Claims
- 1. A isolated nucleic acid molecule comprising a nucleotide sequence encoding a protein comprising an amino acid sequence at least 90% identical to the amino acid sequence of SEQ ID NO:2, wherein said protein is capable of binding phosphoinositides.
- 2. The isolated nucleic acid molecule of claim 1, comprising the nucleotide sequence of SEQ ID NO:1.
- 3. The isolated nucleic acid molecule of claim 1, comprising the coding region of the nucleotide sequence of SEQ ID NO: 1.
- 4. The isolated nucleic acid molecule of claim 1 encoding the amino acid sequence of SEQ ID NO:2.
- 5. The isolated nucleic acid of claim 1, wherein the protein binds phosphotidylinositol-3,4,5 and is involved in cell adhesion.
- 6. The isolated nucleic acid of claim 1, wherein the protein binds phosphotidylinositol-3,4,5 and is involved in membrane trafficking.
- 7. The isolated nucleic acid of claim 1, wherein the protein binds phosphotidylinositol-3,4,5 and and modulates nucleotide exchange activity of a GTP-binding protein.
- 8. The isolated nucleic acid of claim 7, wherein the GTP-binding protein is an ARF protein.
- 9. An isolated nucleic acid molecule comprising a nucleotide sequence encoding a PH domain which is at least 95% identical to a Pleastrin Homology (PH) amino acid sequence of SEQ ID NO:2, said PH domain being capable of binding phosphonidylinositol-3,4,5.
- 10. The isolated nucleic acid molecule of claim 9 wherein said PH domain is the PH domain of SEQ ID NO:2.
- 11. The isolated nucleic acid molecule of claim 9, wherein said PH domain comprises amino acid residues 258 to 384 of SEQ ID NO:2.
- 12. An isolated nucleic acid molecule comprising a nucleotide sequence encoding a SEC7 domain which is at least 95% identical to a SEC7 domain of amino acid sequence of SEQ ID NO:2, said SEC7 domain being capable of mediating cell adhesion.
- 13. An isolated nucleic acid molecule comprising a nucleotide sequence encoding a SEC7 domain which is at least 95%o identical to a SEC7 domain of the amino acid sequence of SEQ ID NO:2, said SEC7 domain being capable of modulating the nucleotide exchange activity of a GTP-binding protein.
- 14. The isolated nucleic acid molecule of claim 9, wherein said SEC7 domain is the SEC7 domain of SEQ ID NO:2.
- 15. The isolated nucleic acid molecule of claim 9, wherein said SEC7 domain comprises amino acid residues 74 to 252 of SEQ ID NO:2.
- 16. An isolated nucleic acid molecule encoding GRP which is capable of hybridizing to The nucleic acid of SEQ ID NO:1 in 6.0× SSC at 50° C. followed by washing in 0.2× SSC at 65° C.
- 17. The isolated nucleic acid molecule of claim 16 which comprises a naturally-occurring nucleotide sequence.
- 18. The isolated nucleic acid molecule of claim 16 which encodes human GRP.
- 19. The isolated nucleic acid molecule of claim 16 which encodes mouse GRP.
- 20. A vector comprising a nucleic acid molecule of claim 1, 9, 12, 13 or 16.
- 21. The vector of claim 20, which is a recombinant expression vector.
- 22. A host cell containing the vector of claim 20.
- 23. A host cell containing the vector of claim 21.
- 24. A method for producing GRP or portions thereof comprising culturing the host cell of claim 22 in a suitable medium until GRP is produced.
- 25. An isolated nucleic acid molecule encoding a protein comprising a PH domain wherein said nucleic acid molecule comprises a nucleic acid sequence including residues 772-1150 of SEQ ID NO:1 and said PH domain is capable of binding phosphotidylinositol-3,4,5.
- 26. An isolated nucleic acid molecule encoding a protein comprising a SEC7 domain wherein said nucleic acid molecule comprises a nucleic acid sequence including residues 217-754 of SEQ ID NO:1 and said SEC7 domain is capable of mediating cell adhesion.
Parent Case Info
This application is a national stage entry of PCT/US97/18152 application filed Oct. 7, 1997, which is a Continuation of U.S. Ser. No. 08/729,834, filed Oct. 7, 1996.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
102e Date |
371c Date |
PCT/US97/18152 |
|
WO |
00 |
7/26/1999 |
7/26/1999 |
Publishing Document |
Publishing Date |
Country |
Kind |
WO98/15629 |
4/16/1998 |
WO |
A |
Continuations (1)
|
Number |
Date |
Country |
Parent |
08/729834 |
Oct 1996 |
US |
Child |
09/284033 |
|
US |