Claims
- 1. An isolated nucleic acid segment encoding the amino acid sequence of SEQ ID NO:19.
- 2. The nucleic acid segment of claim 1, defined further as comprising a contiguous nucleotide sequence consisting of SEQ ID NO:9.
- 3. The nucleic acid segment of claim 1, defined further as a recombinant vector.
- 4. The nucleic acid segment of claim 3, defined further as an expression vector and wherein said expression vector comprises a promoter operatively linked to said nucleic acid segment.
- 5. A recombinant host cell containing the recombinant vector of claim 4.
- 6. The recombinant host cell of claim 4, defined further as a prokaryotic cell.
- 7. The recombinant host cell of claim 4, defined further as a eukaryotic cell.
- 8. A nucleic acid segment comprising at least a 14 nucleotide contiguous sequence corresponding to, or complementary to, a 14 nucleotide contiguous sequence of SEQ ID NO:9.
- 9. The nucleic acid segment of claim 8, defined further as comprising a 20 nucleotide contiguous sequence corresponding to, or complementary to, a 20 nucleotide contiguous sequence of SEQ ID NO:9.
- 10. The nucleic acid segment of claim 8, defined further as comprising a 30 nucleotide contiguous sequence corresponding to, or complementary to, a 30 nucleotide contiguous sequence of SEQ ID NO:9.
- 11. The nucleic acid segment of claim 8, defined further as comprising a 50 nucleotide contiguous sequence corresponding to, or complementary to, a 50 nucleotide contiguous sequence of SEQ ID NO:9.
- 12. The nucleic acid segment of claim 8, defined further as comprising a 100 nucleotide contiguous sequence corresponding to, or complementary to, a 100 nucleotide contiguous sequence of SEQ ID NO:9.
- 13. The nucleic acid segment of claim 8, defined further as comprising a 200 nucleotide contiguous sequence corresponding to, or complementary to, a 200 nucleotide contiguous sequence of SEQ ID NO:9.
- 14. The nucleic acid segment of claim 8, defined further as comprising a 500 nucleotide contiguous sequence corresponding to, or complementary to, a 500 nucleotide contiguous sequence of SEQ ID NO:9.
- 15. The nucleic acid segment of claim 8, defined further as comprising a 647 nucleotide contiguous sequence corresponding to, or complementary to, the nucleic acid sequence of SEQ ID NO:9.
- 16. The nucleic acid segment of claim 8, defined further as consisting of the nucleic acid sequence of SEQ ID NO:9 or its complement.
- 17. The nucleic acid segment of claim 8, defined further as less than 10,000 nucleotides in length.
- 18. The nucleic acid segment of claim 8, defined further as less than 5,000 nucleotides in length.
- 19. The nucleic acid segment of claim 8, defined further as less than 3,000 nucleotides in length.
- 20. The nucleic acid segment of claim 8, defined further as less than 1,000 nucleotides in length.
- 21. The nucleic acid segment of claim 8, defined further as less than 500 nucleotides in length.
- 22. The nucleic acid segment of claim 8, defined further as less than 100 nucleotides in length.
- 23. The nucleic acid segment of claim 8, defined further as a DNA segment.
- 24. The nucleic acid segment of claim 8, defined further as comprising a detectable label.
- 25. A method for detecting a cell converted to mucoidy, comprising the steps of:
- (a) obtaining a cell sample suspected of conversion to mucoidy;
- (b) contacting messenger RNA from said cell sample with a nucleic acid segment comprising a contiguous sequence consisting of at least a 14 nucleotide contiguous sequence of SEQ ID NO:9 or its complement under high stringency conditions; and
- (c) identifying the presence of hybridized complexes;
- wherein the presence of a hybridized complex is indicative of conversion to mucoidy.
- 26. The method of claim 25, wherein the nucleic acid segment comprises a detectable label and hybridized complexes are detected by detecting said label.
- 27. The method of claim 25, wherein said nucleic acid segment comprises a radio-, an enzymatic-, a fluorescent-, a biotinyl-, or a chemiluminescent-label.
Parent Case Info
The present application is a continuation-in-part of U.S. patent application Ser. No. 08/017,114, filed Feb. 12, 1993, and of PCT Application No. PCT/US94/02034, filed Feb. 14, 1994. The entire text of the above-referenced disclosures is specifically incorporated by reference herein without disclaimer.
Government Interests
The government owns certain rights in the present invention pursuant to grant number AI31139 from the National Institutes of Health.
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Continuation in Parts (1)
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Number |
Date |
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Parent |
17114 |
Feb 1993 |
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