Patent Application
20230374103
The present invention relates to a Wnt signaling pathway inhibitor and the like.
Scleroderma is a disease in which skin sclerosis progresses and visceral lesions appear within 5 to 6 years after onset, and the number of patients with scleroderma is more than 20,000 in Japan alone. Steroids, cyclophosphamide, proton pump inhibitors, prostacyclin, ACE inhibitors, endothelin receptor antagonists, and the like have been used as treatments for scleroderma; however, they do not lead to fundamental treatment.
Idiopathic pulmonary fibrosis (IPF) has an average survival period of 3 to 5 years after confirmation of diagnosis and an average survival period of less than 2 months after acute exacerbation. Prevention and treatment are important. Pirfenidone, nintedanib, and the like are marketed as IPF therapeutic drugs; however, they do not lead to fundamental treatment.
The number of cancer patients is increasing with the progress of aging and changes in lifestyle habits. In particular, lung cancer has a poor prognosis, and the development of effective therapeutic agents has been desired.
PTL 1 has reported that RANKL peptide inhibits inflammatory cytokines via TLR. However, it is not known that RANKL peptide is effective for prevention or treatment of fibrotic diseases such as scleroderma and pulmonary fibrosis, and cancer.
PTL 1: WO2018/084311
NPL 1: The Journal of Clinical Investigation, No. 7, Vol. 108, 2001, pages 971-979.
NPL 2: Mol Endocrinol, January 2009, 23(1): 35-46.
An object of the present invention is to provide a preventive or therapeutic agent for fibrotic diseases and cancer.
As a result of extensive research to achieve the above object, the present inventor found that an oligopeptide consisting of an amino acid sequence containing a DE loop sequence of RANKL protein and a β-strand D sequence of RANKL protein placed adjacent to the N-terminal side of the DE loop sequence can inhibit Wnt signaling pathway and suppress binding of LGR4to RSPO1-3. The present inventor also found that based on this action, the oligopeptide has a preventive or therapeutic effect on fibrotic diseases and cancer. As a result of further research based on this finding, the present inventor has completed the present invention. Specifically, the present invention includes the following embodiments.
Item 1. A Wnt signaling pathway inhibitor comprising an oligopeptide consisting of an amino acid sequence containing a DE loop sequence of RANKL protein and a β-strand D sequence of RANKL protein placed adjacent to the N-terminal side of the DE loop sequence,
wherein the DE loop sequence is the following amino acid sequence (a) or (b):
Item 2. The inhibitor according to Item 1, wherein the DE loop sequence is the amino acid sequence (a).
Item 3. The inhibitor according to Item 2, wherein the one to three amino acids are one or two amino acids.
Item 4. The inhibitor according to any one of Items 1 to 3, wherein in the amino acid sequence (d), the leucine residue at the N-terminus of SEQ ID NOs: 2 and 4, and the leucine residue that is the fourth amino acid residue from the N-terminus of SEQ ID NOs: 3 and 5, are not substituted or deleted.
Item 5. The inhibitor according to any one of Items 1 to 4, wherein the amino acid sequence of the oligopeptide contains a β-strand E sequence of RANKL protein placed adjacent to the C-terminal side of the DE loop sequence.
Item 6. The inhibitor according to Item 5, wherein the β-strand E sequence is the following amino acid sequence (e) or (f):
Item 7. The inhibitor according to any one of Items 1 to 6, wherein the amino acid sequence of the oligopetide is free from a CD loop sequence of RANKL protein.
Item 8. The inhibitor according to any one of Items 1 to 7, wherein the amino acid sequence of the oligopeptide is the following amino acid sequence (i) or (j):
Item 9. The inhibitor according to any one of Items 1 to 7, wherein the amino acid sequence of the oligopeptide is the following amino acid sequence (i′) or (j′):
(i′) the amino acid sequence represented by SEQ ID NO: 12, 17, 20, or 21, or
Item 10. The inhibitor according to any one of Items 1 to 9, wherein the oligopeptide has an acetylated N-terminus and an amidated C-terminus.
Item 11. The inhibitor according to any one of Items 1 to 10, wherein the oligopeptide has a length of 50 amino acid residues or less.
Item 12. The inhibitor according to Item 11, wherein the oligopeptide has a length of 40 amino acid residues or less.
Item 13. The inhibitor according to any one of Items 1 to 12, for use in prevention or treatment of a fibrotic disease, or for use in prevention or treatment of cancer.
Item 14. The inhibitor according to Item 13, wherein the fibrotic disease is a fibrotic disease in at least one member selected from the group consisting of skin and lung, and the cancer is lung cancer.
Item 15. A preventive or therapeutic agent for a fibrotic disease, the agent comprising an oligopeptide consisting of an amino acid sequence containing a DE loop sequence of RANKL protein and a β-strand D sequence of RANKL protein placed adjacent to the N-terminal side of the DE loop sequence,
wherein the DE loop sequence is the following amino acid sequence (a) or (b):
Item 16. A preventive or therapeutic agent for cancer, the agent comprising an oligopeptide consisting of an amino acid sequence containing a DE loop sequence of RANKL protein and a β-strand D sequence of RANKL protein placed adjacent to the N-terminal side of the DE loop sequence.
wherein the DE loop sequence is the following amino acid sequence (a) or (b):
Item 17. A method for inhibiting Wnt signaling pathway, the method comprising bringing an oligopeptide into contact with a cell, the oligopeptide consisting of an amino acid sequence containing a DE loop sequence of RANKL protein and a β-strand D sequence of RANKL protein placed adjacent to the N-terminal side of the DE loop sequence,
wherein the DE loop sequence is the following amino acid sequence (a) or (b):
(c) the amino acid sequence represented by any one of SEQ ID NOs: 2 to 5, or
Item 18. A method for preventing or treating a fibrotic disease or cancer, the method comprising administering an oligopeptide to a patient with a fibrotic disease or cancer, the oligopeptide consisting of an amino acid sequence containing a DE loop sequence of RANKL protein and a β-strand D sequence of RANKL protein placed adjacent to the N-terminal side of the DE loop sequence,
wherein the DE loop sequence is the following amino acid sequence (a) or (b):
Item 19. An oligopeptide for use as a Wnt signaling pathway inhibitor, the oligopeptide consisting of an amino acid sequence containing a DE loop sequence of RANKL protein and a β-strand D sequence of RANK protein placed adjacent to the N-terminal side of the DE loop sequence,
wherein the DE loop sequence is the following amino acid sequence (a) or (b):
Item 20. An oligopeptide for use as a preventive or therapeutic agent for a fibrotic disease or cancer, the oligopeptide consisting of an amino acid sequence containing a DE loop sequence or RANKL protein and a β-strand D sequence of RANKL protein placed adjacent to the N-terminal side of the DE loop sequence,
wherein the DE loop sequence is the following amino acid sequence (a) or (b):
Item 21. Use of an oligopeptide for producing a Wnt signaling pathway inhibitor, the, oligopeptide consisting of an amino acid sequence containing a DE loop sequence of RANKL protein and a β-strand D sequence of RANKL protein placed adjacent to the N-terminal side of the DE loop sequence,
wherein the DE loop sequence is the following amino acid sequence (a) or (b):
Item 22. Use of an oligopeptide for producing a preventive or therapeutic agent for a fibrotic disease or cancer, the oligopeptide consisting of an amino acid sequence containing a DE loop sequence of RANKL protein and a β-strand D sequence of RANKL protein placed adjacent to the N-terminal side of the DE loop sequence,
wherein the DE loop sequence is the following amino acid sequence (a) or (b):
The present invention can provide a Wnt signaling pathway inhibitor. The present invention can also provide a preventive or therapeutic agent for fibrotic diseases or cancer.
In the present specification, the amino acid sequences are all represented by single letters.
In the present specification, the terms “containing,” “comprising,” and “having” include: the concepts of “containing,” “including,” “consisting essentially of,” and “consisting only of.”
The “identity” of amino acid sequences refers to the degree of identicalness of amino acid sequences in two or more comparable amino acid sequences. Therefore, as the identity of two amino acid sequences is higher, the identity or similarity of the sequences is higher. The level of amino acid sequence identity is determined, for example, by FASTA, which is a tool for sequence analysis, using default parameters. Alternatively, the level of amino acid sequence identity can be determined using the algorithm BLAST by Karlin and Altschul (Karlin S, Altschul S F, “Methods for assessing the statistical significance of molecular sequence features by using general scoring schemes,” Proc Natl Acad Sci USA. 87: 2264-2288 (1990); and Karlin S, Altschul S F, “Applications and statistics for multiple high-scoring segments in molecular sequences,” Proc Natl Acad Sci USA. 90: 5873-7 (1993)). A program called “BLASTX,” which is based on this BLAST algorithm, has been developed. Specific procedures of these analysis methods are known, and reference may be made to the website (http://www.ncbi.nlm.nih.gov/) of the National Center of Biotechnology information (NCBI). The “identity” of base sequences is also defined accordingly.
In the present specification, “conservative substitution” means that an amino acid residue is substituted with another amino acid residue having a similar side chain. For example, conservative substitutions include substitutions with amino acid residues having basic side chains, such as lysine, arginine, and histidine. Other examples of conservative substitutions include substitutions with the following amino acid residues: amino acid residues with acidic side chains, such as asparagic acid and glutamic acid; amino acid residues with uncharged polar side chains, such as glycine, asparagine, glutamine, serine, threonine, tyrosine, and cysteine; amino acid residues with nonpolar side chains, such as alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, and tryptophan; amino acid residues with β-branched side chains, such as threonine, valine, and isoleucine; and amino acid residues with aromatic side chains, such as tyrosine, phenylalanine, tryptophan, and histidine.
The following describes an oligopeptide consisting of an amino acid sequence containing a DE loop sequence of RANKL protein and a β-strand D sequence of RANKL protein placed adjacent to the N-terminal side of the DE loop sequence (which may be referred to as “the oligopeptide of the present invention” in the present specification).
The DE loop sequence of RANKL protein may be the following amino acid sequence (a) or (b):
The substitution in the amino acid sequence (b) is preferably conservative substitution. Further, the addition in the amino acid sequence (b) is preferably addition at the N-terminus or C-terminus.
The β-strand D sequence of RANKL protein is not particularly limited, as long as it is, among the amino acid sequences of RANKL protein, an amino acid sequence that forms β-strand D. The organism species from which the β-strand D sequence is derived is not particularly limited. Examples include various mammals, such as humans, monkeys, mice, rats, dogs, cats, and rabbits. Among these, humans, monkeys, mice, rats, etc., are preferable; humans, mice, etc., are more preferable; and humans are even more preferable. β-strand P sequences of various organism species are known. For example, those of mice and rats are disclosed in NPL 1, NPL 2, etc. Even if a β-strand D sequence derived from a certain organism species is not known, the sequence can be easily determined based on known information (e.g., NPL 1 and NPL 2). Specific examples of the β-strand D sequence of RANKL protein include the amino acid sequence represented by SEQ ID NO: 2 (part of a β-strand D sequence of mouse-derived RANKL protein), the amino acid sequence represented by SEQ ID NO: 3 (a β-strand D sequence of mouse-derived RANKL protein), the amino acid sequence represented by SEQ ID NO: 4 (part of a β-strand D sequence of human-derived RANKL protein), the amino acid sequence represented by SEQ ID NO: 5 (a β-strand D sequence of human-derived RANKL protein), and the like. The β-strand D sequence may be mutated as long as the oligopeptide of the present invention can exhibit a preventive or therapeutic effect on fibrotic diseases or cancer, an inhibitory effect on Wnt signaling pathway, and the like.
In SEQ ID NOs: 2 to 5, the leucine residues at the N-terminus of SEQ ID NOs: 2 and 4, and the leucine residue that is the fourth amino acid from the N-terminus of SEQ ID NOs: 3 and 5, are important for exhibiting a preventive or therapeutic effect on fibrotic diseases or cancer, an inhibitory effect on Wnt signaling pathway, and the like. In the β-strand D sequence, it is preferable that these leucine residues be not mutated.
The β-strand D sequence of RANKL protein is preferably the following amino acid sequence (c) or (d):
In (d) above, the number of mutated (substituted, deleted, added, or inserted) amino acids is preferably 1 or 2, and more preferably 1.
Preferred mutations in (d) above include conservative substitution. The amino acid sequence (d) is preferably the following amino acid sequence (d′):
In the sequence (d′), the number of amino acids added is preferably 1 or 2 and more preferably 1.
The phrase “placed adjacent to the N-terminal side” indicates that the amino acid at the N-terminus of the DE loop sequence and the amino acid at the C-terminus of the above β-strand D sequence are linked by a peptide bond.
The amino acid sequence of the oligopeptide of the present invention may contain other sequences than those described above, as long as the oligopeptide of the present invention can exhibit a preventive or therapeutic effect on fibrotic diseases or cancer, an inhibitory effect on Wnt signaling pathway, and the like. The other sequences are not particularly limited, but are preferably determined from the viewpoint, for example, that the intracellular half-life is longer. The hydrophilicity of the oligopeptide and the intracellular half-life can be presumed based on various websites (e.g., ExPASy (http://web.expasy.org/protparam/)). When ExPASy is used regarding hydrophilicity, it is preferable to design a sequence so that the “grand average of hydropathicity” shows a negative value.
The other sequences particularly preferably include a β-strand E sequence of RANKL protein, from the viewpoint of a preventive or therapeutic effect on fibrotic diseases or cancer, an inhibitory effect on Wnt signaling pathway, and the like. The β-strand E sequence is preferably placed adjacent to the C-terminal side of the DE loop sequence. The phrase “adjacent to the C-terminal side” indicates that the amino acid at the C-terminus of the DE loop sequence and the amino acid at the N-terminus of the above β-strand E sequence are linked by a peptide bond.
The β-strand E sequence of RANKL protein is not particularly limited, as long as it is, among the amino acid sequences of RANKL protein, an amino acid sequence that forms β-strand E. The organism species from which the β-strand E sequence is derived is not particularly limited. Examples include various mammals, such as humans, monkeys, mice, rats, dogs, cats, and rabbits. Among these, humans, monkeys, mice, rats, etc., are preferable; humans, mice, etc., are more preferable; and humans are even more preferable. β-strand E sequences of various organism species are known. For example, those of mice and rats are disclosed in NPL 1, NPL 2, etc. Even if a β-strand E sequence derived from a certain organism species is not known, the sequence can be easily determined based on known information (e.g., NPL 1 and NPL 2). Specific examples of the β-strand E sequence of RANKL protein include the amino acid sequence represented by SEQ ID NO: 6(a β-strand E sequence of mouse-derived RANKL protein), the amino acid sequence represented by SEQ ID NO: 7 (part of a β-strand E sequence of mouse-derived RANKL protein), the amino acid sequence represented by SEQ ID NO: 8 (a β-strand E sequence of human-derived RANKL protein), the amino acid sequence represented by SEQ ID NO: 9 (part of a β-strand E sequence of human-derived RANKL protein), and the like. The β-strand E sequence may be mutated as long as the oligopeptide of the present invention can exhibit a preventive or therapeutic effect on fibrotic diseases or cancer, an inhibitory effect on Wnt signaling pathway, and the like.
The β-strand E sequence of RANKL protein is preferably the following amino acid sequence (e) or (f):
In (f) above, the number of mutated (substituted, deleted, added, or inserted) amino acids is preferably 1 or 2, and more preferably 1.
Preferred mutations in (f) above include conservative substitution. Further, the amino acid sequence of (f) above is preferably the following amino acid sequence (f′):
In (f′) above, the number of amino acids added is preferably 1 or 2, and more preferably 1.
The amino acid sequence of the oligopeptide of the present invention is preferably free from a CD-loop sequence of RANKL protein.
The CD loop sequence of RANKL protein is, among the amino acid sequences of RANKL protein, an amino acid sequence that forms a CD loop. CD loop sequences of various organism species are known. For example, those of mice and rats are disclosed in NPL 1, NPL 2, etc. Specific examples of the CD loop sequence of RANKL protein include the amino acid sequence represented by SEQ ID NO: 10 (a CD loop sequence of mouse-derived RANKL protein), the amino acid sequence represented by SEQ ID NO: 11 (a CD loop sequence of human-derived RANKL protein), and the like.
The length of the oligopeptide of the present invention is not particularly limited as long as it is a general length as an oligopeptide. The length is, for example, 50 amino acid residues or less, preferably 40 amino acid residues or less, more preferably 35 amino acid residues or less, still more preferably 30 amino acid residues or less, and still more preferably 25 amino acid residues or less. The length is, for example, 11 amino acid residues or more, preferably 13 amino acid residues or more, more preferably 15 amino acid residues or more, still more preferably 20 amino acid residues or more, and still more preferably 25 amino acid residues or more. The length is preferably, for example, 11 to 50 amino acid residues, more preferably 15 to 40 amino acid residues, still more preferably 20 to 35 amino acid residues, and still more preferably 25 to 30 amine acid residues.
The amino acid sequence of the oligopeptide of the present invention is preferably the following amino acid sequence (i) or (j):
The amino acid sequence of the oligopeptide of the present invention is more preferably the following amino acid sequence (i′) or (j′):
In (j) and (j′) above, the number of mutated (substituted, deleted, added, or inserted) amino acids is preferably 1 or 2, and more preferably 1.
The substitution in the amino acid sequences (j) and (j′) is preferably conservative substitution.
The oligopeptide of the present invention may be chemically modified as long as it can exhibit a preventive or therapeutic effect on fibrotic diseases or cancer, an inhibitory effect on Wnt signaling pathway, and the like.
The C-terminus of the oligopeptide of the present invention may be a carboxyl group (—COOH), carboxylate (—COO−), amide (—CONH2), or ester (—COOR).
Examples of R in the ester include C1-6 alkyl groups, such as methyl, ethyl, n-propyl, isopropyl, and n-butyl; C3-8 cycloalkyl groups, such as cyclopentyl and cyclohexyl; C6-12 aryl groups, such as phenyl and α-naphthyl; phenyl-C1-2 alkyl groups, such as benzyl and phenethyl; C7-14 aralkyl groups, such as α-naphthyl-C1-2 alkyl groups (e.g., α-naphthylmethyl); pivaloyloxymethyl groups; and the like.
Furthermore, the oligopeptide of the present invention also includes oligopeptides in which the amino group of the N-terminal amino acid residue is protected by a protecting group (e.g., C1-6 acyl groups such as C1-6 alkanoyl, including formyl and acetyl groups), etc.
The chemical modification is preferably amidation at the C-terminus, protection at the N-terminus with an acetyl group, and the like. In particular, an oligopeptide with an acetylated N-terminus is preferable for its excellent activity. In terms of stability, an oligopeptide with an amidated C-terminus is excellent. Examples of more preferred oligopeptides include oligopeptides with an amidated C-terminus and an acetylated N-terminus. Examples of particularly preferred oligopeptides include MHP1-7 obtained by amidating the C-terminus of MHP1 and acetylating the N-terminus of MHP1, MHP6-AcN obtained by amidating the C-terminus of MHP6 and acetylating the N-terminus of MHP6, MHP24-AcN obtained by amidating the C-terminus of MHP24 and acetylating the N-terminus of MHP24, and MHP24h-AcN obtained by amidating the C-terminus of MHP24h and acetylating the N-terminus of MHP24h.
A known substance for modification may be further added to the oligopeptide of the present invention, for the purpose of improving the stability, pharmacokinetics, bioavailability, etc., of drugs. Such substances for modification include, for example, polyethylene glycol chains.
The oligopeptide of the present invention includes those having various forms, such as linear oligopeptides, branched oligopeptides, and cyclic oligopeptides, with linear oligopeptides being preferable. The oligopeptide of the present invention may be crosslinked by or based on a known means as long as can exhibit a preventive or therapeutic effect on fibrotic diseases or cancer, an inhibitory effect on Wnt signaling pathway, and the like.
The oligopeptide of the present. invention may be in the form of a pharmaceutically acceptable salt with an acid or base. The salt is not particularly limited, as long as it a pharmaceutically acceptable salt. Acid salts and basic salts can both be employed. Examples of acid salts include inorganic acid salts, such as hydrochloride hydrobromate, sulfate, nitrate, and phosphate; organic acid salts, such as acetate, propionate, tartrate, fumarate, maleate, malate, citrate, methanesulfonate, and p-toluenesulfonate; amino acid salts, such as aspartate and glutamate; and the like. Examples of basic salts include alkali metal salts, such as sodium salt and potassium salt; alkaline earth metal salts, such as calcium salt and magnesium salt; and the like.
The oligopeptide of the present invention may be in the form of a solvate. The solvent is not particularly limited, as long as it is pharmaceutically acceptable. Examples include water, ethanol, glycerol, acetic acid, and the like.
The oligopeptide of the present invention can be produced by a known peptide synthesis method, depending on its amino acid sequence.
Since the oligopeptide of the present invention has a preventive or therapeutic effect on fibrotic diseases or cancer, an inhibitory effect on Wnt signaling pathway, an inhibitory effect on binding of LGR4 to RSPO (e.g., RSPO1, RSPO2, and RSPO3), and an inhibitory effect on epithelial-mesenchymal transition (EMT), it can be used as an active ingredient for preventive or therapeutic agents for fibrotic diseases or cancer, Wnt signaling pathway inhibitors, binding inhibitors of LGR4 and RSPO1, epithelial-mesenchymal transition inhibitors, and the like.
In the present specification, the above medical agents are also collectively referred to as “the agent of the present invention.” The agent of the present invention can be used in various fields, such as medicine. The agent of the present invention may be applied to (e.g., administered to, ingested by, inoculated into) animals and humans directly or as various compositions in combination with conventional components.
In the agent of the present invention, the oligopeptides of the present invention may be used alone or in a combination of two or more.
Fibrotic diseases are diseases in which part of the biological tissue is fibrosis. Fibrotic diseases are not particularly limited, as long as they are diseases associated with tissue fibrotic conditions or tissue fibrosis. Examples of fibrotic tissues include lungs, skin, kidneys, heart, liver, bladder, gastrointestinal tract, blood vessels, and the like; and preferably skin, lungs, and the like. Examples of fibrotic diseases include scleroderma, pulmonary fibrosis, idiopathic pulmonary fibrosis, interstitial pulmonary fibrosis, renal fibrosis, renal interstitial fibrosis, liver fibrosis, peritoneal fibrosis, myocardial fibrosis, skin fibrosis, skeletal muscle fibrosis, pancreatic fibrosis, neurofibroma, vascular fibrosis, and the like. Preferred among these are scleroderma, pulmonary fibrosis, and the like.
Examples of cancer include, but are not particularly limited to, lung cancer, gastric cancer, liver cancer, esophageal cancer, pancreatic cancer, colon cancer, biliary tract cancer, kidney cancer, bladder cancer, uterine cancer, ovarian cancer, breast cancer, prostate cancer, testicular cancer, skin cancer, leukemia, bone tumor, osteosarcoma, soft tissue tumor, multiple myeloma, malignant lymphoma, pharyngeal cancer, head and neck cancer, childhood cancer, and the like. Preferred among these is lung cancer. In one embodiment of the present invention, cancer with high expression of LGR4 and RSPO (e.g., RSPO1, RSPO2, and RSPO3) is preferred.
Wnt signaling pathway inhibitors suppress or inhibit Wnt signaling. Several Wnt signaling pathways are known, but are not particularly limited. Preferred are suppressors or inhibitors of Wnt signaling pathway through β-catenin. More preferred are suppressors or inhibitors of Wnt signal activation by RSPO (R-spondin).
The content of the active ingredient in the agent of the present invention can be suitably determined taking into consideration the type of target disease, the intended therapeutic effect, the administration method, the treatment period, the patient's age, the patient's body weight, etc. For example, the content of the active ingredient in the agent of the present invention can be about 0.0001 parts by weight to 100 parts by weight based on the entire agent of the present invention.
The administration form of the agent of the present invention is not particularly limited as long as a desired effect is exhibited. The agent can be administered to mammals, including humans through an administration route, either peroral or parenteral administration, (e.g., intravenous injection, intramuscular injection, subcutaneous administration, rectal administration, transdermal administration, and local administration). The administration form is preferably parenteral administration. The dosage form and production method of the agent for peroral or parenteral administration are well known to those skilled in the art, and the agent in any dosage form can be produced in accordance with conventional methods, for example, by mixing the active ingredient with a pharmaceutically acceptable carrier and the like.
The dosage form for parenteral administration includes injectable drugs (e.g., drip-injectable drugs, intravenously injectable drugs, intramuscularly injectable drugs, subcutaneously injectable drugs, and intradermally injectable drugs), drugs for external use (e.g., ointments, cataplasms, lotions, creams, and gels), suppositories, inhalants, eye drops, eye ointments, nasal drops, ear drops, liposome drugs, and the like. For example, an injectable drug can be prepared by dissolving the oligopeptide of the present invention in injectable distilled water; and a solubilizing agent, a buffer, a pH adjuster, a toxicity agent, a soothing agent, a preservative, a stabilizer, and the like can be optionally added thereto. The agent of the present invention may in the form of a freeze-dried formulation that is prepared when needed.
The agent of the present invention may further comprise other medical agents effective in treatment or prevention of diseases. The agent of the present invention may also optionally contain components such as a sterilizer, an antiphlogistic, a cellular stimulant, vitamins, and an amino acid.
For the carriers used in formulating the agent of the present invention, an excipient, a binder, a disintegrant, a lubricant, a colorant, and a flavoring agent typically used in this technical field can be used; and a stabilizer, an emulsifier, an absorption promoter, a surfactant, a pH adjuster, an antiseptic, an antioxidant, a filler, a moisture agent, a surface activation agent, a dispersant, a buffer, a preservative, a solubilizing agent, a soothing agent, and the like can also optionally be used.
The dose of the agent of the present invention can be determined taking into consideration various factors, such as the administration route, the type of disease, the degree of symptoms, the patient's age, gender, body weight, severity of disease, pharmacological findings such as pharmacokinetics and toxicological characteristics, use or non-use of a drug delivery system, and whether the agent is administered as part of a combinational drug with other drugs. For example, the dose of the agent of the present invention can be about 1 μg/kg (body weight) to 10 g/kg (body weight) per day. The administration schedule of the agent of the present invention can also be determined in consideration of the same factors as the dose. For example, the dose per day as described above can be administered 1 to 5 times a day to a month.
The present invention is described in detail below based on Examples; however, the present invention is not limited to these Examples.
Synthesis of oligopeptides consisting or the amino acid sequences shown in Table 1 was entrusted to ILS Inc. Those other than MHP24-AcN and MHP24h-AcN have no modification at the N-terminus and C-terminus, and the N-terminus is an amino group while the C-terminus is a carboxyl group. MHP24-AcN and MHP24h-AcN are oligopeptides with an acetylated N-terminus and an amidated C-terminus. Amino acid residues in these peptides are all L-forms.
Further, synthesis of an oligopeptide obtained by replacing the methionine residue of MHP1 with a D-form (MHP1-3), an oligopeptide obtained by acetylating the N-terminus of MHP1 (MHP1-6), an oligopeptide obtained by acetylating the N-terminus of MHP1 and amidating the C-terminus of MHP1 (MHP1-7 or MHP1-AcN), and an oligopeptide obtained by amidating the C-terminus of MHP1 (MHP1-8) was entrusted to ILS Inc.
Further, synthesis of an oligopeptide obtained by acetylating the N-terminus of MHP1 and adding a biositin (biotinylated lysine) residue to the C-terminus (biotinylated MHP1) was entrusted to Bachem.
HPLC and MS confirmed that oligopeptides with the desired sequences were synthesized with high purity. Hereinafter, these peptides are also generically referred to as “the RANKL peptide.”
The influence of RANKL peptide on Wnt signaling was analyzed. Specifically, the following procedure was performed.
HEK293 cells expressing luciferase under the control of β-catenin responsive element were plated on a 96 -well plastic plate (1×104 cells/well) and incubated in the presence or absence of 50 ng/mL of recombinant mouse wnt3a (R&D System, #1324-WN) and recombinant human RSPO1 (R&D System, #4645-RS), recombinant human RSPO2 (PeproTech, #120-43), or recombinant human RSPO3 (PeproTech, #120-44). When these were added at the same time as RANKL peptide, 50 ng/mL of recombinant mouse wnt3a and 20 ng/mL of RSPO1/2/3 were used. After incubation for 24 hours, luciferase activity was analyzed using a luciferase assay system (Promega) and a microplate luminometer (Centro XS3 LB960; Berthold Technologies). The protein concentration of each sample was measured using Takara Bradford Protein Assay Kit (Takara Bio Inc.). The results of luciferase activity were normalized to the protein concentration of each sample.
The results are shown in
Regarding binding of RANKL peptide to LGR4, the influence of RANKL peptide on binding of LGR4 to its ligand, RSPO1, was analyzed. Specifically, the following procedure was performed.
HEK293 cells were incubated with 100 μg/mL of RANKL peptide or biotinylated RANKL peptide for 30 minutes, followed by immunoprecipitation. For competitive binding analysis, HEK293 cells were transfected with a Flag-tagged LGR4 expression plasmid (OriGene Technologies) for 48 hours, and incubated for 2 hours in the presence of 50 ng/mL L of recombinant human biotinylated RSPO1 (R&D System, #BT4645) and RANKL peptide. For immunoprecipitation, the cells were washed twice with cold PBS and dissolved in a lysis buffer (50 mM Tris-HCL, pH 7.4, 150 mM NaCl, 5 mM EDTA, 0.5% NP40, 10% glycerol and protease inhibitor cocktail (Roche)). The sample was centrifuged at 16,000 g for 10 minutes. The supernatant was collected, and the protein concentration was determined. Immunoprecipitation from samples containing equal amounts of protein was performed at 4° C. overnight using high-capacity streptavidin agarose (Thermo Fisher Scientific or Anti-FLAG M2 Affinity Gel (Sigma-Aldrich), followed by washing twice with a lysis buffer, and elution with 2× Laemmli Sample Buffer at 37° C. for 30 minutes before Western blotting. The blotted membrane was incubated with the primary antibody at 4° C. overnight, washed with TBS containing 0.1% Tween-20, then incubated with the HRP-conjugated secondary antibody at room temperature for 1 hour, and then incubated with Chemi-Lumi One L (Nacalai Tesque). The antibodies and reagents are as follows: anti-LGR4 (Thermo Fisher Scientific, PA5-67868), anti-GAPDH (Sigma-Aldrich, MAB374), anti-FLAG (Sigma-Aldrich, F1804), anti-mouse IgG-HRP (NA931V, GE Healthcare), anti-rabbit IgG-HRP (NA934V, GE Healthcare), and HRP-Streptavidin (Sigma-Aldrich).
The results are shown in
A bleomycin-induced scleroderma model was produced, and whether the symptoms were improved by the early administration of RANKL peptide was examined. Specifically, the following procedure was performed.
As test animals, C57BL6/J mice (female, 8 weeks old) were prepared. 200 μg/mouse of bleomycin was subcutaneously injected at a frequency of 5 days/week for 14 days, and in parallel with this, 800 μg/mouse of saline or RANKL peptide was intraperitoneally administered at a frequency of 5 days/week for 14 days. The body weight was measured periodically during the dosing period. The mice were sacrificed 14 days after the start of administration and the skin tissue of the bleomycin-administered portion was stained with Masson's trichrome to evaluate fibrosis. Further, the expression of collagen gene mRNA, which is a fibrosis index, in the skin was evaluated.
The results are shown in
A bleomycin-induced scleroderma model was produced, and whether the symptoms were improved by the late administration of RANKL peptide was examined. Specifically, the following procedure was performed.
As test animals, C57BL6/J mice (female, 8 weeks were prepared. 200 μg/mouse of bleomycin was subcutaneously injected at a frequency of 5 days/week for 14 days. Subsequently, 800 μg/mouse of saline or RANKL peptide was intraperitoneally administered at a frequency of 5 days/week for 14 days. The nice were sacrificed after 14 days (28 days after the start of bleomycin administration), and the skin tissue of the bleomycin-administered portion was stained with Masson's trichrome to evaluate fibrosis. Further, the expression of collagen gene mRNA, which is a fibrosis index, in the skin was evaluated.
The results are shown in
A bleomycin-induced pulmonary fibrosis model was produced, and whether the symptoms were improved by the administration of RANKL peptide was examined. Specifically, the following procedure was performed.
As test animals, C57BL6/J mice (female, 8 weeks old) were prepared. Saline or RANKL peptide (800 μg/mouse) was administered intraperitoneally, and immediately thereafter, saline or bleomycin (0.05 μg/mouse) was directly administered intratracheally. The mice were sacrificed 14 days after administration, the lung weight and body weight were measured, and the lung weight/body weight ratio was determined. In addition, the lung tissue, was stained with Masson's trichrome, and Masson's trichrome score evaluation (0: normal, 1: fibrotic lesions predominantly at the margin of the lung, 2: marginal or global multifocal fibrotic lesions, 3: fibrotic lesions in approximately ⅓ of the area, 4: fibrotic lesions accounting for ⅔ or more of the area) was performed based on the stained images. Further, the expression of fibrosis marker gene mRNA in lung tissue was evaluated.
The results are shown in
The influence of RANKL peptide on cancer was analyzed.
The influence RANKL peptide on the proliferation of A549 cells (human lung alveolar basal epithelial cells: high expression of both LGR4 and RSPO3 was confirmed) was examined. Specifically, the following procedure was performed. MHP1 was administered at 1 ng/ml to 100 μg/ml to A549 cells, and the cell viability was calculated by MTT assay after 40 hours.
The results are shown in
The influence of RANKL peptide on the migration and invasion of A549 cells was examined. Specifically, the following procedure was performed. A chamber of an 8-μm polycarbonate membrane filter (Neuro Probe) was installed in the upper compartment of a Boyden chamber (48-well Chamber, Neuro Probe), A549 cells were seeded in serum-free DMEM containing MHP1-AcN, and DMEM containing 10% FBS was placed in the lower compartment, followed by incubation at 37° C. for 15 hours. In the invasion assay, the upper compartment was Matrigel Invasion chamber (Corning Life Science). Similar A549 cells were seeded in serum-free medium containing MHP1-AcN, and DEM containing 10% FBS was placed in the lower compartment. Cells adhering to the upper surface of each upper compartment were detached, the lower surface was stained with Diff-Quick (Sysmex), and the number of cells was counted under a microscope.
The results are shown in
The influence of RANKL peptide on cancer in an A549 cell xenograft model was examined. Specifically, the following procedure was performed. 2×106 cells were administered subcutaneously to BALB/cAJcl-nu/nu cells. After one day, 600 μg/mouse of MHP1 was administered every day, and the tumor size was measured up to 49 days. Further, the tumor weight was measured.
The results are shown in
The influence of RANKL peptide on the EMT mechanism was analyzed.
The influence of RANEL peptide on smad2/3 activation induced by TGF-β was examined. Specifically, the following procedure was performed. After pretreatment of MRC-5 cells with MHP1 for 24 hours, 5 ng/ml of TGFβ was added to the medium, the medium was collected after 10 minutes, and WB was performed.
The results are shown in
The influence of RANKL peptide on the expression of TGF-β receptor was examined. Specifically, the following procedure was performed. MHP1 was added to the medium of MRC-5 cells, the cells were collected after 24 hours, and the expression of TβR1 and TβR2 was analyze by real-time RT-PCR.
The results are shown in
The influence of RANKL peptide on the expression of α-SMA and Colla1 induced by TGF-β was examined. Specifically, the following procedure was performed. After pretreatment of MHP1 with MRC-5 cells for 24 hours, 2 ng/m/ of TGFβ was added, the cells were collected after 24 hours, and mRNA levels were determined by real-time RT-PCR.
The results are shown in
Sequence Listing
| Number | Date | Country | Kind |
|---|---|---|---|
| 2020-168029 | Oct 2020 | JP | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/JP2021/035368 | 9/27/2021 | WO |
