Claims
- 1. A process of fermenting a phytic acid-containing material, comprising fermenting said phytic acid-containing material in the presence of a phytase.
- 2. The process of claim 1, wherein said phytase is derived from a strain of Peniophra lycii, Aspergillus oryzae, Aspergillus ficumm, or Aspergillus niger.
- 3. The process of claim 1, wherein said phytase has a temperature optimum in the range of 20-70° C.
- 4. The process of claim 1, wherein said phytase has a temperature optimum of above 50° C.
- 5. The process of claims 1, wherein said phytic acid-containing material is fermented with a micro-organism capable of fermenting sugars or converted sugars.
- 6. The process of claim 5, wherein said micro-organism is a yeast.
- 7. The process of claim 5, wherein said micro-organism is Saccharomyces cerevisae.
- 8. The process of claim 1, wherein said phytic acid-containing material is derived from whole grains or a side stream from starch processing.
- 9. The process of claim 1, wherein said phytic acid-containing material is derived from liquefied starch with a DE of 8-10.
- 10. The process of claim 1, wherein said fermentation is carried out in the presence of a carbohydrate-generating enzyme.
- 11. The process of claim 10, wherein said carbohydrate-generating enzyme is a glucoamylase.
- 12. The process of claim 1, wherein said fermentation is carried out in the presence of a protease.
- 13. The process of claim 12, wherein said protease is a fungal protease.
- 14. The process of claim 12, wherein said protease is derived from a strain of Aspergillus.
- 15. The process of claim 12, wherein said protease is derived from Aspergillus niger.
- 16. The process of claim 12, wherein said protease is a bacterial protease.
- 17. The process of claim 12, wherein said protease is an acidic, neutral or alkaline protease.
- 18. The process of claim 12, wherein said protease is derived from a strain of Bacillus.
- 19. The process of claim 1, wherein the product of said fermentation is ethanol.
- 20. A process for the production of ethanol, comprising the steps of:
(a) milling whole grains, (b) liquefying the product of said (a)in the presence of an alpha-amylase, (c) saccharifying the liquefied material obtained in said (b), and (d) fermenting the saccharified material obtained in said (c) using a micro-organism.
- 21. The process of claim 20, further comprising (e) distilling the fermented and saccharified material obtained in step (d).
- 22. The process of claim 20, further comprising adding a carbohydrate-source generating enzyme in said (c).
- 23. The process of claim 22, wherein said carbohydrate-source generating enzyme is a glucoamylase.
- 24. The process of claim 20, wherein said (b) comprises:
i) heating a hot slurry comprising milled whole grains from said (a) to between 60-95° C. and adding at least one alpha-amylase to said slurry; ii) jet-cooking said slurry at a temperature between 95-140° C. to complete gelanitization of said slurry; and iii) cooling said slurry to 60-95° C. and adding alpha-amylase to complete hydrolysis.
- 25. The process of claim 20, wherein said (b) is carried out at pH of 4.5-6.5.
- 26. The process of claim 20, wherein said (b) is carried out at a pH between 5 and 6.
- 27. The process of claim 20, wherein a phytase is added during said (c) or prior to said (c) (pre-saccharification).
- 28. The process of claim 20, wherein a phytase is added during said (c) and/or said (d).
- 29. The process of claim 20, wherein a phytase is added during said (c).
- 30. The process of claim 20, wherein a phytase is added during said (d).
- 31. The process of claim 20, wherein said (c) and said (d) are carried out simultaneously.
- 32. The process of claim 20, wherein said (c) and said (d) are carried out sequentially.
- 33. The process of claim 21, further comprising recovering the ethanol after said (e).
- 34. The process of claim 20, wherein said (a) is a dry milling step or wet milling step.
- 35. The process of claim 20, wherein said alpha-amylase is derived from a strain of the genus Bacillus or a strain of Aspergillus.
- 36. The process of claim 28, wherein said phytase is derived from a strain of Peniophra lycii or Aspergillus oryzae
- 37. The process of claims 20, wherein said (d) is carried out using a micro-organism capable of fermenting sugars to ethanol.
- 38. The process of claim 37, wherein said micro-organism is a yeast.
- 39. The process of claim 37 wherein said micro-organism is Saccharomyces cerevisae.
- 40. The process of claim 20, wherein said (d) is carried out in the presence of carbohydrate-source generating enzyme.
- 41. The process of claim 40, wherein said carbohydrate-source generating enzyme is a glucoamylase.
- 42. The process of claim 20, wherein said (d) is carried out in the presence of a protease.
- 43. The process of claim 42, wherein said protease is a fungal protease.
- 44. The process of claim 42, wherein said protease is an acid fungal protease derived from a strain of Aspergillus.
- 45. The process of claim 42, wherein said protease is an acid fungal protease derived from a strain of Aspergillus niger.
- 46. The process of claim 42, wherein said protease is an acid, neutral or alkaline protease.
- 47. The process of claim 42, wherein said protease is derived from a strain of Bacillus.
- 48. A process for the production of ethanol, comprising the steps of:
(a) providing a phytic acid-containing material, (b) liquefying said phytic acid-containing material in the presence of an alpha-amylase, (c) saccharifying the liquefied material obtained in said (b), and (d) fermenting the saccharified material obtained in said (c) using a micro-organism.
- 49. The process of claim 48, wherein the phytic acid-containing material is milled whole grain or a side stream from starch processing.
- 50. A composition comprising at least one phytase and at least one carbohydrate-generating enzyme.
- 51. The composition of claim 50, wherein said at least one carbohydrate-generating enzyme is a glucoamylase.
- 52. The composition of claim 51, further comprising a protease.
- 53. The composition of claim 52, wherein said protease is an acid protease.
- 54. The composition of claim 52, wherein said protease is an acid fungal protease.
Priority Claims (1)
Number |
Date |
Country |
Kind |
PA 2000 00281 |
Feb 2000 |
DK |
|
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation of U.S. application Ser. No. 09/788,906, filed on Feb. 20, 2001, and claims the benefit of U.S. Provisional application No. 60/185,716, filed Feb. 23, 2000, and priority under 35 U.S.C. 119 of Danish application PA 2000 00281, filed Feb. 23, 2000, the contents of which are fully incorporated herein by reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60185716 |
Feb 2000 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09788906 |
Feb 2001 |
US |
Child |
10164029 |
Jun 2002 |
US |