Patent Application
20230397627
The present disclosure relates generally to the use of fat blends and emulsions of fat blends (such as water-in-oil fat blends) to improve certain features of a comestible product. In certain embodiments, certain water-in-oil emulsions disclosed herein can be suitably used to improve the organoleptic properties, the perceived texture, the perceived juiciness, or the mouthfeel of various foods, pet foods, or feed products, such as meat analogue products.
Meat products are widely consumed by humans and other animals, and continue to make up an increasing proportion of the caloric intake of humans worldwide. Even so, meat production is far less efficient than plant production on a calorie-per-land-area basis. Thus, increased meat consumption places a higher demand on the conversion of forests (such as rain forests) to arable land, and a concomitant increase in greenhouse gas emissions. Moreover, meat often contains high amounts of longer-chain saturated fats, which tend to have a deleterious effect on human health and well-being. But humans tend to enjoy the consumption of meat products, as the savory and satiating taste of meat products is often difficult to obtain from plant-based sources.
In recent years, there has been an increased effort to develop plant-based materials that exhibit many of the desirable features of meat-based food products. Such plant-based materials may be referred to as “meat analogues.” Despite significant efforts to develop such meat analogue technology, the resulting products only superficially resemble natural meat. Meat products contain a number of natural flavor compounds, and other similar such compounds are generated during the cooking process. Such compounds include fatty acids, as well as certain aroma compounds. Such compounds are often lacking from meat analogues, or are included in a way that created a very different taste experience for the consumer. The primary components of such meat analogues are typically plant protein and water. By contrast, many of the flavor compounds that contribute to meat's desirable taste are hydrophobic in nature. Therefore, even when present in meat analogues, are not delivered to the mouth in the same way. Thus, there is a continuing need to develop ways to improve meat analogue products, so that the experience of eating such products more closely simulates that of eating real meat products.
The present disclosure relates to the discovery of certain compositions that beneficially deliver certain aroma compounds in the presence of plant-based proteins that allows for improved juiciness, improved nutritional profile (for example, via a reduction in fat), improved masking of the undesirable taste or aroma of plant-based fibers or proteins. For example, it was discovered that one factor that leads to flavor imbalance in meat analogues is the selective binding of certain aroma compounds to plant proteins, which can create a distorted, non-culinary, non-authentic flavor profile for the product. To provide a partial solution to this problem, the present disclosure provides fat compositions, and emulsions thereof, that can suitably reduce the binding of lipophilic flavor compounds, such as aroma compounds, which often have higher log P values (for example, log P>1.5), as well as the binding of other compounds like thiols, disulfides, and the like, to the plant protein matrix of food products, pet food products, or feed product more particularly, meat analogues. Note that “log P” in this context refers to the base-10 log of the partition coefficient between water and 1-octanol.
By using the compositions disclosed herein in meat analogue products, one can improve one or more of the aroma profile, the taste profile, the sensory perception, and the texture perception of meat analogues, so as to more truly match such characteristics of real meat products, both during the consumption and the preparation (e.g., at-home cooking) of the meat analogue products.
The compositions disclosed herein can, when used in certain water-in-oil emulsions, reduce the binding of both hydrophilic and lipophilic compounds to the plant protein matrix of the meat analogue product. In some cases, by applying the flavor compounds in an emulsified format, certain flavored water-in-oil emulsion of the present disclosure can provide a more authentic flavor profile to that found in real met products, and, in some instances, without using a high concentration of the flavor compounds in the resulting meat analogue product. These features give one the opportunity to use less fat than what may be used in a comparable meat product, improve the perception of juiciness of a plant-based comestible product, or mask undesirable tastes or aromas of plant-based materials.
In a first aspect, the disclosure provides fatty compositions comprising solid fat particles and a liquid oil, wherein the solid fat particles are dispersed within the liquid (edible) oil. In some embodiments thereof, the fatty composition comprises an emulsifier. In some embodiments thereof, the fatty composition comprises one or more fat-soluble flavor compounds, fat-soluble aroma compounds, or combinations thereof. In some embodiments, the weight-to-weight ratio of liquid (edible) oil to solid fat particles ranges from 30:70 to 99:1. In some embodiments where an emulsifier is present, the emulsifier is present in a concentration ranging from 0.2 weight percent to 35 weight percent, based on the total weight of the fatty composition.
In a second aspect, the disclosure provides emulsions comprising a continuous phase and a dispersed phase, wherein one of the continuous phase or the dispersed phase comprises the fatty composition of the first aspect, and the other of the continuous phase or the dispersed phase comprises an aqueous medium. In some embodiments, the aqueous medium comprises water and, optionally, one or more water-soluble flavor compounds. In some embodiments thereof, the continuous phase comprises the fatty composition and the dispersed phase comprises an aqueous medium. In some further such embodiments, the continuous phase makes up from 30 weight percent to 99 weight percent of the emulsion, and the dispersed phase makes up from 0.1 weight percent to 50 weight percent of the emulsion.
In a third aspect, the disclosure provides uses of the fatty composition of the first aspect or the emulsion of the second aspect to improve the nutritional profile (for example, reduce the fat content) of a comestible article. In some embodiments, the comestible article is a food product, a pet food product, or a feed product. In some further embodiments, the comestible article is a meat analogue product. In some embodiments, the comestible article comprises one or more plant proteins, such as pea protein, soy protein, nut protein, and the like. In some further embodiments, the comestible article comprises one or more plant fibers, such as bamboo fiber, psyllium fiber, and the like. In some embodiments, the fatty composition or the emulsion are used in the comestible article at a concentration ranging from 0.1 weight percent to 10 weight percent, based on the total weight of the comestible article. In some embodiments, where the comestible article is a meat analogue, the meat analogue is a beef analogue, a poultry analogue, a fish analogue, a pork analogue, or a shellfish analogue, such as a crabmeat analogue, a scallop analogue, or a shrimp analogue. The disclosure also provides related methods of improving the nutritional profile of a comestible article.
In a fourth aspect, the disclosure provides uses of the fatty composition of the first aspect or the emulsion of the second aspect to increase the juiciness of a comestible article. In some embodiments, the comestible article is a food product, a pet food product, or a feed product. In some further embodiments, the comestible article is a meat analogue product. In some embodiments, the comestible article comprises one or more plant proteins, such as pea protein, soy protein, nut protein, and the like. In some further embodiments, the comestible article comprises one or more plant fibers, such as bamboo fiber, psyllium fiber, and the like. In some embodiments, the fatty composition or the emulsion are used in the comestible article at a concentration ranging from 0.1 weight percent to 10 weight percent, based on the total weight of the comestible article. In some embodiments, where the comestible article is a meat analogue, the meat analogue is a beef analogue, a poultry analogue, a fish analogue, a pork analogue, or a shellfish analogue, such as a crabmeat analogue, a scallop analogue, or a shrimp analogue. The disclosure also provides related methods of increasing the juiciness of a comestible article.
In a fifth aspect, the disclosure provides uses of the fatty composition of the first aspect or the emulsion of the second aspect to mask an undesirable aroma or an undesirable taste note of a comestible article. In some embodiments, the comestible article is a food product, a pet food product, or a feed product. In some further embodiments, the comestible article is a meat analogue product. In some embodiments, the comestible article comprises one or more plant proteins, such as pea protein, soy protein, nut protein, and the like. In some further embodiments, the comestible article comprises one or more plant fibers, such as bamboo fiber, psyllium fiber, and the like. In some embodiments, the fatty composition or the emulsion are used in the comestible article at a concentration ranging from 0.1 weight percent to 10 weight percent, based on the total weight of the comestible article. In some embodiments, where the comestible article is a meat analogue, the meat analogue is a beef analogue, a poultry analogue, a fish analogue, a pork analogue, or a shellfish analogue, such as a crabmeat analogue, a scallop analogue, or a shrimp analogue. The disclosure also provides related methods of reducing an undesirable aroma or taste note of a comestible article.
In a sixth aspect, the disclosure provides uses of the fatty composition of the first aspect or the emulsion of the second aspect to reduce the risk of rancidity of a comestible article. In some embodiments, the comestible article is a food product, a pet food product, or a feed product. In some further embodiments, the comestible article is a meat analogue product. In some embodiments, the comestible article comprises one or more plant proteins, such as pea protein, soy protein, nut protein, and the like. In some further embodiments, the comestible article comprises one or more plant fibers, such as bamboo fiber, psyllium fiber, and the like. In some embodiments, the fatty composition or the emulsion are used in the comestible article at a concentration ranging from 0.1 weight percent to 10 weight percent, based on the total weight of the comestible article. In some embodiments, where the comestible article is a meat analogue, the meat analogue is a beef analogue, a poultry analogue, a fish analogue, a pork analogue, or a shellfish analogue, such as a crabmeat analogue, a scallop analogue, or a shrimp analogue. The disclosure also provides related methods of reducing the rancidity of a comestible article. Other aspects and embodiments of the present disclosure are set forth below in the Detailed Description.
The following drawings are provided for purposes of illustrating various embodiments of the compositions and methods disclosed herein. The drawings are provided for illustrative purposes only, and are not intended to describe any preferred compositions or preferred methods, or to serve as a source of any limitations on the scope of the claimed inventions.
The following Detailed Description sets forth various aspects and embodiments provided herein. The description is to be read from the perspective of the person of ordinary skill in the relevant art. Therefore, information that is well known to such ordinarily skilled artisans is not necessarily included.
In at least one aspect, the present disclosure provides fatty compositions comprising solid fat particles and a liquid (edible) oil, wherein the solid fat particles are dispersed within the liquid (edible) oil.
The solid fat particles can be comprised of any suitable fat or fat mixture that is generally solid at room temperature, such as at 22° C. The fats in the fat particles can be from any suitable source, such as animal or plant sources.
In some embodiments, the fat particles comprise plant-derived fats. Suitable such plant-derived fats include cocoa butter, palm fat (i.e., solid palm oil), coconut fat (i.e., solid coconut oil), palm kernel fat (i.e., solid palm kernel oil), hydrogenated vegetable oils, or any combinations thereof. In some embodiments, the plant-derived fats include cocoa butter. In some such embodiments, the fat particles comprise at least 75% by weight, or at least 80% by weight, or at least 85% by weight, or at least 90% by weight, or at least 95% by weight, or at least 97% by weight, or at least 99% by weight, of plant-derived fats, based on the total weight of solid fat particles in the fatty composition.
In some other embodiments, the fat particles comprise animal-derived fats. Suitable such animal derived fats include butter, lard, tallow, or any combination thereof. In some such embodiments, the fat particles comprise no more than 25% by weight, or no more than 20% by weight, or no more than 15% by weight, or no more than 10% by weight, or no more than 5% by weight, or no more than 3% by weight, or no more than 1% by weight, of animal-derived fats, based on the total weight of solid fat particles in the fatty composition.
In general, the fats that make up the solid fat particles are triglycerides, but may include certain amounts of diglycerides or monoglycerides. Note, as used herein, the term “fat” refers to fatty acid glycerides, which are in a solid state at a given temperature, such as room temperature (22° C.). The fats that make up the solid fat particles comprise at least 75% by weight, or at least 80% by weight, or at least 85% by weight, or at least 90% by weight, or at least 95% by weight, or at least 97% by weight, of triglycerides, based on the total weight of fatty acid glycerides in the solid fat particles. The fatty acids that make up the fatty acid glycerides in the solid fat particles can be any suitable mixture of saturated and unsaturated fatty acids. In some embodiments, the fats that make up the solid fatty particles have an iodine number ranging from 1 to 75, or from 2 to 65, or from 5 to 55.
The solid fat particles can have any suitable melting point. In some embodiments, the solid fat particles have a melting point of at least 30° C., or at least 35° C., or at least 40° C. In some further embodiments, the solid fat particles have a melting point of no more than 80° C.
In some embodiments, the solid fat particles comprise an edible wax. Non-limiting examples of edible waxes include hydrogenated soy fat, palm fat, coconut fat, cocoa butter, carnauba wax, rice bran wax, shea butter, and mixture thereof. In some embodiments, the edible wax is an animal fat having higher melting point fat fractions such as palm or shea olein and mixtures thereof.
As noted above, the fatty composition also comprises a liquid (edible) oil into which the solid fat particles are dispersed. Any suitable can be used, so long as the oil is generally a liquid at room temperature (e.g., 22° C.), such as an animal oil, a fish oil, a vegetable oil, an algal oil, or any combination thereof. In some embodiments, the liquid oil is a plant-derived oil. In some other embodiments, the oil is not a plant-derived oil. Examples of liquid oils include sunflower oil, rapeseed or canola oil, soybean oil, palm oil, coconut oil, groundnut (peanut) oil, palm kernel oil, olive oil, cottonseed oil, sesame oil, linseed oil, an algal oil, a marine oil, avocado oil, argan oil, and any mixtures thereof. In some embodiments, the liquid oil comprises medium chain triglyceride oil (MCT) oil, soybean oil, cottonseed oil, peanut oil, sesame oil, corn oil, sunflower oil, canola oil, safflower oil, avocado oil, olive oil, argan oil, or any mixtures thereof.
In general, the liquid oil and the solid fatty particles will have a difference in their melting point. In general, the liquid oil will have a melting point of no more than 25° C., or no more than 20° C., or no more than 15° C., or no more than 10° C., or no more than 8° C., or no more than 5° C.
In some embodiments, the difference in melting between the higher melting point of the solid fat particles and the lower melting points of the liquid oil ranges from 5° C. to 105° C., or from 8° C. to 90° C., or from 10° C. to 80° C., or from 12° C. to 70° C., or from 15° C. to 60° C.
The solid fat particles and the liquid oil can be present in the fatty composition in any suitable relative amounts, so long as there is enough liquid oil to disperse the solid fatty particles at around room temperature. In some embodiments, weight ratio of liquid oil to solid fat particles in the fatty composition ranges from 30:70 to 99:1, or from 40:60 to 98:2, or from 55:45 to 97:3, or from 60:40 to 95:5, or from 70:30 to 93:7, or from 72:28 to 92:8.
In some embodiments, it may be desirable that the fatty composition be substantially free of animal fats or oils. Thus, in some embodiments, the fatty composition comprises no more than 5% by weight, or no more than 3% by weight, or no more than 1% by weight, or no more than 0.5% by weight, or no more than 0.3% by weight, or no more than 0.1% by weight, of animal-derived fats or oils, based on the total weight of the fatty composition.
In some embodiments, it may be desirable that the fatty composition be substantially free of fats or oils derived from genetically modified plants (GMO-derived fats or oils). Thus, in some embodiments, the fatty composition comprises no more than 5% by weight, or no more than 3% by weight, or no more than 1% by weight, or no more than 0.5% by weight, or no more than 0.3% by weight, or no more than 0.1% by weight, of GMO-derived fats or oils, based on the total weight of the fatty composition.
In some embodiments, the fatty composition comprises an emulsifier. When present, the emulsifier can be present at any suitable concentration. In some embodiments, the concentration of emulsifier in the fatty composition ranges from 0.2% by weight to 35% by weight, or from 0.3% by weight to 20% by weight, or from 0.4% by weight to 15% by weight, or from 0.5% by weight to 10% by weight, or from 0.6% by weight to 8% by weight, of emulsifier, based on the total weight of the fatty composition.
In general, emulsifiers are amphiphilic molecules that concentrate at the interface between two phases and modify the properties of that interface. Suitable non-limiting examples of emulsifiers are described in MCCUTCHEON'S EMULSIFIERS & DETERGENTS OR THE INDUSTRIAL SURFACTANTS HANDBOOK. Some specific non-limiting examples of emulsifiers include lecithins, polyoxyethene, stearates, polysorbate 20, sorbitan derivatives (polysorbate 20, polysorbate 80, polysorbate 40, polysorbate 60, and polysorbate 65), mixed ammonium salts of phosphorylated glycerides, enzymatically hydrolyzed carboxymethyl-cellulose, mono- and diglycerides of fatty acids, esters of mono- and diglycerides of fatty acids (such as acetic acid esters, lactic acid esters, citric acid esters, tartaric acid esters, mono- and diacetyl tartaric acid esters, mixed acetic and tartaric acid esters), succinylated monoglycerides, sucrose esters of fatty acids, sucroglycerides, polyglycerol esters of fatty acids, polyglycerol polyricinoleate, propane-1,2-diol esters of fatty acids, propylene glycol esters of fatty acids, lactylated fatty acid esters of glycerol and propanol, thermally oxidized soya bean oil interacted with mono- and diglycerides of fatty acids, sodium stearoyl lactylate, calcium stearoyl lactylate, stearyl tartrate, stearyl citrate, sodium stearoyl fumarate, calcium stearoyl fumarate, sodium dodecyl sulfate, ethoxylated mono- and di-glycerides, methyl glucoside-coconut oil ester, sorbitan monostearate, sorbitan tristearate, sorbitan monolaurate, sorbitan monooleate, sorbitan monopalmitate, sorbitan trioleate, and combinations thereof.
In some embodiments, the emulsifier comprises lecithins (such as mixtures of glycerophospholipids, including phosphatidylcholine PC, phosphatidylethanolamine PE, phosphatidylinositol PI, and phosphatidic acid PA) with different triglyceride content (pure lecithins or deoiled lecithins, different ratio PC-to-PE-to-PI). Such lecithins can be used in any suitable form, including in the form of oily paste or powders. Lecithins are commercially available from a number of suppliers including Cargill (brands EMELPUR, EMULTOP, LECIMULTHIN, EPIKURON), Archer Daniels Midland (brand ULTRALEC, ADLEC), Solae (brand SOLEC), and Bunge (brand BUNGEMAXX).
Suitable emulsifiers can be characterized according to their hydrophilic-lipophilic balance (HLB), measured on an empirical scale set forth in Griffin, J. C
In some embodiments, the emulsifier present in the fatty composition has an HLB value of no more than 10, of no more than 9, or of no more than 8, or of no more than 7, or of no more than 6. In some embodiments, the emulsifier present in the fatty composition has an HLB value ranging from 3 to 9, or from 4 to 9, or from 5 to 9, or from 6 to 9, or from 7 to 9, or from 3 to 8, or from 3 to 7, or from 3 to 6. Such emulsifiers may be referred to as low-HLB emulsifiers.
Non-limiting examples of the low-HLB emulsifiers suitable to form water-in-oil emulsion include, alcohol alkoxylates, alkylamine alkoxylates, polyetheramine alkoxylates, ethylene oxide/propylene oxide block polymers, phosphate esters, alkyl sulfates, alkyl ether sulfates, alkyl and alkylbenzene sulfonates, fatty acid esters, fatty oil alkoxylates, saccharide derivatives, sorbitan derivatives, alkyl phenol alkoxylates, arylphenol alkoxylates, sulphosuccinates, sulphosuccinamates, and any combinations thereof. The emulsifiers can be nonionic, anionic, cationic or zwitterionic. In some embodiments, the emulsifiers are suitable for use in foods, pet foods, or feed products, including, but not limited to fatty acid esters, saccharide derivatives, sorbitan derivatives, especially sorbitan esters, mono/diglyceride, citric acid esters, lecithin and other phospholipids, and combinations thereof. Commercial examples of such low-HLB emulsifiers include, but are not limited to, the DIMODAN emulsifiers (distilled monoglycerides) available from DuPont-Danisco, CITREM (citric acid esters of mono- and diglycerides) available from Paalsgard, SOLEC (soy lecithin) available from DuPont Nutrition, or GRINSTED STS/SMS (sorbitan esters) also available from DuPont Nutrition.
In some embodiments thereof, the fatty composition comprises fat-soluble flavor compounds, such as fat-soluble aroma compounds. Such fat-soluble flavor compounds can be present in the fatty composition in any suitable amount. For example, in some embodiments, the fat-soluble flavor compounds make up from 0.1% by weight to 80% by weight, or from 1% by weight or from 60% by weight, of the fatty composition, based on the total weight of the fatty composition.
Any suitable fat-soluble flavor compounds can be used, according to those known in the relevant art. Flavor compounds are discussed in further detail below.
In another aspect, the disclosure provides emulsions comprising a continuous phase and a dispersed phase, wherein one of the continuous phase or the dispersed phase comprises the fatty composition of the foregoing aspect and any embodiments thereof, and the other of the continuous phase or the dispersed phase comprises an aqueous medium.
In some embodiments, the continuous phase comprises the fatty composition and the dispersed phase comprises the aqueous medium. The fatty composition can have any suitable characteristics, according to the embodiments set forth in the preceding section of this disclosure. The aqueous medium comprises water. For example, in some embodiments, water makes up at least 75% by weight, or at least 80% by weight, or at least 85% by weight, or at least 90% by weight, or at least 95% by weight, or at least 97% by weight, or at least 99% by weight, of the aqueous medium, based on the total weight of aqueous medium. In some further embodiments, the aqueous medium comprises water-soluble flavor compounds.
AS used herein, the term “emulsion” refers to a mixture of two or more liquids that are normally immiscible (i.e., not mixable). In an emulsion, one liquid (the dispersed phase) is dispersed in the other (the continuous phase). In certain instances, one of the phases is a hydrophobic or lipophilic phase, and the other phase is a hydrophilic phase. In certain embodiments disclosed herein, the emulsion is a water-in oil emulsions, which comprises a continuous hydrophobic (i.e., lipophilic) phase in which the hydrophilic phase is dispersed.
The emulsion can be any type of emulsion. For example, in some embodiments, the emulsion is a macroemulsion, a microemulsion, or a nanoemulsion.
The emulsions disclosed herein may be prepared by any suitable procedure. For example, in some embodiments, the emulsion is prepared by applying mechanical force to emulsify the disperse phase droplets, such as by mechanical mixing with a high shear blender, a colloidal mill, an impeller mixer, or by the use of a high-pressure homogenizer. In some embodiments, such emulsions are prepared by ultrasound processing, by phase inversion emulsification, by membrane emulsification, or by emulsification using microfluidic channels.
The emulsion can have any suitable weight ratio between the continuous phase and the dispersed phase. For example, in some embodiments, the continuous phase makes up from 30% by weight to 99% by weight, or from 35% by weight to 98% by weight; or from 38% by weight to 97% by weight, or from 40% by weight to 95% by weight, or from 45% by weight to 93% by weight, of the emulsion, based on the total weight of the emulsion. In certain related embodiments, the dispersed phase makes up from 0.1% by weight to 50% by weight, or from 3% by weight to 45% by weight, or from 5% by weight to 40% by weight, or from 8% by weight to 30% by weight, of the emulsion, based on the total weight of the emulsion.
In some embodiments, the aqueous medium comprises soluble fiber. In such embodiments, the soluble fiber can be present in any suitable concentration. For example, in some embodiments, the soluble fiber is present in the aqueous medium at a concentration ranging from 0.1% by weight to 5% by weight, or from 1% by weight to 2% by weight, based on the total weight of the aqueous medium. In some embodiments, the soluble fiber is present at a concentration suitable for forming a hydrogel when the emulsion is heated above room temperatures, such as standard temperatures for cooking meat.
As used herein, the term “soluble fiber” refers to polysaccharides that are soluble in water, such as according to the method set forth in Prosky et al, J. A
In some embodiments, the aqueous medium comprises water-soluble flavor compounds. Any suitable water-soluble flavor compounds can be used, according to those known in the relevant art. Flavor compounds are discussed in further detail below.
In some cases, it may be desirable to describe the resulting emulsion in terms of particular physical characteristics. For example, in some embodiments, the emulsion is a water-in-oil emulsion having an elastic shear modulus G′ (0.3%, 0.5 Hz) higher than its viscous shear modulus G″ (0.3%, 0.5 Hz). Using this notation, the numbers provided in brackets refer to the strain amplitude given in percent values, and the frequency of the oscillatory shearing, meaning that values given refer to the shear modulus measured using shear oscillations performed at a frequency of 0.5 Hz and a strain amplitude of 0.3%. The elastic shear modulus represents the elastic behavior of a material for a given frequency and strain amplitude, and is conventionally written as G′ and measured in units of Pascal (Pa). The viscous shear modulus represents the viscous behavior of a material for a given frequency and strain amplitude, and is conventionally written as G″ and also measured in units of Pascal (Pa). These characteristic values are, for example, defined in R. G. Larson, T
These viscoelastic properties are measured during dynamic tests under oscillating shear strains (small deformations) performed at a constant temperature or between range of temperatures, for example at temperatures ranging from 4° C. to 80° C., and at a constant frequency (i.e., 0.5 Hz) or a frequency range on a rheometer (for example, a Model DHR-2, TA Instruments) under a torsional/shear strain (i.e., a sinusoidally varying shear strain with a strain amplitude of 0.3% and a frequency of 0.5 Hz), or a range of torsional/shear strains, for example testing a range of oscillatory shear strains with amplitudes ranging from 0.1% to 100%, for example, in cone-plate geometry (for example with a 40 mm diameter cone/plate geometry and a 2 degree cone angle). Such methods are further described in P. Fischer et al., “Rheology of Food Materials”, in CURRENT OPINION IN COLLOID & INTERFACE SCIENCE, vol. 16(1), pp. 36-40 (2011). For example, G′ (0.3%, 0.5 Hz) is the elastic shear modulus of a material, measured at a frequency of 0.5 Hz and at a torsional/shear stress of 0.3%, for a temperature from 5° C. to 80° C. G′ (18° C., 0.5 Hz) is the elastic shear modulus of a material, measured at a frequency of 0.5 Hz and at a temperature of 18° C., for any torsional/shear stress from 0.1% to 100%. G″ (0.3%, 0.5 Hz) is the viscous shear modulus of a material, measured at a frequency of 0.5 Hz and at a torsional/shear stress of 0.3%, for any temperature from 5° C. to 80° C.
In some embodiments, the emulsions disclosed herein have an elastic shear modulus G′ (0.5 Hz, 37° C.) higher than the viscous shear modulus G″ (0.5 Hz, 37° C.) at a shear strain lower than 8%, or lower than 7%, or lower than 5%. In some embodiments, the emulsions disclosed herein have a ratio G′ (0.3%, 0.5 Hz)/G′ (0.3%, 0.5 Hz) of no more than 20, or no more than 15, or no more than 10, or no more than 5, or no more than 3, or no more than 2, or no more than 1, or no more than 0.5. In some embodiments, the emulsions disclosed herein have a ratio G′ (0.3%, 0.5 Hz)/G′ (0.3%, 0.5 Hz) of at least 0.001, or at least 0.01, or at least 0.05. In some embodiments, the emulsions disclosed herein have a ratio G′/G″ (0.5 Hz, 18° C.) of no more than 1. In some embodiments, the emulsions disclosed herein have a ratio G′/G″ (0.5 Hz, 18° C.) of at least 0.01.
The dispersed phase generally forms drops in the emulsion. The drop size can be any suitable size, depending on various factors. In some embodiments, the emulsions have a drop size having an average diameter of ranging from 0.1 μm to 30 μm, or from 0.8 μm to 20 μm, or from 2 μm to 10 μm. The drop size can be measured via any well-established method that allows measurements which are accurate within an experimental error of 5% at the most and preferably below 1%. Suitable well-established methods use light microscopy (for example, R. J. Hunter, I
The emulsions disclosed herein can, in certain embodiments, contain other additives, adjuvants, and the like, that are commonly included in food products, pet food products, and feed products. For example, the emulsions disclosed herein can, in certain embodiments, comprise any additional ingredients or combination of ingredients as are commonly used in comestible products, including, but not limited to:
One example of an additional ingredient includes free fatty acids. Suitable examples include stearic acid, palmitic acid, myristic acid, lauric acid, capric acid, caprylic acid, and the like. These free fatty acids can be present at any suitable concentration. For example, in some embodiments, free fatty acids are present at a concentration ranging from 3% by weight to 20% by weight, or from 5% by weight to 18% by weight, or from 7% by weight to 16% by weight, in the emulsion, based on the total weight of the emulsion.
Other acids can also be present, for example, to help adjust the pH of the final product. Suitable acids for this purpose include comestible acids, such as lactic acid, citric acid, and combinations thereof. Such acids can be present at any concentration. For example, in some embodiments, free fatty acids are present at a concentration ranging from 0.2% by weight to 3.0% by weight, or from 0.5% by weight to 2.0% by weight, or from 0.7% by weight to 1.8% by weight, in the emulsion, based on the total weight of the emulsion.
As noted above, both the fatty composition and the aqueous medium can, in certain embodiments, comprise fat-soluble and water-soluble flavour compounds, respectively. AS used herein, a “flavor” or “flavor compound” that are added, either alone or in combination with other such compounds, to a comestible composition to impart, improve, or modify its organoleptic properties, in particular its flavor, taste, or aroma. Such compounds may be natural or synthetic. Many such flavor compounds are listed in reference texts such as S. Arctander, P
Non-limiting examples of flavor compounds that may be used in the fatty compositions or emulsions disclosed herein include organic salts, inorganic salts, organic acids, sugars, amino acids and their salts (such as glutamates or aspartates), ribonucleotides, and sources thereof, and any combination of the foregoing.
Sweeteners are a common example of flavour compounds. Thus, in some embodiments, the fatty compositions or emulsions disclosed herein comprise an additional sweetener, such as a caloric sugar, such as sucrose, glucose, fructose (e.g., in the form of high-fructose corn syrup), or any combination thereof. In some embodiments, the sweetening composition comprises one or more rebaudiosides. In some embodiments, the fatty compositions or emulsions disclosed herein comprises one or more high-intensity artificial sweeteners, such as acesulfame potassium, sucralose, aspartame, cyclamate, neotame, and the like. In some other embodiments, the fatty compositions or emulsions disclosed herein comprise one or more low-calorie carbohydrates or sugar alcohols, such as allulose, xylitol, erythritol, and the like. In some other embodiments, the fatty compositions or emulsions disclosed herein comprise mogrosides, for example, as monk fruit juice or extract, or as one or more of mogroside III, mogroside IV, mogroside V, siamenoside I, isomogroside V, mogroside IVE, isomogroside IVE, isomogroside IV, mogroside IIIE, 11-oxomogroside V, the 1,6-alpha isomer of siamenoside I, and any combinations thereof. Additional mogroside compounds that may be suitably included in the sweetening composition are described in U.S. Patent Application Publication No. 2017/0119032.
Various other sweeteners may also be included in the fatty compositions or emulsions disclosed herein. Non-limiting examples include D-psicose, L-ribose, D-tagatose, L-glucose, L-fucose, L-arbinose, D-turanose, D-leucrose, isomalt, lactitol, mannitol, sorbitol, maltodextrin, saccharin, alitame, cyclamic acid, tagatose, maltose, galactose, mannose, lactose, D-tryptophan, glycine, maltitol, lactitol, isomalt, hydrogenated starch hydrolyzate (HSH), chemically modified mogrosides (such as glucosylated mogrosides), carrelame and other guanidine-based sweeteners, honey, Jerusalem artichoke syrup, licorice root, luo han guo (fruit, powder, or extracts), lucuma (fruit, powder, or extracts), maple sap (including, for example, sap extracted from Acer saccharum, Acer nigrum, Acer rubrum, Acer saccharinum, Acer platanoides, Acer negundo, Acer macrophyllum, Acer grandidentatum, Acer glabrum, Acer mono), maple syrup, maple sugar, walnut sap (including, for example, sap extracted from Juglans cinerea, Juglans nigra, Juglans ailatifolia, Juglans regia), birch sap (including, for example, sap extracted from Betula papyrifera, Betula alleghaniensis, Betula lenta, Betula nigra, Betula populifolia, Betula pendula), sycamore sap (such as, for example, sap extracted from Platanus occidentalis), ironwood sap (such as, for example, sap extracted from Ostrya virginiana), mascobado, molasses (such as, for example, blackstrap molasses), molasses sugar, monatin, monellin, cane sugar (also referred to as natural sugar, unrefined cane sugar, or sucrose), palm sugar, panocha, piloncillo, rapadura, raw sugar, rice syrup, sorghum, sorghum syrup, cassava syrup (also referred to as tapioca syrup), thaumatin, yacon root, malt syrup, barley malt syrup, barley malt powder, beet sugar, cane sugar, crystalline juice crystals, caramel, carbitol, carob syrup, castor sugar, hydrogenated starch hydrolates, hydrolyzed can juice, hydrolyzed starch, invert sugar, anethole, arabinogalactan, arrope, syrup, P-4000, acesulfame potassium (also referred to as acesulfame K or ace-K), alitame (also referred to as aclame), advantame, aspartame, baiyunoside, neotame, benzamide derivatives, bernadame, canderel, carrelame and other guanidine-based sweeteners, vegetable fiber, corn sugar, coupling sugars, curculin, cyclamates, cyclocarioside I, demerara, dextran, dextrin, diastatic malt, dulcin, sucrol, valzin, dulcoside A, dulcoside B, emulin, enoxolone, maltodextrin, saccharin, estragole, ethyl maltol, glucin, gluconic acid, glucono-lactone, glucosamine, glucoronic acid, glycerol, glycine, glycyphillin, glycyrrhizin, glycyrrhetic acid monoglucuronide, golden sugar, yellow sugar, golden syrup, granulated sugar, gynostemma, hernandulcin, isomerized liquid sugars, jallab, chicory root dietary fiber, kynurenine derivatives (including N′-formyl-kynurenine, N′-acetyl-kynurenine, 6-chloro-kynurenine), galactitol, litesse, ligicane, lycasin, lugduname, guanidine, falernum, mabinlin I, mabinlin II, maltol, maltisorb, maltodextrin, maltotriol, mannosamine, miraculin, mizuame, mogrosides (including, for example, mogroside IV, mogroside V, and neomogroside), mukurozioside, nano sugar, naringin dihydrochalcone, neohesperidine dihydrochalcone, nib sugar, nigero-oligosaccharide, norbu, orgeat syrup, osladin, pekmez, pentadin, periandrin I, perillaldehyde, perillartine, petphyllum, phenylalanine, phlomisoside I, phlorodizin, phyllodulcin, polyglycitol syrups, polypodoside A, pterocaryoside A, pterocaryoside B, rebiana, refiners syrup, rub syrup, rubusoside, selligueain A, shugr, siamenoside I, siraitia grosvenorii, soybean oligosaccharide, Splenda, SRI oxime V, steviol glycoside, steviolbioside, stevioside, strogins 1, 2, and 4, sucronic acid, sucrononate, sugar, suosan, phloridzin, superaspartame, tetrasaccharide, threitol, treacle, trilobtain, tryptophan and derivatives (6-trifluoromethyl-tryptophan, 6-chloro-D-tryptophan), vanilla sugar, volemitol, birch syrup, aspartame-acesulfame, assugrin, and combinations or blends of any two or more thereof.
In some embodiments, the fatty compositions or emulsions set forth herein include compounds that impart an umami, a kokumi taste, or a salty taste, including, but not limited to, amino acids (such as glutamic acid and aspartic acid), glutamate salts (such as monosodium glutamate), aspartate salts, yeast, fermented products, garlic or extracts thereof, oligopeptides (such as glutathione or other gamma-glutamyl tripeptides), and purinic ribonucleotides (such as inosine monophosphate (IMP) and guanosine monophosphate (GMP)), sodium chloride, and potassium chloride.
The fatty compositions or emulsions set forth according to any of the foregoing embodiments, also include, in certain embodiments, one or more additional flavor-modifying compounds, such as compounds that enhance sweetness (e.g., hesperetin, naringenin, phloretin, rhoifolin, etc.), compounds that block or mask bitterness, compounds that enhance umami, compounds that reduce sourness or licorice taste, compounds that enhance saltiness, compounds that enhance a cooling effect, compounds that enhance mouthfeel, or any combinations of the foregoing.
In some embodiments, fatty compositions or emulsions disclosed herein comprise one or more sweetness enhancing compounds. Such sweetness enhancing compounds include, but are not limited to, naturally derived compounds, such as hesperitin, naringenin, rhoifolin, phloretin, glucosylated natural steviol glycosides, licorice-derived glucuronates, aromadendrin-3-O-acetate, or other like flavonols, or flavonoids, or synthetic compounds, such as any compounds set forth in U.S. Pat. Nos. 8,541,421; 8,815,956; 9,834,544; 8,592,592; 8,877,922; 9,000,054; and 9,000,051, as well as U.S. Patent Application Publication No. 2017/0119032. Some suitable examples include: 3-((4-amino-2,2-dioxo-1H-benzol[c][1,2,6]thiadiazin-5-yl)oxy)-2,2-dimethyl-N-propyl-propanamide, N-(1-((4-amino-2,2-dioxo-1H-benzo[c] [1,2,6]-thiadiazin-5-yl)oxy)-2-methyl-propan-2-yl)-isonicotinamide, or any combination thereof.
In some further embodiments, fatty compositions or emulsions disclosed herein comprise one or more umami or kokumi enhancing compounds. Such umami enhancing compounds include, but are not limited to, naturally derived compounds, such as ericamide, or synthetic compounds, such as any compounds set forth in U.S. Pat. Nos. 8,735,081; 8,124,121; and 8,968,708.
In some further embodiments, fatty compositions or emulsions disclosed herein comprise one or more cooling enhancing compounds. Such cooling enhancing compounds include, but are not limited to, naturally derived compounds, such as menthol or analogs thereof, or synthetic compounds, such as any compounds set forth in U.S. Pat. Nos. 9,394,287 and 10,421,727.
In some further embodiments, fatty compositions or emulsions disclosed herein comprise one or more bitterness blocking compounds. Such bitterness blocking compounds include, but are not limited to, naturally derived compounds, such as menthol or analogs thereof, or synthetic compounds, such as any compounds set forth in U.S. Pat. Nos. 8,076,491; 8,445,692; and 9,247,759, and in PCT Publication No. WO 2020/033669.
In some further embodiments, fatty compositions or emulsions disclosed herein comprise one or more mouthfeel modifying compounds. Such mouthfeel modifying compounds include, but are not limited to, tannins, cellulosic materials, bamboo powder, and the like.
In some further embodiments, fatty compositions or emulsions disclosed herein comprise one or more flavor masking compounds. Such flavor masking compounds include, but are not limited to, cellulosic materials, materials extracted from fungus, materials extracted from plants, citric acid, carbonic acid (or carbonates), and the like.
The flavor composition may be water soluble or oil soluble. Depending on its solubility, the flavor composition may be in the dispersed phase and/or the continuous phase. Solubility of a flavor composition can be evaluated according to dissolution in water or oil notably using the partition coefficient (P) (Log P value).
In some instances, the flavouring compounds can be compounds that simulate the flavour properties of blood, such as the blood typically found in red meat products, such as beef, lamb, pork, and the like. Such flavour compounds or combinations of flavour compounds can be used in imitation burgers, and the like. Such flavour compounds are often plant-based metalloproteins, which are formed from proteins and iron to mimic heme.
In another aspect, the disclosure provides methods of making the emulsion of the previous aspect (and any embodiments thereof), the method comprising: (a) providing a fatty composition (as described in any of the preceding embodiments); (b) emulsifying an aqueous medium (according to any of the preceding embodiments) as the dispersed phase into the fatty composition as the continuous phase at a temperature above the phase transition temperature of the continuous phase to form an emulsified composition, wherein the fatty composition optionally comprises an emulsifier; and (c) cooling the emulsified composition to a temperature below the phase change temperature of the continuous phase.
The term “phase transition temperature” means the temperature at which the medium (or the continuous phase) changes, for example, from solid to liquid when it is referred to melting temperature or melting point.
In some embodiments, the method comprises a preheating step of heating the fatty composition to a temperature ranging from 80° C. to 150° C., or to a temperature ranging from 95° C. to 120° C.
In some embodiments, the method comprises a preheating step of heating the aqueous medium to temperature ranging from 60° C. to 100° C., or to a temperature ranging from 70° C. to 90° C.
In some embodiments, the cooling step (c) is carried out by reducing temperature of the emulsion at a rate ranging from 5° C./hour to 30° C./hour, or at a rate ranging from 10° C./hour to 25° C./hour, or at a rate ranging from 12° C./hour to 15° C./hour.
In certain aspects, the disclosure provides uses of the fatty composition or the emulsion disclosed herein to improve the nutritional profile (for example, reduce the fat content) of a comestible article. In some embodiments, the fatty composition or the emulsion are used in the comestible article at a concentration ranging from 0.1 weight percent to 10 weight percent, based on the total weight of the comestible article. In some embodiments, where the comestible article is a meat analogue, the meat analogue is a beef analogue, a poultry analogue, a fish analogue, a pork analogue, or a shellfish analogue, such as a crabmeat analogue, a scallop analogue, or a shrimp analogue. In certain embodiments, the fat content of the comestible article is reduced by at least 5%, or at least 10%, or at least 15%, or at least 20%, or at least 25%, relative to a comparable comestible article not employing the fatty emulsions disclosed herein.
In certain other aspects, the disclosure provides uses of the fatty composition or the emulsion disclosed herein to increase the juiciness of a comestible article. In some embodiments, the fatty composition or the emulsion are used in the comestible article at a concentration ranging from 0.1 weight percent to 10 weight percent, based on the total weight of the comestible article. In some embodiments, where the comestible article is a meat analogue, the meat analogue is a beef analogue, a poultry analogue, a fish analogue, a pork analogue, or a shellfish analogue, such as a crabmeat analogue, a scallop analogue, or a shrimp analogue.
In certain other aspects, the disclosure provides uses of the fatty composition or the emulsion disclosed herein to reduce an undesirable aroma or an undesirable taste note of a comestible article, wherein the comestible article comprises a plant protein, a plant fiber, or a combination thereof. In some embodiments, the fatty composition or the emulsion are used in the comestible article at a concentration ranging from 0.1 weight percent to 10 weight percent, based on the total weight of the comestible article. In some embodiments, where the comestible article is a meat analogue, the meat analogue is a beef analogue, a poultry analogue, a fish analogue, a pork analogue, or a shellfish analogue, such as a crabmeat analogue, a scallop analogue, or a shrimp analogue.
In certain other aspects, the disclosure provides uses of the fatty composition or the emulsion disclosed herein to reduce a risk of rancidity of a comestible article, wherein the comestible article comprises a plant protein, a plant fiber, or a combination thereof. In some embodiments, the fatty composition or the emulsion are used in the comestible article at a concentration ranging from 0.1 weight percent to 10 weight percent, based on the total weight of the comestible article. In some embodiments, where the comestible article is a meat analogue, the meat analogue is a beef analogue, a poultry analogue, a fish analogue, a pork analogue, or a shellfish analogue, such as a crabmeat analogue, a scallop analogue, or a shrimp analogue.
In certain related aspects, the disclosure provides corresponding methods to the uses set forth immediately above.
The foregoing aspects are specifically directed to comestible articles or directed to uses or methods that concern comestible articles. Such comestible articles can be any edible product, such as a food product, a pet food product, or a feed product.
In some further embodiments, the comestible article is a meat analogue product. The meat analogue is a non-meat product that, when eaten, is intended to simulate the sensory experience of eating meat. In some embodiments, the meat analogue product is a beef analogue product, a poultry analogue product, a fish analogue product, a pork analogue product, or a shellfish analogue product, such as a crabmeat analogue product, a scallop analogue product, or a shrimp analogue product. Non-limiting examples include vegetarian burgers, sausage, imitation chicken nuggets, imitation deli meat, imitation poultry, imitation beef, imitation pork, imitation ham, imitation fresh sausage or imitation raw meat preparations, imitation cured meat products, and imitation reformed meat.
The fatty composition (of any of the preceding embodiments) or emulsion (of any of the preceding embodiments) can be present in the comestible article at any suitable concentration. In some embodiments, the fatty composition or the emulsion are present in the comestible article at a concentration ranging from 0.01% by weight to 10% by weight, or from 0.1% by weight to 10% by weight, or from 0.5% by weight to 5% by weight, based on the total weight of the comestible article.
The fatty composition or emulsion can be introduced to the comestible article in any suitable manner. In some embodiments, for example, the fatty composition or the emulsion is added to the comestible article by injection, vacuum tumbling (optionally with a carrier material), or mixing with the food prior to its preparation (for example, before its baking, its extrusion, and the like).
As noted above, one goal of using the fatty compositions or the emulsions described herein is to allow one to reduce the amount of animal products, such as animal fats, in the comestible article. In some embodiments, the comestible article comprises no more than 1% by weight, or no more than 0.5% by weight, or no more than 0.1% by weight, or no more than 0.05% by weight, or no more than 0.01% by weight of animal-derived fatty acid glycerides.
Note that the meat analogue products may be contained within or mixed with other non-meat products. Thus, the presently disclosed flavored products (e.g., meat analogues) can be included in pasta sauces, in soups, in marinades or pastes, notably used for fish or meat products, in confectionery products, and in dairy products.
In some embodiments, the comestible article comprises one or more plant proteins, such as pea protein, soy protein, nut protein, and the like. In some further embodiments, the comestible article comprises one or more plant fibers, such as bamboo fiber, psyllium fiber, and the like. In some embodiments, the fatty composition or the emulsion are used in the comestible article at a concentration ranging from 0.1 weight percent to 10 weight percent, based on the total weight of the comestible article.
In some embodiments, the flavored product further comprises proteins, such as proteins derived from plants, animals, eggs, dairy products, and the like. In some embodiments, the proteins comprise at least 75% by weight, or at least 80% by weight, or at least 85% by weight, or at least 90% by weight, or at least 95% by weight, or at least 97% by weight, or at least 99% by weight, of plant-derived protein, based on the total weight of protein in the comestible article. Any suitable plant protein or blend of plant proteins can be used. Non-limiting examples include soy protein, corn protein, pea protein, canola protein, sunflower protein, sorghum protein, rice protein, amaranth protein, potato protein, tapioca protein, arrowroot protein, chickpeas protein, lupin protein, wheat protein, oat protein, rye protein, barley protein, bean or lentil protein, protein from fermented soy products (such as tofu, tempeh, etc.), peanut protein, cashew protein, nut protein (such as almond protein, walnut protein, and the like), quinoa protein, mycoprotein, chia protein, hemp protein, pumpkin seed protein, spirulina protein, broccoli protein, kale protein, brussels sprout protein, and any mixtures thereof. In some embodiments, the proteins comprise pea protein.
The comestible article can also comprise fiber or blends of fiber. In general, such fiber is derived from plant materials. In general, plant-based fibers are mainly composed of non-starch polysaccharides and other plant components such as cellulose, resistant starch, resistant dextrins, inulin, lignins, chitins (in fungi), pectins, beta-glucans, and various oligosaccharides. Non-limiting examples of suitable plant sources for fiber include legumes (peas, soybeans, lupins, and other beans), oats, rye, chia, barley, fruit (figs, avocados, plums, prunes, berries, bananas, apples, quinces, kiwi, grapes, tomatoes, and pears), vegetables (broccoli, carrots, green beans, cauliflower, zucchini, celery, nopal, and artickokes), root tubers/vegetables (sweet potatoes and onions), psyllium seed husks, flax seeds, nuts (almonds), whole grains, wheat bran, corn bran, seeds, potato skins, lignans, and any combinations thereof.
Due to its high stability to shear and temperature of the emulsions disclosed herein, the flavored emulsion of the invention is particularly suitable for extruded and/or baked food, pet-food or feed products more particularly comprising animal and/or vegetable proteins. In some cases, the extruded and/or baked food, pet-food or feed products may be selected among meat- and/or fish-based food or analogue and mixtures thereof (in other words, meat-based food and/or fish-based food or meat analogue or fish analogue and mixtures thereof); extruded and/or baked food, meat analogue or extruded and/or baked food fish analogue are preferred. Non-limiting examples of extruded and/or baked food, pet-food or feed products are snack products or extruded vegetable proteins with the aim to texture the protein from which meat analogous (e.g. burgers) are prepared from. The flavored emulsion can be added pre-extrusion or after extrusion to either, the non-extruded vegetable protein isolate/concentrate or to the textured vegetable protein from which a burger or nugget (etc.) can be formed.
The fatty composition or emulsions disclosed herein can be used in a wide variety of edible end-products. End-products are more particularly a food, pet-food, or feed product. The fatty composition or emulsions disclosed herein are particularly advantageous for vegetarian meat analogues or meat replacers, vegetarian burger, sausages, patties, imitation chicken nuggets, and the like. Meat, for the purpose of the present disclosure, encompasses red meat (such as beef, pork, mutton, lamb, and venison) and poultry (such as chicken, turkey, goose and duck), as well as fish and shellfish. In some embodiments, the comestible article is a beef analogue, a poultry analogue, or a pork analogue.
Due to the resistance to shearing and high temperatures, the emulsions disclosed herein can also be of particular interest in the following examples of products:
Unless stated otherwise, percentages (%) are meant to designate a percentage by weight of a composition.
It should be understood that the total amount of ingredients in the composition or emulsion is 100%.
Unless specified otherwise, numerical ranges expressed in the format “from x to y” are understood to include x and y. When for a specific feature multiple preferred ranges are described in the format “from x to y,” it is understood that all ranges combining the different endpoints are also contemplated.
The term “comprise” or “comprising”, for the purpose of the present invention is intended to mean “including”. It is not intended to mean, “consisting only of”.
A quantity of 143 g of an edible oil (MCT oil, see Table 1) was heated to a temperature of 100° C. in a reaction vessel placed in a hot water bath. 14.2 g of a solid fat particles F (Hydrogenated Soy Oil) were added to the heated oil phase, thereby melting the particles.
9.6 g of a 50:50 w/w an emulsifier blend of Dimodan HP and Citrem Liq K was added to this heated oil mixture phase O (continuous phase). All these steps were performed while stirring with an impeller stirrer at a slow stirring speed of 100 rpm.
An aqueous droplet phase A (Fatty Juicy Flavor (water soluble flavor)) was separately pre-heated to a temperature of 80° C., avoiding boiling. The stirring speed of the impeller stirrer was then increased to 3000 rpm and 33.4 g of the water phase A was added an approximate addition rate of 2 milliliters per second, thereby emulsifying the water droplets phase A into the heated oil phase O. This liquid pre-emulsion was left to stir for two minutes at the elevated stirring speed.
The temperature of the water bath was then reduced to 10° C. over the course of 30 minutes with the stirrer mixing at a slower speed of 200 rpm to obtain the final water-in-oil emulsion.
The emulsion obtained presented a thick creamy spreadable texture and remained stable against sedimentation of droplets and against phase separation.
The amounts of ingredients used for obtaining the water-in-oil emulsion are indicated in Table 1.
The emulsifiers (Citrem and Lecithin see table 2 hereunder) were mixed in an equal quantity of Sunflower oil (an edible oil) and heated to 65-70° C. to melt and dissolve. The remaining continuous Sunflower Oil and Cocoa Butter (solid fat particles F) are heated to 55° C. in a Thermomix at 100 rpm to melt and combine. The dissolved emulsifiers were then added to the bulk oils.
The two oil soluble flavors (Pork Fat Type Thermal Reaction Flavor from Firmenich and the Pork Sausage Type Liquid Flavor from Firmenich) were then added and dispersed in the oil continuous phase.
Setting the Thermomix to 300 rpm the water soluble flavor (Fatty Juicy Flavor aqueous phase) is gradually added to the oil phase in a continuous stream (approx. 100 g per minute) in order to achieve a fine dispersion and emulsification.
The emulsion is then transferred to a cooling vessel and rapidly cooled in an ice bath to 5-12 deg ° C. cooling at approximately 1.5° C. per minute with continuous stirring at 1500 rpm in order to achieve a cream like texture.
Pork Fat Type Thermal Reaction Flavor (Oil soluble flavor) (Firmenich) 0.2% as consumed.
To check the performance of the flavor delivering system of the invention through the extrusion process, the comparison between extruded and non-extruded flavored Pea Protein Isolate samples was done by measuring the total concentration of volatiles released in headspace above slurries using analytical technique.
The water-in-oil emulsion flavor containing the pork flavor (Pork Fat Type Thermal Reaction Flavor (Oil soluble flavor) Firmenich) (Example 2) was mixed with Pea Protein Isolate (PPI) (Nutralys F85M, from Roquette freres) at 1.5% dosage and extruded at different temperatures. The oil reaction flavor (Pork Fat Type Thermal Reaction Flavor (Oil soluble flavor) Firmenich) (Example 3) was mixed with Pea Protein Isolate (PPI) (Nutralys F85M) at 0.2% dosage and extruded at different temperatures.
All flavored systems were evaluated using AFFIRM (Analysis of Flavors and Fragrances In Real time) at iso-protein concentration load.
20 grams of each extruded system was blended with 80 grams of water for 1 minute and transferred in a 500 ml Schott bottle. 8 grams of non-extruded flavored PPI powder was mixed with 92 grams of water in a 500 ml Schott bottle.
The Schott bottles were sealed, stirred and allowed to equilibrate for 1 hour. The headspace was sampled for 1 minute. The signal intensity of all the molecules released in the headspace was determined by subtracting the background signal from the unflavored sample signal.
The total ion signal intensity of the unflavored non-extruded pea protein isolate and unflavored extruded pea protein isolate samples at iso-protein concentration is represented in
The total ion signal intensity of the flavored extruded water-in-oil emulsion pea protein isolate and flavored extruded oil reaction pea protein isolate samples is represented in
For the water-in-oil emulsion flavored sample (see
To confirm the emulsion type of the material as prepared in Example 1 and Example 2, dilution tests with heated hydrophobic vs. hydrophilic solvents were performed: a sample of 0.1 g of the material was mixed into 3 g of hot water (heated to 80° C.), and a second sample of 0.1 g of the material was mixed into equally heated isopropyl myristate (a hydrophobic solvent). Both samples were then observed by light microscopy.
In the sample mixed into heated isopropyl myristate, the heated emulsion blended and diluted well with the heated hydrophobic solvent, and dispersed emulsion droplets with an unambiguous size distribution could be observed by microscopy. In contrast, for the heated emulsion sample mixed into hot water, no blending and dilution was possible, and the material prepared according to the invention presented as an amorphous oily lump in the hot water.
This dilution tests confirmed that the emulsion type was water-in-oil; in a second part of the test, the droplet size distribution of the aqueous droplets diluted in heated isopropyl myristate was measured by image analysis using the software of the light microscope (Nikon Eclipse Software). The resulting drop size distribution is shown in
In particular, the elastic modulus (G′) and the viscous modulus (G″) were measured during two different types of tests:
In both kinds of tests, the relative magnitudes of the elastic (G′) and viscous (G″) moduli reveal the character of the material: G′>G″ means the material is predominantly elastic (‘solid-like’ and resistant to flow), whereas G″>G′ means the material is predominantly ‘fluid-like’. Likewise, the overall strength or softness of the material is reflected in the overall level of the moduli.
Temperature Sweep Experiments: Measurement of Viscoelastic Properties while Melting the Material Prepared According to the Invention
The temperature-dependent behaviour of the material as prepared in the previous examples is shown in
Surprisingly, however, even as the material prepared according to the invention melts, the elastic modulus remains superior to the viscous modulus, G′>G″. This behaviour is due to the presence of the dispersed phase in the material as formulated in Example 2 and it prevents the material from directly becoming purely liquid-like upon melting. This behaviour is in stark contrast to a classic, simple fat material, which simple becomes liquid upon melting.
This type of soft solid behaviour is advantageous for processing applications of the material, for example during extrusion processing, as it provides a barrier to flow for the material under applied stresses of the processing, thereby allowing to avoid excessive dispersion of the fat and consequently avoiding loss of structural integrity of the fat phase.
To further evaluate the soft-solid character of the material prepared according to the invention,
Table 3 below shows typical ingredients for an emulsion according to certain embodiments of the present disclosure. Amounts are given in percent by weight, based on the total weight of the emulsion.
Table 4 below shows typical ingredients for an artificial beef product that includes emulsions of Example 6. Amounts are given in percent by weight, based on the total weight of the emulsion.
A plant-based hamburger was made according to Example 7, and evaluated in comparison to a comparable hamburger in which the rapeseed oil, coconut oil, and flavouring emulsion were replaced with beef tallow. A group of eight (8) expert sensory panellists tasted the two hamburgers and evaluated their taste. The results of the sensory testing (as an average) are shown in Table 5.
For each of these measures, the differences were not statistically significant, suggesting that expert sensory testers could not distinguish significantly between a hamburger containing beef tallow versus one containing vegetable fats and a flavouring emulsion of the present disclosure.
The present application claims the benefit of priority of U.S. Provisional Application No. 63/129,700, filed Dec. 23, 2020, which is hereby incorporated by reference as though set forth herein in its entirety.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2021/086822 | 12/20/2021 | WO |
| Number | Date | Country | |
|---|---|---|---|
| 63129700 | Dec 2020 | US |
