Claims
- 1. A method of identifying nucleic acid ligands to transforming growth factor beta (TGF.beta.) comprising:
- a) preparing a candidate mixture of nucleic acids;
- b) contacting the candidate mixture of nucleic acids with TGF.beta., wherein nucleic acids having an increased affinity to TGF.beta. relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
- c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and
- d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acid sequences with relatively higher affinity and specificity for binding to TGF.beta., whereby nucleic acid ligands of TGF.beta. may be identified.
- 2. The method of claim 1 further comprising:
- e) repeating steps b), c), and d).
- 3. The method of claim 1 wherein said candidate mixture of nucleic acids is comprised of single stranded nucleic acids.
- 4. The method of claim 3 wherein said single stranded nucleic acids are ribonucleic acids.
- 5. The method of claim 4 wherein said nucleic acids comprise 2'-amino (2'-NH.sub.2) modified ribonucleic acids.
- 6. The method of claim 4 wherein said nucleic acids comprise 2'-F modified ribonucleic acids.
- 7. The method of claim 4 wherein said nucleic acids comprise 2'-NH.sub.2 -UTP, 2'-F-CTP modified ribonucleic acids.
- 8. The method of claim 4 wherein said nucleic acids comprise 2'-F-UTP, 2'-NH.sub.2 -CTP modified ribonucleic acids.
- 9. The method of claim 3 wherein said single stranded nucleic acids are deoxyribonucleic acids.
RELATED APPLICATIONS
This application is a Continuation-in-Part of U.S. patent application Ser. No. 07/714,131, filed Jun. 10, 1991, entitled Nucleic Acid Ligands, now U.S. Pat. No. 5,475,096, U.S. patent application Ser. No. 07/931,473, filed Aug. 17, 1992, entitled Nucleic Acid Ligands, now issued as U.S. Pat. No. 5,270,163, U.S. patent application Ser. No. 07/964,624, filed Oct. 21, 1992, entitled Methods of Producing Nucleic Acid Ligands, now U.S. Pat. No. 5,496,938, and U.S. patent application Ser. No. 08/117,991, filed Sept. 8, 1993, abandoned, entitled High-Affinity Nucleic Acid Ligands Containing Modified Nucleotides. U.S. patent application Ser. No. 07/714,131 (now U.S. Pat. No. 5,475,096) is a Continuation-in-Part of U.S. patent application Ser. No. 07/536,428, filed Jun. 11, 1990, entitled Systematic Evolution of Ligands by EXponential Enrichment, now abandoned.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
5270163 |
Gold et al. |
Dec 1993 |
|
5459015 |
Janjic et al. |
Oct 1995 |
|
Foreign Referenced Citations (3)
Number |
Date |
Country |
2 183 661 |
Jun 1987 |
GBX |
WO 8906694 |
Jul 1989 |
WOX |
9214843 |
Sep 1992 |
WOX |
Non-Patent Literature Citations (17)
Entry |
Arteaga et al. (1993) J. Clin. Invest. 92:2569. |
Arteaga et al. (1990) Cell Growth & Differentiation 1:367. |
Barral et al. (1993) Proc. Natl. Acad. Sci. USA 90:3442. |
Shah et al. (1992) Lancet 339:213. |
Joyce (1989) Gene 82:83. |
Joyce and Inoue (1989) Nucleic Acids Research 17:711. |
Ellington and Szostak (1990) Abstract of papers presented at the 1990 meeting on RNA Processing, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, p. 226. |
Kinzler and Vogelstein (1989) Nucleic Acids Research 17:3645. |
Kramer et al. (1974) J. Mol. Biol. 89:719. |
Levisohn and Spiegelman (1969) Proc. Natl. Acad. Sci. USA 63:805. |
Levisohn and Spiegelman (1968) Proc. Natl. Acad. Sci. USA 60:866. |
Oliphant et al. (1989) Mol. Cell. Biol. 9:2944. |
Oliphant and Struhl (1988) Nucleic Acids Research 16:7673. |
Oliphant and Struhl (1987) Methods in Enzymology 155:568. |
Oliphant et al. (1986) Gene 44:177. |
Robertson and Joyce (1990) Nature 344:467. |
Thiesen and Bach (1990) Nucleic Acids Research 18:3203. |
Related Publications (3)
|
Number |
Date |
Country |
|
931473 |
Aug 1992 |
|
|
964624 |
Oct 1992 |
|
|
117991 |
Sep 1993 |
|
Continuation in Parts (2)
|
Number |
Date |
Country |
Parent |
714131 |
Jun 1991 |
|
Parent |
536428 |
Jun 1990 |
|