Claims
- 1. An isolated polynucleotide molecule comprising a sequence of nucleotides that encode a polypeptide as shown in SEQ ID NO: 2 from residue 32 to residue 162.
- 2. The isolated polynucleotide molecule of claim 1, wherein the nucleotides are as shown in SEQ ID NO: 1 from nucleotide 140 to nucleotide 532 or as shown in SEQ ID NO: 3 from nucleotide 94 to nucleotide 486.
- 3. An isolated polynucleotide molecule comprising a sequence of nucleotides that encode a polypeptide as shown in SEQ ID NO: 2 from residue 1 to residue 162.
- 4. The isolated polynucleotide molecule of claim 3, wherein the nucleotides are as shown in SEQ ID NO: 1 from nucleotide 47 to nucleotide 532 or as shown in SEQ ID NO: 3 from nucleotide 1 to nucleotide 486.
- 5. An expression vector comprising the following operably linked elements:(a) a transcription promoter; (b) a DNA segment encoding a polypeptide comprising amino acid residues 32 (Gln) to 162 (Ser) of SEQ ID NO: 2; and (c) a transcription terminator.
- 6. A cultured cell comprising the expression vector according to claim 5.
- 7. A method of producing a polypeptide comprising culturing a cell according to claim 6 under conditions wherein the DNA segment is expressed and recovering the polypeptide encoded by the DNA segment.
- 8. An isolated polynucleotide molecule comprising a sequence of nucleotides that encode an isolated polypeptide comprising a sequence of amino acid residues that is at least 90% identical to residues 41 (Gln) to 148 (Ile) as shown in SEQ ID NO:2, wherein the residue at position 44 is Asp, the residue at position 47 is Asp and the residue at position 135 is Glu, wherein the polypeptide binds a receptor as shown in SEQ ID NO:115.
- 9. The isolated polynucleotide molecule of claim 8, wherein the nucleotides are as shown in SEQ ID NO: 1 from nucleotide 167 to nucleotide 490 or as shown in SEQ ID NO: 3 from nucleotide 121 to nucleotide 444.
- 10. The polynucleotide molecule of claim 8, wherein the polynucleotide molecule encodes a polypeptide that stimulates proliferation of NK cells or NK cell progenitors, stimulates activation of NK cells, stimulates proliferation of T cells, stimulates proliferation of B cells stimulated with anti-CD40 antibodies, or reduces proliferation of B cells stimulated with anti-IgM antibodies.
- 11. An isolated polynucleotide molecule comprising a sequence of nucleotides that encode for an isolated polypeptide comprising at least 14 contiguous amino acid residues of SEQ ID NO:2 or SEQ ID NO:56, wherein said polypeptide stimulates an antigenic response in a mammal.
- 12. An expression vector comprising the following operably linked elements:(a) a transcription promoter; (b) a DNA segment encoding a polypeptide comprising a sequence of amino acid residues selected from the group consisting of: (a) amino acid residues 41-56 of SEQ ID NO: 2; (b) amino acid residues 69-84 of SEQ ID NO: 2; (c) amino acid residues 92-105 of SEQ ID NO: 2; and (d) amino acid residues 135-148 of SEQ ID NO: 2; wherein said amino acid sequence stimulates an antigenic response in a mammal; and (c) a transcription terminator.
- 13. A cultured cell comprising the expression vector according to claim 12.
- 14. A method of producing a polypeptide comprising culturing a cell according to claim 13 under conditions wherein the DNA segment is expressed and recovering the polypeptide encoded by the DNA segment.
- 15. An expression vector comprising the following operably linked elements:(a) a transcription promoter; (b) a DNA segment encoding a polypeptide comprising a sequence of amino acid residues that is at least 90% identical to residues 41 (Gln) to 148 (Ile) as shown in SEQ ID NO: 2, wherein the residue at position 44 is Asp, the residue at position 47 is Asp and the residue at position 135 is Glu, wherein the polypeptide binds a receptor as shown in SEQ ID NO:115; and (c) a transcription terminator.
- 16. A cultured cell comprising the expression vector according to claim 15.
- 17. A method of producing a polypeptide comprising culturing a cell according to claim 16 under conditions wherein the DNA segment is expressed and recovering the polypeptide encoded by the DNA segment.
- 18. An isolated polynucleotide comprising a sequence nucleotides that encode amino acid residues that are identical to residues 41 (Gln) to 148 (Ile) as shown in SEQ ID NO:2.
- 19. An expression vector comprising the following operably linked element:(a) a transcription promoter; (b) a DNA segment comprising a nucleic acid sequence that encodes amino acid residues according to claim 18; and (c) a transcription terminator.
- 20. A cultured cell comprising the expression vector according to claim 19.
- 21. An isolated polynucleotide molecule comprising a sequence of nucleotides that encode a polypeptide as shown in SEQ ID NO:56 from residue 23 to residue 146.
- 22. An isolated polynucleotide molecule comprising a sequence of nucleotides that encode a polypeptide as shown in SEQ ID NO:56 from residue 1 to residue 146.
- 23. An isolated polynucleotide molecule comprising a sequence of nucleotides that encode for an isolated polypeptide comprising at 14 contiguous amino acid residues of SEQ ID NO:56, wherein said 14 contiguous amino acid residues of SEQ ID NO: 56 stimulates an antigenic response in a mammal.
- 24. An isolated polynucleotide molecule comprising a sequence of nucleotides that encode a polypeptide as shown in SEQ ID NO:56 from residue 23 to residue 146.
REFERENCE TO RELATED APPLICATIONS
This application is a divisional of U.S. Ser. No. 09/522,217, filed Mar. 9, 2000, issued as U.S. Pat. No. 6,307,024, on Oct. 23, 2001; and is related to Provisional Applications 60/123,547, filed on Mar. 9, 1999; No. 60/123,904, filed on Mar. 11, 1999; and No. 60/142,013, filed on Jul. 1, 1999; for which claims of benefit are made under 35 U.S.C. §§119(e)(1) and 120.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
6057128 |
Donaldson et al. |
May 2000 |
A |
Non-Patent Literature Citations (2)
Entry |
Parrish-Novak et al., Nature 408: 57-63, 2000. |
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Provisional Applications (3)
|
Number |
Date |
Country |
|
60/123547 |
Mar 1999 |
US |
|
60/123904 |
Mar 1999 |
US |
|
60/142013 |
Jul 1999 |
US |