Compositions and methods for therapy of ovarian cancer

Abstract

Compositions and methods for the therapy of cancer, such as ovarian cancer, are disclosed. Compositions may comprise one or more ovarian carcinoma proteins, immunogenic portions thereof, polynucleotides that encode such portions or antibodies or immune system cells specific for such proteins. Such compositions may be used, for example, for the prevention and treatment of diseases such as ovarian cancer. Methods are further provided for identifying tumor antigens that are secreted from ovarian carcinomas and/or other tumors.

Description

TECHNICAL FIELD

The present invention relates generally to ovarian cancer therapy. The invention is more specifically related to polypeptides comprising at least a portion of an ovarian carcinoma protein, and to polynucleotides encoding such polypeptides, as well as antibodies and immune system cells that specifically recognize such polypeptides. Such polypeptides, polynucleotides antibodies and cells may be used in vaccines and pharmaceutical compositions for treatment of ovarian cancer.

BACKGROUND OF THE INVENTION

Ovarian cancer is a significant health problem for women in the United States and throughout the world. Although advances have been made in detection and therapy of this cancer, no vaccine or other universally successful method for prevention or treatment is currently available. Management of the disease currently relies on a combination of early diagnosis and aggressive treatment, which may include one or more of a variety of treatments such as surgery, radiotherapy, chemotherapy and hormone therapy. The course of treatment for a particular cancer is often selected based on a variety of prognostic parameters, including an analysis of specific tumor markers. However, the use of established markers often leads to a result that is difficult to interpret, and high mortality continues to be observed in many cancer patients.

Immunotherapies have the potential to substantially improve cancer treatment and survival. Such therapies may involve the generation or enhancement of an immune response to an ovarian carcinoma antigen. However, to date, relatively few ovarian carcinoma antigens are known and the generation of an immune response against such antigens has not been shown to be therapeutically beneficial.

Accordingly, there is a need in the art for improved methods for identifying ovarian tumor antigens and for using such antigens in the therapy of ovarian cancer. The present invention fulfills these needs and further provides other related advantages.

SUMMARY OF THE INVENTION

Briefly stated, this invention provides compositions and methods for the therapy of cancer, such as ovarian cancer. In one aspect, the present invention provides polypeptides comprising an immunogenic portion of an ovarian carcinoma protein, or a variant thereof that differs in one or more substitutions, deletions, additions and/or insertions such that the ability of the variant to react with ovarian carcinoma protein-specific antisera is not substantially diminished. Within certain embodiments, the polypeptide comprises a sequence that is encoded by a polynucleotide sequence selected from the group consisting of SEQ ID NOs:1-81, and complements of such polynucleotides.

The present invention further provides polynucleotides that encode a polypeptide as described above or a portion thereof, expression vectors comprising such polynucleotides and host cells transformed or transfected with such expression vectors.

Within other aspects, the present invention provides pharmaceutical compositions and vaccines. Pharmaceutical compositions may comprise a physiologically acceptable carrier or excipient in combination with one or more of: (i) a polypeptide comprising an immunogenic portion of an ovarian carcinoma protein, or a variant thereof that differs in one or more substitutions, deletions, additions and/or insertions such that the ability of the variant to react with ovarian carcinoma protein- specific antisera is not substantially diminished, wherein the ovarian carcinoma protein comprises an amino acid sequence encoded by a polynucleotide that comprises a sequence recited in any one of SEQ ID NOs: 1-81; (ii) a polynucleotide encoding such a polypeptide; (iii) an antibody that specifically binds to such a polypeptide; (iv) an antigen-presenting cell that expresses such a polypeptide and/or (v) a T cell that specifically reacts with such a polypeptide. Vaccines may comprise a non-specific immune response enhancer in combination with one or more of: (i) a polypeptide comprising an immunogenic portion of an ovarian carcinoma protein, or a variant thereof that differs in one or more substitutions, deletions, additions and/or insertions such that the ability of the variant to react with ovarian carcinoma protein-specific antisera is not substantially diminished, wherein the ovarian carcinoma protein comprises an amino acid sequence encoded by a polynucleotide that comprises a sequence recited in any one of SEQ ID NOs: 1-81, (ii) a polynucleotide encoding such a polypeptide; (iii) an anti-idiotypic antibody that is specifically bound by an antibody that specifically binds to such a polypeptide; (iv) an antigen-presenting cell that expresses such a polypeptide and/or (v) a T cell that specifically reacts with such a polypeptide.

The present invention further provides, in other aspects, fusion proteins that comprise at least one polypeptide as described above, as well as polynucleotides encoding such fusion proteins.

Within related aspects, pharmaceutical compositions comprising a fusion protein or polynucleotide encoding a fusion protein in combination with a physiologically acceptable carrier are provided.

Vaccines are further provided, within other aspects, comprising a fusion protein or polynucleotide encoding a fusion protein in combination with a non-specific immune response enhancer.

Within further aspects, the present invention provides methods for inhibiting the development of a cancer in a patient, comprising administering to a patient a pharmaceutical composition or vaccine as recited above.

The present invention further provides, within other aspects, methods for stimulating and/or expanding T cells, comprising contacting T cells with (a) a polypeptide comprising an immunogenic portion of an ovarian carcinoma protein, or a variant thereof that differs in one or more substitutions, deletions, additions and/or insertions such that the ability of the variant to react with ovarian carcinoma protein-specific antisera is not substantially diminished, wherein the ovarian carcinoma protein comprises an amino acid sequence encoded by a polynucleotide that comprises a sequence recited in any one of SEQ ID NOs: 1-310; (b) a polynucleotide encoding such a polypeptide and/or (c) an antigen presenting cell that expresses such a polypeptide under conditions and for a time sufficient to permit the stimulation and/or expansion of T cells. Such polypeptide, polynucleotide and/or antigen presenting cell(s) may be present within a pharmaceutical composition or vaccine, for use in stimulating and/or expanding T cells in a mammal.

Within other aspects, the present invention provides methods for inhibiting the development of ovarian cancer in a patient, comprising administering to a patient T cells prepared as described above.

Within further aspects, the present invention provides methods for inhibiting the development of ovarian cancer in a patient, comprising the steps of: (a) incubating CD4 + and/or CD8 + T cells isolated from a patient with one or more of: (i) a polypeptide comprising an immunogenic portion of an ovarian carcinoma protein, or a variant thereof that differs in one or more substitutions, deletions, additions and/or insertions such that the ability of the variant to react with ovarian carcinoma protein-specific antisera is not substantially diminished, wherein the ovarian carcinoma protein comprises an amino acid sequence encoded by a polynucleotide that comprises a sequence recited in any one of SEQ ID NOs: 1-310; (ii) a polynucleotide encoding such a polypeptide; or (iii) an antigen-presenting cell that expresses such a polypeptide; such that T cells proliferate; and (b) administering to the patient an effective amount of the proliferated T cells, and thereby inhibiting the development of ovarian cancer in the patient. The proliferated cells may be cloned prior to administration to the patient.

The present invention also provides, within other aspects, methods for identifying secreted tumor antigens. Such methods comprise the steps of: (a) implanting tumor cells in an immunodeficient mammal; (b) obtaining serum from the immunodeficient mammal after a time sufficient to permit secretion of tumor antigens into the serum; (c) immunizing an immunocompetent mammal with the serum; (d) obtaining antiserum from the immunocompetent mammal; and (e) screening a tumor expression library with the antiserum, and therefrom identifying a secreted tumor antigen. A preferred method for identifying a secreted ovarian carcinoma antigen comprises the steps of: (a) implanting ovarian carcinoma cells in a SCID mouse; (b) obtaining serum from the SCID mouse after a time sufficient to permit secretion of ovarian carcinoma antigens into the serum; (c) immunizing an immunocompetent mouse with the serum; (d) obtaining antiserum from the immunocompetent mouse; and (e) screening an ovarian carcinoma expression library with the antiserum, and therefrom identifying a secreted ovarian carcinoma antigen.

These and other aspects of the present invention will become apparent upon reference to the following detailed description and attached drawings. All references disclosed herein are hereby incorporated by reference in their entirety as if each was incorporated individually.

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1A-1S (SEQ ID NOs:1-71) depict partial sequences of polynucleotides encoding representative secreted ovarian carcinoma antigens.

FIGS. 2A-2C depict full insert sequences for three of the clones of FIG. 1 . FIG. 2A shows the sequence designated O7E (11731; SEQ ID NO:72), FIG. 2B shows the sequence designated O9E (11785; SEQ ID NO:73) and FIG. 2C shows the sequence designated O8E (13695; SEQ ID NO:74).

FIG. 3 presents results of microarray expression analysis of the ovarian carcinoma sequence designated O8E.

FIG. 4 presents a partial sequence of a polynucleotide (designated 3g; SEQ ID NO:75) encoding an ovarian carcinoma sequence that is a splice fusion between the human T-cell leukemia virus type I oncoprotein TAX and osteonectin.

FIG. 5 presents the ovarian carcinoma polynucleotide designated 3f (SEQ ID NO:76).

FIG. 6 presents the ovarian carcinoma polynucleotide designated 6b (SEQ ID NO:77).

FIGS. 7A and 7B present the ovarian carcinoma polynucleotides designated 8e (SEQ ID NO:78) and 8h (SEQ ID NO:79).

FIG. 8 presents the ovarian carcinoma polynucleotide designated 12c (SEQ ID NO:80).

FIG. 9 presents the ovarian carcinoma polynucleotide designated 12h (SEQ ID NO:81).

FIG. 10 depicts results of microarray expression analysis of the ovarian carcinoma sequence designated 3f.

FIG. 11 depicts results of microarray expression analysis of the ovarian carcinoma sequence designated 6b.

FIG. 12 depicts results of microarray expression analysis of the ovarian carcinoma sequence designated 8e.

FIG. 13 depicts results of microarray expression analysis of the ovarian carcinoma sequence designated 12c.

FIG. 14 depicts results of microarray expression analysis of the ovarian carcinoma sequence designated 1 2h.

FIGS. 15 A- 15 EEE depict partial sequences of additional polynucleotides encoding representative secreted ovarian carcinoma antigens (SEQ ID NOs:82-310).

DETAILED DESCRIPTION OF THE INVENTION

As noted above, the present invention is generally directed to compositions and methods for the therapy of cancer, such as ovarian cancer. The compositions described herein may include immunogenic polypeptides, nucleic acid sequences encoding such polypeptides, binding agents such as antibodies that bind to a polypeptide and/or immune system cells (e.g., T cells).

Polypeptides of the present invention generally comprise at least an immunogenic portion of an ovarian carcinoma protein or a variant thereof. Certain ovarian carcinoma proteins have been identified using an immunoassay technique, and are referred to herein as ovarian carcinoma antigens. An “ovarian carcinoma antigen” is a protein that is expressed by ovarian tumor cells (preferably human cells) and that reacts detectably (within an immunoassay, such as an ELISA or Western blot) with antisera generated against serum from an immunodeficient animal implanted with a human ovarian tumor. Such ovarian carcinoma antigens are shed or secreted from an ovarian tumor into the sera of the immunodeficient animal. Accordingly, ovarian carcinoma antigens provided herein are generally secreted antigens. Certain nucleic acid sequences of the subject invention generally comprise a DNA or RNA sequence that encodes all or a portion of such a polypeptide, or that is complementary to such a sequence.

The present invention further provides ovarian carcinoma sequences that are identified using techniques to evaluate altered expression within an ovarian tumor. Such sequences may be polynucleotide or protein sequences. Ovarian carcinoma sequences are generally expressed in an ovarian tumor at a level that is at least two fold, and preferably at least five fold, greater than the level of expression in normal ovarian tissue, as determined using a representative assay provided herein. Certain ovarian carcinoma partial polynucleotide sequences are presented in FIGS. 4-9 as well as SEQ ID NOs:75-81. Proteins encoded by genes comprising such polynucleotide sequences (or complements thereof) are also considered ovarian carcinoma proteins.

Antibodies are generally immune system proteins, or antigen-binding fragments thereof, that are capable of binding to a portion of a polypeptide as described above. T cells that may be employed within such compositions are generally T cells (e.g., CD4 + and/or CD8 + ) that are specific for a polypeptide as described above. Certain methods described herein further employ antigen-presenting cells that express an ovarian carcinoma polypeptide as provided herein.

Ovarian Carcinoma Polynucleotides

Any polynucleotide that encodes an ovarian carcinoma protein or a portion or other variant thereof as described herein is encompassed by the present invention. Preferred polynucleotides comprise at least 15 consecutive nucleotides, and preferably at least 30 consecutive nucleotides, that encode a portion of an ovarian carcinoma protein. More preferably, a polynucleotide encodes an immunogenic portion of an ovarian carcinoma protein, such as an ovarian carcinoma antigen. Polynucleotides complementary to any such sequences are also encompassed by the present invention. Polynucleotides may be single-stranded (coding or antisense) or double-stranded, and may be DNA (genomic, cDNA or synthetic) or RNA molecules. Additional coding or non-coding sequences may, but need not, be present within a polynucleotide of the present invention, and a polynucleotide may, but need not, be linked to other molecules and/or support materials.

Polynucleotides may comprise a native sequence (i.e., an endogenous sequence that encodes an ovarian carcinoma protein or a portion thereof) or may comprise a variant of such a sequence. Polynucleotide variants may contain one or more substitutions, additions, deletions and/or insertions such that the immunogenicity of the encoded polypeptide is not diminished, relative to a native ovarian carcinoma protein. The effect on the immunogenicity of the encoded polypeptide may generally be assessed as described herein. Variants preferably exhibit at least about 70% identity, more preferably at least about 80% identity and most preferably at least about 90% identity to a polynucleotide sequence that encodes a native ovarian carcinoma protein or a portion thereof. The percent identity may be readily determined by comparing sequences using computer algorithms well known to those of ordinary skill in the art, such as Megalign, using default parameters. Certain variants are substantially homologous to a native gene, or a portion or complement thereof. Such polynucleotide variants are capable of hybridizing under moderately stringent conditions to a naturally occurring DNA sequence encoding a native ovarian carcinoma sequence (or a complementary sequence). Suitable moderately stringent conditions include prewashing in a solution of 5×SSC, 0.5% SDS, 1.0 mM EDTA (pH 8.0); hybridizing at 50° C.-65° C., 5×SSC, overnight; followed by washing twice at 65° C. for 20 minutes with each of 2×, 0.5× and 0.2×SSC containing 0.1% SDS).

It will be appreciated by those of ordinary skill in the art that, as a result of the degeneracy of the genetic code, there are many nucleotide sequences that encode a polypeptide as described herein. Some of these polynucleotides bear minimal homology to the nucleotide sequence of any native gene. Nonetheless, polynucleotides that vary due to differences in codon usage are specifically contemplated by the present invention.

Polynucleotides may be prepared using any of a variety of techniques. For example, an ovarian carcinoma polynucleotide may be identified, as described in more detail below, by screening a late passage ovarian tumor expression library with antisera generated against sera of immunocompetent mice after injection of such mice with sera from SCID mice implanted with late passage ovarian tumors. Ovarian carcinoma polynucleotides may also be identified using any of a variety of techniques designed to evaluate differential gene expression. Alternatively, polypeptides may be amplified from cDNA prepared from ovarian tumor cells. Such polynucleotides may be amplified via polymerase chain reaction (PCR). For this approach, sequence-specific primers may be designed based on the sequences provided herein, and may be purchased or synthesized.

An amplified portion may be used to isolate a full length gene from a suitable library (e.g., an ovarian carcinoma cDNA library) using well known techniques. Within such techniques, a library (cDNA or genomic) is screened using one or more polynucleotide probes or primers suitable for amplification. Preferably, a library is size-selected to include larger molecules. Random primed libraries may also be preferred for identifying 5′ and upstream regions of genes. Genomic libraries are preferred for obtaining introns and extending 5′ sequences.

For hybridization techniques, a partial sequence may be labeled (e.g., by nick-translation or end-labeling with 32 P) using well known techniques. A bacterial or bacteriophage library is then screened by hybridizing filters containing denatured bacterial colonies (or lawns containing phage plaques) with the labeled probe (see Sambrook et al., Molecular Cloning. A Laboratory Manual , Cold Spring Harbor Laboratories, Cold Spring Harbor, N.Y., 1989). Hybridizing colonies or plaques are selected and expanded, and the DNA is isolated for further analysis. cDNA clones may be analyzed to determine the amount of additional sequence by, for example, PCR using a primer from the partial sequence and a primer from the vector. Restriction maps and partial sequences may be generated to identify one or more overlapping clones. The complete sequence may then be determined using standard techniques, which may involve generating a series of deletion clones. The resulting overlapping sequences are then assembled into a single contiguous sequence. A full length cDNA molecule can be generated by ligating suitable fragments, using well known techniques.

Alternatively, there are numerous amplification techniques for obtaining a full length coding sequence from a partial cDNA sequence. Within such techniques, amplification is generally performed via PCR. Any of a variety of commercially available kits may be used to perform the amplification step. Primers may be designed using, for example, software well known in the art. Primers are preferably 22-30 nucleotides in length, have a GC content of at least 50% and anneal to the target sequence at temperatures of about 68° C. to 72° C. The amplified region may be sequenced as described above, and overlapping sequences assembled into a contiguous sequence.

One such amplification technique is inverse PCR (see Triglia et al., Nuci. Acids Res. 16:8186, 1988), which uses restriction enzymes to generate a fragment in the known region of the gene. The fragment is then circularized by intramolecular ligation and used as a template for PCR with divergent primers derived from the known region. Within an alternative approach, sequences adjacent to a partial sequence may be retrieved by amplification with a primer to a linker sequence and a primer specific to a known region. The amplified sequences are typically subjected to a second round of amplification with the same linker primer and a second primer specific to the known region. A variation on this procedure, which employs two primers that initiate extension in opposite directions from the known sequence, is described in WO 96/38591. Additional techniques include capture PCR (Lagerstrom et al., PCR Methods Applic. 1:111-19, 1991) and walking PCR (Parker et al., Nucl. Acids. Res. 19:3055-60, 1991). Other methods employing amplification may also be employed to obtain a full length cDNA sequence.

In certain instances, it is possible to obtain a full length cDNA sequence by analysis of sequences provided in an expressed sequence tag (EST) database, such as that available from GenBank. Searches for overlapping ESTs may generally be performed using well known programs (e.g., NCBI BLAST searches), and such ESTs may be used to generate a contiguous full length sequence.

Certain nucleic acid sequences of cDNA molecules encoding portions of ovarian carcinoma antigens are provided in FIGS. 1 A‥ 1 S (SEQ ID NOS:1 to 71) and FIGS. 15A to 15 EEE (SEQ ID NOs:82 to 310). These polynucleotides were isolated by serological screening of an ovarian tumor cDNA expression library, using a technique designed to identify secreted tumor antigens. Briefly, a late passage ovarian tumor expression library was prepared from a SCID-derived human ovarian tumor (OV9334) in the vector λ-screen (Novagen). The sera used for screening were obtained by injecting immunocompetent mice with sera from SCID mice implanted with one late passage ovarian tumors. This technique permits the identification of cDNA molecules that encode immunogenic portions of secreted tumor antigens. The polynucleotides recited herein, as well as full length polynucleotides comprising such sequences, other portions of such full length polynucleotides, and sequences complementary to all or a portion of such full length molecules, are specifically encompassed by the present invention. It will be apparent to those of ordinary skill in the art that this technique can also be applied to the identification of antigens that are secreted from other types of tumors.

The sequences provided in FIGS. 1A-1S appear to be novel. For sequences in FIGS. 15 A- 15 EEE, database searches revealed matches having substantial identity.

Other nucleic acid sequences of cDNA molecules encoding portions of ovarian carcinoma proteins are provided in FIGS. 4-9 (SEQ ID NOs:75-81). These sequences were identified by screening a microarray of cDNAs for tumor-associated expression (i.e., expression that is at least five fold greater in an ovarian tumor than in normal ovarian tissue, as determined using a representative assay provided herein). Such screens were performed using a Synteni microarray (Palo Alto, Calif.) according to the manufacturer's instructions (and essentially as described by Schena et al., Proc. Natl. Acad. Sci. USA 93:10614-10619, 1996 and Heller et al., Proc. Natl. Acad. Sci. USA 94:2150-2155, 1997).

Any of a variety of well known techniques may be used to evaluate tumor-associated expression of a cDNA. For example, hybridization techniques using labeled polynucleotide probes may be employed. Alternatively, or in addition, amplification techniques such as real-time PCR may be used (see Gibson et al., Genome Research 6:995-1001, 1996; Heid et al., Genome Research 6:986-994, 1996). Real-time PCR is a technique that evaluates the level of PCR product accumulation during amplification. This technique permits quantitative evaluation of mRNA levels in multiple samples. Briefly, mRNA is extracted from tumor and normal tissue and cDNA is prepared using standard techniques. Real-time PCR may be performed, for example, using a Perkin Elmer/Applied Biosystems (Foster City, Calif.) 7700 Prism instrument. Matching primers and fluorescent probes may be designed for genes of interest using, for example, the primer express program provided by Perkin Elmer/Applied Biosystems (Foster City, Calif.). Optimal concentrations of primers and probes may be initially determined by those of ordinary skill in the art, and control (e.g., β-actin) primers and probes may be obtained commercially from, for example, Perkin Elmer/Applied Biosystems (Foster City, Calif.). To quantitate the amount of specific RNA in a sample, a standard curve is generated alongside using a plasmid containing the gene of interest. Standard curves may be generated using the Ct values determined in the real-time PCR, which are related to the initial cDNA concentration used in the assay. Standard dilutions ranging from 10-10 6 copies of the gene of interest are generally sufficient. In addition, a standard curve is generated for the control sequence. This permits standardization of initial RNA content of a tissue sample to the amount of control for comparison purposes.

Polynucleotide variants may generally be prepared by any method known in the art, including chemical synthesis by, for example, solid phase phosphoramidite chemical synthesis. Modifications in a polynucleotide sequence may also be introduced using standard mutagenesis techniques, such as oligonucleotide-directed site-specific mutagenesis (see Adelman et al., DNA 2:183, 1983). Alternatively, RNA molecules may be generated by in vitro or in vivo transcription of DNA sequences encoding an ovarian carcinoma antigen, or portion thereof, provided that the DNA is incorporated into a vector with a suitable RNA polymerase promoter (such as T7 or SP6). Certain portions may be used to prepare an encoded polypeptide, as described herein. In addition, or alternatively, a portion may be administered to a patient such that the encoded polypeptide is generated in vivo.

A portion of a sequence complementary to a coding sequence (i.e., an antisense polynucleotide) may also be used as a probe or to modulate gene expression. cDNA constructs that can be transcribed into antisense RNA may also be introduced into cells or tissues to facilitate the production of antisense RNA. An antisense polynucleotide may be used, as described herein, to inhibit expression of an ovarian carcinoma protein. Antisense technology can be used to control gene expression through triple-helix formation, which compromises the ability of the double helix to open sufficiently for the binding of polymerases, transcription factors or regulatory molecules (see Gee et al., In Huber and Carr, Molecular and Immunologic Approaches , Futura Publishing Co. (Mt. Kisco, N.Y.; 1994). Alternatively, an antisense molecule may be designed to hybridize with a control region of a gene (e.g., promoter, enhancer or transcription initiation site), and block transcription of the gene; or to block translation by inhibiting binding of a transcript to ribosomes.

Any polynucleotide may be further modified to increase stability in vivo. Possible modifications include, but are not limited to, the addition of flanking sequences at the 5′ and/or 3′ ends; the use of phosphorothioate or 2′ O-methyl rather than phosphodiesterase linkages in the backbone; and/or the inclusion of nontraditional bases such as inosine, queosine and wybutosine, as well as acetyl- methyl-, thio- and other modified forms of adenine, cytidine, guanine, thymine and uridine.

Nucleotide sequences as described herein may be joined to a variety of other nucleotide sequences using established recombinant DNA techniques. For example, a polynucleotide may be cloned into any of a variety of cloning vectors, including plasmids, phagemids, lambda phage derivatives and cosmids. Vectors of particular interest include expression vectors, replication vectors, probe generation vectors and sequencing vectors. In general, a vector will contain an origin of replication functional in at least one organism, convenient restriction endonuclease sites and one or more selectable markers. Other elements will depend upon the desired use, and will be apparent to those of ordinary skill in the art.

Within certain embodiments, polynucleotides may be formulated so as to permit entry into a cell of a mammal, and expression therein. Such formulations are particularly useful for therapeutic purposes, as described below. Those of ordinary skill in the art will appreciate that there are many ways to achieve expression of a polynucleotide in a target cell, and any suitable method may be employed. For example, a polynucleotide may be incorporated into a viral vector such as, but not limited to, adenovirus, adeno-associated virus, retrovirus, or vaccinia or other pox virus (e.g, avian pox virus). Techniques for incorporating DNA into such vectors are well known to those of ordinary skill in the art. A retroviral vector may additionally transfer or incorporate a gene for a selectable marker (to aid in the identification or selection of transduced cells) and/or a targeting moiety, such as a gene that encodes a ligand for a receptor on a specific target cell, to render the vector target specific. Targeting may also be accomplished using an antibody, by methods known to those of ordinary skill in the art.

Other formulations for therapeutic purposes include colloidal dispersion systems, such as macromolecule complexes, nanocapsules, microspheres, beads, and lipid-based systems including oil-in-water emulsions, micelles, mixed micelles, and liposomes. A preferred colloidal system for use as a delivery vehicle in vitro and in vivo is a liposome (i.e., an artificial membrane vesicle). The preparation and use of such systems is well known in the art.

Ovarian Carcinoma Polypeptides

Within the context of the present invention, polypeptides may comprise at least an immunogenic portion of an ovarian carcinoma protein or a variant thereof, as described herein. As noted above, certain ovarian carcinoma proteins are ovarian carcinoma antigens that are expressed by ovarian tumor cells and react detectably within an immunoassay (such as an ELISA) with antisera generated against serum from an immunodeficient animal implanted with an ovarian tumor. Other ovarian carcinoma proteins are encoded by ovarian carcinoma polynucleotides recited herein. Polypeptides as described herein may be of any length. Additional sequences derived from the native protein and/or heterologous sequences may be present, and such sequences may (but need not) possess further immunogenic or antigenic properties.

An “immunogenic portion,” as used herein is a portion of an antigen that is recognized (i.e., specifically bound) by a B-cell and/or T-cell surface antigen receptor. Such immunogenic portions generally comprise at least 5 amino acid residues, more preferably at least 10, and still more preferably at least 20 amino acid residues of an ovarian carcinoma protein or a variant thereof. Preferred immunogenic portions are encoded by cDNA molecules isolated as described herein. Further immunogenic portions may generally be identified using well known techniques, such as those summarized in Paul, Fundamental Immunology, 3rd ed., 243-247 (Raven Press, 1993) and references cited therein. Such techniques include screening polypeptides for the ability to react with ovarian carcinoma protein-specific antibodies, antisera and/or T-cell lines or clones. As used herein, antisera and antibodies are “ovarian carcinoma protein-specific” if they specifically bind to an ovarian carcinoma protein (i.e., they react with the ovarian carcinoma protein in an ELISA or other immunoassay, and do not react detectably with unrelated proteins). Such antisera, antibodies and T cells may be prepared as described herein, and using well known techniques. An immunogenic portion of a native ovarian carcinoma protein is a portion that reacts with such antisera, antibodies and/or T-cells at a level that is not substantially less than the reactivity of the full length polypeptide (e.g., in an ELISA and/or T-cell reactivity assay). Such immunogenic portions may react within such assays at a level that is similar to or greater than the reactivity of the full length protein. Such screens may generally be performed using methods well known to those of ordinary skill in the art, such as those described in Harlow and Lane, Antibodies: A Laboratory Manual , Cold Spring Harbor Laboratory, 1988. For example, a polypeptide may be immobilized on a solid support and contacted with patient sera to allow binding of antibodies within the sera to the immobilized polypeptide. Unbound sera may then be removed and bound antibodies detected using, for example, 125 I-labeled Protein A.

As noted above, a composition may comprise a variant of a native ovarian carcinoma protein. A polypeptide “variant,” as used herein, is a polypeptide that differs from a native ovarian carcinoma protein in one or more substitutions, deletions, additions and/or insertions, such that the immunogenicity of the polypeptide is not substantially diminished. In other words, the ability of a variant to react with ovarian carcinoma protein-specific antisera may be enhanced or unchanged, relative to the native ovarian carcinoma protein, or may be diminished by less than 50%, and preferably less than 20%, relative to the native ovarian carcinoma protein. Such variants may generally be identified by modifying one of the above polypeptide sequences and evaluating the reactivity of the modified polypeptide with ovarian carcinoma protein-specific antibodies or antisera as described herein.

Preferably, a variant contains conservative substitutions. A “conservative substitution” is one in which an amino acid is substituted for another amino acid that has similar properties, such that one skilled in the art of peptide chemistry would expect the secondary structure and hydropathic nature of the polypeptide to be substantially unchanged. Amino acid substitutions may generally be made on the basis of similarity in polarity, charge, solubility, hydrophobicity, hydrophilicity and/or the amphipathic nature of the residues. For example, negatively charged amino acids include aspartic acid and glutamic acid; positively charged amino acids include lysine and arginine; and amino acids with uncharged polar head groups having similar hydrophilicity values include leucine, isoleucine and valine; glycine and alanine; asparagine and glutamine; and serine, threonine, phenylalanine and tyrosine. Other groups of amino acids that may represent conservative changes include: (1) ala, pro, gly, glu, asp, gln, asn, ser, thr; (2) cys, ser, tyr, thr; (3) val, ile, leu, met, ala, phe; (4) lys, arg, his; and (5) phe, tyr, trp, his. A variant may also, or alternatively, contain nonconservative changes. Variants may also (or alternatively) be modified by, for example, the deletion or addition of amino acids that have minimal influence on the immunogenicity, secondary structure and hydropathic nature of the polypeptide.

As noted above, polypeptides may comprise a signal (or leader) sequence at the N-terminal end of the protein which co-translationally or post-translationally directs transfer of the protein. The polypeptide may also be conjugated to a linker or other sequence for ease of synthesis, purification or identification of the polypeptide (e.g., poly-His), or to enhance binding of the polypeptide to a solid support. For example, a polypeptide may be conjugated to an immunoglobulin Fc region.

Polypeptides may be prepared using any of a variety of well known techniques. Recombinant polypeptides encoded by DNA sequences as described above may be readily prepared from the DNA sequences using any of a variety of expression vectors known to those of ordinary skill in the art. Expression may be achieved in any appropriate host cell that has been transformed or transfected with an expression vector containing a DNA molecule that encodes a recombinant polypeptide. Suitable host cells include prokaryotes, yeast and higher eukaryotic cells. Preferably, the host cells employed are E. coli , yeast or a mammalian cell line such as COS or CHO. Supernatants from suitable host/vector systems which secrete recombinant protein or polypeptide into culture media may be first concentrated using a commercially available filter. Following concentration, the concentrate may be applied to a suitable purification matrix such as an affinity matrix or an ion exchange resin. Finally, one or more reverse phase HPLC steps can be employed to further purify a recombinant polypeptide.

Portions and other variants having fewer than about 100 amino acids, and generally fewer than about 50 amino acids, may also be generated by synthetic means, using techniques well known to those of ordinary skill in the art. For example, such polypeptides may be synthesized using any of the commercially available solid-phase techniques, such as the Merrifield solid-phase synthesis method, where amino acids are sequentially added to a growing amino acid chain. See Merrifield, J. Am. Chem. Soc. 85:2149-2146, 1963. Equipment for automated synthesis of polypeptides is Lcommercially available from suppliers such as Applied BioSystems, Inc. (Foster City, Calif.), and may be operated according to the manufacturer's instructions.

Within certain specific embodiments, a polypeptide may be a fusion protein that comprises multiple polypeptides as described herein, or that comprises one polypeptide as described herein and a known tumor antigen, such as an ovarian carcinoma protein or a variant of such a protein. Fusion proteins may generally be prepared using standard techniques. For example, a fusion protein may be prepared recombinantly. Briefly, DNA sequences encoding the polypeptide components may be assembled separately, and ligated into an appropriate expression vector. The 3′ end of the DNA sequence encoding one polypeptide component is ligated, with or without a peptide linker, to the 5′ end of a DNA sequence encoding the second polypeptide component so that the reading frames of the sequences are in phase. This permits translation into a single fusion protein that retains the biological activity of both component polypeptides.

A peptide linker sequence may be employed to separate the first and the second polypeptide components by a distance sufficient to ensure that each polypeptide folds into its secondary and tertiary structures. Such a peptide linker sequence is incorporated into the fusion protein using standard techniques well known in the art. Suitable peptide linker sequences may be chosen based on the following factors: (1) their ability to adopt a flexible extended conformation; (2) their inability to adopt a secondary structure that could interact with functional epitopes on the first and second polypeptides; and (3) the lack of hydrophobic or charged residues that might react with the polypeptide functional epitopes. Preferred peptide linker sequences contain Gly, Asn and Ser residues. Other near neutral amino acids, such as Thr and Ala may also be used in the linker sequence. Amino acid sequences which may be usefully employed as linkers include those disclosed in Maratea et al., Gene 40:39-46, 1985; Murphy et al., Proc. Natl. Acad Sci . USA 83:8258-8262, 1986; U.S. Pat. Nos. 4,935,233 and 4,751,180. The linker sequence may generally be from 1 to about 50 amino acids in length. Linker sequences are not required when the first and second polypeptides have non-essential N-terminal amino acid regions that can be used to separate the functional domains and prevent steric interference.

The ligated DNA sequences are operably linked to suitable transcriptional or translational regulatory elements. The regulatory elements responsible for expression of DNA are located only 5′ to the DNA sequence encoding the first polypeptides. Similarly, stop codons required to end translation and transcription termination signals are only present 3′ to the DNA sequence encoding the second polypeptide.

Fusion proteins are also provided that comprise a polypeptide of the present invention together with an unrelated immunogenic protein. Preferably the immunogenic protein is capable of eliciting a recall response. Examples of such proteins include tetanus, tuberculosis and hepatitis proteins (see, for example, Stoute et al. New Engl. J. Med., 336:86-91, 1997).

In general, polypeptides (including fusion proteins) and polynucleotides as described herein are isolated. An “isolated” polypeptide or polynucleotide is one that is removed from its original environment. For example, a naturally-occurring protein is isolated if it is separated from some or all of the coexisting materials in the natural system. Preferably, such polypeptides are at least about 90% pure, more preferably at least about 95% pure and most preferably at least about 99% pure. A polynucleotide is considered to be isolated if, for example, it is cloned into a vector that is not a part of the natural environment.

Binding Agents

The present invention further provides agents, such as antibodies and antigen-binding fragments thereof, that specifically bind to an ovarian carcinoma protein. As used herein, an antibody, or antigen-binding fragment thereof, is said to “specifically bind” to an ovarian carcinoma protein if it reacts at a detectable level (within, for example, an ELISA) with an ovarian carcinoma protein, and does not react detectably with unrelated proteins under similar conditions. As used herein, “binding” refers to a noncovalent association between two separate molecules such that a “complex” is formed. The ability to bind may be evaluated by, for example, determining a binding constant for the formation of the complex. The binding constant is the value obtained when the concentration of the complex is divided by the product of the component concentrations. In general, two compounds are said to “bind,” in the context of the present invention, when the binding constant for complex formation exceeds about 10 3 L/mol. The binding constant maybe determined using methods well known in the art.

Any agent that satisfies the above requirements may be a binding agent. For example, a binding agent may be a ribosome, with or without a peptide component, and RNA molecule or a polypeptide. In a preferred embodiment, a binding agent is an antibody or an antigen-binding fragment thereof. Antibodies may be prepared by any of a variety of techniques known to those of ordinary skill in the art. See, e.g., Harlow and Lane, Antibodies: A Laboratory Manual , Cold Spring Harbor Laboratory, 1988. In general, antibodies can be produced by cell culture techniques, including the generation of monoclonal antibodies as described herein, or via transfection of antibody genes into suitable bacterial or mammalian cell hosts, in order to allow for the production of recombinant antibodies. In one technique, an immunogen comprising the polypeptide is initially injected into any of a wide variety of manuals (e.g., mice, rats, rabbits, sheep or goats). In this step, the polypeptides of this invention may serve as the immunogen without modification. Alternatively, particularly for relatively short polypeptides, a superior immune response may be elicited if the polypeptide is joined to a carrier protein, such as bovine serum albumin or keyhole limpet hemocyanin. The immunogen is injected into the animal host, preferably according to a predetermined schedule incorporating one or more booster immunizations, and the animals are bled periodically. Polyclonal antibodies specific for the polypeptide may then be purified from such antisera by, for example, affinity chromatography using the polypeptide coupled to a suitable solid support.

Monoclonal antibodies specific for an antigenic polypeptide of interest may be prepared, for example, using the technique of Kohler and Milstein, Eur. J. Immunol. 6:511-519, 1976, and improvements thereto. Briefly, these methods involve the preparation of immortal cell lines capable of producing antibodies having the desired specificity (i.e., reactivity with the polypeptide of interest). Such cell lines may be produced, for example, from spleen cells obtained from an animal immunized as described above. The spleen cells are then immortalized by, for example, fusion with a myeloma cell fusion partner, preferably one that is syngeneic with the immunized animal. A variety of fusion techniques may be employed. For example, the spleen cells and myeloma cells may be combined with a nonionic detergent for a few minutes and then plated at low density on a selective medium that supports the growth of hybrid cells, but not myeloma cells. A preferred selection technique uses HAT (hypoxanthine, aminopterin, thymidine) selection. After a sufficient time, usually about 1 to 2 weeks, colonies of hybrids are observed. Single colonies are selected and their culture supernatants tested for binding activity against the polypeptide. Hybridomas having high reactivity and specificity are preferred.

Monoclonal antibodies may be isolated from the supernatants of growing hybridoma colonies. In addition, various techniques may be employed to enhance the yield, such as injection of the hybridoma cell line into the peritoneal cavity of a suitable vertebrate host, such as a mouse. Monoclonal antibodies may then be harvested from the ascites fluid or the blood. Contaminants may be removed from the antibodies by conventional techniques, such as chromatography, gel filtration, precipitation, and extraction. The polypeptides of this invention may be used in the purification process in, for example, an affinity chromatography step.

Within certain embodiments, the use of antigen-binding fragments of antibodies may be preferred. Such fragments include Fab fragments, which may be prepared using standard techniques. Briefly, immunoglobulins may be purified from rabbit serum by affinity chromatography on Protein A bead columns (Harlow and Lane, Antibodies. A Laboratory Manual , Cold Spring Harbor Laboratory, 1988) and digested by papain to yield Fab and Fc fragments. The Fab and Fc fragments may be separated by affinity chromatography on protein A bead columns.

Monoclonal antibodies of the present invention may be coupled to one or more therapeutic agents. Suitable agents in this regard include radionuclides, differentiation inducers, drugs, toxins, and derivatives thereof. Preferred radionuclides include 90 Y, 123 I, 125 I, 131 I, 186 Re, 188 Re, 211 At, and 212 Bi. Preferred drugs include methotrexate, and pyrimidine and purine analogs. Preferred differentiation inducers include phorbol esters and butyric acid. Preferred toxins include ricin, abrin, diptheria toxin, cholera toxin, gelonin, Pseudomonas exotoxin, Shigella toxin, and pokeweed antiviral protein.

A therapeutic agent may be coupled (e.g., covalently bonded) to a suitable monoclonal antibody either directly or indirectly (e.g., via a linker group). A direct reaction between an agent and an antibody is possible when each possesses a substituent capable of reacting with the other. For example, a nucleophilic group, such as an amino or sulfhydryl group, on one may be capable of reacting with a carbonyl-containing group, such as an anhydride or an acid halide, or with an alkyl group containing a good leaving group (e.g., a halide) on the other.

Alternatively, it may be desirable to couple a therapeutic agent and an antibody via a linker group. A linker group can function as a spacer to distance an antibody from an agent in order to avoid interference with binding capabilities. A linker group can also serve to increase the chemical reactivity of a substituent on an agent or an antibody, and thus increase the coupling efficiency. An increase in chemical reactivity may also facilitate the use of agents, or functional groups on agents, which otherwise would not be possible.

It will be evident to those skilled in the art that a variety of bifunctional or polyfunctional reagents, both homo- and hetero-functional (such as those described in the catalog of the Pierce Chemical Co., Rockford, Ill.), may be employed as the linker group. Coupling may be effected, for example, through amino groups, carboxyl groups, sulfhydryl groups or oxidized carbohydrate residues. There are numerous references describing such methodology, e.g., U.S. Pat. No. 4,671,958, to Rodwell et al.

Where a therapeutic agent is more potent when free from the antibody portion of the immunoconjugates of the present invention, it may be desirable to use a linker group which is cleavable during or upon internalization into a cell. A number of different cleavable linker groups have been described. The mechanisms for the intracellular release of an agent from these linker groups include cleavage by reduction of a disulfide bond (e.g., U.S. Pat. No. 4,489,710, to Spitler), by irradiation of a photolabile bond (e.g., U.S. Pat. No. 4,625,014, to Senter et al.). by hydrolysis of derivatized amino acid side chains (e.g., U.S. Pat. No. 4,638,045, to Kohn et al.), by serum complement-mediated hydrolysis (e.g., U.S. Pat. No. 4,671,958, to Rodwell et al.), and acid-catalyzed hydrolysis (e.g., U.S. Pat. No. 4,569,789, to Blattler et al.).

It may be desirable to couple more than one agent to an antibody. In one embodiment, multiple molecules of an agent are coupled to one antibody molecule. In another embodiment, more than one type of agent may be coupled to one antibody. Regardless of the particular embodiment, immunoconjugates with more than one agent may be prepared in a variety of ways. For example, more than one agent may be coupled directly to an antibody molecule, or linkers which provide multiple sites for attachment can be used. Alternatively, a carrier can be used.

A carrier may bear the agents in a variety of ways, including covalent bonding either directly or via a linker group. Suitable carriers include proteins such as albumins (e.g, U.S. Pat. No. 4,507,234, to Kato et al.), peptides and polysaccharides such as aminodextran (e.g., U.S. Pat. No. 4,699,784, to Shih et al.). A carrier may also bear an agent by noncovalent bonding or by encapsulation, such as within a liposome vesicle (e.g., U.S. Pat. Nos. 4,429,008 and 4,873,088). Carriers specific for radionuclide agents include radiohalogenated small molecules and chelating compounds. For example, U.S. Pat. No. 4,735,792 discloses representative radiohalogenated small molecules and their synthesis. A radionuclide chelate may be formed from chelating compounds that include those containing nitrogen and sulfur atoms as the donor atoms for binding the metal, or metal oxide, radionuclide. For example, U.S. Pat. No. 4,673,562, to Davison et al. discloses representative chelating compounds and their synthesis.

A variety of routes of administration for the antibodies and immunoconjugates may be used. Typically, administration will be intravenous, intramuscular, subcutaneous or in the bed of a resected tumor. It will be evident that the precise dose of the antibody/immunoconjugate will vary depending upon the antibody used, the antigen density on the tumor, and the rate of clearance of the antibody.

Also provided herein are anti-idiotypic antibodies that mimic an immunogenic portion of an ovarian carcinoma protein. Such antibodies may be raised against an antibody, or antigen-binding fragment thereof, that specifically binds to an immunogenic portion of an ovarian carcinoma protein, using well known techniques. Anti-idiotypic antibodies that mimic an immunogenic portion of an ovarian carcinoma protein are those antibodies that bind to an antibody, or antigen-binding fragment thereof, that specifically binds to an immunogenic portion of an ovarian carcinoma protein, as described herein.

T Cells

Immunotherapeutic compositions may also, or alternatively, comprise T cells specific for an ovarian carcinoma protein. Such cells may generally be prepared in vitro or ex vivo, using standard procedures. For example, T cells may be present within (or isolated from) bone marrow, peripheral blood or a fraction of bone marrow or peripheral blood of a mammal, such as a patient, using a commercially available cell separation system, such as the CEPRATE™ system, available from CellPro Inc., Bothell Wash. (see also U.S. Pat. Nos. 5,240,856; 5,215,926; WO 89/06280; WO 91/16116 and WO 92/07243). Alternatively, T cells may be derived from related or unrelated humans, non-human animals, cell lines or cultures.

T cells may be stimulated with an ovarian carcinoma polypeptide, polynucleotide encoding an ovarian carcinoma polypeptide and/or an antigen presenting cell (APC) that expresses such a polypeptide. Such stimulation is performed under conditions and for a time sufficient to permit the generation of T cells that are specific for the polypeptide. Preferably, an ovarian carcinoma polypeptide or polynucleotide is present within a delivery vehicle, such as a microsphere, to facilitate the generation of specific T cells.

T cells are considered to be specific for an ovarian carcinoma polypeptide if the T cells kill target cells coated with an ovarian carcinoma polypeptide or expressing a gene encoding such a polypeptide. T cell specificity may be evaluated using any of a variety of standard techniques. For example, within a chromium release assay or proliferation assay, a stimulation index of more than two fold increase in lysis and/or proliferation, compared to negative controls, indicates T cell specificity. Such assays may be performed, for example, as described in Chen et al., Cancer Res. 54:1065-1070, 1994. Alternatively, detection of the proliferation of T cells may be accomplished by a variety of known techniques. For example, T cell proliferation can be detected by measuring an increased rate of DNA synthesis (e.g., by pulse-labeling cultures of T cells with tritiated thymidine and measuring the amount of tritiated thymidine incorporated into DNA). Contact with an ovarian carcinoma polypeptide (200 ng/ml-100 μg/ml, preferably 100 ng/ml-25 μg/ml) for 3-7 days should result in at least a two fold increase in proliferation of the T cells and/or contact as described above for 2-3 hours should result in activation of the T cells, as measured using standard cytokine assays in which a two fold increase in the level of cytokine release (e.g., TNF or IFN-γ) is indicative of T cell activation (see Coligan et al., Current Protocols in Immunology, vol. 1, Wiley Interscience (Greene 1998). T cells that have been activated in response to an ovarian carcinoma polypeptide, polynucleotide or ovarian carcinoma polypeptide-expressing APC may be CD4 − and/or CD8 + . Ovarian carcinoma polypeptide-specific T cells may be expanded using standard techniques. Within preferred embodiments, the T cells are derived from a patient or a related or unrelated donor and are administered to the patient following stimulation and expansion.

For therapeutic purposes, CD4 + or CD8 + T cells that proliferate in response to an ovarian carcinoma polypeptide, polynucleotide or APC can be expanded in number either in vitro or in vivo. Proliferation of such T cells in vitro may be accomplished in a variety of ways. For example, the T cells can be re-exposed to an ovarian carcinoma polypeptide, with or without the addition of T cell growth factors, such as interleukin-2, and/or stimulator cells that synthesize an ovarian carcinoma polypeptide. Alternatively, one or more T cells that proliferate in the presence of an ovarian carcinoma polypeptide can be expanded in number by cloning. Methods for cloning cells are well known in the art, and include limiting dilution.

Pharmaceutical Compositions and Vaccines

Within certain aspects, polypeptides, polynucleotides, binding agents and/or immune system cells as described herein may be incorporated into pharmaceutical compositions or vaccines. Pharmaceutical compositions comprise one or more such compounds or cells and a physiologically acceptable carrier. Vaccines may comprise one or more such compounds or cells and a non-specific immune response enhancer. A non-specific immune response enhancer may be any substance that enhances an immune response to an exogenous antigen. Examples of non-specific immune response enhancers include adjuvants, biodegradable microspheres (e.g., polylactic galactide) and liposomes (into which the compound is incorporated). Pharmaceutical compositions and vaccines within the scope of the present invention may also contain other compounds, which may be biologically active or inactive. For example, one or more immunogenic portions of other tumor antigens may be present, either incorporated into a fusion polypeptide or as a separate compound within the composition or vaccine.

A pharmaceutical composition or vaccine may contain DNA encoding one or more of the polypeptides as described above, such that the polypeptide is generated in situ. As noted above, the DNA may be present within any of a variety of delivery systems known to those of ordinary skill in the art, including nucleic acid expression systems, bacteria and viral expression systems. Appropriate nucleic acid expression systems contain the necessary DNA sequences for expression in the patient (such as a suitable promoter and terminating signal). Bacterial delivery systems involve the administration of a bacterium (such as Bacillus-Calmette-Guerrin) that expresses an immunogenic portion of the polypeptide on its cell surface. In a preferred embodiment, the DNA may be introduced using a viral expression system (e.g., vaccinia or other pox virus, retrovirus, or adenovirus), which may involve the use of a non-pathogenic (defective), replication competent virus. Suitable systems are disclosed, for example, in Fisher-Hoch et al., PNAS 86:317-321, 1989; Flexner et al., Ann. N.Y. Acad Sci. 569:86-103, 1989; Flexner et al., Vaccine 8:17-21, 1990; U.S. Pat. Nos. 4,603,112, 4,769,330, and 5,017,487; WO 89/01973; U.S. Pat. No.4,777,127; GB 2,200,651; EP 0,345,242; WO 91/02805; Berkner, Biotechniques 6:616-627, 1988; Rosenfeld et al., Science 252:431-434, 1991; Kolls et al., PNAS 91:215-219, 1994; Kass-Eisler et al., PNAS 90:11498-11502, 1993; Guzman et al., Circulation 88:2838-2848, 1993; and Guzman et al., Cir. Res. 73:1202-1207, 1993. Techniques for incorporating DNA into such expression systems are well known to those of ordinary skill in the art. The DNA may also be “naked,” as described, for example, in Ulmer et al., Science 259:1745-1749, 1993 and reviewed by Cohen, Science 259:1691-1692, 1993. The uptake of naked DNA may be increased by coating the DNA onto biodegradable beads, which are efficiently transported into the cells.

While any suitable carrier known to those of ordinary skill in the art may be employed in the pharmaceutical compositions of this invention, the type of carrier will vary depending on the mode of administration. Compositions of the present invention may be formulated for any appropriate manner of administration, including for example, topical, oral, nasal, intravenous, intracranial, intraperitoneal, subcutaneous or intramuscular administration. For parenteral administration, such as subcutaneous injection, the carrier preferably comprises water, saline, alcohol, a fat, a wax or a buffer. For oral administration, any of the above carriers or a solid carrier, such as mannitol, lactose, starch, magnesium stearate, sodium saccharine, talcum, cellulose, glucose, sucrose, and magnesium carbonate, may be employed. Biodegradable microspheres (e.g., polylactate polyglycolate) may also be employed as carriers for the pharmaceutical compositions of this invention. Suitable biodegradable microspheres are disclosed, for example, in U.S. Pat. Nos. 4,897,268 and 5,075,109.

Such compositions may also comprise buffers (e.g., neutral buffered saline or phosphate buffered saline), carbohydrates (e.g., glucose, mannose, sucrose or dextrans), mannitol, proteins, polypeptides or amino acids such as glycine, antioxidants, chelating agents such as EDTA or glutathione, adjuvants (e.g., aluminum hydroxide) and/or preservatives. Alternatively, compositions of the present invention may be formulated as a lyophilizate. Compounds may also be encapsulated within liposomes using well known technology.

Any of a variety of non-specific immune response enhancers may be employed in the vaccines of this invention. For example, an adjuvant may be included. Most adjuvants contain a substance designed to protect the antigen from rapid catabolism, such as aluminum hydroxide or mineral oil, and a stimulator of immune responses, such as lipid A, Bortadella pertussis or Mycobacterium tuberculosis derived proteins. Suitable adjuvants are commercially available as, for example, Freund's Incomplete Adjuvant and Complete Adjuvant (Difco Laboratories, Detroit, Mich.), Merck Adjuvant 65 (Merck and Company, Inc., Rahway, N.J.), alum, biodegradable microspheres, monophosphoryl lipid A and quil A. Cytokines, such as GM-CSF or interleukin-2, -7, or -12, may also be used as adjuvants.

The compositions described herein may be administered as part of a sustained release formulation (i.e., a formulation such as a capsule or sponge that effects a slow release of compound following administration). Such formulations may generally be prepared using well known technology and administered by, for example, oral, rectal or subcutaneous implantation, or by implantation at the desired target site. Sustained-release formulations may contain a polypeptide, polynucleotide or antibody dispersed in a carrier matrix and/or contained within a reservoir surrounded by a rate controlling membrane. Carriers for use within such formulations are biocompatible, and may also be biodegradable; preferably the formulation provides a relatively constant level of active component release. The amount of active compound contained within a sustained release formulation depends upon the site of implantation, the rate and expected duration of release and the nature of the condition to be treated or prevented.

Cancer Therapy

In further aspects of the present invention, the compositions described herein may be used for immunotherapy of cancer, such as ovarian cancer. Within such methods, pharmaceutical compositions and vaccines are typically administered to a patient. As used herein, a “patient” refers to any warm-blooded animal, preferably a human. A patient may or may not be afflicted with cancer. Accordingly, the above pharmaceutical compositions and vaccines may be used to prevent the development of a cancer or to treat a patient afflicted with a cancer. Within certain preferred embodiments, a patient is afflicted with ovarian cancer. Such cancer may be diagnosed using criteria generally accepted in the art, including the presence of a malignant tumor. Pharmaceutical compositions and vaccines may be administered either prior to or following surgical removal of primary tumors and/or treatment such as administration of radiotherapy or conventional chemotherapeutic drugs.

Within certain embodiments, immunotherapy may be active immunotherapy, in which treatment relies on the in vivo stimulation of the endogenous host immune system to react against tumors with the administration of immuno response-modifying agents (such as tumor vaccines, bacterial adjuvants and/or cytokines).

Within other embodiments, immunotherapy may be passive immunotherapy, in which treatment involves the delivery of agents with established tumor-immune reactivity (such as effector cells or antibodies) that can directly or indirectly mediate antitumor effects and does not necessarily depend on an intact host immune system. Examples of effector cells include T lymphocytes (such as CD8 − cytotoxic T lymphocytes and CD4 + T-helper tumor-infiltrating lymphocytes), killer cells (such as Natural Killer cells and lymphokine-activated killer cells), B cells and antigen-presenting cells (such as dendritic cells and macrophages) expressing a polypeptide provided herein. T cell receptors and antibody receptors specific for the polypeptides recited herein may be cloned, expressed and transferred into other vectors or effector cells for adoptive immunotherapy. The polypeptides provided herein may also be used to generate antibodies or anti-idiotypic antibodies (as described above and in U.S. Pat. No. 4,918,164) for passive immunotherapy.

Effector cells may generally be obtained in sufficient quantities for adoptive immunotherapy by growth in vitro, as described herein. Culture conditions for expanding single antigen-specific effector cells to several billion in number with retention of antigen recognition in vivo are well known in the art. Such in vitro culture conditions typically use intermittent stimulation with antigen, often in the presence of cytokines (such as IL-2) and non-dividing feeder cells. As noted above, immunoreactive polypeptides as provided herein may be used to rapidly expand antigen-specific T cell cultures in order to generate a sufficient number of cells for immunotherapy. In particular, antigen-presenting cells, such as dendritic, macrophage or B cells, may be pulsed with immunoreactive polypeptides or transfected with one or more polynucleotides using standard techniques well known in the art. For example, antigen-presenting cells can be transfected with a polynucleotide having a promoter appropriate for increasing expression in a recombinant virus or other expression system. Cultured effector cells for use in therapy must be able to grow and distribute widely, and to survive long term in vivo. Studies have shown that cultured effector cells can be induced to grow in vivo and to survive long term in substantial numbers by repeated stimulation with antigen supplemented with IL-2 (see, for example, Cheever et al., Immunological Reviews 157:177, 1997).

The polypeptides provided herein may also be used to generate and/or isolate tumor-reactive T cells, which can then be administered to a patient. In one such technique, antigen-specific T cell lines may be generated by in vivo immunization with short peptides corresponding to immunogenic portions of the disclosed polypeptides. The resulting antigen-specific CD8 + CTL clones may be isolated from the patient, expanded using standard tissue culture techniques and returned to the patient.

Polypeptides may also be used for ex vivo treatment of a cancer, such as ovanan cancer. For example, cells of the immune system, such as T cells, may be isolated from the peripheral blood of a patient, using a commercially available cell separation system, such as CellPro Incorporated's (Bothell, Wash.) CEPRATE™ system (see U.S. Pat. Nos. 5,240,856; 5,215,926; WO 89/06280; WO 91/16116 and WO 92/07243). The separated cells are stimulated with one or more of the immunoreactive polypeptides contained within a delivery vehicle, such as a microsphere, to provide antigen-specific T cells. The population of tumor antigen-specific T cells is them expanded using standard techniques and the cells may be administered back to the patient as described, for example, by Chang et al., Crit. Rev. Oncol. Hematol. 22:213, 1996.

Within another embodiment, syngeneic or autologous dendritic-cells may be pulsed with peptides corresponding to at least an immunogenic portion of a polypeptide disclosed herein. The resulting antigen-specific dendritic cells may either be transferred into a patient or employed to stimulate T cells to provide antigen-specific T cells which may, in turn, be administered to a patient. The use of peptide-pulsed dendritic cells to generate antigen-specific T cells and the subsequent use of such antigen-specific T cells to eradicate tumors in a murine model has been demonstrated by Cheever et al., Immunological Reviews 157:177, 1997.

Alternatively, a vector expressing a polypeptide recited herein may be introduced into stem cells taken from a patient and clonally propagated in vitro for autologous transplant back into the same patient.

Routes and frequency of administration, as well as dosage, will vary from individual to individual, and may be readily established using standard techniques. In general, the pharmaceutical compositions and vaccines may be administered by injection (e.g., intracutaneous, intramuscular, intravenous or subcutaneous), intranasally (e.g., by aspiration), orally or in the bed of a resected tumor. Preferably, between 1 and 10 doses may be administered over a 52 week period. Preferably, 6 doses are administered, at intervals of 1 month, and booster vaccinations may be given periodically thereafter. Alternate protocols may be appropriate for individual patients. A suitable dose is an amount of a compound that, when administered as described above, is capable of promoting an anti-tumor immune response, and is at least 10-50% above the basal (i.e., untreated) level. Such response can be monitored by measuring the anti-tumor antibodies in a patient or by vaccine-dependent generation of cytolytic effector cells capable of killing the patient's tumor cells in vitro. Such vaccines should also be capable of causing an immune response that leads to an improved clinical outcome (e.g., more frequent remissions, complete or partial or longer disease-free survival) in vaccinated patients as compared to non-vaccinated patients. In general, for pharmaceutical compositions and vaccines comprising one or more polypeptides, the amount of each polypeptide present in a dose ranges from about 100 μg to 5 mg per kg of host. Suitable dose sizes will vary with the size of the patient, but will typically range from about 0.1 mL to about 5 mL.

In general, an appropriate dosage and treatment regimen provides the active compound(s) in an amount sufficient to provide therapeutic and/or prophylactic benefit. Such a response can be monitored by establishing an improved clinical outcome (e.g., more frequent remissions, complete or partial, or longer disease-free survival) in treated patients as compared to non-treated patients. Increases in preexisting immune responses to an ovarian carcinoma antigen generally correlate with an improved clinical outcome. Such immune responses may generally be evaluated using standard proliferation, cytotoxicity or cytokine assays, which may be performed using samples obtained from a patient before and after treatment.

Screens for Identifying Secreted Ovarian Carcinoma Antigens

The present invention provides methods for identifying secreted tumor antigens. Within such methods, tumors are implanted into immunodeficient animals such as SCID mice and maintained for a time sufficient to permit secretion of tumor antigens into serum. In general, tumors may be implanted subcutaneously or within the gonadal fat pad of an immunodeficient animal and maintained for 1-9 months, preferably 1-4 months. Implantation may generally be performed as described in WO 97/18300. The serum containing secreted antigens is then used to prepare antisera in immunocompetent mice, using standard techniques and as described herein. Briefly, 50-100 μL of sera (pooled from three sets of immunodeficient mice, each set bearing a different SCID-derived human ovarian tumor) may be mixed 1:1 (vol:vol) with an appropriate adjuvant, such as RIBI-MPL or MPL+TDM (Sigma Chemical Co., St. Louis, Mo.) and injected intraperitoneally into syngeneic immunocompetent animals at monthly intervals for a total of 5 months. Antisera from animals immunized in such a manner may be obtained by drawing blood after the third, fourth and fifth immunizations. The resulting antiserum is generally pre-cleared of E. coli and phage antigens and used (generally following dilution, such as 1:200) in a serological expression screen.

The library is typically an expression library containing cDNAs from one or more tumors of the type that was implanted into SCID mice. This expression library may be prepared in any suitable vector, such as λ-screen (Novagen). cDNAs that encode a polypeptide that reacts with the antiserum may be identified using standard techniques, and sequenced. Such cDNA molecules may be further characterized to evaluate expression in tumor and normal tissue, and to evaluate antigen secretion in patients.

The methods provided herein have advantages over other methods for tumor antigen discovery. In particular, all antigens identified by such methods should be secreted or released through necrosis of the tumor cells. Such antigens may be present on the surface of tumor cells for an amount of time sufficient to permit targeting and killing by the immune system, following vaccination.

The following Examples are offered by way of illustration and not by way of limitation.

EXAMPLES

Example 1

Identification of Representative Ovarian Carcinoma Antigen cDNAs

This Example illustrates the identification of cDNA molecules encoding ovarian carcinoma antigens.

Anti-SCID mouse sera (generated against sera from SCID mice carrying late passage ovarian carcinoma) was pre-cleared of E. coli and phage antigens and used at a 1:200 dilution in a serological expression screen. The library screened was made from a SCID-derived human ovarian tumor (OV9334) using a directional RH oligo(dT) priming cDNA library construction kit and the λScreen vector (Novagen). A bacteriophage lambda screen was employed. Approximately 400,000 pfu of the amplified OV9334 library were screened.

196 positive clones were isolated. Certain sequences-that appear to be novel are provided in FIGS. 1A-1S and SEQ ID NOs:1 to 71. Three complete insert sequences are shown in FIGS. 2A-2C (SEQ ID NOs:72 to 74). Other clones having known sequences are presented in FIGS. 15 A- 15 EEE (SEQ ID NOs:82 to 310). Database searches identified the following sequences that were substantially identical to the sequences presented in FIGS. 15 A- 15 EEE.

These clones were further characterized using microarray technology to determine mRNA expression levels in a variety of tumor and normal tissues. Such analyses were performed using a Synteni (Palo Alto, Calif.) microarray, according to the manufacturer's instructions. PCR amplification products were arrayed on slides, with each product occupying a unique location in the array. mRNA was extracted from the tissue sample to be tested, reverse transcribed and fluorescent-labeled cDNA probes were generated. The microarrays were probed with the labeled cDNA probes and the slides were scanned to measure fluorescence intensity. Data was analyzed using Synteni's provided GEMtools software. The results for one clone (13695, also referred to as O8E) are shown in FIG. 3 .

Example 2

Identification of Ovarian Carcinoma cDNAs using Microarray Technology

This Example illustrates the identification of ovarian carcinoma polynucleotides by screening a microarray of cDNAs for ovarian tumor-specific expression. Such screens were performed using a Synteni (Palo Alto, Calif.) microarray, according to the manufacturer's instructions (and essentially as described by Schena et al., Proc. Natl. Acad. Sci. USA 93:10614-10619, 1996 and Heller et al., Proc. Natl. Acad. Sci. USA 94:2150-2155, 1997).

cDNA was generated from ovarian tumor tissue and from normal ovarian tissue, and a subtracted cDNA library was prepared and arrayed on the chip. The chip was then probed with fluorescent probes generated from normal and tumor cDNA. The slides were scanned and the fluorescence intensity was measured, and the data were analyzed using Synteni's GEMtools software. In general, sequences showing at least a 5-fold increase in expression in tumor cells (relative to normal cells) were considered ovarian tumor antigens.

Using such assays, an ovarian tumor antigen was identified that is a splice fusion between the human T-cell leukemia virus type I oncoprotein TAX (see Jin et al., Cell 93:81-91, 1998) and an extracellular matrix protein called osteonectin. A splice junction sequence exists at the fusion point. The sequence of this clone is presented in FIG. 4 and SEQ ID NO:75. Osteonectin, unspliced and unaltered, was also identified from such assays independently.

Further clones identified by this methods are referred to herein as 3f, 6b, 8e, 8h, 12c and 12h. Sequences of these clones are shown in FIGS. 5 to 9 and SEQ ID NOs:76 to 81. Microarray analyses were performed as described above, and are presented in FIGS. 10 to 14 .

From the foregoing it will be appreciated that, although specific embodiments of the invention have been described herein for purposes of illustration, various modifications may be made without deviating from the spirit and scope of the invention. Accordingly, the invention is not limited except as by the appended claims.

SUMMARY OF SEQUENCE LISTING

SEQ ID NOs:1-71 are ovarian carcinoma antigen polynucleotides shown in FIGS. 1A-1S .

SEQ ID NOs:72-74 are ovarian carcinoma antigen polynucleotides shown in FIGS. 2A-2C .

SEQ ID NO:75 is the ovarian carcinoma polynucleotide 3g (FIG. 4 ).

SEQ ID NO:76 is the ovarian carcinoma polynucleotide 3f (FIG. 5 ).

SEQ ID NO:77 is the ovarian carcinoma polynucleotide 6b (FIG. 6 ).

SEQ ID NO:78 is the ovarian carcinoma polynucleotide 8e (FIG. 7 A).

SEQ ID NO:79 is the ovarian carcinoma polynucleotide 8h (FIG. 7 B).

SEQ ID NO:80 is the ovarian carcinoma polynucleotide 2e (FIG. 8 ).

SEQ ID NO:81 is the ovarian carcinoma polynucleotide 12h (FIG. 9 ).

SEQ ID NOs:82-310 are ovarian carcinoma antigen polynucleotides shown in FIGS. 15 A- 15 EEE.

310 1 461 DNA Homo sapiens 1ttagagaggc acagaaggaa gaagagttaa aagcagcaaa gccgggtttt tttgttttgt 60tttgttttgt tttgttttga gatggagtct cactctgttg cccaagctgg agtacaacgg 120catgatctca gctcgctgca acctccgcct cccacgttca agtgattctc ctgcctcagc 180ctcccaagta gctgggatta caggcgcccg ccaccacgct cagctaattt tttttgtatt 240tttagtagag acagggtttc accaggttgg ccaggctgct cttgaactcc tgacctcagg 300tgatccaccc gcctcggcct cccaaagtgc tgggattaca ggcgtgagcc accacgcccg 360gcccccaaag ctgtttcttt tgtctttagc gtaaagctct cctgccatgc agtatctaca 420taactgacgt gactgccagc aagctcagtc actccgtggt c 461 2 540 DNA Homo sapiens 2taggatgtgt tggaccctct gtgtcaaaaa aaacctcaca aagaatcccc tgctcattac 60agaagaagat gcatttaaaa tatgggttat tttcaacttt ttatctgagg acaagtatcc 120attaattatt gtgtcagaag agattgaata cctgcttaag aagcttacag aagctatggg 180aggaggttgg cagcaagaac aatttgaaca ttataaaatc aactttgatg acagtaaaaa 240tggcctttct gcatgggaac ttattgagct tattggaaat ggacagttta gcaaaggcat 300ggaccggcag actgtgtcta tggcaattaa tgaagtcttt aatgaactta tattagatgt 360gttaaagcag ggttacatga tgaaaaaggg ccacagacgg aaaaactgga ctgaaagatg 420gtttgtacta aaacccaaca taatttctta ctatgtgagt gaggatctga aggataagaa 480aggagacatt ctcttggatg aaaattgctg tgtagagtcc ttgcctgaca aagatggaaa 540 3 461 DNA Homo sapiens 3ttagagaggc acagaaggaa gaagagttaa aagcagcaaa gccgggtttt tttgttttgt 60tttgttttgt tttgttttga gatggagtct cactctgttg cccaagctgg agtacaacgg 120catgatctca gctcgctgca acctccgcct cccacgttca agtgattctc ctgcctcagc 180ctcccaagta gctgggatta caggcgcccg ccaccacgct cagctaattt tttttgtatt 240tttagtagag acagggtttc accaggttgg ccaggctgct cttgaactcc tgacctcagg 300tgatccaccc gcctcggcct cccaaagtgc tgggattaca ggcgtgagcc accacgcccg 360gcccccaaag ctgtttcttt tgtctttagc gtaaagctct cctgccatgc agtatctaca 420taactgacgt gactgccagc aagctcagtc actccgtggt c 461 4 531 DNA Homo sapiens modified_base (454) Where n is a, c, g or t 4tctttttctt tcgatttcct tcaatttgtc acgtttgatt ttatgaagtt gttcaagggc 60taactgctgt gtattatagc tttctctgag ttccttcagc tgattgttaa atgaatccat 120ttctgagagc ttagatgcag tttctttttc aagagcatct aattgttctt taagtctttg 180gcataattct tccttttctg atgacttttt atgaagtaaa ctgatccctg aatcaggtgt 240gttactgagc tgcatgtttt taattctttc gtttaatagc tgcttctcag ggaccagata 300gataagctta ttttgatatt ccttaagctc ttgttgaagt tgtttgattt ccataatttc 360caggtcacac tgtttatcca aaacttctag ctcagtcttt tgtgtttgct ttctgatttg 420gacatcttgt agtctgcctg agatctgctg atgntttcca ttcactgctt ccagttccag 480gtggagactt tnctttctgg agctcagcct gacaatgcct tcttgntccc t 531 5 531 DNA Homo sapiens 5agccagatgg ctgagagctg caagaagaag tcaggatcat gatggctcag tttcccacag 60cgatgaatgg agggccaaat atgtgggcta ttacatctga agaacgtact aagcatgata 120aacagtttga taacctcaaa ccttcaggag gttacataac aggtgatcaa gcccgtactt 180ttttcctaca gtcaggtctg ccggccccgg ttttagctga aatatgggcc ttatcagatc 240tgaacaagga tgggaagatg gaccagcaag agttctctat agctatgaaa ctcatcaagt 300taaagttgca gggccaacag ctgcctgtag tcctccctcc tatcatgaaa caacccccta 360tgttctctcc actaatctct gctcgttttg ggatgggaag catgcccaat ctgtccattc 420atcagccatt gcctccagtt gcacctatag caacaccctt gtcttctgct acttcaggga 480ccagtattcc tcccctaatg atgcctgctc ccctagtgcc ttctgttagt a 531 6 531 DNA Homo sapiens 6aatagattta atgcagagtg tcaacttcaa ttgattgata gtggctgcct agagtgctgt 60gttgagtagg tttctgagga tgcaccctgg cttgaagaga aagactggca ggattaacaa 120tatctaaaat ctcacttgta ggagaaacca caggcaccag agctgccact ggtgctggca 180ccagctccac caaggccagc gaagagccca aatgtgagag tggcggtcag gctggcacca 240gcactgaagc caccactggt gctggcactg gcactggcac tgttattggt actggtactg 300gcaccagtgc tggcactgcc actctcttgg gctttggctt tagcttctgc tcccgcctgg 360atccgggctt tggcccaggg tccgatatca gcttcgtccc agttgcaggg cccggcagca 420ttctccgagc cgagcccaat gcccattcga gctctaatct cggccctagc cttggcttca 480gctgcagcct cagctgcagc cttcaaatcc gcttccatcg cctctcggta c 531 7 531 DNA Homo sapiens 7gccaagaaag cccgaaaggt gaagcatctg gatggggaag aggatggcag cagtgatcag 60agtcaggctt ctggaaccac aggtggccga agggtctcaa aggccctaat ggcctcaatg 120gcccgcaggg cttcaagggg tcccatagcc ttttgggccc gcagggcatc aaggactcgg 180ttggctgctt gggcccggag agccttgctc tccctgagat cacctaaagc ccgtaggggc 240aaggctcgcc gtagagctgc caagctccag tcatcccaag agcctgaagc accaccacct 300cgggatgtgg cccttttgca agggagggca aatgatttgg tgaagtacct tttggctaaa 360gaccagacga agattcccat caagcgctcg gacatgctga aggacatcat caaagaatac 420actgatgtgt accccgaaat cattgaacga gcaggctatt ccttggagaa ggtatttggg 480attcaattga aggaaattga taagaatgac cacttgtaca ttcttctcag c 531 8 531 DNA Homo sapiens modified_base (481) Where n is a, c, g or t 8gaggtctcac tatgttgccc aggctgttct tgaactcctg ggatcaagca atccacccat 60gttggtctcc aaaagtgctg ggatcatagg cgtgagccac ctcacccagc caccaatttt 120caatcaggaa gactttttcc ttcttcaaga agtgaagggt ttccagagta tagctacact 180attgcttgcc tgagggtgac tacaaaattg cttgctaaaa ggttaggatg ggtaaagaat 240tagattttct gaatgcaaaa ataaaatgtg aactaatgaa ctttaggtaa tacatattca 300taaaataatt attcacatat ttcctgattt atcacagaaa taatgtatga aatgctttga 360gtttcttgga gtaaactcca ttactcatcc caagaaacca tattataagt atcactgata 420ataagaacaa caggaccttg tcataaattc tggataagag aaatagtctc tgggtgtttg 480ntcttaattg ataaaattta cttgtccatc ttttagttca gaatcacaaa a 531 9 531 DNA Homo sapiens modified_base (528) Where n is a, c, g or t 9aagcggaaat gagaaaggag ggaaaatcat gtggtattga gcggaaaact gctggatgac 60agggctcagt cctgttggag aactctgggt ggtgctgtag aacagggcca ctcacagtgg 120ggtgcacaga ccagcacggc tctgtgacct gtttgttaca ggtccatgat gaggtaaaca 180atacactgag tataagggtt ggtttagaaa ctcttacagc aatttgacaa agtaatcttc 240tgtgcagtga atctaagaaa aaaattgggg ctgtatttgt atgttccttt ttttcatttc 300atgttctgag ttacctattt ttattgcatt ttacaaaagc atccttccat gaaggaccgg 360aagttaaaaa caaagcaggt cctttatcac agcactgtcg tagaacacag ttcagagtta 420tccacccaag gagccaggga gctgggctaa accaaagaat tttgcttttg gttaatcatc 480aggtacttga gttggaattg ttttaatccc atcattacca ggctggangt g 531 10 861 DNA Homo sapiens 10ccgcggctcc tgtccagacc ctgaccctcc ctcccaaggc tcaaccgtcc cccaacaacc 60gccagccttg tactgatgtc ggctgcgaga gcctgtgctt aagtaagaat caggccttat 120tggagacatt caagcaaagg ttggacaact acttttccag aacagaaagg aaactcatgc 180atcagaaaag gtgactaata aaggtaccag aagaatatgg ctgcacaaat accagaatct 240gatcagataa aacagtttaa ggaatttctg gggacctaca ataaacttac agagacctgc 300tttttggact gtgttagaga cttcacaaca agagaagtaa aacctgaaga gaccacctgt 360tcagaacatt gcttacagaa atatttaaaa atgacacaaa gaatatccat gagatttcag 420gaatatcata ttcagcagaa tgaagccctg gcagccaaag caggactcct tggccaacca 480cgatagagaa gtcctgatgg atgaactttt gatgaaagat tgccaacagc tgctttattg 540gaaatgagga ctcatctgat agaatcccct gaaagcagta gccaccatgt tcaaccatct 600gtcatgactg tttggcaaat ggaaaccgct ggagaaacaa aattgctatt taccaggaat 660aatcacaata gaaggtctta ttgttcagtg aaataataag atgcaacatt tgttgaggcc 720ttatgattca gcagcttggt cacttgatta gaaaaataaa ccattgtttc ttcaattgtg 780actgttaatt ttaaagcaac ttatgtgttc gatcatgtat gagatagaaa aatttttatt 840actcaaagta aaataaatgg a 861 11 541 DNA Homo sapiens 11gaaaaaaaat ataaaacaca cttttgcgaa aacggtggcc ctaaaagagg aaaagaattt 60caccaatata aatccaattt tatgaaaact gacaatttaa tccaagaatc acttttgtaa 120atgaagctag caagtgatga tatgataaaa taaacgtgga ggaaataaaa acacaagact 180tggcataaga tatatccact tttgatatta aacttgtgaa gcatattctt cgacaaattg 240tgaaagcgtt cctgatcttg cttgttctcc atttcaaata aggaggcata tcacatccca 300agagtaacag aaaaagaaaa aagacatttt tgcattttga gatgaaccaa agacacaaaa 360caaaacgaac aaagtgtcat gtctaattct agcctctgaa ataaaccttg aacatctcct 420acaaggcacc gtgatttttg taattctaac ctgaagaaat gtgatgactt ttgtggacat 480gaaaatcaga tgagaaaact gtggtctttc caaagcctga actcccctga aaacctttgc 540a 541 12 541 DNA Homo sapiens 12ctgggatcat ttctcttgat gtcataaaag actcttcttc ttcctcttca tcctcttctt 60catcctcttc tgtacagtgc tgccgggtac aacggctatc tttgtcttta tcctgagatg 120aagatgatgc ttctgtttct cctaccataa ctgaagaaat ttcgctggaa gtcgtttgac 180tggctgtttc tctgacttca ccttctttgt caaacctgag tctttttacc tcatgcccct 240cagcttccac agcatcttca tctggatgtt tatttttcaa agggctcact gaggaaactt 300ctgattcaga ggtcgaagag tcactgtgat ttttctcctc attttgctgc aaatttgcct 360ctttgctgtc tgtgctctca ggcaacccat ttgttgtcat gggggctgac aaagaaacct 420ttggtcgatt aagtggcctg ggtgtcccag gcccatttat attagacctc tcagtatagc 480ttggtgaatt tccaggaaac ataacaccat tcattcgatt taaactattg gaattggttt 540t 541 13 441 DNA Homo sapiens 13gagggttggt ggtagcggct tggggaggtg ctcgctctgt cggtcttgct ctctcgcacg 60cttcccccgg ctcccttcgt ttcccccccc cggtcgcctg cgtgccggag tgtgtgcgag 120ggagggggag ggcgtcgggg gggtgggggg aggcgttccg gtccccaaga gacccgcgga 180gggaggcgga ggctgtgagg gactccggga agccatggac gtcgagaggc tccaggaggc 240gctgaaagat tttgagaaga gggggaaaaa ggaagtttgt cctgtcctgg atcagtttct 300ttgtcatgta gccaagactg gagaaacaat gattcagtgg tcccaattta aaggctattt 360tattttcaaa ctggagaaag tgatggatga tttcagaact tcagctcctg agccaagagg 420tcctcccaac cctaatgtcg a 441 14 131 DNA Homo sapiens modified_base (126) Where n is a, c, g or t 14aagcaggcgg ctcccgcgct cgcagggccg tgccacctgc ccgcccgccc gctcgctcgc 60tcgcccgccg cgccgcgctg ccgaccgcca gcatgctgcc gagagtgggc tgccccgcgc 120tgccgntgcc g 131 15 692 DNA Homo sapiens 15atctcttgta tgccaaatat ttaatataaa tctttgaaac aagttcagat gaaataaaaa 60tcaaagtttg caaaaacgtg aagattaact taattgtcaa atattcctca ttgccccaaa 120tcagtatttt ttttatttct atgcaaaagt atgccttcaa actgcttaaa tgatatatga 180tatgatacac aaaccagttt tcaaatagta aagccagtca tcttgcaatt gtaagaaata 240ggtaaaagat tataagacac cttacacaca cacacacaca cacacacgtg tgcacgccaa 300tgacaaaaaa caatttggcc tctcctaaaa taagaacatg aagaccctta attgctgcca 360ggagggaaca ctgtgtcacc cctccctaca atccaggtag tttcctttaa tccaatagca 420aatctgggca tatttgagag gagtgattct gacagccacg ttgaaatcct gtggggaacc 480attcatgtcc acccactggt gccctgaaaa aatgccaata atttttcgct cccacttctg 540ctgctgtctc ttccacatcc tcacatagac cccagacccg ctggcccctg gctgggcatc 600gcattgctgg tagagcaagt cataggtctc gtctttgacg tcacagaagc gatacaccaa 660attgcctggt cggtcattgt cataaccaga ga 692 16 728 DNA Homo sapiens 16cagacggggt ttcactatgt tggctaggct ggtcttgaac tcctgacttc aggtgatctg 60cctgccttgg cctcccaaag tgctgggatt acaggcataa gccactgcgc ccggctgatc 120tgatggtttc ataaggcttt tccccctttt gctcagcact tctccttcct gccgccatgt 180gaagaaggac atgtttgctt ccccttccac cacgattgta agttgtttcc tgaggcctcc 240ccggccatgc tgaactgtga gtcaattaaa cctctttcct ttataaatta tccagttttg 300ggtatgtctt tattagtaga atgagaacag actaatacaa cccttaaagg agactgacgg 360agaggattct tcctggatcc cagcacttcc tctgaatgct actgacattc ttcttgagga 420ctttaaactg ggagatagaa aacagattcc atggctcagc agcctgagag cagggaggga 480gccaagctat agatgacatg ggcagcctcc cctgaggcca ggtgtggccg aacctgggca 540gtgctgccac ccaccccacc agggccaagt cctgtccttg gagagccaag cctcaatcac 600tgctagcctc aagtgtcccc aagccacagt ggctaggggg actcagggaa cagttcccag 660tctgccctac ttctcttacc tttacccctc atacctccaa agtagaccat gttcatgagg 720tccaaagg 728 17 531 DNA Homo sapiens modified_base (518) Where n is a, c, g or t 17aagcgaggaa gccactgcgg ctcctggctg aaaagcggcg ccaggctcgg gaacagaggg 60aacgcgaaga acaggagcgg aagctgcagg ctgaaaggga caagcgaatg cgagaggagc 120agctggcccg ggaggctgaa gcccgggctg aacgtgaggc cgaggcgcgg agacgggagg 180agcaggaggc tcgagagaag gcgcaggctg agcaggagga gcaggagcga ctgcagaagc 240agaaagagga agccgaagcc cggtcccggg aagaagctga gcgccagcgc caggagcggg 300aaaagcactt tcagaaggag gaacaggaga gacaagagcg aagaaagcgg ctggaggaga 360taatgaagag gactcggaaa tcagaagccg ccgaaaccaa gaagcaggat gcaaaggaga 420ccgcagctaa caattccggc ccagaccctt gtgaaagctg tagagactcg gccctctggg 480cttccagaaa ggattctatt gcagaaagga aggagctngg ccccccangg a 531 18 1041 DNA Homo sapiens modified_base (544) Where n is a, c, g or t 18ctctgtggaa aactgatgag gaatgaattt accattaccc atgttctcat ccccaagcaa 60agtgctgggt ctgattactg caacacagag aacgaagaag aacttttcct catacaggat 120cagcagggcc tcatcacact gggctggatt catactcacc ccacacagac cgcgtttctc 180tccagtgtcg acctacacac tcactgctct taccagatga tgttgccaga gtcagtagcc 240attgtttgct cccccaagtt ccaggaaact ggattcttta aactaactga ccatggacta 300gaggagattt cttcctgtcg ccagaaagga tttcatccac acagcaagga tccacctctg 360ttctgtagct gcagccacgt gactgttgtg gacagagcag tgaccatcac agaccttcga 420tgagcgtttg agtccaacac cttccaagaa caacaaaacc atatcagtgt actgtagccc 480cttaatttaa gctttctaga aagctttgga agtttttgta gatagtagaa aggggggcat 540cacntgagaa agagctgatt ttgtatttca ggtttgaaaa gaaataactg aacatatttt 600ttaggcaagt cagaaagaga acatggtcac ccaaaagcaa ctgtaactca gaaattaagt 660tactcagaaa ttaagtagct cagaaattaa gaaagaatgg tataatgaac ccccatatac 720ccttccttct ggattcacca attgttaaca tttttttcct ctcagctatc cttctaattt 780ctctctaatt tcaatttgtt tatatttacc tctgggctca ataagggcat ctgtgcagaa 840atttggaagc catttagaaa atcttttgga ttttcctgtg gtttatggca atatgaatgg 900agcttattac tggggtgagg gacagcttac tccatttgac cagattgttt ggctaacaca 960tcccgaagaa tgattttgtc aggaattatt gttatttaat aaatatttca ggatattttt 1020cctctacaat aaagtaacaa t 1041 19 1043 DNA Homo sapiens 19ctctgtggaa aactgatgag gaatgaattt accattaccc atgttctcat ccccaagcaa 60agtgctgggt ctgattactg caacacagag aacgaagaag aacttttcct catacaggat 120cagcagggcc tcatcacact gggctggatt catactcacc ccacacagac cgcgtttctc 180tccagtgtcg acctacacac tcactgctct taccagatga tgttgccaga gtcagtagcc 240attgtttgct cccccaagtt ccaggaaact ggattcttta aactaactga ccatggacta 300gaggagattt cttcctgtcg ccagaaagga tttcatccac acagcaagga tccacctctg 360ttctgtagct gcagccacgt gactgttgtg gacagagcag tgaccatcac agaccttcga 420tgagcgtttg agtccaacac cttccaagaa caacaaaacc atatcagtgt actgtagccc 480cttaatttaa gctttctaga aagctttgga agtttttgta gatagtagaa aggggggcat 540cacctgagaa agagctgatt ttgtatttca ggtttgaaaa gaaataactg aacatatttt 600ttaggcaagt cagaaagaga acatggtcac ccaaaagcaa ctgtaactca gaaattaagt 660tactcagaaa ttaagtagct cagaaattaa gaaagaatgg tataatgaac ccccatatac 720ccttccttct ggattcacca attgttaaca tttttttcct ctcagctatc cttctaattt 780ctctctaatt tcaatttgtt tatatttacc tctgggctca ataagggcat ctgtgcagaa 840atttggaagc catttagaaa atcttttgga ttttcctgtg gtttatggca atatgaatgg 900agcttattac tggggtgagg gacagcttac tccatttgac cagattgttt ggctaacaca 960tcccgaagaa tgattttgtc aggaattatt gttatttaat aaatatttca ggatattttt 1020cctctacaat aaagtaacaa tta 1043 20 448 DNA Homo sapiens 20ggacgacaag gccatggcga tatcggatcc gaattcaagc ctttggaatt aaataaacct 60ggaacaggga aggtgaaagt tggagtgaga tgtcttccat atctatacct ttgtgcacag 120ttgaatggga actgtttggg tttagggcat cttagagttg attgatggaa aaagcagaca 180ggaactggtg ggaggtcaag tggggaagtt ggtgaatgtg gaataactta cctttgtgct 240ccacttaaac cagatgtgtt gcagctttcc tgacatgcaa ggatctactt taattccaca 300ctctcattaa taaattgaat aaaagggaat gttttggcac ctgatataat ctgccaggct 360atgtgacagt aggaaggaat ggtttcccct aacaagccca atgcactggt ctgactttat 420aaattattta ataaaatgaa ctattatc 448 21 411 DNA Homo sapiens 21ggcagtgaca ttcaccatca tgggaaccac cttccctttt cttcaggatt ctctgtagtg 60gaagagagca cccagtgttg ggctgaaaac atctgaaagt agggagaaga acctaaaata 120atcagtatct cagagggctc taaggtgcca agaagtctca ctggacattt aagtgccaac 180aaaggcatac tttcggaatc gccaagtcaa aactttctaa cttctgtctc tctcagagac 240aagtgagact caagagtcta ctgctttagt ggcaactaca gaaaactggt gttacccaga 300aaaacaggag caattagaaa tggttccaat atttcaaagc tccgcaaaca ggatgtgctt 360tcctttgccc atttagggtt tcttctcttt cctttctctt tattaaccac t 411 22 896 DNA Homo sapiens modified_base (230) Where n is a, c, g or t 22tgcgctgaaa acaacggcct cctttactgt taaaatgcag ccacaggtgc ttagccgtgg 60gcatctcaac caccagcctc tgtggggggc aggtgggcgt ccctgtgggc ctctgggccc 120acgtccagcc tctgtcctct gccttccgtt cttcgacagt gttcccggca tccctggtca 180cttggtactt ggcgtgggcc tcctgtgctg ctccagcagc tcctccaggn ggtcggcccg 240cttcaccgca gcctcatgtt gtgtccggag gctgctcacg gcctcctcct tcctcgcgag 300ggctgtcttc accctccggn gcacctcctc cagctccagc tgctggcggg cctgcagcgt 360ggccagctcg gccttggcct gccgcgtctc ctcctcarag gctgccagcc ggtcctcgaa 420ctcctggcgg atcacctggg ccaggttgct gcgctcgcta gaaagctgct cgttcaccgc 480ctgcgcatcc tccagcgccc gctccttctg ccgcacaagg ccctgcagac gcagattctc 540gccctcggcc tccccaagct ggcccttcag ctccgagcac cgctcctgaa gcttccgctc 600cgactgctcc agctcggaga gctcggcctc gtacttgtcc cgtaagcgct tgatgcggct 660ctcggcagcc ttctcactct cctccttggc cagcgccatg tcggcctcca gccggtgaat 720gaccagctca atctccttgt cccggccttt ccggatttct tccctcagct cctgttcccg 780gttcagcagc cacgcctcct ccttcctggt gcggccggcc tcccacgcct gcctctccag 840ctccagctgc tgcttcaggg tattcagctc catctggcgg gcctgcagcg tggcca 896 23 111 DNA Homo sapiens 23caacttatta cttgaaatta taatatagcc tgtccgtttg ctgtttccag gctgtgatat 60attttcctag tggtttgact ttaaaaataa ataaggttta attttctccc c 111 24 531 DNA Homo sapiens modified_base (472) Where n is a, c, g or t 24tgcaagtcac gggagtttat ttatttaatt tttttcccca gatggagact ctgtcgccca 60ggctggagtg caatggtgtg atcttggctc actgcaacct ccacctcctg ggttcaagcg 120attctcctgc cacagcctcc cgagtagctg ggattacagg tgcccgccac cacacccagc 180taatttttat atttttagta aagacagggt ttccccatgt tggccaggct ggtcttgaac 240ttctgacctc aggtgatcca cctgcctcgg cctcccaaag tgttgggatt acaggcgtga 300gctacccgtg cctggccagc cactggagtt taaaggacag tcatgttggc tccagcctaa 360ggcggcattt tcccccatca gaaagcccgc ggctcctgta cctcaaaata gggcacctgt 420aaagtcagtc agtgaagtct ctgctctaac tggccacccg gggccattgg cntctgacac 480agccttgcca ggangcctgc atctgcaaaa gaaaagttca cttcctttcc g 531 25 471 DNA Homo sapiens modified_base (377) Where n is a, c, g or t 25cagagaatct kagaaagatg tcgcgttttc ttttaatgaa tgagagaagc ccatttgtat 60ccctgaatca ttgagaaaag gcggcggtgg cgacagcggc gacctaggga tcgatctgga 120gggacttggg gagcgtgcag agacctctag ctcgagcgcg agggacctcc cgccgggatg 180cctggggagc agatggaccc tactggaagt cagttggatt cagatttctc tcagcaagat 240actccttgcc tgataattga agattctcag cctgaaagcc aggttctaga ggatgattct 300ggttctcact tcagtatgct atctcgacac cttcctaatc tccagacgca caaagaaaat 360cctgtgttgg atgttgngtc caatccttga acaaacagct ggagaagaac gaggagaccg 420gtaatagtgg gttcaatgaa catttgaaag aaaaccaggt tgcagaccct g 471 26 541 DNA Homo sapiens 26gactgtcctg aacaagggac ctctgaccag agagctgcag gagatgcaga gtggtggcag 60gagtggaagc caaagaacac ccaccttcct cccttgaagg agtagagcaa ccatcagaag 120atactgtttt attgctctgg tcaaacaagt cttcctgagt tgacaaaacc tcaggctctg 180gtgacttctg aatctgcagt ccactttcca taagttcttg tgcagacaac tgttcttttg 240cttccatagc agcaacagat gctttggggc taaaaggcat gtcctctgac cttgcaggtg 300gtggattttg ctcttttaca acatgtacat ccttactggg ctgtgctgtc acagggatgt 360ccttgctgga ctgttctgct atggggatat cttcgttgga ctgttcttca tgcttaattg 420cagtattagc atccacatca gacagcctgg tataaccaga gttggtggtt actgattgta 480gctgctcttt gtccacttca tatggcacaa gtattttcct caacatcctg gctctgggaa 540g 541 27 461 DNA Homo sapiens modified_base (367) Where n is a, c, g or t 27gaaatgtata tttaatcatt ctcttgaacg atcagaactc traaatcagt tttctataac 60arcatgtaat acagtcaccg tggctccaag gtccaggaag gcagtggtta acacatgaag 120agtgtgggaa gggggctgga aacaaagtat tcttttcctt caaagcttca ttcctcaagg 180cctcaattca agcagtcatt gtccttgctt tcaaaagtct gtgtgtgctt catggaaggt 240atatgtttgt tgccttaatt tgaattgtgg ccaggaaggg tctggagatc taaattcaga 300gtaagaaaac ctgagctaga actcaggcat ttctcttaca gaacttggct tgcagggtag 360aatgaangga aagaaactta gaagctcaac aagctgaaga taatcccatc aggcatttcc 420cataggcctt gcaactctgt tcactgagag atgttatcct g 461 28 541 DNA Homo sapiens 28agtctggagt gagcaaacaa gagcaagaaa caarragaag ccaaaagcag aaggctccaa 60tatgaacaag ataaatctat cttcaaagac atattagaag ttgggaaaat aattcatgtg 120aactagacaa gtgtgttaag agtgataagt aaaatgcacg tggagacaag tgcatcccca 180gatctcaggg acctccccct gcctgtcacc tggggagtga gaggacagga tagtgcatgt 240tctttgtctc tgaattttta gttatatgtg ctgtaatgtt gctctgagga agcccctgga 300aagtctatcc caacatatcc acatcttata ttccacaaat taagctgtag tatgtaccct 360aagacgctgc taattgactg ccacttcgca actcaggggc ggctgcattt tagtaatggg 420tcaaatgatt cactttttat gatgcttccc aaggtgcctt ggcttctctt cccaactgac 480aaatgcccaa gttgagaaaa atgatcataa ttttagcata aaccgagcaa tcggcgaccc 540c 541 29 411 DNA Homo sapiens 29tagctgtctt cctcactctt atggcaatga ccccatatct taatggatta agataatgaa 60agtgtatttc ttacactctg tatctatcac cagaagctga ggtgatagcc cgcttgtcat 120tgtcatccat attctgggac tcaggcggga actttctgga atattgccag ggagcatggc 180agaggggcac agtgcattct gggggaatgc acattggctc agcctgggta atgagtgata 240tacattacct ctgttcacaa ctcattgccc agcaccagtc acaaggcccc accaaatacc 300agagcccaag aaatgtagtc ctgttgatat ggttttgctg tgtcccaacc caaatctcat 360cttgaattgt aagctcccat aattcccatg tgttgtggga gggacctggt g 411 30 511 DNA Homo sapiens 30atcatgagga tgttaccaaa gggatggtac taaaccattt gtattcgtct gttttcacac 60tgctttgaag atactacctg agactgggta atttataaac aaaagagatt taattgactc 120acagttctgc atggctgaag aggcctcagg aaacttacag tcatggtgga aggcaaagga 180ggagcaaggc atgtcttaca tgtcagtagg agagagagcg agagcaggag aacctgccac 240ttataaacca ttcagatctc ataactccct atcatgagaa aaacatggag gaaaccaccc 300tcatgatcca atcacctccc gccaggtccc tccctcgaca cgtggggatt ataattcagg 360attagaggga cacagagaca aaccatatca tcattcatga gaaatccacc ctcatagtcc 420aatcagctcc taccaggccc cacctccaac actggggatt gcaattcaac atgagatttg 480gatggggaca cagattcaaa ccatatcata c 511 31 827 DNA Homo sapiens 31catggccttt ctccttagag gccagaggtg ctgccctggc tgggagtgaa gctccaggca 60ctaccagctt tcctgatttt cccgtttggt ccatgtgaag agctaccacg agccccagcc 120tcacagtgtc cactcaaggg cagcttggtc ctcttgtcct gcagaggcag gctggtgtga 180ccctgggaac ttgacccggg aacaacaggt ggcccagagt gagtgtggcc tggcccctca 240acctagtgtc cgtcctcctc tctcctggag ccagtcttga gtttaaaggc attaagtgtt 300agatacaagc tccttgtggc tggaaaaaca cccctctgct gataaagctc agggggcact 360gaggaagcag aggccccttg ggggtgccct cctgaagaga gcgtcaggcc atcagctctg 420tccctctggt gctcccacgt ctgttcctca ccctccatct ctgggagcag ctgcacctga 480ctggccacgc gggggcagtg gaggcacagg ctcagggtgg ccgggctacc tggcacccta 540tggcttacaa agtagagttg gcccagtttc cttccacctg aggggagcac tctgactcct 600aacagtcttc cttgccctgc catcatctgg ggtggctggc tgtcaagaaa ggccgggcat 660gctttctaaa cacagccaca ggaggcttgt agggcatctt ccaggtgggg aaacagtctt 720agataagtaa ggtgacttgc ctaaggcctc ccagcaccct tgatcttgga gtctcacagc 780agactgcatg tsaacaactg gaaccgaaaa catgcctcag tataaaa 827 32 291 DNA Homo sapiens 32ccagaacctc cttctctttg gagaatgggg aggcctcttg gagacacaga gggtttcacc 60ttggatgacc tctagagaaa ttgcccaaga agcccacctt ctggtcccaa cctgcagacc 120ccacagcagt cagttggtca ggccctgctg tagaaggtca cttggctcca ttgcctgctt 180ccaaccaatg ggcaggagag aaggccttta tttctcgccc acccattctc ctgtaccagc 240acctccgttt tcagtcagyg ttgtccagca acggtaccgt ttacacagtc a 291 33 491 DNA Homo sapiens 33tgcatgtagt tttatttatg tgttttsgtc tggaaaacca agtgtcccag cagcatgact 60gaacatcact cacttcccct acttgatcta caaggccaac gccgagagcc cagaccagga 120ttccaaacac actgcacgag aatattgtgg atccgctgtc aggtaagtgt ccgtcactga 180cccaracgct gttacgtggc acatgactgt acagtgccac gtaacagcac tgtacttttc 240tcccatgaac agttacctgc catgtatcta catgattcag aacattttga acagttaatt 300ctgacacttg aataatccca tcaaaaaccg taaaatcact ttgatgtttg taacgacaac 360atagcatcac tttacgacag aatcatctgg aaaaacagaa caacgaatac atacatctta 420aaaaatgctg gggtgggcca ggcacagctt cacgcctgta atcccagcac tttgggaggc 480ttaagcgggt g 491 34 521 DNA Homo sapiens modified_base (453) Where n is a, c, g or t 34tggggcggaa agaagccaag gccaaggagc tggtgcggca gctgcagctg gaggccgagg 60agcagaggaa gcagaagaag cggcagagtg tgtcgggcct gcacagatac cttcacttgc 120tggatggaaa tgaaaattac ccgtgtcttg tggatgcaga cggtgatgtg atttccttcc 180caccaataac caacagtgag aagacaaagg ttaagaaaac gacttctgat ttgtttttgg 240aagtaacaag tgccaccagt ctgcagattt gcaaggatgt catggatgcc ctcattctga 300aaatggcaag aaatgaaaaa gtacacttta gaaaataaag aggaaggatc actctcagat 360actgaagccg atgcagtctc tggacaactt ccagatccca caacgaatcc cagtgctgga 420aaggacgggc ccttccttct ggtggtggaa cangtcccgg tggtggatct tggaanggaa 480cctgaangtg gtgtaccccg tccaaggccg accttggcca c 521 35 161 DNA Homo sapiens modified_base (18) Where n is a, c, g or t 35tcccgcgctc gcagggcncg tgccacctgc cygtccgccc gctcgctcgc tcgcccgccg 60cgccgcgctg ccgaccgyca gcatgctgcc gagagtgggc tgccccgcgc tgccgctgcc 120gccgccgccg ctgctgccgc tgctgccgct gctgctgctg c 161 36 341 DNA Homo sapiens 36ggcgggtagg catggaactg agaagaacga agaagctttc agactacgtg gggaagaatg 60aaaaaaccaa aattatcgcc aagattcagc aaaggggaca gggagctcca gcccgagagc 120ctattattag cagtgaggag cagaagcagc tgatgctgta ctatcacaga agacaagagg 180agctcaagag attggaagaa aatgatgatg atgcctattt aaactcacca tgggcggata 240acactgcttt gaaaagacat tttcatggag tgaaagacat aaagtggaga ccaagatgaa 300gttcaccagc tgatgacact tccaaagaga ttagctcacc t 341 37 521 DNA Homo sapiens modified_base (516) Where n is a, c, g or t 37tctgaaggtt aaatgtttca tctaaatagg gataatgrta aacacctata gcatagagtt 60gtttgagatt aaatgagata atacatgtaa aattatgtgc ctggcataca gcaagattgt 120tgttgttgtt gatgatgatg atgatgatga taatattttt ctatccccag tgcacaactg 180cttgaaccta ttagataatc aatacatgtt tcttgaactg agatcaattt ccccatgttg 240tctgactgat gaagccctac attttcttct agaggagatg acatttgagc aagatcttaa 300agaaaatcag atgccttcac ctgaccactg cttggtgatc ccatggcact ttgtacatct 360ctccattagc tctcatctca ccagcccatc attattgtat gtgctgcctt ctgaagcttg 420cagctggcta ccatcmggta gaataaaaat catcctttca taaaatagtg accctccttt 480tttatttgca tttcccaaag ccaagcaccg tggganggta g 521 38 461 DNA Homo sapiens 38tatgaagaag ggaaaagaag ataatttgtg aaagaaatgg gtccagttac tagtctttga 60aaagggtcag tctgtagctc ttcttaatga gaataggcag ctttcagttg ctcagggtca 120gatttcctta gtggtgtatc taatcacagg aaacatctgt ggttccctcc agtctctttc 180tgggggactt gggcccactt ctcatttcat ttaattagag gaaatagaac tcaaagtaca 240atttactgtt gtttaacaat gccacaaaga catggttggg agctatttct tgatttgtgt 300aaaatgctgt ttttgtgtgc tcataatggt tccaaaaatt gggtgctggc caaagagaga 360tactgttaca gaagccagca agaagacctc tgttcattca cacccccggg gatatcagga 420attgactcca gtgtgtgcaa atccagtttg gcctatcttc t 461 39 769 DNA Homo sapiens 39tgagggactg attggtttgc tctctgctat tcaattcccc aagcccactt gttcctgcag 60cgtcctcctt ctcattccct ttagttgtac cctctctttc atctgagacc tttccttctt 120gatgtcgcct tttcttcttc ttgctttttc tgatgttctg ctcagcatgt tctgggtgct 180tctcatctgc atcattcctt tcagatgctg tagcttcttc ctcctctttc tgcctccttt 240tctttttctt ttttttgggg ggcttgctct ctgactgcag ttgaggggcc ccagggtcct 300ggcctttgag acgagccagg aaggcctgct cctgggcctc taggcgagca agcttggcct 360tcattgtgat cccaagacgg gcagccttgt gtgctgttcg cccctcacag gcttggagca 420gcatctcatc agtcagaatc tttggggact tggacccctg gttgtcgtca tcactgcagc 480tctccaagtc tttgtttggc ttctctccac ctgaagtcaa tgtagccatc ttcacaaact 540tctgatacag caagttgggc ttgggatgat tataacgggt ggtctcctta gaaaggctcc 600ttatctgtac tccatcctgc ccagtttcca ctaccaagtt ggccgcagtc ttgttgaaga 660gctcattcca ccagtggttt gtgaactcct tggcagggtc atgtcctacc ccatgagtgt 720cttgcttcag ygtcaccctg agagcctgag tgataccatt ctccttccg 769 40 292 DNA Homo sapiens 40gacaacatga aataaatcct agaggacaaa attaaactca atagagtgta gtctagttaa 60aaactcgaaa aatgagcaag tctggtggga gtggaggaag ggctatacta taaatccaag 120tgggcctcct gatcttaaca agccatgctc attatacaca tctctgaact ggacatacca 180cctttacgca ggaaacaggg cttggaactt ctaagggaaa ttaacatgca ccacccacat 240ctaacctacc tgccgggtag gtaccatccc tgcttcgctg aaatcagtgc tc 292 41 406 DNA Homo sapiens 41ttggaattaa ataaacctgg aacagggaag gtgaaagttg gagtgagatg tcttccatat 60ctataccttt gtgcacagtt gaatgggaac tgtttgggtt tagggcatct tagagttgat 120tgatggaaaa agcagacagg aactggtggg aggtcaagtg gggaagttgg tgaatgtgga 180ataacttacc tttgtgctcc acttaaacca gatgtgttgc agctttcctg acatgcaagg 240atctacttta attccacact ctcattaata aattgaataa aagggaatgt tttggcacct 300gatataatct gccaggctat gtgacagtag gaaggaatgg tttcccctaa caagcccaat 360gcactggtct gactttataa attatttaat aaaatgaact attatc 406 42 381 DNA Homo sapiens 42aaactggacc tgcaacaggg acatgaattt actgcarggt ctgagcaagc tcagcccctc 60tacctcaggg ccccacagcc atgactacct cccccaggag cgggagggtg aagggggcct 120gtctctgcaa gtggagccag agtggaggaa tgagctctga agacacagca cccagccttc 180tcgcaccagc caagccttaa ctgcctgcct gaccctgaac cagaacccag ctgaactgcc 240cctccaaggg acaggaaggc tgggggaggg agtttacaac ccaagccatt ccaccccctc 300ccctgctggg gagaatgaca catcaagctg ctaacaattg ggggaagggg aaggaagaaa 360actctgaaaa caaaatcttg t 381 43 451 DNA Homo sapiens 43catgcgtttc accactgttg gccaggctgg tctcgaactc ctggcctcaa gcaatccacc 60cgcctcagcc tccaaaagtg ctgggattac agatgtgagc catggcacca tgccaaaagg 120ctatattcct ggctctgtgt ttccgagact gcttttaatc ccaacttctc tacatttaga 180ttaaaaaata ttttattcat ggtcaatctg gaacataatt actgcatctt aagtttccac 240tgatgtatat agaaggctaa aggcacaatt tttatcaaat ctagtagagt aaccaaacat 300aaaatcatta attactttca acttaataac taattgacat tcctcaaaag agctgttttc 360aatcctgata ggttctttat tttttcaaaa tatatttgcc atgggatgct aatttgcaat 420aaggcgcata atgagaatac cccaaactgg a 451 44 521 DNA Homo sapiens 44gttggacccc cagggactgg aaagacactt cttgcccgag ctgtggcggg agaagctgat 60gttccttttt attatgcttc tggatccgaa tttgatgaga tgtttgtggg tgtgggagcc 120agccgtatca gaaatctttt tagggaagca aaggcgaatg ctccttgtgt tatatttatt 180gatgaattag attctgttgg tgggaagaga attgaatctc caatgcatcc atattcaagg 240cagaccataa atcaacttct tgctgaaatg gatggtttta aacccaatga aggagttatc 300ataataggag ccacaaactt cccagaggca ttagataatg ccttaatacc gtcctggtcg 360ttttgacatg caagttacag ttccaaggcc agatgtaaaa ggtcgaacag aaattttgaa 420atggtatctc aataaaataa agtttgatca atcccgttga tccagaaatt atagcctcga 480ggtactggtg gcttttccgg aagcagagtt gggagaatct t 521 45 585 DNA Homo sapiens 45gcctacaaca tccagaaaga gtctaccctg cacctggtgc tscgtctcag aggtgggatg 60cagatcttcg tgaagaccct gactggtaag accatcactc tcgaagtgga gccgagtgac 120accatygaga acgtcaaagc aaagatccar gacaaggaag gcrtycctcc tgaccagcag 180aggttgatct ttgccggaaa gcagctggaa gatggdcgca ccctgtctga ctacaacatc 240cagaaagagt cyaccctgca cctggtgctc cgtctcagag gtgggatgca ratcttcgtg 300aagaccctga ctggtaagac catcaccctc gaggtggagc ccagtgacac catcgagaat 360gtcaaggcaa agatccaaga taaggaaggc atccctcctg atcagcagag gttgatcttt 420gctgggaaac agctggaaga tggacgcacc ctgtctgact acaacatcca gaaagagtcc 480actctgcact tggtcctgcg cttgaggggg ggtgtctaag tttccccttt taaggtttcm 540acaaatttca ttgcactttc ctttcaataa agttgttgca ttccc 585 46 481 DNA Homo sapiens 46gaactgggcc ctgagcccaa gtcatgcctt gtgtccgcat ctgccgtgtc acctctgtkc 60ctgcccctca cccctccctc ctggtcttct gagccagcac catctccaaa tagcctattc 120cttcctgcaa atcacacaca catgcgggcc acacatacct gctgccctgg agatggggaa 180gtaggagaga tgaatagagg cccatacatt gtacagaagg aggggcaggt gcagataaaa 240gcagcagacc cagcggcagc tgaggtgcat ggagcacggt tggggccggc attgggctga 300gcacctgatg ggcctcatct cgtgaatcct cgaggcagcg ccacagcaga ggagttaagt 360ggcacctggg ccgagcagag caggagactg agggtcagag tggaggctaa gctgccctgg 420aactcctcaa tcttgcctgc cccctagtat gaagccccct tcctgcccct acaattcctg 480a 481 47 461 DNA Homo sapiens modified_base (128) Where n is a, c, g or t 47atggatctta ctttgccacc caggttggag tgcagtgctg caatcttggc tcactgcagc 60cttaacctcc caggctcaag ctatcctcct gccaaagcct tccacatagc tgggactaca 120ggtacacngc caccacaccc agctaaaatt tttgtatttt ttgtagagac gggatctcgc 180cacgttgccc aggctggtcc catcctgacc tcaagcagat ctgcccacct cagcccccca 240acgtgctagg attacaggcg tgagccaccg cacccagcct ttgttttgct tttaatggaa 300tcaccagttc ccctccgtgt ctcagcagca gctgtgagaa atgctttgca tctgtgacct 360ttatgaaggg gaacttccat gctgaatgag ggtaggatta catgctcctg tttcccgggg 420gtcaagaaag cctcagactc cagcatgata agcagggtga g 461 48 571 DNA Homo sapiens 48ataggggctt taaggaggga attcaggttc aatgaggtcg taaggccagg gctcttatcc 60agtaagactg gggtccttag atgagaaaga gacacccgag gtccttctct ctgccgtgtg 120aggatgcatc aagaaggcgg ccgtctgcaa gcgaaggaga ggccgcacca gaaaccgaca 180ccttcatctt ggacttgcag cctctagaac tgagaaaata actgtctgtt ggttaagcca 240cccagtttgt agtattctct tatggcttcc taagcagact aacaaacaaa cacccaaaat 300taactgatgg cttcgctgtc ttctgtaaaa attgctatga gagaactttt cactcactgt 360tttgcagttt ctccctcagt ccctggttct ttcttctcac ataatcccaa tttcaattta 420tagttcatgg cccaggcaga gtcattcatc acggcatctc ctgagctaaa ccagcacctg 480ctctgctcac ttcttgactg gctgctcatc atcagccctc ttgcagagat ttcatttcct 540cccgtgccag gtacttcacg caccaagctc a 571 49 511 DNA Homo sapiens 49ggataatgaa gttgttttat ttagcttgga caaaaaggca tattcctcta ttttcttata 60caacaaatat ccccaaaata aagcaagcat atatatcttg aatgtgtaat aatccagtga 120taaacaagag cagtacttta aaagaaaaaa aaatatgtat ttctgtcagg ttaaaatgag 180aatcaaaacc atttactctg ctaactcatt attttttgct ttctttttgg ttaagagagg 240caatgcaata cactgaaaaa ggtttttatc ttatctggca ttggaattag acatattcaa 300accccagccc ccatttccaa actttaagac cacaaacaag taatttactt ttctgaacat 360tggttttttc tggaaaatgg gaattataaa atagactttg cagactctta tgagattaaa 420taagataatg tatgaaattc tttcttcttt tttacttctt tttccttttt gagatggagt 480ctcaccccgt cacccaggct ggagtacagt g 511 50 561 DNA Homo sapiens 50ccactgcact ccagcctggg tgacggagtg agactctgtc tcaaaaaaac aaacaaacaa 60acaaacaaaa aactgaaaag gaaatagagt tcctctttcc tcatatatga atatattatt 120tcaacagatt gttgatcacc taccatatgc ttggtattgt tctaattgct ggggatacag 180caagaggttc tgcagaactt catggagcat gaaagtaaat aaacaaagtt aatttcaagg 240ccaggcatgg ttgctcacac ctttagtccc agcactttgg gaggctgagg caggtggatc 300acttgggccc aggagttcaa ggctgcagtg agccaagatt gtgccactac tctccaggct 360gggcaacaga gcaagaccct gtctcagggg gaacaaaaag ttaatttcag attttgttaa 420gtgctgtaaa ggaagtaaat aggttgatat tcaagagagc acctgaaggc caggcgtggt 480ggctcacgcc tgtggtctaa cgctttggga agcccgagcg ggcggatcac aaggtcagga 540gaattttggc caggcatggt g 561 51 451 DNA Homo sapiens 51agaatccatt tattgggttt taaactagtt acacaactga aatcagtttg gcactacttt 60atacagggat tacgcctgtg tatgccgaca cttaaatact gtaccaggac cactgctgtg 120cttaggtctg tattcagtca ttcagcatgt agatactaaa aatatactgt agtgttcctt 180taaggaagac tgtacagggt gtgttgcaag atgacattca ccaatttgtg aattatttca 240acccagaaga tacctttcac tctataaact tgtcataggc aaacatgtgg tgttagcatt 300gagagatgca cacaaaaatg ttacataaaa gttcagacat tctaatgata agtgaactga 360aaaaaaaaaa aaccccacat ctcaattttt gtaacaagat aaagaaaata atttaaaaac 420acaaaaaatg gcattcagtg ggtacaaagc c 451 52 682 DNA Homo sapiens 52caaatattta atataaatct ttgaaacaag ttcagakgaa ataaaaatca aagtttgcaa 60aaacgtgaag attaacttaa ttgtcaaata ttcctcattg ccccaaatca gtattttttt 120tatttctatg caaaagtatg ccttcaaact gcttaaatga tatatgatat gatacacaaa 180ccagttttca aatagtaaag ccagtcatct tgcaattgta agaaataggt aaaagattat 240aagacacctt acacacacac acacacacac acacacacgt gtgcaccgcc aatgacaaaa 300aacaatttgg cctctcctaa aataagaaca tgaagaccct taattgctgc caggagggaa 360cactgtgtca cccctcccta caatccaggt agtttccttt aatccaatag caaatctggg 420catatttgag aggagtgatt ctgacagcca csgttgaaat cctgtgggga accattcatg 480tccacccact ggtgccctga aaaaatgcca ataatttttc gctcccactt ctgctgctgt 540ctcttccaca tcctcacata gaccccagac ccgctggccc ctggctgggc atcgcattgc 600tggtagagca agtcataggt ctcgtctttg acgtcacaga agcgatacac caaattgcct 660ggtcggtcat tgtcataacc ag 682 53 311 DNA Homo sapiens modified_base (208) Where n is a, c, g or t 53tttgacttta gtaggggtct gaactattta ttttactttg ccmgtaatat ttaraccyta 60tatatctttc attatgccat cttatcttct aatgbcaagg gaacagwtgc taamctggct 120tctgcattwa tcacattaaa aatggctttc ttggaaaatc ttcttgatat gaataaagga 180tcttttavag ccatcattta aagcmggntt ctctccaaca cgagtctgct sasggggggk 240gagctgtgaa ctctggctga aggctttccc atacacactg caatgacmtg gtttctgacc 300agbgtgagtt a 311 54 561 DNA Homo sapiens 54agagaagccc cataaatgca atcagtgtgg gaaggccttc agtcagagct caagcctttt 60cctccatcat cgggttcata ctggagagaa accctatgta tgtaatgaat gcggcagagc 120ctttggtttt aactctcatc ttactgaaca cgtaaggatt cacacaggag aaaaacccta 180tgtttgtaat gagtgcggca aagcctttcg tcggagttcc actcttgttc agcatcgaag 240agttcacact ggggagaagc cctaccagtg cgttgaatgt gggaaagctt tcagccagag 300ctcccagctc accctacatc agccgagttc acactggaga gaagccctat gactgtggtg 360actgtgggaa ggccttcagc cggaggtcaa ccctcattca gcatcagaaa gttcacagcg 420gagagactcg taagtgcaga aaacatggtc cagcctttgt tcatggctcc agcctcacag 480cagatggaca gattcccact ggagagaagc acggcagaac ctttaaccat ggtgcaaatc 540tcattctgcg ctggacagtt c 561 55 811 DNA Homo sapiens 55gagacagggt ctcactttgt cacccaggct ggaatgcagt ggtgcgatct tacgtagctc 60actgcagccc tgacctcctg gactcaaaca attctcctgc ctcagccctg caagtagctg 120ggactgtggg tgcatgccac catgcctggc taacttttgt agtttttgta aagatggggt 180tttgccatgt tgcacatgct ggtcttgaac tcctgagctc aaacgatctg cccacctcgg 240cctcccagaa tgttgggatt acaggggtaa accaccacgc ctggccccat tagggtattc 300ttagcatcca cttgctcact gagattaatc ataagagatg ataagcactg gaagaaaaaa 360atttttacta ggctttggat atttttttcc tttttcagct ttatacagag gattggatct 420ttagttttcc tttaactgat aataaaacat tgaaaggaaa taagtttacc tgagattcac 480agagataacc ggcatcactc ccttgctcaa ttccagtctt taccacatca attattttca 540gaggtgcagg ataaaggcct ttagtctgct ttcgcacttt ttcttccact tttttgtaaa 600cctgttgcct gacaaatgga attgacagcg tatgccatga ctattccatt tgtcaggcat 660acgctgtcaa tttttccacc aatcccttgt ctctctttgg agagatcttc ttatcagcta 720gtcctttggc aaaagtaatt gcaacttctt ctaggtattc tattgtccgt tccactggtg 780gaacccctgg gaccaggact aaaacctcca g 811 56 591 DNA Homo sapiens modified_base (45) Where n is a, c, g or t 56atctcatata tatatttctt cctgacttta tttgcttgct tctgncacgc atttaaaata 60tcacagagac caaaatagag cggctttctg gtggaacgca tggcagtcac aggacaaaat 120acaaaactag ggggctctgt cttctcatac atcatacaat tttcaagtat tttttttatg 180tacaaagagc tactctatct gaaaaaaaat taaaaaataa atgagacaag atagtttatg 240catcctagga agaaagaatg ggaagaaaga acggggcagt tgggtacaga ttcctgtccc 300ctgttcccag ggaccactac cttcctgcca ctgagttccc ccacagcctc acccatcatg 360tcacagggca agtgccaggg taggtgggga ccagtggaga caggaaccag caacatactt 420tggcctggaa gataaggaga aagtctcaga aacacactgg tgggaagcaa tcccacnggc 480cgtgccccan gagcttccca cctgctgctg gctccctggg tggctttggg aacagcttgg 540gcaggccctt ttgggtgggg nccaactggg cctttgggcc cgtgtggaaa g 591 57 481 DNA Homo sapiens 57aaacattgag atggaatgat agggtttccc agaatcaggt ccatatttta actaaatgaa 60aattatgatt tatagccttc tcaaatacct gccatacttg atatctcaac cagagctaat 120tttacctctt tacaaattaa ataagcaagt aactggatcc acaatttata atacctgtca 180attttttctg tattaaacct ctatcatagt ttaagcctat tagggtactt aatccttaca 240aataaacagg tttaaaatca cctcaatagg caactgccct tctggttttc ttctttgact 300aaacaatctg aatgcttaag attttccact ttgggtgcta gcagtacaca gtgttacact 360ctgtattcca gacttcttaa attatagaaa aaggaatgta cactttttgt attctttctg 420agcagggccg ggaggcaaca tcatctacca tggtagggac ttgtatgcat ggactacttt 480a 481 58 141 DNA Homo sapiens 58actctgtcgc ccaggctgga gcccabtggm gcgatctcga ctccctgcaa gctmcgcctc 60acaggwtcat gccattctcc tgcctcagca tctggagtag ctgggactac aggcgccagc 120caccatgccc agctaatttt t 141 59 191 DNA Homo sapiens 59accttaaaga cataggagaa tttatactgg gagagaaagc ttacaaatgt aaggtttctg 60acaagacttg ggagtgattc acacctggaa caacatactg gacttcacac tggabagaaa 120ccttacaagt gtaatgagtg tggcaaagcc tttggcaagc agtcaacact tattcaccat 180caggcaattc a 191 60 480 DNA Homo sapiens 60agtcaggatc atgatggctc agtttcccac agcgatgaat ggagggccaa atatgtgggc 60tattacatct gaagaacgta ctaagcatga taaacagttt gataacctca aaccttcagg 120aggttacata acaggtgatc aagcccgtac ttttttccta cagtcaggtc tgccggcccc 180ggttttagct gaaatatggg ccttatcaga tctgaacaag gatgggaaga tggaccagca 240agagttctct atagctatga aactcatcaa gttaaagttg cagggccaac agctgcctgt 300agtcctccct cctatcatga aacaaccccc tatgttctct ccactaatct ctgctcgttt 360tgggatggga agcatgccca atctgtccat tcatcagcca ttgcctccag ttgcacctat 420agcaacaccc ttgtcttctg ctacttcagg gaccagtatt cctccctaat gatgcctgct 480 61 381 DNA Homo sapiens 61ctttcgattt ccttcaattt gtcacgtttg attttatgaa gttgttcaag ggctaactgc 60tgtgtattat agctttctct gagttccttc agctgattgt taaatgaatc catttctgag 120agcttagatg cagtttcttt ttcaagagca tctaattgtt ctttaagtct ttggcataat 180tcttcctttt ctgatgactt tctatgaagt aaactgatcc ctgaatcagg tgtgttactg 240agctgcatgt ttttaattct ttcgtttaat agctgcttct cagggaccag atagataagc 300ttattttgat attccttaag ctcttggtga agttgttcga tttccataat ttccaggtca 360cactggttat cccaaacttc t 381 62 906 DNA Homo sapiens 62gtggaggtga aacggaggca agaaaggggg ctacctcagg agcgagggac aaagggggcg 60tgaggcacct aggccgcggc accccggcga caggaagccg tcctgaaccg ggctaccggg 120taggggaagg gcccgcgtag tcctcgcagg gccccagagc tggagtcggc tccacagccc 180cgggccgtcg gcttctcact tcctggacct ccccggcgcc cgggcctgag gactggctcg 240gcggagggag aagaggaaac agacttgagc agctccccgt tgtctcgcaa ctccactgcc 300gaggaactct catttcttcc ctcgctcctt caccccccac ctcatgtaga aaggtgctga 360agcgtccgga gggaagaaga acctgggcta ccgtcctggc cttcccmccc ccttcccggg 420gcgctttggt gggcgtggag ttggggttgg gggggtgggt gggggttctt ttttggagtg 480ctggggaact tttttccctt cttcaggtca ggggaaaggg aatgcccaat tcagagagac 540atgggggcaa gaaggacggg agtggaggag cttctggaac tttgcagccg tcatcgggag 600gcggcagctc taacagcaga gagcgtcacc gcttggtatc gaagcacaag cggcataagt 660ccaaacactc caaagacatg gggttggtga cccccgaagc agcatccctg ggcacagtta 720tcaaaccttt ggtggagtat gatgatatca gctctgattc cgacaccttc tccgatgaca 780tggccttcaa actagaccga agggagaacg acgaacgtcg tggatcagat cggagcgacc 840gcctgcacaa acatcgtcac caccagcaca ggcgttcccg ggacttacta aaagctaaac 900agaccg 906 63 491 DNA Homo sapiens 63gacatgtttg cctgcagggg accagagaca atgggattag ccagtgctca ctgttcttta 60tgcttccaga gaggatgggg acagctctca ggtcagaatc caggctgaga aggccatgct 120ggttgggggc ccccggaagc acggtccgga tcctccctgg catcagcgta gacccgctgc 180tcaggcttgg ggtaccaaac tcatgctctg tactgttttg gccccatgcg gtgagaggaa 240aacctagaaa aagattggtc gtgctaagga atcagctgcc ccctcatcct ccgcatccaa 300tgctggtgac aacatattcc ctctcccagg acacagactc ggtgactcca cactgggctg 360agtggcctct ggaggctcgt ggcctaaggc agggctccgt aaggctgatc ggctgaactg 420ggtggggtga gggtttctga cccttcgctt cccatcccat aaccgctgtc aatgagctca 480cactgtggtc a 491 64 511 DNA Homo sapiens 64gatggcatgg tcgttgctaa tgtgcctgct gggatggagc acttcctcct gtgagcccag 60gggacccgcc tgtccctgga gcttggggca aggagggaag agtgatacca ggaaggtggg 120gctgcagcca ggggccagag tcagttcagg gagtggtcct cggccctcaa agctcctccg 180gggactgctc aggagtgatg gtgccctgga gtttgcccca acttccctgg ccaccctgga 240aggtgcctgg ctgctccagg cctctaggct gggctgatgg gtttctccag gacacaagta 300tcattaaagc caccctctcc tcagcttgtc aggccgcaca tgtgggacag gctgtgctca 360caaccccctc gcctgccctg ccctccatca ggaggagcca gtggaacctt cggaaagctc 420ccagcatctc agcagccctc aaaagtcgtc ctggggcaag ctctggttct cctgactgga 480ggtcatctgg gcttggcctg ctctctctcg c 511 65 394 DNA Homo sapiens 65taaaaaagtg taacaaaggt ttatttagac tttcttcatg cccccagatc caggatgtct 60atgtaaaccg ttatcttaca aagaaagcac aatatttggt ataaactaag tcagtgactt 120gcttaactga aatagcgtcc atccaaaagt gggtttaagg taaaactacc tgacgatatt 180ggcggggatc ctgcagtttg gactgcttgc cgggtttgtc cagggttccg ggtctgttct 240tggcactcat ggggacaggc atcctgctcg tctgtggggc cccgctggag cccttacgtg 300aagctgaagg tatcgaccst agggggctct agggcagtgg gaccttcatc cggaactaac 360aagggtcggg gagaggcctc ttgggctatg tggg 394 66 359 DNA Homo sapiens 66caagcgttcc tttatggatg taaattcaaa cagtcatgct gagccatccc gggctgacag 60tcacgttwaa gacactaggt cgggcgccac agtgccaccc aaggagaaga agaatttgga 120atttttccat gaagatgtac ggaaatctga tgttgaatat gaaaatggcc cccaaatgga 180attccaaaag gttaccacag gggctgtaag acctagtgac cctcctaagt gggaaagagg 240aatggagaat agtatttctg atgcatcaag aacatcagaa tataaaactg agatcataat 300gaaggaaaat tccatatcca atatgagttt actcagagac agtagaaact attcccagg 359 67 450 DNA Homo sapiens modified_base (425) Where n is a, c, g or t 67taggaataac aaatgtttat tcagaaatgg ataagtaata cataatcacc cttcatctct 60taatgcccct tcctctcctt ctgcacagga gacacagatg ggtaacatag aggcatggga 120agtggaggag gacacaggac tagcccacca ccttctcttc ccggtctccc aagatgactg 180cttatagagt ggaggaggca aacaggtccc ctcaatgtac cagatggtca cctatagcac 240cagctccaga tggccacgtg gttgcagctg gactcaatga aactctgtga caaccagaag 300atacctgctt tgggatgaga gggaggataa agccatgcag ggaggatatt taccatccct 360accctaagca cagtgcaagc agtgagcccc cggctcccag tacctgaaaa accaaggcct 420actgnctttt ggatgctctc ttgggccacg 450 68 511 DNA Homo sapiens 68aagcctcctg ccctggaaat ctggagcccc ttggagctga gctggacggg gcagggaggg 60gctgagaggc aagaccgtct ccctcctgct gcagctgctt ccccagcagc cactgctggg 120cacagcagaa acgccagcag agaaaatggg agccgagagt ccttagccct ggagctgagg 180ctgcctctgg gctgacccgc tggctgtacg tggccagaac tggggttggc atctggcatc 240catttgaggc cagggtggag gaaagggagg ccaacagagg aaaacctatt cctgctgtga 300caacacagcc cttgtcccac gcagcctaag tgcagggagc gtgatgaagt caggcagcca 360gtcggggagg acgaggtaac tcagcagcaa tgtcaccttg tagcctatgc gctcaatggc 420ccggaggggc agcaaccccc cgcacacgtc agccaacagc agtgcctctg caggcaccaa 480gagagcgatg atggacttga gcgccgtgtt c 511 69 511 DNA Homo sapiens 69gtttggcaga agacatgttt aataacattt tcatatttaa aaaatacagc aacaattctc 60tatctgtcca ccatcttgcc ttgcccttcc tggggctgag gcagacaaag gaaaggtaat 120gaggttaggg cccccaggcg ggctaagtgc tattggcctg ctcctgctca aagagagcca 180tagccagctg ggcacggccc cctagcccct ccaggttgct gaggcggcag cggtggtaga 240gttcttcact gagccgtggg ctgcagtctc gcagggagaa cttctgcacc agccctggct 300ctacggcccg aaagaggtgg agccctgaga accggaggaa aacatccatc acctccagcc 360cctccagggc ttcctcctct tcctggcctg ccagttcacc tgccagccgg gctcgggccg 420ccaggtagtc agcgttgtag aagcagccct ccgcagaagc ctgccggtca aatctccccg 480ctataggagc cccccgggag gggtcagcac c 511 70 511 DNA Homo sapiens 70caagttgaac gtcaggcttg gcagaggtgg agtgtagatg aaaacaaagg tgtgattatg 60aagaggatgt gagtcctttg ggtgtaggag agaaaggctg ttgagcttct atttcaagat 120acttttacct gtgcaaaaag cacattttcc acctccttct catggcattt gtgtaaggtg 180agtatgattc ctattccatc tgcattttag aggtgaagaa taacgtacaa gggattcagt 240gattagcaag ggacccctca ctaagtgttg atggagttag gacagagctc agctgtttga 300atctcagagc ccaggcagct ggagctgggt aggatcctgg agctggcact aatgtgaggt 360gcattccctc caacccaggc tcagatccgg aacctgaccg tgctgacccc cgaaggggag 420gcagggctga gctggcccgt tgggctccct gctcctttca caccacactc tcgctttgag 480gtgctgggct gggactactt cacagagcag c 511 71 511 DNA Homo sapiens 71tggcctgggc aggattggga gagaggtagc tacccggatg cagtcctttg ggatgaagac 60tatagggtat gaccccatca tttccccaga ggtctcggcc tcctttggtg ttcagcagct 120gcccctggag gagatctggc ctctctgtga tttcatcact gtgcacactc ctctcctgcc 180ctccacgaca ggcttgctga atgacaacac ctttgcccag tgcaagaagg gggtgcgtgt 240ggtgaactgt gcccgtggag ggatcgtgga cgaaggcgcc ctgctccggg ccctgcagtc 300tggccagtgt gccggggctg cactggacgt gtttacggaa gagccgccac gggaccgggc 360cttggtggac catgagaatg tcatcagctg tccccacctg ggtgccagca ccaaggaggc 420tcagagccgc tgtggggagg aaattgctgt tcagttcgtg gacatggtga aggggaaatc 480tctcacgggg gttgtgaatg cccaggccct t 511 72 2017 DNA Homo sapiens 72agccagatgg ctgagagctg caagaagaag tcaggatcat gatggctcag tttcccacag 60cgatgaatgg agggccaaat atgtgggcta ttacatctga agaacgtact aagcatgata 120aacagtttga taacctcaaa ccttcaggag gttacataac aggtgatcaa gcccgtactt 180ttttcctaca gtcaggtctg ccggccccgg ttttagctga aatatgggcc ttatcagatc 240tgaacaagga tgggaagatg gaccagcaag agttctctat agctatgaaa ctcatcaagt 300taaagttgca gggccaacag ctgcctgtag tcctccctcc tatcatgaaa caacccccta 360tgttctctcc actaatctct gctcgttttg ggatgggaag catgcccaat ctgtccattc 420atcagccatt gcctccagtt gcacctatag caacaccctt gtcttctgct acttcaggga 480ccagtattcc tcccctaatg atgcctgctc ccctagtgcc ttctgttagt acatcctcat 540taccaaatgg aactgccagt ctcattcagc ctttatccat tccttattct tcttcaacat 600tgcctcatgc atcatcttac agcctgatga tgggaggatt tggtggtgct agtatccaga 660aggcccagtc tctgattgat ttaggatcta gtagctcaac ttcctcaact gcttccctct 720cagggaactc acctaagaca gggacctcag agtgggcagt tcctcagcct tcaagattaa 780agtatcggca aaaatttaat agtctagaca aaggcatgag cggatacctc tcaggttttc 840aagctagaaa tgcccttctt cagtcaaatc tctctcaaac tcagctagct actatttgga 900ctctggctga catcgatggt gacggacagt tgaaagctga agaatttatt ctggcgatgc 960acctcactga catggccaaa gctggacagc cactaccact gacgttgcct cccgagcttg 1020tccctccatc tttcagaggg ggaaagcaag ttgattctgt taatggaact ctgccttcat 1080atcagaaaac acaagaagaa gagcctcaga agaaactgcc agttactttt gaggacaaac 1140ggaaagccaa ctatgaacga ggaaacatgg agctggagaa gcgacgccaa gtgttgatgg 1200agcagcagca gagggaggct gaacgcaaag cccagaaaga gaaggaagag tgggagcgga 1260aacagagaga actgcaagag caagaatgga agaagcagct ggagttggag aaacgcttgg 1320agaaacagag agagctggag agacagcggg aggaagagag gagaaaggag atagaaagac 1380gagaggcagc aaaacaggag cttgagagac aacgccgttt agaatgggaa agactccgtc 1440ggcaggagct gctcagtcag aagaccaggg aacaagaaga cattgtcagg ctgagctcca 1500gaaagaaaag tctccacctg gaactggaag cagtgaatgg aaaacatcag cagatctcag 1560gcagactaca agatgtccaa atcagaaagc aaacacaaaa gactgagcta gaagttttgg 1620ataaacagtg tgacctggaa attatggaaa tcaaacaact tcaacaagag cttaaggaat 1680atcaaaataa gcttatctat ctggtccctg agaagcagct attaaacgaa agaattaaaa 1740acatgcagct cagtaacaca cctgattcag ggatcagttt acttcataaa aagtcatcag 1800aaaaggaaga attatgccaa agacttaaag aacaattaga tgctcttgaa aaagaaactg 1860catctaagct ctcagaaatg gattcattta acaatcagct gaaggaactc agagaaagct 1920ataatacaca gcagttagcc cttgaacaac ttcataaaat caaacgtgac aaattgaagg 1980aaatcgaaag aaaaagatta gagcaaaaaa aaaaaaa 2017 73 414 DNA Homo sapiens 73atggcagtga cattcaccat catgggaacc accttccctt ttcttcagga ttctctgtag 60tggaagagag cacccagtgt tgggctgaaa acatctgaaa gtagggagaa gaacctaaaa 120taatcagtat ctcagagggc tctaaggtgc caagaagtct cactggacat ttaagtgcca 180acaaaggcat actttcggaa tcgccaagtc aaaactttct aacttctgtc tctctcagag 240acaagtgaga ctcaagagtc tactgcttta gtggcaacta cagaaaactg gtgttaccca 300gaaaaacagg agcaattaga aatggttcca atatttcaaa gctccgcaaa caggatgtgc 360tttcctttgc ccatttaggg tttcttctct ttcctttctc tttattaacc acta 414 74 1567 DNA Homo sapiens 74atatctagaa gtctggagtg agcaaacaag agcaagaaac aaaaagaagc caaaagcaga 60aggctccaat atgaacaaga taaatctatc ttcaaagaca tattagaagt tgggaaaata 120attcatgtga actagacaag tgtgttaaga gtgataagta aaatgcacgt ggagacaagt 180gcatccccag atctcaggga cctccccctg cctgtcacct ggggagtgag aggacaggat 240agtgcatgtt ctttgtctct gaatttttag ttatatgtgc tgtaatgttg ctctgaggaa 300gcccctggaa agtctatccc aacatatcca catcttatat tccacaaatt aagctgtagt 360atgtacccta agacgctgct aattgactgc cacttcgcaa ctcaggggcg gctgcatttt 420agtaatgggt caaatgattc actttttatg atgcttccaa aggtgccttg gcttctcttc 480ccaactgaca aatgccaaag ttgagaaaaa tgatcataat tttagcataa acagagcagt 540cggcgacacc gattttataa ataaactgag caccttcttt ttaaacaaac aaatgcgggt 600ttatttctca gatgatgttc atccgtgaat ggtccaggga aggacctttc accttgacta 660tatggcatta tgtcatcaca agctctgagg cttctccttt ccatcctgcg tggacagcta 720agacctcagt tttcaatagc atctagagca gtgggactca gctggggtga tttcgccccc 780catctccggg ggaatgtctg aagacaattt tgttacctca atgagggagt ggaggaggat 840acagtgctac taccaactag tggataaagg ccagggatgc tgctcaacct cctaccatgt 900acaggacgtc tccccattac aactacccaa tccgaagtgt caactgtgtc aggactaaga 960aaccctggtt ttgagtagaa aagggcctgg aaagagggga gccaacaaat ctgtctgctt 1020cctcacatta gtcattggca aataagcatt ctgtctcttt ggctgctgcc tcagcacaga 1080gagccagaac tctatcgggc accaggataa catctctcag tgaacagagt tgacaaggcc 1140tatgggaaat gcctgatggg attatcttca gcttgttgag cttctaagtt tctttccctt 1200cattctaccc tgcaagccaa gttctgtaag agaaatgcct gagttctagc tcaggttttc 1260ttactctgaa tttagatctc cagacccttc ctggccacaa ttcaaattaa ggcaacaaac 1320atataccttc catgaagcac acacagactt ttgaaagcaa ggacaatgac tgcttgaatt 1380gaggccttga ggaatgaagc tttgaaggaa aagaatactt tgtttccagc ccccttccca 1440cactcttcat gtgttaacca ctgccttcct ggaccttgga gccacggtga ctgtattaca 1500tgttgttata gaaaactgat tttagagttc tgatcgttca agagaatgat taaatataca 1560tttccta 1567 75 240 DNA Homo sapiens 75tcgagcggcc gcccgggcag gtccttcaga cttggactgt gtcacactgc caggcttcca 60gggctccaac ttgcagacgg cctgttgtgg gacagtctct gtaatcgcga aagcaaccat 120ggaagacctg ggggaaaaca ccatggtttt atccaccctg agatctttga acaacttcat 180ctctcagcgt gcggagggag gctctggact ggatatttct acctcggccg cgaccacgct 240 76 330 DNA Homo sapiens modified_base (288) Where n is a, c, g or t 76tagcgyggtc gcggccgagg yctgcttytc tgtccagccc agggcctgtg gggtcagggc 60ggtgggtgca gatggcatcc actccggtgg cttccccatc tttctctggc ctgagcaagg 120tcagcctgca gccagagtac agagggccaa cactggtgtt cttgaacaag ggccttagca 180ggccctgaag grccctctct gtagtgttga acttcctgga gccaggccac atgttctcct 240cataccgcag gytagygatg gtgaagttga gggtgaaata gtattmangr agatggctgg 300caracctgcc cgggcggccg ctcsaaatcc 330 77 361 DNA Homo sapiens 77agcgtggtcg cggccgaggt gtccttcagg gtctgcttat gcccttgttc aagaacacca 60gtgtcagctc tctgtactct ggttgcagac tgaccttgct caggcctgag aaggatgggg 120cagccaccag agtggatgct gtctgcaccc atcgtcctga ccccaaaagc cctggactgg 180acagagagcg gctgtactgg aagctgagcc agctgaccca cggcatcact gagctgggcc 240cctacaccct ggacagggac agtctctatg tcaatggttt cacccatcgg agctctgtac 300ccaccaccag caccggggtg gtcagcgagg agccattcaa cctgcccggg cggccgctcg 360a 361 78 356 DNA Homo sapiens modified_base (7) Where n is a, c, g or t 78ttggggnttt mgagcggccg cccgggcagg taccggggtg gtcagcgagg agccattcac 60actgaacttc accatcaaca acctgcggta tgaggagaac atgcagcacc ctggctccag 120gaagttcaac accacggaga gggtccttca gggcctgctc aggtccctgt tcaagagcac 180cagtgttggc cctctgtact ctggctgcag actgactttg ctcagacttg agaaacatgg 240ggcagccact ggagtggacg ccatctgcac cctccgcctt gatcccactg gtcctggact 300ggacagagag cggctatact gggagctgag ccagtcctct ggcggngacn ccnctt 356 79 226 DNA Homo sapiens 79agcgtggtcg cggccgaggt ccagtcgcag catgctcttt ctcctgccca ctggcacagt 60gaggaagatc tctgctgtca gtgagaaggc tgtcatccac tgagatggca gtcaaaagtg 120catttaatac acctaacgta tcgaacatca tagcttggcc caggttatct catatgtgct 180cagaacactt acaatagcct gcagacctgc ccgggcggcc gctcga 226 80 444 DNA Homo sapiens modified_base (23) Where n is a, c, g or t 80tgtggtgttg aacttcctgg agncagggtg acccatgtcc tccccatact gcaggttggt 60gatggtgaag ttgagggtga atggtaccag gagagggcca gcagccataa ttgtsgrgck 120gsmgmssgag gmwggwgtyy cwgaggttcy rarrtccact gtggaggtcc caggagtgct 180ggtggtgggc acagagstcy gatgggtgaa accattgaca tagagactgt tcctgtccag 240ggtgtagggg cccagctctt yratgycatt ggycagttkg ctyagctccc agtacagccr 300ctctckgyyg mgwccagsgc ttttggggtc aagatgatgg atgcagatgg catccactcc 360agtggctgct ccatccttct cggacctgag agaggtcagt ctgcagccag agtacagagg 420gccaacactg gtgttctttg aata 444 81 310 DNA Homo sapiens 81tcgagcggcc gcccgggcag gtcaggaagc acattggtct tagagccact gcctcctgga 60ttccacctgt gctgcggaca tctccaggga gtgcagaagg gaagcaggtc aaactgctca 120gatcagtcag actggctgtt ctcagttctc acctgagcaa ggtcagtctg cagccagagt 180acagagggcc aacactggtg ttcttgaaca agggcttgag cagaccctgc agaaccctct 240tccgtggtgt tgaacttcct ggaaaccagg gtgttgcatg tttttcctca taatgcaagg 300ttggtgatgg 310 82 571 DNA Homo sapiens modified_base (202) Where n is a, c, g or t 82acggtttcaa tggacacttt tattgtttac ttaatggatc atcaattttg tctcactacc 60tacaaatgga atttcatctt gtttccatgc tgagtagtga aacagtgaca aagctaatca 120taataaccta catcaaaaga gaactaagct aacactgctc actttctttt taacaggcaa 180aatataaata tatgcactct anaatgcaca atggtttagt cactaaaaaa ttcaaatggg 240atcttgaaga atgtatgcaa atccagggtg cagtgaagat gagctgagat gctgtgcaac 300tgtttaaggg ttcctggcac tgcatctctt ggccactagc tgaatcttga catggaaggt 360tttagctaat gccaagtgga gatgcagaaa atgctaagtt gacttagggg ctgtgcacag 420gaactaaaag gcaggaaagt actaaatatt gctgagagca tccaccccag gaaggacttt 480accttccagg agctccaaac tggcaccacc cccagtgctc acatggctga ctttatcctc 540cgtgttccat ttggcacagc aagtggcagt g 571 83 551 DNA Homo sapiens 83aaggctggtg ggtttttgat cctgctggag aacctccgct ttcatgtgga ggaagaaggg 60aagggaaaag atgcttctgg gaacaaggtt aaagccgagc cagccaaaat agaagctttc 120cgagcttcac tttccaagct aggggatgtc tatgtcaatg atgcttttgg cactgctcac 180agagcccaca gctccatggt aggagtcaat ctgccacaga aggctggtgg gtttttgatg 240aagaaggagc tgaactactt tgcaaaggcc ttggagagcc cagagcgacc cttcctggcc 300atcctgggcg gagctaaagt tgcagacaag atccagctca tcaataatat gctggacaaa 360gtcaatgaga tgattattgg tggtggaatg gcttttacct tccttaaggt gctcaacaac 420atggagattg gcacttctct gtttgatgaa gagggagcca agattgtcaa agacctaatg 480tccaaagctg agaagaatgg tgtgaagatt accttgcctg ttgactttgt cactgctgac 540aagtttgatg a 551 84 571 DNA Homo sapiens 84tttgttcctt acatttttct aaagagttac ttaaatcagt caactggtct ttgagactct 60taagttctga ttccaactta gctaattcat tctgagaact gtggtatagg tggcgtgtct 120cttctagctg ggacaaaagt tctttgtttt ccccctgtag agtatcacag accttctgct 180gaagctggac ctctgtctgg gccttggact cccaaatctg cttgtcatgt tcaagcctgg 240aaatgttaat ctttaattct tccatatgga tggacatctg tctaagttga tcctttagaa 300cactgcaatt atcttctttg agtctaattt cttcttcttt gctttgaatc gcatcactaa 360acttcctctc ccatttctta gcttcatcta tcaccctgtc acgatcatcc tggagggaag 420acatgctctt agtaaaggct gcaagctggg tcacagtact gtccaagttt tcctgaagtt 480gctgaacttc cttgtctttc ttgttcaaag taacctgaat ctctccaatt gtctcttcca 540agtggacttt ttctctgcgc aaagcatcca g 571 85 561 DNA Homo sapiens 85tcattgcctg tgatggcatc tggaatgtga tgagcagcca ggaagttgta gatttcattc 60aatcaaagga ttcagcatgt ggtggaagct gtgaggcaag agaaacaaga actgtatggc 120aagttaagaa gcacagaggc aaacaagaag gagacagaaa agcagttgca ggaagctgag 180caagaaatgg aggaaatgaa agaaaagatg agaaagtttg ctaaatctaa acagcagaaa 240atcctagagc tggaagaaga gaatgaccgg cttagggcag aggtgcaccc tgcaggagat 300acagctaaag agtgtatgga aacacttctt tcttccaatg ccagcatgaa ggaagaactt 360gaaagggtca aaatggagta tgaaaccctt tctaagaagt ttcagtcttt aatgtctgag 420aaagactctc taagtgaaga ggttcaagat ttaaagcatc agatagaagg taatgtatct 480aaacaagcta acctagaggc caccgagaaa catgataacc aaacgaatgt cactgaagag 540ggaacacagt ctataccagg t 561 86 795 DNA Homo sapiens 86aagccaataa tcaccattta ttacttaata tatgccaacc actgtacttg gcagttcaca 60aattctcacc gttacaacaa ccccatgagg tatttattcc cattctatag atagggaaac 120cacagctcaa gtaagttagg aaactgagcc aagtatacac agaatacgaa gtggcaaaac 180tagaaggaaa gactgacact gctatctgct ggcctccagt gtcctggctc ttttcacacg 240ggttcaatgt ctccagcgct gctgctgctg ctgcattacc atgccctcat tgtttttctt 300cctctggtgt tcaactgcat ccttcaaaga atctaactca ttccagagac cacttatttc 360tttctctctt tctgaaatta cttttaataa ttcttcatga gggggaaaag aagatgcctg 420ttggtagttt tgttgtttaa gctgctcaat ttgggactta aacaatttgt tttcatcttg 480tacatcctgt aacagctgtg ttttgctaga aagatcactc tccctctctt ttagcatggc 540ttctaacctc ttcaattcat tttccttttc tttcaacaca atctcaagtt cttcaaactg 600tgatgcagaa gaggcctctt tcaagttatg ttgtgctact tcctgaacat gtgcttttaa 660agattcattt tcttcttgaa gatcctgtaa ccacttccct gtattggcta ggtctttctc 720tttctcttcc aaaacagcct tcatggtatt catctgttcc tcttttcctt ttaataagtt 780caggagcttc agaac 795 87 594 DNA Homo sapiens 87caagcttttt tttttttttt aaaaagtgtt agcattaatg ttttattgtc acgcagatgg 60caactgggtt tatgtcttca tattttatat ttttgtaaat taaaaaaatt acaagtttta 120aatagccaat ggctggttat attttcagaa aacatgatta gactaattca ttaatggtgg 180cttcaagctt ttccttattg gctccagaaa attcacccac cttttgtccc ttcttaaaaa 240actggaatgt tggcatgcat ttgacttcac actctgaagc aacatcctga cagtcatcca 300catctacttc aaggaatatc acgttggaat acttttcaga gagggaatga aagaaaggct 360tgatcatttt gcaaggccca caccacgtgg ctgagaagtc aactactaca agtttatcac 420ctgcagcgtc caaggcttcc tgaaaagcag tcttgctctc gatctgcttc accatcttgg 480ctgctggagt ctgacgagcg gctgtaagga ccgatggaaa tggatccaaa gcaccaaaca 540gagcttcaag actcgctgct tggcttgaat tcggatccga tatcgccatg gcct 594 88 557 DNA Homo sapiens 88aagtgttagc attaatgttt tattgtcacg cagatggcaa ctgggtttat gtcttcatat 60tttatatttt tgtaaattaa aaaaattmca agttttaaat agccaatggc tggttatatt 120ttcagaaaac atgattagac taattcatta atggtggctt caagcttttc cttattggct 180ccagaaaatt cacccacctt ttgtcccttc ttaaaaaact ggaatgttgg catgcatttg 240acttcacact ctgaagcaac atcctgacag tcatccacat ctacttcaag gaatatcacg 300ttggaatact tttcagagag ggaatgaaag aaaggcttga tcattttgca aggcccacac 360cacgtggctg agaagtcaac tactacaagt ttatcacctg cagcgtccaa ggcttcctga 420aaagcagtct tgctctcgat ctgcttcacc atcttggctg ctggagtctg acgagcggct 480gtaaggaccg atggaaatgg atccaaagca ccaaacagag cttcaagact cgctgcttgg 540catgaattcg gatccga 557 89 561 DNA Homo sapiens modified_base (544) Where n is a, c, g or t 89tacaaacttt attgaaacgc acacgcgcac acacacaaac acccctgtgg atagggaaaa 60gcacctggcc acagggtcca ctgaaacggg gaggggatgg cagcttgtaa tgtggctttt 120gccacaaccc ccttctgaca gggaaggcct tagattgagg ccccacctcc catggtgatg 180gggagctcag aatggggtcc agggagaatt tggttagggg gaggtgctag ggaggcatga 240gcagagggca ccctccgagt ggggtcccga gggctgcaga gtcttcagta ctgtccctca 300cagcagctgt ctcaaggctg ggtccctcaa aggggcgtcc cagcgcgggg cctccctgcg 360caaacacttg gtacccctgg ctgcgcagcg gaagccagca ggacagcagt ggcgccgatc 420agcacaacag acgccctggc ggtagggaca gcaggcccag ccctgtcggt tgtctcggca 480gcaggtctgg ttatcatggc agaagtgtcc ttcccacact tcacgtcctt cacacccacg 540tganggctac nggccaggaa g 561 90 561 DNA Homo sapiens 90cccgtgggtg ccatccacgg agttgttacc tgatctttgg aagcaggatc gcccgtctgc 60actgcagtgg aagccccgtg ggcagcagtg atggccatcc ccgcatgcca cggcctctgg 120gaaggggcag caactggaag tccctgagac ggtaaagatg caggagtggc cggcagagca 180gtgggcatca acctggcagg ggccacccag atgcctgctc agtgttgtgg gccatttgtc 240cagaagggga cggcagcagc tgtagctggc tcctccgggg tccaggcagc aggccacagg 300gcagaactga ccatctgggc accgcgttcc agccaccagc cctgctgtta aggccaccca 360gctcaccagg gtccacatgg tctgcctgcg tccgactccg cggtccttgg gccctgatgg 420ttctacctgc tgtgagctgc ccagtgggaa gtatggctgc tgccaatgcc caacgccacc 480tgctgctccg atcacctgca ctgctgcccc aagacactgt gtgtgacctg atccagagta 540agtgcctctc caaggagaac g 561 91 541 DNA Homo sapiens modified_base (480) Where n is a, c, g or t 91gaatcacctt tctggtttag ctagtacttt gtacagaaca atgaggtttc ccacagcgga 60gtctccctgg gctctgtttg gctctcggta aggcaggcct acaccttttc ctctcctcta 120tggagagggg aatatgcatt aaggtgaaaa gtcaccttcc aaaagtgaga aagggattcg 180attgctgctt caggactgtg gaattatttg gaatgtttta caaatggttg ctacaaaaca 240acaaaaaagg taattacaaa atgtgtacat cacaacatgc tttttaaaga cattatgcat 300tgtgctcaca ttcccttaaa tgttgtttcc aaaggtgctc agcctctagc ccagctggat 360tctccgggaa gaggcagaga cagtttggcg aaaaagacac agggaaggag ggggtggtga 420aaggagaaag cagccttcca gttaaagatc agccctcagt taaaggtcag cttcccgcan 480gctggcctca ngcggagtct gggtcagagg gaggagcagc agcagggtgg gactggggcg 540t 541 92 551 DNA Homo sapiens 92aaccggagcg cgagcagtag ctgggtgggc accatggctg ggatcaccac catcgaggcg 60gtgaagcgca agatccaggt tctgcagcag caggcagatg atgcagagga gcgagctgag 120cgcctccagc gagaagttga gggagaaagg cgggcccggg aacaggctga ggctgaggtg 180gcctccttga accgtaggat ccagctggtt gaagaagagc tggaccgtgc tcaggagcgc 240ctggccactg ccctgcaaaa gctggaagaa gctgaaaaag ctgctgatga gagtgagaga 300ggtatgaagg ttattgaaaa ccgggcctta aaagatgaag aaaagatgga actccaggaa 360atccaactca aagaagctaa gcacattgca gaagaggcag ataggaagta tgaagaggtg 420gctcgtaagt tggtgatcat tgaaggagac ttggaacgca cagaggaacg agctgagctg 480gcagagtccc gttgccgaga gatggatgag cagattagac tgatggacca gaacctgaag 540tgtctgagtg c 551 93 531 DNA Homo sapiens 93gagaacttgg cctttattgt gggcccagga gggcacaaag gtcaggaggc ccaagggagg 60gatctggttt tctggatagc caggtcatag catgggtatc agtaggaatc cgctgtagct 120gcacaggcct cacttgctgc agttccgggg agaacacctg cactgcatgg cgttgatgac 180ctcgtggtac acgacagagc cattggtgca gtgcaagggc acgcgcatgg gctccgtcct 240cgagggcagg cagcaggagc attgctcctg cacatcctcg atgtcaatgg agtacacagc 300tttgctggca cactttccct ggcagtaatg aatgtccact tcctcttggg acttacaatc 360tcccactttg atgtactgca ccttggctgt gatgtctttg caatcaggct cctcacatgt 420gtcacagcag gtgcctggaa ttttcacgat tttgcctcct tcagccagac acttgtgttc 480atcaaatggt gggcagcccg tgaccctctt ctcccagatg tactctcctc t 531 94 531 DNA Homo sapiens modified_base (517) Where n is a, c, g or t 94gcctggacct tgccggatca gtgccacaca gtgacttgct tggcaaatgg ccagaccttg 60ctgcagagtc atcgtgtcaa ttgtgaccat ggaccccggc cttcatgtgc caacagccag 120tctcctgttc gggtggagga gacgtgtggc tgccgctgga cctgcccttg tgtgtgcacg 180ggcagttcca ctcggcacat cgtcaccttc gatgggcaga atttcaagct tactggtagc 240tgctcctatg tcatctttca aaacaaggag caggacctgg aagtgctcct ccacaatggg 300gcctgcagcc ccggggcaaa acaagcctgc atgaagtcca ttgagattaa gcatgctggc 360gtctctgctg agctgcacag taacatggag atggcagtgg atgggagact ggtccttgcc 420ccgtacgttg gtgaaaacat ggaagtcagc atctacggcg ctatcatgta tgaagtcagg 480tttacccatc ttggccacat cctcacatac accgccncaa aacaacgagt t 531 95 605 DNA Homo sapiens 95agatcaacct ctgctggtca ggaggaatgc cttccttgtc ttggatcttt gctttgacgt 60tctcgatagt rwcaactkkr ytsramskma agkgyratgr wmttksywgw rasyktmwwm 120rsgraraytt agacaycccm cctcwgagac gsagkaccar gtgcagaggt ggactctttc 180tggatgttgt agtcagacag ggtgcgtcca tcttccagct gtttcccagc aaagatcaac 240ctctgctgat caggagggat gccttcctta tcttggatct ttgccttgac attctcgatg 300gtgtcactgg gctccacctc gagggtgatg gtcttaccag tcagggtctt cacgaagaty 360tgcatcccac ctctgagacg gagcaccagg tgcagggtrg actctttctg gatgttgtag 420tcagacaggg tgcgyccatc ttccagctgc tttccsagca aagatcaacc tctgctggtc 480aggaggratg ccttccttgt cytggatctt tgcyttgacr ttctcratgg tgtcactcgg 540ctccacttcg agagtgatgg tcttaccagt cagggtcttc acgaagatct gcatcccacc 600tctaa 605 96 531 DNA Homo sapiens 96aagtcacaaa cagacaaaga ttattaccag ctgcaagcta tattagaagc tgaacgaaga 60gacagaggtc atgattctga gatgattgga gaccttcaag ctcgaattac atctttacaa 120gaggaggtga agcatctcaa acataatctc gaaaaagtgg aaggagaaag aaaagaggct 180caagacatgc ttaatcactc agaaaaggaa aagaataatt tagagataga tttaaactac 240aaacttaaat cattacaaca acggttagaa caagaggtaa atgaacacaa agtaaccaaa 300gctcgtttaa ctgacaaaca tcaatctatt gaagaggcaa agtctgtggc aatgtgtgag 360atggaaaaaa agctgaaaga agaaagagaa gctcgagaga aggctgaaaa tcgggttgtt 420cagattgaga aacagtgttc catgctagac gttgatctga agcaatctca gcagaaacta 480gaacatttga ctggaaataa agaaaggatg gaggatgaag ttaagaatct a 531 97 1017 DNA Homo sapiens modified_base (963) Where n is a, c, g or t 97cgcctccacc atgtccatca gggtgaccca gaagtcctac aaggtgtcca cctctggccc 60ccgggccttc agcagccgct cctacacgag tgggcccggt tcccgcatca gctcctcgag 120cttctcccga gtgggcagca gcaactttcg cggtggcctg ggcggcggct atggtggggc 180cagcggcatg ggaggcatca ccgcagttac ggtcaaccag agcctgctga gcccccttgt 240cctggaggtg gaccccaaca tccaggccgt gcgcacccag gagaaggagc agatcaagac 300cctcaacaac aagtttgcct ccttcataga caaggtacgg ttcctggagc agcagaacaa 360gatgctggag accaagtgga gcctcctgca gcagcagaag acggctcgaa gcaacatgga 420caacatgttc gagagctaca tcaacarcct taggcggcag ctggagactc tgggccagga 480gaagctgaag ctggaggcgg agcttggcaa catgcagggg ctggtggagg acttcaagaa 540caagtatgag gatgagatca ataagcgtac agagatggag aacgaatttg tcctcatcaa 600gaaggatgtg gatgaagctt acatgaacaa ggtagagctg gagtctcgcc tggaagggct 660gaccgacgag atcaacttcc tcaggcagct gtatgaagag gagatccggg agctgcagtc 720ccagatctcg gacacatctg tggtgctgtc catggacaac agccgctccc tggacatgga 780cagcatcatt gctgaggtca aggcacagta cgaggatatt gccaaccgca gccgggctga 840ggctgagagc atgtaccagg tcaagtatga ggagctgcag agcctggctg ggaagcacgg 900ggatgacctg cggcgcacaa agactgagat ctctgagatg aacccggaac atcagcccgg 960ctncaggctg agattgaggg cctcaaaggc caganggctt ncctggangn ccgccat 1017 98 561 DNA Homo sapiens 98cccggagcca gccaacgagc ggaaaatggc agacaatttt tcgctccatg atgcgttatc 60tgggtctgga aacccaaacc ctcaaggatg gcctggcgca tgggggaacc agcctgctgg 120ggcagggggc tacccagggg cttcctatcc tggggcctac cccgggcagg cacccccagg 180ggcttatcct ggacaggcac ctccaggcgc ctaccctgga gcacctggag cttatcccgg 240agcacctgca cctggagtct acccagggcc acccagcggc cctggggcct acccatcttc 300tggacagcca agtgccaccg gagcctaccc tgccactggc ccctatggcg cccctgctgg 360gccactgatt gtgccttata acctgccttt gcctggggga gtggtgcctc gcatgctgat 420aacaattctg ggcacggtga agcccaatgc aaacagaatt gctttagatt tccaaagagg 480gaatgatgtt gccttccact ttaacccacg cttcaatgag aacaacagga gagtcattgg 540ttgcaataca aagctggata a 561 99 636 DNA Homo sapiens 99gggaatgcaa caactttatt gaaaggaaag tgcaatgaaa tttgttgaaa ccttaaaagg 60ggaaacttag acaccccccc tcragcgmag kaccargtgc araggtggac tctttctgga 120tgttgtagtc agacagggtr cgwccatctt ccagctgttt yccrgcaaag atcaacctct 180gctgatcagg aggratgcct tccttatctt ggatctttgc cttgacattc tcgatggtgt 240cactgggctc cacctcgagg gtgatggtct taccagtcag ggtcttcacg aagatytgca 300tcccacctct gagacggagc accaggtgca gggtrgactc tttctggatg ttgtagtcag 360acagggtgcg yccatcttcc agctgctttc csagcaaaga tcaacctctg ctggtcagga 420ggratgcctt ccttgtcytg gatctttgcy ttgacrttct caatggtgtc actcggctcc 480acttcgagag tgatggtctt accagtcagg gtcttcacga agatctgcat cccacctcta 540agacggagca ccaggtgcag ggtggactct ttctggatgg ttgtagtcag acagggtgcg 600tccatcttcc agctgtttcc cagcaaagat caacct 636 100 697 DNA Homo sapiens 100aggttgatct ttgctgggaa acagctggaa gatggacgca ccctgtctga ctacaaccat 60ccagaaagag tccaccctgc acctggtgct ccgtcttaga ggtgggatgc agatcttcgt 120gaagaccctg actggtaaga ccatcactct cgaagtggag ccgagtgaca ccattgagaa 180ygtcaargca aagatccarg acaaggaagg catycctcct gaccagcaga ggttgatctt 240tgctsggaaa gcagctggaa gatggrcgca ccctgtctga ctacaacatc cagaaagagt 300cyaccctgca cctggtgctc cgtctcagag gtgggatgca ratcttcgtg aagaccctga 360ctggtaagac catcaccctc gaggtggagc ccagtgacac catcgagaat gtcaaggcaa 420agatccaaga taaggaaggc atccctcctg atcagcagag gttgatcttt gctgggaaac 480agctggaaga tggacgcacc ctgtctgact acaacatcca gaaagagtcc acctytgcac 540ytggtmctbc gtctyagagg kgggrtgcaa atctwmgtkw agacactcac tkkyaagryy 600atcamcmwtg akktcgakys castkwcact wtcrakaamg tyrwwgcawa gatccmagac 660aaggaaggca ttcctcctga ccagcagagg ttgatct 697 101 451 DNA Homo sapiens 101atggagtctc actctgtcga ccaggctgga gcgctgtggt gcgatatcgg ctcactgcag 60tctccacttc ctgggttcaa gcgatcctcc tgcctcagcc tcccgagtag ctgggactac 120aggcaggcgt caccataatt tttgtatttt tagtagagac atggtttcgc catgttggct 180gggctggtct cgaactcctg acctcaagtg atctgtcctg gcctcccaaa gtgttgggat 240tacaggcgaa agccaacgct cccggccagg gaacaacttt agaatgaagg aaatatgcaa 300aagaacatca catcaaggat caattaatta ccatctatta attactatat gtgggtaatt 360atgactattt cccaagcatt ctacgttgac tgcttgagaa gatgtttgtc ctgcatggtg 420gagagtggag aagggccagg attcttaggt t 451 102 571 DNA Homo sapiens 102agcgcggtct tccggcgcga gaaagctgaa ggtgatgtgg ccgccctcaa ccgacgcatc 60cagctcgttg aggaggagtt ggacagggct caggaacgac tggccacggc cctgcagaag 120ctggaggagg cagaaaaagc tgcagatgag agtgagagag gaatgaaggt gatagaaaac 180cgggccatga aggatgagga gaagatggag attcaggaga tgcagctcaa agaggccaag 240cacattgcgg aagaggctga ccgcaaatac gaggaggtag ctcgtaagct ggtcatcctg 300gagggtgagc tggagagggc agaggagcgt gcggaggtgt ctgaactaaa atgtggtgac 360ctggaagaag aactcaagaa tgttactaac aatctgaaat ctctggaggc tgcatctgaa 420aagtattctg aaaaggagga caaatatgaa gaagaaatta aacttctgtc tgacaaactg 480aaagaggctg agacccgtgc tgaatttgca gagagaacgg ttgcaaaact ggaaaagaca 540attgatgacc tggaagagaa acttgcccag c 571 103 451 DNA Homo sapiens 103gtgcacaggt cccatttatt gtagaaaata ataataatta cagtgatgaa tagctcttct 60taaattacaa aacagaaacc acaaagaagg aagaggaaaa accccaggac ttccaagggt 120gaagctgtcc cctcctccct gccaccctcc caggctcatt agtgtccttg gaaggggcag 180aggactcaga ggggatcagt ctccaggggc cctgggctga agcgggtgag gcagagagtc 240ctgaggccac agagctgggc aacctgagcc gcctctctgg ccccctcccc caccactgcc 300caaacctgtt tacagcacct tcgcccctcc cctctaaacc cgtccatcca ctctgcactt 360cccaggcagg tgggtgggcc aggcctcagc catactcctg ggcgcgggtt tcggtgagca 420aggcacagtc ccagaggtga tatcaaggcc t 451 104 441 DNA Homo sapiens 104gcaaggaact ggtctgctca cacttgctgg cttgcgcatc aggactggct ttatctcctg 60actcacggtg caaaggtgca ctctgcgaac gttaagtccg tccccagcgc ttggaatcct 120acggccccca cagccggatc ccctcagcct tccaggtcct caactcccgt ggacgctgaa 180caatggcctc catggggcta caggtaatgg gcatcgcgct ggccgtcctg ggctggctgg 240ccgtcatgct gtgctgcgcg ctgcccatgt ggcgcgtgac ggccttcatc ggcagcaaca 300ttgtcacctc gcagaccatc tgggagggcc tatggatgaa ctgcgtggtg cagagcaccg 360gccagatgca gtgcaaggtg tacgactcgc tgctggcact gccgcaggac ctgcaggcgg 420cccgcgccct cgtcatcatc a 441 105 509 DNA Homo sapiens modified_base (195) Where n is a, c, g or t 105tgcaaaaggg acacaggggt tcaaaaataa aaatttctct tccccctccc caaacctgta 60ccccagctcc ccgaccacaa cccccttcct cccccgggga aagcaagaag gagcaggtgt 120ggcatctgca gctgggaaga gagaggccgg ggaggtgccg agctcggtgc tggtctcttt 180ccaaatataa atacntgtgt cagaactgga aaatcctcca gcacccacca cccaagcact 240ctccgttttc tgccggtgtt tggagagggg cggggggcag gggcgccagg caccggctgg 300ctgcggtcta ctgcatccgc tgggtgtgca ccccgcgagc ctcctgctgc tcattgtaga 360agagatgaca ctcggggtcc ccccggatgg tgggggctcc ctggatcagc ttcccggtgt 420tggggttcac acaccagcac tccccacgct gcccgttcag agacatcttg cactgtttga 480ggttgtacag gccatgcttg tcacagttg 509 106 571 DNA Homo sapiens 106gggttggagg gactggttct ttatttcaaa aagacacttg tcaatattca gtatcaaaac 60agttgcacta ttgatttctc tttctcccaa tcggccccaa agagaccaca taaaaggaga 120gtacatttta agccaataag ctgcaggatg tacacctaac agacctccta gaaaccttac 180cagaaaatgg ggactgggta gggaaggaaa cttaaaagat caacaaactg ccagcccacg 240gactgcagag gctgtcacag ccagatgggg tggccagggt gccacaaacc caaagcaaag 300tttcaaaata atataaaatt taaaaagttt tgtacataag ctattcaaga tttctccagc 360actgactgat acaaagcaca attgagatgg cacttctaga gacagcagct tcaaacccag 420aaaagggtga tgagatgagt ttcacatggc taaatcagtg gcaaaaacac agtcttcttt 480ctttctttct ttcaaggagg caggaaagca attaagtggt cacctcaaca taagggggac 540atgatccatt ctgtaagcag ttgtgaaggg g 571 107 555 DNA Homo sapiens 107caggaaccgg agcgcgagca gtagctgggt gggcaccatg gctgggatca ccaccatcga 60ggcggtgaag cgcaagatcc aggttctgca gcagcaggca gatgatgcag aggagcgagc 120tgagcgcctc cagcgagaag ttgagggaga aaggcgggcc cgggaacagg ctgaggctga 180ggtggcctcc ttgaaccgta ggatccagct ggttgaagaa gagctggacc gtgctcagga 240gcgcctggcc actgccctgc aaaagctgga agaagctgaa aaagctgctg atgagagtga 300gagaggtatg aaggttattg aaaaccgggc cttaaaagat gaagaaaaga tggaactcca 360ggaaatccaa ctcaaagaag ctaagcacat tgcagaagag gcagatagga agtatgaaga 420ggtggctcgt aagttggtga tcattgaagg agacttggaa cgcacagagg aacgagctga 480gctggcagag tcccgttgcc gagagatgga tgagcagatt agactgatgg accagaacct 540gaagtgtctg agtgc 555 108 541 DNA Homo sapiens 108atctacgtca tcaatcaggc tggagacacc atgttcaatc gagctaagct gctcaatatt 60ggctttcaag aggccttgaa ggactatgat tacaactgct ttgtgttcag tgatgtggac 120ctcattccga tggacgaccg taatgcctac aggtgttttt cgcagccacg gcacatttct 180gttgcaatgg acaagttcgg gtttagcctg ccatatgttc agtattttgg aggtgtctct 240gctctcagta aacaacagtt tcttgccatc aatggattcc ctaataatta ttggggttgg 300ggaggagaag atgacgacat ttttaacaga ttagttcata aaggcatgtc tatatcacgt 360ccaaatgctg tagtagggag gtgtcgaatg atccggcatt caagagacaa gaaaaatgag 420cccaatcctc agaggtttga ccggatcgca catacaaagg aaacgatgcg cttcgatggt 480ttgaactcac ttacctacaa ggtgttggat gtcagagata cccgttatat acccaaatca 540c 541 109 411 DNA Homo sapiens 109ctagacctct aattaaaagg cacaatcatg ctggagaatg aacagtctga ccccgagggc 60cacagcgaat tttagggaag gaggcaaaga ggtgagaagg gaaaggaaag aaggaaggaa 120ggagaacaat aagaactgga gacgttgggt gggtcaggga gtgtggtgga ggctcggaga 180gatggtaaac aaacctgact gctatgagtt ttcaacccca tagtctaggg ccatgagggc 240gtcagttctt ggtggctgag ggtccttcca cccagcccac ctgggggagt ggagtgggga 300gttctgccag gtaagcagat gttgtctccc aagttcctga cccagatgtc tggcaggata 360acgctgacct gttccctcaa caagggacct gaaagtaatt ttgctcttta c 411 110 451 DNA Homo sapiens 110ccgaattcaa gcgtcaacga tccytccctt accatcaaat caattggcca ccaatggtac 60tgaacctacg agtacaccga ctacgggcgg actaatcttc aactcctaca tacttccccc 120attattccta gaaccaggcg acctgcgact ccttgacgtt gacaatcgag tagtactccc 180gattgaagcc cccattcgta taataattac atcacaagac gtcttgcact catgagctgt 240ccccacatta ggcttaaaaa cagatgcaat tcccggacgt ctaagccaaa ccactttcac 300cgctacacga ccgggggtat actacggtca atgctctgaa atctgtggag caaaccacag 360tttcatgccc atcgtcctag aattaattcc cctaaaaatc tttgaaatag ggcccgtatt 420taccctatag caccccctct accccctcta g 451 111 541 DNA Homo sapiens 111gctcttcaca cttttattgt taattctctt cacatggcag atacagagct gtcgtcttga 60agaccaccac tgaccaggaa atgccacttt tacaaaatca tccccccttt tcatgattgg 120aacagttttc ctgaccgtct gggagcgttg aagggtgacc agcacatttg cacatgcaaa 180aaaggagtga ccccaaggcc tcaaccacac ttcccagagc tcaccatggg ctgcaggtga 240cttgccaggt ttggggttcg tgagctttcc ttgctgctgc ggtggggagg ccctcaagaa 300ctgagaggcc ggggtatgct tcatgagtgt taacatttac gggacaaaag cgcatcatta 360ggataaggaa cagccacagc acttcatgct tgtgagggtt agctgtagga gcgggtgaaa 420ggattccagt ttatgaaaat ttaaagcaaa caacggtttt tagctgggtg ggaaacagga 480aaactgtgat gtcggccaat gaccaccatt tttctgccca tgtgaaggtc cccatgaaac 540c 541 112 521 DNA Homo sapiens 112caagcgcttg gcgtttggac ccagttcagt gaggttcttg ggttttgtgc ctttggggat 60tttggtttga cccaggggtc agccttagga aggtcttcag gaggaggccg agttcccctt 120cagtaccacc cctctctccc cactttccct ctcccggcaa catctctggg aatcaacagc 180atattgacac gttggagccg agcctgaaca tgcccctcgg ccccagcaca tggaaaaccc 240ccttccttgc ctaaggtgtc tgagtttctg gctcttgagg catttccaga cttgaaattc 300tcatcagtcc attgctcttg agtctttgca gagaacctca gatcaggtgc acctgggaga 360aagactttgt ccccacttac agatctatct cctcccttgg gaagggcagg gaatggggac 420ggtgtatgga ggggaaggga tctcctgcgc ccttcattgc cacacttggt gggaccatga 480acatctttag tgtctgagct tctcaaatta ctgcaatagg a 521 113 568 DNA Homo sapiens 113agcgtcaaat cagaatggaa aagactcaaa accatcatca acaccaagat caaaaggaca 60agratccttc aagaaacagg aaaaaactcc taaaacacca aaaggaccta gttctgtaga 120agacattaaa gcaaaaatgc aagcaagtat agaaaaaggt ggttctcttc ccaaagtgga 180agccaaattc atcaattatg tgaagaattg cttccggatg actgaccaag aggctattca 240agatctctgg cagtggagga agtctcttta agaaaatagt ttaaacaatt tgttaaaaaa 300ttttccgtct tatttcattt ctgtaacagt tgatatctgg ctgtcctttt tataatgcag 360agtgagaact ttccctaccg tgtttgataa atgttgtcca ggttctattg ccaagaatgt 420gttgtccaaa atgcctgttt agtttttaaa gatggaactc caccctttgc ttggttttaa 480gtatgtatgg aatgttatga taggacatag tagtagcggt ggtcagacat ggaaatggtg 540ggsmgacaaa aatatacatg tgaaataa 568 114 483 DNA Homo sapiens 114tccgaattcc aagcgaatta tggacaaacg attcctttta gaggattact tttttcaatt 60tcggttttag taatctaggc tttgcctgta aagaatacaa cgatggattt taaatactgt 120ttgtggaatg tgtttaaagg attgattcta gaacctttgt atatttgata gtatttctaa 180ctttcatttc tttactgttt gcagttaatg ttcatgttct gctatgcaat cgtttatatg 240cacgtttctt taattttttt agattttcct ggatgtatag tttaaacaac aaaaagtcta 300tttaaaactg tagcagtagt ttacagttct agcaaagagg aaagttgtgg ggttaaactt 360tgtattttct ttcttataga ggcttctaaa aaggtatttt tatatgttct ttttaacaaa 420tattgtgtac aacctttaaa acatcaatgt ttggatcaaa acaagaccca gcttattttc 480tgc 483 115 521 DNA Homo sapiens 115tgtggtggcg cgggctgagg tggaggccca ggactctgac cctgcccctg ccttcagcaa 60ggcccccggc agcgccggcc actacgaact gccgtgggtt gaaaaatata ggccagtaaa 120gctgaatgaa attgtcggga atgaagacac cgtgagcagg ctagaggtct ttgcaaggga 180aggaaatgtg cccaacatca tcattgcggg ccctccagga accggcaaga ccacaagcat 240tctgtgcttg gcccgggccc tgctgggccc agcactcaaa gatgccatgt tggaactcaa 300tgcttcaaat gacaggggca ttgacgttgt gaggaataaa attaaaatgt ttgctcaaca 360aaaagtcact cttcccaaag gccgacataa gatcatcatt ctggatgaag cagacagcat 420gaccgacgga gcccagcaag ccttgaggag aaccatggaa atctactcta aaaccactcg 480ttcgcccttg cttgtaatgc ttcggataag atcatcgagc c 521 116 501 DNA Homo sapiens 116ctttgcaaag cttttatttc atgtctgcgg catggaatcc acctgcacat ggcatcttag 60ctgtgaagga gaaagcagtg cacgagaagg aatgagtggg cggaaccaac ggcctccaca 120agctgccttc cagcagcctg ccaaggccat ggcagagaga gactgcaaac aaacacaagc 180aaacagagtc tcttcacagc tggagtctga aagctcatag tggcatgtgt gaatctgaca 240aaattaaaag tgtgcatagt ccattacatg cataaaacac taataataat cctgtttaca 300cgtgactgca gcaggcaggt ccagctccac cactgccctc ctgccacatc acatcaagtg 360ccatggttta gagggttttt catatgtaat tcttttattc tgtaaaaggt aacaaaatat 420acagaacaaa actttccctt tttaaaacta atgttacaaa tctgtattat cacttggata 480taaatagtat ataagctgat c 501 117 451 DNA Homo sapiens modified_base (320) Where n is a, c, g or t 117caagggatat atgttgaggg tacrgrgtga cactgaacag atcacaaagc acgagaaaca 60ttagttctct ccctccccag cgtctccttc gtctccctgg ttttccgatg tccacagagt 120gagattgtcc ctaagtaact gcatgatcag agtgctgkct ttataagact cttcattcag 180cgtatccaat tcagcaattg cttcatcaaa tgccgttttt gccaggctac aggccttttc 240aggagagttt agaatctcat agtaaaagac tgagaaattt agtgccagac caagacgaat 300tgggtgtgta ggctgcattn ctttcttact aatttcaaat gcttcctggt aagcctgctg 360ggagttcgac acaagtggtt tgtttgttgc tccagatgcc acttcagaaa gatacctaaa 420ataatctcct ttcattttca aagtagaaca c 451 118 501 DNA Homo sapiens 118tccggagccg gggtagtcgc cgccgccgcc gccggtgcag ccactgcagg caccgctgcc 60gccgcctgag tagtgggctt aggaaggaag aggtcatctc gctcggagct tcgctcggaa 120gggtctttgt tccctgcagc cctcccacgg gaatgacaat ggataaaagt gagctggtac 180agaaagccaa actcgctgag caggctgagc gatatgatga tatggctgca gccatgaagg 240cagtcacaga acaggggcat gaactctcca acgaagagag aaatctgctc tctgttgcct 300acaagaatgt ggtaaggccg cccgccgctc ttcctggcgt gtcatctcca gcattgagca 360gaaaacagag aggaatgaga agaagcagca gatgggcaaa gagtaccgtg agaagataga 420ggcagaactg caggacatct gcaatgatgt tctggagctt gttggacaaa tatcttattc 480caatgctaca caacccagaa a 501 119 391 DNA Homo sapiens 119aaaaagcagc argttcaaca caaaatagaa atctcaaatg taggatagaa caaaaccaag 60tgtgtgaggg gggaagcaac agcaaaagga agaaatgaga tgttgcaaaa aagatggagg 120agggttcccc tctcctctgg ggactgactc aaacactgat gtggcagtat acaccattcc 180agagtcaggg gtgttcattc ttttttggga gtaagaaaag gtggggatta agaagacgtt 240tctggaggct tagggaccaa ggctggtctc tttcccccct cccaaccccc ttgatccctt 300tctctgatca ggggaaagga gctcgaatga gggaggtaga gttggaaagg gaaaggattc 360cacttgacag aatgggacag actccttccc a 391 120 421 DNA Homo sapiens modified_base (409) Where n is a, c, g or t 120tggcaatagc acagccatcc aggagctctt cargcgcatc tcggagcagt tcactgccat 60gttccgccgg aaggccttcc tccactggta cacaggcgag ggcatggacg agatggagtt 120caccgaggct gagagcaaca tgaacgacct cgtctctgag tatcaagcag taccaggatg 180ccaccgcaga agaggaggag gatttcggtg aggaggccga agaggaggcc taaggcagag 240cccccatcac ctcaggcttc tcagttccct tagccgtctt actcaactgc ccctttcctc 300tccctcagaa tttgtgtttg ctgcctctat cttgtttttt gttttttctt ctgggggggt 360ctagaacagt gcctggcaca tagtaggcgc tcaataaata cttggttgnt gaatgtctcc 420t 421 121 206 DNA Homo sapiens 121agctggcgct agggctcggt tgtgaaatac agcgtrgtca gcccttgcgc tcagtgtaga 60aacccacgcc tgtaaggtcg gtcttcgtcc atctgctttt ttctgaaata cactaagagc 120agccacaaaa ctgtaacctc aaggaaacca taaagcttgg agtgccttaa tttttaacca 180gtttccaata aaacggttta ctacct 206 122 131 DNA Homo sapiens 122ggagatgaag atgaggaagc tgagtcagct acgggcargc gggcagctga agatgatgag 60gatgacgatg tcgataccaa gaagcagaag accgacgagg atgactagac agcaaaaaag 120gaaaagttaa a 131 123 231 DNA Homo sapiens modified_base (166) Where n is a, c, g or t 123gatgaaaatt aaatacttaa attaatcaaa aggcactacg ataccaccta aaacctactg 60cctcagtggc agtakgctaa kgaagatcaa gctacagsac atyatctaat atgaatgtta 120gcaattacat akcargaagc atgtttgctt tccagaagac tatggnacaa tggtcattwg 180ggcccaagag gatatttggc cnggaaagga tcaagataga tnaangtaaa g 231 124 521 DNA Homo sapiens modified_base (284) Where n is a, c, g or t 124gagtagcaac gcaaagcgct tggtattgag tctgtgggsg acttcggttc cggtctctgc 60agcagccgtg atcgcttagt ggagtgctta gggtagttgg ccaggatgcc gaatatcaaa 120atcttcagca ggcagctccc accaggactt atctcasaaa attgctgacc gcctgggcct 180ggagctaggc aaggtggtga ctaagaaatt cagcaaccag gagacctgtg tggaaattgg 240tgaaagtgta ccgtggagag gatgtctaca ttgttcagag tggntgtggc gaaatcaatg 300acaatttaat ggagcttttg atcatgatta atgcctgcaa gattgcttca gccagccggg 360ttactgcagt catcccatgc ttcccttatg ccccggcagg ataagaaaga tnagagccgg 420gccgccaatc tcagccaagc ttggtgcaaa tatgctatct gtagcagtgc agatcatatt 480atcaccatgg acctacatgc ttctcaaatt canggctttt t 521 125 341 DNA Homo sapiens modified_base (277) Where n is a, c, g or t 125atgcaaaagg ggacacaggg ggttcaaaaa taaaaatttc tcttccccct ccccaaacct 60gtaccccagc tccccgacca caaccccctt cctcccccgg ggaaagcaag aaggagcagg 120tgtggcatct gcagctggga agagagaggc cggggaggtg ccgagctcgg tgctggtctc 180tttccaaata taaatacgtg tgtcagaact ggaaaatcct ccagcaccca ccacccaagc 240actctccgtt ttctgccggt gtttggagag gggcggnggg caggggcgcc aggcaccggc 300tggctgcggt ctactgcatc cgctgggtgt gcaccccgcg a 341 126 521 DNA Homo sapiens modified_base (353) Where n is a, c, g or t 126aggttggaga aggtcatgca ggtgcagatt gtccaggskc agccacaggg tcaagcccaa 60caggcccaga gtggcactgg acagaccatg caggtgatgc agcagatcat cactaacaca 120ggagagatcc agcagatccc ggtgcagctg aatgccggcc agctgcagta tatccgctta 180gcccagcctg tatcaggcac tcaagttgtg cagggacaga tccagacact tgccaccaat 240gctcaacaga ttacacagac agaggtccag caaggacagc agcagttcaa gccagttcac 300aagatggaca gcagctctac cagatccagc aagtcaccat gcctgcgggc cangacctcg 360ccagcccatg ttcatccagt caagccaacc agcccttcna cgggcaggcc ccccaggtga 420ccggcgactg aagggcctga gctggcaagg ccaangacac ccaacacaat ttttgccata 480cagcccccag gcaatgggca cagcctttct tcccagagga c 521 127 351 DNA Homo sapiens 127tgagatttat tgcatttcat gcagcttgaa gtccatgcaa aggrgactag cacagttttt 60aatgcattta aaaaataaaa gggaggtggg cagcaaacac acaaagtcct agtttcctgg 120gtccctggga gaaaagagtg tggcaatgaa tccacccact ctccacaggg aataaatctg 180tctcttaaat gcaaagaatg tttccatggc ctctggatgc aaatacacag agctctgggg 240tcagagcaag ggatggggag aggaccacga gtgaaaaagc agctacacac attcacctaa 300ttccatctga gggcaagaac aacgtggcaa gtcttggggg tagcagctgt t 351 128 521 DNA Homo sapiens 128tccagacatg ctcctgtcct aggcggggag caggaaccag acctgctatg ggaagcagaa 60agagttaagg gaaggtttcc tttcattcct gttccttctc ttttgctttt gaacagtttt 120taaatatact aatagctaag tcatttgcca gccaggtccc ggtgaacagt agagaacaag 180gagcttgcta agaattaatt ttgctgtttt tcaccccatt caaacagagc tgccctgttc 240cctgatggag ttccattcct gccagggcac ggctgagtaa cacgaagcca ttcaagaaag 300gcgggtgtga aatcactgcc accccatgga cagacccctc actcttcctt cttagccgca 360gcgctactta ataaatatat ttatactttg aaattatgat aaccgatttt tcccatgcgg 420catcctaagg gcacttgcca gctcttatcc ggacagtcaa gcactgttgt tggacaacag 480ataaaggaaa agaaaaagaa gaaaacaacc gcaacttctg t 521 129 521 DNA Homo sapiens 129tgagacggac cactggcctg gtcccccctc atktgctgtc gtaggacctg acatgaaacg 60cagatctagt ggcagagagg aagatgatga ggaacttctg agacgtcggc agcttcaaga 120agagcaatta atgaagctta actcaggcct gggacagttg atcttgaaag aagagatgga 180gaaagagagc cgggaaaggt catctctgtt agccagtcgc tacgattctc ccatcaactc 240agcttcacat attccatcat ctaaaactgc atctctccct ggctatggaa gaaatgggct 300tcaccggcct gtttctaccg acttcgctca gtataacagc tatggggatg tcagcggggg 360agtgcgagat taccagacac ttccagatgg ccacatgcct gcaatgagaa tggaccgagg 420agtgtctatg cccaacatgt tggaaccaaa gatatttcca tatgaaatgc tcatggtgac 480caacagaggg ccgaaaccaa atctcagaga ggtggacaga a 521 130 270 DNA Homo sapiens 130tcactttatt tttcttgtat aaaaacccta tgttgtagcc acagctggag cctgagtccg 60ctgcacggag actctggtgt gggtcttgac gaggtggtca gtgaactcct gatagggaga 120cttggtgaat acagtctcct tccagaggtc gggggtcagg tagctgtagg tcttagaaat 180ggcatcaaag gtggccttgg cgaagttgcc cagggtggca gtgcagcccc gggctgaggt 240gtagcagtca tcgataccag ccatcatgag 270 131 341 DNA Homo sapiens 131ctggaatata gacccgtgat cgacaaaact ttgaacgagg ctgactgtgc caccgtcccg 60ccagccattc gctcctactg atgagacaag atgtggtgat gacagaatca gcttttgtaa 120ttatgtataa tagctcatgc atgtgtccat gtcataactg tcttcatacg cttctgcact 180ctggggaaga aggagtacat tgaagggaga ttggcaccta gtggctggga gcttgccagg 240aacccagtgg ccagggagcg tggcacttac ctttgtccct tgcttcattc ttgtgagatg 300ataaaactgg gcacagctct taaataaaat ataaatgaac a 341 132 844 DNA Homo sapiens modified_base (37) Where n is a, c, g or t 132tgaatgggga ggagctgacc caggaaatgg agcttgngga gaccaggcct gcaggggatg 60gaaccttcca gaagtgggca tctgtggtgg tgcctcttgg gaaggagcag aagtacacat 120gccatgtgga acatgagggg ctgcctgagc ccctcaccct gagatggggc aaggaggagc 180ctccttcatc caccaagact aacacagtaa tcattgctgt tccggttgtc cttggagctg 240tggtcatcct tggagctgtg atggcttttg tgatgaagag gaggagaaac acaggtggaa 300aaggagggga ctatgctctg gctccaggct cccagagctc tgatatgtct ctcccagatt 360gtaaagtgtg aagacagctg cctggtgtgg acttggtgac agacaatgtc ttcacacatc 420tcctgtgaca tccagagacc tcagttctct ttagtcaagt gtctgatgtt ccctgtgagt 480ctgcgggctc aaagtgaaga actgtggagc ccagtccacc cctgcacacc aggaccctat 540ccctgcactg ccctgtgttc ccttccacag ccaaccttgc tgctccagcc aaacattggt 600ggacatctgc agcctgtcag ctccatgcta ccctgacctt caactcctca cttccacact 660gagaataata atttgaatgt gggtggctgg agagatggct cagcgctgac tgctcttcca 720aaggtcctga gttcaaatcc cagcaaccac atggtggctc acaaccatct gtaatgggat 780ctaataccct cttctgcagt gtctgaagac asctacagtg tacttacata taataataaa 840taag 844 133 601 DNA Homo sapiens 133ggccgggcgc gcgcgccccc gccacacgca cgccgggcgt gccagtttat aaagggagag 60agcaagcagc gagtcttgaa gctctgtttg gtgctttgga tccatttcca tcggtcctta 120cagccgctcg tcagactcca gcagccaaga tggtgaagca gatcgagagc aagactgctt 180ttcaggaagc cttggacgct gcaggtgata aacttgtagt agttgacttc tcagccacgt 240ggtgtgggcc ttgcaaaatg atcaagcctt tctttcattc cctctctgaa aagtattcca 300acgtgatatt ccttgaagta gatgtggatg actgtcagga tgttgcttca gagtgtgaag 360tcaaatgcat gccaacattc cagtttttta agaagggaca aaaggtgggt gaattttctg 420gagccaataa ggaaaagctt gaagccacca ttaatgaatt agtctaatca tgttttctga 480aaatataacc agccattggc tatttaaaac ttgtaatttt tttaatttac aaaaatataa 540aatatgaaga cataaacccm gttgccatct gcgtgacaat aaaacattaa tgctaacact 600t 601 134 421 DNA Homo sapiens 134tcacataaga aatttaagca agttacrcta tcttaaaaaa cacaacgaat gcattttaat 60agagaaaccc ttccctccct ccacctccct cccccaccct cctcatgaat taagaatcta 120agagaagaag taaccataaa accaagtttt gtggaatcca tcatccagag tgcttacatg 180gtgattaggt taatattgcc ttcttacaaa atttctattt taaaaaaaat tataaccttg 240attgcttatt acaaaaaaat tcagtacaaa agttcaatat attgaaaaat gcttttcccc 300tccctcacag caccgtttta tatatagcag agaataatga agagattgct agtctagatg 360gggcaatctt caaattacac caagacgcac agtggtttat ttaccctccc cttctcataa 420g 421 135 511 DNA Homo sapiens 135ggaaaggatt caagaattag aggacttgct tgctrragaa aaagacaact ctcgtcgcat 60gctgacagac aaagagagag agatggcgga aataagggat caaatgcagc aacagctgaa 120tgactatgaa cagcttcttg atgtaaagtt agccctggac atggaaatca gtgcttacag 180gaaactctta gaaggcgaag aagagaggtt gaagctgtct ccaagccctt cttcccgtgt 240gacagtatcc cgagcatcct caagtcgtag tgtaccgtac aactagagga aagcggaaga 300gggttgatgt ggaagaatca gaggcgaagt agtagtgtta gcatctctca ttccgcctca 360accactggaa atgtttgcat cgaagaaatt gatgttgatg ggaaatttat cccgcttgaa 420gaacacttct gaacaggatc aaccaatggg aaggcttggg agatgatcag aaaaattgga 480gacacatcag tcagttataa atatacctca a 511 136 341 DNA Homo sapiens 136catgggtttc accaggttgg ccaggctgct cttgaactsc tgacctcagg tgatccaccc 60gcctcggcct cccaaagtgc tgggattaca ggcgtgagcc accacgcccg gcccccaaag 120ctgtttcttt tgtctttagc gtaaagctct cctgccatgc agtatctaca taactgacgt 180gactgccagc aagctcagtc actccgtggt ctttttctct ttccagttct tctctctctc 240ttcaagttct gcctcagtga aagctgcagg tccccagtta agtgatcagg tgagggttct 300ttgaacctgg ttctatcagt cgaattaatc cttcatgatg g 341 137 551 DNA Homo sapiens 137gatgtgttgg accctctgtg tcaaaaaaaa cctcacaaag aatcccctgc tcattacaga 60agaagatgca tttaaaatat gggttatttt caacttttta tctgaggaca agtatccatt 120aattattgtg tcagaagaga ttgaatacct gcttaagaag cttacagaag ctatgggagg 180aggttggcag caagaacaat ttgaacatta taaaatcaac tttgatgaca gtaaaaatgg 240cctttctgca tgggaactta ttgagcttat tggaaatgga cagtttagca aaggcatgga 300ccggcagact gtgtctatgg caattaatga agtctttaat gaacttatat tagatgtgtt 360aaagcagggt tacatgatga aaaagggcca cagacggaaa aactggactg aaagatggtt 420tgtactaaaa cccaacataa tttcttacta tgtgagtgag gatctgaagg ataagaaagg 480agacattctc ttggatgaaa attgctgtgt agaagtcctt gcctgacaaa agatggaaag 540aaatgccttt t 551 138 531 DNA Homo sapiens modified_base (490) Where n is a, c, g or t 138gactggttct ttatttcaaa aagacacttg tcaatattca gtrtcaaaac agttgcacta 60ttgatttctc tttctcccaa tcggccccaa agagaccaca taaaaggaga gtacatttta 120agccaataag ctgcaggatg tacacctaac agacctccta gaaaccttac cagaaaatgg 180ggactgggta gggaaggaaa cttaaaagat caacaaactg ccagcccacg gactgcagag 240gctgtcacag ccagatgggg tggccagggt gccacaaacc caaagcaaag tttcaaaata 300atataaaatt taaaaagttt tgtacataag ctattcaaga tttctccagc actgactgat 360acaaagcaca attgagatgg cacttctaga gacagcagct tcaaacccag aaaagggtga 420tgagatgaag tttcacatgg ctaaatcagt ggcaaaaaca cagtcttctt tctttctttc 480tttcaaggan gcaggaaagc aattaagtgg tcaccttaac ataaggggga c 531 139 521 DNA Homo sapiens modified_base (517) Where n is a, c, g or t 139tgggtgggca ccatggctgg gatcaccacc atcgaggcgg tgaagcgcaa gatccaggtt 60ctgcagcagc aggcagatga tgcagaggag cgagctgagc gcctccagcg agaagttgag 120ggagaaaggc gggcccggga acaggctgag gctgaggtgg cctccttgaa ccgtaggatc 180cagctggttg aagaagagct ggaccgtgct caggagcgcc tggccactgc cctgcaaaag 240ctggaagaag ctgaaaaagc tgctgatgag agtgagagag gtatgaaggt tattgaaaac 300cgggccttaa aagatgaaga aaagatggaa ctccaggaaa tccaactcaa agaagctaag 360cacattgcag aagaggcaga taggaagtat gaagaggtgg ctcgtaagtt ggtgatcatt 420gaaggagact tggaaccgca cagaaggaac gagcttgagc ttggcaaaag tcccgttgcc 480cagagatggg atgaaccaga ttagactgat ggaccanaac c 521 140 571 DNA Homo sapiens modified_base (7) Where n is a, c, g or t 140aggggcngcg ggtgcgtggg ccactgggtg accgacttag cctggccaga ctctcagcac 60ctggaagcgc cccgagagtg acagcgtgag gctgggaggg aggacttggc ttgagcttgt 120taaactctgc tctgagcctc cttgtcgcct gcatttagat ggctcccgca aagaagggtg 180gcgagaagaa aaagggccgt tctgccatca acgaagtggt aacccgagaa tacaccatca 240acattcacaa gcgcatccat ggagtgggct tcaagaagcg tgcacctcgg gcactcaaag 300agattcggaa atttgccatg aaggagatgg gaactccaga tgtgcgcatt gacaccaggc 360tcaacaaagc tgtctgggcc aaaggaataa ggaatgtgcc ataccgaatc cggtgtgcgg 420ctgtccagaa aacgtaatga ggatgaagat tcaccaaata agctatatac tttggttacc 480tatgtacctg ttaccacttt caaaaatcta cagacagtca atgtggatga gaactaatcg 540ctgatcgtca gatcaaataa agttataaaa t 571 141 531 DNA Homo sapiens 141tcgggagcca cacttggccc tcttcctctc caaagsgcca gaacctcctt ctctttggag 60aatggggagg cctcttggag acacagaggg tttcaccttg gatgacctct agagaaattg 120cccaagaagc ccaccttctg gtcccaacct gcagacccca cagcagtcag ttggtcaggc 180cctgctgtag aaggtcactt ggctccattg cctgcttcca accaatgggc aggagagaag 240gcctttattt ctcgcccacc cattcctcct gtaccagcac ctccgttttc agtcagtgtt 300gtccagcaac ggtaccgttt acacagtcac ctcagacaca ccatttcacc tcccttgcca 360agctgttagc cttagagtga ttgcagtgaa cactgtttac acaccgtgaa tccattccca 420tcagtccatt ccagttggca ccagcctgaa ccatttggta cctggtgtta actggagtcc 480tgtttacaag gtggagtcgg ggcttgctga cttctcttca tttgagggca c 531 142 491 DNA Homo sapiens modified_base (410) Where n is a, c, g or t 142acctagacag aaggtgggtg agggaggact ggtaggaggc tgaggcaatt ccttggtagt 60ttgtcctgaa accctactgg agaagtcagc atgaggcacc tactgagaga agtgcccaga 120aactgctgac tgcatctgtt aagagttaac agtaaagagg tagaagtgtg tttctgaatc 180agagtggaag cgtctcaagg gtcccacagt ggaggtccct gagctacctc ccttccgtga 240gtgggaagag tgaagcccat gaagaactga gatgaagcaa ggatggggtt cctgggctcc 300aggcaagggc tgtgctctct gcagcaggga gccccacgag tcagaagaaa agaactaatc 360atttgttgca agaaaccttg cccggatact agcggaaaac tggaggcggn ggtgggggca 420caggaaagtg gaagtgattt gatggagagc agagaagcct atgcacagtg gccgagtcca 480cttgtaaagt g 491 143 515 DNA Homo sapiens 143ttcaagcaat tgtaacaagt atatgtagat tagagtgagc aaaatcatat acaattttca 60tttccagttg ctattttcca aattgttctg taatgtcgtt aaaattactt aaaaattaac 120aaagccaaaa attatattta tgacaagaaa gccatcccta cattaatctt acttttccac 180tcaccggccc atctccttcc tctttttcct aactatgcca ttaaaactgt tctactgggc 240cgggcgtgtg gctcatgcct gtaatcccag cattttggga ggccaaggca ggcggatcat 300gaggtcaaga gattgagacc atcctggcca acatggtgaa accccgcctc gactaagaat 360acaaaaatta gctgggcatg gtggcgcatg cctgtagtct cagctactcg ggaggctgag 420gcagaagaat cgcttgaacc cgggaggcag aggatgcagt gagccccgat cgcgccactg 480cactctagcc tgggcgacag actgagactc tgctc 515 144 340 DNA Homo sapiens 144tgtgccagtc tacaggccta tcagcagcga ctccttcagc aacagatggg gtcccctgtt 60cagcccaacc ccatgagccc ccagcagcat atgctcccaa atcaggccca gtccccacac 120ctacaaggcc agcagatccc taattctctc tccaatcaag tgcgctctcc ccagcctgtc 180ccttctccac ggccacagtc ccagcccccc cactccagtc cttccccaag gatgcagcct 240cagccttctc cacaccacgt ttccccacag acaagttccc cacatcctgg actggtagtt 300gcccaggcca accccatgga acaagggcat tttgccagcc 340 145 630 DNA Homo sapiens 145tgtaaaaact tgtttttaat tttgtataaa ataaaggtgg tccatgccca cgggggctgt 60aggaaatcca agcagaccag ctggggtggg gggatgtagc ctacctcggg ggactgtctg 120tcctcaaaac gggctgagaa ggcccgtcag gggcccaggt cccacagaga ggcctgggat 180actcccccaa cccgaggggc agactgggca gtggggagcc cccatcgtgc cccagaggtg 240gccacaggct gaaggagggg cctgaggcac cgcagcctgc aacccccagg gctgcagtcc 300actaactttt tacagaataa aaggaacatg gggatgggga aaaaagcacc aggtcaggca 360gggcccgagg gccccagatc ccaggagggc caggactcag gatgccagca ccaccctagc 420agctcccaca gctcctggca caggaggccg ccacggattg gcacaggccg ctgctggcca 480tcacgccaca tttggagaac ttgtcccgac agaggtcagc tcggaggagc tcctcgtggg 540cacacactgt acgaacacag atctccttgt taatgacgta cacacggcgg aggctgcggg 600gacagggcac gggaggtctc agccccactt 630 146 521 DNA Homo sapiens 146atggctgctg gatttaggtg gtaatagggg ctgtgggcca taaatctgaa gccttgagaa 60ccttgggtct ggagagccat gaagagggaa ggaaaagagg gcaagtcctg aacctaacca 120atgacctgat ggattgctcg accaagacac agaagtgaag tctgtgtctg tgcacttccc 180acagactgga gtttttggtg ctgaatagag ccagttgcta aaaaattggg ggtttggtga 240agaaatctga ttgttgtgtg tattcaatgt gtgattttaa aaataaacag caacaacaat 300aaaaaccctg actggctgtt ttttccctgt attctttaca actatttttt gaccctctga 360aaattattat acttcaccta aatggaagac tgctgtgttt gtggaaattt tgtaattttt 420taatttattt tattctctct cctttttatt ttgcctgcag aatccgttga gagactaata 480aggcttaata tttaattgat ttgtttaata tgtatataaa t 521 147 562 DNA Homo sapiens 147ggcatgcgag cgcactcggc ggacgcaagg gcggcgggga gcacacggag cactgcaggc 60gccgggttgg gacagcgtct tcgctgctgc tggatagtcg tgttttcggg gatcgaggat 120actcaccaga aaccgaaaat gccgaaacca atcaatgtcc gagttaccac catggatgca 180gagctggagt ttgcaatcca gccaaataca actggaaaac agctttttga tcaggtggta 240aagactatcg gcctccggga agtgtggtac tttggcctcc actatgtgga taataaagga 300tttcctacct ggctgaagct ggataagaag gtgtctgccc aggaggtcag gaaggagaat 360cccctccagt tcaagttccg ggccaaagtt ctaccctgaa gatgtggctg aggagctcat 420ccaggacatc acccagaaac ttttcttcct tcaagtgaag gaaggaatcc ttagcgatga 480gatctactgc cccccttgar actgccgtgc tcttggggtc ctacgcttgt gcatgccaag 540tttggggact accaccaaga ag 562 148 820 DNA Homo sapiens 148gaaggagtcg ggatactcag cattgatgca ccccaatttc aaagcggcat tcttcggcag 60gtctctggga caatctctag ggtcactacc tggaaactcg ttagggtaca actgaatgct 120gaaaggaaag aacacctgca gaaccggaca gaaattcacc ccggcgatca gctgattgat 180ctcggtcgac cagaagtcat ggctaaagat gacgaggacg ttgtcaattc cctgggcttt 240tcgaagtgag tccagcagca gtctgaggta ttcgggccgg ttatgcacct ggaccaccag 300caccagctcc cggggggccc aggtgccagc cttatctaca ttcctcaggg tctgatcaaa 360gttcagctgg tacaccaggg accggtaccg cagcgtcagg ttgtccgctc gggctggggg 420accgccggga ccagggaagc cgccgacacg ttggagaccc tgcggatgcc cacagccaca 480gaggggtggt ccccaccgcg gccgccggca ccccgcgcgg gttcggcgtc cagcaacggt 540ggggcgaggg cctcgttctt cctttgtcgc ccattgctgc tccagaggac gaagccgcag 600gcggccacca cgagcgtcag gattagcacc ttccgtttgt agatgcggaa cctcatggtc 660tccagggccg ggagcgcagc tacagctcga gcgtcggcgc cgccgctagg agccgcggct 720cggcttcgtc tccgtcctct ccattcagca ccacgggtcc cggaaaaagc tcagccscgg 780tcccaaccgc accctagctt cgttacctgc gcctcgcttg 820 149 501 DNA Homo sapiens 149cagattttta tttgcagtcg tcactggggc cgtttcttgc tgcttatttg tctgctagcc 60tgctcttcca gctgcatggc caggcgcaag gccttgatga catctcgcag ggctgagaaa 120tgcttggctt gctgggccag agcagattcc gctttgttca caaaggtctc caggtcatag 180tctggctgct cggtcatctc agagagctca agccagtctg gtccttgctg tatgatctcc 240ttgagctctt ccatagcctt ctcctccagc tccctgatct gagtcatggc ttcgttaaag 300ctggacatct gggaagacag ttcctcctct tccttggata aattgcctgg aatcagcgcc 360ccgttagagc aggcttccat ctcttctgtt tccatttgaa tcaactgctc tccactgggc 420ccactgtggg ggctcagctc cttgaccctg ctgcatatct taagggtgtt taaaggatat 480tcacaggagc ttatgcctgg t 501 150 511 DNA Homo sapiens modified_base (457) Where n is a, c, g or t 150ctcctcttgg tacatgaacc caagttgaaa gtggacttaa caaagtatct ggagaaccaa 60gcattctgct ttgactttgc atttgatgaa acagcttcga atgaagttgt ctacaggttc 120acagcaaggc cactggtaca gacaatcttt gaaggtggaa aagcaacttg ttttgcatat 180ggccagacag gaagtggcaa gacacatact atgggcggag acctctctgg gaaagcccag 240aatgcatcca aagggatcta tgccatggcc ttccgggacg tcttcttctg aagaatcaac 300cctgctaccg gaagttgggc ctggaagtct atgtgacatt cttcgagatc tacaatggga 360agctgtttga cctgctcaac aagaaggcca agcttgcgcg tgctggaaga cggcaagcaa 420caggtgcaag tggtgggggc ttgcaggaac atctggntaa ctctgcttga tgatggcant 480caagatgatc gacatgggca gcgcctgcag a 511 151 566 DNA Homo sapiens 151tcccgaattc aagcgacaaa ttggawagtg aaatggaaga tgcctatcat gaacatcagg 60caaatctttt gcgccaagat ctgatgagac gacaggaaga attaagacgc atggaagaac 120ttcacaatca agaaatgcag aaacgtaaag aaatgcaatt gaggcaagag gaggaacgac 180gtagaagaga ggaagagatg atgattcgtc aacgtgagat ggaagaacaa atgaggcgcc 240aaagagagga aagttacagc cgaatgggct acatggatcc acgggaaaga gacatgcgaa 300tgggtggcgg aggagcaatg aacatgggag atccctatgg ttcaggaggc cagaaatttc 360cacctctagg aggtggtggt ggcataggtt atgaagctaa tcctggcgtt ccaccagcaa 420ccatgagtgg ttccatgatg ggaagtgaca tgcgtactga gcgctttggg cagggaggtg 480cggggcctgt gggtggacag ggtcctagag gaatggggcc tggaactcca gcaggatatg 540gtagagggag agaagagtac gaaggc 566 152 518 DNA Homo sapiens 152ttcgtgaaga ccctgactgg taagaccatc actctcgaag tggagcccga gtgacaccat 60tgagaatgtc aaggcaaaga tccaagacaa ggaaggcatc cctcctgacc agcakaggtt 120gatctttgct gggaaacagc tggaagatgg acgcaccctg tctgactaca acatccagaa 180agagtccacc ctgcacctgg tgctccgtct cagaggtggg atgcaaatct tcgtgaagac 240cctgactggt aagaccatca ccctcgaggt ggagcccagt gacaccatcg agaatgtcaa 300ggcaaagatc caagataagg aaggcatccc tcctgatcag cagaggttga tctttgctgg 360gaaacagctg gaagatggac gcaccctgtc tgactacaac atccagaaag agtccactct 420gcacttggtc ctgcgcttga gggggggtgt ctaagtttcc ccttttaagg tttcaacaaa 480tttcattgca ctttcctttc aataaagttg ttgcattc 518 153 542 DNA Homo sapiens 153gcgcgggtgc gtgggccact gggtgaccga cttagcctgg ccagactctc agcacctgga 60agcgccccga gagtgacagc gtgaggctgg gagggaggac ttggcttgag cttgttaaac 120tctgctctga gcctccttgt cgcctgcatt tagatggctc ccgcaaagaa gggtggcgag 180aagaaaaagg gccgttctgc catcaacgaa gtggtaaccc gagaatacac catcaacatt 240cacaagcgca tccatggagt gggcttcaag aagcgtgcac ctcgggcact caaagagatt 300cggaaatttg ccatgaagga gatgggaact ccagatgtgc gcattgacac caggctcaac 360aaagctgtct gggccaaagg aataaggaat gtgccatacc gaatccgtgt gcggctgtcc 420agaaaacgta atgaggatga agattcacca aataagctat atactttggt tacctatgta 480cctgttacca ctttcaaaaa tctacagaca gtcaatgtgg atgagaacta atcgctgatc 540gt 542 154 411 DNA Homo sapiens 154aattctttat ttaaatcaac aaactcatct tcctcaagcc ccagaccatg gtaggcagcc 60ctccctctcc atcccctcac cccacccctt agccacagtg aagggaatgg aaaatgagaa 120gccacgaggg cccctgccag ggaaggctgc cccagatgtg tggtgagcac agtcagtgca 180gctgtggctg gggcagcagc tgccacaggc tcctccctat aaattaagtt cctgcagcca 240cagctgtggg agaagcatac ttgtagaagc aaggccagtc cagcatcaga aggcagaggc 300agcatcagtg actcccagcc atggaatgaa cggaggacac agagctcaga gacagaacag 360gccaggggga agaaggagag acagaatagg ccagggcatg gcggtgaggg a 411 155 421 DNA Homo sapiens modified_base (173) Where n is a, c, g or t 155tgatgaatct gggtgggctg gcagtagccc gagatgatgg gctcttctct ggggatccca 60actggttccc taagaaatcc aaggagaatc ctcggaactt ctcggataac cagctgcaag 120agggcaagaa cgtgatcggg ttacagatgg gcaccaaccg cggggcgtct cangcaggca 180tgactggcta cgggatgcca cgccagatcc tctgatccca ccccaggcct tgcccctgcc 240ctcccacgaa tggttaatat atatgtagat atatatttta gcagtgacat tcccagagag 300ccccagagct ctcaagctcc tttctgtcag ggtggggggt tcaagcctgt cctgtcacct 360ctgaagtgcc tgctggcatc ctctccccca tgcttactaa tacattccct tccccatagc 420c 421 156 670 DNA Homo sapiens 156agcggagctc cctcccctgg tggctacaac ccacacacgc caggctcagg catcgagcag 60aactccagcg actgggtaac cactgacatt caggtgaagg tgcgggacac ctacctggat 120acacaggtgg tgggacagac aggtgtcatc cgcagtgtca cggggggcat gtgctctgtg 180tacctgaagg acagtgagaa ggttgtcagc atttccagtg agcacctgga gcctatcacc 240cccaccaaga acaacaaggt gaaagtgatc ctgggcgagg atcgggaagc cacgggcgtc 300ctactgagca ttgatggtga ggatggcatt gtccgtatgg accttgatga gcagctcaag 360atcctcaacc tccgcttcct ggggaagctc ctggaagcct gaagcaggca gggccggtgg 420acttcgtcgg atgaagagtg atcctccttc cttccctggc ccttggctgt gacacaagat 480cctcctgcag ggctaggcgg attgttctgg atttcctttt gtttttcctt ttaggtttcc 540atcttttccc tccctggtgc tcattggaat ctgagtagag tctgggggag ggtccccacc 600ttcctgtacc tcctccccac agcttgcttt tgttgtaccg tctttcaata aaaagaagct 660gtttggtcta 670 157 421 DNA Homo sapiens 157ggttcacagc actgctgctt gtgtgttgcc ggccaggaat tccaggctca caaggctatc 60ttagcagctc gttctccggt ttttagtgcc atgtttgaac atgaaatgga ggagagcaaa 120aagaatcgag ttgaaatcaa tgatgtggag cctgaagttt ttaaggaaat gatgtgcttc 180atttacacgg ggaaggctcc aaacctcgac aaaatggctg atgatttgct ggcagctgct 240gacaagtatg ccctggagcg cttaaaggtc atgtgtgagg atgccctctg cagtaacctg 300tccgtggaga acgctgcaga aattctcatc ctggccgacc tccacagtgc agatcagttg 360aaaactcagg cagtggattt catcaactat catgcttcgg atgtcttgga gacctcttgg 420g 421 158 321 DNA Homo sapiens 158tcgtagccat ttttctgctt ctttggagaa tgacgccaca ctgactgctc attgtcgttg 60gttccatgcc aattggtgaa atagaacctc atccggtagt ggagccggag ggacatcttg 120tcatcaacgg tgatggtgcg atttggagca taccagagct tggtgttctc gccatacagg 180gcaaagaggt tgtgacaaag aggagagata cggcatgcct gtgcagccct gatgcacagt 240tcctctgctg tgtactctcc actgcccagc cggaggggct ccctgtccga cagatagaag 300atcacttcca cccctggctt g 321 159 596 DNA Homo sapiens 159tggcacactg ctcttaagaa actatgawga tctgagattt ttttgtgtat gtttttgact 60cttttgagtg gtaatcatat gtgtctttat agatgtacat acctccttgc acaaatggag 120gggaattcat tttcatcact gggagtgtcc ttagtgtata aaaaccatgc tggtatatgg 180cttcaagttg taaaaatgaa agtgacttta aaagaaaata ggggatggtc caggatctcc 240actgataaga ctgtttttaa gtaacttaag gacctttggg tctacaagta tatgtgaaaa 300aaatgagact tactgggtga ggaaattcat tgtttaaaga tggtcgtgtg tgtgtgtgtg 360tgtgtgtgtg ttgtgttgtg ttttgttttt taagggaggg aatttattat ttaccgttgc 420ttgaaattac tgkgtaaata tatgtytgat aatgatttgc tytttgvcma ctaaaattag 480gvctgtataa gtwctaratg cmtccctggg kgttgatytt ccmagatatt gatgatamcc 540cttaaaattg taaccygcct ttttcccttt gctytcmatt aaagtctatt cmaaag 596 160 515 DNA Homo sapiens 160gggggtaggc tctttattag acggttattg ctgtactaca gggtcagagt gcagtgtaag 60cagtgtcaga ggcccgcgtt cagcccaaga atgtggattt tctctcccta ttgatcacag 120tgggtgggtt tcttcagaaa agccccagag gcagggacca gtgagctcca aggttagaag 180tggaactgga aggcttcagt cacatgctgc ttccacgctt ccaggctggg cagcaaggag 240gagatgccca tgacgtgcca ggtctcccca tctgacacca gtgaagtctg gtaggacagc 300agccgcacgc ctgcctctgc caggaggcca atcatggtag gcagcattgc agggtcagag 360gtctgagtcc ggaataggag caggggcagg tccctgcgga gaggcacttc tggcctgaag 420acagctccat tgagcccctg cagtacaggy gtagtgcctt ggaccaagcc cacagcctgg 480taaggggcgc ctgccagggc cacggccagg aggca 515 161 936 DNA Homo sapiens 161taatttctta gtcgtttgga atccttaagc atgcaaaagc tttgaacaga agggttcaca 60aaggaaccag ggttgtctta tggcatccag ttaagccaga gctgggaatg cctctgggtc 120atccacatca ggagcagaag cacttgactt gtcggtcctg ctgccacggt ttgggcgccc 180accacgccca cgtccacctc gtcctcccct gccgccacgt cctgggcggc caaggtctcc 240aaaattgatc tccagctgag acgttatatc atttgctggc ttccggaaat gatggtccat 300aaccgaatct tcagcatgag cctcttcact ctttgattta tgaagaacaa atcccttctt 360ccactgccca tcagcacctt catttggttt tcggatatta aattctactt ttgcccggtc 420cttattttga atagccttcc actcatccaa agtcatctct tttggaccct cctcttttac 480ctcttcaact tcattctcct tattttcagt gtctgccact ggatgatgtt cttcaccttc 540aggtgtttcc tcagtcacat ttgattgatc caagtcagtt aattcgtctt tgacagttcc 600ccagttgtga gatccgctac ctccacgttt gtcctcgtgc ttcaggccag atctatcact 660tccactatgc ctatcaaatt cacgtttgcc acgagaatca aatccatctc ctcggcccat 720tccacgtcca cggccccctc gacctcttcc aagaccacca cgacctcgaa taggtcggtc 780aataatcggt ctatcaactg aaaattcgcc tccttcaccc ttttcttcaa gtggcttttc 840gaatcttcgt tcacgaggtg gtcgcctttc tggtcttcta tcaattattt tcccttcacc 900ctgaagttgt tgatcaggtc ttcttccaac tcgtgc 936 162 950 DNA Homo sapiens 162aagcggatgg acctgagtca gccgaatcct agccccttcc cttgggcctg ctgtggtgct 60cgacatcagt gacagacgga agcagcagac catcaaggct acgggaggcc cggggcgctt 120gcgaagatga agtttggctg cctctccttc cggcagcctt atgctggctt tgtcttaaat 180ggaatcaaga ctgtggagac gcgctggcgt cctctgctga gcagccagcg gaactgtacc 240atcgccgtcc acattgctca cagggactgg gaaggcgatg cctgtcggga gctgctggtg 300gagagactcg ggatgactcc tgctcagatt caggccttgc tcaggaaagg ggaaaagttt 360ggtcgaggag tgatagcggg actcgttgac attggggaaa ctttgcaatg ccccgaagac 420ttaactcccg atgaggttgt ggaactagaa aatcaagctg cactgaccaa cctgaagcag 480aagtacctga ctgtgatttc aaaccccagg tggttactgg agcccatacc taggaaagga 540ggcaaggatg tattccaggt agacatccca gagcacctga tccctttggg gcatgaagtg 600tgacaagtgt gggctcctga aaggaatgtt ccrgagaaac cagctaaatc atggcacctt 660caatttgcca tcgtgacgca gacctgtata aattaggtta aagatgaatt tccactgctt 720tggagagtcc cacccactaa gcactgtgca tgtaaacagg ttcctttgct cagatgaagg 780aagtaggggg tggggctttc cttgtgtgat gcctccttag gcacacaggc aatgtctcaa 840gtactttgac cttagggtag aaggcaaagc tgccagtaaa tgtctcagca ttgctgctaa 900ttttggtcct gctagtttct ggattgtaca aataaatgtg ttgtagatga 950 163 475 DNA Homo sapiens modified_base (301) Where n is a, c, g or t 163tcgagcggcc gcccgggcag gtgtcggagt ccagcacggg aggcgtggtc ttgtagttgt 60tctccggctg cccattgctc tcccactcca cggcgatgtc gctgggatag aagcctttga 120ccaggcaggt caggctgacc tggttcttgg tcatctcctc ccgggatggg ggcagggtgt 180acacctgtgg ttctcggggc tgccctttgg ctttggagat ggttttctcg atgggggctg 240ggagggcttt gttggagacc ttgcacttgt actccttgcc attcaaccag tcctggtgca 300ngacggtgag gacgctnacc acacggtacg ngctggtgta ctgctcctcc cgcggctttg 360tcttggcatt atgcacctcc acgccgtcca cgtaccaatt gaacttgacc tcagggtctt 420cgtggctcac gtccaccacc acgcatgtaa cctcaaanct cggncgcgan cacgc 475 164 476 DNA Homo sapiens 164agcgtggtcg cggccgaggt ctgaggttac atgcgtggtg gtggacgtga gccacgaaga 60ccctgaggtc aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa 120gccgcgggag gagcagtaca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca 180ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc 240ccccatcgag aaaaccatct ccaaagccaa agggcagccc cgagaaccac aggtgtacac 300cctgccccca tcccgggagg agatgaccaa gaaccaggtc agcctgacct gcctggtcaa 360aggcttctat cccagcgaca tcgcccgtgg agtgggagag caatgggcag ccggagaaca 420actacaagac cacgcctccc gtgctggact ccgacacctg ccgggcggcc gctcga 476 165 256 DNA Homo sapiens modified_base (10) Where n is a, c, g or t 165agcgtggttn cggccgaggt cccaaccaag gctgcancct ggatgccatc aaagtcttct 60gcaacatgga gactggtgag acctgcgtgt accccactca gcccagtgtg gcccagaaga 120actggtacat cagcaagaac cccaaggaca agaggcatgt ctggttcggc gagagcatga 180ccgatggatt ccagttcgag tatggcggcc agggctccga ccctgccgat gtggacctgc 240ccgggcggnc gctcga 256 166 332 DNA Homo sapiens 166agcgtggtcg cggccgaggt caagaacccc gcccgcacct gccgtgacct caagatgtgc 60cactctgact ggaagagtgg agagtactgg attgacccca accaaggctg caacctggat 120gccatcaaag tcttctgcaa catggagact ggtgagacct gcgtgtaccc cactcagccc 180agtgtggccc agaagaactg gtacatcagc aagaacccca aggacaagag gcatgtctgg 240ttcggcgaga gcatgaccga tggattccag ttcgagtatg gcggccaggg ctccgaccct 300gccgatgtgg acctgcccgg gcggccgctc ga 332 167 332 DNA Homo sapiens modified_base (77) Where n is a, c, g or t 167tcgagcggtc gcccgggcag gtccacatcg gcagggtcgg agccctggcc gccatactcg 60aactggaatc catcggncat gctctcgccg aaccagacat gcctcttgnc cttggggttc 120ttgctgatgt accagntctt ctgggccaca ctgggctgag tggggtacac gcaggtctca 180ccantctcca tgttgcanaa gactttgatg gcatccaggt tgcagccttg gttggggtca 240atccagtact ctccactctt ccagacagag tggcacatct tgaggtcacg gcaggtgcgg 300gcggggttct tgacctcggt cgcgaccacg ct 332 168 276 DNA Homo sapiens modified_base (72) Where n is a, c, g or t 168tcgagcggcc gcccgggcag gtcctcctca gagcggtagc tgttcttatt gccccggcag 60cctccataga tnaagttatt gcangagttc ctctccacgt caaagtacca gcgtgggaag 120gatgcacggc aaggcccagt gactgcgttg gcggtgcagt attcttcata gttgaacata 180tcgctggagt ggacttcaga atcctgcctt ctgggagcac ttgggacaga ggaatccgct 240gcattcctgc tggtggacct cggccgcgac cacgct 276 169 276 DNA Homo sapiens 169agcgtggtcg cggccgaggt ccaccagcag gaatgcagcg gattcctctg tcccaagtgc 60tcccagaagg caggattctg aagaccactc cagcgatatg ttcaactatg aagaatactg 120caccgccaac gcagtcactg ggccttgccg tgcatccttc ccacgctggt actttgacgt 180ggagaggaac tcctgcaata acttcatcta tggaggctgc cggggcaata agaacagcta 240ccgctctgag gaggacctgc ccgggcggcc gctcga 276 170 332 DNA Homo sapiens modified_base (294) Where n is a, c, g or t 170tcgagcggcc gcccgggcag gtccacatcg gcagggtcgg agccctggcc gccatactcg 60aactggaatc catcggtcat gctctcgccg aaccagacat gcctcttgtc cttggggttc 120ttgctgatgt accagttctt ctgggccaca ctgggctgag tggggtacac gcaggtctca 180ccagtctcca tgttgcagaa gactttgatg gcatccaggt tgcagccttg gttggggtca 240atccagtact ctccactctt ccagccagaa tggcacatct tgaggtcacg gcangtgcgg 300gcggggttct tgacctcggc cgcgaccacg ct 332 171 333 DNA Homo sapiens 171agcgtggtcg cggccgaggt caagaaaccc cgcccgcacc tgccgtgacc tcaagatgtg 60ccactctggc tggaagagtg gagagtactg gattgacccc aaccaaggct gcaacctgga 120tgccatcaaa gtcttctgca acatggagac tggtgagacc tgcgtgtacc ccactcagcc 180cagtgtggcc cagaagaact ggtacatcag caagaacccc aaggacaaga ggcatgtctg 240gctcggcgag agcatgaccg atggattcca gttcgagtat ggcggccagg gctccgaccc 300tgccgatgtg gacctgcccg ggcggccgct cga 333 172 527 DNA Homo sapiens modified_base (46) Where n is a, c, g or t 172agcgtggtcg cggccgaggt cctgtcagag tggcactggt agaagntcca ggaaccctga 60actgtaaggg ttcttcatca gtgccaacag gatgacatga aatgatgtac tcagaagtgt 120cctgnaatgg ggcccatgan atggttgnct gagagagagc ttcttgtcct acattcggcg 180ggtatggtct tggcctatgc cttatggggg tggccgttgn gggcggtgng gtccgcctaa 240aaccatgttc ctcaaagatc atttgttgcc caacactggg ttgctgacca naagtgccag 300gaagctgaat accatttcca gtgtcatacc cagggtgggt gacgaaaggg gtcttttgaa 360ctgtggaagg aacatccaag atctctgntc catgaagatt ggggtgtgga agggttacca 420gttggggaag ctcgctgtct ttttccttcc aatcangggc tcgctcttct gaatattctt 480cagggcaatg acataaattg tatattcggt tcccggttcc aggccag 527 173 635 DNA Homo sapiens modified_base (444) Where n is a, c, g or t 173tcgagcggcc gcccgggcag gtccaccaca cccaattcct tgctggtatc atggcagccg 60ccacgtgcca ggattaccgg ctacatcatc aagtatgaga agcctgggtc tcctcccaga 120gaagtggtcc ctcggccccg ccctggtgtc acagaggcta ctattactgg cctggaaccg 180ggaaccgaat atacaattta tgtcattgcc ctgaagaata atcagaagag cgagcccctg 240attggaagga aaaagacaga cgagcttccc caactggtaa cccttccaca ccccaatctt 300catggaccag agatcttgga tgttccttcc acagttcaaa agaccccttt cgtcacccac 360cctgggtatg acactggaaa tggtattcag cttcctggca cttctggtca gcaacccagt 420gttgggcaac aaatgatctt tgangaacat ggntttaggc ggaccacacc ggccacaacg 480ggcaccccca taaggcatag gccaagaaca tacccgncga atgtaggaca agaagctctn 540tctcanacaa ncatctcatg ggccccattc cangacactt ctgagtacat canttcatgg 600catcctggtg gcactgataa aaacccttac agtta 635 174 572 DNA Homo sapiens modified_base (457) Where n is a, c, g or t 174agcgtggtcg cgggcgaggt cctgtcagag tggcactggt agaagttcca ggaaccctga 60actgtaaggg ttcttcatca gtgccaacag gatgacatga aatgatgtac tcagaagtgt 120cctggaatgg ggcccatgag atggttgtct gagagagagc ttcttgtcct acattcggcg 180ggtatggtct tggcctatgc cttatggggg tggccgttgt gggcggtgtg gtccgcctaa 240aaccatgttc ctcaaagatc atttgttgcc caacactggg ttgctgacca gaagtgccag 300gaagctgaat accatttcca gtgtcatacc cagggtgggt gacgaaaggg gtcttttgaa 360ctgtggaagg aacatccaag atctctggtc catgaagatt ggggtgtgga agggttacca 420gttggggaag ctcgtctgtc tttttccttc caatcanggg ctcgctcttc tgattattct 480tcagggcaat gacataaatt gtatattcgg ntcccgggtn cagccaataa taataaccct 540ctgtgacacc anggcggggc cgaagganca ct 572 175 372 DNA Homo sapiens modified_base (247) Where n is a, c, g or t 175agcgtggtcg cggccgaggt cctcaccaga ggtaccacct acaacatcat agtggaggca 60ctgaaagacc agcagaggca taaggttcgg gaagaggttg ttaccgtggg caactctgtc 120aacgaaggct tgaaccaacc tacggatgac tcgtgctttg acccctacac agtttcccat 180tatgccgttg gagatgagtg ggaacgaatg tctgaatcag gctttaaact gttgtgccag 240tgcttangct ttggaagtgg tcatttcaga tgtgattcat ctagatggtg ccatgacaat 300ggtgtgaact acaagattgg agagaagtgg gaccgtcagg gagaaaatgg acctgcccgg 360gcggccgctc ga 372 176 372 DNA Homo sapiens modified_base (251) Where n is a, c, g or t 176tcgagcggcc gcccgggcag gtccattttc tccctgacgg tcccacttct ctccaatctt 60gtagttcaca ccattgtcat ggcaccatct agatgaatca catctgaaat gaccacttcc 120aaagcctaag cactggcaca acagtttaaa gcctgattca gacattcgtt cccactcatc 180tccaacggca taatgggaaa ctgtgtaggg gtcaaagcac gagtcatccg taggttggtt 240caagccttcg ntgacagagt tgcccacggt aacaacctct tcccgaacct tatgcctctg 300ctggtctttc agtgcctcca ctatgatgtt gtaggtggta cctctggtga ggacctcggc 360cgcgaccacg ct 372 177 269 DNA Homo sapiens modified_base (94) Where n is a, c, g or t 177agcgtggccg cggccgaggt ccattggctg gaacggcatc aacttggaag ccagtgatcg 60tctcagcctt ggttctccag ctaatggtga tggnggtctc agtagcatct gtcacacgag 120cccttcttgg tgggctgaca ttctccagag tggtgacaac accctgagct ggtctgcttg 180tcaaagtgtc cttaagagca tagacactca cttcatattt ggcgnccacc ataagtcctg 240atacaaccac ggaatgacct gtcaggaac 269 178 529 DNA Homo sapiens 178tcgagcggcc gcccgggcag gtcctcagac cgggttctga gtacacagtc agtgtggttg 60ccttgcacga tgatatggag agccagcccc tgattggaac ccagtccaca gctattcctg 120caccaactga cctgaagttc actcaggtca cacccacaag cctgagcgcc cagtggacac 180cacccaatgt tcagctcact ggatatcgag tgcgggtgac ccccaaggag aagaccggac 240caatgaaaga aatcaacctt gctcctgaca gctcatccgt ggttgtatca ggacttatgg 300cggccaccaa atatgaagtg agtgtctatg ctcttaagga cactttgaca agcagaccag 360ctcagggtgt tgtcaccact ctggagaatg tcagcccacc aagaagggct cgtgtgacag 420atgctactga gaccaccatc accattagct ggagaaccaa gactgagacg atcactggct 480tccaagttga tgccgttcca gccaatggac ctcggccgcg accacgctt 529 179 454 DNA Homo sapiens modified_base (64) Where n is a, c, g or t 179agcgtggtcg cggccgaggt ctggccgaac tgccagtgta cagggaagat gtacatgtta 60tagntcttct cgaagtcccg ggccagcagc tccacggggt ggtctcctgc ctccaggcgc 120ttctcattct catggatctt cttcacccgc agcttctgct tctcagtcag aaggttgttg 180tcctcatccc tctcatacag ggtgaccagg acgttcttga gccagtcccg catgcgcagg 240gggaattcgg tcagctcaga gtccaggcaa ggggggatgt atttgcaagg cccgatgtag 300tccaagtgga gcttgtggcc cttcttggtg ccctccaagg tgcactttgt ggcaaagaag 360tggcaggaag agtcgaaggt cttgttgtca ttgctgcaca ccttctcaaa ctcgccaatg 420ggggctgggc agacctgccc gggcggccgc tcga 454 180 454 DNA Homo sapiens modified_base (55) Where n is a, c, g or t 180tcgagcggcc gcccgggcag gtctgcccag cccccattgg cgagtttgag aaggngtgca 60gcaatgacaa caagaccttc gactcttcct gccacttctt tgccacaaag tgcaccctgg 120agggcaccaa gaagggccac aagctccacc tggactacat cgggccttgc aaatacatcc 180ccccttgcct ggactctgag ctgaccgaat tccccctgcg catgcgggac tggctcaaga 240acgtcctggt caccctgtat gagagggatg aggacaacaa ccttctgact gagaagcana 300agctgcgggt gaagaanatc catgagaatg anaagcgcct gnaggcanga gaccaccccg 360tggagctgct ggcccgggac ttcgagaaga actataacat gtacatcttc cctgtacact 420ggcagttcgg ccagacctcg gccgcgacca cgct 454 181 102 DNA Homo sapiens modified_base (8) Where n is a, c, g or t 181agcgtggntg cggacgacgc ccacaaagcc attgtatgta gttttanttc agctgcaaan 60aataccncca gcatccacct tactaaccag catatgcaga ca 102 182 337 DNA Homo sapiens modified_base (169) Where n is a, c, g or t 182tcgagcggtc gcccgggcag gtctgggcgg atagcaccgg gcatattttg gaatggatga 60ggtctggcac cctgagcagc ccagcgagga cttggtctta gttgagcaat ttggctagga 120ggatagtatg cagcacggtt ctgagtctgt gggatagctg ccatgaagna acctgaagga 180ggcgctggct ggtangggtt gattacaggg ctgggaacag ctcgtacact tgccattctc 240tgcatatact ggntagtgag gcgagcctgg cgctcttctt tgcgctgagc taaagctaca 300tacaatggct ttgnggacct cggccgcgac cacgctt 337 183 374 DNA Homo sapiens 183tcgagcggcc gcccgggcag gtccattttc tccctgacgg tcccacttct ctccaatctt 60gtagttcaca ccattgtcat gacaccatct agatgaatca catctgaaat gaccacttcc 120aaagcctaag cactggcaca acagtttaaa gcctgattca gacattcgtt cccactcatc 180tccaacggca taatgggaaa ctgtgtaggg gtcaaagcac gagtcatccg taggttggtt 240caagccttcg ttgacagaag ttgcccacgg taacaacctc ttcccgaacc ttatgcctct 300gctggtcttt caagtgcctc cactatgatg ttgtaggtgg cacctctggt gaggacctcg 360gccgcgacca cgct 374 184 375 DNA Homo sapiens modified_base (30) Where n is a, c, g or t 184agcgtggttt gcggccgagg tcctcaccan aggtgccacc tacaacatca tagtggaggc 60actgaaagac cagcagaggc ataaggttcg ggaagaggtt gttaccgtgg gcaactctgt 120caacgaaggc ttgaaccaac ctacggatga ctcgtgcttt gacccctaca cagnttccca 180ttatgccgtt ggagatgagt gggaacgaat gtctgaatca ggctttaaac tgttgtgcca 240gtgcttangc tttggaagtg gtcatttcag atgtgattca tctanatggt gtcatgacaa 300tggtgngaac tacaagattg gagagaagtg gnaccgtcag ggganaaaat ggacctgccc 360gggcggcncg ctcga 375 185 148 DNA Homo sapiens modified_base (28) Where n is a, c, g or t 185agcgtggtcg cggccgaggt ctggcttnct gctcangtga ttatcctgaa ccatccaggc 60caaataagcg ccggctatgc ccctgnattg gattgccaca cggctcacat tgcatgcaag 120tttgctgagc tgaaggaaaa gattgatc 148 186 397 DNA Homo sapiens modified_base (78) Where n is a, c, g or t 186tcgagcggcc gcccgggcag gtccaattga aacaaacagt tctgagaccg ttcttccacc 60actgattaag agtggggngg cgggtattag ggataatatt catttagcct tctgagcttt 120ctgggcagac ttggtgacct tgccagctcc agcagccttc tggtccactg ctttgatgac 180acccaccgca actgtctgtc tcatatcacg aacagcaaag cgacccaaag gtggatagtc 240tgagaagctc tcaacacaca tgggcttgcc aggaaccata tcaacaatgg gcagcatcac 300cagacttcaa gaatttaagg gccatcttcc agctttttac cagaacggcg atcaatcttt 360tccttcagct cagcaaactt gcatgcaatg tgagccg 397 187 584 DNA Homo sapiens modified_base (145) Where n is a, c, g or t 187tcgagcggcc gcccgggcag gtccagaggg ctgtgctgaa gtttgctgct gccactggag 60ccactccaat tgctggccgc ttcactcctg gaaccttcac taaccagatc caggcagcct 120tccgggagcc acggcttctt gtggntactg accccagggc tgaccaccag cctctcacgg 180aggcatctta tgttaaccta cctaccattg cgctgtgtaa cacagattct cctctgcgct 240atgtggacat tgccatccca tgcaacaaca agggagctca ctcagngggg tttgatgtgg 300tggatgctgg ctcgggaagt tctgcgcatg cgtggcacca tttcccgtga acacccatgg 360gangncatgc ctgatctgga cttctacaga gatcctgaag agattgaaaa agaagaacag 420gctgnttgct ganaaagcaa gtgaccaagg angaaatttc angggtgaaa nggactgctc 480ccgctcctga attcactgct actcaacctg angntgcaga ctggtcttga aggngnacan 540gggccctctg ggcctattta agcancttcg gtcgcgaaca cgnt 584 188 579 DNA Homo sapiens modified_base (7) Where n is a, c, g or t 188agcgtgngtc gcggccgagg tgctgaatag gcacagaggg cacctgtaca ccttcagacc 60agtctgcaac ctcaggctga gtagcagtga actcaggagc gggagcagtc cattcaccct 120gaaattcctc cttggncact gccttctcag cagcagcctg ctcttctttt tcaatctctt 180caggatctct gtagaagtac agatcaggca tgacctccca tgggtgttca cgggaaatgg 240tgccacgcat gcgcagaact tcccgagcca gcatccacca catcaaaccc actgagtgag 300ctcccttgtt gttgcatggg atgggcaatg tccacatagc gcagaggaga atctgtgtta 360cacagcgcaa tggtaggtag gttaacataa gatgcctccg cgagaagctg gtggtcagcc 420ctggggtcaa gtaaccacaa gaagccgtgg ctcccggaag gctgcctgga tctggttagt 480gaaggntcca ggagtgaagc ggccaacaat tggagtggct tcagtggcaa gcagcaaact 540tcagcacaag ccctctggac ctgcccggcg gccgctcga 579 189 374 DNA Homo sapiens modified_base (41) Where n is a, c, g or t 189tcgagcggcc gcccgggcag gtccattttc tccctgacgg ncccacttct ctccaatctt 60gtagttcaca ccattgtcat ggcaccatct agatgaatca catctgaaat gaccacttcc 120aaagcctaag cactggcaca acagtttaaa gcctgattca gacattcgtt cccactcatc 180tccaacggca taatgggaaa ctgtgtaggg gtcaaagcac gagtcatccg taggttggtt 240caagccttcg ttgacagagt tgcccacggt aacaacctcn tccccgaacc ttatgcctct 300gctgggcttt cagngcctcc actatgatgn tgtagggggg cacctctggn gangacctcg 360gccgcgacca cgct 374 190 373 DNA Homo sapiens modified_base (247) Where n is a, c, g or t 190agcgtggtcg cggccgaggt cctcaccaga ggtgccacct acaacatcat agtggaggca 60ctgaaagacc agcagaggca taaggctcgg gaagaggttg ttaccgtggg caactctgtc 120aacgaaggct tgaaccaacc tacggatgac tcgtgctttg acccctacac agtttcccat 180tatgccgttg gagatgagtg ggaacgaatg tctgaatcag gctttaaact gttgtgccag 240tgcttangct ttggaagtgg gtcatttcag atgtgattca tctagatggt gccatgacaa 300tggngngaac tacaagattg gagagaagtg gnaccgncag ggagaaaatg gacctgcccg 360ggcggccgct cga 373 191 354 DNA Homo sapiens modified_base (218) Where n is a, c, g or t 191agcgtggtcg cggccgaggt ccacatcggc agggtcggag ccctggccgc catactcgaa 60ctggaatcca tcggtcatgc tctcgccgaa ccagacatgc ctcttgtcct tggggttctt 120gctgatgtac cagttcttct gggccacact gggctgagtg gggtacacgc aggtctcacc 180agtctccatg ttgcagaaga ctttgatggc atccaggntg caaccttggt tggggtcaat 240ccagtactct ccactcttcc agccagagtg gcacatcttg aggtcacggc aggtgcggnc 300gggggntttt gcggctgccc tctggncttc ggntgtnctc natctgctgg ctca 354 192 587 DNA Homo sapiens modified_base (276) Where n is a, c, g or t 192tcgagcggcc gcccgggcag gtctcgcggt cgcactggtg atgctggtcc tgttggtccc 60cccggccctc ctggacctcc tggcccccct ggtcctccca gcgctggttt cgacttcagc 120ttcctgcccc agccacctca agagaaggct cacgatggtg gccgctacta ccgggctgat 180gatgccaatg tggttcgtga ccgtgacctc gaggtggaca ccaccctcaa gagcctgagc 240cagcagatcg agaacatccg gagcccagag ggcagncgca agaaccccgc ccgcacctgc 300cgtgacctca agatgtgcca ctctgactgg aagagtggag agtactggat tgaccccaac 360caagctgcaa cctggatgcc atcaaagtct tctgcaacat ggagactggt gagacctgcg 420tgtaccccac tcagcccagt gtggcccaaa agaactggta catcagcaag aaccccaagg 480acaagaagca tgtctggttc ggcgagaaca tgaccgatgg attccagttc gagtatggcg 540ggcagggctc cgaccctgcc gatggggacc ttggccgcga acacgct 587 193 98 DNA Homo sapiens modified_base (8) Where n is a, c, g or t 193agcgtggnng cggccgaggt ataaatatcc agnccatatc ctccctccac acgctganag 60atgaagctgt ncaaagatct cagggtggan aaaaccat 98 194 240 DNA Homo sapiens 194tcgagcggcc gcccgggcag gtccttcaga cttggactgt gtcacactgc caggcttcca 60gggctccaac ttgcagacgg cctgttgtgg gacagtctct gtaatcgcga aagcaaccat 120ggaagacctg ggggaaaaca ccatggtttt atccaccctg agatctttga acaacttcat 180ctctcagcgt gcggagggag gctctggact ggatatttct acctcggccg cgaccacgct 240 195 400 DNA Homo sapiens modified_base (22) Where n is a, c, g or t 195cgagcgggcg accgggcagg tncagactcc aatccanana accatcaagc cagatgtcag 60aagctacacc atcacaggtt tacaaccagg cactgactac aaganctacc tgcacacctt 120gaatgacaat gctcggagct cccctgtggt catcgacgcc tccactgcca ttgatgcacc 180atccaacctg cgtttcctgg ccaccacacc caattccttg ctggtatcat ggcagccgcc 240acgtgccagg attaccggta catcatcnag tatganaagc ctgggcctcc tcccagagaa 300gnggtccctc ggccccgccc tgntgtccca naggntacta ttactgngcc ngcaaccggc 360aaccgatatc nattttgnca ttggccttca acaataatta 400 196 494 DNA Homo sapiens modified_base (19) Where n is a, c, g or t 196agcgtggttc gcggccgang tcctgtcaga gtggcactgg tagaagttcc aggaaccctg 60aactgtaagg gttcttcatc agngccaaca ggatgacatg aaatgatgta ctcagaagtg 120tcctggaatg gggcccatga gatggttgtc tgagagagag cttcttgncc tgtctttttc 180cttccaatca ggggctcgct cttctgatta ttcttcaggg caatgacata aattgtatat 240tcgggtcccg gntccaggcc agtaatagta ncctctgtga caccagggcg gngccgaggg 300accacttctc tgggaggaga cccaggcttc tcatacttga tgatgtaacc ggtaatcctg 360gcacgtggcg gctgccatga taccagcaag gaattggggt gtggtggcca ggaaacgcag 420gttggatggn gcatcaatgg cagtggaggc cgtcgatgac cacaggggga gctccgacat 480tgtcattcaa ggtg 494 197 118 DNA Homo sapiens modified_base (8) Where n is a, c, g or t 197agcgtggncg cggccgaggt gcagcgcggg ctgtgccacc ttctgctctc tgcccaacga 60taaggagggt ncctgccccc aggagaacat taactntccc cagctcggcc tctgccgg 118 198 403 DNA Homo sapiens modified_base (41) Where n is a, c, g or t 198tcgagcggcc gcccgggcag gttttttttg ctgaaagtgg ntactttatt ggntgggaaa 60gggagaagct gtggtcagcc caagagggaa tacagagncc cgaaaaaggg gagggcaggt 120gggctggaac cagacgcagg gccaggcaga aactttctct cctcactgct cagcctggtg 180gtggctggag ctcanaaatt gggagtgaca caggacacct tcccacagcc attgcggcgg 240catttcatct ggccaggaca ctggctgtcc acctggcact ggtcccgaca gaagcccgag 300ctggggaaag ttaatgttca cctgggggca ggaaccctcc ttatcattgn gcagagagca 360gaaggtggca cagcccgcgc tgcacctcgg ccgcgaccac gct 403 199 167 DNA Homo sapiens modified_base (92) Where n is a, c, g or t 199tcgagcggcc gcccgggcag gtccaccata agtcctgata caaccacgga tgagctgtca 60ggagcaaggt tgatttcttt cattggtccg gncttctcct tgggggncac ccgcactcga 120tatccagtga gctgaacatt gggtggcgtc cactgggcgc tcaggct 167 200 252 DNA Homo sapiens modified_base (210) Where n is a, c, g or t 200tcgagcggtt cgcccgggca ggtccaccac acccaattcc ttgctggtat catggcagcc 60gccacgtgcc aggattaccg gctacatcat caagtatgag aagcctgggt ctcctcccag 120agaagcggtc cctcggcccc gccctggtgt cacagaggct actattactg gcctggaacc 180gggaaccgaa tatacaattt atgtcattgn cctgaagaat aatcannaan agcgancccc 240tgattggaag ga 252 201 91 DNA Homo sapiens 201agcgtggtcg cggccgaggt tgtacaagct tttttttttt tttttttttt tttttttttt 60tttttttttt tttttttttt tttttttttt t 91 202 368 DNA Homo sapiens modified_base (9) Where n is a, c, g or t 202tcgagcggnc gcccgggcag gtctgccaac accaagattg gcccccgccg catccacaca 60gtccgtgtgc ggggaggtaa caagaaatac cgtgccctga ggttggacgt ggggaatttc 120tcctggggct cagagtgttg tactcgtaaa acaaggatca tcgatgttgt ctacaatgca 180tctaataacg agctggttcg taccaagacc ctggtgaaga attgcatcgt gctcatcgac 240agcacaccgt accgacagtg gtacgagtcc cactatgcgc tgcccctggg ccgcaagaag 300ggagccaagc tgactcctga ggaagaagag attttaaaca aaaaacgatc taanaaaaaa 360aaaacaat 368 203 340 DNA Homo sapiens 203agcgtggtcg cggccgaggt gaaatggtat tcagcttcct ggcacttctg gtcagcaacc 60cagtgttggg caacaaatga tctttgagga acatggtttt aggcggacca caccgcccac 120aacggccacc cccataaggc ataggccaag accatacccg ccgaatgtag gacaagaagc 180tctctctcag acaaccatct catgggcccc attccaggac acttctgagt acatcatttc 240atgtcatcct gttggcactg atgaagaacc cttacagttc agggttcctg gaacttctac 300cagtgccact ctgacaggac ctgcccgggc ggccgctcga 340 204 341 DNA Homo sapiens 204tcgagcggcc gcccgggcag gtcctgtcag agtggcactg gtagaagttc caggaaccct 60gaactgtaag ggttcttcat cagtgccaac aggatgacat gaaatgatgt actcagaagt 120gtcctggaat ggggcccatg agatggttgt ctgagagaga gcttcttgtc ctacattcgg 180cgggtatggt cttggcctat gccttatggg ggtggccgtt gtgggcggtg tggtccgcct 240aaaaccatgt tcctcaaaga tcatttgttg cccaacactg ggttgctgac cagaagtgcc 300aggaagctga ataccatttc acctcggccg cgaccacgct a 341 205 770 DNA Homo sapiens modified_base (529) Where n is a, c, g or t 205tcgagcggcc gcccgggcag gtctcccttc ttgcggccca ggggcagcgc atagtgggac 60tcgtaccact gtcggtacgg tgtgctgtcg atgagcacga tgcaattctt caccagggtc 120ttggtacgaa ccagctcgtt attagatgca ttgtagacaa catcgatgat ccttgtttta 180cgagtacaac actctgagcc ccaggagaaa ttccccacgt ccaacctcag ggcacggtat 240ttcttgttac ctccccgcac acggactgtg tggatgcggc gggggccaag ctgactcctg 300aggaagaaga gattttaaac aaaaaacgat ctaaaaaaat tcagaagaaa tatgatgaaa 360ggaaaaagaa tgccaaaatc agcagtctcc tggaggagca gttccagcag ggcaagcttc 420ttgcgtgcat cgcttcaagg ccgggacagt gtgaccgagc agatggctat gtgctagagg 480gcaaagaagt ggagttctat cttaagaaaa tcagggccca gaatggtgng tcttcaacta 540atccaaaggg gagtttcaga ccagtgcaat cagcaaaaac attgatactg ntggccaaat 600ttattggtgc agggcttgca cantangann ggctgggtct tggggcttgg attggnacaa 660gctttggcag ccttttcttt ggttttgcca aaaacctttt gntgaagang anacctnggg 720cggacccctt aaccgattcc acnccnggng gcgttctang gncccncttg 770 206 810 DNA Homo sapiens modified_base (574) Where n is a, c, g or t 206agcgtggtcg cggccgaggt ctgctgcttc agcgaagggt ttctggcata accaatgata 60aggctgccaa agactgttcc aataccagca ccagaaccag ccactcctac tgttgcagca 120cctgcaccaa taaatttggc agcagtatca atgtctctgc tgattgcact ggtctgaaac 180tccctttgga ttagctgaga cacaccattc tgggccctga ttttcctaag atagaactcc 240aactctttgc cctctagcac atagccatct gctcggtcac actgtcccgg ccttgaagcg 300atgcacgcaa gaagcttgcc ctgctggaac tgctcctcca ggagactgct gattttggca 360ttctttttcc tttcatcata tttcttctga atttttttag atcgtttttt gtttaaaatc 420tcttcttcct caggagtcag cttggccccc gccgcatcca cacagtccgt gtgcggggag 480gtaacaagaa ataccgtgcc ctgaggttgg acgtggggaa tttctcctgg ggctcagagt 540ggtgtactcg taaaacaagg atcatcgatg gtgnctacaa tgcatctaat aacgagctgg 600gtcggaccca aagaacctgg ngaanaaatg gatcgnctca tcgacaggac accgtacccg 660acaggggnac gantcccact atgcgcttgc ccctgggccg caanaaagga aaactgcccg 720ggcggccntc gaaagcccaa ttntggaaaa aatccatcac actgggnggc cngtcgagca 780tgcatntana ggggcccatt ccccctnann 810 207 257 DNA Homo sapiens 207tcgagcggcc gcccgggcag gtccccaacc aaggctgcaa cctggatgcc atcaaagtct 60tctgcaacat ggagactggt gagacctgcg tgtaccccac tcagcccagt gtggcccaga 120agaactggta catcagcaag aaccccaagg acaagaggca tgtctggttc ggcgagagca 180tgaccgatgg attccagttc gagtatggcg gccagggctc cgaccctgcc gatgtggacc 240tcggccgcga ccacgct 257 208 257 DNA Homo sapiens 208agcgtggtcg cggccgaggt ccacatcggc agggtcggag ccctggccgc catactcgaa 60ctggaatcca tcggtcatgc tctcgccgaa ccagacatgc ctcttgtcct tggggttctt 120gctgatgtac cagttcttct gggccacact gggctgagtg gggtacacgc aggtctcacc 180agtctccatg ttgcagaaga ctttgatggc atccaggttg cagccttggt tggggacctg 240cccgggcggc cgctcga 257 209 747 DNA Homo sapiens modified_base (453) Where n is a, c, g or t 209tcgagcggcc gcccgggcag gtccaccaca cccaattcct tgctggtatc atggcagccg 60ccacgtgcca ggattaccgg ctacatcatc aagtatgaga agcctgggtc tcctcccaga 120gaagtggtcc ctcggccccg ccctggtgtc acagaggcta ctattactgg cctggaaccg 180ggaaccgaat atacaattta tgtcattgcc ctgaagaata atcagaagag cgagcccctg 240attggaagga aaaagacaga cgagcttccc caactggtaa cccttccaca ccccaatctt 300catggaccag agatcttgga tgttccttcc acagttcaaa agaccccttt cgtcacccac 360cctgggtatg acactggaaa tggtattcag cttcctggca cttctggtca gcaacccagt 420gttgggcaac aaatgatctt tgaggaacat ggntttaggc ggaccacacc gcccacaacg 480gccaccccca taaggcatag gccaagacca tacccgccga atgtaggaca agaagctntn 540tntcanacac catntnatgg gccccattcc aggacacttc tgagtacatc atttatgnca 600tctgtggcac ttgatgaaaa cccttacagt tcagggttct ggaactttta ccaggcctnt 660tacaggactn ggccggacnc cttaagccna ttncaccctg gggcgttcta nggtcccact 720cgnncactgg ngaaaatggc tactgtn 747 210 872 DNA Homo sapiens modified_base (165) Where n is a, c, g or t 210agcgtggtcg cggccgaggt ccactagagg tctgtgtgcc attgcccagg cagagtctct 60gcgttacaaa ctcctaggag ggcttgctgt gcggagggcc tgctatggtg tgctgcggtt 120catcatggag agtggggcca aaggctgcga ggttgtggtg tctgngaaac tccnaggaca 180ngagggctaa attccatgaa gtttgtggat ggcctgatga tccacaatcg gagaccctgt 240taactactac cgtctnaccn cctgctgtnc ncccccnttt ctgctnaana catngggntn 300ntncttgncc ntccttgggt ngaanatnna atngcctncc cnttcntanc nctactngnt 360ccananttgg cctttaaana atccnccttg ccttnnncac tgttcanntn tttnntcgta 420aaccctatna nttnnattan atnntnnnnn nctcaccccc ctcntcattn anccnatang 480ctnnnaantc cttnanncct cccncccnnt ncnctcntac tnantncttc tnncccatta 540cnnagctctt tcntttaana taatgnngcc nngctctnca tntctacnat ntgnnnaatn 600cccccncccc cnancgnntt tttgacctnn naacctcctt tcctcttccc tncnnaaatt 660ncnnanttcc ncnttccnnc ntttcggntn ntcccatnct ttccannnct tcantctanc 720ncnctncaac ttattttcct ntcatccctt nttctttaca nnccccctnn tctactcnnc 780nnttncatta natttgaaac tnccacnnct anttncctcn ctctacnntt ttattttncg 840ntcnctctac ntaatanttt aatnanttnt cn 872 211 517 DNA Homo sapiens modified_base (462) Where n is a, c, g or t 211tcgagcggcc gcccgggcag gtctgccaag gagaccctgt tatgctgtgg ggactggctg 60gggcatggca ggcggctctg gcttcccacc cttctgttct gagatggggg tggtgggcag 120tatctcatct ttgggttcca caatgctcac gtggtcaggc aggggcttct tagggccaat 180cttaccagtt gggtcccagg gcagcatgat cttcaccttg atgcccagca caccctgtct 240gagcaacacg tggcgcacaa gcagtgtcaa cgtagtaagt taacagggtc tccgctgtgg 300atcatcaggc catccacaaa cttcatggat ttagccctct gtcctcggag tttcccagac 360accacaacct cgcagccttt ggccccactc tccatgatga accgcagcac accatagcag 420gccctccgca caagcaagcc ctcctaagaa tttgtaacgc ananactctg ctggcaatgg 480cacacaaacc tctagtggac ctcggncgcg accacgc 517 212 695 DNA Homo sapiens modified_base (432) Where n is a, c, g or t 212tcgagcggcc gcccgggcag gtctggtcca ggatagcctg cgagtcctcc tactgctact 60ccagacttga catcatatga atcatactgg ggagaatagt tctgaggacc agtagggcat 120gattcacaga ttccaggggg gccaggagaa ccaggggacc ctggttgtcc tggaatacca 180gggtcaccat ttctcccagg aataccagga gggcctggat ctcccttggg gccttgaggt 240ccttgaccat taggagggcg agtaggagca gttggaggct gtgggcaaac tgcacaacat 300tctccaaatg gaatttctgg gttggggcag tctaattctt gatccgtcac atattatgtc 360atcgcagaga acggatcctg agtcacagac acatatttgg catggttctg gcttccagac 420atctctatcc gncataggac tgaccaagat gggaacatcc tccttcaaca agcttnctgt 480tgtgccaaaa ataatagtgg gatgaagcag accgagaagt anccagctcc cctttttgca 540caaagcntca tcatgtctaa atatcagaca tgagacttct ttgggcaaaa aaggagaaaa 600agaaaaagca gttcaaagta nccnccatca agttggttcc ttgcccnttc agcacccggg 660ccccgttata aaacacctng ggccggaccc ccctt 695 213 804 DNA Homo sapiens modified_base (552) Where n is a, c, g or t 213agcgtggtcg cggccgaggt gttttatgac gggcccggtg ctgaagggca gggaacaact 60tgatggtgct actttgaact gcttttcttt tctccttttt gcacaaagag tctcatgtct 120gatatttaga catgatgagc tttgtgcaaa aggggagctg gctacttctc gctctgcttc 180atcccactat tattttggca caacaggaag ctgttgaagg aggatgttcc catcttggtc 240agtcctatgc ggatagagat gtctggaagc cagaaccatg ccaaatatgt gtctgtgact 300caggatccgt tctctgcgat gacataatat gtgacgatca agaattagac tgccccaacc 360cagaaattcc atttggagaa tgttgtgcag tttgcccaca gcctccaact gctcctactc 420gccctcctaa tggtcaagga cctcaaggcc ccaagggaga tccaggccct cctggtattc 480ctgggagaaa tggtgaccct ggtattccag gacaaccagg gtcccctggt tctcctggcc 540cccctggaat cnggngaatc atgccctact ggtcctcaaa ctattctccc anatgattca 600tatgatgtca agtctgggat agcnagtang ganggactcg caggctattc tggaccanac 660ctgccggggg ggcgttcgaa agcccgaatc tgcananntn cnttcacact ggcggccgtc 720gagctgcttt aaaagggcca ttccnccttt agngnggggg antacaatta ctnggcggcg 780ttttanancg cgngnctggg aaat 804 214 594 DNA Homo sapiens modified_base (452) Where n is a, c, g or t 214agcgtggtcg cggccgaggt ccacatcggc agggtcggag ccctggccgc catactcgaa 60ctggaatcca tcggtcatgc tctcgccgaa ccagacatgc ctcttgtcct tggggttctt 120gctgatgtac cagttcttct gggccacact gggctgagtg gggtacacgc aggtctcacc 180agtctccatg ttgcagaaga ctttgatggc atccaggttg cagccttggt tggggtcaat 240ccagtactct ccactcttcc agtcagagtg gcacatcttg aggtcacggc aggtgcgggc 300ggggttcttg cggctgccct ctgggctccg gatgttctcg atctgctggc tcaggctctt 360gagggtggtg tccacctcga ggtcacggtc acgaaccaca ttggcatcat cagcccggta 420gtagcggcca ccatcgtgag ccttctcttg angtggctgg ggcaggaact gaagtcgaaa 480ccagcgctgg gaggaccagg gggaccaana ggtccaggaa gggcccgggg gggaccaaca 540ggaccagcat caccaagtgc gacccgcgag aacctgcccg gccgnccgct cgaa 594 215 590 DNA Homo sapiens modified_base (8) Where n is a, c, g or t 215tcgagcgnnc gcccgggcag gtctcgcggt cgcactggtg atgctggtcc tgttggtccc 60cccggccctc ctggacctcc tggtccccct ggtcctccca gcgctggttt cgacttcagc 120ttcctgcccc agccacctca agagaaggct cacgatggtg gccgctacta ccgggctgat 180gatgccaatg tggttcgtga ccgtgacctc gaggtggaca ccaccctcaa gagcctgagc 240cagcagatcg agaacatccg gagcccagag ggcagccgca agaaccccgc ccgcacctgc 300cgtgacctca agatgtgcca ctctgactgg aagagtggag agtactggat tgaccccaac 360caaggctgca acctggatgc catcaaagtc ttctgcaaca tggagactgg tgagacctgc 420gtgtacccca ctcagcccag tgtggcccag aagaactggt acatcagcaa gaaccccaag 480gacaagaggc atgtctggtt cggcgagagc atgaccgatg gattccagtt cgagtatggc 540ggccagggct cccaccctgc cgatgtggac ctccggccgc gaccaccctt 590 216 801 DNA Homo sapiens modified_base (2) Where n is a, c, g or t 216tngagcggcc gcccgggcag gntgnnaacg ctggtcctgc tggtcctcct ggcaaggctg 60gtgaagatgg tcaccctgga aaacccggac gacctggtga gagaggagtt gttggaccac 120agggtgctcg tggtttccct ggaactcctg gacttcctgg cttcaaaggc attaggggac 180acaatggtct ggatggattg aagggacagc ccggtgctcc tggtgtgaag ggtgaacctg 240gtgcccctgg tgaaaatgga actccaggtc aaacaggagc ccgtgggctt cctggtgaga 300gaggaccgtg ttggtgcccc tggcccanac ctcggccgcg accacgctaa gcccgaattt 360ccagcacact ggnggccgtt actantggat ccgagctcgg taccaagctt ggcgtaatca 420tggtcatagc tgtttcctgn gtgaaattgt tatccgctca caatttcaca cancatacga 480agccggaaag cataaagtgt aaagccttgg ggtgctaatg agtgagctaa ctcncattaa 540attgcgttgc gctcactgcc cgcttttcca nnngggaaac cntggcntng ccngcttgcn 600ttaantgaaa tccgccnacc cccggggaaa agncggtttg cngtattggg gcnctttttc 660cctttcctcg gnttacttga nttantgggc tttggncgnt tcgggttgng gcgancnggt 720tcaacntcac nccaaaggng gnaanacggt tttcccanaa tccgggggnt ancccaangn 780aaaacatnng ncnaangggc t 801 217 349 DNA Homo sapiens modified_base (10) Where n is a, c, g or t 217agcgtggttn gcggccgagg tctgggccag gggcaccaac acgtcctctc tcaccaggaa 60gcccacgggc tcctgtttga cctggagttc cattttcacc aggggcacca ggttcaccct 120tcacaccagg agcaccgggc tgtcccttca atccatncag accattgtgn cccctaatgc 180ctttgaagcc aggaagtcca ggagttccag ggaaaccacc gagcaccctg tggtccaaca 240actcctctct caccaggtcg tccgggtttt ccagggtgac catcttcacc agccttgcca 300ggaggaccag caggaccagc gttaccaacc tgcccgggcg gccgctcga 349 218 372 DNA Homo sapiens 218tcgagcggcc gcccgggcag gtccattttc tccctgacgg tcccacttct ctccaatctt 60gtagttcaca ccattgtcat ggcaccatct agatgaatca catctgaaat gaccacttcc 120aaagcctaag cactggcaca acagtttaaa gcctgattca gacattcgtt cccactcatc 180tccaacggca taatgggaaa ctgtgtaggg gtcaaagcac gagtcatccg taggttggtt 240caagccttcg ttgacagagt tgcccacggt aacaacctct tcccgaacct tatgcctctg 300ctggtctttc agtgcctcca ctatgatgtt gtaggtggca cctctggtga ggacctcggc 360cgcgaccacg ct 372 219 374 DNA Homo sapiens 219agcgtggtcg cggccgaggt cctcaccaga ggtgccacct acaacatcat agtggaggca 60ctgaaagacc agcagaggca taaggttcgg gaagaggttg ttaccgtggg caactctgtc 120aacgaaggct tgaaccaacc tacggatgac tcgtgctttg acccctacac agtttcccat 180tatgccgttg gagatgagtg ggaacgaatg tctgaatcag gctttaaact gttgtgccag 240tgcttaggct ttggaagtgg tcatttcaag atgtgattca tctagatggt gccatgacaa 300tggtgtgaac tacaagattg gagagaagtg ggaccgtcag ggagaaaatg gacctgcccg 360ggccggccgc tcga 374 220 828 DNA Homo sapiens modified_base (8) Where n is a, c, g or t 220tcgagcgnnc gcccgggcag gtccagtagt gccttcggga ctgggttcac ccccaggtct 60gcggcagttg tcacagcgcc agccccgctg gcctccaaag catgtgcagg agcaaatggc 120accgagatat tccttctgcc actgttctcc tacgtggtat gtcttcccat catcgtaaca 180cgttgcctca tgagggtcac acttgaattc tccttttccg ttcccaagac atgtgcagct 240catttggctg gctctatagt ttggggaaag tttgttgaaa ctgtgccact gacctttact 300tcctccttct ctactggagc tttcgtacct tccacttctg ctgttggtaa aatggtggat 360cttctatcaa tttcattgac agtacccact tctcccaaac atccagggaa atagtgattt 420cagagcgatt aggagaacca aattatgggg cagaaataag gggcttttcc acaggttttc 480ctttggagga agatttcagt ggtgacttta aaagaatact caacagtgtc ttcatcccca 540tagcaaaaga agaaacngta aatgatggaa ngcttctgga gatgccnnca tttaagggac 600ncccagaact tcaccatcta caggacctac ttcagtttac annaagncac atantctgac 660tcanaaagga cccaagtagc nccatggnca gcactttnag cctttcccct ggggaaaann 720ttacnttctt aaancctngg ccnngacccc cttaagncca aattntggaa aanttccntn 780cnnctggggg gcngttcnac atgcntttna agggcccaat tnccccnt 828 221 476 DNA Homo sapiens 221tcgagcggcc gcccgggcag gtgtcggagt ccagcacggg aggcgtggtc ttgtagttgt 60tctccggctg cccattgctc tcccactcca cggcgatgtc gctgggatag aagcctttga 120ccaggcaggt caggctgacc tggttcttgg tcatctcctc ccgggatggg ggcagggtgt 180acacctgtgg ttctcggggc tgccctttgg ctttggagat ggttttctcg atgggggctg 240ggagggcttt gttggagacc ttgcacttgt actccttgcc attcagccag tcctggtgca 300ggacggtgag gacgctgacc acacggtacg tgctgttgta ctgctcctcc cgcggctttg 360tcttggcatt atgcacctcc acgccgtcca cgtaccagtt gaacttgacc tcagggtctt 420cgtggctcac gtccaccacc acgcatgtaa cctcagacct cggccgcgac cacgct 476 222 477 DNA Homo sapiens 222agcgtggtcg cggccgaggt ctgaggttac atgcgtggtg gtggacgtga gccacgaaga 60ccctgaggtc aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa 120gccgcgggag gagcagtaca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca 180ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc 240ccccatcgag aaaaccatct ccaaagccaa agggcaagcc ccgagaacca caggtgtaca 300ccctgccccc atcccgggag gagatgacca agaaccaggt cagcctgacc tgcctggtca 360aaggcttcta tcccagcgac atcgccgtgg agtgggagag caatgggcag ccggagaaca 420actacaagac cacgcctccc gtgctggact ccgacacctg cccgggcggc cgctcga 477 223 361 DNA Homo sapiens 223tcgagcggcc gcccgggcag gttgaatggc tcctcgctga ccaccccggt gctggtggtg 60ggtacagagc tccgatgggt gaaaccattg acatagagac tgtccctgtc cagggtgtag 120gggcccagct cagtgatgcc gtgggtcagc tggctcagct tccagtacag ccgctctctg 180tccagtccag ggcttttggg gtcaggacga tgggtgcaga cagcatccac tctggtggct 240gccccatcct tctcaggcct gagcaaggtc agtctgcaac cagagtacag agagctgaca 300ctggtgttct tgaacaaggg cataagcaga ccctgaagga cacctcggcc gcgaccacgc 360t 361 224 361 DNA Homo sapiens 224agcgtggtcg cggccgaggt gtccttcagg gtctgcttat gcccttgttc aagaacacca 60gtgtcagctc tctgtactct ggttgcagac tgaccttgct caggcctgag aaggatgggg 120cagccaccag agtggatgct gtctgcaccc atcgtcctga ccccaaaagc cctggactgg 180acagagagcg gctgtactgg aagctgagcc agctgaccca cggcatcact gagctgggcc 240cctacaccct ggacagggac agtctctatg tcaatggttt cacccatcgg agctctgtac 300ccaccaccag caccggggtg gtcagcgagg agccattcaa cctgcccggg cggccgctcg 360a 361 225 766 DNA Homo sapiens modified_base (574) Where n is a, c, g or t 225agcgtggtcg cggccgaggt cctgtcagag tggcactggt agaagttcca ggaaccctga 60actgtaaggg ttcttcatca gtgccaacag gatgacatga aatgatgtac tcagaagtgt 120cctggaatgg ggcccatgag atggttgtct gagagagagc ttcttgtcct acattcggcg 180ggtatggtct tggcctatgc cttatggggg tggccgttgt gggcggtgtg gtccgcctaa 240aaccatgttc ctcaaagatc atttgttgcc caacactggg ttgctgacca gaagtgccag 300gaagctgaat accatttcca gtgtcatacc cagggtgggt gacgaaaggg gtcttttgaa 360ctgtggaagg aacatccaag atctctggtc catgaagatt ggggtgtgga agggttacca 420gttggggaag ctcgtctgtc tttttccttc caatcagggg ctcgctcttc tgattattct 480tcagggcaat gacataaatt gtatattcgg tcccggttcc aggccagtaa tagtagcctc 540tgtgacacca gggcggggcc gagggaccct tctnttggaa gagaccagct tctcatactt 600gatgatgagn ccggtaatcc tggcacgtgg nggttgcatg atnccaccaa ggaaatnggn 660gggggnggac ctgcccggcg gccgttcnaa agcccaattc cacacacttg gnggccgtac 720tatggatccc actcngtcca acttggngga atatggcata actttt 766 226 364 DNA Homo sapiens 226tcgagcggcc gcccgggcag gtccttgacc ttttcagcaa gtgggaaggt gtaatccgtc 60tccacagaca aggccaggac tcgtttgtac ccgttgatga tagaatgggg tactgatgca 120acagttgggt agccaatctg cagacagaca ctggcaacat tgcggacacc ctccaggaag 180cgagaatgca gagtttcctc tgtgatatca agcacttcag ggttgtagat gctgccattg 240tcgaacacct gctggatgac cagcccaaag gagaaggggg agatgttgag catgttcagc 300agcgtggctt cgctggctcc cactttgtct ccagtcttga tcagacctcg gccgcgacca 360cgct 364 227 275 DNA Homo sapiens 227agcgtggtcg cggccgaggt ctgtcctaca gtcctcagga ctctactccc tcagcagcgt 60ggtgaccgtg ccctccagca acttcggcac ccagacctac acctgcaacg tagatcacaa 120gcccagcaac accaaggtgg acaagagagt tgagcccaaa tcttgtgaca aaactcacac 180atgcccaccg tgcccagcac ctgaactcct ggggggaccg tcagtcttcc tcttcccccg 240catccccctt ccaaacctgc ccgggcggcc gctcg 275 228 275 DNA Homo sapiens 228cgagcggccg cccgggcagg tttggaaggg ggatgcgggg gaagaggaag actgacggtc 60cccccaggag ttcaggtgct gggcacggtg ggcatgtgtg agttttgtca caagatttgg 120gctcaactct cttgtccacc ttggtgttgc tgggcttgtg atctacgttg caggtgtagg 180tctgggtgcc gaagttgctg gagggcacgg tcaccacgct gctgagggag tagagtcctg 240aggactgtag gacagacctc ggccgcgacc acgct 275 229 40 DNA Homo sapiens modified_base (1) Where n is a, c, g or t 229nggnnggtcc ggncngncag gaccactcnt cttcgaaata 40 230 208 DNA Homo sapiens 230agcgtggtcg cggccgaggt cctcacttgc ctcctgcaaa gcaccgatag ctgcgctctg 60gaagcgcaga tctgttttaa agtcctgagc aatttctcgc accagacgct ggaagggaag 120tttgcgaatc agaagttcag tggacttctg ataacgtcta atttcacgga gcgccacagt 180accaggacct gcccgggcgg ccgctcga 208 231 208 DNA Homo sapiens modified_base (33) Where n is a, c, g or t 231tcgagcggcc gcccgggcag gtcctggtac tgnggcgctc cgtgaaatta gacgttatca 60gaagtccact gaacttctga ttcgcaaact tcccttccag cgtctggtgc gagaaattgc 120tcaggacttt aaaacagatc tgcgcttcca gagcgcagct atcggtgctt tgcaggaggc 180aagtgaggac ctcggccgcg accacgct 208 232 332 DNA Homo sapiens 232tcgagcggcc gcccgggcag gtccacatcg gcagggtcgg agccctggcc gccatactcg 60aactggaatc catcggtcat gctctcgccg aaccagacat gcctcttgtc cttggggttc 120ttgctgatgt accagttctt ctgggccaca ctgggctgag tggggtacac gcaggtctca 180ccagtctcca tgttgcagaa gactttgatg gcatccaggt tgcagccttg gttggggtca 240atccagtact ctccactctt ccagtcagag tggcacatct tgaggtcacg gcaggtgcgg 300gcggggttct tgacctcggc cgcgaccacg ct 332 233 415 DNA Homo sapiens modified_base (6) Where n is a, c, g or t 233gtgggnttga acccntttna nctccgcttg gtaccgagct cggatccact agtaacggcc 60gccagtgtgc tggaattcgg cttagcgtgg tcgcggccga ggtcaagaac cccgcccgca 120cctgccgtga cctcaagatg tgccactctg actggaagag tggagagtac tggattgacc 180ccaaccaagg ctgcaacctg gatgccatca aagtcttctg caacatggag actggtgaga 240cctgcgtgta ccccactcag cccagtgtgg cccagaagaa ctggtacatc agcaagaacc 300ccaaggacaa gaggcatgtc tggttcggcg agagcatgac cgatggattc cagttcgagt 360atggcggcca gggctccgac cctgccgatg tggacctgcc cgggcggccg ctcga 415 234 776 DNA Homo sapiens modified_base (505) Where n is a, c, g or t 234agcgtggtcg cggccgaggt ctgggatgct cctgctgtca cagtgagata ttacaggatc 60acttacggag aaacaggagg aaatagccct gtccaggagt tcactgtgcc tgggagcaag 120tctacagcta ccatcagcgg ccttaaacct ggagttgatt ataccatcac tgtgtatgct 180gtcactggcc gtggagacag ccccgcaagc agcaagccaa tttccattaa ttaccgaaca 240gaaattgaca aaccatccca gatgcaagtg accgatgttc aggacaacag cattagtgtc 300aagtggctgc cttcaagttc ccctgttact ggttacagag taaccaccac tcccaaaaat 360ggaccaggac caacaaaaac taaaactgca ggtccagatc aaacagaaat gactattgaa 420ggcttgcagc ccacagtgga gtatgtggtt aagtgtctat gctcagaatc caagcggaga 480gaagtcagcc tctggttcag actgnaagta accaacattg atcgcctaaa ggactggcat 540tcactgatgn ggatgccgat tccatcaaaa ttgnttggga aaacccacag gggcaagttt 600ncangtcnag gnggacctac tcgagccctg aggatggaat ccttgactnt tccttnncct 660gatggggaaa aaaaaccttn aaaacttgaa ggacctgccc gggcggccgt ncaaaaccca 720attccacccc cttgggggcg ttctatgggn cccactcgga ccaaacttgg ggtaan 776 235 805 DNA Homo sapiens modified_base (637) Where n is a, c, g or t 235tcgagcggcc gcccgggcag gtccttgcag ctctgcagtg tcttcttcac catcaggtgc 60agggaatagc tcatggattc catcctcagg gctcgagtag gtcaccctgt acctggaaac 120ttgcccctgt gggctttccc aagcaatttt gatggaatcg gcatccacat cagtgaatgc 180cagtccttta gggcgatcaa tgttggttac tgcagtctga accagaggct gactctctcc 240gcttggattc tgagcataga cactaaccac atactccact gtgggctgca agccttcaat 300agtcatttct gtttgatctg gacctgcagt tttagttttt gttggtcctg gtccattttt 360gggagtggtg gttactctgt aaccagtaac aggggaactt gaaggcagcc acttgacact 420aatgctgttg tcctgaacat cggtcacttg catctgggat ggtttgtcaa tttctgttcg 480gtaattaatg gaaattggct tgctgcttgc ggggcttgtc tccacggcca gtgacagcat 540acacagtgat ggtataatca actccaggtt taagccgctg atggtagctg aaactttgct 600ccaggcacaa gtgaactcct gacagggcta tttcctnctg ttctccgtaa gtgatcctgt 660aatatctcac tgggacagca ggangcattc caaaacttcg ggcgngaccc cctaagccga 720attntgcaat atncatcaca ctggcgggcg ctcgancatt cattaaaagg cccaatcncc 780cctataggga gtntantaca attng 805 236 262 DNA Homo sapiens 236tcgagcggcc gcccgggcag gtcacttttg gtttttggtc atgttcggtt ggtcaaagat 60aaaaactaag tttgagagat gaatgcaaag gaaaaaaata ttttccaaag tccatgtgaa 120attgtctccc atttttttgg cttttgaggg ggttcagttt gggttgcttg tctgtttccg 180ggttgggggg aaagttggtt gggtgggagg gagccaggtt gggatggagg gagtttacag 240gaagcagaca gggccaacgt cg 262 237 372 DNA Homo sapiens 237agcgtggtcg cggccgaggt cctcaccaga ggtgccacct acaacatcat agtggaggca 60ctgaaagacc agcagaggca taaggttcgg gaagaggttg ttaccgtggg caactctgtc 120aacgaaggct tgaaccaacc tacggatgac tcgtgctttg acccctacac agtttcccat 180tatgccgttg gagatgagtg ggaacgaatg tctgaatcag gctttaaact gttgtgccag 240tgcttaggct ttggaagtgg tcatttcaga tgtgattcat ctagatggtg ccatgacaat 300ggtgtgaact acaagattgg agagaagtgg gaccgtcagg gagaaaatgg acctgcccgg 360gcggccgctc ga 372 238 372 DNA Homo sapiens 238tcgagcggcc gcccgggcag gtccattttc tccctgacgg tcccacttct ctccaatctt 60gtagttcaca ccattgtcat ggcaccatct agatgaatca catctgaaat gaccacttcc 120aaagcctaag cactggcaca acagtttaaa gcctgattca gacattcgtt cccactcatc 180tccaacggca taatgggaaa ctgtgtaggg gtcaaagcac gagtcatccg taggttggtt 240caagccttcg ttgacagagt tgcccacggt aacaacctct tcccgaacct tatgcctctg 300ctggtctttc agtgcctcca ctatgatgtt gtaggtggca cctctggtga ggacctcggc 360cgcgaccacg ct 372 239 720 DNA Homo sapiens modified_base (478) Where n is a, c, g or t 239tcgagcggcc gcccgggcag gtccaccata agtcctgata caaccacgga tgagctgtca 60ggagcaaggt tgatttcttt cattggtccg gtcttctcct tgggggtcac ccgcactcga 120tatccagtga gctgaacatt gggtggtgtc cactgggcgc tcaggcttgt gggtgtgacc 180tgagtgaact tcaggtcagt tggtgcagga atagtggtta ctgcagtctg aaccagaggc 240tgactctctc cgcttggatt ctgagcatag acactaacca catactccac tgtgggctgc 300aagccttcaa tagtcatttc tgtttgatct ggacctgcag ttttagtttt tgttggtcct 360ggtccatttt tgggagtggt ggttactctg taaccagtaa caggggaact tgaaggcagc 420cacttgacac taatgctgtt gtcctgaaca tcggtcactt gcatctggga tggtttgnca 480atttctgttc ggtaattaat ggaaattggc ttgctgcttg cggggctgtc tccacggcca 540gtgacagcat acacagngat ggnatnatca actccaagtt taaggccctg atggtaactt 600taaacttgct cccagccagn gaacttccgg acagggtatt tcttctggtt ttccgaaagn 660gancctggaa tnntctcctt ggancagaag gancntccaa aacttgggcc ggaacccctt 720 240 691 DNA Homo sapiens modified_base (564) Where n is a, c, g or t 240agcgtggtcg cggccgaggt cctgtcagag tggcactggt agaagttcca ggaaccctga 60actgtaaggg ttcttcatca gtgccaacag gatgacatga aatgatgtac tcagaagtgt 120cctggaatgg ggcccatgag atggttgtct gagagagagc ttcttgtcct acattcggcg 180ggtatggtct tggcctatgc cttatggggg tggccgttgt gggcggtgtg gtccgcctaa 240aaccatgttc ctcaaagatc atttgttgcc caacactggg ttgctgacca gaagtgccag 300gaagctgaat accatttcca gtgtcatacc cagggtgggt gacgaaaggg gtcttttgaa 360ctgtggaagg aacatccaag atctctggtc catgaagatt ggggtgtgga agggttacca 420gttggggaag ctcgtctgtc tttttccttc caatcagggg ctcgctcttc tgattattct 480tcagggcaat gacataaatt gtatattcgg ttcccggttc caggccagta atagtagcct 540cttgtgacac caggcggggc ccanggacca cttctctggg angagaccca gcttctcata 600cttgatgatg taacccggta atcctgcacg tggcggctgn catgatacca ncaaggaatt 660gggtgnggng gacctgcccg gcggccctcn a 691 241 808 DNA Homo sapiens modified_base (680) Where n is a, c, g or t 241agcgtggtcg cggccgaggt ctgggatgct cctgctgtca cagtgagata ttacaggatc 60acttacggag aaacaggagg aaatagccct gtccaggagt tcactgtgcc tgggagcaag 120tctacagcta ccatcagcgg ccttaaacct ggagttgatt ataccatcac tgtgtatgct 180gtcactggcc gtggagacag ccccgcaagc agcaagccaa tttccattaa ttaccgaaca 240gaaattgaca aaccatccca gatgcaagtg accgatgttc aggacaacag cattagtgtc 300aagtggctgc cttcaagttc ccctgttact ggttacagag taaccaccac tcccaaaaat 360ggaccaggac caacaaaaac taaaactgca ggtccagatc aaacagaaat gactattgaa 420ggcttgcagc ccacagtgga gtatgtggtt agtgtctatg ctcagaatcc aagcggagag 480agtcagcctc tggttcagac tgcagtaacc actattcctg caccaactga cctgaagttc 540actcaggtca cacccacaag cctgagccgc cagtggacac cacccaatgt tcactcactg 600gatatcgagt gcgggtgacc cccaaggaga agacccggac ccatgaaaga aatcaacctt 660gctcctgaca gctcatccgn gggtgtatca ggacttatgg gggactgccc cggcnggccg 720ntcgaaancg aattntgaaa tttccttcnc actgggnggc gnttcgagct tncttntana 780nggcccaatt cncctntagn gggtcgtn 808 242 26 DNA Homo sapiens modified_base (22) Where n is a, c, g or t 242agcgtggtcg cggccgaggt cnagga 26 243 697 DNA Homo sapiens modified_base (496) Where n is a, c, g or t 243tcgagcggcc gcccgggcag gtccaccaca cccaattcct tgctggtatc atggcagccg 60ccacgtgcca ggattaccgg ctacatcatc aagtatgaga agcctgggtc tcctcccaga 120gaagtggtcc ctcggccccg ccctggtgtc acagaggcta ctattactgg cctggaaccg 180ggaaccgaat atacaattta tgtcattgcc ctgaagaata atcagaagag cgagcccctg 240attggaagga aaaagacaga cgagcttccc caactggtaa cccttccaca ccccaatctt 300catggaccag agatcttgga tgttccttcc acagttcaaa agaccccttt cgtcacccac 360cctgggtatg acactggaaa tggtattcag cttcctggca cttctggtca gcaacccagt 420gttgggcaac aaatgatctt tgaggaacat ggttttaggc ggaccacacc gcccacaacg 480ggcaccccca taaggnatag gccaagacca taccccgccg aatgtaggac aagaagctct 540ntctcaacaa ccatctcatg ggccccattc caggacactt ctgagtacat catttcatgt 600catcctggtg ggcacttgat gaanaaccct tacagttcag ggttcctgga acttctacca 660gngccacttc tgacagganc ttgggcgnga ccaccct 697 244 373 DNA Homo sapiens 244agcgtggtcg cggccgaggt ccattttctc cctgacggtc ccacttctct ccaatcttgt 60agttcacacc attgtcatgg caccatctag atgaatcaca tctgaaatga ccacttccaa 120agcctaagca ctggcacaac agtttaaagc ctgattcaga cattcgttcc cactcatctc 180caacggcata atgggaaact gtgtaggggt caaagcacga gtcatccgta ggttggttca 240agccttcgtt gacagagttg cccacggtaa caacctcttc ccgaacctta tgcctctgct 300ggtctttcag tgcctccact atgatgttgt aggtggcacc tctggtgagg acctgcccgg 360gcggcccgct cga 373 245 307 DNA Homo sapiens 245agcgtggtcg cggccgaggt gtgccccaga ccaggaattc ggcttcgacg ttggccctgt 60ctgcttcctg taaactccct ccatcccaac ctggctccct cccacccaac caactttccc 120cccaacccgg aaacagacaa gcaacccaaa ctgaaccccc tcaaaagcca aaaaaatggg 180agacaatttc acatggactt tggaaaatat ttttttcctt tgcattcatc tctcaaactt 240agtttttatc tttgaccaac cgaacatgac caaaaaccaa aagtgacctg cccgggcggc 300cgctcga 307 246 372 DNA Homo sapiens 246tcgagcggcc gcccgggcag gtcctcacca gaggtgccac ctacaacatc atagtggagg 60cactgaaaga ccagcagagg cataaggttc gggaagaggt tgttaccgtg ggcaactctg 120tcaacgaagg cttgaaccaa cctacggatg actcgtgctt tgacccctac acagtttccc 180attatgccgt tggagatgag tgggaacgaa tgtctgaatc aggctttaaa ctgttgtgcc 240agtgcttagg ctttggaagt ggtcatttca gatgtgattc atctagatgg tgccatgaca 300atggtgtgaa ctacaagatt ggagagaagt gggaccgtca gggagaaaat ggacctcggc 360cgcgaccacg ct 372 247 348 DNA Homo sapiens modified_base (284) Where n is a, c, g or t 247tcgagcggcc gcccgggcag gtaccggggt ggtcagcgag gagccattca cactgaactt 60caccatcaac aacctgcggt atgaggagaa catgcagcac cctggctcca ggaagttcaa 120caccacggag agggtccttc agggcctgct caggtccctg ttcaagagca ccagtgttgg 180ccctctgtac tctggctgca gactgacttt gctcagacct gagaaacatg gggcagccac 240tggagtggac gccatctgca ccctccgcct tgatcccact ggtnctggac tggacanana 300gcggctatac ttgggagctg anccnaacct ttggcggnga cnccnctt 348 248 304 DNA Homo sapiens modified_base (125) Where n is a, c, g or t 248gaggactggc tcagctccca gtatagccgc tctctgtcca gtccaggacc agtgggatca 60aggcggaggg tgcagatggc gtccactcca gtggctgccc catgtttctc aagtctgagc 120aaagncagtc tgcagccaga gtacagaggg ccaacactgg tgctcttgaa cagggacctg 180agcaggccct gaaggaccct ctccgtggtg ttgaacttcc tggagccagg gtgctgcatg 240ttctcctcat accgcaggtt gttgatggtg aagttcagtg tgaatggctc ctcgctgacc 300accc 304 249 400 DNA Homo sapiens modified_base (308) Where n is a, c, g or t 249agcgtggtcg cggccgaggt ccaccacacc caattccttg ctggtatcat ggcagccgcc 60acgtgccagg attaccggct acatcatcaa gtatgagaag cctgggtctc ctcccagaga 120agtggtccct cggccccgcc ctggtgtcac agaggctact attactggcc tggaaccggg 180aaccgaatat acaatttatg tcattgccct gaagaataat cagaagagcg agcccctgat 240tggaaggaaa aagacagacg agcttcccca actggtaacc cttccacacc ccaatcttca 300tggaccanan ancttggatn gtcctttcac nggttnaaaa aacccttttc gcccccccac 360cttggggatt aaccttggga aanggggatt tnaccnttcc 400 250 400 DNA Homo sapiens modified_base (338) Where n is a, c, g or t 250tcgagcggcc gcccgggcag gtcctgtcag agtggcactg gtagaagttc caggaaccct 60gaactgtaag ggttcttcat cagtgccaac aggatgacat gaaatgatgt actcagaagt 120gtcctggaat ggggcccatg agatggttgt ctgagagaga gcttcttgtc ctacattcgg 180cgggtatggt cttggcctat gccttatggg ggtggccgtt gtgggcggtg tggtccgcct 240aaaaccatgt tcctcaaaga tcatttgttg cccaacactg ggttgctgac cagaagtgcc 300aggaagctga ataccatttc cagtgtcata cccagggngg gtgaccaaag ggggtcnttt 360ngacctggng aaaggaacca tccaaaanct ctgncccatg 400 251 514 DNA Homo sapiens modified_base (8) Where n is a, c, g or t 251agcgtggncg cggccgaggt ctgaggatgt aaactcttcc caggggaagg ctgaagtgct 60gaccatggtg ctactgggtc cttctgagtc agatatgtga ctgatgngaa ctgaagtagg 120tactgtagat ggtgaagtct gggtgtccct aaatgctgca tctccagagc cttccatcat 180taccgtttct tcttttgcta tgggatgaga cactgttgag tattctctaa agtcaccact 240gaaatcttcc tccaaaggaa aacctgtgga aaagcccctt atttctgccc cataatttgg 300ttctcctaat cnctctgaaa tcactatttc cctggaangt ttgggaaaaa nngggcnacc 360tgncantgga aantggatan aaagatccca ccattttacc caacnagcag aaagtgggaa 420nggtaccgaa aagctccaag taanaaaaag gagggaagta aaggtcaagt gggcaccagt 480ttcaaacaaa actttcccca aactatanaa ccca 514 252 501 DNA Homo sapiens modified_base (20) Where n is a, c, g or t 252aagcggccgc ccgggcaggn ncagnagtgc cttcgggact gggntcaccc ccaggtctgc 60ggcagttgtc acagcgccag ccccgctggc ctccaaagca tgtgcaggag caaatggcac 120cgagatattc cttctgccac tgttctccta cgtggtatgt cttcccatca tcgtaacacg 180ttgcctcatg agggtcacac ttgaattctc cttttccgtt cccaagacat gtgcagctca 240tttggctggc tctatagttt ggggaaagtt tgttgaaact gtgccactga cctttacttc 300ctccttctct actggagctt tccgtacctt ccacttctgc tgntggnaaa aagggnggaa 360cntcttatca atttcattgg acagtanccc nctttctncc caaaacatnc aagggaaaat 420attgattncn agagcggatt aaggaacaac ccnaattatg ggggccagaa ataaaggggg 480cttttccaca ggtnttttcc t 501 253 226 DNA Homo sapiens 253tcgagcggcc gcccgggcag gtctgcaggc tattgtaagt gttctgagca catatgagat 60aacctgggcc aagctatgat gttcgatacg ttaggtgtat taaatgcact tttgactgcc 120atctcagtgg atgacagcct tctcactgac agcagagatc ttcctcactg tgccagtggg 180caggagaaag agcatgctgc gactggacct cggccgcgac cacgct 226 254 226 DNA Homo sapiens 254agcgtggtcg cggccgaggt ccagtcgcag catgctcttt ctcctgccca ctggcacagt 60gaggaagatc tctgctgtca gtgagaaggc tgtcatccac tgagatggca gtcaaaagtg 120catttaatac acctaacgta tcgaacatca tagcttggcc caggttatct catatgtgct 180cagaacactt acaatagcct gcagacctgc ccgggcggcc gctcga 226 255 427 DNA Homo sapiens modified_base (327) Where n is a, c, g or t 255cgagcggccg cccgggcagg tccagactcc aatccagaga accaccaagc cagatgtcag 60aagctacacc atcacaggtt tacaaccagg cactgactac aagatctacc tgtacacctt 120gaatgacaat gctcggagct cccctgtggt catcgacgcc tccactgcca ttgatgcacc 180atccaacctg cgtttcctgg ccaccacacc caattccttg ctggtatcat ggcagccgcc 240acgtgccagg attaccggct acatcatcaa gtatgagaag cctgggtctc ctcccagaga 300agtggtccct cggccccgcc ctggtgncac agaagctact attactggcc tggaaccggg 360aaccgaatat acaatttatg tcattgccct gaagaataat canaagagcg agcccctgat 420tggaagg 427 256 535 DNA Homo sapiens modified_base (347) Where n is a, c, g or t 256agcgtggtcg cggccgaggt cctgtcagag tggcactggt agaagttcca ggaaccctga 60actgtaaggg ttcttcatca gtgccaacag gatgacatga aatgatgtac tcagaagtgt 120cctggaatgg ggcccatgag atggttgtct gagagagagc ttcttgtcct gtctttttcc 180ttccaatcag gggctcgctc ttctgattat tcttcagggc aatgacataa attgtatatt 240cggttcccgg ttccaggcca gtaatagtag cctctgtgac accagggcgg ggccgaggga 300ccacttctct gggaggagac ccaggcttct catacttgat gatgtanccg gtaatcctgg 360caccgtggcg gctgccatga taccagcaag gaattgggtg tggtggccaa gaaacgcagg 420ttggatggtg catcaatggc agtggaggcg tcgatnacca caggggagct ccgancattg 480tcattcaagg tggacaggta gaatcttgta atcaggtgcc tggtttgtaa acctg 535 257 544 DNA Homo sapiens modified_base (495) Where n is a, c, g or t 257tcgagcggcc gcccgggcag gtttcgtgac cgtgacctcg aggtggacac caccctcaag 60agcctgagcc agcagatcga gaacatccgg agcccagagg gcagccgcaa gaaccccgcc 120cgcacctgcc gtgacctcaa gatgtgccac tctgactgga agagtggaga gtactggatt 180gaccccaacc aaggctgcaa cctggatgcc atcaaagtct tctgcaacat ggagactggt 240gagacctgcg tgtaccccac tcagcccagt gtggcccaga agaactggta catcagcaag 300aaccccaagg acaagaagca tgtctggttc ggcgaaagca tgaccgatgg attccagttc 360gagtatggcg gccagggctc cgaccctgcc gatgtggacc tcggccgcga ccacgctaag 420cccgaattcc agcacactgg cggccgttac tagtgggatc cgagcttcgg taccaagctt 480ggcgtaatca tgggncatag ctgtttcctg ngtgaaaatg gtattccgct tcacaatttc 540ccac 544 258 418 DNA Homo sapiens 258agcgtggtcg cggccgaggt ccacatcggc agggtcggag ccctggccgc catactcgaa 60ctggaatcca tcggtcatgc tctcgccgaa ccagacatgc ctcttgtcct tggggttctt 120gctgatgtac cagttcttct gggccacact gggctgagtg gggtacacgc aggtctcacc 180agtctccatg ttgcagaaga ctttgatggc atccaggttg cagccttggt tggggtcaat 240ccagtactct ccactcttcc agtcagagtg gcacatcttg aggtcacggc aggtgcgggc 300ggggttcttg cggctgccct ctgggctccg gatgttctcg atctgctggc tcaagctctt 360gaagggtggt gtccacctcg aggtcacggt cacgaaacct gcccgggcgg ccgctcga 418 259 377 DNA Homo sapiens modified_base (320) Where n is a, c, g or t 259agcgtggtcg cggccgaggt caagaacccc gcccgcacct gccgtgacct caagatgtgc 60cactctgact ggaagagtgg agagtactgg attgacccca accaaggctg caacctggat 120gccatcaaag tcttctgcaa catggagact ggtgagacct gcgtgtaccc cactcagccc 180agtgtggccc agaagaactg gtacatcagc aagaacccca aggacaagag gcatgtctgg 240ttcggcgaga gcatgaccga tggattccag ttcgagtatg gcggccaggg ctccgaccct 300gccgatgtgg acctgcccgn gccggnccgc tcgaaaagcc cnaatttcca gncacacttg 360gccggccgtt actactg 377 260 332 DNA Homo sapiens 260tcgagcggcc gcccgggcag gtccacatcg gcagggtcgg agccctggcc gccatactcg 60aactggaatc catcggtcat gctctcgccg aaccagacat gcctcttgtc cttggggttc 120ttgctgatgt accagttctt ctgggccaca ctgggctgag tggggtacac gcaggtctca 180ccagtctcca tgttgcagaa gactttgatg gcatccaggt tgcagccttg gttggggtca 240atccagtact ctccactctt ccagtcagag tggcacatct tgaggtcacg gcaggtgcgg 300gcggggttct tgacctcggc cgcgaccacg ct 332 261 94 DNA Homo sapiens 261cgagcggccg cccgggcagg tcccccccct tttttttttt tttttttttt tttttttttt 60tttttttttt tttttttttt tttttttttt tttt 94 262 650 DNA Homo sapiens modified_base (412) Where n is a, c, g or t 262agcgtggtcg cggccgaggt ctggcattcc ttcgacttct ctccagccga gcttcccaga 60acatcacata tcactgcaaa aatagcattg catacatgga tcaggccagt ggaaatgtaa 120agaaggccct gaagctgatg gggtcaaatg aaggtgaatt caaggctgaa ggaaatagca 180aattcaccta cacagttctg gaggatggtt gcacgaaaca cactggggaa tggagcaaaa 240cagtctttga atatcgaaca cgcaaggctg tgagactacc tattgtagat attgcaccct 300atgacattgg tggtcctgat caagaatttg gtgtggacgt tggccctgtt tgctttttat 360aaaccaaact ctatctgaaa tcccaacaaa aaaaatttaa ctccatatgt gntcctcttg 420ttctaatctt ggcaaccagt gcaagtgacc gacaaaattc cagttattta tttccaaaat 480gtttggaaac agtataattt gacaaagaaa aaaggatact tctctttttt tggctggtcc 540accaaataca attcaaaagg ctttttggtt ttattttttt anccaattcc aatttcaaaa 600tgtctcaatg gngcttataa taaaataaac tttcaccctt nttttntgat 650 263 573 DNA Homo sapiens modified_base (453) Where n is a, c, g or t 263agcgtggtcg cggccgaggt ctgggatgct cctgctgtca cagtgagata ttacaggatc 60acttacggag aaacaggagg aaatagccct gtccaggagt tcactgtgcc tgggagcaag 120tctacagcta ccatcagcgg ccttaaacct ggagttgatt ataccatcac tgtgtatgct 180gtcactggcc gtggagacag ccccgcaagc agcaagccaa tttccattaa ttaccgaaca 240gaaattgaca aaccatccca gatgcaagtg accgatgttc aggacaacag cattagtgtc 300aagtggctgc cttcaagttc ccctgttact ggttacagaa gtaaccacca ctcccaaaaa 360tggaccagga ccaacaaaaa ctaaaactgc aggtccagat caaacagaaa atggactatt 420gaaggcttgc agcccacagt ggaagtatgt ggntaggngt ctatgctcag aatcccaagc 480cggagaaagt cagccttctg gtttagactg cagtaaccaa cattgatcgc cctaaaggac 540tggncattca cttggatggt ggatgtccaa ttc 573 264 550 DNA Homo sapiens modified_base (39) Where n is a, c, g or t 264tcgagcggcc gcccgggcag gtccttgcag ctctgcagng tcttcttcac catcaggtgc 60agggaatagc tcatggattc catcctcagg gctcgagtag gtcaccctgt acctggaaac 120ttgcccctgt gggctttccc aagcaatttt gatggaatcg acatccacat cagngaatgc 180cagtccttta gggcgatcaa tgttggttac tgcagtctga accagaggct gactctctcc 240gcttggattc tgagcataga cactaaccac atactccact gtgggctgca agccttcaat 300agtcatttct gtttgatctg gacctgcagt tttaagtttt tggtggtcct gncccatttt 360tgggaagtgg ggggttactc tgtaaccagt aacaggggaa cttgaaggca gccacttgac 420actaatgctg ttgtcctgaa catcggtcac ttgcatctgg ggatggtttt gacaatttct 480ggttcggcaa attaatggaa attggcttgc tgcttggcgg ggctgnctcc acgggccagt 540gacagcatac 550 265 596 DNA Homo sapiens modified_base (347) Where n is a, c, g or t 265tcgagcggcc gcccgggcag gtccttgcag ctctgcagtg tcttcttcac catcaggtgc 60agggaatagc tcatggattc catcctcagg gctcgagtag gtcaccctgt acctggaaac 120ttgcccctgt gggctttccc aagcaatttt gatggaatcg acatccacat cagtgaatgc 180cagtccttta gggcgatcaa tgttggttac tgcagtctga accagaggct gactctctcc 240gcttggattc tgagcataga cactaaccac atactccact gtgggctgca agccttcaat 300agtcatttct gtttgatctg gacctgcagt tttaagtttt tgttggncct gnnccatttt 360tggggaaggg gtggttactc ttgtaaccag taacagggga acttgaagca gccacttgac 420actaatgctg gtggcctgaa catcggtcac ttgcatctgg gatggtttgg tcaatttctg 480ttcggtaatt aatgggaaat tggcttactg gcttgcgggg gctgtctcca cggncagtga 540caagcataca caggngatgg gtataatcaa ctccaggttt aaggccnctg atggta 596 266 506 DNA Homo sapiens modified_base (393) Where n is a, c, g or t 266agcgtggtcg cggccgaggt ctgggatgct cctgctgtca cagtgagata ttacaggatc 60acttacggag aaacaggagg aaatagccct gtccaggagt tcactgtgcc tgggagcaag 120tctacagcta ccatcagcgg ccttaaacct ggagttgatt ataccatcac tgtgtatgct 180gtcactggcc gtggagacag ccccgcaagc agtaagccaa tttccattaa ttaccgaaca 240gaaattgaca aaccatccca gatgcaagtg accgatgttc aggacaacag cattagtgtc 300aagtggctgc cttcaagttc ccctgttact ggttacagag taaccaccac tcccaaaaat 360gggaccagga ccaacaaaaa actaaaactg canggtccag atcaaacaga aatgactatt 420gaaggcttgc agcccacagt ggagtatgtg ggttagtgtc tatgctcaga atnccaagcg 480gagagagtca gcctctggtt cagact 506 267 548 DNA Homo sapiens modified_base (346) Where n is a, c, g or t 267tcgagcggcc gcccgggcag gtcagcgctc tcaggacgtc accaccatgg cctgggctct 60gctcctcctc accctcctca ctcagggcac agggtcctgg gcccagtctg ccctgactca 120gcctccctcc gcgtccgggt ctcctggaca gtcagtcacc atctcctgca ctggaaccag 180cagtgacgtt ggtgcttatg aatttgtctc ctggtaccaa caacacccag gcaaggcccc 240caaactcatg atttctgagg tcactaagcg gccctcaggg gtccctgatc gcttctctgg 300ctccaagtct ggcaacacgg cctccctgac cgtctctggg ctccangctg aggatgangc 360tgattattac tggaagctca tatgcaggca acaacaattg ggtgttcggc ggaagggacc 420aagctgaccg tnctaaggtc aagcccaagg cttgcccccc tcggtcactc tgttcccacc 480ctcctctgaa gaagctttca agccaacaan gncacactgg gtgtgtctca taagtggact 540ttctaccc 548 268 584 DNA Homo sapiens modified_base (98) Where n is a, c, g or t 268agcgtggtcg cggccgaggt ctgtagcttc tgtgggactt ccactgctca ggcgtcaggc 60tcaggtagct gctggccgcg tacttgttgt tgctttgntt ggagggtgtg gtggtctcca 120ctcccgcctt gacggggctg ctatctgcct tccaggccac tgtcacggct cccgggtaga 180agtcacttat gagacacacc agtgtggcct tgttggcttg aagctcctca gaggagggtg 240ggaacagagt gaccgagggg gcagccttgg gctgacctag gacggtcagc ttggtccctc 300cgccgaacac ccaattgttg ttgcctgcat atgagctgca gtaataatca gcctcatcct 360cagcctggag cccagagacn gtcaagggag gcccgtgttt gccaagactt ggaagccaga 420naagcgatca gggacccctg agggccgctt tacngacctc aaaaaatcat gaatttgggg 480ggcctttgcc tgggngttgg ttggtnacca gnaaaacaaa atttcataaa gcaccaacgt 540cactgctggt ttccagtgca ngaanatggt gaactgaant gtcc 584 269 368 DNA Homo sapiens modified_base (265) Where n is a, c, g or t 269agcgtggtcg cggccgaggt ccagcatcag gagccccgcc ttgccggctc tggtcatcgc 60ctttcttttt gtggcctgaa acgatgtcat caattcgcag tagcagaact gccgtctcca 120ctgctgtctt ataagtctgc agcttcacag ccaatggctc ccatatgccc agttccttca 180tgtccaccaa agtacccgtc tcaccattta caccccaggt ctcacagttc tcctgggtgt 240gcttggcccg aagggaggta agtanacgga tggtgctggt cccacagttc tggatcaggg 300tacgaggaat gacctctagg gcctgggcna caagccctgt atggacctgc ccgggcgggc 360ccgctcga 368 270 368 DNA Homo sapiens modified_base (54) Where n is a, c, g or t 270tcgagcggcc gcccgggcag gtccatacag ggctgttgcc caggccctag aggncattcc 60ttgtaccctg atccagaact gtgggaccag caccatccgt ctacttacct cccttcgggc 120caagcacacc caggagaact gtgagacctg gggtgtaaat ggngagacgg gtactttggt 180ggacatgaag gaactgggca tatgggagcc attggctgng aagctgcana cttataagac 240agcagtggag acggcagttc tgctactgcg aattgatgac atcgtttcag gccacaaaaa 300gaaaggcgat gaccanagcc ggcaaggcgg ggcttcctga tgctggacct cggccgccga 360ccacgctt 368 271 424 DNA Homo sapiens modified_base (279) Where n is a, c, g or t 271agcgtggtcg cggccgaggt ccactagagg tctgtgtgcc attgcccagg cagagtctct 60gcgttacaaa ctcctaggag ggcttgctgt gcggagggcc tgctatggtg tgctgcggtt 120catcatggag agtggggcca aaggctgcga ggttgtggtg tctgggaaac tccgaggaca 180gagggctaaa tccatgaagt ttgtggatgg cctgatgatc cacagcggag accctgttaa 240ctactacgtt gacactgctg tgcgccacgt gttgctcana cagggtgtgc tgggcatcaa 300ggtgaagatc atgctgccct gggacccanc tggcaaaaat ggcccttaaa aaccccttgc 360cntgaccacg tgaaccattt gtgngaaccc caagatgaan atacttgccc accacccccc 420attc 424 272 541 DNA Homo sapiens modified_base (422) Where n is a, c, g or t 272tcgagcggcc gcccgggcag gtctgccaag gagaccctgt tatgctgtgg ggactggctg 60gggcatggca ggcggctctg gcttcccacc cttctgttct gagatggggg tggtgggcag 120tatctcatct ttgggttcca caatgctcac gtggtcaggc aggggcttct tagggccaat 180cttaccagtt gggtcccagg gcagcatgat cttcaccttg atgcccagca caccctgtct 240gagcaacacg tggcgcacag cagtgtcaac gtagtagtta acagggtctc cgctgtggat 300catcaggcca tccacaaact tcatggattt agccctctgt cctcggagtt tcccaaaaca 360ccacaacctc gccagccttt gggccccact tcttcatgaa tgaaaccgca gcacaccatt 420ancaaggccc ttccgcacag gnaagccctt cctaaggagt tttgtaaacg caaaaaactc 480ttgcctgggg caaatgggca cacagacctn tantnggacc ttggnccgcg aaccaccgct 540t 541 273 579 DNA Homo sapiens modified_base (223) Where n is a, c, g or t 273agcgtggtcg cggccgaggt ctggccctcc tggcaaggct ggtgaagatg gtcaccctgg 60aaaacccgga cgacctggtg agagaggagt tgttggacca cagggtgctc gtggtttccc 120tggaactcct ggacttcctg gcttcaaagg cattagggga cacaatggtc tggatggatt 180gaagggacag cccggtgctc ctggtgtgaa gggtgaacct ggngcccctg gtgaaaatgg 240aactccaggt caaacaggag cccgngggct tcctggngag agaggacgtg ttggtgcccc 300tggcccanac ctgcccgggc ggccgctcna aaagccgaaa tccagnacac tggcggccgn 360tactantgga atccgaactt cggtaccaaa gcttggccgt aatcatggcc atagcttgtt 420ccctggggng gaaattggta ttccgctncc aattccacac aacataccga acccggaaag 480cattaaagtg taaaagccct gggggggcct aaatgangtg agcntaactc ncatttaatt 540ggcgttgcgc ttcactgccc cgcttttcca gtccgggna 579 274 330 DNA Homo sapiens modified_base (171) Where n is a, c, g or t 274tcgagcggcc gcccgggcag gtctgggcca ggggcaccaa cacgtcctct ctcaccagga 60agcccacggg ctcctgtttg acctggagtt ccattttcac caggggcacc aggttcaccc 120ttcacaccag gagcaccggg ctgtcccttc aatccatcca gaccattgtg ncccctaatg 180cctttgaagc caggaagtcc aggagttcca gggaaaccac gagcaccctg tggtccaaca 240actcctctct caccaggtcg tccgggtttt ccagggtgac catcttcacc agccttgcca 300ggagggccag acctcggccg cgaccacgct 330 275 97 DNA Homo sapiens modified_base (2) Where n is a, c, g or t 275ancgtggtcg cggccgaggt cctcaccaga ggtgncacct acaacatcat agtggaggca 60ctgaaagacc ancagaggca taaggttcgg gaagagg 97 276 610 DNA Homo sapiens modified_base (358) Where n is a, c, g or t 276tcgagcggcc gcccgggcag gtccattttc tccctgacgg tcccacttct ctccaatctt 60gtagttcaca ccattgtcat ggcaccatct agatgaatca catctgaaat gaccacttcc 120aaagcctaag cactggcaca acagtttaaa gcctgattca gacattcgtt cccactcatc 180tccaacggca taatgggaaa ctgtgtaggg gtcaaagcac gagtcatccg taggttggtt 240caagccttcg ttgacagagt tgtccacggt aacaacctct tcccgaacct tatgcctctg 300ctggtctttc agtgcctcca ctatgatgtt gtaggtggca cctctggtga ggacctcngn 360ccngaacaac gcttaagccc gnattctgca gaataatccc atcacacttg gcggccgctt 420cgancatgca tcntaaaagg ggccccaatt tcccccttat aagngaancc gtatttncca 480atttcactgg ncccgccgnt tttacaaacg ncggtgaact ggggaaaaac cctggcggtt 540acccaacttt aatcgccntt ggcagcacaa tccccccttt tcgnccancn tgggcgtaaa 600taaccgaaaa 610 277 38 DNA Homo sapiens modified_base (2) Where n is a, c, g or t 277ancgnggtcg cggccgangt nttttttctt nttttttt 38 278 443 DNA Homo sapiens modified_base (156) Where n is a, c, g or t 278agcgtggtcg cggccgaggt ctgaggttac atgcgtggtg gtggacgtga gccacgaaga 60ccctgaggtc aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa 120gccgcgggag gagcagtaca acagcacgta ccgggnggtc agcgtcctca ccgtcctgca 180ccagaattgg ttgaatggca aggagtacaa gngcaaggtt tccaacaaag ccntcccagc 240ccccntcgaa aaaaccattt ccaaagccaa agggcagccc cgagaaccac aggtgtacac 300cctgccccca tcccgggagg aaaagancaa naaccnggtt cagccttaac ttgcttggtc 360naangctttt tatcccaacg nacttccccc ntggaantgg gaaaaaccaa tgggccaanc 420cgaaaaacaa ttacaanaac ccc 443 279 348 DNA Homo sapiens modified_base (219) Where n is a, c, g or t 279tcgagcggcc gcccgggcag gtgtcggagt ccagcacggg aggcgtggtc ttgtagttgt 60tctccggctg cccattgctc tcccactcca cggcgatgtc gctgggatag aagcctttga 120ccaggcaggt caggctgacc tggttcttgg tcatctcctc ccgggatggg ggcagggtga 180acacctgggg ttctcggggc ttgccctttg gttttgaana tggttttctc gatgggggct 240ggaagggctt tgttgnaaac cttgcacttg actccttgcc attcacccag ncctggngca 300ggacggngag gacnctnacc acacggaacc gggctggtgg actgctcc 348 280 149 DNA Homo sapiens modified_base (18) Where n is a, c, g or t 280agcgtggtcg cggacgangt cctgtcagag tggnactggt agaagttcca ngaaccctga 60actgtaaggg ttcttcatca gtgccaacag gatgacatga aatgatgtac tcagaagngn 120cctggaatgg ggcccatgan atggttgcc 149 281 404 DNA Homo sapiens modified_base (383) Where n is a, c, g or t 281tcgagcggcc gcccgggcag gtccaccaca cccaattcct tgctggtatc atggcagccg 60ccacgtgcca ggattaccgg ctacatcatc aagtatgaga agcctgggtc tcctcccaga 120gaagtggtcc ctcggccccg ccctggtgtc acagaggcta ctattactgg cctggaaccg 180ggaaccgaat atacaattta tgtcattgcc ctgaagaata atcagaagag cgagcccctg 240attggaagga aaaagacaga cgagcttccc caactggtaa cccttccaca ccccaatctt 300catggaccag agatcttgga tgttccttcc acagttcaaa agaccccttt cggcaccccc 360cctgggtatg aacctgggaa aanggnantt aanctttcct ggca 404 282 507 DNA Homo sapiens modified_base (320) Where n is a, c, g or t 282agcgtggtcg cggccgaggt ctgggatgct cctgctgtca cagtgagata ttacaggatc 60acttacggag aaacaggagg aaatagccct gtccaggagt tcactgtgcc tgggagcaag 120tctacagcta ccatcagcgg ccttaaacct ggagttgatt ataccatcac tgtgtatgct 180gtcactggcc gtggagacag ccccgcaagc agcaagccaa tttccattaa ttaccgaaca 240gaaattgaca aaccatccca gatgcaagtg accgatgttc aggacaacag cattagtgtc 300aagtggctgc cttcaaggtn ccctggtact gggttacaga ntaaccacca ctcccaaaaa 360tggaccagga accacaaaaa cttaaactgc agggtccaga tcaaaacaga aatgactatt 420gaangcttgc agcccacagt gggagtatgn gggtagtgnc tatgcttcag aatccaagcg 480gaaaaangtc aagccttntg ggttcaa 507 283 325 DNA Homo sapiens modified_base (216) Where n is a, c, g or t 283tcgagcggcc gcccgggcag gtccttgcag ctctgcagtg tcttcttcac catcaggtgc 60agggaatagc tcatggattc catcctcagg gctcgagtag gtcaccctgt acctggaaac 120ttgcccctgt gggctttccc aagcaatttt gatggaatcg acatccacat cagtgaatgc 180cagtccttta gggcgatcaa tgttggttac tgcagnctga accagaggct gactctctcc 240gcttggattc tgagcataga cactaaccac atactccact gtgggctgca anccttcaat 300aanncatttc tgtttgatct ggacc 325 284 331 DNA Homo sapiens modified_base (54) Where n is a, c, g or t 284tcgagcggcc gcccgggcag gtctggtggg gtcctggcac acgcacatgg gggngttgnt 60ctnatccagc tgcccagccc ccattggcga gtttgagaag gtgtgcagca atgacaacaa 120naccttcgac tcttcctgcc acttctttgc cacaaagtgc accctggagg gcaccaagaa 180gggccacaag ctccacctgg actacatcgg gccttgcaaa tacatccccc cttgcctgga 240ctctgagctg accgaattcc cccttgcgca tgcgggactg gctcaagaac cgtcctggca 300cccttgtatg anagggatga agacacnacc c 331 285 509 DNA Homo sapiens modified_base (316) Where n is a, c, g or t 285agcgtggtcg cggccgaggt ctgtcctaca gtcctcagga ctctactccc tcagcagcgt 60ggtgaccgtg ccctccagca acttcggcac ccagacctac acctgcaacg tagatcacaa 120gcccagcaac accaaggtgg acaagagagt tgagcccaaa tcttgtgaca aaactcacac 180atgcccaccg tgcccagcac ctgaactcct ggggggaccg tcagtcttcc tcttcccccg 240catccccctt ccaaacctgc ccgggcggcc gctcgaaagc cgaattccag cacactggcg 300gccggtacta gtgganccna acttggnanc caacctggng gaantaatgg gcataanctg 360tttctggggg gaaattggta tccngtttac aattcccnca caacatacga gccggaagca 420taaaagngta aaagcctggg ggnggcctan tgaagtgaag ctaaactcac attaattngc 480gttgccgctc actggcccgc ttttccagc 509 286 336 DNA Homo sapiens modified_base (188) Where n is a, c, g or t 286tcgagcggcc gcccgggcag gtttggaagg gggatgcggg ggaagaggaa gactgacggt 60ccccccagga gttcaggtgc tgggcacggt gggcatgtgt gagttttgtc acaagatttg 120ggctcaactc tcttgtccac cttggtgttg ctgggcttgt gatctacgtt gcaggtgtag 180gtctgggngc cgaagttgct ggagggcacg gtcaccacgc tgctgaggga gtagagtcct 240gaggactgta ngacagacct cggccgngac cacgctaagc cgaattctgc agatatccat 300cacactggcg gccgctccga gcatgcattt tagagg 336 287 30 DNA Homo sapiens modified_base (8) Where n is a, c, g or t 287agcgtggncg cggacganga caacaacccc 30 288 316 DNA Homo sapiens modified_base (22) Where n is a, c, g or t 288tcgagcggcc gcccgggcag gnccacatcg gcagggtcgg agccctggcc gccatactcg 60aactggaatc catcggtcat gctcttgccg aaccagacat gcctcttgtc cttggggttc 120ttgctgatgn accagttctt ctgggccaca ctgggctgag tggggtacac gcaggtctca 180ccagtctcca tgttgcagaa gactttgatg gcatccaggt tgcagccttg gttggggtca 240atccagtact ctccactctt ccagtcagag tggcacatct tgaggtcacg gcaggtgcgg 300gcggggttct tgacct 316 289 308 DNA Homo sapiens modified_base (36) Where n is a, c, g or t 289agcgtggtcg cggccgaggt ccagcctgga gataanggtg aaggtggtgc ccccggactt 60ccaggtatag ctggacctcg tggtagccct ggtgagagag gtgaaactgg ccctccagga 120cctgctggtt tccctggtgc tcctggacag aatggtgaac ctggnggtaa aggagaaaga 180ggggctccgg ntganaaagg tgaaggaggc cctcctgnat tggcaggggc cccangactt 240agaggtggag ctggcccccc tggccccgaa ggaggaaagg gtgctgctgg tcctcctggg 300ccacctgg 308 290 324 DNA Homo sapiens modified_base (184) Where n is a, c, g or t 290tcgagcggcc gcccgggcag gtctgggcca ggaggaccaa taggaccagt aggacccctt 60gggccatctt tccctgggac accatcagca cctggaccgc ctggttcacc cttgtcaccc 120tttggaccag gacttccaag acctcctctt tctccaggca ttccttgcag accaggagta 180ccancagcac caggtggccc aggaggacca gcagcaccct ttcctccttc gggaccaggg 240ggaccagctc cacctctaag tcctggggcc cctgccaatc caggagggcc tccttcacct 300ttctcacccg gagcccctct ttct 324 291 278 DNA Homo sapiens modified_base (249) Where n is a, c, g or t 291tcgagcggcc gcccgggcag gtccaccggg atattcgggg gtctggcagg aatgggaggc 60atccagaacg agaaggagac catgcaaagc ctgaacgacc gcctggcctc ttacctggac 120agagtgagga gcctggagac cgacaaccgg aggctggaga gcaaaatccg ggagcacttg 180gagaagaagg gaccccaggt cagagactgg agccattact tcaagatcat cgaggacctg 240agggctcana tcttcgcaaa tactgcngac aatgcccg 278 292 299 DNA Homo sapiens modified_base (6) Where n is a, c, g or t 292atgcgnggtc gcggccgang accanctctg gctcatactt gactctaaag ncntcaccag 60nanttacggn cattgccaat ctgcagaacg atgcgggcat tgtccgcant atttgcgaag 120atctgagccc tcaggncctc gatgatcttg aagtaanggc tccagtctct gacctggggt 180cccttcttct ccaagtgctc ccggattttg ctctccagcc tccggttctc ggtctccaag 240ncttctcact ctgtccagga aaagaggcca ggcggncgat cagggctttt gcatggact 299 293 101 DNA Homo sapiens 293agcgtggtcg cggccgaggt tgtacaagct tttttttttt tttttttttt tttttttttt 60tttttttttt tttttttttt tttttttttt tttttttttt t 101 294 285 DNA Homo sapiens modified_base (64) Where n is a, c, g or t 294tcgagcggcc gcccgggcag gtctgccaac accaagattg gcccccgccg catccacaca 60gttngtgtgc ggggaggtaa caagaaatac cgtgccctga ggntggacgn ggggaatttc 120tcctggggct cagagtgttg tactcgtaaa acaaggatca tcgatgttgt ctacaatgca 180tctaataacg agctggttcg taccaagacc ctggtgaaga attgcatcgt gctcatngac 240agcacaccgt accgacagtg ggtaccgaag tcccactatg cncct 285 295 216 DNA Homo sapiens 295tcgagcggcc gcccgggcag gtccaccaca cccaattcct tgctggtatc atggcagccg 60ccacgtgcca ggattaccgg ctacatcatc aagtatgaga agcctgggtc tcctcccaga 120gaagtggtcc ctcggccccg ccctggtgtc acagaggcta ctattactgg cctggaaccg 180ggaaccgaat atacaattta tgtcattgcc ctgaag 216 296 414 DNA Homo sapiens modified_base (7) Where n is a, c, g or t 296agcgtgntcn cggccgagga tggggaagct cgnctgtctt tttccttcca atcaggggct 60nnntcttctg attattcttc agggcaanga cataaattgt atattcggnt cccggttcca 120gnccagtaat agtagcctct gtgacaccag ggcggggccg agggaccact tctctgggag 180gagacccagg cttctcatac ttgatgatga agccggtaat cctggcacgt gggcggctgc 240catgatacca ccaangaatt gggtgtggtg gacctgcccg ggcgggccgc tcgaaaancc 300gaattcntgc aagaatatcc atcacacttg ggcgggccgn tcgaaccatg catcntaaaa 360gggccccaat ttccccccta ttaggngaag ccncatttaa caaattccac ttgg 414 297 376 DNA Homo sapiens modified_base (312) Where n is a, c, g or t 297tcgagcggcc gcccgggcag gtctcgcggt cgcactggtg atgctggtcc tgttggtccc 60cccggccctc ctggacctcc tggtccccct ggtcctccca gcgctggttt cgacttcagc 120ttcctgcccc agccacctca agagaaggct cacgatggtg gccgctacta ccgggctgat 180gatgccaatg tggttcgtga ccgtgacctc gaggtggaca ccaccctcaa gagccttgag 240ccagcagaat cgaaaacatt cggaacccaa gaagggcaag cccgcaaaga aaccccgccc 300gcacctggcc gngaacctcc aagaangtgc ccacntcttg actgggaaaa aaagggaaaa 360ntacttggaa ttggac 376 298 357 DNA Homo sapiens modified_base (345) Where n is a, c, g or t 298agcgtggtcg cggccgaggt ccacatcggc agggtcggag ccctggccgc catactcgaa 60ctggaatcca tcggtcatgc tctcgccgaa ccagacatgc ctcttgtcct tggggttctt 120gctgatgtac cagttcttct gggccacact gggctgagtg gggtacacgc aggtctcacc 180agtctccatg ttgcagaaga ctttgatggc atccaggttg cagccttggt tggggtcaat 240ccagtactct ccactcttcc agtcagaagt ggcacatctt gaggtcacgg cagggtgcgg 300gcggggttct tgcgggctgc ccttctgggc tcccggaatg ttctnngaac ttgctgg 357 299 307 DNA Homo sapiens modified_base (281) Where n is a, c, g or t 299agcgtggtcg cggccgaggt ccactagagg tctgtgtgcc attgcccagg cagagtctct 60gcgttacaaa ctcctaggag ggcttgctgt gcggagggcc tgctatggtg tgctgcggtt 120catcatggag agtggggcca aaggctgcga ggttgtggtg tctgggaaac tccgaggaca 180gagggctaaa tccatgaagt ttgtggatgg cctgatgatc cacagcggag accctgttaa 240ctactacgtt gacacttgct tgtgcgccac gtgttgctca nacangggtg ggctgggcat 300caaggng 307 300 351 DNA Homo sapiens 300tcgagcggcc gcccgggcag gtctgccaag gagaccctgt tatgctgtgg ggactggctg 60gggcatggca ggcggctctg gcttcccacc cttctgttct gagatggggg tggtgggcag 120tatctcatct ttgggttcca caatgctcac gtggtcaggc aggggcttct tagggccaat 180cttaccagtt gggtcccagg gcagcatgat cttcaccttg atgcccagca caccctgtct 240gagcaacacg tggcgcacag caagtgtcaa cgtaagtaag ttaacagggt ctccgctgtg 300gatcatcagg ccatccacaa acttcatgga tttaaccctc tgtcctcgga g 351 301 330 DNA Homo sapiens 301tcgagcggcc gcccgggcag gtgtttcaga ggttccaagg tccactgtgg aggtcccagg 60agtgctggtg gtgggcacag aggtccgatg ggtgaaacca ttgacataga gactgttcct 120gtccagggtg taggggccca gctctttgat gccattggcc agttggctca gctcccagta 180cagccgctct ctgttgagtc cagggctttt ggggtcaaga tgatggatgc agatggcatc 240cactccagtg gctgctccat ccttctcgga cctgagagag gtcagtctgc agccagagta 300cagagggcca acactggtgt tctttgaata 330 302 317 DNA Homo sapiens modified_base (129) Where n is a, c, g or t 302agcgtggtcg cggccgaggt ctgtactggg agctaagcaa actgaccaat gacattgaag 60agctgggccc ctacaccctg gacaggaaca gtctctatgt caatggtttc acccatcaga 120gctctgtgnc caccaccagc actcctggga cctccacagt ggatttcaga acctcaggga 180ctccatcctc cctctccagc cccacaatta tggctgctgg ccctctcctg gtaccattca 240ccctcaactt caccatcacc aacctgcagt atggggagga catgggtcac cctgnctcca 300ggaagttcaa caccaca 317 303 283 DNA Homo sapiens modified_base (139) Where n is a, c, g or t 303tcgagcggcc gcccggacag gtctgggcgg atagcaccgg gcatattttg gaatggatga 60ggtctggcac cctgagcagt ccagcgagga cttggtctta gttgagcaat ttggctagga 120ggatagtatg cagcacggnt ctgagnctgt gggatagctg ccatgaagta acctgaagga 180ggtgctggct ggtangggtt gattacaggg ttgggaacag ctcgtacact tgccattctc 240tgcatatact ggttagtgag gtgagcctgg ccctcttctt ttg 283 304 72 DNA Homo sapiens modified_base (59) Where n is a, c, g or t 304agcgtggtcg cggccgaggt gagccacagg tgaccggggc tgaagctggg gctgctggnc 60ctgctggtcc tg 72 305 245 DNA Homo sapiens modified_base (5) Where n is a, c, g or t 305cagcngctcc nacggggcct gngggaccaa caacaccgtt ttcaccctta ggccctttgg 60ctcctctttc tcctttagca ccaggttgac cagcagcncc ancaggacca gcaaatccat 120tggggccagc aggaccgacc tcaccacgtt caccagggct tccccgagga ccagcaggac 180cagcaggacc agcagcccca gcttcgcccc ggtcacctgt ggctcacctc ggccgcgacc 240acgct 245 306 246 DNA Homo sapiens modified_base (144) Where n is a, c, g or t 306tcgagcggtc gcccgggcag gtccaccggg atagccgggg gtctggcagg aatgggaggc 60atccagaacg agaaggagac catgcaaagc ctgaacgacc gcctggcctc ttacctggac 120agagtgagga gcctggagac cganaaccgg aggctggana gcaaaatccg ggagcacttg 180gagaagaagg gaccccaggt caagagactg gagccattac ttcaagatca tcgagggacc 240tggagg 246 307 333 DNA Homo sapiens modified_base (5) Where n is a, c, g or t 307agcgnggtcg cggccgaggt ccagctctgt ctcatacttg actctaaagt catcagcagc 60aagacgggca ttgtcaatct gcagaacgat gcgggcattg tccgcagtat ttgcgaagat 120ctgagccctc aggtcctcga tgatcttgaa gtaatggctc cagtctctga cctggggtcc 180cttcttctcc aagtgctccc ggattttgct ctccagcctc cggttctcgg tctccaggct 240cctcactctg tccaggtaag aaggcccagg cggtcgttca ggctttgcat ggtctccttc 300tcgttctgga tgcctcccat tcctgccaga ccc 333 308 310 DNA Homo sapiens 308tcgagcggcc gcccgggcag gtcaggaagc acattggtct tagagccact gcctcctgga 60ttccacctgt gctgcggaca tctccaggga gtgcagaagg gaagcaggtc aaactgctca 120gatcagtcag actggctgtt ctcagttctc acctgagcaa ggtcagtctg cagccagagt 180acagagggcc aacactggtg ttcttgaaca agggcttgag cagaccctgc agaaccctct 240tccgtggtgt tgaacttcct ggaaaccagg gtgttgcatg tttttcctca taatgcaagg 300ttggtgatgg 310 309 429 DNA Homo sapiens 309agcgtggtcg cggccgaggt ccacatcggc agggtcggag ccctggccgc catactcgaa 60ctggaatcca tcggtcatgc tctcgccgaa ccagacatgc ctcttgtcct tggggttctt 120gctgatgtac cagttcttct gggccacact gggctgagtg gggtacaccg caggtctcac 180cagtctccat gttgcagaag actttgatgg catccaggtt gcagccttgg ttggggtcaa 240tccagtactc tccactcttc cagtcagaag tgggcacatc ttgaggtcac cggcaggtgc 300cgggccgggg gttcttgcgg cttgccctct gggctccgga tgttctcgat ctgcttggct 360caggctcttg agggtgggtg tccacctcga ggtcacggtc accgaaacct gcccgggcgg 420cccgctcga 429 310 430 DNA Homo sapiens modified_base (342) Where n is a, c, g or t 310tcgagcggtc gcccgggcag gtttcgtgac cgtgacctcg aggtggacac caccctcaag 60agcctgagcc agcagatcga gaacatccgg agcccagagg gcagccgcaa gaaccccgcc 120cgcacctgcc gtgacctcaa gatgtgccac tctgactgga agagtggaga gtactggatt 180gaccccaacc aaggctgcaa cctggatgcc atcaaagtct tctgcaacat ggagactggt 240gagacctgcg tgtaccccac tcagcccagt gtgggcccag aagaaactgg tacatcagca 300aggaacccca aggacaagag gcattgtctt ggttcggcga gnagcatgac ccgatggatt 360ccagtttcga gtattggcgg ccagggcttc ccgacccttg ccgatgtgga cctcggccgc 420gaccaccgct 430

Claims

1. An isolated polynucleotide comprising a sequence selected from the group consisting of SEQ ID NO:76, 77, 78, and 81.

2. An isolated polynucleotide consisting of a sequence selected from the group consisting of SEQ ID NO: 76, 77, 78, and 81.

3. An isolated polynucleotide useful in the detection of ovarian cancer comprising at least 30 contiguous nucleotide residues of SEQ ID NO:77.

4. An isolated polynucleotide useful in the detection of ovarian cancer consisting of at least 30 and not more than 361 contiguous nucleotide residues of SEQ ID NO:77.

5. An isolated polynucleotide fully complementary to a polynucleotide according to any one of claims 1, 2, 3, and 4.

6. A vector comprising a polynucleotide according to any one of claims 1, 2, 3, and 4.

7. An isolated host cell comprising a vector according to claim 6.

8. An isolated polynucleotide comprising SEQ ID NO: 74.

9. An isolated polynucleotide consisting SEQ ID NO: 74.

10. An isolated polynucleotide fully complementary to a polynucleotide according to any one of claims 8 and 9.

11. A vector comprising a polynucleotide according to any one of claims 8 and 9.

12. An isolated host cell comprising a vector according to claim 11.