Claims
- 1. A method of determining the presence of an AL-1 nucleic acid sequence in a sample, comprising:
- (a) contacting a sample nucleic acid with a nucleic acid molecule which has at least 10 contiguous nucleotides selected from (i) nucleotides 323 to 1006 of SEQ ID NO: 3, (ii) the codons of a nucleic acid sequence encoding the amino acid sequence SEQ ID NO: 4, or (iii) an exact complementary nucleic acid sequence of (i) or (ii), and which hybridizes to the nucleic acid sequence of (i), (ii) or (iii);
- (b) hybridizing the nucleic acid molecule to the sample nucleic acid to create a hybrid molecule; and
- (c) determining the presence of AL-1 nucleic acid in the sample by means of the hybrid molecule.
- 2. The method of claim 1, wherein the nucleic acid molecule consists of at least 10 contiguous nucleotides selected from (i) nucleotides 323 to 1006, inclusive, of SEQ ID NO: 3, (ii) the codons of a nucleic acid sequence encoding the amino acid sequence SEQ ID NO: 4, or (iii) an exact complementary nucleic acid sequence of (i) or (ii), and which hybridizes to the nucleic acid sequence of (i), (ii) or (iii).
- 3. The method of claim 1, wherein the nucleic acid molecule has at least 10 contiguous nucleotides selected from nucleotides 323 to 1006 inclusive, of SEQ ID NO: 3 or an exact complementary sequence thereof.
- 4. The method of claim 1, wherein the nucleic acid molecule consists essentially of at least 10 contiguous nucleotides selected from (i) nucleotides 323 to 1006, inclusive, of SEQ ID NO: 3, (ii) the codons of a nucleic acid sequence encoding the amino acid sequence SEO ID NO: 4, or (iii) an exact complementary nucleic acid sequence of (i) or (ii), and which hybridizes to the nucleic acid sequence of (i), (ii) or (iii).
- 5. The method of claim 1, wherein the nucleic acid molecule has at least 15 nucleotides selected from (i), (ii) or (iii).
- 6. The method of claim 5, wherein the nucleic acid molecule has more than 25 nucleotides selected from (i), (ii) or (iii).
- 7. A method of amplifying a target AL-1 nucleic acid in a sample, comprising:
- (a) contacting the sample nucleic acid with a primer having at least 10 contiguous nucleotides selected from (i) nucleotides 323 to 1006 of SEQ ID NO: 3, (ii) the codons of a nucleic acid sequence encoding the amino acid sequence SEQ ID NO: 4, or (iii) an exact complementary nucleic acid sequence of (i) or (ii);
- (b) hybridizing the primer to the nucleic acid sample; and,
- (c) performing an amplification reaction to amplify preferentially target AL-1 nucleic acid sequence within the sample.
- 8. The method of claim 7, wherein the primer hybridizes to (i), (ii), or (iii).
- 9. The method of claim 7, wherein the primer has at least 10 contiguous nucleotides selected from nucleotides 323 to 1006, inclusive of SEQ ID NO: 3 or an exact complementary sequence thereof.
- 10. The method of claim 9, wherein the primer consists essentially of at least 10 contiguous nucleotides selected from (i) nucleotides 323 to 1006, inclusive, of SEQ ID NO: 3, (ii) the codons of a nucleic acid sequence encoding the amino acid sequence SEQ ID NO: 4, or (iii) an exact complementary nucleic acid sequence of (i) or (ii), and which hybridizes to the nucleic acid sequence of (i), (ii) or (iii).
- 11. The method of claim 7, wherein the primer has at least 15 nucleotides selected from (i), (ii) or (iii).
- 12. The method of claim 11, wherein the primer has more than 25 nucleotides selected from (i), (ii) or (iii).
- 13. The method of claim 7, which further comprises the steps of:
- (a) contacting the sample nucleic acid with a second primer having at least 10 contiguous nucleotides selected from (i) nucleotides 323 to 1006 of SEQ ID NO: 3, (ii) the codons of a nucleic acid sequence encoding the amino acid sequence SEQ ID NO: 4, or (iii) an exact complementary nucleic acid sequence of (i) or (ii); and
- (b) hybridizing the second primer to the sample nucleic acid.
- 14. The method of claim 13, wherein the second primer hybridizes to (i), (ii), or (iii).
- 15. The method of claim 13, wherein the second primer has at least 10 contiguous nucleotides selected from nucleotides 323 to 1006 of SEQ ID NO: 3 or an exact complementary sequence thereof.
- 16. The method of claim 15, wherein the second primer consists essentially of at least 10 contiguous nucleotides selected from (i) nucleotides 323 to 1006, inclusive, of SEQ ID NO: 3, (ii) the codons of a nucleic acid sequence encoding the amino acid sequence SEQ ID NO: 4, or (iii) an exact complementary nucleic acid sequence of (i) or (ii), and which hybridizes to the nucleic acid sequence of (i), (ii) or (iii).
- 17. The method of claim 13, wherein the second primer has at least 15 nucleotides selected from (i), (ii) or (iii).
- 18. The method of claim 17, wherein the second primer has more than 25 nucleotides selected from (i), (ii) or (iii).
Parent Case Info
This is a continuation of co-pending application Ser. No. 08/330,128 filed on Oct. 27, 1994, which application is incorporated herein by reference and to which application priority is claimed under 35 USC .sctn. 120.
Foreign Referenced Citations (1)
Number |
Date |
Country |
597503 A2 |
May 1994 |
EPX |
Continuations (1)
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Number |
Date |
Country |
Parent |
330128 |
Oct 1994 |
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