Claims
- 1. A globin-like polypeptide comprising two dialpha domains.
- 2. The globin-like polypeptide of claim 1, wherein said polypeptide contains a non-naturally occurring cysteine residue.
- 3. A hemoglobin-like molecule comprising at least two connected globin-like polypeptides of claim 1, wherein said connection consists of direct connection through a disulfide bond or indirect connection through a chemical crosslinker selected from the group consisting of homobifunctional linkers, heterobifunctional linkers, homopolyfunctional linkers and heteropolyfunctional linkers.
- 4. The globin-like polypeptide of claim 2, wherein said non-naturally occurring cysteine residue is asymmetric.
- 5. A multimeric hemoglobin-like protein comprising a core hemoglobin-like moiety to which each of at least two other hemoglobin-like moieties are directly attached to the core hemoglobin-like moiety.
- 6. The multimeric hemoglobin-like protein of claim 5, wherein the other hemoglobin-like moieties are attached to the core hemoglobin-like moiety by a chemical crosslinker.
- 7. The multimeric hemoglobin-like protein of claim 5, wherein the other hemoglobin-like moieties are attached to the core hemoglobin-like moiety by a N-.gamma.-maleimidobutyrloxysuccinimide ester.
- 8. The multimeric hemoglobin-like protein of claim 7, wherein the core hemoglobin-like moiety is rHb1.1 and the other hemoglobin-like moieties are K158C.
- 9. A method for separation of molecular weight fractions of polymerized hemoglobin-like molecules to obtain substantially monodisperse hemoglobin solutions comprising:
- (a) contacting a polydisperse mixture of polymerized hemoglobin-like molecules with an ion exchange matrix;
- (b) washing the ion exchange matrix with a first buffer;
- (c) eluting the ion exchange matrix with a second buffer which can be the same or different from said first buffer to obtain a substantially monodisperse hemoglobin-like solution.
- 10. The method of claim 9, wherein the ion exchange matrix is an anion exchange matrix.
Parent Case Info
This application is the U.S. national phase of International Application No. PCT/US96/10420, filed Jun. 6, 1996, which is a continuation-in-part of U.S. patent application Ser. No. 08/487,431, filed Jun. 7, 1995, now U.S. Pat. No. 5,844,090, which is a continuation-in-part of U.S. patent application Ser. No. 08/240,712, filed May 9, 1994, now U.S. Pat No. 5,599,907, which was the U.S. national phase of International application No. PCT/US92/09752, filed Nov. 6, 1992., which is a continuation in part of U.S. patent application Ser. No. 07/789,179, filed Nov. 8, 1991, now U.S. Pat. No. 5,545,727, which is a continuation in part of PCT/US90/02654, filed May 10, 1990, which is a continuation in part of U.S. patent application Ser. No. 07/379,116, filed Jul. 13, 1989, now abandoned, U.S. application Ser. No. 07/374,161, filed Jun. 30, 1989, now abandoned, and U.S. patent application Ser. No. 07/349,623, filed May 10, 1989, now abandoned.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
102e Date |
371c Date |
PCT/US96/10420 |
6/6/1996 |
|
|
8/24/1998 |
8/24/1998 |
Publishing Document |
Publishing Date |
Country |
Kind |
WO96/40920 |
12/19/1996 |
|
|
US Referenced Citations (11)
Foreign Referenced Citations (6)
Number |
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9013645 |
Nov 1990 |
WOX |
9116349 |
Oct 1991 |
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9211283 |
Jul 1992 |
WOX |
9222646 |
Dec 1992 |
WOX |
9309143 |
May 1993 |
WOX |
9308831 |
May 1993 |
WOX |
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Continuation in Parts (2)
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Number |
Date |
Country |
Parent |
487431 |
Jun 1995 |
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Parent |
240712 |
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