Claims
- 1. In a method for determination of human bone alkaline phosphatase in the presence of human liver alkaline phosphatase using a differential wheat germ lectin precipitation the improvement which comprises employing as a standard an alkaline phosphatase which cannot be precipitated with wheat germ lectin to which phosphatase is covalently bound an ovomucoid carbohydrate of the formula ##STR4## wherein n is 0, 1, 2 or 3, A is an acyl radical containing 2 to 5 carbon atoms, R.sup.1 and R.sup.2 are each hydrogen atoms or hydroxyl groups, R.sup.3 is --COOH or --CH.sub.2 OH, R.sup.4 is a hydroxyl group or a --CHOH--CHOH--CH.sub.2 OH or --NH--CO--CH.sub.2 --CH(NH.sub.2)--COOH radical, or a complex containing said ovomucoid carbohydrate and wherein the binding provides for selective precipitability with wheat germ lectin to provide a standard compound for the determination of alkaline bone phosphate in the presence of alkaline liver phosphatase.
- 2. A method according to claim 1, wherein A is acetyl.
- 3. A method according to claim 1 wherein the carbohydrate is connected to the alkaline phosphatase via a bifunctional compound
- 4. A method according to claim 3, wherein the bifunctional compound is a homobifunctional imido ester or N-hydroxy-succinimide ester.
- 5. A method according to claim 3, wherein the bifunctional compound is glutardialdehyde.
- 6. A method according to claim 3 wherein the bifunctional compound is a heterobifunctional compound which contains at least one functional chemical group selected from the group consisting of N-hydroxysuccinimide, sulphhydryl, maleinamide, pyridylsulphide and halogen.
- 7. A method according to claim 1, wherein the alkaline phosphatase employed is obtained from calf intestine or from human placenta.
Priority Claims (1)
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3718753 |
Jun 1987 |
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Parent Case Info
This application is a continuation of application Ser. No. 201,523, filed Jun. 2, 1988 now abandoned.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
4681842 |
Rosalki |
Jul 1987 |
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4843001 |
Haug et al. |
Jun 1989 |
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Foreign Referenced Citations (1)
Number |
Date |
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0130708 |
Jan 1985 |
EPX |
Non-Patent Literature Citations (6)
Entry |
W. Behr et al., Arztl. Lab 32:159-165 (1986) (English translation). |
Stowoll et al. Advances in Carbohydrate Chemistry and Biochemistry 37:225, 1980. |
F. G. Lehmann (1980) Klin. Wochenschr. 58:947-951. |
Donald W. Moss (1982) Clin. Chem. 28/10:2007-2016. |
Yamamoto, K. et al. Biochem. 20:5894, 1981. |
Onica, D. et al. Clin. Chim. Acta 155:285, 1986. |
Continuations (1)
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201523 |
Jun 1988 |
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