Claims
- 1. A substantially pure human nucleic acid comprising at least 40 nucleotides that hybridizes under high stringency conditions to a sequence found within the nucleic acid of SEQ ID NO:1.
- 2. The nucleic acid of claim 1, wherein said sequence has a sequence complementary to at least 50% of at least 60 contiguous nucleotides of the nucleic acid encoding the methionine synthase polypeptide, said sequence sufficient to allow nucleic acid hybridization under high stringency conditions.
- 3. The nucleic acid of claim 1, wherein said nucleic acid comprises a mutation or a polymorphism, wherein said nucleic acid probe detects a mutation or polymorphism selected from the group consisting of D919G, H920D, and ΔIle881.
- 4. The nucleic acid of claim 3, wherein said sequence of said nucleic acid comprises the cobalamin binding domain of the human methionine synthase gene.
- 5. The nucleic acid of claim 2, wherein at least 18 contiguous nucleotides of said sequence are complementary to at least 90% of the corresponding nucleotides of the nucleic acid encoding the methionine synthase polypeptide.
- 6. The nucleic acid of claim 1, wherein said high stringency conditions comprise hybridization in 2×SSC at 40° C.
- 7. A substantially pure human nucleic acid, wherein the sequence of said nucleic acid is at least 75% identical to the corresponding region of at least 50 contiguous base pairs of the nucleic acid of SEQ ID NO:1.
- 8. A substantially pure human nucleic acid, wherein the sequence of said nucleic acid is at least 35% identical to the corresponding region of at least 50 contiguous base pairs of the nucleic acid of SEQ ID NO:1.
- 9. A kit for the analysis of a human methionine synthase nucleic acid, said kit comprising a nucleic acid probe useful for detecting in the nucleic acids of a human a mutation or polymorphism in said methionine synthase nucleic acid, wherein said mutation or polymorphism is selected from the group consisting of D919G, H920D, and ΔIle881.
- 10. The kit of claim 9, wherein said probe comprises at least 40 nucleotides that hybridizes at high stringency to a sequence found within the nucleic acid of SEQ ID NO:1.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This invention claims priority from U.S. Provisional Applications Serial Nos. 60/031,964 and 60/050,310, filed Nov. 27, 1996 and Jun. 20, 1997, respectively.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60031964 |
Nov 1996 |
US |
|
60050310 |
Jun 1997 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
08980326 |
Nov 1997 |
US |
Child |
10607712 |
Jun 2003 |
US |