Claims
- 1. An AAV-based vector that is suitable for transferring a heterologous DNA fragment into cells in vitro, comprising:
- (a) vector pWC3,
- (b) a heterologous promoter oriented so as to promote transcription towards the AAV polyadenylation signal of said pWC3 vector, and
- (c) a heterologous DNA fragment, wherein said heterologous DNA fragment is under the regulatory control of said heterologous promoter.
- 2. An AAV-based vector according to claim 1, wherein said heterologous DNA fragment encodes an antisense polynucleotide sequence that is complementary to a targeted RNA sequence.
- 3. An AAV-based vector according to claim 1, wherein said heterologous DNA fragment is a gene that encodes a protein of interest.
- 4. An AAV-based vector according to claim 2, further comprising:
- (d) a second heterologous promoter oriented so as to promote transcription in a direction opposite to said heterologous promoter of (b), and
- (e) a second heterologous DNA fragment, wherein said second heterologous DNA fragment is either a gene that encodes a selectable marker or is a gene that encodes an enzymatically measurable marker protein, and wherein said second heterologous DNA fragment is under the regulatory control of said second heterologous promoter.
- 5. An AAV-based vector according to claim 4, wherein said targeted RNA sequence is ICP4 of herpes simplex virus.
- 6. An AAV-based vector according to claim 4, wherein said targeted RNA sequence is E1a of adenovirus.
- 7. An AAV-based vector according to claim 4, wherein said targeted RNA sequence is HIV-1 TAR.
- 8. An AAV-based vector according to claim 7, wherein said vector is pCWRSV-HIVAlpha-Neo.
- 9. An AAV-based vector according to claim 1, wherein said heterologous promoter is the Rous sarcoma virus promoter.
- 10. An AAV-based vector which is pWC3.
- 11. An AAV-based vector which is pCWRSV.
Parent Case Info
This application is a continuation of application Ser. No. 07/752,899, filed Aug. 26, 1991, now abandoned, which is a continuation of U.S. Ser. No. 07/527,195, filed May 23, 1990, now abandoned.
US Referenced Citations (4)
Non-Patent Literature Citations (10)
Entry |
F. L. Moolten (1987) Medical Hypotheses 24:43-51. |
E. Gilboa (1988) Adv. Exp. Med. Biol: Mol. Biol. Hemopoiesis 24:29-33. |
D. Baltimore (1988) Nature 335:395-396. |
E. C. M. Mariman (1985) Nature 318:414. |
J. S. Lebkowski et al Mol. Cell. Biol. 8, 3988-3996 (1988). |
D. LaFace et al Virology. 162, 483-486 (1988). |
J. C. Sanford (1988) J. Theor. Biol. 130:469-480. |
F. Wong-Staal (1989) Serono Symposia 59:1-8. |
S. Chatterjee et al (1992) Science 258:1485-1488. |
M. I. Johnston et al. (1993) Science 260:1286-1293. |
Continuations (2)
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Number |
Date |
Country |
Parent |
752899 |
Aug 1991 |
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Parent |
527195 |
May 1990 |
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