Claims
- 1. A method of enhancing production performance of a bird, comprising administering an effective amount of a heterologous protein comprising alpha-subunit avian inhibin protein, or a fragment thereof, and a carrier protein, to a bird such that the production performance of the bird is improved.
- 2. The method of claim 1, wherein the bird is selected from the group consisting of ratites, psittaciformes, falconiformes, piciformes, strigiformes, passeriformes, coraciformes, ralliformes, cuculiformes, columbiformes, galliformes, anseriformes, and herodiones.
- 3. The method of claim 2, wherein the ratite is an ostrich, emu, rhea, kiwi, or cassowary.
- 4. The method of claim 2, wherein the bird is a chicken, turkey, parrot, parakeet, makaw, falcon, eagle, quail, hawk, pigeon, cockatoo, song bird, jay bird, blackbird, finch, warbler, goose, duck, canary, mynah, toucan, or sparrow.
- 5. The method of claim 1, wherein the inhibin protein has or contains substantially the same sequence as SEQ ID NO: 2.
- 6. The method of claim 1, wherein the carrier protein is maltose binding protein, bovine serum albumin, ovalbumin, flagellin, keyhole lympet hemocyanin, thyroglobulin, serum albumin, gamma globulin, syngeneic cells, syngeneic cells bearing Ia antigens, or polymers of amino acids.
- 7. The method of claim 1, wherein the bird is a female bird.
- 8. The method of claim 1, wherein the bird is a male bird.
- 9. A fused heterologous protein comprising alpha-subunit inhibin protein, or a fragment thereof, fused to a carrier protein.
- 10. The fused heterologous protein of claim 9, wherein the inhibin protein has or contains substantially the same sequence as SEQ ID NO: 2.
- 11. The fused heterologous protein of claim 9, wherein the carrier protein is maltose binding protein, bovine serum albumin, ovalbumin, flagellin, keyhole lympet hemocyanin, serum albumin, gamma globulin, thyroglobulin, syngeneic cells, syngeneic cells bearing Ia antigens, or polymers of amino acids.
- 12. A method of producing a fused heterologous protein comprising the steps of:
(a) providing double strand cDNA which is encoded for alpha-subunit inhibin, or a fragment thereof; (b) providing a vector which has coding information for the production of a carrier protein; (c) ligating the inhibin cDNA into the vector; (d) inserting the ligated vector into an expression system; and (e) expressing a fused heterologous protein comprising inhibin protein, or a fragment thereof, and the carrier protein.
- 13. The method of claim 12, wherein the inhibin cDNA has or contains substantially the same sequence as SEQ ID NO: 1.
- 14. The method of claim 12, wherein the inhibin protein, or fragment thereof, in the expressed fused heterologous protein has or contains substantially the same sequence as SEQ ID NO: 2.
- 15. The method of claim 12, wherein the carrier protein is maltose binding protein, bovine serum albumin, ovalbumin, flagellin, keyhole lympet hemocyanin, thyroglobulin, serum albumin, gamma globulin, syngeneic cells, syngeneic cells bearing Ia antigens, or polymers of amino acids.
- 16. A fusion gene product comprising a gene encoded for the expression of alpha-subunit avian inhibin protein, or a fragment thereof, fused to a gene encoded for the expression of a carrier protein.
- 17. The fusion gene product of claim 16, wherein the gene encoded for the expression of alpha-subunit avian inhibin protein is encoded for the expression of a protein having or containing substantially the same sequence as SEQ ID NO: 2.
- 18. The fusion gene product of claim 16, wherein the gene encoded for the expression of alpha-subunit avian inhibin protein is substantially the same sequence as SEQ ID NO: 1.
- 19. The fusion gene product of claim 16, wherein the gene encoded for the expression of a carrier protein is encoded for the production of maltose binding protein, bovine serum albumin, ovalbumin, flagellin, keyhole lympet hemocyanin, serum albumin, thyroglobulin, gamma globulin, syngeneic cells, syngeneic cells bearing Ia antigens, or polymers of amino acids.
- 20. A composition comprising a cell containing a vector, wherein the vector contains a DNA sequence encoding inhibin, or a fragment thereof, and a carrier protein and wherein the vector is capable of expressing inhibin, or a fragment thereof, fused to the carrier protein, when present in the cell.
- 21. The composition of claim 20 wherein the DNA sequence encoding inhibin, or a fragment thereof, is SEQ ID NO: 1.
- 22. A composition comprising a vector, wherein the vector contains a DNA sequence encoding inhibin, or a fragment thereof, and a carrier protein and wherein the vector is capable of expressing inhibin, or a fragment thereof, fused to the carrier protein, when present in the cell.
- 23. The composition of claim 22 wherein the DNA sequence encoding inhibin, or a fragment thereof, is SEQ ID NO: 1.
- 24. A method of enhancing production performance of a bird, comprising administering an effective amount of a fusion gene product comprising a gene encoded for the expression of alpha-subunit avian inhibin protein, or a fragment thereof, and a gene encoded for the expression of a carrier protein, to a bird such that the production performance of the bird is improved.
- 25. A method comprising, implanting into an animal a cell containing a vector, wherein the vector contains a DNA sequence encoding inhibin, or a fragment thereof, and a carrier protein, and wherein the vector is capable of expressing inhibin, or a fragment thereof, fused to the carrier protein, when present in the cell.
- 26. The method of claim 25 wherein the DNA sequence encoding inhibin, or a fragment thereof, is SEQ ID NO: 1.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This is a continuation-in-part application of U.S. patent application Ser. No. 08/395,554 filed Feb. 28, 1995, which is herein incorporated by reference, which is a continuation-in-part of U.S. patent application Ser. No. 08/202,964, filed Feb. 28, 1994, which is herein incorporated by reference.
Continuations (3)
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Number |
Date |
Country |
Parent |
09436805 |
Nov 1999 |
US |
Child |
10262581 |
Sep 2002 |
US |
Parent |
08984776 |
Dec 1997 |
US |
Child |
09436805 |
Nov 1999 |
US |
Parent |
08481633 |
Jun 1995 |
US |
Child |
08984776 |
Dec 1997 |
US |
Continuation in Parts (2)
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Number |
Date |
Country |
Parent |
08395554 |
Feb 1995 |
US |
Child |
08481633 |
Jun 1995 |
US |
Parent |
08202964 |
Feb 1994 |
US |
Child |
08395554 |
Feb 1995 |
US |