Claims
- 1. A cDNA molecule which encodes a human CIF130 protein comprising an amino acid sequence which is at least 85% identical to the amino acid sequence shown in SEQ ID NO:2, wherein said protein binds to and negatively regulates CIF150/hTAFII150.
- 2. The cDNA molecule of claim 1 which encodes at least 17 contiguous amino acids of SEQ ID NO:2.
- 3. The cDNA molecule of claim 2 which encodes SEQ ID NO:2.
- 4. The cDNA molecule of claim 3 which comprises SEQ ID NO:1.
- 5. The cDNA molecule of claim 1 comprising at least 12 contiguous nucleotides of SEQ ID NO:1.
- 6. A cDNA molecule which is at least 85% identical to the nucleotide sequence shown in SEQ ID NO:1, wherein said cDNA molecule encodes a CIF130 protein that binds to and negatively regulates CIF150/hTAFII150.
- 7. An isolated and purified subgenomic polynucleotide comprising a nucleotide sequence which hybridizes to SEQ ID NO:1 after washing with 0.2×SSC at 65° C., wherein the nucleotide sequence encodes a CIF130 protein having the amino acid sequence of SEQ ID NO:2.
- 8. A construct comprising:a promoter; and a polynucleotide segment encoding at least 17 contiguous amino acids of the human CIF130 protein as shown in SEQ ID NO:2, wherein the polynucleotide segment is located downstream from the promoter, and wherein transcription of the polynucleotide segment initiates at the promoter.
- 9. A host cell comprising the construct of claim 8.
- 10. A homologously recombinant cell having incorporated therein a new transcription initiation unit, wherein the new transcription initiation unit comprises:(a) an exogenous regulatory sequence; (b) an exogenous exon; and (c) a splice donor site, wherein the transcription initiation unit is located upstream of the coding sequence of the CIF130 gene as shown in SEQ ID NO:1, wherein the exogenous regulatory sequence directs transcription of the coding sequence of the CIF130 gene.
- 11. The homologously recombinant cell of claim 10 wherein the exogenous regulatory sequence directs enhanced transcription of the coding sequence of the CIF130 gene.
- 12. The cDNA molecule of claim 1, wherein said protein comprises an amino acid sequence which is at least 90% identical to the amino acid sequence shown in SEQ ID NO:2.
- 13. The cDNA molecule of claim 1, wherein said protein comprises an amino acid sequence which is at least 95% identical to the amino acid sequence shown in SEQ ID NO:2.
- 14. The cDNA molecule of claim 1, wherein said protein comprises an amino acid sequence which is at least 98% identical to the amino acid sequence shown in SEQ ID NO:2.
- 15. The cDNA molecule of claim 6, wherein said cDNA molecule is at least 90% identical to the nucleotide sequence shown in SEQ ID NO:1.
- 16. The cDNA molecule of claim 6, wherein said cDNA molecule is at least 95% identical to the nucleotide sequence shown in SEQ ID NO:1.
- 17. The cDNA molecule of claim 6, wherein said cDNA molecule is at least 98% identical to the nucleotide sequence shown in SEQ ID NO:1.
Parent Case Info
This application claims the benefit of co-pending provisional applications Ser. No. 60/089,198 filed Jun. 12, 1998, and Ser. No. 60/111,636 filed Dec. 9, 1998, which are incorporated herein by reference.
Non-Patent Literature Citations (3)
Entry |
Kaufmann and Smale, “Direct recognition of initiator elements by a component of the transcription factor IID complex,” Genes & Development 8: 821-829, 1994. |
Kaufmann et al., “CIF150, a Human Cofactor for Transcription Factor IID-Dependent Initiator Function,” Molecular and Cellular Biology 18(1): 233-239, 1998. |
Kaufmann et al., “CIF, an essential cofactor for TFIID-dependent initiator function,” Genes & Development 10: 873-886, 1996. |
Provisional Applications (2)
|
Number |
Date |
Country |
|
60/089198 |
Jun 1998 |
US |
|
60/111636 |
Dec 1998 |
US |