Claims
- 1. A method for determining the rotational mobility of an analyte in a composition, the method comprising:
providing a composition that includes the analyte and a reference compound, the analyte and the reference compound being luminescent, the luminescence lifetimes of the analyte and reference compound being resolvable by lifetime-resolved methods; illuminating the composition, so that light is emitted by the analyte and reference compound; detecting the light emitted by the analyte and reference compound; calculating the rotational mobility of the light emitted by the analyte and the rotational mobility of the light emitted by the reference compound, based on the light that they emit and their luminescence lifetimes; and constructing a function that expresses the rotational mobility of the analyte relative to the rotational mobility of the reference compound.
- 2. The method of claim 1 further comprising calculating an amount of target substance in the composition based on the rotational mobility of the analyte.
- 3. A composition of matter comprising first and second luminophores, where the emission spectra of the first and second luminophores overlap significantly, and where light emitted by the first luminophore is resolvable from light emitted by the second luminophore using lifetime-resolved methods.
- 4. The composition of claim 3, where the lifetime-resolved methods include frequency-domain methods.
- 5. The composition of claim 4, where the light emitted by the second luminophore is indicative of light absorbing or scattering effects.
- 6. The composition of claim 3, where the first luminophore is an analyte, and the second luminophore is a reference compound.
- 7. The composition of claim 3 further comprising reagents, where the first luminophore reacts to indicate the amount of a target substance, and the second luminophore is indicative of light absorbing or scattering effects independent of how much target substance is present.
Priority Claims (5)
Number |
Date |
Country |
Kind |
PCT/US98/23095 |
Oct 1998 |
US |
|
PCT/US99/01656 |
Jan 1999 |
US |
|
PCT/US99/08410 |
Apr 1999 |
US |
|
PCT/US99/08410 |
Apr 1999 |
US |
|
PCT/US00/00895 |
Jan 2000 |
US |
|
CROSS-REFERENCES TO PRIORITY APPLICATIONS
[0001] The patents and patent applications listed below are incorporated herein by reference in their entirety for all purposes.
[0002] This application is a continuation-in-part of the following U.S. patent applications: Ser. No. 09/626,208, filed Jul. 26, 2000; Ser. No. 09/766,131, filed Jan. 19, 2001; Ser. No. 09/765,874, filed Jan. 19, 2001; Ser. No. 09/767,316, filed Jan. 22, 2001; Ser. No. 09/767,579, filed Jan. 22, 2001; Ser. No. 09/770,720, filed Jan. 25, 2001; and Ser. No. 09/722,247, filed Nov. 24, 2000.
[0003] U.S. patent application No. 09/626,208 is a continuation of PCT Patent Application Ser. No. PCT/US99/01656, filed Jan. 25, 1999. The '01656 application is a continuation-in-part of the following patent applications: U.S. patent application Ser. No. 09/062,472, filed Apr. 17, 1998; U.S. patent application Ser. No. 09/160,533, filed Sep. 24, 1998; and PCT Application Serail No. PCT/US98/23095, filed Oct. 30, 1998. These applications, in turn, claim priority from additional applications, as identified therein. This '01656 application also claims priority directly from the following U.S. provisional patent applications: Serial No. 60/072,499, filed Jan. 26, 1998; Ser. No. 60/072,780, filed Jan. 27, 1998; Ser. No. 60/075,806, filed Feb. 24, 1998; and Ser. No. 60/084,167, filed May 4, 1998.
[0004] U.S. patent application Ser. No. 09/766,131 is a continuation of PCT Patent Application Serial No. PCT/US99/16286, filed Jul. 26, 1999, which claims priority from U.S. Provisional Patent Application Ser. No. 60/094,306, filed Jul. 27, 1998.
[0005] U.S. patent application Ser. No. 09/765,874 is a continuation of PCT Patent Application Ser. No. PCT/US99/16287, filed Jul. 26, 1999, which claims priority from U.S. Provisional patent application Serial No. 60/094,276, filed Jul. 27, 1998.
[0006] U.S. patent application Ser. No. 09/767,316 is a continuation of PCT Patent Application Serial No. PCT/US00/00895, filed Jan. 14, 2000, which claims priority from the following U.S. provisional patent applications: Serial No. 60/116,113, filed Jan. 15, 1999; Ser. No. 60/135,284, filed May 21, 1999; and Ser. No. 60/167,463, filed Nov. 24, 1999.
[0007] U.S. patent application Ser. No. 09/767,579 is a continuation of PCT Patent Application Serial No. PCT/US00/04543, filed Feb. 22, 2000, which claims priority from U.S. Provisional Patent Application Serial No. 60/121,229, filed Feb. 23, 1999.
[0008] U.S. patent application Ser. No. 09/770,720 is a continuation of PCT Patent Application Serial No. PCT/US00/06841, filed Mar. 15, 2000. The '06841 application is a continuation-in-part of the following patent applications: PCT Patent Application Serial No. PCT/US99/08410, filed Apr. 16, 1999; U.S. patent application Ser. No. 09/349,733, filed Jul. 8, 1999; PCT Patent Application Serial No. PCT/US00/00895, filed Jan. 14, 2000; and U.S. patent application Ser. No. 09/494,401, filed Jan. 28, 2000. These applications, in turn, claim priority from additional applications, as identified therein. The '06841 application also claims priority directly from the following U.S. provisional patent applications: Serial No. 60/124,686, filed Mar. 16, 1999; Serial No. 60/125,346, filed Mar. 19, 1999; Serial No. 60/135,284, filed May 21, 1999; Serial No. 60/184,719, filed Feb. 24, 2000; and Serial No. 60/184,924, filed Feb. 25, 2000.
[0009] U.S. patent application Ser. No. 09/722,247 is a continuation-in-part of U.S. patent application Ser. No. 09/626,208, filed Jul. 26, 2000, which claims priority from additional applications, as indicated above. U.S. patent application Ser. No. 09/722,247 also claims priority directly from the following U.S. provisional patent applications: Serial No. 60/167,463, filed Nov. 24, 1999; and Serial No. 60/182,419, filed Feb. 14, 2000.
Provisional Applications (6)
|
Number |
Date |
Country |
|
60094306 |
Jul 1998 |
US |
|
60094276 |
Jul 1998 |
US |
|
60116113 |
Jan 1999 |
US |
|
60135284 |
May 1999 |
US |
|
60167463 |
Nov 1999 |
US |
|
60121229 |
Feb 1999 |
US |
Continuation in Parts (9)
|
Number |
Date |
Country |
Parent |
09626208 |
Jul 2000 |
US |
Child |
10012255 |
Nov 2001 |
US |
Parent |
09766131 |
Jan 2001 |
US |
Child |
10012255 |
Nov 2001 |
US |
Parent |
09765874 |
Jan 2001 |
US |
Child |
10012255 |
Nov 2001 |
US |
Parent |
09767316 |
Jan 2001 |
US |
Child |
10012255 |
Nov 2001 |
US |
Parent |
09767579 |
Jan 2001 |
US |
Child |
10012255 |
Nov 2001 |
US |
Parent |
09770720 |
Jan 2001 |
US |
Child |
10012255 |
Nov 2001 |
US |
Parent |
09722247 |
Nov 2000 |
US |
Child |
10012255 |
Nov 2001 |
US |
Parent |
09062472 |
Apr 1998 |
US |
Child |
PCT/US99/01656 |
Jan 1999 |
US |
Parent |
09160533 |
Sep 1998 |
US |
Child |
PCT/US99/01656 |
Jan 1999 |
US |