Claims
- 1. A composition comprising at least one recombinase and at least two single-stranded targeting polynucleotides which are substantially complementary to each other and each having a consensus homology clamp for a gene family.
- 2. A composition according to claim 1 comprising at least one recombinase and a plurality of pairs of single stranded targeting polynucleotides which are substantially complementary to each other and each comprising a consensus homology clamp for a gene family, said plurality of pairs comprising a set of degenerate probes encoding the consensus sequence.
- 3. A composition according to claim 2 wherein said gene family is selected from the group consisting of the G-protein coupled receptor family, the AM-protein family, the bZIP transcription factor family, the mutS family, the recA family, the recF family, the Bcl-2 family, the single-stranded binding protein family; the TFIID transcription family, the TGF-beta family, the TNF family, the XPA family, the 14-3-3 family, and the XPG family.
- 4. A composition according to claim 2 wherein at least one of said polynucleotides further comprises an insertion sequence.
- 5. A composition according to claim 2 wherein at least one of said polynucleotides further comprises a purification tag.
- 6. A composition according to claim 2 wherein said targeting polynucleotides are coated with recombinase.
- 7. A composition according to claim 2 wherein said recombinase is a species of prokaryotic recombinase.
- 8. A composition according to claim 2 wherein said recombinase is a species of eukaryotic recombinase.
- 9. A kit comprising the composition of claim 2 and at least one reagent.
- 10. A method for targeting a sequence modification in at least one member of a consensus family of genes in a cell by homologous recombination, said method comprising introducing into at least one cell at least one recombinase and at least two single-stranded targeting polynucleotides which are substantially complementary to each other and each having a consensus homology clamp for said family.
- 11. A method according to claim 10 further comprising identifying a target cell having a targeted sequence modification.
- 12. A method according to claim 10 wherein at least one of said polynucleotides further comprises an insertion sequence.
- 13. A method according to claim 10 wherein at least one of said polynucleotides further comprises a purification tag.
- 14. A method according to claim 10 wherein said targeting polynucleotides are coated with recombinase.
- 15. A method according to claim 10 wherein said recombinase is a species of prokaryotic recombinase.
- 16. A method according to claim 10 wherein said recombinase is a species of eukaryotic recombinase.
- 17. A method according to claim 10 wherein the targeted sequence modification comprises the substitution of at least one nucleotide.
- 18. A method according to claim 10 wherein the targeted sequence modification comprises a plurality of substitutions.
- 19. A method according to claim 10 wherein said targeting polynucleotides are coated with said recombinase.
- 20. A method according to claim 10 wherein the recombinase and the targeting polynucleotides are introduced simultaneously.
Parent Case Info
[0001] This is a continuing application of U.S. Application No. 60/070,734, filed Dec. 11, 1997.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60070734 |
Dec 1997 |
US |
Divisions (1)
|
Number |
Date |
Country |
Parent |
09209676 |
Dec 1998 |
US |
Child |
10374466 |
Feb 2003 |
US |