Claims
- 1. A method, comprising:
a) generating a light beam; b) redirecting said light beam with a micromirror such that it is directed to a reaction site under conditions such that a photogenerated reaction takes place, said reaction site comprising a compound bound to a substrate.
- 2. The method of claim 1, wherein said light beam is redirected with a plurality of micromirrors.
- 3. The method of claim 2, wherein said micromirrors are individual rocking mirrors.
- 4. The method of claim 2, further comprising prior to step b) controlling said micromirrors with a computer.
- 5. A method of deprotecting initiating moieties attached to a solid surface comprising:
a) providing initiating moieties at a plurality of specific reaction sites on a solid surface; b) contacting at least a portion of said initiating moieties with a liquid solution comprising one or more photo-reagent precursors, said precursors selected from the group consisting of acid precursors and base precursors; and c) exposing said solid surface to a desired light pattern so as to cause formation of a photo-reagent from said photo-reagent precursor, said photo-reagent directly deprotecting said initiating moieties so as to create deprotected initiating moieties.
- 6. The method of claim 5, wherein said desired light pattern is generated using micromirrors.
- 7. The method of claim 6, wherein said micromirrors are individual rocking mirrors.
- 8. The method of claim 5, wherein after step c) a predetermined set of said specific reaction sites are unexposed to said desired light pattern.
- 9. A method of deprotecting initiating moieties attached to a solid surface comprising:
a) providing:
i) initiating moieties at a plurality of specific reaction sites on a solid surface; and ii) a system, comprising a light source and a plurality of mirrors, configured to generate a desired light pattern; b) contacting at least a portion of said initiating moieties with a liquid solution comprising one or more photo-reagent precursors, said precursors selected from the group consisting of acid precursors and base precursors; and c) exposing said solid surface to said desired light pattern so as to cause formation of a photo-reagent from said photo-reagent precursor, said photo-reagent directly deprotecting said initiating moieties so as to create deprotected initiating moieties.
- 10. The method of claim 9, wherein said mirrors are micromirrors.
- 11. The method of claim 10, wherein said mirrors are individual rocking mirrors.
- 12. The method of claim 9, wherein said system further comprises a computer configured for controlling said mirrors.
- 13. The method of claim 9, wherein after step c) a predetermined set of said specific reaction sites are unexposed to said desired light pattern.
- 14. The method of claim 9, wherein said initiating moieties comprise linker molecules, each of said linker molecules comprising a reactive functional group protected by an acid-labile protecting group.
- 15. A method of deprotecting initiating moieties attached to a solid surface comprising:
a) providing:
i) initiating moieties at a plurality of specific reaction sites on a solid surface; and ii) an optical arrangement, comprising;
a plurality of mirrors; a light source configured to generate a light beam, a filter in-line with said light source configured to generate a desired light wavelength, a condenser lens in-line with said filter configured to focus said desired light wavelength, a reflector in-line with said condenser lens configured to redirect said desired light wavelength onto said plurality of mirrors so as to generate a desired light pattern, a projection lens in-line with said mirrors configured to expose said solid surface to said desired light pattern; b) contacting at least a portion of said initiating moieties with a liquid solution comprising one or more photo-reagent precursors, said precursors selected from the group consisting of acid precursors and base precursors; and c) exposing said solid surface to said desired light pattern so as to cause formation of a photo-reagent from said photo-reagent precursor, said photo-reagent directly deprotecting said initiating moieties so as to create deprotected initiating moieties.
- 16. The method of claim 15, wherein said mirrors are individual rocking mirrors.
- 17. The method of claim 16, further comprising prior to step c) controlling said mirrors with a computer.
- 18. The method of claim 15, wherein after step c) a predetermined set of said specific reaction sites are unexposed to said desired light pattern.
- 19. A method of deprotecting initiating moieties attached to a solid surface comprising:
a) contacting said initiating moieties with a liquid solution comprising one or more photo-reagent precursors, said precursors selected from the group consisting of acid precursors and base precursors; and b) irradiating said liquid solution to cause formation of a photo-reagent from said photo-reagent precursor, said photo-reagent directly deprotecting said initiating moieties so as to create deprotected initiating moieties.
- 20. The method of claim 19 wherein said liquid solution further comprises photosensitizers for enhancing the conversion of said photo-reagent precursors.
- 21. The method of claim 19 wherein said liquid solution further comprises stabilizers for improving the yield of said deprotected initiating moieties.
- 22. A method of deprotecting initiating moieties attached to a solid surface comprising:
a) irradiating a liquid solution comprising one or more photo-reagent precursors to cause conversion of said photo-reagent precursors; and b) contacting the irradiated liquid solution with said solid surface under conditions such that said solution is contained within defined areas on said solid surface, thereby deprotecting the initiating moieties.
- 23. The method of claim 19 or 22 wherein said irradiating of step (a) produces protons.
- 24. The method of claim 23 wherein said protons contact said initiating moieties by diffusion.
- 25. A method of simultaneously deprotecting initiating moieties at a plurality of specific reaction sites on a substrate, said initiating moieties being attached to said substrate, the method comprising:
a) contacting a liquid solution comprising one or more photo-reagent precursors with said substrate such that said liquid solution is in contact with said initiating moieties; b) isolating said specific reaction sites; and c) irradiating a selected number of the isolated reaction sites to produce photo-generated reagents thereby simultaneously deprotecting said initiating moieties at the irradiated reaction sites.
- 26. The method of claim 25 wherein said specific reaction sites are isolated using isolation mechanisms selected from the group consisting of reaction wells, reactor cartridges and non-wetting surfaces.
- 27. The method of claim 25 wherein said selected number of isolated reaction sites is irradiated under computer control.
- 28. The method of claim 27 wherein said computer control comprises a programmable light projection device comprising a programmable spatial optical modulator.
- 29. The method of claim 28 wherein said programmable spatial optical modulator is selected from the group consisting of digital micromirror device, reflective liquid crystal display device, and transmissive liquid crystal display device.
- 30. The method of claim 26 wherein said production of photo-generated reagent at a selected number of isolated reaction sites is controlled by the light dosage applied to each irradiated reaction site.
Parent Case Info
[0001] This application claims the benefit of U.S. Provisional Application No. 60/074,368, filed on Feb. 11, 1998.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60074368 |
Feb 1998 |
US |
Continuations (2)
|
Number |
Date |
Country |
Parent |
10157442 |
May 2002 |
US |
Child |
10241411 |
Sep 2002 |
US |
Parent |
09248093 |
Feb 1999 |
US |
Child |
10157442 |
May 2002 |
US |