Claims
- 1. A method for the recombinant expression of bryodin 1 comprising transfecting a host cell with an expression vector comprising an oligonucleotide sequence encoding the contiguous amino acid sequence of Sequence ID#2, growing the transfected host cells, inducing the transfected host cells to express the bryodin 1 and isolating the expressed recombinant bryodin 1.
- 2. The method of claim 1 wherein the host cell is a bacteria, a plant cell, a yeast, or a mammalian cell.
- 3. A method for producing a recombinant bryodin 1-ligand fusion protein comprising transfecting a host cell with an expression vector comprising an oligonucleotide sequence encoding the contiguous amino acid sequence of Sequence ID#2 operatively linked with an oligonucleotide sequence which encodes a ligand, growing the transfected host cells, inducing the transfected host cells to express the recombinant bryodin 1-ligand fusion protein, and isolating the expressed recombinant bryodin 1-ligand fusion protein.
- 4. The method of claim 3, wherein the host cell is a bacteria, a plant, a yeast, or a mammalian cell.
- 5. The method of claim 4, wherein the ligand is a polypeptide, or a peptide ligand.
- 6. The method of claim 4, wherein the ligand is an immunoreactive ligand, transferrin, an epidermal growth factor, bombesin, gastrin, gastrin-releasing peptide, platelet-derived growth factor, IL-2, IL-6, or a tumor growth factor.
- 7. The method of claim 6, wherein the immunoreactive ligand is an antigen-binding immunoglobulin, or an antigen-binding fragment thereof, a chimeric antibody, a bifunctional antibody, a hybrid antibody or a single chain antibody.
- 8. The method of claim 7, wherein the antigen-binding fragment of the immunoreactive ligand is a Fab', F(ab').sub.2, Fv or Fab fragment of an immunoglobulin.
- 9. The method of claim 5, wherein the polypeptide is a large molecular weight protein.
- 10. The method of claim 5, wherein the polypeptide is a small molecular weight protein.
Parent Case Info
This application is a divisional of application Ser. No. 08/245,754, filed May 17, 1994 issued as U.S. Pat. No. 5,541,110.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5541110 |
Siegall |
Jul 1996 |
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Divisions (1)
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Number |
Date |
Country |
Parent |
245754 |
May 1994 |
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