Compositions and methods for the treatment and diagnosis of breast cancer

Abstract

Compositions and methods for the detection and therapy of breast cancer are disclosed. The compounds provided include nucleotide sequences that are preferentially expressed in breast tumor tissue, as well as polypeptides encoded by such nucleotide sequences. Vaccines and pharmaceutical compositions comprising such compounds are also provided and may be used, for example, for the prevention and treatment of breast cancer. The polypeptides may also be used for the production of antibodies, which are useful for diagnosing and monitoring the progression of breast cancer in a patient.

Description

TECHNICAL FIELD

The present invention relates generally to the detection and therapy of breast cancer. The invention is more specifically related to nucleotide sequences that are preferentially expressed in breast tumor tissue and to polypeptides encoded by such nucleotide sequences. The nucleotide sequences and polypeptides may be used in vaccines and pharmaceutical compositions for the prevention and treatment of breast cancer. The polypeptides may also be used for the production of compounds, such as antibodies, useful for diagnosing and monitoring the progression of breast cancer in a patient.

BACKGROUND OF THE INVENTION

Breast cancer is a significant health problem for women in the United States and throughout the world. Although advances have been made in detection and treatment of the disease, breast cancer remains the second leading cause of cancer-related deaths in women, affecting more than 180,000 women in the United States each year. For women in North America, the life-time odds of getting breast cancer are now one in eight.

No vaccine or other universally successful method for the prevention or treatment of breast cancer is currently available. Management of the disease currently relies on a combination of early diagnosis (through routine breast screening procedures) and aggressive treatment, which may include one or more of a variety of treatments such as surgery, radiotherapy, chemotherapy and hormone therapy. The course of treatment for a particular breast cancer is often selected based on a variety of prognostic parameters, including an analysis of specific tumor markers. See, e.g., Porter-Jordan and Lippman, Breast Cancer 8:73-100 (1994). However, the use of established markers often leads to a result that is difficult to interpret, and the high mortality observed in breast cancer patients indicates that improvements are needed in the treatment, diagnosis and prevention of the disease.

Accordingly, there is a need in the art for improved methods for therapy and diagnosis of breast cancer. The present invention fulfills these needs and further provides other related advantages.

SUMMARY OF THE INVENTION

Briefly stated, the subject invention provides compositions and methods for the diagnosis and therapy of breast cancer. In one aspect, isolated polynucleotides are provided, comprising (a) a nucleotide sequence preferentially expressed in breast cancer tissue, relative to normal tissue; (b) a variant of such a sequence, as defined below; or (c) a nucleotide sequence encoding an epitope of a polypeptide encoded by at least one of the above sequences. In one embodiment, the isolated polynucleotide comprises a human endogenous retroviral sequence recited in SEQ ID NO:1. In other embodiments, the isolated polynucleotide comprises a sequence recited in any one of SEQ ID NO: 3-26, 28-77, 142, 143, 146-152, 154-166, 168-176, 178-192, 194-198, 200-204, 206, 207, 209-214, 216, 218, 219, 221-240, 243-245, 247, 250, 251, 253, 255, 257-266, 268, 269, 271-273, 275, 276, 278, 280, 281, 284, 288, 291-298, 301-303, 307, 313 and 314.

In related embodiments, the isolated polynucleotide encodes an epitope of a polypeptide, wherein the polypeptide is encoded by a nucleotide sequence that: (a) hybridizes to a sequence recited in any one of SEQ ID NO: 1, 3-26, 28-77, 142, 143, 146-152, 154-166, 168-176, 178-192, 194-198, 200-204, 206, 207, 209-214, 216, 218, 219, 221-240, 243-245, 247, 250, 251, 253, 255, 257-266, 268, 269, 271-273, 275, 276, 278, 280, 281, 284, 288, 291-298, 301-303, 307, 313 and 314 under stringent conditions; and (b) is at least 80% identical to a sequence recited in any one of SEQ ID NO: 1, 3-26, 28-77, 142, 143, 146-152, 154-166, 168-176, 178-192, 194-198, 200-204, 206, 207, 209-214, 216, 218, 219, 221-240, 243-245, 247, 250, 251, 253, 255, 257-266, 268, 269, 271-273, 275, 276, 278, 280, 281, 284, 288, 291-298, 301-303, 307, 313 and 314.

In another embodiment, the present invention provides an isolated polynucleotide encoding an epitope of a polypeptide, the polypeptide being encoded by: (a) a nucleotide sequence transcribed from the sequence of SEQ ID NO: 141; or (b) a variant of said nucleotide sequence that contains one or more nucleotide substitutions, deletions, insertions and/or modifications at no more than 20% of the nucleotide positions, such that the antigenic and/or immunogenic properties of the polypeptide encoded by the nucleotide sequence are retained. Isolated DNA and RNA molecules comprising a nucleotide sequence complementary to a polynucleotide as described above are also provided.

In related aspects, the present invention provides recombinant expression vectors comprising a polynucleotide as described above and host cells transformed or transfected with such expression vectors.

In further aspects, polypeptides comprising an amino acid sequence encoded by a polynucleotide as described above, and monoclonal antibodies that bind to such polypeptides are provided. In certain embodiments, the inventive polypeptides comprise an amino acid sequence selected from the group consisting of SEQ ID NO: 299, 300, 304-306, 308 and 315, and variants thereof as defined below.

In yet another aspect, methods are provided for determining the presence of breast cancer in a patient. In one embodiment, the method comprises detecting, within a biological sample, a polypeptide as described above. In another embodiment, the method comprises detecting, within a biological sample, an RNA molecule encoding a polypeptide as described above. In yet another embodiment, the method comprises (a) intradermally injecting a patient with a polypeptide as described above; and (b) detecting an immune response on the patient's skin and therefrom detecting the presence of breast cancer in the patient. In further embodiments, the present invention provides methods for determining the presence of breast cancer in a patient as described above wherein the polypeptide is encoded by a nucleotide sequence selected from the group consisting of SEQ ID NO: 78-86, 144, 145, 153, 167, 177, 193, 199, 205, 208, 215, 217, 220, 241, 242, 246, 248, 249, 252, 256, 267, 270, 274, 277, 279, 282, 283, 285-287, 289, 290 and sequences that hybridize thereto under stringent conditions.

In a related aspect, diagnostic kits useful in the determination of breast cancer are provided. The diagnostic kits generally comprise either one or more monoclonal antibodies as described above, or one or more monoclonal antibodies that bind to a polypeptide encoded by a nucleotide sequence selected from the group consisting of sequences provided in SEQ ID NO: 78-86, 144, 145, 153, 167, 177, 193, 199, 205, 208, 215, 217, 220, 241, 242 and 246, 248, 249, 252, 256, 267, 270, 274, 277, 279, 282, 283, 285-287, 289, 290 and a detection reagent.

Diagnostic kits are also provided that comprise a first polymerase chain reaction primer and a second polymerase chain reaction primer, at least one of the primers being specific for a polynucleotide described herein. In one embodiment, at least one of the primers comprises at least about 10 contiguous nucleotides of a polynucleotide as described above, or a polynucleotide encoding a polypeptide encoded by a sequence selected from the group consisting of SEQ ID NO: 78-86, 144, 145, 153, 167, 177, 193, 199, 205, 208, 215, 217, 220, 241, 242, 246, 248, 249, 252, 256, 267, 270, 274, 277, 279, 282, 283, 285-287, 289 and 290.

Within another related aspect, the diagnostic kit comprises at least one oligonucleotide probe, the probe being specific for a polynucleotide described herein. In one embodiment, the probe comprises at least about 15 contiguous nucleotides of a polynucleotide as described above, or a polynucleotide selected from the group consisting of SEQ ID NO: 78-86, 144, 145, 153, 167, 177, 193, 199, 205, 208, 215, 217, 220, 241, 242 246, 248, 249, 252, 256, 267, 270, 274, 277, 279, 282, 283, 285-287, 289 and 290.

In another related aspect, the present invention provides methods for monitoring the progression of breast cancer in a patient. In one embodiment, the method comprises: (a) detecting an amount, in a biological sample, of a polypeptide as described above at a first point in time; (b) repeating step (a) at a subsequent point in time; and (c) comparing the amounts of polypeptide detected in steps (a) and (b), and therefrom monitoring the progression of breast cancer in the patient. In another embodiment, the method comprises (a) detecting an amount, within a biological sample, of an RNA molecule encoding a polypeptide as described above at a first point in time; (b) repeating step (a) at a subsequent point in time; and (c) comparing the amounts of RNA molecules detected in steps (a) and (b), and therefrom monitoring the progression of breast cancer in the patient. In yet other embodiments, the present invention provides methods for monitoring the progression of breast cancer in a patient as described above wherein the polypeptide is encoded by a nucleotide sequence selected from the group consisting of SEQ ID NO: 78-86, 144, 145, 153, 167, 177, 193, 199, 205, 208, 215, 217, 220, 241, 242, 246, 248, 249, 252, 256, 267, 270, 274, 277, 279, 282, 283, 285-287, 289, 290 and sequences that hybridize thereto under stringent conditions.

In still other aspects, pharmaceutical compositions, which comprise a polypeptide as described above in combination with a physiologically acceptable carrier, and vaccines, which comprise a polypeptide as described above in combination with an immune response enhancer or adjuvant, are provided. In yet other aspects, the present invention provides pharmaceutical compositions and vaccines comprising a polypeptide encoded by a nucleotide sequence selected from the group consisting of SEQ ID NO: 78-86, 144, 145, 153, 167, 177, 193, 199, 205, 208, 215, 217, 220, 241, 242 and 246, 248, 249, 252, 256, 267, 270, 274, 277, 279, 282, 283, 285-287, 289, 290 and sequences that hybridize thereto under stringent conditions.

In related, aspects, the present invention provides methods for inhibiting the development of breast cancer in a patient, comprising administering to a patient a pharmaceutical composition or vaccine as described above.

These and other aspects of the present invention will become apparent upon reference to the following detailed description and attached drawings. All references disclosed herein are hereby incorporated by reference in their entirety as if each was incorporated individually.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the differential display PCR products, separated by gel electrophoresis, obtained from cDNA prepared from normal breast tissue (lanes 1 and 2) and from cDNA prepared from breast tumor tissue from the same patient (lanes 3 and 4). The arrow indicates the band corresponding to B18Ag1.

FIG. 2 is a northern blot comparing the level of B18Ag1 mRNA in breast tumor tissue (lane 1) with the level in normal breast tissue.

FIG. 3 shows the level of B18Ag1 mRNA in breast tumor tissue compared to that in various normal and non-breast tumor tissues as determined by RNase protection assays.

FIG. 4 is a genomic clone map showing the location of additional retroviral sequences obtained from ends of XbaI restriction digests (provided in SEQ ID NO:3-SEQ ID NO:10) relative to B18Ag1.

FIGS. 5A and 5B show the sequencing strategy, genomic organization and predicted open reading frame for the retroviral element containing B18Ag1 (SEQ ID NO: 1).

FIG. 6 shows the nucleotide sequence of the representative breast tumor-specific cDNA B18Ag1 (SEQ ID NO: 11).

FIG. 7 shows the nucleotide sequence of the representative breast tumor-specific cDNA B17Ag1 (SEQ ID NO: 12).

FIG. 8 shows the nucleotide sequence of the representative breast tumor-specific cDNA B17Ag2 (SEQ ID NO: 13).

FIG. 9 shows the nucleotide sequence of the representative breast tumor-specific cDNA B13Ag2a (SEQ ID NO: 14).

FIG. 10 shows the nucleotide sequence of the representative breast tumor-specific cDNA B13Ag1b (SEQ ID NO: 15).

FIG. 11 shows the nucleotide sequence of the representative breast tumor-specific cDNA B13Ag1a (SEQ ID NO: 16).

FIG. 12 shows the nucleotide sequence of the representative breast tumor-specific cDNA B11Ag1 (SEQ ID NO: 17).

FIG. 13 shows the nucleotide sequence of the representative breast tumor-specific cDNA B3CA3c (SEQ ID NO: 18).

FIG. 14 shows the nucleotide sequence of the representative breast tumor-specific cDNA B9CG1 (SEQ ID NO: 19).

FIG. 15 shows the nucleotide sequence of the representative breast tumor-specific cDNA B9CG3 (SEQ ID NO: 20).

FIG. 16 shows the nucleotide sequence of the representative breast tumor-specific cDNA B2CA2 (SEQ ID NO: 21).

FIG. 17 shows the nucleotide sequence of the representative breast tumor-specific cDNA B3CA1 (SEQ ID NO: 22).

FIG. 18 shows the nucleotide sequence of the representative breast tumor-specific cDNA B3CA2 (SEQ ID NO: 23).

FIG. 19 shows the nucleotide sequence of the representative breast tumor-specific cDNA B3CA3 (SEQ ID NO: 24).

FIG. 20 shows the nucleotide sequence of the representative breast tumor-specific cDNA B4CA1.

FIG. 21A depicts RT-PCR analysis of breast tumor genes in breast tumor tissues (lanes 1-8) and normal breast tissues (lanes 9-13) and H 2 O (lane 14).

FIG. 21B depicts RT-PCR analysis of breast tumor genes in prostate tumors (lane 1, 2), colon tumors (lane 3), lung tumor (lane 4), normal prostate (lane 5), normal colon (lane 6), normal kidney (lane 7), normal liver (lane 8), normal lung (lane 9), normal ovary (lanes 10, 18), normal pancreases (lanes 11, 12), normal skeletal muscle (lane 13), normal skin (lane 14), normal stomach (lane 15), normal testes (lane 16), normal small intestine (lane 17), HBL-100 (lane 19), MCF-12A (lane 20), breast tumors (lanes 21-23), H 2 O (lane 24), and colon tumor (lane 25).

FIG. 22 shows the recognition of a B11Ag1 peptide (referred to as B11-8) by an anti-B11-8 CTL line.

FIG. 23 shows the recognition of a cell line transduced with the antigen B11Ag1 by the B11-8 specific clone A1.

FIG. 24 shows recognition of a lung adenocarcinoma line (LT-140-22) and a breast adenocarcinoma line (CAMA-1) by the B11-8 specific clone A1.

DETAILED DESCRIPTION OF THE INVENTION

As noted above, the present invention is generally directed to compositions and methods for the diagnosis, monitoring and therapy of breast cancer. The compositions described herein include polypeptides, polynucleotides and antibodies. Polypeptides of the present invention generally comprise at least a portion of a protein that is expressed at a greater level in human breast tumor tissue than in normal breast tissue (i.e., the level of RNA encoding the polypeptide is at least 2-fold higher in tumor tissue). Such polypeptides are referred to herein as breast tumor-specific polypeptides, and cDNA molecules encoding such polypeptides are referred to as breast tumor-specific cDNAs. Polynucleotides of the subject invention generally comprise a DNA or RNA sequence that encodes all or a portion of a polypeptide as described above, or that is complementary to such a sequence. Antibodies are generally immune system proteins, or fragments thereof, that are capable of binding to a portion of a polypeptide as described above. Antibodies can be produced by cell culture techniques, including the generation of monoclonal antibodies as described herein, or via transfection of antibody genes into suitable bacterial or mammalian cell hosts, in order to allow for the production of recombinant antibodies.

Polypeptides within the scope of this invention include, but are not limited to, polypeptides (and epitopes thereof) encoded by a human endogenous retroviral sequence, such as the sequence designated B18Ag1 (FIG. 5 and SEQ ID NO:1). Also within the scope of the present invention are polypeptides encoded by other sequences within the retroviral genome containing B18Ag1 (SEQ ID NO: 141). Such sequences include, but are not limited to, the sequences recited in SEQ ID NO:3-SEQ ID NO: 10. B18Ag1 has homology to the gag p30 gene of the endogenous human retroviral element S71, as described in Werner et al., Virology 174:225-238 (1990) and also shows homology to about thirty other retroviral gag genes. As discussed in more detail below, the present invention also includes a number of additional breast tumor-specific polypeptides, such as those encoded by the nucleotide sequences recited in SEQ ID NO: 11-26, 28-77, 142, 143, 146-152, 154-166, 168-176, 178-192, 194-198, 200-204, 206, 207, 209-214, 216, 218, 219, 221-240, 243-245, 247, 250, 251, 253, 255, 257-266, 268, 269, 271-273, 275, 276, 278, 280, 281, 284, 288, 291-298, 301-303, 307, 313 and 314.

As used herein, the term “polypeptide” encompasses amino acid chains of any length, including full length proteins containing the sequences recited herein. A polypeptide comprising an epitope of a protein containing a sequence as described herein may consist entirely of the epitope, or may contain additional sequences. The additional sequences may be derived from the native protein or may be heterologous, and such sequences may (but need not) possess immunogenic or antigenic properties.

An “epitope,” as used herein is a portion of a polypeptide that is recognized (i.e., specifically bound) by a B-cell and/or T-cell surface antigen receptor. Epitopes may generally be identified using well known techniques, such as those summarized in Paul, Fundamental Immunology, 3rd ed., 243-247 (Raven Press, 1993) and references cited therein. Such techniques include screening polypeptides derived from the native polypeptide for the ability to react with antigen-specific antisera and/or T-cell lines or clones. An epitope of a polypeptide is a portion that reacts with such antisera and/or T-cells at a level that is similar to the reactivity of the full length polypeptide (e.g., in an ELISA and/or T-cell reactivity assay). Such screens may generally be performed using methods well known to those of ordinary skill in the art, such as those described in Harlow and Lane, Antibodies: A Laboratory Manual , Cold Spring Harbor Laboratory, 1988. B-cell and T-cell epitopes may also be predicted via computer analysis. Polypeptides comprising an epitope of a polypeptide that is preferentially expressed in a tumor tissue (with or without additional amino acid sequence) are within the scope of the present invention.

The term “polynucleotide(s),” as used herein, means a single or double-stranded polymer of deoxyribonucleotide or ribonucleotide bases and includes DNA and corresponding RNA molecules, including HnRNA and mRNA molecules, both sense and anti-sense strands, and comprehends cDNA, genomic DNA and recombinant DNA, as well as wholly or partially synthesized polynucleotides. An HnRNA molecule contains introns and corresponds to a DNA molecule in a generally one-to-one manner. An mRNA molecule corresponds to an HnRNA and DNA molecule from which the introns have been excised. A polynucleotide may consist of an entire gene, or any portion thereof. Operable anti-sense polynucleotides may comprise a fragment of the corresponding polynucleotide, and the definition of “polynucleotide” therefore includes all such operable anti-sense fragments.

The compositions and methods of the present invention also encompass variants of the above polypeptides and polynucleotides.

A polypeptide “variant,” as used herein, is a polypeptide that differs from the recited polypeptide only in conservative substitutions and/or modifications, such that the antigenic properties of the polypeptide are retained. In a preferred embodiment, variant polypeptides differ from an identified sequence by substitution, deletion or addition of five amino acids or fewer. Such variants may generally be identified by modifying one of the above polypeptide sequences, and evaluating the antigenic properties of the modified polypeptide using, for example, the representative procedures described herein. Polypeptide variants preferably exhibit at least about 70%, more preferably at least about 90% and most preferably at least about 95% identity (determined as described below) to the identified polypeptides.

As used herein, a “conservative substitution” is one in which an amino acid is substituted for another amino acid that has similar properties, such that one skilled in the art of peptide chemistry would expect the secondary structure and hydropathic nature of the polypeptide to be substantially unchanged. In general, the following groups of amino acids represent conservative changes: (1) ala, pro, gly, glu, asp, gln, asn, ser, thr; (2) cys, ser, tyr, thr; (3) val, ile, leu, met, ala, phe; (4) lys, arg, his; and (5) phe, tyr, trp, his.

Variants may also, or alternatively, contain other modifications, including the deletion or addition of amino acids that have minimal influence on the antigenic properties, secondary structure and hydropathic nature of the polypeptide. For example, a polypeptide may be conjugated to a signal (or leader) sequence at the N-terminal end of the protein which co-translationally or post-translationally directs transfer of the protein. The polypeptide may also be conjugated to a linker or other sequence for ease of synthesis, purification or identification of the polypeptide (e.g., poly-His), or to enhance binding of the polypeptide to a solid support. For example, a polypeptide may be conjugated to an immunoglobulin Fc region.

A nucleotide “variant” is a sequence that differs from the recited nucleotide sequence in having one or more nucleotide deletions, substitutions or additions. Such modifications may be readily introduced using standard mutagenesis techniques, such as oligonucleotide-directed site-specific mutagenesis as taught, for example, by Adelman et al. ( DNA, 2:183, 1983). Nucleotide variants may be naturally occurring allelic variants, or non-naturally occurring variants. Variant nucleotide sequences preferably exhibit at least about 70%, more preferably at least about 80% and most preferably at least about 90% identity (determined as described below) to the recited sequence.

The breast tumor antigens provided by the present invention include variants that are encoded by DNA sequences which are substantially homologous to one or more of the DNA sequences specifically recited herein. “Substantial homology,” as used herein, refers to DNA sequences that are capable of hybridizing under moderately stringent conditions. Suitable moderately stringent conditions include prewashing in a solution of 5× SSC, 0.5% SDS, 1.0 mM EDTA (pH 8.0); hybridizing at 50° C. -65° C., 5× SSC, overnight or, in the event of cross-species homology, at 45° C. with 0.5× SSC; followed by washing twice at 65° C. for 20 minutes with each of 2×, 0.5× and 0.2× SSC containing 0.1% SDS. Such hybridizing DNA sequences are also within the scope of this invention, as are nucleotide sequences that, due to code degeneracy, encode an immunogenic polypeptide that is encoded by a hybridizing DNA sequence.

Two nucleotide or polypeptide sequences are said to be “identical” if the sequence of nucleotides or amino acid residues in the two sequences is the same when aligned for maximum correspondence as described below. Comparisons between two sequences are typically performed by comparing the sequences over a comparison window to identify and compare local regions of sequence similarity. A “comparison window” as used herein, refers to a segment of at least about 20 contiguous positions, usually 30 to about 75, 40 to about 50, in which a sequence may be compared to a reference sequence of the same number of contiguous positions after the two sequences are optimally aligned.

Optimal alignment of sequences for comparison may be conducted using the Megalign program in the Lasergene suite of bioinformatics software (DNASTAR, Inc., Madison, Wis.), using default parameters. This program embodies several alignment schemes described in the following references: Dayhoff, M. O. (1978) A model of evolutionary change in proteins—Matrices for detecting distant relationships. In Dayhoff, M. O. (ed.) Atlas of Protein Sequence and Structure, National Biomedical Resarch Foundaiton, Washington D.C. Vol. 5, Suppl. 3, pp. 345-358; Hein J. (1990) Unified Approach to Alignment and Phylogenes pp. 626-645 Methods in Enzymology vol. 183, Academic Press, Inc., San Diego, Calif.; Higgins, D. G. and Sharp, P. M. (1989) Fast and sensitive multiple sequence alignments on a microcomputer CABIOS 5:151-153; Myers, E. W. and Muller W. (1988) Optimal alignments in linear space CABIOS 4:11-17; Robinson, E. D. (1971) Comb. Theor 11:105; Santou, N. Nes, M. (1987) The neighbor joining method. A new method for reconstructing phylogenetic trees Mol. Biol. Evol. 4:406-425; Sneath, P. H. A. and Sokal, R. R. (1973) Numerical Taxonomy—the Principles and Practice of Numerical. Taxonomy , Freeman Press, San Francisco, Calif.; Wilbur, W. J. and Lipman, D. J. (1983) Rapid similarity searches of nucleic acid and protein data banks Proc. Natl. Acad., Sci. USA 80:726-730.

Preferably, the “percentage of sequence identity” is determined by comparing two optimally aligned sequences over, a window of comparison of at least 20 positions, wherein the portion of the polynucleotide sequence in the comparison window may comprise additions or deletions (i.e. gaps) of 20 percent or less, usually 5 to 15 percent, or 10 to 12 percent, as compared to the reference sequences (which does not comprise additions or deletions) for optimal alignment of the two sequences. The percentage is calculated by determining the number of positions at which the identical nucleic acid bases or amino acid residue occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the reference sequence (i.e. the window size) and multiplying the results by 100 to yield the percentage of sequence identity. In general, polynucleotides encoding all or a portion of the polypeptides described herein may be prepared using any of several techniques. For example, cDNA molecules encoding such polypeptides may be cloned on the basis of the breast tumor-specific expression of the corresponding mRNAs, using differential display PCR. This technique compares the amplified products from RNA template prepared from normal and breast tumor tissue. cDNA may be prepared by reverse transcription of RNA using a (dT) 12 AG primer. Following amplification of the cDNA using a random primer, a band corresponding to an amplified product specific to the tumor RNA may be cut out from a silver stained gel and subcloned into a suitable vector (e.g., the T-vector, Novagen, Madison, Wis.). Polynucleotides encoding all or a portion of the breast tumor-specific polypeptides disclosed herein may be amplified from cDNA prepared as described above using the random primers shown in SEQ ID NO.:87-125.

Alternatively, a polynucleotide encoding a polypeptide as described herein (or a portion thereof) may be amplified from human genomic DNA, or from breast tumor cDNA, via polymerase chain reaction. For this approach, B18Ag1 sequence-specific primers may be designed based on the sequence provided in SEQ ID NO:1, and may be purchased or synthesized. One suitable primer pair for amplification from breast tumor cDNA is (5′ATG GCT ATT TTC GGG GGC TGA CA) (SEQ ID NO:126) and (5° CCG GTA TCT CCT CGT GGG TAT T) (SEQ ID NO:127). An amplified portion of B18Ag1 may then be used to isolate the full length gene from a human genomic DNA library or from a breast tumor cDNA library, using well known techniques, such as those described in Sambrook et al., Molecular Cloning: A Laboratory Manual , Cold Spring Harbor Laboratories, Cold Spring Harbor, N.Y. (1989). Other sequences within the retroviral genome of which B18Ag1 is a part may be similarly prepared by screening human genomic libraries using B18Ag1-specific sequences as probes. Nucleotides translated into protein from the retroviral genome shown in SEQ ID NO: 141 may then be determined by cloning the corresponding cDNAs, predicting the open reading frames and cloning the appropriate cDNAs into a vector containing a viral promoter, such as T7. The resulting constructs can be employed in a translation reaction, using techniques known to those of skill in the art, to identify nucleotide sequences which result in expressed protein. Similarly, primers specific for the remaining breast tumor-specific polypeptides described herein may be designed based on the nucleotide sequences provided in SEQ ID NO: 11-86, 142-298, 301-303, 307, 313 and 314.

Recombinant polypeptides encoded by the DNA sequences described above may be readily prepared from the DNA sequences. For example, supernatants from suitable host/vector systems which secrete recombinant protein or polypeptide into culture media may be first concentrated using a commercially available filter. Following concentration, the concentrate may be applied to a suitable purification matrix such as an affinity matrix or an ion exchange resin. Finally, one or more reverse phase HPLC steps can be employed to further purify a recombinant polypeptide.

In general, any of a variety of expression vectors known to those of ordinary skill in the art may be employed to express recombinant polypeptides of this invention. Expression may be achieved in any appropriate host cell that has been transformed or transfected with an expression vector containing a polynucleotide that encodes a recombinant polypeptide. Suitable host cells include prokaryotes, yeast and higher eukaryotic cells. Preferably, the host cells employed are E. coli, yeast or a mammalian cell line such as COS or CHO.

Such techniques may also be used to prepare polypeptides comprising epitopes or variants of the native polypeptides. For example, variants of a native polypeptide may generally be prepared using standard mutagenesis techniques, such as oligonucleotide-directed site-specific mutagenesis, and sections of the DNA sequence may be removed to permit preparation of truncated polypeptides. Portions and other variants having fewer than about 100 amino acids, and generally fewer than about 50 amino acids, may also be generated by synthetic means, using techniques well known to those of ordinary skill in the art. For example, such polypeptides may be synthesized using any of the commercially available solid-phase techniques, such as the Merrifield solid-phase synthesis method, where amino acids are sequentially added to a growing amino acid chain. See Merrifield, J. Am. Chem. Soc. 85:2149-2146 (1963). Equipment for automated synthesis of polypeptides is commercially available from suppliers such as Perkin Elmer/Applied BioSystems Division, Foster City, Calif., and may be operated according to the manufacturer's instructions.

In specific embodiments, polypeptides of the present invention encompass amino acid sequences encoded by a polynucleotide having a sequence recited in any one of SEQ ID NO:1, 3-26, 28-77, 142, 143, 146-152, 154-166, 168-176, 178-192, 194-198, 200-204, 206, 207, 209-214, 216, 218, 219, 221-240, 243-245, 247, 250, 251, 253, 255, 257-266, 268, 269, 271-273, 275, 276, 278, 280, 281, 284, 288, 291-298, 301-303, 307, 313 and 314, and variants of such polypeptides. Polypeptides within the scope of the present invention also include polypeptides (and epitopes thereof) encoded by DNA sequences that hybridize to a sequence recited in any one of SEQ ID NO:1, 3-26, 28-77, 142, 143, 146-152, 154-166, 168-176, 178-192, 194-198, 200-204, 206, 207, 209-214, 216, 218, 219, 221-240, 243-245, 247, 250, 251, 253, 255, 257-266, 268, 269, 271-273, 275, 276, 278, 280, 281, 284, 288, 291-298, 301-303, 307, 313 and 314 under stringent conditions, wherein the DNA sequences are at least 80% identical in overall sequence to a recited sequence and wherein RNA corresponding to the nucleotide sequence is expressed at a greater level in human breast tumor tissue than in normal breast tissue. As used herein, “stringent conditions” refers to prewashing in a solution of 6× SSC, 0.2% SDS; hybridizing at 65° C, 6× SSC, 0.2% SDS overnight; followed by two washes of 30 minutes each in 1× SSC, 0.1% SDS at 65° C. and two washes of 30 minutes each in 0.2× SSC, 0.1% SDS at 65° C. Polynucleotides according to the present invention include molecules that encode any of the above polypeptides.

In another aspect of the present invention, antibodies are provided. Such antibodies may be prepared by any of a variety of techniques known to those of ordinary skill in the art. See, e.g., Harlow and Lane, Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, 1988. In one such technique, an immunogen comprising the polypeptide is initially injected into any of a wide variety of mammals (e.g., mice, rats, rabbits, sheep or goats). In this step, the polypeptides of this invention may serve as the immunogen without modification. Alternatively, particularly for relatively short polypeptides, a superior immune response may be elicited if the polypeptide is joined to a carrier protein, such as bovine serum albumin or keyhole limpet hemocyanin. The immunogen is injected into the animal host, preferably according to a predetermined schedule incorporating one or more booster immunizations, and the animals are bled periodically. Polyclonal antibodies specific for the polypeptide may then be purified from such antisera by, for example, affinity chromatography using the polypeptide coupled to a suitable solid support.

Monoclonal antibodies specific for the antigenic polypeptide of interest may be prepared, for example, using the technique of Kohler and Milstein, Eur. J. Immunol. 6:511-519 (1976), and improvements thereto. Briefly, these methods involve the preparation of immortal cell lines capable of producing antibodies having the desired specificity (i.e., reactivity with the polypeptide of interest). Such cell lines may be produced, for example, from spleen cells obtained from an animal immunized as described above. The spleen cells are then immortalized by, for example, fusion with a myeloma cell fusion partner, preferably one that is syngeneic with the immunized animal. A variety of fusion techniques may be employed. For example, the spleen cells and myeloma cells may be combined with a nonionic detergent for a few minutes and then plated at low density on a selective medium that supports the growth of hybrid cells, but not myeloma cells. A preferred selection technique uses HAT (hypoxanthine, aminopterin, thymidine) selection. After a sufficient time, usually about 1 to 2 weeks, colonies of hybrids are observed. Single colonies are selected and their culture supernatants tested for binding activity against the polypeptide. Hybridomas having high reactivity and specificity are preferred.

Monoclonal antibodies may be isolated from the supernatants of growing hybridoma colonies. In addition, various techniques may be employed to enhance the yield, such as injection of the hybridoma cell line into the peritoneal cavity of a suitable vertebrate host, such as a mouse. Monoclonal antibodies may then be harvested from the ascites fluid or the blood. Contaminants may be removed from the antibodies by conventional techniques, such as chromatography, gel filtration, precipitation, and extraction. The polypeptides of this invention may be used in the purification process in, for example, an affinity chromatography step.

Antibodies may be used, for example, in methods for detecting breast cancer in a patient. Such methods involve using an antibody to detect the presence or absence of a breast tumor-specific polypeptide as described herein in a suitable biological sample. As used herein, suitable biological samples include tumor or normal tissue biopsy, mastectomy, blood, lymph node, serum or urine samples, or other tissue, homogenate, or extract thereof obtained from a patient.

There are a variety of assay formats known to those of ordinary skill in the art for using an antibody to detect polypeptide markers in a sample. See, e.g., Harlow and Lane, Antibodies: A Laboratory Manual , Cold Spring Harbor Laboratory, 1988. For example, the assay may be performed in a Western blot format, wherein a protein preparation from the biological sample is submitted to gel electrophoresis, transferred to a suitable membrane and allowed to react with the antibody. The presence of the antibody on the membrane may then be detected using a suitable detection reagent, as described below.

In another embodiment, the assay involves the use of antibody immobilized on a solid support to bind to the polypeptide and remove it from the remainder of the sample. The bound polypeptide may then be detected using a second antibody or reagent that contains a reporter group. Alternatively, a competitive assay may be utilized, in which a polypeptide is labeled with a reporter group and allowed to bind to the immobilized antibody after incubation of the antibody with the sample. The extent to which components of the sample inhibit the binding of the labeled polypeptide to the antibody is indicative of the reactivity of the sample with the immobilized antibody, and as a result, indicative of the concentration of polypeptide in the sample.

The solid support may be any material known to those of ordinary skill in the art to which the antibody may be attached. For example, the solid support may be a test well in a microtiter plate or a nitrocellulose filter or other suitable membrane. Alternatively, the support may be a bead or disc, such as glass, fiberglass, latex or a plastic material such as polystyrene or polyvinylchloride. The support may also be a magnetic particle or a fiber optic sensor, such as those disclosed, for example, in U.S. Pat. No. 5,359,681.

The antibody may be immobilized on the solid support using a variety of techniques known to those in the art, which are amply described in the patent and scientific literature. In the context of the present invention, the term “immobilization” refers to both noncovalent association, such as adsorption, and covalent attachment (which may be a direct linkage between the antigen and functional groups on the support or may be a linkage by way of a cross-linking agent). Immobilization by adsorption to a well in a microtiter plate or to a membrane is preferred. In such cases, adsorption may be achieved by contacting the antibody, in a suitable buffer, with the solid support for a suitable amount of time. The contact time varies with temperature, but is typically between about 1 hour and 1 day. In general, contacting a well of a plastic microtiter plate (such as polystyrene or polyvinylchloride) with an amount of antibody ranging from about 10 ng to about 1 μg, and preferably about 100-200 ng, is sufficient to immobilize an adequate amount of polypeptide.

Covalent attachment of antibody to a solid support may also generally be achieved by first reacting the support with a bifunctional reagent that will react with both the support and a functional group, such as a hydroxyl or amino group, on the antibody. For example, the antibody may be covalently attached to supports having an appropriate polymer coating using benzoquinone or by condensation of an aldehyde group on the support with an amine and an active hydrogen on the binding partner (see, e.g., Pierce Immunotechnology Catalog and Handbook (1991) at A12-A13).

In certain embodiments, the assay for detection of polypeptide in a sample is a two-antibody sandwich assay. This assay may be performed by first contacting an antibody that has been immobilized on a solid support, commonly the well of a microtiter plate, with the biological sample, such that the polypeptide within the sample are allowed to bind to the immobilized antibody. Unbound sample is then removed from the immobilized polypeptide-antibody complexes and a second antibody (containing a reporter group) capable of binding to a different site on the polypeptide is added. The amount of second antibody that remains bound to the solid support is then determined using a method appropriate for the specific reporter group.

More specifically, once the antibody is immobilized on the support as described above, the remaining protein binding sites on the support are typically blocked. Any suitable blocking agent known to those of ordinary skill in the art, such as bovine serum albumin or Tween 20™ (Sigma Chemical Co., St. Louis, Mo.). The immobilized antibody is then incubated with the sample, and polypeptide is allowed to bind to the antibody. The sample may be diluted with a suitable diluent, such as phosphate-buffered saline (PBS) prior to incubation. In general, an appropriate contact time (i.e., incubation time) is that period of time that is sufficient to detect the presence of polypeptide within a sample obtained from an individual with breast cancer. Preferably, the contact time is sufficient to achieve a level of binding that is at least 95% of that achieved at equilibrium between bound and unbound polypeptide. Those of ordinary skill in the art will recognize that the time necessary to achieve equilibrium may be readily determined by assaying the level of binding that occurs over a period of time. At room temperature, an incubation time of about 30 minutes is generally sufficient.

Unbound sample may then be removed by washing the solid support with an appropriate buffer, such as PBS containing 0.1% Tween 20™. The second antibody, which contains a reporter group, may then be added to the solid support. Preferred reporter groups include enzymes (such as horseradish peroxidase), substrates, cofactors, inhibitors, dyes, radionuclides, luminescent groups, fluorescent groups and biotin. The conjugation of antibody to reporter group may be achieved using standard methods known to those of ordinary skill in the art.

The second antibody is then incubated with the immobilized antibody-polypeptide complex for an amount of time sufficient to detect the bound polypeptide. An appropriate amount of time may generally be determined by assaying the level of binding that occurs over a period of time. Unbound second antibody is then removed and bound second antibody is detected using the reporter group. The method employed for detecting the reporter group depends upon the nature of the reporter group. For radioactive groups, scintillation counting or autoradiographic methods are generally appropriate. Spectroscopic methods may be used to detect dyes, luminescent groups and fluorescent groups. Biotin may be detected using avidin, coupled to a different reporter group (commonly a radioactive or fluorescent group or an enzyme). Enzyme reporter groups may generally be detected by the addition of substrate (generally for a specific period of time), followed by spectroscopic or other analysis of the reaction products.

To determine the presence or absence of breast cancer, the signal detected from the reporter group that remains bound to the solid support is generally compared to a signal that corresponds to a predetermined cut-off value established from non-tumor tissue. In one preferred embodiment, the cut-off value is the average mean signal obtained when the immobilized antibody is incubated with samples from patients without breast cancer. In general, a sample generating a signal that is three standard deviations above the predetermined cut-off value may be considered positive for breast cancer. In an alternate preferred embodiment, the cut-off value is determined using a Receiver Operator Curve, according to the method of Sackett et al., Clinical Epidemiology: A Basic Science for Clinical Medicine , p. 106-7 (Little Brown and Co., 1985). Briefly, in this embodiment, the cut-off value may be determined from a plot of pairs of true positive rates (i.e., sensitivity) and false positive rates (100%-specificity) that correspond to each possible cut-off value for the diagnostic test result. The cut-off value on the plot that is the closest to the upper left-hand corner (i.e., the value that encloses the largest area) is the most accurate cut-off value, and a sample generating a signal that is higher than the cut-off value determined by this method may be considered positive. Alternatively, the cut-off value may be shifted to the left along the plot, to minimize the false positive rate, or to the right, to minimize the false negative rate. In general, a sample generating a signal that is higher than the cut-off value determined by this method is considered positive for breast cancer.

In a related embodiment, the assay is performed in a flow-through or strip test format, wherein the antibody is immobilized on a membrane, such as nitrocellulose. In the flow-through test, the polypeptide within the sample bind to the immobilized antibody as the sample passes through the membrane. A second, labeled antibody then binds to the antibody-polypeptide complex as a solution containing the second antibody flows through the membrane. The detection of bound second antibody may then be performed as described above. In the strip test format, one end of the membrane to which antibody is bound is immersed in a solution containing the sample. The sample migrates along the membrane through a region containing second antibody and to the area of immobilized antibody. Concentration of second antibody at the area of immobilized antibody indicates the presence of breast cancer. Typically, the concentration of second antibody at that site generates a pattern, such as a line, that can be read visually. The absence of such a pattern indicates a negative result. In general, the amount of antibody immobilized on the membrane is selected to generate a visually discernible pattern when the biological sample contains a level of polypeptide that would be sufficient to generate a positive signal in the two-antibody sandwich assay, in the format discussed above. Preferably, the amount of antibody immobilized on the membrane ranges from about 25 ng to about 1 μg, and more preferably from about 50 ng to about 1 μg. Such tests can typically be performed with a very small amount of biological sample.

The presence or absence of breast cancer in a patient may also be determined by evaluating the level of mRNA encoding a breast tumor-specific polypeptide as described herein within the biological sample (e.g., a biopsy, mastectomy and/or blood sample from a patient) relative to a predetermined cut-off value. Such an evaluation may be achieved using any of a variety of methods known to those of ordinary skill in the art such as, for example, in situ hybridization and amplification by polymerase chain reaction.

For example, polymerase chain reaction may be used to amplify sequences from cDNA prepared from RNA that is isolated from one of the above biological samples. Sequence-specific primers for use in such amplification may be designed based on the sequences provided in any one of SEQ ID NO: 1, 11-86, 142-298 301-303, 307, 313 and 314, and may be purchased or synthesized. In the case of B18Ag1, as noted herein, one suitable primer pair is B18Ag1-2 (5′ATG GCT ATT TTC GGG GGC TGA CA) (SEQ ID NO:126) and B18Ag1-3 (5° CCG GTA TCT CCT CGT GGG TAT T) (SEQ ID NO: 127). The PCR reaction products may then be separated by gel electrophoresis and visualized according to methods well known to those of ordinary skill in the art. Amplification is typically performed on samples obtained from matched pairs of tissue (tumor and non-tumor tissue from the same individual) or from unmatched pairs of tissue (tumor and non-tumor tissue from different individuals). The amplification reaction is preferably performed on several dilutions of cDNA spanning two orders of magnitude. A two-fold or greater increase in expression in several dilutions of the tumor sample as compared to the same dilution of the non-tumor sample is considered positive.

As used herein, the term “primer/probe specific for a polynucleotide” means an oligonucleotide sequence that has at least about 80% identity, preferably at least about 90% and more preferably at least about 95%, identity to the polynucleotide in question, or an oligonucleotide sequence that is anti-sense to a sequence that has at least about 80% identity, preferably at least about 90% and more preferably at least about 95%, identity to the polynucleotide in question. Primers and/or probes which may be usefully employed in the inventive diagnostic methods preferably have at least about 10-40 nucleotides. In a preferred embodiment, the polymerase chain reaction primers comprise at least about 10 contiguous nucleotides of a polynucleotide that encodes one of the polypeptides disclosed herein or that is anti-sense to a sequence that encodes one of the polypeptides disclosed herein. Preferably, oligonucleotide probes for use in the inventive diagnostic methods comprise at least about 15 contiguous oligonucleotides of a polynucleotide that encodes one of the polypeptides disclosed herein or that is anti-sense to a sequence that encodes one of the polypeptides disclosed herein. Techniques for both PCR based assays and in situ hybridization assays are well known in the art.

Conventional RT-PCR protocols using agarose and ethidium bromide staining, while important in defining gene specificity, do not lend themselves to diagnostic kit development because of the time and effort required in making them quantitative (i.e., construction of saturation and/or titration curves), and their sample throughput. This problem is overcome by the development of procedures such as real time RT-PCR which allows for assays to be performed in single tubes, and in turn can be modified for use in 96 well plate formats. Instrumentation to perform such methodologies are available from Perkin Elmer/Applied Biosystems Division. Alternatively, other high throughput assays using labeled probes (e.g., digoxygenin) in combination with labeled (e.g., enzyme fluorescent, radioactive) antibodies to such probes can also be used in the development of 96 well plate assays.

In yet another method for determining the presence or absence of breast cancer in a patient, one or more of the breast tumor-specific polypeptides described may be used in a skin test. As used herein, a “skin test” is any assay performed directly on a patient in which a delayed-type hypersensitivity (DTH) reaction (such as swelling, reddening or dermatitis) is measured following intradermal injection of one or more polypeptides as described above. Such injection may be achieved using any suitable device sufficient to contact the polypeptide or polypeptides with dermal cells of the patient, such as a tuberculin syringe or 1 mL syringe. Preferably, the reaction is measured at least 48 hours after injection, more preferably 48-72 hours.

The DTH reaction is a cell-mediated immune response, which is greater in patients that have been exposed previously to a test antigen (i.e., an immunogenic portion of a polypeptide employed, or a variant thereof). The response may measured visually, using a ruler. In general, a response that is greater than about 0.5 cm in diameter, preferably greater than about 5.0 cm in diameter, is a positive response, indicative of breast cancer.

The breast tumor-specific polypeptides described herein are preferably formulated, for use in a skin test, as pharmaceutical compositions containing at least one polypeptide and a physiologically acceptable carrier, such as water, saline, alcohol, or a buffer. Such compositions typically contain one or more of the above polypeptides in an amount ranging from about 1 μg to 100 μg, preferably from about 10 μg to 50 μg in a volume of 0.1 mL. Preferably, the carrier employed in such pharmaceutical compositions is a saline solution with appropriate preservatives, such as phenol and/or Tween 80™.

In other aspects of the present invention, the progression and/or response to treatment of a breast cancer may be monitored by performing any of the above assays over a period of time, and evaluating the change in the level of the response (i.e., the amount of polypeptide or mRNA detected or, in the case of a skin test, the extent of the immune response detected). For example, the assays may be performed every month to every other month for a period of 1 to 2 years. In general, breast cancer is progressing in those patients in whom the level of the response increases over time. In contrast, breast cancer is not progressing when the signal detected either remains constant or decreases with time.

In further aspects of the present invention, the compounds described herein may be used for the immunotherapy of breast cancer. In these aspects, the compounds (which may be polypeptides, antibodies or polynucleotides) are preferably incorporated into pharmaceutical compositions or vaccines. Pharmaceutical compositions comprise one or more such compounds and a physiologically acceptable carrier. Vaccines may comprise one or more such compounds and an immune response enhancer, such as an adjuvant or a liposome (into which the compound is incorporated). Pharmaceutical compositions and vaccines may additionally contain a delivery system, such as biodegradable microspheres as disclosed, for example, in U.S. Pat. Nos. 4,897,268 and 5,075,109. Pharmaceutical compositions and vaccines within the scope of the present invention may also contain other compounds, including one or more separate polypeptides.

Alternatively, a vaccine may contain DNA encoding one or more of the polypeptides as described above, such that the polypeptide is generated in situ. In such vaccines, the DNA may be present within any of a variety of delivery systems known to those of ordinary skill in the art, including nucleic acid expression systems, bacteria and viral expression systems. Appropriate nucleic acid expression systems contain the necessary DNA sequences for expression in the patient (such as a suitable promoter and terminating signal). Bacterial delivery systems involve the administration of a bacterium (such as Bacillus-Calmette-Guerrin) that expresses an immunogenic portion of the polypeptide on its cell surface. In a preferred embodiment, the DNA may be introduced using a viral expression system (e.g., vaccinia or other pox virus, retrovirus, or adenovirus), which may involve the use of a non-pathogenic (defective), replication competent virus. Techniques for incorporating DNA into such expression systems are well known to those of ordinary skill in the art. The DNA may also be “naked,” as described, for example, in Ulmer et al., Science 259:1745-1749 (1993), and reviewed by Cohen, Science 259:1691-1692 (1993). The uptake of naked DNA may be increased by coating the DNA onto biodegradable beads, which are efficiently transported into the cells.

While any suitable carrier known to those of ordinary skill in the art may be employed in the pharmaceutical compositions of this invention, the type of carrier will vary depending on the mode of administration. For parenteral administration, such as subcutaneous injection, the carrier preferably comprises water, saline, alcohol, a fat, a wax or a buffer. For oral administration, any of the above carriers or a solid carrier, such as mannitol, lactose, starch, magnesium stearate, sodium saccharine, talcum, cellulose, glucose, sucrose, and magnesium carbonate, may be employed. Biodegradable microspheres (e.g., polylactate polyglycolate) may also be employed as carriers for the pharmaceutical compositions of this invention.

Any of a variety of adjuvants may be employed in the vaccines of this invention to nonspecifically enhance the immune response. Most adjuvants contain a substance designed to protect the antigen from rapid catabolism, such as aluminum hydroxide or mineral oil, and a stimulator of immune responses, such as lipid A, Bortadella pertussis or Mycobacterium tuberculosis derived proteins. Suitable adjuvants are commercially available as, for example, Freund's Incomplete Adjuvant and Complete Adjuvant (Difco Laboratories, Detroit, Mich.), Merck Adjuvant 65 (Merck and Company, Inc., Rahway, N.J.), alum, biodegradable microspheres, monophosphoryl lipid A and quil A. Cytokines, such as GM-CSF or interleukin-2, -7, or -12, may also be used as adjuvants.

The above pharmaceutical compositions and vaccines may be used, for example, for the therapy of breast cancer in a patient. As used herein, a “patient” refers to any warm-blooded animal, preferably a human. A patient may or may not be afflicted with breast cancer. Accordingly, the above pharmaceutical compositions and vaccines may be used to prevent the development of breast cancer or to treat a patient afflicted with breast cancer. In a preferred embodiment, the compounds are administered either prior to or following surgical removal of primary tumors and/or treatment by administration of radiotherapy and conventional chemotherapeutic drugs. To prevent or slow the development of breast cancer, a pharmaceutical composition or vaccine comprising one or more polypeptides as described herein may be administered to a patient. Alternatively, naked DNA or plasmid or viral vector encoding the polypeptide may be administered. For treating a patient with breast cancer, the pharmaceutical composition or vaccine may comprise one or more polypeptides, antibodies or polynucleotides complementary to DNA encoding a polypeptide as described herein (e.g., antisense RNA or antisense deoxyribonucleotide oligonucleotides).

Routes and frequency of administration, as well as dosage, will vary from individual to individual. In general, the pharmaceutical compositions and vaccines may be administered by injection (e.g., intracutaneous, intramuscular, intravenous or subcutaneous), intranasally (e.g., by aspiration) or orally. Between 1 and 10 doses may be administered for a 52-week period. Preferably, 6 doses are administered, at intervals of 1 month, and booster vaccinations may be given periodically thereafter. Alternate protocols may be appropriate for individual patients. A suitable dose is an amount of a compound that, when administered as described above, is capable of promoting an anti-tumor immune response. Such response can be monitored by measuring the anti-tumor antibodies in a patient or by vaccine-dependent generation of cytolytic effector cells capable of killing the patient's tumor cells in vitro. Such vaccines should also be capable of causing an immune response that leads to an improved clinical outcome (e.g., more frequent remissions, complete or partial or longer disease-free survival) in vaccinated patients as compared to non-vaccinated patients. In general, for pharmaceutical compositions and vaccines comprising one or more polypeptides, the amount of each polypeptide present in a dose ranges from about 100 μg to 5 mg. Suitable dose sizes will vary with the size of the patient, but will typically range from about 0.1 mL to about 5 mL.

Polypeptides disclosed herein may also be employed in adoptive immunotherapy for the treatment of cancer. Adoptive immunotherapy may be broadly classified into either active or passive immunotherapy. In active immunotherapy, treatment relies on the in vivo stimulation of the endogenous host immune system to react against tumors with the administration of immune response-modifying agents (for example, tumor vaccines, bacterial adjuvants, and/or cytokines).

In passive immunotherapy, treatment involves the delivery of biologic reagents with established tumor-immune reactivity (such as effector cells or antibodies) that can directly or indirectly mediate antitumor effects and does not necessarily depend on an intact host immune system. Examples of effector cells include T lymphocytes (for example, CD8+ cytotoxic T-lymphocyte, CD4+ T-helper, tumor-infiltrating lymphocytes), killer cells (Natural Killer cells, lymphokine-activated killer cells), B cells, or antigen presenting cells (such as dendritic cells and macrophages) expressing the disclosed antigens. The polypeptides disclosed herein may also be used to generate antibodies or anti-idiotypic antibodies (as in U.S. Pat. No. 4,918,164), for passive immunotherapy.

The predominant method of procuring adequate numbers of T-cells for adoptive immunotherapy is to grow immune T-cells in vitro. Culture conditions for expanding single antigen-specific T-cells to several billion in number with retention of antigen recognition in vivo are well known in the art. These in vitro culture conditions typically utilize intermittent stimulation with antigen, often in the presence of cytokines, such as IL-2, and non-dividing feeder cells. As noted above, the immunoreactive polypeptides described herein may be used to rapidly expand antigen-specific T cell cultures in order to generate sufficient number of cells for immunotherapy. In particular, antigen-presenting cells, such as dendritic, macrophage or B-cells, may be pulsed with immunoreactive polypeptides or transfected with a polynucleotide sequence(s), using standard techniques well known in the art. For cultured T-cells to be effective in therapy, the cultured T-cells must be able to grow and distribute widely and to survive long term in vivo. Studies have demonstrated that cultured T-cells can be induced to grow in vivo and to survive long term in substantial numbers by repeated stimulation with antigen supplemented with IL-2 (see, for example, Cheever et al. Ibid ).

The polypeptides disclosed herein may also be employed to generate and/or isolate tumor-reactive T-cells, which can then be administered to the patient. In one technique, antigen-specific T-cell lines may be generated by in vivo immunization with short peptides corresponding to immunogenic portions of the disclosed polypeptides. The resulting antigen specific CD8+ CTL clones may be isolated from the patient, expanded using standard tissue culture techniques, and returned to the patient.

Alternatively, peptides corresponding to immunogenic portions of the polypeptides may be employed to generate tumor reactive T cell subsets by selective in vitro stimulation and expansion of autologous T cells to provide antigen-specific T cells which may be subsequently transferred to the patient as described, for example, by Chang et al. ( Crit. Rev. Oncol. Hematol., 22(3), 213, 1996).

In another embodiment, syngeneic or autologous dendritic cells may be pulsed with peptides corresponding to at least an immunogenic portion of a polypeptide disclosed herein. The resulting antigen-specific dendritic cells may either be transferred into a patient, or employed to stimulate T cells to provide antigen-specific T cells which may, in turn, be administered to a patient. The use of peptide-pulsed dendritic cells to generate antigen-specific T cells and the subsequent use of such antigen-specific T cells to eradicate tumors in a murine model has been demonstrated by Cheever et al. (“Therapy With Cultured T Cells: Principles Revisited,” Immunological Reviews, 157:177, 1997).

Additionally vectors expressing the disclosed polynucleotides may be introduced into stem cells taken from the patient and clonally propagated in vitro for autologous transplant back into the same patient. In one embodiment, cells of the immune system, such as T cells, may be isolated from the peripheral blood of a patient, using a commercially available cell separation system, such as CellPro Incorporated's (Bothell, Wash.) CEPRATE™ system (see U.S. Pat. No. 5,240,856; U.S. Pat. No. 5,215,926; WO 89/06280; WO 91/16116 and WO 92/07243). The separated cells are stimulated with one or more of the immunoreactive polypeptides contained within a delivery vehicle, such as a microsphere, to provide antigen-specific T cells. The population of tumor antigen-specific T cells is then expanded using standard techniques and the cells are administered back to the patient.

The following Examples are offered by way of illustration and not by way of limitation.

EXAMPLES

Example 1

Preparation of Breast Tumor-Specific cDNAs Using Differential Display RT-PCR

This Example illustrates the preparation of cDNA molecules encoding breast tumor-specific polypeptides using a differential display screen.

A. Preparation of B18Ag1 cDNA and Characterization of mRNA Expression

Tissue samples were prepared from breast tumor and normal tissue of a patient with breast cancer that was confirmed by pathology after removal from the patient. Normal RNA and tumor RNA was extracted from the samples and mRNA was isolated and converted into cDNA using a (dT) 12 AG (SEQ ID NO:130) anchored 3′ primer. Differential display PCR was then executed using a randomly chosen primer (CTTCAACCTC) (SEQ ID NO: 103). Amplification conditions were standard buffer containing 1.5 mM MgCl 2 , 20 pmol of primer, 500 pmol dNTP, and 1 unit of Taq DNA polymerase (Perkin-Elmer, Branchburg, N.J.). Forty cycles of amplification were performed using 94° C. denaturation for 30 seconds, 42° C. annealing for 1 minute, and 72° C. extension for 30 seconds. An RNA fingerprint containing 76 amplified products was obtained. Although the RNA fingerprint of breast tumor tissue was over 98% identical to that of the normal breast tissue, a band was repeatedly observed to be specific to the RNA fingerprint pattern of the tumor. This band was cut out of a silver stained gel, subcloned into the T-vector (Novagen, Madison, Wis.) and sequenced.

The sequence of the cDNA, referred to as B18Ag1, is provided in SEQ ID NO:1. A database search of GENBANK and EMBL revealed that the B18Ag1 fragment initially cloned is 77% identical to the endogenous human retroviral element S71, which is a truncated retroviral element homologous to the Simian Sarcoma Virus (SSV). S71 contains an incomplete gag gene, a portion of the pol gene and an LTR-like structure at the 3′ terminus (see Werner et al., Virology 174:225-238 (1990)). B18Ag1 is also 64% identical to SSV in the region corresponding to the P30 (gag) locus. B18Ag1 contains three separate and incomplete reading frames covering a region which shares considerable homology to a wide variety of gag proteins of retroviruses which infect mammals. In addition, the homology to S71 is not just within the gag gene, but spans several kb of sequence including an LTR.

B18Ag1-specific PCR primers were synthesized using computer analysis guidelines. RT-PCR amplification (94° C., 30 seconds; 60° C.→42° C., 30 seconds; 72° C., 30 seconds for 40 cycles) confirmed that B18Ag1 represents an actual mRNA sequence present at relatively high levels in the patient's breast tumor tissue. The primers used in amplification were B18Ag1-1 (CTG CCT GAG CCA CAA ATG) (SEQ ID NO:128) and B18Ag1-4 (CCG GAG GAG GAA GCT AGA GGA ATA) (SEQ ID NO: 129) at a 3.5 mM magnesium concentration and a pH of 8.5, and B18Ag1-2 (ATG GCT ATT TTC GGG GCC TGA CA) (SEQ ID NO:126) and B18Ag1-3 (CCG GTA TCT CCT CGT GGG TAT T) (SEQ ID NO:127) at 2 mM magnesium at pH 9.5. The same experiments showed exceedingly low to nonexistent levels of expression in this patient's normal breast tissue (see FIG. 1 ). RT-PCR experiments were then used to show that B18Ag1 mRNA is present in nine other breast tumor samples (from Brazilian and American patients) but absent in, or at exceedingly low levels in, the normal breast tissue corresponding to each cancer patient. RT-PCR analysis has also shown that the B18Ag1 transcript is not present in various normal tissues (including lymph node, myocardium and liver) and present at relatively low levels in PBMC and lung tissue. The presence of B18Ag1 mRNA in breast tumor samples, and its absence from normal breast tissue, has been confirmed by Northern blot analysis, as shown in FIG. 2 .

The differential expression of B18Ag1 in breast tumor tissue was also confirmed by RNase protection assays. FIG. 3 shows the level of B18Ag1 mRNA in various tissue types as determined in four different RNase protection assays. Lanes 1-12 represent various normal breast tissue samples, lanes 13-25 represent various breast tumor samples; lanes 26-27 represent normal prostate samples; lanes 28-29 represent prostate tumor samples; lanes 30-32 represent colon tumor samples; lane 33 represents normal aorta; lane 34 represents normal small intestine; lane 35 represents normal skin, lane 36 represents normal lymph node; lane 37 represents normal ovary; lane 38 represents normal liver; lane 39 represents normal skeletal muscle; lane 40 represents a first normal stomach sample, lane 41 represents a second normal stomach sample; lane 42 represents a normal lung; lane 43 represents normal kidney; and lane 44 represents normal pancreas. Interexperimental comparison was facilitated by including a positive control RNA of known β-actin message abundance in each assay and normalizing the results of the different assays with respect to this positive control.

RT-PCR and Southern Blot analysis has shown the B18Ag1 locus to be present in human genomic DNA as a single copy endogenous retroviral element. A genomic clone of approximately 12-18 kb was isolated using the initial B18Ag1 sequence as a probe. Four additional subclones were also isolated by XbaI digestion. Additional retroviral sequences obtained from the ends of the XbaI digests of these clones (located as shown in FIG. 4 ) are shown as SEQ ID NO:3-SEQ ID NO:10, where SEQ ID NO:3 shows the location of the sequence labeled 10 in FIG. 4 , SEQ ID NO:4 shows the location of the sequence labeled 11-29, SEQ ID NO:5 shows the location of the sequence labeled 3, SEQ ID NO:6 shows the location of the sequence labeled 6, SEQ ID NO:7 shows the location of the sequence labeled 12, SEQ ID NO:8 shows the location of the sequence labeled 13, SEQ ID NO:9 shows the location of the sequence labeled 14 and SEQ ID NO:10 shows the location of the sequence labeled 11-22.

Subsequent studies demonstrated that the 12-18 kb genomic clone contains a retroviral element of about 7.75 kb, as shown in FIGS. 5A and 5B . The sequence of this retroviral element is shown in SEQ ID NO: 141. The numbered line at the top of FIG. 5A represents the sense strand sequence of the retroviral genomic clone. The box below this line shows the position of selected restriction sites. The arrows depict the different overlapping clones used to sequence the retroviral element. The direction of the arrow shows whether the single-pass subclone sequence corresponded to the sense or anti-sense strand. FIG. 5B is a schematic diagram of the retroviral element containing B18Ag1 depicting the organization of viral genes within the element. The open boxes correspond to predicted reading frames, starting with a methionine, found throughout the element. Each of the six likely reading frames is shown, as indicated to the left of the boxes, with frames 1-3 corresponding to those found on the sense strand.

Using the cDNA of SEQ ID NO: 1 as a probe, a longer cDNA was obtained (SEQ ID NO:227) which contains minor nucleotide differences (less than 1%) compared to the genomic sequence shown in SEQ ID NO: 141

B. Preparation of cDNA Molecules Encoding Other Breast Tumor-Specific Polypeptides

Normal RNA and tumor RNA was prepared and mRNA was isolated and converted into cDNA using a (dT) 12 AG anchored 3′ primer, as described above. Differential display PCR was then executed using the randomly chosen primers of SEQ ID NO: 87-125. Amplification conditions were as noted above, and bands observed to be specific to the RNA fingerprint pattern of the tumor were cut out of a silver stained gel, subcloned into either the T-vector (Novagen, Madison, Wis.) or the pCRII vector (Invitrogen, San Diego, Calif.) and sequenced. The sequences are provided in SEQ ID NO: 11-SEQ ID NO:86. Of the 79 sequences isolated, 67 were found to be novel (SEQ ID NO: 11-26 and 28-77) (see also FIGS. 6 - 20 ).

An extended DNA sequence (SEQ ID NO: 290) for the antigen B15Ag1 (originally identified partial sequence provided in SEQ ID NO: 27) was obtained in further studies. Comparison of the sequence of SEQ ID NO: 290 with those in the gene bank as described above, revealed homology to the known human β-A activin gene. Further studies led to the isolation of the full-length cDNA sequence for the antigen B21GT2 (originally identified partial cDNA sequence provided in SEQ ID NO: 56). The full-length sequence is provided in SEQ ID NO: 307.

Subsequent studies identified an additional 146 sequences (SEQ ID NOS:142-289), of which 115 appeared to be novel (SEQ ID NOS:142, 143, 146-152, 154-166, 168-176, 178-192, 194-198, 200-204, 206, 207, 209-214, 216, 218, 219, 221-240, 243-245, 247, 250, 251, 253, 255, 257-266, 268, 269, 271-273, 275, 276, 278, 280, 281, 284, 288 and 291). To the best of the inventors' knowledge none of the previously identified sequences have heretofore been shown to be expressed at a greater level in human breast tumor tissue than in normal breast tissue.

In further studies, several different splice forms of the antigen B11Ag1 (also referred to as B305D) were isolated, with each of the various splice forms containing slightly different versions of the B11Ag1 coding frame. Splice junction sequences define individual exons which, in various patterns and arrangements, make up the various splice forms. Primers were designed to examine the expression pattern of each of the exons using RT-PCR as described below. Each exon was found to show the same expression pattern as the original B11Ag1 clone, with expression being breast tumor-, normal prostate- and normal testis-specific. The determined cDNA sequences for the isolated protein coding exons are provided in SEQ ID NO: 292-298, respectively. The predicted amino acid sequences corresponding to the sequences of SEQ ID NO: 292 and 298 are provided in SEQ ID NO: 299 and 300. Additional studies using rapid amplification of cDNA ends (RACE), a 5′ specific primer to one of the splice forms of B11Ag1 provided above and a breast adenocarcinoma, led to the isolation of three additional, related, splice forms referred to as isoforms B11C-15, B11C-8 and B11C-9,16. The determined cDNA sequences for these isoforms are provided in SEQ ID NO: 301-303, with the corresponding predicted amino acid sequences being provided in SEQ ID NO: 304-306.

In subsequent studies on B305D isoform A (cDNA sequence provided in SEQ ID NO: 292), the cDNA sequence (provided in SEQ ID NO: 313) was found to contain an additional guanine residue at position 884, leading to a frameshift in the open reading frame. The determined DNA sequence of this ORF is provided in SEQ ID NO: 314. This frameshift generates a protein sequence (provided in SEQ ID NO: 315) of 293 amino acids that contains the C-terminal domain common to the other isoforms of B305D but that differs in the N-terminal region.

Example 2

Preparation of B18AG1 DNA from Human Genomic DNA

This Example illustrates the preparation of B18Ag1 DNA by amplification from human genomic DNA.

B18Ag1 DNA may be prepared from 250 ng human genomic DNA using 20 pmol of B18Ag1 specific primers, 500 pmol dNTPS and 1 unit of Taq DNA polymerase (Perkin Elmer, Branchburg, N.J.) using the following amplification parameters: 94° C. for 30 seconds denaturing, 30 seconds 60° C. to 42° C. touchdown annealing in 2° C. increments every two cycles and 72° C. extension for 30 seconds. The last increment (a 42° C. annealing temperature) should cycle 25 times. Primers were selected using computer analysis. Primers synthesized were B18Ag1-1, B18Ag1-2, B18Ag1-3, and B18Ag1-4. Primer pairs that may be used are 1+3, 1+4, 2+3, and 2+4.

Following gel electrophoresis, the band corresponding to B18Ag1 DNA may be excised and cloned into a suitable vector.

Example 3

Preparation of B18AG1 DNA from Breast Tumor cDNA

This Example illustrates the preparation of B18Ag1 DNA by amplification from human breast tumor cDNA.

First strand cDNA is synthesized from RNA prepared from human breast tumor tissue in a reaction mixture containing 500 ng poly A+RNA, 200 pmol of the primer (T) 12 AG (i.e., TTT TTT TTT TTT AG) (SEQ ID NO: 130), IX first strand reverse transcriptase buffer, 6.7 mM DTT, 500 mmol dNTPs, and 1 unit AMV or MMLV reverse transcriptase (from any supplier, such as Gibco-BRL (Grand Island, N.Y.)) in a final volume of 30 μl. After first strand synthesis, the cDNA is diluted approximately 25 fold and 1 μl is used for amplification as described in Example 2. While some primer pairs can result in a heterogeneous population of transcripts, the primers B18Ag1-2 (5′ATG GCT ATT TTC GGG GGC TGA CA) (SEQ ID NO: 126) and B18Ag1-3 (5′CCG GTA TCT CCT CGT GGG TAT T) (SEQ ID NO: 127) yield a single 151 bp amplification product.

Example 4

Identification of B-Cell and T-Cell Epitopes of B18AG1

This Example illustrates the identification of B18Ag1 epitopes.

The B18Ag1 sequence can be screened using a variety of computer algorithms. To determine B-cell epitopes, the sequence can be screened for hydrophobicity and hydrophilicity values using the method of Hopp, Prog. Clin. Biol. Res. 172B:367-77 (1985) or, alternatively, Cease et al., J. Exp. Med 164:1779-84 (1986) or Spouge et al., J. Immunol. 138:204-12 (1987). Additional Class II MHC (antibody or B-cell) epitopes can be predicted using programs such as AMPHI (e.g., Margalit et al., J. Immunol. 138:2213 (1987)) or the methods of Rothbard and Taylor (e.g., EMBO J. 7:93(1988)).

Once peptides (15-20 amino acids long) are identified using these techniques, individual peptides can be synthesized using automated peptide synthesis equipment (available from manufacturers such as Perkin Elmer/Applied Biosystems Division, Foster City, Calif.) and techniques such as Merrifield synthesis. Following synthesis, the peptides can used to screen sera harvested from either normal or breast cancer patients to determine whether patients with breast cancer possess antibodies reactive with the peptides. Presence of such antibodies in breast cancer patient would confirm the immunogenicity of the specific B-cell epitope in question. The peptides can also be tested for their ability to generate a serologic or humoral immune in animals (mice, rats, rabbits, chimps etc.) following immunization in vivo. Generation of a peptide-specific antiserum following such immunization further confirms the immunogenicity of the specific B-cell epitope in question.

To identify T-cell epitopes, the B18Ag1 sequence can be screened using different computer algorithms which are useful in identifying 8-10 amino acid motifs within the B18Ag1 sequence which are capable of binding to HLA Class I MHC molecules. (see, e.g., Rammensee et al., Immunogenetics 41:178-228 (1995)). Following synthesis such peptides can be tested for their ability to bind to class I MHC using standard binding assays (e.g., Sette et al., J. Immunol 153:5586-92 (1994)) and more importantly can be tested for their ability to generate antigen reactive cytotoxic T-cells following in vitro stimulation of patient or normal peripheral mononuclear cells using, for example, the methods of Bakker et al., Cancer Res. 55:5330-34 (1995); Visseren et al., J. Immunol. 154:3991-98 (1995); Kawakami et al., J. Immunol. 154:3961-68 (1995); and Kast et al., J. Immunol. 152:3904-12 (1994). Successful in vitro generation of T-cells capable of killing autologous (bearing the same Class I MHC molecules) tumor cells following in vitro peptide stimulation further confirms the immunogenicity of the B18Ag1 antigen. Furthermore, such peptides may be used to generate murine peptide and B18Ag1 reactive cytotoxic T-cells following in vivo immunization in mice rendered transgenic for expression of a particular human MHC Class I haplotype (Vitiello et al., J. Exp. Med 173:1007-15 (1991).

A representative list of predicted B18Ag1 B-cell and T-cell epitopes, broken down according to predicted HLA Class I MHC binding antigen, is shown below:

Predicted Th Motifs (B-cell epitopes) (SEQ ID NOS.: 131-133)

SSGGRTFDDFHRYLLVGI

QGAAQKPINLSKXIEVVQGHDE

SPGVFLEHLQEAYRIYTPFDLSA

Predicted HLA A2.1 Motifs (T-cell epitopes) (SEQ ID NOS.: 134-140)

YLLVGIQGA

GAAQKPINL

NLSKXIEVV

EVVQGHDES

HLQEAYRIY

NLAFVAQAA

FVAQAAPDS

Example 5

Identification of T-Cell Epitopes of B11AG1

This Example illustrates the identification of B11Ag1 (also referred to as B305D) epitopes. Four peptides, referred to as B11-8, B11-1, B11-5 and B11-12 (SEQ ID NO: 309-312, respectfully) were derived from the B11Ag1 gene.

Human CD8 T cells were primed in vitro to the peptide B11-8 using dendritic cells according to the protocol of Van Tsai et al. ( Critical Reviews in Immunology 18:65-75, 1998). The resulting CD8 T cell cultures were tested for their ability to recognize the B11-8 peptide or a negative control peptide, presented by the B-LCL line, JY. Briefly, T cells were incubated with autologous monocytes in the presence of 10 ug/ml peptide, 10 ng/ml IL-7 and 10 ug/ml IL-2, and assayed for their ability to specifically lyse target cells in a standard 51-Cr release assay. As shown in FIG. 22 , the bulk culture line demonstrated strong recognition of the B11-8 peptide with weaker recognition of the peptide B11-1.

A clone from this CTL line was isolated following rapid expansion using the monoclonal antibody OKT3 and human IL-2. As shown in FIG. 23 , this clone (referred to as A1), in addition to being able to recognize specific peptide, recognized JY LCL transduced with the B11Ag1 gene. This data demonstrates that B11-8 is a naturally processed epitope of the B11Ag1 gene. In addition these T cells were further found to recognize and lyse, in an HLA-A2 restricted manner, an established tumor cell line naturally expressing B11Ag1 (FIG. 24 ). The T cells strongly recognize a lung adenocarcinoma (LT-140-22) naturally expressing B11Ag1 transduced with HLA-A2, as well as an A2+ breast carcinoma (CAMA-1) transduced with B11Ag1, but not untransduced lines or another negative tumor line (SW620).

These data clearly demonstrate that these human T cells recognize not only B11-specific peptides but also transduced cells, as well as naturally expressing tumor lines.

CTL lines raised against the antigens B11-5 and B11-12, using the procedures described above, were found to recognize corresponding peptide-coated targets.

Example 6

Characterization of Breast Tumor Genes Discovered By Differential Display PCR

The specificity and sensitivity of the breast tumor genes discovered by differential display PCR were determined using RT-PCR. This procedure enabled the rapid evaluation of breast tumor gene mRNA expression semiquantitatively without using large amounts of RNA. Using gene specific primers, mRNA expression levels in a variety of tissues were examined, including 8 breast tumors, 5 normal breasts, 2 prostate tumors, 2 colon tumors, 1 lung tumor, and 14 other normal adult human tissues, including normal prostate, colon, kidney, liver, lung, ovary, pancreas, skeletal muscle, skin, stomach and testes.

To ensure the semiquantitative nature of the RT-PCR, β-actin was used as internal control for each of the tissues examined. Serial dilutions of the first strand cDNAs were prepared and RT-PCR assays performed using β-actin specific primers. A dilution was then selected that enabled the linear range amplification of β -actin template, and which was sensitive enough to reflect the difference in the initial copy number. Using this condition, the β-actin levels were determined for each reverse transcription reaction from each tissue. DNA contamination was minimized by DNase treatment and by assuring a negative result when using first strand cDNA that was prepared without adding reverse transcriptase.

Using gene specific primers, the mRNA expression levels were determined in a variety of tissues. To date, 38 genes have been successfully examined by RT-PCR, five of which exhibit good specificity and sensitivity for breast tumors (B15AG-1, B31GA1b, B38GA2a, B11A1a and B18AG1a). FIGS. 21A and 21B depict the results for three of these genes: B15AG-1 (SEQ ID NO:27), B31GA1b (SEQ ID NO:148) and B38GA2a (SEQ ID NO. 157). Table I summarizes the expression level of all the genes tested in normal breast tissue and breast tumors, and also in other tissues.

TABLE I
Percentage of Breast Cancer Antigens
that are Expressed in Various Tissues
Breast Tissues	Over-expressed in Breast Tumors	84%
	Equally Expressed in Normals and Tumor	16%
Other Tissues	Over-expressed in Breast Tumors but	9%
	not in any Normal Tissues
	Over-expressed in Breast Tumors but	30%
	Expressed in Some Normal Tissues
	Over-expressed in Breast Tumors but	61%
	Equally Expressed in All Other Tissues

From the foregoing, it will be appreciated that, although specific embodiments of the invention have been described herein for the purpose of illustration, various modifications may be made without deviating from the spirit and scope of the invention.

315 1 363 DNA Homo sapien 1ttagagaccc aattgggacc taattgggac ccaaatttct caagtggagg gagaactttt 60gacgatttcc accggtatct cctcgtgggt attcagggag ctgcccagaa acctataaac 120ttgtctaagg cgattgaagt cgtccagggg catgatgagt caccaggagt gtttttagag 180cacctccagg aggcttatcg gatttacacc ccttttgacc tggcagcccc cgaaaatagc 240catgctctta atttggcatt tgtggctcag gcagccccag atagtaaaag gaaactccaa 300aaactagagg gattttgctg gaatgaatac cagtcagctt ttagagatag cctaaaaggt 360ttt 363 2 121 PRT Homo sapien 2Leu Glu Thr Gln Leu Gly Pro Asn Trp Asp Pro Asn Phe Ser Ser Gly 1 5 10 15Gly Arg Thr Phe Asp Asp Phe His Arg Tyr Leu Leu Val Gly Ile Gln 20 25 30Gly Ala Ala Gln Lys Pro Ile Asn Leu Ser Lys Ala Ile Glu Val Val 35 40 45Gln Gly His Asp Glu Ser Pro Gly Val Phe Leu Glu His Leu Gln Glu 50 55 60Ala Tyr Arg Ile Tyr Thr Pro Phe Asp Leu Ala Ala Pro Glu Asn Ser65 70 75 80His Ala Leu Asn Leu Ala Phe Val Ala Gln Ala Ala Pro Asp Ser Lys 85 90 95Arg Lys Leu Gln Lys Leu Glu Gly Phe Cys Trp Asn Glu Tyr Gln Ser 100 105 110Ala Phe Arg Asp Ser Leu Lys Gly Phe 115 120 3 1080 DNA Homo sapien misc_feature (1)...(1080) n = A,T,C or G 3tcttagaatc ttcatacccc gaactcttgg gaaaacttta atcagtcacc tacagtctac 60cacccattta ggaggagcaa agctacctca gctcctccgg agccgtttta agatccccca 120tcttcaaagc ctaacagatc aagcagctct ccggtgcaca acctgcgccc aggtaaatgc 180caaaaaaggt cctaaaccca gcccaggcca ccgtctccaa gaaaactcac caggagaaaa 240gtgggaaatt gactttacag aagtaaaacc acaccgggct gggtacaaat accttctagt 300actggtagac accttctctg gatggactga agcatttgct accaaaaacg aaactgtcaa 360tatggtagtt aagtttttac tcaatgaaat catccctcga cgtgggctgc ctgttgccat 420agggtctgat aatggaacgg ccttcgcctt gtctatagtt taatcagtca gtaaggcgtt 480aaacattcaa tggaagctcc attgtgccta tcgacccaga gctctgggca agtagaacgc 540atgaactgca ccctaaaaaa acactcttac aaaattaatc ttaaaaaccg gtgttaattg 600tgttagtctc cttcccttag ccctacttag agttaaggtg caccccttac tgggctgggt 660tctttacctt ttgaaatcat ntttnggaag gggctgccta tctttnctta actaaaaaan 720gcccatttgg caaaaatttc ncaactaatt tntacgtncc tacgtctccc caacaggtan 780aaaaatctnc tgcccttttc aaggaaccat cccatccatt cctnaacaaa aggcctgccn 840ttcttccccc agttaactnt tttttnttaa aattcccaaa aaangaaccn cctgctggaa 900aaacnccccc ctccaanccc cggccnaagn ggaaggttcc cttgaatccc ncccccncna 960anggcccgga accnttaaan tngttccngg gggtnnggcc taaaagnccn atttggtaaa 1020cctanaaatt ttttcttttn taaaaaccac nntttnnttt ttcttaaaca aaaccctntt 1080 4 1087 DNA Homo sapien misc_feature (1)...(1087) n = A,T,C or G 4tctagagctg cgcctggatc ccgccacagt gaggagacct gaagaccaga gaaaacacag 60caagtaggcc ctttaaacta ctcacctgtg ttgtcttcta atttattctg ttttattttg 120tttccatcat tttaaggggt taaaatcatc ttgttcagac ctcagcatat aaaatgaccc 180atctgtagac ctcaggctcc aaccataccc caagagttgt ctggttttgt ttaaattact 240gccaggtttc agctgcagat atccctggaa ggaatattcc agattccctg agtagtttcc 300aggttaaaat cctataggct tcttctgttt tgaggaagag ttcctgtcag agaaaaacat 360gattttggat ttttaacttt aatgcttgtg aaacgctata aaaaaaattt tctaccccta 420gctttaaagt actgttagtg agaaattaaa attccttcag gaggattaaa ctgccatttc 480agttacccta attccaaatg ttttggtggt tagaatcttc tttaatgttc ttgaagaagt 540gttttatatt ttcccatcna gataaattct ctcncncctt nnttttntnt ctnntttttt 600aaaacggant cttgctccgt tgtccangct gggaattttn ttttggccaa tctccgctnc 660cttgcaanaa tnctgcntcc caaaattacc ncctttttcc cacctccacc ccnnggaatt 720acctggaatt anaggccccc nccccccccc cggctaattt gtttttgttt ttagtaaaaa 780acgggtttcc tgttttagtt aggatggccc anntctgacc ccntnatcnt ccccctcngc 840cctcnaatnt tnggnntang gcttaccccc cccngnngtt tttcctccat tnaaattttc 900tntggantct tgaatnncgg gttttccctt ttaaaccnat tttttttttn nnncccccan 960ttttncctcc cccntntnta angggggttt cccaanccgg gtccnccccc angtccccaa 1020tttttctccc cccccctctt ttttctttnc cccaaaantc ctatcttttc ctnnaaatat 1080cnantnt 1087 5 1010 DNA Homo sapien misc_feature (1)...(1010) n = A,T,C or G 5tctagaccaa gaaatgggag gattttagag tgactgatga tttctctatc atctgcagtt 60agtaaacatt ctccacagtt tatgcaaaaa gtaacaaaac cactgcagat gacaaacact 120aggtaacaca catactatct cccaaatacc tacccacaag ctcaacaatt ttaaactgtt 180aggatcactg gctctaatca ccatgacatg aggtcaccac caaaccatca agcgctaaac 240agacagaatg tttccactcc tgatccactg tgtgggaaga agcaccgaac ttacccactg 300gggggcctgc ntcanaanaa aagcccatgc ccccgggtnt ncctttnaac cggaacgaat 360naacccacca tccccacanc tcctctgttc ntgggccctg catcttgtgg cctcntntnc 420tttnggggan acntggggaa ggtaccccat ttcnttgacc ccncnanaaa accccngtgg 480ccctttgccc tgattcncnt gggccttttc tcttttccct tttgggttgt ttaaattccc 540aatgtccccn gaaccctctc cntnctgccc aaaacctacc taaattnctc nctangnntt 600ttcttggtgt tncttttcaa aggtnacctt ncctgttcan ncccnacnaa aatttnttcc 660ntatnntggn cccnnaaaaa nnnatcnncc cnaattgccc gaattggttn ggtttttcct 720nctgggggaa accctttaaa tttccccctt ggccggcccc ccttttttcc cccctttnga 780aggcaggngg ttcttcccga acttccaatt ncaacagccn tgcccattgn tgaaaccctt 840ttcctaaaat taaaaaatan ccggttnngg nnggcctctt tcccctccng gngggnngng 900aaantcctta ccccnaaaaa ggttgcttag cccccngtcc ccactccccc nggaaaaatn 960aaccttttcn aaaaaaggaa tataantttn ccactccttn gttctcttcc 1010 6 950 DNA Homo sapien misc_feature (1)...(950) n = A,T,C or G 6tctagagctc gcggccgcga gctctaatac gactcactat agggcgtcga ctcgatctca 60gctcactgca atctctgccc ccggggtcat gcgattctcc tgcctcagcc ttccaagtag 120ctgggattac aggcgtgcaa caccacaccc ggctaatttt gtatttttaa tagagatggg 180gttttccctt gttggccann atggtctcna acccctgacc tcnngtgatc cccccncccn 240nganctcnna ctgctgggga tnnccgnnnn nnncctcccn ncncnnnnnn ncncnntccn 300tnntccttnc tcnnnnnnnn cnntcnntcc nncttctcnc cnnntnttnt cnncnnccnn 360cnnnccncnt ncccncnnnt tcncntncnn tntccnncnn nntcnncnnn cnnnncntnn 420ccnntacntc ntnnncnnnt ccntctntnn cctcnncnnt cnctncncnt tntctcctcn 480ntnnnnnnct ccnnnnntct cntcncnncn tncctcnntn nccncncccc ncctcncnnc 540ctnntttnnn cnncnnntcc ntnccnttcn nntccnntnn cnncntcncn nncnttnttc 600ccnccnnttc cttncncntn nnntntcnnn cncntcnntc ntttnctcct nnntcccnnc 660tcnnttcncc cnnntccncc ccccncctnt ctctcncccn nntnnntntn nnncntccnc 720tntcncnttc ntcnntncnt tnctntcnnc nncnntncnc tnccntntnt ctnnntcncn 780tcncntntcn ccntccnttn ctntctcctn tntccttccc ctcncctnct cnttcnccnc 840ccnntntntn tnncnccnnt nctnnncnnc cntcntttcn tctctnctnn nnntnncctc 900nncccntncc ctnntncnct nctnntaccn tnctnctccn tcttccttcc 950 7 1086 DNA Homo sapien misc_feature (1)...(1086) n = A,T,C or G 7tctagagctc gcggccgcga gctcaattaa ccctcactaa agggagtcga ctcgatcaga 60ctgttactgt gtctatgtag aaagaagtag acataagaga ttccattttg ttctgtacta 120agaaaaattc ttctgccttg agatgctgtt aatctgtaac cctagcccca accctgtgct 180cacagagaca tgtgctgtgt tgactcaagg ttcaatggat ttagggctat gctttgttaa 240aaaagtgctt gaagataata tgcttgttaa aagtcatcac cattctctaa tctcaagtac 300ccagggacac aatacactgc ggaaggccgc agggacctct gtctaggaaa gccaggtatt 360gtccaagatt tctccccatg tgatagcctg agatatggcc tcatgggaag ggtaagacct 420gactgtcccc cagcccgaca tcccccagcc cgacatcccc cagcccgaca cccgaaaagg 480gtctgtgctg aggaagatta ntaaaagagg aaggctcttt gcattgaagt aagaagaagg 540ctctgtctcc tgctcgtccc tgggcaataa aatgtcttgg tgttaaaccc gaatgtatgt 600tctacttact gagaatagga gaaaacatcc ttagggctgg aggtgagaca ccctggcggc 660atactgctct ttaatgcacg agatgtttgt ntaattgcca tccagggcca ncccctttcc 720ttaacttttt atganacaaa aactttgttc ncttttcctg cgaacctctc cccctattan 780cctattggcc tgcccatccc ctccccaaan ggtgaaaana tgttcntaaa tncgagggaa 840tccaaaacnt tttcccgttg gtcccctttc caaccccgtc cctgggccnn tttcctcccc 900aacntgtccc ggntccttcn ttcccncccc cttcccngan aaaaaacccc gtntganggn 960gccccctcaa attataacct ttccnaaaca aannggttcn aaggtggttt gnttccggtg 1020cggctggcct tgaggtcccc cctncacccc aatttggaan ccngtttttt ttattgcccn 1080ntcccc 1086 8 1177 DNA Homo sapien misc_feature (1)...(1177) n = A,T,C or G 8nccntttaga tgttgacaan ntaaacaagc ngctcaggca gctgaaaaaa gccactgata 60aagcatcctg gagtatcaga gtttactgtt agatcagcct catttgactt cccctcccac 120atggtgttta aatccagcta cactacttcc tgactcaaac tccactattc ctgttcatga 180ctgtcaggaa ctgttggaaa ctactgaaac tggccgacct gatcttcaaa atgtgcccct 240aggaaaggtg gatgccaccg tgttcacaga cagtaccncc ttcctcgaga agggactacg 300aggggccggt gcanctgtta ccaaggagac tnatgtgttg tgggctcagg ctttaccanc 360aaacacctca ncncnnaagg ctgaattgat cgccctcact caggctctcg gatggggtaa 420gggatattaa cgttaacact gacagcaggt acgcctttgc tactgtgcat gtacgtggag 480ccatctacca ggagcgtggg ctactcactc ggcaggtggc tgtnatccac tgtaaangga 540catcaaaagg aaaacnnggc tgttgcccgt ggtaaccana aanctgatcn ncagctcnaa 600gatgctgtgt tgactttcac tcncncctct taaacttgct gcccacantc tcctttccca 660accagatctg cctgacaatc cccatactca aaaaaaaaan aanactggcc ccgaacccna 720accaataaaa acggggangg tnggtnganc nncctgaccc aaaaataatg gatcccccgg 780gctgcaggaa ttcaattcan ccttatcnat acccccaacn nggngggggg ggccngtncc 840cattncccct ntattnattc tttnnccccc cccccggcnt cctttttnaa ctcgtgaaag 900ggaaaacctg ncttaccaan ttatcncctg gaccntcccc ttccncggtn gnttanaaaa 960aaaagcccnc antcccntcc naaatttgca cngaaaggna aggaatttaa cctttatttt 1020ttnntccttt antttgtnnn ccccctttta cccaggcgaa cngccatcnt ttaanaaaaa 1080aaanagaang tttatttttc cttngaacca tcccaatana aancacccgc nggggaacgg 1140ggnggnaggc cnctcacccc ctttntgtng gngggnc 1177 9 1146 DNA Homo sapien misc_feature (1)...(1146) n = A,T,C or G 9nccnnttnnt gatgttgtct ttttggcctc tctttggata ctttccctct cttcagaggt 60gaaaagggtc aaaaggagct gttgacagtc atcccaggtg ggccaatgtg tccagagtac 120agactccatc agtgaggtca aagcctgggg cttttcagag aagggaggat tatgggtttt 180ccaattatac aagtcagaag tagaaagaag ggacataaac caggaagggg gtggagcact 240catcacccag agggacttgt gcctctctca gtggtagtag aggggctact tcctcccacc 300acggttgcaa ccaagaggca atgggtgatg agcctacagg ggacatancc gaggagacat 360gggatgaccc taagggagta ggctggtttt aaggcggtgg gactgggtga gggaaactct 420cctcttcttc agagagaagc agtacagggc gagctgaacc ggctgaaggt cgaggcgaaa 480acacggtctg gctcaggaag accttggaag taaaattatg aatggtgcat gaatggagcc 540atggaagggg tgctcctgac caaactcagc cattgatcaa tgttagggaa actgatcagg 600gaagccggga atttcattaa caacccgcca cacagcttga acattgtgag gttcagtgac 660ccttcaaggg gccactccac tccaactttg gccattctac tttgcnaaat ttccaaaact 720tcctttttta aggccgaatc cntantccct naaaaacnaa aaaaaatctg cncctattct 780ggaaaaggcc cancccttac caggctggaa gaaattttnc cttttttttt tttttgaagg 840cntttnttaa attgaacctn aattcncccc cccaaaaaaa aacccnccng gggggcggat 900ttccaaaaac naattccctt accaaaaaac aaaaacccnc ccttnttccc ttccnccctn 960ttcttttaat tagggagaga tnaagccccc caatttccng gnctngatnn gtttcccccc 1020cccccatttt ccnaaacttt ttcccancna ggaanccncc ctttttttng gtcngattna 1080ncaaccttcc aaaccatttt tccnnaaaaa ntttgntngg ngggaaaaan acctnntttt 1140atagan 1146 10 545 DNA Homo sapien 10cttcattggg tacgggcccc ctcgaggtcg acggtatcga taagcttgat atcgaattcc 60tgcagcccgg gggatccact agttctagag tcaggaagaa ccaccaacct tcctgatttt 120tattggctct gagttctgag gccagttttc ttcttctgtt gagtatgcgg gattgtcagg 180cagatctggc tgtggaaagg agactgtggg cagcaagttt agaggcgtga ctgaaagtca 240cactgcatct tgagctgctg aatcagcttt ctggttacca cgggcaacag ccgtgttttc 300cttttgatgt cctttacagt ggattacagc cacctgctga ggtgagtagc ccacgctcct 360ggtagatggc tccacgtaca tgcacagtag caaaggcgta cctgctgtca gtgttaacgt 420taatatcctt accccatcgg agagcctgag tgagggcgat caattcagcc cttttgtgct 480gaggtgtttg ctggttaagc cctgaaccca caacacatct gtctccatgg taacagctgc 540accgg 545 11 196 DNA Homo sapien 11tctcctaggc tgggcacagt ggctcatacc tgtaatcctg accgtttcag aggctcaggt 60ggggggatcg cttgagccca agatttcaag actagtctgg gtaacatagt gagaccctat 120ctctacgaaa aaataaaaaa atgagcctgg tgtagtggca cacaccagct gaggagggag 180aatcgagcct aggaga 196 12 388 DNA Homo sapien misc_feature (1)...(388) n = A,T,C or G 12tctcctaggc ttgggggctc tgactagaaa ttcaaggaac ctgggattca agtccaactg 60tgacaccaac ttacactgtg gnctccaata aactgcttct ttcctattcc ctctctatta 120aataaaataa ggaaaacgat gtctgtgtat agccaagtca gntatcctaa aaggagatac 180taagtgacat taaatatcag aatgtaaaac ctgggaacca ggttcccagc ctgggattaa 240actgacagca agaagactga acagtactac tgtgaaaagc ccgaagnggc aatatgttca 300ctctaccgtt gaaggatggc tgggagaatg aatgctctgt cccccagtcc caagctcact 360tactatacct cctttatagc ctaggaga 388 13 337 DNA Homo sapien 13tagtagttgc ctataatcat gtttctcatt attttcacat tttattaacc aatttctgtt 60taccctgaaa aatatgaggg aaatatatga aacagggagg caatgttcag ataattgatc 120acaagatatg atttctacat cagatgctct ttcctttcct gtttatttcc tttttatttc 180ggttgtgggg tcgaatgtaa tagctttgtt tcaagagaga gttttggcag tttctgtagc 240ttctgacact gctcatgtct ccaggcatct atttgcactt taggaggtgt cgtgggagac 300tgagaggtct attttttcca tatttgggca actacta 337 14 571 DNA Homo sapien misc_feature (1)...(571) n = A,T,C or G 14tagtagttgc catacagtgc ctttccattt atttaacccc cacctgaacg gcataaactg 60agtgttcagc tggtgttttt tactgtaaac aataaggaga ctttgctctt catttaaacc 120aaaatcatat ttcatatttt acgctcgagg gtttttaccg gttccttttt acactcctta 180aaacagtttt taagtcgttt ggaacaagat attttttctt tcctggcagc ttttaacatt 240atagcaaatt tgtgtctggg ggactgctgg tcactgtttc tcacagttgc aaatcaaggc 300atttgcaacc aagaaaaaaa aatttttttg ttttatttga aactggaccg gataaacggt 360gtttggagcg gctgctgtat atagttttaa atggtttatt gcacctcctt aagttgcact 420tatgtggggg ggggnttttg natagaaagt ntttantcac anagtcacag ggacttttnt 480cttttggnna ctgagctaaa aagggctgnt tttcgggtgg gggcagatga aggctcacag 540gaggcctttc tcttagaggg gggaactnct a 571 15 548 DNA Homo sapien misc_feature (1)...(548) n = A,T,C or G 15tatatattta ataacttaaa tatattttga tcacccactg gggtgataag acaatagata 60taaaagtatt tccaaaaagc ataaaaccaa agtatcatac caaaccaaat tcatactgct 120tcccccaccc gcactgaaac ttcaccttct aactgtctac ctaaccaaat tctacccttc 180aagtctttgg tgcgtgctca ctactctttt tttttttttt tttnttttgg agatggagtc 240tggctgtgca gcccaggggt ggagtacaat ggcacaacct cagctcactg naacctccgc 300ctcccaggtt catgagattc tcctgnttca gccttcccag tagctgggac tacaggtgtg 360catcaccatg cctggntaat cttttttngt tttngggtag agatgggggt tttacatgtt 420ggccaggntg gtntcgaact cctgacctca agtgatccac ccacctcagg ctcccaaagt 480gctaggatta cagacatgag ccactgngcc cagncctggt gcatgctcac ttctctaggc 540aactacta 548 16 638 DNA Homo sapien misc_feature (1)...(638) n = A,T,C or G 16ttccgttatg cacatgcaga atattctatc ggtacttcag ctattactca ttttgatggc 60gcaatccgag cctatcctca agatgagtat ttagaaagaa ttgatttagc gatagaccaa 120gctggtaagc actctgacta cacgaaattg ttcagatgtg atggatttat gacagttgat 180ctttggaaga gattattaag tgattatttt aaagggaatc cattaattcc agaatatctt 240ggtttagctc aagatgatat agaaatagaa cagaaagaga ctacaaatga agatgtatca 300ccaactgata ttgaagagcc tatagtagaa aatgaattag ctgcatttat tagccttaca 360catagcgatt ttcctgatga atcttatatt cagccatcga catagcatta cctgatgggc 420aaccttacga ataatagaaa ctgggtgcgg ggctattgat gaattcatcc ncagtaaatt 480tggatatnac aaaatataac tcgattgcat ttggatgatg gaatactaaa tctggcaaaa 540gtaactttgg agctactagt aacctctctt tttgagatgc aaaattttct tttagggttt 600cttattctct actttacgga tattggagca taacggga 638 17 286 DNA Homo sapien 17actgatggat gtcgccggag gcgaggggcc ttatctgatg ctcggctgcc tgttcgtgat 60gtgcgcggcg attgggctgt ttatctcaaa caccgccacg gcggtgctga tggcgcctat 120tgccttagcg gcggcgaagt caatgggcgt ctcaccctat ccttttgcca tggtggtggc 180gatggcggct tcggcggcgt ttatgacccc ggtctcctcg ccggttaaca ccctggtgct 240tggccctggc aagtactcat ttagcgattt tgtcaaaata ggcgtg 286 18 262 DNA Homo sapien misc_feature (1)...(262) n = A,T,C or G 18tcggtcatag cagccccttc ttctcaattt catctgtcac taccctggtg tagtatctca 60tagccttaca tttttatagc ctcctccctg gtctgtcttt tgattttcct gcctgtaatc 120catatcacac ataactgcaa gtaaacattt ctaaagtgtg gttatgctca tgtcactcct 180gtgncaagaa atagtttcca ttaccgtctt aataaaattc ggatttgttc tttnctattn 240tcactcttca cctatgaccg aa 262 19 261 DNA Homo sapien 19tcggtcatag caaagccagt ggtttgagct ctctactgtg taaactccta aaccaaggcc 60atttatgata aatggtggca ggatttttat tataaacatg tacccatgca aatttcctat 120aactctgaga tatattcttc tacatttaaa caataaaaat aatctatttt taaaagccta 180atttgcgtag ttaggtaaga gtgtttaatg agagggtata aggtataaat caccagtcaa 240cgtttctctg cctatgaccg a 261 20 294 DNA Homo sapien misc_feature (1)...(294) n = A,T,C or G 20tacaacgagg cgacgtcggt aaaatcggac atgaagccac cgctggtctt ttcgtccgag 60cgataggcgc cggccagcca gcggaacggt tgcccggatg gcgaagcgag ccggagttct 120tcggactgag tatgaatctt gttgtgaaaa tactcgccgc cttcgttcga cgacgtcgcg 180tcgaaatctt cganctcctt acgatcgaag tcttcgtggg cgacgatcgc ggtcagttcc 240gccccaccga aatcatggtt gagccggatg ctgnccccga agncctcgtt tgtn 294 21 208 DNA Homo sapien misc_feature (1)...(208) n = A,T,C or G 21ttggtaaagg gcatggacgc agacgcctga cgtttggctg aaaatctttc attgattcgt 60atcaatgaat aggaaaattc ccaaagaggg aatgtcctgt tgctcgccag tttttntgtt 120gttctcatgg anaaggcaan gagctcttca gactattggn attntcgttc ggtcttctgc 180caactagtcg ncttgcnang atcttcat 208 22 287 DNA Homo sapien misc_feature (1)...(287) n = A,T,C or G 22nccnttgagc tgagtgattg agatntgtaa tggttgtaag ggtgattcag gcggattagg 60gtggcgggtc acccggcagt gggtctcccg acaggccagc aggatttggg gcaggtacgg 120ngtgcgcatc gctcgactat atgctatggc aggcgagccg tggaaggngg atcaggtcac 180ggcgctggag ctttccacgg tccatgnatt gngatggctg ttctaggcgg ctgttgccaa 240gcgtgatggt acgctggctg gagcattgat ttctggtgcc aaggtgg 287 23 204 DNA Homo sapien misc_feature (1)...(204) n = A,T,C or G 23ttgggtaaag ggagcaagga gaaggcatgg agaggctcan gctggtcctg gcctacgact 60gggccaagct gtcgccgggg atggtggaga actgaagcgg gacctcctcg aggtcctccg 120ncgttacttc nccgtccagg aggagggtct ttccgtggtc tnggaggagc ggggggagaa 180gatnctcctc atggtcnaca tccc 204 24 264 DNA Homo sapien misc_feature (1)...(264) n = A,T,C or G 24tggattggtc aggagcgggt agagtggcac cattgagggg atattcaaaa atattatttt 60gtcctaaatg atagttgctg agtttttctt tgacccatga gttatattgg agtttatttt 120ttaactttcc aatcgcatgg acatgttaga cttattttct gttaatgatt nctattttta 180ttaaattgga tttgagaaat tggttnttat tatatcaatt tttggtattt gttgagtttg 240acattatagc ttagtatgtg acca 264 25 376 DNA Homo sapien misc_feature (1)...(376) n = A,T,C or G 25ttacaacgag gggaaactcc gtctctacaa aaattaaaaa attagccagg tgtggtggtg 60tgcacccgca atcccagcta cttgggaggt tgagacacaa gantcaccta natgtgggag 120gtcaaggttg catgagtcat gattgtgcca ctgcactcca gcctgggtga cagaccgaga 180ccctgcctca anaganaang aataggaagt tcagaaatcn tggntgtggn gcccagcaat 240ctgcatctat ncaacccctg caggcaangc tgatgcagcc tangttcaag agctgctgtt 300tctggaggca gcagttnggg cttccatcca gtatcacggc cacactcgca cnagccatct 360gtcctccgtn tgtnac 376 26 372 DNA Homo sapien misc_feature (1)...(372) n = A,T,C or G 26ttacaacgag gggaaactcc gtctctacaa aaattaaaaa attagccagg tgtggtggtg 60tgcacctgta atcccagcta cttgggcggc tgagacacaa gaaccaccta aatgtgggag 120ggtcaaggtt gcatgagtca tgatcgcgcc actgcactcc agcctgggtg acagactgag 180accctgcctc aaaagaaaaa gaataggaag ttcagaaacc ctgggtgtgg ngcccagcaa 240tctgcattta aacaatccct gcaggcaatg ctgatgcagc ctaagttcaa gagctgctgt 300tctggaggca gnagtaaggg cttccatcca gcatcacggn caacactgca aaagcacctg 360tcctcgttgg ta 372 27 477 DNA Homo sapien 27ttctgtccac atctacaagt tttatttatt ttgtgggttt tcagggtgac taagtttttc 60cctacattga aaagagaagt tgctaaaagg tgcacaggaa atcatttttt taagtgaata 120tgataatatg ggtccgtgct taatacaact gagacatatt tgttctctgt ttttttagag 180tcacctctta aagtccaatc ccacaatggt gaaaaaaaaa tagaaagtat ttgttctacc 240tttaaggaga ctgcagggat tctccttgaa aacggagtat ggaatcaatc ttaaataaat 300atgaaattgg ttggtcttct gggataagaa attcccaact cagtgtgctg aaattcacct 360gacttttttt gggaaaaaat agtcgaaaat gtcaatttgg tccataaaat acatgttact 420attaaaagat atttaaagac aaattctttc agagctctaa gattggtgtg gacagaa 477 28 438 DNA Homo sapien misc_feature (1)...(438) n = A,T,C or G 28tctncaacct cttgantgtc aaaaaccttn taggctatct ctaaaagctg actggtattc 60attccagcaa aatccctcta gtttttggag tttcctttta ctatctgggg ctgcctgagc 120cacaaatgcc aaattaagag catggctatt ttcgggggct gacaggtcaa aaggggtgta 180aatccgataa gcctcctgga ggtgctctaa aaacactcct ggtgactcat catgcccctg 240gacgacttca atcgncttag acaagtttat aggtttctgg gcagctccct gaatacccac 300gaggagatac cggtggaaat cgtcaaaagt tctccctcca cttgagaaat ttgggtccca 360attaggtccc aattgggtct ctaatcacta ttcctctagc ttcctcctcc ggnctattgg 420ttgatgtgag gttgaaga 438 29 620 DNA Homo sapien misc_feature (1)...(620) n = A,T,C or G 29aagagggtac cagccccaag ccttgacaac ttccataggg tgtcaagcct gtgggtgcac 60agaagtcaaa aattgagttt tgggatcctc agcctagatt tcagaggata taaagaaaca 120cctaacacct agatattcag acaaaagttt actacaggga tgaagctttc acggaaaacc 180tctactagga aagtacagaa gagaaatgtg ggtttggagc ccccaaacag aatcccctct 240agaacactgc ctaatgaaac tgtgagaaga tggccactgt catccagaca ccagaatgat 300agacccacca aaaacttatg ccatattgcc tataaaacct acagacactc aatgccagcc 360ccatgaaaaa aaaactgaga agaagactgt nccctacaat gccaccggag cagaactgcc 420ccaggccatg gaagcacagc tcttatatca atgtgacctg gatgttgaga catggaatcc 480nangaaatcn ttttaanact tccacggttn aatgactgcc ctattanatt cngaacttan 540atccnggcct gtgacctctt tgctttggcc attccccctt tttggaatgg ctnttttttt 600cccatgcctg tnccctctta 620 30 100 DNA Homo sapien 30ttacaacgag ggggtcaatg tcataaatgt cacaataaaa caatctcttc tttttttttt 60tttttttttt tttttttttt tttttttttt tttttttttt 100 31 762 DNA Homo sapien misc_feature (1)...(762) n = A,T,C or G 31tagtctatgc gccggacaga gcagaattaa attggaagtt gccctccgga ctttctaccc 60acactcttcc tgaaaagaga aagaaaagag gcaggaaaga ggttaggatt tcattttcaa 120gagtcagcta attaggagag cagagtttag acagcagtag gcaccccatg atacaaacca 180tggacaaagt ccctgtttag taactgccag acatgatcct gctcaggttt tgaaatctct 240ctgcccataa aagatggaga gcaggagtgc catccacatc aacacgtgtc caagaaagag 300tctcagggag acaagggtat caaaaaacaa gattcttaat gggaaggaaa tcaaaccaaa 360aaattagatt tttctctaca tatatataat atacagatat ttaacacatt attccagagg 420tggctccagt ccttggggct tgagagatgg tgaaaacttt tgttccacat taacttctgc 480tctcaaattc tgaagtatat cagaatggga caggcaatgt tttgctccac actggggcac 540agacccaaat ggttctgtgc ccgaagaaga gaagcccgaa agacatgaag gatgcttaag 600gggggttggg aaagccaaat tggtantatc ttttcctcct gcctgtgttc cngaagtctc 660cnctgaagga attcttaaaa ccctttgtga ggaaatgccc ccttaccatg acaantggtc 720ccattgcttt tagggngatg gaaacaccaa gggttttgat cc 762 32 276 DNA Homo sapien 32tagtctatgc gtgtattaac ctcccctccc tcagtaacaa ccaaagaggc aggagctgtt 60attaccaacc ccattttaca gatgcatcaa taatgacaga gaagtgaagt gacttgcgca 120cacaaccagt aaattggcag agtcagattt gaatccatgg agtctggtct gcactttcaa 180tcaccgaata ccctttctaa gaaacgtgtg ctgaatgagt gcatggataa atcagtgtct 240actcaacatc tttgcctaga tatcccgcat agacta 276 33 477 DNA Homo sapien 33tagtagttgc caaatatttg aaaatttacc cagaagtgat tgaaaacttt ttggaaacaa 60aaacaaataa agccaaaagg taaaataaaa atatctttgc actctcgtta ttacctatcc 120ataacttttt caccgtaagc tctcctgctt gttagtgtag tgtggttata ttaaactttt 180tagttattat tttttattca cttttccact agaaagtcat tattgattta gcacacatgt 240tgatctcatt tcattttttc tttttatagg caaaatttga tgctatgcaa caaaaatact 300caagcccatt atcttttttc cccccgaaat ctgaaaattg caggggacag agggaagtta 360tcccattaaa aaattgtaaa tatgttcagt ttatgtttaa aaatgcacaa aacataagaa 420aattgtgttt acttgagctg ctgattgtaa gcagttttat ctcaggggca actacta 477 34 631 DNA Homo sapien 34tagtagttgc caattcagat gatcagaaat gctgctttcc tcagcattgt cttgttaaac 60cgcatgccat ttggaacttt ggcagtgaga agccaaaagg aagaggtgaa tgacatatat 120atatatatat attcaatgaa agtaaaatgt atatgctcat atactttcta gttatcagaa 180tgagttaagc tttatgccat tgggctgctg catattttaa tcagaagata aaagaaaatc 240tgggcatttt tagaatgtga tacatgtttt tttaaaactg ttaaatatta tttcgatatt 300tgtctaagaa ccggaatgtt cttaaaattt actaaaacag tattgtttga ggaagagaaa 360actgtactgt ttgccattat tacagtcgta caagtgcatg tcaagtcacc cactctctca 420ggcatcagta tccacctcat agctttacac attttgacgg ggaatattgc agcatcctca 480ggcctgacat ctgggaaagg ctcagatcca cctactgctc cttgctcgtt gatttgtttt 540aaaatattgt gcctggtgtc acttttaagc cacagccctg cctaaaagcc agcagagaac 600agaacccgca ccattctata ggcaactact a 631 35 578 DNA Homo sapien 35tagtagttgc catcccatat tacagaaggc tctgtataca tgacttattt ggaagtgatc 60tgttttctct ccaaacccat ttatcgtaat ttcaccagtc ttggatcaat cttggtttcc 120actgatacca tgaaacctac ttggagcaga cattgcacag ttttctgtgg taaaaactaa 180aggtttattt gctaagctgt catcttatgc ttagtatttt ttttttacag tggggaattg 240ctgagattac attttgttat tcattagata ctttgggata acttgacact gtcttctttt 300tttcgctttt aattgctatc atcatgcttt tgaaacaaga acacattagt cctcaagtat 360tacataagct tgcttgttac gcctggtggt ttaaaggact atctttggcc tcaggttcac 420aagaatgggc aaagtgtttc cttatgttct gtagttctca ataaaagatt gccaggggcc 480gggtactgtg gctcgcactg taatcccagc actttgggaa gctgaggctg gcggatcatg 540ttagggcagg tgttcgaaac cagcctgggc aactacta 578 36 583 DNA Homo sapien 36tagtagttgc ctgtaatccc agcaactcag gaggctgggg caggagaatc agttgaacct 60gggaggcaga agttgtaatt agcaaagatc gcaccattgc acttcagcct gggcaacaag 120agtgagattc catctcaaaa acaaaaaaaa gaaaaagaaa agaaaaggaa aaaacgtata 180aacccagcca aaacaaaatg atcattcttt taataagcaa gactaattta atgtgtttat 240ttaatcaaag cagttgaatc ttctgagtta ttggtgaaaa tacccatgta gttaatttag 300ggttcttact tgggtgaacg tttgatgttc acaggttata aaatggttaa caaggaaaat 360gatgcataaa gaatcttata aactactaaa aataaataaa atataaatgg ataggtgcta 420tggatggagt ttttgtgtaa tttaaaatct tgaagtcatt ttggatgctc attggttgtc 480tggtaatttc cattaggaaa aggttatgat atggggaaac tgtttctgga aattgcggaa 540tgtttctcat ctgtaaaatg ctagtatctc agggcaacta cta 583 37 716 DNA Homo sapien misc_feature (1)...(716) n = A,T,C or G 37gatctactag tcatntggat tctatccatg gcagctaagc ctttctgaat ggattctact 60gctttcttgt tctttaatcc agacccttat atatgtttat gttcacaggc agggcaatgt 120ttagtgaaaa caattctaaa ttttttattt tgcattttca tgctaatttc cgtcacactc 180cagcaggctt cctgggagaa taaggagaaa tacagctaaa gacattgtcc ctgcttactt 240acagcctaat ggtatgcaaa accacttcaa taaagtaaca ggaaaagtac taaccaggta 300gaatggacca aaactgatat agaaaaatca gaggaagaga ggaacaaata tttactgagt 360cctagaatgt acaaggcttt ttaattacat attttatgta aggcctgcaa aaaacaggtg 420agtaatcaac atttgtccca ttttacatat aaggaaactg aagcttaaat tgaataattt 480aatgcataga ttttatagtt agaccatgtt caggtcccta tgttatactt actagctgta 540tgaatatgag aaaataattt tgttattttc ttggcatcag tattttcatc tgcaaaataa 600agctaaagtt atttagcaaa cagtcagcat agtgcctgat acatagtagg tgctccaaac 660atgattacnc tantattngg tattanaaaa atccaatata ggcntggata aaaccg 716 38 688 DNA Homo sapien misc_feature (1)...(688) n = A,T,C or G 38ttctgtccac atatcatccc actttaattg ttaatcagca aaactttcaa tgaaaaatca 60tccattttaa ccaggatcac accaggaaac tgaaggtgta ttttttttta ccttaaaaaa 120aaaaaaaaaa accaaacaaa ccaaaacaga ttaacagcaa agagttctaa aaaatttaca 180tttctcttac aactgtcatt cagagaacaa tagttcttaa gtctgttaaa tcttggcatt 240aacagagaaa cttgatgaan agttgtactt ggaatattgt ggattttttt ttttgtctaa 300tctcccccta ttgttttgcc aacagtaatt taagtttgtg tggaacatcc ccgtagttga 360agtgtaaaca atgtatagga aggaatatat gataagatga tgcatcacat atgcattaca 420tgtagggacc ttcacaactt catgcactca gaaaacatgc ttgaagagga ggagaggacg 480gcccagggtc accatccagg tgccttgagg acagagaatg cagaagtggc actgttgaaa 540tttagaagac catgtgtgaa tggtttcagg cctgggatgt ttgccaccaa gaagtgcctc 600cgagaaattt ctttcccatt tggaatacag ggtggcttga tgggtacggt gggtgaccca 660acgaagaaaa tgaaattctg ccctttcc 688 39 585 DNA Homo sapien misc_feature (1)...(585) n = A,T,C or G 39tagtagttgc cgcnnaccta aaanttggaa agcatgatgt ctaggaaaca tantaaaata 60gggtatgcct atgtgctaca gagagatgtt agcatttaaa gtgcatantt ttatgtattt 120tgacaaatgc atatncctct ataatccaca actgattacg aagctattac aattaaaaag 180tttggccggg cgtggtgggc ggtggctgac gcctgtaatc ccagcacttt gggaggccga 240ggcacgcgga tcacgaggtc gggagttcaa gaccatcctg gctaacacgg tgaaagtcca 300tctctactaa aaatacgaaa aaattacccc ggcgtggtgg cgggcgcctg tagtcccagc 360tactccggag gctgaggcag gagaatggcg tgaacccagg acacggagct tgcagtgtgc 420caacatcacg tcactgccct ccagcctggg ggacaggaac aagantcccg tcctcanaaa 480agaaaaatac tactnatant ttcnacttta ttttaantta cacagaactn cctcttggta 540cccccttacc attcatctca cccacctcct atagggcacn nctaa 585 40 475 DNA Homo sapien 40tctgtccaca ccaatcttag aagctctgaa aagaatttgt ctttaaatat cttttaatag 60taacatgtat tttatggacc aaattgacat tttcgactgt tttttccaaa aaagtcaggt 120gaatttcagc acactgagtt gggaatttct tatcccagaa gaccaaccaa tttcatattt 180atttaagatt gattccatac tccgttttca aggagaatcc ctgcagtctc cttaaaggta 240gaacaaatac ttcctatttt tttttcacca ttgtgggatt ggactttaag aggtgactct 300aaaaaaacag agaacaaata tgtctcagtt gtattaagca cggacccata ttatcatatt 360cacttaaaaa aatgatttcc tgtgcacctt ttggcaactt ctcttttcaa tgtagggaaa 420aacttagtca ccctgaaaac ccacaaaata aataaaactt gtagatgtgg acaga 475 41 423 DNA Homo sapien 41taagagggta catcgggtaa gaacgtaggc acatctagag cttagagaag tctggggtag 60gaaaaaaatc taagtattta taagggtata ggtaacattt aaaagtaggg ctagctgaca 120ttatttagaa agaacacata cggagagata agggcaaagg actaagacca gaggaacact 180aatatttagt gatcacttcc attcttggta aaaatagtaa cttttaagtt agcttcaagg 240aagatttttg gccatgatta gttgtcaaaa gttagttctc ttgggtttat attactaatt 300ttgttttaag atccttgtta gtgctttaat aaagtcatgt tatatcaaac gctctaaaac 360attgtagcat gttaaatgtc acaatatact taccatttgt tgtatatggc tgtaccctct 420cta 423 42 527 DNA Homo sapien misc_feature (1)...(527) n = A,T,C or G 42tctcctaggc taatgtgtgt gtttctgtaa aagtaaaaag ttaaaaattt taaaaataga 60aaaaagctta tagaataaga atatgaagaa agaaaatatt tttgtacatt tgcacaatga 120gtttatgttt taagctaagt gttattacaa aagagccaaa aaggttttaa aaattaaaac 180gtttgtaaag ttacagtacc cttatgttaa tttataattg aagaaagaaa aacttttttt 240tataaatgta gtgtagccta agcatacagt atttataaag tctggcagtg ttcaataatg 300tcctaggcct tcacattcac tcactgactc acccagagca acttccagtc ctgtaagctc 360cattcgtggt aagtgcccta tacaggtgca ccatttattt tacagtattt ttactgtacc 420ttctctatgt ttccatatgt ttcgatatac aaataccact ggttactatn gcccnacagg 480taattccagt aacacggcct gtatacgtct ggtancccta gngaaga 527 43 331 DNA Homo sapien 43tcttcaacct cgtaggacaa ctctcatatg cctgggcact atttttaggt tactaccttg 60gctgcccttc tttaagaaaa aaaaaagaag aaaaaagaac ttttccacaa gtttctcttc 120ctctagttgg aaaattagag aaatcatgtt tttaattttg tgttatttca gatcacaaat 180tcaaacactt gtaaacatta agcttctgtt caatcccctg ggaagaggat tcattctgat 240atttacggtt caaaagaagt tgtaatattg tgcttggaac acagagaacc agttattaac 300ttcctactac tattatataa taaataataa c 331 44 592 DNA Homo sapien misc_feature (1)...(592) n = A,T,C or G 44ggcttagtag ttgccaggca aaatarcgtt gattctcctc aggagccacc cccaacaccc 60ctgtttgctt ctagacctat acctagacta aagtcccagc agacccctag aggtgaggtt 120cagagtgacc cttgaggaga tgtgctacac tagaaaagaa ctgcttgagt tttctaattt 180atataagcag aaatctggag aagagtcata ggaatggata ttaagggtgt gagataatgg 240cggaaggaat atagagttgg atcaggctgg acttattgat ttgaacccac taagtagaga 300ttctgctttt gatgttgcag ctcagggagt taaaaaaggt tttaatggtt ctaatagttt 360atttgcttgg ttagctgaaa tatggataaa agatggccca ctgtgagcaa gctggaaatg 420cctgatctct ctcagtttaa tgtagaggaa gggatccaaa agtttaggga ganttggatg 480ctggraktgg attggtcact ttgrgaccta cccwtcccag ctgggagggt ccagaagata 540cacccttgac caacgctttg cgaaatggat ttgtgatggc ggcaactact aa 592 45 567 DNA Homo sapien misc_feature (1)...(567) n = A,T,C or G 45ggcttagtag ttgccattgc gagtgcttgc tcaacgagcg ttgaacatgg cggattgtct 60agattcaacg gatttgagtt ttaccagcaa agcgaaccaa gcgcggccca gagaattatg 120ggttggttgg ctttgaaaag atggaaatcc tgtaggccta gtcagaaaag ccttcttgca 180gaacagttgg ttctcgggcg aacgctcatc aagatgccca ttggaaaggc tagcgtgtat 240ttgggagagc ctgatagcgt gtcttctgat gatgtttgtg cttggacagt gacaaaagat 300atgcaaagca agtccgaact agacgtcaag cttcgtgagc aaattattgt agactcctac 360ttatactgtg aggaatgata gccaagggtg gggactttaa gactaaggtg gtttgtactt 420gcgccgatga tcccaggcag aaagamctga tcgctagttt tatacgggca actactaagc 480cgaattccag cacactggcg gccgttacta attggatccg anctcggtac cagcttgatg 540catascttga gttwtctata ntgtcnc 567 46 908 DNA Homo sapien misc_feature (1)...(908) n = A,T,C or G 46gagcgaaaga ccgagggcag ngnntangng cgangaagcg gagagggcca aaaagcaacc 60gctttccccg gggggtgccg attcattaag gcaggtggag gacaggtttc ccgatggaag 120gcggcagggg cgcaagcaat taatgtgagt aggccattca ttagcacccg ggcttaacat 180ttaagcttcg ggttggtatg tggtgggaat tgtgagcgga taacaatttc acacaggaaa 240cagctatgac catgattacg ccaagctatt taggtgacat tatagaataa ctcaagttat 300gcatcaagct tggtaccgag ttcggatcca ctagtaacgg ccgccagtgt gtggaattcg 360gcttagtagt tgccgaccat ggagtgctac ctaggctaga atacctgagy tcctccctag 420cctcactcac attaaattgt atcttttcta cattagatgt cctcagcgcc ttatttctgc 480tggacwatcg ataaattaat cctgatagga tgatagcagc agattaatta ctgagagtat 540gttaatgtgt catccctcct atataacgta tttgcatttt aatggagcaa ttctggagat 600aatccctgaa ggcaaaggaa tgaatcttga gggtgagaaa gccagaatca gtgtccagct 660gcagttgtgg gagaaggtga tattatgtat gtctcagaag tgacaccata tgggcaacta 720ctaagcccga attccagcac actggcgggc gttactaatg gatccgagct cggtaccaag 780cttgatgcat agcttgagta tctatagtgt cactaaatag cctggcgtta tcatggtcat 840agctgtttcc tgtgtgaaat tgttatccgc tcccaattcc ccccaccata cgagccggaa 900cataaagt 908 47 480 DNA Homo sapien misc_feature (1)...(480) n = A,T,C or G 47tgccaacaag gaaagtttta aatttcccct tgaggattct tggtgatcat caaattcagt 60ggtttttaag gttgttttct gtcaaataac tctaacttta agccaaacag tatatggaag 120cacagataka atattacaca gataaaagag gagttgatct aaagtaraga tagttggggg 180ctttaatttc tggaacctag gtctccccat cttcttctgt gctgaggaac ttcttggaag 240cggggattct aaagttcttt ggaagacagt ttgaaaacca ccatgttgtt ctcagtacct 300ttatttttaa aaagtaggtg aacattttga gagagaaaag ggcttggttg agatgaagtc 360cccccccccc cttttttttt ttttagctga aatagatacc ctatgttnaa rgaarggatt 420attatttacc atgccaytar scacatgctc tttgatgggc nyctccstac cctccttaag 480 48 591 DNA Homo sapien 48aagagggtac cgagtggaat ttccgcttca ctagtctggt gtggctagtc ggtttcgtgg 60tggccaacat tacgaacttc caactcaacc gttcttggac gttcaagcgg gagtaccggc 120gaggatggtg gcgtgaattc tggcctttct ttgccgtggg atcggtagcc gccatcatcg 180gtatgtttat caagatcttc tttactaacc cgacctctcc gatttacctg cccgagccgt 240ggtttaacga ggggaggggg atccagtcac gcgagtactg gtcccagatc ttcgccatcg 300tcgtgacaat gcctatcaac ttcgtcgtca ataagttgtg gaccttccga acggtgaagc 360actccgaaaa cgtccggtgg ctgctgtgcg gtgactccca aaatcttgat aacaacaagg 420taaccgaatc gcgctaagga accccggcat ctcgggtact ctgcatatgc gtacccctta 480agccgaattc cagcacactg gcggccgtta ctaattggat ccgaactccg taaccaagcc 540tgatgcgtaa cttgagttat tctatagtgt ccctaaaata acctggcgtt a 591 49 454 DNA Homo sapien 49aagagggtac ctgccttgaa atttaaatgt ctaaggaaar tgggagatga ttaagagttg 60gtgtggcyta gtcacaccaa aatgtattta ttacatcctg ctcctttcta gttgacagga 120aagaaagctg ctgtggggaa aggagggata aatactgaag ggatttacta aacaaatgtc 180catcacagag ttttcctttt tttttttttg agacagagtc ttgctctgtc acccaggctg 240gaatgaagwg gtatgatctc agttgaatgc aacctctacc tcctaggttc aagcgattct 300catgcctcag cctcctgagc agctgggact ataggcgcat gctaccatgc caggctaatt 360tttatatttt tattagagac ggggtgttgc catgttggcc aggcaggtct cgaactcctg 420ggcctcagat gatctgcccc accgtaccct ctta 454 50 463 DNA Homo sapien 50aagagggtac caaaaaaaag aaaaaggaaa aaaagaaaaa caacttgtat aaggctttct 60gctgcataca gctttttttt tttaaataaa tggtgccaac aaatgttttt gcattcacac 120caattgctgg ttttgaaatc gtactcttca aaggtatttg tgcagatcaa tccaatagtg 180atgccccgta ggttttgtgg actgcccacg ttgtctacct tctcatgtag gagccattga 240gagactgttt ggacatgcct gtgttcatgt agccgtgatg tccgggggcc gtgtacatca 300tgttaccgtg gggtggggtc tgcattggct gctgggcata tggctgggtg cccatcatgc 360ccatctgcat ctgcataggg tattggggcg tttgatccat atagccatga ttgctgtggt 420agccactgtt catcattggc tgggacatgc tgttaccctc tta 463 51 399 DNA Homo sapien 51cttcaacctc ccaaagtgct gggattacag gactgagcca ccacgctcag cctaagcctc 60tttttcacta ccctctaagc gatctaccac agtgatgagg ggctaaagag cagtgcaatt 120tgattacaat aatggaactt agatttatta attaacaatt tttccttagc atgttggttc 180cataattatt aagagtatgg acttacttag aaatgagctt tcattttaag aatttcatct 240ttgaccttct ctattagtct gagcagtatg acactatacg tattttattt aactaaccta 300ccttgagcta ttacttttta aaaggctata tacatgaatg tgtattgtca actgtaaagc 360cccacagtat ttaattatat catgatgtct ttgaggttg 399 52 392 DNA Homo sapien 52cttcaacctc aatcaacctt ggtaattgat aaaatcatca cttaactttc tgatataatg 60gcaataatta tctgagaaaa aaaagtggtg aaagattaaa cttgcatttc tctcagaatc 120ttgaaggata tttgaataat tcaaaagcgg aatcagtagt atcagccgaa gaaactcact 180tagctagaac gttggaccca tggatctaag tccctgccct tccactaacc agctgattgg 240ttttgtgtaa acctcctaca cgcttgggct tggtcgcctc atttgtcaaa gtaaaggctg 300aaataggaag ataatgaacc gtgtcttttt ggtctctttt ccatccatta ctctgatttt 360acaaagaggc ctgtattccc ctggtgaggt tg 392 53 179 DNA Homo sapien misc_feature (1)...(179) n = A,T,C or G 53ttcgggtgat gcctcctcag gctacagtga agactggatt acagaaaggt gccagcgaga 60tttcagattc ctgtaaacct ctaaagaaaa ggagtcgcgc ctcaactgat gtagaaatga 120ctagttcagc atacngagac acntctgact ccgattctag aggactgagt gacctgcan 179 54 112 DNA Homo sapien misc_feature (1)...(112) n = A,T,C or G 54ttcgggtgat gcctcctcag gctacatcat natagaagca aagtagaana atcnngtttg 60tgcattttcc cacanacaaa attcaaatga ntggaagaaa ttggganagt at 112 55 225 DNA Homo sapien 55tgagcttccg cttctgacaa ctcaatagat aatcaaagga caactttaac agggattcac 60aaaggagtat atccaaatgc caataaacat ataaaaagga attcagcttc atcatcatca 120gaagwatgca aattaaaacc ataatgagaa accactatgt cccactagaa tagataaaat 180cttaaaagac tggtaaaacc aagtgttggt aaggcaagag gagca 225 56 175 DNA Homo sapien 56gctcctcttg ccttaccaac acattctcaa aaacctgtta gagtcctaag cattctcctg 60ttagtattgg gattttaccc ctgtcctata aagatgttat gtaccaaaaa tgaagtggag 120ggccataccc tgagggaggg gagggatctc tagtgttgtc agaagcggaa gctca 175 57 223 DNA Homo sapien 57agccatttac cacccatgga tgaatggatt ttgtaattct agctgttgta ttttgtgaat 60ttgttaattt tgttgttttt ctgtgaaaca catacattgg atatgggagg taaaggagtg 120tcccagttgc tcctggtcac tccctttata gccattactg tcttgtttct tgtaactcag 180gttaggtttt ggtctctctt gctccactgc aaaaaaaaaa aaa 223 58 211 DNA Homo sapien 58gttcgaaggt gaacgtgtag gtagcggatc tcacaactgg ggaactgtca aagacgaatt 60aactgacttg gatcaatcaa atgtgactga ggaaacacct gaaggtgaag aacatcatcc 120agtggcagac actgaaaata aggagaatga agttgaagag gtaaaagagg agggtccaaa 180agagatgact ttggatgggt ggtaaatggc t 211 59 208 DNA Homo sapien 59gctcctcttg ccttaccaac tttgcaccca tcatcaacca tgtggccagg tttgcagccc 60aggctgcaca tcaggggact gcctcgcaat acttcatgct gttgctgctg actgatggtg 120ctgtgacgga tgtggaagcc acacgtgagg ctgtggtgcg tgcctcgaac ctgcccatgt 180cagtgatcat tatgggtggt aaatggct 208 60 171 DNA Homo sapien 60agccatttac cacccatact aaattctagt tcaaactcca acttcttcca taaaacatct 60aaccactgac accagttggc aatagcttct tccttcttta acctcttaga gtatttatgg 120tcaatgccac acatttctgc aactgaataa agttggtaag gcaagaggag c 171 61 134 DNA Homo sapien misc_feature (1)...(134) n = A,T,C or G 61cgggtgatgc ctcctcaggc tttggtgtgt ccactcnact cactggcctc ttctccagca 60actggtgaan atgtcctcan gaaaancncc acacgcngct cagggtgggg tgggaancat 120canaatcatc nggc 134 62 145 DNA Homo sapien 62agagggtaca tatgcaacag tatataaagg aagaagtgca ctgagaggaa cttcatcaag 60gccatttaat caataagtga tagagtcaag gctcaaccca ggtgtgacgg attccaggtc 120ccaagctcct tactggtacc ctctt 145 63 297 DNA Homo sapien 63tgcactgaga ggaattcaaa gggtttatgc caaagaacaa accagtcctc tgcagcctaa 60ctcatttgtt tttgggctgc gaagccatgt agagggcgat caggcagtag atggtccctc 120ccacagtcag cgccatggtg gtccggtaaa gcatttggtc aggcaggcct cgtttcaggt 180agacgggcac acatcagctt tctggaaaaa cttttgtagc tctggagctt tgtttttccc 240agcataatca tacactgtgg aatcggaggt cagtttagtt ggtaaggcaa gaggagc 297 64 300 DNA Homo sapien 64gcactgagag gaacttccaa tactatgttg aataggagtg gtgagagagg gcatccttgt 60cttgtgccgg ttttcaaagg gaatgcttcc agcttttgcc cattcagtat aatattaaag 120aatgttttac cattttctgt cttgcctgtt tttctgtgtt tttgttggtc tcttcattct 180ccatttttag gcctttacat gttaggaata tatttctttt aatgatactt cacctttggt 240atcttttgtg agactctact catagtgtga taagcactgg gttggtaagg caagaggagc 300 65 203 DNA Homo sapien 65gctcctcttg ccttaccaac tcacccagta tgtcagcaat tttatcrgct ttacctacga 60aacagcctgt atccaaacac ttaacacact cacctgaaaa gttcaggcaa caatcgcctt 120ctcatgggtc tctctgctcc agttctgaac ctttctcttt tcctagaaca tgcatttarg 180tcgatagaag ttcctctcag tgc 203 66 344 DNA Homo sapien 66tacggggacc cctgcattga gaaagcgaga ctcactctga agctgaaatg ctgttgccct 60tgcagtgctg gtagcaggag ttctgtgctt tgtgggctaa ggctcctgga tgacccctga 120catggagaag gcagagttgt gtgccccttc tcatggcctc gtcaaggcat catggactgc 180cacacacaaa atgccgtttt tattaacgac atgaaattga aggagagaac acaattcact 240gatgtggctc gtaaccatgg atatggtcac atacagaggt gtgattatgt aaaggttaat 300tccacccacc tcatgtggaa actagcctca atgcaggggt ccca 344 67 157 DNA Homo sapien 67gcactgagag gaacttcgta gggaggttga actggctgct gaggaggggg aacaacaggg 60taaccagact gatagccatt ggatggataa tatggtggtt gaggagggac actacttata 120gcagagggtt gtgtatagcc tgaggaggca tcacccg 157 68 137 DNA Homo sapien 68gcactgagag gaacttctag aaagtgaaag tctagacata aaataaaata aaaatttaaa 60actcaggaga gacagcccag cacggtggct cacgcctgta atcccagaac tttgggagcc 120tgaggaggca tcacccg 137 69 137 DNA Homo sapien 69cgggtgatgc ctcctcaggc tgtattttga agactatcga ctggacttct tatcaactga 60agaatccgtt aaaaatacca gttgtattat ttctacctgt caaaatccat ttcaaatgtt 120gaagttcctc tcagtgc 137 70 220 DNA Homo sapien misc_feature (1)...(220) n = A,T,C or G 70agcatgttga gcccagacac gcaatctgaa tgagtgtgca cctcaagtaa atgtctacac 60gctgcctggt ctgacatggc acaccatcnc gtggagggca casctctgct cngcctacwa 120cgagggcant ctcatwgaca ggttccaccc accaaactgc aagaggctca nnaagtactr 180ccagggtmya sggacmasgg tgggaytyca ycacwcatct 220 71 353 DNA Homo sapien misc_feature (1)...(353) n = A,T,C or G 71cgttagggtc tctatccact gctaaaccat acacctgggt aaacagggac catttaacat 60tcccanctaa atatgccaag tgacttcaca tgtttatctt aaagatgtcc aaaacgcaac 120tgattttctc ccctaaacct gtgatggtgg gatgattaan cctgagtggt ctacagcaag 180ttaagtgcaa ggtgctaaat gaangtgacc tgagatacag catctacaag gcagtacctc 240tcaacncagg gcaactttgc ttctcanagg gcatttagca gtgtctgaag taatttctgt 300attacaactc acggggcggg gggtgaatat ctantggana gnagacccta acg 353 72 343 DNA Homo sapien 72gcactgagag gaacttccaa tacyatkatc agagtgaaca rgcarccyac agaacaggag 60aaaatgttyg caatctctcc atctgacaaa aggctaatat ccagawtcta awaggaactt 120aaacaaattt atgagaaaag aacaracaac ctcawcaaaa agtgggtgaa ggawatgcts 180aaargaagac atytattcag ccagtaaaca yatgaaaaaa aggctcatsa tcactgawca 240ttagagaaat gcaaatcaaa accacaatga gataccatct yayrccagtt agaayggtga 300tcattaaaar stcaggaaac aacagatgct ggacaaggtg tca 343 73 321 DNA Homo sapien misc_feature (1)...(321) n = A,T,C or G 73gcactgagag gaacttcaga gagagagaga gagttccacc ctgtacttgg ggagagaaac 60agaaggtgag aaagtctttg gttctgaagc agcttctaag atcttttcat ttgcttcatt 120tcaaagttcc catgctgcca aagtgccatc ctttggggta ctgttttctg agctccagtg 180ataactcatt tatacaaggg agatacccag aaaaaaagtg agcaaatctt aaaaaggtgg 240cttgagttca gccttaaata ccatcttgaa atgacacaga gaaagaanga tgttgggtgg 300gagtggatag agaccctaac g 321 74 321 DNA Homo sapien 74gcactgagag gaacttcaga gagagagaga gagttccacc ctgtacttgg ggagagaaac 60agaaggtgag aaagtctttg gttctgaagc agcttctaag atcttttcat ttgcttcatt 120tcaaagttcc catgctgcca aagtgccatc ctttggggta ctgttttctg agctccagtg 180ataactcatt tatacaaggg agatacccag aaaaaaagtg agcaaatctt aaaaaggtgg 240cttgagttca gycttaaata ccatcttgaa atgamacaga gaaagaagga tgttgggtgg 300gagtggatag agaccctaac g 321 75 317 DNA Homo sapien 75gcactgagag gaacttccac atgcactgag aaatgcatgt tcacaaggac tgaagtctgg 60aactcagttt ctcagttcca atcctgattc aggtgtttac cagctacaca accttaagca 120agtcagataa ccttagcttc ctcatatgca aaatgagaat gaaaagtact catcgctgaa 180ttgttttgag gattagaaaa acatctggca tgcagtagaa attcaattag tattcatttt 240cattcttcta aattaaacaa ataggatttt tagtggtgga acttcagaca ccagaaatgg 300gagtggatag agaccct 317 76 244 DNA Homo sapien 76cgttagggtc tctatccact cccactactg atcaaactct atttatttaa ttatttttat 60catactttaa gttctgggat acacgtgcag catgcgcagg tttgttgcat aggtatacac 120ttgccatggt ggtttgctgc acccatcagt ccatcatcta cattaggtat ttctcctaat 180gctatccctc ccctagcccc ttacaccccc aacaggctct agtgtgtgaa gttcctctca 240gtgc 244 77 254 DNA Homo sapien 77cgttagggtc tctatccact gaaatctgaa gcacaggagg aagagaagca gtyctagtga 60gatggcaagt tcwtttacca cactctttaa catttygttt agttttaacc tttatttatg 120gataataaag gttaatatta ataatgattt attttaaggc attcccraat ttgcataatt 180ctccttttgg agataccctt ttatctccag tgcaagtctg gatcaaagtg atasamagaa 240gttcctctca gtgc 254 78 355 DNA Homo sapien misc_feature (1)...(355) n = A,T,C or G 78ttcgatacag gcaaacatga actgcaggag ggtggtgacg atcatgatgt tgccgatggt 60ccggatggnc acgaagacgc actggancac gtgcttacgt ccttttgctc tgttgatggc 120cctgagggga cgcaggaccc ttatgaccct cagaatcttc acaacgggag atggcactgg 180attgantccc antgacacca gagacacccc aaccaccagn atatcantat attgatgtag 240ttcctgtaga nggccccctt gtggaggaaa gctccatnag ttggtcatct tcaacaggat 300ctcaacagtt tccgatggct gtgatgggca tagtcatant taaccntgtn tcgaa 355 79 406 DNA Homo sapien 79taagagggta ccagcagaaa ggttagtatc atcagatagc atcttatacg agtaatatgc 60ctgctatttg aagtgtaatt gagaaggaaa attttagcgt gctcactgac ctgcctgtag 120ccccagtgac agctaggatg tgcattctcc agccatcaag agactgagtc aagttgttcc 180ttaagtcaga acagcagact cagctctgac attctgattc gaatgacact gttcaggaat 240cggaatcctg tcgattagac tggacagctt gtggcaagtg aatttgcctg taacaagcca 300gattttttaa aatttatatt gtaaataatg tgtgtgtgtg tgtgtgtata tatatatata 360tgtacagtta tctaagttaa tttaaaagtt gtttggtacc ctctta 406 80 327 DNA Homo sapien 80tttttttttt tttactcggc tcagtctaat cctttttgta gtcactcata ggccagactt 60agggctagga tgatgattaa taagagggat gacataacta ttagtggcag gttagttgtt 120tgtagggctc atggtagggg taaaaggagg gcaatttcta gatcaaataa taagaaggta 180atagctacta agaagaattt tatggagaaa gggacgcggg cgggggatat agggtcgaag 240ccgcactcgt aaggggtgga tttttctatg tagccgttga gttgtggtag tcaaaatgta 300ataattatta gtagtaagcc taggaga 327 81 318 DNA Homo sapien 81tagtctatgc ggttgattcg gcaatccatt atttgctgga ttttgtcatg tgttttgcca 60attgcattca taatttatta tgcatttatg cttgtatctc ctaagtcatg gtatataatc 120catgcttttt atgttttgtc tgacataaac tcttatcaga gccctttgca cacagggatt 180caataaatat taacacagtc tacatttatt tggtgaatat tgcatatctg ctgtactgaa 240agcacattaa gtaacaaagg caagtgagaa gaatgaaaag cactactcac aacagttatc 300atgattgcgc atagacta 318 82 338 DNA Homo sapien 82tcttcaacct ctactcccac taatagcttt ttgatgactt ctagcaagcc tcgctaacct 60cgccttaccc cccactatta acctactggg agaactctct gtgctagtaa ccacgttctc 120ctgatcaaat atcactctcc tacttacagg actcaacata ctagtcacag ccctatactc 180cctctacata tttaccacaa cacaatgggg ctcactcacc caccacatta acaacataaa 240accctcattc acacgagaaa acaccctcat gttcatacac ctatccccca ttctcctcct 300atccctcaac cccgacatca ttaccgggtt ttcctctt 338 83 111 DNA Homo sapien 83agccatttac cacccatcca caaaaaaaaa aaaaaaaaag aaaaatatca aggaataaaa 60atagactttg aacaaaaagg aacatttgct ggcctgagga ggcatcaccc g 111 84 224 DNA Homo sapien 84tcgggtgatg cctcctcagg ccaagaagat aaagcttcag acccctaaca catttccaaa 60aaggaagaaa ggagaaaaaa gggcatcatc cccgttccga agggtcaggg aggaggaaat 120tgaggtggat tcacgagttg cggacaactc ctttgatgcc aagcgaggtg cagccggaga 180ctggggagag cgagccaatc aggttttgaa gttcctctca gtgc 224 85 348 DNA Homo sapien 85gcactgagag gaacttcgtt ggaaacgggt ttttttcatg taaggctaga cagaagaatt 60ctcagtaact tccttgtgtt gtgtgtattc aactcacasa gttgaacgat cctttacaca 120gagcagactt gtaacactct twttgtggaa tttgcaagtg gagatttcag scgctttgaa 180gtsaaaggta gaaaaggaaa tatcttccta taaaaactag acagaatgat tctcagaaac 240tcctttgtga tgtgtgcgtt caactcacag agtttaacct ttcwtttcat agaagcagtt 300aggaaacact ctgtttgtaa agtctgcaag tggatagaga ccctaacg 348 86 293 DNA Homo sapien 86gcactgagag gaacttcytt gtgwtgtktg yattcaactc acagagttga asswtsmttt 60acabagwkca ggcttkcaaa cactcttttt gtmgaatytg caagwggaka tttsrrccrc 120tttgwggycw wysktmgaaw mggrwatatc ttcwyatmra amctagacag aaksattctc 180akaawstyyy ytgtgawgws tgcrttcaac tcacagagkt kaacmwtyct kytsatrgag 240cagttwkgaa actctmtttc tttggattct gcaagtggat agagacccta acg 293 87 10 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 87ctcctaggct 10 88 10 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 88agtagttgcc 10 89 11 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 89ttccgttatg c 11 90 10 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 90tggtaaaggg 10 91 10 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 91tcggtcatag 10 92 10 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 92tacaacgagg 10 93 10 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 93tggattggtc 10 94 10 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 94ctttctaccc 10 95 10 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 95ttttggctcc 10 96 10 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 96ggaaccaatc 10 97 10 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 97tcgatacagg 10 98 10 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 98ggtactaagg 10 99 10 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 99agtctatgcg 10 100 10 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 100ctatccatgg 10 101 10 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 101tctgtccaca 10 102 10 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 102aagagggtac 10 103 10 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 103cttcaacctc 10 104 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 104gctcctcttg ccttaccaac 20 105 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 105gtaagtcgag cagtgtgatg 20 106 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 106gtaagtcgag cagtctgatg 20 107 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 107gacttagtgg aaagaatgta 20 108 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 108gtaattccgc caaccgtagt 20 109 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 109atggttgatc gatagtggaa 20 110 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 110acggggaccc ctgcattgag 20 111 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 111tattctagac cattcgctac 20 112 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 112acataaccac tttagcgttc 20 113 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 113cgggtgatgc ctcctcaggc 20 114 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 114agcatgttga gcccagacac 20 115 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 115gacaccttgt ccagcatctg 20 116 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 116tacgctgcaa cactgtggag 20 117 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 117cgttagggtc tctatccact 20 118 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 118agactgactc atgtccccta 20 119 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 119tcatcgctcg gtgactcaag 20 120 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 120caagattcca taggctgacc 20 121 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 121acgtactggt cttgaaggtc 20 122 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 122gacgcttggc cacttgacac 20 123 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 123gtatcgacgt agtggtctcc 20 124 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 124tagtgacatt acgacgctgg 20 125 20 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 125cgggtgatgc ctcctcaggc 20 126 23 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 126atggctattt tcgggggctg aca 23 127 22 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 127ccggtatctc ctcgtgggta tt 22 128 18 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 128ctgcctgagc cacaaatg 18 129 24 DNA Artificial Sequence Primer for amplification from breast tumor cDNA 129ccggaggagg aagctagagg aata 24 130 14 DNA Artificial Sequence Primer 130tttttttttt ttag 14 131 18 PRT Artificial Sequence Predicited Th Motifs (B-cell epitopes) 131Ser Ser Gly Gly Arg Thr Phe Asp Asp Phe His Arg Tyr Leu Leu Val 1 5 10 15Gly Ile 132 22 PRT Artificial Sequence Predicited Th Motifs (B-cell epitopes) 132Gln Gly Ala Ala Gln Lys Pro Ile Asn Leu Ser Lys Xaa Ile Glu Val 1 5 10 15Val Gln Gly His Asp Glu 20 133 23 PRT Artificial Sequence Predicited Th Motifs (B-cell epitopes) 133Ser Pro Gly Val Phe Leu Glu His Leu Gln Glu Ala Tyr Arg Ile Tyr 1 5 10 15Thr Pro Phe Asp Leu Ser Ala 20 134 9 PRT Artificial Sequence Predicited HLA A2.1 Motifs (T-cell epitopes) 134Tyr Leu Leu Val Gly Ile Gln Gly Ala 1 5 135 9 PRT Artificial Sequence Predicited HLA A2.1 Motifs (T-cell epitopes) 135Gly Ala Ala Gln Lys Pro Ile Asn Leu 1 5 136 9 PRT Artificial Sequence Predicited HLA A2.1 Motifs (T-cell epitopes) 136Asn Leu Ser Lys Xaa Ile Glu Val Val 1 5 137 9 PRT Artificial Sequence Predicited HLA A2.1 Motifs (T-cell epitopes) 137Glu Val Val Gln Gly His Asp Glu Ser 1 5 138 9 PRT Artificial Sequence Predicited HLA A2.1 Motifs (T-cell epitopes) 138His Leu Gln Glu Ala Tyr Arg Ile Tyr 1 5 139 9 PRT Artificial Sequence Predicited HLA A2.1 Motifs (T-cell epitopes) 139Asn Leu Ala Phe Val Ala Gln Ala Ala 1 5 140 9 PRT Artificial Sequence Predicited HLA A2.1 Motifs (T-cell epitopes) 140Phe Val Ala Gln Ala Ala Pro Asp Ser 1 5 141 9388 DNA Homo sapien 141gctcgcggcc gcgagctcaa ttaaccctca ctaaagggag tcgactcgat cagactgtta 60ctgtgtctat gtagaaagaa gtagacataa gagattccat tttgttctgt actaagaaaa 120attcttctgc cttgagatgc tgttaatctg taaccctagc cccaaccctg tgctcacaga 180gacatgtgct gtgttgactc aaggttcaat ggatttaggg ctatgctttg ttaaaaaagt 240gcttgaagat aatatgcttg ttaaaagtca tcaccattct ctaatctcaa gtacccaggg 300acacaataca ctgcggaagg ccgcagggac ctctgtctag gaaagccagg tattgtccaa 360gatttctccc catgtgatag cctgagatat ggcctcatgg gaagggtaag acctgactgt 420cccccagccc gacatccccc agcccgacat cccccagccc gacacccgaa aagggtctgt 480gctgaggagg attagtaaaa gaggaaggcc tctttgcagt tgaggtaaga ggaaggcatc 540tgtctcctgc tcgtccctgg gcaatagaat gtcttggtgt aaaacccgat tgtatgttct 600acttactgag ataggagaaa acatccttag ggctggaggt gagacacgct ggcggcaata 660ctgctcttta atgcaccgag atgtttgtat aagtgcacat caaggcacag cacctttcct 720taaacttatt tatgacacag agacctttgt tcacgttttc ctgctgaccc tctccccact 780attaccctat tggcctgcca catccccctc tccgagatgg tagagataat gatcaataaa 840tactgaggga actcagagac cagtgtccct gtaggtcctc cgtgtgctga gcgccggtcc 900cttgggctca cttttctttc tctatacttt gtctctgtgt ctctttcttt tctcagtctc 960tcgttccacc tgacgagaaa tacccacagg tgtggagggg caggccaccc cttcaataat 1020ttactagcct gttcgctgac aacaagactg gtggtgcaga aggttgggtc ttggtgttca 1080ccgggtggca ggcatgggcc aggtgggagg gtctccagcg cctggtgcaa atctccaaga 1140aagtgcagga aacagcacca agggtgattg taaattttga tttggcgcgg caggtagcca 1200ttccagcgca aaaatgcgca ggaaagcttt tgctgtgctt gtaggcaggt aggccccaag 1260cacttcttat tggctaatgt ggagggaacc tgcacatcca ttggctgaaa tctccgtcta 1320tttgaggctg actgagcgcg ttcctttctt ctgtgttgcc tggaaacgga ctgtctgcct 1380agtaacatct gatcacgttt cccattggcc gccgtttccg gaagcccgcc ctcccatttc 1440cggaagcctg gcgcaaggtt ggtctgcagg tggcctccag gtgcaaagtg ggaagtgtga 1500gtcctcagtc ttgggctatt cggccacgtg cctgccggac atgggacgct ggagggtcag 1560cagcgtggag tcctggcctt ttgcgtccac gggtgggaaa ttggccattg ccacggcggg 1620aactgggact caggctgccc cccggccgtt tctcatccgt ccaccggact cgtgggcgct 1680cgcactggcg ctgatgtagt ttcctgacct ctgacccgta ttgtctccag attaaaggta 1740aaaacggggc tttttcagcc cactcgggta aaacgccttt tgatttctag gcaggtgttt 1800tgttgcacgc ctgggaggga gtgacccgca ggttgaggtt tattaaaata cattcctggt 1860ttatgttatg tttataataa agcaccccaa cctttacaaa atctcacttt ttgccagttg 1920tattatttag tggactgtct ctgataagga cagccagtta aaatggaatt ttgttgttgc 1980taattaaacc aatttttagt tttggtgttt gtcctaatag caacaacttc tcaggcttta 2040taaaaccata tttcttgggg gaaatttctg tgtaaggcac agcgagttag tttggaattg 2100ttttaaagga agtaagttcc tggttttgat atcttagtag tgtaatgccc aacctggttt 2160ttactaaccc tgtttttaga ctctcccttt ccttaaatca cctagccttg tttccacctg 2220aattgactct cccttagcta agagcgccag atggactcca tcttggctct ttcactggca 2280gccccttcct caaggactta acttgtgcaa gctgactccc agcacatcca agaatgcaat 2340taactgttaa gatactgtgg caagctatat ccgcagttcc gaggaattca tccgattgat 2400tatgcccaaa agccccgcgt ctatcacctt gtaataatct taaagcccct gcacctggaa 2460ctattaactt tcctgtaacc atttatcctt ttaacttttt tgcttacttt atttctgtaa 2520aattgtttta actagacctc ccctcccctt tctaaaccaa agtataaaag aagatctagc 2580cccttcttca gagcggagag aattttgagc attagccatc tcttggcggc cagctaaata 2640aatggacttt taatttgtct caaagtgtgg cgttttctct aactcgctca ggtacgacat 2700ttggaggccc cagcgagaaa cgtcaccggg agaaacgtca ccgggcgaga gccgggcccg 2760ctgtgtgctc ccccggaagg acagccagct tgtagggggg agtgccacct gaaaaaaaaa 2820tttccaggtc cccaaagggt gaccgtcttc cggaggacag cggatcgact accatgcggg 2880tgcccaccaa aattccacct ctgagtcctc aactgctgac cccggggtca ggtaggtcag 2940atttgacttt ggttctggca gagggaagcg accctgatga gggtgtccct cttttgactc 3000tgcccatttc tctaggatgc tagagggtag agccctggtt ttctgttaga cgcctctgtg 3060tctctgtctg ggagggaagt ggccctgaca ggggccatcc cttgagtcag tccacatccc 3120aggatgctgg gggactgagt cctggtttct ggcagactgg tctctctctc tctctttttc 3180tatctctaat ctttccttgt tcaggtttct tggagaatct ctgggaaaga aaaaagaaaa 3240actgttataa actctgtgtg aatggtgaat gaatggggga ggacaagggc ttgcgcttgt 3300cctccagttt gtagctccac ggcgaaagct acggagttca agtgggccct cacctgcggt 3360tccgtggcga cctcataagg cttaaggcag catccggcat agctcgatcc gagccggggg 3420tttataccgg cctgtcaatg ctaagaggag cccaagtccc ctaaggggga gcggccaggc 3480gggcatctga ctgatcccat cacgggaccc cctccccttg tttgtctaaa aaaaaaaaaa 3540gaagaaactg tcataactgt ttacatgccc tagggtcaac tgtttgtttt atgtttattg 3600ttctgttcgg tgtctattgt cttgtttagt ggttgtcaag gttttgcatg tcaggacgtc 3660gatattgccc aagacgtctg ggtaagaact tctgcaaggt ccttagtgct gattttttgt 3720cacaggaggt taaatttctc atcaatcatt taggctggcc accacagtcc tgtcttttct 3780gccagaagca agtcaggtgt tgttacggga atgagtgtaa aaaaacattc gcctgattgg 3840gatttctggc accatgatgg ttgtatttag attgtcatac cccacatcca ggttgattgg 3900acctcctcta aactaaactg gtggtgggtt caaaacagcc accctgcaga tttccttgct 3960cacctctttg gtcattctgt aacttttcct gtgcccttaa atagcacact gtgtagggaa 4020acctaccctc gtactgcttt acttcgttta gattcttact ctgttcctct gtggctactc 4080tcccatctta aaaacgatcc aagtggtcct tttcctcctc cctgccccct accccacaca 4140tctcgttttc cagtgcgaca gcaagttcag cgtctccagg acttggctct gctctcactc 4200cttgaaccct taaaagaaaa agctgggttt gagctatttg cctttgagtc atggagacac 4260aaaaggtatt tagggtacag atctagaaga agagagagaa cacctagatc caactgaccc 4320aggagatctc gggctggcct ctagtcctcc tccctcaatc ttaaagctac agtgatgtgg 4380caagtggtat ttagctgttg tggtttttct gctctttctg gtcatgttga ttctgttctt 4440tcgatactcc agccccccag ggagtgagtt tctctgtctg tgctgggttt gatatctatg 4500ttcaaatctt attaaattgc cttcaaaaaa aaaaaaaaaa gggaaacact tcctcccagc 4560cttgtaaggg ttggagccct ctccagtata tgctgcagaa tttttctctc ggtttctcag 4620aggattatgg agtccgcctt aaaaaaggca agctctggac actctgcaaa gtagaatggc 4680caaagtttgg agttgagtgg ccccttgaag ggtcactgaa cctcacaatt gttcaagctg 4740tgtggcgggt tgttactgaa actcccggcc tccctgatca gtttccctac attgatcaat 4800ggctgagttt ggtcaggagc accccttcca tggctccact catgcaccat tcataatttt 4860acctccaagg tcctcctgag ccagaccgtg ttttcgcctc gaccctcagc cggttcagct 4920cgccctgtac tgcctctctc tgaagaagag gagagtctcc ctcacccagt cccaccgcct 4980taaaaccagc ctactccctt agggtcatcc catgtctcct cggctatgtc ccctgtaggc 5040tcatcaccca ttgcctcttg gttgcaaccg tggtgggagg aagtagcccc tctactacca 5100ctgagagagg cacaagtccc tctgggtgat gagtgctcca cccccttcct ggtttatgtc 5160ccttctttct acttctgact tgtataattg gaaaacccat aatcctccct tctctgaaaa 5220gccccaggct ttgacctcac tgatggagtc tgtactctgg acacattggc ccacctggga 5280tgactgtcaa cagctccttt tgaccctttt cacctctgaa gagagggaaa gtatccaaag 5340agaggccaaa aagtacaacc tcacatcaac caataggccg gaggaggaag ctagaggaat 5400agtgattaga gacccaattg ggacctaatt gggacccaaa tttctcaagt ggagggagaa 5460cttttgacga tttccaccgg tatctcctcg tgggtattca gggagctgct cagaaaccta 5520taaacttgtc taaggcgact gaagtcgtcc aggggcatga tgagtcacca ggagtgtttt 5580tagagcacct ccaggaggct tatcggattt acaccccttt tgacctggca gcccccgaaa 5640atagccatgc tcttaatttg gcatttgtgg ctcaggcagc cccagatagt aaaaggaaac 5700tccaaaaact agagggattt tgctggaatg aataccagtc agcttttaga gatagcctaa 5760aaggtttttg acagtcaaga ggttgaaaaa caaaaacaag cagctcaggc agctgaaaaa 5820agccactgat aaagcatcct ggagtatcag agtttactgt tagatcagcc tcatttgact 5880tcccctccca catggtgttt aaatccagct acactacttc ctgactcaaa ctccactatt 5940cctgttcatg actgtcagga actgttggaa actactgaaa ctggccgacc tgatcttcaa 6000aatgtgcccc taggaaaggt ggatgccacc gtgttcacag acagtagcag cttcctcgag 6060aagggactac gaaaggccgg tgcagctgtt accatggaga cagatgtgtt gtgggctcag 6120gctttaccag caaacacctc agcacaaaag gctgaattga tcgccctcac tcaggctctc 6180cgatggggta aggatattaa cgttaacact gacagcaggt acgcctttgc tactgtgcat 6240gtacgtggag ccatctacca ggagcgtggg ctactcacct cagcaggtgg ctgtaatcca 6300ctgtaaagga catcaaaagg aaaacacggc tgttgcccgt ggtaaccaga aagctgattc 6360agcagctcaa gatgcagtgt gactttcagt cacgcctcta aacttgctgc ccacagtctc 6420ctttccacag ccagatctgc ctgacaatcc cgcatactca acagaagaag aaaactggcc 6480tcagaactca gagccaataa aaatcaggaa ggttggtgga ttcttcctga ctctagaatc 6540ttcatacccc gaactcttgg gaaaacttta atcagtcacc tacagtctac cacccattta 6600ggaggagcaa agctacctca gctcctccgg agccgtttta agatccccca tcttcaaagc 6660ctaacagatc aagcagctct ccggtgcaca acctgcgccc aggtaaatgc caaaaaaggt 6720cctaaaccca gcccaggcca ccgtctccaa gaaaactcac caggagaaaa gtgggaaatt 6780gactttacag aagtaaaacc acaccgggct gggtacaaat accttctagt actggtagac 6840accttctctg gatggactga agcatttgct accaaaaacg aaactgtcaa tatggtagtt 6900aagtttttac tcaatgaaat catccctcga cgtgggctgc ctgttgccat agggtctgat 6960aatggaccgg ccttcgcctt gtctatagtt tagtcagtca gtaaggcgtt aaacattcaa 7020tggaagctcc attgtgccta tcgaccccag agctctgggc aagtagaacg catgaactgc 7080accctaaaaa acactcttac aaaattaatc ttagaaaccg gtgtaaattg tgtaagtctc 7140cttcctttag ccctacttag agtaaggtgc accccttact gggctgggtt cttacctttt 7200gaaatcatgt atgggagggc gctgcctatc ttgcctaagc taagagatgc ccaattggca 7260aaaatatcac aaactaattt attacagtac ctacagtctc cccaacaggt acaagatatc 7320atcctgccac ttgttcgagg aacccatccc aatccaattc ctgaacagac agggccctgc 7380cattcattcc cgccaggtga cctgttgttt gttaaaaagt tccagagaga aggactccct 7440cctgcttgga agagacctca caccgtcatc acgatgccaa cggctctgaa ggtggatggc 7500attcctgcgt ggattcatca ctcccgcatc aaaaaggcca acggagccca actagaaaca 7560tgggtcccca gggctgggtc aggcccctta aaactgcacc taagttgggt gaagccatta 7620gattaattct ttttcttaat tttgtaaaac aatgcatagc ttctgtcaaa cttatgtatc 7680ttaagactca atataacccc cttgttataa ctgaggaatc aatgatttga ttccccaaaa 7740acacaagtgg ggaatgtagt gtccaacctg gtttttacta accctgtttt tagactctcc 7800ctttccttta atcactcagc cttgtttcca cctgaattga ctctccctta gctaagagcg 7860ccagatggac tccatcttgg ctctttcact ggcagccgct tcctcaagga cttaacttgt 7920gcaagctgac tcccagcaca tccaagaatg caattaactg ataagatact gtggcaagct 7980atatccgcag ttcccaggaa ttcgtccaat tgattacacc caaaagcccc gcgtctatca 8040ccttgtaata atcttaaagc ccctgcacct ggaactatta acgttcctgt aaccatttat 8100ccttttaact tttttgccta ctttatttct gtaaaattgt tttaactaga ccccccctct 8160cctttctaaa ccaaagtata aaagcaaatc tagccccttc ttcaggccga gagaatttcg 8220agcgttagcc gtctcttggc caccagctaa ataaacggat tcttcatgtg tctcaaagtg 8280tggcgttttc tctaactcgc tcaggtacga ccgtggtagt attttcccca acgtcttatt 8340tttagggcac gtatgtagag taacttttat gaaagaaacc agttaaggag gttttgggat 8400ttcctttatc aactgtaata ctggttttga ttatttattt atttatttat tttttttgag 8460aaggagtttc actcttgttg cccaggctgg agtgcaatgg tgcgatcttg gctcactgca 8520acttccgcct cccaggttca agcgattctc ctgcctcagc ctcgagagta gctgggatta 8580taggcatgcg ccaccacacc cagctaattt tgtattttta gtaaagatgg ggtttcttca 8640tgttggtcaa gctggtctgg aactccccgc ctcgggtgat ctgcccgcct cggcctccga 8700aagtgctggg attacaggtg tgatccacca cacccagccg atttatatgt atataaatca 8760cattcctcta accaaaatgt agtgtttcct tccatcttga atataggctg tagaccccgt 8820gggtatggga cattgttaac agtgagacca cagcagtttt tatgtcatct gacagcatct 8880ccaaatagcc ttcatggttg tcactgcttc ccaagacaat tccaaataac acttcccagt 8940gatgacttgc tacttgctat tgttacttaa tgtgttaagg tggctgttac agacactatt 9000agtatgtcag gaattacacc aaaatttagt ggctcaaaca atcattttat tatgtatgtg 9060gattctcatg gtcaggtcag gatttcagac agggcacaag ggtagcccac ttgtctctgt 9120ctatgatgtc tggcctcagc acaggagact caacagctgg ggtctgggac catttggagg 9180cttgttccct cacatctgat acctggcttg ggatgttgga agagggggtg agctgagact 9240gagtgcctat atgtagtgtt tccatatggc cttgacttcc ttacagcctg gcagcctcag 9300ggtagtcaga attcttagga ggcacagggc tccagggcag atgctgaggg gtcttttatg 9360aggtagcaca gcaaatccac ccaggatc 9388 142 419 DNA Homo sapien 142tgtaagtcga gcagtgtgat ggaaggaatg gtctttggag agagcatatc catctcctcc 60tcactgcctc ctaatgtcat gaggtacact gagcagaatt aaacagggta gtcttaacca 120cactattttt agctaccttg tcaagctaat ggttaaagaa cacttttggt ttacacttgt 180tgggtcatag aagttgcttt ccgccatcac gcaataagtt tgtgtgtaat cagaaggagt 240taccttatgg tttcagtgtc attctttagt taacttggga gctgtgtaat ttaggctttg 300cgtattattt cacttctgtt ctccacttat gaagtgattg tgtgttcgcg tgtgtgtgcg 360tgcgcatgtg cttccggcag ttaacataag caaataccca acatcacact gctcgactt 419 143 402 DNA Homo sapien 143tgtaagtcga gcagtgtgat gtccactgca gtgtgttgct gggaacagtt aatgagcaaa 60ttgtatacaa tggctagtac attgaccggg atttgttgaa gctggtgagt gttatgactt 120agcctgttag actagtctat gcacatggct ctggtcaact accgctctct catttctcca 180gataaatccc ccatgcttta tattctcttc caaacatact atcctcatca ccacatagtt 240cctttgttaa tgctttgttc tagactttcc cttttctgtt ttcttattca aacctatatc 300tctttgcata gattgtaaat tcaaatgccc tcagggtgca ggcagttcat gtaagggagg 360gaggctagcc agtgagatct gcatcacact gctcgactta ca 402 144 224 DNA Homo sapien 144tcgggtgatg cctcctcagg ccaagaagat aaagcttcag acccctaaca catttccaaa 60aaggaagaaa ggagaaaaaa gggcatcatc cccgttccga agggtcaggg aggaggaaat 120tgaggtggat tcacgagttg cggacaactc ctttgatgcc aagcgaggtg cagccggaga 180ctggggagag cgagccaatc aggttttgaa gttcctctca gtgc 224 145 111 DNA Homo sapien 145agccatttac cacccatcca caaaaaaaaa aaaaaaaaag aaaaatatca aggaataaaa 60atagactttg aacaaaaagg aacatttgct ggcctgagga ggcatcaccc g 111 146 585 DNA Homo sapien 146tagcatgttg agcccagaca cttgtagaga gaggaggaca gttagaagaa gaagaaaagt 60ttttaaatgc tgaaagttac tataagaaag ctttggcttt ggatgagact tttaaagatg 120cagaggatgc tttgcagaaa cttcataaat atatgcaggt gattccttat ttcctcctag 180aaatttagtg atatttgaaa taatgcccaa acttaatttt ctcctgagga aaactattct 240acattactta agtaaggcat tatgaaaagt ttctttttag gtatagtttt tcctaattgg 300gtttgacatt gcttcatagt gcctctgttt ttgtccataa tcgaaagtaa agatagctgt 360gagaaaacta ttacctaaat ttggtatgtt gttttgagaa atgtccttat agggagctca 420cctggtggtt tttaaattat tgttgctact ataattgagc taattataaa aacctttttg 480agacatattt taaattgtct tttcctgtaa tactgatgat gatgttttct catgcatttt 540cttctgaatt gggaccattg ctgctgtgtc tgggctcaca tgcta 585 147 579 DNA Homo sapien misc_feature (1)...(579) n = A,T,C or G 147tagcatgttg agcccagaca ctgggcagcg ggggtggcca cggcagctcc tgccgagccc 60aagcgtgttt gtctgtgaag gaccctgacg tcacctgcca ggctagggag gggtcaatgt 120ggagtgaatg ttcaccgact ttcgcaggag tgtgcagaag ccaggtgcaa cttggtttgc 180ttgtgttcat cacccctcaa gatatgcaca ctgctttcca aataaagcat caactgtcat 240ctccagatgg ggaagacttt ttctccaacc agcaggcagg tccccatcca ctcagacacc 300agcacgtcca ccttctcggg cagcaccacg tcctccacct tctgctggta cacggtgatg 360atgtcagcaa agccgttctg cangaccagc tgccccgtgt gctgtgccat ctcactggcc 420tccaccgcgt acaccgctct aggccgcgca tantgtgcac agaanaaatg atgatccagt 480cccacagccc acgtccaaga ngactttatc cgtcagggat tctttattct gcaggatgac 540ctgtggtatt aattgttcgt gtctgggctc aacatgcta 579 148 249 DNA Homo sapien 148tgacaccttg tccagcatct gcaagccagg aagagagtcc tcaccaagat ccccaccccg 60ttggcaccag gatcttggac ttccaatctc cagaactgtg agaaataagt atttgtcgct 120aaataaatct ttgtggtttc agatatttag ctatagcaga tcaggctgac taagagaaac 180cccataagag ttacatactc attaatctcc gtctctatcc ccaggtctca gatgctggac 240aaggtgtca 249 149 255 DNA Homo sapien 149tgacaccttg tccagcatct gctattttgt gactttttaa taatagccat tctgactggt 60gtgagatggt aactcattgt gggtttggtc tgcatttctc taatgatcag tgatattaag 120ctttttttaa atatgcttgt tgaccacatg tatatcatct tttgagaagt gtctgttcat 180atcctttgcc cactttttaa tttttttatc ttgtaaattt gtttaatttc cttacagatg 240ctggacaagg tgtca 255 150 318 DNA Homo sapien 150ttacgctgca acactgtgga ggccaagctg ggatcacttc ttcattctaa ctggagagga 60gggaagttca agtccagcag agggtgggtg ggtagacagt ggcactcaga aatgtcagct 120ggacccctgt ccccgcatag gcaggacagc aaggctgtgg ctctccaggg ccagctgaag 180aacaggacac tgtctccgct gccacaaagc gtcagagact cccatctttg aagcacggcc 240ttcttggtct tcctgcactt ccctgttctg ttagagacct ggttatagac aaggcttctc 300cacagtgttg cagcgtaa 318 151 323 DNA Homo sapien misc_feature (1)...(323) n = A,T,C or G 151tnacgcngcn acnntgtaga ganggnaagg cnttccccac attncccctt catnanagaa 60ttattcnacc aagnntgacc natgccnttt atgacttaca tgcnnactnc ntaatctgtn 120tcnngcctta aaagcnnntc cactacatgc ntcancactg tntgtgtnac ntcatnaact 180gtcngnaata ggggcncata actacagaaa tgcanttcat actgcttcca ntgccatcng 240cgtgtggcct tncctactct tcttntattc caagtagcat ctctggantg cttccccact 300ctccacattg ttgcagcnat aat 323 152 311 DNA Homo sapien 152tcaagattcc ataggctgac cagtccaagg agagttgaaa tcatgaagga gagtctatct 60ggagagagct gtagttttga gggttgcaaa gacttaggat ggagttggtg ggtgtggtta 120gtctctaagg ttgattttgt tcataaattt catgccctga atgccttgct tgcctcaccc 180tggtccaagc cttagtgaac acctaaaagt ctctgtcttc ttgctctcca aacttctcct 240gaggatttcc tcagattgtc tacattcaga tcgaagccag ttggcaaaca agatgcagtc 300cagagggtca g 311 153 332 DNA Homo sapien 153caagattcca taggctgacc aggaggctat tcaagatctc tggcagttga ggaagtctct 60ttaagaaaat agtttaaaca atttgttaaa atttttctgt cttacttcat ttctgtagca 120gttgatatct ggctgtcctt tttataatgc agagtgggaa ctttccctac catgtttgat 180aaatgttgtc caggctccat tgccaataat gtgttgtcca aaatgcctgt ttagttttta 240aagacggaac tccacccttt gcttggtctt aagtatgtat ggaatgttat gataggacat 300agtagtagcg gtggtcagcc tatggaatct tg 332 154 345 DNA Homo sapien misc_feature (1)...(345) n = A,T,C or G 154tcaagattcc ataggctgac ctggacagag atctcctggg tctggcccag gacagcaggc 60tcaagctcag tggagaaggt ttccatgacc ctcagattcc cccaaacctt ggattgggtg 120acattgcatc tcctcagaga gggaggagat gtangtctgg gcttccacag ggacctggta 180ttttaggatc agggtaccgc tggcctgagg cttggatcat tcanagcctg ggggtggaat 240ggctggcagc ctgtggcccc attgaaatag gctctggggc actccctctg ttcctanttg 300aacttgggta aggaacagga atgtggtcan cctatggaat cttga 345 155 295 DNA Homo sapien misc_feature (1)...(295) n = A,T,C or G 155gacgcttggc cacttgacac attaaacagt tttgcataat cactancatg tatttctagt 60ttgctgtctg ctgtgatgcc ctgccctgat tctctggcgt taatgatggc aagcataatc 120aaacgctgtt ctgttaattc caagttataa ctggcattga ttaaagcatt atctttcaca 180actaaactgt tcttcatana acagcccata ttattatcaa attaagagac aatgtattcc 240aatatccttt anggccaata tatttnatgt cccttaatta agagctactg tccgt 295 156 406 DNA Homo sapien misc_feature (1)...(406) n = A,T,C or G 156gacgcttggc cacttgacac tgcagtggga aaaccagcat gagccgctgc ccccaaggaa 60cctcgaagcc caggcagagg accagccatc ccagcctgca ggtaaagtgt gtcacctgtc 120aggtgggctt ggggtgagtg ggtgggggaa gtgtgtgtgc aaagggggtg tnaatgtnta 180tgcgtgtgag catgagtgat ggctagtgtg actgcatgtc agggagtgtg aacaagcgtg 240cgggggtgtg tgtgcaagtg cgtatgcata tgagaatatg tgtctgtgga tgagtgcatt 300tgaaagtctg tgtgtgtgcg tgtggtcatg anggtaantt antgactgcg caggatgtgt 360gagtgtgcat ggaacactca ntgtgtgtgt caagtggccn ancgtc 406 157 208 DNA Homo sapien misc_feature (1)...(208) n = A,T,C or G 157tgacgcttgg ccacttgaca cactaaaggg tgttactcat cactttcttc tctcctcggt 60ggcatgtgag tgcatctatt cacttggcac tcatttgttt ggcagtgact gtaanccana 120tctgatgcat acaccagctt gtaaattgaa taaatgtctc taatactatg tgctcacaat 180anggtanggg tgaggagaag gggagaga 208 158 547 DNA Homo sapien misc_feature (1)...(547) n = A,T,C or G 158cttcaacctc cttcaacctc cttcaacctc ctggattcaa acaatcatcc cacctcagac 60tccttagtag ctgagactac agactcacgc cactacatct ggctaaattt ttgtagagat 120agggtttcat catgttgccc tggctggtct caaactcctg acctcaagca atgtgcccac 180ctcagcctcc caaagtgctg ggattacagg cataagccac catgcccagt ccatntttaa 240tctttcctac cacattctta ccacactttc ttttatgttt agatacataa atgcttacca 300ttatgataca attgcccaca gtattaagac agtaacatgc tgcacaggtt tgtagcctag 360gaacagtagg caataccaca tagcttaggt gtgtggtaga ctataccatc taggtttgtg 420taagttacac tttatgctgt ttacacaatg acaaaaccat ctaatgatgc atttctcaga 480atgtatcctt gtcagtaagc tatgatgtac agggaacact gcccaaggac acagatattg 540tacctgt 547 159 203 DNA Homo sapien 159gctcctcttg ccttaccaac tcacccagta tgtcagcaat tttatcrgct ttacctacga 60aacagcctgt atccaaacac ttaacacact cacctgaaaa gttcaggcaa caatcgcctt 120ctcatgggtc tctctgctcc agttctgaac ctttctcttt tcctagaaca tgcatttarg 180tcgatagaag ttcctctcag tgc 203 160 402 DNA Homo sapien 160tgtaagtcga gcagtgtgat gggtggaaca gggttgtaag cagtaattgc aaactgtatt 60taaacaataa taataatatt tagcatttat agagcacttt atatcttcaa agtacttgca 120aacattayct aattaaatac cctctctgat tataatctgg atacaaatgc acttaaactc 180aggacagggt catgagaraa gtatgcattt gaaagttggt gctagctatg ctttaaaaac 240ctatacaatg atgggraagt tagagttcag attctgttgg actgtttttg tgcatttcag 300ttcagcctga tggcagaatt agatcatatc tgcactcgat gactytgctt gataacttat 360cactgaaatc tgagtgttga tcatcacact gctcgactta ca 402 161 193 DNA Homo sapien 161agcatgttga gcccagacac tgaccaggag aaaaaccaac caatagaaac acgcccagac 60actgaccagg agaaaaacca accaataaaa acaggcccgg acataagaca aataataaaa 120ttagcggaca aggacatgaa aacagctatt gtaagagcgg atatagtggt gtgtgtctgg 180gctcaacatg cta 193 162 147 DNA Homo sapien 162tgttgagccc agacactgac caggagaaaa accaaccaat aaaaacaggc ccggacataa 60gacaaataat aaaattagcg gacaaggaca tgaaaacagc tattgtaaga gcggatatag 120tggtgtgtgt ctgggctcaa catgcta 147 163 294 DNA Homo sapien 163tagcatgttg agcccagaca caaatctttc cttaagcaat aaatcatttc tgcatatgtt 60tttaaaacca cagctaagcc atgattattc aaaaggacta ttgtattggg tattttgatt 120tgggttctta tctccctcac attatcttca tttctatcat tgacctctta tcccagagac 180tctcaaactt ttatgttata caaatcacat tctgtctcaa aaaatatctc acccacttct 240cttctgtttc tgcgtgtgta tgtgtgtgtg tgtgtgtctg ggctcaacat gcta 294 164 412 DNA Homo sapien misc_feature (1)...(412) n = A,T,C or G 164cgggattggc tttgagctgc agatgctgcc tgtgaccgca cccggcgtgg aacagaaagc 60cacctggctg caagtgcgcc agagccgccc tgactacgtg ctgctgtggg gctggggcgt 120gatgaactcc accgccctga aggaagccca ggccaccgga tacccccgcg acaagatgta 180cggcgtgtgg tgggccggtg cggagcccga tgtgcgtgac gtgggcgaag gcgccaaggg 240ctacaacgcg ctggctctga acggctacgg cacgcagtcc aaggtgatcc angacatcct 300gaaacacgtg cacgacaagg gccagggcac ggggcccaaa gacgaagtgg gctcggtgct 360gtacacccgc ggcgtgatca tccagatgct ggacaaggtg tcaatcacta at 412 165 361 DNA Homo sapien 165ttgacacctt gtccagcatc tgcatctgat gagagcctca gatggctacc actaatggca 60gaaggcaaag gagaacaggc attgtatggc aagaaaggaa gaaagagaga ggggagaaag 120gtgctaggtt cttttcaaca accagttctt gatggaactg agagtaagag ctcaaggcca 180ggtgtggtga ctccaaccag taatcccaac attttaggag gctgaggcag gcagatgtct 240tgaccccatg agtttgtgac cagcctgaac aacatcatga gactccatct ctacaataat 300tacaaaaatt aatcaggcat tgtggtatgc cctgtagtcc cagatgctgg acaaggtgtc 360a 361 166 427 DNA Homo sapien 166twgactgact catgtcccct acacccaact atcttctcca ggtggccagg catgatagaa 60tctgatcctg acttagggga atattttctt tttacttccc atcttgattc cctgccggtg 120agtttcctgg ttcagggtaa gaaaggagct caggccaaag taatgaacaa atccatcctc 180acagacgtac agaataagag aacwtggacw tagccagcag aacmcaaktg aaamcagaac 240mcttamctag gatracaamc mcrraratar ktgcycmcmc wtataataga aaccaaactt 300gtatctaatt aaatatttat ccacygtcag ggcattagtg gttttgataa atacgctttg 360gctaggattc ctgaggttag aatggaaraa caattgcamc gagggtaggg gacatgagtc 420aktctaa 427 167 500 DNA Homo sapien misc_feature (1)...(500) n = A,T,C or G 167aacgtcgcat gctcccggcc gccatggccg cgggatagac tgactcatgt cccctaagat 60agaggagaca cctgctaggt gtaaggagaa gatggttagg tctacggagg ctccagggtg 120ggagtagttc cctgctaagg gagggtagac tgttcaacct gttcctgctc cggcctccac 180tatagcagat gcgagcagga gtaggagaga gggaggtaag agtcagaagc ttatgttgtt 240tatgcgggga aacgccrtat cgggggcagc cragttatta ggggacantr tagwyartcw 300agntagcatc caaagcgngg gagttntccc atatggttgg acctgcaggc ggccgcatta 360gtgattagca tgtgagcccc agacacgcat agcaacaagg acctaaactc agatcctgtg 420ctgattactt aacatgaatt attgtattta tttaacaact ttgagttatg aggcatatta 480ttaggtccat attacctgga 500 168 358 DNA Homo sapien 168ttcatcgctc ggtgactcaa gcctgtaatc ccagaacttt gggaggccga ggggagcaga 60tcacctgagg ttgggagttt gagaccagcc tggccaacat ggtgacaacc cgtctctgct 120aaaaatacaa aaattagcca agcatggtgg catgcacttg taatcccagc tactcgggag 180gctgaggcag gagaatcact tgaggccagg aggcagaggt tgcagtgagg cagaggttga 240gatcatgcca ctgcactcca gcctgggcaa cagagtaaga ctccatctca aaaaaaaaaa 300aaaaaaagaa tgatcagagc cacaaataca gaaaaccttg agtcaccgag cgatgaaa 358 169 1265 DNA Homo sapien 169ttctgtccac accaatctta gagctctgaa agaatttgtc tttaaatatc ttttaatagt 60aacatgtatt ttatggacca aattgacatt ttcgactatt ttttcccaaa aaaagtcagg 120tgaatttcag cacactgagt tgggaatttc ttatcccaga agwcggcacg agcaatttca 180tatttattta agattgattc catactccgt tttcaaggag aatccctgca gtctccttaa 240aggtagaaca aatactttct attttttttt caccattgtg ggattggact ttaagaggtg 300actctaaaaa aacagagaac aaatatgtct cagttgtatt aagcacggac ccatattatc 360atattcactt aaaaaaatga tttcctgtgc accttttggc aacttctctt ttcaatgtag 420ggaaaaactt agtcaccctg aaaacccaca aaataaataa aacttgtaga tgtgggcaga 480argtttgggg gtggacattg tatgtgttta aattaaaccc tgtatcactg agaagctgtt 540gtatgggtca gagaaaatga atgcttagaa gctgttcaca tcttcaagag cagaagcaaa 600ccacatgtct cagctatatt attatttatt ttttatgcat aaagtgaatc atttcttctg 660tattaatttc caaagggttt taccctctat ttaaatgctt tgaaaaacag tgcattgaca 720atgggttgat atttttcttt aaaagaaaaa tataattatg aaagccaaga taatctgaag 780cctgttttat tttaaaactt tttatgttct gtggttgatg ttgtttgttt gtttgtttct 840attttgttgg ttttttactt tgttttttgt tttgttttgt tttggtttdg catactacat 900gcagtttctt taaccaatgt ctgtttggct aatgtaatta aagttgttaa tttatatgag 960tgcatttcaa ctatgtcaat ggtttcttaa tatttattgt gtagaagtac tggtaatttt 1020tttatttaca atatgtttaa agagataaca gtttgatatg ttttcatgtg tttatagcag 1080aagttattta tttctatggc attccagcgg atattttggt gtttgcgagg catgcagtca 1140atattttgta cagttagtgg acagtattca gcaacgcctg atagcttctt tggccttatg 1200ttaaataaaa agacctgttt gggatgtaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1260aaaaa 1265 170 383 DNA Homo sapien 170tgtaagtcga gcagtgtgat gacgatattc ttcttattaa tgtggtaatt gaacaaatga 60tctgtgatac tgatcctgag ctaggaggcg ctgttcagtt aatgggactt cttcgtactc 120taattgatcc agagaacatg ctggctacaa ctaataaaac cgaaaaaagt gaatttctaa 180attttttcta caaccattgt atgcatgttc tcacagcacc acttttgacc aatacttcag 240aagacaaatg tgaaaaggat aatatagttg gatcaaacaa aaacaacaca atttgtcccg 300ataattatca aacagcacag ctacttgcct taattttaga gttactcaca ttttgtgtgg 360aacatcacac tgctcgactt aca 383 171 383 DNA Homo sapien 171tgggcacctt caatatcgca agttaaaaat aatgttgagt ttattatact tttgacctgt 60ttagctcaac agggtgaagg catgtaaaga atgtggactt ctgaggaatt ttcttttaaa 120aagaacataa tgaagtaaca ttttaattac tcaaggacta cttttggttg aagtttataa 180tctagatacc tctacttttt gtttttgctg ttcgacagtt cacaaagacc ttcagcaatt 240tacagggtaa aatcgttgaa gtagtggagg tgaaactgaa atttaaaatt attctgtaaa 300tactataggg aaagaggctg agcttagaat cttttggttg ttcatgtgtt ctgtgctctt 360atcatcacac tgctcgactt aca 383 172 699 DNA Homo sapien misc_feature (1)...(699) n = A,T,C or G 172tcgggtgatg cctcctcagg cttgtcgtta gtgtacacag agctgctcat gaagcgacag 60cggctgcccc tggcacttca gaacctcttc ctctacactt ttggtgcgct tctgaatcta 120ggtctgcatg ctggcggcgg ctctggccca ggcctcctgg aaagtttctc aggatgggca 180gcactcgtgg tgctgagcca ggcactaaat ggactgctca tgtctgctgt catggagcat 240ggcagcagca tcacacgcct ctttgtggtg tcctgctcgc tggtggtcaa cgccgtgctc 300tcagcagtcc tgctacggct gcagctcaca gccgccttct tcctggccac attgctcatt 360ggcctggcca tgcgcctgta ctatggcagc cgctagtccc tgacaacttc caccctgatt 420ccggaccctg tagattgggc gccaccacca gatccccctc ccaggccttc ctccctctcc 480catcagcggc cctgtaacaa gtgccttgtg agaaaagctg gagaagtgag ggcagccagg 540ttattctctg gaggttggtg gatgaagggg tacccctagg agatgtgaag tgtgggtttg 600gttaaggaaa tgcttaccat cccccacccc caaccaagtt nttccagact aaagaattaa 660ggtaacatca atacctaggc ctgaggaggc atcacccga 699 173 701 DNA Homo sapien 173tcgggtgatg cctcctcagg ccagatcaaa cttggggttg aaaactgtgc aaagaaatca 60atgtcggaga aagaattttg caaaagaaaa atgcctaatc agtactaatt taataggtca 120cattagcagt ggaagaagaa atgttgatat tttatgtcag ctattttata atcaccagag 180tgcttagctt catgtaagcc atctcgtatt cattagaaat aagaacaatt ttattcgtcg 240gaaagaactt ttcaatttat agcatcttaa ttgctcagga ttttaaattt tgataaagaa 300agctccactt ttggcaggag tagggggcag ggagagagga ggctccatcc acaaggacag 360agacaccagg gccagtaggg tagctggtgg ctggatcagt cacaacggac tgacttatgc 420catgagaaga aacaacctcc aaatctcagt tgcttaatac aacacaagct catttcttgc 480tcacgttaca tgtcctatgt agatcaacag caggtgactc agggacccag gctccatctc 540catatgagct tccatagtca ccaggacacg ggctctgaaa gtgtcctcca tgcagggaca 600catgcctctt cctttcattg ggcagagcaa gtcacttatg gccagaagtc acactgcagg 660gcagtgccat cctgctgtat gcctgaggag gcatcacccg a 701 174 700 DNA Homo sapien misc_feature (1)...(700) n = A,T,C or G 174tcgggtgatg cctcctcang cccctaaatc agagtccagg gtcagagcca caggagacag 60ggaaagacat agattttaac cggccccctt caggagattc tgaggctcag ttcactttgt 120tgcagtttga acagaggcag caaggctagt ggttaggggc acggtctcta aagctgcact 180gcctggatct gcctcccagc tctgccagga accagctgcg tggccttgag ctgctgacac 240gcagaaagcc ccctgtggac ccagtctcct cgtctgtaag atgaggacag gactctagga 300accctttccc ttggtttggc ctcactttca caggctccca tcttgaactc tatctactct 360tttcctgaaa ccttgtaaaa gaaaaaagtg ctagcctggg caacatggca aaaccctgtc 420tctacaaaaa atacaaaaat tagttgggtg tggtggcatg tgcctgtagt cccagccact 480tgggaggtgc tgaggtggga ggatcacttg agcccgggag gtggaggttg cagtgagcca 540agatcatgcc actgcactcc agcctgagta atagagtaag actctgtctc aaaaacaaca 600acaacaacag tgagtgtgcc tctgtttccg ggttggatgg ggcaccacat ttatgcatct 660ctcagatttg gacgctgcag cctgaggagg catcacccga 700 175 484 DNA Homo sapien misc_feature (1)...(484) n = A,T,C or G 175tatagggcga attgggcccg agttgcatgn tcccggccgc catggccgcg ggattcgggt 60gatgcctcct caggcttgtc tgccacaagc tacttctctg agctcagaaa gtgccccttg 120atgagggaaa atgtcctact gcactgcgaa tttctcagtt ccattttacc tcccagtcct 180ccttctaaac cagttaataa attcattcca caagtattta ctgattacct gcttgtgcca 240gggactattc tcaggctgaa gaaggtggga ggggagggcg gaacctgagg agccacctga 300gccagcttta tatttcaacc atggctggcc catctgagag catctcccca ctctcgccaa 360cctatcgggg catagcccag ggatgccccc aggcggccca ggttagatgc gtccctttgg 420cttgtcagtg atgacataca ccttagctgc ttagctggtg ctggcctgag gaggcatcac 480ccga 484 176 432 DNA Homo sapien 176tcgggtgatg cctcctcagg gctcaaggga tgagaagtga cttctttctg gagggaccgt 60tcatgccacc caggatgaaa atggataggg acccacttgg aggacttgct gatatgtttg 120gacaaatgcc aggtagcgga attggtactg gtccaggagt tatccaggat agattttcac 180ccaccatggg acgtcatcgt tcaaatcaac tcttcaatgg ccatggggga cacatcatgc 240ctcccacaca atcgcagttt ggagagatgg gaggcaagtt tatgaaaagc caggggctaa 300gccagctcta ccataaccag agtcagggac tcttatccca gctgcaagga cagtcgaagg 360atatgccacc tcggttttct aagaaaggac agcttaatgc agatgagatt agcctgagga 420ggcatcaccc ga 432 177 788 DNA Homo sapien 177tagcatgttg agcccagaca cagtagcatt tgtgccaatt tctggttgga atggtgacaa 60catgctggag ccaagtgcta acatgccttg gttcaaggga tggaaagtca cccgtaagga 120tggcaatgcc agtggaacca cgctgcttga ggctctggac tgcatcctac caccaactcg 180cccaactgac aagcccttgc gcctgcctct ccaggatgtc tacaaaattg gtggtattgg 240tactgttcct gttggccgag tggagactgg tgttctcaaa cccggtatgg tggtcacctt 300tgctccagtc aacgttacaa cggaagtaaa atctgtcgaa atgcaccatg aagctttgag 360tgaagctctt cctggggaca atgtgggctt caatgtcaag aatgtgtctg tcaaggatgt 420tcgtcgtggc aacgttgctg gtgacagcaa aaatgaccca ccaatggaag cagctggctt 480cactgctcag gtgattatcc tgaaccatcc aggccaaata agtgccggct atgcccctgt 540attggattgc cacacggctc acattgcatg caagtttgct gagctgaagg aaaagattga 600tcgccgttct ggtaaaaagc tggaagatgg ccctaaattc ttgaagtctg gtgatgctgc 660cattgttgat atggttcctg gcaagcccat gtgtgttgag agcttctcag actatccacc 720tttgggtcgc tttgctgttc gtgatatgag acagacagtt gcggtgggtg tctgggctca 780acatgcta 788 178 786 DNA Homo sapien 178tagcatgttg agcccagaca cctgtgtttc tgggagctct ggcagtggcg gattcatagg 60cacttgggct gcactttgaa tgacacactt ggctttatta gattcactag tttttaaaaa 120attgttgttc gtttcttttc attaaaggtt taatcagaca gatcagacag cataattttg 180tatttaatga cagaaacgtt ggtacatttc ttcatgaatg agcttgcatt ctgaagcaag 240agcctacaaa aggcacttgt tataaatgaa agttctggct ctagaggcca gtactctgga 300gtttcagagc agccagtgat tgttccagtc agtgatgcct agttatatag aggaggagta 360cactgtgcac tcttctaggt gtaagggtat gcaactttgg atcttaaaat tctgtacaca 420tacacacttt atatatatgt atgtatgtat gaaaacatga aattagtttg tcaaatatgt 480gtgtgtttag tattttagct tagtgcaact atttccacat tatttattaa attgatctaa 540gacactttct tgttgacacc ttgaatatta atgttcaagg gtgcaatgtg tattccttta 600gattgttaaa gcttaattac tatgatttgt agtaaattaa cttttaaaat gtatttgagc 660ccttctgtag tgtcgtaggg ctcttacagg gtgggaaaga ttttaatttt ccagttgcta 720attgaacagt atggcctcat tatatatttt gatttatagg agtttgtgtc tgggctcaac 780atgcta 786 179 796 DNA Homo sapien 179tagcatgttg agcccagaca ctggttacaa gaccagacct gcttcctcca tatgtaaaca 60gcttttaaaa agccagtgaa cctttttaat actttggcaa ccttctttca caggcaaaga 120acacccccat ccgccccttg tttggagtgc agagtttggc tttggttctt tgccttgcct 180ggagtatact tctaattcct gttgtcctgc acaagctgaa taccgagcta cccaccgcca 240cccaggccag gtttccactc atttattact ttatgtttct gttccattgc tggtccacag 300aaataagttt tcctttggag gaatgtgatt ataccccttt aatttcctcc ttttgctttt 360ttttaatatc attggtatgt gtttggccca gaggaaactg aaattcacca tcatcttgac 420tggcaatccc attaccatgc tttttttaaa aaacgtaatt tttcttgcct tacattggca 480gagtagccct tcctggctac tggcttaatg tagtcactca gtttctaggt ggcattaggc 540atgagacctg aagcacagac tgtcttacca caaaaggtga caagatctca aaccttagcc 600aaagggctat gtcaggtttc aatgctatct gcttctgttc ctgctcactg ttctggattt 660tgtccttctt catccctagc accagaattt cccagtctcc ctccctacct tcccttgttt 720taattctaat ctatcagcaa aataactttt caaatgtttt aaccggtatc tccatgtgtc 780tgggctcaac atgcta 796 180 488 DNA Homo sapien 180ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt 60aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattggg cccgacgtcg 120catgctcccg gccgccatgg ccgcgggata gcatgttgag cccagacacc tgcaggtcat 180ttggagagat ttttcacgtt accagcttga tggtcttttt caggaggaga gacactgagc 240actcccaagg tgaggttgaa gatttcctct agatagccgg ataagaagac taggagggat 300gcctagaaaa tgattagcat gcaaatttct acctgccatt tcagaactgt gtgtcagccc 360acattcagct gcttcttgtg aactgaaaag agagaggtat tgagactttt ctgatggccg 420ctctaacatt gtaacacagt aatctgtgtg tgtgtgggtg tgtgtgtgtg tctgggctca 480acatgcta 488 181 317 DNA Homo sapien 181tagcatgttg agcccagaca cggcgacggt acctgatgag tggggtgatg gcacctgtga 60aaaggaggaa cgtcatcccc catgatattg gggacccaga tgatgaacca tggctccgcg 120tcaatgcata tttaatccat gatactgctg attggaagga cctgaacctg aagtttgtgc 180tgcaggttta tcgggactat tacctcacgg gtgatcaaaa cttcctgaag gacatgtggc 240ctgtgtgtct agtaagggat gcacatgcag tggccagtgt gccaggggta tggttggtgt 300ctgggctcaa catgcta 317 182 507 DNA Homo sapien misc_feature (1)...(507) n = A,T,C or G 182tagcatgttg agcccagaca ctggctgtta gccaaatcct ctctcagctg ctccctgtgg 60tttggtgact caggattaca gaggcatcct gtttcaggga acaaaaagat tttagctgcc 120agcagagagc accacataca ttagaatggt aaggactgcc acctccttca agaacaggag 180tgagggtggt ggtgaatggg aatggaagcc tgcattccct gatgcatttg tgctctctca 240aatcctgtct tagtcttagg aaaggaagta aagtttcaag gacggttccg aactgctttt 300tgtgtctggg ctcaacatgc tatcccgcgg ccatggcggc cgggagcatg cgacgtcggg 360cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt 420gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttccca 480gctggcgtaa tancgaaaag gcccgca 507 183 227 DNA Homo sapien 183gatttacgct gcaacactgt ggaggtagcc ctggagcaag gcaggcatgg atgcttctgc 60aatccccaaa tggagcctgg tatttcagcc aggaatctga gcagagcccc ctctaattgt 120agcaatgata agttattctc tttgttcttc aaccttccaa tagccttgag cttccagggg 180agtgtcgtta atcattacag cctggtctcc acagtgttgc agcgtaa 227 184 225 DNA Homo sapien 184ttacgctgca acactgtgga gcagattaac atcagacttt tctatcaaca tgactggggt 60tactaaaaag acaacaaatc aatggcttca aaagtctaag gaataatttc gatacttcaa 120ctttataaaa cctgacaaaa ctatcaatca agcataaaga cagatgaaga acatttccag 180attttggcca atcagatatt ttacctccac agtgttgcag cgtaa 225 185 597 DNA Homo sapien 185ggcccgacgt cgcatgctcc cggccgccat ggccgcggga ttcgttaggg tctctatcca 60ctgggaccca taggctagtc agagtattta gagttgagtt cctttctgct tcccagaatt 120tgaaagaaaa ggagtgaggt gatagagctg agagatcaga tttgcctctg aagcctgttc 180aagatgtatg tgctcagacc ccaccactgg ggcctgtggg tgaggtcctg ggcatctatt 240tgaatgaatt gctgaagggg agcactatgc caaggaaggg gaacccatcc tggcactggc 300acaggggtca ccttatccag tgctcagtgc ttctttgctg ctacctggtt ttctctcata 360tgtgaggggc aggtaagaag aagtgcccrg tgttgtgcga gttttagaac atctaccagt 420aagtggggaa gtttcacaaa gcagcagctt tgttttgtgt attttcacct tcagttagaa 480gaggaaggct gtgagatgaa tgttagttga gtggaaaaga cgggtaagct tagtggatag 540agaccctaac gaatcactag tgcggccgcc ttgcaggtcg accatatggg agagctc 597 186 597 DNA Homo sapien 186ggcccgaagt tgcatgttcc cggccgccat ggccgcggga ttcgttaggg tctctatcca 60ctacctaaaa aatcccaaac atataactga actcctcaca cccaattgga ccaatccatc 120accccagagg cctacagatc ctcctttgat acataagaaa atttccccaa actacctaac 180tatatcattt tgcaagattt gttttaccaa attttgatgg cctttctgag cttgtcagtg 240tgaaccacta ttacgaacga tcggatatta actgcccctc accgtccagg tgtagctggc 300aacatcaagt gcagtaaata ttcattaagt tttcacctac taaggtgctt aaacacccta 360gggtgccatg tcggtagcag atcttttgat ttgtttttat ttcccataag ggtcctgttc 420aaggtcaatc atacatgtag tgtgagcagc tagtcactat cgcatgactt ggagggtgat 480aatagaggcc tcctttgctg ttaaagaact cttgtcccag cctgtcaaag tggatagaga 540ccctaacgaa tcactagtgc ggccgcctgc aggtcgacca tatgggagag ctcccaa 597 187 324 DNA Homo sapien 187tcgttagggt ctctatccac ttgcaggtaa aatccaatcc tgtgtatatc ttatagtctt 60ccatatgtag tggttcaaga gactgcagtt ccagaaagac tagccgagcc catccatgtc 120ttccacttaa ccctgctttg ggttacacat cttaactttt ctgttcaagt ttctctgtgt 180agtttatagc atgagtattg ggawaatgcc ctgaaacctg acatgagatc tgggaaacac 240aaacttactc aataagaatt tctcccatat ttttatgatg gaaaaatttc acatgcacag 300aggagtggat agagacccta acga 324 188 178 DNA Homo sapien misc_feature (1)...(178) n = A,T,C or G 188gcgcggggat tcggggtgat acctcctcat gccaaaatac aacgtntaat ttcacaactt 60gccttccaat ttacgcattt tcaatttgct ctccccattt gttgagtcac aacaaacacc 120attgcccaga aacatgtatt acctaacatg cacatactct taaaactact catccctt 178 189 367 DNA Homo sapien 189tgacaccttg tccagcatct gacacagtct tggctcttgg aaaatattgg ataaatgaaa 60atgaatttct ttagcaagtg gtataagctg agaatatacg tatcacatat cctcattcta 120agacacattc agtgtccctg aaattagaat aggacttaca ataagtgtgt tcactttctc 180aatagctgtt attcaattga tggtaggcct taaaagtcaa agaaatgaga gggcatgtga 240aaaaaagctc aacatcactg atcattagaa aacttccatt caaaccccca atgagatacc 300atctcatacc agtcagaatg gctattatta aaaagtcaaa aaataacaga tgctggacaa 360ggtgtca 367 190 369 DNA Homo sapien misc_feature (1)...(369) n = A,T,C or G 190gacaccttgt ccagcatctg acaacgctaa cagcctgagg agatctttat ttatttattt 60agtttttact ctggctaggc agatggtggc taaaacattc atttacccat ttattcattt 120aattgttcct gcaaggccta tggatagagt attgtccagc actgctctgg aagctaggag 180catggggatg aacaagatag gctacatcct gttcccacag aacttccact ttagtctggg 240aaacagatga tatatacaaa tatataaatg aattcaggta gttttaagta cgaaaagaat 300aagaaagcag agtcatgatt tanaatgctg gaaacagggg ctattgcttg agatattgaa 360ggtgcccaa 369 191 369 DNA Homo sapien 191tgacaccttg tccagcatct gcacagggaa aagaaactat tatcagagtg aacaggcaac 60ctacagaatg ggagaaaatt tttgcaatct atccatctga caaagggcta atatccagaa 120tctacaaaga acttatacaa atttacaaga aacaaacaaa caaacaactc ctcaaaaagt 180gggtgaagga tgtgaacaga cacttctcaa aagaagacat ttatggggcc aacaaacata 240tgaaaaaaag ctcatcatca ctggtcacta gataaatgca aatcaaaacc acaatgagat 300accatctcat tccagttaga atggcaatca ttaaaaagtc aggaaacaac agatgctgga 360caaggtgtc 369 192 449 DNA Homo sapien 192tgacgcttgg ccacttgaca cttcatcttt gcacagaaaa acttctttac agatttaatt 60caagactggt ctagtgacag tcctccagac attttttcat ttgttccata tacgtggaat 120tttaaaatca tgtttcatca gtttgaaatg atttgggctg ctaatcaaca caattggatc 180gactgttcta ctaaacaaca ggaaaatgtg tatctggcag cctgtggaga aacactaaac 240attgattttt ctttgccttt tacggacttt gttccagcta catgtaatac caagttctct 300ttaagaggag aagatgttga tcttcatttg tttctaccag actgccaccc tagtaaatat 360tctttattta tgctggtaaa aaattgccat ccaaataaga tgattcatga tactggtatt 420cctgctgagt gtcaagtggc caagcgtca 449 193 372 DNA Homo sapien 193tgacgcttgg ccacttgaca ccagggatgt akcagttgaa tataatcctg caattgtaca 60tattggcaat ttcccatcaa acattctaga aagagacaac caggattgct aggccataaa 120agctgcaata aataactggt aattgcagta atcatttcag gccaattcaa tccagtttgg 180ctcagaggtg cctttggctg agagaagagg tgagatataa tgtgttttct tgcaacttct 240tggaagaata actccacaat agtctgagga ctagatacaa acctatttgc cattaaagca 300ccagagtctg ttaattccag tactgataag tgttggagat tagactccag tgtgtcaagt 360ggccaagcgt ca 372 194 309 DNA Homo sapien misc_feature (1)...(309) n = A,T,C or G 194tgacgcttgg ccacttgaca cttatgtaga atccatcgtg ggctgatgca agccctttat 60ttaggcttag tgttgtgggc accttcaata tcacactaga gacaaacgcc acaagatctg 120cagaaacatt cagttctgan cactcgaatg gcaggataac tttttgtgtt gtaatccttc 180acatatacaa aaacaaactc tgcantctca cgttacaaaa aaacgtactg ctgtaaaata 240ttaagaaggg gtaaaggata ccatctataa caaagtaact tacaactagt gtcaagtggc 300caagcgtca 309 195 312 DNA Homo sapien misc_feature (1)...(312) n = A,T,C or G 195tgacgcttgg ccacttgaca cccaatctcg cacttcatcc tcccagcacc tgatgaagta 60ggactgcaac tatccccact tcccagatga ggggaccaan gtacacatta ggacccggat 120gggagcacag atttgtccga tcccagactc caagcactca gcgtcactcc aggacagcgg 180ctttcagata aggtcacaaa catgaatggc tccgacaacc ggagtcagtc cgtgctgagt 240taaggcaatg gtgacacgga tgcacgtgtn acctgtaatg gttcatcgta agtgtcaagt 300ggccaagcgt ca 312 196 288 DNA Homo sapien 196tgtatcgacg tagtggtctc ctcagccatg cagaactgtg actcaattaa acctctttcc 60tttatgaatt acccaatctc gggtagtgtc tttatagtag tgtgagaatg gactaataca 120agtacatttt acttagtaat aataataaac aaatatatta catttttgtg tatttactac 180accatatttt ttattgttat tgtagtgtac accttctact tattaaaaga aataggcccg 240aggcgggcag atcacgaggt caggagatgg agaccactac gtcgatac 288 197 289 DNA Homo sapien 197ttgggcacct tcaatatcat gacaggtgat gtgataacca agaaggctac taagtgatta 60atgggtgggt aatgtataca gagtaggtac actggacaga ggggtaattc atagccaagg 120caggagaagc agaatggcaa aacatttcat cacactactc aggatagcat gcagtttaaa 180acctataagt agtttatttt tggaattttc cacttaatat tttcagactg caggtaacta 240aactgtggaa cacaagaaca tagataaggg gagaccacta cgtcgatac 289 198 288 DNA Homo sapien 198gtatcgacgt agtggtctcc caagcagtgg gaagaaaacg tgaaccaatt aaaatgtatc 60agatacccca aagaaaggcg cttgagtaaa gattccaagt gggtcacaat ctcagatctt 120aaaattcagg ctgtcaaaga gatttgctat gaggttgctc tcaatgactt caggcacagt 180cggcaggaga ttgaagccct ggccattgtc aagatgaagg agctttgtgc catgtatggc 240aagaaagacc ccaatgagcg ggactcctgg agaccactac gtcgatac 288 199 1027 DNA Homo sapien misc_feature (1)...(1027) n = A,T,C or G 199gctttttggg aaaaacncaa ntgggggaaa gggggnttnn tngcaagggg ataaaggggg 60aancccaggg tttccccatt cagggaggtg taaaaagncg gccaggggat tgtaanagga 120ttcaataata gggggaatgg gcccngaagt tgcaaggttc cngcccgcca tgnccgcggg 180atttagtgac attacgacgs tggtaataaa gtgggsccaa waaatatttg tgatgtgatt 240tttsgaccag tgaacccatt gwacaggacc tcatttccty tgagatgrta gccataatca 300gataaaagrt tagaagtytt tctgcacgtt aacagcatca ttaaatggag tggcatcacc 360aatttcaccc tttgttagcc gataccttcc ccttgaaggc attcaattaa gtgaccaatc 420gtcatacgag aggggatggc atggggattg atgatgatat caggggtgat accttcacag 480gtgaaaggca tatcctcttg tctatactga ataccacaag tacccttttg accatgtcga 540ctagcaaatt tgtctccaat ctgtgtwatc cctaacagag cgtaccctta ttttacaaaa 600tttatatcct tcctgattga gagttaccat aacctgatcc acaatgcccg tctcgctwgt 660tctgagaaaa gtgctacagt ctctcttggt atagcgtcta ttggtgctct ccaattcatc 720ttcatttttc aggcaaggtg aactgttttg cctataataa cmtcatctcc tgatacmcga 780aacccckgga rctatcaaac catcatcatc cagcgttckt watgtymcta aatccctatt 840gcggccgcct gcaggtcaac atatnggaaa accccccacc ccttnggagc ntaccttgaa 900ttttccatat gtcccntaaa ttanctngnc ttancctggc cntaacctnt tccggtttaa 960attgtttccg cccccnttcc ccnccttnna accggaaacc ttaattttna accnggggtt 1020cctatcc 1027 200 207 DNA Homo sapien 200agtgacatta cgacgctggc catcttgaat cctagggcat gaagttgccc caaagttcag 60cacttggtta agcctgatcc ctctggttta tcacaaagaa taggatggga taaagaaagt 120ggacacttaa ataagctata aattatatgg tccttgtcta gcaggagaca actgcacagg 180tatactacca gcgtcgtaat gtcacta 207 201 209 DNA Homo sapien 201tgggcacctt caatatctat taaaagcaca aatactgaag aacacaccaa gactatcaat 60gaggttacat ctggagtcct cgatatatca ggaaaaaatg aagtgaacat tcacagagtt 120ttacttcttt gggaactcaa atgctagaaa agaaaagggt gccctctttc tctggcttcc 180tggtcctatc cagcgtcgta atgtcacta 209 202 349 DNA Homo sapien misc_feature (1)...(349) n = A,T,C or G 202ntacgctgca acactgtgga gccactggtt tttattcccg gcaggttatc cagcaaacag 60tcactgaaca caccgaagac cgtggtatgg taaccgttca cagtaatcgt tccagtcgtc 120tgcgggaccc cgacgagcgt cactgggtac agaccagatt cagccggaag agaaagcgcc 180gcagggagag actcgaactc cactccgctg gtgagcagcc ccatgttttc aactcgaagt 240tcaaacggca ttgggttata taccatcagc tgaacttcac acacatctcc ttgaacccac 300tggaaatcta ttttcttgtt ccgctcttct ccacagtgtt gcagcgtaa 349 203 241 DNA Homo sapien 203tgctcctctt gccttaccaa cccaaagccc actgtgaaat atgaagtgaa tgacaaaatt 60cagttttcaa cgcaatatag tatagtttat ctgattcttt tgatctccag gacactttaa 120acaactgcta ccaccaccac caacctaggg atttaggatt ctccacagac cagaaattat 180ttctcctttg agtttcaggc tcctctggga ctcctgttca tcaatgggtg gtaaatggct 240a 241 204 248 DNA Homo sapien 204tagccattta ccacccatct gcaaaccswg acmwwcargr cywgwackya ggcgatttga 60agtactggta atgctctgat catgttagtt acataagtgt ggtcagttta caaaaattca 120cagaactaaa tactcaatgc tatgtgttca tgtctgtgtt tatgtgtgtg taatgtttca 180attaagtttt tttaaaaaaa agagatgatt tccaaataag aaagccgtgt tggtaaggca 240agaggagc 248 205 505 DNA Homo sapien misc_feature (1)...(505) n = A,T,C or G 205tacgctgcaa cactgtggag ccattcatac aggtccctaa ttaaggaaca agtgattatg 60ctacctttgc acggttaggg taccgcggcc gttaaacatg tgtcactggg caggcggtgc 120ctctaatact ggtgatgcta gaggtgatgt ttttggtaaa caggcggggt aagatttgcc 180gagttccttt tacttttttt aacctttcct tatgagcatg cctgtgttgg gttgacagtg 240ggggtaataa tgacttgttg gttgattgta gatattgggc tgttaattgt cagttcagtg 300ttttaatctg acgcaggctt atgcggagga gaatgttttc atgttactta tactaacatt 360agttcttcta tagggtgata gattggtcca attgggtgtg aggagttcag ttatatgttt 420gggatttttt aggtagtggg tgttganctt gaacgctttc ttaattggtg gctgctttta 480rgcctactat gggtggtaaa tggct 505 206 179 DNA Homo sapien 206tagactgact catgtcccct accaaagccc atgtaaggag ctgagttctt aaagactgaa 60gacagactat tctctggaga aaaataaaat ggaaattgta ctttaaaaaa aaaaaaaatc 120ggccgggcat ggtagcacac acctgtaatc ccagctacta ggggacatga gtcagtcta 179 207 176 DNA Homo sapien 207agactgactc atgtccccta ccccaccttc tgctgtgctg ccgtgttcct aacaggtcac 60agactggtac tggtcagtgg cctgggggtt ggggacctct attatatggg atacaaattt 120aggagttgga attgacacga tttagtgact gatgggatat gggtggtaaa tggcta 176 208 196 DNA Homo sapien 208agactgactc atgtccccta tttaacaggg tctctagtgc tgtgaaaaaa aaaaatgctg 60aacattgcat ataacttata ttgtaagaaa tactgtacaa tgactttatt gcatctgggt 120agctgtaagg catgaaggat gccaagaagt ttaaggaata tgggtggtaa atggctaggg 180gacatgagtc agtcta 196 209 345 DNA Homo sapien misc_feature (1)...(345) n = A,T,C or G 209gacgcttggc cacttgacac cttttatttt ttaaggattc ttaagtcatt tangtnactt 60tgtaagtttt tcctgtgccc ccataagaat gatagcttta aaaattatgc tggggtagca 120aagaagatac ttctagcttt agaatgtgta ggtatagcca ggattcttgt gaggaggggt 180gatttagagc aaatttctta ttctccttgc ctcatctgta acatggggat aataatagaa 240ctggcttgac aaggttggaa ttagtattac atggtaaata catgtaaaat gtttagaatg 300gtgccaagta tctaggaagt acttgggcat gggtggtaaa tggct 345 210 178 DNA Homo sapien 210gacgcttggc cacttgacac tagagtaggg tttggccaac tttttctata aaggaccaga 60gagtaaatat ttcaggcttt gtgggttgtg cagtctctct tgcaactact cagctctgcc 120attgtagcat agaaatcagc catagacagg acagaaatga atgggtggta aatggcta 178 211 454 DNA Homo sapien 211tgggcacctt caatatctat ccagcgcatc taaattcgct tttttcttga ttaaaaattt 60caccacttgc tgtttttgct catgtatacc aagtagcagt ggtgtgaggc catgcttgtt 120ttttgattcg atatcagcac cgtataagag cagtgctttg gccattaatt tatcttcatt 180gtagacagca tagtgtagag tggtatctcc atactcatct ggaatatttg gatcagtgcc 240atgttccagc aacattaacg cacattcatc ttcctggcat tgtacggcct ttgtcagagc 300tgtcctcttt ttgttgtcaa ggacattaag ttgacatcgt ctgtccagca cgagttttac 360tacttctgaa ttcccattgg cagaggccag atgtagagca gtcctctttt gcttgtccct 420cttgttcaca tcagtgtccc tgagcataac ggaa 454 212 337 DNA Homo sapien 212tccgttatgc cacccagaaa acctactgga gttacttatt aacatcaagg ctggaaccta 60tttgcctcag tcctatctga ttcatgagca catggttatt actgatcgca ttgaaaacat 120tgatcacctg ggtttcttta tttatcgact gtgtcatgac aaggaaactt acaaactgca 180acgcagagaa actattaaag gtattcagaa acgtgaagcc agcaattgtt tcgcaattcg 240gcattttgaa aacaaatttg ccgtggaaac tttaatttgt tcttgaacag tcaagaaaaa 300cattattgag gaaaattaat atcacagcat aacggaa 337 213 715 DNA Homo sapien misc_feature (1)...(715) n = A,T,C or G 213tcgggtgatg cctcctcagg catcttccat ccatctcttc aagattagct gtcccaaatg 60tttttccttc tcttctttac tgataaattt ggactccttc ttgacactga tgacagcttt 120agtatccttc ttgtcacctt gcagacttta aacataaaaa tactcattgg ttttaaaagg 180aaaaaagtat acattagcac tattaagctt ggccttgaaa cattttctat cttttattaa 240atgtcggtta gctgaacaga attcatttta caatgcagag tgagaaaaga agggagctat 300atgcatttga gaatgcaagc attgtcaaat aaacatttta aatgctttct taaagtgagc 360acatacagaa atacattaag atattagaaa gtgtttttgc ttgtgtacta ctaattaggg 420aagcaccttg tatagttcct cttctaaaat tgaagtagat tttaaaaacc catgtaattt 480aattgagctc tcagttcaga ttttaggaga attttaacag ggatttggtt ttgtctaaat 540tttgtcaatt tntttagtta atctgtataa ttttataaat gtcaaactgt atttagtccg 600ttttcatgct gctatgaaag aaatacccan gacagggtta tttataaang gaaagangtt 660aatttgactc ccagttcaca ggcctgagga ngnatcnccc gaaatcctta ttgcg 715 214 345 DNA Homo sapien misc_feature (1)...(345) n = A,T,C or G 214ggtaangngc atacntcggt gctccggccg ccggagtcgg gggattcggg tgatgcctcc 60tcaggcccac ttgggcctgc ttttcccaaa tggcagctcc tctggacatg ccattccttc 120tcccacctgc ctgattcttc atatgttggg tgtccctgtt tttctggtgc tatttcctga 180ctgctgttca gctgccactg tcctgcaaag cctgcctttt taaatgcctc accattcctt 240catttgtttc ttaaatatgg gaagtgaaag tgccacctga ggccgggcac agtggctcac 300gcctgtaatc ccagcacttt gggagcctga ggaggcatca cccga 345 215 429 DNA Homo sapien 215ggtgatgcct cctcaggcga agctcaggga ggacagaaac ctcccgtgga gcagaagggc 60aaaagctcgc ttgatcttga ttttcagtac gaatacagac cgtgaaagcg gggcctcacg 120atccttctga ccttttgggt tttaagcagg aggtgtcaga aaagttacca cagggataac 180tggcttgtgg cggccaagcg ttcatagcga cgtcgctttt tgatccttcg atgtcggctc 240ttcctatcat tgtgaagcag aattcaccaa gcgttggatt gttcacccac taatagggaa 300cgtgagctgg gtttagaccg tcgtgagaca ggttagtttt accctactga tgatgtgtkg 360ttgccatggt aatcctgctc agtacgagag gaaccgcagg ttcasacatt tggtgtatgt 420gcttgcctt 429 216 593 DNA Homo sapien misc_feature (1)...(593) n = A,T,C or G 216tgacacctat gtccngcatc tgttcacagt ttccacaaat agccagcctt tggccacctc 60tctgtcctga ggtatacaag tatatcagga ggtgtatacc ttctcttctc ttccccacca 120aagagaacat gcaggctctg gaagctgtct taggagcctt tgggctcaga atttcagagt 180cttgggtacc ttggatgtgg tctggaagga gaaacattgg ctctggataa ggagtacagc 240cggaggaggg tcacagagcc ctcagctcaa gcccctgtgc cttagtctaa aagcagcttt 300ggatgaggaa gcaggttaag taacatacgt aagcgtacac aggtagaaag tgctgggagt 360cagaattgca cagtgtgtag gagtagtacc tcaatcaatg agggcaaatc aactgaaaga 420agaagaccna ttaatgaatt gcttangggg aaggatcaag gctatcatgg agatctttct 480aggaagatta ttgtttanaa ttatgaaagg antagggcag ggacagggcc agaagtanaa 540ganaacattg cctatanccc ttgtcttgca cccagatgct ggacaaggtg tca 593 217 335 DNA Homo sapien 217tgacaccttg tccagcatct gacgtgaaga tgagcagctc agaggaggtg tcctggattt 60cctggttctg tgggctccgt ggcaatgaat tcttctgtga agtggatgaa gactacatcc 120aggacaaatt taatcttact ggactcaatg agcaggtccc tcactatcga caagctctag 180acatgatctt ggacctggag cctgatgaag aactggaaga caaccccaac cagagtgacc 240tgattgagca ggcagccgag atgctttatg gattgatcca cgcccgctac atccttacca 300accgtggcat cgcccagatg ctggacaagg tgtca 335 218 248 DNA Homo sapien 218tacgtactgg tcttgaaggt cttaggtaga gaaaaaatgt gaatatttaa tcaaagacta 60tgtatgaaat gggactgtaa gtacagaggg aagggtggcc cttatcgcca gaagttggta 120gatgcgtccc cgtcatgaaa tgttgtgtca ctgcccgaca tttgccgaat tactgaaatt 180ccgtagaatt agtgcaaatt ctaacgttgt tcatctaaga ttatggttcc atgtttctag 240tactttta 248 219 530 DNA Homo sapien misc_feature (1)...(530) n = A,T,C or G 219tgacgcttgg ccacttgaca caagtagggg ataaggacaa agacccatna ggtggcctgt 60cagccttttg ttactgttgc ttccctgtca ccacggcccc ctctgtaggg gtgtgctgtg 120ctctgtggac attggtgcat tttcacacat accattctct ttctgcttca cagcagtcct 180gaggcgggag cacacaggac taccttgtca gatgangata atgatgtctg gccaactcac 240cccccaacct tctcactagt tatangaaga gccangccta naaccttcta tcctgncccc 300ttgccctatg acctcatccc tgttccatgc cctattctga tttctggtga actttggagc 360agcctggttt ntcctcctca ctccagcctc tctccatacc atggtanggg ggtgctgttc 420cacncaaang gtcaggtgtg tctggggaat cctnananct gccnggagtt tccnangcat 480tcttaaaaac cttcttgcct aatcanatng tgtccagtgg ccaaccntcn 530 220 531 DNA Homo sapien 220tgacgcttgg ccacttgaca ctaaatagca tcttctaaag gcctgattca gagttgtgga 60aaattctccc agtgtcaggg attgtcagga acagggctgc tcctgtgctc actttacctg 120ctgtgtttct gctggaaaag gagggaagag gaatggctga tttttaccta atgtctccca 180gtttttcata ttcttcttgg atcctcttct ctgacaactg ttcccttttg gtcttcttct 240tcttgctcag agagcaggtc tctttaaaac tgagaaggga gaatgagcaa atgattaaag 300aaaacacact tctgaggccc agagatcaaa tattaggtaa atactaaacc gcttgcctgc 360tgtggtcact tttctcctct ttcacatgct ctatccctct atcccccacc tattcatatg 420gcttttatct gccaagttat ccggcctctc atcaaccttc tcccctagcc tactggggga 480tatccatctg ggtctgtctc tggtgtattg gtgtcaagtg gccaagcgtc a 531 221 530 DNA Homo sapien 221attgacgctt ggccacttga cacccgcctg cctgcaatac tggggcaagg gccttcactg 60ctttcctgcc accagctgcc actgcacaca gagatcagaa atgctaccaa ccaagactgt 120tggtcctcag cctctctgag gagaaagagc agaagcctgg aagtcagaag agaagctaga 180tcggctacgg ccttggcagc cagcttcccc acctgtggca ataaagtcgt gcatggctta 240acaatggggg cacctcctga gaaacacatt gttaggcaat tcggcgtgtg ttcatcagag 300catatttaca caaacctcga tagtgcagcc tactatccac tattgctcct acgctgcaaa 360cctgaacagc atgggactgt actgaatact ggaagcagct ggtgatggta cttatttgtg 420tatctaaaca cagagaaggt acagtaagaa tatggtatca taaacttaca gggaccgcca 480tcctatatgc agtctgttgt gaccaaaatg tgtcaagtgg ccaagcgtca 530 222 578 DNA Homo sapien misc_feature (1)...(578) n = A,T,C or G 222tgtatcgacg tagtggtctc cgggctacta ggccgttgtg tgctggtagt acctggttca 60ctgaaaggcg catctccctc cccgcgtcgc cctgaagcag ggggaggact tcgcccagcc 120aaggcagttg tatgagtttt agctgcggca cttcgagacc tctgagccca cctccttcag 180gagccttccc cgattaagga agccagggta aggattcctt cctcccccag acaccacgaa 240caaaccacca ccccccctat tctggcagcc catatacatc agaacgaaac aaaaataaca 300aataaacnaa aaccaaaaaa aaaagagaag gggaaatgta tatgtctgtc catcctgttg 360ctttagcctg tcagctccta nagggcaggg accgtgtctt ccgaatggtc tgtgcagcgc 420cgactgcggg aagtatcgga ggaggaagca gagtcagcag aagttgaacg gtgggcccgg 480cggctcttgg gggctggtgt tgtacttcga gaccgctttc gctttttgtc ttagatttac 540gtttgctctt tggagtggga naccactacn tcnataca 578 223 578 DNA Homo sapien 223tgtatcgacg tagtggtctc ctcttgcaaa ggactggctg gtgaatggtt tccctgaatt 60atggacttac cctaaacata tcttatcatc attaccagtt gcaaaatatt agaatgtgtt 120gtcactgttt catttgattc ctagaaggtt agtcttagat atgttacttt aacctgtatg 180ctgtagtgct ttgaatgcat tttttgtttg catttttgtt tgcccaacct gtcaattata 240gctgcttagg tctggactgt cctggataaa gctgttaaaa tattcaccag tccagccatc 300ttacaagcta attaagtcaa ctaaatgctt ccttgttttg ccagacttgt tatgtcaatc 360ctcaatttct gggttcattt tgggtgccct aaatcttagg gtgtgacttt cttagcatcc 420tgtaacatcc attcccaagc aagcacaact tcacataata ctttccagaa gttcattgct 480gaagcctttc cttcacccag cggagcaact tgattttcta caacttccct catcagagcc 540acaagagtat gggatatgga gaccactacg tcgataca 578 224 345 DNA Homo sapien misc_feature (1)...(345) n = A,T,C or G 224tgtatcgacg tantggtctc ccaaggtgct gggattgcag gcatgagcca ccactcccag 60gtggatcttt ttctttatac ttacttcatt aggtttctgt tattcaagaa gtgtagtggt 120aaaagtcttt tcaatctaca tggttaaata atgatagcct gggaaataaa tagaaatttt 180ttctttcatc tttaggttga ataaagaaac agaaaaaata gaacatactg aaaataatct 240aagttccaac catagaagaa ctgcagaaga aatgaagaaa gtgatgatga tttagatttt 300gatattgatt tagaagacac aggaggagac cactacgtcg ataca 345 225 347 DNA Homo sapien 225tgtatcgacg tagtggtctc caaactgagg tatgtgtgcc actagcacac aaagccttcc 60aacagggacg caggcacagg cagtttaaag ggaatctgtt tctaaattaa tttccacctt 120ctctaagtat tctttcctaa aactgatcaa ggtgtgaagc ctgtgctctt tcccaactcc 180cctttgacaa cagccttcaa ctaacacaag aaaaggcatg tctgacactc ttcctgagtc 240tgactctgat acgttgttct gatgtctaaa gagctccaga acaccaaagg gacaattcag 300aatgctggtg tataacagac tccaatggag accactacgt cgataca 347 226 281 DNA Homo sapien misc_feature (1)...(281) n = A,T,C or G 226aggngnggga ntgtatcgac gtagtggtct cccaacagtc tgtcattcag tctgcaggtg 60tcagtgtttt ggacaatgag gcaccattgt cacttattga ctcctcagct ctaaatgctg 120aaattaaatc ttgtcatgac aagtctggaa ttcctgatga ggttttacaa agtattttgg 180atcaatactc caacaaatca gaaagccaga aagaggatcc tttcaatatt gcagaaccac 240gagtggattt acacacctca ggagaccact acgtcgatac a 281 227 3646 DNA Homo sapien 227gggaaacact tcctcccagc cttgtaaggg ttggagccct ctccagtata tgctgcagaa 60tttttctctc ggtttctcag aggattatgg agtccgcctt aaaaaaggca agctctggac 120actctgcaaa gtagaatggc caaagtttgg agttgagtgg ccccttgaag ggtcactgaa 180cctcacaatt gttcaagctg tgtggcgggt tgttactgaa actcccggcc tccctgatca 240gtttccctac attgatcaat ggctgagttt ggtcaggagc accccttccg tggctccact 300catgcaccat tcataatttt acctccaagg tcctcctgag ccagaccgtg ttttcgcctc 360gaccctcagc cggttcggct cgccctgtac tgcctctctc tgaagaagag gagagtctcc 420ctcacccagt cccaccgcct taaaaccagc ctactccctt agggtcatcc catgtctcct 480cggctatgtc ccctgtaggc tcatcaccca ttgcctcttg gttgcaaccg tggtgggagg 540aagtagcccc tctactacca ctgagagagg cacaagtccc tctgggtgat gagtgctcca 600cccccttcct ggtttatgtc ccttctttct acttctgact tgtataattg gaaaacccat 660aatcctccct tctctgaaaa gccccaggct ttgacctcac tgatggagtc tgtactctgg 720acacattggc ccacctggga tgactgtcaa cagctccttt tgaccctttt cacctctgaa 780gagagggaaa gtatccaaag agaggccaaa aagtacaacc tcacatcaac caataggccg 840gaggaggaag ctagaggaat agtgattaga gacccaattg ggacctaatt gggacccaaa 900tttctcaagt ggagggagaa cttttgacga tttccaccgg tatctcctcg tgggtattca 960gggagctgct cagaaaccta taaacttgtc taaggcgact gaagtcgtcc aggggcatga 1020tgagtcacca ggagtgtttt tagagcacct ccaggaggct tatcagattt acaccccttt 1080tgacctggca gcccccgaaa atagccatgc tcttaatttg gcatttgtgg ctcaggcagc 1140cccagatagt aaaaggaaac tccaaaaact agagggattt tgctggaatg aataccagtc 1200agcttttaga gatagcctaa aaggtttttg acagtcaaga ggttgaaaaa caaaaacaag 1260cagctcaggc agctgaaaaa agccactgat aaagcatcct ggagtatcag agtttactgt 1320tagatcagcc tcatttgact tcccctccca catggtgttt aaatccagct acactacttc 1380ctgactcaaa ctccactatt cctgttcatg actgtcagga actgttggaa actactgaaa 1440ctggccgacc tgatcttcaa aatgtgcccc taggaaaggt ggatgccacc atgttcacag 1500acagtagcag cttcctcgag aagggactac gaaaggccgg tgcagctgtt accatggaga 1560cagatgtgtt gtgggctcag gctttaccag caaacacctc agcacaaaag gctgaattga 1620tcgccctcac tcaggctctc cgatggggta aggatattaa cgttaacact gacagcaggt 1680acgcctttgc tactgtgcat gtacgtggag ccatctacca ggagcgtggg ctactcacct 1740cagcaggtgg ctgtaatcca ctgtaaagga catcaaaagg aaaacacggc tgttgcccgt 1800ggtaaccaga aagctgattc agcagctcaa gatgcagtgt gactttcagt cacgcctcta 1860aacttgctgc ccacagtctc ctttccacag ccagatctgc ctgacaatcc cgcatactca 1920acagaagaag aaaactggcc tcagaactca gagccaataa aaatcaggaa ggttggtgga 1980ttcttcctga ctctagaatc ttcatacccc gaactcttgg gaaaacttta atcagtcacc 2040tacagtctac cacccattta ggaggagcaa agctacctca gctcctccgg agccgtttta 2100agatccccca tcttcaaagc ctaacagatc aagcagctct ccggtgcaca acctgcgccc 2160aggtaaatgc caaaaaaggt cctaaaccca gcccaggcca ccgtctccaa gaaaactcac 2220caggagaaaa gtgggaaatt gactttacag aagtaaaacc acaccgggct gggtacaaat 2280accttctagt actggtagac accttctctg gatggactga agcatttgct accaaaaacg 2340aaactgtcaa tatggtagtt aagtttttac tcaatgaaat catccctcga catgggctgc 2400ctgtttgcca tagggtctga taatggaccg gccttcgcct tgtctatagt ttagtcagtc 2460agtaaggcgt taaacattca atggaagctc cattgtgcct atcgacccca gagctctggg 2520caagtagaac gcatgaactg caccctaaaa aacactctta caaaattaat cttagaaacc 2580ggtgtaaatt gtgtaagtct ccttccttta gccctactta gagtaaggtg caccccttac 2640tgggctgggt tcttaccttt tgaaatcatg tatgggaggg tgctgcctat cttgcctaag 2700ctaagagatg cccaattggc aaaaatatca caaactaatt tattacagta cctacagtct 2760ccccaacagg tacaagatat catcctgcca cttgttcgag gaacccatcc caatccaatt 2820cctgaacaga cagggccctg ccattcattc ccgccaggtg acctgttgtt tgttaaaaag 2880ttccagagag aaggactccc tcctgcttgg aagagacctc acaccgtcat cacgatgcca 2940acggctctga aggtggatgg cattcctgcg tggattcatc actcccgcat caaaaaggcc 3000aacagagccc aactagaaac atgggtcccc agggctgggt caggcccctt aaaactgcac 3060ctaagttggg tgaagccatt agattaattc tttttcttaa ttttgtaaaa caatgcatag 3120cttctgtcaa acttatgtat cttaagactc aatataaccc ccttgttata actgaggaat 3180caatgatttg attcccccaa aaacacaagt ggggaatgta gtgtccaacc tggtttttac 3240taaccctgtt tttagactct ccctttcctt taatcactca gcttgtttcc acctgaattg 3300actctccctt agctaagagc gccagatgga ctccatcttg gctctttcac tggcagccgc 3360ttcctcaagg acttaacttg tgcaagctga ctcccagcac atccaagaat gcaattaact 3420gataagatac tgtggcaagc tatatccgca gttcccagga attcgtccaa ttgatcacag 3480cccctctacc cttcagcaac caccaccctg atcagtcagc agccatcagc accgaggcaa 3540ggccctccac cagcaaaaag attctgactc actgaagact tggatgatca ttagtatttt 3600tagcagtaaa gttttttttt ctttttcttt ctttttttct cgtgcc 3646 228 419 DNA Homo sapien misc_feature (1)...(419) n = A,T,C or G 228taagagggta caagatctaa gcacagccgt caatgcagaa cacagaacgt agcctggtaa 60gtgtgttaag agtgggaatt tttggagtac agagtaaggc acctaaccct agctggggtt 120tggtgacggt cccagatggc ttacagaaga aagtgtcctg agatgagttt ttaagaatga 180ataaggatag acacaagtga ggactgactt ggcagtggtg aatggtgggt ggcaaaaaac 240ttcgcatgta tggaaactgc acgtacagga atgaagaatg agactgtgtg gtgtttaatg 300agctgcaaat actaatttta tcctgaaagt tttgaagagt taactaaaaa gtatttttta 360gtaaggaaat aaccctacat ttcagggtta ttgtttgttt anatattgaa ggtgcccaa 419 229 148 DNA Homo sapien 229aagagggtac ctgtatgtag ccatggtggc aatgagagac tgattactac ctgctggaga 60ttgtttaagt gagttaatat attaaggata aagggagcca ggttttttga ctgttggaga 120aggaaattac agatattgaa ggtcccaa 148 230 257 DNA Homo sapien 230taagagggta cmaaaaaaaa aaaatagaac gaatgagtaa gacctactat ttgatagtac 60aacagggtga ctatagtcaa tgataactta attatacatt taacatagag tgtaattgga 120ttgtttgtaa ctcgaaggat aaatgcttga gaggatggat accccattct ccatgatgta 180cttatttcac attacatgcc tgtatcaaag catctcatat accctataaa tatgtacacc 240tactatgtac cctctta 257 231 260 DNA Homo sapien 231taagagggta cgggtatttg ctgatgggat ttttttttct ttctttttct ttggaaaaca 60aaatgaaagc cagaacaaaa ttattgaaca aaagacaggg actaaatctg gagaaatgaa 120gtcccctcac ctgactgcca tttcattcta tctgaccttc cagtctaggt taggagaata 180gggggtggag gggattaatc tgatacaggt atatttaaag caactctgca tgtgtgccag 240aagtccatgg taccctctta 260 232 596 DNA Homo sapien misc_feature (1)...(596) n = A,T,C or G 232tgctcctctt gccttaccaa ccacaaatta gaaccataat gagatgtcac ctcatacctg 60gtgggattaa cattatttaa aaaatcagaa gtattgacaa ggatgtgaag aaattagaac 120atctgtgcac tgttggtggg aatgtaaaaa aggtgtggcc actatgggta acagcatgaa 180ggttcctcaa aaaaaatttt ttttaatcta ctctatgatc gatcttgagg ttgtttatgc 240aaaagaactg aaatcaggat tttgaggaaa tattcacatt cccacatcca tttctgcttt 300attcataata ctcaagagat ggaaacaacc taaatgtcca tcccgggatg aatggataaa 360cacagtgtgg tatatgcata caatggaata ttatttagtc tttaaaaaga aaaattctat 420catatactac aacttanatn aaccttgagg acacaatgct nagtgaaata agccacggaa 480ggacgaatac tgcattattc ccttatatga agtatctaaa gtggtcaaac tcttanagca 540naaagtaaaa atgggtggtt gccanacagt tggttaggcn agaaganaan cctant 596 233 96 DNA Homo sapien 233tcttctgaag acctttcgcg actcttaagc tcgtggttgg taaggcaaga ggagcgttgg 60taaggcaaga ggagcgttgg taaggcaaga ggagca 96 234 313 DNA Homo sapien 234tgtaagtcga gcagtgtgat gataaaactt gaatggatca atagttgctt cttatggatg 60agcaaagaaa gtagtttctt gtgatggaat ctgctcctgg caaaaatgct gtgaacgttg 120ttgaaaagac aacaaagagt ttagagtagt acataaattt agaatagtac ataaacttag 180aatagtacat aaacttagta cataaataat gcacgaagca ggggcagggc ttgagagaat 240tgacttcaat ttggaaagag tatctactgt aggttagatg ctctcaaaca gcatcacact 300gctcgactta caa 313 235 550 DNA Homo sapien 235aacgaggaca gatccttaaa aagaatgttg agtgaaaaaa gtagaaaata agataatctc 60caaagtccag tagcattatt taaacatttt taaaaaatac actgataaaa attttgtaca 120tttcccaaaa atacatatgg aagcacagca gcatgaatgc ctatgggrtt gaggataggg 180gttgggagta gggatgggga taaaggggga aaataaaacc agagaggagt cttacacatt 240tcatgaacca aggagtataa ttatttcaac tatttgtacc wgaagtccag aaagagtgga 300ggcagaaggg ggagaagagg gcgaagaaac gtttttggga gaggggtccc asaagagaga 360ttttcgcgat gtggcgctac atacgttttt ccaggatgcc ttaagctctg caccctattt 420ttctcatcac taatattaga ttaaaccctt tgaagacagc gtctgtggtt tctctacttc 480agctttccct ccgtgtcttg cacacagtag ctgttttaca agggttgaac tgactgaagt 540gagattattc 550 236 325 DNA Homo sapien 236tagactgact catgtcccct accagagtag ctagaattaa tagcacaagc ctctacaccc 60aggaactcac tattgaatac ataaatggaa tttattcagc cttaaaaagt ttggaaggaa 120attctgacat atgctaaaac atggatgaac cttgaagact ttatgataag taaaagaagc 180cagtcataaa aggaaaaata ttgcatgatt ccacttatat gaggtaccta gagtagtcaa 240tttcatagaa acacaaaata gaatggtgtt tgccagggct tttgaggaaa agggaatgac 300aagttagggg acatgagtca gtcta 325 237 373 DNA Homo sapien misc_feature (1)...(373) n = A,T,C or G 237tagactgact catgtcccct atctactcaa catttccact tgaagtctga taggcatctc 60agacttatct tgtcccaaag caaactcttt atttcttttc atcctagtct ttatttcttg 120tgctgtctta cccatctcaa aagagtgcca aaatccacca agttgctgaa acagaaatct 180aagaaatatc cttgattctt ctttttccca tctacttcac ttctaattca ttagtaaata 240atctgtttca gaaaaccaaa cacctcatgt tctcactcat aagggggagt tgaacaatga 300gaacacacag acacagggag gggaacatca cacaccacgg cccgtcaggg agtangggac 360atgagtcagt cta 373 238 492 DNA Homo sapien misc_feature (1)...(492) n = A,T,C or G 238tagactgact catgtcccct ataatgctcc caggcatcag aaagcatctc aaactggagc 60tgacaccatg gcagaggttt caggtaagtc acaaaagggg tcctaaagaa tttgccctca 120atatcagagt gattagaaga agtggacaga gctacccaag ttaaacatat gcgagataaa 180aaaaatatgg cacttgtgaa cacacactac aggaggaaaa taaggaacat aatagcatat 240tgtgctatta tgatgatgaa gaacctctct anaagaaaac ataaccaaag aaacaaagaa 300aattcctgcn aatgtttaat gctatagaag aaattaacaa aaacatatat tcaatgaatt 360cagaaaagtt agcaggtcan aagaaaacaa atcaaagacc agaataatcc cattttagat 420tgtcgagtaa actanaacag aaagaatacc actggaaatt gaattcctac gtangggaca 480tgantcantc ta 492 239 482 DNA Homo sapien misc_feature (1)...(482) n = A,T,C or G 239tggaaagtat ttaatgatgg gcaacttgct gtttacttcc tacatatccc atcatcttct 60gtattttttt aaataacttt tttttggatt tttaaagtaa ccttattctg agaggtaaca 120tggattacat acttctaagc cattaggaga ctctatgtta aaccaaaagg aaatgttact 180agatcttcat ttgatcaata ggatgtgata atcatcatct ttctgctcta atggaaaagt 240actanaaaca tggaaccata atcttagatg aacaacgtta gaatttgcac taattctacg 300gaatttcagt aattcggcaa atgtcgggca gtgacacaac atttcatgac ggggacgcat 360ctaccaactt ctggcgataa gggccaccct tccctctgta cttacagtcc catttcatac 420acagtctttg attaaatatt cacatttttt ctctacctaa agaccttcaa gaccagtacg 480ta 482 240 519 DNA Homo sapien misc_feature (1)...(519) n = A,T,C or G 240tgtatcgacg tagtggtctc cccatgtgat agtctgaaat atagcctcat gggatgagag 60gctgtgcccc agcccgacac ccgtaaaggg tctgtgctga ggtggattag taaaagagga 120aagccttgca gttgagatag aggaagggca ctgtctcctg cctgcccctg ggaactgaat 180gtctcggtat aaaacccgat tgtacatttg ttcaattctg agataggaga aaaaccaccc 240tatggcggga ggcgagacat gttggcagca atgctgcctt gttatgcttt actccacaga 300tgtttgggcg gagggaaaca taaatctggc ctacgtgcac atccaggcat agtacctccc 360tttgaactta attatgacac agattccttt gctcacatgt ttttttgctg accttctcct 420tattatcacc ctgctctcct accgcattcc ttgtgctgag ataatgaaaa taatatcaat 480aaaaacttga nggaactcgg agaccactac gtcgataca 519 241 771 DNA Homo sapien misc_feature (1)...(771) n = A,T,C or G 241tgtatcgacg tagtggtctc cactcccgcc ttgacggggc tgctatctgc cttccaggcc 60actgtcacgg ctcccgggta gaagtcactt atgagacaca ccagtgtggc cttgttggct 120tgaagctcct cagaggaggg tgggaacaga gtgaccgagg gggcagcctt gggctgacct 180aggacggtca gcttggtccc tccgccaaac acgagagtgc tgctgcttgt atatgagctg 240cagtaataat cagcctcgtc ctcagcctgg agcccagaga tggtcaggga ggccgtgttg 300ccanacttgg agccagagaa gcgattagaa acccctgagg gccgattacc gacctcataa 360atcatgaatt tgggggcttt gcctgggtgc tgttggtacc angagacatt attataacca 420ccaacgtcac tgctggttcc antgcaggga aaatggttga tcnaactgtc caagaaaacc 480actacgtcca taccaatcca ctaattgccn gccgcctgca ggttcaacca tattggggaa 540naactccccn ccgccgtttg ggattgncat naacctttga aattttttcc tattanttgt 600ccccctaaaa taaaccnttg ggcnttaatc cattgggtcc atancttntt tncccggttt 660ttaaaanttg tttatcccgc cncccnattt cccccccaac tttccaaaac ccgaaaccnt 720tnaaatttnt tnaaaccctg gggggttccc nnaattnnan ttnaanctnc c 771 242 167 DNA Homo sapien 242tgggcacctt caatatcggg ctcatcgata acatcacgct gctgatgctg ctgttgctgg 60tcctctctag gaacctctgg attttcaaat tctttgagga attcatccaa attatctgcc 120tctcctcctt tcctcctttt tctaaggtct tctggtacaa gcggtca 167 243 338 DNA Homo sapien 243ttgggcacct tcaatatcta ctgatctaaa tagtgtggtt tgaggcctct tgttcctggc 60taaaaatcct tggcaagagt caatctccac tttacaatag aggtaaaaat cttacaatgg 120atattcttga caaagctagc atagagacag caattttaca caaggtattt ttcacctgtt 180taataacagt ggttttccta cacccatagg gtgccaccaa gggaggagtg cacagttgca 240gaaacaaatt aagatactga agacaacact acttaccatt tcccgtatag ctaaccacca 300gttcaactgt acatgtatgt tcttatgggc aatcaaga 338 244 346 DNA Homo sapien 244tttttggctc ccatacagca cactctcatg ggaaatgtct gttctaaggt caacccataa 60tgcaaaaatc atcaatatac ttgaagatcc ccgtgtaagg tacaatgtat ttaatattat 120cactgataca attgatccaa taccagtttt agtctggcat tgaatcaaat cactgttttt 180gttgtataaa aagagaaata tttagcttat atttaagtac catattgtaa gaaaaaagat 240gcttatcttt acatgctaaa atcatgatct gtacattggt gcagtgaata ttactgtaaa 300agggaagaag gaatgaagac gagctaagga tattgaaggt gcccaa 346 245 521 DNA Homo sapien misc_feature (1)...(521) n = A,T,C or G 245accaatccca cacggatact gagggacaag tatatcatcc catttcatcc ctacagcagc 60aacttcatga ggcaggagtt attagtccca ttttacagaa gaggaaactg agacttaggg 120agatcaagta atttgcccag gtcgcacaat tagtgataga gccagggctt gaagcgacgt 180ctgtcttaag ccaatgaccc ctgcagatta ttagagcaac tgttctccac aacagtgtaa 240gcctcttgct anaagctcag gtccacaagg gcagagattt ttgtctgttt tgctcattgc 300tccttcccca ttgcttagag cagggtctgc cacgaancag gttctcaatg catagttatt 360aaatgtatat aagagcaaac atatgttaca gagaactttc tgtatgcttg tcacttacat 420gaatcacctg tganatgggt atgcttgttc cccantgttg cagatnaaga tattgaangt 480gcccaaatca ctanttgcgg gcgcctgcan gtccancata t 521 246 482 DNA Homo sapien misc_feature (1)...(482) n = A,T,C or G 246tggaaccaat ccaaataccc atcaatgata gactggataa agaaaatttg gcacatgttc 60accatgaaat actatgcagc cataaaaaag gatgagttca tatcctttgc agggacatgg 120atgaagctgg agaccatcat tctcagcaaa ctaacaaggg aacagaaaac caaacactgc 180atgttctcac tcttaagtgg gagctgaaca atgagaacac atggacacag ggaggggaac 240atcacacagt ggggcctgct ggtgggtagg ggtctagggg agggatagca ttaggagaaa 300tacctaatgt agatgacggg ttgatgggtg cagcaaacca ccatgacacg tgtataccta 360tgtaacaaac ctgcatgttc tgcacatgta ccccagaact taaagtgtta ataaaaaaat 420taagaaaaaa gttaagtatg tcatagatac ataaaatatt gtanatattg aaggtgccca 480aa 482 247 474 DNA Homo sapien misc_feature (1)...(474) n = A,T,C or G 247ttcgatacag gcacagagta agcagaaaaa tggctgtggt ttaaccaagt gagtacagtt 60aagtgagaga ggggcagaga agacaagggc atatgcaggg ggtgattata acaggtggtt 120gtgctgggaa gtgagggtac tcggggatga ggaacagtga aaaagtggca aaaagtggta 180agatcagtga attgtacttc tccagaattt gatttctggn ggagtcaaat aactatccag 240tttggggtat catanggcaa cagttgaggt ataggaggta gaagtcncag tgggataatt 300gaggttatga anggtttggt actgactggt actgacaang tctgggttat gaccatggga 360atgaatgact gtanaagcgt anaggatgaa actattccac ganaaagggg tccnaaaact 420aaaaannnaa gnnnnngggg aatattattt atgtggatat tgaangtgcc caaa 474 248 355 DNA Homo sapien misc_feature (1)...(355) n = A,T,C or G 248ttcgatacag gcaaacatga actgcaggag ggtggtgacg atcatgatgt tgccgatggt 60ccggatggnc acgaagacgc actggancac gtgcttacgt ccttttgctc tgttgatggc 120cctgagggga cgcaggaccc ttatgaccct cagaatcttc acaacgggag atggcactgg 180attgantccc antgacacca gagacacccc aaccaccagn atatcantat attgatgtag 240ttcctgtaga nggccccctt gtggaggaaa gctccatnag ttggtcatct tcaacaggat 300ctcaacagtt tccgatggct gtgatgggca tagtcatant taaccntgtn tcgaa 355 249 434 DNA Homo sapien 249ttggattggt cctccaggag aacaagggga aaaaggtgac cgagggctcc ctggaactca 60aggatctcca ggagcaaaag gggatggggg aattcctggt cctgctggtc ccttaggtcc 120acctggtcct ccaggcttac caggtcctca aggcccaaag ggtaacaaag gctctactgg 180acccgctggc cagaaaggtg acagtggtct tccagggcct cctgggcctc caggtccacc 240tggtgaagtc attcagcctt taccaatctt gtcctccaaa aaaacgagaa gacatactga 300aggcatgcaa gcagatgcag atgataatat tcttgattac tcggatggaa tggaagaaat 360atttggttcc ctcaattccc tgaaacaaga catcgagcat atgaaatttc caatgggtac 420tcagaccaat ccaa 434 250 430 DNA Homo sapien misc_feature (1)...(430) n = A,T,C or G 250tggattggtc acatggcaga gacaggattc caaggcagtg agaggaggat acaatgcttc 60tcactagtta ttattattta ttttattttt gagatgaagt ctcgctttgt ctcccaggct 120ggagagcggt ggtgcgatct tggctctctg caacccccgc ctcaagcaat tctcctgtct 180tagcctcgcg ggtagatgga attacaggcg cccaccgcca tgcccaacta atttttttgt 240gtcttcagta gagacagggt ttcgccatgt tgggcaggct ggtcttgaac tcctgacctc 300nagtgatctg ccctcctcgg cctcacaaag tgctggaatt acaggcatgg gctgctgcac 360ccagtcaact tctcactagt tatggcctta tcattttcac cacattctat tggcccaaaa 420aaaaaaaaan 430 251 329 DNA Homo sapien 251tggtactcca ccatyatggg gtcaaccgcc atcctcgccc tcctcctggc tgttctccaa 60ggagtctgtg ccgaggtgca gctgrtgcag tctggagcag aggtgaaaaa gtccggggag 120tctctgaaga tctcctgtaa gggttctgga tacaccttta agatctactg gatcgcctgg 180gtgcgccagt tgcccgggaa aggcctggag tggatggggc tcatctttcc tgatgactct 240gataccagat acagcccgtc cttccaaggc caggtcacca tctcagtcga taagtccatc 300agcaccgcct atctgcagtg gagtaccaa 329 252 536 DNA Homo sapien 252tggtactcca ctcagcccaa ccttaattaa gaattaagag ggaacctatt actattctcc 60caggctcctc tgctctaacc aggcttctgg gacagtatta gaaaaggatg tctcaacaag 120tatgtagatc ctgtactggc ctaagaagtt aaactgagaa tagcataaat cagaccaaac 180ttaatggtcg ttgagacttg tgtcctggag cagctgggat aggaaaactt ttgggcagca 240agaggaagaa ctgcctggaa gggggcatca tgttaaaaat tacaagggga acccacacca 300ggcccccttc ccagctctca gcctagagta ttagcatttc tcagctagag actcacaact 360tccttgctta gaatgtgcca ccggggggag tccctgtggg tgatgaggct ctcaagagtg 420agagtggcat cctatcttct gtgtgcccac aggagcctgg cccgagactt agcaggtgaa 480gtttctggtc caggctttgc ccttgactca ctatgtgacc tctggtggag taccaa 536 253 507 DNA Homo sapien misc_feature (1)...(507) n = A,T,C or G 253ntgttgcgat cccagtaact cgggaagctg aggcgggagg atcacctgag ctcaggaggt 60tgaggccgca gtgagccggg accacgccac tacactccag cctggggcat agagtgagac 120cctccaagac agaaaagaaa agaaaggaag ggaaagggaa agggaaaagg aaaaggaaaa 180ggaaaaggaa aaggaaaaga caagacaaaa caagacttga atttggatct cctgacttca 240attttatgtt ctttctacac cacaattcct ctgcttacta agatgataat ttagaaaccc 300ctcgttccat tctttacagc aagctggaag tttggtcaag taattacaat aatagtaaca 360aatttgaata ttatatgcca ggtgtttttc attcctgctc tcacttaatt ctcaccactc 420tgatataaat acaattgctg ccgggtgtgg tggctcatgc ctgtaatccc ggcactttgg 480gagaccgagg tgggcggats gcaacaa 507 254 222 DNA Homo sapien misc_feature (1)...(222) n = A,T,C or G 254ttggattggt cactgtgagg aagccaaatc ggatccgaga gtctttttct aaaggccagt 60actggccaca ctttctcctg ccgccttcct caaagctgaa gacacacaga gcaaggcgct 120tctgttttac tccccaatgg taactccaaa ccatagatgg ttagctnccc tgctcatctt 180tccacatccc tgctattcag tatagtccgt ggaccaatcc aa 222 255 463 DNA Homo sapien 255tgttgcgatc cataaatgct gaaatggaaa taaacaacat gatgagggag gattaagttg 60gggagggagc acattaaggt ggccatgaag tttgttggaa gaagtgactt ttgaacaagg 120ccttggtgtt aagagctgat gagagtgtcc cagacagagg ggccactggt acaatagacg 180agatgggaga gggcttggaa ggtgtgcgaa ataggaagga gtttgttctg gtatgagtct 240agtgaacaca gaggcgagag gccctggtgg gtgcagctgg agagttatgc agaataacat 300taggccctgt gggggactgt agactgtcag caataatcca cagtttggat tttattctaa 360gagtgatggg aagccgtgga aagggggtta agcaaggagt gaaattatca gatttacagt 420gataaaaata aattggtctg gctactgggg aaaaaaaaaa aaa 463 256 262 DNA Homo sapien 256ttggattggt caacctgctc aactctacyt ttcctccttc ttcctaaaaa attaatgaat 60ccaatacatt aatgccaaaa cccttgggtt ttatcaatat ttctgttaaa aagtattatc 120cagaactgga cataatacta cataataata cataacaacc ccttcatctg gatgcaaaca 180tctattaata tagcttaaga tcactttcac tttacagaag caacatcctg ttgatgttat 240tttgatgttt ggaccaatcc aa 262 257 461 DNA Homo sapien misc_feature (1)...(461) n = A,T,C or G 257gnggnnnnnn nnncaattcg actcngttcc cntggtancc ggtcgacatg gccgcgggat 60taccgcttgt nnctgggggt gtatggggga ctatgaccgc ttgtagctgg gggtgtatgg 120gggactatga ccgcttgtag mtggkggtgt atgggggact atgaccgctt gtcgggtggt 180cggataaacc gacgcaaggg acgtgatcga agctgcgttc ccgctctttc gcatcggtag 240ggatcatgga cagcaatatc cgcattcgyc tgaaggcgtt cgaccatcgc gtgctcgatc 300aggcgaccgg cgacatcgcc gacaccgcac gccgtaccgg cgcgctcatc cgcggtccga 360tcccgcttcc cacgcgcatc gagaagttca cggtcaaccg tggcccgcac gtcgacaaga 420agtcgcgcga gcagttcgag gtgcgtacct acaagcggtc a 461 258 332 DNA Homo sapien misc_feature (1)...(332) n = A,T,C or G 258tgaccgcttg tagctggggg tgtatggggg actacgaccg cttgtagctg ggggtgtatg 60ggggactatg accgcttgta gctgggggtg tatgggggac tatgaccgct tgtagctggg 120ggtgtatggg ggactaggac cgcttgtagc tgggggtgta tgggggacta tgaccgcttg 180tagctggggg tgtatggggg actacgaccg cttgtagctg ggggtgtatg ggggactatg 240accgcttgta nctgggggtg tatgggggac tatgaccgct tgtgctgcct gggggatggg 300aggagagttg tggttgggga aaaaaaaaaa aa 332 259 291 DNA Homo sapien misc_feature (1)...(291) n = A,T,C or G 259taccgcttgt gaccgcttgt gaccgcttgt gaccgcttgt gaccgcttgt gaccgcttgt 60gaccgcttgt gaccgcttgt gaccgcttgt gaccgcttgt gaccgcttgt gaccgcttgt 120gaccgcttgt gaccgcttgt nacngggggt gtctggggga ctatgannga ntgtnactgg 180gggtgtctgg gggnctatga nngantgtna cngggggtgt ctgggggact atganngact 240gtgcnncctg ggggatcnga ggagantngn ggntagngat ggttngggan a 291 260 238 DNA Homo sapien 260taagagggta ctggttaaaa tacaggaaat ctggggtaat gaggcagaga accaggatac 60tttgaggtca gggatgaaaa ctagaatttt tttctttttt tttgcctgag aaacttgctg 120ctctgaagag gcccatgtat taattgcttt gatcttcctt ttcttacagc cctttcaagg 180gcagagccct ccttatcctg aaggaatctt atccttagct atagtatgta ccctctta 238 261 746 DNA Homo sapien misc_feature (1)...(746) n = A,T,C or G 261ttgggcacct tcaatatcaa tagctaacat ttattgagtg tttatcgtat cataaaacac 60tgttctaagc ctttaaacgt actaattcat ttaatgctca taatcacttt agaaggtggg 120tactagtatt agtctcattt acagatgcaa catgcaggca cagagaggtt aattaacttg 180cccaaggtaa cacagctaag aaatagaaaa aatattgaat ctggaaagtt gggcttctgg 240gtaacccaca gagtcttcaa tgagcctggg gcctcactca gtttgctttt acaaagcgaa 300tgagtaacat cacttaattc agtgagtagg ccaaatggag gtcagctacg agtttctgct 360gttcttgcag tggactgaca gatgtttaca acgtctggcc atcagtwaat ggactgatta 420tcattgggaw gtgggtgggc tgaatgttgg ccagtgaagt ttattcawgc catattttta 480tgtttaggat gacttttggc tggtcctagg gcaagctctg tctgscacgg aacacagaat 540wacacaggga ccccctcaat ttctggtgtg gctagaacca tgaaccactg gttgggggaa 600caagcggtca aaacctaagt gcggccggct ggcagggtcc acccatatgg ggaaaactcc 660cnacgcgttt ggaatgcctn agctngaatt attctaanag ttgtccncnt aaaattagcc 720tgggcgttaa tcangggtcn naagcc 746 262 588 DNA Homo sapien misc_feature (1)...(588) n = A,T,C or G 262tgaccgcttg tcatctcaca tggggtcctg cacgcttttg cctttgtagg aaacctgaca 60tttgtctgtt tcttctttct cttttccttc ccatatcctc ctaatttacg tttgacttgt 120ttgctgagga ggcaggagct agagactgct gtgagctcat aggggtggga agtttatcct 180tcaagtcccg cccactcatc actgcttctc accttcccct gaccaggctt acaagtgggt 240tcttgcctgc tttccctttg gacccaacaa gcccctgtaa tgagtgtgca tgactctgac 300agctgtggac tcagggtcct tggctacagc tgccatgtaa aatatctcat ccagttctcg 360caaattgtta aaataaccac atttcttaga ttccagtacc caaatcatgt ctttacgaac 420tgctcctcac acccagaagt ggcacaataa ttcttgggga attattactt ttttttttct 480ctctnttnnc gnnngnnnng gnnngnccag gaattaccac nttggaagac ctggccngaa 540tttattatan aggggagccg attntttttc ctaacacaaa gcgggtca 588 263 730 DNA Homo sapien misc_feature (1)...(730) n = A,T,C or G 263tttttttttt tttggcctga gcaactgaaa ttatgaaatt tccatatact caaaagagta 60agactgcaaa aagattaaat gtaaaagttg tcttgtatac agtaatgttt aagataccta 120ttanatttat aaatggaaaa ttagggcatt tggatataca agttgaaaat tcaggagtga 180ggttgggctg gctgggtata tactgaaaac tgtcagtaca cagatgacat ctaaaaccac 240aaatctggtt ttattttagc agtgatatgt gtcactccca caaaagcctt cccaattggc 300ctcagcatac acaacaagtc acctccccac agccctctac acataaacaa attccttagt 360ttagttcagg aggaaatgcg cccttttcct tccgctctag gtgaccgcaa ggcccagttc 420tcgtcaccaa gatgttaagg gaagtctgcc aaagaggcat ctgaaaggaa ataaggggaa 480tgggagtgac cacaaaggaa agccaaggan aaactttgga gaccgtttct aganccctgg 540catttcacaa caaaactcng gaacaaacct tgtctcatca atcatttaag cccttcgttt 600ggannagact ttctgaactg ggcgctgaac ataancctca ttgaatgtct tcacagtctc 660ccagctgaag gcacaccttg ggccagaagg ggaatcttcc aggtcctcaa nacagggctc 720gccctttgnc 730 264 715 DNA Homo sapien misc_feature (1)...(715) n = A,T,C or G 264tttttttttt tttggccagt atgatagtct ctaccactat attgaagctc ttaggtcatt 60tacacttaat gtggttatag atgctgttga gcttacttct accaccttgc tatttctccc 120gtctcttttt tgttcctttt ctcttctttt cctcccttat tttataattg aattttttag 180gattctattt tatatagatt tatcagctat aacactttgt attcttttgt tttgtggttc 240ttctgtcatt tcaatgtgca tcttaaactc atcacaatct attttcaaat aatatcatat 300aaccttacat ataatgtaag aatctaccac catatatttc catttctccc ttccatccta 360tgtntgtcat attttttcct ttatatatgt tttaaagaca taatagtata tgggaggttt 420ttgcttaaaa tgtgatcaat attccttcaa ngaaacgtaa aaattcaaaa taaatntctg 480tttattctca aatnnaccta atatttccta ccatntctna tacntttcaa gaatctgaag 540gcattggttt tttccggctt aagaacctcc tctaaagcac tctaagcaga attaagtctt 600ctgggagagg aattctccca agcttgggcc ttnanntgta ctccntnang gttaaanttt 660ggccgggaaa tagaaattcc aagttaacag gntanttttt ntttttnttn tcncc 715 265 152 DNA Homo sapien 265tttttttttt tttcccaaca caaagcacca ttatctttcc tcacaatttt caacatagtt 60tgattcccat gaagaggtta tgatttctaa agaaaacatg gctactatac tatcaatcag 120ggttaaatct tttttttttg agacggagtt ta 152 266 193 DNA Homo sapien misc_feature (1)...(193) n = A,T,C or G 266taaactccgt ccccttctta atcaatatgg aggctaccca ctccacatta ccttcttttc 60aagggactgt ttccgtaact gttgtgggta ttcacgacca ggcttctaaa cctcttaaaa 120ctccccaatt ctggtgccaa cttggacaac atgctttttt tttttttttt tttttttttn 180gagacggagt tta 193 267 460 DNA Homo sapien 267tgttgcgatc ccttaagcat gggtgctatt aaaaaaatgg tggagaagaa aatacctgga 60atttacgtct tatctttaga gattgggaag accctgatgg aggacgtgga gaacagcttc 120ttcttgaatg tcaattccca agtaacaaca gtgtgtcagg cacttgctaa ggatcctaaa 180ttgcagcaag gctacaatgc tatgggattc tcccagggag gccaatttct gagggcagtg 240gctcagagat gcccttcacc tcccatgatc aatctgatct cggttggggg acaacatcaa 300ggtgtttttg gactccctcg atgcccagga gagagctctc acatctgtga cttcatccga 360aaaacactga atgctggggc gtactccaaa gttgttcagg aacgcctcgt gcaagccgaa 420tactggcatg acccataaaa ggaggatgtg gatcgcaaca 460 268 533 DNA Homo sapien misc_feature (1)...(533) n = A,T,C or G 268tgttgcgatc cgttgataga atagcgacgt ggtaatgagt gcatggcacg cctccgactt 60accttcgccc gtggggaccc cgagtacgtc tacggcgtcg tcacttagag taccctctgg 120acgcccgggc gcgttcgatt taccggaagc gcgagctgca gtgggcttgc gcccccggcc 180aaattctttg gggggtttaa ggccgcgggg aatttgaggt atctctatca gtatgtagcc 240aagttggaac agtcgccatt cccgaaatcg ctttctttga atccgcaccg cctccagcat 300tgcctcattc atcaacctga aggcacgcat aagtgacggt tgtgtcttca gcagctccac 360tccataacta gcgcgctcga cctcgtcttc gtacgcgcca ggtccgtgcg tgcgaattcc 420caactccggt gagttgcgca tttcaagttn cgaaactgtt cgcctccacn atttggcatg 480ttcacgcatg acacggaata aactcgtcca gtaccgggaa tgggatcgca aca 533 269 50 DNA Homo sapien 269tttttttttt ttcgcctgaa ttagctacag atcctcctca caagcggtca 50 270 519 DNA Homo sapien 270tgttgcgatc caaataaccc accagcttct tgcacacttc gcagaagcca ccgtcctttg 60gctgagtcac gtgaacggtc agtgcaagca gccgcgtgcc agagcagagg tgcagcatgc 120tgcacaccag ctcagggctg acctcctcca gcaggatgga caggatggag ctgccgtacg 180tgtccaccac ctcctggcac tcttccgaca gggacttcgg cagcttcgag cacattttgt 240caaaagcgtc gagtatttct ttctcagtct tgttgttgtc aatcagcttg gtcacctcct 300tcaccaggaa ttcacacacc tcacagtaaa catcagactt tgctgggacc tcgtgcttct 360taatgggctc caccagttcc agggcaggga tgacattctt ggaggccact ttggcgggga 420ccagagtctg catgggcatc tctttcacct catcacagaa cccaaccagc gcacagatct 480ccttgggttg catgtgcatc atcatctggg atcgcaaca 519 271 457 DNA Homo sapien 271tttttttttt ttcgggcggc gaccggacgt gcactcctcc agtagcggct gcacgtcgtg 60ccaatggccc gctatgagga ggtgagcgtg tccggcttcg aggagttcca ccgggccgtg 120gaacagcaca atggcaagac cattttcgcc tactttacgg gttctaagga cgccgggggg 180aaaagctggt gccccgactg cgtgcaggct gaaccagtcg tacgagaggg gctgaagcac 240attagtgaag gatgtgtgtt catctactgc caagtaggag aagagcctta ttggaaagat 300ccaaataatg acttcagaaa aaacttgaaa gtaacagcag tgcctacact acttaagtat 360ggaacacctc aaaaactggt agaatctgag tgtcttcagg ccaacctggt ggaaatgttg 420ttctctgaag attaagattt taggatggca atcaaga 457 272 102 DNA Homo sapien 272tttttttttt ttgggcaaca acctgaatac cttttcaagg ctctggcttg ggctcaagcc 60cgcaggggaa atgcaactgg ccaggtcaca gggcaatcaa ga 102 273 455 DNA Homo sapien misc_feature (1)...(455) n = A,T,C or G 273tttttttttt ttggcaatca acaggtttaa gtcttcggcc gaagttaatc tcgtgttttt 60ggcaatcaac aggtttaagt cttcggccga agttaatctc gtgtttttgg caatcaacag 120gtttaagtct tcggccgaag ttaatctcgt gtttttggca atcaacaggt ttaagtcttc 180ggccgaagtt aatctcgtgt ttttggcaat caacaggttt aagtcttcgg ccgaagttaa 240tctcgtgttt ttggcaatca acaggtttaa gtcttcggcc gaagttaatc tcgtgttttt 300ggcaatcaag aggtttaagt cttcggccga agttaatctc gtgtttttgg caatcaacag 360gtttaagtct tcggccgaan ttaatctcgt gtttttggca atcaacaggt ttaantcttc 420ggccgaagtt aatctcgtgt ttttggcaat caana 455 274 461 DNA Homo sapien 274tttttttttt ttggccaata cccttgatga acatcaatgt gaaaatcctc ggtaaaatac 60tggcaaacca aatccagcag cacatcaaaa agcttatcca ccatgatcaa gtgggcttca 120tccctgggat gcaaggctgg ttcaacataa gaaaatcaat aaatgtaatc catcacataa 180acagaaccaa agacaaaaac cacatgatta tctcaataga tgcagaaaag gccttggaca 240aattcaacag cccttcatgc taaacactct taataaacta gatattgatg gaatgtatct 300caaaataata agagctattt atgacaaacc cacagccaat atcatactga atgggcaaag 360actggaagca ttccctttga aaactggcac aagacaagga tgccctctct caccgctcct 420attcaacata gtattggaag ttctggccag ggcaatcaag a 461 275 729 DNA Homo sapien misc_feature (1)...(729) n = A,T,C or G 275tttttttttt ttggccaaca ccaagtcttc cacgtgggag gttttattat gttttacaac 60catgaaaaca taggaaggtg gctgttacag caaacatttc agatagacga atcggccaag 120ctccccaaac cccaccttca cagcctcttc cacacgtctc ccanagattg ttgtccttca 180cttgcaaatt canggatgtt ggaagtngac atttnnagtn gcnggaaccc catcagtgaa 240ncantaagca gaantacgat gactttgana nacanctgat gaagaacacn ctacnganaa 300ccctttctnt cgtgttanga tctcnngtcc ntcactaatg cggccccctg cnggtccacc 360atttgggaga actccccccn cgttggatcc ccccttgagt ntcccattct ngtcccccan 420accngncttg ngngncantn cnncctcnca ccntgtttcc ctgnngtnaa aatnngtttt 480nccgccnccc naattcccac ccnaatcaca gcgaanccng aaggccttcn naagtgttta 540angcccngng gtttcctcnt ntanttgcag cctaccctcc cncttnnnnt tncgngttgg 600tcgcgccctg gncncgcctn gttcctcttt nnggnnacaa cctngntcnn nggcncntcn 660nnnctnttcc tnnnactagc tngcctntcc ncnccgnggn ncanngcaca ttncncnnac 720tntgtnncc 729 276 339 DNA Homo sapien 276tgacctgaca tgtagtagat acttaataaa tatttgtgga atgaatggat gaagtggagt 60tacagagaaa aatagaaaag tacaaattgt tgtcagtgtt ttgaaggaaa attatgatct 120ttcccaaagt tctgacttca ttctaagaca gggttagtat ctccatacat aattttactt 180gcttttgaaa atcaaatgag ataatctatt tagattgata atttatttag actggctata 240aactattaag tgctagcaaa tatacatttt aatctcattt tccacctctt gtgatatagc 300tatgtaggtg ttgactttaa tggatgtcag gtcaatccc 339 277 664 DNA Homo sapien misc_feature (1)...(664) n = A,T,C or G 277tgacctgaca tccataacaa aatctttctc cattatattc ttctagggga atttcttgaa 60aagcatccaa aggaaacaaa tgatggtaag accgtgccaa gtggggagca gacaccaaag 120taagaccaca gattttacat tcaacaggta gctcacagta ctttgcccga cactgtgggc 180agaaatagcc tcctaatgta agccctggct cagtattgcc atccaaatgc gccatgctga 240aagagggttt tgcatcctgg tcagatnaag aagcaatggt gtgctgagga aatcccatac 300gaataagtga gcattcagaa cttgagctag caggaggagg actaagatga tgtgtgagca 360actctttgta atggctttca tctaaaataa catggtacgt gccaccagtt tcacgagcaa 420gtacagtgca aacgcgaact tctgcagaca atccaataac agatactcta attttagctg 480cctttagggt cttgattaaa tcataaatat tagatggatc gcaagttgta aggntgctaa 540aagatgatta gtacttctcg acttgtatgt ccaggcatgt tgttttaaan tctgccttag 600nccctgctta ggggaatttt taaagaagat ggctctccat gttcanggtc aatcacnaat 660tgcc 664 278 452 DNA Homo sapien misc_feature (1)...(452) n = A,T,C or G 278tgacctgaca ttgaggaaga gcacacacct ctgaaattcc ttaggttcag aagggcattt 60gacacagagt gggcctctga taattcatga aatgcattct gaagtcatcc agaatggagg 120ctgcaatctg ctgtgctttg ggggttgcct cactgtgctc ctggatatca cacaaaagct 180gcaatccttc ttcttcaact aacattttgc agtatttgct gggattttta ctgcagacat 240gatacatagc ccatagtgcc cagagctgaa cctctggttg agagaagttg ccaaggagcg 300ggaaaaatgt cttgaaagat ctataggtca ccaatgctgt catcttacaa cttgaacttg 360gccaattctg tatggttgca tgcagatctt ggagaagagt acgcctctgg aagtcacggg 420atatccaaan ctgtctgtca gatgtcaggt ca 452 279 274 DNA Homo sapien 279tttttttttt ttcggcaagg caaatttact tctgcaaaag ggtgctgctt gcacttttgg 60ccactgcgag agcacaccaa acaaagtagg gaaggggttt ttatccctaa cgcggttatt 120ccctggttct gtgtcgtgtc cccattggct ggagtcagac tgcacaatct acactgaccc 180aactggctac tgtttaaaat tgaatatgaa taattaggta ggaaggggga ggctgtttgt 240tacggtacaa gacgtgtttg ggcatgtcag gtca 274 280 272 DNA Homo sapien 280tacctgacat ggagaaataa cttgtagtat tttgcgtgca atggaatact atatgagggt 60gaaaatgaat gaactagcaa tgcgtgtatc aacatgaata aatccccaaa acataataat 120gttgaatgga aaaggtgagt ttcagaagga tatatatgcc ctctaaatcc atttatgtaa 180acctttaaaa aactacatta tttatggtca taagtccatc cagaaaatat ttaaaaacct 240acatgggatt gataactact gatgtcaggt ca 272 281 431 DNA Homo sapien misc_feature (1)...(431) n = A,T,C or G 281tttttttttt ttggccaata gcatgattta aacattggaa aaagtcaaat gagcaatgcg 60aatttttatg ttctcttgaa taatcaaaag agtaggcaac attggttcct cattcttgaa 120tagcattaat cagaaaatat tgcatagcct ctagcctcct tagagtaggt gtgctctctc 180aaatatatca tagtcccaca gtttatttca tgtatatttt ctgcctgaat cacatagaca 240tttgaatttg caacgcctga tgtaaatata taaattctta ccaatcagaa acatagcaag 300aaattcaggg acttggtcat yatcagggta tgacagcana tccctgtara aacactgata 360cacactcaca cacgtatgca acgtggagat gtcgcyttww kkktwywcwm rmrycrwcgn 420aatcacttan n 431 282 98 DNA Homo sapien 282attcgattcg atgcttgagc ccaggagttc aagactgcag tgagccactg cacttcaggc 60tggacaacag agcgagtccc tgtgccaaaa aaaaaaaa 98 283 764 DNA Homo sapien misc_feature (1)...(764) n = A,T,C or G 283tttttttttt ttcgcaagca cgtgcacttt attgaatgac actgtagaca ggtgtgtggg 60tataaactgc tgtatctagg ggcaggacca agggggcagg ggcaacagcc ccagcgtgca 120gggccascat tgcacagtgg astgcaaagg ttgcaggcta tgggcggcta ctavtaaccc 180cgtttttcct gtattatctg taacataata tggtagactg tcacagagcc gaatwccart 240hacasgatga atccaawggt caygaggatg cccasaatca gggcccasat sttcaggcac 300ttggcggtgg gggcatasgc ctgkgccccg gtcacgtcsc caaccwtcty cctgtcccta 360cmcttgawtc cncnccttnn nntnccntna tntgcccgcc cncctcctng ngtcaaccng 420natctgcact anctccctcn ccccttntgg antctcntcc ttcaantaan nttatccttn 480acncccccct cncctttccc ctnccncccn tnatcccngn nccnctatca ntcntnccct 540cnctntnctn cnnatcgttc cncctnntaa ctacnctttn nacnanncct cactnatncc 600ngnnanttct ttccttccct cccnacgcnn tgcgtgcgcc cgtctngcct nnnctncgna 660cccnnacttt atttaccttt ncaccctagc nctctacttn acccanccnc tcctacctcc 720nggnccaccc nnccctnatc nctnnctctn tcnnctcntt cccc 764 284 157 DNA Homo sapien 284caagtgtagg cacagtgatg aaagcctgga gcaaacacaa tctgtgggta attaacgttt 60atttctcccc ttccaggaac gtcttgcatg gatgatcaaa gatcagctcc tggtcaacat 120aaataagcta gtttaagata cgttccccta cacttga 157 285 150 DNA Homo sapien 285attcgattgt actcagacaa caatatgcta agtggaagaa gtcagtcaca aaagaccaca 60tactgtatga cttcatttac attaagtgtc cagaataggc aaatccgtag agacagaaag 120tagatgagca gctgcctagg tctgagtaca 150 286 219 DNA Homo sapien 286attcgatttt tttttttttg gccatgatga aattcttact ccctcagatt ttttgtctgg 60ataaatgcaa gtctcaccac cagatgtgaa attacagtaa actttgaagg aatctcctga 120gcaaccttgg ttaggatcaa tccaatattc accatctggg aagtcaggat ggctgagttg 180caggtcttta caagttcggg ctggattggt ctgagtaca 219 287 196 DNA Homo sapien 287attcgattct tgaggctacc aggagctagg agaagaggca tggaacaaat tttccctcat 60atccatactc agaaggaacc aaccctgctg acaccttaat ttcagcttct ggcctctaga 120actgtgagag agtacatttc tcttggttta agccaagaga atctgtcttt tggtacttta 180tatcatagcc tcaaga 196 288 199 DNA Homo sapien 288attcgatttc agtccagtcc cagaacccac attgtcaatt actactctgt araagattca 60tttgttgaaa ttcattgagt aaaacattta tgatccctta atatatgcca attaccatgc 120taggtactga agattcaagt gaccgagatg ctagcccttg ggttcaagtg atccctctcc 180cagagtgcac tggactgaa 199 289 182 DNA Homo sapien 289attcgattct tgaggctaca aacctgtaca gtatgttact ctactgaata ctgtaggcaa 60tagtaataca gaagcaagta tctgtatatg taaacattaa aaaggtacag tgaaacttca 120gtattataat cttagggacc accattatat atgtggtcca tcattggcca aaaaaaaaaa 180aa 182 290 1646 DNA Homo sapien 290ggcacgagga gaaatgtaat tccatatttt atttgaaact tattccatat tttaattgga 60tattgagtga ttgggttatc aaacacccac aaactttaat tttgttaaat ttatatggct 120ttgaaataga agtataagtt gctaccattt tttgataaca ttgaaagata gtattttacc 180atctttaatc atcttggaaa atacaagtcc tgtgaacaac cactctttca cctagcagca 240tgaggccaaa agtaaaggct ttaaattata acatatggga ttcttagtag tatgtttttt 300tcttgaaact cagtggctct atctaacctt actatctcct cactctttct ctaagactaa 360actctaggct cttaaaaatc tgcccacacc aatcttagaa gctctgaaaa gaatttgtct 420ttaaatatct tttaatagta acatgtattt tatggaccaa attgacattt tcgactattt 480tttccaaaaa agtcaggtga atttcagcac actgagttgg gaatttctta tcccagaaga 540ccaaccaatt tcatatttat ttaagattga ttccatactc cgttttcaag gagaatccct 600gcagtctcct taaaggtaga acaaatactt tctatttttt tttcaccatt gtgggattgg 660actttaagag gtgactctaa aaaaacagag aacaaatatg tctcagttgt attaagcacg 720gacccatatt atcatattca cttaaaaaaa tgatttcctg tgcacctttt ggcaacttct 780cttttcaatg tagggaaaaa cttagtcacc ctgaaaaccc acaaaataaa taaaacttgt 840agatgtgggc agaaggtttg ggggtggaca ttgtatgtgt ttaaattaaa ccctgtatca 900ctgagaagct gttgtatggg tcagagaaaa tgaatgctta gaagctgttc acatcttcaa 960gagcagaagc aaaccacatg tctcagctat attattattt attttttatg cataaagtga 1020atcatttctt ctgtattaat ttccaaaggg ttttaccctc tatttaaatg ctttgaaaaa 1080cagtgcattg acaatgggtt gatatttttc tttaaaagaa aaatataatt atgaaagcca 1140agataatctg aagcctgttt tattttaaaa ctttttatgt tctgtggttg atgttgtttg 1200tttgtttgtt tctattttgt tggtttttta ctttgttttt tgttttgttt tgttttgttt 1260kgcatactac atgcagttct ttaaccaatg tctgtttggc taatgtaatt aaagttgtta 1320atttatatga gtgcatttca actatgtcaa tggtttctta atatttattg tgtagaagta 1380ctggtaattt ttttatttac aatatgttta aagagataac agtttgatat gttttcatgt 1440gtttatagca gaagttattt atttctatgg cattccagcg gatattttgg tgtttgcgag 1500gcatgcagtc aatattttgt acagttagtg gacagtattc agcaacgcct gatagcttct 1560ttggccttat gttaaataaa aagacctgtt tgggatgtat tttttatttt taaaaaaaaa 1620aaaaaaaaaa aaaaaaaaaa aaaaaa 1646 291 1851 DNA Homo sapien 291tcatcaccat tgccagcagc ggcaccgtta gtcaggtttt ctgggaatcc cacatgagta 60cttccgtgtt cttcattctt cttcaatagc cataaatctt ctagctctgg ctggctgttt 120tcacttcctt taagcctttg tgactcttcc tctgatgtca gctttaagtc ttgttctgga 180ttgctgtttt cagaagagat ttttaacatc tgtttttctt tgtagtcaga aagtaactgg 240caaattacat gatgatgact agaaacagca tactctctgg ccgtctttcc agatcttgag 300aagatacatc aacattttgc tcaagtagag ggctgactat acttgctgat ccacaacata 360cagcaagtat gagagcagtt cttccatatc tatccagcgc atttaaattc gcttttttct 420tgattaaaaa tttcaccact tgctgttttt gctcatgtat accaagtagc agtggtgtga 480ggccatgctt gttttttgat tcgatatcag caccgtataa gagcagtgct ttggccatta 540atttatcttc attgtagaca gcatagtgta gagtggtatt tccatactca tctggaatat 600ttggatcagt gccatgttcc agcaacatta acgcacattc atcttcctgg cattgtacgg 660cctttgtcag agctgtcctc tttttgttgt caaggacatt aagttgacat cgtctgtcca 720gcacgagttt tactacttct gaattcccat tggcagaggc cagatgtaga gcagtcctct 780tttgcttgtc cctcttgttc acatccgtgt ccctgagcat gacgatgaga tcctttctgg 840ggactttacc ccaccaggca gctctgtgga gcttgtccag atcttctcca tggacgtggt 900acctgggatc catgaaggcg ctgtcatcgt agtctcccca agcgaccacg ttgctcttgc 960cgctcccctg cagcagggga agcagtggca gcaccacttg cacctcttgc tcccaagcgt 1020cttcacagag gagtcgttgt ggtctccaga agtgcccacg ttgctcttgc cgctccccct 1080gtccatccag ggaggaagaa atgcaggaaa tgaaagatgc atgcacgatg gtatactcct 1140cagccatcaa acttctggac agcaggtcac ttccagcaag gtggagaaag ctgtccaccc 1200acagaggatg agatccagaa accacaatat ccattcacaa acaaacactt ttcagccaga 1260cacaggtact gaaatcatgt catctgcggc aacatggtgg aacctaccca atcacacatc 1320aagagatgaa gacactgcag tatatctgca caacgtaata ctcttcatcc ataacaaaat 1380aatataattt tcctctggag ccatatggat gaactatgaa ggaagaactc cccgaagaag 1440ccagtcgcag agaagccaca ctgaagctct gtcctcagcc atcagcgcca cggacaggar 1500tgtgtttctt ccccagtgat gcagcctcaa gttatcccga agctgccgca gcacacggtg 1560gctcctgaga aacaccccag ctcttccggt ctaacacagg caagtcaata aatgtgataa 1620tcacataaac agaattaaaa gcaaagtcac ataagcatct caacagacac agaaaaggca 1680tttgacaaaa tccagcatcc ttgtatttat tgttgcagtt ctcagaggaa atgcttctaa 1740cttttcccca tttagtatta tgttggctgt gggcttgtca taggtggttt ttattacttt 1800aaggtatgtc ccttctatgc ctgttttgct gagggtttta attctcgtgc c 1851 292 1851 DNA Homo sapien 292tcatcaccat tgccagcagc ggcaccgtta gtcaggtttt ctgggaatcc cacatgagta 60cttccgtgtt cttcattctt cttcaatagc cataaatctt ctagctctgg ctggctgttt 120tcacttcctt taagcctttg tgactcttcc tctgatgtca gctttaagtc ttgttctgga 180ttgctgtttt cagaagagat ttttaacatc tgtttttctt tgtagtcaga aagtaactgg 240caaattacat gatgatgact agaaacagca tactctctgg ccgtctttcc agatcttgag 300aagatacatc aacattttgc tcaagtagag ggctgactat acttgctgat ccacaacata 360cagcaagtat gagagcagtt cttccatatc tatccagcgc atttaaattc gcttttttct 420tgattaaaaa tttcaccact tgctgttttt gctcatgtat accaagtagc agtggtgtga 480ggccatgctt gttttttgat tcgatatcag caccgtataa gagcagtgct ttggccatta 540atttatcttc attgtagaca gcatagtgta gagtggtatt tccatactca tctggaatat 600ttggatcagt gccatgttcc agcaacatta acgcacattc atcttcctgg cattgtacgg 660cctttgtcag agctgtcctc tttttgttgt caaggacatt aagttgacat cgtctgtcca 720gcacgagttt tactacttct gaattcccat tggcagaggc cagatgtaga gcagtcctct 780tttgcttgtc cctcttgttc acatccgtgt ccctgagcat gacgatgaga tcctttctgg 840ggactttacc ccaccaggca gctctgtgga gcttgtccag atcttctcca tggacgtggt 900acctgggatc catgaaggcg ctgtcatcgt agtctcccca agcgaccacg ttgctcttgc 960cgctcccctg cagcagggga agcagtggca gcaccacttg cacctcttgc tcccaagcgt 1020cttcacagag gagtcgttgt ggtctccaga agtgcccacg ttgctcttgc cgctccccct 1080gtccatccag ggaggaagaa atgcaggaaa tgaaagatgc atgcacgatg gtatactcct 1140cagccatcaa acttctggac agcaggtcac ttccagcaag gtggagaaag ctgtccaccc 1200acagaggatg agatccagaa accacaatat ccattcacaa acaaacactt ttcagccaga 1260cacaggtact gaaatcatgt catctgcggc aacatggtgg aacctaccca atcacacatc 1320aagagatgaa gacactgcag tatatctgca caacgtaata ctcttcatcc ataacaaaat 1380aatataattt tcctctggag ccatatggat gaactatgaa ggaagaactc cccgaagaag 1440ccagtcgcag agaagccaca ctgaagctct gtcctcagcc atcagcgcca cggacaggar 1500tgtgtttctt ccccagtgat gcagcctcaa gttatcccga agctgccgca gcacacggtg 1560gctcctgaga aacaccccag ctcttccggt ctaacacagg caagtcaata aatgtgataa 1620tcacataaac agaattaaaa gcaaagtcac ataagcatct caacagacac agaaaaggca 1680tttgacaaaa tccagcatcc ttgtatttat tgttgcagtt ctcagaggaa atgcttctaa 1740cttttcccca tttagtatta tgttggctgt gggcttgtca taggtggttt ttattacttt 1800aaggtatgtc ccttctatgc ctgttttgct gagggtttta attctcgtgc c 1851 293 668 DNA Homo sapien 293cttgagcttc caaataygga agactggccc ttacacasgt caatgttaaa atgaatgcat 60ttcagtattt tgaagataaa attrgtagat ctataccttg ttttttgatt cgatatcagc 120accrtataag agcagtgctt tggccattaa tttatctttc attrtagaca gcrtagtgya 180gagtggtatt tccatactca tctggaatat ttggatcagt gccatgttcc agcaacatta 240acgcacattc atcttcctgg cattgtacgg cctgtcagta ttagacccaa aaacaaatta 300catatcttag gaattcaaaa taacattcca cagctttcac caactagtta tatttaaagg 360agaaaactca tttttatgcc atgtattgaa atcaaaccca cctcatgctg atatagttgg 420ctactgcata cctttatcag agctgtcctc tttttgttgt caaggacatt aagttgacat 480cgtctgtcca gcaggagttt tactacttct gaattcccat tggcagaggc cagatgtaga 540gcagtcctat gagagtgaga agacttttta ggaaattgta gtgcactagc tacagccata 600gcaatgattc atgtaactgc aaacactgaa tagcctgcta ttactctgcc ttcaaaaaaa 660aaaaaaaa 668 294 1512 DNA Homo sapien 294gggtcgccca gggggsgcgt gggctttcct cgggtgggtg tgggttttcc ctgggtgggg 60tgggctgggc trgaatcccc tgctggggtt ggcaggtttt ggctgggatt gacttttytc 120ttcaaacaga ttggaaaccc ggagttacct gctagttggt gaaactggtt ggtagacgcg 180atctgttggc tactactggc ttctcctggc tgttaaaagc agatggtggt tgaggttgat 240tccatgccgg ctgcttcttc tgtgaagaag ccatttggtc tcaggagcaa gatgggcaag 300tggtgctgcc gttgcttccc ctgctgcagg gagagcggca agagcaacgt gggcacttct 360ggagaccacg acgactctgc tatgaagaca ctcaggagca agatgggcaa gtggtgccgc 420cactgcttcc cctgctgcag ggggagtggc aagagcaacg tgggcgcttc tggagaccac 480gacgaytctg ctatgaagac actcaggaac aagatgggca agtggtgctg ccactgcttc 540ccctgctgca gggggagcrg caagagcaag gtgggcgctt ggggagacta cgatgacagt 600gccttcatgg agcccaggta ccacgtccgt ggagaagatc tggacaagct ccacagagct 660gcctggtggg gtaaagtccc cagaaaggat ctcatcgtca tgctcaggga cactgacgtg 720aacaagaagg acaagcaaaa gaggactgct ctacatctgg cctctgccaa tgggaattca 780gaagtagtaa aactcstgct ggacagacga tgtcaactta atgtccttga caacaaaaag 840aggacagctc tgayaaaggc cgtacaatgc caggaagatg aatgtgcgtt aatgttgctg 900gaacatggca ctgatccaaa tattccagat gagtatggaa ataccactct rcactaygct 960rtctayaatg aagataaatt aatggccaaa gcactgctct tatayggtgc tgatatcgaa 1020tcaaaaaaca aggtatagat ctactaattt tatcttcaaa atactgaaat gcattcattt 1080taacattgac gtgtgtaagg gccagtcttc cgtatttgga agctcaagca taacttgaat 1140gaaaatattt tgaaatgacc taattatctm agactttatt ttaaatattg ttattttcaa 1200agaagcatta gagggtacag tttttttttt ttaaatgcac ttctggtaaa tacttttgtt 1260gaaaacactg aatttgtaaa aggtaatact tactattttt caatttttcc ctcctaggat 1320ttttttcccc taatgaatgt aagatggcaa aatttgccct gaaataggtt ttacatgaaa 1380actccaagaa aagttaaaca tgtttcagtg aatagagatc ctgctccttt ggcaagttcc 1440taaaaaacag taatagatac gaggtgatgc gcctgtcagt ggcaaggttt aagatatttc 1500tgatctcgtg cc 1512 295 1853 DNA Homo sapien 295gggtcgccca gggggsgcgt gggctttcct cgggtgggtg tgggttttcc ctgggtgggg 60tgggctgggc trgaatcccc tgctggggtt ggcaggtttt ggctgggatt gacttttytc 120ttcaaacaga ttggaaaccc ggagttacct gctagttggt gaaactggtt ggtagacgcg 180atctgttggc tactactggc ttctcctggc tgttaaaagc agatggtggt tgaggttgat 240tccatgccgg ctgcttcttc tgtgaagaag ccatttggtc tcaggagcaa gatgggcaag 300tggtgctgcc gttgcttccc ctgctgcagg gagagcggca agagcaacgt gggcacttct 360ggagaccacg acgactctgc tatgaagaca ctcaggagca agatgggcaa gtggtgccgc 420cactgcttcc cctgctgcag ggggagtggc aagagcaacg tgggcgcttc tggagaccac 480gacgaytctg ctatgaagac actcaggaac aagatgggca agtggtgctg ccactgcttc 540ccctgctgca gggggagcrg caagagcaag gtgggcgctt ggggagacta cgatgacagy 600gccttcatgg akcccaggta ccacgtccrt ggagaagatc tggacaagct ccacagagct 660gcctggtggg gtaaagtccc cagaaaggat ctcatcgtca tgctcaggga cackgaygtg 720aacaagargg acaagcaaaa gaggactgct ctacatctgg cctctgccaa tgggaattca 780gaagtagtaa aactcstgct ggacagacga tgtcaactta atgtccttga caacaaaaag 840aggacagctc tgayaaaggc cgtacaatgc caggaagatg aatgtgcgtt aatgttgctg 900gaacatggca ctgatccaaa tattccagat gagtatggaa ataccactct rcactaygct 960rtctayaatg aagataaatt aatggccaaa gcactgctct tatayggtgc tgatatcgaa 1020tcaaaaaaca agcatggcct cacaccactg ytacttggtr tacatgagca aaaacagcaa 1080gtsgtgaaat ttttaatyaa gaaaaaagcg aatttaaaat gcrctggata gatatggaag 1140ractgctctc atacttgctg tatgttgtgg atcagcaagt atagtcagcc ytctacttga 1200gcaaaatrtt gatgtatctt ctcaagatct ggaaagacgg ccagagagta tgctgtttct 1260agtcatcatc atgtaatttg ccagttactt tctgactaca aagaaaaaca gatgttaaaa 1320atctcttctg aaaacagcaa tccagaacaa gacttaaagc tgacatcaga ggaagagtca 1380caaaggctta aaggaagtga aaacagccag ccagaggcat ggaaactttt aaatttaaac 1440ttttggttta atgttttttt tttttgcctt aataatatta gatagtccca aatgaaatwa 1500cctatgagac taggctttga gaatcaatag attctttttt taagaatctt ttggctagga 1560gcggtgtctc acgcctgtaa ttccagcacc ttgagaggct gaggtgggca gatcacgaga 1620tcaggagatc gagaccatcc tggctaacac ggtgaaaccc catctctact aaaaatacaa 1680aaacttagct gggtgtggtg gcgggtgcct gtagtcccag ctactcagga rgctgaggca 1740ggagaatggc atgaacccgg gaggtggagg ttgcagtgag ccgagatccg ccactacact 1800ccagcctggg tgacagagca agactctgtc tcaaaaaaaa aaaaaaaaaa aaa 1853 296 2184 DNA Homo sapien 296ggcacgagaa ttaaaaccct cagcaaaaca ggcatagaag ggacatacct taaagtaata 60aaaaccacct atgacaagcc cacagccaac ataatactaa atggggaaaa gttagaagca 120tttcctctga gaactgcaac aataaataca aggatgctgg attttgtcaa atgccttttc 180tgtgtctgtt gagatgctta tgtgactttg cttttaattc tgtttatgtg attatcacat 240ttattgactt gcctgtgtta gaccggaaga gctggggtgt ttctcaggag ccaccgtgtg 300ctgcggcagc ttcgggataa cttgaggctg catcactggg gaagaaacac aytcctgtcc 360gtggcgctga tggctgagga cagagcttca gtgtggcttc tctgcgactg gcttcttcgg 420ggagttcttc cttcatagtt catccatatg gctccagagg aaaattatat tattttgtta 480tggatgaaga gtattacgtt gtgcagatat actgcagtgt cttcatctct tgatgtgtga 540ttgggtaggt tccaccatgt tgccgcagat gacatgattt cagtacctgt gtctggctga 600aaagtgtttg tttgtgaatg gatattgtgg tttctggatc tcatcctctg tgggtggaca 660gctttctcca ccttgctgga agtgacctgc tgtccagaag tttgatggct gaggagtata 720ccatcgtgca tgcatctttc atttcctgca tttcttcctc cctggatgga cagggggagc 780ggcaagagca acgtgggcac ttctggagac cacaacgact cctctgtgaa gacgcttggg 840agcaagaggt gcaagtggtg ctgccactgc ttcccctgct gcaggggagc ggcaagagca 900acgtggtcgc ttggggagac tacgatgaca gcgccttcat ggatcccagg taccacgtcc 960atggagaaga tctggacaag ctccacagag ctgcctggtg gggtaaagtc cccagaaagg 1020atctcatcgt catgctcagg gacacggatg tgaacaagag ggacaagcaa aagaggactg 1080ctctacatct ggcctctgcc aatgggaatt cagaagtagt aaaactcgtg ctggacagac 1140gatgtcaact taatgtcctt gacaacaaaa agaggacagc tctgacaaag gccgtacaat 1200gccaggaaga tgaatgtgcg ttaatgttgc tggaacatgg cactgatcca aatattccag 1260atgagtatgg aaataccact ctacactatg ctgtctacaa tgaagataaa ttaatggcca 1320aagcactgct cttatacggt gctgatatcg aatcaaaaaa caagcatggc ctcacaccac 1380tgctacttgg tatacatgag caaaaacagc aagtggtgaa atttttaatc aagaaaaaag 1440cgaatttaaa tgcgctggat agatatggaa gaactgctct catacttgct gtatgttgtg 1500gatcagcaag tatagtcagc cctctacttg agcaaaatgt tgatgtatct tctcaagatc 1560tggaaagacg gccagagagt atgctgtttc tagtcatcat catgtaattt gccagttact 1620ttctgactac aaagaaaaac agatgttaaa aatctcttct gaaaacagca atccagaaca 1680agacttaaag ctgacatcag aggaagagtc acaaaggctt aaaggaagtg aaaacagcca 1740gccagaggca tggaaacttt taaatttaaa cttttggttt aatgtttttt ttttttgcct 1800taataatatt agatagtccc aaatgaaatw acctatgaga ctaggctttg agaatcaata 1860gattcttttt ttaagaatct tttggctagg agcggtgtct cacgcctgta attccagcac 1920cttgagaggc tgaggtgggc agatcacgag atcaggagat cgagaccatc ctggctaaca 1980cggtgaaacc ccatctctac taaaaataca aaaacttagc tgggtgtggt ggcgggtgcc 2040tgtagtccca gctactcagg argctgaggc aggagaatgg catgaacccg ggaggtggag 2100gttgcagtga gccgagatcc gccactacac tccagcctgg gtgacagagc aagactctgt 2160ctcaaaaaaa aaaaaaaaaa aaaa 2184 297 1855 DNA Homo sapien misc_feature (1)...(1855) n = A,T,C or G 297tgcacgcatc ggccagtgtc tgtgccacgt acactgacgc cccctgagat gtgcacgccg 60cacgcgcacg ttgcacgcgc ggcagcggct tggctggctt gtaacggctt gcacgcgcac 120gccgcccccg cataaccgtc agactggcct gtaacggctt gcaggcgcac gccgcacgcg 180cgtaacggct tggctgccct gtaacggctt gcacgtgcat gctgcacgcg cgttaacggc 240ttggctggca tgtagccgct tggcttggct ttgcattytt tgctkggctk ggcgttgkty 300tcttggattg acgcttcctc cttggatkga cgtttcctcc ttggatkgac gtttcytyty 360tcgcgttcct ttgctggact tgacctttty tctgctgggt ttggcattcc tttggggtgg 420gctgggtgtt ttctccgggg gggktkgccc ttcctggggt gggcgtgggk cgcccccagg 480gggcgtgggc tttccccggg tgggtgtggg ttttcctggg gtggggtggg ctgtgctggg 540atccccctgc tggggttggc agggattgac ttttttcttc aaacagattg gaaacccgga 600gtaacntgct agttggtgaa actggttggt agacgcgatc tgctggtact actgtttctc 660ctggctgtta aaagcagatg gtggctgagg ttgattcaat gccggctgct tcttctgtga 720agaagccatt tggtctcagg agcaagatgg gcaagtggtg cgccactgct tcccctgctg 780cagggggagc ggcaagagca acgtgggcac ttctggagac cacaacgact cctctgtgaa 840gacgcttggg agcaagaggt gcaagtggtg ctgcccactg cttcccctgc tgcaggggag 900cggcaagagc aacgtggkcg cttggggaga ctacgatgac agcgccttca tggakcccag 960gtaccacgtc crtggagaag atctggacaa gctccacaga gctgcctggt ggggtaaagt 1020ccccagaaag gatctcatcg tcatgctcag ggacactgay gtgaacaaga rggacaagca 1080aaagaggact gctctacatc tggcctctgc caatgggaat tcagaagtag taaaactcgt 1140gctggacaga cgatgtcaac ttaatgtcct tgacaacaaa aagaggacag ctctgacaaa 1200ggccgtacaa tgccaggaag atgaatgtgc gttaatgttg ctggaacatg gcactgatcc 1260aaatattcca gatgagtatg gaaataccac tctacactat gctgtctaca atgaagataa 1320attaatggcc aaagcactgc tcttatacgg tgctgatatc gaatcaaaaa acaaggtata 1380gatctactaa ttttatcttc aaaatactga aatgcattca ttttaacatt gacgtgtgta 1440agggccagtc ttccgtattt ggaagctcaa gcataacttg aatgaaaata ttttgaaatg 1500acctaattat ctaagacttt attttaaata ttgttatttt caaagaagca ttagagggta 1560cagttttttt tttttaaatg cacttctggt aaatactttt gttgaaaaca ctgaatttgt 1620aaaaggtaat acttactatt tttcaatttt tccctcctag gatttttttc ccctaatgaa 1680tgtaagatgg caaaatttgc cctgaaatag gttttacatg aaaactccaa gaaaagttaa 1740acatgtttca gtgaatagag atcctgctcc tttggcaagt tcctaaaaaa cagtaataga 1800tacgaggtga tgcgcctgtc agtggcaagg tttaagatat ttctgatctc gtgcc 1855 298 1059 DNA Homo sapien 298gcaacgtggg cacttctgga gaccacaacg actcctctgt gaagacgctt gggagcaaga 60ggtgcaagtg gtgctgccca ctgcttcccc tgctgcaggg gagcggcaag agcaacgtgg 120gcgcttgrgg agactmcgat gacagygcct tcatggagcc caggtaccac gtccgtggag 180aagatctgga caagctccac agagctgccc tggtggggta aagtccccag aaaggatctc 240atcgtcatgc tcagggacac tgaygtgaac aagarggaca agcaaaagag gactgctcta 300catctggcct ctgccaatgg gaattcagaa gtagtaaaac tcstgctgga cagacgatgt 360caacttaatg tccttgacaa caaaaagagg acagctctga yaaaggccgt acaatgccag 420gaagatgaat gtgcgttaat gttgctggaa catggcactg atccaaatat tccagatgag 480tatggaaata ccactctrca ctaygctrtc tayaatgaag ataaattaat ggccaaagca 540ctgctcttat ayggtgctga tatcgaatca aaaaacaagg tatagatcta ctaattttat 600cttcaaaata ctgaaatgca ttcattttaa cattgacgtg tgtaagggcc agtcttccgt 660atttggaagc tcaagcataa cttgaatgaa aatattttga aatgacctaa ttatctaaga 720ctttatttta aatattgtta ttttcaaaga agcattagag ggtacagttt ttttttttta 780aatgcacttc tggtaaatac ttttgttgaa aacactgaat ttgtaaaagg taatacttac 840tatttttcaa tttttccctc ctaggatttt tttcccctaa tgaatgtaag atggcaaaat 900ttgccctgaa ataggtttta catgaaaact ccaagaaaag ttaaacatgt ttcagtgaat 960agagatcctg ctcctttggc aagttcctaa aaaacagtaa tagatacgag gtgatgcgcc 1020tgtcagtggc aaggtttaag atatttctga tctcgtgcc 1059 299 329 PRT Homo sapien 299Met Asp Ile Val Val Ser Gly Ser His Pro Leu Trp Val Asp Ser Phe 1 5 10 15Leu His Leu Ala Gly Ser Asp Leu Leu Ser Arg Ser Leu Met Ala Glu 20 25 30Glu Tyr Thr Ile Val His Ala Ser Phe Ile Ser Cys Ile Ser Ser Ser 35 40 45Leu Asp Gly Gln Gly Glu Arg Gln Glu Gln Arg Gly His Phe Trp Arg 50 55 60Pro Gln Arg Leu Leu Cys Glu Asp Ala Trp Glu Gln Glu Val Gln Val65 70 75 80Val Leu Pro Leu Leu Pro Leu Leu Gln Gly Ser Gly Lys Ser Asn Val 85 90 95Val Ala Trp Gly Asp Tyr Asp Asp Ser Ala Phe Met Asp Pro Arg Tyr 100 105 110His Val His Gly Glu Asp Leu Asp Lys Leu His Arg Ala Ala Trp Trp 115 120 125Gly Lys Val Pro Arg Lys Asp Leu Ile Val Met Leu Arg Asp Thr Asp 130 135 140Val Asn Lys Arg Asp Lys Gln Lys Arg Thr Ala Leu His Leu Ala Ser145 150 155 160Ala Asn Gly Asn Ser Glu Val Val Lys Leu Val Leu Asp Arg Arg Cys 165 170 175Gln Leu Asn Val Leu Asp Asn Lys Lys Arg Thr Ala Leu Thr Lys Ala 180 185 190Val Gln Cys Gln Glu Asp Glu Cys Ala Leu Met Leu Leu Glu His Gly 195 200 205Thr Asp Pro Asn Ile Pro Asp Glu Tyr Gly Asn Thr Thr Leu His Tyr 210 215 220Ala Val Tyr Asn Glu Asp Lys Leu Met Ala Lys Ala Leu Leu Leu Tyr225 230 235 240Gly Ala Asp Ile Glu Ser Lys Asn Lys His Gly Leu Thr Pro Leu Leu 245 250 255Leu Gly Ile His Glu Gln Lys Gln Gln Val Val Lys Phe Leu Ile Lys 260 265 270Lys Lys Ala Asn Leu Asn Ala Leu Asp Arg Tyr Gly Arg Thr Ala Leu 275 280 285Ile Leu Ala Val Cys Cys Gly Ser Ala Ser Ile Val Ser Pro Leu Leu 290 295 300Glu Gln Asn Val Asp Val Ser Ser Gln Asp Leu Glu Arg Arg Pro Glu305 310 315 320Ser Met Leu Phe Leu Val Ile Ile Met 325 300 148 PRT Homo sapien VARIANT (1)...(148) Xaa = Any Amino Acid 300Met Thr Xaa Pro Ser Trp Ser Pro Gly Thr Thr Ser Val Glu Lys Ile 1 5 10 15Trp Thr Ser Ser Thr Glu Leu Pro Trp Trp Gly Lys Val Pro Arg Lys 20 25 30Asp Leu Ile Val Met Leu Arg Asp Thr Asp Val Asn Lys Xaa Asp Lys 35 40 45Gln Lys Arg Thr Ala Leu His Leu Ala Ser Ala Asn Gly Asn Ser Glu 50 55 60Val Val Lys Leu Xaa Leu Asp Arg Arg Cys Gln Leu Asn Val Leu Asp65 70 75 80Asn Lys Lys Arg Thr Ala Leu Xaa Lys Ala Val Gln Cys Gln Glu Asp 85 90 95Glu Cys Ala Leu Met Leu Leu Glu His Gly Thr Asp Pro Asn Ile Pro 100 105 110Asp Glu Tyr Gly Asn Thr Thr Leu His Tyr Ala Xaa Tyr Asn Glu Asp 115 120 125Lys Leu Met Ala Lys Ala Leu Leu Leu Tyr Gly Ala Asp Ile Glu Ser 130 135 140Lys Asn Lys Val145 301 1155 DNA Homo sapien 301atggtggttg aggttgattc catgccggct gcctcttctg tgaagaagcc atttggtctc 60aggagcaaga tgggcaagtg gtgctgccgt tgcttcccct gctgcaggga gagcggcaag 120agcaacgtgg gcacttctgg agaccacgac gactctgcta tgaagacact caggagcaag 180atgggcaagt ggtgccgcca ctgcttcccc tgctgcaggg ggagtggcaa gagcaacgtg 240ggcgcttctg gagaccacga cgactctgct atgaagacac tcaggaacaa gatgggcaag 300tggtgctgcc actgcttccc ctgctgcagg gggagcggca agagcaaggt gggcgcttgg 360ggagactacg atgacagtgc cttcatggag cccaggtacc acgtccgtgg agaagatctg 420gacaagctcc acagagctgc ctggtggggt aaagtcccca gaaaggatct catcgtcatg 480ctcagggaca ctgacgtgaa caagaaggac aagcaaaaga ggactgctct acatctggcc 540tctgccaatg ggaattcaga agtagtaaaa ctcctgctgg acagacgatg tcaacttaat 600gtccttgaca acaaaaagag gacagctctg ataaaggccg tacaatgcca ggaagatgaa 660tgtgcgttaa tgttgctgga acatggcact gatccaaata ttccagatga gtatggaaat 720accactctgc actacgctat ctataatgaa gataaattaa tggccaaagc actgctctta 780tatggtgctg atatcgaatc aaaaaacaag catggcctca caccactgtt acttggtgta 840catgagcaaa aacagcaagt cgtgaaattt ttaatcaaga aaaaagcgaa tttaaatgca 900ctggatagat atggaaggac tgctctcata cttgctgtat gttgtggatc agcaagtata 960gtcagccttc tacttgagca aaatattgat gtatcttctc aagatctatc tggacagacg 1020gccagagagt atgctgtttc tagtcatcat catgtaattt gccagttact ttctgactac 1080aaagaaaaac agatgctaaa aatctcttct gaaaacagca atccagaaaa tgtctcaaga 1140accagaaata aataa 1155 302 2000 DNA Homo sapien 302atggtggttg aggttgattc catgccggct gcctcttctg tgaagaagcc atttggtctc 60aggagcaaga tgggcaagtg gtgctgccgt tgcttcccct gctgcaggga gagcggcaag 120agcaacgtgg gcacttctgg agaccacgac gactctgcta tgaagacact caggagcaag 180atgggcaagt ggtgccgcca ctgcttcccc tgctgcaggg ggagtggcaa gagcaacgtg 240ggcgcttctg gagaccacga cgactctgct atgaagacac tcaggaacaa gatgggcaag 300tggtgctgcc actgcttccc ctgctgcagg gggagcggca agagcaaggt gggcgcttgg 360ggagactacg atgacagtgc cttcatggag cccaggtacc acgtccgtgg agaagatctg 420gacaagctcc acagagctgc ctggtggggt aaagtcccca gaaaggatct catcgtcatg 480ctcagggaca ctgacgtgaa caagaaggac aagcaaaaga ggactgctct acatctggcc 540tctgccaatg ggaattcaga agtagtaaaa ctcctgctgg acagacgatg tcaacttaat 600gtccttgaca acaaaaagag gacagctctg ataaaggccg tacaatgcca ggaagatgaa 660tgtgcgttaa tgttgctgga acatggcact gatccaaata ttccagatga gtatggaaat 720accactctgc actacgctat ctataatgaa gataaattaa tggccaaagc actgctctta 780tatggtgctg atatcgaatc aaaaaacaag catggcctca caccactgtt acttggtgta 840catgagcaaa aacagcaagt cgtgaaattt ttaatcaaga aaaaagcgaa tttaaatgca 900ctggatagat atggaaggac tgctctcata cttgctgtat gttgtggatc agcaagtata 960gtcagccttc tacttgagca aaatattgat gtatcttctc aagatctatc tggacagacg 1020gccagagagt atgctgtttc tagtcatcat catgtaattt gccagttact ttctgactac 1080aaagaaaaac agatgctaaa aatctcttct gaaaacagca atccagaaca agacttaaag 1140ctgacatcag aggaagagtc acaaaggttc aaaggcagtg aaaatagcca gccagagaaa 1200atgtctcaag aaccagaaat aaataaggat ggtgatagag aggttgaaga agaaatgaag 1260aagcatgaaa gtaataatgt gggattacta gaaaacctga ctaatggtgt cactgctggc 1320aatggtgata atggattaat tcctcaaagg aagagcagaa cacctgaaaa tcagcaattt 1380cctgacaacg aaagtgaaga gtatcacaga atttgcgaat tagtttctga ctacaaagaa 1440aaacagatgc caaaatactc ttctgaaaac agcaacccag aacaagactt aaagctgaca 1500tcagaggaag agtcacaaag gcttgagggc agtgaaaatg gccagccaga gctagaaaat 1560tttatggcta tcgaagaaat gaagaagcac ggaagtactc atgtcggatt cccagaaaac 1620ctgactaatg gtgccactgc tggcaatggt gatgatggat taattcctcc aaggaagagc 1680agaacacctg aaagccagca atttcctgac actgagaatg aagagtatca cagtgacgaa 1740caaaatgata ctcagaagca attttgtgaa gaacagaaca ctggaatatt acacgatgag 1800attctgattc atgaagaaaa gcagatagaa gtggttgaaa aaatgaattc tgagctttct 1860cttagttgta agaaagaaaa agacatcttg catgaaaata gtacgttgcg ggaagaaatt 1920gccatgctaa gactggagct agacacaatg aaacatcaga gccagctaaa aaaaaaaaaa 1980aaaaaaaaaa aaaaaaaaaa 2000 303 2040 DNA Homo sapien 303atggtggttg aggttgattc catgccggct gcctcttctg tgaagaagcc atttggtctc 60aggagcaaga tgggcaagtg gtgctgccgt tgcttcccct gctgcaggga gagcggcaag 120agcaacgtgg gcacttctgg agaccacgac gactctgcta tgaagacact caggagcaag 180atgggcaagt ggtgccgcca ctgcttcccc tgctgcaggg ggagtggcaa gagcaacgtg 240ggcgcttctg gagaccacga cgactctgct atgaagacac tcaggaacaa gatgggcaag 300tggtgctgcc actgcttccc ctgctgcagg gggagcggca agagcaaggt gggcgcttgg 360ggagactacg atgacagtgc cttcatggag cccaggtacc acgtccgtgg agaagatctg 420gacaagctcc acagagctgc ctggtggggt aaagtcccca gaaaggatct catcgtcatg 480ctcagggaca ctgacgtgaa caagaaggac aagcaaaaga ggactgctct acatctggcc 540tctgccaatg ggaattcaga agtagtaaaa ctcctgctgg acagacgatg tcaacttaat 600gtccttgaca acaaaaagag gacagctctg ataaaggccg tacaatgcca ggaagatgaa 660tgtgcgttaa tgttgctgga acatggcact gatccaaata ttccagatga gtatggaaat 720accactctgc actacgctat ctataatgaa gataaattaa tggccaaagc actgctctta 780tatggtgctg atatcgaatc aaaaaacaag catggcctca caccactgtt acttggtgta 840catgagcaaa aacagcaagt cgtgaaattt ttaatcaaga aaaaagcgaa tttaaatgca 900ctggatagat atggaaggac tgctctcata cttgctgtat gttgtggatc agcaagtata 960gtcagccttc tacttgagca aaatattgat gtatcttctc aagatctatc tggacagacg 1020gccagagagt atgctgtttc tagtcatcat catgtaattt gccagttact ttctgactac 1080aaagaaaaac agatgctaaa aatctcttct gaaaacagca atccagaaca agacttaaag 1140ctgacatcag aggaagagtc acaaaggttc aaaggcagtg aaaatagcca gccagagaaa 1200atgtctcaag aaccagaaat aaataaggat ggtgatagag aggttgaaga agaaatgaag 1260aagcatgaaa gtaataatgt gggattacta gaaaacctga ctaatggtgt cactgctggc 1320aatggtgata atggattaat tcctcaaagg aagagcagaa cacctgaaaa tcagcaattt 1380cctgacaacg aaagtgaaga gtatcacaga atttgcgaat tagtttctga ctacaaagaa 1440aaacagatgc caaaatactc ttctgaaaac agcaacccag aacaagactt aaagctgaca 1500tcagaggaag agtcacaaag gcttgagggc agtgaaaatg gccagccaga gaaaagatct 1560caagaaccag aaataaataa ggatggtgat agagagctag aaaattttat ggctatcgaa 1620gaaatgaaga agcacggaag tactcatgtc ggattcccag aaaacctgac taatggtgcc 1680actgctggca atggtgatga tggattaatt cctccaagga agagcagaac acctgaaagc 1740cagcaatttc ctgacactga gaatgaagag tatcacagtg acgaacaaaa tgatactcag 1800aagcaatttt gtgaagaaca gaacactgga atattacacg atgagattct gattcatgaa 1860gaaaagcaga tagaagtggt tgaaaaaatg aattctgagc tttctcttag ttgtaagaaa 1920gaaaaagaca tcttgcatga aaatagtacg ttgcgggaag aaattgccat gctaagactg 1980gagctagaca caatgaaaca tcagagccag ctaaaaaaaa aaaaaaaaaa aaaaaaaaaa 2040 304 384 PRT Homo sapien 304Met Val Val Glu Val Asp Ser Met Pro Ala Ala Ser Ser Val Lys Lys 1 5 10 15Pro Phe Gly Leu Arg Ser Lys Met Gly Lys Trp Cys Cys Arg Cys Phe 20 25 30Pro Cys Cys Arg Glu Ser Gly Lys Ser Asn Val Gly Thr Ser Gly Asp 35 40 45His Asp Asp Ser Ala Met Lys Thr Leu Arg Ser Lys Met Gly Lys Trp 50 55 60Cys Arg His Cys Phe Pro Cys Cys Arg Gly Ser Gly Lys Ser Asn Val65 70 75 80Gly Ala Ser Gly Asp His Asp Asp Ser Ala Met Lys Thr Leu Arg Asn 85 90 95Lys Met Gly Lys Trp Cys Cys His Cys Phe Pro Cys Cys Arg Gly Ser 100 105 110Gly Lys Ser Lys Val Gly Ala Trp Gly Asp Tyr Asp Asp Ser Ala Phe 115 120 125Met Glu Pro Arg Tyr His Val Arg Gly Glu Asp Leu Asp Lys Leu His 130 135 140Arg Ala Ala Trp Trp Gly Lys Val Pro Arg Lys Asp Leu Ile Val Met145 150 155 160Leu Arg Asp Thr Asp Val Asn Lys Lys Asp Lys Gln Lys Arg Thr Ala 165 170 175Leu His Leu Ala Ser Ala Asn Gly Asn Ser Glu Val Val Lys Leu Leu 180 185 190Leu Asp Arg Arg Cys Gln Leu Asn Val Leu Asp Asn Lys Lys Arg Thr 195 200 205Ala Leu Ile Lys Ala Val Gln Cys Gln Glu Asp Glu Cys Ala Leu Met 210 215 220Leu Leu Glu His Gly Thr Asp Pro Asn Ile Pro Asp Glu Tyr Gly Asn225 230 235 240Thr Thr Leu His Tyr Ala Ile Tyr Asn Glu Asp Lys Leu Met Ala Lys 245 250 255Ala Leu Leu Leu Tyr Gly Ala Asp Ile Glu Ser Lys Asn Lys His Gly 260 265 270Leu Thr Pro Leu Leu Leu Gly Val His Glu Gln Lys Gln Gln Val Val 275 280 285Lys Phe Leu Ile Lys Lys Lys Ala Asn Leu Asn Ala Leu Asp Arg Tyr 290 295 300Gly Arg Thr Ala Leu Ile Leu Ala Val Cys Cys Gly Ser Ala Ser Ile305 310 315 320Val Ser Leu Leu Leu Glu Gln Asn Ile Asp Val Ser Ser Gln Asp Leu 325 330 335Ser Gly Gln Thr Ala Arg Glu Tyr Ala Val Ser Ser His His His Val 340 345 350Ile Cys Gln Leu Leu Ser Asp Tyr Lys Glu Lys Gln Met Leu Lys Ile 355 360 365Ser Ser Glu Asn Ser Asn Pro Glu Asn Val Ser Arg Thr Arg Asn Lys 370 375 380 305 656 PRT Homo sapien 305Met Val Val Glu Val Asp Ser Met Pro Ala Ala Ser Ser Val Lys Lys 1 5 10 15Pro Phe Gly Leu Arg Ser Lys Met Gly Lys Trp Cys Cys Arg Cys Phe 20 25 30Pro Cys Cys Arg Glu Ser Gly Lys Ser Asn Val Gly Thr Ser Gly Asp 35 40 45His Asp Asp Ser Ala Met Lys Thr Leu Arg Ser Lys Met Gly Lys Trp 50 55 60Cys Arg His Cys Phe Pro Cys Cys Arg Gly Ser Gly Lys Ser Asn Val65 70 75 80Gly Ala Ser Gly Asp His Asp Asp Ser Ala Met Lys Thr Leu Arg Asn 85 90 95Lys Met Gly Lys Trp Cys Cys His Cys Phe Pro Cys Cys Arg Gly Ser 100 105 110Gly Lys Ser Lys Val Gly Ala Trp Gly Asp Tyr Asp Asp Ser Ala Phe 115 120 125Met Glu Pro Arg Tyr His Val Arg Gly Glu Asp Leu Asp Lys Leu His 130 135 140Arg Ala Ala Trp Trp Gly Lys Val Pro Arg Lys Asp Leu Ile Val Met145 150 155 160Leu Arg Asp Thr Asp Val Asn Lys Lys Asp Lys Gln Lys Arg Thr Ala 165 170 175Leu His Leu Ala Ser Ala Asn Gly Asn Ser Glu Val Val Lys Leu Leu 180 185 190Leu Asp Arg Arg Cys Gln Leu Asn Val Leu Asp Asn Lys Lys Arg Thr 195 200 205Ala Leu Ile Lys Ala Val Gln Cys Gln Glu Asp Glu Cys Ala Leu Met 210 215 220Leu Leu Glu His Gly Thr Asp Pro Asn Ile Pro Asp Glu Tyr Gly Asn225 230 235 240Thr Thr Leu His Tyr Ala Ile Tyr Asn Glu Asp Lys Leu Met Ala Lys 245 250 255Ala Leu Leu Leu Tyr Gly Ala Asp Ile Glu Ser Lys Asn Lys His Gly 260 265 270Leu Thr Pro Leu Leu Leu Gly Val His Glu Gln Lys Gln Gln Val Val 275 280 285Lys Phe Leu Ile Lys Lys Lys Ala Asn Leu Asn Ala Leu Asp Arg Tyr 290 295 300Gly Arg Thr Ala Leu Ile Leu Ala Val Cys Cys Gly Ser Ala Ser Ile305 310 315 320Val Ser Leu Leu Leu Glu Gln Asn Ile Asp Val Ser Ser Gln Asp Leu 325 330 335Ser Gly Gln Thr Ala Arg Glu Tyr Ala Val Ser Ser His His His Val 340 345 350Ile Cys Gln Leu Leu Ser Asp Tyr Lys Glu Lys Gln Met Leu Lys Ile 355 360 365Ser Ser Glu Asn Ser Asn Pro Glu Gln Asp Leu Lys Leu Thr Ser Glu 370 375 380Glu Glu Ser Gln Arg Phe Lys Gly Ser Glu Asn Ser Gln Pro Glu Lys385 390 395 400Met Ser Gln Glu Pro Glu Ile Asn Lys Asp Gly Asp Arg Glu Val Glu 405 410 415Glu Glu Met Lys Lys His Glu Ser Asn Asn Val Gly Leu Leu Glu Asn 420 425 430Leu Thr Asn Gly Val Thr Ala Gly Asn Gly Asp Asn Gly Leu Ile Pro 435 440 445Gln Arg Lys Ser Arg Thr Pro Glu Asn Gln Gln Phe Pro Asp Asn Glu 450 455 460Ser Glu Glu Tyr His Arg Ile Cys Glu Leu Val Ser Asp Tyr Lys Glu465 470 475 480Lys Gln Met Pro Lys Tyr Ser Ser Glu Asn Ser Asn Pro Glu Gln Asp 485 490 495Leu Lys Leu Thr Ser Glu Glu Glu Ser Gln Arg Leu Glu Gly Ser Glu 500 505 510Asn Gly Gln Pro Glu Leu Glu Asn Phe Met Ala Ile Glu Glu Met Lys 515 520 525Lys His Gly Ser Thr His Val Gly Phe Pro Glu Asn Leu Thr Asn Gly 530 535 540Ala Thr Ala Gly Asn Gly Asp Asp Gly Leu Ile Pro Pro Arg Lys Ser545 550 555 560Arg Thr Pro Glu Ser Gln Gln Phe Pro Asp Thr Glu Asn Glu Glu Tyr 565 570 575His Ser Asp Glu Gln Asn Asp Thr Gln Lys Gln Phe Cys Glu Glu Gln 580 585 590Asn Thr Gly Ile Leu His Asp Glu Ile Leu Ile His Glu Glu Lys Gln 595 600 605Ile Glu Val Val Glu Lys Met Asn Ser Glu Leu Ser Leu Ser Cys Lys 610 615 620Lys Glu Lys Asp Ile Leu His Glu Asn Ser Thr Leu Arg Glu Glu Ile625 630 635 640Ala Met Leu Arg Leu Glu Leu Asp Thr Met Lys His Gln Ser Gln Leu 645 650 655 306 671 PRT Homo sapien 306Met Val Val Glu Val Asp Ser Met Pro Ala Ala Ser Ser Val Lys Lys 1 5 10 15Pro Phe Gly Leu Arg Ser Lys Met Gly Lys Trp Cys Cys Arg Cys Phe 20 25 30Pro Cys Cys Arg Glu Ser Gly Lys Ser Asn Val Gly Thr Ser Gly Asp 35 40 45His Asp Asp Ser Ala Met Lys Thr Leu Arg Ser Lys Met Gly Lys Trp 50 55 60Cys Arg His Cys Phe Pro Cys Cys Arg Gly Ser Gly Lys Ser Asn Val65 70 75 80Gly Ala Ser Gly Asp His Asp Asp Ser Ala Met Lys Thr Leu Arg Asn 85 90 95Lys Met Gly Lys Trp Cys Cys His Cys Phe Pro Cys Cys Arg Gly Ser 100 105 110Gly Lys Ser Lys Val Gly Ala Trp Gly Asp Tyr Asp Asp Ser Ala Phe 115 120 125Met Glu Pro Arg Tyr His Val Arg Gly Glu Asp Leu Asp Lys Leu His 130 135 140Arg Ala Ala Trp Trp Gly Lys Val Pro Arg Lys Asp Leu Ile Val Met145 150 155 160Leu Arg Asp Thr Asp Val Asn Lys Lys Asp Lys Gln Lys Arg Thr Ala 165 170 175Leu His Leu Ala Ser Ala Asn Gly Asn Ser Glu Val Val Lys Leu Leu 180 185 190Leu Asp Arg Arg Cys Gln Leu Asn Val Leu Asp Asn Lys Lys Arg Thr 195 200 205Ala Leu Ile Lys Ala Val Gln Cys Gln Glu Asp Glu Cys Ala Leu Met 210 215 220Leu Leu Glu His Gly Thr Asp Pro Asn Ile Pro Asp Glu Tyr Gly Asn225 230 235 240Thr Thr Leu His Tyr Ala Ile Tyr Asn Glu Asp Lys Leu Met Ala Lys 245 250 255Ala Leu Leu Leu Tyr Gly Ala Asp Ile Glu Ser Lys Asn Lys His Gly 260 265 270Leu Thr Pro Leu Leu Leu Gly Val His Glu Gln Lys Gln Gln Val Val 275 280 285Lys Phe Leu Ile Lys Lys Lys Ala Asn Leu Asn Ala Leu Asp Arg Tyr 290 295 300Gly Arg Thr Ala Leu Ile Leu Ala Val Cys Cys Gly Ser Ala Ser Ile305 310 315 320Val Ser Leu Leu Leu Glu Gln Asn Ile Asp Val Ser Ser Gln Asp Leu 325 330 335Ser Gly Gln Thr Ala Arg Glu Tyr Ala Val Ser Ser His His His Val 340 345 350Ile Cys Gln Leu Leu Ser Asp Tyr Lys Glu Lys Gln Met Leu Lys Ile 355 360 365Ser Ser Glu Asn Ser Asn Pro Glu Gln Asp Leu Lys Leu Thr Ser Glu 370 375 380Glu Glu Ser Gln Arg Phe Lys Gly Ser Glu Asn Ser Gln Pro Glu Lys385 390 395 400Met Ser Gln Glu Pro Glu Ile Asn Lys Asp Gly Asp Arg Glu Val Glu 405 410 415Glu Glu Met Lys Lys His Glu Ser Asn Asn Val Gly Leu Leu Glu Asn 420 425 430Leu Thr Asn Gly Val Thr Ala Gly Asn Gly Asp Asn Gly Leu Ile Pro 435 440 445Gln Arg Lys Ser Arg Thr Pro Glu Asn Gln Gln Phe Pro Asp Asn Glu 450 455 460Ser Glu Glu Tyr His Arg Ile Cys Glu Leu Val Ser Asp Tyr Lys Glu465 470 475 480Lys Gln Met Pro Lys Tyr Ser Ser Glu Asn Ser Asn Pro Glu Gln Asp 485 490 495Leu Lys Leu Thr Ser Glu Glu Glu Ser Gln Arg Leu Glu Gly Ser Glu 500 505 510Asn Gly Gln Pro Glu Lys Arg Ser Gln Glu Pro Glu Ile Asn Lys Asp 515 520 525Gly Asp Arg Glu Leu Glu Asn Phe Met Ala Ile Glu Glu Met Lys Lys 530 535 540His Gly Ser Thr His Val Gly Phe Pro Glu Asn Leu Thr Asn Gly Ala545 550 555 560Thr Ala Gly Asn Gly Asp Asp Gly Leu Ile Pro Pro Arg Lys Ser Arg 565 570 575Thr Pro Glu Ser Gln Gln Phe Pro Asp Thr Glu Asn Glu Glu Tyr His 580 585 590Ser Asp Glu Gln Asn Asp Thr Gln Lys Gln Phe Cys Glu Glu Gln Asn 595 600 605Thr Gly Ile Leu His Asp Glu Ile Leu Ile His Glu Glu Lys Gln Ile 610 615 620Glu Val Val Glu Lys Met Asn Ser Glu Leu Ser Leu Ser Cys Lys Lys625 630 635 640Glu Lys Asp Ile Leu His Glu Asn Ser Thr Leu Arg Glu Glu Ile Ala 645 650 655Met Leu Arg Leu Glu Leu Asp Thr Met Lys His Gln Ser Gln Leu 660 665 670 307 800 DNA Homo sapien 307atkagcttcc gcttctgaca acactagaga tccctcccct ccctcagggt atggccctcc 60acttcatttt tggtacataa catctttata ggacaggggt aaaatcccaa tactaacagg 120agaatgctta ggactctaac aggtttttga gaatgtgttg gtaagggcca ctcaatccaa 180tttttcttgg tcctccttgt ggtctaggag gacaggcaag ggtgcagatt ttcaagaatg 240catcagtaag ggccactaaa tccgaccttc ctcgttcctc cttgtggtct gggaggaaaa 300ctagtgtttc tgttgctgtg tcagtgagca caactattcc gatcagcagg gtccagggac 360cactgcaggt tcttgggcag ggggagaaac aaaacaaacc aaaaccatgg gcrgttttgt 420ctttcagatg ggaaacactc aggcatcaac aggctcacct ttgaaatgca tcctaagcca 480atgggacaaa tttgacccac aaaccctgga aaaagaggtg gctcattttt tttgcactat 540ggcttggccc caacattctc tctctgatgg ggaaaaatgg ccacctgagg gaagtacaga 600ttacaatact atcctgcagc ttgacctttt ctgtaagagg gaaggcaaat ggagtgaaat 660accttatgtc caagctttct tttcattgaa ggagaataca ctatgcaaag cttgaaattt 720acatcccaca ggaggacctc tcagcttacc cccatatcct agcctcccta tagctcccct 780tcctattagt gataagcctc 800 308 102 PRT Homo sapien VARIANT (1)...(102) Xaa = Any Amino Acid 308Met Gly Xaa Phe Val Phe Gln Met Gly Asn Thr Gln Ala Ser Thr Gly 1 5 10 15Ser Pro Leu Lys Cys Ile Leu Ser Gln Trp Asp Lys Phe Asp Pro Gln 20 25 30Thr Leu Glu Lys Glu Val Ala His Phe Phe Cys Thr Met Ala Trp Pro 35 40 45Gln His Ser Leu Ser Asp Gly Glu Lys Trp Pro Pro Glu Gly Ser Thr 50 55 60Asp Tyr Asn Thr Ile Leu Gln Leu Asp Leu Phe Cys Lys Arg Glu Gly65 70 75 80Lys Trp Ser Glu Ile Pro Tyr Val Gln Ala Phe Phe Ser Leu Lys Glu 85 90 95Asn Thr Leu Cys Lys Ala 100 309 9 PRT Artificial Sequence Made in the lab 309Leu Met Ala Glu Glu Tyr Thr Ile Val 1 5 310 9 PRT Artificial Sequence Made in the lab 310Lys Leu Met Ala Lys Ala Leu Leu Leu 1 5 311 9 PRT Artificial Sequence Made in the lab 311Gly Leu Thr Pro Leu Leu Leu Gly Ile 1 5 312 10 PRT Artificial Sequence Made in the lab 312Lys Leu Val Leu Asp Arg Arg Cys Gln Leu 1 5 10 313 1852 DNA Homo sapiens 313ggcacgagaa ttaaaaccct cagcaaaaca ggcatagaag ggacatacct taaagtaata 60aaaaccacct atgacaagcc cacagccaac ataatactaa atggggaaaa gttagaagca 120tttcctctga gaactgcaac aataaataca aggatgctgg attttgtcaa atgccttttc 180tgtgtctgtt gagatgctta tgtgactttg cttttaattc tgtttatgtg attatcacat 240ttattgactt gcctgtgtta gaccggaaga gctggggtgt ttctcaggag ccaccgtgtg 300ctgcggcagc ttcgggataa cttgaggctg catcactggg gaagaaacac aytcctgtcc 360gtggcgctga tggctgagga cagagcttca gtgtggcttc tctgcgactg gcttcttcgg 420ggagttcttc cttcatagtt catccatatg gctccagagg aaaattatat tattttgtta 480tggatgaaga gtattacgtt gtgcagatat actgcagtgt cttcatctct tgatgtgtga 540ttgggtaggt tccaccatgt tgccgcagat gacatgattt cagtacctgt gtctggctga 600aaagtgtttg tttgtgaatg gatattgtgg tttctggatc tcatcctctg tgggtggaca 660gctttctcca ccttgctgga agtgacctgc tgtccagaag tttgatggct gaggagtata 720ccatcgtgca tgcatctttc atttcctgca tttcttcctc cctggatgga cagggggagc 780ggcaagagca acgtgggcac ttctggagac cacaacgact cctctgtgaa gacgcttggg 840agcaagaggt gcaagtggtg ctgccactgc ttcccctgct gcagggggag cggcaagagc 900aacgtggtcg cttggggaga ctacgatgac agcgccttca tggatcccag gtaccacgtc 960catggagaag atctggacaa gctccacaga gctgcctggt ggggtaaagt ccccagaaag 1020gatctcatcg tcatgctcag ggacacggat gtgaacaaga gggacaagca aaagaggact 1080gctctacatc tggcctctgc caatgggaat tcagaagtag taaaactcgt gctggacaga 1140cgatgtcaac ttaatgtcct tgacaacaaa aagaggacag ctctgacaaa ggccgtacaa 1200tgccaggaag atgaatgtgc gttaatgttg ctggaacatg gcactgatcc aaatattcca 1260gatgagtatg gaaataccac tctacactat gctgtctaca atgaagataa attaatggcc 1320aaagcactgc tcttatacgg tgctgatatc gaatcaaaaa acaagcatgg cctcacacca 1380ctgctacttg gtatacatga gcaaaaacag caagtggtga aatttttaat caagaaaaaa 1440gcgaatttaa atgcgctgga tagatatgga agaactgctc tcatacttgc tgtatgttgt 1500ggatcagcaa gtatagtcag ccctctactt gagcaaaatg ttgatgtatc ttctcaagat 1560ctggaaagac ggccagagag tatgctgttt ctagtcatca tcatgtaatt tgccagttac 1620tttctgacta caaagaaaaa cagatgttaa aaatctcttc tgaaaacagc aatccagaac 1680aagacttaaa gctgacatca gaggaagagt cacaaaggct taaaggaagt gaaaacagcc 1740agccagagct agaagattta tggctattga agaagaatga agaacacgga agtactcatg 1800tgggattccc agaaaacctg actaacggtg ccgctgctgg caatggtgat ga 1852 314 879 DNA Homo sapiens 314atgcatcttt catttcctgc atttcttcct ccctggatgg acagggggag cggcaagagc 60aacgtgggca cttctggaga ccacaacgac tcctctgtga agacgcttgg gagcaagagg 120tgcaagtggt gctgccactg cttcccctgc tgcaggggga gcggcaagag caacgtggtc 180gcttggggag actacgatga cagcgccttc atggatccca ggtaccacgt ccatggagaa 240gatctggaca agctccacag agctgcctgg tggggtaaag tccccagaaa ggatctcatc 300gtcatgctca gggacacgga tgtgaacaag agggacaagc aaaagaggac tgctctacat 360ctggcctctg ccaatgggaa ttcagaagta gtaaaactcg tgctggacag acgatgtcaa 420cttaatgtcc ttgacaacaa aaagaggaca gctctgacaa aggccgtaca atgccaggaa 480gatgaatgtg cgttaatgtt gctggaacat ggcactgatc caaatattcc agatgagtat 540ggaaatacca ctctacacta tgctgtctac aatgaagata aattaatggc caaagcactg 600ctcttatacg gtgctgatat cgaatcaaaa aacaagcatg gcctcacacc actgctactt 660ggtatacatg agcaaaaaca gcaagtggtg aaatttttaa tcaagaaaaa agcgaattta 720aatgcgctgg atagatatgg aagaactgct ctcatacttg ctgtatgttg tggatcagca 780agtatagtca gccctctact tgagcaaaat gttgatgtat cttctcaaga tctggaaaga 840cggccagaga gtatgctgtt tctagtcatc atcatgtaa 879 315 292 PRT Homo sapiens 315Met His Leu Ser Phe Pro Ala Phe Leu Pro Pro Trp Met Asp Arg Gly 5 10 15Ser Gly Lys Ser Asn Val Gly Thr Ser Gly Asp His Asn Asp Ser Ser 20 25 30Val Lys Thr Leu Gly Ser Lys Arg Cys Lys Trp Cys Cys His Cys Phe 35 40 45Pro Cys Cys Arg Gly Ser Gly Lys Ser Asn Val Val Ala Trp Gly Asp 50 55 60Tyr Asp Asp Ser Ala Phe Met Asp Pro Arg Tyr His Val His Gly Glu 65 70 75 80Asp Leu Asp Lys Leu His Arg Ala Ala Trp Trp Gly Lys Val Pro Arg 85 90 95Lys Asp Leu Ile Val Met Leu Arg Asp Thr Asp Val Asn Lys Arg Asp 100 105 110Lys Gln Lys Arg Thr Ala Leu His Leu Ala Ser Ala Asn Gly Asn Ser 115 120 125Glu Val Val Lys Leu Val Leu Asp Arg Arg Cys Gln Leu Asn Val Leu 130 135 140Asp Asn Lys Lys Arg Thr Ala Leu Thr Lys Ala Val Gln Cys Gln Glu145 150 155 160Asp Glu Cys Ala Leu Met Leu Leu Glu His Gly Thr Asp Pro Asn Ile 165 170 175Pro Asp Glu Tyr Gly Asn Thr Thr Leu His Tyr Ala Val Tyr Asn Glu 180 185 190Asp Lys Leu Met Ala Lys Ala Leu Leu Leu Tyr Gly Ala Asp Ile Glu 195 200 205Ser Lys Asn Lys His Gly Leu Thr Pro Leu Leu Leu Gly Ile His Glu 210 215 220Gln Lys Gln Gln Val Val Lys Phe Leu Ile Lys Lys Lys Ala Asn Leu225 230 235 240Asn Ala Leu Asp Arg Tyr Gly Arg Thr Ala Leu Ile Leu Ala Val Cys 245 250 255Cys Gly Ser Ala Ser Ile Val Ser Pro Leu Leu Glu Gln Asn Val Asp 260 265 270Val Ser Ser Gln Asp Leu Glu Arg Arg Pro Glu Ser Met Leu Phe Leu 275 280 285Val Ile Ile Met 290

Claims

1. An isolated polypeptide comprising an amino acid sequence of SEQ ID NO: 315.

2. A composition, comprising a polypeptide according to claim 1, and a physiologically acceptable carrier.

3. A composition comprising a polypeptide according to claim 1, and an immune response enhancer.

4. An isolated polypeptide comprising an amino acid sequence encoded by a nucleotide sequence selected from the group consisting of:(a) SEQ ID NO: 313; and (b) SEQ ID NO: 314.

5. An isolated polypeptide comprising an amino acid sequence encoded by a nucleotide sequence selected from the group consisting of sequences that have at least 80% identity to any one of SEQ ID NO: 313 and 314, wherein the polypeptide is expressed in breast tumor tissue at a level that is at least two times the level of expression in normal breast tissue.

6. A composition comprising a polypeptide according to any one of claims 4 or 5, and a physiologically acceptable carrier.

7. A composition comprising a polypeptide according to any one of claims 4 or 5, and an immune response enhancer.

8. An isolated polypeptide comprising an amino acid sequence encoded by a nucleotide sequence selected from the group consisting of sequences that have at least 90% identity to any one of SEQ ID NO:313 and 314; wherein the polypeptide is expressed in breast tumor tissue at a level that is at least two times the level of expression in normal breast tissue.