Claims
- 1. An isolated and purified recombinant nucleic acid comprising:
- (i) a first nucleotide sequence shown in FIG. 1 (SEQ ID NO: 1); and
- (ii) a nucleotide sequence which is 100% complementary to the nucleotide sequence shown in FIG. 1 (SEQ ID NO: 1), wherein said nucleotide sequence is a full length sequence.
- 2. An isolated and purified recombinant nucleic acid comprising:
- a first nucleotide sequence comprising an open reading frame that encodes a chicken anemia virus protein, wherein said first nucleotide sequence is selected from the group consisting of nucleotides 380-1028, and nucleotides 486-849 of the sequence shown in FIG. 1 (SEQ ID NO: 1).
- 3. An isolated and purified recombinant nucleic acid according to any one of claim 1, or claim 2, further comprising a second nucleotide sequence that encodes a regulatory region operatively linked to said first nucleotide sequence.
- 4. The isolated and purified recombinant nucleic acid according to claim 3, further comprising:
- a third nucleotide sequence, wherein said third nucleotide sequence is not derived from a chicken anemia virus genome.
- 5. The isolated and purified recombinant nucleic acid according to claim 4, wherein said third nucleotide sequence is derived from a prokaryotic or an eukaryotic expression vector.
- 6. The isolated and purified recombinant nucleic acid according to claim 4, wherein said third nucleotide sequence encodes a protein other than a chicken anemia virus protein.
- 7. The isolated and purified recombinant nucleic acid according to claim 4, wherein said third nucleotide sequence encodes a regulatory region.
- 8. The isolated and purified recombinant nucleic acid according to claim 1, wherein said nucleic acid is DNA.
- 9. The isolated and purified recombinant nucleic acid according to claim 8, wherein said DNA is labeled with one or more markers suitable for labeling DNA.
- 10. The isolated and purified recombinant nucleic acid according to claim 9, wherein said markers are selected from the group consisting of radioisotopes, enzymes, haptens, fluorescent substances, dyes, and pigments.
- 11. The isolated and purified recombinant nucleic acid according to claim 1, wherein said nucleic acid is RNA.
- 12. The isolated and purified recombinant nucleic acid according to claim 11, wherein said RNA is labeled with one or more markers suitable for labeling RNA.
- 13. The isolated and purified recombinant nucleic acid according to claim 12, wherein said markers are selected from the group consisting of enzymes, haptens, fluorescent substances, dyes, and pigments.
- 14. A prokaryotic or eukaryotic cell containing an isolated and purified recombinant nucleic acid according to claim 3, wherein said recombinant nucleic acid further comprises a third nucleotide sequence not derived from a chicken anemia virus genome.
- 15. The prokaryotic or eukaryotic cell according to claim 14, wherein said cell expresses at least one protein encoded by said recombinant nucleic acid.
- 16. An isolated and purified recombinant nucleic acid comprising:
- a nucleotide sequence of 18 to 28 nucleotides in length which specifically hybridizes under stringent hybridization conditions to a nucleotide sequence which comprises an open reading frame that encodes a chicken anemia virus protein.
- 17. The isolated and purified recombinant nucleic acid according to claim 16, wherein said nucleotide sequence is selected from the group consisting of nucleotides 853-2200, nucleotides 380-1028, and nucleotides 486-849 of the sequence shown in FIG. 1 (SEQ ID NO: 1).
- 18. The isolated and purified recombinant nucleic acid according to claim 16, wherein said nucleotide sequence is shown in FIG. 4 (SEQ ID NO: 2).
- 19. An isolated and purified double-stranded nucleic acid, wherein a plus strand of said nucleic acid comprises a first nucleotide sequence comprising an open reading frame that encodes a chicken anemia virus protein wherein said first nucleotide sequence is selected from the group consisting of nucleotides 380-1028 and nucleotides 486-849 of the sequence shown in FIG. 1 (SEQ ID NO: 1).
- 20. An expression vector comprising:
- a nucleic acid according to claim 19 wherein said first nucleotide sequence is operatively linked to a second nucleotide sequence encoding a regulatory region functional in an avian cell, wherein said second nucleotide sequence is not derived from a chicken anemia virus genome.
- 21. A method of introducing a nucleic acid derived from a chicken anemia virus genome into an avian cell, said method comprising:
- contacting said avian cell with an expression vector comprising as operatively linked components, a regulatory region functional in an avian cell and a nucleic acid having the nucleotide sequence shown in FIG. 1 (SEQ ID NO: 1), whereby said nucleic acid is introduced into said avian cell.
- 22. An expression vector comprising:
- as operatively linked components, a regulatory region functional in an avian cell and one or more open reading frames having a nucleotide sequence selected from the group consisting of nucleotides 380-1028, and nucleotides 486-849 of the sequence shown in FIG. 1 (SEQ ID NO: 1).
- 23. An expression vector comprising:
- as operatively linked components a regulatory region functional in a host cell and a nucleotide sequence encoding a CAV protein wherein said first nucleotide sequence is selected from the group consisting of nucleotides 380-1028 and nucleotides 486-849 of the sequence shown in FIG. 1 (SEQ ID NO: 1).
- 24. The expression vector according to claim 23, wherein said host cell is a eukaryotic cell.
- 25. The expression vector according to claim 24, wherein said eukaryotic cell is an insect cell.
- 26. The expression vector according to claim 25, wherein said regulatory region is a baculovirus promoter.
- 27. The expression vector according to claim 23, wherein said CAV protein is encoded by a nucleotide sequence selected from the group consisting of nucleotides 380-1029 and nucleotides 486-849 of the sequence shown in FIG. 2a (SEQ ID NO:1).
- 28. A method of introducing a first nucleotide sequence into an avian cell, said method comprising:
- introducing into said avian cell an expression vector according to claim 20, whereby said first nucleotide sequence is introduced into said avian cell.
- 29. The method according to claim 28, wherein said expression vector further comprises a marker.
- 30. A ribonucleic acid which is 100% complementary to the nucleotide sequence shown in FIG. 1 (SEQ ID NO: 1).
- 31. The isolated and purified recombinant nucleic acid according to claim 2, wherein said nucleic acid is DNA.
Priority Claims (1)
Number |
Date |
Country |
Kind |
9002008 |
Sep 1990 |
NLX |
|
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation-in-part of U.S. Ser. No. 08/030,335 filed Mar. 8, 1993 (now U.S. Pat. No. 5,496,073) which disclosure is incorporated herein by reference. U.S. Ser. No. 08/030,335 is a U.S. national stage application of PCT/NL91/00165, filed Sep. 11, 1991. Other related applications include U.S. Ser. No. 08/474,450, filed Jun. 7, 1995, U.S. Ser. No. 08/454,121, filed Jun. 7, 1995, U.S. Ser. No. 08/482,161, filed Jun. 7, 1995, U.S. Ser. No. 08,485,001, filed Jun. 7, 1995, and U.S. Ser. No. 08/489,666, filed Jun. 7, 1995.
Foreign Referenced Citations (1)
Number |
Date |
Country |
0483911 |
May 1992 |
EPX |
Non-Patent Literature Citations (18)
Entry |
Chiou et al., (1992) Nucleic Acids Res. 20:1717-1723. |
Innis & Gelfand, (1990) in: PCR Protocols: A Guide to Methods and Applications, Eds., Innis et al., Academic Press Inc., San Diego, CA, pp. 3-20. |
Noteborn et al., (1991) J. Virol. 65:3131-3139. |
Noteborn et al., (1992) Avian Pathology 21:107-118. |
Ritchie et al., (1989) Virology 171:83-88. |
Than & Stanislawek, (1992) 3 Arch. Virol. 127:245-255. |
Todd et al., (1990) J. General Virology 71:819-823. |
Jeurissen et al., (1992) J. Virol. 66:7383-7388. |
Noteborn et al., (1992a) Gene 118:267-271. |
Todd et al., (1991) Arch. Virol. 117:129-135. |
Buchholtz (1994) Charakterisierung des Huhneranamievirus(CAV) mit Hilfe von monoklonalen Antikorpern, Journal-Nr. 1738. |
Chiou et al., (1994) J. Virol. 68:6553-6566. |
Gelderblom et al. (1989) Arch. Virol. 109: 115-120. |
Noteborn et al. (1992) in: Seminar organized for the European Commission from Dec. 15 to 16, 1992: New and Evolving Virus Diseases of Poultry, Eds., McNulty and McFerran, pp. 195-213. |
Noteborn et al. (1993) in : Vaccines 93, Cold Spring Harbor Laboratory Press, pp. 299-304. |
Ramakrishnan et al. (1993) Nature 362:219-223. |
Shi-Mei Zhuang et al. (1995) Cancer Research 55:486-489. |
Hanold et al. Journal General Virology (1988) 69:1323-1329. |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
030335 |
|
|