Claims
- 1. A system for identifying particulate matter, such as a cell, comprising
- means for producing coherent light,
- flow-through means for passing a supply of particulate matter therethrough,
- means for focusing light from said coherent light means through said flow-through means to produce a diffraction pattern wherein the light is scattered by particles of said particulate matter at angles characteristic of said matter,
- means positioned to receive said light scattered at characteristic angles for sensing light scattered at different angles and for producing a set of angle characteristic signal voltages in response thereto,
- pulse generating means responsive to scattered light for detecting the presence of a scattering particle in said beam and for producing a delayed pulse,
- peak sample and hold means responsive to said delayed pulse for sampling the peak output voltages of said light sensing means in response to the one scattering particle, wherein each peak output voltage is proportional to the peak intensity of light scattered at a different angle,
- means establishing a set of reference voltages representative of the characteristic set of peak output voltages produced by particulate matter desired to be identified, and
- means for comparing the set of characteristic peak output voltages produced by said sample and hold means with the set of reference voltages, and for producing an output when there is an identity between the set of characteristic peak voltages and the set of reference voltages.
- 2. A system as recited in claim 1 wherein said light sensing means comprises a plurality of spaced concentric partial rings of photovoltaic material, and
- said means for comparing includes means for comparing the characteristic voltage output produced by each concentric partial ring with a reference voltage.
- 3. A system as recited in claim 1 wherein said means establishing a set of reference voltages comprises means for establishing a set of reference voltage ranges, and
- said means for comparing compares the set of characteristic peak voltages with said set of reference voltage ranges.
- 4. A system as recited in claim 1 wherein there is included means for displaying the set of characteristic peak voltages.
- 5. A system as recited in claim 1 wherein said light means comprises a plurality of spaced concentric arcs of photovoltaic material, only predetermined ones of which are located to receive the light scattered at angles characteristic of said matter, and
- said means for comparing includes means for selecting only the peak voltage output from each of said predetermined rings for comparison with said set of reference voltages.
- 6. A method as recited in claim 1 wherein said means establishing a set of reference voltages comprises
- means for storing a plurality of reference sets of voltages, and
- means for sequentially extracting each reference set of voltages from said means for storing for enabling a sequential comparison with a set of characteristic peak voltages.
- 7. A system for identifying a cell as recited in claim 1 wherein there is included oscilloscope means to which said plurality of characteristic peak voltages are applied for displaying said characteristic voltages as light images.
- 8. A method of identifying particulate matter, such as a cell, comprising
- illuminating a particle of said matter with a beam of coherent light to produce a diffraction pattern wherein the light is scattered by said particle at angles characteristic of said matter,
- generating a set of peak voltages representative of, and in response to, the light scattered at angles characteristic of said matter,
- establishing a reference set of voltages representative of the light scattered by a particle of known matter, and
- comparing the set of peak voltages representative of the light scattered at angles characteristic of said matter with said reference set of voltages and producing an output indicative of an identity.
- 9. A method as recited in claim 8 wherein said step of establishing a reference set of voltages includes establishing a reference set of voltage tolerance ranges, and
- said set of comparing compares the set of peak voltages with a reference set of voltage tolerance ranges.
- 10. A method of particle identification as recited in claim 8 wherein said step of generating a set of peak voltages representative of and in response to the light scattered at angles characteristic of a particle of said matter includes
- placing a plurality of separate photovoltaic materials at locations where they will intercept the light scattered at angles, and
- deriving a separate peak voltage from each of said plurality of separate photovoltaic materials.
- 11. A method of cell identification as recited in claim 8 including displaying said plurality of peak voltages representative of the light scattered at characteristic angles.
- 12. A method for identifying particulate matter as recited in claim 8 wherein said step of establishing a reference set of voltages includes
- storing a plurality of reference sets of voltages,
- sequentially establishing from said stored reference sets of voltages each different set of voltages for comparing with said generated set of voltages.
ORIGIN OF THE INVENTION
The invention described herein was made in the performance of work under an NIH Grant RR00443.
US Referenced Citations (9)