1. Technical Field
The present invention relates to optical scanning devices in general, and in particular to a frequency turnable micromirror to be utilized in an optical scanning device.
2. Description of Related Art
A laser-based scanning fluorescence imaging system typically includes a confocal probe having a micromirror and an objective lens. The field of view (FOV) of the confocal probe is determined by the back aperture size of the objective lens. In addition, the numerical aperture (NA) of the objective lens determines the fluorescence photon collection ability of the confocal probe. While conducting macrofield imaging using the confocal probe, the reflecting area of a micromirror is the limiting factor for fully utilizing the photon collected by the objective lens.
The above-mentioned problem can be addressed by switching the fluorescence beam onto another light path and by scaling down the beam before descanning it using another micromirror. This solution guarantees that the power efficiency of the imaging system will be decoupled with the surface area of the micromirror while requiring the synchronization of the two micromirrors in terms of scanning frequency, amplitude and phase. For a micro scanner to achieve certain effective scanning amplitude, the mirror scanner needs to work under resonant scanning mode. Among these factors, frequency tuning is more critical due to its relevance to the natural response frequency of the micromirror, and therefor imaging frame rate.
The present invention enables the tuning of the resonant frequency of a micromirror in order to provide synchronization between two micromirrors within a single optical imaging system. In accordance with a preferred embodiment of the present invention, each of the two micromirrors can be rotated by a first set of combdrive actuators along a first axis and a second set of combdrive actuators along a second axis. Each of the first and second set of combdrive actuators includes multiple stator comb fingers and multiple rotor comb fingers capable of rotating about a torsion bar. One of the micromirrors can be tuned by applying a current to the torsion bar to change the Young's modulus of the torsion bar via thermoelectrical heating of the torsion bar.
All features and advantages of the present invention will become apparent in the following detailed written description.
The invention itself, as well as a preferred mode of use, further objects, and advantages thereof, will best be understood by reference to the following detailed description of an illustrative embodiment when read in conjunction with the accompanying drawings, wherein:
a is a detailed diagram of a micromirror within the confocal microscope from
b is a detailed diagram of a frequency tunable micromirror within the confocal microscope from
a-4i illustrates a method for fabricating the micromirror from
Referring now to the drawings and in particular to
A linearly-polarized laser beam from diode laser 166 is initially coupled into a single-mode polarization maintaining (PM) fiber 168. Light exiting PM fiber 168 is then collimated by collimators 169 to a 1 mm diameter beam through a zero-order quarter wave-plate 170 whose axis is oriented at 45° to the incident polarization angle in order to convert the illumination to a circular polarization. After reflection off stationary mirror 172, the illumination is incident on micromirror 174 at 22.5° to micromirror 174 normal. Micromirror 174 scans the illumination across objective system 111, providing an effective numerical aperture of about 0.48 at a tissue sample 180. Reflected light is subsequently converted into a linear polarization that is orthogonal to the initial illumination polarization, which is isolated using a walk-off polarizer 182 and an offset mirror 184, and directed through a spatial filter 186 into avalanche photodetector 188.
Higher values of numerical aperture of objective system 111 can be used to obtain better optical sectioning with high contrast in highly scattering tissue sample 180. The resolution, field of view, and contrast of confocal microscope 100 is largely determined by micromirror 174. There is, however, a trade-off in selecting between resolution and field of view. The product of micromirror 174's size and its optical deflection angle determines the number of resolvable points in the final image, which translates into a given field of view and resolution according to the numerical aperture of objective system 111.
The number of resolvable points, N, for micromirror 174 in a one-dimensional scan is given by
where θ is the mechanical scanning half-angle of micromirror 174, λ is the operating wavelength, and D is the diameter of micromirror 174.
Preferably, confocal microscope 100 can be used to provide images of a 200×125 μm field of view at 3.0 frames per second. The number of resolvable points (408×255) in the images is proportional to the product of the diameter of micromirror 174 and the optical scan angle, as stated in Equation (1). Micromirrors with larger diameters (˜1 mm) capable of providing the same deflection angles can be designed within the limits set by the maximum driving voltage and at the cost of increased energy consumption.
With reference now to
The performance of micromirror 174 is characterized by its response to various electrical signal inputs. For example, one input can be a sinusoidal variable-frequency voltage with suitable offset (to ensure the applied voltage was always positive) between ground and one of combdrive actuators 260, 262 of each rotation axis. Optical scan angles of 22° and 12° on the inner and outer axes are achieved for frequency values around 2.81 kHz and 670 Hz on the inner and outer rotation axes, respectively. On the other hand, for a static voltage applied between ground and one of combdrive actuators 260, 262 on each rotation axis, off-resonance actuation using only one combdrive actuator results in single-sided deflection. The total optical deflection angle can be doubled by making use of both combdrive actuators 260, 262 on either side of the torsion bars forming the rotation axis. In this respect, off-resonance operation differs significantly from driving at resonant frequency. Optical scan angles of about 5° and 4.5° can be achieved by applying static voltages up to 240 V on the inner and outer axes, respectively.
With reference now to
While conducting macro field imaging using laser scanning mechanism, switching a fluorescence beam onto another arm and scale down the beam before descanning it using tunable micromirror 175 ensures the number of photons collected by the collection lens system (see
Referring now to
In response to a voltage being applied at stator comb fingers 308, rotor comb fingers 306 rotate about a torsion bar 304. Specifically, when a voltage is applied at stator comb fingers 308, an electrostatic torque is experienced by rotor comb fingers 306, which subsequently rotates rotor comb fingers 306 because they are constrained primarily to rotary motion by torsion bar 304. Rotor comb fingers 306 are capable of being rotated to a maximum rotation angle of θmax. As rotor comb fingers 306 are being rotated, a shear stress is developed within torsion bar 304 due to twisting, and the shear stress offers a mechanical restoring torque against such twisting. The rotation of rotor comb fingers 306 reaches an equilibrium at a rotation angle at which the electrostatic torque exactly matches the mechanical restoring torque.
In order for micromirror 174 to achieve certain effective scanning amplitude, micromirror 174 needs to work under a resonant scanning mode. The resonant frequency of micromirror 174 can be changed by applying additional current along torsion bar 304. The current flowing through torsion bar 304 in turn causes thermal heating along torsion bar 304 and changes the Young's modulus of torsion bar 304. In other words, micromirror 174 can be electrically tuned by using thermoelectric heating of torsion bar 304 because the resonant frequency of micromirror 174 will be changed according to the change of stiffness of torsion bar 304.
A Schottky diode formed by p-type doping was utilized to assist thermal current flow in specified areas of torsion bar 304. Deposited aluminum pathway regulates the flow path of current applied to torsion bar 304 for frequency tuning. The main resistance on the pathway is determined by that of the thermal heating area on torsion bar 304 without aluminum deposition.
While synchronizing two frequency tunable micromirrors in terms of scanning frequency, amplitude and phase in a fluorescence imaging system, it is expected to realize the decoupling of the power efficiency of the imaging system with the surface area of the micromirrors.
While conducting macro field imaging using laser scanning mechanism, switching the fluorescence beam onto another atm and scale down the beam before descanning it using another micromirror ensures the number of photons collected by the collection lens system not restricted by the size of the micromirror. Thus, better imaging quality can be achieved for large scale fluorescence imaging without the cost of higher excitation input or the risk of sample bleaching and damaging effects.
With reference now to
Before further processing of wafer 400, pre-fabrication of any complementary-metal-oxide semiconductor (CMOS) circuitry can be performed at this point, if necessary. For example, CMOS circuitry may include control electronics and sensors to adaptively correct for aberrations in a micromirror.
Following the CMOS circuitry pre-fabrication (if performed), wafer 400 is cleaned by immersing wafer 400 in a 9:1 solution of H2SO4:H2O2 for approximately 8 minutes. After rinsing with de-ionized water, wafer 400 is spun dry. The above-mentioned cleaning process is commonly known as Piranha clean.
Next, wafer 400 is placed into a furnace in which a low-temperature oxide (LTO) layer 422 is deposited on top of silicon device layer 412 via a low-pressure chemical vapor deposition (LPCVD) process at a low temperature (450° C.) in order to reduce thermal budget. LTO layer 422 is preferably a silicon dioxide layer having a thickness between about 50 nm and about 1.5 μm. LTO layer 422 serves to protect any CMOS circuitry and to act as a hard mask for the deep trench etching to be performed to create vertical comb finger structures.
A first photolithography step is performed on LTO layer 422 to etch a set of coarse features 424, 426 of vertical comb finger structures on top of silicon device layer 412. The photolithography step involves coating a layer of hexamethyldisilazane (HMDS) on LTO layer 422, which serves as an adhesion promoter between LTO layer 422 and a photosensitive material to be added. Coarse features 424, 426 are etched in LTO layer 422 via a reactive ion etching (RIE) step using CHF3 and O2 gases, as shown in
A photosensitive material layer 428, such as Shipley SPR 220-3 positive photoresist, is then spun on LTO layer 422. A second photolithographic step is then performed on photosensitive material layer 428 to etch a set of fine features 430, 432 of vertical comb finger structures on top of LTO layer 422. Fine features 430, 432 are constrained laterally within respective coarse features 424, 426, as shown in
The misalignment tolerance for the second photolithography step, which includes a self-alignment step, is half of the gap spacing between stator comb fingers and rotor comb fingers. A significant advantage of the second photolithography step is that if the alignment is deemed to be unsatisfactory on inspection after the second photolithography step, the photoresist can be removed by a Piranha clean, and the self-alignment step can be repeated as many times as necessary. This flexibility eliminates the uncertainty in determining whether or not self-alignment has been achieved, as may happen when the self-alignment is performed to a layer buried deep within a material stack. The minimum comb gap spacing achievable can be determined by the maximum aspect ratio that a silicon deep reactive ion etching (DRIE) tool used in subsequent steps can achieve.
Next, a second RIE step is utilized to remove exposed LTO layer 422 in order to trim coarse features 424, 426 within LTO layer 422 to match the widths of corresponding fine features 430, 432 within photosensitive material layer 428, in order to complete the self-alignment process, as illustrated in
Using coarse features 424, 426 within LTO layer 422 and fine features 430, 432 within photosensitive material layer 428 as masks, a DRIE is utilized to remove a portion of silicon device layer 412 (stopped on silicon dioxide layer 416) to form stator comb features 438 and rotor comb features 440 on top of silicon dioxide layer 416, as shown in
A third ME step is then utilized to remove silicon dioxide layer 416, using coarse features 424, 426 within LTO layer 422 and fine features 430, 432 within photosensitive material layer 428 as masks. Photosensitive material layer 428 is subsequently removed, leaving rotor comb features 440 unprotected by any masking element, while stator comb features 438 are still protected by LTO layer 422, as illustrated in
A second DRIE step is utilized to remove portions of silicon device layers 412 and 414 (stopped on silicon dioxide layer 418) to define rotor comb fingers 444 in silicon device layer 414. After the completion of the second DRIE step, rotor comb features 444 reside only in silicon device layer 414, while stator comb features 442 reside in both silicon device layers 412 and 414, as illustrated in
The lower section of stator comb features 442 (portions located in silicon device layer 414) is redundant from an actuation perspective, but they do not affect the operation of a micromirror.
A third photolithographic step using a photoresist layer 446 is then performed on a backside of wafer 400 using a third photomask to align to the features on the front side of wafer 400. Preferably, photoresist layer 446 is approximately 15 μm thick and is capable of protecting the underlying silicon through a substrate DRIE step. Photoresist layer 446 can be, for example, Shipley SPR 220-7 positive resist. The third photomask contains the outline of a rotatable mirror structure 445 and is used to remove all silicon directly beneath rotatable mirror structure 445, as illustrated in
Since the feature on the third photomask is relatively large (comparable to the size of the entire device), a significant amount of misalignment can be tolerated. Wafer 400 is bound by photoresist to a second silicon substrate (not shown) serving as a mechanical handle in preparation for the backside substrate DRIE step on substrate 420. The backside DRIE step releases the devices and creates dicing lines to facilitate cleaving of wafer 400 into individual chips.
Wafer 400 can be separated from its handle wafer by soaking wafer 400 in acetone, following which a fourth RIE is performed on the front and back sides of wafer 400 to remove any remaining exposed hard mask in LTO layer 422 and silicon dioxide layer 418. The result is an optical scanning device having multiple bond pads 448, stator comb fingers 450, rotor comb fingers 452, and a rotatable mirror 454, as illustrated in
As a final step, metals, such as chromium/gold, can be evaporated on the surface of mirror 454 through a shadow mask to improve reflectivity.
As has been described, the present invention provides a frequency turnable micromirror. The frequency turnable micromirror of the present invention uses less power while without sacrificing optical resolution and field of view.
While the invention has been particularly shown and described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention.
The present application claims priority under 35 U.S.C. §119(e)(1) to provisional application No. 61/483,305, filed on May 6, 2011, the contents of which are incorporated herein by reference.