Claims
- 1. A method of delivering a heterologous gene to a periosteal cell of a mammal, comprising:
a) providing recombinant adeno-associated virus (rAAV) virions, wherein said rAAV virions comprise said heterologous gene; b) contacting said periosteal cell with said rAAV virions, wherein said contacting results in transduction of said periosteal cell by said rAAV virions; and c) expressing said heterologous gene in said periosteal cell.
- 2. A method of expressing a heterologous gene in a mammal, comprising:
(a) providing recombinant adeno-associated virus (rAAV) virions, wherein said rAAV virions comprise said heterologous gene; (b) contacting a periosteal cell with said rAAV virions, wherein said contacting results in transduction of said periosteal cell by said rAAV virions; (c) delivering said periosteal cell to said mammal; and (d) expressing said heterologous gene.
- 3. The method of claim 2, wherein said periosteal cell is contained within a matrix.
- 4. The method of claim 3, wherein said matrix is composed of collagen.
- 5. The method of claim 4, wherein said collagen is type II collagen.
- 6. The method of claim 2, wherein said mammal has a skeletal disorder.
- 7. The method of claim 6, wherein said skeletal disorder is an articular cartilage defect.
- 8. The method of claim 7, wherein said articular cartilage defect is osteoarthritis.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is related to provisional patent application Ser. No. 60/292,694, filed May 22, 2001 entitled “Gene Marking Study of Periosteum-Derived Cells Using Adeno-Associated Virus Vector” from which application priority is claimed under 35 USC §119(e)(1).
Provisional Applications (1)
|
Number |
Date |
Country |
|
60292694 |
May 2001 |
US |