Claims
- 1. An assay method for detecting anti-Treponema pallidum antibodies in a sample, comprising:reacting with said sample a Treponema pallidum fused antigen, wherein the fused antigen consists of a plurality of surface antigens of Treponema pallidum, wherein said surface antigens include at least one antigen selected from the group consisting of 15-kilodalton surface antigen of Treponema pallidum, 17-kilodalton surface antigen of Treponema pallidum, and 47-kilodalton surface antigen of Treponema pallidum; and detecting the reaction of said fused antigen with said antibodies.
- 2. The method of claim 1, wherein the fused antigen consists of two amino acid sequences, each constituting a surface antigen of Treponema pallidum selected from the group consisting of the 15-kilodalton surface antigen and the 17-kilodalton surface antigen,wherein the 15-kilodalton antigen is amino terminal to the 17-kilodalton antigen, or the 17-kilodalton antigen is amino terminal to the 15-kilodalton antigen.
- 3. The method of claim 1, wherein the fused antigen consists of two amino acid sequences, each constituting a surface antigen of Treponema pallidum selected from the group consisting of the 15-kilodalton and the 47-kilodalton antigen,wherein a 15-kilodalton antigen is amino terminal to the 47-kilodalton antigen, or the 47-kilodalton antigen is amino terminal to the 15-kilodalton antigen.
- 4. The method of claim 1, wherein the fused antigen consists of two amino acid sequences, each constituting a surface antigen of Treponema pallidum selected from the group consisting of the 17-kilodalton and the 47-kilodalton antigen,wherein a 17-kilodalton antigen is amino terminal to the 47-kilodalton antigen, or the 47-kilodalton antigen is amino terminal to the 17-kilodalton antigen.
- 5. The method of claim 1, wherein the fused antigen consists of two amino acid sequences, each constituting the 15-kilodalton antigen.
- 6. The method of claim 1, wherein the fused antigen consists of three amino acid sequences which are 15-kilodalton surface antigen of Treponema pallidum, 17-kilodalton surface antigen of Treponema pallidum, and 47-kilodalton surface antigen of Treponema pallidum.
- 7. The method of claim 1, wherein the fused antigen consists of four amino acid sequences which may be the same or different, each constituting a surface antigen of Treponema pallidum selected from the group consisting of the 15-kilodalton antigen, the 17-kilodalton antigen, and the 47-kilodalton antigen.
- 8. The method of claim 1, wherein the fused antigen includes at least two surface antigens selected from the group consisting of 15-kilodalton surface antigen of Treponema pallidum, and 47-kilodalton surface antigen of Treponema pallidum, said at least two surface antigens of Treponema pallidum being the same or different.
- 9. The method of claim 1, wherein said fused antigen is bound to a carrier.
- 10. The method of claim 9, wherein said carrier is selected from the group consisting of latex particles, gelatin particles, and magnetic particles.
- 11. The method of claim 1, further comprising measuring the amount of said antibodies in said sample.
- 12. The method of claim 1, wherein said detecting is accomplished by agglutination.
- 13. The method of claim 1, wherein the reaction of said fused antigen with said antibodies is detected with an antihuman immunoglobin labeled with an enzyme.
- 14. The method of claim 13, wherein said enzyme is peroxidase.
Priority Claims (1)
Number |
Date |
Country |
Kind |
7-350072 |
Dec 1995 |
JP |
|
Parent Case Info
This application is a divisional of U.S. application Ser. No. 08/773,106, filed on Dec. 26, 1996, now U.S. Pat. No. 6,248,331.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4976958 |
Shinnick et al. |
Dec 1990 |
A |
Foreign Referenced Citations (3)
Number |
Date |
Country |
0670494 |
Sep 1995 |
EP |
9504145 |
Feb 1995 |
WO |
9512676 |
May 1995 |
WO |
Non-Patent Literature Citations (1)
Entry |
Weigel et al (Infection and Immunity, 60(4):1568-76, 4/92). |