Patent Application
20060041945
The present invention relates to the field of genetic engineering.
Gene targeting by homologous recombination is a powerful means to specifically modify a gene of interest. The availability of embryonic stem (ES) cells has been instrumental in the study of gene function in mice. In non-murine mammalian species, the lack of ES cells has been circumvented by gene targeting in primary somatic cells, followed by nuclear transfer. In spite of advances in molecular biology techniques, gene targeting in primary cells remains a challenge given the low frequency of homologous recombination (McCreath et al. (2000) Nature 405:1066-1069), the short lifespan of primary cells, and limitations in methods allowing the selection of properly targeted cells. Currently, primary cell gene targeting and production of offspring has mainly been performed with transcriptionally active genes, which are associated with a higher frequency of homologous recombination relative to silent genes (Denning et al. (2001) Nat. Biotechnol. 19:559-562). Furthermore, the selection of correctly targeted cells may be accomplished by having the targeted gene promoter drive the expression of a selection marker, a process which is not applicable to silent genes (Denning et al., supra; Lai et al. (2002) Science 295:1089-1092; Yifan et al. (2002) Nat. Biotechnol. 20:251-255; Thomson, A. J., et al., (2003) Reprod. Suppl. 61:495-508).
To fully evaluate the consequences of a genetic modification, both alleles of a gene need to be disrupted. In mice, this is generally accomplished by back crossing from a hemizygous transgenic founder animal to produce a homozygous targeted inbred line. Breeding to homozygosity is extremely time consuming in the mouse and represents an even more severe impediment in species that have a long generation interval and that are negatively impacted by the consequences of inbreeding. In the pig, two innovative approaches have been used to circumvent this limitation and produce homozygous β(1,3)-galactocyltransferase knockout animals. Hemizygous targeted primary cells were selected in vitro for the lack of the enzymatic activity resulting either from a spontaneous point mutation in the second allele of the gene (Denning et al., (2003) Reproduction 126:1-11) or for mitotic recombinants (Piedrahita (2000) Theriogenology 53: 105-16) and cloned offspring were made from the homozygous knockout cell lines. Unfortunately, these approaches are not generally useful for silent genes nor widely applicable for active genes. Thus, improved methods to study gene function in non-human mammals are desirable.
Antibody Production in Genetically Modified Animals
In 1890, Shibasaburo Kitazato and Emil Behring reported an experiment with extraordinary results; particularly, they demonstrated that immunity can be transferred from one animal to another by taking serum from an immune animal and injecting it into a non-immune one. This landmark experiment laid the foundation for the introduction of passive immunization into clinical practice. Today, the preparation and use of human immunoglobulin for passive immunization is standard medical practice. In the United States alone, there is a $1.4B per annum market for human immunoglobulin, and each year more than 16 metric tons of human antibody is used for intravenous antibody therapy. Comparable levels of consumption exist in the economies of most highly industrialized countries, and the demand can be expected to grow rapidly in developing countries. Currently, human antibody for passive immunization is obtained from the pooled serum of human donors. This means that there is an inherent limitation in the amount of human antibody available for therapeutic and prophylactic usage. Already, the demand exceeds the supply and severe shortfalls in availability have been routine.
In an effort to overcome some of the problems associated with the inadequate supply of human immunoglobulin, various technologies have been developed. For example, the production of human immunoglobulin by recombinant methods in tissue culture is routine. Particularly, the recombinant expression of human immunoglobulin in CHO expression systems is well known, and is currently utilized for the production of several human immunoglobulins and chimeric antibodies now in therapeutic use.
Mice retaining an unrearranged human immunoglobulin gene have also been developed for the production of human antibodies (e.g., monoclonal antibodies) (see, for example, PCT Publication Nos. WO98/24893; WO96/33735; WO 97/13852; WO98/24884; WO97/07671; and U.S. Pat. Nos. 5,877,397; 5,874,299; 5,814,318; 5,789,650; 5,770,429; 5,661,016; 5,633,425; 5,625,126; 5,569,825; and 5,545,806).
PCT Publication No. WO00/10383 describes modifying a human chromosome fragment and transferring the fragment into certain cells via microcell fusion. U.S. Pat. Nos. 5,849,992 and 5,827,690 describe the production of monoclonal antibodies in the milk of transgenic animals including mice, sheep, pigs, cows, and goats wherein the transgenic animals expressed human immunoglobulin genes under the control of promoters that provide for the expression of the antibodies in mammary epithelial cells. Essentially, this results in the expression of the antibodies in the milk of such animals, for example a cow. U.S. Patent Publication Nos. 2003-0037347-0 describe the expression of xenogenous human immunoglobulins in cloned, transgenic ungulates.
Notwithstanding the foregoing, further improved methods for producing ungulates that are amenable to being used as hosts for xenogenous antibody production are of great value to this industry.
Production of Prion Protein-Deficient Bovines
The cellular prion protein PrPC is a ubiquitously expressed, plasma membrane, glycosylphosphatidylinositol-anchored glycoprotein. This protein plays a crucial role in the pathogenesis of transmittable spongiform encephalopathies such as BSE, in cattle, and Creutzfeldt-Jakob disease (CJD), in humans. Its disease-associated, protease-resistant isoform, PrPSc, has the ability to convert normal PrPC to PrPSc and is considered essential for the pathogenesis and transmittance of spongiform encephalopathies. Although the accumulation of PrPSc in neurons is associated with fatal neurodegeneration, the normal physiological function of the protein remains unclear. Mice with disruptions restricted to the coding region of the PrP gene have been generated and show only minor phenotypic deficits. Importantly, they are resistant to infection by PrPSc, suggesting that PrPC is necessary for pathogenesis of prion diseases (Prusiner et al. (1993) Proc. Natl. Acad. Sci. USA 90:10608-10612; Weissmann et al. (1994) Ann. NY Acad. Sci. 724:235-240).
BSE was first recognized in 1986 in the United Kingdom and now has been spread to many countries throughout the world. BSE can be transmitted from cattle to humans by direct consumption of contaminated beef products, resulting in a variant form of CJD (vCJD). Currently, there is no cure for the fatal disease. To reduce risk of exposure to the disease-causing PrPSc protein, expensive testing programs have been implemented and efforts to remove bovine components from a wide variety of products have been initiated in many countries. Cattle with homozygous null mutation in the PrP gene alleles could be used to alleviate concerns about BSE-contaminated bovine products. Furthermore, these animals could be useful as a model, in addition to the mouse, for investigating the involvement of PrP in BSE.
In general, the invention features genetically modified non-human mammals (e.g., bovines and other ungulates), and methods of making these mammals. In particular, the invention features transgenic ungulates having reduced levels of endogenous IgM heavy chain and/or prion protein.
Transgenic Ungulates Having Reduced IgM Heavy Chain Protein
We discovered that bovines have two IgM heavy chain-encoding genes, each of which is expressed and undergoes VDJ rearrangement. Additionally, we have used homologous recombination to disrupt each of these genes in bovine fibroblasts, which can then be used in cloning methods to make transgenic bovines in which functional IgM is reduced, substantially eliminated, or eliminated.
Although only one IgM-encoding gene has been identified in other ungulates, it is likely, in view of our discovery, that additional such genes are present.
The transgenic ungulates of the invention are useful, for example, for the production of xenogeneic immunoglobulins and xenogeneic hematopoietic stem cells, and for the maintenance of xenogeneic tissues and organs in vivo.
Accordingly, the invention features a transgenic ungulate that is producing less than 10% of endogenous IgM heavy chain, relative to a control ungulate, and whose genome comprises a mutation of a gene encoding IgM heavy chain (e.g., bovine IgμU or bovine IgμAY). Desirably, the ungulate is producing less than 5%, 2%, or even 1%, relative to a matching control ungulate. Most desirably, the ungulate is producing no IgM heavy chain or IgM at levels below detection by western blot.
In one embodiment, the ungulate is a transgenic ungulate ungulate whose genome comprises a mutation in each of two genes encoding IgM heavy chain. Desirably, at least one mutation is a homozygous mutation, and more desirably both mutations are homozygous mutations. The mutation may be an insertion, deletion, or substitution, but most desirably is achieved by insertion of an exogenous nucleic acid, e.g., by homologous recombination. While the ungulate may still make some functional IgM heavy chain, it is most desirable that all functional IgM heavy chain is lost as a result of the mutations.
The invention is exemplified in a bovine, but is equally applicable to other ungulates (e.g., ovines, porcines, and caprines). In the case of bovines, the two IgM-encoding genes are IgμU and IgμAY.
The ungulate may be an adult ungulate, a fetal ungulate, or an ungulate embryo.
The invention also features (i) a transgenic ungulate somatic cell whose genome comprises a mutation of two genes encoding IgM heavy chain, as described above, and (ii) a transgenic bovine somatic cell whose genome comprises a hemizygous or homozygous mutation of IgμAY. Suitable cells include fibroblasts, epithelial cells, endothelial cells, neural cells, keratinocytes, hematopoietic cells, melanocytes, chondrocytes, lymphocytes (B-cells and T-cells), macrophages, monocytes, mononuclear cells, cardiac muscle cells, other muscle cells, and epidermal cells.
The invention further features methods for making xenogenous antibodies. One such method includes the steps of: (a) providing a transgenic ungulate of the invention, the ungulate having engrafted xenogenous hematopoietic stem cells; and (b) recovering xenogenous antibodies from this ungulate (e.g., from the serum or milk).
Another method for producing xenogenous antibodies includes the steps of:
The invention also features a method of expanding xenogenous hematopoietic stem cells by (a) providing a transgenic ungulate of the invention and having engrafted xenogenous hematopoietic stem cells; and (b) allowing the xenogenous hematopoietic stem cells to expand in said transgenic ungulate. If desired, the expanded hematopoietic stem cells can be collected from the transgenic ungulate.
The invention also features a method for maintaining a desired tissue or organ in vivo by: (a) providing a transgenic ungulate of the invention; (b) engrafting desired allogeneic or xenogeneic tissue or organ (e.g., skin, heart, lung, pancreatic, liver or kidney tissue) into the ungulate; and (c) maintaining the tissue or organ in the animal.
In any of the foregoing aspects of the invention, a transgenic ungulate may optionally have one or more nucleic acids encoding all or part of a xenogenous immunoglobulin gene that undergoes rearrangement and expresses one or more xenogenous immunoglobulins. In a preferred embodiment, the nucleic acid encoding all or part of a xenogenous gene is substantially human. Preferably, the nucleic acid encodes a xenogenous antibody, such as a human antibody or a polyclonal antibody. In various embodiments, the immunoglobulin chain or antibody is expressed in serum and/or milk. In other embodiments, the nucleic acid is contained within a chromosome fragment, such as a ΔHAC(FERM BP-7582, the International Patent Organism Depository, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 6, 1-1, Higashi 1-Chome Tsukuba-shi, Ibaraki-ken, 305-8566, Japan) or a ΔΔHAC (FERM BP-7581). In yet other embodiments, the nucleic acid is maintained in an ungulate cell independently from the host chromosome.
In still other embodiments of any of the aspects of the invention, the ungulate has a mutation of one or both alleles of an endogenous alpha-(1,3)-galactosyltransferase gene, PrP gene, and/or J chain gene. In other preferred embodiments, the ungulate has a nucleic acid encoding an exogenous J chain, such as a human J chain. Preferably, the mutation reduces or eliminates the expression of the endogenous alpha-(1,3)-galactosyltransferase enzyme, galactosyl(α1,3)galactose epitope, prion protein, and/or J chain. In still other preferred embodiments, the ungulate contains a xenogenous J chain nucleic acid, such as a human J chain nucleic acid. Preferably, the ungulate produces human IgA or IgM molecules containing human J chain. In various embodiments of the invention, the nucleic acid used to mutate an endogenous ungulate nucleic acid (e.g., a knockout cassette which includes a promoter operably linked to a nucleic acid encoding a selectable marker and operably linked to a nucleic acid having substantial sequence identity to the gene to be mutated) is not contained in a viral vector, such as an adenoviral vector or an adeno-associated viral vector. For example, the nucleic acid may be contained in a plasmid or artificial chromosome that is inserted into an ungulate cell, using a standard method such as transfection or lipofection that does not involve viral infection of the cell. In yet another embodiment, the nucleic acid used to mutate an endogenous ungulate nucleic acid (e.g., a knockout cassette which includes a promoter operably linked to a nucleic acid encoding a selectable marker and operably linked to a nucleic acid having substantial sequence identity to the gene to be mutated) is contained in a viral vector, such as an adenoviral vector or an adeno-associated viral vector. According to this embodiment, a virus containing the viral vector is used to infect an ungulate cell, resulting in the insertion of a portion or the entire viral vector into the ungulate cell.
Preferably, the ungulate is a bovine, ovine, porcine, or caprine. Preferably, the transgenic ungulate expresses an immunoglobulin chain or antibody from another genus, such as an antibody from any other mammal. Particularly preferred ungulates are sheep, big-horn sheep, goats, buffalos, antelopes, oxen, horses, donkeys, mule, deer, elk, caribou, water buffalo, camels, llama, alpaca, pigs, and elephants.
The invention also features a method of producing a transgenic ungulate (for example, a transgenic bovine) that rearranges and expresses a xenogenous (e.g., human) immunoglobulin gene locus. This may be accomplished, for example, by stably introducing a human chromosome fragment containing immunoglobulin genes into the ungulate, in order to produce a transgenic ungulate having B-cells that produce xenogenous immunoglobulins, in addition to or in lieu of endogenous immunoglobulins. This may also be accomplished by integrating a nucleic acid encoding a xenogenous immunoglobulin chain or xenogenous antibody into a chromosome of an ungulate. The transgenic ungulate has a mutation in at least two genes encoding IgM, such that the expression of endogenous IgM has been reduced or eliminated. In one embodiment, the invention features a method of producing a transgenic ungulate (for example, a transgenic bovine) in which at least two mu constant regions have been disrupted, and an artificial chromosome containing a gene locus encoding another species' immunoglobulin, preferably human, has been stably incorporated.
The invention also features a method of producing an ungulate somatic or embryonic stem (ES) cell, preferably a fibroblast or B-cell, wherein one or both alleles of at least two the endogenous IgM heavy chain genes have been disrupted, for example, by homologous recombination.
The invention also features a method of inserting, into an ungulate in which at least two the endogenous IgM heavy chain genes have been disrupted, a nucleic acid (for example, an artificial chromosome) that contains genes sufficient for the functional expression of non-ungulate immunoglobulins or their heavy or light chains. Preferably, these immunoglobulins are human immunoglobulins produced by introduction of nucleic acid encoding these immunoglobulins or immunoglobulin chains into an ungulate somatic cell, preferably a fibroblast, and producing cloned ungulates in which the nucleic acid is transmitted into the germ line.
The invention also features a method for introducing an artificial chromosome, preferably a human artificial chromosome (HAC), that contains genes that provide for immunoglobulin expression into the aforementioned homozygous knockout cells and generate ungulates that express non-ungulate immunoglobulins, preferably human immunoglobulins, in response to immunization and which undergo affinity maturation.
The invention also features methods for producing hybridomas and monoclonal antibodies using B-cells derived from the above-described transgenic ungulates (for example, transgenic bovines).
The invention also features methods for producing ungulate antiserum or milk that includes polyclonal human immunoglobulin by providing a transgenic ungulate described above that is producing polyclonal human immunoglobulins, and collecting ungulate antiserum or milk from the ungulate. Such human immunoglobulin, preferably human IgG, may be used as intravenenous immunoglobulin (IVIG) for the treatment or prevention of disease in humans. The polyclonal human immunoglobulins are preferably reactive against an antigen of interest.
Transgenic Ungulates Having Reduced Prion Protein
The present invention also features a bovine or bovine fetus that includes a non-naturally occurring mutation in one or both alleles of an endogenous prion nucleic acid. The mutation reduces, substantially eliminates, or eliminates the expression of functional prion protein, and may be hemizygous or homozygous. In addition, the bovine or bovine fetus may carry a further mutation that reduces the expression of an endogenous antibody. This mutation, for example, may reduce or substantially eliminate the expression of functional IgM heavy chain. Preferably, the bovine fetus is at least 30 days post-fertilization but may be any age up to birth. Preferred bovines include newborns and calves that are at least 1 week of age, and, more preferably, at least 2 months of age.
In a related aspect, the invention further features a product produced from a bovine or bovine fetus of the invention and a method of producing a product that substantially lacks prion protein, the method involving manufacturing the product in, or obtaining the product from, a bovine or bovine fetus of the invention. Exemplary products include milk, gelatin, collagen, and serum obtained from the bovine or bovine fetus, as well as a recombinant protein produced in the bovine or bovine fetus.
Methods for Producing Non-Human Mammals and Cells Containing Multiallelic or Multigenic Mutations
The invention provides a method of producing a cell having two genetic modifications (e.g., mutations or insertions of transgenes or artificial chromosomes). The method includes the steps of: (a) providing a non-human mammalian somatic cell having a first genetic modification; (b) inserting the cell or a progeny thereof, a chromatin mass from said cell or progeny thereof, or a nucleus from the cell or progeny thereof into a nucleated or enucleated oocyte; (c) transferring the oocyte obtained from step (b) or an embryo formed from the oocyte into a recipient; (d) isolating a cell from the embryo, or from a fetus or juvenile produced therefrom, wherein the cell contains the first genetic modification; and (e) introducing a second genetic modification into the genome of the cell of step (d) or a progeny thereof, thereby producing a cell having two genetic modifications.
The present invention thus provides methods for producing non-human mammals and cells containing multiallelic or multigenic mutations. Examples of such mammals are ungulates (e.g., bovines, ovines, porcines, caprines, equines, or buffaloes), rabbits, mice, rats, or primates (e.g., monkeys, baboons, or gorillas). Using the rejuvenation techniques provided herein, the lifespan of cells is increased, thereby allowing the genetic manipulation of cells on a long-term basis.
In one aspect, the present invention provides for producing a bovine cell containing multiallelic or multigenic mutations, involving the steps of: (a) providing a somatic ungulate cell having a mutation in a first allele of an endogenous gene; (b) inserting the cell or a progeny thereof, a chromatin mass from the cell or a progeny thereof, or a nucleus from the cell or a progeny thereof into a nucleated or enucleated oocyte; (c) transferring the oocyte obtained from step (b) or an embryo formed from this oocyte into the oviduct or uterus of a bovine; (d) allowing the transferred oocyte or the embryo of step (c) to develop into a fetus; (e) isolating a fetal cell from the bovine at between 25 and 90 days of gestation; and (f) introducing a mutation into the second allele of the first endogenous gene or into an allele of a different, second endogenous gene, thereby producing a bovine cell containing multiallelic or multigenic mutations. If desired, the method of the invention further involves, beginning with the cell obtained in step (f) or a progeny thereof, repeating the above method one or more times, thereby introducing mutations in additional alleles or genes.
The invention further provides a method for producing a non-human mammal (e.g., an ungulate) containing two genetic modifications, involving the steps of: (a) providing a non-human mammalian cell containing multiallelic or multigenic mutations (e.g., any one of the cells obtained above); (b) inserting the cell or a progeny thereof, a chromatin mass from the cell or a progeny thereof, or a nucleus from the cell or a progeny thereof into a nucleated or enucleated oocyte; (c) transferring the oocyte obtained from step (b) or an embryo formed from this oocyte into a non-human mammal; (d) allowing the transferred oocyte or the embryo of step (c) to develop into a non-human mammal, thereby producing a non-human mammal containing two genetic modifications. If desired, prior to step (b), the cell provided in step (a) may be permeabilized under conditions that allow chromatin condensation.
Exemplary non-human mammals of the invention are ungulates (e.g., bovines, ovines, porcines, caprines, equines, or buffalos), primates (e.g., monkeys, baboons, or gorillas), rabbits, mice, and rats. Desirably, a bovine (e.g., Bos taurus or Bos indicus) containing two genetic odifications is produced using the cloning methods described herein.
In all foregoing aspects of the invention, any somatic cell may be used (e.g., a cell from an embryo, fetus, calf, or adult). Exemplary cells include fibroblasts, epithelial cells, neural cells, epidermal cells, keratinocytes, hematopoietic cells, melanocytes, chondrocytes, B-lymphocytes, T-lymphocytes, erythrocytes, macrophages, monocytes, placental cells, and muscle cells. If the non-human mammal is a bovine, desirably, somatic cells are isolated from a fetus at between 25 to 90 days of gestation, between 35 to 60 days of gestation, between 35 and 50 days, between 35 and 45 days, between 38 and 43 days, and most preferably at about 40 days of gestation. Optionally, somatic cells may be permeabilized as described herein prior to the their insertion into oocytes.
One type of genetic modification is a mutation. Mutations may be introduced into transcriptionally active genes or transcriptionally silent genes. Exemplary endogenous genes in which mutations may be introduced are antibody-encoding genes (e.g., J chain or Igμgenes such as IgμU or IgμAY), genes encoding α-(1,3)-galactosyltransferase, or prion genes.
Typically, a mutation is introduced by the insertion of a polynucleotide (e.g., positive selection marker such as an antibiotic resistance gene) into an endogenous gene. Optionally, a mutation that is introduced in an endogenous gene reduces the expression of this gene. Alternatively, a mutation may also include the insertion of xenogenous nucleic acid molecule encoding for a polypeptide, such as an antibody. If desired, the polynucleotide may also contain recombinase sites flanking the positive selection marker, such as loxP sites, so that the positive selection marker may be removed by a recombinase (e.g., Cre recombinase).
Another type of genetic modification is a transgene that has been inserted into the cell's genome without introducing a mutation. One desirable transgene is an artificial chromosome such as a human artificial chromosome. The use of artificial chromosomes for producing xenogenous antibody (e.g., human antibody) is described herein.
As used herein, by “allele” is meant one member of a DNA pair that occupies a specific position on a specific chromosome.
By “artificial chromosome” is meant a mammalian chromosome or fragment thereof which has an artificial modification such as the addition of a selectable marker, the addition of a cloning site, the deletion of one or more nucleotides, the substitution of one or more nucleotides, and the like. By “human artificial chromosome (HAC)” is meant an artificial chromosome generated from one or more human chromosome(s). An artificial chromosome can be maintained in the host cell independently from the endogenous chromosomes of the host cell. In this case, the HAC can stably replicate and segregate along side endogenous chromosomes. Alternatively, it may be translocated to, or inserted into, an endogenous chromosome of the host cell. Two or more artificial chromosomes can be introduced to the host cell simultaneously or sequentially. For example, artificial chromosomes derived from human chromosome #14 (comprising the Ig heavy chain gene), human chromosome #2 (comprising the Ig kappa chain gene), and human chromosome #22 (comprising the Ig lambda chain gene) can be introduced. Alternatively, an artificial chromosome(s) comprising both a xenogenous Ig heavy chain gene and Ig light chain gene, such as ΔHAC, ΔΔHAC, or κHAC may be introduced. Preferably, the heavy chain loci and the light chain loci are on different chromosome arms (i.e., on different side of the centromere). In still other preferred embodiments, the total size of the HAC is less than or equal to approximately 12, 10, 9, 8, or 7 megabases.
By a “bovine fetus” is meant a bovine in utero that is at least 30 days post-fertilization.
By “cells derived from an embryo” is meant cells that result from the cell division of cells in the embryo.
By “chimeric embryo” is meant an embryo formed from cells from two or more embryos. The resulting fetus or offspring can have cells that are derived from only one of the initial embryos or cells derived from more than one of the initial embryos. If desired, the percentage of cells from each embryo that are incorporated into the placental tissue and into the fetal tissue can be determined using standard FISH analysis or analysis of a membrane dye added to one embryo.
By “chimeric ungulate” is meant an ungulate formed from cells from two or more embryos. The ungulate can have cells that are derived from only one of the initial embryos or cells derived from more than one of the initial embryos. If desired, the percentage of cells from each embryo that are incorporated into the placental tissue and into the fetal tissue can be determined using standard FISH analysis or analysis of a membrane dye added to one embryo.
By “chromatin mass” is meant more than one chromosome not enclosed by a membrane. Preferably, the chromatin mass contains all of the chromosomes of a cell. An artificially induced chromatin mass containing condensed chromosomes may be formed by exposure of a nucleus to a mitotic reprogramming media (e.g., a mitotic extract) as described herein. Alternatively, an artificially induced chromatin mass containing decondensed or partially condensed chromosomes may be generated by exposure of a nucleus to one of the following, as described herein: a mitotic extract containing an anti-NuMA antibody, a detergent and/or salt solution, or a protein kinase solution. A chromatin mass may contain discrete chromosomes that are not physically touching each other or may contain two or more chromosomes that are in physical contact.
If desired, the level of chromosome condensation may be determined using standard methods by measuring the intensity of staining with the DNA stain, DAPI. As chromosomes condense, this staining intensity increases. Thus, the staining intensity of the chromosomes may be compared to the staining intensity for decondensed chromosomes in interphase (designated 0% condensed) and maximally condensed chromosomes in mitosis (designated 100% condensed). Based on this comparison, the percent of maximal condensation may be determined. Preferred condensed chromatin masses are at least 20, 30, 40, 50, 60, 70, 80, 90, or 100% condensed. Preferred decondensed or partially condensed chromatin masses are less than 10% condensed.
By “days of gestation” is meant the days from the time that the oocyte or embryo is transferred into a uterus.
By “donor cell” is meant a cell from which a nucleus or chromatin mass is derived, or a permeabilized cell.
By “embryo” or “embryonic” is meant a developing cell mass that has not implanted into the uterine membrane of a maternal host. Hence, the term “embryo” may refer to a fertilized oocyte; an oocyte containing a donor chromatin mass, nucleus, or reprogrammed cell; a pre-blastocyst stage developing cell mass; or any other developing cell mass that is at a stage of development prior to implantation into the uterine membrane of a maternal host and prior to formation of a genital ridge. An embryo may represent multiple stages of cell development. For example, a one cell embryo can be referred to as a zygote; a solid spherical mass of cells resulting from a cleaved embryo can be referred to as a morula, and an embryo having a blastocoel can be referred to as a blastocyst. An “embryonic cell” is a cell isolated from or contained in an embryo.
By “embryo cloning” is meant the process in which an embryo is produced from a cell or cellular materials from another animal. Embryo cloning may be performed, for example, by inserting or fusing a donor cell, nucleus, or chromatin mass with an oocyte. The resulting oocyte or the embryo formed from this oocyte is then transferred into the uterus of an animal, thereby producing a cloned animal.
By “enrichment or depletion of a factor” is meant the addition or removal of a naturally-occurring or recombinant factor by at least 20, 40, 60, 80, or 100% of the amount of the factor originally present in a reprogramming media (e.g., a cell extract). Alternatively, a naturally-occurring or recombinant factor that is not naturally present in the reprogramming media may be added. Preferred factors include proteins such as DNA methyltransferases, histone deacetylases, histones, protamines, nuclear lamins, transcription factors, activators, and repressors; membrane vesicles, and organelles. In one preferred embodiment, the factor is purified prior to being added to the reprogramming media, as described below. Alternatively, one of the purification methods described below may be used to remove an undesired factor from the reprogramming media.
By “fetus” is meant a developing cell mass that has implanted into the uterine membrane of a maternal host. A “fetal cell” is any cell isolated from or contained in a fetus at any stage of gestation including birth.
By “fragment” is meant a polypeptide having a region of consecutive amino acids that is identical to the corresponding region of an antibody of the invention but is less than the full-length sequence. The fragment has the ability to bind the same antigen as the corresponding antibody based on standard assays, such as those described herein. Preferably, the binding of the fragment to the antigen is at least 20, 40, 60, 80, or 90% of that of the corresponding antibody.
By “gene” is meant a hereditary unit consisting of a sequence of DNA that occupies a specific location on a chromosome and determines a particular characteristic in an organism. A gene typically has two alleles.
By “hemizygous mutation” is meant that one allele of an endogenous gene has been mutated and the other allele has not been mutated.
By “homozygous mutation” is meant that two alleles of an endogenous gene have been mutated. According to this invention, the mutation-introducing event at both alleles may or may not be the same. Accordingly, two alleles of an endogenous gene genetically targeted by two different targeting vectors would be considered a homozygous mutation.
By “homozygous knock-out non-human mammal” is meant a mammal other than a human in which the two alleles of an endogenous gene have been genetically targeted, resulting in the marked reduction or elimination of expression of a functional gene product. According to this invention, the genetic targeting event at both alleles may or may not be the same. Accordingly, a non-human mammal, in which the two alleles of an endogenous gene have been genetically targeted by two different targeting vectors resulting in the null expression of the endogenous gene, would be considered as being a homozygous knock-out non-human mammal.
By “immortalized” is meant capable of undergoing at least 25, 50, 75, 90, or 95% more cell divisions than a naturally-occurring control cell of the same cell type, genus, and species as the immortalized cell or than the donor cell from which the immortalized cell was derived. Preferably, an immortalized cell is capable of undergoing at least 2-, 5-, 10-, or 20-fold more cell divisions than the control cell. More preferably, the immortalized cell is capable of undergoing an unlimited number of cell divisions. Immortalized cells include cells that naturally acquire a mutation in vivo or in vitro that alters their normal growth-regulating process. Still other preferred immortalized cells include cells that have been genetically modified to express an oncogene, such as ras, myc, abl, bcl2, or neu, or that have been infected with a transforming DNA or RNA virus, such as Epstein Barr virus or SV40 virus (Kumar et al. (1999) Immunol. Lett. 65:153-159; Knight et al. (1988) Proc. Natl. Acad. Sci. USA 85:3130-3134; Shammah et al. (1993) J. Immunol. Methods 160:19-25; Gustafsson and Hinkula (1994) Hum. Antibodies Hybridomas 5:98-104; Kataoka et al. (1997) Differentiation 62:201-211; Chatelut et al. (1998) Scand. J. Immunol. 48:659-666). Cells can also be genetically modified to express the telomerase gene (Roques et al. (2001) Cancer Res. 61:8405-8507).
By “knock-in mutation” is meant the insertion of an exogenous nucleic acid, optionally, encoding a polypeptide, into the chromosome of a cell.
By “mutation” is meant an alteration in a naturally-occurring or reference nucleic acid sequence, such as an insertion, deletion, frameshift mutation, silent mutation, nonsense mutation, or missense mutation. Preferably, the amino acid sequence encoded by the nucleic acid sequence has at least one amino acid alteration from a naturally-occurring sequence. Examples of recombinant DNA techniques for altering the genomic sequence of a cell, embryo, fetus, or mammal include inserting a DNA sequence from another organism (e.g., a human) into the genome, deleting one or more DNA sequences, and introducing one or more base mutations (e.g., site-directed or random mutations) into a target DNA sequence. Examples of methods for producing these modifications include retroviral insertion, artificial chromosome techniques, gene insertion, random insertion with tissue specific promoters, homologous recombination, gene targeting, transposable elements, and any other method for introducing foreign DNA. All of these techniques are well known to those skilled in the art of molecular biology. A “non-naturally occurring mutation” is one that is introduced artificially, for example, by recombinant means.
By “non-human mammal comprising multiallellic mutations” or “multiallelic non-human mammal” is meant a mammal other than a human in which two alleles of an endogenous gene have been mutated. The mutations in the two alleles may or may not be in the same location, and may or may not be due to the same type of alteration. For example, the alleles of the gene in a multiallelic non-human mammal may be mutated by the insertion of two different polynucleotide sequences.
By “non-human mammal comprising multigenic mutations” or “multigenic non-human mammal” is meant a mammal other than a human in which two or more different genes have been mutated. The mutations in the two genes may or may not be in the same location and may or may not be due to the same type of alteration. For example, two genes in a multigenic non-human mammal may be mutated by the insertion of two different polynucleotide sequences.
By “nucleus” is meant a membrane-bounded organelle containing most or all of the DNA of a cell. The DNA is packaged into chromosomes in a decondensed form. Preferably, the membrane encapsulating the DNA includes one or two lipid bilayers or has nucleoporins.
By “permeabilization” is meant the formation of pores in the plasma membrane or the partial or complete removal of the plasma membrane.
By “placenta” is meant the membranous vascular organ that develops in female mammals during pregnancy, lining the uterine wall and partially enveloping the fetus, to which it is attached by the umbilical cord.
By “purified” is meant separated from other components that naturally accompany it. Typically, a factor is substantially pure when it is at least 50%, by weight, free from proteins, antibodies, and naturally-occurring organic molecules with which it is naturally associated. Preferably, the factor is at least 75%, more preferably, at least 90%, and most preferably, at least 99%, by weight, pure. A substantially pure factor may be obtained by chemical synthesis, separation of the factor from natural sources, or production of the factor in a recombinant host cell that does not naturally produce the factor. Proteins, vesicles, and organelles may be purified by one skilled in the art using standard techniques such as those described by Ausubel et al. Current Protocols in Molecular Biology, John Wiley & Sons, 1995). The factor is preferably at least 2, 5, or 10 times as pure as the starting material, as measured using polyacrylamide gel electrophoresis, column chromatography, optical density, HPLC analysis, or western blot analysis (Ausubel et al., supra). Preferred methods of purification include immunoprecipitation, column chromatography such as immunoaffinity chromatography, magnetic bead immunoaffinity purification, and panning with a plate-bound antibody.
By “recloned” is meant used in a subsequent (e.g., second) round of cloning. In particular, a cell from an embryo, fetus, or adult generated from the methods of the invention may be incubated in a mitotic reprogramming media (e.g., a mitotic cell extract) to form a chromatin mass for insertion into an enucleated oocyte, as described above. Alternatively, the cell may be permeabilized, incubated in a reprogramming media, and inserted into an enucleated oocyte, as described above. Performing two or more rounds of cloning may result in additional reprogramming of the donor chromatin mass or donor cell, thereby increasing the chance of generating a viable offspring after the last round of cloning.
By “reducing the expression of an endogenous antibody” is meant reducing the amount of endogenous, functional antibodies produced by a B-cell or a population of B-cells. This reduction in the amount of endogenous antibodies may be due to a decrease in the amount of endogenous antibodies produced per B-cell, a decrease in the number of functional endogenous B-cells, or a combination thereof. Preferably, the amount of an endogenous antibody secreted by a B-cell or expressed on the surface of a B-cell expressing or secreting endogenous antibody is reduced by at least 25, 50, 75, 90, or 95%. In another preferred embodiment, the number of endogenous B-cells in a sample from the recipient mammal, such as a blood sample, is reduced by at least 25, 50, 75, 90, or 95%.
By “reprogramming media” is meant a solution that allows the removal of a factor from a cell, nucleus, chromatin mass, or chromosome or the addition of a factor from the solution to the cell, nucleus, chromatin mass, or chromosome. Preferably, the addition or removal of a factor increases or decreases the level of expression of an mRNA or protein in the donor cell, chromatin mass, or nucleus or in a cell containing the reprogrammed chromatin mass or nucleus. In another embodiment, incubating a permeabilized cell, chromatin mass, or nucleus in the reprogramming media alters a phenotype of the permeabilized cell or a cell containing the reprogrammed chromatin mass or nucleus relative to the phenotype of the donor cell. In yet another embodiment, incubating a permeabilized cell, chromatin mass, or nucleus in the reprogramming media causes the permeabilized cell or a cell containing the reprogrammed chromatin mass or nucleus to gain or lose an activity relative to the donor cell.
Exemplary reprogramming media include solutions, such as buffers, that do not contain biological molecules such as proteins or nucleic acids. Such solutions are useful for the removal of one or more factors from a nucleus, chromatin mass, or chromosome. Other preferred reprogramming medias are extracts, such as cellular extracts from cell nuclei, cell cytoplasm, or a combination thereof. Exemplary cell extracts include extracts from oocytes (e.g., mammalian, vertebrate, or invertebrate oocytes), male germ cells (mammalian, vertebrate, or invertebrate germ cells such as spermatogonia, spermatocyte, spermatid, or sperm), and stem cells (e.g., adult or embryonic stem cells). Yet other reprogramming media are solutions or extracts to which one or more naturally-occurring or recombinant factors (e.g., nucleic acids or proteins such as DNA methyltransferases, histone deacetylases, histones, protamines, nuclear lamins, transcription factors, activators, repressors, chromatin remodeling proteins, growth factors, interleukins, cytokines, or other hormones) have been added, or extracts from which one or more factors have been removed. Still other reprogramming media include solutions of detergent (e.g., 0.01% to 0.1%, 0.1% to 0.5%, or 0.5% to 2% ionic or non-ionic detergent such as one or more of the following detergents: SDS, Triton X-100, Triton X-114, CHAPS, Na-deoxycholate, n-octyl glucoside, Nonidet P40, IGEPAL, Tween 20, Tween 40, or Tween 80), salt (e.g., ˜0.1, 0.15, 0.25, 0.5, 0.75, 1, 1.5, or 2 M NaCl or KCl), polyamine (e.g., ˜1 μM, 10 μM, 100 μM, 1 mM or 10 mM spermine, spermidine, protamine, or poly-L-lysine), a protein kinase (e.g., cyclin-dependent kinase 1, protein kinase C, protein kinase A, MAP kinase, calcium/calmodulin-dependent kinase, CK1 casein kinase, or CK2 casein kinase), and/or a phosphatase inhibitor (e.g., ˜10 μM, 100 μM, 1 mM, 10 mM, 50 mM, 100 mM of one or more of the following inhibitors: Na-orthovanadate, Na-pyrophosphate, Na-fluoride, NIPP1, inhibitor 2, PNUTS, SDS22, AKAP149, or ocadaic acid) or nuclioplasmin. In some embodiments, the reprogramming medium contains an anti-NuMA antibody. If desired, multiple reprogramming media may be used simultaneously or sequentially to reprogram a donor cell, nucleus, or chromatin mass.
By “reprogrammed cell” is meant a cell that has been exposed to a reprogramming media. Preferably, at least 1, 5, 10, 15, 20, 25, 50, 75, 100, 150, 200, 300, or more mRNA or protein molecules are expressed in the reprogrammed cell that are not expressed in the donor or permeabilized cell. In another preferred embodiment, the number of mRNA or protein molecules that are expressed in the reprogrammed cell, but not expressed in the donor or permeabilized cell, is between 1 and 5, 5 and 10, 10 and 25, 25 and 50, 50 and 75, 75 and 100, 100 and 150, 150 and 200, or 200 and 300, inclusive. Preferably, at least 1, 5, 10, 15, 20, 25, 50, 75, 100, 150, 200, 300, or more mRNA or protein molecules are expressed in the donor or permeabilized cell that are not expressed in the reprogrammed cell. In yet another preferred embodiment, the number of mRNA or protein molecules that are expressed in the donor or permeabilized cell, but not expressed in the reprogrammed cell, is between 1 and 5, 5 and 10, 10 and 25, 25 and 50, 50 and 75, 75 and 100, 100 and 150, 150 and 200, or 200 and 300, inclusive. In still another preferred embodiment, these mRNA or protein molecules are expressed in both the donor cell (i.e., the donor or permeabilized starting cell) and the reprogrammed cell, but the expression levels in these cells differ by at least 2, 5, 10, or 20-fold, as measured using standard assays (see, for example, Ausubel et al., supra).
By “substantially identical” is meant having a sequence that is at least 80, 90, 95, 98, or 100% identical to that of another sequence. Sequence identity is typically measured using BLAST® (Basic Local Alignment Search Tool) or BLASTS 2 with the default parameters specified therein (see, Altschul et al. (1990) J. Mol. Biol. 215:403-410; and Tatiana et al. (1999) FEMS Microbiol. Lett. 174:247-250). These software programs match similar sequences by assigning degrees of homology to various substitutions, deletions, and other modifications. Conservative substitutions typically include substitutions within the following groups: glycine, alanine, valine, isoleucine, leucine; aspartic acid, glutamic acid, asparagine, glutamine; serine, threonine; lysine, arginine; and phenylalanine, tyrosine.
By “viable offspring” is meant an animal that survives ex utero. Preferably, the mammal is alive for at least one second, one minute, one hour, one day, one week, one month, six months, or one year from the time it exits the maternal host. The animal does not require the circulatory system of an in utero environment for survival.
Other features and advantages of the invention will be apparent from the following detailed description and from the claims.
In general, the invention features transgenic non-human mammals (e.g., bovines and other ungulates), and methods of making these mammals. In particular, the invention features transgenic ungulates having reduced levels of endogenous IgM heavy chain and/or prion protein. The invention is described in more detail below.
Methods for Producing Non-Human Mammals and Cells Containing Multiallelic or Multigenic Mutations
The present invention provides rapid methods to sequentially target transcriptionally silent and transcriptionally active genes in non-human mammals, such as cattle (e.g., bovine). More specifically, we have discovered a sequential gene targeting system that we first used to knock out both alleles of a silent gene, the bovine immunoglobulin μU (IgμU) gene, producing heterozygous and homozygous knockout calves. IgμU targeting was followed by sequential knockout targeting of both alleles of a transcriptionally active gene in fibroblasts, the bovine prion protein (PrP) gene, in the IgμU−/− cell line to produce double homozygous knockout fetuses. Using the methods of the invention, gene targeting can be performed sequentially in somatic cells, resulting in the production of multiallelic or multigenic non-human mammals. The present invention further employs cloning methods to rejuvenate cell lines following each round of targeting, as described in U.S. Patent Application Publication No. 2003-0046722, hereby incorporated by reference.
The disruption of a gene of interest first involves the production of hemizygous gene knockout cells and the production of a fetus by embryonic cloning. Genetically targeted cells are next harvested from the resulting fetuses at any time during gestation. In a bovine, such cell harvesting desirably occurs at between 25 to 90 days of gestation, at between 35 to 60 days of gestation, at between 35 to 50 days of gestation, preferably at between 35 to 45 days, more preferably at between 38 to 43 days, and most preferably at about 40 days of gestation. Next, the second allele of the same gene locus, or alternatively, an allele of a different endogenous gene is targeted in the harvested cells. These cells are next used to derive fetuses, from which somatic cells such as fibroblasts may further be isolated and used for further rounds of cloning. The above steps may then be repeated until cells containing the desired multiallelic or multigenic mutations are generated. If desired, these cells may be used to produce non-human mammals, such as ungulates.
Using the methods of the present invention, we show that each targeting event required approximately 2.5 months from transfection to establishment of regenerated cell lines, such that a single homozygous genetic modification could be made in calves in 14 months (including five months for targeting two alleles and nine months gestation) and a double homozygous modification could be made in 19 months. In contrast, breeding a heterozygous founder to produce homozygous calves would require approximately five years, making the generation of double homozygous calves from two heterozygous founders impractical. Using the sequential targeting strategy described herein, complex genetic modifications in large animal species are not only feasible but also straightforward and useful for many applications.
Genetically modified large animals are, in many cases, more suitable models for the study of human disease than mice, such as for the study of human cystic fibrosis, for example (Harris (1997) Hum. Mol. Genet. 6:2191-2194). Homozygous knockout targeting may also be useful, for example, for the elimination of bovine antibodies when attempting to produce human polyclonal antibodies in a genetically modified cow (Kuroiwa (2002) Nature Biotechnol. 20:889-894). Furthermore, the inactivation of the IgμU gene, for example, may be useful in investigating bovine immunology, such as the mechanism of B-cell development, which is known to be substantially different from that of humans or mice (Butler (1997) Rev. Sci. Tech. 17:43-70). Pigs carrying multiple KOs, not only for α-1,3-galactosyltransferase gene (Phelps et al. (2003) Science 299:411-414), but also for other genes, such as major histocompatibility complex (MHC) genes, may also be a more suitable source of organs and tissue for xenotransplantion. In agriculture, homozygous knockout targeting could be useful in improving the safety and disease-resistance of animals, such as the inactivation of the bovine prion gene, and elimination of the “mad-cow disease” threat. The methods of the invention are therefore useful for complex genetic modifications in animals for gene-functional analysis, as well as biomedical and agricultural applications, without the necessity of germ line transmission.
Transgenic Ungulates Having Reduced IgM Heavy Chain Protein
We have determined that there are two genes encoding bovine IgM heavy chains. The first published sequence asserted to encode bovine IgM is shown in
We now demonstrate that bovines contain and express two Igμ genes, which we refer to as IgμU and IgμAY. As both genes are expressed, to produce a bovine having no IgM heavy chain protein requires mutation of both genes.
Although only one IgM-encoding gene has been identified in other ungulates, it is likely, in view of our discovery of two Igμ genes in bovines, that additional such genes are present. These additional genes can be identified using standard techniques, such as those described herein.
To alter immunoglobulin genes of other ungulates, targeting vectors are designed, to contain three main regions. The first region is homologous to the locus to be targeted. The second region is a drug selection marker that specifically replaces a portion of the targeted locus. The third region, like the first region, is homologous to the targeted locus but is not contiguous with the first region in the wild type genome. Homologous recombination between the targeting vector and the desired wild type locus results in deletion of locus sequences between the two regions of homology represented in the targeting vector and replacement of that sequence with a drug resistance marker. In preferred embodiments the total size of the two regions of homology is approximately 6 kilobases, and the size of the second region that replaces a portion of the targeted locus is approximately 2 kilobases. This targeting strategy is broadly useful for a wide range of species from prokaryotic cells to human cells. The uniqueness of each vector used is in the locus chosen for gene targeting procedures and the sequences employed in that strategy. This approach may be used in all ungulates, including, without limitation, goats (Capra hircus), sheep (Ovis aries), and the pig (Sus scrofa), as well as cattle (Bos taurus and Bos indicus).
The use of electroporation for targeting specific genes in the cells of ungulates may also be broadly used in ungulates. The general procedure described herein is adaptable to the introduction of targeted mutations into the genomes of other ungulates. Modification of electroporation conditions (voltage and capacitance) may be employed to optimize the number of transfectants obtained from other ungulates.
In addition, the strategy used herein to target the heavy chain locus in cattle (i.e., removal of all coding exons and intervening sequences using a vector containing regions homologous to the regions immediately flanking the removed exons) may also be used equally well in other ungulates. For example, extensive sequence analysis has been performed on one immunoglobulin heavy chain locus of sheep (Ovis aries), and the sheep locus is highly similar to the bovine locus in both structure and sequence (GenBank accession nos. Z71572, Z49180 through Z49188, M60441, M60440, AF172659 through AF172703). In addition to the large number of cDNA sequences reported for rearranged Ovis aries immunoglobulin chains, genomic sequence information has been reported for the heavy chain locus, including the heavy chain 5′ enhancer (GenBank accession no. Z98207), the 3′ mu switch region (GenBank accession no. Z98680) and the 5′ mu switch region (GenBank accession no. Z98681). The complete mRNA sequence for the sheep secreted-form of the heavy chain has been deposited as GenBank accession no. X59994. This deposit contains the entire sequence of four coding exons, which are very similar to the corresponding bovine sequence.
Thus, the present invention relates to the production of a transgenic ungulate, preferably a transgenic cow, in which endogenous IgM expression has been reduced or eliminated by the mutation of two or more Ig, genes. Optionally, a nucleic acid (e.g., an artificial chromosome) has been stably introduced that comprises genes that are sufficient for the production of functional antibodies of another species, preferably human.
Transgenic Ungulates Having Reduced Prion Protein
As described herein, Applicants have successfully produced a healthy PrP-deficient calf by means of sequential gene targeting. Such animals provide an improved population for production of BSE-free bovine-derived agricultural and biomedical materials, including milk, gelatin, collagen, and serum, as well as an improved source for human recombinant protein production for human therapy and medical materials for human xenotransplantation. The bovine or bovine fetuses of the invention may be used to produce any product normally derived agriculturally from such animals. They may also be used to produce any recombinant protein, including, without limitation, human antibodies, for example, by any of the methods described in U.S. Patent Application Publication Nos. 2003-0037347, 2004-0068760, and 2003-0056237, or PCT Publication No. WO2004/044156, all hereby incorporated by reference.
Non-Human Mammalian Cells
As discussed herein, the methods of the present invention involve the introduction of mutations into somatic non-human mammalian cells, such as somatic ungulate cells. Suitable somatic cells include cells from embryos, fetuses, calves, or adult animals. Preferred cells for gene targeting include differentiated cells such as fibroblasts, epithelial cells, neural cells, epidermal cells, keratinocytes, hematopoietic cells, melanocytes, chondrocytes, B-lymphocytes, T-lymphocytes, erythrocytes, macrophages, monocytes, placental, and muscle cells. Preferred cells also include those from any organ, such as the bladder, brain, esophagus, fallopian tube, heart, intestines, gallbladder, kidney, liver, lung, ovaries, pancreas, prostate, spinal cord, spleen; stomach, testes, thymus, thyroid, trachea, ureter, urethra, and uterus. Preferably, the donor cell, donor nucleus, donor chromatin mass, or reconstituted oocyte is not tetraploid.
Cells may be derived from any non-human mammal, including an ungulate, rabbit, mouse, rat, or primate. Ungulates include members of the orders Perissodactyla and Artiodactyla, such as any member of the genus Bos. Other preferred ungulates include sheep, big-horn sheep, goats, buffalos, antelopes, oxen, horses, donkeys, mule, deer, elk, caribou, water buffalo, camels, llama, alpaca, pigs, and elephants. Most preferably, the non-human mammal is a bovine (e.g., Bos taurus or Bos indicus).
If a cell to be genetically targeted is derived from an embryo or a fetus, the cell may be isolated at any time during the gestation period until the birth of the genetically altered non-human mammal. As discussed above, bovine cells are desirably isolated at between 25 to 90 days of gestation, between 35 to 60 days of gestation, between 35 to 50 days, preferably between 35 to 45 days, more preferably between 38 to 43 days, and most preferably at about 40 days of gestation. Ovine cells are desirably isolated at between 25 to 150 days of gestation, between 30 to 100 days, preferably between 35 to 80 days, more preferably between 35 to 60 days, and most preferably at about 40 days of gestation. Equine cells are desirably isolated at between 25 to 300 days of gestation, between 30 to 100 days, preferably between 35 to 80 days, more preferably between 35 to 60 days, and most preferably at about 40 days of gestation. Porcine cells are desirably isolated at between 25 to 110 days of gestation, between 30 to 90 days, preferably between 30 to 70 days, more preferably between 30 to 50 days, and most preferably at about 35 days of gestation. Caprine cells are desirably isolated at between 25 to 150 days of gestation, between 30 to 100 days, preferably between 35 to 80 days, more preferably between 35 to 60 days, and most preferably at about 40 days of gestation. Primate cells are desirably isolated at between 25 to 150 days of estation, between 30 to 100 days, preferably between 35 to 80 days, more preferably between 35 to 60 days, and most preferably at about 40 days of gestation. Rodent cells are desirably isolated at between 6 to 18 days of gestation, between 8 to 16 days, preferably between 10 to 16 days, more preferably between 12 to 16 days, and most preferably at about 14 days of gestation.
The recipient cell is preferably an oocyte, a fertilized zygote, or a two-cell embryo, all of which may or may not have been enucleated. Typically, the donor and the recipient cell are derived from the same species. However, there has been success reported in achieving development from embryos reconstructed using donor and recipient cells from different species.
Gene Targeting
In general, the genetic targeting events of the invention may include inactivation, removal, or modification of a gene; upregulation of a gene; gene replacement; or transgene replacement at a predetermined locus. Examples of genes that may be targeted resulting in their inactivation, removal, or modification are genes encoding antigens which are xenoreactive to humans (e.g., α-1,3 galactosyltransferase); antibody-encoding genes; genes in the PrP locus responsible for the production of the prion protein and its normal counterpart in non-human animals; genes which in humans are responsible for genetic disease and which in modified, inactivated, or deleted form could provide a model of that disease in animals (e.g., the cystic fibrosis transmembrane conductance regulator gene); genes responsible for substances which provoke food intolerance or allergy; genes responsible for the presence of particular carbohydrate residues on glycoproteins (e.g., the cytidine monophospho-N-acetyl neuraminic acid hydroxylase gene in non-human animals); and genes responsible for the somatic rearrangement of immunoglobulin genes, such as RAG1 and RAG2.
Among genes that can be targeted resulting in their upregulation are genes responsible for suppression of complement-mediated lysis (e.g., porcine CD59, DAF, and MCP). Furthermore and as described further below, replacement of genes may also be performed. Genes that may be replaced include genes responsible for the production of blood constituents (e.g., serum albumin), genes responsible for substances that provoke food intolerance or allergy, immunoglobulin genes, and genes responsible for surface antigens.
Using the methods of the present invention, the time necessary for the identification, isolation, analysis, and expansion of primary cell clones carrying targeted events is significantly minimized. This is an important aspect of the invention because reduction of the time in culture increases the likelihood that cells used as nuclear donors are viable, normal, and euploid.
Targeting Constructs
Targeted gene mutation requires generating a nucleic acid construct having regions of homology to the targeted allele in the gene of interest such that integration of the construct into the genomic allele disrupts its expression. Thus, to alter a gene, a targeting vector is designed to contain three main regions. The first region is homologous to the locus to be targeted. The second region is a polynucleotide sequence (e.g., encoding a selection marker such as an antibiotic resistance protein) that specifically replaces a portion of the targeted locus. The third region, like the first region, is homologous to the targeted locus but is not contiguous with the first region in the wild type genome. Homologous recombination between the targeting vector and the desired wild-type locus results in deletion of locus sequences between the two regions of homology represented in the targeting vector and replacement of that sequence, for example, with a drug resistance marker. The uniqueness of each vector used is in the locus chosen for gene targeting procedures and the sequences employed in that strategy. This approach may be used in all mammals, including ungulates such as, goats (Capra hircus), sheep (Ovis aries), pigs (Sus scrofa), and cattle (Bos taurus or Bos indicus). Exemplary vectors for carrying out such targeted mutation are described herein. Methods for constructing vectors that provide for homologous recombination at other targeted sites are well known to those skilled in the art. Moreover, the construction of a suitable vector is within the level of skill in the art.
In order to facilitate homologous recombination, the vectors used to effect homologous recombination and inactivation of a gene of interest, respectively, contain portions of DNA that exhibit substantial sequence identity to the genes to be targeted. Preferably, these sequences have at least 98% sequence identity, more preferably, at least 99% sequence identity, and even 100% sequence identity with the targeted gene loci to facilitate homologous recombination. In preferred embodiments, the total size of the two regions of homology is approximately 9-9.5 kilobases and the size of the second region that replaces a portion of the targeted locus is approximately 2 kilobases.
Typically, the construct includes a marker gene that allows for the selection of desired homologous recombinants, for example, cells in which the gene of interest has been disrupted by homologous recombination. Marker genes include antibiotic resistance markers, drug resistance markers, and green fluorescent protein, among others. One neomycin resistance construct was assembled as follows. A construct designated “pSTneoB” (Katoh et al. (1987) Cell Struct. Funct. 12:575; Japanese Collection of Research Biologicals (JCRB) deposit number: VE039) was designed to contain a neomycin resistance gene under the control of an SV40 promoter and TK enhancer upstream of the coding region. Downstream of the coding region is an SV40 terminator sequence. The neo cassette was excised from “pSTneoB” as an XhoI fragment. After the ends of the fragment were converted to blunt ends using standard molecular biology techniques, the blunt ended fragment was cloned into the EcoRV site in the vector, pBS246 (Gibco/Life Technologies). This site is flanked by loxP sites. The new construct, designated “pLoxP-STNeoR”, was used to generate the mu knockout DNA construct. The desired fragment of this construct is flanked by loxP sites and NotI sites, which were originally present in the pBS246 cloning vector. The desired NotI fragment, which contains loxP-neo-loxP, was used for replacement of the immunoglobulin mu constant region exons. The SV40 promoter operably linked to the neomycin resistance gene activates the transcription of the neomycin resistance gene, allowing cells in which the desired NotI fragment has replaced the mu constant region exons to be selected based on their resulting antibiotic resistance.
The strategy used herein to target genes in cattle (i.e., removal of a portion of the coding region and intervening sequences using a vector containing regions homologous to the regions immediately flanking the removed exons) may also be used in other mammals. For example, extensive sequence analysis has been performed on one immunoglobulin heavy chain locus of sheep (Ovis aries), and the sheep locus is highly similar to the bovine locus in both structure and sequence (GenBank accession nos. Z71572, Z49180 through Z49188, M60441, M60440, AF172659 through AF172703). In addition to the large number of cDNA sequences reported for rearranged Ovis aries immunoglobulin chains, genomic sequence information has been reported for the heavy chain locus, including the heavy chain 5′ enhancer (GenBank accession no. Z98207), the 3′ mu switch region (GenBank accession no. Z98680) and the 5′ mu switch region (GenBank accession no. Z98681). The complete mRNA sequence for the sheep secreted form of the heavy chain has been deposited as GenBank accession no. X59994. This deposit contains the entire sequence of four coding exons, which are very similar to the corresponding bovine sequence. Accordingly, the GenBank sequence may be used to determine areas of high homology with the bovine IgpU sequence for the design of PCR primers. Because non-isogenic DNA was used to target bovine cells, finding areas of high homology with sheep sequence was used as an indicator that similar conservation of sequences between breeds of cow was likely. Given the similarity between the sequences and structures of the bovine and ovine immunoglobulin loci, the targeting strategies used herein to remove bovine immunoglobulin loci may be applied to the ovine system. In addition, existing information on pigs (Sus scrofa; GenBank accession no. S42881) and goats (Capra hircus; GenBank accession no. AF140603) indicates that the immunoglobulin loci of both of these species are also sufficiently similar to the bovine loci to utilize the present targeting strategies.
In one method of targeting cells, the targeting construct includes regulatory expression for driving expression of the marker gene, as well as a polyadenylation signal sequence. Such a construct allows for detection of the inserted sequence independent of the expression of the mutagenized gene and thus permits the identification of recombinants in silent genes (i.e., genes that are not expressed in fibroblasts). In order to determine whether the marker gene integrated into the genome by means of homologous recombination rather than through random insertion, one may use standard molecular biology techniques such as Southern blotting, PCR, or DNA sequencing.
Selection of Targeted Cells
Genetically targeted cells are typically identified using a selectable marker. If a cell already contains a selectable marker however, a new targeting construct containing a different selectable marker may be required. Alternatively, if the same selectable marker is employed, cells may be selected in the second targeting round by raising the drug concentration (for example, by doubling the drug concentration).
Targeting constructs may also contain selectable markers flanked by loxP sites to facilitate the efficient deletion of the marker using the Cre/lox system. Thus, at some point after the gene targeting event and preferably before any embryo cloning, such excision may be performed to remove portions of genetic material from the cell. This material may be a selectable marker or an introduced genetic transcription activator. This removal may be carried out by procedures described hereinafter, or by other procedures well known in the art.
In one example, fetal fibroblasts carrying the targeting vector are transfected via electroporation with a Cre containing plasmid (e.g., a Cre plasmid that contains a GFPCre fusion gene as described by Gagneten et al. (1997) Nucleic Acids Res. 25:3326-3331). This allows for the rapid selection of all clones that contain a Cre protein. In this regard, cells are selected either by FACS sorting or by manual harvesting of green fluorescing cells via micromanipulation. Cells that are green are expected to carry actively transcribed Cre recombinase, which would remove the drug resistance marker. Cells selected for Cre expression are cloned and analyzed for the deletion of the drug resistance marker by PCR analysis. Following such confirmation, such cells are used for the next round of genetic targeting or for cloning.
Introduction of Xenogenous Nucleic Acids
If desired, xenogenous nucleic acid molecules encoding a desired polypetide may be inserted into an endogenous gene as part of the introduced mutation. For example, genes encoding antibodies of a particular species may be introduced into an endogenous gene. Preferably, human artificial chromosomes are used for this purpose, such as those disclosed in PCT Publication Nos. WO97/07671 and WO00/10383, each hereby incorporated by reference. These human artificial chromosomes also are described in a corresponding issued Japanese Patent JP 30300092. The construction of artificial human chromosomes that contain and express human immunoglobulin genes is disclosed in Shen et al. (1997) Hum. Mol. Genet. 6:1375-1382; Kuroiwa et al. (2000) Nature Biotechnol. 18:1086-1090; and Loupert et al. (1998) Chromosome 107:255-259. Following the stable insertion of the artificial chromosome, the cell line (e.g., a bovine fetal fibroblast) may be used as a donor cell for further gene targeting.
As an alternative to the use of human artificial chromosome, polynucleotides encoding genes of interest may also be integrated into the chromosome using a YAC vector, BAC vector, or cosmid vector. Such vectors may be introduced into cells (e.g., fetal fibroblasts cells) using known methods, such as electroporation, lipofection, fusion with a yeast spheroplast comprising a YAC vector, and the like. Desirably, vectors containing genes of interest may be targeted to the endogenous corresponding gene loci of the cells (e.g., fetal fibroblasts), resulting in the simultaneous introduction of the gene of interest and the mutation of the endogenous gene.
Integration of a nucleic acid encoding a gene of interest may also be carried out as described in the patents by Lonberg et al. (U.S. Pat. Nos. 5,545,806, 5,569,825, 5,625,126, 5,633,425, 5,661,016, 5,750,172, 5,770,429, 5,789,650, 5,814,318 5,874,299, 5,877,397, and 6,300,129, all of which are hereby incorporated by reference). In the “knock-in” construct used for the insertion of gene of interest into a chromosome of a host mammal, one or more genes and an antibiotic resistance gene may be operably-linked to a promoter which is active in the cell type transfected with the construct. For example, a constitutively active, inducible, or tissue-specific promoter may be used to activate transcription of the integrated antibiotic resistance gene, allowing transfected cells to be selected based on their resulting antibiotic resistance. Alternatively, a knock-in construct in which the knock-in cassette containing the gene(s) of interest and the antibiotic resistance gene is not operably linked to a promoter may be used. In this case, cells in which the knock-in cassette integrates downstream of an endogenous promoter may be selected based on the resulting expression of the antibiotic resistance marker under the control of the endogenous promoter. These selected cells may be used in the embryo cloning procedures described herein to generate a transgenic non-human mammal containing a gene of interest integrated into a host chromosome. Alternatively, an animal containing exogenous genes of interest may be mated with an animal in which the endogenous gene is inactivated.
Exemplary Gene Mutations
Any number of exemplary gene mutations may be introduced into mammals using the present methods. For example, the endogenous ungulate Ig J chain gene may be knocked out to prevent the potential antigenicity of the ungulate Ig J chain in the antibodies of the invention that are administered to humans. For the construction of the targeting vector, the cDNA sequence of the bovine Ig J chain region found in GenBank accession number U02301 may be used. This cDNA sequence may be used as a probe to isolate the genomic sequence of bovine Ig J chain from a BAC library such as RPC1-42 (BACPAC in Oakland, Calif.) or to isolate the genomic sequence of the J chain from any other ungulate. Additionally, the human J chain coding sequence may be introduced into the ungulates of the present invention for the functional expression of human IgA and IgM molecules. The cDNA sequence of human J chain is available as GenBank accession numbers AH002836, M12759, and M12378. This sequence may be inserted into an ungulate fetal fibroblast using standard methods, such as those described herein. For example, the human J chain nucleic acid in a HAC, YAC vector, BAC vector, cosmid vector, or knock-in construct may be integrated into an endogenous ungulate chromosome or maintained independently of endogenous ungulate chromosomes. The resulting transgenic ungulate cells may be used in the embryo cloning methods described herein to generate the desired ungulates that have a mutation that reduces or eliminates the expression of functional ungulate J chain and that contain a xenogenous nucleic acid that expresses human J chain.
In another example, if a non-human mammal, such as an ungulate, is genetically engineered to produce a human antibody, it may be desirable to also reduce or eliminate the expression of the ungulate α-(1,3)-galactosyltransferase gene, a gene that encodes an enzyme that produces the galactosyl(α1,3)galactose epitope. Glycosylated human antibodies modified by this carbohydrate epitope are sometimes inactivated or eliminated when administered as therapeutics to humans by recipient antibodies reactive with the epitope. To eliminate this possible immune response, the sequence of bovine α-(1,3)-galactosyltransferase gene may be used to design a knockout construct to inactive this gene. The bovine sequence (GenBank accession number J04989; Joziasse et al. (1989) J. Biol. Chem. 264: 14290-14297) or the porcine α-(1,3)-galactosyltransferase sequence (disclosed in U.S. Pat. Nos. 5,821,117 and 6,153,428) may be used to inactivate the genes in those species or to obtain the genomic α-(1,3)-galactosyltransferase sequence from a variety of other ungulates to generate mammals with reduced or eliminated expression of the epitope.
The ungulate PrP gene (encoding prion protein) may also be mutated or inactivated to reduce the potential risk of an infection such as bovine spongiform encephalopathy (BSE). Mutation of the bovine PrP gene is described below.
The additional mutations or the gene inactivation mentioned above may be incorporated into the ungulates of the present invention using various methodologies. Once a transgenic ungulate cell line is generated for each desired mutation, crossbreeding may be used to incorporate these additional mutations into the ungulates of the present invention. Alternatively, fetal fibroblast cells which have these additional mutations can be used as the starting material for the knockout of endogenous immunoglobulin genes and/or the introduction of xenogenous immunoglobulin genes. Also, as is described herein, fetal fibroblast cells having a knockout mutation in endogenous immunoglobulin genes and/or containing xenogenous immunoglobulin genes can be uses as a starting material for these additional mutations or inactivations.
Production of Cloned Non-Human Mammals
We have previously disclosed a variety of methods for cloning mammals (e.g., ungulates, such as bovines) that may be used to clone mammals with one or more mutations in genes encoding IgM heavy chain (see, e.g., U.S. Patent Application Publication No. 2002-0046722 and PCT Publication No. WO02/051997). In some of these methods, a permeabilized cell is incubated with a reprogramming media (e.g., a cell extract) to allow the addition or removal of factors from the cell, and then the plasma membrane of the permeabilized cell is resealed to enclose the desired factors and restore the membrane integrity of the cell. Some of these methods also involve the condensation of a donor nucleus (e.g., an isolated nucleus or a nucleus within a donor cell) into a chromatin mass to allow the release of nuclear components such as transcription factors that may promote the transcription of genes that are undesirable for the development of the nuclear transplant embryo into a viable offspring. If desired, the steps of any of these methods may be repeated one or more times or different reprogramming methods may be performed sequentially to increase the extent of reprogramming, resulting in greater viability of the cloned fetuses.
Other methods for the production of cloned mammals (e.g., bovines) and cloned transgenic non-human mammals are known in the art, described, for example, in U.S. Pat. No. 5,995,577, assigned to University of Massachusetts, and in PCT Publication Nos. WO95/16670; WO96/07732; WO97/0669; and WO97/0668 (collectively, “the Roslin methods”). The Roslin methods differ from the University of Massachusetts techniques in that they use quiescent rather than proliferating donor cells. All of these patents are incorporated by reference herein in their entirety. These techniques are not limited to use for the production of transgenic bovines; the above techniques may be used for embryo cloning of other non-human mammals such as ungulates as well.
Following embryo cloning, production of desired animals may be affected either by mating the ungulates or by secondary gene targeting using the homologous targeting vector previously described.
Cre/Lox Excision of the Drug Resistance Marker
In one embodiment of the invention, the Cre/lox system is used to facilitate the efficient deletion of the marker following. Fetal fibroblasts carrying the targeting vector are transfected via electroporation with a Cre containing plasmid. A Cre plasmid that contains a GFPcre fusion gene (Gagneten et al. (1997) Nucleic Acids Res. 25:3326-3331) may be used. This allows the rapid selection of all clones that contain Cre protein. These cells are selected either by FACS sorting or by manual harvesting of green fluorescing cells via micromanipulation. Cells that are green are expected to carry actively transcribed Cre recombinase and hence delete the drug resistance marker. Cells selected for Cre expression are cloned and clones analyzed for the deletion of the drug resistance marker via PCR analysis. Those cells that are determined to have undergone excision are grown to small clones, split and one aliquot is tested in selective medium to ascertain with certainty that the drug resistance gene has been deleted. The other aliquot is used for the next round of targeted deletion.
Methods for Breeding Ungulates
In preferred embodiments of any of the above methods for generating ungulates or ungulate cells, an ungulate of the invention is mated with another ungulate to produce an embryo, fetus, or live offspring with two or more genetic modifications. Preferably, one or more cells are isolated from the embryo, fetus, or offspring, and one or more additional genetic modifications are introduced into the isolated cell(s).
Methods for Producing Antibodies
The invention also provides method for producing antibodies using an ungulate of the invention that expresses xenogenous antibodies (e.g., human antibodies). One such method involves administering one or more antigens of interest to an ungulate of the invention having nucleic acid encoding a xenogenous antibody gene locus. The nucleic acid segments in the gene locus undergo rearrangement resulting in the production of antibodies specific for the antigen. Antibodies are recovered from the ungulate. The antibodies may be monoclonal or polyclonal and are preferably reactive with an antigen of interest. Preferably, the antibodies are recovered from the serum or milk of the ungulate.
In a related aspect, the invention provides another method for producing antibodies that involves recovering xenogenous antibodies from an ungulate of the invention having nucleic acid encoding a xenogenous antibody gene locus. The nucleic acid segments in the gene locus undergo rearrangement resulting in the production of xenogenous antibodies. The antibodies may be monoclonal or polyclonal and are preferably reactive with an antigen of interest. Preferably, the antibodies are recovered from the serum or milk of the ungulate. Preferably, the ungulate antiserum or milk has polyclonal human immunoglobulins. Preferably, the antiserum or milk is from a bovine, ovine, porcine, or caprine. In another preferred embodiment, the immunoglobulins are directed against a desired antigen. In preferred embodiments, the antiserum is used as intravenous immunoglobulin (IVIG) for the treatment or prevention of disease in humans. In another preferred embodiment, an antigen of interest is administered to the ungulate, and immunoglobulins directed against the antigen are produced by the ungulate. Preferably, the nucleic acid segments in the xenogenous immunoglobulin gene locus rearrange, and xenogenous antibodies reactive with the antigen of interest are produced. Preferably, the antiserum and/or milk contains at least 2, 5, 10, 20, or 50 fold more xenogenous antibody than endogenous antibody, or contains no endogenous antibody. If desired, hybridomas and monoclonal antibodies can be produced using xenogenous B-cells derived from the above-described transgenic ungulates (for example, transgenic bovines). It is also contemplated that xenogenous antibodies (e.g., human antibodies) isolated from ungulates may be subsequently chemically modified so that they are covalently linked to a toxin, therapeutically active compound, enzyme, cytokine, radiolabel, fluorescent label, or affinity tag. If desired, the fluorescent or radiolabel may be used for imaging of the antibody in vitro or in vivo.
Ungulates and Donor Cells
Ungulates include members of the orders Perissodactyla and Artiodactyla, such as any member of the genus Bos. Other preferred ungulates include sheep, big-horn sheep, goats, buffalos, antelopes, oxen, horses, donkeys, mule, deer, elk, caribou, water buffalo, camels, llama, alpaca, pigs, and elephants.
Preferred cells for gene targeting include differentiated cells such as epithelial cells, neural cells, epidermal cells, keratinocytes, hematopoietic cells, melanocytes, chondrocytes, B-lymphocytes, T-lymphocytes, erythrocytes, macrophages, monocytes, fibroblasts, and muscle cells; and undifferentiated cells such as embryonic cells (e.g., stem cells and embryonic germ cells). In another preferred embodiment, the cell is from the female reproductive system, such as a mammary gland, ovarian cumulus, granulosa, or oviductal cell. Preferred cells also include those from any organ, such as the bladder, brain, esophagus, fallopian tube, heart, intestines, gallbladder, kidney, liver, lung, ovaries, pancreas, prostate, spinal cord, spleen, stomach, testes, thymus, thyroid, trachea, ureter, urethra, and uterus. Preferably, the donor cell, donor nucleus, donor chromatin mass, or reconstituted oocyte is not tetraploid.
Transgenic Ungulate Cells
In one aspect, the invention provides an ungulate cell (e.g., bovine cell) having a mutation (e.g., a mutation after the initial ATC codon, such as a mutation that is within 10, 20, 50, or 100 nucleotides of this codon) in one or both alleles of at least two genes encoding IgM heavy chain. Preferably, the mutations reduce or substantially eliminate the expression of functional IgM protein. In preferred embodiments, expression of functional or total IgM protein is decreased by at least 10, 20, 40, 60, 80, 90, 95, or 100%. The mutations may be hemizygous or homozygous. In some embodiments, the mutations include an insertion of a positive selection marker (e.g., an antibiotic resistance gene) into the nucleic acid. Preferably, the positive selection marker is operably linked to a xenogenous promoter. For ungulates or ungulate cells with an antibiotic resistance gene inserted into both alleles of a gene encoding IgM heavy chain, each allele may contain the same or a different antibiotic resistance gene. In a preferred embodiment, a negative selection marker (e.g., DT-A or Tk) is operably linked to a xenogenous promoter and is present in a vector used to disrupt an endogenous allele. The mutation may or may not include the deletion of one or more nucleotides (e.g., contiguous nucleotides) in the gene.
In preferred embodiments of the above aspect, the ungulate (e.g., bovine) or ungulate cell (e.g., bovine cell) has one or more transgenes and expresses an mRNA or protein (e.g., antibody) encoded by the transgene(s). Preferred ungulates contain naturally arranged segments of human chromosomes (e.g., human chromosomal fragments) or artificial chromosomes that comprise artificially engineered human chromosome fragments (i.e., the fragments may be rearranged relative to the human genome). In some embodiments, the xenogenous nucleic acid is contained within a chromosome fragment. The nucleic acid may be integrated into a chromosome of the ungulate or maintained in the ungulate cell independently from the host chromosome. In various embodiments, the nucleic acid is contained in a chromosome fragment, such as a ΔHAC, ΔΔHAC, or κHAC. In other embodiments, the xenogenous antibody is an antibody from another genus, such as a human antibody.
Preferred ungulates and ungulate cells have one or more nucleic acids having a xenogenous antibody gene locus (e.g., a nucleic acid encoding all or part of a xenogenous immunoglobulin (Ig) gene that undergoes rearrangement and expresses at least one xenogenous Ig molecule) in one or more B-cells. Preferably, the nucleic acid has unrearranged antibody light chain nucleic acid segments in which all of the nucleic acid segments encoding a V gene segment are separated from all of the nucleic acid segments encoding a J gene segment by one or more nucleotides. Other preferred nucleic acids have unrearranged antibody heavy chain nucleic acid segments in which either (i) all of the nucleic acid segments encoding a V gene segment are separated from all of the nucleic acid segments encoding a D gene segment by one or more nucleotides and/or (ii) all of the nucleic acid segments encoding a D gene segment are separated from all of the nucleic acid segments encoding a J gene segment by one or more nucleotides. Other preferred ungulates have one or more nucleic acids encoding all or part of a rearranged xenogenous immunoglobulin gene that expresses at least one xenogenous immunoglobulin.
In other preferred embodiments, the light chain and/or heavy chain of the xenogenous antibodies is encoded by a human nucleic acid. In preferred embodiments, the heavy chain is any class of heavy chain, such as μ, γ, δ, ε, or α, and the light chain is a lambda or kappa light chain. In other preferred embodiments, the nucleic acid encoding the xenogenous immunoglobulin chain or antibody is in its unrearranged form. In other preferred embodiments, more than one class of xenogenous antibody is produced by the ungulate. In various embodiments, more than one different xenogenous Ig or antibody is produced by the ungulate. The xenogenous antibody may be a polyclonal or monoclonal antibody.
Preferably, the ungulate also has a mutation in one or both alleles of an endogenous nucleic acid encoding prion protein, alpha-(1,3)-galactosyltransferase and/or J chain. Preferably, the mutation reduces or eliminates the expression of the endogenous alpha-(1,3)-galactosyltransferase enzyme, galactosyl(α1,3)galactose epitope, and/or J chain. Preferably, the ungulate produces human IgA or IgM molecules containing human J chain. Preferred ungulate cells (e.g., bovine cells) include somatic cells, such as fetal fibroblasts or B-cells.
The process of producing a transgenic ungulate of the invention involves the mutation (e.g., by homologous recombination) of one or both alleles of at least two IgM heavy chain genes (e.g., bovine IgμU and IgμAY genes). Gene mutation may be effected by homologous recombination. In a preferred embodiment, fetal fibroblasts are targeted in vitro using a suitable homologous recombination vector. The use of fetal fibroblasts is preferred over some other somatic cells as these cells are readily propagated and genetically manipulated in tissue culture. However, the use of fetal fibroblasts is not essential to the invention, and other cells may be substituted therefor with equivalent results. Suitable somatic cells include fibroblasts, epithelial cells, endothelial cells, neural cells, keratinocytes, hematopoietic cells, melanocytes, chondrocytes, lymphocytes (B-cells and T-cells), macrophages, monocytes, mononuclear cells, cardiac muscle cells, other muscle cells, granulosa cells, cumulus cells, placental cells, and epidermal cells.
Targeted gene mutation requires constructing a DNA construct having regions of homology to the targeted IgM heavy chain allele such that the construct upon integration into an IgM heavy chain allele in the ungulate genome disrupts the expression thereof. Exemplary vectors for carrying out such targeted mutation of bovine IgμU and IgμAY are described in the examples that follow. Methods for constructing vectors that provide for homologous recombination at other targeted sites are well known to those skilled in the art. Moreover, in the present instance, the construction of a suitable vector is within the level of skill in the art, given especially that the sequences of Igμ genes from other ungulates (e.g., sheep and goats) are known (see below). In order to facilitate homologous recombination, the vectors used to effect homologous recombination and inactivation of the IgM gene, respectively, comprise portions of DNA that exhibit substantial sequence identity to the ungulate IgM heavy and Ig light chain genes. Preferably, these sequences possess at least 98% sequence identity, more preferably, at least 99% sequence identity, and still more preferably are isogenic with the targeted gene loci to facilitate homologous recombination and targeted deletion or inactivation.
Typically, the construct includes a marker gene that allows for selection of desired homologous recombinants, for example, fibroblasts, wherein the IgM heavy chain gene has been disrupted by homologous recombination. Exemplary marker genes include antibiotic resistance markers, drug resistance markers, and green fluorescent protein, among others.
One neomycin resistance construct was assembled as follows. A construct designated “pSTneoB” (Katoh et al. (1987) Cell Struct. Funct. 12:575; Japanese Collection of Research Biologicals (JCRB) deposit number: VE039) was designed to contain a neomycin resistance gene under the control of an SV40 promoter and TK enhancer upstream of the coding region. Downstream of the coding region is an SV40 terminator sequence. The neo cassette was excised from “pSTneoB” as a XhoI fragment. After the ends of the fragment were converted to blunt ends using standard molecular biology techniques, the blunt ended fragment was cloned into the EcoRV site in the vector, pBS246 (Gibco/Life Technologies). This site is flanked by loxP sites. The new construct, designated “pLoxP-STNeoR”, was used to generate the mu knockout DNA construct. The desired fragment of this construct is flanked by loxP sites and NotI sites, which were originally present in the pBS246 cloning vector. The desired NotI fragment, which contains loxP-neo-loxP, was used for replacement of the immunoglobulin mu constant region exons. The SV40 promoter operably linked to the neomycin resistance gene activates the transcription of the neomycin resistance gene, allowing cells in which the desired NotI fragment has replaced the mu constant region exons to be selected based on their resulting antibiotic resistance.
After a cell line is obtained in which an IgM heavy chain allele has been effectively disrupted, it is used as a donor cell to produce a cloned ungulate fetus (for example, a cloned bovine fetus) and eventually a fetus or animal wherein one of the IgM heavy alleles is disrupted. Thereafter, a second round of gene targeted mutation can be effected using somatic cells (e.g., fibroblasts) derived from the fetus or animal to produce cells in which a second IgM heavy chain allele is disrupted.
The following examples are meant to illustrate the invention. They are not meant to limit the invention in any way.
We have developed a broadly applicable and rapid method for generating multiple gene targeting events. As discussed above, sequential application of a highly efficient targeting system and rejuvenation of cell lines by production of cloned fetuses (
Three of six fetuses (#2184-1, #2184-2 and #3287) were heterozygous KOs (IgμU−/+;
Non-targeted fetuses likely resulted from non-targeted cells that co-existed with the targeted cells in the wells. Both #2184-1 and #2184-2 were derived from well #384 where the KO vector was integrated into allele A, and fetus #3287 was from well #147 where the KO vector was integrated into allele B. Cloned IgμU−/+ embryos produced from all three regenerated cell lines were transferred to 153 recipients to produce 13 (8%) healthy IgμU−/+ calves, confirmed by PCR (
All three IgμU−/+ cell lines (#2184-1 and #2184-2, targeted in allele A; #3287, targeted in allele B) were used for targeting with the second KO vector (pBCμΔKOneo;
Two IgμU−/− wells (#76, #91), derived from cell line #3287, were selected for embryonic cloning to generate fetuses and rejuvenate the cell lines. Overall, the pregnancy rate for IgμU−/− fetuses at 40 to 50 days of gestation was 45% (40/89). At 45 days of gestation, five fetuses derived from well #76 and 15 fetuses from well #91 were collected and evaluated. All five from well #76 (
Two calves from this group were born recently and were confirmed to be IgμU−/− by PCR (
Sequential targeting requires a strategy for antibiotic selection of a newly integrated targeting vector in a cell line that already contains one or multiple antibiotic selection markers. The simplest approach is to use a different selection marker gene for each targeting event. This approach however, limits the number of targeting events that may be performed in a cell line. Another approach is to remove the selection markers using a Cre-loxP recombination system, as has been done in murine embryonic stem cells (Abuin and Bradley (1996) Mol. Cell Biol. 16:1851-1856). In our regenerated IgμU targeted fibroblasts, the selection marker genes were not expressed, likely because the IgμU locus is silent in fibroblasts. Although selection marker removal was not necessary for further targeting in our IgμU−/− fibroblasts, we evaluated the possibility of removing the selection markers by transfection with a Cre recombinase expression plasmid. Because the intention was for transient expression of Cre recombinase, a closed circular plasmid was used and antibiotic selection was restricted to the first three days of culture. Bovine IgμU−/− cell line #4658 was used for transfection and 24 selected wells were evaluated by PCR for excision of the antibiotic selection genes from the targeted alleles (
Five fetuses were recovered and all had both selection markers removed (
To evaluate the possibility of sequentially targeting a second gene, Cre-excised IgμU−/− (Cre/IgμU−/−) fibroblasts (cell line #1404) were subjected to a third round of targeting to disrupt the PrP gene. This gene was first characterized to identify a polymorphic sequence, outside the KO vector sequence, to distinguish the two alleles (
Thirteen (6.4%) wells with cells showing a heterozygous KO PrP in Cre/IgμU−/− background (Cre/IgμU−/−/PrP−/+) were identified (primer pairs; neoF7×neoR7;
No amplification was detected following negative IgμU PCR, as expected (
To examine the feasibility of quadruple targeting to produce double homozygous KO fetuses and cell lines, the triple targeted cell line (#8443, Cre/IgμU−/−/PrP−/+) was transfected with a fourth KO vector for the remaining allele of the PrP gene. The vector was constructed by replacing the neo gene with a puro gene (pBPrP(H)KOpuro,
Our results indicate that multiple rounds of gene targeting, both for active and silent genes, are readily accomplished in somatic cells using a cell rejuvenation approach. The system proved effective for targeting both transcriptionally silent and active genes, demonstrating broad application, and was compatible with development of healthy calves through at least two rounds of targeting. Furthermore, there is no indication that additional rounds of targeting compromised development of cloned embryos.
Methods
The results described in Examples 1-3 were obtained using the following methods.
Construction of KO Vectors
A bovine genomic fragment around exon 2 of the IgμU constant region locus was obtained from non-isogenic Holstein genomic library by probing with a 32P-labeled PCR fragment amplified with primer pair: 5′-TGGTCACTCCAAGT GAGTCG-3′ (SEQ ID NO: 1) and 5′-TGGAGTGAAATCAGGTGAAGG-3′ (SEQ ID NO: 2). One genomic clone was, analyzed further by restriction mapping. The 7.2 kb of BglII-XhoI genomic fragment (5′ homologous arm) and 2.0 kb of BamHI-BglII fragment (3′ homologous arm) around the exon 2 were subcloned into pBluescript II SK(−) (Stratagene), and then puro, STOP cassettes (pBS302, Stratagene) and DT-A (diphtheria toxin A) genes were inserted (pBCμΔKopuro). For construction of the second targeting vector, genomic PCR was performed from #6939 by using primer pair: 5′-GCAATAGCAAGTCCAGC CTCATCTG-3′ (SEQ ID NO: 3) and 5′-CATCCTGTCTCTGGTGGTTTGAGG TC-3′ (SEQ ID NO: 4). After digestion with BamHI-BglII, the fragment replaced the 3′ short arm of the pBCμΔKOpuro vector. By sequencing, the BamHI-BglII fragment was confirmed to be amplified from “allele A”.
The puro gene was replaced with a neo gene (pBCμΔKOneo vector). Bovine genomic fragment around exon 3 of the PrP locus was obtained by screening of the same Holstein genomic λ phage library with a 32P-labeled DNA fragment amplified by PCR primer pair: 5′-GATTGAATGGTCTCCAGGATG CC-3′ (SEQ ID NO: 5) and 5′-GACAAGCTTAATATCCGCAGG-3′ (SEQ ID NO: 6). One genomic clone was analyzed further by restriction mapping. The 8.3 kb of Bam HI genomic fragment (3′ homologous arm) and 1.2 kb of BamHI-BglII fragment (5′ homologous arm) containing exon 3 were subcloned into pBluescript II SK(−), and then both neo and STOP cassettes were inserted at the BamHI site, which is behind the initial ATG codon. The DT-A gene was also subcloned (pBPrP(H)KOneo vector). Similarly, another KO vector containing the puro gene was constructed (pBPrP(H)KOpuro vector).
Cell Culture and Transfection
Holstein fetal male fibroblasts were cultured as previously described (Kuroiwa et al. supra) and electroporated with 30 μg of each targeting vector at 550 V and 50 μF by using a GenePulser II (Bio-rad). After 48 hours, the cells were selected under 500 μg/ml of G418 or 1 μg/ml of puromycin for two weeks and the drug-resistant colonies were picked and transferred to replica plates; one for genomic DNA extraction (24-well plates) and the other for embryonic cloning (48-well plates).
Genomic PCR Analyses
From the replica 24-well plates, fetus or ear biopsy genomic DNA from calves was extracted using a Puregene DNA extraction kit (GentraSystem). To identify each homologous recombination event that occurred at IgμU targeting, puroF2 (5′-GAGCTGCAAGAACTCTTCCTCACGC-3′, SEQ ID NO: 7), puroR2 (5′-ATGTACCTCCCAGCTGAGACAGAGGG-3′, SEQ ID NO: 8), neoF3 (5′-TTTGGTCCTGTAGTTTGCTAACACACCC-3′, SEQ ID NO: 9) and neoR3 (5′-GGATCAGTGCCTATCACTCCAGGTTG-3′, SEQ ID NO: 10) primer pairs were used. PCR was performed in 30 cycles comprising 98° C.-10 s, 68° C.-8 min. For negative PCR, BCμf (5′-TGGTCACTCCAAGTGAGTCG-3′, SEQ ID NO: 11) and BCμr (5′-TGGAGTGAAATCAGGTGAAGG-3′, SEQ ID NO: 12) were used in 40 cycles of PCR composed of 98° C.-10 s, 62° C.-30 s, 72° C.-1 min. In the case of the PrP locus, neoF7 (5′-TGTCAAAGAGACACTCCTCATTTGTCT TCC-3′, SEQ ID NO: 13), neoR7 (5′-TCATAGCCGAATAGCCTCTCCACCC-3′, SEQ ID NO: 14), puroF14 (5′-TTGCTCCACCTTCCGTCTTCTGTC-3′, SEQ ID NO: 15) and puroR14 (5′-GTTGGCGCCTACCGGTGGATGTTTG-3′, SEQ ID NO: 16) primer pairs were used. PCR was performed in 30 cycles comprising 98° C.-10 s, 68° C.-5 min. For negative PCR, BPrPexF (5′-CCACATAGGCAGTTG GATCC-3′, SEQ ID NO: 17) and BPrPexR (5′-ATAAGAGGCCTGCTCATG GC-3′, SEQ ID NO: 18) primer pairs were used in the 40 cycles of PCR composed of 98C-10 s, 62C-30 s, 72C-1 min. To detect the Cre-mediated excision, PCR was carried out with CreExF (5′-CAATAGCAAGTCCAGCCTCATCTGC-3′, SEQ ID NO: 19) and CreExR (5′-GTGGTTTCTTCGGTGGAAACAACG-3′, SEQ ID NO: 20) primer pair in 40 cycles of PCR composed of 98° C.-10 s, 68° C.-7 min. All the PCR products were run on 0.8% agarose gels.
Sequencing of PCR Products
To confirm whether homologous recombination correctly occurred at each targeting step, the PCR products amplified above were sequenced. The PCR products were purified through CHROMA SPIN-TE400 column (BD Biosciences Clontech) and sent to ACGT Inc. (Wheeling, Ill.) to sequence. Sequence was bi-directionally done both with forward and reverse primers which were used for PCR. The allele into which each KO vector was integrated was determined by polymorphisms in the sequence of the PCR products.
Permeabilized Cell Transfer
Cloned fetuses and calves were produced using a permeabilized cell transfer procedure as described previously (Sullivan et al. (2004) Biol. Reprod. 70:146-153). In vitro matured oocytes were enucleated at 20 h post maturation. Correctly targeted clones were permeabilized by incubation of about 50,000-100,000 cells in suspension with 31.2 U Streptolysin O (SLO; Sigma) in 100 μl HBSS for 30 min in a 37° C. H2O bath.
Permeabilized cells were sedimented, washed and incubated with 40 μl mitotic extract containing an ATP generating system (1 mM ATP, 10 mM creatine phosphate and 25 μg/ml creatine kinase) for 30 min at 38° C. At the end of the incubation, the reaction mix was diluted, sedimented and washed.
These cells were fused to enucleated oocytes, activated at 28 h post maturation with 5 μM calcium ionophore for 4 min followed by 10 μg/ml cycloheximide and 2.5 μg/ml cytochalasin D for 5 h. After activation, embryos were washed and co-cultured with mouse fetal fibroblasts to the blastocyst stage in vitro. Grade 1 and 2 blastocysts were selected and transferred into synchronized recipients. All animal work was done following a protocol approved by the Transova Genetics Institutional Animal Care and Use Committee.
RT-PCR
RNA was extracted from spleen of wild-type (#6939) and IgμU−/− fetuses using an RNeasy mini kit (Qiagen) and first strand cDNA synthesis was done using the superscript first strand synthesis system for RT-PCR (Invitrogen). PCR was done using BCμf (5′-TGGTCACTCCAAGTGAGTCG-3′, SEQ ID NO: 21) and BCμr (5′-TGGAGTGAAATCAGGTGAAGG-3′, SEQ ID NO: 22) primers in 40 cycles of PCR composed of 98° C.-10 s, 62° C.-30 s, 72° C., 1 min. RNA was also extracted from #4658 (IgμU−/−), #8443 (IgμU−/−/PrP−/+) and double homozygous KO (IgμU−/−/PrP−/−) fibroblasts and first strand cDNA synthesis was done as above. PCR was done by using PrPmF3 (5′-CAAAACCTGGAGGAGGATGG-3′, SEQ ID NO: 23) and PrPmR3 (5′-ATAAGAGGCCTGCTCATGGC-3′, SEQ ID NO: 24) primers in 40 cycles of 98° C.-10 s, 62° C.-30 s, 72° C.-1 min. For detection of bovine α-actin mRNA expression, bBAF (5′-ACATCCGCAAGGACCTCT AC-3′, SEQ ID NO: 25) and bBAR (5′-AACCGACTGCTGTCACCTTC-3′, SEQ ID NO: 26) primers were used in the same PCR condition. To exclude the possibility of genomic DNA contamination, another RT-PCR was performed without reverse-transcriptase. The PCR products were ran on 0.8% agarose gel.
During our analysis of IgμU−/− fibroblasts we identified expression of a transcript that could be detectable by primers designed to amplify IgμU−/−. To determine the identity of this transcript, total RNA was extracted by using RNeasy Mini kit (QIAGEN) from spleen of IgμU−/− fetuses collected at 90 days of gestation. One microliter of total RNA was subjected to first-strand cDNA synthesis (SuperScript First-Strand Synthesis System for RT-PCR, Invitrogen), followed by RT-PCR, which was carried out as below. The primer pair used was 5′-TGGTCACTCCAAGTGAGTCG-3′ (BCμf; SEQ ID NO: 27) and 5′-TGGAGTGAAATCAG GTGAAGG-3′ (BCμr; SEQ ID NO: 28). The PCR reaction mixtures contained 32.5 μl water, 5 μl of 10× Ex Taq buffer (TAKARA), 8 μl of dNTP mixture, 10 μmol forward primer, 10 μmol of reverse primer, 2 μl of the first-strand cDNA, and 0.5 μl of Ex Taq (TAKARA). Thirty five cycles of PCR were performed by incubating the reaction mixtures at the following conditions: 85° C. for three minutes, 94° C. for one minute, 98° C. for 10 seconds, 62° C. for 30 seconds and 72° C. for 1 minute. After PCR, the reaction mixtures were analyzed by electrophoresis. There were no positive PCR products in the IgμU−/− fetuses. However, when another primer pair 5′-TCTCTGGTGACGGC AATAGC-3′ (BCμf2; SEQ ID NO: 30) and 5′-CTTCGTGAGGAAGATGTC GG-3′ (BCμr2; SEQ ID NO: 31) was used for RT-PCR, a positive PCR product was detected in the IgμU−/− fetuses (
To determine whether both IgμAY and IgμU genes are expressed following VDJ rearrangement, mRNA was similarly extracted from spleens of #6939 (original cell line)-derived and IgμU−/− fetuses. RT-PCR was performed using 5′-CCCTCCTCTTTGTGCTGTCA-3′ (BL17; SEQ ID NO: 32) and 5′-GTTCA GGCCATCATAGGAGG-3′ (mBCμ-R2; SEQ ID NO: 33) in thirty five cycles composed of conditions: 85° C. for three minutes, 94° C. for one minute, 98° C. for 10 seconds, 62° C. for 30 seconds and 72° C. for 1 minute. The PCR products were purified through CHROMA SPIN TE-100 column (BD) and sent to ACGT Inc. to directly sequence them with mBCμ-R2 primer. According to sequence peak chart, IgμAY transcript was mainly expressed, in addition to a very small amount of that of IgμU in #6939 fetuses, revealing that both IgμAY and IgμU underwent VDJ rearrangement to be expressed and that both of them are functional in terms of transcription (
IgμAY KO vectors were generated as follows. To isolate genomic DNA around exon 2 of the IgμAY gene, a DNA probe was amplified by PCR using 5′-TCTCTGGTGACGGCAATAGC-3′ (SEQ ID NO: 34) and 5′-CTTCGTGAGGA AGATGTCGG-3′ (SEQ ID NO: 35) (BCμ-f2 and BCμ-r2). Using this probe, a bovine (Holstein) genomic λ phage library derived from #4658 IgμU homozygous KO cell line was screened, and 83 positive λ phage clones were identified. These clones should contain both alleles of intact IgμAY gene and both alleles of targeted IgμU gene. To distinguish intact IgμAY clones from the targeted IgμU clones, λDNA isolated from each clone was subjected to PCR using primer pair BCμ-f2 and BCμ-r2. In the case of clones containing the targeted IgμU gene, the PCR product cannot be amplified because of presence of the KO cassette integrated at exon 2. On the other hand, the PCR product can be amplified from intact IgμAY locus; clones producing the PCR product should be ones including intact IgμAY gene, but clones not producing the PCR products should be ones including the targeted IgμU gene. Out of 83 λ phage clones, 26 produced the PCR products and these were confirmed to be clones containing intact IgμAY gene by sequence (primer AYU-F2; 5′-GGCTGACTCCCTACCTCCCCTACAC-3′ (SEQ ID NO: 36). At least other 10 clones that did not produce the PCR products proved to contain the targeted IgμU gene, confirmed by sequence (primer AYU-F2). The foregoing demonstrated that there are at least two Igμ genes in bovine, and that one gene (which we refer to as IgμU) is disrupted in our KO cell line (#4658) but the other gene (IgμAY) is still intact (
To distinguish both alleles of IgμAY, we sequenced all the λ phage DNA using primer AYU5′ (5′-CGGAGCCCCTGGAGATGAGC-3′) (SEQ ID NO: 37). According to this sequencing, we found polymorphic sequences to differentiate the alleles of IgμAY gene, which we named the AY allele and ay allele (
Transfection of IgμU homozygous KO cell lines with IgμAY KO vectors was performed using the following standard electroporation protocol. The medium used to culture the bovine fetal fibroblasts contained 500 ml alpha MEM (Gibco, 12561-049), 50 ml fetal calf serum (Hy-Clone #ABL13080), 5 ml penicillin-streptomycin (SIGMA), and 1 ml 2-mercaptoethanol (Gibco/BRL #21985-023). On the day prior to transfection, cells were seeded on a T175 tissue culture flask with a confluency of 80-100%, as determined by microscopic examination. On the day of transfection, about 1 bovine fibroblasts cells were trypsinized and washed once with alpha-MEM medium. After resuspension of the cells in 800 μl of alpha-MEM, 30 μg of the SrfI-digested KO vector (pBCμAYKObsr vector) dissolved in HEPES buffer saline (HBS) containing 1 mM spermidine was added to the cell suspension and mixed well by pipetting. The cell-DNA suspension was transferred into an electroporation cuvette and electroporated at 550 V and 50 μF. After that, the electroporated cells were plated onto thirty 48-well plates with the alpha-MEM medium supplemented with the serum. After a 48 hour-culture, the medium was replaced with medium containing 10 μg/ml of blasticidine, and the cells were cultured for 2-3 weeks to select blasticidine resistant cells. After selection, all colonies which reached close to 100% confluency were divided into two replica plates (24-well and 48-well plates): one for genomic DNA extraction, and the other plate for embryo cloning. Genomic DNA was extracted from the colonies to screen for the desired homologous recombination events by PCR.
Genomic DNA was independently extracted from each 24-well using the PUREGENE DNA isolation Kit (Gentra SYSTEMS) according to the manufacturer's protocol. Each genomic DNA sample was resuspended in 20 μl of 10 mM Tris-Cl (pH 8.0) and 1 mM EDTA (EDTA). Screening by PCR was performed using the following primer pair AYKObsrF2 (5′-GGTAGTGCAGT TTCGAATGGACAAAAGG-3′; SEQ ID NO: 38) and AYKObsrR2 (5′-TCAGG ATTTGCAGCACACAGGAGTG-3′; SEQ ID NO: 39). The sequence of one primer is located in the KO vector, and the sequence of the other primer is located just outside of the integrated vector in the targeted endogenous locus. Therefore, the expected PCR product is detected only when the KO vector is integrated into the targeted locus by homologous recombination. The PCR reaction mixtures contained 17.9 μl water, 3 μl of 10× LA PCR buffer II (Mg2+ plus), 4.8 μl of dNTP mixture, 10 μmol of forward primer, 10 μmol of reverse primer, 2 μl of genomic DNA, and 0.3 μl of LA Taq. Forty cycles of PCR were performed by incubating the reaction mixtures under the following conditions: 85° C. for three minutes, 94° C. for one minute, 98° C. for 10 seconds, and 68° C. for 8 minutes. After PCR, the reaction mixtures were analyzed by electrophoresis. Out of 322 screened clones, 22 clones generated the expected PCR products. As a result of sequencing of the PCR products, the KO vector designed to target AY allele was exclusively integrated into the AY allele in all the clones.
pBCμayKOhyg vector also was transfected to IgμU homozygous KO cell lines, except that the vector was digested with SalI before electroporation. As a result of screening of 453 hygromycin-resistant colonies, 29 clones were identified as a positive by PCR using the following primer pair ayKOhygF2 (5′-TGGTTGG CTTGTATGGAGCAGCAGAC-3′; SEQ ID NO: 40) and ayKOhygR2 (5′-TAGG ATATGCAGCACACAGGAGTGTGG-3′; SEQ ID NO: 41). Sequencing of the PCR products demonstrated that the KO vector designed to target ay allele was exclusively integrated into the ay allele. Judging from the above results, it can be said that both the AY and ay KO vectors specifically target each allele in an allele-specific manner and produce correct targeted clones at a frequency of 7-8%. Chromatin transfer was performed as follows. In vitro-matured oocytes were enucleated at 20 hpm. Bovine IgμU knockout fibroblasts were trypsinized and washed in Ca/Mg Hank's Balanced Salt Solution (HBSS) and permeabilized by incubation of 50,000-100,000 cells in 31.25 units Streptolysin O (SLO; Sigma, St. Louis, Mo.) in 100 μl for 30 minutes in a 37° C. H2O bath. Cell samples were incubated with propidium iodide and observed by fluorescent microscopy to monitor permeabilization based on uptake of the dye. Permeabilized fibroblasts were washed, pelleted, and incubated in 40 μl of mitotic extract prepared from MDBK cells containing an ATP-generating system (1 mM ATP, 10 mM creatine phosphate, and 25 μg/ml creatine kinase) for 30 minutes in a 37° C. H2O bath. Cell samples were stained with Hoechst 33342 and observed by florescent microscopy to monitor chromatin condensation. At the end of incubation, the reaction mix was diluted with 500 μl cell culture media (alpha MEM with 10% FBS). These cells were pelleted and resuspended in TL HEPES and used for chromatin transfer in enucleated oocytes. Twelve fetuses were determined to be hemizygous IgμAY KO fetuses in which the bsrKO vector is integrated into AY allele of the IgμAY gene. Likewise, eleven fetuses were determined to be hemizygous IgμAY KO fetuses in which the hygKO vector is integrated into ay allele of the IgμAY gene. These fetuses were also IgμU−/−. One of the IgμAY−/+/IgμU−/− cell lines (A227, targeted in AY allele) was used for a second targeting experiment with pBCμayKOhyg. As a result of screening of 197 hygromycin-resistant colonies, 18 clones were identified as a positive by PCR using the primer pair (ayKOhygF2 and ayKOhygR2). Sequencing of the PCR products demonstrated that the KO vector designed to target ay allele was exclusively integrated into the ay allele, producing double homozygous knockout (IgμAY−/−/IgμU−/−) cells.
We produced IgμAY−/−/IgμU−/− fetuses using the methods described herein. To examine whether these fetuses were B cell-deficient, we collected IgμAY−/−/IgμU−/− fetuses at 180 days of gestation. From spleen, total RNA was extracted by using RNeasy Mini kit (QIAGEN). One microliter of total RNA was subjected to first-strand cDNA synthesis (SuperScript First-Strand Synthesis System for RT-PCR, Invitrogen), followed by RT-PCR. One RT-PCR reaction was carried out as below. The primer pair used (5′-CCCTCCTCTTTGTGCTGTCA-3′ (BL17; SEQ ID NO: 42) and 5′-GTTCAGGCCATCATAGGAGG-3′ (nBCpR2; SEQ ID NO: 43)) is compatible both with IgμAY and IgμU amplification. The PCR reaction mixtures contained 32.5 μl water, 5 μl of 10× Ex Taq buffer (TAKARA), 8 μl of dNTP mixture, 10 μmol forward primer, 10 μmol of reverse primer, 2 μl of the first-strand cDNA, and 0.5 μl of Ex Taq (TAKARA). Thirty five cycles of PCR were performed by incubating the reaction mixtures at the following conditions: 85° C. for three minutes, 94° C. for one minute, 98° C. for 10 seconds, 62° C. for 30 seconds and 72° C. for 1 minute. After PCR, the reaction mixtures were analyzed by electrophoresis. There were no positive PCR products in the IgμAY−/−/IgμU−/− fetuses. After PCR, the reaction mixtures were analyzed by electrophoresis. No expression of IgμAY or IgμU could be detected. We also performed flow cytometry analysis to detect the presence of IgM heavy chain protein. No such protein could be detected.
To determine if the IgμU gene can, by itself, support B cell development, we generated IgμAY knockouts similarly as described above by using pbCFμayKOhyg and pbCμAYKObsr vectors. From one IgμAY−/− cell line, we generated 180 day fetuses and performed RT-PCR on spleen tissue in an attempt to detect VDJ-rearranged IgμU transcripts. Sequence of the RT-PCR products showed VDJ-rearranged sequence of IgμU transcripts, exclusively, and disruption of IgμAY gene expression was confirmed. We confirmed, from sequence analysis, the presence of possible VH and DH segments associated with JH-CμL segments, which comprise the corresponding FR1, CDR1, FR2, CDR2, FR3 and CDR3 regions. Furthermore, direct sequencing of the RT-PCR products clearly showed diversified sequence in the CDR3 region of IgμU transcripts as well as that of IgμAY. The sequence of IgμU cDNA appears to encode functional IgM-like polypeptides, which are slightly different from IgμAY, especially in FR4 and the constant region. These data demonstrate that the IgμU gene can be expressed independently of IgμAY expression, following functional VDJ-rearrangement, which included diversification of CDR3.
To investigate whether functional expression of IgμU could sufficiently execute B cell development independently of the classical pathway through IgμAY, we performed flow cytometry analysis in IgμAY−/− 180 day fetuses. We recognized significant populations of developing B cells (IgM+B220+), comparable to controls. This result indicates that IgμU protein can be displayed on the B cell surface. Next, to investigate generation of immature B cells (IgM+Igλ+), staining was done with both an anti-bovine IgM polyclonal antibody and an anti-bovine Igλ monoclonal antibody. Double positive B cells were detected, suggesting the presence of immature B cells where IgμU heavy chain couples with Igλ light chain to form B cell-receptors. Furthermore, we detected generation of mature B cells, recognized by anti-CD21 antibody, and confirmed gene expression of VDJ-rearranged bIgD gene by RT-PCR. This observation suggests that IgμU can support mature B cell development and elicit the expression of a VDJ-Cδ gene. Moreover, we detected the occurrence of class switching and expression of VDJ-rearranged bIgG, even though the γ constant region segment has not been identified as part of the IgμU gene cluster. Interestingly, the extent of diversification of CDR3 in the bIgG transcripts, from IgμAY−/− fetuses, was significantly less than that detected in IgμU−/− fetuses. However, CDR3 in IgμU transcripts in IgμAY−/− fetuses was diversified at a level comparable to that of IgμAY transcripts in IgμU fetuses. This result suggests that IgμU may be less efficient at generation of well-diversified IgG compared to the classical IgμAY locus, probably because the IgμU locus does not link, in cis, with the bIgG constant region. These data strongly suggest that IgμU can largely substitute for lack of IgμAY function in driving B cell development, but likely recruits y constant region segments from the IgμAY locus by some trans-class switch mechanism.
From these results, it is demonstrated that there are two functional IgM loci, IgμAY and IgμU, in bovine and that double homozygous knockouts IgμAY−/−/IgμU−/− are useful for B cell-deficiency.
The present invention features the production of bovines that are deficient in prion protein expression. Although PrP−/− mice are viable and appear healthy, in cattle the effect of knocking out the gene had not previously been determined. In an attempt to generate PrP−/− cattle, we exploited a sequential gene targeting system for bovine fibroblasts described above. Male Holstein fibroblasts (cell line 4685, which is also IgμU−/−) were transfected with a knockout vector (pBPrP(H)KOneo), and correctly targeted cells were cloned to generate PrP−/+ fetuses at 40 days of gestation that were used to establish PrP−/+ cell lines. Fetal cell lines were evaluated to confirm correct targeting by PCR genotyping. The PrP−/+ cell line was then transfected with a second knockout vector (pBPrP(H)KOpuro) followed by selection and cloning to generate PrP−/− fetuses and cell lines. Correct targeting in cell lines was verified by PCR genotyping. Wild type, PrP+/+/IgμU−/− (cell line 5112), heterozygous knockout, PrP−/+IgμU−/− (cell line 8018), and homozygous knockout, PrP−/−/IgμU−/− (cell line 1718) fibroblasts were used for embryonic cloning to produce calves (Table 4). In our first cloning series we obtained one calf each from PrP+/+/IgμU−/− (calf 347; 5.9%), PrP−/+/IgμU−/− (calf 341; 4.3%) and PrP−/−/IgμU−/− (calf 342; 3.2%) cell lines (
In order to verify the genotype of calves 341 and 342, we collected a small biopsy of skin from each calf and established fibroblast cell lines (
To demonstrate functional inactivation of the PrP gene in calf 342, we extracted mRNA and total protein from the fibroblasts. As controls, we analyzed PrP+/+/IgμU−/− calf 347 and PrP−/+/IgμU−/− calf 341. For mRNA expression analysis, we performed RT-PCR (primer pairs; PrPmF3×PrPmR3,
All calves have been evaluated by either a licensed veterinarian or trained animal care technician. At one week and one month, calves 341 and 342, along with controls, were given physicals which included evaluation of the following parameters: body weight, body temperature, heart rate, heart sound, jugular vein distension, respiratory rate, respiratory sound, cough, presence of nasal discharge or eye abnormalities, appetite, general behavior (alert and active, sluggish, hyperactive), gait, posture, joints, hooves, feces (diarrhea, constipation), genitalia, and umbilical cord (dry, enlarged, inflamed, infected). In addition, blood samples were taken for standard hematology and serum chemistry. To date all parameters for calves 341 and 342 have been normal for these age groups.
For added verification, a second set of cloned PrP−/− embryos, which were only PrP−/− without any other genetic modification, were produced and transferred into 51 recipients. From these transfers, seven calves (14%) have been born (Table 5). These calves were verified by PCR genotyping and western blot analysis of peripheral blood lymphocytes (PBLs) isolated from blood biopsy (
Methods
The results described in Example 6 were obtained using the following methods.
Embryonic Cloning
Cloned calves were produced using a chromatin transfer procedure. In vitro matured oocytes were enucleated at 20 h post maturation. Correctly targeted clones were permeabilized by incubation of about 50,000-100,000 cells in suspension with 31.2 U Streptolysin 0 (SLO; Sigma) in 100 μl HBSS for 30 min in a 37° C. H2O bath. Permeabilized cells were sedimented, washed, and incubated with 40 μl mitotic extract containing an ATP generating system (1 mM ATP, 10 mM creatine phosphate and 25 μg/ml creatine kinase) for 30 min at 38° C. At the end of the incubation, the reaction mix was diluted, sedimented, and washed. These cells were fused to enucleated oocytes, activated at 28 h post maturation with 5 μM calcium ionophore for 4 min, followed by 10/g/ml cycloheximide and 2.5 μg cytochalasin D for 5 h. After activation, CT embryos were washed and co-cultured with mouse fetal fibroblasts to blastocyst stage in vitro. Grade 1 and 2 blastocysts were selected and transferred into synchronized recipients. All animal work described in this section was done following a protocol approved by the Transova Genetics institutional animal care and use committee.
Cell Culture and Transfection
Holstein fetal male fibroblasts were cultured and electroporated with 30 μg of each knockout vector at 550 V and 50 μF using a GenePulser II (Bio-Rad). After 48 hours, the cells were selected using 500 μg/ml G418 or 1 μg/ml puromycin for two weeks, and the drug-resistant colonies were picked and transferred to replica plates; one for genomic DNA extraction (24-well plates) and the other for embryonic cloning (48-well plates).
Genomic PCR Analyses
Genomic DNA was extracted from fibroblasts originated from either ear biopsy or blood of PrP−/− homozygous KO calves using a PUREGENE DNA isolation Kit (Gentra SYSTEMS) and the manufacture's protocol. Each genomic DNA sample was resuspended in 50-100 μl of 10 mM Tris-Cl (pH 8.0) and 1 mM EDTA (EDTA). Confirmation by PCR was performed using the following primer pairs neoF7; 5′-TGTCAAAGAGACACTCCTCATTTGTCTTCC-3′ (SEQ ID NO: 44) and neoR7; 5′-TCATAGCCGAATAGCCTCTCCACCC-3′ (SEQ ID NO: 45) to detect the first targeted allele, and puroF14; 5′-TTGCTCCACCTTCC GTCTTCTGTC-3′ (SEQ ID NO: 46) and puroR14; 5′-GTTGGCGCCTACCGG TGGATGTG-3′ (SEQ ID NO: 47) to detect the second targeted allele. The PCR reaction mixtures contained 17.9 μl water, 3 μl of 10×LA PCR buffer II (Mg2+ plus), 4.8 μl of dNTP mixture, 10 μmol forward primer, 10 μmol of reverse primer, 2 μl of genomic DNA, and 0.3 μl of LA Taq. Thirty cycles of PCR were performed by incubating the reaction mixtures at the following conditions: 85° C. for three minutes, 94° C. for one minute, 98° C. for 10 seconds, and 68° C. for 5 minutes. In addition, another PCR was performed to confirm the absence of (i) wild type PrP alleles using the following primer pairs: BPrPex3F; 5′-CCACATA GGCAGTTGGATCC-3′ (SEQ ID NO: 48) and BPrPex3R; 5′-ATAAGAGGCC TGCTCATGGC-3′ (SEQ ID NO: 49) and (ii) IgμU alleles (for PrP−/−IgμU−/− calves only) using the following primer pairs: BCμf; 5′-TGGTCACTCCAAGT GAGTCG-3′ (SEQ ID NO: 50) and BCElr; 5′-TGGAGTGAAATCAGGTGAA GG-3′ (SEQ ID NO: 51). The PCR reaction mixtures contained 17.9 μl water, 3 pt of 10× Ex Taq buffer, 4.8 μl of dNTP mixture, 10 μmol forward primer, 10 μmol of reverse primer, 2 μl of genomic DNA, and 0.3 μl of Ex Taq. Thirty cycles of PCR were performed by incubating the reaction mixtures at the following conditions: 85° C. for three minutes, 94° C. for one minute, 98° C. for 10 seconds, 60-62° C. for 30 seconds, and 72° C. for 1 minute. Following PCR, the reaction mixtures were analyzed by electrophoresis.
RT-PCR
From biopsy samples, total RNA was extracted using an RNeasy Mini kit (QIAGEN). One microliter of total RNA was subjected to first-strand cDNA synthesis (SuperScript First-Strand Synthesis System for RT-PCR, Invitrogen), followed by RT-PCR. The RT-PCR was carried out using the primer pair: 5′-AA GAAGCGACCAAAACCTGG-3′ (SEQ ID NO: 52) and 5′-GTAACGGTGCAT GTTTTCACG-3′ (SEQ ID NO: 53). The PCR reaction mixtures contained 32.5 pt water, 5 μl of 10× Ex Taq buffer (TAKARA), 8 μl of dNTP mixture, 10 μmol forward primer, 10 μmol of reverse primer, 2 μl of the first-strand cDNA, and 0.5 μl of Ex Taq (TAKARA). Thirty five cycles of PCR were performed by incubating the reaction mixtures at the following conditions: 85° C. for three minutes, 94° C. for one minute, 98° C. for 10 seconds, 62° C. for 30 seconds and 72° C. for 1 minute. For detection of bovine β-actin mRNA expression, primers bBAF (5′-ACATCCGCAAGGACCTCTAC-3′; SEQ ID NO: 54) and bBAR (5′-AACC GACTGCTGTCACCTTC-3′; SEQ ID NO: 55) were used under the same PCR conditions. To exclude the possibility of genomic DNA contamination, another set of RT-PCR reactions without reverse-transcriptase was also performed. Following PCR, the reaction mixtures were analyzed by electrophoresis. There were no positive PCR products in any biopsy sample prepared from a PrP−/− or PrP−/− IgμU−/− KO calf. This result verified the complete absence of prion gene expression in the calves.
Western Blotting
From biopsy or blood samples of live calves or brain samples of dead calves, total protein was extracted and the protein content was quantified using a Bio-Rad protein assay reagent. Western blot analysis was carried out by running approximately 75 μg of protein sample on a 12% SDS PAGE gel under non reducing conditions. The proteins were then transferred to a nitrocellulose membrane, and the membrane was stained using an anti-bovine prion protein monoclonal antibody (F 89/160.1.5; Alexis Biochemicals) as a primary antibody and secondarily stained with peroxidase-labeled affinity purified antibody directed to mouse IgG(H+L). The stained membrane was developed using an ECL plus western blotting detection system (Amersham Bioscience) and exposed to a Biomax light film by film developer. As a positive control, recombinant bovine PrP protein (Alexis Biochemicals) was used. As a negative control, protein extracts from murine fibroblasts were analyzed. As an internal positive control, the same blot was similarly stained with an anti-CDC2 monoclonal antibody. No prion protein band was detected in any biopsy sample prepared from a PrP−/− or PrP−/−IgμU−/−KO calf. This result verified the complete absence of prion protein expression in the calves.
Transfection of IgμU−/− PrP−/− cell line 8454 with pBCμAYKObsr vector was performed using the following standard electroporation protocol. The medium used to culture the bovine fetal fibroblasts contained 500 ml Alpha MEM (Gibco, 12561-049), 50 ml fetal calf serum (Hy-Clone #ABL13080), 5 ml penicillin-streptomycin (SIGMA), and 1 ml 2-mercaptoethanol (Gibco/BRL #21985-023). On the day prior to transfection, cells were seeded on a T175 tissue culture flask with a confluency of 80-100%, as determined by microscopic examination. On the day of transfection, about 107 bovine fibroblasts cells were trypsinized and washed once with alpha-MEM medium. After resuspension of the cells in 800 μl of alpha-MEM, 30 μg of the SrfI-digested KO vector (pBCμAYKObsr vector) dissolved in HEPES-buffered saline (HBS) containing 1 mM spermidine was added to the cell suspension and mixed well by pipetting. The cell-DNA suspension was transferred into an electroporation cuvette and electroporated at 550 V and 50 μF. After that, the electroporated cells were plated onto thirty 48-well plates with the alpha-MEM medium supplemented with the serum. After a 48 hour-culture, the medium was replaced with medium containing 10 μg/ml of blasticidine, and the cells were cultured for 2-3 weeks to select blasticidine resistant cells. After selection, all colonies which reached close to 100% confluency were divided into two replica plates (24-well and 48-well plates): one for genomic DNA extraction, and the other plate for nuclear transfer. Genomic DNA was extracted from the colonies to screen for the desired homologous recombination events by PCR.
As described above, the genomic DNA was independently extracted from each 24-well using the PUREGENE DNA isolation Kit (Gentra SYSTEMS) according to the manufacture's protocol. Each genomic DNA sample was resuspended in 20 μl of 10 mM Tris-Cl (pH8.0) and 1 mM EDTA (EDTA). Screening by PCR was performed using the following primer pair AYKObsrF2 (5′-GGTAGTGCAGTTTCGAATGGACAAAAGG-3′; SEQ ID NO: 56) and AYKObsrR2 (5′-TCAGGATTTGCAGCACACAGGAGTG-3′; SEQ ID NO: 57). The sequence of one primer is located in the KO vector, and the sequence of the other primer is located just outside of the integrated vector in the targeted endogenous locus. Therefore, the expected PCR product is detected only when the KO vector is integrated into the targeted locus by homologous recombination. The PCR reaction mixtures contained 17.9 μl water, 3 μl of 10× LA PCR buffer II (Mg2+ plus), 4.8 μl of dNTP mixture, 10 μmol of forward primer, 10 μmol of reverse primer, 2 μl of genomic DNA, and 0.3 μl of LA Taq. Forty cycles of PCR were performed by incubating the reaction mixtures under the following conditions: 85° C. for three minutes, 94° C. for one minute, 98° C. for 10 seconds, and 68° C. for 8 minutes. After PCR, the reaction mixtures were analyzed by electrophoresis. Out of 198 screened clones, 14 clones generated the expected PCR products. As a result of sequencing of the PCR products, the KO vector designed to target AY allele was exclusively integrated into the AY allele in all the clones.
The above knockout (IgμAY−/+IgμU−/−PrP−/−) cells or the nuclei from these cells were used in any of the nuclear transfer methods described herein to generate an IgμAY−/+IgμU−/−PrP−/− knockout ungulate (e.g., a knockout bovine calf). If desired, cells from a knockout fetus or live ungulate can be used as described below to generate homozygous prion knockout cells. In one particular method, fibroblasts (e.g., bovine primary fetal fibroblasts) were synchronized in mitosis with 1 μg/ml nocodazole for 18 hours, harvested by mitotic shake-off, and washed twice in phosphate buffered saline and once in cell lysis buffer (20 mM Hepes, pH 8.2, 5 mM MgCl2, 10 mM EDTA, 1 mM DTT and protease inhibitors). Sedimented cells were resuspended in one volume of ice-cold cell lysis buffer, allowed to swell on ice for one hour, and Dounce-homogenized using a tight-fitting glass pestle. The lysate was centrifuged at 15,000×g for 15 minutes at 4° C., and the supernatant (mitotic extract) was aliquoted, frozen in liquid nitrogen, and stored at −80° C. Fresh or frozen extracts were used. In vitro-matured oocytes were enucleated at 20 hpm. Transfected bovine fetal fibroblasts from selected colonies were washed in Ca2+/Mg2+-free Hank's Balanced Salt Solution (HBSS) and permeabilized by incubation of cells in suspension with 31.2 U Streptolysin 0 (SLO; Sigma) in 100 μl HBSS for 30 minutes in an approximately 37° C. water bath. Permeabilization was assessed by uptake of the membrane impermeant DNA stain, propidium iodide (0.1 μg/ml). Permeabilized fibroblasts were sedimented, washed, and incubated in 40 μl mitotic extract containing an ATP-generating system (1 mM ATP, 10 mM creatine phosphate, and 25 μg/ml creatine kinase) for 30-45 minutes at approximately 37° C. Aliquots were labeled with 0.1 μg/ml Hoechst 33342 to monitor chromatin condensation. At the end of incubation, the reaction mixture was diluted with 500 μl Alpha MEM/10% fetal bovine serum (Hyclone). Cells were fused to enucleated oocytes; oocytes were activated at 28 hpm, and embryos cultured to the blastocyst stage in vitro. Two embryos were transferred per recipient female. Pregnancies were monitored by ultrasonography, and C-sections were performed on recipients to recover fetuses for cell line production.
pBCμayKOhyg vector was transfected to the above IgμAY−/+IgμU−/−PrP−/− cell lines, except that the vector was digested with SalI before electroporation. As a result of screening of 119 hygromycin-resistant colonies, four clones were identified as being positive by PCR using primer pair ayKOhygF2 (5′-TGGTTGG CTTGTATGGAGCAGCAGAC-3′; SEQ ID NO: 58) and ayKOhygR2 (5′-TAGG ATATGCAGCACACAGGAGTGTGG-3′; SEQ ID NO: 59). Sequencing result of the PCR products demonstrated that the KO vector designed to target ay allele was exclusively integrated into the ay allele. From the above results, we conclude that both the AY and ay KO vectors can specifically target each allele in an allele-specific manner and produce correct targeted clones.
Using the methods described herein, we performed chromatin transfer from the identified IgμAY−/−IgμU−/−PrP−/− colonies and finally generated four IgμAY−/−IgμU−/−PrP−/− fetuses, from which we established four cell lines. Moreover, we obtained one IgμAY−/−IgμU−/−PrP−/− calf. Genotyping was performed as follows. The genomic DNA was extracted from fibroblasts originated from ear biopsy or from blood of the IgμAY−/−IgμU−/−PrP−/− triple homozygous KO calf using the PUREGENE DNA isolation Kit (Gentra SYSTEMS) according to the manufacture's protocol. Each genomic DNA sample was resuspended in 50-100 μl of 10 mM Tris-Cl (pH8.0) and 1 mM EDTA (EDTA). Confirmation by PCR was performed using the following primer pair 5′-TCTCTGGTGACGGCAATAGC-3′ (BCpf2; SEQ ID NO: 60) and 5′-CTTCGTGAGGAAGATGTCGG-3′ (BCtr2; SEQ ID NO: 61), which is compatible both with IgμAY and IgμU amplification, to confirm the absence of wild-type alleles of the IgμAY and IgμU genes, and another primer pair BPrPex3F (5′-CCACATAGGCAGTTGGATCC-3′ (SEQ ID NO: 629) and BPrPex3R (5′-ATAAGAGGCCTGCTCATGGC-3′ (SEQ ID NO: 63) to confirm the absence of wild-type alleles of the PrP gene. The PCR reaction mixtures contained 17.9 μl water, 3 μl of 10× Ex Taq buffer, 4.8 μl of dNTP mixture, 10 μmol forward primer, 10 μmol of reverse primer, 2 μl of genomic DNA, and 0.3 μl of Ex Taq. Thirty cycles of PCR were performed by incubating the reaction mixtures at the following conditions: 85° C. for three minutes, 94° C. for one minute, 98° C. for 10 seconds, 60-62° C. for 30 seconds and 72° C. for 1 min. After PCR, the reaction mixtures were analyzed by electrophoresis. The results demonstrated that the calf born was IgμAY−/−IgμU−/−PrP−/−.
From blood samples taken from the IgμAY−/−IgμU−/−PrP−/− triple homozygous KO calf, total protein was extracted and the protein content was quantified by using Bio-Rad protein assay reagent. Western blot analysis was carried out by running approximately 75 μg of protein sample on a 12% SDS page gel at non-reducing condition. The proteins were transferred to nitrocellulose membrane and the membrane was stained with anti-bovine Prion protein monoclonal antibody (F 89/160.1.5 from Alexis Biochemicals) as a primary antibody, followed by a peroxidase-labeled affinity purified secondary antibody to mouse IgG(H+L). The stained membrane was developed by ECL plus western blotting detection system (Amersham Bioscience) and exposed to Biomax light film by film developer. As a positive control, recombinant bovine PrP protein (Alexis Biochemicals) was used. As a negative control, protein extract from murine fibroblasts was used.
In the biopsy sample prepared from the IgμAY−/−IgμU−/−PrP−/− triple homozygous KO calf, no band was stained with antibody. This result verifies the complete absence of prion protein expression in the calf.
To confirm that the IgμAY−/−IgμU−/−PrP−/− triple homozygous KO calf lacked IgM protein, we performed flow cytometry analysis as described below. Peripheral blood was collected from the newborn calf by jugular venipunture into heparinized tubes. Whole white blood cells (leukocytes) were isolated from heparinized blood by red blood cell lysing using RBC-lysis buffer (Sigma, St. Louis, Mo.) and washing twice with sterile Hanks Buffered Salt Solution (HBSS) (Sigma, St. Louis, Mo.). Cells were resuspended in FACS staining medium (Phosphate Buffered Saline containing 4% horse serum or goat serum, 2 mM EDTA, and 0.2% sodium azide) and incubated for 30 minutes at room temperature for blocking non-specific binding. Sheep anti-bovine IgM-FITC (Bethyl Laboratories, Mongomery, Tex.) or donkey anti-sheep/bovine Ig-biotin antibody (Amersham Biosciences, Piscataway, N.J.) followed by Strepavidin-FITC or Sterpavidin-PE secondary antibody (Caltag Laboratories, Burlingame, Calif.) was used to label bovine surface IgM (sIgM) on the B cells. To label surface B220 marker on developing bovine B cells, mouse anti-bovine B220 (CD45R) antibody clone GS5A (VMRD, Pullman, Wash.) followed by anti-mouse IgG1-PE secondary antibody (Caltag Laboratories, Burlingame, Calif.) was used. Mouse anti-bovine CD21 Clone MCA1424 and mouse anti-ruminant CD43 Clone 1096 from Serotec Inc. (Raleigh, N.C.) antibodies followed by anti-mouse IgG1-PE secondary antibody (Caltag Laboratories, Burlingame, Calif.) were used to label surface CD21 and CD43 markers on bovine B cells. Fifty microliter cell suspension containing 106 cells (WBCs) in FACS staining medium were used for staining with each surface marker antibody alone as well as in combinations in V-bottom microtiter wells or tubes. Cells were incubated with primary antibodies at room temperature in dark for 20-30 minutes and washed twice with FACS wash buffer (Phosphate Buffered Saline containing 2 mM EDTA and 0.2% sodium azide) by centrifugation. Cells were resuspended again in 50 μl of FACS staining medium and incubated with appropriate fluorescent labeled secondary antibodies for 15-20 minutes at room temperature in dark. Finally cells were washed twice with FACS wash buffer and fixed with 2-4% formaldehyde in PBS. Surface labeled fixed cells were then analyzed and data acquired in FACScan flow cytometer (BD Biosciences, San Diego, Calif.). The list mode file data were finally analyzed using WinMDI software for single color or dual color profiles. The FACS result demonstrated complete absence of any developing B cell in the triple homozygous KO calf.
One use of animals having reduced IgM levels is the generation of xenogenous antibodies. One method of producing xenogenous antibody is to produce an animal having one or more human artificial chromosomes expressing antibody heavy chain and/or light chain. To this end, ΔΔHAC (λHAC) and κHAC were transferred from DT40 cell hybrids to Chinese hamster ovary (CHO) cells using microcell-mediated chromosome transfer (MMCT) (Kuroiwa et al. (2000) Nature Biotech. 18:1086-1090). The CHO clone containing λHAC (“ΔΔC10 clone”) was cultured in F12 (Gibco) medium supplemented with 10% FBS (Gibco), 1 mg/ml of G418, and 0.2 mg/ml of hygromycin B at 37° C. and 5% CO2. The ΔΔC10 clone was expanded into twelve T25 flasks. When the confluency reached 80-90%, colcemid (Sigma) was added to the medium at a final concentration of 0.1 μg/ml. After three days, the medium was exchanged with DMEM (Gibco) supplemented with 10 μg/ml of cytochalacin B (Sigma). The flasks were centrifuged for 60 minutes at 8,000 rpm to collect microcells. The microcells were purified through 8, 5, and 3-μm filters (Costar) and then resuspended in DMEM medium. The microcells were used for fusion with bovine fibroblasts as described below.
Bovine fetal fibroblasts were cultured in A-MEM (Gibco) medium supplemented with 10% FBS (Gibco) at 37° C. and 5% CO2. The fibroblasts were expanded in a T175 flask. When the confluency reached 70-80%, the cells were detached from the flask with 0.05% trypsin. The fibroblast cells were washed twice with DMEM medium and then overlayed on the microcell suspension. After the microcell-fibroblast suspension was centrifuged for five minutes at 1,500 rpm, PEG1500 (Roche) was added to the pellet according to the manufacturer's protocol to enable fusion of the microcells with the bovine fibroblasts. After fusion, the fused cells were plated into six 24-well plates and cultured in α-MEM medium supplemented with 10% FBS for 24 hours. The medium was then exchanged with medium containing 0.8 mg/ml of G418. After growth in the presence of the G418 antibiotic for about two weeks, the G418 resistant, fused cells were selected. These G418-resistant clones were used for chromatin transfer, as described herein.
To examine whether HAC/IgμAY−/−IgμU−/− fetuses could express human immunoglobulin such as IgM, IgG, Igλ and Igκ, we collected HAC/IgμAY−/−/IgμU−/− fetuses at 180 days of gestation. Total RNA was extracted from the spleen by using RNeasy Mini kit (QIAGEN). One microliter of total RNA was subjected to first-strand cDNA synthesis (SuperScript First-Strand Synthesis System for RT-PCR, Invitrogen), followed by RT-PCR. To detect human IgM expression, RT-PCR reaction was carried out using primer pair; 5′-AGGCCAGCATCTGCGAG GAT-3′ (CH3—F3; SEQ ID NO: 64) and 5′-GTGGCAGCAAGTAGACATCG-3′ (CH4-R2; SEQ ID NO: 65). For human IgG expression, the following primers were used: 5′-CAGGTGCAGCTGGTGCAGTCTGG-3′ (SEQ ID NO: 66, 5′-CAGGTCACCTTGAAGGAGTCTGG-3′ (SEQ ID NO: 67), 5′-GAGGTGCA GCTGGTGGAGTCTGG-3′ (SEQ ID NO: 68), 5′-CAGGTGCAGCTGCAGGAG TCGGG-3′ (SEQ ID NO: 69), 5′-GAGGTGCAGCTGGTGCAGTCTGG-3′, 5′-CAGGTACAGCTGCAGCAGTCAGG-3′ (SEQ ID NO: 70), and 5′-CAGGTGCA GCTGGTGCAGTCTGG-3′ (SEQ ID NO: 71) (VH All Mix) in combination with 5′-CACCACGCTGCTGAGGGAGTAGAGT-3′ (hCg1R2; SEQ ID NO: 72). For human Igλ expression, the following primer pair was used: 5′-TCCTCTGAGGAGCTTCAAGC-3′ (hCL-F2; SEQ ID NO: 73) and 5′-AGGGTTTATTGAGT GCAGGG-3′ (hCL-R2; SEQ ID NO: 74). The PCR reaction mixtures contained 32.5 μl water, 5 μt of 10× Ex Taq buffer (TAKARA), 8 μl of dNTP mixture, 10 μmol forward primer, 10 μmol of reverse primer, 2 μl of the first-strand cDNA, and 0.5 μl of Ex Taq (TAKARA). Thirty five cycles of PCR were performed by incubating the reaction mixtures at the following conditions: 85° C. for three minutes, 94° C. for one minute, 98° C. for 10 seconds, 60-62° C. for 30 seconds and 72° C. for 1 minute. After PCR, the reaction mixtures were analyzed by electrophoresis. From a λHAC/IgμAY−/−/IgμU−/− fetus, human IgM, IgG, and Igλ expression was detected by RT-PCR.
To confirm cell surface expression of human immunoglobulin at the protein level, we also performed flow cytometry analysis as described above. Goat anti-human IgM-FITC (Bethyl Laboratories, Mongomery, Tex.), goat anti-human IgM-FITC (Serotec Inc., Raleigh, N.C.), or goat anti-human IgM-PE (Serotec Inc., Raleigh, N.C.) antibody was used to label the human sIgM expressed on the peripheral blood B cells of ≧180 day HAC fetuses. To detect the light chains expressed on the B cells, goat anti-human lambda-FITC antibody (Bethyl Laboratories) or goat anti-human lambda-PE antibody (Serotec Inc) was used. For dual color analysis, a FITC-labeled anti-human IgM antibody and PE-labeled light chain antibody combination was used. We detected cell populations that were positive with the anti-human IgM and light chain antibodies. Furthermore, we performed sandwich ELISA analysis to detect secreted human IgG in λHAC/IgμAY−/−/IgμU−/− calves using an affinity purified capture antibody and an appropriate HRP-enzyme labeled detection antibody. Details of the capture antibody and detection antibody for each assay are given in Table 6 below.
The capture antibody diluted in coating buffer (0.05 M sodium carbonate, pH 9.6) was coated on the microtiter plates (Nunc ImmunoMaxiSorp Elisa plates) by incubating at room temperature for 1.5 hours. After coating the capture antibody, the plates were washed with phosphate buffered saline (PBS)/Tween 20 buffer 3-5 times using an automated plate washer. Appropriate standards (Calibrators) were added in serial dilutions for quantification using a standard curve. Positive controls and negative controls were included in all assays for QC check. Serum samples from λHAC/IgμAY−/−/IgμU−/− calves were added then into duplicate wells in four serial dilutions and incubated for 1 hour at room temperature. After serum immunoglubulins were captured, the plates are washed again with PBS-Tween buffer 3-5 times using automated plate washer. HRP-enzyme labeled appropriate detection antibody was added into all the wells and incubated for 1 hour at room temperature. At the end of incubation, the plates were washed again with PBS/Tween buffer 3-5 times using automated plate washer. The bound antibodies were detected by adding TMB-Substrate solution (KPL Inc, Gaithersburg, Mass.) and incubating for 10-20 minutes at room temperature. The reaction was stopped by addition of 10% phosphoric acid. The plates were then read on a microtiter plate reader using KC4 software. Data were analyzed by KC4 software and values were determined by interpolation on a four-parameter standard curve. In blood sample collected at 14 days after birth, 7.1 μg/ml of human IgG was detected by ELISA.
All publications and patents cited in this specification are incorporated herein by reference as if each individual publication or patent were specifically and individually indicated to be incorporated by reference. Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it will be readily apparent to those of ordinary skill in the art in light of the teachings of this invention that certain changes and modifications may be made thereto without departing from the spirit or scope of the appended claims.
This application claims benefit from U.S. Provisional Application Ser. Nos. 60/654,445 (filed Apr. 22, 2004), 60/654,458 (filed Apr. 22, 2004), and 60/657,186 (filed Feb. 28, 2005), each of which is hereby incorporated by reference.
| Number | Date | Country | |
|---|---|---|---|
| 60654445 | Feb 2005 | US | |
| 60564458 | Apr 2004 | US | |
| 60657186 | Feb 2005 | US |
