGCCTGGCGTAGTGG







CGCACACCTGTGGTCCCAGCTACTCAGGAGGCTGAGGTGGGAGGA







TCGATTGATGGACAGAGGTCTAAGCTGCAGCGAGCCCTGATCCTG







ACAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene HPD. In some embodiments, the sequences from gene HPD comprise or consist of:











(SEQ ID NO: 1034)



ACAGCGCAAGGCTCCGTCTCAAAAAAGAAAAAGAAAGTGTCCTGA







TAATGTCTGCGGGGAGGATGGATTGTAAGGATACAAGATTAGAAG







CGGGCAGCAGTCTTGAATGGTGCCTTAGGAACTGTAGTAGGGGGA







TAGTAATAGGACAGTTTGGGGATATATTTGGAGGTGGCTGAACTG







CCAGAAAAAGAGGTCATTTCCAGACTTTTGGGGTGCAGGAGCCCC







TGTGGACTTTCCAAGGATCTCACCGTGCCAGCCTCCCCTAGCAGA







GCCCCCTGTCTCCCGCCTAGGAGCTGAAAATCCTGGTGGACTACG







ACGAGAAAGGCTACCTCCTGCAGATCTTCACCAAACCGGTGCAGG







ACCGGCCCACGCTCTTCCTGGAAGTCATCCAGCGCCACAACCACC







AGGTACTGCTTGTCCCCGGCAGGCCCGAGGGGACAGGCAGCTAGC







ACACTCGGTTTTCTGGGTCCTTGGCTCATCTCCTCTCTTTCCCGC







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PHF11. In some embodiments, the sequences from gene PHF11 comprise or consist of:











(SEQ ID NO: 1035)



CTTCTCCAGTCGTTCCAGTCCTCCCCGGCCCTCTACTTGAGTGGG







TGGGTCTTCTGATGGGTTCCTGCCCTTGCGTGTGTGTATAGCTCC







AAACGGTGTGCCAGGCTCAGGGCAAGGTCCTGAGCATACAAAGAT







GAGTTAGGCAGACCCTGCCCTAGAGACTCTACTAGGCAACTTTAA







TGAGGAGGTCAGAACCACAGAGGTACAGATGAGAGACTCCTGACT







CCCAGTCTTCAGGGAAAGCTTTTTGAGGAGAGGATGGCTGGGCTT







ACCCTTTTTTTTTTTTTTTTTTTTTGAGAGGAGTTTCACTCTTGT







TGCCCAGGCTGGAGTGCAAAGGCGCTATCTCGGCCCACTGCAACC







TCTGCCTCCCGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAG







TGGATGGGATTACAGGCATGTGCCACCACGCCCAGCTAATTTTGT







ATTTTTAGTAGAGAGGGTTTCTCCATGTTTCATGGGCTTACCTTT







AAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PHF11. In some embodiments, the sequences from gene PHF11 comprise or consist of:











(SEQ ID NO: 1036)



TAAGTCACGGGAGATGCGCTTCCATCCTGACCTCTTCCTCAAACT







CCAGACTGGTATAACCAACTTCTTCCATTTCTGAAATGATCTCAA







ACTGAGTATGTCCCAAAGAGAATTCTTGTTTCCTCCACTGTATTC







AGGTACTCCAAACCTGCTCAACCACCAGCCCATTCCATTCGCCCA







GGTGCTCCTTGCAAAAACCTTAGTGGCTCCTCAGGGCCTCTCTTT







CCTCCGGACACCCCTTCTAATGCATCAGTCGGGCCAGCCAGTTCT







GTGTTCAGCCTGACTGTATCTGGAGCCTGACTGCTTCTCACCACT







TCCATTACTGCCACCCAAGCCTACGCCATCATGATCTTCTGTCTG







GACTATTGCTTTCCTGGCTTCTATTCTAGCCTTTAATACAGGCAG







GCCTGCAGAGCGGCCAAAATGCTTTTTGAAAAACAAAAAGCAAAT







GTGATTCTCCTACTCAAAACCCACCAATAGTCTCCCATGACTCTC







AGAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CCNB1IP1. In some embodiments, the sequences from gene CCNB1IP1 comprise or consist of:











(SEQ ID NO: 1037)



TTAAGTATCATTTGTTACTATTATTTTCTCTACTGAGCGTGGAAA







GCTGGCTGAAAATTCCAGAGTGTAATTTCTGTGGGTCTGTTTCTC







TAAAACTTGCCCCTCACGCAGCTTGTACAGAAGTTAGATTGGCGC







TTACTCCCAGCTGGTGGAAGATTCGGAATTAGCTGCGCCTCTGCT







GCGTGAGAGAGGGCTCAGCTTTAAAGCTTCCCAGCTCCTCTGCTT







TCCCGCTGCCCCCTACTCTGCCGGATGTAAAATAGGCCTCTTAAT







CTTAACTGTGGCTGCCAGGTGACATACCTGAGGAAACTCACTCAG







CGGGGCTATTGGGATAAAGGTGTGCGAGTGTTCTTTTCTATCGAC







CTGGAGGGCCAAGGCAACTTTTTTTTTTTTCCTTCTGGGACGGAG







TCCGTCGCCGGCTGGAGTGCAATGGCGCGATCCCGGCTCACTGCA







AGCTCCGCCTCCCGGGTTCAAACGAGTCTCCTGCCTCAGCCTCCT







GAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CCNB1IP1. In some embodiments, the sequences from gene CCNB1IP1 comprise or consist of:











(SEQ ID NO: 1038)



AAAGACGGAGGGTCGCTCTTGTTGCCGTGGCTGGAGTGCAATGGT







GCGATCTCGGCTCACTGCAACCTTTGCCTCGTGGGTTCAAGTGAT







TCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTGCAGGCGTCCAC







CACCACGCCCAGCTAATTTTTGTATTTTAGTAGAGAGAGGGTTTC







ACCGTGTTGGCCACGCTGGTCGGGAACTCCTGACCTCAGGTGATT







TGCCCGCCTCAGCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCC







ACCGCGCCCGGCCCACTATCGCTTAATTTAAAGAGCTGTAATGCA







TGTTTGTAATGCAGACTCAAGGAAAATTATAATTTCCTTAAATGT







GTATAGAGGATGTGTGAGAGTGGAAAGATGTCTATTCCAAGGTGG







ATAAAAAATAAAATGGTGGACTACCTCAACATGGAGGTGCCAGCT







GTCCAATTACAGCTGTCACTCTGTTTCTGGGAATTTCTCCCGATT







CTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PSMB5. In some embodiments, the sequences from gene PSMB5 comprise or consist of:











(SEQ ID NO: 1039)



AGGACTACTTGCATGACCAAAGCCAAGATGGCAGGTACGTCCTTT







TAGAATTTAAGGTTAGTGAAGCCATCTATTTCCCCGACCCCCTTC







AGTGAAAATGGTCTTTCGCATCTGGCCCTTCTTTTTGGAGGCGTG







CTTGCCAGCAGTCAAAATGGCTCCATTCCGGAATAGATTTAATAG







GAAGTGAAGCTGTGACGGCGAGGCGTTGCCCGGCCTATCTTTGCT







AGGCGTTCTCAGAATTAGTTCTTTCTGCCCACACTAGACATGGCG







CTTGCCAGCGTGTTGGAGAGACCGCTACCGGTGAACCAGCGCGGG







TTTTTCGGACTTGGGGGTCGTGCAGATCTGCTGGATCTAGGTCCA







GGGAGTCTCAGTGATGGTCTGAGCCTGGCCGCGCCAGGCTGGGGT







GTCCCAGAAGAGCCAGGAATCGAAATGCTTCATGGAACAACCACC







CTGGCCTTCAAGGTGTGGAGCCAGCCCCCTTGCCAGGCTGAGTAC







TGAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PSMB5. In some embodiments, the sequences from gene PSMB5 comprise or consist of:











(SEQ ID NO: 1040)



TCTCAACCGTCCTCCCGTCTCTGAGATGGTAAATTACTACTGACA







CTTATTCAGGTCTGAACTGAGAATCTAGTTTGTGGATTAACTGGA







CCCTTTGCTTTTTCTTCTTCCATCTGCCTTTTAGGCAATTCATTA







CTTATTCGAATTTCCACCAGAAGGTAGGAGTCAAGAGAACGAATA







TTGGATATTAATAACCGGTTACTGTTAAAGAAGGGTTGCTGTATG







GCGACTGACAACAAGAGGTATAGCACTTTTATTTTGTTGCGTTGT







TTTACCACTAATTGCTGTGGTTTAACTAATATAGGGAAATGGCCT







CAGAATGGATCATTATTTTACCCAATTTTAGAAGGTGCCCTTAAA







CAAGATGGCAACCTCAGGCGCGTTAACAAGGTGCTGAAGCAGCAG







CGATGTAACCTGTTGCTTGGACACCAGGCTGGAGTGGGAGGACTC







ACCGCTAAGGGTTCAGAACCGATTTTCAACCAGTCACGCCCTTCT







CAAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CTAGE5. In some embodiments, the sequences from gene CTAGE5 comprise or consist of:











(SEQ ID NO: 1041)



AAGTCCGGACGTGGTTGGCAGGGCGCACCGGCGCGTGCCCCGCAG







GCCATTTTTCTGACTGGAATGACGGCGGCGGCTGGCTTCTCGGGG







CTGCCGGGGTCTCCCGGGGTACGCCGCCGTCAGGACTTCTGCAGG







GCGCAGACAGCAGGCTCCAGCGAGGCTGCGGAAGGAAAGCCTTGG







GTGTCGGGGCTGGGCCTGGCGTCGGCCCCTGGGTCGGGGGGGTGG







GGGTTGCGCCGAGACGCCTCTAGGAGAAGTCTCGCCGCCGCCGCC







ACCGCGGCCGCCCGAGGCGGGTGCCCTTGGGACATAGCCCTGGTC







TTTGGGGTTGTGCGGCTCACACGACAGCGCGGAGTATGAGGCCGA







ATCTCATCCTCTAGTCCCAAGCCTCTCCACTACCAGGGCTGGGTG







GCTTCGCCAGGTAGAGCGCCGGCCCCTTTAAGAGCAAACGACCCG







GACACGTCTGCGAAGCTTGCGCGAAGAAGGGGAAGTTTGCGGCTG







TCCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene CTAGE5. In some embodiments, the sequences from gene CTAGE5 comprise or consist of:











(SEQ ID NO: 1042)



TGAGCCCAGGCGCGATGAGTGTTACGTGGCCCAGGGGTCGCGGGA







GCCGCCGGGGGAAGGAAGCAGTAGACCGGCTTTGGGGTCTAAGCC







GCCGTGGTCAGAGGTCCCGAAGCCAGTCCTCGTCTGCTGCCCGGC







TCCCGCCCGTGTTCGAGGCAGTAGTTAGGCTCGTAGGGCGCTTGA







GTGGTTTTTGTGTGATGGAGGAGGACCTGGGGCTCTGGGAAGGTG







AAGAAAGCACTTGCTTAGCTGGACTTTGCAAAGATGTAAATGTTT







CCCGATTCCTAAAACTAATTAGCCTTTCCGCTATGTTGTTTATTT







TGAGCAGAGACACGTGGTTCCTTCGACTGTGAGAAACTTGAGGAC







TGTTCCTTTAAATTAGTTACATTTACTGATGTAACTTCTCGTTGA







ACCTTGAAAGAACACCTGAGGTTGGGCGGGGAGTTGAAATTTTGA







TTCTGCATTTTTTTTTTTTTTGGTAAAGAATGAATGCCCTTTTGA







GATG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SERPINA10. In some embodiments, the sequences from gene SERPINA10 comprise or consist of:











(SEQ ID NO: 1043)



GTACTTTGATAATGTTCTGCTCTCCCAAGGCCACAGGGCCCTACG







ATTGTCTCTCCCTTTCCTTTCGTTAGGCCAGCATATGATTAACGC







TACGTGATTTTCTATGAATGTGTTTTCACGTTTCAAAAACAGATT







GATACACATATTGAACAGTGCCAGACGCTGTCATTTGAGGCCCTT







CCCTGGTATCCTATGTGCTTGTAGTCCTTATTATTTTCAGAGCAC







TCTACATAGCTCCCCTCTGACACTTAGAAGCATAGGGTCTTTCCA







AAAAACAGGGGGCTGGGGGATTATCTGGGGGATTTAGGATTGCAT







CATTGCTCCTTCATTTTTACTTTTTGACCAACTCTCTGCCCTTAG







ATTCCTATTATAGAAAATAGGGACACTCCACCTACTACAGTGTTA







GAGGCTAAATGAGACAATGAATGTAAAGTGCCCAGATGGGCTTGG







CACATAGCAGACACTGAGTATCTATTGTTTACTTGTTCTTCCAAA







CTGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SERPINA10. In some embodiments, the sequences from gene SERPINA10 comprise or consist of:











(SEQ ID NO: 1044)



TCCCCCTTCCTTGCCTAACGGGCATGCAGGCACTCCAATCCCCAG







CCAAACAGGGCACTGGGCAAGGCCGGCCACCCATCTGGATGGGCA







GCCTGACGACCAGATGGTCAGGGCAGTGAATGAAGCAGATCAAGG







AAAGGTGTGTGAGGACCCCTGATTCCACCTGCTTGGACCCCCACC







TTCTGTGCTGCCTCCTGCTCCCAGAGTGGACTCTCTTGCCCTGGC







CCTCAGGGAGGAGACGGGATGAATGAAAACGGGGTCAGGACTGAG







AGCTGCCTGCCGGCCTGGCAGGGAATGGGAACTGGAGGAGGTTTT







GCTCTGTGAAATAATGTCCCCTCTTTGGGTGAGCAAATGTCACCC







ACACTTGCTCTAGGTCTCCCTGGGGCAGGGCTAACCTACTTGAGC







CACAGGAAGGAGGCAGGGTCCCTGAAGAAGCTTTTACTATCCACA







AAGACATTTTAGGAGGCATTAAAACCATCTCTATCCTCTCCTCTC







CACA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SERPINA5. In some embodiments, the sequences from gene SERPINA5 comprise or consist of:











(SEQ ID NO: 1045)



TCCCCTCATTCTTCTGAGCCCCAATTCAGTGATTGGTGCAATCAC







AGGCTTGGCTACAGTGACCCATTCATTGCAGGCATGGTGAGTCTC







TCAATCCCTCTCATTTCCACTAGAATCTAACTGTTGGGATCTATG







ACCCAGTCAGCATAGCAGGCCTGTGGGGAGCTCTCAGGTTCAAGC







ATATGCCCCCCCTAATCTACAAGAAATTAGCTGCAGAAAACCAAG







GAATAGAACCTGGAAAAAGAGAGGGTTTGCTAGAGCTGTCCCTTT







CCCTGTCTCTGGAATGCCAACAATAGGGAGGCTCTTTGGTCTTGT







CTCTCAGGAGTGCCCATGCCATTCCAGGAAAATGATGGCCCAGCT







GGTGGTGTAAGGCTTGGGGGGCAGCGAGTGGGCATCGTGGTGAAA







GCCTCGGGATCAGGGAGCTGCGTCTGCAGGCAGGCCTGCTGGCCG







GAAACCTGCCAGGAAAGGAAGGGGCTGTCCCGGGGCGGGGCCAGG







GAGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SERPINA5. In some embodiments, the sequences from gene SERPINA5 comprise or consist of:











(SEQ ID NO: 1046)



TAAGTACCGATGCCGCAAAGGGAGGTCCCCAGGGCTTGAGGGCAT







GTGAGGCGAGGAGAGGATGGACTCTAGAGTTTTGGGGTTTGGGGT







CTGCAAAGCTCTGAAGGAGTCTCATCTCTGCAGTTTCAGGTATCC







AAGGCAGCAGAGGTGAGTGGGTCCCCCGAGCTCTGTGACCTTATG







CTCCACACTAACTCTGGCAGAGCCTCCGTTTCCTCATAGGTAAGA







TGGAAATAATTACACCCTCTGGATGGTGTGACTGAAGATTAAATA







CAGCGGGTGCTCTCACTCAGCACATCTGGCCATGTCTGCAGACAC







ATTTGGTTGCCACAACTGGAAGGGGGGTGGGGGTTAGTGACATCT







AGAGGCCAGCGATGCTGCTGATGATCCCACAATGCCCAGGACAAG







ATCACAAAGCATCATCCTGTTCAAAAGGTCAACAGGATCAAGGTT







GAGAGACCCTGAAATAAGGCCATGGGGACAAAATGTCGGCTGGAT







AGGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PPP2R5C. In some embodiments, the sequences from gene PPP2R5C comprise or consist of:











(SEQ ID NO: 1047)



AGCGTGGTGGCGGGTGCCTGTAATCCCAGCTACTAGGGAGGTTGA







GGCAGGAGAATCACTTAAACCTGGAGACGGAGGTTGCAGTGAGCC







GAGATCACGCCACTGCACACCAGCCTGGGCAGCAGAGTGAGACTC







TGTCTCAAAAAAAAAAAAAAAAGTGAGCAATATCTGTGGTTAATC







TCAAATAGGGTGGCTTTCCGTGTCATTTCCCTTTAGTTGTTTTTA







AATGCAGATGTTGCTTTAATTAAATAATATTTGAAATAAGTGGGG







TCCCAACTATAACCTTATATCTTATTGTTTTTAAATGTTGATATT







TTGGTGTTTTCAGTAGAATTTGAGCTGTTAACAGTGATTAGGTTT







TTTCAGTTTGTCATCGATGTATGTGACCTCGTTATAATACACATG







GATTTTTGTCTGCTGGTTGTGTTACTCATGAGCAATGTGTTCTAA







TTATGGTATTTTAACTTATAACTTATGAAAAAGTTATGTTTCACT







CTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PPP2R5C. In some embodiments, the sequences from gene PPP2R5C comprise or consist of:











(SEQ ID NO: 1048)



TGGGTGGTGAGGGTGTCTGTATATGTGTGTTTACATTTGTAGACA







GTTTCTGTACATGTATAATTAGGAATGGGTTGTCGGTTTTGGTTT







TTTTCCCAGCTTTATGTAATTGGTCAGACTTCTCAAAGGGGGGTA







GCACTTGATAATCGCTCTCTTGGAACTCTGAAGCCTTTGCATGGA







GCAGTGTTCACCCTGGGCAGCCTGTGGCCCCTGGGGTCACAGAGC







TGCTTTCCAGGGGGTTTGCAAGGCCGTCACTGCCTCATAAGATTA







CCAAGATCTAATTTACCTCTCACTGTGTGGACGTTTGCCCTGGTG







GTGCAAAGGCAATGGTGAGGAATATGGCGCCTTTCTTTCATCGAG







CAGGACAGCGGTGTTCAGCTAGCAACACACCAGTCTGACCCAAGA







ATGTCCTCGATAAAGCATGAAGTTACTGGTTTTTTAAGTCTCAAC







TCTTTTGTATACCTTTTTAATTTTGTTTTTGTTTGGGTTTGTTTT







TAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SERF2. In some embodiments, the sequences from gene SERF2 comprise or consist of:











(SEQ ID NO: 1049)



AGGATCCGGCGTCCGGAGCCCGGGATCACCTGCCTAGCGGGCTTC







ACTTGGAGAGGTGGGGAGGCCAGAGCTTGCGGAAGCCGCTCCCTC







TCCCCTGCACCTTCTCCAGGGTCGCCGGCTGAGGCCCAAGGTCCC







TAGGGGCAGTGACTAGAGAAGAAGGGGGCCGGCCGTACCTGTTGC







CGCTGACACTCTTGCAGCGCCCGCAGGGCAGCGTCGTTGAGCCTG







AGCAGTAAGAGGCTAGTCCGGGCAGCTTGCGTGAAGCAGAGCCGG







AGCTGTCCTCTCAGAGGCTCCTGGAGCTCCTCCATGGCGACGAGT







TCGAGTGCAAGCAGCACGGGGGCCACAGGCGAGGGCCACCACCGC







CACCTCCTCTGTTCAGGGTTTGGTTGGCACACCCGGTAACCCACG







TCTGCCCCACCCTCCGCCAGGAACCCGCCCCCGGCCCCACCTCCC







GCCAGCGCCACCCCAAGCTTTCCCAGCCGCGTGGGCGGCGCTTCG







CTCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SERF2. In some embodiments, the sequences from gene SERF2 comprise or consist of:











(SEQ ID NO: 1050)



TAATTGGGGCCCTGCGGTTGTGGTGGCAGCAGTGAAAGTTGGCTG







TCACCGACTGGAGCGCCCCCAGTTGGTGGGCAGGGATACGGGCGC







GGGTAGTGGCAGGAAAGTTGCAGGGCAGAGAGCCAGAACCCAGTC







CTAGGTCTCACCTCGTGGCCTAAACACGCGTCAGAGTGGTGAGAC







CGCTCCCTGAGGAGGGCCAGGAGCTGCCATGAAGTCGCCAGGCAT







TCCAGAGGGCCAGATCCTGGTGCACTGAGACCCCAGCACCAACCT







CTGGCCAGCCCTTGGTGTCTCCACTTGTTCCTGTTTGATTATGTT







TCCCAAGAGCCCCGATGTTGTCCTGTTTCCTAAACAGGATCCCTC







TGTTAAATCCCTGGACCTGCACAATCCCCGGGAACCTTAACGTCC







TCTACTACAAATAAAATTACCTATACATTTGTCAAAACTCATAAA







AAAAAAATACATTTTTTTTTTTTTTTTTGAGACGAAGTCTTGCTC







TGTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene AQP9. In some embodiments, the sequences from gene AQP9 comprise or consist of:











(SEQ ID NO: 1051)



GCCCGCTGCTGTGGCAAGAATGGCCTGGGGTCAGTGCTAGCTGTG







GCAGTGGCTGCAATACCCCTGGCTCTAAGGGCATCTTCAGTATAC







TGCAACACCCTCAAATTCTGGAGTGGTATTAGGGGTTACTGAGAG







CCCTCTGGGAATTATAAATTATTCCTAGAGCAAGACCAAGCCCCA







CTTTGGCTTACAGTTGACCTCGAAAACTCCAAGAATTCCCCCAAA







AGAGAACTCATTCCCAGGACCCTATCGAAGAATAATTTGTCCAAG







TTTGCAGTGGGCCATTCAGAGAGGCCAAAGGCCTTCTTCCTTTGA







CCCTCCATCCTGGGATTCACCTGGGCATCTCTAAAACTATCATTC







CTCGGAAGTAGAGAGAGACAAATGACATGGCTATGCCAAGCTCAA







TCTGAGTGACTGGTTGTTTAGCACAAGGCTTGGCACAGGCCTCCT







TCAACTCTTCCCAGGGAACACTAATGTGCCAGAGCTTTCTCTTTC







ATAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene AQP9. In some embodiments, the sequences from gene AQP9 comprise or consist of:











(SEQ ID NO: 1052)



TAAGTGTAGATTCAACAAAGACTTAACTTTGGTGAAAAGATATGC







TCTCATAAGGGGAATGCATTGGAGAATTAGAGACCAATCAGAAAG







GAGAGATTATATTTCCAAAGGCAGAGGTTGCTGGGCATAACCCAA







GCTTTCTTGATAATCGTTTTACCTTTACAAGGAGCTGATACCACC







AGAAAAGTTTTCTCCTTCCTCTTTAATACGTACACTTGTTTAACA







TTAGTTGAGATCCAGAAAAGAGTGAATGACTAAACATTTCAATCC







CTGTAGACACAAATGGACTTCACTTCACTATTGACTCTTTTGAAC







AAGTGGTCTAAGTGTGGAGGCCACAAGAGAAGATATTTCTATTTC







AAATCAAAGACTGTGAAGGGTGGTAGTTGAGCCTAGGGGATCATC







AATCCTCTCTGTCTCACCATGTGCATAGGTTTATTTATTTAAACT







CTCAAGGTGGCTAGAAGTATCTCTGCTTATTGACCTTTAAACAAG







CTTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ANPEP. In some embodiments, the sequences from gene ANPEP comprise or consist of:











(SEQ ID NO: 1053)



GTAATCCAGCCCTGCCTTATGGGCAGCAGCCCCCACCCCATACTC







CTAGCTCCTGGCCCATGTTCTGAGGGCTGTGATGGGCCATTCCAT







TCAACAAATGGACTTGACTTCTTTGGTTCCAATCTCCACTTTGCT







GCTGCTTCGCTGTGTGACTCTGGGCAAGTTACCATCCCGCTCTGG







TCTCTAGTTCCTCTTCTATAAAATGAGGCTAAGAGCAATACTCCA







TTAGTTATGTGAGGATTAAAGGAGAGAATCCTAGGCCAGGCATGG







TGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGACC







TGGGGTCAGGAGTTCGAGACCAGCCTGACAAACATGGAGAAACCC







CATTTCTACTAAAAATACAAAATTAGCCAGGTGTGGTGGTGCATG







CCTGTAATCCCAGCTACTCTGGAGGCTGGGGCAGGAGAATTGCTT







GAACCTGGGAGGCAGAGGTTGCAGTGAGCCAAGATCGCACCATTG







CACT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ANPEP. In some embodiments, the sequences from gene ANPEP comprise or consist of:











(SEQ ID NO: 1054)



CGAGAATCTGCAGGAGATGCCCTGGATGGCAGGGACTGGAGGGTC







CCACTGATGGCCACGCCCTCTCGCTCCACCCACAGGCTGTGAACA







ACCGGTCCATCCAACTCCCCACCACCGTGCGGGACATCATGAACC







GCTGGACCCTGCAGATGGGCTTCCCGGTCATCACGGTGGATACCA







GCACGGGGACCCTTTCCCAGGAGCACTTCCTCCTTGACCCCGATT







CCAATGTTACCCGCCCCTCAGAATTCAAGTGAGCAGATGGGGTCA







CTGAGGCATGGTCAGGTGGGTGGGGTAGAGGGCAGTTACTAAGGG







ACCATAGTAGGACTGGCCTCCTGTGGTCCCAGCCCCAGCCACTGT







AGACAGACCCTCCCTCCAGAGCCAGGAGTTCAGGGGCACTCGGTG







CCAGAGCTGGGCAGAAGCTGGGAGCCTGGGCAGCTGCTCCTCACT







GGCAGTGACCACACCGCCATCTCTCACCACCTCTGGATCTTTAAT







TGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PCSK6. In some embodiments, the sequences from gene PCSK6 comprise or consist of:











(SEQ ID NO: 1055)



ATGAAAATGAAAATGACAGATCTGAGGAAGAGGGAGAGCTCTCTT







GAAACCCCTTTCCCAGATTTGCCCTCAGTTTTAGGAATGAGGTAC







GCTTGGGCATTCCTGTTGCCTGCATGGTTCGGCATTCTGGCAGCC







AGGTTCGCCCCAGAATAGATAAGTGTGTTTGATTTTCGAACATTT







GACTTTATTACCTATTTCCAGAAACACTTCGTGTTCAAACTACGT







CTCTACAGGCATTTCTACTTGGTCTTTTTCTCCCCTGACATTTCA







CAGAGTCCTAACTGTGCTGCATGTATAACTTGTTCTTTCTTTTTG







TTACTTGCATAGCAAGCATGTTTCTATGTCATTAAATATCCTTTG







TAAGCAGCATTTTATAGATTTTTATAATACTTCACCAACAGAAGC







ACCATAATTTATTTAAGCATCTCTCTATTGTTGGACATGTAGATT







GCTTTCCATTTTTTCCTATTATGAGCGGACAGCTTAGTACAAAAA







GCCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PCSK6. In some embodiments, the sequences from gene PCSK6 comprise or consist of:











(SEQ ID NO: 1056)



CACTTCCTTGCCTCGGCTCCCCCAGCCTCCCCCACGGTGAAGCCT







GGGCGTTGGAATGGCTGCTGACGTTCCCACCGGGGAGGAGCAGCG







GAGGCGCAGGGGCCGGTCCGACCCAGCGGGACCCCGGGGGGGCGG







GGGTTGTGGCTTTTCCCTCCACTTCCAGGTGAGGCTTGGTATCAG







GCCTCCCCAGCATCTGGCTGCCTGGAGGGGGCAGGTAACACAACC







CAGAAGGAAGGTTTACCCAAGGAAAGACAGGACATAAAGTGTATG







CCCTTCCTGTCTGAGGGCACATTGGTCTCACAAGGCCTGTTTGGA







AGACTTCCCCCAGCTAAGCAACCACCCCGTTTCTTTGTGGAGCTG







CAGCCAGCTTGAAACACAGCTCCTTCCATTTGTCAACCCCGGGCG







CTTCCTCCTGCTGCCCTCCACTCCTGTTGCCCTGGGATTGCACCT







CCCAATAAAGCTTCAGCCCATGGGCTTCACTTCAGTCCCTTGTTT







TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ACSM5. In some embodiments, the sequences from gene ACSM5 comprise or consist of:









(SEQ ID NO: 1057)


GTTAACTATGGAAAGAAAAGAAGAAAGACAGGCAAGAAAGAGGGAAGAA





AATAGGAAGAAAATGAAGAAGGAAGGGAGGGAGGAAATAAAGGAAAGAG





AAAGGAAAAGAAGGAGGAAAGGAAGGGAAAAAGATGAATGGAAGGAAGG





AAATAGGGAACAAAGAAGAAGGGAGGAGAGAGGAAAATGGAGAATGAAA





TAACGGGGAAAAGAATGAAAAAAAGGAGGAAAGAAAATGAAGGAGGGAA





GGAAGAAAACAGGAAAGGGGAAAGAAAGGAAAGAAAGAGGAAAGGAAGG





AGTGAGCAAGGAAGAAAAAAGAAAAGAAAGAAGGGAGGAAGAATGGAGA





GAAATGGAGAGAGGAGAAGGGAAAGAAAGAAGAAAGTGAGGGAGGAGAA





AAAAGAGGATGAGAGAAAGAGTTATCAGGCAGGGTGAGAGAAAGGCATG





GCCCAGGAAAGAAGCTAAGTGGAAAGGCTCTTCTTTATCTGTACTGCGT





TCTCCCCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ACSM5. In some embodiments, the sequences from gene ACSM5 comprise or consist of:









(SEQ ID NO: 1058)


TAGCAGCCTCCCCCAACTTCTGGCAAGGCCTGGCATGGAGAGGAGAAAG





ACACACAGGCCTGGTTGGCACGGGCTGCTGGGCTGCTGGGAGAGGCCCA





GGGTGTAGACACTCACGTATGCACCTCTGTGATGATTTCCTTACCAGAT





TGTGGATGATGAGGGCAACGTCCTGCCTCCTGGAGAAGAGGGGAATGTT





GCCGTCCGTATCAGACCCACTCGGCCCTTCTGTTTCTTCAATTGCTATT





TGGTAAGAGACGGGGAACAGCCGTTCTCATGACAGTGACTGTGTGCTAA





GCATTGTGCACCACACTTTGTAAATATCTATTTGTTTAATCCTTACAAT





GACTGCATGAGGTAGGTTGCTACTATTATTAATAAAGTTCTGTTATTGA





TAAGGAAGACTATTATTGATAAGGAAGCTACTACAAAGGGGAAGTGACT





TATCCAGCATCATGCAAATAGTACAGGCAGGACATGGGATTTAAATGTG





GGTGTGTCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ACSM3. In some embodiments, the sequences from gene ACSM3 comprise or consist of:









(SEQ ID NO: 1059)


GCTGTGGATCAGAGGACCCTGTGCCAGATCATACAACTCCTTGCAGTTC





ATGTAAGGACTCGGATTTTACCTGGAGTGGAAAAAGAAGCACTGAAAGA





TTTGAGCAGGGGAGTAACCTGATAGCGTTTATGTTTAGTCCTGCCACTT





CGACAGATAAACGCACCAATGGGCTTGATGAGATTTAGGCCAACCCATA





ACCGCCCCTCAACTTCTTTCCTTTCAATTTCAAAACTCCTCTATGGCTT





CCTCCATCTGTTCTTCCTTCTGAGAAGTGCTCTCTCTGCCCCTTTACAG





AACTAACCACTTCGGCAACTCCTTGGACACTTTCCTTCTTGTTAATAAT





TTGCTTTCTCCGCCCCTCAAAAGCTTGCTGTTTCTGTAAATCATTACCT





GTAAGAGGAACCGCTGGGAGTCCTGTAAACTTTAGCCCAGAGCTTGGCT





CCTCCTCCAGAATGTCTCCACCAATCAAGGAAAGTGTTTTGGGCCAGTC





TTGCTCCTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ACSM3. In some embodiments, the sequences from gene ACSM3 comprise or consist of:









(SEQ ID NO: 1060)


TGAGTACCTCATCTCCCCTCCTGTGTAAGATTTCCTGAACTGGCACATC





TGTTTTTTGAGCAAAGATAACAAACAGATGAACAAAACCAACAATCAAA





AATGCTGTCATTAAAGTCTTGGGCAGCCAAAGTTTCTCTCAGAATTTCT





CAGTTGTGTGATACTATCTATTAAGTGATGAGGAGTATGCACACACAAA





AGGCTATAAATGTAGCAGCTGAGTTTTCATGTTGAGCCTTTTGGTGCTA





TTTGATTTTTTGAAAAACTATGTACATGTATTAAGTTGATAAATTTTTT





TTTTAATTTTAATTGAACCAGATGCGGTGGCTCAAGCCTGTAATCCCAC





CACTTTAGGAGGCTATGGTGGGCAGATGCAGATCACTTGAGGCCAGGAG





TTCGAGACCAGCTTGGCCAACATGGTGAAACCCTATCTCTACTGAAAAT





AAAAAATTAGCCAGGTGTGGTGGCAGGTGCCTGTAACCCCAGCTACTTG





GGAGCCTGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene TMED6. In some embodiments, the sequences from gene TMED6 comprise or consist of:









(SEQ ID NO: 1061)


GTCTGTTTTCATTACCATGGCTATTATAATAATCATAGGGTCCTATGAA





ATTACAGAAATAATGAGGAAGTATACAGAGATGTTTTAACAGTAGGAAA





AAAATTCAGCCTTTAACACCAAAAACCAGTGTTCCTGGAGGCTGCTGAC





CTCCAAGGATGCCAAGTGGCAGTCTACTGACGATTTGGGGGCAACACTC





CAGGTGAGCATTCTAAGTCTAGGGTTCAGCCGCTCAATTTTATCTCATC





ATCTCCAGTTAATCAGATTCTGGGAAAGTGACCAAAAAACAGAGACCCA





TTTATAATACAGAATTCCAATTAACAAATGGATTACAGGGAAGCATGTC





TTTCCAGTCATCAGAATATTTGAGTCAGTTACTAAAAGCAGATTCAGAA





CAAAAGAAAATGCATGATATCCATGTGAGATATCCACACTCCACAGCAA





AGCACTCCTCCAGCCAGGTCAAGCCTGTCCCACTTTGGAGAGCAGCAGT





GTCTTAACT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene TMED6. In some embodiments, the sequences from gene TMED6 comprise or consist of:









(SEQ ID NO: 1062)


TATAGGCGCACGCCACCATGCCAGGCTAATTTTTGTATTTTTTAAGTAG





AGACAGGGTTTCACCATGTTAGCCAGGCTGGTCTCGAACTCCTGACCTT





GTAATCTGCCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAG





CCACCGCGCCCGGCCCAGAATCATACATTTTAGTGGATCCACACTAGCT





GTGTGTTCCGAGCAAGGCATGTCTTCTTCACACGGGTGCTGTTGGCATT





CTGGGCAGTTCTTTGTCCTGCTGTACTGTTTCTTACATTGCAGACGTTC





AGCAATGCTGTGCTGCCTCCCCTTAACACCATCACTGTGATACCTCAGA





ATCCTCCAATCAACTCCAAACCCCAGCAGGGGGTTGGGCAGTCCCACCC





CAGACAGAGACCGCTGTGCTGATGACCGTGGGACGTCAATCTTACCACT





CAGCTGGCTTCCTCGTGATGTAGCAGGGGTTCTGCTACCTATTTAATGT





GTCTTGGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene HPR. In some embodiments, the sequences from gene HPR comprise or consist of:









(SEQ ID NO: 1063)


AGGGGGCTGGGCGTGATGGCTCACACCTGTAATCTCAACACTTTGGGAG





GCTAAGGCGGGTGGATCACTTGAGGGTGGGAGTTTGAGATCAGCCAGAG





CAACATGGAGAAACCTCGTCTCTACTAAAAATACAAAAAATTAGCCAGG





CATGGTGATATATGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGG





AGAATTGCTTGAACCTCGGAGGTAGAAGTTGTGGTGAGCTGAGATCATG





CCATTGTACTCCAGCCTGGGCAACAGGAGTGAAACTCTGTCTCAAAAAA





AACAAAAAACAAAAAAGAACACCACGGGAAAATCAAGTGTGAAGCAAGA





GCTCAACTCTTAACAGGGGCAATGTTTGGAGTTTTGTTACTGGAAAAGA





TAGAGACCTTACCAGGTCCAAAGTTTGTAGACACAGGAATTACGAAATG





GAGAAGGGGGAGAAGTGAGCTAGTGGCAGCATAAAAAGACCAGCAGATG





CCCCACAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene HPR. In some embodiments, the sequences from gene HPR comprise or consist of:









(SEQ ID NO: 1064)


TGGGTCCACAGCTTTCCCTCCTGCCTTTCCTCTGGTTCTTTATTTCAGT





CTTTTTTGCATACATTGGTACAGATGCAGAAATAGAACAAAGAAACAGG





GCAAATGGGCTAAATTATAGTGAACCAAAGGGCTTAGTGTGTTAAATCT





TCTCCTTTTCTGCATCCATAGAAGACAGTGCTGCTGTCTTTCCCAGGAG





ATAAGATTTACTCTCAGGAGTGTCTTTTTCCTTGAGGTTACGTTTTTGT





CTTTGTAGGGTATGTCATCAGCTCCCGTGGTAGGCTTCCTGGCATTCGG





AATATATTTACTAGCAGATATTTTCCTCTTTAAAAATGTATAATAAGAA





AGACTAATAGTAACACATTTGAATGACACAATTAATTGACTAGTACTTG





GGATACACACTAGTACCTGGGATACATCTAATTAAGACACTTAGATCTT





AAAAAATAAAAAGACTTTTTGAAATGTTGAAATAATAAGACTAGAAACT





TTTTTTTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SERPINF2. In some embodiments, the sequences from gene SERPINF2 comprise or consist of:









(SEQ ID NO: 1065)


TGTCCCCCAGGCTGGAGTGCAGTGGCTTGATCTTGGCTCATGGCAACCT





CTGCCTCCCGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTTGCT





GCGATTACAAGCGCCCGTCACCACAGAAGCCCAGCTCATTTTTGTATTT





TTAGTAGAGATGAGGTTTTGCCATGTTGGTCAGGCTGGTCTTGAACTCC





TGACCTCAGGTGATCTGCCTGCCTCGGCCTCCCAAAGTGCTAGGATTAC





AGGCGTGAGCCCCCATGCCCGGCCACACTGCTAGAAACTTTAAAAGCAT





CTCATTCGGTTACGTGTCCGCCTGCAGTGGGACAGGTAGTATGACTCCA





CCCCACTGTGAGGAAGTGGAGGAGCAGTGGGGTCAGTCACACGCCTGGC





AGGATGGCCAGGAGCCCCATTGTCTGCCTTAGGAGCACCTGCTGGCCCC





ACCCCCACTTAGCTTCGGGGCTTTCTGTCCTCATGCTCTTCCCTTCCCT





TTTCTGTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SERPINF2. In some embodiments, the sequences from gene SERPINF2 comprise or consist of:









(SEQ ID NO: 1066)


TAAGGAGGAGGGTGCGGGCGAGCCCCCGAGGTCAGGCTGGGCAGGGCGG





GTAAGGAGGAAGCGTCTGGCTGGCAGTGGAGCTGAGTCAGAGCTGTCCT





GTGTCCTGCTCTTTTCCAGGGCTTCGGGAGCGGGGAGAGGGTTGAATAT





GAGCCCCCAGACCCTCTGTCCTAGAGGAAGTTGGGGTGGCCCGGGGAAT





GGGGCTCTGCTTTGCTGCCCAAGACCTCGCATTGCAAGGAGAGTCTGGG





TAGAGAGCCTGGGATGGCATGTGAGCCTCGCTGGCTTCCCTGGAAGGGG





AAGGAGGAGAAGCTGGGGTGTCACACTGTTAAAGAAATGCTGGGTGGAG





TGGCTCATGCCTGTAATCCCAGCACGTTGGGAAGCTGAAGTGGGTGGAT





TGCTTGAGCCCGGGAGTCCGACACCAGCCTGTGCAACACATTGAAACTC





CGTCCCTACAAAAAATACAAAAATTAGCCAGGTGTGGTGCATGCTACTG





GGGAGGTTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SLC13A5. In some embodiments, the sequences from gene SLC13A5 comprise or consist of:









(SEQ ID NO: 1067)


CTGTGCTGCTGACTTGTCTCTGGTTTTCTGCAGTGTGGGGAGCCCAGGG





AGGTGGATGAATGAACAGTTAGTTACGCCCTGCCCACCTGCTGGGTGCC





AGGCCTTCCTGTCCCTGTTGAATCCACTAGTTATCTCGTTCGATCTTTG





CAGCAACCCTGTGAGATAGGAAGGTGTTATTATCTTGCTTTGTCTTTCA





AAAAAAAAGCGAGGCTCAGGGAGGCCAAGGGAAGTGTCCAAAGTCACAC





ATCAAGTTACTGGCAGTTACAGTTCCAACCAAGAGCTTCCAACTCCATA





CCCCCTGCTCCTTCTGCTAGCCATGAAGGGCTTTGGCCTTATAGGGCTT





GTAGGGAAAGGTGAGTGGCCAAGAGCAAGTCCATGCCAAGGGAAGATCT





CCAAACATGAGTCCCTGTCTGTTGCCTCCCCTGAGATAGGCACAGGACA





AGTGATCAATGAGACAGGGTGGTCCTTGCCCTAAGAAGCAAAGTGTTTG





GTTGGGGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SLC13A5. In some embodiments, the sequences from gene SLC13A5 comprise or consist of:









(SEQ ID NO: 1068)


CCATGGACTGCAAACTTGACTCCAATCCCCAGTAAAATATCTACAACAG





GGTAGTGAAGCGATGGTTAGTGACCATGAGGGAAGCTTGCAGAGCAGGC





ATCAGAAAGAGCCTGAGGAGGTCCACAGGGAAGCTGGCACGTCCTTGTA





GGATAGTTAAGGCACTGGGGTGAGCAATGAACCTGGACTCACGGAACAC





TGGGCTCTGTGACCGTTTCCCTGAATGGCCTAAGCTGTTGCCTCCTGTC





ACTTCTCTGAGGTCATTTTCCAAATGCGCACGGGCATAGAGAACCCATC





CACTCTGCCTACTTCCCAGGGATGCCTTGAGCACTGAGGATACCTGGGG





GACATGAAGTCGCACTGTCCTGGGGGTCGGGACACCCCAGCCAGGGACA





GAGCATGGCACAGGGACATCGAGGCCCAGTGAGCCGACCCTTTGTCCTC





CTCTCTGAGAGCACTAGTCCCCAGCAGGCCTCAGGGTGCTGACTCTGTC





TCTTTTCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ASGR2. In some embodiments, the sequences from gene ASGR2 comprise or consist of:









(SEQ ID NO: 1069)


GTGAGGCCACGCTTCCCACAGGCAGGAGGAAGCCCTTGGAAGGCCTGCT





CTGGGAGGCTGGATCAGGCTTATTTTATCCATTTTTTTTTTTTTTTTGA





GATGGAGTCTCGCTCTGTCGCCCAAGCTGGAGTGCAGTGGCACGATCTC





GGCTCACTGCAAGCTCCGCCTTCTGGGTTCACGCCATTCTCCTGCCTCG





GCCTCCCAAAGTGCTGGGATTACAGGCACGTGCCACCATGCCCGGCTAA





TTTTTTTGTATTTTTAGTAGAGACGGGGTTTCACCGTCTTAGCCAGGCT





GGTCTGGAACTCCTGACCTCATGATCCGCCCACCTCCGCCTCCCAAAGT





GGTGGGATTACAGGCGTGAACCACCGCGTCTGGCTGAACTTCAAAGTAT





TTTAATGCCACAGTAGTTAAGCCATATGATACAGGGGCCAGAATATATG





TATTGTTTGCATTACATCAGGCTCTGTTAGTACTTTACAGCATACTGTG





AGTACGAAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ASGR2. In some embodiments, the sequences from gene ASGR2 comprise or consist of:









(SEQ ID NO: 1070)


TGTGTAGTGTGGTGTATATGTGTGTGTTGTGTGTGTATGCATGTGTGGT





GGTGTGTGTGAGATGAGTCTGTGTGTGTGCGGTATGGTATATGTGTGTG





TACGCATGTGATGTGTGTGGTGTGTCTCTTGGTGTGTTTGTGTGTGTGT





GATGGATCTGTGTGTGGTGTGGTCTATGTGTGGTGTACATGTCTGTGGT





GTGTGTGTGGTATGGTGTGTGGTGTGGTCTATGCATGGTGTACGTGTCT





GTGGGTGTATATGCTTCCCCAGCCACAGGCTGGAGCCTAGCAGGTGGGG





ACCACACCTGCCGGCAGCACTTTCAGCCTGTCTGTCCTAGATCACGATG





CCCTGCTCTTCCATCTGAAGCACTTCCCCGTGGACCTGCGCTTCGTGGC





CTGCCAGATGGAGCTCCTCCACAGCAACGGTAGGGGTGGGGCCGTCTCC





AGGCCCAGTGTGCCTGCCCCTCCGGCTGGATCTCATCTCCCTGCCCCCT





GTCCCCTCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene TMEM220. In some embodiments, the sequences from gene TMEM220 comprise or consist of:









(SEQ ID NO: 1071)


GTCAGGCGGCCGCGGTGGGAGGGGGACCCGCCCGGCTCCCAGCTCCGCC





CCCTGGCCTAGCGGGCCGTGTAACTGACTAGCGGGCAGAGTCGCAGGGG





CGCACAGTTAGTGGGGAGGACGGGACTTGGGCTTTCTGCTTGGGGAGCC





CCCACTTTCTCCCCCGTCTAAGAGACGCGGATGTGTTCATGGAAGCGAG





TCCCATACCAGGGCTCACACCCTGGAAGGGGCTGGTTCCGCGCGTGGAA





CTTCCCAGGGGACCTCAGAAGAAGCGGCCCTGGCTCCTGCGTTAACGTC





CGGCCCGCGGACGACACTGAACATATCTCAGGGCCTAGGAGACGAAGCC





GACCAAAACCCTTTATAGCATCATTAAACTTTTTAGTCGGCTTGGGGAC





CCCCTTCATAATCATTTCCCTCATTTCTTGAGACTGGTGGGGGTGGAGG





GCCCTTAAAATACCCGTTCTGGGTTGGCGAGTCCTCTCCAGAGCTCCCG





TGAAGGCTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene TMEM220. In some embodiments, the sequences from gene TMEM220 comprise or consist of:











(SEQ ID NO: 1072)



TGAGCCGTATAAGATGTTAATATAACTGTCGTCACTCTAGAAATT







ACCATAATGCTTACAGGGATTTGAAAGTTCCTGAGCTTTGGAAGG







AAATGCATAAATACTAAACAGAACACACACACACACACACACACA







CACACACACACACACACACACATCTCTTTTTCTTTCTCTTTCTTA







TTTTTGTAAAAACAATTTTTATCCTTTTTTTTTTGAGGAGTCTCG







CTTTGTTGCCCAGGCTGGAGTGCAGTGGCACAATCTGGGCTCACT







GCAACCTCCGCCGCCCGGGTTCAAGCAATTCTCCTGCCTCAGCCT







CCTGAGTAGCTGGGATTACAGGCTCCCGCCACCACGCCCGACTAA







TTTTTTCGTATTTTTAGTAGAGATACGGGGTTTCACCATGTTGGC







CAGGCTGCTTTCGAACTCCTGACCTCAGGTGATCCGCCCACCTCG







GCCTCCCACAGTGCTGGGATTACAGGCGTGAGCCACTGCGCCCAG







CCCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene NCOR1. In some embodiments, the sequences from gene NCOR1 comprise or consist of:











(SEQ ID NO: 1073)



TCTGAATTTTCAGAGTGAGAATTGTTGTCCTGTCCTTGAAGCAAA







TCACCTTCAAGTATTACAGTGTAATATTCACATTCTCTGGTAGAT







GAGAGACACAACTTTGTGTAGTGGATATTTCCAATCAGACGCTGG







CTAAGCCTTCACCTTGGCATTTGCCAGCTGTGTGGCCACAGGCAA







GTCACTTAACCTTTCATAGCTTTAGTTTTCTCATCTGTAAAATGA







AAATAATAGTACACGTCTACCTTGCTGTGGTTGTAAAGTTAAGGA







AGGAGCATTCGGTATTGTTATATAACAGGTGGCTCTGCATTATTT







TATGTATAATGTAGCACGTTATTACATATTAATTAGTGTATTAGT







ACTGTATATACACAGTAGATACTATGGAGGTACCTGTTTTCATTG







TTACATCACTGTTTTTATTTCAAGTGATGGATTTGAGTTGGTCCG







TTGCTGTTTTAGTTGGTGTGTGATACACTTTTACTTTCTTTAAAA







AAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene NCOR1. In some embodiments, the sequences from gene NCOR1 comprise or consist of:











(SEQ ID NO: 1074)



GGCATGCCTCACTGCACCCAGCTAAATTTTTGTATTTTTGGTACA







AAGATACAAAATACCAGCCAGGCTGGTCTTGAACTCCCGACCTCA







GGTGATCTGCCTGCCTCGGCCTCCCAAAGTGATGGGATTACAGGC







ATGAGCCACTGCAATGCCTTAAGCATTATTCTTGAAGATAACTTT







GATTAAAAAATGATAAAATCAATTCAACCAATAACATGACAGAAA







TAAGCATTACATTACTTGTTAAGAGATGGCAATTATTACATTGGT







TGTTTGCATATTTCGATTTATTTTCTTCCTATAGGTATCCTGCAC







CCCATGTAACATACCAATTGGAACCCCAGTGAGCGGCTATGCTCT







CTACCAGCGACACATTAAAGCAATGCATGAGTCAGCACTCCTGGA







GGAGCAGCGGCAGAGACAAGAACAGATAGATTTGGAATGTAGAAG







TTCTACAAGTCCATGTGGCACATCCAAGAGTCCAAACAGAGAGTG







GGAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene UBB. In some embodiments, the sequences from gene UBB comprise or consist of:











(SEQ ID NO: 1075)



TAAACTGCCTCTCGGGAGGTTGGGCGCGGCGAACTACTTTCAGCG







GCGCACGGAGACGGCGTCTACGTGAGGGGTGATAAGTGACGCAAC







ACTCGTTGCATAAATTTGCGCTCCGCCAGCCCGGAGCATTTAGGG







GCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGG







ACGTGGTTGGTGATTGGCAGGATCCTGGTATCCGCTAACAGGTAC







TGGCCCGCAGCCGTAACGACCTTGGGGGGGTGTGAGAGGGGGGAA







TGGGTGAGGTCAAGGTGGAGGCTTCTTGGGGTTGGGTGGGCCGCT







GAGGGGAGGGCGTGGGGGAGGGGAGGGCGAGGTGACGCGGCGCTG







GGCCTTTCCGGGACAGTGGGCCTTGTTGACCTGAGGGGGGCGAGG







GCGGTTGGCGCGCGCGCGCGTTGACGGAAACTAACGGACGCCTAA







CCGATCGGCGATTCTGTCGAGTTTACTTCGCGGGGAAGGCGGAAA







AGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene UBB. In some embodiments, the sequences from gene UBB comprise or consist of:











(SEQ ID NO: 1076)



GTTTGTGTGGTTTCTGGAAGCCTTTACTTTGGAATCCCAGTGTGA







GAAAGGTGCCCCTTCTTGTGTTTCAATGGGATTTTTATTTCGCGA







GTCTTGTGGGTTTGGTTTTGTTTTCAGTTTGCCTAACACCGTGCT







TAGGTTTGAGGCAGATTGGAGTTCGGTCGGGGGAGTTTGAATATC







CGGAACAGTTAGTGGGGAAAGCTGTGGACGCTTGGTAAGAGAGCG







CTCTGGATTTTCCGCTGTTGACGTTGAAACCTTGAATGACGAATT







TCGTATTAAGTGACTTAGCCTTGTAAAATTGAGGGGAGGCTTGCG







GAATATTAACGTATTTAAGGCATTTTGAAGGAATAGTTGCTAATT







TTGAAGAATATTAGGTGTAAAAGCAAGAAATACAATGATCCTGAG







GTGACACGCTTATGTTTTACTTTTAAACTAGGTCAAAATGCAGAT







CTTCGTGAAAACCCTTACCGGCAAGACCATCACCCTTGAGGTGGA







GCCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene LRRC75A-AS1. In some embodiments, the sequences from gene LRRC75A-AS1 comprise or consist of:











(SEQ ID NO: 1077)



CCTGAGGTCAGGAGTTCAAGACCAGCCTGGCTGGCCGACATGGTG







AAACCCCATCTCTACCAAAAATACAAAAATTAGCCGGGTGTGGTG







GCGTGTACTTGTAATCCCAGCTACTTTGGGGGCTGAGCCAGGAGA







ATCGCTTGAACCCGGGAGGCGGAGGTTGTAGTGAGTCGAAATCGT







GCCACTGCACTCCAGCCTGGGCAACAGCGAGACTCCATCTCAAAA







AAAAAAAAAAAATTACAACCTACTGAGAAGCTAATTAAGATTTGT







AGTCCTCAATAATTTACAAAAACTTAGAAAAATTACCTCTAATTA







GGTCTTAAGGGGCTTTTGTTAGGATGGACTTAAATTTGTAAAGTC







ATTAATTGACTGTCATCAGAAATGCCTGTCCTGCTCTGCATCTGC







AGTCTCTAAGGTGTATGCGCCTTCTCCATTCCCCCAGGTTTTGAG







AACAGAGTGCTGTTTTAGAGCTGGCAGCAGCATCTCAGCCCAAGA







GAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene LRRC75A-AS1. In some embodiments, the sequences from gene LRRC75A-AS1 comprise or consist of:











(SEQ ID NO: 1078)



AAAAAAAGATATCAAATGATGAAATCACCCAAAATAGCTGGAATT







ACCGGCAGATTGTGTAGTGGTGAACCTATGGTTTTCTGAAGATAT







CTCTTACCCTGTGATTGACTGTCAGTTGGTGCAGAAAGTGAGATT







GAAATGTAGTTTCTTTGCAGGTTATATTCCCAGAGGATGTCAGTC







CCAAGGACCAGTAGCTGCCATCAGTTTGGATTCTGAAAACTAACT







GGCATCAACACTGGGTGTAGAAACATGCTTGCCTTATGTATCAGA







GGACATGCTCAGCAGATCCAAGAGATATATTTGGCAACTTTTTCT







AGAAAAGGCACATTGGGTATCATTCATTACATTCTTGAGTTTTTT







TGGGTTTTTTTTTTTTTTTTTGAGACAGTCTTGCTGTATTGCCCA







GGCTGGAGTGTGGTGGCACAATCACAGCTCATTGCATCCTCAATC







ACCCAGGCCTAAGCAATCCTCCCACCTTGTAGCTGGGACTACAGC







TCAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SKAP1. In some embodiments, the sequences from gene SKAP1 comprise or consist of:











(SEQ ID NO: 1079)



GTAAGGACAGAAAGTGTGGGGCTTAAATAGAAAGTCAATTTTTTC







ATTCTTGATTTAGTCACCATAAACAGAATTATTCCATGTCATACA







CTTCCCATGGCTCCCTCACAATATAAATTCCCATTTATTCATAAT







TGTTAGGAAAAGTTTTAAATGAAAATCCAGATTCAGGAACCATTT







TCACATTTAAAAATACAAGGCTAGGCCGGGCGTGGTGGCTCACGC







CTGCAATCCTAGCACTTTGGGAGGCCGAGGCTGGTGAACTGCCTC







AGCTCAGGAGTTCAAGACCAGCCTGGGCAACATGCTGAAACCCAG







TCTCTACTAAACTACAGAAAATTAACCAGGTGTGGTAGCACATGC







CTGTAGTCCCAGCTACTTGGGAGGCTGAGGCATGAGAATTGCTTG







AACCCGGGAGGCGGAGCTTGCAGTGAGCTAAGATCACGCCACTGC







ACTCCAGCCTGGCGATGGAGCAAGACTCCATCTCAAAAATAAATA







AATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SKAP1. In some embodiments, the sequences from gene SKAP1 comprise or consist of:











(SEQ ID NO: 1080)



CAAAGTTCCTATGGACAAAGAAGGCCGAAAAGTGAGCTGTGATCC







AGAAGCCTGCTGGCTGACTAAGCCTTTCTTGTAAATAGGCAGAAG







TCACAGATTTACTCTGATCCTTCAAATGCATAATGAAGAGAAAAA







CTAGATAGTTAGTTGCCCTGGATCAGGAAAGTCTGGCGGGAAACC







TCTGCACTGCTTACTTACAAACCAAGTGAAAAATTACCTGCCTGA







TTTTCTTTTATTAATAAATAAATATATTTATCATATTGTTGAACG







GCATCCTACTAGGAGTGATCTGTAAATATTTAATGTTAGTTAATG







ATCTCCTTCTCCCATAGTCCTTTGCTCATGCTGAGTGTTTGGCTG







TATCTCATCTCAACTCCCCTTAATCTGAGGTTTCCTAGGGGCAAT







ATCAGAACCCTGGTTTCCATTTGCAGCAAAAACATGGGTACCTCG







GAAAAAATAGAGTCCTTTTAACTTTGATTTCTCCTCTCTTTGATC







AGTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene TOM1L1. In some embodiments, the sequences from gene TOM comprise or consist of:











(SEQ ID NO: 1081)



ATTCACCAACAGTTCAGTGATACTTGAATTCTGGTCTTCTTTCCC







AATCAGGCTGCTCCTATTATTCAGAGTCCTCAAATACTTGCTCTA







TGCATTCCGTCCACCTTTAAGTGACATTCAGTGGAGAGACAGAGT







GCAGTACGTTTGCTCCGCTTTATCCTGGACCGGAGACCTCACAGC







TTTCTTCTTAACCTGTGTTCTATACTACTTCTCATCCTTTAAGAA







ACGATGGGGTGGTAGCTACACTGATGGAAAGACTAAAGGCTCTGG







TCTTAGTGTTCCGTGCACACTCCACAGTCTCAGGGCTCCCACCAC







CCATGGCATCATAACCTGATGTGCACTAAAACCAATTGTTTTATA







AACAGTTATTACAAAATGACACTTTATGCAGTCTGAAGGTACCTT







TTAGGTTTTGTTTTTAGACTAGGAAAATTATCTTACTTTTTGCAT







TTGTGTTCTCACTGTAGGGTAATCATTCTGTGTTATCACACTATC







CTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene TOM1L1. In some embodiments, the sequences from gene TOM comprise or consist of:











(SEQ ID NO: 1082)



TAAGTAACAAAACTCTTTTCTAGCAGTGCATCTAGTCAGCAGATT







ATTGTAGCCAGAAAAGAACTCATGGTCATTAATTCCCATGTTCTG







CTCAGTATTGTGTTGATGCGCAGCCCCTGTTAGCAAGATGGTAAG







GATCAAAATTTTTTTGGAGTTTGTATTTGCTATCTCCACTTCCTT







TCCTTGTATTCTCTTTGACTTCAGACATGCTCTTCTACCACTCTA







TGGACATAACTCTTGTCAAGGTCACTGGTGACTTCTGTATTGTGA







AATTTAGAACTGCAGTTCCATAGTCCCTCATTTTATCAGCAACAA







ATGACAAAGTTGATCACTTCCTCCTTCTTGCACACATGGCTTTCA







GACACCAGTCACTTTGGTTCTTCTCCTGCCTCACGTTCATTTCTT







CTCCATCTCTTTTGGTGGCTCTTCCTCCTCTACTGACCTTTTCTT







GTTGGAGACCCCAGGGATCAGGCTTCAGACTTTTCTATCTACATT







CATT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ABCA6. In some embodiments, the sequences from gene ABCA6 comprise or consist of:











(SEQ ID NO: 1083)



GTAAGTTGATACATTTATTACCACTTTCTCTGCTTTAGATCTTAG







CTATATGTAGCTAATATTAATGCCCTAGTAGGTTACTGCAATCAC







TACTTTCATGTCCATTGTGTGTGTGCATGTGTGTGTCCTTGTGTG







TACTTGAAATAGTTTCTATGGAGTGAAAAAGTTATGTAATCCAAA







TAAAATTTGACTTGCCTTTTGCATAAAGGTCTGAACTAATGAAAT







GATGTATCAAAAAGAAAAAACAATGCTGACTCTAAAATATCAAAG







TCTTTTCTGTATAATTATTGAGGTTATTTTTGAGGGGTGGACCCA







ATTTTTTTTCCCTTAAGGGTGCTCATCACATGGTAGCTGATCACA







ATATCTCTTCTCCCTCTAATATTCTGTCTCATACTTACTTCTCGT







TTCACTCCGCTCTGCCTTCTCTGAACAAGAACCTCTAATTTCCAT







CTTCCAGGTTGTTTTCTGCAGAAAGTATGTAGATGCTCCTTCTTA







CATT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ABCA6. In some embodiments, the sequences from gene ABCA6 comprise or consist of:











(SEQ ID NO: 1084)



TCTGCCTTCTAGGTTCAAGCGATTTTTCTGCCTCAGCCTCCCGAG







TAGCTGGGACTACAGGCATGCACCACCATGCCTGGCTAATTTTTT







TGCATTTTTAGTAGAGACGGGTTTCACCATGTTGGTCGGGCTGGT







CTGGAACTCTTGACCTCAAATGATCTGCCCACCTCAGCCTCCCAA







AGTGCTGAAATTACAGGCATGAGCCACCGTGCCTGGCCTTGGCAT







CCTTTCTATTGGTTGGTGCCTATACCAGTGTTCAGCATCTATCAT







CTCTGTATGCATATATGGTAAAGGTAACGAAGATGAATGGCAATT







ATAAAGAAGGAGAATTGCGATTGAAAAAGGATATGCAATGAGCTT







CTGCTGTAGGATTAACAGTATAGTTATTAATCTAGAAGGTACAAG







GGTAGTCATTACATTGTTCTTTAGATGCTTTTGTATATATTAAAT







ATTTTGTAAATAAGTTAACATAATTTATATAACTTTTTGTCTTTA







TTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene AFMID. In some embodiments, the sequences from gene AFMID comprise or consist of:











(SEQ ID NO: 1085)



GCTTGGGAGGCTGAGGCAGGAGGATCGCTTGAGCCCAGGAGGTCG







AGGCTACAGTGATCCATGATCGCACCATTGCACTCCAGCCTGGGC







GACAGAGCGAGGCCCTATCGCTTAGAAAACAAAAAGTTACATGAA







GTTCCCCCAAGGTCCCGTAGCTAGCATGTGATGTGGCAGGGAGAG







AGCCTGTCCGCTTCACTCTCTCACATGGGACCAGAAAGGGGTGAT







GCTGTGCGTGGACAGGACATCTGGCAAGTGGTGTGTCCAGGACAC







AGGAAGCAGCGTAGTGGCCAAGCTGCCTCCAGCTGTCACATCTGT







GTGTCTCTGCAGTAAGGATGAGTCTGCCTTCATGGTCCACCCGCT







GACGGCACAGGGAGTGGCCGTGGTAATAGTGGCTTACGGCATCGC







CCCCAAAGGTAATAGGAGTGGTTGCTGCAGGTCCGAGGGCCGGTG







GGCTTTAGGAGGAAGTGCATCCCTGACCCAGCTCATGCTCTCTGT







GCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene AFMID. In some embodiments, the sequences from gene AFMID comprise or consist of:











(SEQ ID NO: 1086)



TGGGTGTTGCCAGTAGATTTTCTTCCTGTTGGACCTCAGTGGGTG







GGAGGACAGTGCAATCAGTACCTAGGATACAGCCAAGCTGCCCCT







CTGGCCTGTGGAGCAGAAAGCAAATTGGGACTCTTCGAAGGGGGC







CTGATGTTGTGGGGAAACTGCGTGCAAATCCAGCTCTCTGCTCTG







ACCCCTCCCAGGGGAATTTACCTGTGTGGACACTCAGCCGGGGCC







CACCTGGCTGCCATGATGCTCCTGGCCGACTGGACCAAGCATGGG







GTCACGCCCAACCTCAGAGGTTTCCATGGGAGCTACAGCCTGGCT







GGGCAACCTTCATCTCCCCATGAGCCTTGGGGTTTGGGCCAACTG







CTTGAAATCCTCCCGGCCATGAGTGGCTTGACCGCAGGGCTTCGA







GCCCTCTGAGCAAGGCCTTCTCTGCCTCCTCTGTGCCCCTTCCCC







TGGTCCTGCCCCTCTGGCGGTGGGGGTGGGCTGGCCCTGCCTTGC







TCCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene TTC39C. In some embodiments, the sequences from gene TTC39C comprise or consist of:









(SEQ ID NO: 1087)


AAGAACTGTTGATCCCAAAAGGGAAAAATTAGACATAGGGTGTGACGTG





TCTAGATACCTCCACCTGCGCTCATGCACACATTCCTGCGCCATAAAGG





ATTGATTTTTTTTTTTTAACCTTGGTGTTTTGTGTTTGAGCTGTGTGCT





AACAGTCAGTGTCAATGTCTGCCTTCTGAGCTGTGGGCGATTCCCATTG





TTGTGAGGTTTCCTCCAACTGGACGTTAAGTTACTGAGAACAGGAATCA





GGGATGCGATTGGTGCGTGTCTTCTGTGGCTACCTGGTCAGCATTCATA





AACGGTGATAATGGATTGAATGCTGCAGTTTCAACCTTCCTGACAACAG





AGGAGAGTGTTACTTTGGGGCATGAACAGTTATTGATCCAGCTGGGCCT





TGGTTAATGAGTCATTTGGCTTACAGTGTTCCCGAGTCACAGGCTGTCC





TGAAGATCACCTGCTGACTCATTGGTGCCCTTGTTTCCCCGAGGCCTCC





TGCAGTGAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene TTC39C. In some embodiments, the sequences from gene TTC39C comprise or consist of:









(SEQ ID NO: 1088)


TATGTGACATTTAGCATTTCTCAATGCCTAAGATTAAACTGATGCTTTG





AATCCTGATCATGCTTTTGAGCTGCTCCTCTTGGGTACTTGTGGCATCG





TGTTGGCAGACCCTGAGTGACATCTTCAGCGGCGGGAAAACGAAATGTA





TTTCACTGGGAGTAAGTAGAGCGGACATTTGAGAGTTTGCTGGGTCCCA





GACAGTGTTATGTGAATGAAAGTGTTGGAAATGTAATCTGGATCCTCAG





GGAATTTCTGAAGTGTCGGGAGAGGAGATTTGATGAGCAGAAGCAATTG





GAGAACAGCATTCTAGGGTATAGAAGCAAACTATCAACTGTATGGTGAA





GAGCTCACTGAGAACTGGGCTAATGGAGCGGCTTCGTGCATTCACTCAG





TAGCGATTGAGAGACTTCTGGGCCAGGGCTGGGCGCACACGGGGATGAA





ATATGGTCCCTTCCCTTTCCACACCTGTGTGGTAACAAGGGGCTAGATT





AGCAAACAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MOCOS. In some embodiments, the sequences from gene MOCOS comprise or consist of:









(SEQ ID NO: 1089)


GTTCAGTACTGAAGACCTAAACATTTGTCACAAGGGCAGTACCATCCAC





TATTTACACACATTTAAAATTATTAATGTGAGGTCCAGGTTTGCCAGTT





ATAACAAACTTGTTTTCTTAAAAGTGAGTTAGTGGTGCCAGGCGTGGTG





GCTCATGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGATTA





CTTGAGCCCAGGAGCTCAAGACAAGCCTGGGCAACATGGTGAAACTCCA





TCTCTGCAAAAAATACAAAAATTAGTCAGGCATGGTGGTGAATGCCTGT





CGTCCCAGCTACTTGGGAGGCTGAGGTGAGAGGATCACCTGAGCCCAGG





AGGTTGAGGCTGCAGTGAGCTGTGATCATACCACTGCACTCCAGCCTGG





GTGACAGAGTGAGTGAGACCGTGTCTCAAAAAAAAAAAAAAAAAAAAGA





GTGAATTGAGAATTTACATAAAGCTCATGATTCTCTTGTGTTTCATGAT





TGATTTGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene MOCOS. In some embodiments, the sequences from gene MOCOS comprise or consist of:









(SEQ ID NO: 1090)


TGGGTTTTCTTTCCATCCTGCTGACTTTATTACAACTCATCCTAGTTCC





AGACGAGAGCAATGTAGTGTGACAAAGTCCATGCACTGTTGAAGAGGCC





ACCAGGCCCTCAGCCACATGCCTTTCCACCTTCATTCACCCTCGTTTTT





CAGTCCTCTTTACTCAGCCAAAAGTGGTAACTTTCAAATGGGAAGAAAG





AGGCCAGTTCTTCTGATGGCAGGTGGGAAACTGTTGGGTCTTTGCCATA





GTAGAATAAGCTGTTCACTTCACTTCCTGGTCCACTAAAATGTCCAGAG





TTGCACTGAGCACTCAGGCATGGTGGTAGGTGGTTATCAGACGGGGTGT





CACAGATGGACAACAGGAACCAAATGTTACTCCCAGAGCAGCACTGTGG





GGAATGGATTCAATCGTTGGGTGTTTGGAAGACGGTGCTGGTTTTTCCT





TGAAAGCACATCTGCATCCCCATTTTTATTTATTTTTTTGAGGTGGGGT





CTCGCTCTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PET100. In some embodiments, the sequences from gene PET100 comprise or consist of:









(SEQ ID NO: 1091)


CTAATGTCTGCCTCCCATAAGCCGACCCTGCCCTGATGTGGGGCTCTGG





AGGCCTTTGGCTCGTGTCCCATTTGTGACTTTTCCTTACAGAGGGAGCT





GTGGCCACCTGAGAAGGTAAGTGATCTCTTCTTCCTGCCAGAGGGATGG





AGGAGGCTGGATTCTTGTATCCGGGATGGGTTTTAGATGAAAATTTTAG





GCCAGGCGCAGTGGCTCATGCTTGTAATCCCAGGGCATGGGAGGCTGAG





GTGGGTAGATCACTTGAGGTCAGGAGTTCAAAACCAGCCTGGCCAACAT





GGCGAAACCCCATGTCTACAGAAATACAAAACTTAGCCAGGCATGGTGG





CAGGCGCCTATAATTCCAGCTGCTTGGGAGGCTGAGGGAGAAGAATCAC





TTGAACCCAGGAGGTGGAGGTTGCAGTGAGCCCAAGATCACGCCACTGC





ACTCCAGCCTGGGCAATAGAGTGAGACTTTGTCTCAAAAAGAAACATTT





TACTCTCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PET100. In some embodiments, the sequences from gene PET100 comprise or consist of:









(SEQ ID NO: 1092)


TAATTGGCAGGGGGCTTCCAGTGAAGACACTGAACCCTGAAGCCGTGGT





CTGGGGCAGGGTGGTGGGAGAGGTGGGCGGGGGGGGGTGGTCCCGGCTC





TGAGGTGTGTGCCCCTCCCCCCTTCCTGTCCCACCCGCTTCTCCACCCC





TAGCTTCAAGAGATAGAGGAATTCAAAGAGAGGTTACGGAAGCGGCGGG





AGGAGAAGCTCCTTCGCGACGCCCAGCAGAACTCCTGAGGCCTCCAAGT





GGGAGTCCTAGCCCCTCCCCTGATGAAATATACATATACTCAGTTCCTT





GTTATTCATTTAAGTGTTTTATTCTTTTATCAGTTTTTGGGGGCCGAGT





GAGACCCAGGATGCCTCAGGCCCTCAGGGGGCTTGTGTCGGGGGCTGGG





GGCTGCTGTTCCGACGGAAGCCGAGAGCGGTCGGGTCCTGAGGGGTGAG





CAGGGGTTGGGGACTGAGGGTCCCAGCTCGTTTCTGTGCTCCGTCCTGT





GGATGAAGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PRKACA. In some embodiments, the sequences from gene PRKACA comprise or consist of:









(SEQ ID NO: 1093)


GTGAGGCTGAGTCCTGTTACTATAGCAACTTCCTAGGCACACTCTGCCC





CTTAAGTCAGGGAGGGGCTGCAGCGGGGTCTGGGGCACAGGCGAAGAGA





AGAAGGATGTGAACCCCTGGCTGGCCCTGGAAACGCCTCCTGTACAGCC





CCGGGCTGTGCGGCCTGGGGAAGAGAGAGGGGATCGGTGGCCCAGTTCA





GACAAGGGAGGATGGTCCACCCGGCAGGGGGCCAGCTCAGAGAGGGGTG





GTGGAGTGGTCCATCCCAGTGTTGGGGTTCAGCTCAGACTGTAGGATGA





GCCATCCCAGTGTTGGGGTCCAGCTCAGAATGGGGGTCGTTCCAGCCAG





TGTTGGGGTCCAGCTCAGAGTTGTGTCATTTAGGTAAGCCCAGAGCAGG





GAATCCAGGCTCCACTGGAGGAATCAGCCAGCCTCGAGTAGGGGGCCCA





GCCCAGGCTGGGCATTCCTGCCCCAGAATTCCCAGGACAGCCAGATTTC





AAAAGGGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PRKACA. In some embodiments, the sequences from gene PRKACA comprise or consist of:









(SEQ ID NO: 1094)


CAGCGGCCCCAGGGGGCAGGCGCCCAGGTCAGACAGCCAAGTGGGGCTC





AGGTCGTGGCTGGCACTGGCTGAAGATACCCCTGGGAGCTGGCCCACAG





GGGAAGGGGCAGAGCTAGTAAAAGGGAGCCCCCGATCCCCAGGACACAA





GCAGATCCTGGCAAAGAGAGAAAAGCACCCCCCAAGAAGACCCTAGATG





GGAAGAACTAGTGCCGTGTAACATCCTATGGATGCCTAAGACGGCCAAA





CACCTTCTGTGCCTTGGCAGTTGTATGGGTGCCCAAGAGGTCTTATCAA





GGCAGCTCTCCCCTTAGCGCAACCGCGTCTACAGAAACTCGGAGGGCCC





TATCTGTCCCTCCTCCACACCCAGTGGCCTCTGGGTTGGGTTTCTCTTC





CTGCTCCCACCCCACGGCTCCCTAGCTCCCCCTGCAGGCAGGGTTCTGG





GGACAGACAGCCGAACAGACACGGCAGGTCTCATGAGCCTTCCCAGCCA





CCGTAGTGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SLC27A5. In some embodiments, the sequences from gene SLC27A5 comprise or consist of:









(SEQ ID NO: 1095)


GTCCGAGGCAGGGTTTCTCGGGGCGGGGCTGGCGCGGGGCCACGGACGC





TTTCAGATCCTAGAGGGAGTCAGGCTGAAGAGGCGGGACTTTCCTGTCG





TAAGAGGGCGGAACTTGGAGTCAGGACTTCTCACGCGAAGGCGGGGCTT





CTCGATTTTCGAGGGGGCGGGGCTGACTGGGCCTCCTGATCCCCAAAAG





GGGCGGGGCGGGGGCAAGAGGCGGGGCCTCTCCTTCCCGGGGGCGTGGT





CACAAGCGCCGGCTCCCTCCTTGCAGATGGAAGGGCGAGAACGTGTCCA





CGCACGAGGTGGAGGGCGTGTTGTCGCAGGTGGACTTCTTGCAACAGGT





TAACGTGTATGGCGTGTGCGTGCCAGGTGCGGAGGGTTCTCATTTTAAA





CTTCCAACAGCTTTGTGGGTGGAGGGGTCGTTATTCTGATTTTACAGGT





GGGGAAGTGCTAGGGCCCAGAGAGGTTTAGAGACTTGCTGGCTCTCACA





GCCAGTGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene SLC27A5. In some embodiments, the sequences from gene SLC27A5 comprise or consist of:









(SEQ ID NO: 1096)


TTACCACCATCTGGGGCCTCCGCCTCAGCTTCTCGGCTCTGTCCAGCTC





CCCTGCCTTGATCTCTTGCTCTGATTTACTGGGTCTCTGGGTGTCCATC





TGATTTGTTCTCTTGAGCTTTCTGTGGAAGACGGCATTCCAGCCATTGG





TCCCTTGTCCTGTACCTTCTGTTAGTGTCTTCTCCCAGGGCCTTGCAAG





GTCCTGGTCATATCTATTCTCACTGCCATTTGTCTGTCCGTCTTCCATC





CCTTGTTATCCTTTTCCTGTTTCTCATGGGCTCCCCATACCGCCTTCTC





TATACTTCTCTCTCATTTTCTGTGTCGCTTGTCGCAGTCTCTCAAGGTC





TCTGAATGTCTCTCTCATCTCTGGATCTCCCTGTCTGTGTCTTTGGGTC





TGTCCTTGGCAGGGTGCTGGGTGATGAAGAGTCCACCGTTGTTGAAAAC





CTCCAGCAGGGGGCTTGGCTGGTGGGCTCCAGGGGTTCTTGTTCCTGCT





CCTACCCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ACSS2. In some embodiments, the sequences from gene ACSS2 comprise or consist of:









(SEQ ID NO: 1097)


CACTTGATGAGGTCTGCACCTTCTCAGTGATGTCCACTCCCACAGTGTA





GTTGGCAGCTCCTCACTGGAGTTTGAGTCCTCTGGTAGGGTCCACGTCT





CCTTAGTAAAGTCTATATCCTCTGTATCTCTTAGGGGAAATCCATGTCT





CCTCACTAGGGTAAGTGCCCTTCAAAGCCATTCATGTCCTATTAGCAGG





GCCCAGGGCCCTTCAGTCAGATCTGAGAACCCCCAGCAACATCTGTGCT





CCCTTAGTGGAGTCCATGTACTGTCAGTGAAGTGTGTGTCCCTTCGGTG





CAGTGAATATCTCCTTTATGAAAGTATCTGACCCCTTGGTGGGGTCTAT





GTCCACCCCAAGAGGTCTGTACTCCTTCAGTGGGCCCTCAGATTCCCGC





CCTCCTTGGGATTGGGCAGACTCTCGGTCTCCCATAGAGAGATTTTTTT





TCAGCTTAGGGTGTGCAAGGTTTACAGTCAGCTGGGCAAAGTGCAGGGA





AGTAGCTGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene ACSS2. In some embodiments, the sequences from gene ACSS2 comprise or consist of:









(SEQ ID NO: 1098)


TGAGAGATGGAGGAGGGTCCTGCCTGACTTCTTTCTGGGGTGGAGGGGC





TAGAGAGAAGCTAGAGGTAATTAAGCCCAGGTTGTGGTGGTGGTGGTGG





GGGTTTCCCTTGGTGGAGTAAAGAGATTGTGAAGGAAAGAAGAGAATGT





GGGCCAGGTGTGGTGGCTCACGCCTGTAATCCTAGCACTTTGGGAGCCC





AAGGCGGGCAGATCACCTGAGGTCAGGAGTTCAAGACCAGCCTGGACAA





CATGGTGAAATCCCATCTCTACTAAAAATACAAAAATTAGCTGGGTGTG





GTGGTGTGCACCTGTAATTCCAGCTACTCGGGAGGCTGAGGCAGGAGAA





TTGCTTGAATCCAGGAGGCAGAGGTTGCAGTCAGCCGAGATTGTGCCAT





TGCACTCCAGCCTGGGCGACAGAGCGAGAGACTCCATCTCAAAACAAAA





ACAACAACAAAAAAAGAGAATGTGAATGATTTTTCCTATTTGCACAGAC





TTTGATATT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene LBP. In some embodiments, the sequences from gene LBP comprise or consist of:









(SEQ ID NO: 1099)


CCAAGGCAGAAAATCGCTTGAGCTCAGGAGTTCAAGACCAGCCTGGGCA





ACGTGGTAAAACCTGTCTCCACAAAAAAATACAAAAATTAGCCAGGCAT





GATGGCACATGCCTGTAGTCTCAGCTACTTCAGGGGCTGAGTTGGGAGG





ATGGCTTCAGGCCAGGCGACAGAGGTTGCAGTGAGCTGAGATTGTGCCA





CTGCACTCCAGCCTGGGTGACAAGGTGAGATCCTGTCCCCCACCCCCCC





GCAAAAGAAAAGAAAAATGTGGATTCTCAAGCCGCTTCCCCAAATCTGC





TGAATGTGATCATGCGATTGGGTGCCGCAGGCACTGGGAAGCCAGACAC





ACCGAATCAGGCTGGATGTGCTGGGACACAGCAGGGCGCCGGGAAGGGG





TGGAGAGGGCTCCCTTTGTGGACTGGCCTGACCAGGGACCAGGGCCTGG





CTTTCCCAGGCACCCGGCCATCCCCAAGTTCAGTGGCAGACTGTGCTTC





TCTCCCCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene LBP. In some embodiments, the sequences from gene LBP comprise or consist of:









(SEQ ID NO: 1100)


TAAGAAGGTCCAAGCCTCTGGCCTCCCCCGAGCTTGGCGAGGGCTGAAT





GGAAGACCTCACTGACCAATGGCCCTGGACCAGGCCATGGGGGCTGGTT





TAGACGTACTTGGGCCCAAAGCCCAGGGAGGACAAATCATTTGCCCAGG





TCATGGAGCTGTTGTGGGGCACGCTGGGGACAAAGCTCCAAGTCCGTTC





GGTTGGGCAGAAAATGGTCAACTGCTTGACCAAGGGTTAAACAGCAGGG





GCTAAACAGAGACTCCAGCCCCTCCCTCTGCTCCACCTTGCTCAGTGCC





CCACACTGCCCTGCATTCCACCAAGGTCCGAAAGGAAACCATTCCTCAG





GCGCCTGCAAGAGACCAGGCAGGAAAGCTCAGCGAGGGCGTGGGCCAGG





TGGGAACTGGGCGAGCTCATGAGCACCAGCCCTGGATGGGGAGGCAGCA





ACACTGTCAGACTGGCCTGTTAAAAAGGAGTATGTGAAATCTTCAGAGT





GTATCATCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PABPC1L. In some embodiments, the sequences from gene PABPC1L comprise or consist of:









(SEQ ID NO: 1101)


AATCCCTCCATGCCAAGGTAAGCAGGAGCCTGGGCAGCAGGGAGCCCGA





GGGGGCAGGAGGAGAGCTAACGACAAGGGCTCTCCTAGTGGAGCCAGTG





GATAGCTGTTTGGCCAGATAAAGTGTTTCTTCTTGCTAATACCTGAAGG





CCTGCTGAATCTTAAGAGTGGAAGGGGCCTTAGGTAAGGGTTTGCTACT





TCAGCCCTTCCGTAGACTAATTCTGTGTTGTTCTGTAGGTAGGTCTTTT





GGTACAAGGAAGTGTGTTGTGACTCAGTGTAAGGGTTAAGATTTTTCCA





GCTGTCCAGCAATGTACCTCTGTGTCAAAGGTGACAGACAGGGCCATGG





GATCTTGCAAGCAGTGGTGGATGGGAGGGTTCCACAGAAACCCTGTGGA





TGGAGGAGGATCCTGTATCCAGGATGGTGAGCGAGGGGGAGATCAAGGC





TCTTCTGTGACCTGCTAAGATAGCTGCACTAAGCCCCCCCGCCACTCAT





GTCTCACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene PABPC1L. In some embodiments, the sequences from gene PABPC1L comprise or consist of:











(SEQ ID NO: 1102)



TGGGTGGAATGGTGACAGAAGCAGCTGAGCCCGGGAGAAGCTGTA







AGGAAATAGGCAGGGACTGACTTTCACAAACACTGAGAATTGCGC







CGTGTTCCTGGCTTTCTCTGAAGCACCAAAGAGTTTGGAGGAAGT







TGTGAGAATCAGAGGAGATGATTAACTGTGATCGGAATGGGGGGT







GGTGGAGGGTGAAGGAGGTGCCCTCTTGCCAGCTGCCCTGCCTGG







CTTCATGCAGAATAGTTTGGGCTTTCCCAGGGCCCTGCCTGGCTC







CATTCTGCCTGCCTGAAGGCCTGGCCAGGATGTAACCACCTTCTT







TATTGAAGACTGGGTGTCTGCCATACTTTAAAAACACTTATTTTT







ACCTTTTTGTCCTCAGAAAAGGAAATCCTCGCTTCCATGGCTGCC







AAAAGGACAGTGTTTCTGGCTCTCAGCCCTAAGGCCCTGCAAACT







CTAACTTATTTCCCAATTAGTCTGTATCTATACTTGGGCTCTGTA







TGTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene UBE2V1. In some embodiments, the sequences from gene UBE2V1 comprise or consist of:











(SEQ ID NO: 1103)



GTATGCTTGCAGGTTTTGCTGTTTCCTTTCAAATACCATATGTAG







TACTATTTAATATTTTTCTTGTAATCAACTGACTCTTTTGCTTAC







ATTTATTTTAAAGGAAACTTTTACCATTATTATAAATGGGAAGTT







AGTATATTACTTACCCTAAATAAGTTATTCTTAAACATAAATACG







TGACTGTAAAACGAAGTGTTCAATCACATACCACCGAAAGTCCTC







TAACATACCACTACCAACTCTGTCCTTCAGTTGCTTGGACCAAAA







ACCTGGGAGCTGTAGTTGACTCTTGTCTCTCACACCTCACAGGCA







ACCTGATAGCAAATCTCCACAGCTCAGACCTCTGAAATGCCTAGA







ATTACGCCACTCCTCACTACTTTCCTGCTATCACCCTGGTCCAAA







CGGGTATTATCTTTTGCTAGGATTATTGCAGTAGCCTCCTAGCTG







CTTCTGCCCTTGCTCCCTTCACTCTCATCCTCACACAGCAGTCTG







AGGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the heart comprises or consists of sequences from the gene UBE2V1. In some embodiments, the sequences from gene UBE2V1 comprise or consist of:











(SEQ ID NO: 1104)



AACAGCTGTTAACATCTTGTTCTGTATCCTTACCAACTCTATAAA







GCTCCTTAAGATGTCACTTATCATCTATATGAGTTTGCTAGTGCT







GTCATAACAAAATACCATAGACTGGGTGGGGCTTAAACAACAAAT







TTCTCACAGCTCTAGAGACCAGAAGCCCAAGATCAAGGTGTCAGC







AGGTTTTGTCACATTCTGAGGTACTGGGAGTTAGGACTTAAGTAT







ATAAATTTGAGGGGAACACAGTTCAGCCCAGCCCATAGCACGATC







TGTGAACAAGTACCTCAGTCATCAGCCTTCCTATCAATATGATTC







TTTTGATGTTCATTTTTATTAGTGCAGGCTTGTAAATTAACCAGT







AGTAATGTAGTGACTTTTCCCCCGCTAGCCTAATTAGGATTATTA







AAGATTTAGATTGAACCCTTTCTGAACAATAGTTGTGTTGTCTGG







CTTTTTGCCTATAATGTCACCACTATCTTTCAGTTTTATGTTTGC







TACA







Intronic Domains that Promote Trans-Splicing Primarily in Muscle Tissue


In some embodiments of the compositions of the disclosure, there may be an intronic domain that promotes trans-splicing primarily in muscle tissue.


In some embodiments of the compositions of the disclosure, the intronic domain is derived or isolated from human genes that feature exons that are primarily included in muscle tissue during the process of RNA splicing.


In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene CDK11B. In some embodiments, the sequences from gene CDK11B comprise or consist of:











(SEQ ID NO: 1105)



GTAAGAACCTCCCTGGAGACAGAGCCCCCTCCTTGTCTGTCTCCG







AGTGCAGCAGCCCTCTCCTAAGACAGCCGGTCCTGCGCAGCGTGG







GGCACGCCAGGGTCTGGGAGGCCGCCTGATTCGTTGGCCGCTCCA







GGCTGTGCCGGTGGGGCCCGCATTCCTCAGCGAGGCACAGTGCTG







ACCAAGGGCGTCCTGAGTGCCCAGCAGGGCCGAGGAGCTGGGAAG







CCCAGGACCAGTTCCCCACAGGGGAGGCCCGTCAGATGGTGGCTC







CGGGCCTGTCCCTGCAGAGCAGAGGCCTGGCCCAGCCTCCGACAG







GCAGTGAGGTTATGTCCTCAGCCTGGTGGACGTGGATGTGGGCTT







GTCCCCCACATGACACCCGACGCCTCCTCCCAGCTCAGCATCCGG







GCTCTGTCTCAGCCGAGCCTGCCAGCACTCTTAGGCCTCGGTGCC







CTGTGTCCAGGGGACGAGAGCCCAGCGCCAGCACCTCCCAGCGGG







ACAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene CDK11B. In some embodiments, the sequences from gene CDK11B comprise or consist of:











(SEQ ID NO: 1106)



TTAGAAACCGCAGAGAAGTAGCTGGCGGGCGGGGGAGTTGAAACT







CCCTTCTATCTAGGACTCTCTCGGGACACCTCCACATCGATTCTT







TTTGTTTGTTTTTTTGAGATGGAATCTTGATCTGTTGCCCAGGCT







GGAGTGCAGTGGCATGATCTCGGCTCACTGCAACCTCTGCCTCCC







GGGTTCAAGTGATCCTCCCGCCTCAGCCTCCCAAGTAGCTGGGAG







TACAGGTGTGCGCCAGCACGCCCGGATAATTTTTTTATTTTTAGT







CGAGACGGGGTTTCACCACGTTGGCCAGGATGGTCTCGAACTCCT







GACCTCAGGTGATCCACCCGCCTCGGCCTCCCAAAGCGCTGGGAT







TACAGGCGTGAGCCACCGCGCCCGGCCTGCATATCCATTCTTGGG







AACACTTGTTGCTTAGCTGAAGCGGAGCCCGCATCCTGCCTGTGG







CAGCACTCGCCCCGGTGCTGGTCTGAGCAGACGCCTCCTTTCTCT







TGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene HP1BP3. In some embodiments, the sequences from gene HP1BP3 comprise or consist of:











(SEQ ID NO: 1107)



GTGAGTGTGCGGAGCGCCGAGGCTCGCGTTCCGCCGCTGCTTCCG







TCGCCGGGGCGGGCGCCGCGGTGTCCCTGCCGAGCGTGAGCCCCG







AACTGTCACCGCGCCGAGCCCCAGCCTCATCCGTCGGTGTCCGCG







GTCCGAGCTTTCCCCGCCTCGCTGGCGGCCCGCGCTCTCCCCCGC







CGTGCGCGTTCGCCGCTGTGGCCTGTAGCTACCTCGCCTCGCTGC







TTTGTCTCCGCTGCTCTTGCCGTCCTTCCCGCGGCCTTCTGCGGG







CGGGGCTGTCGGCTGTCGCTTTGCTGCTCCGCGACGGCGGCCTTG







AGTCTCGCTCCCCCGGTGGGAAACGTAGAACTGGTTACTCGCCAC







CTTAGGCGGTGGGGTTTGAATGAGAGCGGTGCCTCGGTAGGTCGT







CAGAAAGTTACAGTCGCCCATCTGAGCGTTTGTAAGAGATGGAGG







CAGGAGTGGTGAGGTGAGAGCCCCAGATCTGAGGGTTGGTACTGC







ATTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene HP1BP3. In some embodiments, the sequences from gene HP1BP3 comprise or consist of:











(SEQ ID NO: 1108)



CAGGACCGAACAGGCGGCCGGGGAGTGGGCGCGGGCTCGCTGGGC







CCCGAAGGCGAGTGGCGGAGGCCGCTCGCCCGGGGCGCCGACGCC







GCTCCCAGAGAGGAGGGGGCGGGGCGCCCGGGTCCCCGGAGGTGG







CGCCTTTTGTCTGCCTTACCCGGCCGCTCCGCCCCGCTCCCTTCC







TCTCTCCTGCGACCTAGCCCCCTGCCCTTTTGGTGGACTCCTCCT







CAGGGGCCCTCCGGCGCGCGCGCTCCCGCCCTTGTTTTGTTTGGA







TTTGGGGGAGGAGGGGGAGCTCTTCTCTGGCCGTGCGACCCTGCG







TCCCAGCGCGCCTGCGTCGGCGTCCTCTGCCCGCCCCGATTGGCT







GAGGGCTGCGCATGCTCCGAGGCCCGCCGCGGTCCGGCGTCCGCC







CGCGCCCTCCCCTCACCCCACTCCCTCAGCGGTTTTCGGAGCCGG







TTCGAAAGATGCCGGCGGCGGTGACTGGCTGCGGCGGGCCCGCCC







CCCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene FNDC5. In some embodiments, the sequences from gene FNDC5 comprise or consist of:











(SEQ ID NO: 1109)



TCCCTCAGCCCCAGTGAACGTCACCGTCAGGCACCTCAAGGCCAAC







TCTGCAGTGGTGAGCTGGGATGTTCTGGAGGATGAGGTTGTCATC







GGATTTGCCATCTCCCAGCAGGTAATGCTCCACTGTCCTTAGGAG







GGAAGGACACATCAAGACAGTAGTGGCAGATGGGGGTAGGAGTGT







GGCAGTGGTGGTGATGGAGAAATAGAAAGGAAATAGGAACTTGGG







GCAAGGGAGATGAACAGCAATTTGGACATATGTCTGAAAATTGGG







GCCCCATTCAGACATATGTCTGAAAATTCTCTAGAAGAGAATCAG







TCAGTGAGGGAGGGTTTGGGAACTTTCAAGTGGAACACAGAAATT







AAGGTTTTGATCTCAGAGACATCTGCATTTAGGACCTAGCTCCAG







TGCTTACTAGATGTGTGGCCTTGGGCAACTTCCCCAACCTCTCTG







AACCTGTTTCCTTCCCTGTAAAATGGAGATATTGATAGCGGAGGT







GAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene FNDC5. In some embodiments, the sequences from gene FNDC5 comprise or consist of:











(SEQ ID NO: 1110)



TCCCAGTCCAGGGCTCACCCCAGCCTGACACTTCTCGATCTCTGG







GTCTTTCTCCTTCGCGCAAGGTCACTCATTGGACAGGGCAGTATT







GTCAAAAGAGCACTGGACTGGGGAGTCCTTTTGTCTGCTTTTCAT







CAGCTAACCTTCAGTTTCTGTCCACCTATGTCCCCTACATAACTC







TCTCCACAACTCTAACCACCCATCCCTTAGATAGGGCCCCGGCCC







AGGCCCATCACTTTGAGCCTTGGACCCCTCATGGGGCCGGCCTGT







GGCCAGCAGGGGCTTCCGTCCCAGTCATGAGGAGCCTGGGGCCTC







TTGGGCTTGGTTCTTCAACCCACTGAAGAGCTGAGAGCCATGTCA







CTGGACCCCACTTGAGGCCTTAGTTTCTTCCTTCCCCTCCTGCAT







GGCCTTCCCCAGGTTTCCCCTTCTTACTTATGAGACAGTGGGTCT







GGGCCTCACAATAGGGGATGCTCAGGCTTAGGTGCCTCCCCGTCC







CCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene RAP1A. In some embodiments, the sequences from gene RAP1A comprise or consist of:











(SEQ ID NO: 1111)



TGCGCGGCTCCTTTAAGCGGACTCCGGAACGCCGTGGGGAAGGCG







GGGCCGAAGCCCAGCCATCGCCAACTTGGAGGGGCGGGGCAGAAC







CAGGGGGCGGGGCCTGCGTGGTGCGTGCGTGCGCGTTCTGGAGGA







GGCGCCGCCGCCGCTCCCGAGGCCCCTGCCGCCGCCGCTCCCGCT







GCTGTCGCCGCGCAGAGCCGGAGCAGGAGCCACGGCCGAGAGGAG







GGAGGAGGAGGAGGAGGAGGTGGAGGAGGTGGAGGAGGTGGAGGA







GGCGCCGGACCGGGGGGGTGAGTAAGGGGCGGGGAGCTCCAGACG







GCCCCAGAGGGAGCGGGGGGCGCGGGTCGGCCGAGCCGAGGGTGA







GGCGGCTCATGTCGCCGGTCAGTCGCCTGGCTCCCCCTTCCTCCT







CCGCGTTCCATCGGTGTCGGGGCGGCGGCCCGGGGGCCTGGCCGG







CGGGAGGGGCCGGAGATCTGGGAGGCCCGGCCGCCGCCAGGGCCA







GAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene RAP1A. In some embodiments, the sequences from gene RAP1A comprise or consist of:











(SEQ ID NO: 1112)



TTGGACATCGCTGCCTTCCTCCTCTTGCATGTCCCCTGTCTAGGG







TCAGAGCTTCCTTCAAACCCAGCTCTCCCTTTTGGGCCCGCGGGG







GCGGGGGGGAGGGACACACGGGGAACGGGGTTGGAGGGTTGGGTG







AATTCTCTGTCCTCTGCTGTAGGAGTGCGGGTAGACTGGAGCATT







GCTGTCTCCGGGCTCACTTCTGTCAGGGTTCAGGGTCTCCCTTTC







CTTCCTTTATTCCCCCAGTTAATAGGGCTGACATCTACATCCCAC







TCCACCTTCCAGCCCCTTTCCCGCCTTCTTTACCCTCATGTCTTC







CGTAGCTTACCATGTGTTCGCCTTTTCAGTGTTACTTTTCATGGG







TCTCTGCTTCTTCCCTGAATTTTGTTAGGGAAGAGGTCACGGTGT







GATTCTTGTTATCCTTCAAACTAGCGGCTTGGTAAGAGTATCTCT







TGCCCCCTTAATCAGGAAGTTAAGGACGGTGTCTTCAGCCGAGTT







GTGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene TMOD4. In some embodiments, the sequences from gene TMOD4 comprise or consist of:











(SEQ ID NO: 1113)



GTGAGTGTGTTTGGGGAGCATGGTCCCCAGGGCTCTGGGCAGGCA







GTCCTAGTGTCTGGGAGAAGGTAGAAAAGGGTGAAATTGATGATT







CTGGGAGAGGACATGCTGCATGAGTGATAAGAAAACCTGTGGAGT







GCCAGGTGAGGTGGCTCATGCCTGTAATCCCAGCATTTAGGGAGG







CCGAGGCAGGTGGATCACCTGAGGTCAGGAGTTCAAGACCAGCCT







GGCCAACATAGTGAAACCTTGTCTCTACTAAAACTACAAAAATTA







GCTGGTTGCATGCCTGTAATCCCAGATATTATACTTGGGAAGCAG







AGGCACAAGAATTTCTTGAACCCGGGAGGTCAAGGCTGCTGTGAG







TTGAGATTGTGCCACTGCATTCCAGCCTAGGTGGATAGAGCGAGA







CTCCATCTCAAAAAAAAAAAAAAAGAAAAGAAAAGAAAACCAATG







GGGAGGAGATTATGGGGAACATGTTGGGGTTCCCTTCCTGCCCTC







ATTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene TMOD4. In some embodiments, the sequences from gene TMOD4 comprise or consist of:











(SEQ ID NO: 1114)



AGAGCGAGACTCCGTCTCAAAAAACAAAAAACAAAAACAAAAACA







ACAACAAAAAAAATTAGCCAGGCATGGCAGCATGCACCTGTAATC







CCAGTTACTTGGGAGGCTGAGGCAGAAGAATTGCTTGAACCCGGG







AGGTGGAGGTGGCAGTGAGCCAAGATCGCACCACTGCACTCCAGC







CTGGGGGACAGAGTGAGACTCCGTCTCGGGGGGAAAAAAAGTGGA







ACCATTCTGGCTGAAAGAGGAGAGTCTCTACAGACTCCTTTATCC







TCTAGTGGCTCATAAGGGACCTTCCCTTAGCCCTTTCTCAATGTT







TTAGAAACTACTGCCTTAAGAAGTTTATGGAAAGGACCCCTGGGG







CTGAAAGAGGGTGGGAAACTTCTTTGGCAAAGTGTAAGTGGTCAG







GCTTTACAAATGCAACAGATTCAGAAATAGGTAGGAGGGAGGCTT







GGAACAGGTTCCTTCTCATTCCCTGTGACTCCTCTTCTTACCCTC







TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene DAP3. In some embodiments, the sequences from gene DAP3 comprise or consist of:











(SEQ ID NO: 1115)



GTTTTTGTTTTTTTTTTTTTTTTTTTGAGACAGAGTCTCACTCTG







TCACCTGGGCTAGCGTACAGTGGTGTGATATCAGCTCACTGCAAC







CTCTGGATGCAAGCAATCCTCCTGCCTTAGCCTCCAGAGTAGCTG







GGACTACAGGTGCATATCACCACTCCTGACTAATTTTTTTTTTTT







TTTTTTTTTGAGACACAGTCTTGCTCTGTCGCCCAGGCTGGAGTG







CAGTGGCATGATCTCAGCTCACTGTAAGCTCCGCCTCCCAGTTTC







ATGCCATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGG







CGCCCACCACCACACCTAGCTATTTTTTTGCATTTTTAGTAGAGA







CGGGTTTTCACCGTGTTAGCCAGGACCGTCTCGATCTGACCTCGT







GATCTGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTG







AGCCACTGCGCGTGGCCTCCGACTAATTTTTTAAAATATTTTTTG







GTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene DAP3. In some embodiments, the sequences from gene DAP3 comprise or consist of:











(SEQ ID NO: 1116)



TATGAGCCATTACACCTGGCCTATTTTCTTTTTTTTTTTTGAGGT







GGTCTCACTCTGTCATCCATGCTGGAATGTAGTGCATCTCAGCTC







ACTGTGACCTCCACCTCCTCCCAAGCTCAAGTGATCCTCCTGCCT







CGGCCTTCCGAGGAGCTGGGACTACAGGCATGCACTACCATGCCC







TGCTAATTTTTATATTTTTTGTAAAGACAGGGTCTCACTTTGTTG







CTGAGGCCAGTCTTGAACTCCTGGGCTCAAGCAATCCACCCACCT







CAGCCCCGAGAAGTGTTCCTCCCAAAGTGTGGGGATTGTACATGT







GAGCCACTGTGTCCCGCCTGACTTTTTTATTTTCCCAGTATGATG







ATTGTGAATGGTATCTGGTTTTCATCATTTTAATTAGCATTTCCC







TGATTTGTAATGAGGTTCAAAAATCTTTTCATGGGTTCACTAGCC







TTTTGTGTTTCGTTTTCTGTGAAATGCCTGTTCATGCCTTTTGCT







CATT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene TNNI1. In some embodiments, the sequences from gene TNNI1 comprise or consist of:











(SEQ ID NO: 1117)



GTAAGCGTCTGCAGCCCAGAACTTCAGATGTAGGTTGATCCCAGG







CAGAGAACCTGGGGCTTTTGTTTAAGGAGAAAGCGAGGTCTTGGA







GCTGGAGAAAACCATTTCTTGCCTGTGCAGTTGGTTTGTTGGTAG







AACATTCCTAAAATACTCCCACAGCATTTTGTCCCACAGAATGGC







ATCTCTCCTGCAAAACTGTTGTGAGAGGTGAAACTTTTTCCTGGC







CATTTGGAGGCTGTGAGCAAGGAGCAGAATCTTCCGTGAGGTTCC







TTAGGAGCCCCAGGGCAGAGCCTAAGGGATGATGTTTTCACACAG







GATGCCCAGCAGAGTGTGGGAGGGAGAGCGCACCCAGGGGAAGGG







GTGGCCCTGGGATCAGAGAGCCAGGGTGAGTGCTCAAAGTCAGCA







ATGCCTGCAGCGGAAGAGAAGGAGGGTGCCTGACGCGCAGGACTC







CCAGGGAGCCTAGAAGGCAGGGCCAGAGCTCCCTGCTGGCCAAGG







AAAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene TNNI1. In some embodiments, the sequences from gene TNNI1 comprise or consist of:











(SEQ ID NO: 1118)



CTCTTGGGCTTTTGGGGCATGGGTTCAGGGGCCTCATTTCCATAT







TGCCTTATAAGAGATGCAGGGTTAGCCCAATGGTCCTCTTCCCCC







AGCTGCTACTTGCCCCTCTGGGCCTTCACTGTGGCCCTTTGCTCT







CCCTCATTCCCCCTCCCAGTCCCATCTCTGCTCAGTCCCCACACC







TGGTCTGGCTGCTCATTCTTTCCCTTTCTGCAGCTCGAGTCTCCC







CAGGTGGGTGCTGGTTTCACTCAGTTGGTGGCACCTATGTGCACA







GAATTTGCCTCTGCTCCTGAGCCACAAATTCACATGGCTTTCCGC







CTATGTGCCTGTTGTGTGCATTGCAGCACGCATCTGCCCTGTGAG







GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG







AAGGGTGGGAGGGAGGAGGGGCAGCAGGAGGGGGCAGTGGGTCTG







TTCTATTTTTACCAGCCAGTTGCTGCTGGACACAGTTTTCATAGC







CTCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene OBSCN. In some embodiments, the sequences from gene OBSCN comprise or consist of:











(SEQ ID NO: 1119)



TCCCAAGGGCCCACCTCTTCATCCATAGGGGGTTAGTATTTCAGC







ATATGGTCTTTGGGGGGCATCTCAACATTTAGTCCATAAGAGAGG







GTAAGTCCAGCCTCAGTCACTCCATCTTGACTAGAAACACTAGTC







CTAGCTTCTGGTTTTGAGGCCTGTATCTTTTCATCCCATTATTCC







CTAGTAGGATGGAACTGGAAGGTTCTACCACTATCACTGTTTCTT







CTGAGGCCCCTGGATCATTCGGTATTAGTTTGTTGTGTCAAAGAG







GCTTGTAGTTAGTTATAGGCCTTGAAGAATGGGGCTAGTAATAGC







CTGAGGTGGAGAGGCTACAATGTTGCTGTTTCCCCTGTGACTTCC







TAGCTCCCAAGTCTAAGTGGCTCCCGGGCAAGGCTCTGTAGTCAG







GACCTCTGAGGGCCGAGGCCATCCTGGTGCGGTAGCTTATGCTGG







GGCAGATGGGATGGCAGACCTTGCTCCAGCTCCCCTCTTCCTTCC







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene OBSCN. In some embodiments, the sequences from gene OBSCN comprise or consist of:











(SEQ ID NO: 1120)



TAGGGGCTGCCAGAGGTGGGCTGTGCCCCATGGACTGACGTCGCA







GGGCCTCTCGGGGAGTGATGCTGCTGGGTGAGGGCAGGGCTTGGT







CTGACAGCATCCAATCCTGGAGCCTTCCTCCAGGCCCTGGCGGTC







TCTGTCCACTCAGTCAGCAAACAGGGGCTTCCAGCGTCATCCATG







CCCAAGCCCCTCAGCACCCCTCCCGCCCTGCTCCCCTCGATGCCC







TGCTTTATGGCTGACCCCTTTTCTGTTCTGCCAGCCGGGAGCAGG







AGGGAACAGAGTCCACCACTGATGAGGGCCAGCTGCCCCAGGTGG







TGGAGGAGCTGAGAGACCTCCAGGTGGCCCCTGGCACACGCCTGG







CCAAGTTCCAGCTCAAGGTGAAAGGTGAGAAGGAGAGGGAGTGGG







GACACAGCCATGGCAGGATGGAGGCCAAGGAGCCATACCCCATCA







GCCACAATTGAAAGGAGACCCCAGCAGCGGGGTCAGGCAGTCACT







CATC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene KANSL3. In some embodiments, the sequences from gene KANSL3 comprise or consist of:











(SEQ ID NO: 1121)



GTGAGAGCAGCGTTGGCTTCGAGGGGAGTGGCGGGCCGTGGGGAT







CGCGGCCGTGGTTGGGACGGCCGGGGGTCTCGGCGAGGGCTTCAG







GGCTCCCGGGGCATGCGGGGGCCCGGGCTAGGGCCGGCTGCCGGT







GCTGGTCGGGCGGGGCGGGTCGGGCCGGGGGCGGGCTGGCAGGGG







CCGCGTCTGGGCCTCCGTGACGCGGACGCCGAGCCCTGCCCCAGG







GAGCGGGAGGCGGGAGGCGGGGGCCAGGCGGCTCCTCCTCGTCGC







GAACTCTGGATGACCTTGGCCAAATCGCTTCAGTTCCCCGAGCTG







GCGACTTCGTCTGTAAAATGCGAGTCATAGCCCTGTCTCCTAGAT







GGGCCGTGTGGAGGCTCAGTGGCGGGAACGCCTGCGGGGCAGCCG







GGGTCCTGGGCGGTTTGCTAGGGACGCCTCACTGGGGGTGAGGGT







GAAAGGGCTTCCACGGGGATCATTTTGTCACAGGCTTTGTTGGGG







GGGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene KANSL3. In some embodiments, the sequences from gene KANSL3 comprise or consist of:











(SEQ ID NO: 1122)



CCTCCCGGGTAGCTGGGACTACAGGCATACACCACTATCACACAC







ACCTAATTTCTAAAATTTTTTGTAGATACGGGGGTCTCATCATAT







TGGCCAGGCTCATCTCAAACTCCTGAGCTCAAGAGGTCCTCCCGC







CTAGGCCTCCCAAAGCGCTGGGATTACAGGCGTGAGCCACATTAG







GTAAATTCTTAATGAGTAAATTCAGGTGGCTGCCCTGGTGGAGGT







GGACCGTTGGGTGCTCGACCGCCCAGAGGGCACAAAGGACCAACA







CGGTGGGGGTGGCGAGAACTGGGGCCGTAGAAGCCAGCCTAGTCC







TCCGCCCTCGCTTTCCCTACTGAATATGCGCGAGGGGGAAACTCC







AGCCTAGTTGTTGACTTTCTGCTTCGGACATGCTCGCGTGTTCTT







GGCTCTCCTCTTCCGGACAGAGGCGCCGGAGCTTCATCCGTTCCG







GGCTTTGCCGCTCACCGCCTCTCCCTTCCCAGGCCGTAACCCGCT







TCCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene NEB. In some embodiments, the sequences from gene NEB comprise or consist of:











(SEQ ID NO: 1123)



GTAGTGCCCACTTGGTCTTTTCTTTCCCTCTTAATCCAAATAATT







GCACTAGTGACACTCATTAATGTGTTCCTCCTACCATTTACATCA







CCACCTAACTGTGGGTTTCAAATTGGGTTCTCATGTGCTTGGTAC







TACCACCTAATCTATTACCCATGTTAATTCTTCTAATTTTTTATT







TGAATATTACCTTAGCATTCTATTAACTTCAAGCTTTCATTTGAT







ATTTAATGTTAACTGATCATGCTTATACCCTGCCTGTATTCATAT







TTTTACTTACTAAACTGTGAACCCTTTAAAAATTGGAATTGTTTT







TTATTTTCTTCTTTGCCTGAGAGAATCAATCTGGGCTTTCTTGTC







AATGTTGTTGCTTTTCTTTCATGATATAGAAAGAGTACAGGAAAG







ATCTAGAGGAAAGCATCCGTGGGAAGGGCCTCACTGAAATGGAAG







ATACACCTGACATGCTAAGAGCAAAGAATGCCACTCAAATCCTCA







ATGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene NEB. In some embodiments, the sequences from gene NEB comprise or consist of:











(SEQ ID NO: 1124)



CCAGCCTGGGCATCATAGCAAGACCCCATATTAAAAAAACAGGTC







ATGATTAGGAAAAAAAGCAAGTTAGCATTTGGTAGCCTTAGGCTC







TCCTTCCACTGCGATTTTTTTCCCCTTGCCTCACTGACTTGTAAT







TCTGAAATAAAGTCTTTTGTCACCTTAAGAAAGGTGTGGGAAAGT







TCAGAAGAGACAACAGAGGATTCAGAGTCCTGATGGTGCATCCAT







CCTTCCACTCTGTGAAGGGCCTGCCCCAGTCACAACATTAGACTG







ATGAGTGAGGGGCCCATCAAAGTGTCTCAGCCTGAATTTCCTTGT







GGCCATTTGCTCCAGACAAAGTATTTCAAGTACAATCATACATGT







TTCAAATGTGTGACCATTAGCAGAACTATGATTAAGAGCCCATAC







ATAGAAAATGCTGTTTTTTTTTTCTTCCTGAGATGGAGAGATATA







TTGTAATAAAGATTTTTTTTTAACTTGTCTTTCCTTCCTACTTAC







ATTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene SEPT2. In some embodiments, the sequences from gene SEPT2 comprise or consist of:











(SEQ ID NO: 1125)



GGGGGACGCGCCGCTCGTCCCATACTCTCGGGCGGTGGTCGGCTG







GCTCTGGGGTGCCGGCGGGGCGGTGTGGGGGAGGGGCGGCCGAGC







CCAGGCTCTTCGGCACTTATCGTGGGCTCCCCCGTCCCGGGCGGT







CCTAGTGCCGGCGACAGTGGCTCCGACACGGGCAGGCCCGGGCGC







GGGAGTGGGGATTCCGGTGACCCCTTGTTGACCCTGCGGGAATTC







CTCTGCACTGTCGTCGGTGCTGGGCCTCGGGGCGTCGAGTGCGGT







CGAGGTCGGGAGGTTTGGTGCTTGGAGGGAGGATACAGGTTTAGG







CCCGACAAACACTTAGAGCTTGTGTGGCCATCTTGTCTTTCTAAC







GGTGCCATTTCCTCCCTGAGGCGTGGAGGACTGGCCAGCCCCCAA







ACCTCCAAGCCCATCGGCTGGATGCCGTGGATAAGCGAGGGGAGA







GCGACTAGGCCCTGTCTGCGGGTACCTTCGGCGAGGAGAGGCGAC







GAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene SEPT2. In some embodiments, the sequences from gene SEPT2 comprise or consist of:











(SEQ ID NO: 1126)



CACGGACAGGCAGCAGGGGGTAGGGTATAGGGTTGGAGGCCGCCG







AGTTGGCCCGGGGATGAGGAGCAAACCTGTGGCTTCCGTGGTCTC







AGTGGAGTCCCAACTGTGACCTGGCCTACTGGCCCAGACAAACCT







GGCTTGCTTTTTTACCTTTTCGGTCTAATTCTTGTCAGTGCCACC







AGACCTTGTCCTCTCTCCACGGAAACACACGAACCAACTACACTG







AACTTCTTGTAGTTCAAACTTCTGCACCTTTGGCCATTGCCCAAA







CTCTTAAATCTGGCTACTTCCCACCTTAACTGCCTTCTCCTTACC







TAGTCTGTGCTTGTGGGATTCCGCCCAAATTAGATTTCCTCCAGA







CAGGCTTAGGTGTTTACCTGATGTTCCAGATGGTATTTGTCCAGT







GGACTATTGGTGCTGTGAGGGCAGGGCACATGTTGTCACCCATAC







TCATTGCCTCGGTTGGTGTCTAGCACCTAGTAGGTTCTCAAATAC







TTTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene SLC6A6. In some embodiments, the sequences from gene SLC6A6 comprise or consist of:











(SEQ ID NO: 1127)



GTTCCTTGGGACCTCCTTCTGCATTCTAACTCGGGGGTCTGCAAA







CAGTAACAACAATGGTGGTGACAGCAGCCTTGACTTACGGGCTTG







CCATGTAACTGGCACTGGGCTGAATCGCCACTCTTTGAACCTGGG







AAATAGCTCTATGGGGTAGGGCAAAGGAGCCCATTTTATAGATGT







GAAAACTATCTATAAACTGCAGGAGTGATATTTACTTGCCCACTG







TCATTCCAGGGTTGGAATCCAGATCCATCTGCTTCTGAATCCTCT







TAACCCTGATGCTAGGTCGCTGGGAGGTGGGGTGTTTGATTTCTG







GGGCTCCTTGCAGCTCAGTACTGCCTTGAAAGGCACCTTGGATTC







GAGCATAAATGTTCCATTTGCATGTGCAAGGTCCCGTCCCCAGGC







CATCGCCAGGTGGACATAACCCTCGCTGCCTCCTCCAGGAAGCCA







GCTCTGACAGCCCTCCTCTCTTTCCTTGCCACCTCCCTCCCCCTC







ATAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene SLC6A6. In some embodiments, the sequences from gene SLC6A6 comprise or consist of:











(SEQ ID NO: 1128)



CAAGTGTTGCGCCGGCGGGCCTGGTGGACTTTAGAAATGATGATG







ATGATGTTTAAAGAAAAAGAGAAGTAGGGAGCGTGGCTTCCTTGT







GTTGTGAATTAACTTGCTCCCTGACATATGTGTAACTTAGGGACT







GTATGCTGCTGGGATGCCTGAACAGTGGTACCAGTTTTGTGTCTG







GCTTCGCAATTTTTTCCATCCTGGGCTTCATGGCACAAGAGCAAG







GGGTGGACATTGCTGATGTGGCTGAGTCAGGTACGGTGGTATCGG







TGGCAGTGGTGGTGGGCACTGCCACCTAGTGTGTAAGCCAAGTAC







TGCAGGCATGAAGCCAGACCCCAGGGGGCTTTGAGGGGGGACGAG







CCTGGTTTCTAAAATGGACCCCCCCCCCGCCACCAAGATATCCCC







CAAATTTCAAAGAGTACAAAGCCAAATTTTAAACATTTGTATTGC







AATTTGGCCCTGATGGGTGGCAAAAGCATGGGGGGTGGTTAAGTT







GGGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene THOC7. In some embodiments, the sequences from gene THOC7 comprise or consist of:











(SEQ ID NO: 1129)



GTGTGCCGGGAGCGTGGTCGGTGGGCTGGGAACACACGTGGGTGG







CCGAGACGGCTAGAAGCGGTGCAAGGGCGGGTGGGACCAGAGGGA







GTCCGGGTTTGTCTAGGGGCGCGGTGAGGACCCAGGAGCCGCGAA







GGGTGGACAGTGGTGCCGGGCTTAGGGCTGGGGGCCTGGAAGGTT







TGTGGAGGTCTGGGAGGGAGTGGGAGCTCCAGGGACGGGGCGGGA







ATTTCGGGACTCGTCTACAAGGGGTGTCAGGATACAGAGCGTGAC







ACCAGGAGGGGTCCTGGGGCATATTGGGAAGCACTCACAGACAGT







GGAGTCGATGGCAATGAGGCCTTGAACTGCGAGTGGATTGTGGGT







GAGAAGGAGGAGCAGGGAATTATTTATAAAAGGAATAGATAGGGA







TGAGGTATCCGGAGGGTGATCAGGAAGTGTACCTGGGAGATTTAC







GGGGGTAAGGAGGAGCCAGTATAGTGATGAGACCGTCTAGGGTAG







ATCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene THOC7. In some embodiments, the sequences from gene THOC7 comprise or consist of:











(SEQ ID NO: 1130)



GGGGCGGCGGGGCCGCAGTCACCTTCCCGGGGAAGAAGCCGGGAC







CCCGACTGCGGGCGGCCCGCAGCTCCCGCCGCACCCCCGCAGCCC







TGGCGGCGTCCGGGCGAGCTCACCTGGCGGCTGGCTGCGGCCTCC







GGGGCGGCCTCTGGGCGTGGGCCGGGCGGGGGCTGCAAGGGGGGC







GGCGGGAGCCCGGGCTGCAGCCGGGGCCGCCGCCGGCCGCCAAGC







CTCGCCCCAACTCGCGCCGGCCGCGGCCGCCCCCGGGGAGTACCG







GGAGCGCGGGGAGCGCGGGTCCCGCGGCGGCGGCGGCGGCGGCGG







CGGCGCGGCGCGGCGCGGCTCAGGCGTCGGAGCAGGCGGCCGCGG







GCGCCGCCGCCTCCTCCTCCATGGCTGTTTACCCGGCTGCATTGT







GGGAGTTTGACCTCCGCCGCCGCCAACCGCCGCCTCAGCTTGCGC







CGCCGCCGCCGCCGCCGCCGCCGCGCACGCCATGGGAGCCGTGAC







TGAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene MBNL1. In some embodiments, the sequences from gene MBNL1 comprise or consist of:











(SEQ ID NO: 1131)



AACACGTCAGACTCATTTACAAGAATGCTAGATTGTGCATATGTG







GCATGTTAATAAGTAACCTTTGGTAGAACTATTATGATGGATTCA







ATACAATCCATCTATGGTGCAAATCAATGATAAAGAACTCTCATG







CGTCTACCATGTATGACTGTAGAATTGCTGGTGTAAGCAGAACTA







TAGCAGAGCAAATTTCAACATGCCCTTGGCCACCTGGGTTGTAGG







GGACACTTTACTTTTTGCATTGTGCTGTGACCATTTCATGCATAA







TCTTGGATCTTCATACTATTTGACAACCAAAAGAAAGAAAAGGGA







ATGGATATATATATACACACATATATTATATCTACATATACATAC







ATATGTATATGCCTACAGTACATTAGCAATGCTGGAAATAGCTGA







TGCCACAAAATGCAGCCTGCATGCATGCAGAAGTTTATAGCTGGC







CCTTCATCTGCATCGATTGGTGTTGAAGGTCCTTGGTATGTTTCG







ACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene MBNL1. In some embodiments, the sequences from gene MBNL1 comprise or consist of:











(SEQ ID NO: 1132)



TATGAGAAGCTTCATTATGCTTGGAGCACATTTTCGTGCCATTTG







CCAATGCTGTAAAATTGTGTAAAAATGTCAGCTGAGAACTGGAAA







TAAAACAGGGACATATTAATATACACGCCAGACTGTGGATTGTAG







TATGTTGAATTAGCCTAAGATGTCTAAATTGAAAGAACAGCAACA







AAATGCCTTTGTGTATCTGATTTCATTATTCTCCCAACAACATTG







CTGAAATGGGATTAGTATCATTTTATAGAAGAGACTTAAGCATAG







AGTAATGAGTTGCTTTGCTCAGGGTCACACAGCCAGTAAATGGAT







CTCTGGGGCTAGCACCCGTGTCATCAGTCCTCTGACAGTCTCAAT







CCATTGTGCTGCCTCTCTCAGGTTAGCAGGCATTAATTTCAGGTT







GAAGAATCAAGCTACAAATCCGAAGTTCTTAACTTTGGGAACTAT







TGAAATAAGTCAACTCTATATTGGTTTCTTATTGTATTTTCTCTC







AAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene SPARCL1. In some embodiments, the sequences from gene SPARCL1 comprise or consist of:











(SEQ ID NO: 1133)



GTACGTACCATCTTACTCCTTAAAATGTTTGGAACTTTTTGTTTA







AAAACTCAAACTCAAAGTGAAATCTCAATTTGTCACAATGGTGGT







TTTTTTGTTTGTTTTGGGATTTTTTTAGTTTTTGTCCCCCTTCCT







TACACCTTTGTGTAAGAGGTAAAATGGAGAATTTGCAAAATAACT







AGAAAAAAGCTGGAGTGGGAGCCAGGAAATCTAGCTTCAATCCCA







GCTCCCCTTCTACAGAGCTCAGTGATCTCCAGCATGTCACACTGT







AGGCTTTCTAGGCCTGTTCTTCATCTGCCACATCCTGGGGTTGTT







GTAAAGATCAAATAAGGTAATATCCCATTTGTAAATTTATTTGGT







AAACCCAAAGCACTATGGCAATAGAAATGTCAAGTATATTTTATA







TGCCATTACCCGTTCTAAATAAAGTAAACACCCATTCCATTGTTA







ATATTCTAAATTTCTCCTTGTTATATGTAGTCATTTTCAGAAGGA







TCAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene SPARCL1. In some embodiments, the sequences from gene SPARCL1 comprise or consist of:











(SEQ ID NO: 1134)



GCATTCACTAGGAGGATAGAATGTTTTAAATTATCATCGCTAATA







GAGTCCACATATTTACTTTAAGCATGAATTACCATATATAAAATA







TTTTATTTTAATACAATAAAAATCATGGAATATATTTTAGTGGGT







TTAGTAGTTTAAGGGGAAAAGATAGCAGCAATTTAGGATTCATAC







AATTAATGGAATTATTTTCAATTTTAGAATCCTAAAATATTAGGG







TTAGAGGATCATCTAGGTCTCACTCTTCATTGTTCATCACTTGAG







AAAACTGTATCTAAGAAAGGGCAAGTGACTTTCCCAAGGTCTCCT







GGCAGTCAGGGACACTGCTAGAGCCAGAATCCAGCTCTCCTGACA







GTTTAATGCTTTTCTTGTTTTAAGGTGTTTTTCAATGTATTAGAG







TTTTGGGGCAGCGGGGGGAGGTTGCTCCATGTTTTTACTTAAATA







CATTTTTCTTTCATGGAAAATTGAATTCCTATGCTTTTTGCCTTG







TTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene PALLD. In some embodiments, the sequences from gene PALLD comprise or consist of:











(SEQ ID NO: 1135)



AAAAGAAATATACTGCATGATGATTTGTGCTTTTCGATTTAATGT







CACACAATCGTTTTTACACTGTATGTGCTTTCCCTCCCCACAGCC







CCAGTCCCAGCCCCAGTCTCGCAGTTCCATTCCTTCAGGGCAGCT







TCCATTCTACCGTGAGTTTTTATTGCTTTCCCCTAAAACGCTGCC







CTGGAGTCGCGTTTATTGGACACTCTTTAGTCTGTAAAGGAAAAA







AACACAAACACCGGAGGAGAATCATCCTAACAAGCCGACAACATA







TCTATGGGCCTAAAAAACTTAAATTCTTTCCCGTTATTAGCACTT







CTCGATCGTTCCAGGAAAGCCCGATTTGTAGCTTGGCTGCCCCAG







GAAGGGGCCTGCGACCAGAAAGATGCAAAGGGCGGGACAAGGTTA







CGAGTGGGTGCGGGGAAGCCTCCCTAACCTGGGGGCCGCGTCCCA







GAGCTGCGAGCCACGCCTCCTCTCTCCCGCCCGCCGCGCCGCCGG







ACTC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene PALLD. In some embodiments, the sequences from gene PALLD comprise or consist of:











(SEQ ID NO: 1136)



TAGGCGCGGGGAATCGGCCCTGAGGCTGGTGGAAGAAATGTGTGA







ATTAAAGGGAGTGCAGCGTGCAGGGTGGGCCGCGAGTCGGGAGTG







CAGGGCTCGGGACAGGGTGGGGGCGGGGAGGACAAGAGACTCGTC







AGCGCACTTTCCACCCGGCCGGTTCCTTCCCGAGCCCTCTCCCTC







CCCCGGCCCGCGGGAGCACAGATCCCTGAAAGCGGAGCTCCAGAT







AGGAATGTCTGCATCATCTTGGCAGGGCCCAGACGGGAGAAGATG







CTCTGCTGCCACGGACGATCCCTCCCGCTCTCCTAACTCTTCTCT







GAACACCTCGCACCCCCACGGCCCCCCGCCCCTCTCTTAATCTTG







GCCACGCTCCCTGAGGGGTCAGACCTGGGGTGGGAGAGCAGAGAA







AGCGGGGCTGGCAGAGACAGGGGTGAGGGCCGGGAGAGGCGATGA







CATCATTCCCTGGCAGCTGGCGGGGAGAGGGTGGGGACGGAGGGG







TCCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene ASB5. In some embodiments, the sequences from gene ASB5 comprise or consist of:











(SEQ ID NO: 1137)



GTATGTTTATGATAATCCTCTCTTGTTTCAAGTGGAGTTTAACTT







TCATCTGATCCACAATTACATGTTTTCTTTTTAGACTGCTTCAAG







GTAAATATTTTATAAACTTTTGTTAAGGGAAATACTTGTAACTCA







CTTCGTGGCTTTATAAAAAGGAAAATTACTTTTTTAATGTAAATG







TTTGGGTTAAAATCAAGGTATCATTGCAAAAGAGATTAAGGAAGG







CATACTATATATATAATAGGAATATATATATGTAATAGAAATATA







TCTATTCCTATTAAGAAACATTTTAAAAAGTTAAACATTTGATCT







GCCAAGTCATAGTATTAGAGATTAGTTTTAGAAGTAATTGATTGC







ACTTCAGTCGTCCTCTAATGTGTACCTGGATTGTATAATTTTAAA







TTAAATCTTTAGTTATGTGTAAAATAATTGTTATTAAAAAGAGGG







GATTTCAAGATTTGGTTTCTCACAACTTTAATAAGATAACCATTT







GACT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene ASB5. In some embodiments, the sequences from gene ASB5 comprise or consist of:











(SEQ ID NO: 1138)



CCATTGGATGAAGGAACCGTGTTAAGTTATTAATCTGTGATGTTC







CAGTGTAATGACTTGTAAGAAAAATGTTTTTTTAAATCAGTAAAA







ATAACATAATTGGTTAATTTGATGAGTAGTCATTAAAATACAAGC







CATGGACTGTGTACTGATTTAGCTCATGGTCTACAAATAGTACAG







TGCCATTTTAAAGCCTTAAATGATAGACAACTTTACTCGATATGA







ATATCATTTACATTAACAATGGGAACCCCATACACTGTCTTCAAA







TTGTTCTTGATAACTAAATATATAAAATGGAAAGGATATGCATAT







ATATTTCAATATCTGGATTTTGAAATTTGGAGCACAGAGGTGGAG







TGTCATTTTATATTGAGAATGGAAAGTCAGGAGACTTGTGACACG







TGATGCAATTGCTTCCATGGAATATTTTCAGACCAATAGGAGTTT







GCGAAGAACTTTTATGGAGTGTATAAATATTTTGAGCTGTAGGCA







TCAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene CMYA5. In some embodiments, the sequences from gene CMYA5 comprise or consist of:











(SEQ ID NO: 1139)



AATATTACTCATTTTTTAAAATTGGAAAATATAGAAAAGCACACA







AAATATCACTCAGATAAATACTGATTACAGTTTTGATCTCTATCC







TTCATTGAAAACATTTAAGTCATTTTTCCTGCTTTAGAGAATTCT







ATTCTGGAAGCTAAGCCATTGGTTGATGAATAGAGAGAAGCAGAG







CATAAGCAACTCATTGCCAGTCCCGCTGATCACAGAGACAGAGCC







AACTGCAAATGAAACCATCCTCCATCCCTGTCCTGACACTGCCCA







TCCCACCCATTCCCCCTCCCCTGCACCCCGCCTTGTGAGCACTTA







TGTCCTTGCTGCCCAGACTGAGACAAAAACAACCTCAGGGAAGCT







GAGGGGTTATGATTTTCAAACGTGGGTAATCTTAATGCTCCTGGT







TGTTTTTTCTTTCTGTTGTTAATTAGCTTCTCTCTCTCTTTCTCT







CTCTCTTTTTCTCTCTTCTCCTCCTCCTTCCTCTCTCTTTCTCCC







TAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene CMYA5. In some embodiments, the sequences from gene CMYA5 comprise or consist of:











(SEQ ID NO: 1140)



TATATAAAACATGATACAATGTAGTGGCAAACAGCATGACTGGAT







GCTTGCTTTTTAATATTTTGGTGCTTTATGGAAAATGAGCTGCCA







ATTAAAAAATGCATTTAGTGGGCTACAGTATTTTCACTTTTAGAC







ACAGTGCTTTTAAAAGCTAAATGACAGCTTTGAAAGAAAACTGCA







TGTGCTTTAGTTAAATAACAATTATCATAATGTAGATTTTTAACT







GCTTCCTCAAGATGTATTCTGCATTTTCAGCCTAATCTAGGCCAT







CTTTCCTGTCACTGCATATAGAACATTTTGTAGATGCTTAGATGC







ATTGGTTAAACATGAGTATTTGAAAGAATCATTCATATCTGCCTT







GAGTAATTTTATGATTTATGTAATTTTAATGAACTGTTCTATCAT







TTTCTCTTCAAAAGATAGTCATGCATTTATCTTCTCTTTCTGCTC







AGCTAACCTAAATGAATCTCCCTCCTTTGATTTTGTTTTGTTTTG







TTGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene MEF2C. In some embodiments, the sequences from gene MEF2C comprise or consist of:











(SEQ ID NO: 1141)



GTGAGTGCTCACATTTGTTTTTATCATTTACCACAGAGTTACCAT







AAAGGTAAAACTGCCAAACTCCTTGGCCTTAGGTATTTACAGAAT







AGACGTGTATAACAATAATAAAGAGTTAAATGACCACCATACACT







TTATATTATAAGCAATGACATATCATTTTGCTTAAAGATCATGTC







ATCTTTCAGGGCCTACTTTTGCAAGTACTGTATCTTATAAAATTA







ATCTATTTCATGGGAAGTCCTGTGTATCATCAATACTATAATTAG







ACAAAGATTAAAATATGTAATGCTATTGGAATTTGTGTTTCACTG







CTTTTTAGATTTTTCTAAACCACCATGTTCACTTATCATGTTTGT







GTTAAAAGTATTCTGGCTGAGGAACAAATACTATACAGTTGAATA







ATCTTAAGGGGTACATTAATCTATAGCCTTTCTTCATCTCAGGGT







GGGTTGCTGTGGCAACCGCCCCAGTTCACTTTAAAATAATTTTTA







AAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene MEF2C. In some embodiments, the sequences from gene MEF2C comprise or consist of:











(SEQ ID NO: 1142)



GGCCTTTAGGCAATGCATAAAATAAGCCCTTTCAGTAACCTCAAC







TGCATTGCAAACATTTCCCGATTTGACAAATTGTGTTAGCTTCGG







ATATAATGGATTTGACATTCTTCTGTAATTCTCTATCAGATATAA







AACTGAAGGTACAGAAATACAGATAAACATAGAATCCTTAGATAT







GTGTAAACCTTGTCTTGAAATAACAAATCCTTTGTATATTGCAAA







TTCTCAATCACAGACATTCCACATGCTGTATATTCTTCTTGGGTT







AATGCTGCACCTAGTGAATTTTTGAAATTTAGATATTTGGCACTA







TTTATGTTAAAGATTATTATCACAATGCATGACAATTTCAGTTGT







CTTATATTCAAAACACTTTGGTTTAAAATCAGAATATTGTTACTT







CAGATTAGAAGTTTTAGAAATAAATCTATTTGAAAGTATTTTCAT







ATCAGCATTAACAAAATACTATATACTGTTTTGCACTTCCACATT







TGAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene CSNK1A1. In some embodiments, the sequences from gene CSNK1A1 comprise or consist of:











(SEQ ID NO: 1143)



GAAGTGGCAGTGAAGCTAGAATCTCAGAAGGCCAGGCATCCCCAG







TTGCTGTACGAGAGCAAGCTCTATAAGATTCTTCAAGGTGGGGTT







GGCATCCCCCACATACGGTAAGAACCCGGTGCATATAGGCGATCC







GCTGAGGGAAGCACGGAATGGGGAAGTGGGTAGAGGCAGGTAATG







AGACCAGCTGAATCCGGGTCTTGGTGTTTTAGTTACCTGGGTTCC







CTATAGCTGGTGGTTTCCTGTAAGTCAGTCATCTCAATTACTGAG







TAAATTTCAGTTTGCCAACTTATTTCATTGTATTACACTGCTCCA







TTTCGCTTCCGTCATTCTCACTCTTCCCGAGGCTTTCATTTGCAG







TTATGCGTGCAGGTCATCTTTGATCCCTACGCGGAAGGGTTGGCT







GCTGCCTTTTTTACGGCGGCACTTCTGATGTACTTAGGTTTGTCC







AGTGAAGTGGATTTGAAGGTGCCGAAGCGACCCGGCAGTGTCAGT







TCGA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene CSNK1A1. In some embodiments, the sequences from gene CSNK1A1 comprise or consist of:











(SEQ ID NO: 1144)



GGGGTGGGGTTCACGATAACGCTGCGCTTTTACTCATTCCCCCCA







CCACCACCGTGCACCCCCAAAGTGCTCGGGCCTCTCCTCATCGCA







CGCACTCGCCAGGGAGTCCAGGGAGCTAGTTTTCCTGGCTTACGA







ACCTCGTCCGCTGTCTCCCTAAAAGGGTCAAGAACCCGATAGAAA







GGTTGGGGGAACTAGTTTCCCACCTTGTTAAACACGTCCCATAAA







TGTCCCTTAGTCGAGCTTTTTTTTTTTTTAACCGTCTTTTAAGGT







GGAAACGGCAAAATCCTCTTTCAGTGATTCCCATGTTTTTTTTTT







TTTAATTCTTAATTCTACTGGGCCTCTTGTTCCAATTAGAACAGT







TTCCGATCGCCTAGTTCCTTAAAAACCTCTCACTACGTTCAGAGG







TTGCCTCTTTTTTCTGGATGAATCATTTTCCTGCACTTTAGAGGA







CGTACGGATTTCGTCACTACCCCCTGAAGAGGCCTTTGAGGCTGG







TTGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene TRDN. In some embodiments, the sequences from gene TRDN comprise or consist of:











(SEQ ID NO: 1145)



GTAACAGAGTTAATTTAATATAATATTACAAATTTATTAGGAAAT







ATATATTATTGAGAACACAGTACATTAGCTGCATCCCCTTTGCAA







ATATGTAAACCAGAACTGCAGAGGTCACACACATATTTTTAAAAA







CTTGTGTTTATTTGTTTGTCTCTAAAATTATTTCTTCTCATTATT







ACCAAGAAAGTGCTCTGATTACAATTCCCAAAAATCCCAAAGAGA







AAAGAAAGAAAAATGAAAAAAGGTTTTTTTAATGAAAAAGATAAA







AAATATAGAAGGGAAGTAAAGAATATTAAGAAGCACTTGTCCCCA







AATTCTCTGATTCTCTCCTTTCTATTCCTAAAATATATATTTAGT







GCATAATATATCCCAAGCACTTGGTAAACATTGGAGATGATGATG







ACAACAAAAATGGCAAAGCTACAAATAGGAAGTAATATTTGGTAA







TTAGAACTCTGCATTCTGATATTTTCAAATGCTCTGAGGCATATT







CTGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene TRDN. In some embodiments, the sequences from gene TRDN comprise or consist of:











(SEQ ID NO: 1146)



TACTGGAGCCAGTACTTATGTGCTTGAGAGCATAGATATAGTGGT







TCAAATCCAAGGTCTCCTATTTAAAGATCTTGGAAAGTTATTTAA







TCAATTTATGTGAAAAGTAGAAATAATAACAGAACCTATATACCT







CATGGAGTTGTGAGCACTGAATGAGAAAATGGTAGAGAATATTGC







CTGACACGTAGTAAGTAGACAATGTTAGCTATCATTCTTATATCT







TTCTTCTTCAAAGCCCATGTTAGTTTACATGTACCTTTTTTTGTA







TATATGGCATAGTCTGTACTAGTGATAGAAATTCATGGGCTTATT







TTCTATTGTTTACATATTTAGTCCAATGTCTTATAGACAACATAT







GCCTGGAGACATTTGTATTGTGAATATCAACTCAGATGCCCTGTG







ATCTTAAGCCTCAGTATACAAAACACATGAAGAGACTTGCATTTT







TTTCCTCTCTTATTCCTTGTAACTTTTTCTTCCCATGATCTCTCT







TGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene CCZ1. In some embodiments, the sequences from gene CCZ1 comprise or consist of:











(SEQ ID NO: 1147)



TTGCTATAATAAAGTGAGACAGGATGATATCCATTTGCTGTCTTT







GTAAACGTGTGTCAATGGAAATCGTCACAGAATAATGGACAGTTT







CTGCCAATTCTCTATTCTCTCTGGACTATACACAGAGAGACTAGA







ACAGTGAACCCCTAAGTCCCCATCACACAAGTGAATAAGATTCTG







GTCTTCGTTGCTTCTCCATGTTGTGTTGTCGGGTTTGCAAGAGCA







TTTTAAGTCATCACGTCAGTCACGAACACTTCCCTGTGCCTCTCT







GATGAGGACATCCGTGGTAACCTCCCCACTGGCGCTGTCACACCT







GACAAGGTCAACAGCACTCCCCTCGCATCATGGAATGGGCAGTCT







GTGTCCAGATGGCTCTAGTTGACCAAAAAGCAGCTTCTTACAGTT







GACTTCTCCCCATTGACTTTGAACAGATTGTTATTATACGAAAGT







CTCCTTTTTTCCACCATTTCATAACCTCTGGTTTGTTCTGTCTTC







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene CCZ1. In some embodiments, the sequences from gene CCZ1 comprise or consist of:











(SEQ ID NO: 1148)



TGGGTACGCGCCTTTGTCTGATTTCAGCCTAAGCTTTTTGTACTT







TGCTGCATCCTATTCTTAAGTGATAAATATGTTTTGGGGGTTTCG







GTTGTTTGGGCTTTTTTTGTTGGAGACGGGGTTTCACTCTTGGAG







TGCAGTGGCATGATCATAGCTCACTGTAGCCTCGACCTCCTGGGT







TCAAGTGATCTTCCCACCTTGGCCTCCTAAGTCGCTGGGACTGCC







AGTACACACCGCCGGACCCAGCTAATGTTTTTGTGGTTGTTTGTT







TAGACAGGGTCTCACTATGTTGCCCGGGCTAGTGTCAAACTCCTG







GGCTCAAGCGATCCACCCATCTTAGCCTCCCAAAGTGCTGGGATT







TCGGGCGTGAGCCCCCACACCCAGCCCATTAAATGATAAATGTGT







TGTCATAAGCAATATAGAGGCATAGAGAGGAGCCCTGTTCCCCAT







CTGGGACCCCCCTGTCTCCTTAACTAGCCAGTGCTCATATTTTTT







AAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene GTF2I. In some embodiments, the sequences from gene GTF2I comprise or consist of:











(SEQ ID NO: 1149)



TATGATAAAAAATAAATAAATTAATTAATTTAAAAAAAAAGACCC







CAAATGTTTGCTTTTAACAAAACTGAATTAAGAGAATCACTGCAG







GCCGGGCGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGG







CCGAGGCGGGCAGATCACGAGGTCAGGAGATCAAGACCTTCCTGG







CTAACACCGTGAAACCCCATCTCTACTAAAAATACAAAAAGAAAT







TAGCTGGGCATGGTGGCGGGTGCCTGTAGTCCCAGCTACTCAGGA







GGCTGAGGCAGGAGAATGGCGTGAACCTGGGAGGCGAAGCTTGCA







GTGAGCCGAGATCGCGCCACTGCGCTCCAGCCTGGGCGACAGAGC







AAGACTCTGTCTCAAAAAAATAAAACAAAACAAACAAAAAAGAAT







CACTGCAAACAAGAGTACTTTCTAGCAAAATCCATTCTGATTTGC







AACAGCACTGATAAATAACATGGTTATTGGGTTTCTTTTTGTTTT







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene GTF2I. In some embodiments, the sequences from gene GTF2I comprise or consist of:











(SEQ ID NO: 1150)



TAAGTAAAAGCCAGTATTTATGTCTTTAATAACATATCAACAAAG







GGCCATGTCTGAATGAAGTATAGAAGTTCGGGACCAGCCGGGTGC







AGTGGCTCACGCCTGTAATCGCAGCACTTTGGGAGGCCAAGGCGG







GCGGATCAGGAGGTCAGGAGATCGAGACCATCCTGGCTAACACGA







TGAAACCCCATCTCTACTAAAAATACAGGAAAATTAGCCGGGCGT







GGTGGCGGGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAG







GGGAATGGCTTGAACCCCAGAGGCGGAGCTTGCAGTGAGCCAAAA







TCGCACCACTGCACTCCAGCCTGGGCGACAGAGTGAGACTCTGTC







TCAAAAAAAAAAAAAAAAGAAGGAAAAAAAGTTCAGGACCTAAAG







AAGGAAGGTCCCAGAAACTGGGTTTCTGTTTCTTTCTACCACTAC







ACTTGCTACTGAAACCAAGCAGATGACTTCATTTCTCTTGGATTC







CACT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene POLR2J3. In some embodiments, the sequences from gene POLR2J3 comprise or consist of:











(SEQ ID NO: 1151)



GTGAGTCAATTAAACCTCTTTTCTTCATAAATTACCCAGTTTCTC







ATAGTTCTTTATAGCAGTGTGAAAACAGACTAATGGACCCTTCTG







GTTGAAGGAATGCAGCCATTCTGCTTGTTTGACTATGTCCTTTCT







ATTCATCTCTATTTCCTGGGAGGTGTTTATCCAAGTGCAATAGGA







GGTATTGGTGACCGCACAGTCCCCTCAGTGTTCTGCTAGTAAATA







GTTGAAGGTTGATCATTGATCTTCTGCGTTTTCAGTCTGGCATGG







AAAAGCCCCTGTGCAACTGGTAAAGATATCAATAAGCACCAGGAG







GTATCTAAATCCACCAGGAGCCATAGGCATCACGTTGACATCCAT







TTACCAGTCTTCCCTGGCAAGATTCTTCTGAATTGTGCTGCCTTG







GCCAAAAGAGGTATGGGAGGGGCTGGGCGCAGTGGCTTGTGCCTG







TAATCCCAACATTTTGGGAAACCAATTCAGGTGGATCGTTAGAGG







TCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene POLR2J3. In some embodiments, the sequences from gene POLR2J3 comprise or consist of:











(SEQ ID NO: 1152)



GAAAAGCCGAGGCAGGGTGATCACGAGGTCAGGAGTTCAAGACCA







GCCTGGCAAACATGGTGAAGCCCTGTTTCTACTAAAAATACAAAA







ATTAGCTAGGCATGGTGGCATGTGCTGTAGTCCCAGCTACTCGGG







AGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGC







AGTGAGCCGAGATTGTGCCACTGCACTCCAGCCTAGGTAACAGAG







CAGGACTCCATCTCAGTCAATCAATCAATCAATCAATCTCAGCGG







TTGAACTACCCTTGACATGGTTCAGCTCTGTATCCACACCCAAAT







GTCATGTCAAATTGTAATTCCCAGTGTTGTGGGAGGGACCTGGTG







GGAGGTGATTGGCTCATGGGGGCCGACTTCCCCCTTGCTGTTCTC







GTGATATTGAGTGAGCGCTTGTGGGATCTGGTTGTTTAAAAGCGT







GCAGCCCTCCCACTTCACTCTCTCTGTCTCTCCTGCTCCAACATG







GCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene ATP6V1F. In some embodiments, the sequences from gene ATP6V1F comprise or consist of:











(SEQ ID NO: 1153)



ACAGGGTCTCACTCCTGTCACCCAGGCTGGATTGCAGTGGCGTGA







TCGCAGCTCACTGCGGCCTCGACTTCCCAGGCTCAAGTGATTCTC







CCACCTCAGCTTCCTAAATAGCTAGGACTACAGCTTCCCAAGTAG







CTGGCACATGCCGCCACATCCAGCCATTTTTTTTTTGTGTGTATA







TATATAGATAGATAGATAGATCCGCCACATCCAGCCATTTTTTTG







TGTGTGTATATATAGATAGAGAGAGAGAGAGAGAGAGAGGGAGAT







GGGGGTCTCACTATGTGGCCCATTCTGGTCTCAAACTCCTGGGTT







CAAGCAATCCACCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAG







GCGTGAGCCATCACAGCTGGCCTTCAGTATCTGCACATAAAAAGG







GATCTGCATACATATAATCTTATCCTTCCCCTTTCTGACACATCA







CTGCATATTGAATACACCAGTGTGCACCCTAATCAATCTTCATTA







CCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene ATP6V1F. In some embodiments, the sequences from gene ATP6V1F comprise or consist of:











(SEQ ID NO: 1154)



TAGGGTTGACAGAGGGTTGAGCGTGGCAGCCTTTCCCCTTGTCCT







CTGTGCCCTCTGGGTCATGCTCATTCCCCTCCACAGCCCAGCCCA







ACAACTCGGAGAGGGCTGCCTGGGTAGGAGAGACGGCAGCCCCCA







GAGCTGTCCTGGACTCCCTTCTCATCTCTCCCCACAGGCAATTTC







TAAACCGGGATGACATTGGCATCATCCTCATCAACCAGTACATCG







CAGAGATGGTGCGGCATGCCCTGGACGCCCACCAGCAGTCCATCC







CCGCTGTCCTGGAGATCCCCTCCAAGGAGCACCCATATGACGCCG







CCAAGGACTCCATCCTGCGCAGGGCCAGGGGCATGTTCACTGCCG







AAGACCTGCGCTAGGGGACTCCTCATAGCCCTCAGCCCTTCCCTC







GTTTCCAGGCCTCTCCCCAGGCTTGCCATCAGCCTTCTTTACTTT







TTGAGCCTCTGATTTCCAATTCCCTGCTCCTTCCCACTCCATTAA







GAGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene ASAH1. In some embodiments, the sequences from gene ASAH1 comprise or consist of:











(SEQ ID NO: 1155)



GTGGATACTCTTTTATTCTTATGGGGAAATGGAGGCGCACAAGTT







AAATACTGAAATGTTTTAGAAAACTGTTTAACGAGAGGTAAAATG







TGCAATTATGGCTA







AGCTTTAGTCATTAAAATAATTTGGTTTATTAGATTAACCAGTTC







TCTCAAATTCTAATTAGTGCAGGTTTGATGGTAGAGAGGTTTGTG







GGGTCTCATCATCCCCACTTCATTGTAATCCCTTCATTGTCCGTT







GTGTTGTGTTCATCCCCTCTCATCGCGTCTCTGAGATCCATGAAC







CTACCCAGGACTCTAGGCTGAAAAAGAATCAGGAATTTATGGCGG







GTATAGAGGCATGATTTGGGATTGGTTGGATGTTATGAGGTGAGG







AGCAGCTGATTGGAGTTCATAGGGATGAGATGCATATCTCCAACA







GGCCACTGAGTTATCTTTGCTACTAGACATAGAAATCTAAATGCA







CAGTTGAAGGGAGAGGGGAGACTAGTAATACCTGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene ASAH1. In some embodiments, the sequences from gene ASAH1 comprise or consist of:











(SEQ ID NO: 1156)



ACTGTGGTATTTATGTACCTTTGAATGACAAACAGACGTTCTGTT







CCTTTGAGATCATCCTTTTCAATTAAGTGCGTAGATTTGGGCAGC







ATCCATGGGCAGATATGAGGGAAGAAAAAGATAACCTGTTTGGCC







AGCATGACTAGTCAAATCAATGCTGAAGATAAAGGAACTGATAGC







CAGATCACCCCTGAGTCCATCTGAGTCCCATTCAGCATTTCTTTC







CCACTGCTCACGGGATGCCTAGCACTTGCTCTCTTATTTGTATAT







GTGTGTGTTGTGTCACTTGGACTAGTTTCCATGCCACCTAGGTAG







CCATGTGGGTTCAGGTGATGTTGCTGATATTATGAATGGCATGGA







CATTGCAAACACTAAGTCTCAAAAATGATGAAATGATATTTCTTG







TTTGCAGTGAAGTAGGAAAATAAATTGTGATGACTACTGTTGATT







GAATACCAATGCTGCTAAGTGTTTTTTTTATTTTTGCATTTTTCA







TATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene CA3. In some embodiments, the sequences from gene CA3 comprise or consist of:











(SEQ ID NO: 1157)



GGCAAGATAAATTAAGTATTGAGAATGCCTTCTAGACATCTTCCC







CAACCAAATGGGGAAAGTTGAGTATATTTCTTACAGATGCAGGCA







AGGCTTTCAGTTTGACCTTTGCACATGGGATTATTTCATACTTTT







GGTCTTGAATAAAACATTTTTTAACTGCTGCAAATGATTGTATCC







TCTTGCCATTCTCTGTAACTCAGCATAGAAAGTTTGATTAACCTA







ACTGGTGCTCATGTTCTAAATTAAGGTCAGGTTGCCACCAGGTTT







CAGAGCTCTGGTTGATAAAAGGAGATAAATAAATAATTTTATATG







CTTTTTATTTGAGCCACTAGAAATGTTACCAAAATGTTACATGCT







ACTCTTTCAAATTTTCTCAGCTTTTATGAGAGTGCTCAGCAACCT







TAGCCTGTACAATGATTGGGTTTATTGTGGATAATGTTGTACTTA







TTTCCATATTCCAGGAATTTACCTTCTAATCTGTCTTCTATGGTT







TCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene CA3. In some embodiments, the sequences from gene CA3 comprise or consist of:











(SEQ ID NO: 1158)



TAAAGTAAAAATTGACTATATATTTTCTTCCAAAATGTATTTCCC







TGCCAATGGTTAGCATAGTTTGAGTTTCATTTCCTCATTTTTACC







TTCCTCCTTTCATATGCATTTTATTATTCATTCAACTAATCAAGA







TATTTCTGCAAAGATCAGCTCAAAATGTCTTACTTTTCTCTAAAA







GTGTATCTTTTACCACACTCCAGGACCCCTAGCACTTCTAAATTT







TCCCCAGATGTGGGAGGTGTGTGTGTGTGTGTAGGTGTTGGGGAC







AGAGCCTTAGAGTGAGCTTATTACGGTGGTGGTAAGGGCAAAGGT







GGCATATATTTATTTTCCTTTCTCACTGCTCAACTTCAATTGCAC







TGGAAACCAGAAAAGTCAGTGTTGGGAACCATATGATTCCAAGTC







CCTGGCTGGACATCCCCTCGGCTTGAGGTGGTCACCACTCACGTG







ATCCCAGGCTAAGATAGCACATGGAATGTGTGTGTTGTTGAGCCA







ACCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene YWHAZ. In some embodiments, the sequences from gene YWHAZ comprise or consist of:











(SEQ ID NO: 1159)



GTAGACCGGGGCGAGGCTGCGCGCCGGCGGGAGGGGAGTCCACTG







CGGGGTGAGGTTTTGGGGTGGGGCTCGCCTGGGACCTAGAGCTGA







ATTTCCTGGGCTCCGCGGTGGGAGGAAGGACTTGAATTTTCCTGG







TGGGGGTGGGGGATGGGTCTGACTTTTTTTGCTCCTTGTGAACGG







GCGGGGGAGGTGTGGACGGGGGCTGCAAGTCCAGGTCGGGGTAGA







GAACAGGGTGGTGAGCCCGCAGTCGGCTCCCGTGCTTGATGGACT







AGAACTGGAGGGTCCCTGCGCTCGGTTTGGGGACGCATATCCTGC







TTTGGGGGCGGGTGAGCCTGAGCATAGCCGCTGGTGGCCCGCTCG







GCTGGGTGTGGCTCTCGTCCCCTCCCCCACCTGCAGCTGTCGGTC







CCTTAGAGACGCTGTACACGGTCACCGGCGCTGATCGGTGGCGGG







GAAGGCGCTGGGTGGGAGAGCGGGGTGTGTCCGCGGCGGGGCTGG







TAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene YWHAZ. In some embodiments, the sequences from gene YWHAZ comprise or consist of:











(SEQ ID NO: 1160)



CGCCCCGCGGTGGCGGCGCCGGGCTGAGGCGGAGTCTCCTCAGCG







GACACGGGCGGCGGCTCCTCTTCCGCTGGGCTCGGGAGGGCGGCT







AGCGGCCGCCTCCGGCTCCTTGCTTCCGCATCGACGCCCCGGCGC







CGATCGGTGCTCGCTCCCCCGTCCTGCCCCCGGGGGTGGGTAGGG







CGCCCACACGCGAGATCCCTGGGGATCTCCCTGTCGCCGGCTCCC







GAAGGTGGACGAGGCATGGCCGGGGCGCCGCCGGCCGCGATCCAT







CCCCACCCTTGTCTCTGCGGGCTTCCACCCCCTCCCTCGCCGCCC







TTCTCGCTTCCGCCCGAGATAATGACTCGGCGGCGGCGGCGGCGG







GCATCGCAGTGGCGCAGGTGAGGCCGCCCCCTCCCCTCTCCCTCG







GCCGCCCCCTCCTTTCCCTCCCCCTCGCGCAGCCTCCGCGCTGGG







CGCTGCGGCGGCGGGACCCGGCCCTCCCCCACCCCGGGAGGCGGT







GGCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene SMARCA2. In some embodiments, the sequences from gene SMARCA2 comprise or consist of:











(SEQ ID NO: 1161)



TAATTGAAGAGGGAGCTAGTTTGCATCTCGTTCTTGCACACCCTG







AGTCATGAAAAACATTTTCTTTTAAGCAAAATTGAAAGAAAGAAA







AGAGAGAAAGAGGGCCAAAAAAAAAAAAAAAAAAAAAAAAGGTTG







CCATCCAGTGGAGGAAGGAGTAATTCCCAGCTTCATTCAGGAAAA







ACGCAGGCTGAAATATGCTTAACTGTTTTAACTTGACTGGGGATT







TTTACCACCATATGGGTTTTTTTTCGTGACATCCGGAGCCAAACG







GGTGCAGTGTGCCTTTAAGAAAAGAGGTGCCTCACTAAACTTCGC







TGTAGTTGTGTTTCACCGTCTCGGTAGGGAAGGAAAGTGTTAATT







TCTTATTTGTACACTTTTTTTCCCCGACTTCCTTCCTATTCACTT







CATTAAATCTAGAGGCAGTTGAGCATGGGAGCCGTCTGTATGTTG







AATTAGGGCTCGCACTCTTGCGCAACACGTCACCAGTCGGAAACT







GGGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene SMARCA2. In some embodiments, the sequences from gene SMARCA2 comprise or consist of:











(SEQ ID NO: 1162)



TCAGTACTTTGTGTGTTTCCTTGTAATTCCAAAACCTAAATTTAA







TAAGAATCTGCACGTATTAGCAGTTGTAATAAAAATTGTTTCAGT







TGTTCTGTTTGCAATTTAATTTGTTTTCGCTTCTTAGCTACTCAC







AGATTTAGAGATTTAAAAGTCCAAATATCACAAGCTTATGCTTTT







TCCCTCTTTTCAAAATTAAGGCGGGGTGAGAGTAGAAATATCCTT







TTTACAACTCCCCCCAACTTCATTTATCTCAGTGAGAAGAAAAAC







AATGAAAAATTTTACAGCATGAAACTGAAAGTGAAGTGTTACAGT







TGTTCTGAATTTTCTTTAAAGAGCAGAATAACATATTATGTTAAA







ATATTTGAATCTTAGAACATGTACATTGCTTTTATTTACATGCAT







TTAATTTTCTTAAAAAATTGTCTTCTGTTTTAAAAATCATTTAGA







CTGCTCCAAACCATGCTGCTATTTTTTAAAAAATAAGGTACAAAA







TACC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene PLIN2. In some embodiments, the sequences from gene PLIN2 comprise or consist of:











(SEQ ID NO: 1163)



AGTGTGGTGACTCGGGTGGTCAACCTGCCCTTGGTGAGCTCCACG







TATGACCTCATGTCCTCAGCCTATCTCAGTACAAAGGACCAGTAT







CCCTACCTGAAGTCTGTGTGTGAGATGGCAGAGAACGGTGTGAAG







ACCATCACCTCCGTGGCCATGACCAGTGCTCTGCCCATCATCCAG







AAGCTAGAGCCGCAAAGTGAGTTCTGATTTTTCAAGATGCAAGTC







AAGGGACTGGTCAGTGGCGAGCCCCTGAGCTCACAGCAGTAACAT







CTGGAACTCCTCAGCCCTTCCTGGGGCATACACTGCCTTTACCTA







GAAACTTTCAGGTGACCCTCATTACCGTAAGAGGACTGTTTCTTT







TCTTTTCTTTCCTTTTTTTTTTTTTTTGAGATGGAGTCTCACTCT







ATCACTCAGGCTGGAGTTCAGTGGCGCAGTCTTGGCTCACTGCAA







CCTCTGCCTGCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCCG







AGTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene PLIN2. In some embodiments, the sequences from gene PLIN2 comprise or consist of:











(SEQ ID NO: 1164)



CTAATTTTTGTATTTTTAGAAGAGACGGGGTTTCACCACGTTGGT







CAGGCTGGTCTTGAACTCCTGACATCAAGCAGTCCACCCCCCTCG







GCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGTCCGG







CTTGGCTAGCATTTTTGTTAACAGATACTCGGAGAAACAAGAGAA







GTATGTCAACAACTTTACTTCTCCCTCCTGAGTATTGCAAGTTTA







CACATAAGCTCTGAAACTTTGCCTCAAAAGAGGGAGTGGGGTTGT







GTGGGTGTGGGTCTTTCAGGAGGGGGACCTGGAGAGCTGGAGAGA







GGAAAGAAGTGCAGAAGACTACAGGGAGCTCACCCTGCATTTTTC







TCTACTTGTAATCAGCATTTGCTTGGGTTGGGGAATGAATTTGGG







AGAGTCTTATCCCGGTGTTCTGAAATAAGTCTCTTTTCTGCAGAT







TTCTTTCTCCAGGAAGAAAAATGGCATCCGTTGCAGTTGATCCAC







AACC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene SURF4. In some embodiments, the sequences from gene SURF4 comprise or consist of:











(SEQ ID NO: 1165)



GTGGCATTCTGAGCAACAGGGAAGCCTTTGAAACAGGCGGTTGGG







TAACTGTTGAGAGCATTCGGGAACTGGGTGGACAGGATCCGATCC







TACAAAGTGAAGGGACACAACTCCCTTCCCTCTTTCTTGATTAAG







CTTGACAGAACAAAAAAACAAAAACAAAAGGTGGTGGTGGTTACG







CAGATTTATGCCTTTTACTTCTAAAAGAATCCCGTTATGTCGACA







AATTAACAGCCTTATCCCCGCAGGGAGAGCCTTGTGTGGCCCACT







TGCCGTTTTTTCCTCGGTTTACTTAATGTGTTCGTGTGGCTTTGC







TAGAGTTTTAAGTAGTGATGGTTAAATTTTTAAATTTTTCTGGAG







GTGGTAGTGAGTCATCAAACTCTGAGGCCTAGACTTATCGGAAGG







CTTGTATATGTGTGGTTTAAAAAAAAAAGAAAAGAGTAAGTGACC







GTTTTCTGATCAGCTATTGAGCTCTTCCGTTCCTCCTGGGGGGTG







ATCT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene SURF4. In some embodiments, the sequences from gene SURF4 comprise or consist of:











(SEQ ID NO: 1166)



CCCGACCCAGGCCGCCCGGGCCGCTCCGGAGTCGGCCTCCCAGCG







CAGGGCGCCAGGCCCGGGCCGCCCCCTGACTCCTTGTTCCCACAG







AGGGACCCCCCCGCCCGGGCCCTCTGTCCCGGCTGGACAGCCCCA







AACCCGGCCTCCGGGCCCAGTCTCCCCTGCCCGCTGGGCCGCTCC







CGGAGCCCGGGCCTCGGCGCCCTGCCTGGCTAGCCGCCCCCACCC







TCCAGCCAGCCCGGGGCGCGGGCTGTGGGGGTTTCTTCCCGGGCC







GGCGGCGCGAGTCCTGGCGTTGGGTCGGGTCTGAGGCTCGCGGGG







AGCGCCTCGGCGTTTGGAGCTGCTGGGGGGTCCCCGCACGTTTTT







CCTCTCACTGGGGCTCCCGGAGGCGGGCTGGGAGCTCGGGGACGA







ACGGGGGCCGGGGGTCCTCCCAGAGGAAAGGGGGCGCGCTGGCGT







GCGCCCCAGCTACAGGAAGCCACAGCACCTTTTCCTTTTCTCTTT







CCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene DNASE1L1. In some embodiments, the sequences from gene DNASE1L1 comprise or consist of:











(SEQ ID NO: 1167)



CCCTATTCAGGCAGGAGCTGCTCTTCTGGGGTATCGCGATCCACT







TAAGGATGAGGCAGACTTGGTGACAAGCTGGTCTGAGCAGGTATG







GGAGCCCCCTGGGGAGACGGAAGAAGGGAGGAAGTTGCCTTCTGC







CTGGGGAGGGTTTGAGAGGGAGAGGGAAGCCTAGGGCTCCCACCA







AGGCTGATATTGACAGCCAGGGTTTGGGGCTGAAGCCAGGAACCG







TCACTCTCTCTGGTTCTTACTGGTAGCCCTCATGGGGGTCCCTGA







CGCCAGAGCCTCCAAGGCTGCATGTGCCAGCCCAGGGCTGCCCAC







ATACCCATGTATATCCCAGAATAGGCACCAGGGTAGGGAACCCAA







ACTAGCATGAGTGACAGAGCAGGTGGTCAGGGAGAAACAGACATC







AAACCCAGCCAGGAGAGAGACACCGCAACAGAGAGACAGAGAAGG







GAAACCAGAGACGAGAGGGAAAGTGAGACAGAGACGGACAGAGCC







TCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene DNASE1L1. In some embodiments, the sequences from gene DNASE1L1 comprise or consist of:











(SEQ ID NO: 1168)



CCTCCCTTGACTTCACGACTGTGGCCAGATCATGTGTGGACTGTC







CCTCTCTTTGGGTCTCCAGAGCGCTTGCATCAAACACCCCTAACT







CAGAAGTGTGCAGCCACACTGGGACTCAGAACCCAACAACAGGGA







CAGAAGACTCACGCCCTTGGGGTGCCCGGTCTCGTGGCATCAGGC







ATGACTTCCAGCTCCTGCGCCTTCCCCAGCAACTGCTGACTGGGG







ACCCAGACCGGGAGCTGAGCGACGGGCCTGGCGAGCGAAGCTCGG







GGTCTCACTCAGGCACCAGCCCCTCCTTGCCCCAGGCTTGAGTGA







CTCACAGGTCTGTCATGTCCCGCTGGCCCGGGAAGGCGGGGTCTG







GGTGGGAAGGTGGGCGGGCAGGTCAGCAGTTCCCAGGCCTGGTGC







TGGTCCTCCTTGTTGGCGGCGAGGCGGAGACGGGGCGGGCCTGCC







TGTTGGCAAGGGTGGGGCGTCCGGCCCAGCCAGGCCCTTCCTGGC







CGGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene IDI2. In some embodiments, the sequences from gene IDI2 comprise or consist of:











(SEQ ID NO: 1169)



GTACACACAGCATCCAGTCTCAGCTGTGTCCACGGGAGTCCAGGT







TGGCAGCGGGCAGACGGTGAACAAAGTCCAGGCTGGATCTACACC







ATTGACCTCATGAAGCACCATGAGGATGAAGGATGGGGCTGTGGA







TGGGGCTGTGAATGAGCTGTGAATGGGGCTGTGAATGGGGCTGTG







AATGTAGCTGTGGATGGGGCAGTGTGAGTCCCTATGTGTCCCAGG







CAGGGTTGAGACTGTGCAGGAATCTGTCGACCACACACCGAAGAG







GAAATTCAAGCCGGGCGTGGTGGCTCACGCCTGTAATCCCATCAC







TTTGGGAGGCCGAGGCGGGCGGATCACAAGGTCAAGAAATCAAGA







CCATCCTGGCCAACATGGTGAAACCCCGTCTCTATTAAAAGTACA







AAAATTAGCTGGGCGTAGTGGCACACGCCTGTAATCCCAGCTACT







TGGGAGTCAGAGGTTGCAGTGAGCCGGGATTGTGCCACTGCACTC







CAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene IDI2. In some embodiments, the sequences from gene IDI2 comprise or consist of:











(SEQ ID NO: 1170)



TCTCAGTAAGGAAACTTGGTCAAAGGTATTCCAGCAGTCTTCCTG







TGAAACCATGCTCACTGATCACCACCCTCAAAGTGCAGCCCAGCT







TCCTCAGCCCAGCTGGTGCAGCCACCCAGCCCACAGCCCCACCAC







CTCCTCCCGCCACCCCATGGCATCTCAGGTCCTCTGTGGGTACAA







AACTGAGTAGGAACCAGTCCCTCTCCTAAGACATACTGGGATAGT







GCCACGGGACATGTGTGTGCACATCTGCAGGAAGGGCCGCAGGAG







CGGTCAGGGCCAGCCCCGCCAGGTGGTCATGTGGAAGACCCTCAC







CAAGCCCACAGCCGTGGAGGCTGGAGGCGAGTGCTGCTCTGAGAG







CCCATGACAAACCCACTCCCCCCGCCAGGCTGTCTTCTGCTGATG







ACGGATGATGGTTTTACATGAAGCATCTGCATTTGTATTTAATAT







ATTCACTTTTCAGGAATAAAGAGGGATGTTCCCACATGCATTTTT







TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene AKR1C3. In some embodiments, the sequences from gene AKR1C3 comprise or consist of:











(SEQ ID NO: 1171)



ATGTTGCTTTAGTTTCTAAGCAACATAAATAGCTATTCTTAAGCA







TTGGGTTGAATGGATAGAAGAATTAGACTGTTAAAATGAGTTGTA







AACTCTACTGAAGATAATTCAGGTAACATCATAGTTATTACTTAA







TACTAATCTTTACATTTTAAGAATTTACTCCTATCATTCAGTAGA







TGTACAAACTATACATCCAACGTATAATAAAGTTTATAAGGATAG







GTCAGAGCCAAAGGAATCATGAGAAGGAAGAGAATATGTTTGCCA







GTGGTCATAAATTTTGAAGCAGTAGGAAAATATCTAAATACTAGA







TGGCACAAAGTAATAAGATTTGCTCAAGCATTCATTCAAAATCAC







CTCCATTCTTTAACCTCTGCAGCTTTGGTCCACTTTTCATCGACC







AGAGTTGGTCCGACCAGCCTTGGAAAACTCACTGAAGAAAGCTCA







ATTGGACTATGTTGACCTCTATCTTATTCATTCTCCAATGTCTCT







AAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene AKR1C3. In some embodiments, the sequences from gene AKR1C3 comprise or consist of:











(SEQ ID NO: 1172)



AAGACATGGCTACATTTAAGGATATGAAATAGTCTACAAAAATGT







ATCATTTGTCTATTCTTATCTCTAAACTCCAACACTTTTTTTTAT







TATTGTTGACCCAAACTTTTTCATTGGTGGCGATTATGGCTTTGG







AGCAGCTCAAGGCTCATGGTCATCACCATAAGACAACTCCTCTGG







GTCTCCCAACTTCTGCTGACCCTATGTGATTCACAGTCCTGCCCA







AAGACTATCAACTCTTCAGGTGGTCTGATAATCCTACCGTGTACA







AGAGTACAATCCATTATTTTTGGATATCAAGGTATTTCCAAATCA







AAGAGAGGTACTTCAATAGAGGAAATTATAAGGGCGGAATAAAGT







GACATAAGTCAACTGTGAAACTTGATTAAGACCTAGAAAATGGTC







CTTTTCCATGCATATAATATTTGTAAGAGATTAGAGGAAGCCTGT







CTCCTGAATACATTCCTTATACCTTCATATGTAAAACACTTAGCA







CATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene TIMM23. In some embodiments, the sequences from gene TIMM23 comprise or consist of:











(SEQ ID NO: 1173)



GTAAGTCTCTTGTAACCATCTGATGTAGTGATACTTGAATATTAA







GCTCTGTTGTATTGGTTTGATGAGTACAACCTTGAGATTACAGAT







GGTTAGCATAGTTATGTGCTTTTTTGTCTTTGTTTTTTAATCTTA







ATCAAAATAAAAGCAACTAAGGAATCAGATTACTGACTCTAAGCT







TTTAAAAAGGAGTGATTTTTTTAATCAGTGTCCCTCAGTCAGCTA







GTGCTATGCCAGCCTGACTTCTCAGCTGCACCTGTTACATTCTCC







AACCCTATGTGACTTGCTTTTCAAATTGTTGAGTTGCTAATGGGA







ATTTTGGATTGTTTGGGGGCATGCAGATCCATAGGAAATGGTAAT







GAAGAATAAGCAGCTCATGAGAGTGATGGCTCAAGCTGCCATCAT







TTAACTTGAACTTCTTACTGTCTTGACTTTATGTTTCCGAAATGA







TGAGCCAGATACATTTTAAATAAAATTTTTAGAATTAGAGTTAAT







TCCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene TIMM23. In some embodiments, the sequences from gene TIMM23 comprise or consist of:











(SEQ ID NO: 1174)



TTTCACTTTGATTTAGGTGTTACTGCTGCAGAAAGTTTTATTTTT







GTTTAAGTATAGGTTTGAGTTAATTTAAGGTTTATTTTCTGTGAA







AAACTACTTTGAAATGTTAAAAAGGCAACTTTACAAGATTTGTGT







CTTGAGGGACACTCAGCTTGGTTTTCATTATTATCCTTTTAGGAT







ACAGATGAGTTTATTTTACCTACCGGAGCTAATAAAACCCGGGGC







AGATTTGAGCTGGCCTTCTTTACGATTGGAGGATGTTGCATGACA







GGTGAGTGTTACATACTTTTTTCTCAAGAGTGCTCAGTTCAAGTA







GTTGAGGGTGCGTAAAATCAAAAGCAATTAGAATGTTGGTTTCAG







GGTTTTTTTTTTAATATTTACATTTGTCTTTTTCTATTAAATAAA







AATGAAAAAGAATCAGAAGTGTAGTTATGCAGATTTGAGTTTGTT







TTTTTAATATAAAGCTGTCTTTATCTGGGTGAATTGTTATGATTT







ACCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene MYPN. In some embodiments, the sequences from gene MYPN comprise or consist of:











(SEQ ID NO: 1175)



AGTAAAGGAAGCTTTGTTGTGTTTTAATGCAAACCACACAGTAAA







GCTTTAATTTAATCACCTTAAAATTAGGGGAACAAACAGGAAGTG







TTGTAATAATTGATTGTTTTTTCGTATTTACCTTAATAAATGCCT







TGACCATAGTCCCAGTTTGGTACCAGATAAAGATATTTATACATG







TAATATGCATCCTGTGTATAGATATCTTATGTATTCCATATGCTT







TATTCTTGAGAATTTCTTCACTCTTATTTTCATTTAGACTAAGGA







GAAATCAAGAAAGCCTATTTCCTTAGTCATCTTAGTCATTATCCA







CCCCCACCAGCCCTGCAGGCTAAGAACATCGTGCAGACTGAGTTA







TTAAAGTTGATCTGAAAGAGCAATCTGTTCTAAATGACTGTCCCT







GGATCTGCAACGGAACCCTGTAAATACCCTTCATTACTCTGGCGG







GGCAGTATCACATTTCCTTCATGGCACTTTTGTGTCTGACACAAG







TTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene MYPN. In some embodiments, the sequences from gene MYPN comprise or consist of:











(SEQ ID NO: 1176)



TAACCACAAATTTACTTCTCAGGATAATGTTTTAAAGTGGGTGTT







CTGTTTAGTTTTTAAAAATGTTTTTAGTTTTTAAAACTAAGTGGG







TTTTGAACTTTTAGTTTTTTTTTTTAATGTTTTTAGTTTTCCCAG







GCAAGCATTTTCAGTAGTTTAGATGAAAAAGAAATTTCTTAGTGA







TTTCATTGTCTTTTTCAATTATATTGTTGTCATGCCTTTTATATA







AGATCTTTTATAATCCACCACCCATCTATTGTTTTGGATAGTCAA







GAACTTGTCTTTGCTGTGAAAATTTTTCTCATTAAAAATGGCTAA







AAAATACATACTTTCTCTGTTTTTCAAAAATACGGCATAGAGGTT







TTGTTTAGTTTTTTCATCTGATACTGGATATGTCAAGATGAATCT







ATGAACTTCAAGCAAAACTTATTGAAGCTTTGGTATATGTGAGTA







ATTTGTTTTAGAGAACTGGAGCCTATAGCGATATAAAAAAATAGC







AAAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene TNNT3. In some embodiments, the sequences from gene TNNT3 comprise or consist of:











(SEQ ID NO: 1177)



CCACCCTCCAGCACCGCCAGAGGCCCCAGGGGAGGGCGGCTGTGC







CCACGGGGCTTGTGCTCTACCCAGGTGCAGCAGGTGTGGGGCGAC







CAGTCCAAGCCAGCCGGGCAAATCCGAGCTCCTTCTCGGGGGTTC







TGGGCAGGCCACCTGTTCCACCTGAGCTTCCACGAGCTCCCCTCC







GACAGAGGCAGGGCGATTACACTCCCTCCCAGAGCTGTCCCCAGG







GGGTCCAGGCATGAGGGACAGCCCTGACCTCACCCTTGGGGCTCT







CCCAGAGGGGCTGGCCCTGAGACGGGCTTCACGGCACAGGACCCA







GGTCCTTCCCATGCTGTGTTCCCAGAGCTGAATCAGGGACCCACT







GGCTTTGGATAGATGCGGCCACAGCGGGCCCCCAGGGGCTCCAGA







GCCTGGAGGGCCATCACCAGGCCAAGCTAGCCCACAGCCGGGCCT







CGATGCCCCAGCCGCCCATCCAGCCTTGTAACCATCTTGGTCTTT







CTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene TNNT3. In some embodiments, the sequences from gene TNNT3 comprise or consist of:











(SEQ ID NO: 1178)



TAAGTAGCCGGGTCCGCCCTGGGCCAGGCCCGTGGGTGTGCGTTG







CACGGTGAGGTGAACCTCTCGCATGGCAAAAACCTGGATCGCTCA







GGATGCTGGCGGCAGGGGGCCTGGAGCCTTCCTCCTGCCCCCGCT







CTCCCCTCGTGCCTGCAGCCGGGGGCTTCCCAGTGGCCGCTGCCC







TGTTGGCAAGCGAGGAAGGGCCCCCACCTTCCCAGGAGCTCCGCC







TCAAGCCAGCAGCGGCCACAGAGTGCCCCTGGGCACAGGCACGAG







GGCGGGAGCGGCTGGCCCAGGAGCAGCAGGGCCGTTCGGAGCTCT







GAGCTCAGCGGCTGAAGGGAGCTGGAGCTGAGAAGGAGGGGTCCG







CAGGCCTCACCTGCAACCCCTCCCCAGCCCCATCCGTGCTCCGTA







CCCCCCGCCCCACATCCTGCCGCAGGCTGAAGGTAAGTGGCCAGC







TACGGGCTGCTGTGTGTGGCAGTCCAGGCCAGTACAGGCTGGCGA







GAGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene ZBTB16. In some embodiments, the sequences from gene ZBTB16 comprise or consist of:











(SEQ ID NO: 1179)



GCTAGAAGGGGGAGCCCCGGCTGTCAGCCTGGGCGCAGCTGCCTC







CCCAGCCCTTCCTCTTCAGGGCACGGTCGGGGTGAGAGGTGGGGG







GCGTAGCGGTGTGCGGGGGCTGAGGGCGTGAGCAGAGGGGTCGGG







GATCCGGAGGCTTTGTACCGCCAGGGGCTGGCGGAGCAACAGAGC







CCGTGGGTGCTCTTATGTATGCGGACCGGTGCGCGGGCGCAAGAT







AAGGTTGTGGTTTATTTATTTGTGTGTTTATTCGCCGGCCGGCTG







GGAAGCTAGAATCGGAGGAGCTGACGAGTAGATCTGGGGGCGGAG







GGGAGCAGGACTGGGACTGCTTACGTTTTGTTTCTCTTTGAGAAA







ACGTGGTGGGCTTTTTCTTGATTGGACTTGATCCCCACCCCCCTT







TTGCAGGGGAGGGAGGGAAGCTCCAGAGGGTCTGCAGCGCTGCGG







GCCCTCCTCGGCTCTCGGCGGGACCGGCGGTGACACCGGAGCTCG







CCGT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene ZBTB16. In some embodiments, the sequences from gene ZBTB16 comprise or consist of:











(SEQ ID NO: 1180)



TGGGTGCCGGTCTCTGCACCTGATGCGTTCGGGATGCCTTTCCCA







CCCTGGCGCGCCCGCCGCTAGCTCGCACAGCGCCTCGCACACTCC







CGCACGCGCTTGAAATGCGCACGGTCCCGCCGGCCCGCGGAACCA







CCCGGACGCACGGAGCGCTCCGCACCGACTCGCTCGCCGCCTCCC







CGAGACGCTCGCACCGTGCTTGGGCCGGGCGCGCTGGCCGCTGGC







GCCGCTGGCCAGAGGCCTGGGACCCAGCCGGTCGCTCCCAGGGGG







TCACGGCCCTGGGTCGGAGAGGGAGGGCGGGCAGACCCCTTCTCG







CCTTTCCTCCCACAACTCGCTGCGGGGCTTTTGTGCTTCCCCTTC







GCCGCGGGGCGGGTCCGCCTCCCCTGCCGCTCTCGCCGCGGAGTC







CAGCCCGCCCGGACTGTCGCCGTTCCTCCCCGTCTCTTTCGCTTT







CCCTCGTCCCTAGCTCAGCTCTCCTTCTTTCAGGAGTCTAGCTCC







TCGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene STAC3. In some embodiments, the sequences from gene STAC3 comprise or consist of:











(SEQ ID NO: 1181)



GTGAGTCTGGGCCTCTGACCACTGGGTGTGGGGGGCTGGGAAGCC







ATTACAGCTGAAGAAGAGTGCTTAAATTGATCTCATTTGTGTGTG







ACTCATTTTTTGCTGGCCAGCACCTGCAGTCCCCAATACCAATAC







CCCCCTAATCCTGGGGTACATTCCTTGCCTGAAACTAGAAAACTG







TCTAGTTTGCTCTAGCGCCCCCCTACTCTAGAGAGGGTGACAAGT







CCTTTTAACATTGGGTTTTCTTCACTCCCTCCCTCAGCTACAGCG







GCTAAAGCAGTTACTCAGGAAGGGTTCTACAGGGACAAAGGAGAT







GGAACTTCCCCCAGAGCCCCAGGCCAATGGGGAGGCAGTGGGAGC







TGGGGGTGGGCCCATCTACTACATCTATGAGGAAGAGGAAGAGGA







AGAAGAGGAGGAGGAGGAGCCACCCCCAGAACCTCCTAAGCTGGT







CAACGATAAGCCCCACAAATTCAAAGATCACTTCTTCAAGAAGCC







AAAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene STAC3. In some embodiments, the sequences from gene STAC3 comprise or consist of:











(SEQ ID NO: 1182)



GATTACAGGCATGCACCACCACACCCGGCCATGTTAAGCTTTTTT







CTAGACCTCCATGAAATCTGTTAATAAAACCTGCCAAAGGATCTG







TATTTGGCCCAGTGCAGTGGCTCACGCCTGTCTCAACAATTTGGG







AGGTTGAAACAGGAAGATTGCTTGAGCTAGTTCGGGACCAGCCTA







GGCAACAAAGCAAGACCATGTCTCTACAAAAAATAAAAAAGTAGC







CAGGTAGTGTGCACCTTGTGTTCCAGCTACTCAGGAAGCTGAGGT







AGGGGGATCGCTTCAGGCTGGGAGGTCGAGGCTGCAGTGAGCCAT







GATCACGCCACTGCACTCCAGCCTGGGTGACAGAGTGAGACCCCA







TCTCAACAGTAACAACAGCAAAATCTCTCATCACATTTCTCCGAA







AGCCTCCCCTTTGAGGACCACCCTACTGCAAGGGACAGAAAGGGA







GAGAAGAATGAAAGGGATTAGATTTCATAGTGGGGTCCAACCTCT







TGCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene BEST3. In some embodiments, the sequences from gene BEST3 comprise or consist of:











(SEQ ID NO: 1183)



GTAAGTGCATTTTTAAAAAATCTCTAATATTTTCCAGCCTGAGCA







ACATAGTGAGACCTTGTCTCTATAAAAAAAAAAAAAGAAATAAAA







TAATTTAAAGTAGCCAACTGTGATGTCACCTGTAGTCCTAGCTAC







TTGGGGAGGCTGCAGTTGGAGGATTGCTTGAGCCCAGGGGTTCAA







GGCTGCAGTGAGCTGTGATTGTGCCATTGCACTCCAGCCTGGGTG







ACAAAGTGAAGCCCTGTCTCAAGAAAAATAATTAATTAATTCACG







AAAATTAAAATAAATATCTCCAATATCTAGGAAATGAACAGATAG







ATCACTGTTCTAATCTTTTTACCTTTTTTGGCATTTTTTTCTACC







TAGGCTTAGTATGTAAGTCAGTCTCTGTTCAGTGGTTTTAACACA







GCAATCACCCCTAAGTATAATGAGCTTTAAATAAATGAGTTGAGG







CCTGAAGTAAATCAGGTACAGTTCTATAATAATTTCATTAAGTCT







GAGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene BEST3. In some embodiments, the sequences from gene BEST3 comprise or consist of:











(SEQ ID NO: 1184)



TGAGCAGTATAGACTTCTTGTTATGTTTTTCCAAACAAAGAAATG







AACAAGAAACCAGGGCATATAAGAAGTGATGTCAAGTAAAAATGT







GTTTACAGGGTTAACAGAATGAAAAGCGGAATCTTCACCTTCCTG







TAATATTTGGGGTTAAGTAGGTGCTATCAGGTAAAACAACACCAC







AATTGTGAGCAAAGAATAAAAAGATGGCTCCCCTCCCTCCAGCTA







AGAGGCAACTCCACTCTAGAGTGTGTGGTTTGATCCCGGAGTGTG







GGAGGCTTCAAGCCCCACTTATCCTCATCGCCAAGTGCTCTCGAG







TAGTAAACAACAGATACAACTCTCTGTGGCAGCTGTGGTCATGGC







AAGTATGAACTACAGACATTTGTATATTCACTCATTTAGACTGTG







TCCTAGCCCAATCTAACTCTCATCTGCCCAAAAAAACTTCTCCTT







TTTATTTAATTTACATCTGAGTTATTCTAACAATATAACAATGAT







TTTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene MYBPC1. In some embodiments, the sequences from gene MYBPC1 comprise or consist of:











(SEQ ID NO: 1185)



ATCTTGACTTTTGTTTCTTAAATACTGAGAGCAATTAATCATGAG







GTTTTCTGGGTAGAAATGAGTTCTCAGATAGTACATATATAGGCT







TTTATGTCTGTGTCCAAACGAATGTGATATATGAATAAGGTCTGC







CATTCACTTTAGTTACAAAATCAGGACTTGAAAACCACTTCCATT







ATTGATTTCATCAAGGTTTGATTTTTAGCATTCATTTTTTCCTAA







CCTACAGTCACATAAAAAATTTGCAGTGTTCCATTTTTAAATCTG







TGCTTCCATTTGGATTACCCTACTCTGTCAACTTTTTATTTATTA







TTTTTTCTTTCTTTGCCTGAAAAACTCAGGTTTCACTTTTCAGGT







ACCAAGTTTTCTAAGGCTTGCTCAGCAGTTGAAAGTGTATTGTGT







GGTTTCTCATCTTCTTTTTCAGATTTCTCTTTTCTCAGGTTACTT







GGGATGAAGTCTTTTCTCCTTGACTTTTTACTCCTATTTCTTGTT







TCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene MYBPC1. In some embodiments, the sequences from gene MYBPC1 comprise or consist of:











(SEQ ID NO: 1186)



TTGGGATAATTTCTACCCTTTTCCCTGCTTTTAAATATATTATTC







ATTGACGGTAGAGGAAAAGAAAAGGCAGTGAGCCTCCTTGCTGAG







TCAGTGCACAGAGCAGGGGCAACAGTTTTCGCCTGTGCTGGCACC







AAGAAGGAAATGTTGGCTTTAATGACAATTTTTTGTTTTACGTGA







GAAACTTCTTAGAAAGAGGCATTCTTTGAGAAATAAATAAGATTG







GTAAATCTTGGGTATAAATGTTAGGGGTCAACATATCTGTCTTCC







ATTTCTCACTATATATCCACCCTTTTCCCTGTTCTTTAAATTTAG







ATATTTGGATTTTAAAATTTTTCTTTTCTTTTTTGAGACTGAGTC







TCTCTCTATTGCCCAGGCTGGAGTGCAGTGGTGCTATCTCGGCTC







ACTGCAACCTGTGCCTCCCAGGTTCAAGTGATTCTCGTGCCTCAG







CCTCCTGAGTAGCTGGGATTACAGGTGTGTGCCACCAAGCTGGGC







TAAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene COMMD6. In some embodiments, the sequences from gene COMMD6 comprise or consist of:











(SEQ ID NO: 1187)



GTAGCCGTTGCCATTGTAATGAAAGCTCCTCTTCCCCACCCTGCA







CTAGCCCGAAGAATGCAGATCAAGGATTGTTGGGCTTTGATTAGG







CGACAGTTATTCGAAGCCAGGAACACGGTATTTTAAAGTGTTGGA







TAAAGAAGTTAGGAAAATTTGCAGAGATAGCACCGCAAACATTTT







CTTAGTCTCTAAGTTATTTTTGAAGATGGAGTATAGCATTTTAGC







CTTTTAGAGTATGCATTTATAATGCTAGGAATATGAATTTGACTT







TTAAAGTATATCTTAGCCCCATTTCTGTTTCAAGTGCTGCTGTGC







TGTTGTAGCCAATAAACTATTTGTAGGCAAATAATTTATAACACA







CTTCCCAGAATAGGAGTACTAAAACTAGAGTATTTTTAGGCCAGG







AGTTACCCAAGTCCATACAAAATGTAAGAAAGGGATGAGGTAAGA







AATAATTAGAATACAGGGGCTGAAAGAGGTGTCCATTGACTGCTG







TCTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene COMMD6. In some embodiments, the sequences from gene COMMD6 comprise or consist of:











(SEQ ID NO: 1188)



GGTTGGGATTGAGGAGCCGCGCAGCGGGATGCTGTGCCCCGCCCC







GCTCCAGTCTGGAAGAGCCCACGTGTCACTGTTAGATGGGGGAAG







CGGCATCCTAAATTTAATTAGCGCTTCTCTTCTCAAAGTATCTCC







CAAATCTTTGGCCTTTTTCCATAAAATAATTTTCTTTTAGCTCAA







TGTCTCCTAAGATATATGTTGAATTAAGAAAAAATATGAGTCACA







TAAAGATGCTTTTTTCAGCTTCCCCTGCTACGTCCGCCCCATGCG







CCAGGCCGCTAGGGGCAGGACGTGGAAGCGGAAGCGCTGGTCTGG







GGGCGTTCTCTCGAGTCCGGGCCGCAAGTCCCAGACGCTGCCCAT







GGAGGCGTCCAGCGAGCCGCCGCTGGATGCTAAGTCCGATGTGAG







TTCCAACCTGGAAGGAGAGTGGGCAGCGAGGCTCTGGAGCAAGAA







AGATGTTTCTCTAAATTGTGTTTCTGCTTCCGTTTCAGGTCACCA







ACCA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene SLC7A8. In some embodiments, the sequences from gene SLC7A8 comprise or consist of:











(SEQ ID NO: 1189)



ATCAACCTGCTGTTCCCCATCATCTACTTGCTGTTCTGGGCCTTC







CTGCTGGTCTTCAGCCTGTGGTCAGAGCCGGTGGTGTGTGGCATT







GGCCTGGCCATCATGCTGACAGGAGTGCCTGTCTATTTCCTGGGT







GTTTACTGGCAACACAAGCCCAAGTGTTTCAGTGACTTCATTGGT







GAGTTGTTGGAGGCTCTGGCTGGCTTCAGGGCCTCCTGGGCTGGA







ATGCAGTGGTCAGTGATGGGGTTGGGGAGCCACCAGGGCCAGGAG







ATCTAGGCTCACCTTCTCCTCAGTCCACAGAGCCCTGGAAGAGTC







CAGAGCAGGGGAAGCAGATGCCTTTTCCCAGAGACCTCTGAGGGT







AGAGAACAGCTGGCATTTCCACACAGACCTTAGACCCGCAAGAAT







AGGCAACCTGGGCTGGGCACAGTGGCTCACGCCTGTAATCCCAGC







ACTTTGGGAGGCTGAGACAGAGGATCACTTGAGCCCAGGAGGTTG







AGAC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene SLC7A8. In some embodiments, the sequences from gene SLC7A8 comprise or consist of:











(SEQ ID NO: 1190)



AAAATCTGCTAGAGGGAAGAGGAGGTAGAATAACCCCATGTCCCC







ACCACACACATCCCTTCTGAGCGAGGAAGACAAGGAAGAGAAAGG







CAGCCGAATCAGGACTGCCCCTGGAGCCCTGAAGAGGATACTCTC







AGGAACTCCCCACATGTGGCCCATAAGGAGCTGACATTTGCCATA







TTGTGCCCACATAAGGGCCCTGCACAGAAGTGGGCAAGGACCAGG







GAGCCTGAGTGACTCGAATCGTCTCCTGCCTGCACCTTGTGCATT







GCCACACCTCCCCTCCACCCTAGGACCTCAGCAGACACACCCATG







TGCCAGCAAGCTTCTCCATCCCTGTGGGCTTCTCTGGCTGCCACG







GGCACATGGCATGCATGATGAGGCCTTGTCTAACCACACCCAAGA







GCGAGGACATGGGCAAGGAGGATCGTTTCCTCCCTAACTCCTCCC







CATCCTCTTCCTGTCTGACCGACCATGTTCATCAACATTTGTTTA







TATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene MYH7. In some embodiments, the sequences from gene MYH7 comprise or consist of:











(SEQ ID NO: 1191)



TCCTGACTCCAGACTCTTCTTCACTGCCCTGAGGACCCGGGGATG







GGGTCTGGACTAGGGGAGGTGGAAATGCAGGGGAGTGGGGGCAAA







TTCAAAGGCCTGGCCTAGGAGGCCTTCGTGGAGCAGCACTGAAAA







CCCCTTTCACTTCCCCCAAACCAGCCAAATGCAGCAGAGGCGACA







CCTCTCCTCCCAGCCTTCTCCCTGGTCCAGCCAGGATTTAAAGGA







GATTTAGGGAGACCCCTAAAGTTCACCTAGAGCACCCTGAGCCCA







TCTACTGTGGACCTGCACCCCAGCTTCCGTCAACCCTGCCCCACC







CACCACCATGGATACTGCCCAGGGCTGGGCAAAGGGCAGCGGTTC







GGGGTGTGGGTGCTGGCCAGTGCCCTTTCTGAGCCCGCAGAAGTG







TGAACCACTGGGCCAGGTGAGGCAATGGCTGCCTAAGTGCCAGTC







CCAGGCGAGTCTCTGAGAGTACACAAGGATTCACAGCTGTAGCCT







GCAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene MYH7. In some embodiments, the sequences from gene MYH7 comprise or consist of:









(SEQ ID NO: 1192)


CTCCTGACCTTGTGATCCACTCGCCTCAGCCTCCCAAAGTACGGGATTAC





AGGCGTGAGCCACCACTCCCAGCTCAGCTCTAAGTTTCAGTGGTTCTGGG





AATTTCAGCCTTAGGGAGGGGTGCCACCCTAGAAGACCCTAATTTATATA





CAACCAGTATATCCCAGTTTCCCCCCAACTGCTAGTTTCCAGTTCCCTTC





CACCTAATTTTGTCCTGCAATCTTGTAACTCAAACCTAAAATGGACATCC





CAGAGAACCCGGGCCTGGCTTTGTTCTCGCCCAGGCAACTTCCCTCTGAC





CAACCCCAGAACCCTACCCTCCAGAACTTGCCCCCCGAACATCGATCCCT





GGAAACACATGTGCTGCTGCCACAGGGGCTTCCACCTGCCCACAAAGCAG





GCCCCCCACCATCAGACCCCTCTCACCTTTGTTCCCATGCCCTGTCCCTG





CCCAATACCATCTCTCCAAGGACTGATTGGACTTTGTTTCCTTTCAAAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene CCPG1. In some embodiments, the sequences from gene CCPG1 comprise or consist of:









(SEQ ID NO: 1193)


ACGGCATGCACTGCGGCGCCCAGCCGGTCACCGCCTGCCGGCTGCAGACG





CCTGCGAGCAGGTTGTTTTTATAAGAGGCGTCATTGGCGCCCGAGCTGTG





ACCGCCGCCACTGGGGCAGCCAGCACAATCGGGCGGAGGTGGCGCTGCCC





CTTCAGGTACTGTACTAGGCGGGCTTAGACCCCGGGAGGGGGTTTGTGCC





GACTGTCTGAGTCCCTGGCCGACACGCTCGGCTCAGTAGAGGAGCAGTGG





CGGGGTCCGCCTCCTAGGCTGGGGCTGCTCGGGCGGGGACTTGACGCCTG





GTGTCAGCGGCCGCACGGGCGACCTCCTCGGGCCGTGCGCGAGTGCACAG





CTCCGGAGGCCCGAGCCGACCCTGGGGCGTCCGGTCCGGTGGTCTTGCAG





CCTCCAAACCCCGAGTGCTATACCGAACTGCGCGCCAAGGGTGGGAGAGC





TGACGGCCTGGGCCACCCTTCTTCCTTCACTGGGCAGGCTTTGAGGTGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene CCPG1. In some embodiments, the sequences from gene CCPG1 comprise or consist of:









(SEQ ID NO: 1194)


CAGTAGGGTATGAATGCATACAATCTCGATGGTCTTTACACCTTCAGAGG





CTATTCGGACTTAAGGAAACGTGCATTTACAAGTGTAAATGTAAAAGCTC





AGTAGTTGCCTTGTAAAGTGCTTTTCGGGGTTGTGGGGTTGGGGGTGGTA





GGAGTAGGTGGTGCCTCAAATTATACTAGCGAATACCAACATTCCCAAAC





ATCTAGCCCTCTGCCTAAACTCACCCGGTTGCGGTCTGTCATTTTCGCTC





TTCTTTGTTAATTTTTTCGGCAAAGTCAGTTTTCATGCAGCAGATGGCAA





GAAAGCCGGTGAACGCCTAGGGACGTGGCCTTTTAACCGCACCCAATTAA





AGCAGCAAACAGAAGATGAATAGCTGGGTGGATCAGTATGTGTAAACCTA





TCCGTTTAATCCGGGGAGCTGTTTTCCCGGGTGAAACCACGAATTCTTTC





TGGAGGTTGCAACTCGTAACCATAGCAACTGGAAGTGGTCGCCGGCTCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene NOMO1. In some embodiments, the sequences from gene NOMO1 comprise or consist of:









(SEQ ID NO: 1195)


GATTTCATAAGGACCTGTGTTCAGGTCGTGGTGGAAAGACAGGAGGGGGC





ACGTGAGACCTTGCAGAGGTGGAATCCACAGGACTGATCGATGCTGGAGG





AGCGGGGCGGGGCATCCAAAGTGATGCCGGGGTGTGCATGGGAGACTGGA





GGAAGACGTGGTTCACCAAAATGGGGGCATCTGGAGGGGAGGAGATGGCA





CCGGGGTAAGGGTGGGGGGCTTCTAGAAGCTTGAGTCAGTGACCTTTTAA





CGAGGGAAGTAAGTCTCTAGACTTTTCTGTTGTTTGACACTCAGCAGTGT





GTTGGGAACACAACAGAGAAGACAGATGTGGGTAAAGTGAGAGGGAGCCT





GGCTGGCACACAGGACCGGGGCACTGAGTGTTGGGAGGTGGGCGGCAGGA





GCAGACATGGTAGGTGGTGGCCGGCGCAGCAGAAGGAGTCTTTGTGGCTC





TGGCACAGGAGCACCTTCGAGCACCTTCTTCTTGTTCTTTGTTTTCTAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene NOMO1. In some embodiments, the sequences from gene NOMO1 comprise or consist of:









(SEQ ID NO: 1196)


TGGGCTGACACAGCAGCCCCAGGCTGATGGCCAGCGCTCTTTTTGGATCA





CAGAGAGAAATGGAGAGGAACTGATGACTATATGAGAACATGGCAGCTTT





TGGTCCAAGTATTCTGGTGTACATGTAAGGATACACTGTTGTTTTTGGCT





TATCTTCTGTTTGTGTGTTTTTGTTTTACAGTTAAAACAAAAGCTGATGG





CTCATTCCGCCTTGAGAACATAACCACAGGGACATACACCATCCATGCTC





AGAAAGAGCACCTCTACTTTGAAACGGTCACCATCAAAATTGCACCGAAC





ACACCTCAGCTGGCTGACATTATTGCAACAGGGTAAGCTTATCGTGTGGA





TTTGGAAGCGCCAGTAAATATGCTGGCAGCCAGTGTAGAACCGAATGACC





TGTGATCTGTGTGTCTTTGCCGAGCTTAAGAACTTAAGAAAGATTAGTAC





TTTTTTTTTTTTTTTTTTGAGAGACAGAGTCTTGCCCTGTTGCCCAGGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene NOMO2. In some embodiments, the sequences from gene NOMO2 comprise or consist of:









(SEQ ID NO: 1197)


GTGAGACTTGGAATGTGTTTTCTTTGGGGACTTTTTTTTCATCCTGTGTC





CAGAAGTATCTTGTGGTGGCCCCAAGACTGCTAGGAGTGGGTTGGGCGAG





AGGGCTGGGGGTGGGGCTCTGGAGCCCTCCCAGTTCTTACCTTTAATTGG





TAGAGTTTGCCTTTTTTCTAGCCAACAGGTCAGATCAAGGATCTGTGGCA





CAATGGAGTTGGAAAACCATGGGTTTGGTAGGGCTTTTCCCTAGCAGTAT





GTGGCCATGGCTTACGCCAGGGCTCAAGCCAAACGCCTATGGAGCCAGAA





GAGCAGTATGAACGGGGGAGAGGAGGCTGTGCGAAATGATAGGGAGTGGT





GAGGGCTGTGGAGTGGGCACGGCCTGTGGAAAGGGACAGCCTGTCACTTG





GTTCCAGCTTATTGTAGCCATGGGGGAAGACAGAGTCAGGGTTGCCAGAT





CCTTGAAATTTTAAAAAGAGAAGTCAGAAATTCCCACTTCCCAGAAATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene NOMO2. In some embodiments, the sequences from gene NOMO2 comprise or consist of:









(SEQ ID NO: 1198)


AAAAAAGATACACATCTATAGATAGAGAGATGCATACATATATACATGTA





TACACACAGATACACACATATGTGTATTTTTTGTAGACTTATACAGAATC





ACTTTAGAGGAAGAGTAATTGGAAAGATTGGGTTTAGAAAAATGGACCAT





TTTCTACTGAATATATGGAATGAAAGAGGAAGTGAGTTATTTCCATGTTA





TTGAAAGCTCCATTATTTCAAATAAAGCCTAGAAAGAATAAAATAAAGCT





TTAGCCTTCTTAAATTACAATTTTCCAGAGCATCCCTCAAAATGTATGAG





AAAAGAAATTGATGGCAGCTTATCCCTCCGGCAGGAAGGTGTGGTTTGAA





AGTGGGCCGGCCACACCGAGTTGCCTGGGTCATTGAGGCACAGTTCTCCC





CTGAACTATTTTGCCCCATAATTGTTGGTAAACGGGAAACACTTGGGTCC





TTCAGATAACCATGAGAATATCTCATTCGTGCCTGTTCTTGCCATACTA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene ATP2A1. In some embodiments, the sequences from gene ATP2A1 comprise or consist of:









(SEQ ID NO: 1199)


CTCCCAAAGTGCTGGGGTTACAGGTGTGAGCCACCACGCCTGGCCCAAAG





ACCCCTTTTCCAAGTAAGGTAACATTTACAGGCTCCAGGGATTAGGATCT





GGTATCTTTGCGGACCATTATTGTTTGTTTGTTTAATAGAGACAGAGTCT





CACTCTTGTTGCCCAGGCTGGAGTGTGGTAGCACAATCATAACTCACTGT





AATCTCGAACTCCTGGATTCAAACAATCCTCTTGTCCCAGCCTCCTGGGT





AACTGGTACCACAGGCAATCACCACCACACCCAGCTAATTTTTAAATATT





TTCAGAGGAGTCTCACTGTGTTTTCCAGGCTGGTCTCAAACTCCTGGCCT





CAAGTGATCCTCCCGCCTCAGCCTCCCAAAGTGCTAGGATTATAGGTGTG





CACCAACGTGCCCAGCTGGGGGCTACTATTTAGCCCCTTGCAGGTTCCCT





CACACCCTCCCCTTGCAGGTTCCCTCACACCCTCCCTCCCTCCCCACAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene ATP2A1. In some embodiments, the sequences from gene ATP2A1 comprise or consist of:









(SEQ ID NO: 1200)


TCAGGAGCAGTGTGGGCAGCGCGCTCAGTCAGAAGGCTGCCTGTGGGGGT





TAAATGGGCCCTCCAAAGATAGAGGTCTCCCTTCATCACTGCTGGCTTCT





CATCTGGGCCTGCAAAATGCTCAAAGGGCAGTAGAACGCCATCCTGTCTG





CCATGAACGGGATCAGAGAGGACCCTTGTGCCCCAGCCAGACAGCTCACT





CTGACCACCATCCACGCACCAGGCACTGCCACACATCGTCTCTCATCCCT





CACAATAGCCCATTCAGAGGATGGTCTCATTATCTTCATTTTTTTAATTA





AAAAAAATTATTATTATTTTGAGACAGAGTCTCACTCTGTCGCCCAGGCT





GGAGTGCAGTGGTGTGATCTTGGCTCACTGCAACCTCTGCCTCCCGGGTT





CAAGCGATTCATCTGCCTCAGCCTCCCTAGTAGCTGGGACTACAGGTGCC





TGCCACCACGCCTGGCTAATTTTTTTGCATTTGTAATAGAGACAGGGTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene ALDOA. In some embodiments, the sequences from gene ALDOA comprise or consist of:









(SEQ ID NO: 1201)


GGACTCTTGGCCGGCTGGACGGCAGCTCCTCTGGAGGGCCAGAAAAGAGA





GGGGCTAGTGCTCGGGCAGGTGCCCTGGCTTCCCTTCCCCTCCACACGTC





AACGATTCTATTTGAAGTTGGGCAGGGGGGTGGCGCTGCTCACCACACAC





AAGTGTTATAGGAGGAGTCTGGCCCTTGAGTACCGGGTACGCAGGGGTGC





CTCAACCACACTCCGTCCACGGACTCTCCGTTATTTTAGGAGGTGAGTGT





AGTGCCAGTATCTACTCTCCTTCTTAAAAAAAACCAGGGCTCCAGAGAAT





CAGAACAGCCACCATCACCGCAGGGAGTCAAGGGAGGAGGGAGATTAGAG





AAGGAGCCAGGGAGGGTGGCAGGGAGGCCACGTGATCCGAGTCCCCTCAC





CCCTTTCCTTCCCACAGGTCCCTGGCCAAAGATTTATTTCTCTTGACAAC





CAAGGGCCTCCGTCTGGATTTCCAAGGAAGAATTTCCTCTGAAGCACCG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene ALDOA. In some embodiments, the sequences from gene ALDOA comprise or consist of:









TGGGCCCCTTGCAGGACCGGGCCGGGGTGGGGATGGGGTTGGGGTTGGGC





CAACAGGGTCCAGATGGGGTCCAGGTGAGGAGGGGAGATTTGGACGATAG





GAGCAGGGGGCTCAGCATCTGGGAGGCAGATCAGTTCGGGGACGGATTTT





CTTTTGGAGAAGGAAGTCAGGCTCAAGGAAGACGTTTGGCAGGAACTGTG





ACCCCGCATGCCAGAGGCCGAGCAGCGGCCGGTGCATAGCCGCGCATTCT





GGTTTTCTGTGGCGCAGAGGACTACCAGCCTGGCTGCGGCGGCCCGGCGG





AGAGCGCGCACGCATGCGCGACCCAGCCCGGCCTGCCAGCCTGGAACTCG





GATGGGGAGGTCTCGCCTCCGCGCGCCGCTAGTTCCGCCGCCTTCT













(SEQ ID NO: 1202)


CGCGCTCCCTGCGTGTCCTCTCGATGCCCTTTCCTCCGCCTCCCTTACGC





CTGGGCCAGTGACAGGGTGTCGGCTCCGCGCCGATTCAGCCCGCGGGCGA





GGC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene CHRNB1. In some embodiments, the sequences from gene CHRNB1 comprise or consist of:









(SEQ ID NO: 1203)


TCAGCTGGGTTTTAGGTACCCAAAAAGGGCCTGTCTAGATTTTTTCAGAA





AAACGTGGAGTGCTAGGGGCAGCCTGGAAAAGATGGGGAACCTGCTAGTG





AACTAGGAGGGAGACTTCCATAGCCTCAGACTTGGATAGGGTAGGCTGAG





GGGGCCCTAAGGGAGGGACTAAGGCTCCAAGGCAGGTCACTTTTCCTTAG





GCTGTTCTACTTCTGGCTTGTTGCAAGAGGAGTAGATGCCCCCTCACCCA





CACAAACCCCACTCAGTCTCCACCCAACTCCTGGCACTGCTCCCAGGGGA





TCGGGTCTCCACTCCAGCTTTCTCAATTAAAGACGATTTATACAACTGAA





GTGCTGTCTGTCATCTGTCGGTGGCGGGCTTGGCAGTTGCTCGAGGCAGG





ATCAAGTCCCGGGGGCGGGGCGGGTGGGCTGGCGGCTGTCCTCCAGCAAC





AGGTGCACATTCCCGGGCTCCTCGTCACTTCCCCTGTGCTGGCGGTCCC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene CHRNB1. In some embodiments, the sequences from gene CHRNB1 comprise or consist of:









(SEQ ID NO: 1204)


TAAGTGTAGGCCCCGAAGGGGCAGTGACGGGGCCAGCGGTCGTGGCCAGG





CACCAGGGCTGCACTTATTCTCTCCTCCCCCAGGCGTCCGCGGCTCGGAG





GCGGAGGGTCGACTCCGGGAGAAACTTTTCTCTGGCTATGATAGCTCCGT





GCGGCCAGCGCGGGAGGTGGGAGACCGTGTCAGGGTCAGCGTTGGTCTCA





TCCTGGCGCAACTCATCAGCCTGGTGAGGGCGCGCGGGGGGTGGAGGTCA





GGCCAGCCGACCGGCCGGGGGCGTGGCTTTAGGCAAGGCCGGACCAGGGA





CAGGCTGGGGGCGGGGCCTGGGACGAGACCAGGCTGAGATGGACCAGCCT





TTGGTGAAGATTGGATCGAAATCAGACCAATGGACAAGCTCTGGCCGTGG





GTGGTGGACGGGCCTGGAGTAGAACTGGGTAGGGTGAAGGACGGACCTGT





GATCGGACCTTAAAGTGGGGCTGGGGACGAGGCAGGGGCTGGGGGACGG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene DHRS7C. In some embodiments, the sequences from gene DHRS7C comprise or consist of:









(SEQ ID NO: 1205)


GTAAGCTGGCTTTGCTCCCACCTGTGCCCATTTCTGCCCCCAGGGACTGG





CAGGATGGCAGGGTGGCAGGGCGGCAGGGCGCTGTGTGGGCAAACCTGCT





CGGTGGGAGGGGATCGATCCTGTGTTCTCTCCCAGCTTCTATCATTAACC





AGCAAGTTTCCCATCTTTTGGAGGCTGCAGATTCCTGATAAATGAGGGTG





GTGGAATATCTAGGGTTGCAAACTCACAGCCTAAGGGCTAAAACTGGTCT





GCAGATGTGATTTGTTTTGGCTTCATGGGGTTTCCAGAAATTTTGAAAAT





TCAAAGCCTTTAGCTGGGGCAATGTACTGGTTGTGAGCCCAGACTCAGGA





GCTGGGTTCAGTTCCTGGCTCCAGCAATGACTAGCTGGTTACTTCACTTC





TCTGCCTTAAACTTCTCATCTGTAAGGCGAGGATAACAGCAGTCTCTACC





TCATGCTGCCATTGTGAGGAATAAACGAGTTCATAGATGGATAGCACTT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene DHRS7C. In some embodiments, the sequences from gene DHRS7C comprise or consist of:









(SEQ ID NO: 1206)


AGGATGTTTGTGCTGAAAATCCCAGGTTTGGATCCCTCGTGCTCAGTCC





CAAACTGCAGAGGAAACCTCTAATACTCAGGCAAGCCAGGAGAGCTTTG





GCAAGAGCCCTGAAATCAGACCCGGCCAGCATGTGTTCCTGAATATCAG





AATAGACACTATATTTACCCTGACAGCCTTTTAGTGCAACTGAAAGCTA





TAAATAACCATTAGGGCCCAAGCAAGTGGCCTTAACACATGGATTTTCT





TCCAAAAATGCAGACCCATTTTAATTAAGTTTGTAATTAACCACTGGGG





AGGGCAGGCCCCCTGGATTCGGTCTGCTTTCGGAGACACTGTGAGTAAC





TTCCTATTTGTTGAACATTTGGGGATTAGCACGCCCACTGGGTGTTCAG





CTTGGAGGCTTGCACAGAGCTGAGCTCCCTGCAGCCTTGGGCCTCCCCC





TGCCCTGGGAGTCCTGATCAGCGTCTCTTTGCAAAGCCAATCCCCTTTT





ACTCCGTTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene NPEPPS. In some embodiments, the sequences from gene NPEPPS comprise or consist of:









(SEQ ID NO: 1207)


GTGCTGGGATTACAGGCGTGAGCCACCACGCCTGGCCACAACAAAGAAT





TTTACCAGAAGTGGGTATGATTTATATCTTAGTTTGAATTGCTACTTAC





CTTTGGGGGAACAGACCTTTCCTTGATGTATCTTTAACACTGAAATCTT





GGCAAGTTGCCAAGTCTCAAATGTTCATGAGTAAGACTGCAATATCACA





ATATCATAGTCACACAGGAATCTGATAGAAAAGCATCACATTTATAAAG





CCTTCTTTCCTGTACATCACTGACGTTTTGTGAATTTTAAAGAATTTGT





AATTATTTTTAAGGAGCATATTTAATGTAGATAATGTAGCCTAGAATAG





GCTCATTTGAAATGAAACTCTTGCTAATAGGAACTTAATTCACCAAATT





AAGAATATTTTGCTTTTGTAGAGATTTTGCTCTTGAAAATGTTGCAGTC





TTGATTTCGTCTTGTCAGTCCAGTCAGAATTGTAAAGTAATTTTTTTTC





TCATTCCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene NPEPPS. In some embodiments, the sequences from gene NPEPPS comprise or consist of:









(SEQ ID NO: 1208)


TAAGAGACATAGCTTTTGTAAAATCTCGTGATGAATATAGTGACATCTG





ACTTCCTCCAGAGAAATTTATTGTATGCCATTTTTCCCCTCATTGTTAT





TAGCAGATTAAGTATTAAGAGCTTTTTTTTTTTTTTTCCTTTGAGACGG





AGTCTTACTCTATTGCCCAGGCTGGAGAGTAGTGGCATGATCTTGGCTC





ACTACAACCTCCACCTTCCAGGTTCAAGCGATTCTCCTGCGTCGGCCTC





CCGAGTAGCTGGGATTACAGGTGCCTACCACCACACCGGCTAATTTTTT





TGTATTTTTAGTAGAGACAAGGTTTCGCCATGTTGGCCAGGCTGGTCTT





GAACTCCTGATCTCAAGCGATCTGCCCACCCTGGCCTCCCAAAGTGGTA





GGATTACAGGCAGGAGCCACCGCGCCCAGCCGTATTAAGAGCTTTTTAA





AGAGGTTTGTTGCAACTTTTGGGCCACCAGCATTTCCTTCTGCGGAAGT





TGGGCTACG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene KPNB1. In some embodiments, the sequences from gene KPNB1 comprise or consist of:









(SEQ ID NO: 1209)


GGCCTGAGGGCGGCGGTGCAGCGGGAGGGAGAAAGAGGGAGGGAAGGGA





AGTTAGGTTGCGCGGCGCTGCGTGTTCAGCAGGGGTGGGGGCGGGGGAG





TCGGGCCTAGGGGCCATGTGGAAGCTCCGGGCCCGGCCGCCCGCCCTGT





CTGACCCCGGCCGGCTTCTCCTTCCTATCCCGTGGCCCCTGTGGCTCTC





CCCACCTATTTCCTAGAGCTCTGCCCTTTCCTAGCTACCTTGGTCTGTA





CTCATGGCCAGGCCTGGCACCCGACTGCCTCTTAACCCATTTCCACTGA





TATTTTTCCCTCTTTGCTCGGTTATTCCTCTCTTGGGGTTTTTTCTTTT





TCTTTCTTTCTTTTTTTTTTTTCTTCTGACCTGGATGAATAGAGACCTA





ATCACTAGGGATCTGCACCCAGAAAACAGACTGAGGCTCATCTTTGAGA





TGGGGCCTGTTTGCTGATGAAATCACAGGGGAAAGTTCCTCGTTTTTCA





TATTTTAAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene KPNB1. In some embodiments, the sequences from gene KPNB1 comprise or consist of:









(SEQ ID NO: 1210)


TAATAGGAATTAAGTCTGAGTAACACTAACCATATGTGTTCCTTTGTGT





TATATAGGCAAAATGTCAACATCAACAAAGTGACAAGGCTTATTTTATA





CAGACTGCCTTTGAATGATTTTTTTGTTGTTTTTCATATGTGCCAATTT





TTACAACTAACCTGTTTATCCTTTCCTAGAAGAGATTTTTCCACTAGTA





TAGGCTGATAATTCCATTTAGGGTATTATTCCTGTTTCTGCTTTTGCAA





ATTTTGCAAAAAGCTTTTTATCTTATTCCTAACATTCTTAACATTCTTT





AGTGGTACATAATTAATAAAACTTTTAAGAATTATCTTCTGCTGTGATT





GCAGAGCTTGTTAGCAGTAAGAAAAATGTGTCTGCTTTTGACTTTTCCT





GCTAACTTTGCTGTTTGGGCACGGTTTTTGGTGGAGAGGTGTGTGTGTG





CTAAATTTTTAAACAAAGGGAGTAGTTTTTGAGGCCTGTGGATTACAAA





AGGTTACAA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene SPAG9. In some embodiments, the sequences from gene SPAG9 comprise or consist of:









(SEQ ID NO: 1211)


GTAAGGCTCTTCTCAAGGACAAAAAATATGATATCGTAAGCTGCATCAT





GTTGACTGTGCATTTGTTGCAATCCTCTGCCAAATTTAAATAATGGGGA





ATTATTTAGAGACTTGAAAATGACCTGGATCCCCATTAAATAAGTTTCT





GATTGGCTGGTTTTGTGATTGTAAGAAACTTTACAAAGTGAAGATGAGA





AATTTAGTGCTTGTAGGGTTTGAGTAATATTGTGTATGTATTGAGCTTG





AGTTATGTTTGTGCTAGAAGTACTATGTGTTTACTGTGATTAAAACTTA





CCCTTTTTTGGTGAATCTGCTATTTAATGGAAGTGTCAATTAAAAAAGA





AATAGACCCGAAAGTAGTTGTTTAAATTGAGCTTAATCAGAAAATGTAT





AAAATGTGTGTTAACATGGTAATATGTGTTCCAGTACTTGATTATAATA





AGCTGTTTTTAGAACTGGCATACTAAGCTTCCATTCACATCACACAGCC





ATAACCTTG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene SPAG9. In some embodiments, the sequences from gene SPAG9 comprise or consist of:









(SEQ ID NO: 1212)


TGTTGGCTGTCTAGCACATATTACAGTCCTCTGTCGGTAGACTAAGCTC





TTTGAGGGAGGGATGATATTTTATTTTTCTTAGTAGAATTAAAGTCAAT





CTTTAGCAGATTGACTGACACATGGTAGGTCAAAGTAACTACAGTATTT





ATTGAACTTAATTGTTGACTCCCCTCTGTGTATCCCATGAACTTCTTGG





GTCTCTTGTTCTTCAGAAAGAAAGGTAGAATACCTCACTGATAAATGTT





TCTTTTCTTTTCTTTTTGAATGTAGGGCATCACGAGAAAATCCAGCCAT





GCAGGAAAAAAAAAGGTCAAGCATTTGGCAGTTGTAAGTATTGAAAAAC





AAAGATGTTAGACAGCACATGAGACAAGAAAATTACAGTCAAAATTTAT





TTCCAAGAATGATTTTATGTCCATTTTCTTTCCTTCATTTTTTTTTTTT





AAACTAGGGGTAATGGTTAAGTAGCTAAACTGAACTTTTTTTTTTTTTT





TAATATATA






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene MAFG. In some embodiments, the sequences from gene MAFG comprise or consist of:









(SEQ ID NO: 1213)


GTATTGGGGGCTGGGCACCTGGACAGCAGGCTGGGGCGGGCTGTGCGGC





AAGGGCTCTTGTGAACTGGGCCGGGGGCGGGGGGGGGGGGGTGCGGCGA





GGGTGGTCTCCAGGGTACTGACCTGCTCCGTGTCTTGCAGAGAGCGCCT





GCTCGCTGTGCCCCCGGGTTATGACGACCCCCAATAAAGGAAACAAGGC





CTTGAAGGTGAGTGGGCAGGGACAGGAGCCTGTGGGCTGGGGTCGGGGG





GCACAGTGGGCGTGCCCGCTTGGCCTGTGACCACCTGCACTGTGCCCCA





GGTGAAGCGGGAGCCGGGTGAGAATGGCACCAGCCTGACGGATGAGGAG





CTGGTGACCATGTCGGTGCGGGAGCTGAACCAGCACCTGCGGGGCCTGT





CCAAGGAGGAGATCGTCCAGCTGAAGCAGCGCCGGCGCACGCTCAAGAA





CCGCGGCTACGCTGCCAGCTGCCGCGTGAAGCGGGTGACGCAGAAGGAG





GAGCTGGAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene MAFG. In some embodiments, the sequences from gene MAFG comprise or consist of:









(SEQ ID NO: 1214)


AAGCCACAGGATGGTTACTCCATCAGTGGAGGAGGCCGACTGGGAGGTC





TAGAGGGGCCTCGGGCCAGGCTCCTCCCAGCTGATGGCCTGCCACCTGG





AAGGTGGGGGAACTCCCTGGTCAGTCCTGCCAACAGACCAGAGCTAGAT





GGACGGCCCACCCCCAGCTTTTCAAAGTTAGGAGGCTGGGAGACTCGGG





GTTCCCTGTAGCCTCCTCTCTGGCCAGGTCCCCCGTGGGGCCCCTGTGC





ACCCAGGGAGCAGGGGGCTTTGTCCGAGGCCGGCTCAGGGGAGGTGGCT





GTAGAAGGTAGGACCCTGGAAGATGCACACACAGGGTCACTGGCTGGCC





CTCACCTGCTATTGGCCAGGCTGGGGGCTGCGGCTCTCGTGGGGCGCTG





TTGGCTGGCGGGCCAGGGAGTGCGGGCGCGGCGGGGAGGCAGAGGCAAT





GTGCTGAGTCAGCGTGACTCGCCAGGAACAGCCGCGCTCTGGGCAGTGC





CGGGGTTAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene RYR1. In some embodiments, the sequences from gene RYR1 comprise or consist of:









(SEQ ID NO: 1215)


GTCTGTGAAGCAGAGGTCAGAGGTGTTTGAAGGAAAAGGCAGGAGCGAG





GACTGTGTGATGGTGGAGGCAGGAGGTGGGGCTTCCTTTGGGGTTGGAG





GGATGTGGGGTCATCTGCAGAGGAGGACACAGAACAGGAGATTCCTGTC





CTTGGAGCAGGGTCAGTGTGTCTCAGGGGAGAGGCATGGGGGTGAGGCT





TCCTATGAGATTAGGTGGAACACGGGTTAGTGGGGAGGGTTAGGGTTAG





GGAAGTTTCTATGGAGTCTGGGTGGGTCTGAGGTGTTTGAGGGAGGAGG





CACAGAAGAGGTGTTTTCGTGATGTTGGTCTAGATTCAGTGTGTCTGAG





GGGGATGGCAGAGGGCAGGGCCTTCCTGTGGGGTGGGGGTGGGGTCTGG





CGTCTCAAGAGTGTGGGCATCCAGACTAGGGGAGGGAGTGTGGCAGGGA





ATGTTGCTGGGGTGGGGGGGTCTTCTGACCCCTCACTTACATCCCCCTC





CCACCCCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene RYR1. In some embodiments, the sequences from gene RYR1 comprise or consist of:









(SEQ ID NO: 1216)


TGAGGACCCCACCTGGGGGTGGGCGGGGTGGCAGAGATGGGCGAGAGGA





CCCAGGGGTCGTTTAGGGCACGGTGGCAAGGATGGGTGAGAGGACCCGG





GGGTCGCTTACCATCTGCTCTTCTTCCTCTCTCTGCCCTGCCCCCCACA





GGCCCTCAATTTGGATAAGTGGGTAGGTCCGGCCTTGGATGTCACCTGT





CCTTCCACCCCTCCTGACCCCAGGCAGGCCCGTCTCTCAGGCCCACGCC





CTGCACACAGGGGCTGGAGGGTCCTCCCCCACCCCAGTTGTCAGGGGCT





GTGGAGCCCAGAACAGGCCAGGCAGGAAGGAAGGAAGGGGATGGGGGCT





TCTGAGCCCCTGTCCCCGACGCCTGCTCCTTTCTCCTCTTTCCAGTCCT





TTCCCCCAACCCTGATCCCAGCTCCTCTCCGCATGGGACCTCTCCCACC





TCCCCCATCCCAAGCGTGAGTCCCTCTGGTCCAGGAGTGGGTCCACCAT





GTGATCACC






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene MYLK2. In some embodiments, the sequences from gene MYLK2 comprise or consist of:









(SEQ ID NO: 1217)


TGAATATCCCCTCCTGGGAGTGGGGAGGGGTCCTGTGGTTCTGTCCCTA





GGGGTCCTGCTTAATTCCCTTGTCTTAGGCATGGTTATCATCACATTCA





GTGCTGGGGAGTGTAGTTCTGACATTCAGTTTCCTCTGTCCTGTGAATT





GCCTCTACCGCCTTGTCCCCTGCAGAAACCAGAACCCTGCCACAATTCA





TACAAGACTGGAGTAAGAGAGACCCTCAAAGACTGCCTAGGTTGAACAT





ATCTTCTCTAGGCTTCTGGGGTCCGAAGTTCCCCTTACTCCATGGGCTT





GGGTCTAGCCAAGGGCAGGGTTGAGATGGCAGGCAGAAAGGCTAAAGGT





GTCAGGCAAAAGGCTGTGTATTTCCTAGGACATCCAGTATTTTCCTGTT





CTTCTTAATATAATTATTGGTAAGATATGAAAAGATGACTAATAACCAG





ATTGGAGGATCATTGATAATATTTTCATAACCATAATATAGCACATGTG





TGATGCCAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene MYLK2. In some embodiments, the sequences from gene MYLK2 comprise or consist of:









(SEQ ID NO: 1218)


CAACAGGTGCCAAGCTGGGGCAGGAGATGGAGGGAGGAGCTTGGGAAGG





GGGGTTTTGAATCCAGGACTGGGCAGGTTCCTCAGTGGGAGTTCTGTGC





CCCAGCCTTGTTTGCATGGTGCACGGGGGACCCAGAAATCAGAGGAATC





TCTGGCTAGGGTTGGGCTGGGTCCAGGGCCTGTATGAAGCCCTTCCTGG





AGAGACCAGGAAAATGTGAAGCCACCAGGGATGGCTGCTGGGGATGCAC





CTAAACAGTCAGGGCAAGGATGATGGGGGCCACCAAGGCCCACCCAAAG





GGCTGGAAGCCTCCCACAGAGGCCCTCCAAGGTCGTGGCAGCTCATCTC





TAGGGGCCATCAAGTGGCCCCAGAGACTCAGTGGGACAAGCATTTGCAA





GTTGTTGCCTGGGTGGGAACCTGGGCCCAGAGCTGGTCTCTGCCCAGCC





TGGGTGAGAGGGAAGGAAAGGAGGGTGGATCCTGATGGGTGTCTCACCT





CCTCTGCAG






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene CLTCL1. In some embodiments, the sequences from gene CLTCL1 comprise or consist of:









(SEQ ID NO: 1219)


GTAAACCCGATGGGATTGTTAGAAAGGAATCTTACCTCATTTTAACTGC





TTTGTTACCTTAAATATTGAACAAGTGTCACTAAGCTCATGGCGAAGTG





CTCCTCAATAGTATTCCACTTAATTCCAAAAGTTTTAGCAAAAGTTATG





TTTAGTGTGCCCTAGTAAGAGGCCCCTGAGCACCAGAGCTTCTTGGTAA





CATCACCCACAGCGCCAGACACACCGAGAGGAAGAAAATGCCCAGGCCT





TTGTCTTCATGCGACTAGGAACGCACAACAGCACTGTGCATGTTTCCCA





GGAAGGCTGTGTGGCCCCCTTGCCTGAGGGCCTGCTGCAGTGGTGCTTC





CGGTCATCCGACGACACAGGCTCGGGGGTTTTCCTCCTGTGAAACACAT





CAATGATAGAGGAGCTGATAATTAATAGAATTTTACTTCCTTGAAATCA





GGGAGTCTTGGGTGCTTAAAGCCTTCTAGTTATCTTGAAAAATATAGAA





TCTTCTTAT






In some embodiments, the intronic domain that promotes trans-splicing primarily in the muscle comprises or consists of sequences from the gene CLTCL1. In some embodiments, the sequences from gene CLTCL1 comprise or consist of:









(SEQ ID NO: 1220)


TTGATGATATCTAGGGAAATGTTTAAGCAAAAACTTTTGGCCAGTTCTT





TTTTCTTGCCTTTTGGAGAAATCAGGTTTTGTGTTTCCTCCCTTTTTCC





CTTCCACTTCCCTCGCCTTCACCTCTCCAAGACCTCCTTCCTCCCAAAG





CTCTCCTCGGTGGTGGGCTTATCTGTGACACCTTATCTGATGGTGTTCT





TTGGGAGTTCACCCCCAACTCATCTCCTCCTGTCTGTATCACTTGTGGC





GTCCTTGACACTGCCTAGTGTCATCCTGCACATGTCTTATCTCCACTGT





GGGCTGGTAATCTCTCAAGGAGAGTTGATTCACAGGCTTGGGTGGATCT





ATTACAGGCGTAGAAAAGCAAGCTAATGTTTCCTTCAAGTGTTTACCTA





ATGTTAACTTTTTTCTAAATTTTATTTAACTTCTCCTCTTTTGCCTTTG





GCTACAGTCAGTCCATTTCAGAGGAAAAAAATTTAATCAAATGCTTTTT





TTCTGTTTA






Replacement domains


Compositions comprising replacement domains disclosed herein include any strategies where replacement or insertion of RNA sequences could be an effective therapy. Exemplary replacement domains include without limitation sequences derived or isolated from the following genes (with gene accession IDs in brackets and associated diseases in parentheses) such as TNFRSF13B [ENSG00000240505] (common variable immune deficiency); ADA, CECR1 [ENSG00000196839, ENSG00000093072] (Adenosine deaminase deficiency); IL2RG [ENSG00000147168] (X-linked severe combined immunodeficiency); HBB [ENSG00000244734] (Beta-thassalemia); HBA1, HBA2 [ENSG00000206172, ENSG00000188536] (alpha-thassalemia); U2AF1 [ENSG00000160201] (myelodysplastic syndrome); SOD1, TARDBP, FUS, MATR3, SOD1, C9ORF72 [ENSG00000142168, ENSG00000120948, ENSG00000089280, ENSG00000015479, ENSG00000142168, ENSG00000147894] (Amyotrophic lateral sclerosis); MAPT, PGRN [ENSG00000186868, ENSG00000030582] (Frontotemporal dementia with parkinsonism); CDH23, MYO7A, USH2A [ENSG00000107736, ENSG00000137474, ENSG00000042781] (Usher's syndrome); GALC [ENSG00000054983] (Krabbe disease); SMPD1, NPC1, NPC2 [ENSG00000166311, ENSG00000141458, ENSG00000119655] (Niemann Pick disease); PRNP [ENSG00000171867] (prion disease); SCN1A [ENSG00000144285] (Dravet syndrome); PINK1, ATPGAP2 [ENSG00000158828] (early-onset Parkinson's disease); ATXN1, ATXN2, ATXN3, PLEKHG4, SPTBN2, CACNA1A, ATXN7, TTBK2, PPP2R2B, KCNC3, PRKCG, ITPR1, TBP, KCND1, FGF14 [ENSG00000124788, ENSG00000204842, ENSG00000066427, ENSG00000196155, ENSG00000173898, ENSG00000141837, ENSG00000163635, ENSG00000128881, ENSG00000156475, ENSG00000131398, ENSG00000126583, ENSG00000150995, ENSG00000112592, ENSG00000102057, ENSG00000102466] (spinocerebellar ataxias); SCN1A, SCN2A, CACNA1A, GRIN2B, GRIN2A, MECP2, FOXG1, SLC6A1, PRRT2, PTEN, KCNQ2, KCNQ3, STARD7, CLRN1 [ENSG00000144285, ENSG00000136531, ENSG00000141837, ENSG00000273079, ENSG00000183454, ENSG00000169057, ENSG00000176165, ENSG00000157103, ENSG00000167371, ENSG00000171862, ENSG00000075043, ENSG00000184156, ENSG00000084090, ENSG00000163646] (genetic epilepsy disorders); ATM [ENSG00000149311] (Ataxia-telangiectasia); GLB1 [ENSG00000170266] (GM1 gangliosidosis); GBA [ENSG00000177628] (Gaucher disease); GM2A [ENSG00000196743] (GM2 gangliosidosis); UBE3A [ENSG00000114062] (Angelman syndrome); SLC2A1 [ENSG00000117394] (glucose transporter deficiency type 1); LAM P2 [ENSG00000005893] (Danon disease); GLA [ENSG00000102393] (Fabry disease); PKD1, PKD2 [ENSG00000008710, ENSG00000118762] (Autosomal dominant polycystic kidney disease); GAA [ENSG00000171298] (Pompe disease); PCSK9, LDLR, APOB, APOE [ENSG00000169174, ENSG00000130164, ENSG00000084674, ENSG00000130203] (Familial hypercholesterolemia); MYOC, OPTN, TBK1, WDR36, CYPIB1 [ENSG00000034971, ENSG00000123240, ENSG00000183735, ENSG00000134987, ENSG00000138061] (Open Angle Glaucoma); IDUA [ENSG00000127415] (Hurler syndrome or Mucopolysaccharidosis 1); IDS [ENSG00000010404] (Hunter syndrome or Mucopolysaccharidosis 2); CLN3 [ENSG00000188603] (Batten disease); DMD [ENSG00000198947] (Duchenne muscular dystrophy); LMNA [ENSG00000160789] (Limb-girdle muscular dystrophy type 1B); DYSF [ENSG00000135636] (Limb-girdle muscular dystrophy type 2B); SGCA [ENSG00000108823] (Limb-girdle muscular dystrophy type 2D); SGCB [ENSG00000163069] (Limb-girdle muscular dystrophy type 2E); SGCG [ENSG00000102683] (Limb-girdle muscular dystrophy type 2C); SGCD [ENSG00000170624] (Limb-girdle muscular dystrophy type 2F); DUX4 [ENSG00000260596] (Facioscapulohumeral muscular dystrophy); F9 [ENSG00000101981] (Hemophilia B); F8 [ENSG00000185010] (Hemophilia A); USHA2A, RPGR, RP2, RHO, PRPF31, USH1F, PRPF3, PRPF6 [ENSG00000156313, ENSG00000102218, ENSG00000163914, ENSG00000105618, ENSG00000150275, ENSG00000117360, ENSG00000101161] (Retinitis pigmentosa); CFTR [ENSG00000001626] (cystic fibrosis); GJB2, GJB6, STRC, DFNA1, WFS1 [ENSG00000165474, ENSG00000121742, ENSG00000242866, ENSG00000131504, ENSG00000109501] (autosomal dominant hearing impairment); POU3F3 [ENSG00000198914] (nonsyndromic hearing loss).


In some embodiments, the replacement domain is codon optimized.


In addition to sequences derived from human genes, Replacement Domains can comprise sequences derived from other organisms in order to alter the stability, translation, processing, or localization of a target RNA. Exemplary replacement domains derived from non-human sources include without limitation sequences that increase protein production such as those derived or isolated from Woodchuck Hepatitis Virus (WHV) Post-transcriptional Regulatory Element (WPRE), triplex from MALAT1, the PRE of Hepatitis B virus (HPRE), and an iron response element of the form CAGYCX (Y=U or A; X=U, C, or A).


Antisense Domains

In some embodiments of the compositions of the disclosure, a pathogenic RNA molecule is a target RNA. In some embodiments, the target RNA comprises a target sequence that is complementary to an antisense domain of the trans-splicing RNA of the disclosure.


In some embodiments of the compositions and methods of the disclosure, the target sequence comprises or consists of between 5 and 500 nucleotides. In some embodiments, the target sequence comprises or consists of between 50 and 250 nucleotides. In some embodiments, the target sequence comprises or consists of between 5 and 50 nucleotides.


In some embodiments of the compositions and methods of the disclosure, a target sequence is contained within a single contiguous stretch of the target RNA. In some embodiments, the target sequence may consist of comprise of one or more nucleotides that are not spread among a single contiguous stretch of the target RNA.


In some embodiments of the disclosure, a antisense domain of the disclosure binds to a target sequence. In some embodiments of the disclosure, a antisense domain of the disclosure binds to a target RNA.


Vectors

In some embodiments of the compositions and methods of the disclosure, a vector comprises a trans-splicing nucleic acid of the disclosure. In some embodiments, the vector comprises at least one trans-splicing nucleic acid of the disclosure. In some embodiments, the vector comprises one or more trans-splicing nucleic acid(s) of the disclosure. In some embodiments, the vector comprises two or more trans-splicing nucleic acids of the disclosure.


In some embodiments of the compositions and methods of the disclosure, a vector of the disclosure is a viral vector. In some embodiments, the viral vector comprises a sequence isolated or derived from a retrovirus. In some embodiments, the viral vector comprises a sequence isolated or derived from a lentivirus. In some embodiments, the viral vector comprises a sequence isolated or derived from an adenovirus. In some embodiments, the viral vector comprises a sequence isolated or derived from an adeno-associated virus (AAV). In some embodiments, the viral vector is replication incompetent. In some embodiments, the viral vector is isolated or recombinant. In some embodiments, the viral vector is self-complementary.


In some embodiments of the compositions and methods of the disclosure, the viral vector comprises a sequence isolated or derived from an adeno-associated virus (AAV). In some embodiments, the viral vector comprises an inverted terminal repeat sequence or a capsid sequence that is isolated or derived from an AAV of serotype AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV11 or AAV12. In some embodiments, the viral vector is replication incompetent. In some embodiments, the viral vector is isolated or recombinant (rAAV). In some embodiments, the viral vector is self-complementary (scAAV).


In some embodiments of the compositions and methods of the disclosure, a vector of the disclosure is a non-viral vector. In some embodiments, the vector comprises or consists of a nanoparticle, a micelle, a liposome or lipoplex, a polymersome, a polyplex or a dendrimer.


In some embodiments, the vector is an expression vector or recombinant expression system. As used herein, the term“recombinant expression system” refers to a genetic construct for the expression of certain genetic material formed by recombination.


In some embodiments, the liposome, lipoplex, or nanoparticle can further comprise a non-cationic lipid, a PEG conjugated lipid, a sterol, or any combination thereof.


In some embodiments, the the liposome, lipoplex, or nanoparticle further comprises a non-cationic lipid, wherein the non-ionic lipid is selected from the group consisting of distearoyl-sn-glycero-phosphoethanolamine, distearoylphosphatidylcholine (DSPC), dioleoylphosphatidylcholine (DOPC), dipalmitoylphosphatidylcholine (DPPC), dioleoylphosphatidylglycerol (DOPG), dipalmitoylphosphatidylglycerol (DPPG), dioleoyl-phosphatidylethanolamine (DOPE), palmitoyloleoylphosphatidylcholine (POPC), palmitoyloleoylphosphatidylethanolamine (POPE), dioleoyl-phosphatidylethanolamine 4-(N-maleimidomethyl)-cyclohexane-1-carboxylate (DOPE-mal), dipalmitoyl phosphatidyl ethanolamine (DPPE), dimyristoylphosphoethanolamine (DMPE), distearoyl-phosphatidyl-ethanolamine (DSPE), monomethyl-phosphatidylethanolamine (such as 16-O-monomethyl PE), dimethyl-phosphatidylethanolamine (such as 16-O-dimethyl PE), 18-1-trans PE, I-stearoyl-2-oleoyl-phosphatidyethanolamine (SOPE), hydrogenated soy phosphatidylcholine (HSPC), egg phosphatidylcholine (EPC), dioleoylphosphatidylserine (DOPS), sphingomyelin (SM), dimyristoyl phosphatidylcholine (DMPC), dimyristoyl phosphatidylglycerol (DMPG), distearoylphosphatidylglycerol (DSPG), dierucoylphosphatidylcholine (DEPC), palmitoyloleyolphosphatidylglycerol (POPG), dielaidoyl-phosphatidylethanolamine (DEPE), lecithin, phosphatidylethanolamine, lysolecithin, lysophosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, sphingomyelin, egg sphingomyelin (ESM), cephalin, cardiolipin, phosphatidicacid, cerebrosides, dicetylphosphate, lysophosphatidylcholine, dilinoleoylphosphatidylcholine and non-cationic lipids described, for example, in WO2017/099823 or US2018/0028664.


In some embodiments, the liposome, lipoplex, or nanoparticle further comprises a conjugated lipid, wherein the conjugated lipid, wherein the conjugated-lipid is selected from the group consisting of PEG-diacylglycerol (DAG) (such as I-(monomethoxy-polyethyleneglycol)-2,3-dimyristoylglycerol (PEG-DMG)), PEG-dialkyloxypropyl (DAA), PEG-phospholipid, PEG-ceramide (Cer), a pegylated phosphatidylethanoloamine (PEG-PE), PEG succinate diacylglycerol (PEGS-DAG) (such as 4-O-(2′,3′-di(tetradecanoyloxy)propyl-1-O-(w-methoxy(polyethoxy)ethyl) butanedioate (PEG-S-DMG)), PEG dialkoxypropylcarbam, N-(carbonyl-methoxypoly ethylene glycol 2000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine sodium salt.


In some embodiments, the liposome, lipoplex, or nanoparticle further comprises cholesterol or a cholesterol derivative.


In some embodiments, the liposome, lipoplex, or nanoparticle further comprises an ionizable lipid, a non-cationic lipid, a conjugated lipid that inhibits aggregation of particles, and a sterol. The amount of the ionizable lipid, the non-cationic lipid, the conjugated lipid that inhibits aggregation of particles, and the sterol can be varied independently. In some embodiments, the lipid nanoparticle comprises an ionizable lipid in an amount from about 20 mol % to about 90 mol % of the total lipid present in the particle, a non-cationic lipid in an amount from about 5 mol % to about 30 mol % of the total lipid present in the particle, a conjugated lipid that inhibits aggregation of particles in an amount from about 0.5 mol % to about 20 mol % of the total lipid present in the particle, and a sterol in an amount from about 20 mol % to about 50 mol % of the total lipid present in the particle.


The ratio of total lipid to DNA vector can be varied as desired. For example, the total lipid to DNA vector (mass or weight) ratio can be from about 10:1 to about 30:1.


In some embodiments of the compositions and methods of the disclosure, an expression vector, viral vector or non-viral vector provided herein, includes without limitation, an expression control element. An“expression control element” as used herein refers to any sequence that regulates the expression of a coding sequence, such as a gene. Exemplary expression control elements include but are not limited to promoters, enhancers, microRNAs, post-transcriptional regulatory elements, polyadenylation signal sequences, and introns. Expression control elements may be constitutive, inducible, repressible, or tissue-specific, for example. A “promoter” is a control sequence that is a region of a polynucleotide sequence at which initiation and rate of transcription are controlled. It may contain genetic elements at which regulatory proteins and molecules may bind such as RNA polymerase and other transcription factors. In some embodiments, expression control by a promoter is tissue-specific. Non-limiting exemplary promoters include CMV, CBA, CAG, Cbh, EF-1a, PGK, UBC, GUSB, UCOE, hAAT, TBG, Desmin, MCK, C5-12, NSE, Synapsin, PDGF, MecP2, CaMKII, mGluR2, NFL, NFH, n132, PPE, ENK, EAAT2, GFAP, MBP, and U6 promoters. An“enhancer” is a region of DNA that can be bound by activating proteins to increase the likelihood or frequency of transcription. Non-limiting exemplary enhancers and posttranscriptional regulatory elements include the CMV enhancer and WPRE.


Nucleic Acids

Provided herein are the nucleic acid sequences encoding the trans-splicing nucleic acids disclosed herein for use in gene transfer and expression techniques described herein. It should be understood, although not always explicitly stated that the sequences provided herein can be used to provide the expression product as well as substantially identical sequences that produce a protein that has the same biological properties. These“biologically equivalent” or“biologically active” or“equivalent” polypeptides are encoded by equivalent polynucleotides as described herein. They may possess at least 60%, or alternatively, at least 65%, or alternatively, at least 70%, or alternatively, at least 75%, or alternatively, at least 80%, or alternatively at least 85%, or alternatively at least 90%, or alternatively at least 95% or alternatively at least 98%, identical nucleic acid sequence to the reference nucleic acid sequence when compared using sequence identity methods run under default conditions. Specific sequences are provided as examples of particular embodiments. Additionally, an equivalent polynucleotide is one that hybridizes under stringent conditions to the reference polynucleotide or its complement.


The nucleic acid sequences (e.g., polynucleotide sequences) disclosed herein may be codon-optimized which is a technique well known in the art. Codon optimization refers to the fact that different cells differ in their usage of particular codons. This codon bias corresponds to a bias in the relative abundance of particular tRNAs in the cell type. By altering the codons in the sequence to match with the relative abundance of corresponding tRNAs, it is possible to increase expression. It is also possible to decrease expression by deliberately choosing codons for which the corresponding tRNAs are known to be rare in a particular cell type. Codon usage tables are known in the art for mammalian cells, as well as for a variety of other organisms. Based on the genetic code, nucleic acid sequences coding for various replacement domains can be generated. In some embodiments, such a sequence is optimized for expression in a host or target cell, such as a host cell used to express the trans-splicing RNA containing a replacement domain in which the disclosed methods are practiced (such as in a mammalian cell, e.g., a human cell). Codon preferences and codon usage tables for a particular species can be used to engineer isolated nucleic acid molecules encoding a replacement domain (such as one encoding a protein having at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to its corresponding wild-type protein) that takes advantage of the codon usage preferences of that particular species. For example, the replacement domains disclosed herein can be designed to have codons that are preferentially used by a particular organism of interest. In one example, a replacement domain nucleic acid sequence is optimized for expression in human cells, such as one having at least 70%, at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 98%, or at least 99% sequence identity to its corresponding wild-type or originating nucleic acid sequence. In some embodiments, an isolated trans-splicing nucleic acid molecule encoding at least one replacement domain (which can be part of a vector) includes at least one replacement domain coding sequence that is codon optimized for expression in a eukaryotic cell, or at least one replacement domain coding sequence codon optimized for expression in a human cell. In one embodiment, such a codon optimized replacement domain coding sequence has at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to its corresponding wild-type or originating sequence. In another embodiment, a eukaryotic cell codon optimized nucleic acid sequence encodes a replacement domain having at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to its corresponding wild-type or originating protein. In another embodiment, a variety of clones containing functionally equivalent nucleic acids may be routinely generated, such as nucleic acids which differ in sequence but which encode the same replacement domain protein sequence. Silent mutations in the coding sequence result from the degeneracy (i.e., redundancy) of the genetic code, whereby more than one codon can encode the same amino acid residue. Thus, for example, leucine can be encoded by CTT, CTC, CTA, CTG, TTA, or TTG; serine can be encoded by TCT, TCC, TCA, TCG, AGT, or AGC; asparagine can be encoded by AAT or AAC; aspartic acid can be encoded by GAT or GAC; cysteine can be encoded by TGT or TGC; alanine can be encoded by GCT, GCC, GCA, or GCG; glutamine can be encoded by CAA or CAG; tyrosine can be encoded by TAT or TAC; and isoleucine can be encoded by ATT, ATC, or ATA. Tables showing the standard genetic code can be found in various sources (see, for example, Stryer, 1988, Biochemistry, 3.sup.rd Edition, W.H.5 Freeman and Co., NY).


“Hybridization” refers to a reaction in which one or more polynucleotides react to form a complex that is stabilized via hydrogen bonding between the bases of the nucleotide residues. The hydrogen bonding may occur by Watson-Crick base pairing, Hoogstein binding, or in any other sequence-specific manner. The complex may comprise two strands forming a duplex structure, three or more strands forming a multi-stranded complex, a single self-hybridizing strand, or any combination of these. A hybridization reaction may constitute a step in a more extensive process, such as the initiation of a PC reaction, or the enzymatic cleavage of a polynucleotide by a ribozyme.


Examples of stringent hybridization conditions include: incubation temperatures of about 25° C. to about 37° C.; hybridization buffer concentrations of about 6×SSC to about 10×SSC; formamide concentrations of about 0% to about 25%; and wash solutions from about 4×SSC to about 8×SSC. Examples of moderate hybridization conditions include: incubation temperatures of about 40° C. to about 50° C.; buffer concentrations of about 9×SSC to about 2×SSC; formamide concentrations of about 30% to about 50%; and wash solutions of about 5×SSC to about 2×SSC. Examples of high stringency conditions include: incubation temperatures of about 55° C. to about 68° C.; buffer concentrations of about 1×SSC to about 0.1×SSC;


“Homology” or “identity” or “similarity” refers to sequence similarity between two peptides or between two nucleic acid molecules. Homology can be determined by comparing a position in each sequence which may be aligned for purposes of comparison. When a position in the compared sequence is occupied by the same base or amino acid, then the molecules are homologous at that position. A degree of homology between sequences is a function of the number of matching or homologous positions shared by the sequences. An “unrelated” or “non-homologous” sequence shares less than 40% identity, or alternatively less than 25% identity, with one of the sequences of the present invention.


Methods of Use

The disclosure provides a method of modifying the composition of a target RNA of the disclosure primarily in a specific tissue comprising contacting the composition and the target RNA under conditions suitable for trans-splicing among the composition and the target RNA.


The disclosure provides a method of modifying the composition of a target RNA of the disclosure primarily in a specific tissue comprising contacting the composition and a cell comprising the target RNA under conditions suitable for trans-splicing among the composition and the target RNA.


The disclosure provides a method of modifying the composition of a protein encoded by a target RNA of the disclosure primarily in a specific tissue comprising contacting the composition and the target RNA under conditions suitable for trans-splicing among the composition and the target RNA.


The disclosure provides a method of modifying the composition of a protein encoded by a target RNA of the disclosure primarily in a specific tissue comprising contacting the composition and a cell comprising the target RNA under conditions suitable for trans-splicing among the composition and the target RNA.


The disclosure provides a method of modifying the composition of a target RNA of the disclosure primarily in liver tissue comprising contacting the composition and a liver cell comprising the target RNA under conditions suitable for trans-splicing among the composition and the target RNA.


The disclosure provides a method of modifying the composition of a protein encoded by a target RNA of the disclosure primarily in liver tissue comprising contacting the composition and a liver cell comprising the target RNA under conditions suitable for trans-splicing among the composition and the target RNA.


The disclosure provides a method of modifying the composition of a target RNA of the disclosure primarily in muscle tissue comprising contacting the composition and a muscle cell comprising the target RNA under conditions suitable for trans-splicing among the composition and the target RNA.


The disclosure provides a method of modifying the composition of a protein encoded by a target RNA of the disclosure primarily in muscle tissue comprising contacting the composition and a muscle cell comprising the target RNA under conditions suitable for trans-splicing among the composition and the target RNA.


The disclosure provides a method of modifying the composition of a target RNA of the disclosure primarily in brain tissue comprising contacting the composition and a brain cell comprising the target RNA under conditions suitable for trans-splicing among the composition and the target RNA.


The disclosure provides a method of modifying the composition of a protein encoded by a target RNA of the disclosure primarily in brain tissue comprising contacting the composition and a brain cell comprising the target RNA under conditions suitable for trans-splicing among the composition and the target RNA.


The disclosure provides a method of modifying the composition of a target RNA of the disclosure primarily in heart tissue comprising contacting the composition and a heart cell comprising the target RNA under conditions suitable for trans-splicing among the composition and the target RNA.


The disclosure provides a method of modifying the composition of a protein encoded by a target RNA of the disclosure primarily in heart tissue comprising contacting the composition and a heart cell comprising the target RNA under conditions suitable for trans-splicing among the composition and the target RNA.


The disclosure provides a method of modifying the composition of a target RNA of the disclosure primarily in retina tissue comprising contacting the composition and a retina cell comprising the target RNA under conditions suitable for trans-splicing among the composition and the target RNA.


The disclosure provides a method of modifying the composition of a protein encoded by a target RNA of the disclosure primarily in retina tissue comprising contacting the composition and a retina cell comprising the target RNA under conditions suitable for trans-splicing among the composition and the target RNA.


The disclosure provides a method of modifying the composition of a target RNA of the disclosure primarily in a specific tissue comprising contacting the composition and a cell comprising the target RNA under conditions suitable for trans-splicing among the composition and the target RNA. In some embodiments, the cell is in vivo, in vitro, ex vivo or in situ. In some embodiments, the composition comprises a vector comprising composition comprising a guide RNA of the disclosure and a fusion protein of the disclosure. In some embodiments, the vector is an AAV.


The disclosure provides a method of modifying the composition of a protein encoded by a target RNA of the disclosure primarily in a specific tissue comprising contacting the composition and a cell comprising the target RNA under conditions suitable for trans-splicing among the composition and the target RNA. In some embodiments, the cell is in vivo, in vitro, ex vivo or in situ. In some embodiments, the composition comprises a vector comprising composition comprising a guide RNA of the disclosure and a fusion protein of the disclosure. In some embodiments, the vector is an AAV.


The disclosure provides a method of treating a disease or disorder comprising administering to a subject a therapeutically effective amount of a composition of the disclosure.


The disclosure provides a method of treating a disease or disorder comprising administering to a subject a therapeutically effective amount of a composition of the disclosure, wherein the composition comprises a vector comprising composition comprising a guide RNA of the disclosure and a fusion protein of the disclosure and wherein the composition modifies a level of expression of an RNA molecule of the disclosure or a protein encoded by the RNA molecule.


The disclosure provides a method of treating a disease or disorder comprising administering to a subject a therapeutically effective amount of a composition of the disclosure, wherein the composition comprises a vector comprising composition comprising a guide RNA of the disclosure and a fusion protein of the disclosure and wherein the composition modifies an activity of a protein encoded by an RNA molecule.


In some embodiments of the compositions and methods of the disclosure, a disease or disorder of the disclosure includes, but is not limited to, a genetic disease or disorder. In some embodiments, the genetic disease or disorder is a single-gene disease or disorder. In some embodiments, the single-gene disease or disorder is an autosomal dominant disease or disorder, an autosomal recessive disease or disorder, an X-chromosome linked (X-linked) disease or disorder, an X-linked dominant disease or disorder, an X-linked recessive disease or disorder, a Y-linked disease or disorder or a mitochondrial disease or disorder. In some embodiments, the singe-gene disease or disorder is, but not limited to, common variable immune deficiency, Adenosine deaminase deficiency, X-linked severe combined immunodeficiency, Beta-thassalemia, alpha-thassalemia, myelodysplastic syndrome, Amyotrophic lateral sclerosis, Frontotemporal dementia with parkinsonism, Usher's syndrome, Krabbe disease, Niemann Pick disease, prion disease, Dravet syndrome, early-onset Parkinson's disease, spinocerebellar ataxias, genetic epilepsy disorders, Ataxia-telangiectasia, GM1 gangliosidosis, Gaucher disease, GM2 gangliosidosis, Angelman syndrome, glucose transporter deficiency type 1, Danon disease, Fabry disease, Autosomal dominant polycystic kidney disease, Pompe disease, Familial hypercholesterolemia, Open Angle Glaucoma, Hurler syndrome or Mucopolysaccharidosis 1, Hunter syndrome or Mucopolysaccharidosis 2, Batten disease, Duchenne muscular dystrophy, Limb-girdle muscular dystrophy type 1B, Limb-girdle muscular dystrophy type 2B, Limb-girdle muscular dystrophy type 2D, Limb-girdle muscular dystrophy type 2E, Limb-girdle muscular dystrophy type 2C, Limb-girdle muscular dystrophy type 2F, Facioscapulohumeral muscular dystrophy, Hemophilia B, Hemophilia A, Retinitis pigmentosa, cystic fibrosis, autosomal dominant hearing impairment, and non-syndromic hearing loss. In some embodiments, the genetic disease or disorder is a multiple-gene disease or disorder. In some embodiments, the genetic disease or disorder is a multiple-gene disease or disorder. In some embodiments, the single-gene disease or disorder is an autosomal dominant disease or disorder including, but not limited to, Huntington's disease, neurofibromatosis type 1, neurofibromatosis type 2, Marfan syndrome, hereditary nonpolyposis colorectal cancer, hereditary multiple exostoses, Von Willebrand disease, and acute intermittent porphyria. In some embodiments, the single-gene disease or disorder is an autosomal recessive disease or disorder including, but not limited to, Albinism, Medium-chain acyl-CoA dehydrogenase deficiency, cystic fibrosis, sickle-cell disease, Tay-Sachs disease, Niemann-Pick disease, spinal muscular atrophy, and Roberts syndrome. In some embodiments, the single-gene disease or disorder is X-linked disease or disorder including, but not limited to, muscular dystrophy, Duchenne muscular dystrophy, Hemophilia, Adrenoleukodystrophy (ALD), Rett syndrome, and Hemophilia A. In some embodiments, the single-gene disease or disorder is a mitochondrial disorder including, but not limited to, Leber's hereditary optic neuropathy.


In some embodiments of the compositions and methods of the disclosure, a disease or disorder of the disclosure includes, but is not limited to, an immune disease or disorder. In some embodiments, the immune disease or disorder is an immunodeficiency disease or disorder including, but not limited to, B-cell deficiency, T-cell deficiency, neutropenia, asplenia, complement deficiency, acquired immunodeficiency syndrome (AIDS) and immunodeficiency due to medical intervention (immunosuppression as an intended or adverse effect of a medical therapy). In some embodiments, the immune disease or disorder is an autoimmune disease or disorder including, but not limited to, Achalasia, Addison's disease, Adult Still's disease, Agammaglobulinemia, Alopecia areata, Amyloidosis, Anti-GBM/Anti-TBM nephritis, Antiphospholipid syndrome, Autoimmune angioedema, Autoimmune dysautonomia, Autoimmune encephalomyelitis, Autoimmune hepatitis, Autoimmune inner ear disease (AIED), Autoimmune myocarditis, Autoimmune oophoritis, Autoimmune orchitis, Autoimmune pancreatitis, Autoimmune retinopathy, Autoimmune urticaria, Axonal & neuronal neuropathy (AMAN), Baló disease, Behcet's disease, Benign mucosal pemphigoid, Bullous pemphigoid, Castleman disease (CD), Celiac disease, Chagas disease, Chronic inflammatory demyelinating polyneuropathy (CIDP), Chronic recurrent multifocal osteomyelitis (CRMO), Churg-Strauss Syndrome (CSS) or Eosinophilic Granulomatosis (EGPA), Cicatricial pemphigoid, Cogan's syndrome, Cold agglutinin disease, Congenital heart block, Coxsackie myocarditis, CREST syndrome, Crohn's disease, Dermatitis herpetiformis, Dermatomyositis, Devic's disease (neuromyelitis optica), Discoid lupus, Dressler's syndrome, Endometriosis, Eosinophilic esophagitis (EoE), Eosinophilic fasciitis, Erythema nodosum, Essential mixed cryoglobulinemia, Evans syndrome, Fibromyalgia, Fibrosing alveolitis, Giant cell arteritis (temporal arteritis), Giant cell myocarditis, Glomerulonephritis, Goodpasture's syndrome, Granulomatosis with Polyangiitis, Graves' disease, Guillain-Barre syndrome, Hashimoto's thyroiditis, Hemolytic anemia, Henoch-Schonlein purpura (HSP), Herpes gestationis or pemphigoid gestationis (PG), Hidradenitis Suppurativa (HS) (Acne Inversa), Hypogammalglobulinemia, IgA Nephropathy, IgG4-related sclerosing disease, Immune thrombocytopenic purpura (ITP), Inclusion body myositis (IBM), Interstitial cystitis (IC), Juvenile arthritis, Juvenile diabetes (Type 1 diabetes), Juvenile myositis (JM), Kawasaki disease, Lambert-Eaton syndrome, Leukocytoclastic vasculitis, Lichen planus, Lichen sclerosus, Ligneous conjunctivitis, Linear IgA disease (LAD), Lupus, Lyme disease chronic, Meniere's disease, Microscopic polyangiitis (MPA), Mixed connective tissue disease (MCTD), Mooren's ulcer, Mucha-Habermann disease, Multifocal Motor Neuropathy (MMN) or MMNCB, Multiple sclerosis, Myasthenia gravis, Myositis, Narcolepsy, Neonatal Lupus, Neuromyelitis optica, Neutropenia, Ocular cicatricial pemphigoid, Optic neuritis, Palindromic rheumatism (PR), PANDAS, Paraneoplastic cerebellar degeneration (PCD), Paroxysmal nocturnal hemoglobinuria (PNH), Parry Romberg syndrome, Pars planitis (peripheral uveitis), Parsonnage-Turner syndrome, Pemphigus, Peripheral neuropathy, Perivenous encephalomyelitis, Pernicious anemia (PA), POEMS syndrome, Polyarteritis nodosa, Polyglandular syndromes type I, II, III, Polymyalgia rheumatica, Polymyositis, Postmyocardial infarction syndrome, Postpericardiotomy syndrome, Primary biliary cirrhosis, Primary sclerosing cholangitis, Progesterone dermatitis, Psoriasis, Psoriatic arthritis, Pure red cell aplasia (PRCA), Pyoderma gangrenosum, Raynaud's phenomenon, Reactive Arthritis, Reflex sympathetic dystrophy, Relapsing polychondritis, Restless legs syndrome (RLS), Retroperitoneal fibrosis, Rheumatic fever, Rheumatoid arthritis, Sarcoidosis, Schmidt syndrome, Scleritis, Scleroderma, Sjögren's syndrome, Sperm & testicular autoimmunity, Stiff person syndrome (SPS), Subacute bacterial endocarditis (SBE), Susac's syndrome, Sympathetic ophthalmia (SO), Takayasu's arteritis, Temporal arteritis/Giant cell arteritis, Thrombocytopenic purpura (TTP), Tolosa-Hunt syndrome (THS), Transverse myelitis, Type 1 diabetes, Ulcerative colitis (UC), Undifferentiated connective tissue disease (UCTD), Uveitis, Vasculitis, Vitiligo, Vogt-Koyanagi-Harada Disease, or Wegener's granulomatosis.


In some embodiments of the compositions and methods of the disclosure, a disease or disorder of the disclosure includes, but is not limited to, an inflammatory disease or disorder.


In some embodiments of the compositions and methods of the disclosure, a disease or disorder of the disclosure includes, but is not limited to, a metabolic disease or disorder.


In some embodiments of the compositions and methods of the disclosure, a disease or disorder of the disclosure includes, but is not limited to, a degenerative or a progressive disease or disorder. In some embodiments, the degenerative or a progressive disease or disorder includes, but is not limited to, amyotrophic lateral sclerosis (ALS), Huntington's disease, Alzheimer's disease, and aging.


In some embodiments of the compositions and methods of the disclosure, a disease or disorder of the disclosure includes, but is not limited to, an infectious disease or disorder.


In some embodiments of the compositions and methods of the disclosure, a disease or disorder of the disclosure includes, but is not limited to, a pediatric or a developmental disease or disorder.


In some embodiments of the compositions and methods of the disclosure, a disease or disorder of the disclosure includes, but is not limited to, a cardiovascular disease or disorder.


In some embodiments of the compositions and methods of the disclosure, a disease or disorder of the disclosure includes, but is not limited to, a proliferative disease or disorder. In some embodiments, the proliferative disease or disorder is a cancer. In some embodiments, the cancer includes, but is not limited to, Acute Lymphoblastic Leukemia (ALL), Acute Myeloid Leukemia (AML), Adrenocortical Carcinoma, AIDS-Related Cancers, Kaposi Sarcoma (Soft Tissue Sarcoma), AIDS-Related Lymphoma (Lymphoma), Primary CNS Lymphoma (Lymphoma), Anal Cancer, Appendix Cancer, Gastrointestinal Carcinoid Tumors, Astrocytomas, Atypical Teratoid/Rhabdoid Tumor, Central Nervous System (Brain Cancer), Basal Cell Carcinoma, Bile Duct Cancer, Bladder Cancer, Bone Cancer, Ewing Sarcoma, Osteosarcoma, Malignant Fibrous Histiocytoma, Brain Tumors, Breast Cancer, Burkitt Lymphoma, Carcinoid Tumor, Carcinoma, Cardiac (Heart) Tumors, Embryonal Tumors, Germ Cell Tumor, Primary CNS Lymphoma, Cervical Cancer, Cholangiocarcinoma, Chordoma, Chronic Lymphocytic Leukemia (CLL), Chronic Myelogenous Leukemia (CML), Chronic Myeloproliferative Neoplasms, Colorectal Cancer, Craniopharyngioma, Cutaneous T-Cell Lymphoma, Ductal Carcinoma In Situ, Embryonal Tumors, Endometrial Cancer (Uterine Cancer), Ependymoma, Esophageal Cancer, Esthesioneuroblastoma (Head and Neck Cancer), Ewing Sarcoma (Bone Cancer), Extracranial Germ Cell Tumor, Extragonadal Germ Cell Tumor, Eye Cancer, Childhood Intraocular Melanoma, Intraocular Melanoma, Retinoblastoma, Fallopian Tube Cancer, Fibrous Histiocytoma of Bone, Malignant, and Osteosarcoma, Gallbladder Cancer, Gastric (Stomach) Cancer, Gastrointestinal Carcinoid Tumor, Gastrointestinal Stromal Tumors (GIST) (Soft Tissue Sarcoma), Childhood Gastrointestinal Stromal Tumors, Germ Cell Tumors, Childhood Extracranial Germ Cell Tumors, Extragonadal Germ Cell Tumors, Ovarian Germ Cell Tumors, Testicular Cancer, Gestational Trophoblastic Disease, Hairy Cell Leukemia, Head and Neck Cancer, Heart Tumors, Hepatocellular (Liver) Cancer, Histiocytosis, Hodgkin Lymphoma, Hypopharyngeal Cancer (Head and Neck Cancer), Intraocular Melanoma, Islet Cell Tumors, Pancreatic Neuroendocrine Tumors, Kaposi Sarcoma (Soft Tissue Sarcoma), Kidney (Renal Cell) Cancer, Langerhans Cell Histiocytosis, Laryngeal Cancer (Head and Neck Cancer), Leukemia, Lip and Oral Cavity Cancer (Head and Neck Cancer), Liver Cancer, Lung Cancer (Non-Small Cell and Small Cell), Childhood Lung Cancer, Lymphoma, Male Breast Cancer, Malignant Fibrous Histiocytoma of Bone and Osteosarcoma, Melanoma, Merkel Cell Carcinoma (Skin Cancer), Mesothelioma, Metastatic Squamous Neck Cancer with Occult Primary (Head and Neck Cancer), Midline Tract Carcinoma With NUT Gene Changes, Mouth Cancer (Head and Neck Cancer), Multiple Endocrine Neoplasia Syndromes, Multiple Myeloma/Plasma Cell Neoplasms, Mycosis Fungoides (Lymphoma), Myelodysplastic Syndromes, Myelodysplastic/Myeloproliferative Neoplasms, Nasal Cavity and Paranasal Sinus Cancer (Head and Neck Cancer), Nasopharyngeal Cancer (Head and Neck Cancer), Neuroblastoma, Non-Hodgkin Lymphoma, Non-Small Cell Lung Cancer, Oral Cancer, Lip and Oral Cavity Cancer and Oropharyngeal Cancer, Osteosarcoma and Malignant Fibrous Histiocytoma of Bone, Ovarian Cancer, Pancreatic Cancer, Pancreatic Neuroendocrine Tumors (Islet Cell Tumors), Papillomatosis, Paraganglioma, Parathyroid Cancer, Penile Cancer, Pharyngeal Cancer (Head and Neck Cancer), Pheochromocytoma, Plasma Cell Neoplasm/Multiple Myeloma, Pleuropulmonary Blastoma, Pregnancy and Breast Cancer, Primary Central Nervous System (CNS) Lymphoma, Primary Peritoneal Cancer, Prostate Cancer, Rectal Cancer, Recurrent Cancer, Renal Cell (Kidney) Cancer, Retinoblastoma, Rhabdomyosarcoma, Childhood (Soft Tissue Sarcoma), Salivary Gland Cancer (Head and Neck Cancer), Sarcoma, Childhood Rhabdomyosarcoma (Soft Tissue Sarcoma), Childhood Vascular Tumors (Soft Tissue Sarcoma), Ewing Sarcoma (Bone Cancer), Kaposi Sarcoma (Soft Tissue Sarcoma), Osteosarcoma (Bone Cancer), Uterine Sarcoma, Sézary Syndrome, Lymphoma, Skin Cancer, Small Cell Lung Cancer, Small Intestine Cancer, Soft Tissue Sarcoma, Squamous Cell Carcinoma of the Skin, Squamous Neck Cancer, Stomach (Gastric) Cancer, T-Cell Lymphoma, Testicular Cancer, Throat Cancer (Head and Neck Cancer), Nasopharyngeal Cancer, Oropharyngeal Cancer, Hypopharyngeal Cancer, Thymoma and Thymic Carcinoma, Thyroid Cancer, Transitional Cell Cancer of the Renal Pelvis and Ureter, Renal Cell Cancer, Urethral Cancer, Uterine Sarcoma, Vaginal Cancer, Vascular Tumors (Soft Tissue Sarcoma), Vulvar Cancer, Wilms Tumor and Other Childhood Kidney Tumors.


In some embodiments of the compositions and methods of the disclosure, a disease or disorder of the disclosure includes, but is not limited to, a proliferative disease or disorder. In some embodiments, the proliferative disease or disorder is a cancer. In some embodiments, the cancer involves the present of a gene fusion that produces a chimeric RNA with sequences derived from two genes due to a deletion or translocation of DNA. Gene fusions pairs include but are not limited to: MAN2A1 and FER, DNAJB1 and PRKACA, BCR-ABL1, TMPRSS2 and ERG, EWSR1 and FLI1, PML and RARA, EML4 and ALK, KIAA1549 and BRAF, CCDC6 and RET, SS18 and SSX1, RUNX1 and RUNX1T1, PAX3 and FOXO1, NCOA4 and RET, ETV6 and RUNX1, FUS and DDIT3, SS18 and SSX2, NPM1 and ALK, KMT2A and AFF1, TCF3 and PBX1, STIL and TALI, COL1A1 and PDGFB, CRTC1 and MAML2, NAB2 and STATE, EWSR1 and ATF1, ETV6 and NTRK3, EWSR1 and ERG, EWSR1 and WT1, DNAJB1 and PRKACA, PAX7 and FOXO1, FUS and CREB3L2, CBFA2T3 and GLIS2, PAX8 and PPARG, KMT2A and MLLT1, EWSR1 and NR4A3, KMT2A and MLLT3, ASPSCR1 and TFE3, HMGA2 and LPP, JAZF1 and SUZ12, KIF5B and RET, FUS and ERG, SLC45A3 and ERG, NUP214 and ABL1, SET and NUP214, CD74 and ROS1, ETV6 and ABL1, TPM3 and NTRK1, PRKAR1A and RET, EWSR1 and CREB1, KMT2A and AFDN, EWSR1 and DDIT3, CLTC and ALK, ETV6 and PDGFRB, TPM3 and ALK, KMT2A and MLLT10, TMPRSS2 and ETV1, BRD4 and NUTM1, NUP98 and KDM5A, RANBP2 and ALK, CTNNB1 and PLAG1, KMT2A and ELL, TAF15 and NR4A3, FGFR3 and TACC3, PCM1 and JAK2, YWHAE and NUTM2B, STRN and ALK, CRTC3 and MAML2, CDH11 and USP6, CDKN2D and WDFY2, CIC and DUX4, SLC34A2 and ROS1, ATIC and ALK, CD74 and NRG1, MYB and NFIB, PRCC and TFE3, KIF5B and ALK, TMPRSS2 and ETV4, KMT2A and SEPT9, EWSR1 and POU5F1, FGFR1 and PLAG1, MN1 and ETV6, TBL1XR1 and TP63, KMT2A and EPS15, SLC45A3 and ELK4, DHH and RHEBL1, HEY1 and NCOA2, EZR and ROS1, GOPC and ROS1, HMGA2 and WIF1, KMT2A and CREBBP, SS18 and SSX4B, FAM131B and BRAF, EWSR1 and FEV, EWSR1 and PBX1, TPM4 and ALK, SND1 and BRAF, ACTB and GLI1, KMT2A and KNL1, KMT2A and SEPT6, SDC4 and ROS1, TFG and ALK, HNRNPA2B1 and ETV1, PTPRK and RSPO3, JAZF1 and PHF1, HMGA2 and RAD51B, KMT2A and MLLT11, TPR and NTRK1, AKAP9 and BRAF, FUS and CREB3L1, ETV6 and JAK2, HMGA2 and NFIB, KMT2A and AFF3, CHCHD7 and PLAG1, VTI1A and TCF7L2, LIFR and PLAG1, EWSR1 and ETV1, SRGAP3 and RAF1, KMT2A and AFF4, MEAF6 and PHF1, PAX3 and NCOA1, HAS2 and PLAG1, EWSR1 and NFATC2, HIP1 and ALK, GOLGA5 and RET, BCR and JAK2, EWSR1 and ETV4, DCTN1 and ALK, MBTD1 and CXorf67, NDRG1 and ERG, CARS and ALK, SFPQ and TFE3, KMT2A and ARHGAP26, KMT2A and EP300, KMT2A and TET1, PAX5 and JAK2, PPFIBP1 and ALK, YWHAE and NUTM2A, LRIG3 and ROS1, TFG and NTRK1, TPM3 and ROS1, SLC45A3 and ETV1, ERC1 and RET, SEC16A and NOTCH1, KTN1 and RET, SEC31A and JAK2, TCEA1 and PLAG1, QKI and NTRK2, RNF130 and BRAF, EIF3E and RSPO2, EWSR1 and ZNF444, LMNA and NTRK1, PPFIBP1 and ROS1, PWWP2A and ROS1, EWSR1 and YY1, FUS and ATF1, PAX3 and NCOA2, ZC3H7B and BOOR, BRD3 and NUTM1, CANT1 and ETV4, CIC and FOXO4, COL1A1 and USP6, EWSR1 and ZNF384, KMT2A and ABI1, KMT2A and ACTN4, KMT2A and CEP170B, KMT2A and FOXO3, KMT2A and GAS7, KMT2A and MLLT6, KMT2A and SEPT2, KMT2A and SEPT5, MSN and ALK, VCL and ALK, EZR and ERBB4, RELCH and RET, SLC3A2 and NRG1, TRIM24 and BRAF, KLC1 and ALK, ARID1A and MAST2, GPBP1L1 and MAST2, NFIX and MAST1, NOTCH1 and GABBR2, TADA2A and MAST1, ZNF700 and MAST1, TRIM24 and RET, TRIM33 and RET, SSBP2 and JAK2, KMT2A and EEFSEC, CLCN6 and BRAF, GNAI1 and BRAF, MKRN1 and BRAF, NACC2 and NTRK2, FGFR1 and TACC1, TRIM27 and RET, HMGA2 and FHIT, HOOK3 and RET, PCM1 and RET, CEP89 and BRAF, CLIP1 and ROS1, ERC1 and ROS1, HLA and A and ROS1, LSM14A and BRAF, MYO5A and ROS1, SHTN1 and ROS1, TP53 and NTRK1, TPM3 and ROS1, ZCCHC8 and ROS1, FGFR3 and BAIAP2L1, KLK2 and ETV1, ACSL3 and ETV1, NUP107 and LGR5, HMGA2 and CCNB1IP1, HMGA2 and COX6C, GATM and BRAF, HACL1 and RAF1, HERPUD1 and BRAF, ZSCAN30 and BRAF, SLC45A3 and BRAF, HMGA2 and LHFPL6, COL1A2 and PLAG1, ESRP1 and RAF1, IRF2BP2 and CDX1, TFG and NR4A3, CLTC and TFE3, EWSR1 and MYB, NONO and TFE3, FCHSD1 and BRAF, HMGA2 and EBF1, ACBD6 and RRP15, AGPAT5 and MCPH1, AGTRAP and BRAF, ARFIP1 and FHDC1, ATG4C and FBXO38, BBS9 and PKD1 L1, CENPK and KMT2A, CNBP and USP6, DDX5 and ETV4, EIF3K and CYP39A1, EPC1 and PHF1, ERO1A and FERMT2, ETV6 and ITPR2, EWSR1 and NFATC1, EWSR1 and PATZ1, EWSR1 and SMARCA5, EWSR1 and SP3, FBXL18 and RNF216, FGFR1 and ZNF703, FN1 and ALK, FUS and FEV, GMDS and PDE8B, HMGA2 and ALDH2, IL6R and ATP8B2, INTS4 and GAB2, JPT1 and USH1G, KLK2 and ETV4, KMT2A and ABI2, KMT2A and ARHGEF12, KMT2A and BTBD18, KMT2A and CASP8AP2, KMT2A and CBL, KMT2A and CIP2A, KMT2A and CT45A2, KMT2A and DAB2IP, KMT2A and FOXO4, KMT2A and FRYL, KMT2A and GMPS, KMT2A and GPHN, KMT2A and LASP1, KMT2A and LPP, KMT2A and MAPRE1, KMT2A and MYO1F, KMT2A and NCKIPSD, KMT2A and NRIP3, KMT2A and PDS5A, KMT2A and PICALM, KMT2A and PRRC1, KMT2A and SARNP, KMT2A and SH3GL1, KMT2A and SORBS2, KMT2A and TOP3A, KMT2A and ZFYVE19, MBOAT2 and PRKCE, MIA2 and GEMIN2, NF1 and ASIC2, NFIA and EHF, NTN1 and ACLY, OMD and USP6, PLA2R1 and RBMS1, PLXND1 and TMCC1, RAF1 and DAZL, RBM14 and PACS1, RGS22 and SYCP1, SEC31A and ALK, SEPT8 and AFF4, SLC22A1 and CUTA, SLC26A6 and PRKAR2A, SLC45A3 and ETV5, SQSTM1 and ALK, SS18L1 and SSX1, SSH2 and SUZ12, SUSD1 and PTBP3, TCF12 and NR4A3, TECTA and TBCEL, THRAP3 and USP6, TMPRSS2 and ETV5, TPR and ALK, UBE2L3 and KRAS, WDCP and ALK, SS18 and USP6


In some embodiments of the methods of the disclosure, a subject of the disclosure has been diagnosed with the disease or disorder. In some embodiments, the subject of the disclosure presents at least one sign or symptom of the disease or disorder. In some embodiments, the subject has a biomarker predictive of a risk of developing the disease or disorder. In some embodiments, the biomarker is a genetic mutation.


In some embodiments of the methods of the disclosure, a subject of the disclosure is female. In some embodiments of the methods of the disclosure, a subject of the disclosure is male. In some embodiments, a subject of the disclosure has two XX or XY chromosomes. In some embodiments, a subject of the disclosure has two XX or XY chromosomes and a third chromosome, either an X or a Y.


In some embodiments of the methods of the disclosure, a subject of the disclosure is a neonate, an infant, a child, an adult, a senior adult, or an elderly adult. In some embodiments of the methods of the disclosure, a subject of the disclosure is at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or 31 days old. In some embodiments of the methods of the disclosure, a subject of the disclosure is at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 or 12 months old. In some embodiments of the methods of the disclosure, a subject of the disclosure is at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100 or any number of years or partial years in between of age.


In some embodiments of the methods of the disclosure, a subject of the disclosure is a mammal. In some embodiments, a subject of the disclosure is a non-human mammal.


In some embodiments of the methods of the disclosure, a subject of the disclosure is a human.


In some embodiments of the methods of the disclosure, a therapeutically effective amount comprises a single dose of a composition of the disclosure. In some embodiments, a therapeutically effective amount comprises a therapeutically effective amount comprises at least one dose of a composition of the disclosure. In some embodiments, a therapeutically effective amount comprises a therapeutically effective amount comprises one or more dose(s) of a composition of the disclosure.


In some embodiments of the methods of the disclosure, a therapeutically effective amount eliminates a sign or symptom of the disease or disorder. In some embodiments, a therapeutically effective amount reduces a severity of a sign or symptom of the disease or disorder.


In some embodiments of the methods of the disclosure, a therapeutically effective amount eliminates the disease or disorder.


In some embodiments of the methods of the disclosure, a therapeutically effective amount prevents an onset of a disease or disorder. In some embodiments, a therapeutically effective amount delays the onset of a disease or disorder. In some embodiments, a therapeutically effective amount reduces the severity of a sign or symptom of the disease or disorder. In some embodiments, a therapeutically effective amount improves a prognosis for the subject.


In some embodiments of the methods of the disclosure, a composition of the disclosure is administered to the subject systemically. In some embodiments, the composition of the disclosure is administered to the subject by an intravenous route. In some embodiments, the composition of the disclosure is administered to the subject by an injection or an infusion.


In some embodiments of the methods of the disclosure, a composition of the disclosure is administered to the subject locally. In some embodiments, the composition of the disclosure is administered to the subject by an intraosseous, intraocular, intracerebrospinal or intraspinal route. In some embodiments, the composition of the disclosure is administered directly to the cerebral spinal fluid of the central nervous system. In some embodiments, the composition of the disclosure is administered directly to a tissue or fluid of the eye and does not have bioavailability outside of ocular structures. In some embodiments, the composition of the disclosure is administered to the subject by an injection or an infusion.


In some embodiments, the compositions comprising the trans-splicing RNAs disclosed herein are formulated as pharmaceutical compositions. Briefly, pharmaceutical compositions for use as disclosed herein may comprise a fusion protein(s) or a polynucleotide encoding the fusion protein(s), optionally comprised in an AAV, which is optionally also immune orthogonal, in combination with one or more pharmaceutically or physiologically acceptable carriers, diluents or excipients. Such compositions may comprise buffers such as neutral buffered saline, phosphate buffered saline and the like; carbohydrates such as glucose, mannose, sucrose or dextrans, mannitol; proteins; polypeptides or amino acids such as glycine; antioxidants; chelating agents such as EDTA or glutathione; adjuvants (e.g., aluminum hydroxide); and preservatives. Compositions of the disclosure may be formulated for oral, intravenous, topical, enteral, intraocular, and/or parenteral administration. In certain embodiments, the compositions of the present disclosure are formulated for intravenous administration.


EXAMPLE EMBODIMENTS

Embodiment 1: A trans-splicing nucleic acid, comprising: (a) one or more replacement domains that encode a therapeutic sequence operably linked to; (b) one or more intronic domains that promote RNA splicing of the replacement domain primarily in a specific tissue or cell type; and (c) one or more antisense domains that promote binding to a target RNA molecule.


Embodiment 2: The composition of embodiment 1, wherein the intronic domains promote trans-splicing primarily in brain tissue and are derived or isolated from genes selected from the group consisting of: UBB, GFAP, STMN1, RPL13A, UCHL1, TUBB2B, ALDOA, LRRC75A-AS1, DYNLRB1, TPI1, PKM, NDRG2, MT3, RTN3, SKP1, RTN1, NRGN, DBNDD2, BSCL2, ALDOC, S100B, GNG3, FXYD6, PRDX1, HSP90AA1, SRP14, ATF4, NDUFB8, CAPZB, ATF5, ADD1, ERGIC3, GUK1, DCTN1, GSN, MORF4L1, GNAI2, SPP1, CRIP2, RNASE1, EIF4G2, PAQR6, DCTN3, CALB2, COMMD6, MEST, STXBP1, CLDND1, SH3GLB2, NREP, ABCA2, SUN2, UBXN6, DBI, CDK2AP1, NFIX, SF1, HNRNPF, GPM6B, SQSTM1, MOBP, ANAPC11, POLD2, SAMD4B, IST1, TPD52, SHD, STK25, ABR, PQBP1, PHB, SEPT9, S100A13, APBB1, RANBP1, ARHGEF2, DLGAP4, GNAO1, SEZ6L2, FDFT1, CBX1, EIF4G1, FAM213A, MAPK8IP1, PSME1, CCAR2, TKT, SYN1, OGDH, OCIAD1, SLC3A2, SLC1A5, PTTG1, DNM1, MAP6, KDM1A, PTN, FN1, CSNK1E, TMEM9, MATR3, AHCYL1, ATP6V0A1, CDIP1, CYFIP2, SLIRP, SCG5, TPX2, DMTN, SSBP1, PFKL, KIAA0930, CAMTA1, PRC1, CHTF8, TMPRSS3, BIN1, CCT8, AMZ2, TM7SF2, SEC11A, CLPTM1, AMPD2, DHX9, GAB2, CLTC, PSME3, RPP21, DYNC1I2, CDC20, NOSIP, ATP13A2, AIF1L, SURF4, PTPRN2, STK24, SDR39U1, HMOX2, UBAP2L, NRCAM, CCDC3, GPT2, TN P02, NRXN2, PPP1R7, CAMK2A, UBE2L6, DBNL, ADAR, RNF220, MCM2, DTNA, PDIA6, PSMG2, URM1, SNX17, SV2A, SPG21, REEP2, HSD17B4, GSTO1, TRAPPC3, ZNF385A, SHISA5, FAM107B, MID1IP1, SERPINE2, CDK5RAP3, COMMD4, ANAPC15, CABP1, PTPRA, NPTN, RERE, VPS29, SPTBN2, MT1G, DTX3, GOLGA7, TPD52L2, EPB41L3, SPIN1, FARP1, NUP62, CNOT1, TMEM108, DUT, MAPRE2, PSD, NEBL, UBE2L3, KIF5C, TCF19, WDR54, CPSF7, GIT1, L1CAM, ITPR3, RHPN2, HMGA1, KCNAB2, HID1, SHF, CD99L2, C19ORF25, PAF1, B4GALT2, PTGES2, CAPG, SEMA4D, TTYH2, EXOC7, CNDP1, TACC2, BACE1, GABBR1, LMNB1, SLAIN1, GLG1, APBA2, CADM3, PC, PHLDB1, HHATL, SOGA1, CNTNAP2, RFC2, MAPK8IP3, CD200, REC8, TACC3, SYT13, SRP68, KLC4, CORO1C, ATPAF1, NARF, AP3B2, NUP93, FAM102A, SCN1B, NDUFS2, CBFA2T2, PSMA5, EPB41, and BCAR1.


Embodiment 3: The composition of embodiment 2, wherein the intronic domains promote trans-splicing primarily in brain tissue and are selected from the group consisting of SEQ ID NO 1-SEQ ID NO 492.


Embodiment 4: The composition of embodiments 2 or 3, wherein the replacement domain is derived or isolated from a human gene a selected from the group consisting of: SOD1, TARDBP, FUS, MATR3, SOD1, C9ORF72 (Amyotrophic lateral sclerosis); MAPT, PGRN (Frontotemporal dementia with parkinsonism); CDH23, MYO7A, USH2A (Usher's syndrome); GALC (Krabbe disease); SMPD1, NPC1, NPC2 (Niemann Pick disease); PRNP (prion disease); SCN1A (Dravet syndrome); PINK1, ATPGAP2 (early-onset Parkinson's disease); ATXN1, ATXN2, ATXN3, PLEKHG4, SPTBN2, CACNA1A, ATXN7, TTBK2, PPP2R2B, KCNC3, PRKCG, ITRP1, TBP, KCND1, FGF14 (spinocerebellar ataxias); SCN1A, SCN2A, CACNA1A, GRIN2B, GRIN2A, MECP2, FOXG1, SLC6A1, PRRT2, PTEN, KCNQ2, KCNQ3, STARD7, CLRN1 (genetic epilepsy disorders); ATM (Ataxia-telangiectasia); GLB1 (GM1 gangliosidosis); GBA (Gaucher disease); GM2A (GM2 gangliosidosis); UBE3A (Angelman syndrome); SLC2A1 (glucose transporter deficiency type 1); GLA (Fabry disease); MYOC, OPTN, TBK1, WDR36, CYPIB1 (Open Angle Glaucoma); IDUA (Hurler syndrome or Mucopolysaccharidosis 1); IDS (Hunter syndrome or Mucopolysaccharidosis 2); and CLN3 (Batten disease).


Embodiment 5: The composition of any one of embodiments 2-4, wherein the antisense domain is complementary to sequences derived or isolated from a human gene selected from the group consisting of: OD1, TARDBP, FUS, MATR3, SOD1, C9ORF72 (Amyotrophic lateral sclerosis); MAPT, PGRN (Frontotemporal dementia with parkinsonism); CDH23, MYO7A, USH2A (Usher's syndrome); GALC (Krabbe disease); SMPD1, NPC1, NPC2 (Niemann Pick disease); PRNP (prion disease); SCN1A (Dravet syndrome); PINK1, ATPGAP2 (early-onset Parkinson's disease); ATXN1, ATXN2, ATXN3, PLEKHG4, SPTBN2, CACNA1A, ATXN7, TTBK2, PPP2R2B, KCNC3, PRKCG, ITRP1, TBP, KCND1, FGF14 (spinocerebellar ataxics); SCN1A, SCN2A, CACNA1A, GRIN2B, GRIN2A, MECP2, FOXG1, SLC6A1, PRRT2, PTEN, KCNQ2, KCNQ3, STARD7, CLRN1 (genetic epilepsy disorders); ATM (Ataxia-telangiectasia); GLB1 (GM1 gangliosidosis); GBA (Gaucher disease); GM2A (GM2 gangliosidosis); UBE3A (Angelman syndrome); SLC2A1 (glucose transporter deficiency type 1); GLA (Fabry disease); MYOC, OPTN, TBK1, WDR36, CYPIB1 (Open Angle Glaucoma); IDUA (Hurler syndrome or Mucopolysaccharidosis 1); IDS (Hunter syndrome or Mucopolysaccharidosis 2); and CLN3 (Batten disease).


Embodiment 6: The composition of embodiment 1, wherein the intronic domains promote trans-splicing primarily in liver tissue and are derived or isolated from genes selected from the group consisting of: ORM1, ORM2, PLG, ALDOB, CYP2C8, CPS1, ADH4, PON1, CFHR3, C6, DBI, ITIH3, ITIH4, C4BPB, PTGR1, C5, LBP, PRDX1, HPD, UBB, HPR, ADH6, RPL15, CXCL2, DPYS, HAL, SLC39A14, AQP9, SERPINA10, ADH1A, HABP2, SERPINF2, ECI2, APOM, KLKB1, ACSM5, SLCO1B3, ASGR2, IL1RAP, PSMA1, GRB14, LRRC75A-AS1, BCHE, EPHX1, DMGDH, MST1, ACSM3, SEPT2, TFR2, NDUFC1, NR1H4, AFMID, HNMT, TTC39C, RDH16, GPAM, SLC13A5, SERF2, PICALM, PCSK6, TOM1L1, ANPEP, EBNA1BP2, RCL1, DEPDC7, BTG1, KRT8, ABCA6, GJB1, KMO, HSD17B13, LEAP2, PECR, LEPR, CTNND1, ENSA, MASP2, KYNU, ABCB4, DNASE1L3, ACSL5, UBE2V1, SPTBN1, RBPMS, RAB13, IL1R1, SERPINA5, FGGY, TMEM220, ACSS2, VNN1, CTAGE5, MS4A6A, MFSD2A, SKAP1, VDAC2, SLC22A7, PPP2R5C, SORBS2, CDV3, CSAD, TRPM8, TOMM5, SLCO2B1, MICU1, NCOR1, GSAP, CBWD5, ENPP1, SPATS2L, FKBP5, NR3C1, IGF2, IGF1, PLSCR4, MYLK, SLC27A5, ALDH5A1, TXLNGY, PSMB5, MOCOS, PET100, SGK1, AZI2, TJP2, FMO4, STEAP1, DMD, RNF14, RBM34, PABPC1L, ZCCHC9, ANO6, AGMAT, MASP1, IL17RB, MRPS27, MLIP, MON2, ETF1, SULT1B1, GBP7, PRKACA, PI4K2B, SP100, TMED6, NFU1, RMDN2, IP6K2, CCNB1IP1, PCBP1-AS1, SLC39A1, and PHF11


Embodiment 7: The composition of embodiment 6, wherein the intronic domains promote trans-splicing primarily in liver tissue and are selected from the group consisting of SEQ ID NO 493-SEQ ID NO 798.


Embodiment 8: The composition of any one of embodiments 6-7, wherein the replacement domain is derived or isolated from a human gene a selected from the group consisting of: GLB1 (GM1 gangliosidosis); GBA (Gaucher disease); GM2A (GM2 gangliosidosis); PCSK9, LDLR, APOB, APOE (Familial hypercholesterolemia); GAA (Pompe disease); MYOC, OPTN, TBK1, WDR36, CYPIB1 (Open Angle Glaucoma); IDS (Hunter syndrome or Mucopolysaccharidosis 2); IDUA (Hurler syndrome or Mucopolysaccharidosis 1); CLN3 (Batten disease); F9 (Hemophilia B); and F8 (Hemophilia A).


Embodiment 9: The composition of any one of embodiments 6-8, wherein the antisense domain is complementary to sequences derived or isolated from a human gene selected from the group consisting of: GLB1 (GM1 gangliosidosis); GBA (Gaucher disease); GM2A (GM2 gangliosidosis); PCSK9, LDLR, APOB, APOE (Familial hypercholesterolemia); GAA (Pompe disease); MYOC, OPTN, TBK1, WDR36, CYPIB1 (Open Angle Glaucoma); IDS (Hunter syndrome or Mucopolysaccharidosis 2); IDUA (Hurler syndrome or Mucopolysaccharidosis 1); CLN3 (Batten disease); F9 (Hemophilia B); and F8 (Hemophilia A).


Embodiment 10: The composition of embodiment 1, wherein the intronic domains promote trans-splicing primarily in heart tissue and are derived or isolated from genes selected from the group consisting of: TNNI3, TNNT2, MB, FHL2, CSRP3, FHL1, UBB, MYOM2, CD36, GSN, PDHA1, CRIP2, POPDC2, SORBS2, CPT1B, RYR2, ANXA11, ITGA7, SORBS1, NDUFC1, NEXN, MYH7B, ABLIM1, NEBL, CAST, NME2, CYB5R3, TRDN, TACC2, NDUFA5, MYL12B, FHOD3, CALCOCO2, NCAM1, GPD1L, SGCA, AQP1, AKAP1, ARL6IP4, LRRC39, TTN, TSC22D1, PPP1R12B, OBSL1, MYO15B, PKIG, HMOX2, CDK18, BTNL9, ITGB1BP2, SEPT2, CAV2, DTNA, C15orf41, ATP1A1, BCO2, MYL4, ADCY5, CLIC5, SLC8A1, RRAD, AKAP13, AK3, MYO1C, FGF12, LAMA5, ADAM15, WNK2, SUN1, KLHL7, RNF207, HIF3A, HSPG2, LPCAT4, ADH1B, ENY2, RGS3, FRMD3, CCNB1IP1, PHC2, STAB1, HAND2-AS1, PTPN3, PARVB, PKD1, SPEG, SYNE2, ZNF638, SEPT4, DAG1, SLC12A7, FBXO18, FAM107A, RBM17, MYPN, GRIP2, SCN5A, WFS1, RABGAP1L, SYNE1, HLA-DRB5, SNRPN, MEG3, RAPGEF3, SEC13, C1QTNF1, UCKL1, FRY, IP6K2, CUX1, ANK3, MYH14, ADCY4, C3orf18, MYLK3, PXN, TSPAN18, PDE1C, C6orf48, BCS1L, ATG4D, HRAS, TENM2, PLXNB1, N4BP2L2, TNXB, LTBP1, MAPK8IP3, COL1A1, GOAT, ACOT11, MTUS2, VPS45, and KCNIP2.


Embodiment 11: The composition of embodiment 10, wherein the intronic domains promote trans-splicing primarily in heart tissue and are selected from the group consisting of SEQ ID NO 799-SEQ ID NO 1104.


Embodiment 12: The composition of embodiments 10 or 11, wherein the replacement domain is derived or isolated from a human gene a selected from the group consisting of: LAMP2 (Danon disease); GLA (Fabry disease); SLC2A1 (glucose transporter deficiency type 1); UBE3A (Angelman syndrome); MYOC, OPTN, TBK1, WDR36, CYPIB1 (Open Angle Glaucoma); IDUA (Hurler syndrome or Mucopolysaccharidosis 1); IDS (Hunter syndrome or Mucopolysaccharidosis 2); CLN3 (Batten disease); LMNA (Limb-girdle muscular dystrophy type 1B); DMD (Duchenne muscular dystrophy); DYSF (Limb-girdle muscular dystrophy type 2B); SGCB (Limb-girdle muscular dystrophy type 2E); SGCG (Limb-girdle muscular dystrophy type 2C); SGCA (Limb-girdle muscular dystrophy type 2D); SGCD (Limb-girdle muscular dystrophy type 2F); DUX4, D4Z4 (Facioscapulohumeral muscular dystrophy); F9 (Hemophilia B); F8 (Hemophilia A).


Embodiment 13: The composition of any one of embodiments 10-12, wherein the antisense domain is complementary to sequences derived or isolated from a human gene selected from the group consisting of: LAMP2 (Danon disease); GLA (Fabry disease); SLC2A1 (glucose transporter deficiency type 1); UBE3A (Angelman syndrome); MYOC, OPTN, TBK1, WDR36, CYPIB1 (Open Angle Glaucoma); IDUA (Hurler syndrome or Mucopolysaccharidosis 1); IDS (Hunter syndrome or Mucopolysaccharidosis 2); CLN3 (Batten disease); LMNA (Limb-girdle muscular dystrophy type 1B); DMD (Duchenne muscular dystrophy); DYSF (Limb-girdle muscular dystrophy type 2B); SGCB (Limb-girdle muscular dystrophy type 2E); SGCG (Limb-girdle muscular dystrophy type 2C); SGCA (Limb-girdle muscular dystrophy type 2D); SGCD (Limb-girdle muscular dystrophy type 2F); DUX4, D4Z4 (Facioscapulohumeral muscular dystrophy); F9 (Hemophilia B); F8 (Hemophilia A).


Embodiment 14: The composition of embodiment 1, wherein the intronic domains promote trans-splicing primarily in retinal tissue and are derived or isolated from genes selected from the group consisting of: ADGRV1, AMPH, ATP1B2, BSG, CASK, CC2D2A, CEP290, CLTC, DMD, DOC2B, EFR3A, ERP44, FARP2, FN3KRP, GRAMD1B, GSS, HCN1, IFT140, IMPDH1, INTS13, KCTD5, KIF1B, KIF1B, KMT2D, MADD, MAN2A2, MARCH1, MSI2, NAP1L4, PPP3CC, PROM1, PTPRF, PTPRF, RIMS2, SLC17A7, SLC4A7, STXBP5, TTC8, UNC13B, UNC13B, WDR47, and YKT6.


Embodiment 15: The composition of embodiment 14, wherein the replacement domain is derived or isolated from a human gene a selected from the group consisting of: USHA2A, RPGR, RP2, RHO, PRPF31, USH1F, PRPF3, PRPF6 (Retinitis pigmentosa)


Embodiment 16: The composition of embodiments 14 or 15, wherein the antisense domain is complementary to sequences derived or isolated from a human gene selected from the group consisting of: USHA2A, RPGR, RP2, RHO, PRPF31, USH1F, PRPF3, PRPF6 (Retinitis pigmentosa)


Embodiment 17: The composition of embodiment 1, wherein the intronic domains promote trans-splicing primarily in muscle tissue and are derived or isolated from genes selected from the group consisting of: ALDOA, TNNT3, MYBPC1, ATP2A1, NEB, STAC3, CMYA5, MYH7, TNNI1, CA3, ASB5, PLIN2, TRDN, OBSCN, RYR1, RAP1A, SPARCL1, CSNK1A1, NPEPPS, AKR1C3, IDI2, MBNL1, FNDC5, TMOD4, ATP6V1F, DAP3, KPNB1, NOMO2, BEST3, HP1BP3, SEPT2, TIMM23, MYPN, DHRS7C, PALLD, POLR2J3, KANSL3, THOC7, MEF2C, NOMO1, YWHAZ, ZBTB16, SPAG9, CCZ1, MYLK2, ASAH1, GTF2I, CCPG1, SURF4, SMARCA2, CLTCL1, SLC6A6, CHRNB1, MAFG, COMMD6, SLC7A8, CDK11B, and DNASE1L1


Embodiment 18: The composition of embodiment 17, wherein the intronic domains promote trans-splicing primarily in muscle tissue and are selected from the group consisting of SEQ ID NO 1105-SEQ ID NO 1220.


Embodiment 19: The composition of embodiments 17 or 18, wherein the replacement domain is derived or isolated from a human gene a selected from the group consisting of: LAMP2 (Danon disease); GLA (Fabry disease); SLC2A1 (glucose transporter deficiency type 1); UBE3A (Angelman syndrome); MYOC, OPTN, TBK1, WDR36, CYPIB1 (Open Angle Glaucoma); IDUA (Hurler syndrome or Mucopolysaccharidosis 1); IDS (Hunter syndrome or Mucopolysaccharidosis 2); CLN3 (Batten disease); LMNA (Limb-girdle muscular dystrophy type 1B); DMD (Duchenne muscular dystrophy); DYSF (Limb-girdle muscular dystrophy type 2B); SGCB (Limb-girdle muscular dystrophy type 2E); SGCG (Limb-girdle muscular dystrophy type 2C); SGCA (Limb-girdle muscular dystrophy type 2D); SGCD (Limb-girdle muscular dystrophy type 2F); DUX4, D4Z4 (Facioscapulohumeral muscular dystrophy); F9 (Hemophilia B); and F8 (Hemophilia A)


Embodiment 20: The composition of any one of embodiments 17-19, wherein the antisense domain is complementary to sequences derived or isolated from a human gene selected from the group consisting of: LAMP2 (Danon disease); GLA (Fabry disease); SLC2A1 (glucose transporter deficiency type 1); UBE3A (Angelman syndrome); MYOC, OPTN, TBK1, WDR36, CYPIB1 (Open Angle Glaucoma); IDUA (Hurler syndrome or Mucopolysaccharidosis 1); IDS (Hunter syndrome or Mucopolysaccharidosis 2); CLN3 (Batten disease); LMNA (Limb-girdle muscular dystrophy type 1B); DMD (Duchenne muscular dystrophy); DYSF (Limb-girdle muscular dystrophy type 2B); SGCB (Limb-girdle muscular dystrophy type 2E); SGCG (Limb-girdle muscular dystrophy type 2C); SGCA (Limb-girdle muscular dystrophy type 2D); SGCD (Limb-girdle muscular dystrophy type 2F); DUX4, D4Z4 (Facioscapulohumeral muscular dystrophy); F9 (Hemophilia B); and F8 (Hemophilia A)


Embodiment 21: The composition of embodiment 1, wherein the the intronic domains carry binding sites that are preferentially-targeted by RNA-binding proteins with disease-causing mutations.


Embodiment 22: The composition of any one of embodiments 1-21, wherein the intronic domains carry binding sites for RNA-binding proteins selected from the group consisting of: DDX39, DDX3X, DDX3Y, DDX41, DDX5, DHX16, DHX8, DHX9, INTS6, DDX48, HNRNPA0, HNRNPA1, HNRNPA2B1, HNRPA3, HNRPAB, HNRPC, HNRPDL, HNRPF, HNRPH1, HNRPH2, HNRPLL, HNRPM, HNRPUL1, PTBP1, PTBP2, RBMXL2, SYNCRIP, CLK3, CLK4, SRPK1, SRPK2, SRPK3, ARS2, BUB3, C7ORF30, CIRBP, CRNKL1, CSDA, DNAJC8, EEF1A1, EIF3S10, FMR1, GRSF1, GTL3, IGF2BP3, IK, ILF2, ILF3, MATR3, MBNL1, MBNL2, MBNL3, MOV10, MSI1, MSI2, MYEF2, NHP2L1, PABPC1, PCBP1, PCBP2, PCBP4, PPIE, PPIL2, PPIL2, PPM1G, PPWD1, PRPF6, PSEN1, RALY, RBM15, RBM9, RBMS1, RBMX, RDBP, ROD1, SSB, THOC3, THOC4, TNPO1, TOP1MT, ZFP207, EFTUD2, LSM1, LSM2, LSM3, LSM4, LSM7, LSM8, NA, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SNRPA, SNRPA1, SNRPB, SNRPC, SNRPD1, SNRPD2, SNRPG, SNRPN, TXNL4, U2AF1L1, U2AF1L2, USP39, WDR57, PRPF31, PRPF4, PRPF40A, RBM17, NA, A2BP1, ACIN1, ASCC3L1, BCAS2, BRUNOL4, BUD13, BUD31, CD2BP2, CDC40, CDC5L, CRKRS, CUGBP2, ELAVL1, ELAVL1, ELAVL1, ELAVL2, ELAVL3, ELAVL4, FUSIP1, HSPA1B, HSPA5, HSPA8, NCBP2, NONO, NOVA1, NUDT21, PLRG1, PRPF19, PSIP1, RBM8A, RBMS1, SMU1, SNW1, TCERG1, TIA1, TIAL1, TNRC4, WBP11, WTAP, XAB2, YBX1, SFRS1, SFRS10, SFRS16, SFRS3, SFRS4, and SFRS6


Embodiment 23: The composition of any one of embodiments 1-22, wherein the trans-splicing RNA comprises an untranslated region that increases that alters the processing of the trans-splicing nucleic acid.


Embodiment 24: the composition of any one of embodiments 1-23, wherein the sequence comprising the trans-splicing nucleic acid comprises a sequence that is bound by an RNA-binding protein that increases the trans-splicing efficiency.


Embodiment 25: the composition of any of one embodiments 1-24, wherein the trans-splicing nucleic acid is RNA, DNA, a DNA/RNA hybrid, nucleic acid analog, a chemically-modified nucleic acid, or a chimera composed of two or more nucleic acids or nucleic acid analogs.


Embodiment 26: the composition of any of one embodiments 1-25, wherein the wherein the trans-splicing nucleic acid molecule further comprises a heterologous promoter.


Embodiment 27: the composition of any of one embodiments 1-26, wherein the promoter is isolated or derived from a promoter capable of driving expression of a transfer RNA (tRNA).


EXAMPLES
Example 1: Methods for Identification of Intronic Domains that Promote Trans-Splicing in Muscle

In order to identify Intronic Domains that will promote trans-splicing in muscle but not other tissues, the following experimental strategy is conducted: 1) analysis of unbiased RNA sequencing data from muscle, brain, liver, and kidney, and 2) identification of exons that are included primarily in muscle (“muscle-specific exons”), then 3) extraction of intronic sequences that are adjacent to the muscle-specific exons (“muscle-specific introns”). These muscle-specific introns promote inclusion of adjacent exons during RNA splicing, but RNA trans-splicing is less-efficient and most intronic sequences do not function efficiently in the context of trans-splicing. In order to identify muscle-specific introns that function in the context of RNA trans-splicing (“muscle-specific trans-splicing introns” or MSTI's), candidate MSTI's are selected from the group of muscle-specific introns and compared in a trans-splicing tissue culture experiment. The readout for the trans-splicing tissue culture experiment relies on a reporter that carries a GFP coding sequence interrupted by an exon carrying a stop codon and flanked by two introns (FIG. 4A). When transfected into a mammalian cell line, this reporter does not produce active GFP protein (FIG. 4B). The candidate MSTI's are placed in a trans-splicing RNA vector carrying antisense sequences that target the reporter and an exon that contains no stop codon (FIG. 4A). Successful trans-splicing among the trans-splicing vector and the reporter results in production of active GFP protein (FIG. 4B). In this manner, GFP expression serves as a proxy for the efficiency associated with RNA trans-splicing afforded by the MSTI. GFP expression among experiments conducted in muscle cell lines compared to brain, liver, and kidney cell lines or primary cells is assessed. MSTI's will be identified based on the following criteria: both 1) high GFP expression in muscle cell lines or primary cells, and 2) low GFP expression in brain, liver, and kidney cell lines or primary cells. These MSTI's serve as Intronic Domains that promote tissue-specific trans-splicing in muscle but not other tissues.


Example 2: Identification of Intronic Domains that Promote Trans-Splicing in Brain, Liver, or Kidney Tissue

In order to identify Intronic Domains that will promote trans-splicing in other tissues such as brain, liver, or kidney, a similar experiment as above is conducted. Here, introns that promote tissue-specific exon inclusion in the respective tissue will be compared in a similar assay. The candidate tissue-specific intronic sequences are similarly placed in a trans-splicing RNA vector and successful tissue-specific intronic sequences are identified based upon the following criteria: both 1) high GFP expression in cells derived from the chosen cell lines or primary cells, and 2) low GFP expression in cell lines or primary cells derived from other tissues. Intronic sequences that fit these criteria will serve as Intronic Domains that promote tissue-specific trans-splicing in a chosen tissue but not other tissues.


Example 3: Identification of a Therapeutic Trans-Splicing System to Treat Limb-Girdle Muscular Dystrophy (LGMD) that is Active Exclusively in Muscle Tissue

LGMD1B is a devastating muscle disease that causes progressive muscle wasting and death. The disease is caused by mutations in the gene LMNA that cause dysfunctional behavior of the gene (specifically, inappropriate inclusion of exon 9 which carries a stop codon). In order to identify trans-splicing systems that address this dysfunctional inclusion of exon 9 in LMNA in order to treat LGMD1B, we compare tens of systems that replace exon 9 with a short exon that carries no stop codons. We constructs 50+ trans-splicing RNA constructs that carry introns that are effectively spliced in muscle but not other tissues (SEQ ID NO 1105-SEQ ID NO 1220) and carry a replacement a reporter that carries a replacement for exon 9 from LMNA gene (FIG. 5A). Next, we Then, we co-transfect this reporter and individual trans-splicing RNA constructs in human cells lines derived from muscle or another non-muscle cell-type such as HEK cells. Next, we conduct RT-PCR measurements in both cell types primers flanking exon 9 to identify degree of replacement of exon 9 by the trans-splicing RNA replacement sequence. Finally, high-quality trans-splicing RNA systems are identified by the following metric: 1) high levels of replacement sequence inclusion in the LMNA reporter in muscle cells (>50%), with 2) low levels of replacement sequence inclusion in the LMNA reporter in HEK cells (<20%).


Cells and Tissues

In some embodiments of the compositions and methods of the disclosure, a cell of the disclosure is a eukaryotic cell. In some embodiments, the cell is a mammalian cell. In some embodiments, the cell is a bovine, murine, feline, equine, porcine, canine, simian, or human cell. In some embodiments, the cell is a non-human mammalian cell such as a non-human primate cell. In some embodiments, a cell of the disclosure is a somatic cell. In some embodiments, a cell of the disclosure is a germline cell. In some embodiments, a germline cell of the disclosure is not a human cell.


In some embodiments of the compositions and methods of the disclosure, a cell of the disclosure is a stem cell. In some embodiments, a cell of the disclosure is an embryonic stem cell. In some embodiments, an embryonic stem cell of the disclosure is not a human cell. In some embodiments, a cell of the disclosure is a multipotent stem cell or a pluripotent stem cell. In some embodiments, a cell of the disclosure is an adult stem cell. In some embodiments, a cell of the disclosure is an induced pluripotent stem cell (iPSC). In some embodiments, a cell of the disclosure is a hematopoietic stem cell (HSC).


In some embodiments of the compositions and methods of the disclosure, an immune cell of the disclosure is a lymphocyte. In some embodiments, an immune cell of the disclosure is a T lymphocyte (also referred to herein as a T-cell). Exemplary T-cells of the disclosure include, but are not limited to, naïve T cells, effector T cells, helper T cells, memory T cells, regulatory T cells (Tregs) and Gamma delta T cells. In some embodiments, an immune cell of the disclosure is a B lymphocyte. In some embodiments, an immune cell of the disclosure is a natural killer cell. In some embodiments, an immune cell of the disclosure is an antigen-presenting cell.


In some embodiments of the compositions and methods of the disclosure, a muscle cell of the disclosure is a myoblast or a myocyte. In some embodiments, a muscle cell of the disclosure is a cardiac muscle cell, skeletal muscle cell or smooth muscle cell. In some embodiments, a muscle cell of the disclosure is a striated cell.


In some embodiments of the compositions and methods of the disclosure, a somatic cell of the disclosure is an epithelial cell. In some embodiments, an epithelial cell of the disclosure forms a squamous cell epithelium, a cuboidal cell epithelium, a columnar cell epithelium, a stratified cell epithelium, a pseudostratified columnar cell epithelium or a transitional cell epithelium. In some embodiments, an epithelial cell of the disclosure forms a gland including, but not limited to, a pineal gland, a thymus gland, a pituitary gland, a thyroid gland, an adrenal gland, an apocrine gland, a holocrine gland, a merocrine gland, a serous gland, a mucous gland and a sebaceous gland. In some embodiments, an epithelial cell of the disclosure contacts an outer surface of an organ including, but not limited to, a lung, a spleen, a stomach, a pancreas, a bladder, an intestine, a kidney, a gallbladder, a liver, a larynx or a pharynx. In some embodiments, an epithelial cell of the disclosure contacts an outer surface of a blood vessel or a vein.


In some embodiments of the compositions and methods of the disclosure, a brain cell of the disclosure is a neuronal cell. In some embodiments, a neuron cell of the disclosure is a neuron of the central nervous system. In some embodiments, a neuron cell of the disclosure is a neuron of the brain or the spinal cord. In some embodiments, a neuron cell of the disclosure is a neuron of a cranial nerve or an optic nerve. In some embodiments, a neuron cell of the disclosure is a neuron of the peripheral nervous system. In some embodiments, a neuron cell of the disclosure is a neuroglial or a glial cell. In some embodiments, a glial of the disclosure is a glial cell of the central nervous system including, but not limited to, oligodendrocytes, astrocytes, ependymal cells, and microglia. In some embodiments, a glial of the disclosure is a glial cell of the peripheral nervous system including, but not limited to, Schwann cells and satellite cells.


In some embodiments of the compositions and methods of the disclosure, a liver cell of the disclosure is a hepatocytes. In some embodiments, a liver cell of the disclosure is a hepatic stellate cell. In some embodiments, a liver cell of the disclosure is Kupffer cell. In some embodiments, a liver cell of the disclosure is a sinusoidal endothelial cells.


In some embodiments of the compositions and methods of the disclosure, a retinal cell of the disclosure is a photoreceptor. In some embodiments, a photoreceptor cell of the disclosure is a rod. In some embodiments, a retinal cell of the disclosure is cone. In some embodiments, a retinal cell of the disclosure is a bipolar cell. In some embodiments, a retinal cell of the disclosure is a ganglion cell. In some embodiments, a retinal cell of the disclosure is a horizontal cell. In some embodiments, a retinal cell of the disclosure is an amacrine cell.


In some embodiments of the compositions and methods of the disclosure, a heart cell of the disclosure is a cardiomyocyte. In some embodiments, a heart cell of the disclosure is a cardiac pacemaker cell.


In some embodiments of the compositions and methods of the disclosure, a somatic cell of the disclosure is a primary cell.


In some embodiments of the compositions and methods of the disclosure, a somatic cell of the disclosure is a cultured cell.


In some embodiments of the compositions and methods of the disclosure, a somatic cell of the disclosure is in vivo, in vitro, ex vivo or in situ.


In some embodiments of the compositions and methods of the disclosure, a somatic cell of the disclosure is autologous or allogeneic.


INCORPORATION BY REFERENCE

Every document cited herein, including any cross referenced or related patent or application is hereby incorporated herein by reference in its entirety unless expressly excluded or otherwise limited. The citation of any document is not an admission that it is prior art with respect to any invention disclosed or embodimented herein or that it alone, or in any combination with any other reference or references, teaches, suggests or discloses any such invention. Further, to the extent that any meaning or definition of a term in this document conflicts with any meaning or definition of the same term in a document incorporated by reference, the meaning or definition assigned to that term in this document shall govern.


OTHER EMBODIMENTS

While particular embodiments of the disclosure have been illustrated and described, various other changes and modifications can be made without departing from the spirit and scope of the disclosure. The scope of the appended claims includes all such changes and modifications that are within the scope of this disclosure.

Claims
  • 1.-31. (canceled)
  • 32. A trans-splicing nucleic acid, comprising: (a) one or more replacement domains that encode a therapeutic sequence; (b) one or more intronic domains, wherein the one or more intronic domains promote ribonucleic acid (RNA) splicing of the replacement domain primarily in a specific tissue or cell type, and wherein the one or more intronic domains comprise one or more RNA-binding protein sites configured to promote tissue-specific splicing patterns; and (c) one or more antisense domains that promote binding to a target RNA molecule.
  • 33. The trans-splicing nucleic acid of claim 32, wherein the intronic domains comprise one or more sequences that are preferentially targeted by RNA-binding proteins carrying disease-associated mutations.
  • 34. The trans-splicing nucleic acid of claim 32, further comprising a 3′ untranslated region that improves a trans-splicing efficiency of the trans-splicing nucleic acid to the target RNA.
  • 35. The trans-splicing nucleic acid of claim 32, further comprising a 5′ untranslated region that improves the trans-splicing efficiency of the trans-splicing nucleic acid to the target RNA.
  • 36. The trans-splicing nucleic acid of claim 32, further comprising an RNA-binding protein that strengthens the interaction among the trans-splicing nucleic acid molecule and the target RNA molecule, thereby increasing a trans-splicing efficiency of the trans-splicing nucleic acid to the target RNA.
  • 37. The trans-splicing nucleic acid of claim 32, wherein the trans-splicing nucleic acid comprises RNA, DNA, a DNA/RNA hybrid, a nucleic acid analog, a chemically-modified nucleic acid, or a chimera composed of two or more nucleic acids or nucleic acid analogs.
  • 38. A method for modifying a target RNA molecule in a cell, the method comprising administering a trans-splicing nucleic acid to the cell, wherein the trans-splicing nucleic acid comprises (a) one or more replacement domains that encode a therapeutic sequence; (b) one or more intronic domains, wherein the one or more intronic domains promote ribonucleic acid (RNA) splicing of the replacement domain primarily in a specific tissue or cell type, and wherein the one or more intronic domains comprise one or more RNA-binding protein sites configured to promote tissue-specific splicing patterns; and (c) one or more antisense domains that promote binding to the target RNA molecule.
  • 39. The method of claim 38, wherein the one or more intronic domains comprise one or more sequences that are preferentially targeted by RNA-binding proteins carrying disease-associated mutations.
  • 40. The method of claim 38, wherein the trans-splicing nucleic acid further comprises a 3′ untranslated region that improves the trans-splicing efficiency of the trans-splicing nucleic acid to the target RNA.
  • 41. The method of claim 38, wherein the trans-splicing nucleic acid further comprises a 5′ untranslated region that improves the trans-splicing efficiency of the trans-splicing nucleic acid to the target RNA.
  • 42. The method of claim 38, further comprising providing an RNA-binding protein that strengthens the interaction among the trans-splicing nucleic acid and the target RNA molecule, and wherein a strengthening of the interaction among the trans-splicing nucleic acid molecule and the target RNA molecule increases a trans-splicing efficiency of the trans-splicing nucleic acid to the target RNA.
  • 43. The method of claim 38, wherein the trans-splicing nucleic acid comprises RNA, DNA, a DNA/RNA hybrid, a nucleic acid analog, a chemically-modified nucleic acid, or a chimera composed of two or more nucleic acids or nucleic acid analogs.
  • 44. The method of claim 38, further comprising inserting the therapeutic sequence into the target RNA.
  • 45. The method of claim 44, wherein the therapeutic sequence is not inserted into a target RNA in a second tissue or a second cell type.
  • 46. A trans-splicing nucleic acid, comprising: (a) one or more replacement domains that encode a therapeutic sequence; (b) one or more intronic domains configured to promote ribonucleic acid (RNA) splicing of the one or more replacement domains primarily to a target RNA in a specific tissue or a specific cell type, and wherein the one or more intronic domains comprise a sequence configured to be bound by an RNA-binding protein that increases a trans-splicing efficiency of the therapeutic sequence to the target RNA; and (c) one or more antisense domains that promote binding to a target RNA molecule.
  • 47. The trans-splicing nucleic acid of claim 46, wherein the one or more intronic domains comprise a sequence preferentially targeted by an RNA-binding protein carrying one or more disease-associated mutations.
  • 48. The trans-splicing nucleic acid of claim 46, further comprising a 3′ untranslated region that improves the trans-splicing efficiency of the therapeutic sequence to the target RNA.
  • 49. The trans-splicing nucleic acid of claim 46, further comprising a 5′ untranslated region that improves the trans-splicing efficiency of the therapeutic sequence to the target RNA.
  • 50. The trans-splicing nucleic acid of claim 46, further comprising the RNA-binding protein that increases the trans-splicing efficiency of the therapeutic sequence to the target RNA.
  • 51. The trans-splicing nucleic acid of claim 46, wherein the trans-splicing nucleic acid comprises RNA, DNA, a DNA/RNA hybrid, a nucleic acid analog, a chemically-modified nucleic acid, or a chimera composed of two or more nucleic acids or nucleic acid analogs.
CROSS-REFERENCE

This application is a continuation of International Application No. PCT/US2021/052315, filed on Sep. 28, 2021, which claims priority to U.S. provisional application No. 63/084,027, filed on Sep. 28, 2020, the entirety of which are hereby incorporated by reference herein.

Provisional Applications (1)
Number Date Country
63084027 Sep 2020 US
Continuations (1)
Number Date Country
Parent PCT/US2021/052315 Sep 2021 US
Child 18189896 US