Claims
- 1. A method for identifying bioactivities or biomolecules produced by a mycelia comprising:growing a mycelia under conditions and for a time sufficient to allow the mycelia to produce a bioactivity or biomolecule that modifies a substrate molecule therein so as to cause fluorescence; fragmenting said mycelia to produce monocells; and screening said monocells using a fluorescence analyzer to identify one or more bioactivity or biomolecule produced by the mycelia.
- 2. The method of claim 1, wherein the mycelia is a recombinant cell.
- 3. The method of claim 1, wherein the mycelia is a non-recombinant cell.
- 4. The method of claim 1, wherein the biomolecule is a secondary metabolite.
- 5. The method of claim 1, wherein the cell is a filamentous bacteria or fungi.
- 6. The method of claim 1, wherein the growth conditions include liquid culture.
- 7. The method of claim 1, wherein the fragmenting comprises centrifugation of the mycelia to produce the monocells.
- 8. The method of claim 3, wherein the cell is a Streptomyces.
- 9. The method of claim 8, wherein the Streptomyces is Streptomyces venezuelae.
- 10. A method for identifying bioactivities or biomolecules produced by a mycelia comprising:growing a mycelia under conditions and for a time sufficient to allow the mycelia to produce a bioactivity or biomolecule that modifies a substrate molecule therein so as to cause fluorescence; fragmenting said mycelia to produce monocells; growing the monocells in an encapsulated system; and screening said encapsulated mycelia using a fluorescence analyzer to identify one or more bioactivity or biomolecule produced by the mycelia.
Parent Case Info
This application is a divisional of U.S. patent application Ser. No. 09/636,778, filed Aug. 11, 2000, which is a continuation of U.S. patent application Ser. No. 09/098,206, filed Jun. 16, 1998 now U.S. Pat. No. 6,174,673, continuation-in-part of U.S. application Ser. No. 08/876,276, filed Jun. 16, 1997.
US Referenced Citations (8)
Foreign Referenced Citations (7)
Number |
Date |
Country |
WO 9634112 |
Oct 1996 |
WO |
WO 9704077 |
Feb 1997 |
WO |
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Jun 1997 |
WO |
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Dec 1997 |
WO |
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Dec 1998 |
WO |
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Sep 1999 |
WO |
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Oct 1999 |
WO |
Non-Patent Literature Citations (2)
Entry |
Roessner et al., “Fluorescence-Based Method for Selection of Recombinant Plasmids,” BioTechniques, 19:760-764, Nov. 1995. |
Wittrup et al., “Microencapsulation Selection for Isolation of Yeast Mutants with Increased Secretion of Aspergillus awamori Glucoamylase,” Biotechnology and Bioengineering, 42(3):351-356, Jul. 1993. |
Continuations (1)
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Number |
Date |
Country |
Parent |
09/098206 |
Jun 1998 |
US |
Child |
09/636778 |
|
US |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
08/876276 |
Jun 1997 |
US |
Child |
09/098206 |
|
US |