Claims
- 1. An affinity microcolumn comprising a high surface area material comprising glass, which has high flow properties and a low dead volume, contained within a housing and affinity reagents bound to the surface of the high surface area material, wherein the affinity reagents are either activated or activatable and the high surface area is formed by chemical etching of the surface.
- 2. The affinity microcolumn according to claim 1 wherein the affinity reagents that are bound to the surface of the high surface material further comprise affinity receptors bound to the affinity reagents.
- 3. The affinity microcolumn according to claim 2 further comprising a tethering molecule that is activated or activatable and binds the affinity receptors to the affinity reagents.
- 4. The affinity microcolumn according to claim 2 further comprising an amplification media interposed between the affinity reagents and the affinity receptors, where the amplification media allows better access by an analyte to the affinity receptors than in the absence of the amplification media.
- 5. The affinity microcolumn according to claim 4 wherein the amplification media comprises a biological polymer.
- 6. The affinity microcolumn of claim 1 wherein the housing is a micropipette.
- 7. The affinity microcolumn according to claim 1 further comprising an amplification media that is activated or activatible and is interposed between the affinity reagents and the affinity receptors, where the amplification media allows a high density of affinity receptors to be bound to the affinity reagents than in the absence of the amplification media.
- 8. The affinity microcolumn according to claim 7 wherein the amplification media comprises a biological polymer.
- 9. The affinity microcolumn according to claim 1 wherein the high surface area material comprises porous glass.
- 10. The affinity microcolumn according to claim 9 wherein the porous glass comprises a porous glass molecular trap that is formed by molding.
Parent Case Info
This application is a continuation-in-part of pending provisional applications Nos. 60/262,530 and 60/262,852, both filed on Jan. 18, 2001.
US Referenced Citations (12)
Provisional Applications (2)
|
Number |
Date |
Country |
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60/262530 |
Jan 2001 |
US |
|
60/262852 |
Jan 2001 |
US |