Patent Application
20110237644
The present invention relates to a use of a polypeptide selected from the group consisting of USP13, USP26, USP38, USP42 or USP46 as a screening tool for an agent for treating cancer.
Myeloid cell leukemia-1 (Mcl-1), originally identified in differentiating myeloid cells, belongs to the anti-apoptotic Bcl-2 family members (Bcl-2, Bcl-XL, Bcl-W, A1). Mcl-1 acts at an early step in apoptosis induced by various death stimuli, particularly DNA damages, and serves as a major survival factor. More precisely, Mcl-1, which promotes cell survival by interfering in a cascade of events leading to release of cytochrome c from mitochondria, is a short half-life protein. Processes that regulate Mcl-1 are thus critically important in pathological situations such as cancer.
The degradation of Mcl-1 can be blocked by proteasome inhibitors suggesting a role for the Ubiquitin-Proteasome pathway in apoptosis.
The ubiquitin-mediated degradation is a tightly regulated process where proteins are tagged with ubiquitin moeties through a series of enzymatic reactions involving an E1-activating enzyme, an E2-conjugating enzyme, and an E3 ubiquitin ligase that determines substrate specificity. Proteins are then degraded by the proteasome. The reverse process also occurs and leads to the removal of the ubiquitin chain, i.e. deubiquitination. This latter reaction is performed, in part, by enzymes termed ubiquitin specific proteases (USPs) and results in restoration of substrate to its normal state.
The ubiquitin specific proteases (USPs) belong to the broad family of de-ubiquitinating enzymes (DUBs). The human genome encodes approximately 95 putative deubiquitinating enzymes (DUBs) which belong to the super-family of proteases. These enzymes are divided into 5 sub-families, among which the most-known sub-classes are USP (Ubiquitin Specific Proteases) and UCH (Ubiquitin C-terminal hydrolases). The 60 human USP proteins are cysteine proteases and their catalytic domain contain a ‘Cysteine box’ and an ‘Histidine Box’. The main function of USP is to remove ubiquitin from specific protein substrates.
Clinical success with proteasome inhibitors strengthens the notion that modulating other steps in Ubiquitin-Proteasome pathways, such as E3s or USP, might be therapeutically successful with more specificity and efficacy.
It has been also reported in a publication the identification of an E3 ligase named Mule/ARF-BP1/UreB1 that is required and sufficient for the poly-ubiquitination of Mcl-1. Interestingly, down-regulation of Mule expression resulted in stabilization and accumulation of Mcl-1, leading to cells becoming more resistant to killing by genotoxic agents.
The results disclosed in the present invention indicate that USPs specific to Mcl-1 exist and would therefore act on the stabilization of cellular Mcl-1 to control apoptosis. Inhibition of these USPs would lead to an increase of Mcl-1 degradation by the proteasome promoting the onset of apoptosis. This observation offers a new way to target Mcl-1 by inhibiting the Mcl-1-regulating USP protein with small molecule compounds.
A first object of the invention is a use of a polypeptide selected from the group consisting of
The Mcl-1-regulating USPs disclosed in the invention are USP13 (GI: 4507848), USP26 (GI: 13994267), USP38 (GI: 31559774), USP42 (GI: 79750943), USP46 (GI: 31377708) and can cleave either peptide bonds linking ubiquitin as part of a precursor fusion protein, releasing free ubiquitin moieties or cleave bonds conjugating ubiquitin (post-translationally) to proteins.
In a particular embodiment, the invention provides the use of USP46, a functional fragment or a substantially homologous sequence thereof as a screening tool for an agent for treating cancer.
USP46 is a small 366 AA-long protein of unknown function which contains the UCH domain, a catalytic domain involved in deubiquitinating functions. USP46 was found to be widely distributed in human tissues and to be active in vitro. Recently, an amplicon on chromosome 4q12, containing USP46 as flanking gene, has been described in glioblastomas. USP46 gene amplification was detected in several glioblastoma and glioblastoma cell cultures. USP46 sequence analysis identified a human paralog, USP12=UBH1 (NP—872294), which exhibits 88% identity in protein sequence and 76% identity in nucleotide sequence. Furthermore, the catalytic domain of USP46 and USP12 exhibit 100% identity in protein sequence. Biochemical assay revealed that the mouse UBH1, with 98.3% amino acid identity with human UBH1, exhibits deubiquitinating enzyme activity.
A “functional fragment” retains one or more of the biological activities of the protein such as the ability to hydrolyze peptide bonds with ubiquitin or comprises a domain or motif e.g. catalytic site, USP signature, domain including the cysteine or histidine ubiquitin recognition sites, ubiquitin binding sites, or sites important for carrying out the other functions of the protease.
The terms “homology” and “substantially homologous” when used herein with respect to a sequence mean that a sequence when compared to its corresponding reference sequence, has substantially the same structure and function. When a position in the reference sequence is occupied by the same amino acid the molecules are homologous at that position (i.e. there is identity at that position). The percentage of homology between the substantially homologous sequence and reference sequence desirably is at least 85%, more desirably at least 88%, preferably at least 95%, still more preferably at least 99%.
In a second aspect of the invention, a use of a cell which is transformed with an expression vector comprising a polynucleotide encoding a polypeptide consisting of USP13, USP26, USP38, USP42 or USP46, a functional fragment or a substantially homologous sequence thereof is provided. The cell is expressing the polypeptide as a screening tool for an agent for treating cancer.
The expression “screening tool” as used herein means a tool used for screening more particularly a polypeptide or a cell expressing a polypeptide used for screening.
As used herein, the expression “agent for treating cancer” includes not only a drug for curing a patient suffering from cancer, but also a drug for preventing a progress of cancer. “Treating cancer” refers to the prophylactic or preferably therapeutic (including but not limited to palliative, curative, symptom-alleviating, symptom-reducing, disease- or symptom-suppressing, progression-delaying) treatment of cancer.
The term “cancer” means preferably a variety of proliferative diseases, including those mentioned below but are not limited to solid tumors (including benign or especially malign types) such as sarcoma (e.g. Ewing sarcoma, Kaposi's sarcoma or soft part sarcomas such as Dermatofibrosarcoma protuberans), gastrointestinal stromal tumors (GIST), seminoma, carcinoids, mast cell tumors, lung carcinomas, such as small or large cell lung carcinoma, bronchial carcinomas, such as small cell bronchial carcinoma, seminomas, dysgerminomas, testicular intraepithelial neoplasias, melanomas, mammary carcinomas, neuroblastomas, papillary/follicular thyroid carcinoma, malign lymphomas, Non Hodgkin's lymphoma, multiple endocrine neoplasia type 2 (MEN 2), pheochromocytoma, thyroid carcinoma, e.g. medullary thyroid carcinoma, parathyroid hyperplasia/adenoma, mammary carcinoma, colon cancer, colorectal adenoma, ovarian cancer, prostate carcinoma, gliobastoma, brain tumors, prostate carcinoma (also including adenocarcinomas and bone metastasis), malignant gliomas (anaplastic astrocytomas/glioblastomas), pancreatic cancer, malignant pleural mesothelioma, haemangioblastoma, haemangioma, carcinoma of the kidney, liver, adrenal gland, bladder, stomach (especially gastric tumors), rectum, vagina, cervix, endometrium, multiple myeloma, tumors of the head and neck, e.g. squamous carcinoma of the head and neck, including neoplasias, especially of epithelial character, e.g. in the case of mammary carcinoma, malignant nephrosclerosis; or further of other hyperplasias or proliferative diseases.
A “cell which is transformed” as used herein, refers to a prokaryotic or eukaryotic cell that contains heterologous DNA that has been introduced into the cell by any means, e.g. electroporation, calcium phosphate precipitation, microinjection, transformation, viral infection, and the like.
A “vector” is a nucleic acid molecule into which heterologous nucleic acid may be inserted which can then be introduced into an appropriate host cell. Vectors preferably have one or more origin replication and one or more site into which the recombinant DNA can be inserted. Common vectors include plasmids, viral genomes and artificial chromosomes.
A third aspect of the invention encompasses a method for screening an agent for treating cancer which requires bringing a cell which is transformed with an expression vector comprising a polynucleotide encoding a polypeptide consisting of USP13, USP26, USP38, USP42 or USP46, a functional fragment or a substantially homologous sequence thereof or a cell membrane thereof into contact with a compound to be tested and analyzing whether or not Mcl-1 is stabilized.
In a one preferred aspect, in the screening method of the present invention, the agent for treating cancer is an agent destabilizing Mcl-1. In another aspect, the agent for treating cancer is an inhibitor of the catalytic activity of USPs of the invention. Preferably, in the screening method of the present invention, the agent for treating cancer is an inhibitor of a polypeptide consisting of USP13, USP26, USP38, USP42 or USP46, a functional fragment or a substantially homologous sequence thereof.
As used herein, “stabilization” and “destabilization” is related to the half-life assay of Mcl-1.
Another preferred aspect provides a method for screening an agent for treating cancer which requires bringing a cell which is transformed with an expression vector comprising a polynucleotide encoding a polypeptide consisting of USP13, USP26, USP38, USP42 or USP46, a functional fragment or a substantially homologous sequence thereof or a cell membrane thereof into contact with a compound to be tested and analyzing the rate of ubiquitination.
Preferably, the agent for treating cancer is an inhibitor of USPs inducing the increase of ubiquitinated substrate, i.e. ubiquitinated-Mcl-1.
Cell-based systems in drug screening assays can be native or involving recombinant host cells expressing the ubiquitin specific proteases.
The identified agent modulating ubiquitin specific proteases can increase or decrease affinity for ubiquitinated substrate, increase or decrease the rate of binding to a known binding molecule to USP, compete or displace a known binding molecule to the ubiquitin protease.
Yet another aspect, the invention is directed to a method for the treatment of cancer comprising administering to a patient in need thereof an effective amount of an agent decreasing the activity of a polypeptide USP13, USP26, USP38, USP42 or USP46 of the invention.
Another aspect is directed to a method for the treatment of cancer comprising administering to a patient in need thereof an effective amount of an agent decreasing the activity of a polypeptide disclosed in the invention, e.g. USP13, USP26, USP38, USP42 or USP46 and an agent active against cancer.
An “agent decreasing the activity”, as utilized herein, may refer to compound having the ability to inhibit the catalytic/deubiquitinating activity of the ubiquitin specific protease or the interaction between the ubiquitin specific protease and a target molecule that normally interacts with the ubiquitin specific protease. The target can be ubiquitin or ubiquitinated substrate.
As used herein the expression “agent active against cancer” relates to antiproliferative agents including, but are not limited to aromatase inhibitors; antiestrogens; topoisomerase I inhibitors; topoisomerase II inhibitors; microtubule active agents; alkylating agents; histone deacetylase inhibitors; compounds which induce cell differentiation processes; cyclooxygenase inhibitors; MMP inhibitors; mTOR inhibitors; antineoplastic antimetabolites, platin compounds; compounds targeting/decreasing a protein or lipid kinase activity and further anti-angiogenic compounds; compounds which target, decrease or inhibit the activity of a protein or lipid phosphatase; gonadorelin agonists; anti-androgens; methionine aminopeptidase inhibitors; bisphosphonates; biological response modifiers; antiproliferative antibodies; heparanase inhibitors; inhibitors of Ras oncogenic isoforms; telomerase inhibitors; proteasome inhibitors; agents used in the treatment of hematologic malignancies; compounds which target, decrease or inhibit the activity of Flt-3; Hsp90 inhibitors; and temozolomide.
In a particular embodiment, the agent active against cancer is ABT737 which has a low affinity for Mcl-1 and A1, members of the anti-apoptotic Bcl-2 family, and may exhibit limited cytotoxic effects in cells with high endogenous levels of Mcl-1. Ultimately, inhibiting USP46 with small molecule compounds could facilitate Mcl-1 targeting and confer sensitivity to Bcl-2/Bcl-XL inhibitors.
The invention further identified an ABT-737 sensitization following USP46 silencing in different cell lines such as colon, cervical, prostate and lung cancer cell lines corresponding respectively to HCT116, HeLa, PC-3, H196 and H1703 cell lines.
Yet another aspect, the invention is directed to a method for delaying the progression of cancer comprising administering to a patient in need thereof an effective amount of an agent decreasing the activity of a polypeptide disclosed in the invention, e.g. USP13, USP26, USP38, USP42 or USP46.
Western blot (anti-Mcl-1) revealing expression of endogenous Mcl-1 in the presence of various siRNA targeting either Luc (luciferase, negative control), Mcl-1, (positive control) or USP13 (siRNA13v1, siRNA13v2, siRNA13v3) in HCT116 cells. A weaker exposition is also presented in B. Anti-stat3 antibody was used as loading control. The time course corresponds to the time for which cells were exposed to the protein synthesis inhibitor (cycloheximide) before analysis.
Western blot (anti-Mcl-1) revealing expression of endogenous Mcl-1 in the presence of various siRNA targeting either Luc (luciferase, negative control), Mcl-1, (positive control) or USP26 (siRNA26v1, siRNA26v2, siRNA26v3) in HCT116 cells. Anti-stat3 antibody was used as loading control. The time course corresponds to the time for which cells were exposed to the protein synthesis inhibitor (cycloheximide) before analysis.
Western blot (anti-Mcl-1) revealing expression of endogenous Mcl-1 in the presence of various siRNA targeting either Luc (luciferase, negative control), Mcl-1, (positive control) or USP38 (siRNA38v1, siRNA38v2, siRNA38v3) in HCT116 cells. Anti-stat3 antibody was used as loading control. The time course corresponds to the time for which cells were exposed to the protein synthesis inhibitor (cycloheximide) before analysis.
Western blot (anti-Mcl-1) revealing expression of endogenous Mcl-1 in the presence of various siRNA targeting either Luc (luciferase, negative control), Mcl-1, (positive control) or USP42 (siRNA42v1, siRNA42v2, siRNA42v3) in HCT116 cells. Anti-stat3 antibody was used as loading control. The time course corresponds to the time for which cells were exposed to the protein synthesis inhibitor (cycloheximide) before analysis.
Western blot (anti-Mcl-1) revealing expression of endogenous Mcl-1 in the presence of various siRNA targeting either Luc (luciferase, negative control), Mcl-1, (positive control) or USP46 (siRNA46v1, siRNA46v2, siRNA46v3) in HCT116 cells. Anti-stat3 antibody was used as loading control. The time course corresponds to the time for which cells were exposed to the protein synthesis inhibitor (cycloheximide) before analysis.
Mcl-1 endogenous level was determines in PC3 cells in the presence of several siRNA targeting either luciferase, Mcl-1 or USP46 (A). anti-actin antibody was used as loading control. Mcl-1 (B) and USP46 (C) mRNA levels were determined by quantitative RT-PCR following PC3 transfection with siRNA targeting either luciferase (Luc, negative control), Mcl-1 or USP46. The results are shown as means+/−SD of quadruplicate values.
Mcl-1 endogenous level was determines in H196 cells in the presence of several siRNA targeting either luciferase, Mcl-1 or USP46 (A). anti-actin antibody was used as loading control. Mcl-1 (B) and USP46 (C) mRNA levels were determined by quantitative RT-PCR following PC3 transfection with siRNA targeting either luciferase (Luc, negative control), Mcl-1 or USP46. The results are shown as means+/−SD of quadruplicate values.
Mcl-1 endogenous level was determines in H1703 cells in the presence of several siRNA targeting either luciferase, Mcl-1 or USP46 (A). anti-actin antibody was used as loading control. Mcl-1 (B) and USP46 (C) mRNA levels were determined by quantitative RT-PCR following PC3 transfection with siRNA targeting either luciferase (Luc, negative control), Mcl-1 or USP46. The results are shown as means+/−SD of quadruplicate values.
Mcl-1 endogenous level was determines in HEK293 cells in the presence of several siRNA targeting either luciferase, Mcl-1 or USP46 (A). anti-actin antibody was used as loading control. Mcl-1 (B) and USP46 (C) mRNA levels were determined by quantitative RT-PCR following PC3 transfection with siRNA targeting either luciferase (Luc, negative control), Mcl-1 or USP46. The results are shown as means+/−SD of quadruplicate values.
Mcl-1 endogenous level was determines in H196 cells in the presence of several siRNA targeting either luciferase, Mcl-1 or USP46 (A). anti-actin antibody was used as loading control. Mcl-1 (B) and USP46 (C) mRNA levels were determined by quantitative RT-PCR following PC3 transfection with siRNA targeting either luciferase (Luc, negative control), Mcl-1 or USP46. The results are shown as means+/−SD of quadruplicate values.
1.1 siRNA Design and Synthesis
19-mer siRNA synthesized chemically using phosphoramidites were purchased from Proligo. siRNAs were designed as described by Tuschl and colleagues (Elbashir et al. (2001). Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411, 494-8) with particular attention to thermodynamic characteristics (Khvorova et al. (2003). Functional siRNAs and miRNAs exhibit strand bias. Cell 115, 209-16). A tool dedicated to study siRNA specificity was developed in-house. Briefly, NCBI NR and EST databases were requested with each siRNA and target gene by BLAST algorithm. siRNA matching exactly (19/19) or partially (18/19 nucleotides) with an irrelevant NR or EST entry were discarded.
Human colon carcinoma HCT116 cells were maintained in McCoy's 5A medium containing 10% FBS. The culture media was supplemented with 100 units/ml penicillin and 0.1 mg/ml streptomycin, and cell lines were incubated at 37° C. in a humidified atmosphere containing 5% CO2.
For the screening, the indicated amounts of siRNA (40 pmol, 72 nM final) were complexed with 2 μl of Oligofectamine (Invitrogen) in Opti-MEM (Invitrogen) and added to 24-well plates prior to cell seeding. 150 000 HCT116 cells were next plated. Opti-MEM was replaced with appropriate medium 5 hours after transfection. After 72 h of transfection and before harvesting, cells were treated or not with cycloheximide (30 μg/ml) for 30 min or 1 hour or treated with MG132 (30 μM) for 90 min.
Cells were washed once in chilled PBS and harvested by trypsination. Floating and trypsinized cells were pooled and collected by centrifugation. Cell pellets were resuspended in lysis buffer (2% SDS containing 1× protease inhibitor cocktail, Sigma), placed at 100° C. for 10 minutes and sonicated for 5 seconds. Proteins were quantitated using bicinchoninic acid (Sigma-Aldrich) according to manufacturer's instructions. Lysates were resolved by SDS-polyacrylamide (10%) gel electrophoresis (SDS-PAGE), transferred to a nitrocellulose membrane and probed with Mcl-1 rabbit polyclonal antibody (sc-819; SantaCruz), Stat3 rabbit polyclonal antibody (9132; Cell Signaling), Actin mouse monoclonal antibody (A4700; Sigma), or GAPDH mouse monoclonal antibody (Ab8245; AbCam). HRP-conjugated anti-rabbit (7074; Cell Signaling) and HRP conjugated anti-mouse (115-035-003; Jackson ImmunoResearch) antibodies were used as secondary antibodies. Signal was detected using ECL reagent (Amersham) following manufacturer's instructions.
All cell lines used were maintained in their respective medium. All culture media were supplemented with 100 units/ml penicillin and 0.1 mg/ml streptomycin, and cell lines were incubated at 37° C. in a humidified atmosphere containing 5% CO2. For siRNA transfection, siRNA (40 pmol, 72 nM final) were complexed with 2 μl of Oligofectamine (Invitrogen) in Opti-MEM (Invitrogen) and added to 24-well plates prior to cell seeding. In all conditions, Opti-MEM was replaced with appropriate medium 5 hours after transfection. When Hiperfect was used (Qiagen), siRNA (40 pmol, 72 nM final) were complexed with 3 μl of Hiperfect (Qiagen) in Opti-MEM (Invitrogen) and added to 24-well plates after cell seeding. After 48 h or 72 h of transfection and before harvesting, cells were treated or not with ABT737 for the indicated time points and at the concentration indicated.
Difficult-to-transfect cells such as H1703 cells were transfected using the AMAXA procedure. Cells are first suspended in special cuvette in the presence of siRNA of interest. An electric pulse, defined according to the cell line used, was next delivered in the Nucleofector device. Nucleofected cell were next plated in 24 or 6-wells plates according to manufacturer's instructions and were treated with ABT737 as described above.
Deubiquitinating enzyme activity was monitored in a fluorometric assay, using ubiquitin-AMC or Ub-AMC (ubiquitin-7-amido-4-methylcoumarin, Boston Biochem, U-550) as the substrate, as previously described (Dang et al. (1998) Kinetic and mechanistic studies on the hydrolysis of ubiquitin C-terminal 7-amido-4-methylcoumarin by deubiquitinating enzymes. Biochemistry. 37(7):1868-79). The AMC released from the C-terminus of ubiquitin displays enhanced fluorescence, which can be monitored with a fluorescence reader. Both enzyme and substrate were freshly prepared in USP46 reaction buffer (50 mM Tris-HCl [pH 8.4], 0.5 mM EDTA, 0.05 mg/ml BSA, 5 mM DTT) for each run. The reaction mixture between USP46 and Ub-AMC was incubated at room temperature for 2 h and reaction was stopped by adding 10 μl of acetic acid (250 mM final concentration). Fluorescence emission intensity was measured on a PHERAstar (BMG Labtech) machine, using a coumarin filter set (λex=360 nm, λem=460 nm).
To identify the Ubiquitin Specific Proteases involved in Mcl-1 deubiquitination and thus Mcl-1 stabilization, the entire USP family was screened by RNA interference on Mcl-1 half-life in HCT116 cancer cells. Out of the 60 USP proteins screened, 10 USPs (USP5, USP13, USP19, USP21, USP22, USP26, USP28, USP38, USP42 and USP46) has been identified with the expected phenotype, i.e. a substantial decrease of Mcl-1 (at least 2 out of 3 siRNA exhibiting more than 50% Mcl-1 decrease at one time point). Schematic representation of Mcl-1 modulation following USP silencing and cycloheximide treatment are presented in
To confirm the results obtained in the primary screen, an independent experiment was done with the 10 selected USPs. The data have been confirmed for 5 USPs (USP13, USP26, USP38, USP42 and USP46) out of 10 USPs of the primary screen results. Western blot results of the confirmation experiments are shown in
Therefore, from the screening of the 60 human USPs by RNA interference for Mcl-1 half-life in HCT116 cells, the involvement of 5 USPs (USP13, USP26, USP38, USP42 and USP46) in Mcl-1 stabilization has been confirmed. All these proteins contain amino acids essential for catalytic activity, thus suggesting a functional deubiquitinating activity. Indeed, USP42, USP46 and USP38 have been previously shown to be active in vitro.
2.3 Effect of USP46 Silencing on Mcl-1 mRNA and Protein Levels in PC3, H196, H1703, HEK293 and HeLa Cells
The effect of Mcl-1 siRNA on endogenous Mcl-1 protein level in PC3, H196, H1703, HEK293 and HeLa cells is monitored and showed a strong decrease of Mcl-1 level, indicating very good transfection efficiency in these cell lines (respectively
Endogenous Mcl-1 mRNA levels were next determined by quantitative PCR in PC3, H196, H1703, HEK293 and HeLa cells to exclude a transcriptional regulation of Mcl-1 level by USP46. No significant decrease of Mcl-1 mRNA levels following USP46 silencing was observed (respectively
>68% of decrease in PC3 cells
>62% of decrease in H196 cells
>46% of decrease in H1703 cells
>90% of decrease in HEK293 cells
>80% of decrease in HeLa cells are confirmed (respectively
These findings show that USP46 is involved in Mcl-1 stabilization in PC3, H196, H1703, HEK293 and HeLa cell lines and thus confirm the previous findings in HCT116 cells.
This assay was used to evaluate the effect of USP46 siRNA on the sensitization of HeLa cells to ABT-737-mediated apoptosis. Our positive control, Mcl-1 siRNA, sensitized cells to ABT-737-mediated apoptosis as shown by a 2.7-fold increase of caspase 3 activity (
To confirm the effect of USP46 siRNA on the sensitization of cells to ABT-737-induced apoptosis in HeLa cells, the number of subG1-phase cells is quantified which reflect mainly apoptosis. Similarly to Mcl-1 siRNA, USP46 siRNAs induced apoptosis in non-treated HeLa cancer cells (
In conclusion, similarly to Mcl-1 silencing, USP46 silencing decreases Mcl-1 level, induces apoptosis in non-treated HeLa cancer cells and sensitizes HeLa cells to ABT-737-induced cell death. Interestingly, these differences in ABT-737 sensitization always correlated with the silencing efficiency on Mcl-1 level: the more Mcl-1 is decreased following USP46 siRNA transfection, the more USP46-mediated sensitization is observed. USP46 silencing was shown to significantly sensitize cells to ABT-737 at an intermediate level between the negative control (Luc siRNA) and the positive control (Mcl-1 silencing), as evaluated by caspase 3 assay, PARP cleavage and subG1 analysis. Taken together, these results confirm the involvement of USP46 on Mcl-1 regulation in HeLa cervical cancer cells. Similar findings were also observed in HCT116, PC3, H1703 and H196 cells.
The deubiquitinating activity of both wild-type and mutant purified proteins is compared in vitro. Both wild-type and mutant enzymes were tested in dose-dependent reactions using the Ub-AMC assay (
| Number | Date | Country | Kind |
|---|---|---|---|
| 08291017.5 | Oct 2008 | EP | regional |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/IB2009/007458 | 10/29/2009 | WO | 00 | 6/6/2011 |
