Claims
- 1. An immunogen comprising a peptide from the CCK-B/gastrin-receptor conjugated to an Immunogenic carrier.
- 2. The immunogen of claim 1, wherein the peptide has the amino acid sequence KLNRSVQGTGPGPGASL (SEQ ID NO.: 1 in the Sequence Listing) or GPGAHRALSGAPISF (SEQ ID NO.: 2 in the Sequence Listing).
- 3. The immunogen of claim 1 or 2, further comprising a spacer peptide sequence.
- 4. The immunogen of claim 3, wherein the spacer peptide sequence is SSPPPPC (SEQ ID NO.: 3 in the Sequence Listing).
- 5. The immunogen of claim 1, wherein the immunogenic carrier is selected from the group consisting of Diphtheria toxoid, tetanus toxoid and bovine serum albumin.
- 6. A method for treating a malignant condition caused by gastrin-dependent malignant cell growth comprising administering to an animal in need of such treatment an effective amount of an anti-CCK-B/gastrin-receptor immunogen.
- 7. The method of claim 6, wherein the immunogen comprises a peptide from the CCK-B/gastrin-receptor.
- 8. The method of claim 7, wherein the peptide has the amino acid sequence KLNRSVQGTGPGPGASL (SEQ ID NO.: 1 in the Sequence Listing) or GPGAHRALSGAPISF (SEQ ID NO.: 2 in the Sequence Listing).
- 9. The method of claim 8, wherein the immunogen further comprises a spacer peptide sequence.
- 10. The method of claim 9, wherein the spacer peptide sequence is SSPPPPC (SEQ ID NO.:3 in the Sequence Listing).
- 11. The method of claim 10, further comprising an immunogenic carrier selected from the group consisting of Diptheria toxoid, tetanus toxoid and bovine serum albumin.
- 12. A method for treating a gastrin-dependent tumor comprising administering to an animal in need of such treatment an effective amount of anti-CCK-B/gastrin antibodies which recognize and bind to the CCK-B/gastrin-receptors in the tumor cells.
- 13. The method of claim 12, wherein the antibodies are selected from the group consisting of chimeric, monoclonal and humanized antibodies.
- 14. The method of claim 12, wherein the anti-CCK-B/gastrin-receptor antibodies recognize and bind the amino acid sequence KLNRSVQGTGPGPGASL (SEQ ID NO.: 1 in the Sequence Listing) or GPGAHRALSGAPISF (SEQ ID NO.: 2 in the Sequence Listing) of the receptor.
- 15. The method of claim 12 or 14, wherein the antibodies are further conjugated to a cytotoxic molecule.
- 16. The method of claim 15, wherein the cytotoxic molecule is a toxin or a radioactive molecule.
- 17. The method of claim 16, wherein the toxin is cholera toxin.
- 18. The method of claim 16, wherein the radioactive molecule is labeled with 125I or 131 I.
- 19. A method for detecting a gastrin-responsive tumor containing CCK-B/gastrin-receptors, comprising exposing an anti-gastrin-receptor antibody to cells isolated from a tumor biopsy sample and detecting the CCK-B/gastrin-receptor in the sample.
- 20. The method of claim 19, wherein the anti-gastrin-receptor antibody is specific for an amino-terminal peptide of the CCK-B/gastrin-receptor.
- 21. The method of claim 20, wherein the peptide is in the amino-terminal region of the CCK-B/gastrin-receptor comprises amino acid residues 5-21.
- 22. An immunogenic composition comprising an anti-CCK-B/gastrin-receptor immunogen.
- 23. The composition of claim 22, wherein the immunogen comprises a peptide from the CCK-B/gastrin-receptor.
- 24. The compostion of claim 22, wherein the immunogen comprises antibodies against the CCK-B/gastrin-receptor.
- 25. A method for diagnosing a gastrin-dependent tumor, comprising administering radiolabeled anti-CCK-B/gastrin-receptor antibodies to a patient possessing a colorectal tumor and imaging the tumor by scintigraphic scanning.
- 26. The method of claim 25, wherein the anti-CCK-B/gastrin-receptor antibodies are radiolabeled with 111Indium or 90Yttrium.
CROSS REFERENCE TO RELATED APPLICATION
[0001] This application claims the benefit under 35 U.S.C. §119(e), of U.S. Provisional Application No. 60/046,201 filed on May 12, 1997.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60046201 |
May 1997 |
US |
Divisions (1)
|
Number |
Date |
Country |
Parent |
09076372 |
May 1998 |
US |
Child |
10235236 |
Sep 2002 |
US |