Patent Application
20230374070
The present invention relates to an amyloid-β aggregation inhibitor, a pharmaceutical composition for an amyloid-β aggregation disease, and a use application of the same.
Alzheimer's disease, which predominantly occurs in old age, has become a serious issue due to the increasing proportion of the elderly in the population as a result of rising life expectancy. Alzheimer's is a progressive central neurodegenerative disease that results in cognitive dysfunction and memory loss. This disease is thought to be caused by fibrillar aggregates (amyloid fibrils) generated by aggregation of amyloid-β through intermolecular association in the brain. However, no clinically effective drugs have been implemented into practice, and further investigation of candidate drugs is required. This issue is not limited to Alzheimer's, but applies to all diseases caused by amyloid fibers.
Technical Problem
It is an object of the present invention to provide a novel drug that inhibits aggregation of amyloid-β, which causes Alzheimer's and the like.
The present invention is directed to an amyloid-β aggregation inhibitor characterized by containing at least one of a peptide having an amino acid sequence of Sequence ID No. 1 and a peptide having an amino acid sequence of Sequence ID No. 2.
The present invention is directed to a pharmaceutical composition for an amyloid-β aggregation disease, characterized by containing at least one of a peptide having an amino acid sequence of Sequence ID No. 1 and a peptide having an amino acid sequence of Sequence ID No. 2.
The present invention is directed to a method for inhibiting aggregation of amyloid-β, characterized by including adding the amyloid-β aggregation inhibitor of the present invention to a test subject.
The present invention is directed to a method for treating an amyloid-β aggregation disease, characterized by including administering the amyloid-β aggregation inhibitor of the present invention to a test subject.
The amyloid-β aggregation inhibitor of the present invention can inhibit the aggregation of amyloid-β through intermolecular association, thus enabling treatment, e.g., prevention, inhibition of progression, and amelioration, of amyloid aggregation diseases such as Alzheimer's.
<Amyloid-β Aggregation Inhibitor>
As described above, the amyloid-β aggregation inhibitor of the present invention is characterized by containing at least one of a peptide having an amino acid sequence of Sequence ID No. 1 and a peptide having an amino acid sequence of Sequence ID No. 2. The peptide of Sequence ID No. 1 is hereinafter also referred to as Peptide 1 or GSGNR, and the peptide of Sequence ID No. 2 is hereinafter also referred to as Peptide 2 or GSGFK.
Peptide 1 and Peptide 2 have constituent units that are amino acid residues that may be, for example, only in the D-form, only in the L-form, or in both the D-form and the L-form.
Peptide 1 and Peptide 2 may be, for example, chemically modified peptide isosters. The chemical modification may be performed, for example, on all of the amino acid residues or some of the amino acid residues. There is no particular limitation on the type of chemical modification, and examples thereof include substitution of hydrogen atoms with halogen atoms, substitution with non-naturally occurring amino acids corresponding to naturally occurring amino acids, introduction of methyl groups, modification of amino acid side chains, cyclization of peptides, modification of carboxyl groups and/or amino groups, and amidation of terminal carboxyl groups.
Peptide 1 and Peptide 2 can inhibit the aggregation of amyloid-β (hereinafter also referred to as AB) or fragment peptides thereof. That is to say, it is possible to inhibit the aggregation itself of amyloid-β (Aβ) or fragment peptides thereof through intermolecular association, and thus this effect can be said to be, for example, inhibition of the formation of aggregates. There is no particular limitation on the source of amyloid-β whose aggregation is inhibited by Peptide 1 and Peptide 2, and examples thereof include a human and a non-human animal, with the source preferably being a human. The length of the full-length amino acid sequence of human amyloid-β is, for example, the length corresponding to 40 to 42 amino acid residues. The length of the full-length amino acid sequence of human amyloid-β depends, for example, on the site of enzymatic cleavage from the amyloid precursor (APP). The human amyloid-β constituted by 42 amino acid residues is registered in the database (PubChem) under accession number CID: 57339251 and is represented by, for example, Sequence ID No. 3. The human amyloid-β constituted by 40 amino acid residues is a sequence from the 1-st amino acid residue (D) at the N-terminus to the 40-th amino acid residue (V) in Sequence ID No. 3, and the 40-th amino acid residue is the C-terminus.
Sequence ID No. 3: DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
The aggregation inhibitor of the present invention can be used to treat diseases (amyloid-β aggregation diseases) that are caused by, for example, aggregation of amyloid-β (Aβ) or fragment peptides thereof. In the present invention, treatment includes the meaning of prevention, inhibition of progression, and amelioration (alleviation), for example, and the aggregation inhibitor is particularly useful for prevention and inhibition of progression because it can inhibit the formation of aggregates itself. Prevention includes, for example, the meaning of prevention of recurrence. There is no particular limitation on the amyloid-β aggregation diseases, and examples thereof include diseases that can be caused by the aggregation of amyloid-β, and specifically include memory impairment, Alzheimer's, and cerebral amyloid angiopathy.
The aggregation inhibitor of the present invention is also referred to as an agglutination inhibiting composition of the present invention. The aggregation inhibitor of the present invention contains at least one of Peptide 1 and Peptide 2 as an active component. The active component contained in the aggregation inhibitor of the present invention may be Peptide 1 alone, Peptide 2 alone, or both Peptide 1 and Peptide 2. The aggregation inhibitor of the present invention may be, for example, a composition containing only the active component or a composition containing the active component and other additive components. There is no particular limitation on the additive components, and examples thereof include pharmacologically acceptable components. As for the additive components, reference can be made, for example, to the following descriptions regarding the pharmaceutical composition.
The aggregation inhibitor of the present invention can be used, for example, in environments in which amyloid-β (Aβ) or fragment peptides thereof are present or are presumed to be present. The aggregation inhibitor of the present invention, for example, can be added to a test subject. The test subject may be a non-living-being-derived test subject that does not contain cells and the like, or a living-being-derived test subject that is cells such as brain cells, tissue such as brain tissue, or an organism. In the case of the latter test subject, the addition of the aggregation inhibitor can be, for example, performed in vivo or in vitro. The cells and tissue may be sourced from a human or a non-human animal, for example, and the organism may be, for example, a human or a non-human animal. Examples of the non-human animal include mammalian animals such as mice, rats, rabbits, horses, sheep, cattle, and camels.
In the present invention, aggregation inhibition is, for example, inhibition (reducing) of amyloid-β aggregation, or inhibition (reducing) through dissociation of amyloid-β aggregates that have already been formed. Peptide 1 (GSGNR) of the present invention may be, for example, capable of dissociating amyloid-β aggregates and inhibiting aggregation itself. Peptide 2 (GSGFK) of the present invention may be, for example, capable of inhibiting amyloid-β aggregation itself and dissociating amyloid-β aggregates. The dissociation of amyloid-β aggregates means breaking the aggregates up into simple components, i.e., amyloid-β, for example, and excludes the meaning of degradation through cleavage in amyloid-β molecules, for example, in the amyloid-β aggregates (cleavage of amyloid-β by hydrolysis). In this case, the aggregation inhibitor of the present invention can be, for example, said to be an aggregation dissociating agent. The inhibition of amyloid-β aggregation itself is particularly preferable for prevention, inhibition of progression, amelioration, and the like, for example, and the dissociation of amyloid-β aggregates is particularly preferable, for example, for inhibition of progression, amelioration, and the like.
The aggregation inhibitor of the present invention may contain, for example, an additional peptide in addition to Peptide 1 and/or Peptide 2. The additional peptide may be, for example, in a form that is bonded to Peptide 1 or Peptide 2. The additional peptide may have, for example, a DDS ability to the site in which amyloid-β or amyloid-β aggregates are present. Furthermore, the additional peptide may be, for example, a signal peptide that is bonded to Peptide 1 or Peptide 2 at the time of administration to an organism and is cleaved from Peptide 1 or Peptide 2 by an enzyme or the like in the organism after administration to the organism.
The aggregation inhibitor of the present invention can be used, for example, also as a later-described pharmaceutical composition for an amyloid-β aggregation disease of the present invention. Furthermore, the aggregation inhibitor of the present invention can be used, for example, in a later-described method for inhibiting amyloid-β aggregation of the present invention and method for treating an amyloid-β aggregation disease. As for the aggregation inhibitor of the present invention, reference can be made to the following descriptions regarding the pharmaceutical composition for an amyloid-β aggregation disease, the method for inhibiting aggregation of amyloid-β, and the method for treating an amyloid-β aggregation disease of the present invention.
<Pharmaceutical Composition for Amyloid-β Aggregation Disease>
As described above, the pharmaceutical composition for an amyloid-β aggregation disease of the present invention (hereinafter also referred to as a pharmaceutical composition) is characterized by containing at least one of Peptide 1 having an amino acid sequence of Sequence ID No. 1 and Peptide 2 having an amino acid sequence of Sequence ID No. 2.
The pharmaceutical composition of the present invention is characterized by containing at least one of Peptide 1 and Peptide 2 that can inhibit the aggregation of amyloid-β or fragment peptides thereof, and there is no particular limitation on the other aspects of the configuration. As for Peptide 1 and Peptide 2, reference can be made to the foregoing descriptions regarding the aggregation inhibitor of the present invention.
The pharmaceutical composition of the present invention contains at least one of Peptide 1 and Peptide 2 as an active component. The active component contained in the pharmaceutical composition of the present invention may be Peptide 1 alone, Peptide 2 alone, or both Peptide 1 and Peptide 2, and may further include other active components on the amyloid-β aggregation diseases. The other active components may be an active component that inhibits the formation of aggregates or an active component that degrades aggregates that have been formed as with Peptide 1 and Peptide 2, for example, the latter being preferred. The degradation of aggregates may be, for example, degradation through cleavage of the aggregates using hydrolytic activity or the like, or degradation through dissociation of the aggregates into constituent molecules thereof (amyloid-β or fragment peptides thereof). Examples of the active component that degrades the aggregates through cleavage include a catalytic peptide that exhibits hydrolytic activity as disclosed in WO 2017/119511A, which is herein incorporated by reference. The pharmaceutical composition of the present invention particularly preferably contains an active component that inhibits the formation of aggregates itself (Peptide 1 and/or Peptide 2) and an active component that degrades the aggregates, for example, because the former inhibits the formation of aggregates itself, and, even if aggregates are formed, the latter can degrade aggregates that have been formed, and thus it is possible to provide double defense.
The pharmaceutical composition of the present invention may be, for example, a composition containing only the active component or a composition containing the active component and other additive components. There is no particular limitation on the additive components, and examples thereof include pharmacologically acceptable components. The additive components may be, for example, set as appropriate according to the administration method, the administration site, the dosage form, and the like of the pharmaceutical composition of the present invention.
There is no particular limitation on the administration method of the pharmaceutical composition of the present invention, and examples thereof include parenteral administration and oral administration. Parenteral administration may be, for example, intravenous administration.
Examples of parenteral administration include administration by injection at an affected site, intravenous injection, subcutaneous injection, intradermal injection, and intravenous drip injection, nasal administration, and dermal administration. In the case of parenteral administration, there is no particular limitation on the administration site, and the pharmaceutical composition may be, for example, directly administered to a treatment site or indirectly administered to a treatment site. In the latter case, the administration site, for example, is a site from which the active component of the pharmaceutical composition of the present invention can be delivered to the treatment site. In many cases, amyloid-β aggregation diseases are caused by, for example, aggregation of amyloid-β in the brain, as in the case of memory impairment and Alzheimer's mentioned above. Thus, the treatment site is the brain, for example, and the administration method is preferably direct administration to the brain, for example, by injection, nasal administration, or the like.
There is no particular limitation on the dosage form of the pharmaceutical composition of the present invention, and the dosage form may be set as appropriate according to the administration method. The dosage form of the pharmaceutical composition of the present invention at the time of administration is, for example, liquid, cream, gel, powder, or the like. Furthermore, the dosage form of the pharmaceutical composition of the present invention before administration, specifically the dosage form during the distribution process may be, for example, the same as or different from the dosage form at the time of administration. In the case of the latter, the dosage form may be, for example, a form that allows a pharmacist, a nurse, a physician, or the like at the time of administration to prepare the pharmaceutical composition into a dosage form for use at the time of administration. The dosage form before administration may be, for example, a powder, a solid such as granules, a concentrated liquid, or the like.
As described above, the additive components to the pharmaceutical composition of the present invention may be set as appropriate according to the administration method, the dosage form, and the like, and examples thereof include a solvent, a diluent, an excipient, and a carrier. The solvent may be, for example, an aqueous solvent such as water, saline solution, isotonic solution, or buffer solution, an oil solvent such as soybean oil, or an emulsion solvent, which is a mixed solution of an aqueous solvent and an oil solvent. The pharmaceutical composition of the present invention may contain, for example, alcohols, polyalcohols, surfactants, and the like, as the additive components. Furthermore, the pharmaceutical composition of the present invention may contain, for example, a DDS agent for effectively delivering the active component to the treatment site. The pharmaceutical composition of the present invention may be, for example, in a form containing a carrier in which the active component is encapsulated. The carrier may be, for example, nanoparticles such as polymers. The form in which the active component is encapsulated in this manner can maintain the stability of the active component, and also allow the carrier to function, for example, as a DDS. In this case, for example, the pharmaceutical composition of the present invention is preferably used for administration, for example, by intravenous injection or the like.
Examples of the administration target (test subject) of the pharmaceutical composition of the present invention include a human and a non-human animal. There is no particular limitation on the administration conditions of the pharmaceutical composition of the present invention, and the administration conditions may be determined as appropriate according to the species, the age, the weight, the sex, the presence or absence of amyloid-β aggregation diseases, the degree of progression, and the like. If the administration target is an adult male weighing 70 kg, the administration conditions of the pharmaceutical composition of the present invention are, for example, such that the total amount of Peptide (Peptide 1 and/or Peptide 2) per administration is 0.0005 to 100 mg, the administration frequency is once per day, and the interval is every 1 to 10 days.
In this specification, as described above, the meaning of treatment includes, for example, the meaning of prevention, inhibition of progression, and amelioration (alleviation). The pharmaceutical composition of the present invention may be used, for example, for any one or more of these purposes.
<Aggregation Inhibiting Method>
As described above, the method for inhibiting amyloid-β aggregation of the present invention is characterized by including adding the amyloid-β aggregation inhibitor of the present invention to a test subject. The inhibiting method of the present invention is characterized by using the aggregation inhibitor of the present invention, and there is no limitation on the other steps, conditions, or the like.
As for the addition of the aggregation inhibitor of the present invention to the test subject, reference can be made to the foregoing descriptions regarding the aggregation inhibitor and the pharmaceutical composition of the present invention. It is preferable that the method for inhibiting aggregation of the present invention further includes, for example, performing incubation, after adding the aggregation inhibitor of the present invention to the test subject. If the test subject is a non-living being, the incubation temperature is, for example, from room temperature to 37° C., the incubation time is from 4 to 72 hours, and the pH is from 6.5 to 8. Also, if the test subject is a cell or tissue, the incubation temperature is, for example, from room temperature to 37° C., the incubation time is from 1 to 7 days, and the pH is from 6.5 to 8.
<Method for Treating Amyloid-β Aggregation Disease>
As described above, the method for treating an amyloid-β aggregation disease of the present invention is characterized by administering the amyloid-β aggregation inhibitor of the present invention to a test subject. The treating method of the present invention is characterized by using the pharmaceutical composition of the present invention, and there is no limitation on the other steps, conditions, or the like.
As for the addition of the aggregation inhibitor of the present invention to the test subject, reference can be made to the foregoing descriptions regarding the aggregation inhibitor and the pharmaceutical composition of the present invention.
<Use of Peptide>
The peptide of the present invention is a peptide having an amino acid sequence of Sequence ID No. 1 or 2, for use in inhibiting aggregation of amyloid-β. Furthermore, the peptide of the present invention is a peptide having an amino acid sequence of Sequence ID No. 1 or 2, for use in treating amyloid-β aggregation diseases caused by aggregation of amyloid-β.
The ability of Peptide 1 (GSGNR) and Peptide 2 (GSGFK) to inhibit aggregation of amyloid-β (Aβ) was checked.
(1) Aβ Fragment Peptide
An Aβ-derived fragment peptide was used for aggregation of Aβ. Aβ25-35, which has high aggregability, was selected as the fragment peptide. The Aβ25th 35th is a peptide constituted by 11 amino acid residues consisting of the 25-th to 35-th amino acid residues in the full length sequence of human-derived Aβ (Sequence ID No. 4: GSNKGAIIGLM, hereinafter also referred to as Ab25-35).
(2) Aggregation Inhibition Assay
The fluorescent dye thioflavin T (ThT) binds to Ab aggregates and emits strong fluorescence upon binding, and thus the increase or inhibition of aggregation can be determined by measuring the fluorescence intensity. Thus, the inhibition of aggregation of Aβ25-35 by Peptide 1 (GSGNR) and Peptide 2 (GSGFK) was checked using ThT.
Specifically, a reaction solution with the following composition was prepared and dispensed into wells such that the amount of the reaction solution per well was 300 μL, and incubated at 37° C., and the change in fluorescence intensity over time was measured using a measurement device (product name Cytation5, manufactured by BioTek) (n=3). The measurement wavelengths were set such that the excitation wavelength (ex) was 444 nm and the emission wavelength (em) was 480 nm. As a control, a reaction solution to which water was added instead of Peptide 1 and Peptide 2 was also measured in the same way.
(3) Concentration Assay
The concentrations of Peptide 1 (GSGNR) and Peptide 2 (GSGFK) with respect to Aβ25-35 were checked. Unless otherwise indicated, measurements were performed according to (2) above.
First, as for Peptide 2 (GSGFK), 200 μL of mixed solution with the composition shown in Table 2 below was added per well, 10 μL of 2 mmol/L ThT was further added to the well immediately before measurement, and this reaction solution was incubated at 37° C. The change in fluorescence intensity 4 hours after the start of measurement was measured using the above-mentioned measurement device (n=3). Furthermore, reaction solutions with the following composition but with different final concentrations of Peptide 2 were also measured in the same way. As a negative control, a reaction solution with the following composition to which neither Peptides 1 nor 2 was added was also measured in the same way.
Next, as for Peptide 1 (GSGNR), 200 μL of mixed solution with the composition shown in Table 2 above was added per well, 10 μL of 2 mmol/L ThT was further added to the well immediately before measurement, and this reaction solution was incubated at 37° C. The change in fluorescence intensity 8 hours after the start of measurement was measured using the above-mentioned measurement device (n=6). Furthermore, reaction solutions with the composition above but with different final concentrations of Peptide 1 were also measured in the same way. As a negative control, a reaction solution with the composition above to which neither Peptides 1 nor 2 was added was also measured in the same way.
The effect of Peptide 2 (GSGFK) in vivo was checked. Note that physiological saline was used as a solvent for adjusting peptide or Aβ25-35 for administration.
In this example, 2 μL of 0.5 μg/μL Aβ25-35 and 2 μL of 0.25 μg/μL Peptide 2 were directly administered using a micro syringe to the CA1 area of the hippocampus of the brain of the administration group of C57BL/6 mice (7 to 13 months old mice) (n=3). In a similar manner, 2 μL of 0.5 μg/μL Aβ25-35 was directly administered using a micro syringe to the CA1 area of the hippocampus of the brain of the negative control group of C57BL/6 mice (n=3). Neither 2 μL of Aβ25-35 nor Peptide 2 was administered to the positive control group of C57BL/6 mice (n=3). Each group was then given a Y-maze (short-term memory assessment) test on days 11, 27, and 39 after administration, and an object recognition test over 3 days starting from day 49.
The Y-maze test was conducted in a general manner under the following conditions. That is to say, mice were placed such that their noses faced the wall at an end of one particular arm among the three arms. The mice were allowed to move freely for 10 minutes, and their behavior was captured on a monitor of an analyzer (product name: Time YM1, manufactured by O′hara & Co., Ltd.) to calculate the alternation behavior rate (alternation behavior (%)).
The object recognition test (ORT) from day 49 was conducted as follows. First, for the purpose of checking sociability, mice were placed in a square cage (with a width of 45 cm, a length of 45 cm, and a height of 15 cm) and allowed to become accustomed to the space over the course of one day. For each group, the speed of movement of the mice in the cage was measured, as well as the percentage (%) of time they stayed in the center region of the cage (the inner region being at least 7.5 cm away from a side wall of the cage).
After one day, two identical objects were placed in the cage and the mice were allowed to explore freely for one hour (sample phase). After one hour of exploration, the right side object of the two objects in the cage was replaced by a different object and the mice were again placed in the cage and allowed to explore freely (test phase). Also, the approach time of the mice to each object was measured in each of the sample and test phases. The percentage of the exploration time for the object on the right side with respect to the total exploration time was then determined (100× right side exploration time/total exploration time (%)).
In parallel with the administration to the mice in each group, the fluorescence intensity was measured for the solutions administered to each group. Specifically, a mixed solution obtained by mixing 0.5 μg/μL Aβ25-35 and 0.25 μg/μL Peptide 2 in equal amounts, which was the same as that administered to the administration group, and a 0.5 μg/μL Aβ25-35 solution, which was the same as that administered to the negative control group, were each added in an amount of 100 μL per well, and 10 μL of 1 mmol/L ThT was further added to the well immediately before measurement. These reaction solutions were each reacted at 37° C., and the fluorescence intensity was measured as in Example 1 above.
The ability of Peptide 1 (GSGNR) and Peptide 2 (GSGFK) to dissociate amyloid-β (Aβ) aggregates was checked.
A reaction solution with the following composition was prepared and incubated at 37° C. for 24 hours to form aggregates of Aβ25-35. After incubation, the fluorescence intensities of the reaction solution with the following composition to which Aβ25-35 was added and a reaction solution to which water was added instead of Aβ25-35 were measured as in Example 1. As a result, the fluorescence intensity of the reaction solution to which Aβ25-35 was added significantly increased compared with that of the reaction solution to which Aβ25-35 was not added, and thus it was found that aggregates of Aβ25-35 were formed.
Then, 40 μL of 10 mmol/L Peptide 1 or Peptide 2 was added to 4000 μL of the reaction solution in which the aggregates were formed (0 hr), the mixture was incubated at 37° C., and the change in fluorescence intensity over time was measured (n=3). As a control, a reaction solution to which water was added instead of Peptide 1 and Peptide 2 was also measured at 0 hr in the same way. Then, the percentage (%) of decrease in fluorescence intensity after 4 hr was obtained from the fluorescence intensity at 0 hr.
In the description above, the present invention was described by way of embodiments and examples, but the invention is not limited to the foregoing embodiments and examples. Various changes that can be understood by those skilled in the art can be made to the configuration and details of the invention within the scope of the invention.
This application claims the benefit of priority from Japanese Patent Application No. 2020-167404, filed on Oct. 2, 2020, the entire contents of which are incorporated herein by reference.
The amyloid-β aggregation inhibitor of the present invention can inhibit the aggregation of amyloid-β through intermolecular association, thus enabling treatment, e.g., prevention, inhibition of progression, and amelioration, of amyloid aggregation diseases such as Alzheimer's.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2020-167404 | Oct 2020 | JP | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/JP2021/036466 | 10/1/2021 | WO |
