Claims
- 1. A protein aggregate containing Cu.sup.++ ions obtained by the process comprising:
- (a) providing a host cell that expresses said desired protein;
- (b) culturing said host cell in a medium comprising an effective amount of Cu++ wherein said desired protein is expressed as an inclusion body;
- (c) disrupting said host cell to produce a cell lysate having a soluble fraction and an insoluble fraction; and
- (d) separating said soluble fraction from said insoluble fraction, wherein said insoluble fraction comprises the protein aggregate.
- 2. The protein aggregate of claim 1, wherein said host cell is yeast.
- 3. The protein aggregate of claim 2, wherein said yeast is of the genus Saccharomyces.
- 4. The protein aggregate of claim 1, wherein said desired protein is selected from the group consisting of SOD/E1, SOD/PI, SOD/PI/EI, env2-3, c25, and SOD/NS5.
- 5. The protein aggregate of claim 1, wherein said host cell is Escherichia coli.
- 6. The protein aggregate of claim 1, wherein said desired protein is selected from the group consisting of IL-2, IFN-.beta., M-CSF, and c33c.
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation of application Ser. No. 07/869,613, filed 16 Apr. 1992, now U.S. Pat No. 5,523,215 which is a continuation-in-part of copending U.S. patent application Ser. No. 07/680,046 filed Mar. 29, 1991, now U.S. Pat. No. 5,342,921, which is a continuation of U.S. patent application Ser. No. 07/169,833 filed Mar. 17, 1988, now abandoned, which is a divisional of U.S. patent application Ser. No. 06/845,737 filed Mar. 28, 1986 now U.S. Pat. No. 4,751,180, which is a continuation-in-part of U.S. patent application Ser. No. 717,209 filed Mar. 28, 1985, now abandoned. All of the referenced applications are incorporated herein by reference in full.
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Divisions (1)
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Number |
Date |
Country |
Parent |
845737 |
Mar 1986 |
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Continuations (1)
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Number |
Date |
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Parent |
869613 |
Apr 1992 |
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Continuation in Parts (3)
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Number |
Date |
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Parent |
680046 |
Mar 1991 |
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Parent |
169833 |
Mar 1988 |
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Parent |
717209 |
Mar 1985 |
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