Methods and reagents for discovering and using mammalian melanocortin receptor agonists and antagonists to modulate feeding behavior in animals

Abstract
The present invention provides recombinant expression constructs comprising nucleic acid encoding mammalian melanocortin receptors, and mammalian cells into which said recombinant expression constructs have been introduced that express functional mammalian melanocortin receptors. The invention provides a panel of such transformed mammalian cells expressing melanocortin receptors for screening compounds for receptor agonist and antagonist activity. The invention also provides methods for using such panels of melanocortin receptor-expressing mammalian cells to specifically-detect and identify agonists and antagonists for each melanocortin receptor, as well as patterns of agonist and antagonist activity of said compounds for the class of melanocortin receptors. Such screening methods provide a means for identifying compounds with patterns of melanocortin agonist and antagonist activity which is associated with the capacity to influence or modify metabolism and behavior, particularly feeding behavior.
Description




BACKGROUND OF THE INVENTION




1. Field of the Invention




The present invention relates to the cloning, expression and functional characterization of mammalian melanocortin receptor genes. The invention provides nucleic acid encoding mammalian melanocortin receptors, recombinant expression constructs comprising said nucleic acid, and mammalian cells into which said recombinant expression constructs have been introduced, and that express functional mammalian melanocortin receptors. The invention also provides a panel of such transformed mammalian cells expressing melanocortin receptors for screening compounds for receptor agonist and antagonist activity. The invention provides methods, for using such panels of melanocortin receptor-expressing mammalian cells to specifically detect and identify agonists and antagonists for each melanocortin receptor, as well as patterns of agonist and antagonist activity of said compounds for the class of melanocortin receptors. Such screening methods provide a means for identifying compounds with patterns of melanocortin agonist and antagonist activity which is associated with the capacity to influence or modify physiological function and behavior, particularly metabolism and feeding behavior.




2. Background of the Invention




The proopiomelanocortin (POMC) gene product is processed to produce a large number of biologically active peptides. Two of these peptides, α-melanocyte stimulating hormone (αMSH), and adrenocorticotropic hormone (ACTH) have well understood roles in control of melanocyte and adrenocortical function, respectively. Both of these hormones are also found in a variety of forms with unknown functions, for example, γ-melanocyte stimulating hormone (γMSH), which has little or no ability to stimulate pigmentation (Ling et al., 1979


, Life Sci


. 25: 1773-1780; Slominski et al., 1992


, Life Sci


. 50: 1103-1108). A melanocortin receptor gene specific for each of the αMSH, ACTH and γMSH hormones has been discovered by some of the present inventors (see U.S. Pat. Nos. 5,280,112, 5,532,347 and U.S. application Ser. No. 08/044,812, incorporated by reference herein). In addition, two other melanocortin receptor genes have been discovered by some of the present inventors (see Lu et al, 1994


, Nature


371: 799-802; Mountjoy et al, 1994


, Molec. Endocrinol


. 8: 1298-1308) and others (see Gantz et al., 1993


, J. Biol. Chem


. 268: 15174-15179 and Labbe et al., 1994


, Biochem


. 33: 4543-4549).




Along with the well-recognized activities of αMSH in melanocytes and ACTH in adrenal and pituitary glands, the melanocortin peptides also have a diverse array of biological activities in other tissues, including the brain and immune system, and bind to specific receptors in these tissues with a distinct pharmacology (see, Hanneman et al., in


Peptide Hormone as Prohormones


, G. Martinez, ed. (Ellis Horwood Ltd.: Chichester, UK) pp. 53-82; DeWied & Jolles, 1982


, Physiol. Rev


. 62: 976-1059 for reviews). A complete understanding of these peptides and their diverse biological activities requires the isolation and characterization of their corresponding receptors. Some biochemical studies have been reported in the prior art.




Shimuze, 1985


, Yale J. Biol. Med


. 58: 561-570 discusses the physiology of melanocyte stimulating hormone.




Tatro & Reichlin, 1987


, Endocrinology


121: 1900-1907 disclose that MSH receptors are widely distributed in rodent tissues.




Sola et al., 1989


, J. Biol. Chem


. 264: 14277-14280 disclose the molecular weight characterization of mouse and human MSH receptors linked to radioactively and photoaffinity labeled MSH analogues.




Siegrist et al., 1991


, J. Receptor Res


. 11: 323-331 disclose the quantification of receptors on mouse melanoma tissue by receptor autoradiography.




Cone & Mountjoy, U.S. patent application Ser. No. 07/866,979, filed Apr. 10, 1992, disclose the isolation of human and mouse α-MSH receptor genes and uses thereof (incorporated herein by reference).




Cone & Mountjoy, U.S. patent application Ser. No. 07/866,560, filed Apr. 10, 1992, disclose the isolation of human and bovine ACTH receptor genes and uses thereof (incorporated herein by reference).




Mountjoy et al., 1992


, Science


257: 1248-1251 disclose the isolation of cDNAs encoding mammalian ACTH and MSH receptor proteins.




POMC neurons are present in only two regions of the brain, the arcuate nucleus of the hypothalamus, and the nucleus of the solitary tract of the brain stem. Neurons from both sites project to a number of hypothalamic nuclei known to be important in feeding behavior, including the paraventricular nucleus, lateral hypothalamic area, and ventromedial hypothalamic nucleus. While previous reports have claimed both stimulatory and inhibitory effects of α-MSH on feeding behavior (see Shimizu et al., 1989


, Life Sci


. 45: 543-552; Tsujii et al., 1989


, Brian Res. Bull


. 23: 165-169), knowledge of specific melanocortin receptors, their location within the central nervous system and the necessary pharmacological tools were not sufficiently developed at that time to allow the resolution of this issue. The present inventors have shown herein that a novel antagonist of the MC-3 and MC-4 melanocortin receptors can substantially increase food consumption in animals engaged in normal or fast-induced feeding behavior. This is consistent with expression of both MC-3 and MC-4 receptor mRNAs at these sites in in situ hybridization studies (Roselli-Rehfuss et al., 1993


, Proc. Natl. Acad. Sci. USA


90: 8856-8860; Mountjoy et al., 1994


, Molec. Endocrinol


. 8: 1298-1308). Moreover, the regulation of arcuate nucleus POMC gene expression is consistent with an inhibitory role for POMC in feeding behavior. POMC mRNA levels are decreased following a fast (Bergendahl et al., 1992


, Neuroendocrinol


. 56: 913-920; Brady et al., 1990


, Neuroendocrinol


. 52: 441-447), and a significant diurnal variation in POMC mRNA levels in the arcuate nucleus is seen in rat, with the nadir occurring around the onset of nighttime feeding at 1800 hrs (Steiner et al., 1994


, FASEB J


. 8: 479-488).




Thus, the experimental evidence indicates that POMC neurons play an important role in tonic inhibition of feeding behavior, wherein obesity results from a chronic disruption of this inhibitory tone by antagonism of central melanocortin receptors in at least one animal model (agouti).




These results reveal for the first time a need in the art for a means for characterizing mammalian melanocortin receptor agonists and antagonists in vitro for the development of compounds that affect feeding behavior in animals.




SUMMARY OF THE INVENTION




The present invention provides a biological screening system for identifying and characterizing compounds that are agonists or antagonists of mammalian melanocortin receptors. The biological screening system of the invention comprises a panel of transformed mammalian cells comprising a recombinant expression construct encoding a mammalian melanocortin receptor, and expressing said receptor thereby. The invention provides such a panel of transformed mammalian cells wherein the panel comprises cells expressing each of type of mammalian melanocortin receptor. Thus, the invention also provides nucleic acid encoding mammalian melanocortin receptors, recombinant expression constructs comprising said nucleic acid, and mammalian cells into which said recombinant expression constructs have been introduced, and that express functional mammalian melanocortin receptors. Methods for using such panels of melanocortin receptor-expressing mammalian cells to specifically detect and identify agonists and antagonists for each melanocortin receptor, as well as patterns of agonist and antagonist activity of said compounds for the class of melanocortin receptors, are also provided. Such screening methods provide a means for identifying compounds with patterns of melanocortin agonist and antagonist activity which is associated with the capacity to influence or modify metabolism and behavior in an animal, particularly feeding behavior.




Thus, the invention provides in a first aspect a biological screening panel for determining the melanocortin receptor agonist/antagonist profile of a test compound. The panel comprises a first mammalian cell comprising a recombinant expression construct encoding a mammalian melanocortin receptor that is the α-MSH (MC-1) receptor. The panel also comprises a second mammalian cell comprising a recombinant expression construct encoding a mammalian melanocortin receptor that is the ACTH (MC-2) receptor. The panel also comprises a third mammalian cell comprising a recombinant expression construct encoding a mammalian melanocortin receptor that is the MC-3 receptor. The panel also comprises a fourth mammalian cell comprising a recombinant expression construct encoding a mammalian melanocortin receptor that is the MC-4 receptor. The panel also comprises a fifth mammalian cell comprising a recombinant expression construct encoding a mammalian melanocortin receptor that is the MC-5 receptor. As provided by the invention, each mammalian cell expresses the melanocortin receptor encoded by the recombinant expression construct comprising said cell.




In preferred embodiments, the melanocortin receptors encoded by the recombinant expression constructs comprising the transformed mammalian cells comprising the panel are mouse MC-1 receptor (SEQ ID Nos.:3 and 4); human MC-I receptor (SEQ ID Nos.:5 and 6), human MC-2 (ACTH) receptor (SEQ ID Nos.:7 and 8), bovine MC-2 receptor (SEQ ID Nos.:9 and 10), rat MC-3 receptor (SEQ ID Nos.:11 and 12), human MC-4 receptor (SEQ ID Nos.:15 and 16) and human MC-5 receptor (SEQ ID Nos.:17 and 18).




In a second aspect, the invention provides a method for using the melanocortin receptor panel to identify and characterize test compounds as melanocortin receptor agonists and/or antagonists. In this embodiment, the method provided by the invention identifies a melanocortin receptor agonist, and comprises the steps of contacting each of the cells of the panel with a test compound to be characterized as an agonist of a mammalian melanocortin receptor and detecting binding of the test compound to each of the mammalian melanocortin receptors by assaying for a metabolite produced in the cells that bind the compound. In a preferred embodiment, the detected metabolite is cAMP.




In a preferred embodiment of this method, each of the cells of the panel of mammalian cells expressing mammalian melanocortin receptors further comprises a recombinant expression construct encoding a cyclic AMP responsive element (CRE) transcription factor binding site that is operatively linked to a nucleic acid sequence encoding a protein capable of producing a detectable metabolite. In preferred embodiments, said protein is β-galactosidase, most preferably encoded by a nucleic acid comprising the recombinant expression construct identified as pCRE/β-galactosidase (as disclosed in Chen et al., 1994


, Analyt. Biochem


. 226: 349-354). As provided by the invention, expression of the protein that produces the detectable metabolite is dependent on binding of the test compound to the melanocortin receptor expressed by each cell in the panel and the intracellular production of cAMP as a result. In this embodiment, cAMP production results in expression of a protein capable of producing a detectable metabolite, the protein most preferably being β-galactosidase. In preferred embodiments, the detectable metabolite absorbs light to produce a colored product. Thus, this embodiment of the invention provides a panel of melanocortin receptor-expressing cells whereby melanocortin hormone binding results in the production of a colored product in proportion to the extent of cAMP production in the cell as a result of hormone receptor binding.




In another embodiment of this aspect of the invention is provided a method for characterizing a compound as an antagonist of a mammalian melanocortin receptor. In this embodiment, the method comprises the steps of contacting each of the cells of the panel with an agonist of the mammalian melanocortin receptor in an amount sufficient to produce a detectable amount of a metabolite produced in the cells that bind the agonist, in the presence or absence of a test compound to be characterized as an antagonist of a mammalian melanocortin receptor, and detecting the amount of the metabolite produced in each cell in the panel in the presence of the test compound with the amount of the metabolite produced in each cell in the panel in the absence. As provided by the assay, inhibition of the production of the detectable metabolite is used as an indication that the tested compound is a melanocortin receptor antagonist, which is further characterized quantitatively by the extent of said inhibition.




In a preferred embodiment of this method, each of the cells of the panel of mammalian cells expressing mammalian melanocortin receptors further comprises a recombinant expression construct encoding a cyclic AMP responsive element (CRE) transcription factor binding site that is operatively linked to a nucleic acid sequence encoding a protein capable of producing a detectable metabolite. In preferred embodiments, said protein is β-galactosidase, most preferably encoded by a nucleic acid comprising the recombinant expression construct identified as pCRE/β-galactosidase. As provided by the invention, expression of the protein that produces the detectable, metabolite is dependent on binding of the test compound to the melanocortin receptor expressed by each cell in the panel. In preferred embodiments, the detectable metabolite absorbs light to produce a colored product. Thus, this embodiment of the invention provides a panel of melanocortin receptor-expressing cells whereby melanocortin hormone binding results in the production of a colored product in proportion to the extent of cAMP production in the cell as a result of hormone receptor binding.




The invention also provides melanocortin receptor agonists identified by the methods and using the screening panel of the invention. In preferred embodiments, the agonist is an agonist of the MC-3 mammalian melanocortin receptor. In preferred embodiments, the agonist is an agonist of the MC-4 mammalian melanocortin receptor.




The invention provides melanocortin receptor antagonists identified by the methods and using the screening panel of the invention. In preferred embodiments, the antagonist is an antagonist of the MC-3 mammalian melanocortin receptor. In preferred embodiments, the antagonist is an antagonist of the MC-4 mammalian melanocortin receptor.




The invention also provides methods for characterizing mammalian melanocortin receptor agonists for the capacity to modify or influence metabolism and feeding behavior in an animal. In a first aspect, the invention provides a method for characterizing melanocortin receptor MC-3 or MC-4 agonists as inhibitors of feeding behavior in an animal, the method comprising the steps of providing food to an animal that has been deprived of food for at least 12 hours, with or without administering to the animal an MC-3 or MC-4 receptor agonist of the invention, and comparing the amount of food eaten by the animal after administration of the MC-3 or MC-4 receptor agonist with the amount of food eaten by the animal without administration of the MC-3 or MC-4 receptor agonist.




In another aspect, the invention provides a method for characterizing a melanocortin MC-3 or MC-4 receptor antagonist as a stimulator of feeding behavior in an animal. In this embodiment, the method comprises the steps of providing food to an animal not deprived of food for at least 12 hours, with or without administering to the animal an MC-3 or MC-4 receptor antagonist, immediately prior to the onset of darkness or nighttime, and comparing the amount of food eaten by the animal after administration of the MC-3 or MC-4 receptor antagonist with the amount of food eaten by the animal without administration of the MC-3 or MC-4 receptor antagonist.




Thus, the invention also provides methods for using certain of the melanocortin receptor agonists and antagonists for modifying feeding behavior in an animal. In a first aspect, the invention provides a method for stimulating feeding in an animal, the method comprising administering to the animal an MC-3 or MC-4 receptor antagonist. In a preferred embodiment, the antagonists are administered systemically. In additional embodiments, the antagonists are administered intracerebroventricularly.




In another aspect, the invention provides a method for inhibiting feeding in an animal, the method comprising administering to the animal an MC-3 or MC-4 receptor agonist. In a preferred embodiment, the agonists are administered systemically. In additional embodiments, the agonists are administered intracerebroventricularly. In yet another aspect, the invention provides mammalian melanocortin receptor agonists having the general formula:






A-B-C-D-E-F-G-amide






wherein A is an aliphatic amino acid residue, including for example Leu, Ile, Nle and Met, as well as analogues and substituted derivatives thereof; B is an acidic amino acid residue, including for example Asp and Glu; C is a basic amino acid residue, such as His; D is an aromatic amino acid residue having a


D


-conformation, including


D


-Phe,


D


-Tyr and substituted derivatives thereof; E is. a basic amino acid residue, for. example Arg, Lys, homoArg, homoLys, and analogues or substituted derivatives thereof; F is Trp or substituted derivatives thereof; and G is Lys, homoLys or a substituted derivative thereof. In the peptide embodiments of the melanocortin receptor agonists of the invention, the peptide is cyclized by the formation of an amide bond between the side chain carboxyl group of the Asp or Glu residue at position B in the peptide, and the side chain amino group of the Lys or homoLys residue at position G. In preferred embodiments, the melanocortin receptor agonists of the invention are agonists of the MC-3 or MC-4 receptor.




The invention also provides mammalian melanocortin receptor antagonists having the general formula:






A-B-C-D-E-F-G-amide






wherein A is an aliphatic amino acid residue, including for example Leu, Ile, Nle and Met, as well as analogues and substituted derivatives thereof; B is an acidic amino acid residue, including for example Asp and Glu; C is a basic amino acid residue, such as His; D is an aromatic amino acid residue having


D


-conformation, including


D


-Nal and substituted derivatives thereof; E is a basic amino acid residue, for example Arg, Lys, homoArg, homoLys, and analogues or substituted derivatives thereof; F is Trp or substituted derivatives thereof; and G is Lys, homoLys or a substituted derivative thereof. In the peptide embodiments of the melanocortin receptor antagonists of the invention, the peptide is cyclized by the formation of an amide bond between the side chain carboxyl group of the Asp or Glu residue at position B in the peptide, and the side chain amino group of the Lys or homoLys residue at position G. In preferred embodiments, the melanocortin receptor antagonists of the invention are agonists of the MC-3 or MC-4 receptor.




It is an advantage of the present invention that it provides an in vitro screening method for characterizing compounds having melanocortin receptor activities that relate to feeding behavior in animals. Specifically, the invention advantageously provides means and methods for identifying compounds having melanocortin receptor agonist and/or antagonist activity that have been associated with either stimulating or inhibiting feeding behavior when administered to an animal. The invention thus provides an economical first step in screening compounds for the capacity to affect feeding behavior, including synthetic, peptidomimetic or organomimetic derivatives of melanocortin receptor agonists or antagonists as disclosed herein or elsewhere.




Specific preferred embodiments of the present invention will become evident from the following more detailed description of certain preferred embodiments and the claims.











DESCRIPTION OF THE DRAWINGS





FIGS. 1A and 1B

illustrate the nucleotide (SEQ ID No.:3) and amino acid (SEQ ID No.:4) sequence of the mouse melanocyte stimulating hormone receptor gene.





FIGS. 2A and 2B

illustrate the nucleotide (SEQ ID No.:5) and amino acid (SEQ ID No.:6) sequence of the human melanocyte stimulating hormone receptor gene.





FIGS. 3A through 3C

illustrate the nucleotide (SEQ ID No.:7) and amino acid (SEQ ID No.:8) sequence of the human adrenocorticotropic hormone receptor gene.





FIGS. 4A and 4B

illustrate the nucleotide (SEQ ID No.:9) and amino acid (SEQ ID No.:10) sequence of the bovine adrenocorticotropic hormone receptor gene.





FIGS. 5A and 5B

illustrate the nucleotide (SEQ ID No.:11) and amino acid (SEQ ID No.:12) sequences of the rat melanocortin-3 receptor.





FIGS. 6A through 6C

illustrate the nucleotide (SEQ ID No.:15) and amino acid (SEQ ID No.:16) sequence of the human melanocortin 4 receptor gene.





FIGS. 7A and 7B

illustrate the nucleotide (SEQ ID No.:17) and amino acid (SEQ ID No.:18) sequences of the rat melanocortin-5 receptor gene.





FIG. 8

shows a graph of intracellular cAMP accumulation resulting from melanocyte stimulating hormone receptor agonist binding in human 293 cells transfected with a MSH receptor-encoding recombinant expression construct.





FIG. 9

illustrates the cAMP response of mouse Y1 cells to binding of melanocortin peptides to human melanocortin-2 (ACTH) receptor, as measured using the β-galactosidase assay described in Example 4.





FIG. 10

illustrates the results of competition binding experiments of melanocortin pepfides to cells expressing a recombinant expression construct encoding the rat melanocortin-3 receptor.





FIGS. 11A through 11C

illustrate the results of experiment showing intracellular cAMP accumulation caused by receptor-ligand binding in human 293 cells expressing the MC-3 receptor.





FIG. 12

shows a graph of intracellular cAMP accumulation resulting from peptides to human melanocortin-4 receptor agonist binding in human 293 cells transfected with a MC-4 receptor-encoding recombinant expression construct.





FIG. 13

illustrates the results of cAMP accumulation (AC) and cAMP-dependent β-galactosidase (β-gal) assays of melanocortin peptide binding to a rat melanocortin-5 receptor.





FIG. 14

illustrates the structure of the pCRE/β-gal plasmid.





FIG. 15

illustrates the results of the β-galactosidase-coupled, colorimetric melanocortin receptor binding assay using cells expressing each of the MC-1, MC-3, MC-4 or MC-5 receptors and contacted with αMSH or a variety of αMSH analogues.





FIG. 16

shows the results of the β-galactosidase-coupled, colorimetric melanocortin receptor binding assay to determine antagonist activity of melanocortin analogues SHU9119 and SHU8914 in cells expressing each of the melanocortin receptors MC-1, MC-3, MC-4 and MC-5.





FIG. 17

shows the results of classic competition binding assays using the melanocortin analogues SHU9119 and SHU8914 at the MC3-R and MC-4 R receptors.





FIGS. 18A and 18B

shows the results of cAMP accumulation experiments (performed using the β-galactosidase assay of Example 4) for mouse MC-4 receptor (

FIG. 18A

) and rat MC-5 receptor (FIG.


18


B).





FIGS. 19A through 19C

show the effect on food intake of intracerebroventricular administration of melanocortin analogue SHU9119 in mice.





FIGS. 20A through 20C

show the effect on food intake of intracerebroventricular administration of melanocortin analogue MTII in mice.





FIG. 20D

shows the effect on locomotor activity of intracerebroventricular administration of melanocortin analogue MTII in mice.





FIGS. 21A through 21D

show the effect on food intake of intracerebroventricular administration of melanocortin analogue MTII in mice.











DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS




The term “melanocortin receptor” as used herein reference to proteins having the biological activity of any of the disclosed melanocortin receptors, including the MC-1 (SEQ ID Nos.:3, 4, 5 and 6), MC-2 (ACTH; SEQ ID Nos.:7, 8, 9 and 10), MC-3 (SEQ ID Nos.:11 and 12), MC-4 (SEQ ID Nos.:15 and 16) or MC-5 (SEQ ID Nos.:17 and 18) receptors, as well as naturally-occurring and genetically-engineered allelic variations in these sequences.




Cloned nucleic acid provided by the present invention may encode MC receptor protein of any species of origin, including, for example, mouse, rat, rabbit, cat, and human, but preferably the nucleic acid provided by the invention encodes MC receptors of mammalian, most preferably rodent and human, origin.




The production of proteins such as the MC receptors from cloned genes by genetic engineering means is well known in this art. The discussion which follows is accordingly intended as an overview of this field, and is not intended to reflect the full state of the art.




DNA which encodes MC receptors may be obtained, in view of the instant disclosure, by chemical synthesis, by screening reverse transcripts of mRNA from appropriate cells or cell line cultures, by screening genomic libraries from appropriate cells, or by combinations of these procedures, as illustrated below. Screening of mRNA or genomic DNA may be carried out with oligonucleotide probes generated from the MC receptor gene sequence information provided herein. Probes may be labeled with a detectable group such as a fluorescent group, a radioactive atom or a chemiluminescent group in accordance with know procedures and used in conventional hybridization assays, as described in greater detail in the Examples below. In the alternative, MC receptor gene sequences may be obtained by use of the polymerase chain reaction (PCR) procedure, with the PCR oligonucleotide primers being produced from the MC receptor gene sequences provided herein. See U.S. Pat. No. 4,683,195 to Mullis et al. and U.S. Pat. No. 4,683,202 to Mullis.




MC receptor proteins may be synthesized in host cells transformed with a recombinant expression construct comprising a nucleic acid encoding each of the receptors disclosed herein. Such a recombinant expression construct can also be comprised of a vector that is a replicable DNA construct. Vectors are used herein either to amplify DNA encoding an MC receptor and/or to express DNA which encodes an MC receptor. For the purposes of this invention, a recombinant expression construct is a replicable DNA construct in which a DNA sequence encoding an MC receptor is operably linked to suitable control sequences capable of effecting the expression of the receptor in a suitable host cell. The need for such control sequences will vary depending upon the host selected and the transformation method chosen. Generally, control sequences include a transcriptional promoter, an optional operator sequence to control transcription, a sequence encoding suitable mRNA ribosomal binding sites, and sequences which control the termination of transcription and translation. Amplification vectors do not require expression control domains. All that is needed is the ability to replicate in a host, usually conferred by an origin of replication, and a selection gene to facilitate recognition of transformants. See, Sambrook et al., 1990


, Molecular Cloning: A Laboratory Manual


(Cold Spring Harbor Press: New York).




Also specifically provided by the invention are reporter expression constructs comprising a nucleic acid encoding a protein capable of expressing a detectable phenotype, such as the production of a detectable reporter molecule, in a cell expressing the construct. Such constructs can be used for producing recombinant mammalian cell lines in which the reporter construct is stably expressed.




Most preferably, however, the reporter construct is provided and used to induce transient expression over an experimental period of from about 18 to 96 hrs in which detection of the reporter protein-produced detectable metabolite comprises an assay. Such reporter expression constructs are also provided wherein induction of expression of the reporter construct is controlled by a responsive element operatively linked to the coding sequence of the reporter protein, so that expression is induced only upon proper stimulation of the responsive element. Exemplary of such a responsive element is a cAMP responsive element (CRE), which induces expression of the reporter protein as a result of an increase in intracellular cAMP concentration. In the context of the present invention, such a stimulus is associated with melanocortin receptor binding, so that a reporter construct comprising one or more CREs is induced to express the reporter protein upon binding of a receptor agonist to a MC receptor in a recombinantly transformed mammalian cell. Production and use of such a reporter construct is illustrated below in Example 5.




Vectors useful for practicing the present invention include plasmids, viruses (including phage), retroviruses, and integratable DNA fragments (i.e., fragments integratable into the host genome by homologous recombination). The vector replicates and functions independently of the host genome, or may, in some instances, integrate into the genome itself. Suitable vectors will contain replicon and control sequences which are derived from species compatible with the intended expression host. A preferred vector is the plasmid pcDNA/neo I. Transformed host cells are cells which have been transformed or transfected with recombinant expression constructs made using recombinant DNA techniques and comprising mammalian MC receptor-encoding sequences. Preferred host cells are human 293 cells. Preferred host cells for the MC-2 (ACTH) receptor are Y1 cells (subclone OS3 or Y6). Transformed host cells are chosen that ordinarily express functional MC receptor protein introduced using the recombinant expression construct. When expressed, the mammalian MC receptor protein will typically be located in the host cell membrane. See, Sambrook et al., ibid.




Cultures of cells derived from multicellular organisms are a desirable host for recombinant MC receptor protein synthesis. In principal, any higher eukaryotic cell culture is workable, whether from vertebrate or invertebrate culture. However, mammalian cells are preferred, as illustrated in the Examples. Propagation of such cells in cell culture has become a routine procedure. See


Tissue Culture


, Academic Press, Kruse & Patterson, editors (1973). Examples of useful host cell lines are human 293 cells, VERO and HeLa cells, Chinese hamster ovary (CHO) cell lines, mouse Y1 (subclone OS3), and WI138, BHK, COS-7, CV, and MDCK cell lines. Human 293 cells are preferred.




Cells expressing mammalian MC receptor proteins made from cloned genes in accordance with the present invention may be used for screening agonist and antagonist compounds for MC receptor activity. Competitive binding assays are well known in the art and are described in the Examples below. Such assays are useful for drug screening of MC receptor agonist and antagonist compounds, as detected in receptor binding assays as described below.




One particular use of such screening assays are for developing drugs and other compounds useful in modifying or changing feeding behavior in mammals. The invention provides an assay system, comprising a panel of recombinant mammalian cells, heterologously expressing each of the MC receptors disclosed herein, wherein the panel is constructed of at least one cell line expressing an MC receptor. The invention provides such panels also comprising a detection means for detecting receptor agonist or antagonist binding, such as the reporter expression constructs described herein, using direct binding and competition binding assays as described in the Examples below. In the use of this panel, each MC receptor is assayed for agonist or antagonist patterns of binding a test compound, and a characteristic pattern of binding for all MC receptors is thereby determined for each test compound. This pattern is then compared with known MC receptor agonists and antagonists to identify new compounds having a pattern of receptor binding activity associated with a particular behavioral or physiological effect.




For example, provided herein is experimental evidence that MC-3 or MC-4 receptor antagonists are capable of stimulating feeding in hungry animals, and that MC-3 or MC-4 agonists are capable of inhibiting feeding in animals otherwise stimulated to eat. The invention provides an in vitro assay to characterize MC-3 and MC-4 agonists/antagonists as a preliminary and economical step towards developing feeding behavior-modulating drugs for use in vivo.




These results on feeding behavior in vivo have been obtained with certain MC receptor binding analogues, SHU9119 and MTII. These compounds have the following chemical structure:











(An additional analogue, SHU8914, has been tested in in vitro receptor binding assays; this compound is identical in structure to SHU9119, except that the amino acid analogue at position 7 is para-iodo-D-phenylalanine.)




Generally, those skilled in the art will recognize that peptides as described herein may be modified by a variety of chemical techniques to produce compounds having essentially the same activity as the unmodified peptide, and optionally having other desirable properties. For example, carboxylic acid groups of the peptide, whether carboxyl-terminal or sidechain, may be provided in the form of a salt of a pharmaceutically-acceptable cation or esterified to form a C


1


-C


16


ester, or converted to an amide of formula NR


1


R


2


wherein R


1


and R


2


are each independently H or C


1


-C


16


alkyl, or combined to form a heterocyclic ring, such as 5- or 6-membered. Amino groups of the peptide, whether aminoterniinal or sidechain, may be in the form of a phannaceutically-acceptable acid addition salt, such as the HCl, HBr, acetic, benzoic, toluene sulfonic, maleic, tartaric and other organic salts, or may be modified to C


1


-C


16


alkyl or dialkyl amino or further converted. to an amide. Hydroxyl groups of the peptide side chain may be converted to C


1


-C


16


alkoxy or to a C


1


-C


16


ester using well-recognized techniques. Phenyl and phenolic rings of the peptide sidechain may be substituted with one or more halogen atoms, such as fluorine, chlorine, bromine or iodine, or with C


1


-C


16


alkyl, C


1


-C


16


alkoxy, carboxylic acids and esters thereof, or amides of such carboxylic acids. Methylene groups of the peptide sidechains can be extended to homologous C


2


-C


4


alkylenes. Thiols can be protected with any one of a number of well-recognized protecting groups, such as acetamide groups. Those skilled in the art will also recognize methods for introducing cyclic structures into the peptides of this invention to select and provide conformational constraints to the structure that result in enhanced binding and/or stability. For example, a carboxyl-terminal or amino-terminal cysteine residue can be added to the peptide, so that when oxidized the peptide will contain a disulfide bond, thereby generating a cyclic peptide. Other peptide cyclizing methods include the formation of thioethers and carboxyl- and amino-terminal amides and esters.




Peptidomimetic and organomimetic embodiments are also hereby explicitly declared to be within the scope of the present invention, whereby the three-dimensional arrangement of the chemical constituents of such peptido- and organonimetics mimic the three-dimensional arrangement of the peptide backbone and component amino acid sidechains in the peptide, resulting in such peptido- and organomimetics of the peptides of this invention having substantial biological activity. It is implied that a pharmacophore exists for the receptor agonist and antagonist properties of these and related MC receptor binding analogues. A pharmacophore is an idealized, three-dimensional definition of the structural requirements for biological activity. Peptido- and organomimetics can be designed to fit each pharmacophore with current computer modeling software (computer aided drug design). MC receptor binding analogues derived using such software and comprising peptido- and organomimetics of SHU9119 and MTII and related analogues are within the scope of the claimed invention.




The MC receptor binding analogues, in particular those analogues that are MC-3 or MC-4 receptor agonists or antagonists are provided to be used in methods of influencing, modifying or changing feeding behavior in mammals in vivo. Specific examples of uses for the MC receptor binding analogues of the invention include but are not limited to treatment of eating disorders such as anorexia and obesity, and other pathological weight and eating-related disorders. Other examples are failure to thrive disorders and disease-related cachexia, such as occurs in cancer patients. Also within the scope of the analogues of the invention is use for enhancing appearance, athletic ability, or adjuvant to other therapies to treat disorders such as high blood pressure, high serum cholesterol, vascular and heart disease, stroke, kidney disease, diabetes and other metabolic disorders.




The Examples which follow are illustrative of specific embodiments of the invention, and various uses thereof. They set forth for explanatory purposes only, and are not to be taken as limiting the invention.




EXAMPLE 1




Isolation of an αMSH Receptor Probe by Random PCR Amplification of Human Melanoma cDNA Using Degenerate Oligonucleotide Primers




In order to clone novel G-protein coupled receptors, cDNA prepared from RNA from human melanoma cells was used as template for a polymerase chain reaction (PCR)-based random cloning experiment. PCR was performed using a pair of degenerate oligonucleotide primers corresponding to the putative third and sixth transmembrane regions of G-protein coupled receptors (Libert et al., 1989


, Science


244: 569-72; Zhou et al., 1990


, Nature


347: 76-80). The PCR products obtained in this experiment were characterized by nucleotide sequencing. Two novel sequences representing novel G-protein-coupled receptors were identified.




PCR amplification was performed as follows. Total RNA was isolated from a human melanoma tumor sample by the guanidinium thiocyanate method (Chirgwin et al., 1979


, Biochemistry


18: 5294-5299). Double-stranded cDNA was synthesized from total RNA with murine reverse transcriptase (BRL, Gaithersburg, Md.) by oligo-dT priming (Sambrook et al., ibid.). The melanoma cDNA mixture was then subjected to 45 cycles of PCR amplification using 500 picomoles of degenerate oligonucleotide primers having the following sequence:




Primer III (sense):




GAGTCGACCTGTG(CIT)G(C/T)(C/G)AT(C/T)(A/G)CIIT(G/T)GAC(C/A)G(C/G)TAC (SEQ ID NO:1)




and




Primer VI (antisense):




CAGAATTCAG(T/A)AGGGCAICCAGCAGAI(G/C)(G/A)(T/C)GAA (SEQ ID NO:2)




in 100 μl of a solution containing 50 mM Tris-HCl (pH 8.3), 2.5 MM MgCl


2


, 0.01% gelatin, 200 μM each DNTP, and 2.5 units of Taq polymerase (Saiki et al., 1988


, Science


239: 487-491). These primers were commercially synthesized by Research Genetics Inc. (Huntsville, Ala.). Each PCR amplification cycle consisted of incubations at 94° C. for 1 min (denaturation), 45° C. for 2 min (annealing), and 72° C. for 2 min (extension).




Amplified products of the PCR reaction were extracted with phenol/chloroform and precipitated with ethanol. After digestion with EcoRI and SalI, the PCR products were separated on a 1.2% agarose gel. A slice of this gel, corresponding to PCR products of 300 basepairs (bp) in size, was cut out and purified using glass beads and sodium iodide, and the insert was then cloned into a pBKS cloning vector (Stratagene, LaJolla, Calif.).




A total of 172 of such pBKS clones containing inserts were sequenced using Sequenase (U.S. Biochemical Corp., Cleveland, Ohio) by the dideoxynucleotide chain termination method (Sanger et al., 1977


, Proc. Natl. Acad Sci. USA


74: 5463-5467). Two types of sequences homologous to other G-protein coupled receptors were identified.




EXAMPLE 2A




Isolation of a Mouse αMSH (MC-1) Receptor cDNA




Probes isolated in Example 1 was used to screen a Cloudman melanoma cDNA library in order to isolate a full-length cDNA corresponding to the cloned probe. One clone was isolated from a library of 5×10


6


clones screened as described below. This clone contained an insert of 2.6 kilobases (kb). The nucleotide sequence of the complete coding region was determined (see co-owned U.S. Pat. No. 5,532,347, incorporated by reference); a portion of this cDNA comprising the coding region was sequenced and is shown in

FIGS. 1A and 1B

(SEQ ID Nos:3 & 4).




EXAMPLE 2B




Isolation of a Human αMSH (MC-1) Receptor cDNA




In order to isolate a human counterpart of the murine melanocyte αMSH receptor gene disclosed in Example 2A and co-owned U.S. Pat. No. 5,532,347, a human genomic library was screened at high stringency (50% formamide, 42° C.) using the human PCR fragments isolated as described in Example 1. A genomic clone was determined to encode an human MSH receptor (SEQ ID NO:5.; FIGS.


2


A and


2


B). The human MSH receptor has a predicted amino acid sequence (SEQ ID NO:6) that is 75% identical and colinear with the mouse αMSH receptor cDNA sequence. The predicted molecular weight of the human MSH


R


is 34.7 kD.




EXAMPLE 2C




Isolation of a Human ACTH (MC-2) Receptor cDNA




For cloning the ACTH receptor (MC-2), a human genomic library was screened at high stringency (50% formamide, 1M NaCl, 50 nM Tris-HCl, pH 7.5, 0.1% sodium pyrophosphate, 0.2% sodium dodecyl sulfate, 100 μg/ml salmon sperm DNA, 10×Denhardt's solution, 42° C.), using the human PCR fragments isolated as described in Example 1 herein and U.S. Pat. No. 5,280,112, incorporated by reference. A genomic clone was isolated that encodes a highly related G-coupled receptor protein (SEQ ID No:7 and FIGS.


3


A and


3


B). The predicted amino acid sequence (SEQ ID NO:8) of this clone is 39% identical and also colinear, excluding the third intracellular loop and carboxy-terminal tail, with the human MSH receptor gene product. The predicted molecular weight of this putative ACTHR is 33.9 kilodaltons (kD). This clone was identified as encoding an MC-2 receptor based on its high degree of homology to the murine and human MSH receptors, and the pattern of expression in different tissue types, as described in Example 3 in U.S. Pat. No. 5,280,112, incorporated by reference herein.




EXAMPLE 2D




Isolation of a Bovine ACTH (MC-2) Receptor cDNA




A bovine genomic DNA clone encoding the bovine counterpart of the MC-2 (ACTH) receptor was isolated from a bovine genomic library, essentially as described in Example 2C above, and its nucleotide sequence determined (as shown in

FIGS. 4A and 4B

; SEQ ID Nos:9 & 10).




EXAMPLE 2E




Isolation of a Rat γ-MSH (MC-3) Receptor cDNA




The mouse αMSH receptor cDNA isolated as described in Example 2A and co-owned U.S. Pat. No. 5,532,347 was used to screen a rat hypothalamus cDNA library at low stringency (30% formnamide, 5×SSC, 0.1% sodium pyrophosphate, 0.2% sodium dodecyl sulfate, 100 μg/ml salmon sperm DNA, and 10% Denhardt's solution) at 42° C. for 18 h. A 1 kb cDNA clone was isolated and sequenced as described in co-owned U.S. Pat. No. 5,532,347, and this clone used to re-screen the rat hypothalamus cDNA library at high stringency (same conditions as above except that formamide was present at 45%). A cDNA clone approximately 2.0 kb in length was isolated and analyzed as described in co-pending U.S. application Ser. No. 08/044,812, incorporated by reference; a portion of this cDNA comprising the coding region was sequenced and is shown in

FIGS. 5A and 5B

(SEQ ID Nos:11 & 12).




EXAMPLE 2F




Isolation of a Human MC-4 Receptor DNA




For cloning the MC-4 receptor, a human genomic library was screened at moderate stringency (40% formamide, 1M NaCl, 50 mM Tris-HCl, pH 7.5, 0.1% sodium pyrophosphate, 0.2% sodium dodecyl sulfate, 100 μg/ml salmon sperm DNA, 10×Denhardt's solution, 42° C.), using rat PCR fragments isolated as described in Example 1 herein, with the exception that the following primers were used for PCR:




Primer II (sense):




GAGTCGACC(A/G)CCCATGTA(C/T)T(AGT)(C/T)TTCATCTG (SEQ ID NO:13)




and




Primer VII (antisense):




CAGAATTCGGAA(A/G)GC(A/G)TA(G/T)ATGA(A/G)GGGGTC (SEQ ID NO:14)




A genomic clone was isolated that encodes a highly related G-coupled receptor protein (SEQ ID NO:15 and

FIGS. 6A and 6B

) on a 1.9 kb HindIII fragment. The predicted amino acid sequence (SEQ ID NO:16) of this clone shares 55-61% sequence identity with human MC-3 and MC-5 receptors, and 46-47% sequence identity with the human MC-1 and MC-2 (ACTH) receptor.




EXAMPLE 2G




Isolation of a Mouse MC-5 Receptor DNA




One million clones from a mouse 129SVJ genomic library comprising 5,000,000 clones in the λFixII vector (Stratagene) were screened at low stringency (hybridization in 40% formamide at 42° C., washing performed in 0.5×SSC at 60° C., as described above in Example 2E) using radiolabeled probes from the rat MC-3 and MC-4 receptors, as described in Examples 2E and 2F). Positively-hybridizing clones were isolated and sequenced, and the sequences obtained were compared to previously-isolated melanocortin receptor clones. One clone, comprising a previously-unknown sequence, was determined to encode the MC-5 melanocortin receptor. The nucleotide and amino acid sequences of this receptor are shown in

FIGS. 7A and 7B

(SEQ ID No.:17 & 18).




EXAMPLE 3




Construction of a Recombinant Expression Construct, DNA Transfection and Functional Expression of the MCR Gene Products




In order to produce recombinant mammalian cells expressing each of the melanocortin receptors of Example 2, cDNA or the coding exons from genomic. DNA from each receptor was cloned into a mammalian expression construct, the resulting recombinant expression construct transfected into human 293 cells, and cell lines generated that expressed the melanocortin receptor proteins in cellular membranes at the cell surface.




The mouse αMSH receptor was cloned by excising the entire coding region of the αMSH


R


(MC-1) cDNA insert comprising a 2.1 kb fragment and subcloning this fragment into the BamHI/XhoI sites of pcDNAI/neo expression vector (Invitrogen, San Diego, Calif.). The resulting plasmid was prepared in large-scale through one cycle of CsCl gradient ultracentrifugation, and 20 μg of the plasmid transfected into each 100 mm dish of 293 cells using the calcium phosphate method (see Chen & Okayama, 1987


, Mol. Cell. Biol


. 7: 2745-2752). After transfection, cells were cultured in DMEM media supplemented with 10% calf serum in a 3% CO


2


atmosphere at 37° C. Selection was performed with neomycin (G418; GIBCO) at a concentration of 1000 μg/mL; selection was started 72 hr after transfection and continued for 3 weeks.




The αMSH


R


is known to couple to G-proteins and thereby activate adenyl cyclase, increasing intracellular levels of cAMP (see Buckley & Ramachandran, 1981


, Proc. Natl. Acad. Sci. USA


78: 7431-7435; Grahame-Smith et al., 1967


, J. Biol. Chem


242: 5535-5541; Mertz & Catt, 1991


, Proc. Natl. Acad. Sci. USA


88: 8525-8529; Pawalek et al., 1976, Invest. Dermatol. 66: 200-209). This property of cells expressing the αMSH receptor was used analyze expression of the αMSH receptor in cell colonies transfected with the expression vectors described herein as follows. Cells (˜1×10


6


) were plated in 6-well dishes, washed once with DMEM containing 1% bovine serum albumin (BSA) and 0.5 mM IBMX (a phosphodiesterase inhibitor), then incubated for 45 minutes at 37° C. with varying concentrations of the melanotropic peptides αMSH, βMSH, γMSH, the MSH peptide analogues Nle


4


, D-Phe


7


-αMSH (NDP-MSH), and ACTH. Following hormone treatment, the cells were washed twice with phosphate buffered saline and intracellular cAMP extracted by lysing the cells with 1 mL of 60% ethanol. Intracellular cAMP concentrations were determined using an assay (Amersham) which measures the ability of cAMP to displace [8-


3


H] cAMP from a high affinity cAMP binding protein (see Gilman, 1970


, Proc. Natl. Acad. Sci. USA


67: 305-312).




The results of these experiments are shown in FIG.


8


. The abscissa indicates the concentration of each hormone and the ordinate indicates the percentage of basal intracellular cAMP concentration achieved by each treatment. Points indicate the mean of duplicate incubations; the standard error did not exceed 15% for any data point. None of the peptides tested induced any change in intracellular cAMP in cells containing the vector alone. Cells expressing the murine αMSH receptor responded to melanotropic peptides with a 2-3 fold elevation of intracellular cAMP, similar to levels of cAMP induced by these peptides in the Cloudman cell line (see Pawalek, 1985


, Yale J. Biol. Med


. 58: 571-578). The EC


50


values determined for αMSH (2.0×10


−9


M), ACTH (8.0×10


−9


M) and the superpotent MSH analogue NDP-MSH (2.8×10


−11


M) correspond closely to reported values (see Tatro et al., 1990


, Cancer Res


. 50: 1237-1242). As expected, the βMSH peptide had an EC


50


value comparable to αMSH, while γMSH had little or no activity (see Slominski et al., 1992


, Life Sci


. 50: 1103-1108), confirming the identity of this receptor as a melanocyte αMSH receptor.




A similar series of experiments were performed using mouse Y1 cells (subclone OS3; Schimmer et al., 1995


, J. Cell. Physiol


. 163: 164-171) expressing the human and bovine MC-2 (ACTH) receptor clones of Examples 2C and 2D. These results are shown in

FIG. 9

, where the extent of cAMP responsive element-linked β-galactosidase activity (see Example 4, below) is shown with increasing concentrations of ACTH.




The entire coding region of the MC-3 receptor cDNA insert, obtained as described above in the co-pending U.S. Ser. No. 08/044,812, was contained in a 2.0 kb restriction enzyme digestion fragment and was cloned into the BamHI/IXhoI sites of pcDNA/neo I expression vector (Invitrogen, San Diego, Calif.). The resulting plasmid was prepared in large-scale through one cycle of CsCl gradient ultracentrifugation and 20 μg pcDNA/MC-3 receptor DNA were transfected into each 100 mm dish of 293 cells using the calcium phosphate method (see Chen & Okayama, 1987


, Mol. Cell. Biol


. 7: 2745-2752). After transfection, cells were cultured in DMEM media supplemented with 10% calf serum in a 3% CO


2


atmosphere at 37° C. Selection was performed with neomycin (G418; GIBCO) at a concentration of 1000 μg/mL; selection was started 72 h after transfection and continued for 3 weeks.




Specific binding of melanocortin peptides to cells expressing the MC-3 receptor was demonstrated by competition experiments using


125


I-labeled Nle


4


-D-Phe


7


-α-MSH (NDP-MSH, as described in Tatro et al., 1990


, Cancer Res


. 50: 1237-1242). Suspended cells (2×10


5


) were incubated at 37° C. with 500,000 cpm of labeled peptide for 10 min in binding buffer (Ham's F10 media plus 10 mM HEPES, pH 7.2, 0.25% bovine serum albumin, 500K IU/mL aprotinin, 100 μg/mL bacitracin and 1 mM 1,10-phenanthroline) in the presence or absence of the indicated concentrations of peptides. Maximum labeling was achieved within 10 min.




The results of these experiments are shown in FIG.


10


. Labeled NDP-MSH binding to cells expressing the MC-3 receptor, produced as described above, is inhibited by competition with unlabeled peptides known to be melanocortin receptor agonists, having a relative order of potency as follows:




NDP-MSH>γ-MSH>α-MSH>ACTH


4-10


>>>ORG2766.




Approximate K


1


values derived from this experiment are as shown in Table I:















TABLE I











Agonist




K


i


(approx)













NDP-MSH




2 × 10


−8









γ-MSH




5 × 10


−8









α-MSH




1 × 10


−7









ACTH


4-10






8 × 10


−5

















cAMP production assays as described above were also used to analyze expression of MC3-R in cells transfected with the expression vectors described herein as follows. Cells (˜5×10


6


) were plated in 6-well dishes, washed once with DMEM containing 1% bovine serum albumin (BSA) and 0.5 mM IBMX (a phosphodiesterase inhibitor), then incubated for 1 h at 37° C. with varying concentrations of the melanotropic peptides αMSH, γ


3


MSH, γMSH, the MSH peptide analogues Nle


4


-D-Phe


7


-αMSH (NDP-MSH), ACTH


4-10


and ACTH


1-39


. Following hormone treatment, the cells were washed twice with phosphate buffered saline and intracellular cAMP extracted by lysing the cells with 1 mL of 60% ethanol. Intracellular cAMP concentrations were determined using an assay which measures the ability of cAMP to displace [8-


3


H] cAMP from a high affinity cAMP binding protein (see Gilman, 1979


, Proc. Natl. Acad. Sci. USA


67: 305-312).




The results of these experiments are shown in

FIGS. 11A through 11C

. The abscissa indicates the concentration of each hormone and the ordinate indicates the percentage of basal intracellular cAMP concentration achieved by each treatment. Points indicate the mean of duplicate incubations; the standard error did not exceed 15% for any data point. Panel A depicts the results of experiments using peptides found in vivo; Panel B depicts results found with γ-MSH variants; and Panel C shows results of synthetic melanocortin analogues. None of the peptides tested induced any change in intracellular cAMP in cells containing the vector alone. Cells expressing rat MC3-R responded strongly to every melanotropic peptide containing the MSH code sequence His-Phe-Arg-Trp, with up to a 60-fold elevation of intracellular cAMP levels. EC


50


values ranged from 1-50 nM. The most potent ligand and the one having the lowest EC


50


was found to be γMSH. The order of potency for the naturally occurring melanocortins was found to be:




γ


2


-MSH=γMSH>αMSH=ACTH


1-39





3


-MSH>des-acetyl-αMSH>ACTH


4-10


.




Ec


50


values for these compounds are shown in Table II:















TABLE II











Agonist




Ec


50















NDP-MSH




1 × 10


−9









γ


1


-MSH




3 × 10


−9









γ


2


-MSH




3 × 10


−9









α-MSH




4 × 10


−9









ACTH


1-39






4 × 10


−9









γ


3


-MSH




6 × 10


−9









desacetyl-αMSH




8 × 10


−9









ACTH


4-10






1 × 10


−7

















Additionally, a synthetic melanocortin peptide (ORG2766), known to have the greatest activity in vivo in stimulation of retention of learned behavior and in stimulation of neural regeneration, was unable to stimulate MC3-R-mediated cAMP production, and was also inactive as an antagonist. The results strongly indicate that this peptide does not bind to MC3-R protein.




The MC-4 receptor was cloned in a 1.9 kb HindIII genomic DNA fragment after PCR amplification of a lambda phage clone into pcDNAI/Neo (Invitrogen). This plasmid was stably introduced into human 293 cells by calcium phosphate co-precipitation using standard techniques, and plasmid-containing cells selected in G418 containing media. Specificity of receptor-hormone binding was assayed using adenylate cylcase activity as described above. The MC-4 receptor was found to couple to adenylate cyclase activity having the following pattern of agonist affinity:




NDP-MSH>des-acetyl-α-MSH>/=ACTH


1-39


>/=α-MSH>>γ


2


-MSH=ACTH


4-10






whereas the synthetic ACTH


4-9


analogue ORG2766 showed no detectable binding to the MC-4 receptor. The results of adenylate cyclase activity assays are shown in FIG.


12


. EC


50


values for each of the tested MC-4 receptor agonists are as shown in Table III:















TABLE III











Agonist




Ec


50















NDP-MSH




1.1 × 10


−11


M







desacetyl-αMSH




4.9 × 10


−10


M







ACTH


1-39






6.8 × 10


−10


M







α-MSH




1.5 × 10


−9


M







γ


2


-MSH




>10


−7


M







ACTH


4-10






>10


−7


M















A 1.6 kb ApaI-HindIII fragment comprising the entire coding sequence of the mouse MC-5 melanocortin receptor disclosed in Example 2G above was cloned into the pcDNA/neo expression vector (Invitrogen) after PCR amplification of the lambda phage clone. This plasmid was stably introduced into human 293 cells by calcium phosphate co-precipitation using standard techniques, and plasmid-containing cells selected in G418 containing media. Specificity of receptor-hormone binding was assayed using adenylate cylcase activity as described above. The MC-5 receptor was found to couple to adenylate cyclase activity having the following pattern of agonist affinity:




α-MSH>PMSH>>γ-MSH The results of adenylate cyclase activity assays (AC) and cAMP-dependent β-galactosidase (β-gal) assay are shown in FIG.


13


. EC


50


values for each of the tested MC-5 receptor agonists are: α-MSH=1.7×10


−9


M; and βMSH=5×10


−9


M.




EXAMPLE 4




Melanocortin Analogue Binding to Mammalian Melanocortin Receptors




Recombinant cells prepared as described above in Example 3 were used to characterize receptor binding of two melanocortin analogues comprising cyclic lactarn heptapeptides.




The melanocortin receptor analogue SHU9119 has the following chemical structure:











Ac-Nle


4


-cyclo(Asp


5


, D-Nal(2)


7


, Lys


10


) αMSH-(4-10)-amide The melanocortin receptor analogue MTII has the following chemical structure:











These analogues were prepared as described in Hruby et al. (1995


, J. Med. Chem


. 38: 3454-3461).




These analogues were tested for melanocortin receptor binding using a colorimetric assay system developed by some of the instant inventors (Chen et al., 1995


, Analyt. Biochem


. 226: 349-354) as follows. A series of concatamers of the synthetic oligonucleotide:




5′-GAATTCGACGTCACAGTATGACGGCCATGG-3′ (SEQ ID No:19)




was produced by self-annealing and ligation and a tandem tetramer obtained. This fragment was cloned upstream of a fragment of the human vasoactive intestinal peptide (−93-+152; SEQ ID No.:13; see Fink et al., 1988


, Proc. Natl. Acad. Sci. USA


85: 6662-6666). This promoter was then cloned upstream of the β-galactosidase gene from


E. coli


. The resulting plasmid construct is shown in FIG.


14


.




Transient transfection of the pCRE/β-gal plasmid described above was performed as follows. Cells at between 40-60% confluency (corresponding to about 1.5 million cells/6 cm tissue culture plate) were incubated with Opti-MEM (Gibco BRL, Long Island, N.Y.) and then contacted with a pCRE/β-gal-lipofectin complex which was prepared as follows. 3 μg plasmid DNA and 20 μL lipofectin reagent (Gibco) were each diluted into 0.5 mL Opti-MEM media and then mixed together. This mixture was incubated at room temperature for 15-20 min., and then the mixture (1 mL) added to each 6 cm plate. Transfected plates were incubated at 37° C. for 5-24 h, after which the plates were washed and incubated with DMEM media (Gibco) and the cells split equally into a 96-well culture plate.




To assay melanocortin receptor analogue binding, human 293 cells expressing each of the melanocortin receptors MC-1, MC-3, MC-4 and MC-5, and mouse Y1 cells expressing the MC-2 receptor, were transiently transfected with pCRE/β-gal as described above and assayed as follows. Two days after transfection, cells were stimulated with hormones specific for each receptor or hormone analogue by incubation for 6 h at 37° C. with a mixture comprising 10


−12


-10


−6


M hormone or analogue, 0.1 mg/mL bovine serum albumin and 0.1 mM isobutylmethylxanthine in DMEM. The effect of hormone or analogue binding was determined by β-galactosidase assay according to the method of Felgner et al. (1994


, J. Biol. Chem


. 269: 2550-2561). Briefly, media was aspirated from culture wells and 50 μL lysis buffer (0.25M Tris-HCl, pH 8/0.1% Triton-X100) added to each well. Cell lysis was enhanced by one round of freezing and thawing the cell/lysis buffer mixture. 10 μL aliquots were sampled from each well for protein determination using a commercially-available assay (BioRad, Hercules, Calif.). The remaining 40 μL from each well was diluted with 40 μL phosphate buffered saline/0.5% BSA and 150 μL substrate buffer (60 mM sodium phosphate/1 mM MgCl


2


/10 mM KCV/5 mM β-mercaptoethanol/200 μg/mL o-nitrophenyl-β-D-galactopyranoside) added. Plates were incubated at 37° C. for 1 h and then absorbance at 405 nm determined using a 96-well plate reader (Molecular Devices, Sunnyvale, Calif.). A series of two-fold dilutions from 20 ng of purified β-galactosidase protein (Sigma Chemical Co, St. Louis, Mo.) were assayed in parallel in each experiment to enable conversion of OD


405


to known quantity of β-galactosidase protein.




The results of these experiments are shown in FIG.


15


. This Figure shows the results of the β-galactosidase assay described above using cells expressing each of the MC-1, MC-3, MC-4 or MC-5 receptors and contacted with αMSH or a variety of αMSH analogues, including SHU9119. These results showed that SHU9119 had relatively weak agonist activity for both the human MC-3 and MC-4 receptors.




These results demonstrated the development of a colorimetric assay for cAMP accumulation as the result of melanocortin receptor binding to agonists and antagonists.




The action of MTII, SHU9119, and the endogenous mouse agouti peptide as agonists or antagonists of rodent MC receptors was determined by examining their ability to elevate intracellular cAMP in 293 cell lines expressing the rat MC3-R or mouse MC-4-R (expressed as IC


50


values representing ligand concentrations required for half-maximal inhibition of binding of


125


I-[Nle


4


,D-Phe


7


]α-MSH tracer). Agonist/antagonist activity was also shown by demonstrating inhibition of cAMP elevation by the potent α-MSH analogue [Nle


4


, D-Phe


7


]α-MSH, using either a cAMP-responsive β-galactosidase reporter construct as described above, or by direct adenyl cyclase assay as described in Example 3 (wherein EC


50


values represent ligand concentration required for half-maximal activation of a cAMP-responsive β-galactosidase reporter). Competition binding experiments were determined as the amount of radioactivity bound in the presence of 5×10


−6


M cold [Nle


4


, D-Phe


7


]α-MSH, and was typically 3-5% of total counts bound.




In these experiments, murine agouti peptide was produced using a baculovirus system as described by Lu et al., (1994


, Nature


371: 799-802), with the modification that the agouti peptide was purified from baculovirus supernatants by 0.6M NaCl step elution from an EconoS cation exchange column (BioRad). Agouti peptide used in these assays was approximately 60% pure.




Competition binding assays were performed to determine whether SHU9119 had antagonist activity. towards αMSH binding to either the MC-3 or MC-4 receptors. These assays were performed as follows. Human 293 cells (100,000 cells/well in 24-well plates) expressing either the MC-3 or MC-4 receptors prepared as described above were incubated with a solution of 1 mg/mL BSA in PBS containing 100,000 cpm (3.1×10


−10


M [


125


I](Nle


4


, D-Phe


7


)αMSH and varying concentration αMSH, (Nle


4


, D-Phe


7


)αMSH or SHU9119. Cells were incubated for 30 min at 37° C., washed twice with PBS-BSA, lysed with 0.5 mL 0.5N NaOH, and counted using a γ-counter to quantitate the amount of bound [


125


I](Nle


4


, D-Phe


7


)αMSH. Control experiments showed non-specific binding to occur at about 3-5% levels, and this was taken into account when analyzing the experimental results.




The SHU9119 analogue was found to be a potent antagonist of both the human MC-3 and MC-4 receptors, as shown in FIG.


16


. These assays showed pA


2


values of 8.3 and 9.3 for the human MC-3 and MC-4 receptors, respectively, as determined using the method of Schild (1947


, Brit. J. Pharmacol


. 2: 189-206). In contrast, no significant alteration in IC


50


values was detected in binding experiments using this analogue with either the MC-3 or MC-4 receptors (FIG.


17


).




The activity of the MTII analogue was also assayed for melanocortin receptor agonist activity. These results are shown in

FIGS. 18A and 18B

, and confirmed that the MTII analogue is a specific agonist of the MC-3 and MC-4 receptors.




Specific competition of [Nle


4


, D-Phe


7


]α-MSH binding to the rat MC-3 receptor by agouti peptide was observed, although accurate IC


50


values could not be determined because the peptide preparation was not homogeneous (results not shown). Specific competition of αMSH activation of human MC-4-R by agouti was previously disclosed (Lu et al., 1994


, Nature


371: 799-802).




EXAMPLE 5




Feeding Behavior Effect of Melanocortin Analogue Binding in Brain




The results shown in Example 4 above suggested a role in the regulation of feeding behavior in mammalian brain for MC receptor agonists and antagonists, in view of the antagonist properties of the agouti peptide at the MC-3 and MC-4 receptors. The agouti peptide was known to cause obesity when expressed ectopically in the mouse, and has been found to be a high affinity antagonist of the melanocyte stimulating hormone receptor (MC1-R) and of the hypothalamic MC-4 receptor (see Lu et al., ibid.). The former activity explained the inhibitory effect of the agouti peptide on eumelanin pigment synthesis. Similarly, it was hypothesized by the inventors that agouti causes obesity in mice by antagonizing hypothalamic MC-4 receptors. The cyclic melanocortin analogue, SHU9119, having been shown herein and elsewhere (Hruby et al.) to be a specific, high affinity antagonist of the central MC-3 and MC-4 receptors, was tested for the effect of direct administration to mouse brain on feeding behavior in the. animals. Intracerebroventricular (ICV) administration of SHU9119 was performed to avoid any complications caused by inhibition of peptide traverse of the blood-brain barrier.




Briefly, male C57B1/6J mice (18-29 g) were maintained on a normal 12 hr/12 hr light dark cycle with food (Purina mouse chow) and water ad libitum. Animals were housed individually for 24 hrs, distributed into experimental and control groups, avoiding any bias as a function of prior weight, then injected with vehicle or vehicle plus drug just prior to the onset of a 12 hr light or dark cycle. Fasted animals were deprived of food from 18:00 to 10:30 hrs to stimulate feeding during the daytime experimental period. Animals were lightly anesthetized with halothane, and administered into one lateral ventricle 2 μL of a solution of artificial cerebrospinal fluid alone (acsf, comprising 130 mM NaCl, 27 mM NaHCO


3


, 1.2 mM Na


2


HPO


4


, 0.3 mM NaH


2


PO


4


, 0.5 mM Na


2


SO


4


, 1.0 mM CaCl


2


, 1.0 mM MgCl


2


, and 2.5 mM KCl), or 6 nmol SHU9119 in acsf. Freehand injections were performed as described by Laursen and Belkunap (1986


, J. Pharmacol. Methods


16: 355-357) with some modifications. A 10 μl luertip syringe (Hamilton 701LT) was fitted with a 0.5 inch 27 gauge needle. Stiff Tygon tubing was slipped over the needle to expose 3 mM of the needle tip. The syringe was held at a 45° angle from the front of the skull with the bevel facing up. The coronal suture was found by lightly rubbing the needle over the skull. Maintaining the 45° angle, the needle was then inserted 1-2 mm lateral to the midline, using only mild pressure to insert and remove the needle. The compounds indicated in a 2 μl volume of acsf were administered slowly over approximately 15 seconds, and the needle removed after 35 seconds. Animals were allowed to recover from anesthesia and placed into a cage containing a premeasured quantity of food pellets in a spill-free cup. Moribund animals were not included in the study.




Stimulation of feeding by intracerebroventricular administration of the melan-cortin antagonist SHU9119 is shown in

FIGS. 19A through 19C

. Curves show cumulative food intake as a function of time following administration of the substances shown.

FIG. 19A

shows stimulation of feeding by administration of SHU9119just priorto lights off(19:00 hrs) to C57B1/6J mice fed ad libidum.

FIG. 19B

, in contrast, shows no effect of morning (10:00 hrs) SHU9119 administration in C57B1/6J mice fed ad libidum.

FIG. 19C

illustrates stimulation of daytime feeding by SHU9119 administration in fasted C57B1/6J mice. In deriving the data points comprising these FIGS., food remaining was briefly removed and weighted at the time intervals indicated. Data points indicate the mean and bars indicate standard error. Significance of the effect over time was determined by ANOVA with repeated measures. Significance of drug effects at individual time points was determined by two-way ANOVA, and is indicated in each Figure (***=P<0.001, **=P<0.01, *=P<0.05).




These results demonstrated that ICV administration of SHU9119 into one lateral ventricle of the C57B1/6J mouse just prior to lights out led to a mean 60% increase in food intake over 12 hrs (

FIG. 19A

; P<0.005). In contrast, daytime food intake in animals fed ad libidum was not stimulated by administration of SHU9119 (FIG.


19


B). SHU9119-treatment did, however, significantly stimulate daytime food intake in animals fasted for 16 hrs prior to the experiment (

FIG. 19C

; P<0.001). Stimulation of feeding was evident at approximately two hrs post-treatment, and continued for 12 hrs, to produce a mean 34% in food intake relative to vehicle-injected controls.




These results supported the hypothesis that agouti or SHU9119 stimulate feeding by antagonizing MC receptors in the central nervous system. To further test this hypothesis, a series of experiments were performed wherein MC receptor agonists were administered to animals primed by fasting to eat, to determine whether feeding in such animals could be inhibited by the MC receptor agonists. Animals were induced to feed by food deprivation for 16 h prior to ICV administration of the non-specific melanocortin agonist MTII. In these experiments, ICV injections in male C57B1/6J mice (20-30 g) and the measurement of food intake were as described above.




Results of these experiments are shown in

FIGS. 20A through 20C

. In comparison to vehicle-injected animals, MTII was found to produce a potent inhibition of feeding within one hour after administration (

FIG. 20A

) in a dose-responsive manner. Food intake was significantly inhibited for up to four hours following administration (P<0.001) at the highest dose administered (3 nmol), and decreased food intake continued for the next four hours with normal rates of food intake resuming at about 8 hours after treatment. This dose-responsive inhibition of feeding had an IC


50


at the two hour time point of approximately 0.5 nmol (FIG.


20


C). However, inhibition of feeding with 3 nmol MTII was completely blocked by co-administration of 6 nmol SHU9119 (

FIG. 20B

; P<0.001), demonstrating that the effect results specifically from agonist binding to the MC-4 and/or MC-3 receptor.




Locomotor assays were performed to determine whether the effects on feeding behavior observed in these mice were secondary to generalized behavioral effects caused by administration of these melanocortin analogues. The effects of MTII on locomotor activity were tested by placing vehicle or MTII-treated mice in sound and light-proof cages containing multiple light beam detectors. These assays were performed by first injecting 3 nmol MTII or acsf as described above. At three hours (2:45-3:25) post-injection, 12 mice were placed into 12 separate boxes containing multiple infrared light sources and photodetectors. The boxes were contained within separate ventilated light and sound attenuating chambers (Coulboum model E10-20). Disruption of the infrared beams, with a 10 msec. resolution, was tallied independently for each one minute time period in each cage. The results of these assays are shown in FIG.


20


D. Data points indicate the mean total activity (# of light breaks) for 6 animals in each experimental group. Four way Anova statistical analysis was used to analyze the data, and indicated an absence of a significant difference among the two groups.




Inhibition of feeding by MTII could not be explained by any apparent behavioral abnormalities, or any effect on arousal or locomotor activity. MTII-treated animals appeared alert and exhibited no unusual behavior relative to controls. At approximately three hours after ICV administration, MTII-treated animals exhibited locomotor activity that was indistinguishable from vehicle-treated animals (FIG.


20


D). The higher initial activity, indicative of exploratory behavior, and continued locomotion over a 15 min period was indistinguishable between the two groups, indicating that the inhibition of feeding was not due to decreased locomotion or decreased arousal.




The administration of MTII also inhibited food intake in three other models of hyperphagia: the C57B1/6J-Lep


ob


mouse, C57B1/6J-A


Y


mouse, and NPY-injected C57B1/6J mouse.

FIG. 21A

shows inhibition of feeding by intracerebroventricular administration of MTII in A


Y


mice (females, 19-28 gms).

FIG. 21B

shows inhibition of feeding by intracerebroventricular administration of MTII in C57B1/6J mice (females, 21-25 gm) stimulated to feed by co-administration of neuropeptide Y.

FIG. 21C

shows inhibition of feeding by intracerebroventricular administration of the MTII in ob/ob mice (females, 48-69 gms).

FIG. 21D

shows inhibition of feeding in ob/ob mice intraperitoneal administration of MTII (females, 40-45 gms). ICV injections and measurement of food intake was performed as described above, with the exception of NPY treated animals, which were not fasted prior to experimentation. Animals treated intraperitoneally received 100 μl of a 1 mM solution of MTII in saline, and vehicle injections consisted of the same value of saline alone. Significance indicated for individual time points, determined as described above, was for 3 nmol MTII vs. acsf(FIG.


21


A), 1.18 nmol NPY vs. 1.18 nmol NPY+3 nmol MTII (FIG.


21


B), 3 nmol MTII vs. acsf (FIG.


21


C), and 100 nmol MTII vs. saline (FIG.


21


D).




The hyperphagia in these models can be clearly seen by comparing the 12 hr food intake following a fast in vehicle-injected C57B1/6J (2.4 g, FIG.


19


A), C57B1/6J-A


Y


(3.7 g,

FIG. 21A

) and C57B1/6J-Lep


ob


(3.7 g.

FIG. 21C

) animals. As expected, MTII treatment inhibited food intake following a 16 hr fast in the C57B1/6J-A


Y


mouse (

FIG. 21A

; P<0.05). Interestingly, while food intake for the first four hours is significantly inhibited relative to vehicle-injected animals, it is also significantly less inhibited in the C57B1/6J-A


Y


animal than in the C57B1/6J animal given the same 3 nmol dose (compare,

FIG. 20A

versus

FIG. 21A

, 1-4 hrs; P<0.001). The decreased effectiveness of the agonist in the presence of the A


Y


allele is consistent with the proposal that his allele results in chronic expression of agouti peptide melanocortin antagonist in the brain.




MTII, upon co-administration, also significantly inhibited the profound stimulation of feeding induced by neuropeptide Y (NPY), measured over a three hr period (

FIG. 21C

; P<0.005). Co-administration of an approximately two-fold molar excess of MTII produced a 74% inhibition of NPY-stimulated food intake at the three hour time point.




Finally, MTII also inhibited hyperphagia due to absence of leptin in the C57B1/6J-Lep


ob


mouse (

FIG. 21C

; P<0.001). MTII blocked feeding as potently (FIG.


20


A), in contrast to the less potent inhibition described above for the C57B1/6J-A


Y


mouse.




The C57B1/6J-Lep


ob


animal was also used to test the ability of MTII to regulate feeding when administered peripherally. Moderate doses (100 nmol) of MTII were inhibited feeding in the C57B1/6J-Lep


ob


mouse (P<0.001) while low doses (10 nmol) did not (date not shown). The kinetics were similar to those seen with ICV administration, with a potent inhibition of feeding for the first four hours. The 100-fold higher dose required peripherally, as well as the similar kinetics suggest a primarily central mechanism of action of MTII.




These data show that melanocortinergic neurons exert a tonic inhibition of feeding behavior, and that disruption of this signal leads to hyperphagia. With regard to the recently-discovered leptin hormone made by adipocytes, which is generally expressed at elevated levels in obese humans and rodents (such as the C57B1/6J-Lep


ob


animal), the regulatory defect is understood to be an incapacity to respond properly to the leptin hormone signal. The instant results indicate that the melanocortins act independently, or physiologically “downstream,” from the leptin hormone/receptor interaction, because it has been shown herein that melanocortin receptor agonists can potently inhibit feeding in the C57B1/6J-Lep


ob


animal.




These results suggest that MC receptor agonists and antagonists can affect mammalian feeding behavior, and provide a means for determining candidate compounds for the development of effective pharmacological products directed towards alleviating such human ailments as obesity, anorexia and cachexia.




EXAMPLE 6




Use of MC Receptor-Expressing Recombinant Cells for Screening Compounds that Affect Feeding Behavior in Mammals




The results obtained in Example 6 indicated that cells expressing a variety of mammalian melanocortin receptors are useful for characterizing compounds as a first step towards developing MC receptor agonists and antagonists for controlling feeding behavior in mammals, particularly obesity and overweight disorders in general, as well as anorexia, cachexia and other failure-to-thrive disorders.




A panel of mammalian melanocortin receptor-expressing recombinant cells are provided as described above in Example 3, wherein each member of the panel comprises appropriate mammalian cells, such as human 293 cells, comprising a recombinant expression construct encoding the MC-1, MC-2 (ACTH), MC-3, MC-4 or MC-5 receptor, the panel constructed to comprise cells functionally expressing each of these MC receptor proteins.




The panel is used as follows. Receptor agonist activity is assayed by transient or stable expression of a protein which produces a metabolite reporter molecule in response to receptor binding by any of the MC receptor proteins. An example of such a reporter system is the recombinant expression construct described in Example 4, wherein cAMP responsive elements (CREs) are operatively linked to bacterially-derived β-galactosidase (β-gal) gene. In the event of receptor binding, cAMP is produced in the mammalian cell, and the CRE induces β-gal expression. When co-incubated with a colorless substrate for β-gal, receptor binding results in conversion of the colorless substrate to a blue-colored product, which can be easily scored visually or spectrophotometrically. Alternative reporter genes, such a luciferase, can also be used as reporter systems, provided that expression of the reporter molecule-producing protein is functionally linked to receptor binding of a test compound. Alternatively, cAMP production resulting from MC receptor binding can also be measured directly. Additionally, the cell panel or membranes from these cells can be used for direct radioligand binding assays.




Assay panels are arranged so that agonist activity can be identified, quantitated and correlated with expression of each MC receptor. Automated assays using such panels are also envisioned, whereby the qualitative and quantitative detection of a reporter metabolite is detected in an array (such as a 96-well tissue culture plate) and the data collected and assembled into a computer data-base or other analytical program.




Antagonist activity is detected by a modification of the above assay. In this assay, the inhibition of production of an amount of a known receptor agonist, specific for each receptor, is assayed in the presence of a putative antagonist compound. Production of metabolite reporter molecules and their qualitative and quantitative detection is achieved as described above, and the specificity and potency of each antagonist compound characterized with regard to the degree of inhibition achieved for each receptor.




In view of the instant disclosure, MC-3/MC-4 receptor antagonists are expected to be useful to inhibit food intake in a hungry animal, and MC-3/MC-4 receptor agonists are expected to be useful to increase food intake in an animal. Alternative patterns of feeding behavior associated with different patterns of MC receptor agonist/antagonist activity can be determined using this assay.




Compounds having agonist or antagonist activity with the MC-3 or MC-4 receptors detected using this assay are further screened in vivo to determine whether the observed receptor binding activity results in modification of feeding behavior when administered to an animal. In these assays, the MC receptor binding compounds detected using the assay are administered intracerebroventricularly as described above in Example 5 to animals after an overnight fast, to waking animals, or to animals that are not otherwise primed to be hungry. Feeding and locomotor activity is monitored in these animals, and compounds affecting eating behavior (either by inhibiting feeding in otherwise hungry animals or stimulating feeding in otherwise sated animals) are selected for further development.




In addition, systemic administration of compounds found to be active by ICV administration assays is used to screen such compounds for the ability to cross the blood-brain barrier. Such compounds are also useful as templates for modifications aimed at increasing the availability of these compounds in the brain after systemic administration, for increasing bioactivity, or both.




It should be understood that the foregoing disclosure emphasizes certain specific embodiments of the invention and that all modifications or alternatives equivalent thereto are within the spirit and scope of the invention as set forth in the appended claims.







19





35 base pairs


nucleic acid


single


linear




DNA (genomic)




misc_feature


1..35



/function = “Degenerate
oligonucleotide primer (sense)”
/note= “The residue at positions 24 and 24 are
inosine”





1
GAGTCGACCT GTGYGYSATY RCNNTKGACM GSTAC 35






32 base pairs


nucleic acid


single


linear




DNA (genomic)




misc_feature


1..32



/function = “Degenerate
oligonucleotide primer (antisense)”
/note= “The residue at position 18 is inosine”





2
CAGAATTCAG WAGGGCANCC AGCAGASRYG AA 32






1260 base pairs


nucleic acid


single


linear




cDNA to mRNA




5′UTR


1..14





CDS


15..959





3′UTR


960..1260




3
TTCCTGACAA GACT ATG TCC ACT CAG GAG CCC CAG AAG AGT CTT CTG GGT 50
Met Ser Thr Gln Glu Pro Gln Lys Ser Leu Leu Gly
1 5 10
TCT CTC AAC TCC AAT GCC ACC TCT CAC CTT GGA CTG GCC ACC AAC CAG 98
Ser Leu Asn Ser Asn Ala Thr Ser His Leu Gly Leu Ala Thr Asn Gln
15 20 25
TCA GAG CCT TGG TGC CTG TAT GTG TCC ATC CCA GAT GGC CTC TTC CTC 146
Ser Glu Pro Trp Cys Leu Tyr Val Ser Ile Pro Asp Gly Leu Phe Leu
30 35 40
AGC CTA GGG CTG GTG AGT CTG GTG GAG AAT GTG CTG GTT GTG ATA GCC 194
Ser Leu Gly Leu Val Ser Leu Val Glu Asn Val Leu Val Val Ile Ala
45 50 55 60
ATC ACC AAA AAC CGC AAC CTG CAC TCG CCC ATG TAT TAC TTC ATC TGC 242
Ile Thr Lys Asn Arg Asn Leu His Ser Pro Met Tyr Tyr Phe Ile Cys
65 70 75
TGC CTG GCC CTG TCT GAC CTG ATG GTA AGT GTC AGC ATC GTG CTG GAG 290
Cys Leu Ala Leu Ser Asp Leu Met Val Ser Val Ser Ile Val Leu Glu
80 85 90
ACT ACT ATC ATC CTG CTG CTG GAG GTG GGC ATC CTG GTG GCC AGA GTG 338
Thr Thr Ile Ile Leu Leu Leu Glu Val Gly Ile Leu Val Ala Arg Val
95 100 105
GCT TTG GTG CAG CAG CTG GAC AAC CTC ATT GAC GTG CTC ATC TGT GGC 386
Ala Leu Val Gln Gln Leu Asp Asn Leu Ile Asp Val Leu Ile Cys Gly
110 115 120
TCC ATG GTG TCC AGT CTC TGC TTC CTG GGC ATC ATT GCT ATA GAC CGC 434
Ser Met Val Ser Ser Leu Cys Phe Leu Gly Ile Ile Ala Ile Asp Arg
125 130 135 140
TAC ATC TCC ATC TTC TAT GCG CTG CGT TAT CAC AGC ATC GTG ACG CTG 482
Tyr Ile Ser Ile Phe Tyr Ala Leu Arg Tyr His Ser Ile Val Thr Leu
145 150 155
CCC AGA GCA CGA CGG GCT GTC GTG GGC ATC TGG ATG GTC AGC ATC GTC 530
Pro Arg Ala Arg Arg Ala Val Val Gly Ile Trp Met Val Ser Ile Val
160 165 170
TCC AGC ACC CTC TTT ATC ACC TAC TAC AAG CAC ACA GCC GTT CTG CTC 578
Ser Ser Thr Leu Phe Ile Thr Tyr Tyr Lys His Thr Ala Val Leu Leu
175 180 185
TGC CTC GTC ACT TTC TTT CTA GCC ATG CTG GCA CTC ATG GCG ATT CTG 626
Cys Leu Val Thr Phe Phe Leu Ala Met Leu Ala Leu Met Ala Ile Leu
190 195 200
TAT GCC CAC ATG TTC ACG AGA GCG TGC CAG CAC GTC CAG GGC ATT GCC 674
Tyr Ala His Met Phe Thr Arg Ala Cys Gln His Val Gln Gly Ile Ala
205 210 215 220
CAG CTC CAC AAA AGG CGG CGG TCC ATC CGC CAA GGC TTC TGC CTC AAG 722
Gln Leu His Lys Arg Arg Arg Ser Ile Arg Gln Gly Phe Cys Leu Lys
225 230 235
GGT GCT GCC ACC CTT ACT ATC CTT CTG GGG ATT TTC TTC CTG TGC TGG 770
Gly Ala Ala Thr Leu Thr Ile Leu Leu Gly Ile Phe Phe Leu Cys Trp
240 245 250
GGC CCC TTC TTC CTG CAT CTC TTG CTC ATC GTC CTC TGC CCT CAG CAC 818
Gly Pro Phe Phe Leu His Leu Leu Leu Ile Val Leu Cys Pro Gln His
255 260 265
CCC ACC TGC AGC TGC ATC TTC AAG AAC TTC AAC CTC TTC CTC CTC CTC 866
Pro Thr Cys Ser Cys Ile Phe Lys Asn Phe Asn Leu Phe Leu Leu Leu
270 275 280
ATC GTC CTC AGC TCC ACT GTT GAC CCC CTC ATC TAT GCT TTC CGC AGC 914
Ile Val Leu Ser Ser Thr Val Asp Pro Leu Ile Tyr Ala Phe Arg Ser
285 290 295 300
CAG GAG CTC CGC ATG ACA CTC AAG GAG GTG CTG CTG TGC TCC TGG 959
Gln Glu Leu Arg Met Thr Leu Lys Glu Val Leu Leu Cys Ser Trp
305 310 315
TGATCAGAGG GCGCTGGGCA GAGGGTGACA GTGATATCCA GTGGCCTGCA TCTGTGAGAC 1019
CACAGGTACT CATCCCTTCC TGATCTCCAT TTGTCTAAGG GTCGACAGGA TGAGCTTTAA 1079
AATAGAAACC CAGAGTGCCT GGGGCCAGGA GAAAGGGTAA CTGTGACTGC AGGGCTCACC 1139
CAGGGCAGCT ACGGGAAGTG GAGGAGACAG GGATGGGAAC TCTAGCCCTG AGCAAGGGTC 1199
AGACCACAGG CTCCTGAAGA GCTTCACCTC TCCCCACCTA CAGGCAACTC CTGCTCAAGC 1259
C 1260






315 amino acids


amino acid


linear




protein



4
Met Ser Thr Gln Glu Pro Gln Lys Ser Leu Leu Gly Ser Leu Asn Ser
1 5 10 15
Asn Ala Thr Ser His Leu Gly Leu Ala Thr Asn Gln Ser Glu Pro Trp
20 25 30
Cys Leu Tyr Val Ser Ile Pro Asp Gly Leu Phe Leu Ser Leu Gly Leu
35 40 45
Val Ser Leu Val Glu Asn Val Leu Val Val Ile Ala Ile Thr Lys Asn
50 55 60
Arg Asn Leu His Ser Pro Met Tyr Tyr Phe Ile Cys Cys Leu Ala Leu
65 70 75 80
Ser Asp Leu Met Val Ser Val Ser Ile Val Leu Glu Thr Thr Ile Ile
85 90 95
Leu Leu Leu Glu Val Gly Ile Leu Val Ala Arg Val Ala Leu Val Gln
100 105 110
Gln Leu Asp Asn Leu Ile Asp Val Leu Ile Cys Gly Ser Met Val Ser
115 120 125
Ser Leu Cys Phe Leu Gly Ile Ile Ala Ile Asp Arg Tyr Ile Ser Ile
130 135 140
Phe Tyr Ala Leu Arg Tyr His Ser Ile Val Thr Leu Pro Arg Ala Arg
145 150 155 160
Arg Ala Val Val Gly Ile Trp Met Val Ser Ile Val Ser Ser Thr Leu
165 170 175
Phe Ile Thr Tyr Tyr Lys His Thr Ala Val Leu Leu Cys Leu Val Thr
180 185 190
Phe Phe Leu Ala Met Leu Ala Leu Met Ala Ile Leu Tyr Ala His Met
195 200 205
Phe Thr Arg Ala Cys Gln His Val Gln Gly Ile Ala Gln Leu His Lys
210 215 220
Arg Arg Arg Ser Ile Arg Gln Gly Phe Cys Leu Lys Gly Ala Ala Thr
225 230 235 240
Leu Thr Ile Leu Leu Gly Ile Phe Phe Leu Cys Trp Gly Pro Phe Phe
245 250 255
Leu His Leu Leu Leu Ile Val Leu Cys Pro Gln His Pro Thr Cys Ser
260 265 270
Cys Ile Phe Lys Asn Phe Asn Leu Phe Leu Leu Leu Ile Val Leu Ser
275 280 285
Ser Thr Val Asp Pro Leu Ile Tyr Ala Phe Arg Ser Gln Glu Leu Arg
290 295 300
Met Thr Leu Lys Glu Val Leu Leu Cys Ser Trp
305 310 315






1633 base pairs


nucleic acid


single


linear




cDNA to mRNA




5′UTR


1..461





CDS


462..1415





3′UTR


1416..1633




5
CCCGCATGTG GCCGCCCTCA ATGGAGGGCT CTGAGAACGA CTTTTAAAAC GCAGAGAAAA 60
AGCTCCATTC TTCCCAGACC TCAGCGCAGC CCTGGCCCAG GAAGGGAGGA GACAGAGGCC 120
AGGACGGTCC AGAGGTGTCG AAATGTCCTG GGAACCTGAG CAGCAGCCAC CAGGGAAGAG 180
GCAGGGAGGG AGCTGAGGAC CAGGCTTGGT TGTGAGAATC CCTGAGCCCA GGCGGTTGAT 240
GCCAGGAGGT GTCTGGACTG GCTGGGCCAT GCCTGGGCTG ACCTGTCCAG CCAGGGAGAG 300
GGTGTGAGGG CAGATCTGGG GGTGCCCAGA TGGAAGGAGG CAGGCATGGG GACACCCAAG 360
GCCCCCTGGC AGCACCATGA ACTAAGCAGG ACACCTGGAG GGGAAGAACT GTGGGGACCT 420
GGAGGCCTCC AACGACTCCT TCCTGCTTCC TGGACAGGAC T ATG GCT GTG CAG 473
Met Ala Val Gln
1
GGA TCC CAG AGA AGA CTT CTG GGC TCC CTC AAC TCC ACC CCC ACA GCC 521
Gly Ser Gln Arg Arg Leu Leu Gly Ser Leu Asn Ser Thr Pro Thr Ala
5 10 15 20
ATC CCC CAG CTG GGG CTG GCT GCC AAC CAG ACA GGA GCC CGG TGC CTG 569
Ile Pro Gln Leu Gly Leu Ala Ala Asn Gln Thr Gly Ala Arg Cys Leu
25 30 35
GAG GTG TCC ATC TCT GAC GGG CTC TTC CTC AGC CTG GGG CTG GTG AGC 617
Glu Val Ser Ile Ser Asp Gly Leu Phe Leu Ser Leu Gly Leu Val Ser
40 45 50
TTG GTG GAG AAC GCG CTG GTG GTG GCC ACC ATC GCC AAG AAC CGG AAC 665
Leu Val Glu Asn Ala Leu Val Val Ala Thr Ile Ala Lys Asn Arg Asn
55 60 65
CTG CAC TCA CCC ATG TAC TGC TTC ATC TGC TGC CTG GCC TTG TCG GAC 713
Leu His Ser Pro Met Tyr Cys Phe Ile Cys Cys Leu Ala Leu Ser Asp
70 75 80
CTG CTG GTG AGC GGG ACG AAC GTG CTG GAG ACG GCC GTC ATC CTC CTG 761
Leu Leu Val Ser Gly Thr Asn Val Leu Glu Thr Ala Val Ile Leu Leu
85 90 95 100
CTG GAG GCC GGT GCA CTG GTG GCC CGG GCT GCG GTG CTG CAG CAG CTG 809
Leu Glu Ala Gly Ala Leu Val Ala Arg Ala Ala Val Leu Gln Gln Leu
105 110 115
GAC AAT GTC ATT GAC GTG ATC ACC TGC AGC TCC ATG CTG TCC AGC CTC 857
Asp Asn Val Ile Asp Val Ile Thr Cys Ser Ser Met Leu Ser Ser Leu
120 125 130
TGC TTC CTG GGC GCC ATC GCC GTG GAC CGC TAC ATC TCC ATC TTC TAC 905
Cys Phe Leu Gly Ala Ile Ala Val Asp Arg Tyr Ile Ser Ile Phe Tyr
135 140 145
GCA CTG CGC TAC CAC AGC ATC GTG ACC CTG CCG CGG GCG CCG CGA GCC 953
Ala Leu Arg Tyr His Ser Ile Val Thr Leu Pro Arg Ala Pro Arg Ala
150 155 160
GTT GCG GCC ATC TGG GTG GCC AGT GTC GTC TTC AGC ACG CTC TTC ATC 1001
Val Ala Ala Ile Trp Val Ala Ser Val Val Phe Ser Thr Leu Phe Ile
165 170 175 180
GGC TAC TAC GAC CAC GTG GCC GTC CTG CTG TGC CTC GTG GTC TTC TTC 1049
Gly Tyr Tyr Asp His Val Ala Val Leu Leu Cys Leu Val Val Phe Phe
185 190 195
CTG GCT ATG CTG GTG CTC ATG GCC GTG CTG GAC GTC CAC ATG CTG GCC 1097
Leu Ala Met Leu Val Leu Met Ala Val Leu Asp Val His Met Leu Ala
200 205 210
CGG GCC TGC CAG CAC GCC CAG GGC ATC GCC CGG CTC CAC AAG AGG CAG 1145
Arg Ala Cys Gln His Ala Gln Gly Ile Ala Arg Leu His Lys Arg Gln
215 220 225
CGC CCG GTC CAC CAG GGC TTT GGC CTT AAA GGC GCT GTC ACC CTC ACC 1193
Arg Pro Val His Gln Gly Phe Gly Leu Lys Gly Ala Val Thr Leu Thr
230 235 240
ATC CTG CTG GGC ATT TTC TTC CTC TGC TGG GGC CCC TTC TTC CTG CAT 1241
Ile Leu Leu Gly Ile Phe Phe Leu Cys Trp Gly Pro Phe Phe Leu His
245 250 255 260
CTC ACA CTC ATC GTC CTC TGC CCC GAG CAC CCC ACG TGC GGC TGC ATC 1289
Leu Thr Leu Ile Val Leu Cys Pro Glu His Pro Thr Cys Gly Cys Ile
265 270 275
TTC AAG AAC TTC AAC CTC TTT CTC GCC CTC ATC ATC TGC AAT GCC ATC 1337
Phe Lys Asn Phe Asn Leu Phe Leu Ala Leu Ile Ile Cys Asn Ala Ile
280 285 290
ATC GAC CCC CTC ATC TAC GCC TTC CAC AGC CAG GAG CTC CGC AGG ACG 1385
Ile Asp Pro Leu Ile Tyr Ala Phe His Ser Gln Glu Leu Arg Arg Thr
295 300 305
CTC AAG GAG GTG CTG ACA TGC TCC TGG TGA GCGCGGTGCA CGCGCTTTAA 1435
Leu Lys Glu Val Leu Thr Cys Ser Trp *
310 315
GTGTGCTGGG CAGAGGGAGG TGGTGATATT GTGGTCTGGT TCCTGTGTGA TGACCCTGGG1495
TTCCTTACCT CCCTGGTCCC CGTTTGTCAA AGAGGATGGA CTAAATGATC TCTGAAAG1555
TTGAAGCGCG GACCCTTCTG GGCAGGGAGG GGTCCTGCAA AACTCCAGGC AGGACTT1615
ACCAGCAGTC GTCGTGGGAA C 1633






317 amino acids


amino acid


linear




protein



6
Met Ala Val Gln Gly Ser Gln Arg Arg Leu Leu Gly Ser Leu Asn Ser
1 5 10 15
Thr Pro Thr Ala Ile Pro Gln Leu Gly Leu Ala Ala Asn Gln Thr Gly
20 25 30
Ala Arg Cys Leu Glu Val Ser Ile Ser Asp Gly Leu Phe Leu Ser Leu
35 40 45
Gly Leu Val Ser Leu Val Glu Asn Ala Leu Val Val Ala Thr Ile Ala
50 55 60
Lys Asn Arg Asn Leu His Ser Pro Met Tyr Cys Phe Ile Cys Cys Leu
65 70 75 80
Ala Leu Ser Asp Leu Leu Val Ser Gly Thr Asn Val Leu Glu Thr Ala
85 90 95
Val Ile Leu Leu Leu Glu Ala Gly Ala Leu Val Ala Arg Ala Ala Val
100 105 110
Leu Gln Gln Leu Asp Asn Val Ile Asp Val Ile Thr Cys Ser Ser Met
115 120 125
Leu Ser Ser Leu Cys Phe Leu Gly Ala Ile Ala Val Asp Arg Tyr Ile
130 135 140
Ser Ile Phe Tyr Ala Leu Arg Tyr His Ser Ile Val Thr Leu Pro Arg
145 150 155 160
Ala Pro Arg Ala Val Ala Ala Ile Trp Val Ala Ser Val Val Phe Ser
165 170 175
Thr Leu Phe Ile Gly Tyr Tyr Asp His Val Ala Val Leu Leu Cys Leu
180 185 190
Val Val Phe Phe Leu Ala Met Leu Val Leu Met Ala Val Leu Asp Val
195 200 205
His Met Leu Ala Arg Ala Cys Gln His Ala Gln Gly Ile Ala Arg Leu
210 215 220
His Lys Arg Gln Arg Pro Val His Gln Gly Phe Gly Leu Lys Gly Ala
225 230 235 240
Val Thr Leu Thr Ile Leu Leu Gly Ile Phe Phe Leu Cys Trp Gly Pro
245 250 255
Phe Phe Leu His Leu Thr Leu Ile Val Leu Cys Pro Glu His Pro Thr
260 265 270
Cys Gly Cys Ile Phe Lys Asn Phe Asn Leu Phe Leu Ala Leu Ile Ile
275 280 285
Cys Asn Ala Ile Ile Asp Pro Leu Ile Tyr Ala Phe His Ser Gln Glu
290 295 300
Leu Arg Arg Thr Leu Lys Glu Val Leu Thr Cys Ser Trp
305 310 315






2012 base pairs


nucleic acid


single


linear




cDNA to mRNA




5′UTR


1..693





CDS


694..1587





3′UTR


1588..2012




7
ACAACACTTT ATATATATTT TTATAAATGT AAGGGGTACA AAGGTGCCAT TTTGTTACAT 60
GGATATACCG TGTAGTGGTG AAGCCTGGGC TTTTAGTGTA TCTGTCATCA GAATAACATA 120
CGTGTTACCC ATAGGAATTT CTCATCACCC GCCCCCTCCA CCCTTCGAGT CTCCAATGTC 180
CATTCCACAC TCTATATCCA CGTGTATGCA TATAGCTCCA CATATAAGTG AGAACATGTA 240
GTATTTGACT TCCTCTTTCT GAGTTATTTC ACTTTGATAA TGGCCTCCAC TTCCATCCAT 300
GTTGCTGCAA AAGACATGAC CTTATTCTTT TTGATAGCTG GGGAGTACTC CATTGTGTAT 360
ATGTACCACA TTTCTTTATC CATTCACCCA TTGAGAACAC TTAGTTGATT CCATATCTTT 420
GCTATTGTCA CTAGTGCTGC AATAAACATA CATGTGCAGG CTCCTTCTAA TATACTGATT 480
TATATTTTAT GGAGAGAGAT AGAGTTCTTA GCGAGTGTGC TGTTTATTTC TAGTGTACTT 540
GCAACTAATA TTCTGTATAC TCCCTTTAGG TGATTGGAGA TTTAACTTAG ATCTCCAGCA 600
AGTGCTACAA GAAGAAAAGA TCCTGAAGAA TCAATCAAGT TTCCGTGAAG TCAAGTCCAA 660
GTAACATCCC CGCCTTAACC ACAAGCAGGA GAA ATG AAG CAC ATT ATC AAC TCG 714
Met Lys His Ile Ile Asn Ser
1 5
TAT GAA AAC ATC AAC AAC ACA GCA AGA AAT AAT TCC GAC TGT CCT CGT 762
Tyr Glu Asn Ile Asn Asn Thr Ala Arg Asn Asn Ser Asp Cys Pro Arg
10 15 20
TGT GTT TTG CCG GAG GAG ATA TTT TTC ACA ATT TCC ATT GTT GGA GTT 810
Cys Val Leu Pro Glu Glu Ile Phe Phe Thr Ile Ser Ile Val Gly Val
25 30 35
TTG GAG AAT CTG ATC GTC CTG CTG GCT GTG TTC AAG AAT AAG AAT CTC 858
Leu Glu Asn Leu Ile Val Leu Leu Ala Val Phe Lys Asn Lys Asn Leu
40 45 50 55
CAG GCA CCC ATG TAC TTT TTC ATC TGT AGC TTG GCC ATA TCT GAT ATG 906
Gln Ala Pro Met Tyr Phe Phe Ile Cys Ser Leu Ala Ile Ser Asp Met
60 65 70
CTG GGC AGC CTA TAT AAG ATC TTG GAA AAT ATC CTG ATC ATA TTG AGA 954
Leu Gly Ser Leu Tyr Lys Ile Leu Glu Asn Ile Leu Ile Ile Leu Arg
75 80 85
AAC ATG GGC ATA CTC AAG CCA CGT GGC AGT TTT GAA ACC ACA GCC CAT 1002
Asn Met Gly Ile Leu Lys Pro Arg Gly Ser Phe Glu Thr Thr Ala His
90 95 100
GAC ATC ATC GAC TCC CTG TTT CTG CTC TCC CGT CTT GGC TCC ATC TTC 1050
Asp Ile Ile Asp Ser Leu Phe Leu Leu Ser Arg Leu Gly Ser Ile Phe
105 110 115
GAC CTG CTC GTG ATT GCT GCG GAC CGC TAC ATC ACC ATC TTC CAC GCA 1098
Asp Leu Leu Val Ile Ala Ala Asp Arg Tyr Ile Thr Ile Phe His Ala
120 125 130 135
CTG CGG TAC CAC AGC ATC GTG ACC ATG CGC CGC ACT GTG GTG GTG CTT 1146
Leu Arg Tyr His Ser Ile Val Thr Met Arg Arg Thr Val Val Val Leu
140 145 150
ACG GTC ATC TGG ACG TTC TGC ACG GGG ACT GGC ATC ACC ATG GTG ATC 1194
Thr Val Ile Trp Thr Phe Cys Thr Gly Thr Gly Ile Thr Met Val Ile
155 160 165
TTC TCC CAT CAT GTG CCC CAC GTG ATC ACC TTC ACG TCG CTG TTC CCG 1242
Phe Ser His His Val Pro His Val Ile Thr Phe Thr Ser Leu Phe Pro
170 175 180
CTG ATG CTG GTC TTC ATC CTG TGC CTC TAT GTG CAC ATG TTC CTG CTG 1290
Leu Met Leu Val Phe Ile Leu Cys Leu Tyr Val His Met Phe Leu Leu
185 190 195
GCT CGA TGG CAC ACC AGG AAG ATC TCC ACC CTC CCC AGA GCC AAC ATG 1338
Ala Arg Trp His Thr Arg Lys Ile Ser Thr Leu Pro Arg Ala Asn Met
200 205 210 215
AAA GGG GCC ATG ACA CTG ACC ATC CTG CTC GGG GTC TTC ATC TTC TGC 1386
Lys Gly Ala Met Thr Leu Thr Ile Leu Leu Gly Val Phe Ile Phe Cys
220 225 230
TGG GCC CCC TTT GTG CTT CAT GTC CTC TTG ATG ACA TTC TGC CCA AGT 1434
Trp Ala Pro Phe Val Leu His Val Leu Leu Met Thr Phe Cys Pro Ser
235 240 245
AAC CCC TAC TGC GCC TGC TAC ATG TCT CTC TTC CAG GTG AAC GGC ATG 1482
Asn Pro Tyr Cys Ala Cys Tyr Met Ser Leu Phe Gln Val Asn Gly Met
250 255 260
TTG ATC ATG TGC AAT GCC GTC ATT GAC CCC TTC ATA TAT GCC TTC CGG 1530
Leu Ile Met Cys Asn Ala Val Ile Asp Pro Phe Ile Tyr Ala Phe Arg
265 270 275
AGC CCA GAG CTC AGG GAC GCA TTC AAA AAG ATG ATC TTC TGC AGC AGG 1578
Ser Pro Glu Leu Arg Asp Ala Phe Lys Lys Met Ile Phe Cys Ser Arg
280 285 290 295
TAC TGG TAG AATGGCTGAT CCCTGGTTTT AGAATCCATG GGAATAACGT 1627
Tyr Trp *
TGCCAAGTGC CAGAATAGTG TAACATTCCA ACAAATGCCA GTGCTCCTCA CTGGCCTTCC 1687
TTCCCTAATG GATGCAAGGA TGACCCACCA GCTAGTGTTT CTGAATACTA TGGCCAGGAA 1747
CAGTCTATTG TAGGGGCAAC TCTATTTGTG ACTGGACAGA TAAAACGTGT AGTAAAAGAA 1807
GGATAGAATA CAAAGTATTA GGTACAAAAG TAATTAGGTT TGCATTACTT ATGACAAATG 1867
CATTACTTTT GCACCAATCT AGTAAAACAG CAATAAAAAT TCAAGGGCTT TGGGCTAAGG 1927
CAAAGACTTG CTTTCCTGTG GACATTAACA AGCCAGTTCT GAGGCGGCCT TTCCAGGTGG 1987
AGGCCATTGC AGCCAATTTC AGAGT 2012






297 amino acids


amino acid


linear




protein



8
Met Lys His Ile Ile Asn Ser Tyr Glu Asn Ile Asn Asn Thr Ala Arg
1 5 10 15
Asn Asn Ser Asp Cys Pro Arg Cys Val Leu Pro Glu Glu Ile Phe Phe
20 25 30
Thr Ile Ser Ile Val Gly Val Leu Glu Asn Leu Ile Val Leu Leu Ala
35 40 45
Val Phe Lys Asn Lys Asn Leu Gln Ala Pro Met Tyr Phe Phe Ile Cys
50 55 60
Ser Leu Ala Ile Ser Asp Met Leu Gly Ser Leu Tyr Lys Ile Leu Glu
65 70 75 80
Asn Ile Leu Ile Ile Leu Arg Asn Met Gly Ile Leu Lys Pro Arg Gly
85 90 95
Ser Phe Glu Thr Thr Ala His Asp Ile Ile Asp Ser Leu Phe Leu Leu
100 105 110
Ser Arg Leu Gly Ser Ile Phe Asp Leu Leu Val Ile Ala Ala Asp Arg
115 120 125
Tyr Ile Thr Ile Phe His Ala Leu Arg Tyr His Ser Ile Val Thr Met
130 135 140
Arg Arg Thr Val Val Val Leu Thr Val Ile Trp Thr Phe Cys Thr Gly
145 150 155 160
Thr Gly Ile Thr Met Val Ile Phe Ser His His Val Pro His Val Ile
165 170 175
Thr Phe Thr Ser Leu Phe Pro Leu Met Leu Val Phe Ile Leu Cys Leu
180 185 190
Tyr Val His Met Phe Leu Leu Ala Arg Trp His Thr Arg Lys Ile Ser
195 200 205
Thr Leu Pro Arg Ala Asn Met Lys Gly Ala Met Thr Leu Thr Ile Leu
210 215 220
Leu Gly Val Phe Ile Phe Cys Trp Ala Pro Phe Val Leu His Val Leu
225 230 235 240
Leu Met Thr Phe Cys Pro Ser Asn Pro Tyr Cys Ala Cys Tyr Met Ser
245 250 255
Leu Phe Gln Val Asn Gly Met Leu Ile Met Cys Asn Ala Val Ile Asp
260 265 270
Pro Phe Ile Tyr Ala Phe Arg Ser Pro Glu Leu Arg Asp Ala Phe Lys
275 280 285
Lys Met Ile Phe Cys Ser Arg Tyr Trp
290 295






1108 base pairs


nucleic acid


single


linear




cDNA to mRNA




5′UTR


1..132





CDS


133..1026





3′UTR


1027..1106




9
GGGGCCAGAA AGTTCCTGCT TCAGAGCAGA AGATCTTCAG CAAGAACTAC AAAGAAGAAA 60
AGATTCTGGA GAATCAATCA AGTTTCCTGT CAAGTTCCAG TAACGTTTCT GTCTTAACTG 120
CACACAGGAA AG ATG AAA CAC ATT CTC AAT CTG TAT GAA AAC CTC AAC 168
Met Lys His Ile Leu Asn Leu Tyr Glu Asn Leu Asn
1 5 10
AGT ACA GCA AGA AAT AAC TCA GAC TGT CCT GCT GTG ATT TTG CCA GAA 216
Ser Thr Ala Arg Asn Asn Ser Asp Cys Pro Ala Val Ile Leu Pro Glu
15 20 25
GAG ATA TTT TTC ACA GTA TCC ATT GTT GGG GTT TTG GAG AAC CTG ATG 264
Glu Ile Phe Phe Thr Val Ser Ile Val Gly Val Leu Glu Asn Leu Met
30 35 40
GTC CTT CTG GCT GTG GCC AAG AAT AAG ATG CTT CAG TCG CCC ATG TAC 312
Val Leu Leu Ala Val Ala Lys Asn Lys Met Leu Gln Ser Pro Met Tyr
45 50 55 60
TTT TTC ATC TGC AGC TTG GCT ATT TCC GAT ATG CTG GGG AGC ATG TAC 360
Phe Phe Ile Cys Ser Leu Ala Ile Ser Asp Met Leu Gly Ser Met Tyr
65 70 75
AAG ATT TTG GAA AAC GTT CTG ATC ATG TTC AAA AAC ATG GGT TAC CTC 408
Lys Ile Leu Glu Asn Val Leu Ile Met Phe Lys Asn Met Gly Tyr Leu
80 85 90
GAG CCT CGA GGC AGT TTT GAA AGC ACA GCA GAT GAT GTG GTG GAC TCC 456
Glu Pro Arg Gly Ser Phe Glu Ser Thr Ala Asp Asp Val Val Asp Ser
95 100 105
CTG TTC ATC CTC TCC CTT CTC GGC TCC ATC TGC AGC CTG TCT GTG ATT 504
Leu Phe Ile Leu Ser Leu Leu Gly Ser Ile Cys Ser Leu Ser Val Ile
110 115 120
GCC GCT GAC CGC TAC ACT ACA ATC TTC CAC GCT CTG CAG TAC CAC CGC 552
Ala Ala Asp Arg Tyr Thr Thr Ile Phe His Ala Leu Gln Tyr His Arg
125 130 135 140
ATC ATG ACC CCC GCA CCG TGC CCT CGT CAT CTG ACG GTC CTC TGG CGA 600
Ile Met Thr Pro Ala Pro Cys Pro Arg His Leu Thr Val Leu Trp Arg
145 150 155
GGC TGC ACA GGC AGT GGC ATT ACC ATC GTG ACC TTC TCC CAT CAC GTC 648
Gly Cys Thr Gly Ser Gly Ile Thr Ile Val Thr Phe Ser His His Val
160 165 170
CCC ACA GTG ATC GCC TTC ACA GCG CTG TTC CCG CTG ATG CTG GCC TTC 696
Pro Thr Val Ile Ala Phe Thr Ala Leu Phe Pro Leu Met Leu Ala Phe
175 180 185
ATC CTG TGC CTC TAC GTG CAC ATG TTC CTG CTG GCC CGC TCC CAC ACC 744
Ile Leu Cys Leu Tyr Val His Met Phe Leu Leu Ala Arg Ser His Thr
190 195 200
AGG AGG ACC CCC TCC CTT CCC AAA GCC AAC ATG AGA GGG GCC GTC ACA 792
Arg Arg Thr Pro Ser Leu Pro Lys Ala Asn Met Arg Gly Ala Val Thr
205 210 215 220
CTG ACT GTC CTG CTC GGG GTC TTC ATT TTC TGT TGG GCA CCC TTT GTC 840
Leu Thr Val Leu Leu Gly Val Phe Ile Phe Cys Trp Ala Pro Phe Val
225 230 235
CTT CAT GTC CTC TTG ATG ACA TTC TGC CCA GCT GAC CCC TAC TGT GCC 888
Leu His Val Leu Leu Met Thr Phe Cys Pro Ala Asp Pro Tyr Cys Ala
240 245 250
TGC TAC ATG TCC CTC TTC CAG GTG AAT GGT GTG TTG ATC ATG TGT AAT 936
Cys Tyr Met Ser Leu Phe Gln Val Asn Gly Val Leu Ile Met Cys Asn
255 260 265
GCC ATC ATC GAC CCC TTC ATA TAT GCC TTT CGG AGC CCA GAG CTC AGG 984
Ala Ile Ile Asp Pro Phe Ile Tyr Ala Phe Arg Ser Pro Glu Leu Arg
270 275 280
GTC GCA TTC AAA AAG ATG GTT ATC TGC AAC TGT TAC CAG TAG 1026
Val Ala Phe Lys Lys Met Val Ile Cys Asn Cys Tyr Gln *
285 290 295
AATGATTGGT CCCTGATTTT AGGAGCCACA GGGATATACT GTCAGGGACA GAGTAGCGTG 1086
ACAGACCAAC AACACTAGGA CT 1108






297 amino acids


amino acid


linear




protein



10
Met Lys His Ile Leu Asn Leu Tyr Glu Asn Leu Asn Ser Thr Ala Arg
1 5 10 15
Asn Asn Ser Asp Cys Pro Ala Val Ile Leu Pro Glu Glu Ile Phe Phe
20 25 30
Thr Val Ser Ile Val Gly Val Leu Glu Asn Leu Met Val Leu Leu Ala
35 40 45
Val Ala Lys Asn Lys Met Leu Gln Ser Pro Met Tyr Phe Phe Ile Cys
50 55 60
Ser Leu Ala Ile Ser Asp Met Leu Gly Ser Met Tyr Lys Ile Leu Glu
65 70 75 80
Asn Val Leu Ile Met Phe Lys Asn Met Gly Tyr Leu Glu Pro Arg Gly
85 90 95
Ser Phe Glu Ser Thr Ala Asp Asp Val Val Asp Ser Leu Phe Ile Leu
100 105 110
Ser Leu Leu Gly Ser Ile Cys Ser Leu Ser Val Ile Ala Ala Asp Arg
115 120 125
Tyr Thr Thr Ile Phe His Ala Leu Gln Tyr His Arg Ile Met Thr Pro
130 135 140
Ala Pro Cys Pro Arg His Leu Thr Val Leu Trp Arg Gly Cys Thr Gly
145 150 155 160
Ser Gly Ile Thr Ile Val Thr Phe Ser His His Val Pro Thr Val Ile
165 170 175
Ala Phe Thr Ala Leu Phe Pro Leu Met Leu Ala Phe Ile Leu Cys Leu
180 185 190
Tyr Val His Met Phe Leu Leu Ala Arg Ser His Thr Arg Arg Thr Pro
195 200 205
Ser Leu Pro Lys Ala Asn Met Arg Gly Ala Val Thr Leu Thr Val Leu
210 215 220
Leu Gly Val Phe Ile Phe Cys Trp Ala Pro Phe Val Leu His Val Leu
225 230 235 240
Leu Met Thr Phe Cys Pro Ala Asp Pro Tyr Cys Ala Cys Tyr Met Ser
245 250 255
Leu Phe Gln Val Asn Gly Val Leu Ile Met Cys Asn Ala Ile Ile Asp
260 265 270
Pro Phe Ile Tyr Ala Phe Arg Ser Pro Glu Leu Arg Val Ala Phe Lys
275 280 285
Lys Met Val Ile Cys Asn Cys Tyr Gln
290 295






1338 base pairs


nucleic acid


single


linear




cDNA to mRNA




5′UTR


1..297





CDS


298..1269





3′UTR


1270..1338




11
GGCTGTAACT GTAGCAACCG GTGTTGGGTG GGGATGAGAA GAGACCAGAG AGAGAGAGGG 60
TCAGAGCGAC AGGGGATGAG ACAGGCTGGT CAGAGTCTGC ACTGATTGTT GGAGACGCAA 120
AGGAAAGTTT TTTCTATGTC TCCAACCTCC CCCTCCTCCC CCGTTTCTCT CTGGAGAAAC 180
TAAAATGTAG ACTGGACAGC ATCCACAAGA GAAGCACCTA GAAGAAGATT TTTTTTTCCC 240
AGCAGCTTGC TCAGGACCCT GCAGGAGCTG CAGCCGGAAC TGGTCCCGCC GATAACC 297
ATG AAC TCT TCC TGC TGC CCG TCC TCC TCT TAT CCG ACG CTG CCT AAC 345
Met Asn Ser Ser Cys Cys Pro Ser Ser Ser Tyr Pro Thr Leu Pro Asn
1 5 10 15
CTC TCC CAG CAC CCT GCA GCC CCC TCT GCC AGC AAC CGG AGT GGC AGT 393
Leu Ser Gln His Pro Ala Ala Pro Ser Ala Ser Asn Arg Ser Gly Ser
20 25 30
GGG TTC TGC GAG CAG GTT TTC ATC AAG CCA GAG GTC TTC CTG GCA CTG 441
Gly Phe Cys Glu Gln Val Phe Ile Lys Pro Glu Val Phe Leu Ala Leu
35 40 45
GGC ATC GTC AGT CTG ATG GAA AAC ATC CTG GTG ATC CTG GCT GTG GTG 489
Gly Ile Val Ser Leu Met Glu Asn Ile Leu Val Ile Leu Ala Val Val
50 55 60
AGG AAC GGC AAC CTG CAC TCC CCC ATG TAC TTC TTC CTG CTG AGC CTG 537
Arg Asn Gly Asn Leu His Ser Pro Met Tyr Phe Phe Leu Leu Ser Leu
65 70 75 80
CTG CAG GCC GAC CTG CTG GTG AGC CTG TCC AAC TCC CTG GAG ACC ATC 585
Leu Gln Ala Asp Leu Leu Val Ser Leu Ser Asn Ser Leu Glu Thr Ile
85 90 95
ATG ATC GTG GTT ATC AAC AGC GAC TCC CTG ACC TTG GAG GAC CAA TTC 633
Met Ile Val Val Ile Asn Ser Asp Ser Leu Thr Leu Glu Asp Gln Phe
100 105 110
ATC CAG CAC ATG GAC AAC ATC TTC GAC TCT ATG ATC TGC ATC TCC CTG 681
Ile Gln His Met Asp Asn Ile Phe Asp Ser Met Ile Cys Ile Ser Leu
115 120 125
GTG GCC TCC ATC TGC AAC CTC CTG GCC ATC GCC GTG GAC AGG TAC GTC 729
Val Ala Ser Ile Cys Asn Leu Leu Ala Ile Ala Val Asp Arg Tyr Val
130 135 140
ACC ATC TTC TAT GCC CTC CGT TAC CAC AGC ATC ATG ACG GTT AGG AAA 777
Thr Ile Phe Tyr Ala Leu Arg Tyr His Ser Ile Met Thr Val Arg Lys
145 150 155 160
GCC CTC TCC TTG ATC GTG GCC ATC TGG GTC TGC TGT GGC ATC TGC GGC 825
Ala Leu Ser Leu Ile Val Ala Ile Trp Val Cys Cys Gly Ile Cys Gly
165 170 175
GTG ATG TTC ATC GTC TAC TCC GAG AGC AAG ATG GTC ATC GTG TGC CTC 873
Val Met Phe Ile Val Tyr Ser Glu Ser Lys Met Val Ile Val Cys Leu
180 185 190
ATC ACC ATG TTC TTC GCC ATG GTG CTC CTC ATG GGC ACC CTG TAC ATC 921
Ile Thr Met Phe Phe Ala Met Val Leu Leu Met Gly Thr Leu Tyr Ile
195 200 205
CAC ATG TTC CTC TTC GCC AGG CTG CAC GTC CAG CGC ATC GCG GCA CTG 969
His Met Phe Leu Phe Ala Arg Leu His Val Gln Arg Ile Ala Ala Leu
210 215 220
CCA CCT GCT GAC GGG CTA GCC CCG CAG CAG CAC TCG TGC ATG AAG GGG 1017
Pro Pro Ala Asp Gly Leu Ala Pro Gln Gln His Ser Cys Met Lys Gly
225 230 235 240
GCC GTC ACC ATC ACC ATC CTG CTG GGG GTT TTC ATC TTC TGC TGG GCG 1065
Ala Val Thr Ile Thr Ile Leu Leu Gly Val Phe Ile Phe Cys Trp Ala
245 250 255
CCT TTC TTC CTC CAC CTG GTC CTC ATC ATC ACC TGC CCC ACC AAC CCC 1113
Pro Phe Phe Leu His Leu Val Leu Ile Ile Thr Cys Pro Thr Asn Pro
260 265 270
TAC TGC ATC TGC TAC ACG GCG CAC TTC AAC ACC TAC CTG GTT CTC ATC 1161
Tyr Cys Ile Cys Tyr Thr Ala His Phe Asn Thr Tyr Leu Val Leu Ile
275 280 285
ATG TGC AAC TCT GTC ATC GAC CCC CTC ATC TAC GCC TTC CGC AGC CTG 1209
Met Cys Asn Ser Val Ile Asp Pro Leu Ile Tyr Ala Phe Arg Ser Leu
290 295 300
GAG CTG CGA AAC ACC TTC AAG GAG ATT CTC TGC GGT TGC AAT GGC ATG 1257
Glu Leu Arg Asn Thr Phe Lys Glu Ile Leu Cys Gly Cys Asn Gly Met
305 310 315 320
AAC GTG GGC TAG GAACCCCCGA GGAGGTGTTC CACGGCTAGC CAAGAGAGAA 1309
Asn Val Gly *
AAGCAATGCT CAGGTGAGAC ACAGAAGGG 1338






323 amino acids


amino acid


linear




protein



12
Met Asn Ser Ser Cys Cys Pro Ser Ser Ser Tyr Pro Thr Leu Pro Asn
1 5 10 15
Leu Ser Gln His Pro Ala Ala Pro Ser Ala Ser Asn Arg Ser Gly Ser
20 25 30
Gly Phe Cys Glu Gln Val Phe Ile Lys Pro Glu Val Phe Leu Ala Leu
35 40 45
Gly Ile Val Ser Leu Met Glu Asn Ile Leu Val Ile Leu Ala Val Val
50 55 60
Arg Asn Gly Asn Leu His Ser Pro Met Tyr Phe Phe Leu Leu Ser Leu
65 70 75 80
Leu Gln Ala Asp Leu Leu Val Ser Leu Ser Asn Ser Leu Glu Thr Ile
85 90 95
Met Ile Val Val Ile Asn Ser Asp Ser Leu Thr Leu Glu Asp Gln Phe
100 105 110
Ile Gln His Met Asp Asn Ile Phe Asp Ser Met Ile Cys Ile Ser Leu
115 120 125
Val Ala Ser Ile Cys Asn Leu Leu Ala Ile Ala Val Asp Arg Tyr Val
130 135 140
Thr Ile Phe Tyr Ala Leu Arg Tyr His Ser Ile Met Thr Val Arg Lys
145 150 155 160
Ala Leu Ser Leu Ile Val Ala Ile Trp Val Cys Cys Gly Ile Cys Gly
165 170 175
Val Met Phe Ile Val Tyr Ser Glu Ser Lys Met Val Ile Val Cys Leu
180 185 190
Ile Thr Met Phe Phe Ala Met Val Leu Leu Met Gly Thr Leu Tyr Ile
195 200 205
His Met Phe Leu Phe Ala Arg Leu His Val Gln Arg Ile Ala Ala Leu
210 215 220
Pro Pro Ala Asp Gly Leu Ala Pro Gln Gln His Ser Cys Met Lys Gly
225 230 235 240
Ala Val Thr Ile Thr Ile Leu Leu Gly Val Phe Ile Phe Cys Trp Ala
245 250 255
Pro Phe Phe Leu His Leu Val Leu Ile Ile Thr Cys Pro Thr Asn Pro
260 265 270
Tyr Cys Ile Cys Tyr Thr Ala His Phe Asn Thr Tyr Leu Val Leu Ile
275 280 285
Met Cys Asn Ser Val Ile Asp Pro Leu Ile Tyr Ala Phe Arg Ser Leu
290 295 300
Glu Leu Arg Asn Thr Phe Lys Glu Ile Leu Cys Gly Cys Asn Gly Met
305 310 315 320
Asn Val Gly






30 base pairs


nucleic acid


single


linear




DNA (genomic)




misc_feature


1..30



/function = “Degenerate
oligonucleotide primer (sense)”





13
GAGTCGACCR CCCATGTAYT DYTTCATCTG 30






30 base pairs


nucleic acid


single


linear




DNA (genomic)




misc_feature


1..30



/function = “Degenerate
oligonucleotide primer (sense)”





14
CAGAATTCGG AARGCRTAKA TGARGGGGTC 30






1671 base pairs


nucleic acid


single


linear




cDNA to mRNA




5′UTR


1..393





CDS


394..1389





3′UTR


1390..1671




15
AGCTTCCGAG AGGCAGCCGA TGTGAGCATG TGCGCACAGA TTCGTCTCCC AATGGCATGG 60
CAGCTTCAAG GAAAATTATT TTGAACAGAC TTGAATGCAT AAGATTAAAG TTAAAGCAGA 120
AGTGAGAACA AGAAAGCAAA GAGCAGACTC TTTCAACTGA GAATGAATAT TTTGAAGCCC 180
AAGATTTTAA CGTGATGATG ATTAGAGTCG TACCTAAAAG AGACTAAAAA CTCCATGTCA 240
AGCTCTGGAC TTGTGACATT TACTCACAGC AGGCATGGCA ATTTTAGCCT CACAACTTTC 300
AGACAGATAA AGACTTGGAG GAAATAACTG AGACGACTCC CTGACCCAGG AGGTTAAATC 360
AATTCAGGGG GACACTGGAA TTCTCCTGCC AGC ATG GTG AAC TCC ACC CAC CGT 414
Met Val Asn Ser Thr His Arg
1 5
GGG ATG CAC ACT TCT CTG CAC CTC TGG AAC CGC AGC AGT TAC AGA CTG 462
Gly Met His Thr Ser Leu His Leu Trp Asn Arg Ser Ser Tyr Arg Leu
10 15 20
CAC AGC AAT GCC AGT GAG TCC CTT GGA AAA GGC TAC TCT GAT GGA GGG 510
His Ser Asn Ala Ser Glu Ser Leu Gly Lys Gly Tyr Ser Asp Gly Gly
25 30 35
TGC TAC GCG CAA CTT TTT GTC TCT CCT GAG GTG TTT GTG ACT CTG GGT 558
Cys Tyr Ala Gln Leu Phe Val Ser Pro Glu Val Phe Val Thr Leu Gly
40 45 50 55
GTG ATC AGC TTG TTG GAG AAT ATC TTA GAG ATT GTG GCA ATA GCC AAG 606
Val Ile Ser Leu Leu Glu Asn Ile Leu Glu Ile Val Ala Ile Ala Lys
60 65 70
AAC AAG AAT CTG CAT TCA CCC ATG TAC TTT TTC ATC TGC AGC TTG GCT 654
Asn Lys Asn Leu His Ser Pro Met Tyr Phe Phe Ile Cys Ser Leu Ala
75 80 85
GTG GCT GAT ATG CTG GTG AGC GTT TCA AAT GGA TCA GAA ACC ATT ATC 702
Val Ala Asp Met Leu Val Ser Val Ser Asn Gly Ser Glu Thr Ile Ile
90 95 100
ATC ACC CTA TTA AAC CGT ACA GAT ACG GAT GCA CAG AGT TTC ACA GTG 750
Ile Thr Leu Leu Asn Arg Thr Asp Thr Asp Ala Gln Ser Phe Thr Val
105 110 115
AAT ATT GAT AAT GTC ATT GAC TCG GTG ATC TGT AGC TCC TTG CTT GCA 798
Asn Ile Asp Asn Val Ile Asp Ser Val Ile Cys Ser Ser Leu Leu Ala
120 125 130 135
TCC ATT TGC AGC CTG CTT TCA ATT GCA GTG GAC AGG TAC TTT ACT ATC 846
Ser Ile Cys Ser Leu Leu Ser Ile Ala Val Asp Arg Tyr Phe Thr Ile
140 145 150
TTC TAT GCT CTC CAG TAC CAT AAC ATT ATG ACA GTT AAG CGG GTT GGG 894
Phe Tyr Ala Leu Gln Tyr His Asn Ile Met Thr Val Lys Arg Val Gly
155 160 165
ATC AGC ATA AGT TGT ATC TGG GCA GCT TGC ACG GTT TCA GGT ATT TTG 942
Ile Ser Ile Ser Cys Ile Trp Ala Ala Cys Thr Val Ser Gly Ile Leu
170 175 180
TTC ATC ATT TAC TCA GAT AGT AGT GCT GTC ATC ATC TGC CTC ATC ACC 990
Phe Ile Ile Tyr Ser Asp Ser Ser Ala Val Ile Ile Cys Leu Ile Thr
185 190 195
ATG TTC TTC ACC ATG CTG GCT CTC ATG GCT TCT CTC TAT GTC CAC CTG 1038
Met Phe Phe Thr Met Leu Ala Leu Met Ala Ser Leu Tyr Val His Leu
200 205 210 215
TTC CTG ATG GCC AGG CTT CAC ATT AAG AGG ATT GCT GTC CTC CCC GGC 1086
Phe Leu Met Ala Arg Leu His Ile Lys Arg Ile Ala Val Leu Pro Gly
220 225 230
ACT GGT GCC ATC CGC CAA GGT GCC AAT ATG AAG GGA GCG ATT ACC TTG 1134
Thr Gly Ala Ile Arg Gln Gly Ala Asn Met Lys Gly Ala Ile Thr Leu
235 240 245
ACC ATC CTG ATT GGC GTC TTT GTT GTC TGC TGG GCC CCA TTC TTC CTC 1182
Thr Ile Leu Ile Gly Val Phe Val Val Cys Trp Ala Pro Phe Phe Leu
250 255 260
CAC TTA ATA TTC TAC ATC TCT TGT CCT CAG AAT CCA TAT TGT GTG TGC 1230
His Leu Ile Phe Tyr Ile Ser Cys Pro Gln Asn Pro Tyr Cys Val Cys
265 270 275
TTC ATG TCT CAC TTT AAC TTG TAT CTC ATA CTG ATC ATG TGT AAT TCA 1278
Phe Met Ser His Phe Asn Leu Tyr Leu Ile Leu Ile Met Cys Asn Ser
280 285 290 295
ATC ATC GAT CCT CTG ATT TAT GCA CTC CGG AGT CAA GAA CTG AGG AAA 1326
Ile Ile Asp Pro Leu Ile Tyr Ala Leu Arg Ser Gln Glu Leu Arg Lys
300 305 310
ACC TTC AAA GAG ATC ATC TCT TCC TAT CCC CTG GGA GGC CTT TGT GAC 1374
Thr Phe Lys Glu Ile Ile Ser Ser Tyr Pro Leu Gly Gly Leu Cys Asp
315 320 325
TTG TCT AGC AGA TAT TAAATGGGGA CAGAGCACGC AATATAGGAA CATCCATAAG 1429
Leu Ser Ser Arg Tyr
330
AGACTTTTTC ACTCTTACCC TACCTGAATA TTCTACTTCT GCAACAGCTT TCTCTTCCGT 1489
GTAGGGTACT GGTTGAGATA TCCATTGTGT AAATTTAAGC CTATGATTTT TAATGAGAAA 1549
AAATGCCCAG TCTCTGTATT ATTTCCAATC TCATGCTACT TTTTTGGCCA TAAAATATGA 1609
ATCTATGTTA TAGGTTGTAG GCACTGTGGA TTTACAAAAA GAAAAGTCCT TATTAAAAGC 1669
TT 1671






332 amino acids


amino acid


linear




protein



16
Met Val Asn Ser Thr His Arg Gly Met His Thr Ser Leu His Leu Trp
1 5 10 15
Asn Arg Ser Ser Tyr Arg Leu His Ser Asn Ala Ser Glu Ser Leu Gly
20 25 30
Lys Gly Tyr Ser Asp Gly Gly Cys Tyr Ala Gln Leu Phe Val Ser Pro
35 40 45
Glu Val Phe Val Thr Leu Gly Val Ile Ser Leu Leu Glu Asn Ile Leu
50 55 60
Glu Ile Val Ala Ile Ala Lys Asn Lys Asn Leu His Ser Pro Met Tyr
65 70 75 80
Phe Phe Ile Cys Ser Leu Ala Val Ala Asp Met Leu Val Ser Val Ser
85 90 95
Asn Gly Ser Glu Thr Ile Ile Ile Thr Leu Leu Asn Arg Thr Asp Thr
100 105 110
Asp Ala Gln Ser Phe Thr Val Asn Ile Asp Asn Val Ile Asp Ser Val
115 120 125
Ile Cys Ser Ser Leu Leu Ala Ser Ile Cys Ser Leu Leu Ser Ile Ala
130 135 140
Val Asp Arg Tyr Phe Thr Ile Phe Tyr Ala Leu Gln Tyr His Asn Ile
145 150 155 160
Met Thr Val Lys Arg Val Gly Ile Ser Ile Ser Cys Ile Trp Ala Ala
165 170 175
Cys Thr Val Ser Gly Ile Leu Phe Ile Ile Tyr Ser Asp Ser Ser Ala
180 185 190
Val Ile Ile Cys Leu Ile Thr Met Phe Phe Thr Met Leu Ala Leu Met
195 200 205
Ala Ser Leu Tyr Val His Leu Phe Leu Met Ala Arg Leu His Ile Lys
210 215 220
Arg Ile Ala Val Leu Pro Gly Thr Gly Ala Ile Arg Gln Gly Ala Asn
225 230 235 240
Met Lys Gly Ala Ile Thr Leu Thr Ile Leu Ile Gly Val Phe Val Val
245 250 255
Cys Trp Ala Pro Phe Phe Leu His Leu Ile Phe Tyr Ile Ser Cys Pro
260 265 270
Gln Asn Pro Tyr Cys Val Cys Phe Met Ser His Phe Asn Leu Tyr Leu
275 280 285
Ile Leu Ile Met Cys Asn Ser Ile Ile Asp Pro Leu Ile Tyr Ala Leu
290 295 300
Arg Ser Gln Glu Leu Arg Lys Thr Phe Lys Glu Ile Ile Ser Ser Tyr
305 310 315 320
Pro Leu Gly Gly Leu Cys Asp Leu Ser Ser Arg Tyr
325 330






978 base pairs


nucleic acid


single


linear




DNA (genomic)




CDS


1..975




17
ATG AAC TCC TCC TCC ACC CTG ACT GTA TTG AAT CTT ACC CTG AAC GCC 48
Met Asn Ser Ser Ser Thr Leu Thr Val Leu Asn Leu Thr Leu Asn Ala
1 5 10 15
TCA GAG GAT GGC ATT TTA GGA TCA AAT GTC AAG AAC AAG TCT TTG GCC 96
Ser Glu Asp Gly Ile Leu Gly Ser Asn Val Lys Asn Lys Ser Leu Ala
20 25 30
TGT GAA GAA ATG GGC ATT GCC GTG GAG GTG TTC CTG ACC CTG GGT CTC 144
Cys Glu Glu Met Gly Ile Ala Val Glu Val Phe Leu Thr Leu Gly Leu
35 40 45
GTC AGC CTC TTA GAG AAC ATC CTG GTC ATT GGG GCC ATA GTA AAG AAC 192
Val Ser Leu Leu Glu Asn Ile Leu Val Ile Gly Ala Ile Val Lys Asn
50 55 60
AAA AAC CTG CAC TCA CCC ATG TAC TTC TTT GTG GGC AGC TTA GCC GTG 240
Lys Asn Leu His Ser Pro Met Tyr Phe Phe Val Gly Ser Leu Ala Val
65 70 75 80
GCC GAC ATG CTG GTG AGC ATG TCC AAT GCC TGG GAG ACT GTC ACC ATA 288
Ala Asp Met Leu Val Ser Met Ser Asn Ala Trp Glu Thr Val Thr Ile
85 90 95
TAC TTG CTA AAT AAT AAA CAC CTG GTG ATA GCC GAC ACC TTT GTG CGA 336
Tyr Leu Leu Asn Asn Lys His Leu Val Ile Ala Asp Thr Phe Val Arg
100 105 110
CAC ATC GAC AAC GTG TTC GAC TCC ATG ATC TGC ATC TCT GTG GTG GCC 384
His Ile Asp Asn Val Phe Asp Ser Met Ile Cys Ile Ser Val Val Ala
115 120 125
TCG ATG TGC AGT TTG CTG GCC ATT GCG GTG GAT AGG TAC ATC ACC ATC 432
Ser Met Cys Ser Leu Leu Ala Ile Ala Val Asp Arg Tyr Ile Thr Ile
130 135 140
TTC TAT GCC TTG CGC TAC CAC CAC ATC ATG ACC GCG AGG CGC TCG GGG 480
Phe Tyr Ala Leu Arg Tyr His His Ile Met Thr Ala Arg Arg Ser Gly
145 150 155 160
GTG ATC ATC GCC TGC ATT TGG ACC TTC TGC ATA AGC TGC GGC ATT GTT 528
Val Ile Ile Ala Cys Ile Trp Thr Phe Cys Ile Ser Cys Gly Ile Val
165 170 175
TTC ATC ATC TAC TAT GAG TCC AAG TAT GTG ATC ATT TGC CTC ATC TCC 576
Phe Ile Ile Tyr Tyr Glu Ser Lys Tyr Val Ile Ile Cys Leu Ile Ser
180 185 190
ATG TTC TTC ACC ATG CTG TTC TTC ATG GTG TCT CTG TAT ATA CAC ATG 624
Met Phe Phe Thr Met Leu Phe Phe Met Val Ser Leu Tyr Ile His Met
195 200 205
TTC CTC CTG GCC CGG AAC CAT GTC AAG CGG ATA GCA GCT TCC CCC AGA 672
Phe Leu Leu Ala Arg Asn His Val Lys Arg Ile Ala Ala Ser Pro Arg
210 215 220
TAC AAC TCC GTG AGG CAA AGG ACC AGC ATG AAG GGG GCT ATT ACC CTC 720
Tyr Asn Ser Val Arg Gln Arg Thr Ser Met Lys Gly Ala Ile Thr Leu
225 230 235 240
ACC ATG CTA CTG GGG ATT TTC ATT GTC TGC TGG TCT CCC TTC TTT CTT 768
Thr Met Leu Leu Gly Ile Phe Ile Val Cys Trp Ser Pro Phe Phe Leu
245 250 255
CAC CTT ATC TTA ATG ATC TCC TGC CCT CAG AAC GTC TAC TGC TCT TGC 816
His Leu Ile Leu Met Ile Ser Cys Pro Gln Asn Val Tyr Cys Ser Cys
260 265 270
TTT ATG TCT TAC TTC AAC ATG TAC CTT ATA CTC ATC ATG TGC AAC TCC 864
Phe Met Ser Tyr Phe Asn Met Tyr Leu Ile Leu Ile Met Cys Asn Ser
275 280 285
GTG ATC GAT CCT CTC ATC TAC GCC CTC CGC AGC CAA GAG ATG CGG AGG 912
Val Ile Asp Pro Leu Ile Tyr Ala Leu Arg Ser Gln Glu Met Arg Arg
290 295 300
ACC TTT AAG GAG ATC GTC TGT TGT CAC GGA TTC CGG CGA CCT TGT AGG 960
Thr Phe Lys Glu Ile Val Cys Cys His Gly Phe Arg Arg Pro Cys Arg
305 310 315 320
CTC CTT GGC GGG TAT TAA 978
Leu Leu Gly Gly Tyr
325






325 amino acids


amino acid


linear




protein



18
Met Asn Ser Ser Ser Thr Leu Thr Val Leu Asn Leu Thr Leu Asn Ala
1 5 10 15
Ser Glu Asp Gly Ile Leu Gly Ser Asn Val Lys Asn Lys Ser Leu Ala
20 25 30
Cys Glu Glu Met Gly Ile Ala Val Glu Val Phe Leu Thr Leu Gly Leu
35 40 45
Val Ser Leu Leu Glu Asn Ile Leu Val Ile Gly Ala Ile Val Lys Asn
50 55 60
Lys Asn Leu His Ser Pro Met Tyr Phe Phe Val Gly Ser Leu Ala Val
65 70 75 80
Ala Asp Met Leu Val Ser Met Ser Asn Ala Trp Glu Thr Val Thr Ile
85 90 95
Tyr Leu Leu Asn Asn Lys His Leu Val Ile Ala Asp Thr Phe Val Arg
100 105 110
His Ile Asp Asn Val Phe Asp Ser Met Ile Cys Ile Ser Val Val Ala
115 120 125
Ser Met Cys Ser Leu Leu Ala Ile Ala Val Asp Arg Tyr Ile Thr Ile
130 135 140
Phe Tyr Ala Leu Arg Tyr His His Ile Met Thr Ala Arg Arg Ser Gly
145 150 155 160
Val Ile Ile Ala Cys Ile Trp Thr Phe Cys Ile Ser Cys Gly Ile Val
165 170 175
Phe Ile Ile Tyr Tyr Glu Ser Lys Tyr Val Ile Ile Cys Leu Ile Ser
180 185 190
Met Phe Phe Thr Met Leu Phe Phe Met Val Ser Leu Tyr Ile His Met
195 200 205
Phe Leu Leu Ala Arg Asn His Val Lys Arg Ile Ala Ala Ser Pro Arg
210 215 220
Tyr Asn Ser Val Arg Gln Arg Thr Ser Met Lys Gly Ala Ile Thr Leu
225 230 235 240
Thr Met Leu Leu Gly Ile Phe Ile Val Cys Trp Ser Pro Phe Phe Leu
245 250 255
His Leu Ile Leu Met Ile Ser Cys Pro Gln Asn Val Tyr Cys Ser Cys
260 265 270
Phe Met Ser Tyr Phe Asn Met Tyr Leu Ile Leu Ile Met Cys Asn Ser
275 280 285
Val Ile Asp Pro Leu Ile Tyr Ala Leu Arg Ser Gln Glu Met Arg Arg
290 295 300
Thr Phe Lys Glu Ile Val Cys Cys His Gly Phe Arg Arg Pro Cys Arg
305 310 315 320
Leu Leu Gly Gly Tyr
325






30 base pairs


nucleic acid


single


linear




DNA (genomic)




misc_feature


1..32



/function = “Degenerate
oligonucleotide primer (antisense)”





19
GAATTCGACG TCACAGTATG ACGGCCATGG 30







Claims
  • 1. A compound having the general formula:A-B-C-D-E-F-G-amide wherein A is Leu, Ile, or Met;B is Asp or Glu; C is His; D is D-Phe, D-Tyr or D-Nal; E is Arg, Lys, homoArg or homoLys; F is Trp; G is Lys or homoLys; wherein the peptide is cyclized by the formation of an amide bond between the side chain carboxyl group of the Asp or Glu residue at position B in the peptide, and the side chain amino group of the Lys or homoLys residue at position G, and wherein the compound is an agonist of MC-3 melanocortin receptor.
Parent Case Info

This application is a divisional of U.S. patent application, Ser. No. 08/706,281, filed Sep. 4, 1996 now U.S. Pat. No. 6,100,048, which is a CIP of Ser. No. 08/466,906, filed Jun. 6, 1993 (now U.S. Pat. No. 5,849,871); which is a divisional of Ser. No. 07/866,979 filed Apr. 10, 1992 (now U.S. Pat. No. 5,537,347) and which is a C-I-P of Ser. No. 08/478,992 filed Jun. 7, 1995 (now U.S. Pat. No. 5,773,229) which is a divisional of Ser. No. 08/077,675 filed Jun. 15, 1993 (now U.S. Pat. No. 5,554,729) which is a divisional of Ser. No. 07/866,560 filed Apr. 10, 1992 (now U.S. Pat. No. 5,280,112) and which is a C-I-P of Ser. No. 08/044,812 filed Apr. 8, 1993 (now U.S. Pat. No. 5,837,521).

Government Interests

This invention was made with government support under R29DK41921, R01DK43859, P01DK44239, AR42415 and HD30236 by the National Institutes of Health. The government has certain rights in the invention.

US Referenced Citations (5)
Number Name Date Kind
5554729 Cone et al. Sep 1996 A
5674839 Hruley et al. Oct 1997 A
5773229 Cone et al. Jun 1998 A
5837521 Cone et al. Nov 1998 A
5849871 Cone et al. Dec 1998 A
Continuation in Parts (3)
Number Date Country
Parent 08/466906 Jun 1995 US
Child 08/706281 US
Parent 08/478992 Jun 1995 US
Child 07/866979 US
Parent 08/044812 Apr 1993 US
Child 07/866560 US