Modified transferrin fusion proteins

FIELD OF THE INVENTION

[0002] The present invention relates to therapeutic proteins or peptides with extended serum stability or serum half-life, particularly to therapeutic proteins or peptides fused to or inserted in a transferrin molecule modified to reduce or inhibit glycosylation, iron binding and/or transferrin receptor binding.

BACKGROUND OF THE INVENTION

[0003] Therapeutic proteins or peptides in their native state or when recombinantly produced are typically labile molecules exhibiting short periods of serum stability or short serum half-lives. In addition, these molecules are often extremely labile when formulated, particularly when formulated in aqueous solutions for diagnostic and therapeutic purposes.

[0004] Few practical solutions exist to extend or promote the stability in vivo or in vitro of proteinaceous therapeutic molecules. Polyethylene glycol (PEG) is a substance that can attach to a protein, resulting in longer-acting, sustained activity of the protein. If the activity of a protein is prolonged by the attachment to PEG, the frequency that the protein needs to be administered is decreased. PEG attachment, however, often decreases or destroys the protein's therapeutic activity.

[0005] Therapeutic proteins or peptides have also been stabilized by fusion to a heterologous protein capable of extending the serum half-life of the therapeutic protein. For instance, therapeutic proteins fused to albumin and antibody fragments may exhibit extended serum half-live when compared to the therapeutic protein in the unfused state. See U.S. Pat. Nos. 5,876,969 and 5,766,88.

[0006] Another serum protein, glycosylated human transferrin (Tf) has also been used to make fusions with therapeutic proteins to target delivery intracellularly or to carry heterologous agents across the blood-brain barrier. These fusion proteins comprising glycosylated human Tf have been used to target nerve growth factor (NGF) or ciliary neurotrophic factor (CNTF) across the blood-brain barrier by fusing full-length Tf to the either agent. See U.S. Pat. Nos. 5,672,683 and 5977,307. In these fusion proteins, the Tf portion of the molecule is glycosylated and binds to two atoms of iron, which is required for Tf binding to its receptor on a cell and, according to the inventors of these patents, to target delivery of the NGF or CNTF moiety across the blood-brain barrier. Transferrin fusion proteins have also been produced by inserting an HIV-1 protease sequence into surface exposed loops of glycosylated transferrin to investigate the ability to produce another form of Tf fusion for targeted delivery to the inside of a cell via the Tf receptor (Ali et al. (1999) J. Biol. Chem. 274(34):24066-24073).

[0007] Serum transferrin (Tf) is a monomeric glycoprotein with a molecular weight of 80,000 daltons that binds iron in the circulation and transports it to various tissues via the transferrin receptor (TfR) (Aisen et al. (1980) Ann. Rev. Biochem. 49: 357-393; MacGillivray et al. (1981) J. Biol. Chem. 258: 3543-3553, U.S. Pat. No. 5,026,651). Tf is one of the most common serum molecules, comprising up to about 5-10% of total serum proteins. Carbohydrate deficient transferrin occurs in elevated levels in the blood of alcoholics and exhibits a longer half life (approximately 14-17 days) than that of glycosylated transferrin (approximately 7-10 days). See van Eijk et al. (1983) Clin. Chim. Acta 132:167-171, Stibler (1991) Clin. Chem. 37:2029-2037 (1991), Arndt (2001) Clin. Chem. 47(1):13-27 and Stibler et al. in “Carbohydrate-deficient consumption”, Advances in the Biosciences, (Ed Nordmann et al.), Pergamon, 1988, Vol. 71, pages 353-357).

[0008] The structure of Tf has been well characterized and the mechanism of receptor binding, iron binding and release and carbonate ion binding have been eluciated (U.S. Pat. Nos. 5,026,651, 5,986,067 and MacGillivray et al. (1983) J. Biol. Chem. 258(6):3543-3546).

[0009] Transferrin and antibodies that bind the transferrin receptor have also been used to deliver or carry toxic agents to tumor cells as cancer therapy (Baselga and Mendelsohn, 1994), and transferrin has been used as a non-viral gene therapy vector to vehicle to deliver DNA to cells (Frank et al., 1994; Wagner et al., 1992). The ability to deliver proteins to the central nervous system (CNS) using the transferrin receptor as the entry point has been demonstrated with several proteins and peptides including CD4 (Walus et al., 1996), brain derived neurotrophic factor (Pardridge et al., 1994), glial derived neurotrophic factor (Albeck et al.), a vasointestinal peptide analogue (Bickel et al., 1993), a betaamyloid peptide (Saito et al., 1995), and an antisense oligonucleotide (Pardridge et al., 1995).

[0010] Transferrin fusion proteins have not, however, been modified or engineered to extend the serum half-life of a therapeutic protein or peptide or to increase bioavailability by reducing or inhibiting glycosylation of the Tf moiety or to reduce or prevent iron and/or Tf receptor binding.

SUMMARY OF THE INVENTION

[0011] As described in more detail below, the present invention includes modified Tf fusion proteins comprising at least one therapeutic protein, polypeptide or peptide entity, wherein the Tf portion is engineered to extend the serum half-life or bioavailability of the molecule. The invention also includes pharmaceutical formulations and compositions comprising the fusion proteins, methods of extending the serum stability, serum half-life and bioavailability of a therapeutic protein by fusion to modified transferrin, nucleic acid molecules encoding the modified Tf fusion proteins, and the like. Another aspect of the present invention relates to methods of treating a patient with a modified Tf fusion protein.

[0012] In a preferred embodiment, the modified Tf fusion proteins comprise a human transferrin Tf moiety that has been modified to reduce or prevent glycosylation and/or iron and receptor binding.

BRIEF DESCRIPTION OF THE DRAWINGS

[0013]
FIG. 1 shows an alignment of the N and C Domains of Human (Hu) transferrin (Tf) with similarities and identities highlighted.

[0014] FIGS. 2A-2B shows an alignment of transferrin sequences from different species. Light shading: Similarity; Dark shading: Identity

[0015]
FIG. 3 shows the location of a number of Tf surface exposed insertion sites for therapeutic proteins, polypeptides or peptides.

[0016] FIGS. 4A-4B shows the VH and VL regions for a number of preferred anti-TNFα antibodies used to produce modified Tf fusion proteins.

[0017]
FIG. 5 shows pREX0010

[0018]
FIG. 6 shows pREX0011

[0019]
FIG. 7 shows pREX0012

[0020]
FIG. 8 shows pREX0013

[0021]
FIG. 9 shows pREX0014

[0022]
FIG. 10 shows pREX0015

DETAILED DESCRIPTION

[0023] General Description

[0024] It has been discovered that a therapeutic protein (e.g., a polypeptide, antibody, or peptide, or fragments and variants thereof) can be stabilized to extend the serum half-life and/or retain the therapeutic protein's activity for extended periods of time in vivo by genetically fusing or chemically conjugating the therapeutic protein, polypeptide or peptide to all or a portion of modified transferrin sufficient to extend its half life in serum. The modified transferrin fusion proteins include a transferrin protein or domain covalently linked to a therapeutic protein or peptide, wherein the transferrin portion is modified to contain one or more amino acid substitutions, insertions or deletions compared to a wild-type transferrin sequence. In one embodiment, Tf fusion proteins are engineered to reduce or prevent glycosylation within the Tf or a Tf domain. In other embodiments, the Tf protein or Tf domain(s) is modified to exhibit reduced or no binding to iron or carbonate ion, or to have a reduced affinity or not bind to a Tf receptor (TfR).

[0025] The present invention therefore includes transferrin fusion proteins, therapeutic compositions comprising the fusion proteins, and methods of treating, preventing, or ameliorating diseases or disorders by administering the fusion proteins. A transferrin fusion protein of the invention includes at least a fragment or variant of a therapeutic protein and at least a fragment or variant of modified transferrin, which are associated with one another, preferably by genetic fusion (i.e., the transferrin fusion protein is generated by translation of a nucleic acid in which a polynucleotide encoding all or a portion of a therapeutic protein is joined in-frame with a polynucleotide encoding all or a portion of modified transferrin) or chemical conjugation to one another. The therapeutic protein and transferrin protein, once part of the transferrin fusion protein, may be referred to as a “portion”, “region” or “moiety” of the transferrin fusion protein (e.g., a “therapeutic protein portion” or a “transferrin protein portion”).

[0026] In one embodiment, the invention provides a transferrin fusion protein comprising, or alternatively consisting of, a therapeutic protein and a modified serum transferrin protein. In other embodiments, the invention provides a transferrin fusion protein comprising, or alternatively consisting of, a biologically active and/or therapeutically active fragment of a therapeutic protein and a modified transferrin protein. In other embodiments, the invention provides a transferrin fusion protein comprising, or alternatively consisting of, a biologically active and/or therapeutically active variant of a therapeutic protein and modified transferrin protein. In further embodiments, the invention provides a transferrin fusion protein comprising a therapeutic protein, and a biologically active and/or therapeutically active fragment of modified transferrin. In another embodiment, the therapeutic protein portion of the transferrin fusion protein is the active form of the therapeutic protein.

[0027] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, the preferred methods and materials are described.

[0028] Definitions

[0029] As used herein, the term “biological activity” refers to a function or set of activities performed by a therapeutic molecule, protein or peptide in a biological context (i.e., in an organism or an in vitro facsimile thereof). Biological activities may include but are not limited to the functions of the therapeutic molecule portion of the claimed fusion proteins, such as, but not limited to, the induction of extracellular matrix secretion from responsive cell lines, the induction of hormone secretion, the induction of chemotaxis, the induction of mitogenesis, the induction of differentiation, or the inhibition of cell division of responsive cells. A fusion protein or peptide of the invention is considered to be biologically active if it exhibits one or more biological activities of its therapeutic protein's native counterpart.

[0030] As used herein, an “amino acid corresponding to” or an “equivalent amino acid” in a transferrin sequence is identified by alignment to maximize the identity or similarity between a first transferrin sequence and at least a second transferrin sequence. The number used to identify an equivalent amino acid in a second transferrin sequence is based on the number used to identify the corresponding amino acid in the first transferrin sequence. In certain cases, these phrases may be used to describe the amino acid residues in human transferrin compared to certain residues in rabbit serum transferrin.

[0031] As used herein, the terms “fragment of a Tf protein” or “Tf protein,” or “portion of a Tf protein” refer to an amino acid sequence comprising at least about 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, 99% or 100% of a naturally occurring Tf protein or mutant thereof.

[0032] As used herein, the term “gene” refers to any segment of DNA associated with a biological function. Thus, genes include, but are not limited to, coding sequences and/or the regulatory sequences required for their expression. Genes can also include nonexpressed DNA segments that, for example, form recognition sequences for other proteins. Genes can be obtained from a variety of sources, including cloning from a source of interest or synthesizing from known or predicted sequence information, and may include sequences designed to have desired parameters.

[0033] As used herein, a “heterologous polynucleotide” or a “heterologous nucleic acid” or a “heterologous gene” or a “heterologous sequence” or an “exogenous DNA segment” refers to a polynucleotide, nucleic acid or DNA segment that originates from a source foreign to the particular host cell, or, if from the same source, is modified from its original form. A heterologous gene in a host cell includes a gene that is endogenous to the particular host cell, but has been modified. Thus, the terms refer to a DNA segment which is foreign or heterologous to the cell, or homologous to the cell but in a position within the host cell nucleic acid in which the element is not ordinarily found. As an example, a signal sequence native to a yeast cell but attached to a human Tf sequence is heterologous.

[0034] As used herein, an “isolated” nucleic acid sequence refers to a nucleic acid sequence which is essentially free of other nucleic acid sequences, e.g., at least about 20% pure, preferably at least about 40% pure, more preferably about 60% pure, even more preferably about 80% pure, most preferably about 90% pure, and even most preferably about 95% pure, as determined by agarose gel electrophoresis. For example, an isolated nucleic acid sequence can be obtained by standard cloning procedures used in genetic engineering to relocate the nucleic acid sequence from its natural location to a different site where it will be reproduced. The cloning procedures may involve excision and isolation of a desired nucleic acid fragment comprising the nucleic acid sequence encoding the polypeptide, insertion of the fragment into a vector molecule, and incorporation of the recombinant vector into a host cell where multiple copies or clones of the nucleic acid sequence will be replicated. The nucleic acid sequence may be of genomic, cDNA, RNA, semisynthetic, synthetic origin, or any combinations thereof.

[0035] As used herein, two or more DNA coding sequences are said to be “joined” or “fused” when, as a result of in-frame fusions between the DNA coding sequences, the DNA coding sequences are translated into a polypeptide fusion. The term “fusion” in reference to Tf fusions includes, but is not limited to, attachment of at least one therapeutic protein, polypeptide or peptide to the N-terminal end of Tf, attachment to the C-terminal end of Tf, and/or insertion between any two amino acids within Tf.

[0036] “Modified transferrin” as used herein refers to a transferrin molecule that exhibits at least one modification of its amino acid sequence, compared to wildtype transferrin.

[0037] “Modified transferrin fusion protein” as used herein refers to a protein formed by the fusion of at least one molecule of modified transferrin (or a fragment or variant thereof) to at least one molecule of a therapeutic protein (or fragment or variant thereof).

[0038] As used herein, the terms “nucleic acid” or “polynucleotide” refer to deoxyribonucleotides or ribonucleotides and polymers thereof in either single- or double-stranded form. Unless specifically limited, the terms encompass nucleic acids containing analogues of natural nucleotides that have similar binding properties as the reference nucleic acid and are metabolized in a manner similar to naturally occurring nucleotides. Unless otherwise indicated, a particular nucleic acid sequence also implicitly encompasses conservatively modified variants thereof (e.g. degenerate codon substitutions) and complementary sequences as well as the sequence explicitly indicated. Specifically, degenerate codon substitutions may be achieved by generating sequences in which the third position of one or more selected (or all) codons is substituted with mixed-base and/or deoxyinosine residues (Batzer et al. (1991) Nucleic Acid Res. 19:5081; Ohtsuka et al. (1985) J. Biol. Chem. 260:2605-2608; Cassol et al. (1992); Rossolini et al. (1994) Mol. Cell. Probes 8:91-98). The term nucleic acid is used interchangeably with gene, cDNA, and mRNA encoded by a gene.

[0039] As used herein, a DNA segment is referred to as “operably linked” when it is placed into a functional relationship with another DNA segment. For example, DNA for a signal sequence is operably linked to DNA encoding a fusion protein of the invention if it is expressed as a preprotein that participates in the secretion of the fusion protein; a promoter or enhancer is operably linked to a coding sequence if it stimulates the transcription of the sequence. Generally, DNA sequences that are operably linked are contiguous, and in the case of a signal sequence or fusion protein both contiguous and in reading phase. However, enhancers need not be contiguous with the coding sequences whose transcription they control. Linking, in this context, is accomplished by ligation at convenient restriction sites or at adapters or linkers inserted in lieu thereof.

[0040] As used herein, the term “promoter” refers to a region of DNA involved in binding RNA polymerase to initiate transcription.

[0041] As used herein, the term “recombinant” refers to a cell, tissue or organism that has undergone transformation with recombinant DNA.

[0042] As used herein, a targeting entity, protein, polypeptide or peptide refers to such molecules that binds specifically to a particular cell type [normal (e.g., lymphocytes) or abnormal e.g., (cancer cell)] and therefore may be used to target a Tf fusion protein or compound (drug, or cytotoxic agent) to that cell type specifically.

[0043] As used herein, “therapeutic protein” refers to proteins, polypeptides, antibodies, peptides or fragments or variants thereof, having one or more therapeutic and/or biological activities. Therapeutic proteins encompassed by the invention include but are not limited to proteins, polypeptides, peptides, antibodies, and biologics. The terms peptides, proteins, and polypeptides are used interchangeably herein. Additionally, the term “therapeutic protein” may refer to the endogenous or naturally occurring correlate of a therapeutic protein. By a polypeptide displaying a “therapeutic activity” or a protein that is “therapeutically active” is meant a polypeptide that possesses one or more known biological and/or therapeutic activities associated with a therapeutic protein such as one or more of the therapeutic proteins described herein or otherwise known in the art. As a non-limiting example, a “therapeutic protein” is a protein that is useful to treat, prevent or ameliorate a disease, condition or disorder. Such a disease, condition or disorder may be in humans or in a non-human animal, e.g., veterinary use.

[0044] As used herein, the term “transformation” refers to the transfer of nucleic acid (i.e., a nucleotide polymer) into a cell. As used herein, the term “genetic transformation” refers to the transfer and incorporation of DNA, especially recombinant DNA, into a cell.

[0045] As used herein, the term “transformant” refers to a cell, tissue or organism that has undergone transformation.

[0046] As used herein, the term “transgene” refers to a nucleic acid that is inserted into an organism, host cell or vector in a manner that ensures its function.

[0047] As used herein, the term “transgenic” refers to cells, cell cultures, organisms, bacteria, fungi, animals, plants, and progeny of any of the preceding, which have received a foreign or modified gene and in particular a gene encoding a modified Tf fusion protein by one of the various methods of transformation, wherein the foreign or modified gene is from the same or different species than the species of the organism receiving the foreign or modified gene.

[0048] “Variants or variant” refers to a polynucleotide or nucleic acid differing from a reference nucleic acid or polypeptide, but retaining essential properties thereof. Generally, variants are overall closely similar, and, in many regions, identical to the reference nucleic acid or polypeptide. As used herein, “variant”, refers to a therapeutic protein portion of a transferrin fusion protein of the invention, differing in sequence from a native therapeutic protein but retaining at least one functional and/or therapeutic property thereof as described elsewhere herein or otherwise known in the art.

[0049] As used herein, the term “vector” refers broadly to any plasmid, phagemid or virus encoding an exogenous nucleic acid. The term is also be construed to include non-plasmid, non-phagemid and non-viral compounds which facilitate the transfer of nucleic acid into virions or cells, such as, for example, polylysine compounds and the like. The vector may be a viral vector that is suitable as a delivery vehicle for delivery of the nucleic acid, or mutant thereof, to a cell, or the vector may be a non-viral vector which is suitable for the same purpose. Examples of viral and non-viral vectors for delivery of DNA to cells and tissues are well known in the art and are described, for example, in Ma et al. (1997, Proc. Natl. Acad. Sci. U.S.A. 94:12744-12746). Examples of viral vectors include, but are not limited to, a recombinant vaccinia virus, a recombinant adenovirus, a recombinant retrovirus, a recombinant adeno-associated virus, a recombinant avian pox virus, and the like (Cranage et al., 1986, EMBO J. 5:3057-3063; International Patent Application No. WO94/17810, published Aug. 18, 1994; International Patent Application No. WO94/23744, published Oct. 27, 1994). Examples of non-viral vectors include, but are not limited to, liposomes, polyamine derivatives of DNA, and the like.

[0050] As used herein, the term “wild type” refers to a polynucleotide or polypeptide sequence that is naturally occurring.

[0051] Transferrin and Transferrin Modifications

[0052] Any transferrin may be used to make modified Tf fusion proteins of the invention. Wild-type human Tf (Tf) is a 679 amino acid protein, of approximately 75 kDa (not accounting for glycosylation), with two main domains, N (about 330 amino acids) and C (about 340 amino acids), which appear to originate from a gene duplication. See GenBank accession numbers NM001063, XM002793, M12530, XM039845, XM 039847 and S95936 (www.ncbi.nlm.nih.gov/), all of which are herein incorporated by reference in their entirety, as well as SEQ ID NOS 1, 2 and 3. The two domains have diverged over time but retain a large degree of identity/similarity (FIG. 1).

[0053] Each of the N and C domains is further divided into two subdomains, N1 and N2, C1 and C2. The function of Tf is to transport iron to the cells of the body. This process is mediated by the Tf receptor (TfR), which is expressed on all cells, particularly actively growing cells. TfR recognizes the iron bound form of Tf (two of which are bound per receptor), endocytosis then occurs whereby the TfR/Tf complex is transported to the endosome, at which point the localized drop in pH results in release of bound iron and the recycling of the TfR/Tf complex to the cell surface and release of Tf (known as apoTf in its un-iron bound form). Receptor binding is through the C domain of Tf. The two glycosylation sites in the C domain do not appear to be involved in receptor binding as unglycosylated iron bound Tf does bind the receptor.

[0054] Each Tf molecule can carry two iron atoms. These are complexed in the space between the N1 and N2, C1 and C2 sub domains resulting in a conformational change in the molecule. Tf crosses the blood brain barrier (BBB) via the Tf receptor.

[0055] In human transferrin, the iron binding sites comprise at least of amino acids Asp 63 (Asp 82 of SEQ ID NO: 2 which comprises the native Tf signal sequence); Asp 392 (Asp 411 of SEQ ID NO: 2); Tyr 95 (Tyr 114 of SEQ ID NO: 2); Tyr 426 (Tyr 445 of SEQ ID NO: 2); Tyr 188 (Tyr 207 of SEQ ID NO: 2); Tyr 514 or 517 (Tyr 533 or Tyr 536 SEQ ID NO:2); His 249 (His 268 of SEQ ID NO: 2); His 585 (His 604 of SEQ ID NO: 2), the hinge regions comprises of at least N domain amino acid residues 94-96, 245-247 and/or 316-318 as well as C domain amino acid residues 425-427, 581-582 and/or 652-658., the carbonate binding sites comprise at least of amino acids Thr 120 (Thr 139 of SEQ ID NO: 2); Thr 452 (Thr 471 of SEQ ID NO: 2); Arg 124 (Arg 143 of SEQ ID NO: 2); Arg 456 (Arg 475 of SEQ ID NO: 2); Ala 126 (Ala 145 of SEQ ID NO: 2); Ala 458 (Ala 477 of SEQ ID NO: 2); Gly 127 (Gly 146 of SEQ ID NO: 2); Gly 459 (Gly 478 of SEQ ID NO: 2).

[0056] In one embodiment of the invention, the modified transferrin fusion protein includes a modified human transferrin, although any animal Tf molecule may be used to produce the fusion proteins of the invention, including human Tf variants, cow, pig, sheep, dog, rabbit, rat, mouse, hamster, echnida, platypus, chicken, frog, hornworm, monkey, as well as other bovine, canine and avian species (see FIG. 2 for a representative set of Tf sequences). All of these Tf sequences are readily available in GenBank and other public databases. The human Tf nucleotide sequence is available (see SEQ ID NOS 1, 2 and 3 and the accession numbers described above and available at www.ncbi.nlm.nih.gov/) and can be used to make genetic fusions between Tf or a domain of Tf and the therapeutic molecule of choice. Fusions may also be made from related molecules such as lacto transferrin (lactoferrin) GenBank Acc: NM—002343).

[0057] Lactoferrin (Lf), a natural defense iron-binding protein, has been found to possess antibacterial, antimycotic, antiviral, antineoplastic and anti-inflammatory activity. The protein is present in exocrine secretions that are commonly exposed to normal flora: milk, tears, nasal exudate, saliva, bronchial mucus, gastrointestinal fluids, cervico-vaginal mucus and seminal fluid. Additionally, Lf is a major constituent of the secondary specific granules of circulating polymorphonuclear neutrophils (PMNs). The apoprotein is released on degranulation of the PMNs in septic areas. A principal function of Lf is that of scavenging free iron in fluids and inflamed areas so as to suppress free radical-mediated damage and decrease the availability of the metal to invading microbial and neoplastic cells. In a study that examined the turnover rate of 125I Lf in adults, it was shown that LF is rapidly taken up by the liver and spleen, and the radioactivity persisted for several weeks in the liver and spleen (Bennett et al. (1979), Clin. Sci. (Lond.) 57: 453-460).

[0058] In another embodiment, the transferrin portion of the transferrin fusion protein of the invention includes a transferrin splice variant. In one example, a transferrin splice variant can be a splice variant of human transferrin. In one specific embodiment, the human transferrin splice variant can be that of Genbank Accession AAA61140. In another embodiment, the transferrin portion of the transferrin fusion protein of the invention includes a lactoferrin splice variant. In one example, a human serum lactoferrin splice variant can be a novel splice variant of a neutrophil lactoferrin. In one specific embodiment, the neutrophil lactoferrin splice variant can be that of Genbank Accession AAA59479. In another specific embodiment, the neutrophil lactoferrin splice variant can comprise the following amino acid sequence EDCIALKGEADA (SEQ ID NO: 8), which includes the novel region of splice-variance.

[0059] Modified Tf fusions may be made with any Tf protein, fragment, domain, or engineered domain. For instance, fusion proteins may be produced using the full-length Tf sequence, with or without the native Tf signal sequence. Tf fusion proteins may also be made using a single Tf domain, such as an individual N or C domain. In some embodiments, the use of a single N domain is advantageous as the Tf glycosylation sites reside in the C domain and the N domain, on its own, does not bind iron or the Tf receptor. In other embodiments, fusions of a therapeutic protein to a single C domain may be produced, wherein the C domain is altered to reduce, inhibit or prevent glycosylation, iron binding and/or Tf receptor binding.

[0060] In some embodiments, the Tf or Tf portion will be of sufficient length to increase the serum, in vitro solution stability or bioavailability of the therapeutic protein compared to the serum stability (half-life), in vitro stability or bioavailability of the therapeutic protein in an unfused state. Such an increase in stability, serum half-life or bioavailability may be about a 30%, 50%, 70%, 80%, 90% or more increase over the unfused therapeutic protein. In some cases, the modified transferrin fusion proteins exhibit a serum half-life of about 10-20 or more days, about 12-18 days or about 14-17 days.

[0061] When the C domain of Tf is part of the fusion protein, the two N-linked glycosylation sites, amino acid residues corresponding to N413 and N611 of SEQ ID NO:3 may be mutated for expression in a yeast system to prevent glycosylation or hypermannosylationn and extend the serum half-life of the fusion protein and/or therapeutic protein (to produce asialo-, or in some instances, monosialo-Tf or disialo-Tf). In addition to Tf amino acids corresponding to N413 and N611, mutations may be to the adjacent residues within the N-X-S/T glycosylation site to prevent or substantially reduce glycosylation. See U.S. Pat. No. 5,986,067 of Funk et al. It has also been reported that the N domain of Tf expressed in Pichia pastoris becomes O-linked glycosylated with a single hexose at S32 which also may be mutated or modified to prevent such glycosylation.

[0062] Accordingly, in one embodiment of the invention, the transferrin fusion protein includes a modified transferrin molecule wherein the transferrin exhibits reduced glycosylation, including but not limited to asialo- monosialo- and disialo-forms of Tf. In another embodiment, the transferrin portion of the transferrin fusion protein includes a recombinant transferrin mutant that is mutated to prevent glycosylation. In another embodiment, the transferrin portion of the transferrin fusion protein includes a recombinant transferrin mutant that is fully glycosylated. In a further embodiment, the transferrin portion of the transferrin fusion protein includes a recombinant human serum transferrin mutant that is mutated to prevent glycosylation, wherein at least one of Asn413 and Asn611 of SEQ ID NO:3 are mutated to an amino acid which does not allow glycosylation. In another embodiment, the transferrin portion of the transferrin fusion protein includes a recombinant human serum transferrin mutant that is mutated to prevent or substantially reduce glycosylation, wherein mutations may be to the adjacent residues within the N-X-S/T glycosylation site As discussed below in more detail, modified Tf fusion proteins of the invention may also be engineered to not bind iron and/or not bind the Tf receptor. In other embodiments of the invention, the iron binding is retained and the iron binding ability of Tf may be used in two ways, one to deliver a therapeutic protein or peptide(s) to the inside of a cell and/or across the BBB. These embodiments that bind iron and/or the Tf receptor will often be engineered to reduce or prevent glycosylation to extend the serum half-life of the therapeutic protein. The N domain alone will not bind to TfR when loaded with iron, and the iron bound C domain will bind TfR but not with the same affinity as the whole molecule.

[0063] In another embodiment, the transferrin portion of the transferrin fusion protein includes a recombinant transferrin mutant having a mutation wherein the mutant does not retain the ability to bind metal. In an alternate embodiment, the transferrin portion of the transferrin fusion protein includes a recombinant transferrin mutant having a mutation wherein the mutant has a weaker binding avidity for metal than wild-type serum transferrin. In an alternate embodiment, the transferrin portion of the transferrin fusion protein includes a recombinant transferrin mutant having a mutation wherein the mutant has a stronger binding avidity for metal than wild-type serum transferrin.

[0064] In another embodiment, the transferrin portion of the transferrin fusion protein includes a recombinant transferrin mutant having a mutation wherein the mutant does not retain the ability to bind to the transferrin receptor. In an alternate embodiment, the transferrin portion of the transferrin fusion protein includes a recombinant transferrin mutant having a mutation wherein the mutant has a weaker binding avidity for the transferrin receptor than wild-type serum transferrin. In an alternate embodiment, the transferrin portion of the transferrin fusion protein includes a recombinant transferrin mutant having a mutation wherein the mutant has a stronger binding avidity for the transferrin receptor than wild-type serum transferrin.

[0065] In another embodiment, the transferrin portion of the transferrin fusion protein includes a recombinant transferrin mutant having a mutation wherein the mutant does not retain the ability to bind to carbonate. In an alternate embodiment, the transferrin portion of the transferrin fusion protein includes a recombinant transferrin mutant having a mutation wherein the mutant has a weaker binding avidity for carbonate than wild-type serum transferrin. In an alternate embodiment, the transferrin portion of the transferrin fusion protein includes a recombinant transferrin mutant having a mutation wherein the mutant has a stronger binding avidity for carbonate than wild-type serum transferrin.

[0066] In another embodiment, the transferrin portion of the transferrin fusion protein includes a recombinant human serum transferrin mutant having a mutation in at least one amino acid residue selected from the group consisting of Asp63, Gly65, Tyr95, Tyr188, His249, Asp392, Tyr426, Tyr514, Tyr517 and His585 of SEQ ID NO:3, wherein the mutant retains the ability to bind metal. In an alternate embodiment, a recombinant human serum transferrin mutant having a mutation in at least one amino acid residue selected from the group consisting of Asp63, Gly65, Tyr95, Tyr188, His249, Asp392, Tyr426, Tyr514, Tyr517 and His585 of SEQ ID NO:3, wherein the mutant has a reduced ability to bind metal. In another embodiment, a recombinant human serum transferrin mutant having a mutation in at least one amino acid residue selected from the group consisting of Asp63, Gly65, Tyr95, Tyr188, His249, Asp392, Tyr426, Tyr517 and His585 of SEQ ID NO:3, wherein the mutant does not retain the ability to bind metal.

[0067] In another embodiment, the transferrin portion of the transferrin fusion protein includes a recombinant human serum transferrin mutant having a mutation at Lys206 or His207 of SEQ ID NO:3, wherein the mutant has a stronger binding avidity for metal than wild-type human serum transferrin (see U.S. Pat. No. 5,986,067, which is herein incorporated by reference in its entirety). In an alternate embodiment, the transferrin portion of the transferrin fusion protein includes a recombinant human serum transferrin mutant having a mutation at Lys206 or His207 of SEQ ID NO:3, wherein the mutant has a weaker binding avidity for metal than wild-type human serum transferrin. In a further embodiment, the transferrin portion of the transferrin fusion protein includes a recombinant human serum transferrin mutant having a mutation at Lys206 or His207 of SEQ ID NO:3, wherein the mutant does not bind metal.

[0068] Any available technique may be used to make the fusion proteins of the invention, including but not limited to molecular techniques commonly available, for instance, those disclosed in Sambrook et al. Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory Press, 1989. When carrying out nucleotide substitutions using techniques for accomplishing site-specific mutagenesis that are well known in the art, the encoded amino acid changes are preferably of a minor nature, that is, conservative amino acid substitutions, although other, non-conservative, substitutions are contemplated as well, particularly when producing a modified transferrin portion of a Tf fusion protein, e.g., a modified Tf fusion protein exhibiting reduced glycosylation, reduced iron binding and the like. Specifically contemplated are amino acid substitutions, small deletions or insertions, typically of one to about 30 amino acids; insertions between transferrin domains; small amino- or carboxyl-terminal extensions, such as an amino-terminal methionine residue, or small linker peptides of less than 50, 40, 30, 20 or 10 residues between transferrin domains or linking a transferrin protein and a therapeutic protein or peptide; or a small extension that facilitates purification, such as a poly-histidine tract, an antigenic epitope or a binding domain.

[0069] Examples of conservative amino acid substitutions are substitutions made within the same group such as within the group of basic amino acids (such as arginine, lysine, histidine), acidic amino acids (such as glutamic acid and aspartic acid), polar amino acids (such as glutamine and asparagine), hydrophobic amino acids (such as leucine, isoleucine, valine), aromatic amino acids (such as phenylalanine, tryptophan, tyrosine) and small amino acids (such as glycine, alanine, serine, threonine, methionine).

[0070] Non-conservative substitutions encompass substitutions of amino acids in one group by amino acids in another group. For example, a non-conservative substitution would include the substitution of a polar amino acid for a hydrophobic amino acid. For a general description of nucleotide substitution, see e.g. Ford et al. (1991), Prot. Exp. Pur. 2: 95-107. Non-conservative substitutions, deletions and insertions are particularly useful to produce TF fusion proteins of the invention that exhibit no or reduced binding of iron, no or reduced binding of the fusion protein to the Tf receptor and/or no or reduced glycosylation.

[0071] In the polypeptide and proteins of the invention, the following system is followed for designating amino acids in accordance with the following conventional list:

1TABLE OF AMINO ACIDSONE-LETTERTHREE-LETTERAMINO ACIDSYMBOLSYMBOLAlanineAAlaArginineRArgAsparagineNAsnAspartic AcidDAspCysteineCCysGlutamineQGlnGlutamic AcidEGluGlycineGGlyHistidineHHisIsoleucineIIleLeucineLLeuLysineKLysMethionineMMetPhenylalanineFPheProlinePProSerineSSerThreonineTThrTryptophanWTrpTyrosineYTyrValineVVal

[0072] Iron binding and/or receptor binding may be reduced or disrupted by mutation, including deletion, substitution or insertion into, amino acid residues corresponding to one or more of Tf N domain residues Asp63, Tyr95, Tyr188, His249 and/or C domain residues Asp 392, Tyr 426, Tyr 514 and/or His 585. Iron binding may also be affected by mutation to amino acids Lys206, Hys207 or Arg632. Carbonate binding may be reduced or disrupted by mutation, including deletion, substitution or insertion into, amino acid residues corresponding to one or more of Tf N domain residues Thr120, Arg124, Ala126, Gly 127 and/or C domain residues Thr 452, Arg 456, Ala 458 and/or Gly 459. A reduction or disruption of carbonate binding may adversely affect iron and/or receptor binding.

[0073] Binding to the Tf receptor may be reduced or disrupted by mutation, including deletion, substitution or insertion into, amino acid residues corresponding to one or more of Tf N domain residues described above for iron binding.

[0074] As discussed above, glycosylation may be reduced or prevented by mutation, including deletion, substitution or insertion into, amino acid residues corresponding to one or more of Tf C domain residues around the N-X-S/T sites corresponding to C domain residues N413 and/or N611 (See U.S. Pat. No. 5,986,067). For instance, the N413 and/or N611 may be mutated to Glu residues.

[0075] In instances where the Tf fusion proteins of the invention are not modified to prevent glycosylation, iron binding, carbonate binding and/or receptor binding, glycosylation, iron and/or carbonate ions may be stripped from or cleaved off of the fusion protein. For instance, available de-glycosylases may be used to cleave glycosylation residues from the fusion protein, in particular the sugar residues attached to the Tf portion, yeast deficient in glycosylation enzymes may be used to prevent glycosylation and/or recombinant cells may be grown in the presence of an agent that prevents glycosylation, e.g., tunicamycin.

[0076] Additional mutations may be made with Tf to alter the three dimensional structure of TF, such as modifications to the hinge region to prevent Tf folding needed for iron biding and Tf receptor recognition. For instance, mutations may be made in or around N domain amino acid residues 94-96, 245-247 and/or 316-318 as well as C domain amino acid residues 425-427, 581-582 and/or 652-658. In addition, mutations may be made in to or around the flanking regions of these sites to alter Tf structure and function.

[0077] In one aspect of the invention, the transferrin fusion protein can function as a carrier protein to extend the half life or bioavailability of the therapeutic protein as well as in some instances, delivering the therapeutic protein inside a cell and/or across the blood brain barrier. In an alternate embodiment, the transferrin fusion protein includes a modified transferrin molecule wherein the transferrin does not retain the ability to cross the blood brain barrier.

[0078] In another embodiment, the transferrin fusion protein includes a modified transferrin molecule wherein the transferrin molecule retains the ability to bind to the transferrin receptor and transport the therapeutic peptide inside cells. In an alternate embodiment, the transferrin fusion protein includes a modified transferrin molecule wherein the transferrin molecule does not retain the ability to bind to the transferrin receptor and transport the therapeutic peptide inside cells.

[0079] In further embodiments, the transferrin fusion protein includes a modified transferrin molecule wherein the transferrin molecule retains the ability to bind to the transferrin receptor and transport the therapeutic peptide inside cells, but does not retain the ability to cross the blood brain barrier. In an alternate embodiment, the transferrin fusion protein includes a modified transferrin molecule wherein the transferrin molecule retains the ability to cross the blood brain barrier, but does not retain the ability to bind to the transferrin receptor and transport the therapeutic peptide inside cells.

[0080] Modified Transferrin Fusion Proteins

[0081] The fusion of proteins of the invention may contain one or more copies of the therapeutic protein attached to the N-terminus and/or the C-terminus of the Tf protein. In some embodiments, the therapeutic protein is attached to both the N- and C-terminus of the Tf protein and the fusion protein may contain one or more equivalents of the therapeutic protein on either or both ends of Tf. In other embodiments, the therapeutic protein or polypeptide is inserted into known domains of the Tf protein, for instance, into one or more of the loops of Tf (see Ali et al. (1999) J Biolog. Chem. 274(34):24066-24073). In other embodiments, the therapeutic protein or therapeutic peptide is inserted between the N and C domains of Tf.

[0082] Generally, the transferrin fusion protein of the inventions of the invention may have one modified transferrin-derived region and one therapeutic protein-derived region. Multiple regions of each protein, however, may be used to make a transferrin fusion protein of the invention. Similarly, more than one therapeutic protein may be used to make a transferrin fusion protein of the invention of the invention, thereby producing a multi-functional modified Tf fusion protein.

[0083] In one embodiment, the transferrin fusion protein of the invention contains a therapeutic protein or portion thereof fused to a transferrin molecule or portion thereof. In another embodiment, the transferrin fusion protein of the inventions contains a therapeutic protein fused to the N terminus of a transferrin molecule. In an alternate embodiment, the transferrin fusion protein of the invention contains a therapeutic protein fused to the C terminus of a transferrin molecule. In a further embodiment, the transferrin fusion protein of the invention contains a transferrin molecule fused to the N terminus of a therapeutic protein. In an alternate embodiment, the transferrin fusion protein of the invention contains a transferrin molecule fused to the C terminus of a therapeutic protein.

[0084] In further embodiments, the modified transferrin molecule contains the N terminus of a transferrin molecule fused to what would be the N terminus of a therapeutic peptide. In an alternate embodiment, the modified transferrin molecule contains the N terminus of a transferrin molecule fused to the C terminus of a therapeutic peptide. In a further alternate embodiment, the modified transferrin molecule contains the C terminus of a transferrin molecule fused to what would be the C terminus of a therapeutic peptide. In an alternate embodiment, the modified transferrin molecule contains the C terminus of a transferrin molecule fused to the N terminus of a therapeutic peptide.

[0085] In other embodiments, the transferrin fusion protein of the inventions contains a therapeutic protein fused to both the N-terminus and the C-terminus of modified transferrin. In another embodiment, the therapeutic proteins fused at the N- and C-termini are the same therapeutic proteins. In an alternate embodiment, the therapeutic proteins fused at the N- and C-termini are different therapeutic proteins. In another alternate embodiment, the therapeutic proteins fused to the N- and C-termini are different therapeutic proteins which may be used to treat or prevent the same disease, disorder, or condition. In another embodiment, the therapeutic proteins fused at the N- and C-termini are different therapeutic proteins which may be used to treat or prevent diseases or disorders which are known in the art to commonly occur in patients simultaneously.

[0086] In addition to modified transferrin fusion protein of the inventions in which the modified transferrin portion is fused to the N terminal and/or C-terminal of the therapeutic protein portion, transferrin fusion protein of the inventions of the invention may also be produced by inserting the therapeutic protein or peptide of interest (e.g., a therapeutic protein or peptide as disclosed herein, or, for instance, a single chain antibody that binds a therapeutic protein or a fragment or variant thereof) into an internal region of the modified transferrin. Internal regions of modified transferrin include, but are not limited to, the iron binding sites, the hinge regions, the bicarbonate binding sites, or the receptor binding domain.

[0087] Within the protein sequence of the modified transferrin molecule a number of loops or turns exist, which are stabilized by disulfide bonds. These loops are useful for the insertion, or internal fusion, of therapeutically active peptides, particularly those requiring a secondary structure to be functional, or therapeutic proteins, to essentially generate a modified transferrin molecule with specific biological activity.

[0088] When therapeutic proteins or peptides are inserted into or replace at least one loop of a Tf molecule, insertions may be made within any of the surface exposed loop regions, in addition to other areas of Tf. For instance, insertions may be made within the loops comprising TF amino acids 32-33, 74-75, 256-257, 279-280 and 288-289 (Ali et al., supra) (See FIG. 3). As previously described, insertions may also be made within other regions of Tf such as the sites for iron and bicarbonate binding, hinge regions, and the receptor binding domain as described in more detail below. The loops in the Tf protein sequence that are amenable to modification/replacement for the insertion of proteins or peptides may also be used for the development of a screenable library of random peptide inserts. Any procedures may be used to produce nucleic acid inserts for the generation of peptide libraries, including available phage and bacterial display systems, prior to cloning into a Tf domain and/or fusion to the ends of Tf.

[0089] The N-terminus of Tf is free and points away from the body of the molecule. Fusions of proteins or peptides on the N-terminus may therefore be a preferred embodiment. Such fusions may include a linker region, such as but not limited to a poly-glycine stretch, to separate the therapeutic protein or peptide from Tf. Attention to the junction between the leader sequence, the choice of leader sequence, and the structure of the mRNA by codon manipulation/optimization (no major stem loops to inhibit ribosome progress) will increase secretion and can be readily accomplished using standard recombinant protein techniques.

[0090] The C-terminus of Tf appears to be more buried and secured by a disulfide bond 6 amino acids from the C-terminus. In human Tf, the C-terminal amino acid is a proline which, depending on the way that it is orientated, will either point a fusion away or into the body of the molecule. A linker or spacer moiety at the C-terminus may be used in some embodiments of the invention.

[0091] In yet other embodiments, small molecule therapeutics may be complexed with iron and loaded on a modified Tf protein fusion for delivery to the inside of cells and across the BBB. The addition of a targeting peptide or, for example, a SCA will target the payload to a particular cell type, e.g., a cancer cell.

[0092] Nucleic Acids

[0093] Nucleic acid molecules are also provided by the present invention. These encode a modified Tf fusion protein comprising a transferrin protein or a portion of a transferrin protein covalently linked or joined to a therapeutic protein. As discussed in more detail below, any therapeutic protein may be used. The fusion protein may further comprise a linker region, for instance a linker less than about 50, 40, 30, 20, or 10 amino acid residues. The linker can be covalently linked to and between the transferrin protein or portion thereof and the therapeutic protein. Nucleic acid molecules of the invention may be purified or not.

[0094] Host cells and vectors for replicating the nucleic acid molecules and for expressing the encoded fusion proteins are also provided. Any vectors or host cells may be used, whether prokaryotic or eukaryotic, but eukaryotic expression systems, in particular yeast expression systems, may be preferred. Many vectors and host cells are known in the art for such purposes. It is well within the skill of the art to select an appropriate set for the desired application.

[0095] DNA sequences encoding transferrin, portions of transferrin and therapeutic proteins of interest may be cloned from a variety of genomic or cDNA libraries known in the art. The techniques for isolating such DNA sequences using probe-based methods are conventional techniques and are well known to those skilled in the art. Probes for isolating such DNA sequences may be based on published DNA or protein sequences (see, for example, Baldwin, G. S. (1993) Comparison of Transferrin Sequences from Different Species. Comp. Biochem. Physiol. 106B/1:203-218 and all references cited therein, which are hereby incorporated by reference in their entirety). Alternatively, the polymerase chain reaction (PCR) method disclosed by Mullis et al. (U.S. Pat. No. 4,683,195) and Mullis (U.S. Pat. No. 4,683,202), incorporated herein by reference may be used. The choice of library and selection of probes for the isolation of such DNA sequences is within the level of ordinary skill in the art.

[0096] As known in the art “similarity” between two polynucleotides or polypeptides is determined by comparing the nucleotide or amino acid sequence and its conserved nucleotide or amino acid substitutes of one polynucleotide or polypeptide to the sequence of a second polynucleotide or polypeptide. Also known in the art is “identity” which means the degree of sequence relatedness between two polypeptide or two polynucleotide sequences as determined by the identity of the match between two strings of such sequences. Both identity and similarity can be readily calculated (Computational Molecular Biology, Lesk, A. M., ed., Oxford University Press, New York, 1988; Biocomputing: Informatics and Genome Projects, Smith, D. W., ed., Academic Press, New York, 1993; Computer Analysis of Sequence Data, Part I, Griffin, A. M., and Griffin, H. G., eds., Humana Press, New Jersey, 1994; Sequence Analysis in Molecular Biology, von Heinje, G., Academic Press, 1987; and Sequence Analysis Primer, Gribskov, M. and Devereux, J., eds., M Stockton Press, New York, 1991).

[0097] While there exist a number of methods to measure identity and similarity between two polynucleotide or polypeptide sequences, the terms “identity” and “similarity” are well known to skilled artisans (Sequence Analysis in Molecular Biology, von Heinje, G., Academic Press, 1987; Sequence Analysis Primer, Gribskov, M. and Devereux, J., eds., M Stockton Press, New York, 1991; and Carillo, H., and Lipman, D., SIAM J. Applied Math., 48: 1073 (1988). Methods commonly employed to determine identity or similarity between two sequences include, but are not limited to those disclosed in Guide to Huge Computers, Martin J. Bishop, ed., Academic Press, San Diego, 1994, and Carillo, H., and Lipman, D., SIAM J. Applied Math. 48:1073 (1988).

[0098] Preferred methods to determine identity are designed to give the largest match between the two sequences tested. Methods to determine identity and similarity are codified in computer programs. Preferred computer program methods to determine identity and similarity between two sequences include, but are not limited to, GCG program package (Devereux, et al., Nucleic Acids Research 12(1):387 (1984)), BLASTP, BLASTN, FASTA (Atschul, et al., J. Molec. Biol. 215:403 (1990)). The degree of similarity or identity referred to above is determined as the degree of identity between the two sequences indicating a derivation of the first sequence from the second. The degree of identity between two nucleic acid sequences may be determined by means of computer programs known in the art such as GAP provided in the GCG program package (Needleman and Wunsch (1970) Journal of Molecular Biology 48:443-453). For purposes of determining the degree of identity between two nucleic acid sequences for the present invention, GAP is used with the following settings: GAP creation penalty of 5.0 and GAP extension penalty of 0.3.

[0099] Codon Optimization

[0100] The degeneracy of the genetic code permits variations of the nucleotide sequence of a transferrin protein and/or therapeutic protein of interest, while still producing a polypeptide having the identical amino acid sequence as the polypeptide encoded by the native DNA sequence. The procedure, known as “codon optimization” (described in U.S. Pat. No. 5,547,871 which is incorporated herein by reference in its entirety) provides one with a means of designing such an altered DNA sequence. The design of codon optimized genes should take into account a variety of factors, including the frequency of codon usage in an organism, nearest neighbor frequencies, RNA stability, the potential for secondary structure formation, the route of synthesis and the intended future DNA manipulations of that gene. In particular, available methods may be used to alter the codons encoding a given fusion protein with those most readily recognized by yeast when yeast expression systems are used.

[0101] The degeneracy of the genetic code permits the same amino acid sequence to be encoded and translated in many different ways. For example, leucine, serine and arginine are each encoded by six different codons, while valine, proline, threonine, alanine and glycine are each encoded by four different codons. However, the frequency of use of such synonymous codons varies from genome to genome among eukaryotes and prokaryotes. For example, synonymous codon-choice patterns among mammals are very similar, while evolutionarily distant organisms such as yeast (S. cerevisiae), bacteria (such as E. coli) and insects (such as D. melanogaster) reveal a clearly different pattern of genomic codon use frequencies (Grantham, R., et al., Nucl. Acids Res., 8, 49-62 (1980); Grantham, R., et al., Nucl. Acids Res., 9, 43-74 (1981); Maroyama, T., et al., Nucl. Acids Res., 14, 151-197 (1986); Aota, S., et al., Nucl. Acids Res., 16, 315-402 (1988); Wada, K., et al., Nucl. Acids Res., 19 Supp., 1981-1985 (1991); Kurland, C. G., FEBS Letters, 285, 165-169 (1991)). These differences in codon-choice patterns appear to contribute to the overall expression levels of individual genes by modulating peptide elongation rates. (Kurland, C. G., FEBS Letters, 285, 165-169 (1991); Pedersen, S., EMBO J., 3, 2895-2898 (1984); Sorensen, M. A., J. Mol. Biol., 207, 365-377 (1989); Randall, L. L., et al., Eur. J. Biochem., 107, 375-379 (1980); Curran, J. F., and Yarus, M., J. Mol. Biol., 209, 65-77 (1989); Varenne, S., et al., J. Mol, Biol., 180, 549-576 (1984), Varenne, S., et al., J. Mol, Biol., 180, 549-576 (1984); Garel, J. -P., J. Theor. Biol., 43, 211-225 (1974); Ikemura, T., J. Mol. Biol., 146, 1-21 (1981); Ikemura, T., J. Mol. Biol., 151, 389-409 (1981)).

[0102] The preferred codon usage frequencies for a synthetic gene should reflect the codon usages of nuclear genes derived from the exact (or as closely related as possible) genome of the cell/organism that is intended to be used for recombinant protein expression, particularly that of yeast species. As discussed above, in one preferred embodiment the human Tf sequence is codon optimized, before or after modification as herein described for yeast expression as may be the therapeutic protein nucleotide sequence(s).

[0103] Vectors

[0104] Expression units for use in the present invention will generally comprise the following elements, operably linked in a 5′ to 3′ orientation: a transcriptional promoter, a secretory signal sequence, a DNA sequence encoding a modified Tf fusion protein comprising transferrin protein or a portion of a transferrin protein joined to a DNA sequence encoding a therapeutic protein or peptide of interest and a transcriptional terminator. As discussed above, any arrangement of the therapeutic protein or peptide fused to or within the Tf portion may be used in the vectors of the invention. The selection of suitable promoters, signal sequences and terminators will be determined by the selected host cell and will be evident to one skilled in the art and are discussed more specifically below.

[0105] Suitable yeast vectors for use in the present invention are described in U.S. Pat. No. 6,291,212 and include YRp7 (Struhl et al., Proc. Natl. Acad. Sci. USA 76: 1035-1039, 1978), YEpl3 (Broach et al., Gene 8: 121-133, 1979), pJDB249 and pJDB219 (Beggs, Nature 275:104-108, 1978), pPPC0005, pSeCHSA, pScNHSA, pC4 and derivatives thereof. Useful yeast plasmid vectors also include pRS403-406, pRS413-416 and the Pichia vectors available from Stratagene Cloning Systems, La Jolla, Calif. 92037, USA. Plasmids pRS403, pRS404, pRS405 and pRS406 are Yeast Integrating plasmids (YIps) and incorporate the yeast selectable markers HIS3, 7RPI, LEU2 and URA3. PlasmidspRS413˜41.6 are Yeast Centromere plasmids (Ycps).

[0106] Such vectors will generally include a selectable marker, which may be one of any number of genes that exhibit a dominant phenotype for which a phenotypic assay exists to enable transformants to be selected. Preferred selectable markers are those that complement host cell auxotrophy, provide antibiotic resistance or enable a cell to utilize specific carbon sources, and include LEU2 (Broach et al. ibid.), URA3 (Botstein et al., Gene 8: 17, 1979), HIS3(Struhl et al., ibid.) or POTI (Kawasaki and Bell, EP 171,142). Other suitable selectable markers include the CAT gene, which confers chloramphenicol resistance on yeast cells. Preferred promoters for use in yeast include promoters from yeast glycolytic genes (Hitzeman et al., J. Biol. Chem. 225: 12073-12080, 1980; Alber and Kawasaki, J. Mol. Appl. Genet. 1: 419-434, 1982; Kawasaki, U.S. Pat. No. 4,599,311) or alcohol dehydrogenase genes (Young et al., in Genetic Engineering of Microorganisms for Chemicals, Hollaender et al., (eds.), p. 355, Plenum, N.Y., 1982; Ammerer, Meth. Enzymol. 101: 192-201, 1983). In this regard, particularly preferred promoters are the TPI1 promoter (Kawasaki, U.S. Pat. No. 4,599,311) and the ADH2-4.sup.C [see U.S. Pat. No. 6,291,212] promoter (Russell et al., Nature 304: 652-654, 1983). The expression units may also include a transcriptional terminator. A preferred transcriptional terminator is the TPI1 terminator (Alber and Kawasaki, ibid.).

[0107] In addition to yeast, modified fusion proteins of the present invention can be expressed in filamentous fungi, for example, strains of the fungi Aspergillus. Examples of useful promoters include those derived from Aspergillus nidulans glycolytic genes, such as the ADH3 promoter (McKnight et al., EMBO J. 4: 2093-2099, 1985) and the tpiA promoter. An example of a suitable terminator is the ADH3 terminator (McKnight et al., ibid.). The expression units utilizing such components may be cloned into vectors that are capable of insertion into the chromosomal DNA of Aspergillus, for example.

[0108] Mammalian expression vectors for use in carrying out the present invention will include a promoter capable of directing the transcription of the modified Tf fusion protein. Preferred promoters include viral promoters and cellular promoters. Preferred viral promoters include the major late promoter from adenovirus 2 (Kaufman and Sharp, Mol. Cell. Biol. 2: 1304-13199, 1982) and the SV40 promoter (Subramani et al., Mol. Cell. Biol. 1: 854-864, 1981). Preferred cellular promoters include the mouse metallothionein 1 promoter (Palmiter et al., Science 222: 809-814, 1983) and a mouse V.sub.kappa. [see U.S. Pat. No. 6,291,212] promoter (Grant et al., Nuc. Acids Res. 15: 5496, 1987). A particularly preferred promoter is a mouse V.sub.H [see U.S. Pat. No. 6,291,212] promoter (Loh et al., ibid.). Such expression vectors may also contain a set of RNA splice sites located downstream from the promoter and upstream from the DNA sequence encoding the transferrin fusion protein. Preferred RNA splice sites may be obtained from adenovirus and/or immunoglobulin genes.

[0109] Also contained in the expression vectors is a polyadenylation signal located downstream of the coding sequence of interest. Polyadenylation signals include the early or late polyadenylation signals from SV40 (Kaufman and Sharp, ibid.), the polyadenylation signal from the adenovirus 5 E1B region and the human growth hormone gene terminator (DeNoto et al., Nuc. Acids Res. 9: 3719-3730, 1981). A particularly preferred polyadenylation signal is the V.sub.H [see U.S. Pat. No. 6,291,212] gene terminator (Loh et al., ibid.). The expression vectors may include a noncoding viral leader sequence, such as the adenovirus 2 tripartite leader, located between the promoter and the RNA splice sites. Preferred vectors may also include enhancer sequences, such as the SV40 enhancer and the mouse .mu. [see U.S. Pat. No. 6,291,212] enhancer (Gillies, Cell 33: 717-728, 1983). Expression vectors may also include sequences encoding the adenovirus VA RNAs.

[0110] Transformation

[0111] Techniques for transforming fungi are well known in the literature, and have been described, for instance, by Beggs (ibid.), Hinnen et al. (Proc. Natl. Acad. Sci. USA 75: 1929-1933, 1978), Yelton et al., (Proc. Natl. Acad. Sci. USA 81: 1740-1747, 1984), and Russell (Nature 301: 167-169, 1983). The genotype of the host cell will generally contain a genetic defect that is complemented by the selectable marker present on the expression vector. Choice of a particular host and selectable marker is well within the level of ordinary skill in the art.

[0112] Cloned DNA sequences comprising modified Tf fusion proteins of the invention may be introduced into cultured mammalian cells by, for example, calcium phosphate-mediated transfection (Wigler et al., Cell 14: 725, 1978; Corsaro and Pearson, Somatic Cell Genetics 7: 603, 1981; Graham and Van der Eb, Virology 52: 456, 1973.) Other techniques for introducing cloned DNA sequences into mammalian cells, such as electroporation (Neumann et al., EMBO J. 1: 841-845, 1982), or lipofection may also be used. In order to identify cells that have integrated the cloned DNA, a selectable marker is generally introduced into the cells along with the gene or cDNA of interest. Preferred selectable markers for use in cultured mammalian cells include genes that confer resistance to drugs, such as neomycin, hygromycin, and methotrexate. The selectable marker may be an amplifiable selectable marker. A preferred amplifiable selectable marker is the DHFR gene. A particularly preferred amplifiable marker is the DHFR.sup.r [see U.S. Pat. No. 6,291,212] cDNA (Simonsen and Levinson, Proc. Natl. Adac. Sci. USA 80: 2495-2499, 1983). Selectable markers are reviewed by Thilly (Mammalian Cell Technology, Butterworth Publishers, Stoneham, Mass.) and the choice of selectable markers is well within the level of ordinary skill in the art.

[0113] Host Cells

[0114] The present invention also includes a cell, preferably a yeast cell transformed to express a modified transferrin fusion protein of the invention. In addition to the transformed host cells themselves, the present invention also includes a culture of those cells, preferably a monoclonal (clonally homogeneous) culture, or a culture derived from a monoclonal culture, in a nutrient medium. If the polypeptide is secreted, the medium will contain the polypeptide, with the cells, or without the cells if they have been filtered or centrifuged away.

[0115] Host cells for use in practicing the present invention include eukaryotic cells, and in some cases prokaryotic cells, capable of being transformed or transfected with exogenous DNA and grown in culture, such as cultured mammalian, insect, fungal, plant and bacterial cells.

[0116] Fungal cells, including species of yeast (e.g., Saccharomyces spp., Schizosaccharomyces spp., Pichia spp.) may be used as host cells within the present invention. Exemplary genera of yeast contemplated to be useful in the practice, of the present invention as hosts for expressing the, transferrin fusion protein of the inventions are Pichia (formerly classified as Hansenula), Saccharomyces, Kluyveromyces, Aspergillus, Candida, Torulopsis, Torulaspora, Schizosaccharomyces, Citeromyces, Pachysolen, Zygosaecharomyces, Debaromyces, Trichoderma, Cephalosporium, Humicola, Mucor, Neurospora, Yarrowia, Metschunikowia, Rhodosporidium, Leucosporidium, Botryoascus, Sporidiobolus, Endomycopyis, and the like. Examples of Saccharomyces spp. are S. cerevisiae, S. italicus and S. rouxii. Examples of Kiuyveromyces spp. are K. ftagilis, K. lactis and K. marxianus. A suitable Tórulasppra species is T. delbrueckii. Examples of Pichia (Hansenula) spp. are P. angusta (formerly H. polymorpha), P. anomala (formerly H. anomala) and P. pastoris.

[0117] Particularly useful host cells to produce the Tf fusion proteins of the invention are the methanoltrophic Pichia pastoris (Steinlein et al. (1995) Protein Express. Purif 6:619-624). Pichia pastoris has been developed to be an outstanding host for the production of foreign proteins since its alcohol oxidase promoter was isolated and cloned; its transformation was first reported in 1985. P. pastoris can utilize methanol as a carbon source in the absence of glucose. The P. pastoris expression system can use the methanol-induced alcohol oxidase (AOX1) promoter, which controls the gene that codes for the expression of alcohol oxidase, the enzyme which catalyzes the first step in the metabolism of methanol. This promoter has been characterized and incorporated into a series of P. pastoris expression vectors. Since the proteins produced in P. pastoris are typically folded correctly and secreted into the medium, the fermentation of genetically engineered P. pastoris provides an excellent alternative to E. coli expression systems. A number of proteins have been produced using this system, including tetanus toxin fragment, Bordatella pertussis pertactin, human serum albumin and lysozyme.

[0118] The transformation of F. oxysporum may, for instance, be carried out as described by Malardier et al. (1989) Gene 78:147-156.

[0119] Strains of the yeast Saccharomyces cerevisiae are another preferred host. In a preferred embodiment, a yeast cell, or more specifically, a Saccharomyces cerevisiae host cell that contains a genetic deficiency in a gene required for asparagine-linked glycosylation of glycoproteins is used. S. cerevisiae host cells having such defects may be prepared using standard techniques of mutation and selection, although many available yeast strains have been modified to prevent or reduce glycosylation or hypermannosylation. Ballou et al. (J. Biol. Chem. 255: 5986-5991, 1980) have described the isolation of mannoprotein biosynthesis mutants that are defective in genes which affect asparagine-linked glycosylation.

[0120] To optimize production of the heterologous proteins, it is also preferred that the host strain carries a mutation, such as the S. cerevisiae pep4 mutation (Jones, Genetics 85: 23-33, 1977), which results in reduced proteolytic activity. Host strains containing mutations in other protease encoding regions are particularly useful to produce large quantities of the Tf fusion proteins of the invention.

[0121] Host cells containing DNA constructs of the present invention are grown in an appropriate growth medium. As used herein, the term “appropriate growth medium” means a medium containing nutrients required for the growth of cells. Nutrients required for cell growth may include a carbon source, a nitrogen source, essential amino acids, vitamins, minerals and growth factors. The growth medium will generally select for cells containing the DNA construct by, for example, drug selection or deficiency in an essential nutrient which are complemented by the selectable marker on the DNA construct or co-transfected with the DNA construct. Yeast cells, for example, are preferably grown in a chemically defined medium, comprising a non-amino acid nitrogen source, inorganic salts, vitamins and essential amino acid supplements. The pH of the medium is preferably maintained at a pH greater than 2 and less than 8, preferably at pH 6.5. Methods for maintaining a stable pH include buffering and constant pH control, preferably through the addition of sodium hydroxide. Preferred buffering agents include succinic acid and Bis-Tris (Sigma Chemical Co., St. Louis, Mo.). Yeast cells having a defect in a gene required for asparagine-linked glycosylation are preferably grown in a medium containing an osmotic stabilizer. A preferred osmotic stabilizer is sorbitol supplemented into the medium at a concentration between 0.1 M and 1.5 M., preferably at 0.5 M or 1.0 M.

[0122] Cultured mammalian cells are generally grown in commercially available serum-containing or serum-free media. Selection of a medium appropriate for the particular cell line used is within the level of ordinary skill in the art. Transfected mammalian cells are allowed to grow for a period of time, typically 1-2 days, to begin expressing the DNA sequence(s) of interest. Drug selection is then applied to select for growth of cells that are expressing the selectable marker in a stable fashion. For cells that have been transfected with an amplifiable selectable marker the drug concentration may be increased in a stepwise manner to select for increased copy number of the cloned sequences, thereby increasing expression levels.

[0123] Baculovirus/insect cell expression systems may also be used to produce the modified Tf fusion proteins of the invention. The BacPAK™ Baculovirus Expression System (BD Biosciences (Clontech) expresses recombinant proteins at high levels in insect host cells. The target gene is inserted into a transfer vector, which is cotransfected into insect host cells with the linearized BacPAK6 viral DNA. The BacPAK6 DNA is missing an essential portion of the baculovirus genome. When the DNA recombines with the vector, the essential element is restored and the target gene is transferred to the baculovirus genome. Following recombination, a few viral plaques are picked and purified, and the recombinant phenotype is verified. The newly isolated recombinant virus can then be amplified and used to infect insect cell cultures to produce large amounts of the desired protein.

[0124] Secretory Signal Sequences

[0125] The terms “secretory signal sequence” or “signal sequence” or “secretion leader sequence” are used interchangeably and are described, for example in U.S. Pat. No. 6,291,212 and U.S. Pat 5,547,871, both of which are herein incorporated by reference in their entirety. Secretory signal sequences or signal sequences or secretion leader sequences encode secretory peptides. A secretory peptide is an amino acid sequence that acts to direct the secretion of a mature polypeptide or protein from a cell. Secretory peptides are generally characterized by a core of hydrophobic amino acids and are typically (but not exclusively) found at the amino termini of newly synthesized proteins. Very often the secretory peptide is cleaved from the mature protein during secretion. Secretory peptides may contain processing sites that allow cleavage of the signal peptide from the mature protein as it passes through the secretory pathway. Processing sites may be encoded within the signal peptide or may be added to the signal peptide by, for example, in vitro mutagenesis.

[0126] Secretory peptides may be used to direct the secretion of modified Tf fusion proteins of the invention. One such secretary peptide that may be used in combination with other secretory peptides is the third domain of the yeast Barrier protein. Secretory signal sequences or signal sequences or secretion leader sequences are required for a complex series of post-translational processing steps which result in secretion of a protein. If an intact signal sequence is present, the protein being expressed enters the lumen of the rough endoplasmic reticulum and is then transported through the Golgi apparatus to secretory vesicles and is finally transported out of the cell. Generally, the signal sequence immediately follows the initiation codon and encodes a signal peptide at the amino-terminal end of the protein to be secreted. In most cases, the signal sequence is cleaved off by a specific protease, called a signal peptidase. Preferred signal sequences improve the processing and export efficiency of recombinant protein expression using viral, mammalian or yeast expression vectors. In some cases, the native Tf signal sequence may be used to express and secrete fusion proteins of the invention.

[0127] Linkers

[0128] The Tf moiety and therapeutic protein moiety(s) of the modified transferrin fusion proteins of the invention can be fused directly or using a linker peptide of various lengths to provide greater physical separation and allow more spatial mobility between the fused proteins and thus maximize the accessibility of the therapeutic protein portion, for instance, for binding to its cognate receptor. The linker peptide may consist of amino acids that are flexible or more rigid. For example, a linker such as but not limited to a poly-glycine stretch. The linker can be less than about 50, 40, 30, 20, or 10 amino acid residues. The linker can be covalently linked to and between the transferrin protein or portion thereof and the therapeutic protein.

[0129] Detection of Tf Fusion Proteins

[0130] Assays for detection of biologically active modified transferrin-therapeutic protein fusions may include Western transfer, protein blot or colony filter as well as activity based assays that detect the fused therapeutic protein. A Western transfer filter may be prepared using the method described by Towbin et al. (Proc. Natl. Acad. Sci. USA 76: 4350-4354, 1979). Briefly, samples are electrophoresed in a sodium dodecylsulfate polyacrylamide gel. The proteins in the gel are electrophoretically transferred to nitrocellulose paper. Protein blot filters may be prepared by filtering supernatant samples or concentrates through nitrocellulose filters using, for example, a Minifold (Schleicher & Schuell, Keene, N. H.). Colony filters may be prepared by growing colonies on a nitrocellulose filter that has been laid across an appropriate growth medium. In this method, a solid medium is preferred. The cells are allowed to grow on the filters for at least 12 hours. The cells are removed from the filters by washing with an appropriate buffer that does not remove the proteins bound to the filters. A preferred buffer comprises 25 mM Tris-base, 19 mM glycine, pH 8.3, 20% methanol.

[0131] Fusion proteins of the invention may also be detected by assaying for the activity of the therapeutic protein moiety. Such assays are readily available, including but not limited to, those assays described in Table 1. Specifically, transferrin fusion proteins of the invention may be assayed for functional activity (e.g., biological activity or therapeutic activity) using the assay referenced in the “Exemplary Activity Assay” column of Table 1. Additionally, one of skill in the art may routinely assay fragments of a therapeutic protein corresponding to a therapeutic protein portion of a fusion protein of the invention, for activity using assays referenced in its corresponding row of Table 1. Further, one of skill in the art may routinely assay fragments of a modified transferrin protein for activity using assays known in the art.

[0132] For example, in one embodiment where one is assaying for the ability of a transferrin fusion protein of the invention to bind or compete with a therapeutic protein for binding to an anti-therapeutic polypeptide antibody and/or anti-transferrin antibody, various immunoassays known in the art can be, used, including but not limited to, competitive and non-competitive assay systems using techniques such as radioimmunoassays, ELISA (enzyme linked immunosorbent assay), sandwich immunoassays, immunoradiometric assays, gel diffusion precipitation reactions, immunodiffusion assays, in situ immunoassays (using colloidal gold, enzyme or radioisotope labels, for example), western blots, precipitation reactions, agglutination assays (e.g., gel agglutination assays), complement fixation assays, immunofluorescence assays, protein A assays, and immunoelectrophoresis assays, etc. In one embodiment, antibody binding is detected by detecting a label on the primary antibody. In another embodiment, the primary antibody is detected by detecting binding of a secondary antibody or reagent to the primary antibody. In a further embodiment, the secondary antibody is labeled. Many means are known in the art for detecting binding in an immunoassay and are within the scope of the present invention.

[0133] In a further embodiment, where a binding partner (e.g., a receptor or a ligand) of a therapeutic protein is identified, binding to that binding partner by a transferrin fusion protein containing that therapeutic protein as the therapeutic protein portion of the fusion can be assayed, e.g., by means well-known in the art, such as, for example, reducing and non-reducing gel chromatography, protein affinity chromatography, and affinity blotting. Other methods will be known to the skilled artisan and are within the scope of the invention.

[0134] Isolation/Purification of Modified Transferrin Fusion Proteins

[0135] Secreted, biologically active, modified transferrin fusion proteins may be isolated from the medium of host cells grown under conditions that allow the secretion of the biologically active fusion proteins. The cell material is removed from the culture medium, and the biologically active fusion proteins are isolated using isolation techniques known in the art. Suitable isolation techniques include precipitation and fractionation by a variety of chromatographic methods, including gel filtration, ion exchange chromatography and affinity chromatography.

[0136] A particularly preferred purification method is affinity chromatography on an iron binding or metal chelating column or an immunoaffinity chromatography using an antibody directed against the transferrin or therapeutic protein or peptide portion of the polypeptide fusion. The antibody is preferably immobilized or attached to a solid support or substrate. A particularly preferred substrate is CNBr-activated Sepharose (Pharmacia LKB Technologies, Inc., Piscataway, N.J.). By this method, the medium is combined with the antibody/substrate under conditions that will allow binding to occur. The complex may be washed to remove unbound material, and the transferrin fusion protein is released or eluted through the use of conditions unfavorable to complex formation. Particularly useful methods of elution include changes in pH, wherein the immobilized antibody has a high affinity for the ligand at a first pH and a reduced affinity at a second (higher or lower) pH; changes in concentration of certain chaotropic agents; or through the use of detergents.

[0137] Labeled Modified Transferrin Fusion Proteins

[0138] Transferrin fusion proteins of the present invention may also be labeled with a radioisotope or other imaging agent and used for in vivo diagnostic purposes. Preferred radioisotope imaging agents include iodine-125 and technetium-99, with technetium-99 being particularly preferred. Methods for producing protein-isotope conjugates are well known in the art, and are described by, for example, Eckelman et al. (U.S. Pat. No. 4,652,440), Parker et al. (WO 87/05030) and Wilber et al. (EP 203,764). Alternatively, the transferrin fusion proteins may be bound to spin label enhancers and used for magnetic resonance (MR) imaging. Suitable spin label enhancers include stable, sterically hindered, free radical compounds such as nitroxides. Methods for labeling ligands for MR imaging are disclosed by, for example, Coffman et al. (U.S. Pat. No. 4,656,026). For administration, the labeled transferrin fusion proteins are combined with a pharmaceutically acceptable carrier or diluent, such as sterile saline or sterile water. Administration is preferably by bolus injection, preferably intravenously.

[0139] Production of Fusion Proteins

[0140] The present invention further provides methods for producing a modified fusion protein of the invention using nucleic acid molecules herein described. In general terms, the production of a recombinant form of a protein typically involves the following steps.

[0141] A nucleic acid molecule is first obtained that encodes a transferrin fusion protein of the invention. The nucleic acid molecule is then preferably placed in operable linkage with suitable control sequences, as described above, to form an expression unit containing the protein open reading frame. The expression unit is used to transform a suitable host and the transformed host is cultured under conditions that allow the production of the recombinant protein. Optionally the recombinant protein is isolated from the medium or from the cells; recovery and purification of the protein may not be necessary in some instances where some impurities may be tolerated.

[0142] Each of the foregoing steps can be accomplished in a variety of ways. For example, the construction of expression vectors that are operable in a variety of hosts is accomplished using appropriate replicons and control sequences, as set forth above. The control sequences, expression vectors, and transformation methods are dependent on the type of host cell used to express the gene and were discussed in detail earlier and are otherwise known to persons skilled in the art. Suitable restriction sites can, if not normally available, be added to the ends of the coding sequence so as to provide an excisable gene to insert into these vectors. A skilled artisan can readily adapt any host/expression system known in the art for use with the nucleic acid molecules of the invention to produce a desired recombinant protein.

[0143] As discussed above, any expression system may be used, including yeast, bacterial, animal, plant, eukaryotic and prokaryotic systems. In some embodiments, yeast, mammalian cell culture and transgenic animal or plant production systems are preferred. In other embodiments, yeast systems that have been modified to reduce native yeast glycosylation, hyper-glycosylation or proteolytic activity may be used.

[0144] Therapeutic Molecules

[0145] Any therapeutic molecule may be used as the fusion partner to Tf according to the methods and compositions of the present invention. As used herein, a therapeutic molecule is typically a protein or peptide capable of exerting a beneficial biological effect in vitro or in vivo and includes proteins or peptides that exert a beneficial effect in relation to normal homeostasis, physiology or a disease state. Therapeutic molecules do not include, fusion partners commonly used as markers or protein purification aids, such as galactosidases (see for example, U.S. Pat. No. 5,986,067 and Aldred et al. (1984) Biochem. Biophys. Res. Commun. 122: 960-965). For instance, a beneficial effect as related to a disease state includes any effect that is advantageous to the treated subject, including disease prevention, disease stabilization, the lessening or alleviation of disease symptoms or a modulation, alleviation or cure of the underlying defect to produce an effect beneficial to the treated subject.

[0146] A modified transferrin fusion protein of the invention includes at least a fragment or variant of a therapeutic protein and at least a fragment or variant of modified serum transferrin, which are associated with one another, preferably by genetic fusion or chemical conjugation.

[0147] In one embodiment, the transferrin fusion protein includes a modified transferrin molecule linked to a neuropharmaceutical agent. In another embodiment, the modified transferrin fusion protein includes transferrin at the carboxyl terminus linked to a neuropharmaceutical agent at the amino terminus. In an alternate embodiment, the modified transferrin fusion protein includes transferrin at the amino terminus linked to a neuropharmaceutical agent at the carboxy terminus. In specific embodiments, the neuropharmaceutical agent is either nerve growth factor or ciliary neurotrophic factor.

[0148] In further embodiments, a modified transferrin fusion protein of the invention may contain at least a fragment or variant of a therapeutic protein, and/or at least a fragment or variant of an antibody. In a further embodiment, the transferrin fusion proteins can contain peptide fragments or peptide variants of proteins or antibodies wherein the variant or fragment retains at least one biological or therapeutic activity. The transferrin fusion proteins can contain therapeutic proteins that can be peptide fragments or peptide variants at least about 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 20, at least 25, at least 30, at least 35, or at least about 40, at least about 50, at least about 55, at least about 60 or at least about 70 or more amino acids in length fused to the N and/or C termini, inserted within, or inserted into a loop of a modified transferrin.

[0149] In another embodiment, the modified transferrin fusion molecules contain a therapeutic protein portion that can be fragments of a therapeutic protein that include the full length protein as well as polypeptides having one or more residues deleted from the amino terminus of the amino acid sequence.

[0150] In another embodiment, the modified transferrin fusion molecules contain a therapeutic protein portion that can be fragments of a therapeutic protein that include the full length protein as well as polypeptides having one or more residues deleted from the carboxy terminus of the amino acid sequence.

[0151] In another embodiment, the modified transferrin fusion molecules contain a therapeutic protein portion that can have one or more amino acids deleted from both the amino and the carboxy termini.

[0152] In another embodiment, the modified transferrin fusion molecules contain a therapeutic protein portion that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% identical to a reference therapeutic protein set forth herein, or fragments thereof. In further embodiments, the transferrin fusion molecules contain a therapeutic protein portion that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% identical to reference polypeptides having the amino acid sequence of N- and C-terminal deletions as described above.

[0153] In another embodiment, the modified transferrin fusion molecules contain the therapeutic protein portion that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100%, identical to, for example, the native or wild-type amino acid sequence of a therapeutic protein. Fragments, of these polypeptides are also provided.

[0154] The therapeutic proteins corresponding to a therapeutic protein portion of a modified transferrin fusion protein of the invention, such as cell surface and secretory proteins, can be modified by the attachment, of one or more oligosaccharide groups. The modification referred to as glycosylation, can significantly affect the physical properties of proteins and can be important in protein stability, secretion, and localization. Glycosylation occurs at specific locations along the polypeptide backbone. There are usually two major types of glycosylation: glycosylation characterized by O-linked oligosaccharides, which are attached to serine or threonine residues; and glycosylation characterized by N-linked oligosaceharides, which are attached to asparagine residues in an Asn-X-Ser/Thr sequence, where X can be an amino add except proline. Variables such as protein structure and cell type influence the number and nature of the carbohydrate units within the chains at different glycosylation sites. Glycosylation isomers are also common at the same site within a given cell type. For example, several types of human interferon are glycosylated.

[0155] Therapeutic proteins corresponding to a therapeutic protein portion of a transferrin fusion protein of the invention, as well as analogs and variants thereof, may be modified so that glycosylation at one or more sites is altered as a result of manipulation(s) of their nucleic acid sequence by the host cell in which they are expressed, or due to other conditions of their expression. For example, glycosylation isomers may be produced by abolishing or introducing glycosylation sites, e.g., by substitution or deletion of amino acid residues, such as substitution of glutamine for asparagine, or unglycosylated recombinant proteins may be produced by expressing the proteins in host cells that will not glycosylate them, e.g. in glycosylation-deficient yeast. These approaches are known in the art.

[0156] Therapeutic proteins and their nucleic acid sequences are well known in the art and available in public databases such as Chemical Abstracts Services Databases (e.g., the CAS Registry), GenBank, and GenSeq. The Accession Numbers and sequences referred to below are herein incorporated by reference in their entirety.

[0157] In other embodiments, the transferrin fusion proteins of the invention are capable of a therapeutic activity and/or biologic activity, corresponding to the therapeutic activity and/or biologic activity of the therapeutic protein listed in the corresponding row of Table 1 and elsewhere in this application. (See, e.g., the “Biological Activity” and “Therapeutic Protein X” columns of Table 1.) In further embodiments, the therapeutically active protein portions of the transferrin fusion proteins of the invention are fragments or variants of the reference sequences cited herein.

[0158] The present invention is further directed to modified Tf fusion proteins comprising fragments of the therapeutic proteins herein described. Even if deletion of one or more amino acids from the N-terminus of a protein results in modification or loss of one or more biological functions of the therapeutic protein portion, other therapeutic activities and/or functional activities (e.g., biological activities, ability to multimerize, ability to bind a ligand) may still be retained. For example, the ability of polypeptides with N-terminal deletions to induce and/or bind to antibodies which recognize the complete or mature forms of the polypeptides generally will be retained with less than the majority of the residues of the complete polypeptide removed from the N-terminus. Whether a particular polypeptide lacking N-terminal residues of a complete polypeptide retains such immunologic activities can be assayed by routine methods described herein and otherwise known in the art. It is not unlikely that a mutant with a large number of deleted N-terminal amino acid residues may retain some biological or immunogenic activities. In fact, peptides composed of as few as six amino acid residues may often evoke an immune response.

[0159] Also as mentioned above, even if deletion of one or more amino acids from the N-terminus or C-terminus of a therapeutic protein results in modification or loss of one or more biological functions of the protein, other functional activities (e.g., biological activities, ability to multimerize, ability to bind a ligand) and/or therapeutic activities may still be retained. For example the ability of polypeptides with C-terminal deletions to induce and/or bind to antibodies which recognize the complete or mature forms of the polypeptide generally will be retained when less than the majority of the residues of the complete or mature polypeptide are removed from the C-terminus. Whether a particular polypeptide lacking the N-terminal and/or, C-terminal residues of a reference polypeptide retains therapeutic activity can readily be determined by routine methods described herein and/or otherwise known in the art.

[0160] Peptide fragments of the therapeutic proteins can be fragments comprising, or alternatively, consisting of, an amino acid sequence that displays a therapeutic activity and/or functional activity (e.g. biological activity) of the polypeptide sequence of the therapeutic protein of which the amino acid sequence is a fragment.

[0161] Other polypeptide fragments are biologically active fragments. Biologically active fragments are those exhibiting activity similar, but not necessarily identical, to an activity of a therapeutic protein used in the present invention. The biological activity of the fragments may include an improved desired activity, or a decreased undesirable activity.

[0162] Generally, variants of proteins are overall very similar, and, in many regions, identical to the amino acid sequence of the therapeutic protein corresponding to a therapeutic protein portion of a transferrin fusion protein of the invention. Nucleic acids encoding these variants are also encompassed by the invention.

[0163] Further therapeutic polypeptides that may be used in the invention are polypeptides encoded by polynucleotides which hybridize to the complement of a nucleic acid molecule encoding an amino acid sequence of a therapeutic protein under stringent hybridization conditions which are known to those of skill in the art. (see, for example, Ausubel, F. M. et al., eds., 1989 Current protocol in Molecular Biology, Green Publishing Associates, Inc., and John Wiley & Sons Inc., New. York). Polynucleotides encoding these polypeptides are also encompassed by the invention.

[0164] By a polypeptide-having an amino acid sequence at least, for example, 95% “identical” to a query amino acid sequence of the present invention, it is intended that the amino acid sequence of the subject polypeptide is identical to the query sequence except that the subject polypeptide sequence may include up to five amino acid alterations per each 100 amino acids of the query amino acid sequence. In other words, to obtain a polypeptide having an amino acid sequence at least 95% identical to a query amino acid sequence, up to 5% of the amino acid residues in the subject sequence may be inserted, deleted, or substituted with another amino acid. These alterations of the reference sequence may occur at the amino- or carboxy-terminal positions of the reference amino acid sequence or anywhere between those terminal positions, interspersed either individually among residues in the reference sequence, or in one or more contiguous groups within the reference sequence.

[0165] As a practical matter, whether any particular polypeptide is at least about 80%, 85%, 90%,95%, 96%, 97%, 98% or 99% identical to, for instance, the amino acid sequence of a transferrin fusion protein of the invention or a fragment thereof (such, as the therapeutic protein portion of the transferrin fusion protein or the transferrin portion of the transferrin fusion protein), can be determined conventionally using known computer programs. A preferred method for determining the best overall match between a query sequence (a sequence of the present invention) and a subject sequence, also referred to as a global sequence alignment, can be determined using the FASTDB computer program based on the algorithm of Brufiag-et al. (Comp. App. Biosci 245-(1990)).

[0166] The polynucleotide variants of the invention may contain alterations in the coding regions, non-coding regions, or both. Polynucleotide variants containing alterations which produce silent substitutions, additions, or deletions, but do not alter the properties or activities of the encoded polypeptide may be used to produce modified Tf fusion proteins. Nucleotide variants produced by silent substitutions due to the degeneracy of the genetic code can be utilized. Moreover, polypeptide variants in which less than about 50, less than 40, less than 30, less than 20, less than 10, or 5-50, 5-25, 5-10, 1-5, or 1-2 amino acids are substituted, deleted, or added in any combination can also be utilized. Polynucleotide variants can be produced for a variety of reasons, e.g., to optimize codon expression for a particular host (change codons in the human mRNA to those preferred by a host, such as, yeast or E. coli as described above).

[0167] In other embodiments, the therapeutic protein moiety has conservative substitutions compared to the wild-type sequence. By “conservative substitutions” is intended swaps within groups such as replacement of the aliphatic or hydrophobic amino acids Ala, Val, Leu and Ile; replacement of the hydroxyl residues Ser and Thr; replacement of the acidic residues Asp and Glu; replacement of the amide residues Asn and Gln, replacement of the basic residues Lys, Arg, and His; replacement of the aromatic residues Phe, Tyr, and Trp, and replacement of the small-sized amino acids Ala, Ser, Thr, Met, and Gly. Guidance concerning how to make phenotypically silent amino acid substitutions is provided, for example, in Bowie et al., “Deciphering the Message in Protein Sequences: Tolerance to Amino Acid Substitutions,” Science 247:1306-1310 (1990). In specific embodiments, the polypeptides of the invention comprise, or alternatively, consist of, fragments or variants of the amino acid sequence of a therapeutic protein described herein and/or serum transferrin, and/modified transferrin protein of the invention, wherein the fragments or variants have 1-5,5-10, 5-25, 5-50, 10-50 or 50-150 amino acid residue additions, substitutions, and/or deletions when compared to the reference amino acid sequence. In further embodiments, the amino acid substitutions are conservative. Nucleic acids encoding these polypeptides are also encompassed by the invention.

[0168] The modified fusion proteins of the present invention can be composed of amino-acids joined to each other by peptide bonds or modified peptide bonds and may contain amino acids other than the 20 gene-encoded amino acids. The polypeptides may be modified by either natural processes, such as post-translational processing, or by chemical modification techniques which are well known in the art. Such modifications are well described in basic texts and in more detailed monographs, as well as in a voluminous research literature.

[0169] Modifications can occur anywhere in a polypeptide, including the peptide backbone, the amino acid side-chains and the amino or carboxy termini. It will be appreciated that the same type of modification may be present in the same or varying degrees at several sites in a given polypeptide. Also, a given polypeptide may contain many types of modifications. Polypeptides may be branched, for example, as a result of ubiquitination, and they may be cyclic, with or without branching. Cyclic, branched, and branched cyclic polypeptides may result from posttranslation natural processes or may be made by synthetic methods. Modifications include acetylation, acylation, ADP-ribosylation, amidation, covalent attachment of flavin, covalent attachment of a heme moiety, covalent attachment of a nucleotide or nucleotide derivative, covalent attachment of a lipid or lipid derivative, covalent attachment of phosphotidylinositol, cross-linking, cyclization, disulfide bond formation, demethylation, formation of covalent cross-links, formation of cysteine, glycosylation, GPI anchor formation, hydroxylation, iodination, methylation, myristylation, oxidation, pegylation, proteolytic processing, phosphorylation, prenylation, racemization, sulfation, transfer-RNA mediated addition of amino acids to proteins such as arginylaltion, and ubiquitination. (See, for instance, PROTEINS—STRUCTURE AND MOLECULAR PROPERTIES, 2nd Ed., T. E. Creighton, W. H. Freeman and Company, New York(1993); POST-TRANSLATIONAL COVALENT MODIFICATION OF PROTEINS, B. C. Johnson, Ed., Academic Press, New' York, pgs. 1-12 (1983); Seifter et al. (1990) Meth. Enzymol. 182:626-646; Rattan et al., Ann. N.Y. Acad. Sci. 663:48-62.

[0170] Therapeutic molecules that may be fused to or inserted into Tf include, but are not limited to, hormones, matrix proteins, immunosuppressants, bronchodilators, cardiovascular agents, enzymes, CNS agents, neurotransmitters, receptor proteins or peptides, growth hormones, growth factors, antiviral peptides, fusogenic inhibitor peptides, cytokines, lymphokines, monokines, interleukins, colony stimulating factors, differentiation factors, angiogenic factors, receptor ligands, cancer-associated proteins, antineoplastics, viral peptides, antibiotic peptides, blood proteins, antagonist proteins, transcription factors, anti-angiogenic factors, antagonist proteins or peptides, receptor antagonists, antibodies, single chain antibodies and cell adhesion molecules. Different therapeutic molecules may be combined into a single fusion protein to produce a bi or multi-functional therapeutic molecule. Different molecules may also be used in combination to produce a fusion protein with a therapeutic entity and a targeting entity.

[0171] Cytokines are soluble proteins released by cells of the immune system, which act nonenzymatically through specific receptors to regulate immune responses. Cytokines resemble hormones in that they act at low concentrations bound with high affinity to a specific receptor. The term “cytokine” is used herein to describe naturally occurring or recombinant proteins, analogs thereof, and fragments thereof which elicit a specific biological response in a cell which has a receptor for that cytokine. Cytokines preferably include interleukins such as interleukin-2 (IL-2) (GenBank Acc. No. S77834), IL-3 (GenBank Acc. No. M14743), IL-4 (GenBank Acc. No. M23442), IL-5 (GenBank Acc. No. J03478), IL-6 (GenBank Acc. No. M14584), IL-7 (GenBank Acc. No. NM—000880), IL-10 (GenBank Acc. No. NM—000572), IL-12 (GenBank Acc. No.AF180562 and GenBank Acc. No. AF180563), IL-13 (GenBank Acc. No. U10307), IL-14 (GenBank Acc. No. XM—170924), IL-15 (GenBank Acc. No. X91233), IL-16 (GenBank Acc. No. NM—004513), IL-17 (GenBank Acc. No. NM—002190) and IL-18 (GenBank Acc. No. NM—001562), hematopoietic factors such as granulocyte-macrophage colony stimulating factor (GM-CSF) (GenBank Acc. No. X03021), granulocyte colony stimulating factor (G-CSF) (GenBank Acc. No. X03656), platelet activating factor (GenBank Acc. No. NM—000437) and erythropoeitin (GenBank Acc. No. X02158), tumor necrosis factors (TNF) such as TNFα (GenBank Acc. No. X02910), lymphokines such as lymphotoxin-α (GenBank Acc. No. X02911), lymphotoxin-β (GenBank Acc. No. L11016), leukoregulin, macrophage migration inhibitory factor (GenBank Acc. No. M25639), and neuroleukin (GenBank Acc. No. K03515), regulators of metabolic processes such as leptin (GenBank Acc. No. U43415), interferons such as interferon α (IFNα) (GenBank Acc. No. M54886), IFNβ (GenBank Acc. No. V00534), IFNγ (GenBank Acc. No. J00219), IFNo (GenBank Acc. No. NM—002177), thrombospondin 1 (THBS1) (GenBank Acc. No. NM—003246), THBS2 (GenBank Acc. No. L12350), THBS3 (GenBank Acc. No. L38969), THBS4 (GenBank Acc. No. NM—003248), and chemokines. Preferably, the modified transferrin-cytokine fusion protein of the present invention displays cytokine biological activity.

[0172] The term “hormone” is used herein to describe any one of a number of biologically active substances that are produced by certain cells or tissues and that cause specific biological changes or activities to occur in another cell or tissue located elsewhere in the body. Hormones preferably include proinsulin (GenBank Acc. No. V00565), insulin (GenBank Acc. No. NM—000207), growth hormone 1 (GenBank Acc. No. V00520), growth hormone 2 (GenBank Acc. No. F006060), growth hormone release factor (GenBank Acc. No. NM—021081), insulin-like growth factor I (GenBank Acc. No. M27544), insulin-like growth factor II (GenBank Acc. No. NM—000612), insulin-like growth factor binding protein 1 (IGFBP-1) (GenBank Acc. No. M59316), IGFBP-2 (GenBank Acc. No. X16302), IGFBP-3 (GenBank Acc. No. NM—000598), IGFBP-4 (GenBank Acc. No. Y12508), IGFBP-5 (GenBank Acc. No. M65062), IGFBP-6 (GenBank Acc. No. NM—002178), IGFBP-7 (GenBank Acc. No. NM 001553), chorionic gonadotropin β chain (GenBank Acc. No. NM—033142), chorionic gonadotropin a chain (GenBank Acc. No. NM—000735), luteinizing hormone P (GenBank Acc. No. X00264), follicle-stimulating hormone β (GenBank Acc. No. NM—000510), thyroid-stimulating hormone β (GenBank Acc. No. NM—000549), prolactin (GenBank Acc. No. NM—000948), pro-opiomelanocortin (GenBank Acc. No. V01510), corticotropin (ACTH), β-lipotropin, α-melanocyte stimulating hormone (α-MSH), γ-lipotropin, β-MSH, β-endorphin, and corticotropin-like intermediate lobe peptide (CLIP).

[0173] The term “growth factor” is used herein to describe any protein or peptide that binds to a receptor to stimulate cell proliferation. Growth factors preferably include platelet-derived growth factor-α (PDGF-α) (GenBank Acc. No. X03795), PDGF-β (GenBank Acc. No. X02811), steroid hormones, epidermal growth factor (EGF) (GenBank Acc. No. NM—001963), fibroblast growth factors such as fibroblast growth factor 1 (FGF1) (GenBank Acc. No. NM—000800), FGF2 (GenBank Acc. No. NM—002006), FGF3 (GenBank Acc. No. NM—005247), FGF4 (GenBank Acc. No. NM—002007), FGF5 (GenBank Acc. No. M37825), FGF6 (GenBank Acc. No. X57075), FGF7 (GenBank Acc. No. NM—002009), FGF8 (GenBank Acc. No. AH006649), FGF9 (GenBank Acc. No. NM—002010), FGF10 (GenBank Acc. No. AB002097), FGF11 (GenBank Acc. No. NM—004112), FGF12 (GenBank Acc. No. NM—021032), FGF13 (GenBank Acc. No. NM—004114), FGF14 (GenBank Acc. No. NM—004115), FGF16 (GenBank Acc. No. AB009391), FGF17 (GenBank Acc. No. NM—003867), FGF18 (GenBank Acc. No. AF075292), FGF19 (GenBank Acc. No. NM—005117), FGF20 (GenBank Acc. No. NM—019851), FGF21 (GenBank Acc. No. NM 019113), FGF22 (GenBank Acc. No. NM—020637), and FGF23 (GenBank Acc. No. NM—020638), angiogenin (GenBank Acc. No. M11567), brain-derived neurotrophic factor (GenBank Acc. No. M61176), ciliary neurotrophic growth factor (GenBank Acc. No. X60542), transforming growth factor-α (TGF-α) (GenBank Acc. No. X70340), TGF-β (GenBank Acc. No. X02812), nerve growth factor-α (NGF-α) (GenBank Acc. No. NM—010915), NGF-β (GenBank Acc. No. X52599), tissue inhibitor of metalloproteinase I (TIMPI) (GenBank Acc. No. NM—003254), TIMP2 (GenBank Acc. No. NM—003255), TIMP3 (GenBank Acc. No. U02571), TIMP4 (GenBank Acc. No. U76456) and macrophage stimulating 1 (GenBank Acc. No. L11924).

[0174] The term “matrix protein” is used herein to describe proteins or peptides that are normally found in the extracellular matrix. These proteins may be functionally important for strength, filtration, or adhesion. Matrix proteins preferably include collagens such as collagen I (GenBank Acc. No. Z74615), collagen II (GenBank Acc. No. X16711), collagen III (GenBank Acc. No. X14420), collagen IV (GenBank Acc. No. NM—001845), collagen V (GenBank Acc. No. NM—000393), collagen VI (GenBank Acc. No. NM—058175), collagen VII (GenBank Acc. No. L02870), collagen VIII (GenBank Acc. No. NM—001850), collagen IX (GenBank Acc. No. X54412), collagen X (GenBank Acc. No. X60382), collagen XI (GenBank Acc. No. J04177), and collagen XII (GenBank Acc. No. U73778), laminin proteins such as LAMA2 (GenBank Acc. No. NM—000426), LAMA3 (GenBank Acc. No. L34155), LAMA4 (GenBank Acc. No. NM—002290), LAMBI (GenBank Acc. No. NM—002291), LAMB3 (GenBank Acc. No. L25541), LAMC1 (GenBank Acc. No. NM—002293), nidogen (GenBank Acc. No. NM 002508), α-tectorin (GenBank Acc. No. NM—005422), β-tectorin (GenBank Acc. No. NM—058222), and fibronectin (GenBank Acc. No. X02761).

[0175] The term “blood proteins” are traditionally defined as those sourced from plasma, many now commonly produced by recombinant means, and include, but are not limited to native serum proteins, derivatives, fragments and mutants or variants thereof, blood clotting factors, derivatives, mutants, variants and fragments (including factors VII, VIII, IX, X), protease inhibitors (antithrombin 3, alpha-1 antitrypsin), urokinase-type plasminogen activator, immunoglobulins, von Willebrand factor and von Willebrand mutants, fibronectin, fibrinogen, thrombin and hemoglobin.

[0176] The term “enzyme” is used herein to describe any protein or proteinaccous substance which catalyzes a specific reaction without itself being permanently altered or destroyed. Enzymes preferably include coagulation factors such as F2 (GenBank Acc. No. XM—170688), F7 (GenBank Acc. No. XM—027508), F8 (GenBank Acc. No. XM—013124), F9 (GenBank Acc. No. NM—000133), F10 (GenBank Acc. No. AF503510) and others, matrix metalloproteinases such as matrix metalloproteinase I (GenBank Acc. No. MMP1) (GenBank Acc. No. NM—002421), MMP2 (GenBank Acc. No. NM—004530), MMP3 (GenBank Acc. No. NM—002422), MMP7 (GenBank Acc. No. NM—002423), MMP8 (GenBank Acc. No. NM—002424), MMP9 (GenBank Acc. No. NM—004994), MMP10 (GenBank Acc. No. NM—002425), MMP12 (GenBank Acc. No. NM—002426), MMP13 (GenBank Acc. No. X75308), MMP20 (GenBank Acc. No. NM—004771), adenosine deaminase (GenBank Acc. No. NM—000022), mitogen activated protein kinases such as MAPK3 (GenBank Acc. No. XM—055766), MAP2K2 (GenBank Acc. No. NM—030662), MAP2K1 (GenBank Acc. No. NM—002755), MAP2K4 (GenBank Acc. No. NM—003010), MAP2K7 (AF013588), and MAPK12 (NM—002969), kinases such as JNKK1 (GenBank Acc. No. U17743), JNKK2 (GenBank Acc. No. AF014401), JAKI (M64174), JAK2 (NM—004972), and JAK3 (NM—000215), and phosphatases such as PPM1A (GenBank Acc. No. NM—021003) and PPM1D (GenBank Acc. No. NM—003620).

[0177] The term “transcription factors” is used herein to describe any protein or peptide involved in the transcription of protein-coding genes. Transcription factors may include Sp1, Sp2 (GenBank Acc. No. NM 003110), Sp3 (GenBank Acc. No. AY070137), Sp4 (GenBank Acc. No. NM—003112) NFYB (GenBank Acc. No. NM—006166), Hap2 (GenBank Acc. No. M59079), GATA-1 (GenBank Acc. No. NM—002049), GATA-2 (GenBank Acc. No. NM—002050), GATA-3 (GenBank Acc. No. X55122), GATA-4 (GenBank Acc. No. L34357), GATA-5, GATA-6 (GenBank Acc. No. NM—005257), FOG2 (NM—012082), Eryfl (GenBank Acc. No. X17254), TRPS1 (GenBank Acc. No. NM—014112), NF-E2 (GenBank Acc. No. NM—006163), NF-E3, NF-E4, TFCP2 (GenBank Acc. No. NM—005653), Oct-i (GenBank Acc. No. X13403), homeobox proteins such as HOXB2 (GenBank Acc. No. NM—002145), HOX2H (GenBank Acc. No. X16665), hairless homolog (GenBank Acc. No. NM—005144), mothers against decapentaplegic proteins such as MADH1 (GenBank Acc. No. NM—005900), MADH2 (GenBank Acc. No. NM—005901), MADH3 (GenBank Acc. No. NM—005902), MADH4 (GenBank Acc. No. NM—005359), MADH5 (GenBank Acc. No. AF009678), MADH6 (GenBank Acc. No. NM—005585), MADH7 (GenBank Acc. No. NM—005904), MADH9 (GenBank Acc. No. NM—005905), and signal transducer and activator of transcription proteins such as STATI (GenBank Acc. No. XM—010893), STAT2 (GenBank Acc. No. NM—005419), STAT3 (GenBank Acc. No. AJ012463), STAT4 (GenBank Acc. No. NM—003151), STAT5 (GenBank Acc. No. L41142), and STAT6 (GenBank Acc. No. NM—003153).

[0178] In yet another embodiment of the invention, the therapeutic molecule is a non-human or non-mammalian protein. For example, HIV gp120, HIV Tat, surface proteins of other viruses such as hepatitis, herpes, influenza, adenovirus and RSV, other HIV components, parasitic surface proteins such as malarial antigens, and bacterial surface proteins are preferred. These non-human proteins may be used, for example, as antigens, or because they have useful activities. For example, the therapeutic molecule may be streptokinase, staphylokinase, urokinase, or other proteins with useful enzymatic activities.

[0179] In an alternative embodiment, the therapeutic molecule is a ligand-binding protein with biological activity. Such ligand-binding proteins may, for example, (1) block receptor-ligand interactions at the cell surface; or (2) neutralize the biological activity of a molecule in the fluid phase of the blood, thereby preventing it from reaching its cellular target. In some embodiments, the modified transferrin fusion proteins include a modified transferrin molecule fused to a ligand-binding domain of a receptor selected from the group consisting of, but not limited to, a low density lipoprotein (LDL) receptor, an acetylated LDL receptor, a tumor necrosis factor α receptor, a transforming growth factor β receptor, a cytokine receptor, an immunoglobulin Fe receptor, a hormone receptor, a glucose receptor, a glycolipid receptor, and a glycosaminoglycan receptor. In other embodiments, ligand-binding proteins include CD2 (M14362), CD3G (NM—000073), CD3D (NM—000732), CD3E (NM—000733), CD3Z (J04132), CD28 (NM—006139), CD4 (GenBank Acc. No. NM—000616), CD1A (GenBank Acc. No. M28825), CD1B (GenBank Acc. No. NM—001764), CD1C (GenBank Acc. No. NM—001765), CD1D (GenBank Acc. No. NM—001766), CD80 (GenBank Acc. No. NM—005191), GNB3 (GenBank Acc. No. AF501884), CTLA-4 (GenBank Acc. No. NM—005214), intercellular adhesion molecules such as ICAM-1 (NM—000201), ICAM-2 (NM—000873), and ICAM-3 (NM—002162), tumor necrosis factor receptors such as TNFRSF1A (GenBank Acc. No. X55313), TNFR1SFB (GenBank Acc. No. NM—001066), TNFRSF9 (GenBank Acc. No. NM—001561), TNFRSF10B (GenBank Acc. No. NM—003842), TNFRSF11B (GenBank Acc. No. NM—002546), and TNFRSF13B (GenBank Acc. No. NM—006573), and interleukin receptors such as IL2RA (GenBank Acc. No. NM—000417), IL2RG (GenBank Acc. No. NM—000206), IL4R (GenBank Acc. No. AF421855), IL7R (GenBank Acc. No. NM—002185), IL9R (GenBank Acc. No. XM—015989), and IL13R (GenBank Acc. No. X95302). Preferably, the Tf-ligand-binding protein fusion of the present invention displays the biological activity of the ligand-binding protein.

[0180] The term “cancer-associated proteins” is used herein to describe proteins or polypeptides whose expression is associated with cancer or the maintenance of controlled cell growth, such as proteins encoded by tumor suppressor genes or oncogenes. Cancer-associated proteins may be p16 (GenBank Acc. No. AH005371), p53 (GenBank Acc. No. NM—000546), p63 (GenBank Acc. No. NM 003722), p73 (GenBank Acc. No. NM—005427), BRCA1 (GenBank Acc. No. U14680), BRCA2 (GenBank Acc. No. NM—000059), CTBP interacting protein (GenBank Acc. No. U72066), DMBT1 (GenBank Acc. No. NM—004406), HRAS (GenBank Acc. No. NM—005343), NCYM (GenBank Acc. No. NM—006316), FGR (GenBank Acc. No. NM—005248), myb (GenBank Acc. No. AF104863), raf1 (GenBank Acc. No. NM—002880), erbB2 (GenBank Acc. No. NM—004448), VAV (GenBank Acc. No. X16316), c-fos (V GenBank Acc. No. 01512), c-fes (GenBank Acc. No. X52192), cjun (GenBank Acc. No. NM—002228), MAS1 (GenBank Acc. No. M13150), pim-1 (GenBank Acc. No. M16750), TIF1 (GenBank Acc. No. NM—003852), c-fins (GenBank Acc. No. X03663), EGFR (GenBank Acc. No. NM—005228), erbA (GenBank Acc. No. X04707), c-src tyrosine kinase (GenBank Acc. No. XM—044659), c-ab1 (GenBank Acc. No. M14752), N-ras (GenBank Acc. No. X02751), K-ras (GenBank Acc. No. M54968), jun-B (GenBank Acc. No. M29039), c-myc (GenBank Acc. No. AH001511), RB1 (GenBank Acc. No. M28419), DCC (GenBank Acc. No. X76132), APC (GenBank Acc. No. NM—000038), NF1 (GenBank Acc. No. M89914), NF2 (GenBank Acc. No. Y18000), and bcl-2 (GenBank Acc. No. M13994).

[0181] “Fusogenic inhibitor peptides” is used herein to describe peptides that show antiviral activity, anti-membrane fusion capability, and/or an ability to modulate intracellular processes, for instance, those involving coiled-coil peptide structures. Antiviral activity includes, but is not limited to, the inhibition of HIV-1, HIV-2, RSV, SIV, EBV. Measles virus, influenza virus, or CMV transmission to uninfected cells. Additionally, the antifusogenic capability, antiviral activity or intracellular modulatory activity of the peptides merely requires the presence of the peptides and specifically does not require the stimulation of a host immune response directed against such peptides. Antifusogenic refers to a peptide's ability to inhibit or reduce the level of membrane fusion events between two or more moieties relative to the level of membrane fusion which occurs between said moieties in the absence of the peptide. The moieties may be, for example, cell membranes or viral structures, such as viral envelopes or pili. The term “antiviral peptide”, as used herein, refers to the peptide's ability to inhibit viral infection of cells or some viral activity required for productive viral infection and/or viral pathogenesis, via, for example, cell-cell fusion or free virus infection. Such infection may involve membrane fusion, as occurs in the case of enveloped viruses, or some other fusion event involving a viral structure and a cellular structure. Fusogenic inhibitor peptides and antiviral peptides often have amino acid sequences that are derived from greater than one viral protein (e.g., an HIV-1, HIV-2, RSV, and SIV-derived polypeptide).

[0182] Examples of fusogenic inhibitor peptides and antiviral peptides can be found in WO 94/2820, WO 96/19495, WO 96/40191, WO 01/64013 and U.S. Pat. Nos. 6,333,395, 6,258,782, 6,228,983, 6,133,418, 6,093,794, 6,068,973, 6,060,065, 6,054,265, 6,020,459, 6,017,536, 6,013,263, 5,464,933, 5,346,989, 5,603,933, 5,656,480, 5,759,517, 6,245,737; 6,326,004, and 6,348,568; all of which are herein incorporated by reference. In a preferred embodiment, antifusogenic peptides are selected from the group consisting of

2HIV T-20(FWNWLSAWKDLELLEQENKEQQNQSEEILSHILSTY,SEQ ID NO: 4),HIV T-1249, RSV T786(VYPSDEYDASISQVNEEINQALAYIRKADELLENV,SEQ ID NO: 5),RSV T1584(AVSKVLHLEGEVNKIKSALLSTNKAVVSLSNGVSVLTSKVLDLKNYIDKQL andSEQ ID NO: 6)RSV T112(VFPSDEFDASISQVNEKINQSLAFIRESDELLHNV,.SEQ ID NO: 7)

[0183] Examples of other types of peptides, include fragments of therapeutic proteins as described herein, in particular, fragments of human proteins that retain at least one activity of the parent molecule. Peptides that may be used to produce modified TF fusion proteins of the invention also include mimetic peptides and peptides that exhibit a biological activity of a therapeutic protein but differ in sequence or three-dimensional structure from a full-length therapeutic protein. As a non-limited example, peptides include erythropoeitin mimetic peptides disclosed by Johnson et al. (2000) Nephrol. Dial. Transplant 15(9): 1274-7, Kuai et al. (2000) J. Pept. Res. 56(2):59-62, Barbone et al. (1999) Nephrol. Dial. Transplant. 14 Supp 2:80-4, Middleton et al. (1999) J. Biol. Chem. 274(20):14163-9, Johnson et al. (1998) Biochemistry 37(11):3699-710, Johnson et al. (1997) Chem. Biol. 12:939-50, Wrighton et al. (1997) Nat. Biotechnol. 15(12):1261-5, Livnah et al. (1996) Science 273:464-71, and Wrighton et al., (1996) Science 273:458-64.

[0184] Therapeutic molecules also include allergenic proteins and digested fragments thereof. These include pollen allergens from ragweed, rye, June grass, orchard grass, sweet vernal grass, red top grass, timothy grass, yellow dock, wheat, corn, sagebrush, blue grass, California annual grass, pigweed, Bermuda grass, Russian thistle, mountain cedar, oak, box elder, sycamore, maple, elm, etc., dust and mites, bee venom, food allergens, animal dander, and other insect venoms.

[0185] Other therapeutic molecules include microbial vaccines which include viral, bacterial and protozoal vaccines and their various components such as surface antigens. These include vaccines which contain glycoproteins, proteins or peptides derived from these proteins. Such vaccines are prepared from Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumonia, Neisseria meningitidis, Neisseria gonorrhoeae, Salmonellae species, Shigellae species, Escherichia coli, Klebsiellae species, Proteus species, Vibrio cholera, Campylobacter pylori, Pseudomonas aeraginosa, Haemophilus influenzae, Bordetella pertussis, Mycobacterium tuberculosis, Legionella pneumophila, Treponema pallidum, chlamydia, tetanus toxoid, diphtheria toxoid, influenza viruses, adenoviruses, paramyxoviruses (mumps, measles), rubella viruses, polio viruses, hepatitis viruses, herpes viruses, rabies virus, HIV-1, HIV-2, RSV and papilloma viruses.

[0186] Preferred fusion molecules may contain anti-HIV viral peptides, anti-RSV peptides, human growth hormone, α and/or β interferons, erythropoietin (EPO), EPO like peptides, granulocyte-colony stimulating factor (GCSF), granulocyte-macrophage colony-stimulating factor (GMCSF), insulin, insulin-like growth factor (IGF), thrombopocitin, peptides corresponding to the CDR of an antibody, Islet Neogenesis Associated Protein (INGAP), calcitonin, angiostatin, endostatin, interleukin-2, growth hormone releasing factor, human parathyroid hormone, anti-tumor necrosis factor (TNF) peptides, interleukin-1 (IL-1) receptor and/or single chain antibodies.

[0187] Fusion proteins of the invention may also be prepared to include peptides or polypeptides derived from peptide libraries to screen for molecules with new or novel functions. Such peptide libraries may include those commercially or publicly available, e.g., American Peptide Co. Inc., Cell Sciences Inc., Invitrogen Corporation, Phoenix Pharmaceuticals Inc., United States Biological, as well as those produced by available technologies, e.g., bacteriophage and bacterial display libraries made using standard procedures.

[0188] In yet other embodiments of the invention, Tf fusion proteins may be prepared by using therapeutic protein moieties as known in the art and exemplified by the peptides and proteins currently approved by the Food and Drug Administitration at (www.fda.gov/cber/efoi/approve.htm) as well as PCT Patent Publication Nos. WO 01/79258, WO 01/77137, WO 01/79442, WO 01/79443, WO 01/79444 and WO 01/79480, all of which are herein incorpoated by reference in their entirety.

[0189] Table 1 (adapted from PCT International Publication No. WO 01/79444) provides a non-exhaustive list of therapeutic proteins that correspond to a therapeutic protein portion of a modified transferrin fusion protein of the invention. The “Therapeutic Protein X” column discloses therapeutic protein molecules followed by parentheses containing scientific and brand names that comprise or alternatively consist of that therapeutic protein molecule or a fragment or variant thereof. “Therapeutic protein X” as used herein may refer either to an individual therapeutic protein molecule (as defined by the amino acid sequence obtainable from the CAS and Genbank accession numbers), or to the entire group of therapeutic proteins associated with a given therapeutic protein molecule disclosed in this column. The ‘Exemplary Identifier’ column provides Chemical Abstracts Services (CAS) Registry Numbers (published by the American Chemical Society) and/or Genbank Accession Numbers (e.g., Locus ID, NP-XXXXX (Reference Sequence Protein), and XP-XXXXX (Model Protein) identifiers available through the national, Center for Biotechnology Information (NCBI) webpage at www.ncbi.nlm.nih.gov) that correspond to entries in the CAS Registry or Genbank database which contain an amino acid sequence of the protein molecule or of a fragment or variant of the therapeutic protein molecule. In addition GenSeq Accession numbers and/or journal publication citations are given to identify the exemplary amino acid sequence for some polypeptides.

[0190] The summary pages associated with each of these CAS and Genbank and GenSeq Accession Numbers as well as the cited journal publications are available (e.g., PubMed ID number (PMID)) and are herein incorporated by reference in their entirety. The PCT/Patent Reference column provides U.S. Patent numbers, or PCT International Publication Numbers corresponding to patents and/or published patent-applications that describe the therapeutic protein molecule all of which are herein incorporated by reference in their entirety. The Biological Activity column describes biological activities associated with the therapeutic protein molecule. The Exemplary Activity Assay column provides references that describe assays which may be used to test the therapeutic and/or biological activity of a therapeutic protein or a transferrin fusion protein of the invention comprising a therapeutic protein X portion. These references are also herein incorporated by reference in their entirety. “The Preferred Indication Y” column describes disease, disorders, and/or conditions that may be treated, prevented, diagnosed, or ameliorated by therapeutic protein X or a transferrin fusion protein of the invention comprising a therapeutic protein X portion.

3TABLE 1TherapeuticExemplaryPCT/PatentPreferred IndicationProtein XIdentifierReferenceBiological ActivityExemplary Activity AssayYBMP-1GeneSeqWO8800205BMP1 belongs to the transforming growthBMP-1 activity can be determinedInduction of Cartilage, TissueAcessionfactor-beta (TGFB) superfamily. Boneusing the following assays knownand Bone Growth, andP80618morphogenic proteins induce cartilage andin the art: Nat Genet. 2001Diabetesbone formation, play important role inJan.; 27(1):84-8; Eur J Biochemnephrogesis, and play an important role in1996 Apr. 1; 237(1):295-302; J Biolthe development of many organs, includingChem, Vol. 274, Issue 16, 10897-lung, heart, teeth, gut, skin, and10902, Apr. 16, 1999; and Hogan,particularly the kidney.B. L. M. (1996) Genes Dev.10, 1580-1594.BMP-2GeneSeqWO8800205BMP-2 belongs to the transforming growthBMP-2 activity can be determinedInduction of Cartilage, TissueAccessionfactor-beta (TGFB) superfamily. Boneusing the following assays known inand Bone Growth, andP80619morphogenic protein induces bonethe art: Nat Genet. 2001 Jan.;Diabetesformation.27(1):84-8; Eur J Biochem 1996Apr. 1; 237(1):295-302;J Biol Chem, Vol. 274, Issue 16,10897-10902, Apr. 16, 1999;and Hogan, B. L. M.(1996) Genes Dev. 10, 1580-1594.BMP-2BGeneSeqUS5631142BMP-2b belongs to the transforming growthBMP-2b activity can be determinedInduction of Cartilage, TissueAccessionfactor-beta (TGFB) superfamily. Boneusing the following assays known inand Bone Growth, andW24850morphogenic protein induces bonethe art: Nat Genet. 2001 Jan.;Diabetesformation.27(1):84-8; Eur J Biochem 1996Apr. 1; 237(1):295-302;I Biol Cbcre, Vol. 274, Issue 16,10897-10902, Apr. 16, 1999;and Hogan, B. L. M. (1996) GenesDev. 10, 1580-1594.BMP-4GeneSeqWO0020591BMP-4 belongs to the transforming growthBMP-4 activity can be determinedInduction of Cartilage, TissueAccessionfactor-beta (TGFB) superfamily. Boneusing the following assays known inand Bone Growth, andB02796morphogenic protein induces bonethe art: Nat Genet. 2001 Jan.;Diabetesformation.27(1):84-8; Eur J Biochem 1996Apr. 1; 237(1):295-302; J BiolChem, Vol. 274, Issue 16, 10897-10902, Apr. 16, 1999; and Hogan,B. L. M. (1996) Genes Dev.10, 1580-1594.BMP-5GeneSeqWO0020591BMP-5 belongs to the transforming growthBMP-5 activity can be determinedInduction of Cartilage, TissueAccessionfactor-beta (TGFB) superfamily. Boneusing the following assays known inand Bone Growth, andB02797morphogenic protein induces bonethe art: Nat Genet. 2001 Jan.;Diabetesformation.27(1):84-8; Eur J Biochem 1996Apr. 1; 237(1):295-302; J BiolChem, Vol. 274, Issue 16, 10897-10902, Apr. 16, 1999; and Hogan,B. L. M. (1996) Genes Dev.10, 1580-1594.BMP-6GeneSeqUS5187076BMP-6 belongs to the transforming growthBMP-6 activity can be determinedInduction of Cartilage, TissueAccessionfactor-beta (TGFB) superfamily. Boneusing the following assays known inand Bone Growth, andR32904morphogenic protein induces bonethe art: Nat Genet. 2001 Jan.;Diabetesformation.27(1):84-8; Eur J Biochem 1996Apr. 1; 237(1):295-302; J BiolChem, Vol. 274, Issue 16, 10897-10902, Apr. 16, 1999; and Hogan,B. L. M. (1996) Genes Dev.10, 1580-1594.Osteo-GeneSeqWO973462OP-1 belongs to the transforming growthOP-1 activity can be determinedInduction of Cartilage, TissuegenicAccessionfactor-beta (TGFB) superfamily. Boneusing the following assays known inand Bone Growth, andProtein-1;W34783morphogenic protein induces bonethe art: Nat Genet. 2001 Jan.,DiabetesOP-1;formation.27(1):84-8; Eur J Biochem 1996BMP-7Apr. 1; 237(1):295-302; J BiolChem, Vol. 274, Issue 16, 10897-10902, Apr. 16, 1999; and Hogan,B. L. M. (1996) Genes Dev. 10,1580-1594.Osteo-GeneSeqWO9406399OP-2 belongs to the transforming growthOP-2 activity can be determinedInduction of Cartilage, TissuegenicAccessionfactor-beta (TGFB) superfamily. Boneusing the following assays known inand Bone Growth, andProtein-2R57973morphogenic protein induces bonethe art: Nat Genet. 2001 Jan.;Diabetesformation.27(1):84-8; Eur J Biochem 1996Apr. 1; 237(1):295-302; J BiolChem, Vol. 274, Issue 16, 10897-10902, Apr. 16, 1999; and Hogan,B. L. M. (1996) Genes Dev. 10,1580-1594.GDP-1GeneSeqWO9406449Members of the TGF-betaThe effect of GDF-1 on signalingDevelopmental disorders,Accessionfamily of proteinscan be assayed by treating PrimaryInduction of Cartilage, TissueR60961initiate cell signaling by binding toBAECs transferred with a constructand Bone Growth, andheteromeric receptor complexes of type Icalled p3TP-Lux, containing a TGF-Diabetes(TbetaRI) and type II (TbetaRII)beta responsive promoter fused to aserine/threonine kinase receptors (reviewedreporter gene, and measuringby Massague, J. et al. (1994) Trends Cellluciferase gene expression (Wrana etBiol. 4:172 178; Miyazono, K. et al. (1994)al, 1994, Nature 370: 341-347).Adv. Immunol. 55:181-220). Activation ofthis heteromeric receptor complex occurswhen TGF-beta binds to TbetaRII, whichthen recruits and phosphorylates TbetaRI.Activated TbetaRI then propagates thesignal to downstream targets (Chen, F. andWeinberg, R. A. (1995) PNA892:1565-1569;Wrana, J. L. et al. (1994) Nature 370:341347).BMP-9GeneSeqWO9533830BMP-9 belongs to the transforming growthBMP-9 activity can be determinedInduction of Cartilage, TissueAccessionfactor-beta (TGFB) superfamily. Boneusing the following assays known inand Bone Growth, andR86903morphogenic protein induces bonethe art: Nat Genet. 2001 Jan.;Diabetesformation.27(1):84-8; Eur J Biochem 1996Apr. 1; 237(1):295-302; J BiolChem, Vol. 274, Issue 16, 10897-10902, Apr. 16, 1999; and Hogan,B. L. M. (1996) Genes Dev. 10,1580-1594.BMP-10GeneSeqWO9426893BMP-10 belongs to the transforming growthBMP-10 activity can be determinedInduction of Cartilage, TissueAccessionfactor-beta (TGFB) superfamily. Boneusing the following assays known inand Bone Growth, andR66202morphogenic protein induces bonethe art: Nat Genet. 2001 Jan.;Diabetesformation.27(1):84-8; Eur J Biochem 1996Apr. 1; 237(1):295-302; J BiolChem, Vol. 274, Issue 16, 10897-10902, Apr. 16, 1999; and Hogan,B. L. M (1996) Genes Dev.10, 1580-1594.BMP-12GeneSeqWO9516035BMP-12 belongs to the transforming growthBMP-12 activity can be determinedInduction of Cartilage, TissueAccessionfactor-beta (TGFB) superfamily. Boneusing the following assays known inand Bone Growth, andR78734morphogenic protein induces bonethe art: Nat Genet. 2001 Jan.;Diabetesformation.27(1):84-8; Eur J Biochem 1996Apr. 1; 237(1):295-302; J BiolChem, Vol. 274, Issue 16, 10897-10902, Apr. 16, 1999; and Hogan,B. L. M. (1996) Genes Dev.10, 1580-1594.BMP-15GeneSeqW09636710BMP-15 belongs to the transforming growthBMP-15 activity can be determinedInduction of Cartilage, TissueAccessionfactor-beta (TGFB) superfamily. Boneusing the following assays known inand Bone Growth, andW11261morphogenic protein induces bonethe art: Nat Genet. 2001 Jan.;Diabetesformation.27(1):84-8; Eur J Biochem 1996Apr. 1; 237(1):295-302; J BiolChem, Vol. 274, Issue 16, 10897-10902, Apr. 16, 1999; and Hogan,B. L. M. (1996) Genes Dev.10, 1580-1594.BMP-17GeneSeqWO9929718BMP-17 belongs to the transforming growthBMP-17 activity can be determinedInduction of Cartilage, TissueAccessionfactor-beta (TGFB) superfamily. Boneusing the following assays known inand Bone Growth, andY17870morphogenic protein induces bonethe art: Nat Genet. 2001 Jan.;Diabetesformation.27(1):84-8; Eur J Biochem 1996Apr. 1; 237(1):295-302; J BiolChem, Vol. 274, Issue 16, 10897-10902, Apr. 16, 1999; and Hogan,B. L. M. (1996) Genes Dev.10, 1580-1594.BMP-18GeneSeqWO9929718BMP-18 belongs to the transforming growthBMP-18 activity can be determinedInduction of Cartilage, TissueAccessionfactor-beta (TGFB) superfamily. Boneusing the following assays known inand Bone Growth, andY17871morphogenic protein induces bonethe art: Nat Genet. 2001 Jan.;Diabetesformation.27(1):84-8; Eur J Biochem 1996Apr. 1; 237(1):295-302; J BiolChem, Vol. 274, Issue 16, 10897-10902, Apr. 16, 1999; and Hogan,B. L. M. (1996) Genes Dev.10, 1580-1594.InhibinGeneSeqWO0020591The inhibin beta A subunit joins the alphaTumor suppressor activity of inhibinTumor suppression.alphaAccessionsubunit to form a pituitary FSH secretioncan be determined using assaysB02806inhibitor. Inhibin has been shown toknown in the art: Matzuk et al.,regulategonadal stromal cell proliferationNature 1992 Nov. 26: 360negatively and to have tumour-suppressor(6402); 313-9.activity. In addition, serum levels of inhibinhave been shown to reflect the size ofgranulosa-cell tumors and can therefore beused as a marker for primary as well asrecurrent disease.InhibinGeneSeqWO0020591The inhibin beta A subunit joins the alphaTumor suppressor activity of inhibinTumor suppression.betaAccessionsubunit to form a pituitary FSH secretioncan be determined using assaysH02808inhibitor. Inhibin has been shown toknown in the art: Matzuk et al.,regulategonadal stromal cell proliferationNature 1992 Nov. 26: 360negatively and to have tumour-suppressor(6402); 313-9.activity. In addition, serum levels of inhibinhave been shown to reflect the size ofgranulosa-cell tumors and can therefore beused as a marker for primary as well asrecurrent disease.CerebusGeneSeqWO9849296Cerebus is believed to be involved in theBMP activity, in the presence of theBMP Antagonist useful forProteinAccessioninhibition of BMP activityantagonist Cerebus, can beOsteosarcoma, abnormal boneW86032determined using the followinggrowth.assays known in the art: Nat Genet.2001 Jan.; 27(1):84-8; Eur JBiochem 1996 Apr. 1; 237(1):295-302; J Biol Chem, Vol. 274, Issue16, 10897-10902, Apr. 16, 1999;and Hogan, B. L. M. (1996) GenesDev. 10, 1580-1594.SolubleGeneSeqWO9614579Soluble BMP receptor kinase protein-3 isBMP activity, in the presence of theBMP Antagonist useful forBMPAccessioninvolved in the binding of BMPs. Solublesoluble antagonist BMP receptorOsteosarcoma, abnormal boneReceptorR95227BMP receptor kinase protein-3 is useful askinase protein-3, can be determinedgrowth.Kinasean antagonist for the inhibition of BMPusing the following assays known inProtein-3activity.the art: Nat Genet. 2001 Jan.;27(1):84-8; Eur J Biochem 1996Apr. 1; 237(1):295-302; J BiolChem, Vol. 274, Issue 16, 10897-10902, Apr. 16, 1999; and Hogan,B. L. M. (1996) Genes Dev.10, 1580-1594.BMPGeneSeqWO9741250BMPs belong to the transforming growthBMP activity, in the presence of theBone formation orPro-Accessionfactor-beta (TGFB) superfamily. BoneFurin, can be determined using theRegeneration AbnormalitiescessingW36099morphogenic protein induces bonefollowing assays known in the art:Enzymeformation.Nat Genet. 2001 Jan.; 27(1):84-8;FurinEur J Biochem 1996 Apr. 1;237(1):295-302; J Biol Chem, Vol.274, Issue 16, 10897-10902, Apr.16, 1999; and Hogan, B. L. M.(1996) Genes Dev. 10, 1580-1594.TGF-GeneSeqWO9216228Members of the TGF-betaThe effect of TGF betas on signalingUseful for treating cancer andbeta 1Accessionfamily of proteinscan be assayed by treating Primaryto promote wound healing.R29657initiate cell signaling by binding toBAECs transfected with a constructheteromeric receptor complexes of type Icalled p3TP-Lux, containing a TGF-(TbetaRI) and type II (TbetaRII)beta responsive promoter fused to aserine/threonine kinase receptors (reviewedreporter gene, and measuringby Massague, J. et al. (1994) Trends Cellluciferase gene expression (Wrana etBiol. 4:172 178; Miyazono, K. et al. (1994)al., 1994, Nature 370:341-347).Adv. Immunol. 55:181-220). Activation ofthis heteromeric receptor complex occurswhen TGF-beta. binds to TbetaRII, whichthen recruits and phosphorylates TbetaRI.Activated TbetaRI then propagates thesignal to downstream targets (Chen, F. andWeinberg. R. A. (1995) PNA892:1565-1569; Wrana, J. L. et al.(1994) Nature 370:341.TGF-GeneSeqEP542679Members of the TGF-betaThe effect of TGF betas on signalingUseful for treating cancer andbeta 2Accessionfamily of proteinscan be assayed by treating Primaryto promote wound healing.R39659initiate cell signaling by binding toBAECs transfected with a constructheteromeric receptor complexes of type Icalled p3TP-Lux, containing a TGF-(TbetaRI) and type II (TbetaRII)beta responsive promoter fused to aserine/threonine kinase receptors (reviewedreporter gene, and measuringby Massague, J. et al. (1994) Trends Cellluciferase gene expression (Wrana etBiol. 4:172 178; Miyazono, K. et al. (1994)al., 1994, Nature 370:341-347).Adv. Immunol. 55:181-220). Activation ofthis heteromeric receptor complex occurswhen TGF-beta. binds to TbetaRII, whichthen recruits and phosphorylates TbetaRI.Activated TbetaRI then propagates thesignal to downstream targets (Chen, F. andWeinberg. R. A. (1995) PNA892:1565-1569; Wrana, J. L. et al.(1994) Nature 370:341.ZTGF-GeneSeqWO0015798Members of the TGF-betaThe effect of TGF betas on signalingUseful for treating cancer andbeta 9Accessionfamily of proteinscan be assayed by treating Primaryto promote wound healing.Y70654initiate cell signaling by binding toBAECs transfected with a constructheteromeric receptor complexes of type Icalled p3TP-Lux, containing a TGF-(TbetaRI) and type II (TbetaRII)beta responsive promoter fused to aserine/threonine kinase receptors (reviewedreporter gene, and measuringby Massague, J. et al. (1994) Trends Cellluciferase gene expression (Wrana etBiol. 4:172 178; Miyazono, K. et al. (1994)al., 1994, Nature 370:341-347).Adv. Immunol. 55:181-220). Activation ofthis heteromeric receptor complex occurswhen TGF-beta. binds to TbetaRII, whichthen recruits and phosphorylates TbetaRI.Activated TbetaRI then propagates thesignal to downstream targets (Chen, F. andWeinberg. R. A. (1995) PNA892:1565-1569; Wrana, J. L. et al.(1994) Nature 370:341.Anti-TGFGB2305921Members of the TGF-betaThe effect of TGF betas on signalingUseful for control of fibrosis,betafamily of proteinsin the presence of an anti-TGF betaimmune, and inflammatoryfamilyinitiate cell signaling by binding toantibody, can be assayed by treatingdisease.anti-heteromeric receptor complexes of type IPrimary BAECs transfected with abodies(TbetaRI) and type II (TbetaRII)construct called p3TP-Lux,serine/threonine kinase receptors (reviewedcontaining a TGF-beta responsiveby Massague, J. et al. (1994) Trends Cellpromoter fused to a reporterBiol. 4:172 178; Miyazono, K. et al. (1994)gene, and measuring luciferase geneAdv. Immunol. 55:181-220). Activation ofexpression (Wrana et al., 1994,this heteromeric receptor complex occursNature 370:341-347).when TGF-beta. binds to TbetaRII, whichthen recruits and phosphorylates TbetaRI.Activated TbetaRI then propagates thesignal to downstream targets (Chen, F. andWeinberg. R. A. (1995) PNA892:1565-1569; Wrana, J. L. et al.(1994) Nature 370:341.LatentGeneSeqWO0012551Members of the TGF-betaThe effect of TGF betas on signalingUseful for inhibiting tissue orTGF betaAccessionfamily of proteinsin the presence of a TGF betatumor growth.bindingY70552initiate cell signaling by binding tobinding protein, can be assayed byproteinheteromeric receptor complexes of type Itreating Primary BAECs transfectedII(TbetaRI) and type II (TbetaRII)with a construct called p3TP-Lux,serine/threonine kinase receptors (reviewedcontaining a TGF-beta responsiveby Massague, J. et al. (1994) Trends Cellpromoter fused to a reporter gene,Biol. 4:172 178; Miyazono, K. et al. (1994)and measuring luciferase geneAdv. Immunol 55:181-220). Activation ofexpression (Wrana et al., 1994,this heteromeric receptor complex occursNature 370:341-347).when TGF-beta. binds to TbetaRII, whichthen recruits and phosphorylates TbetaRI.Activated TbetaRI then propagates thesignal to downstream targets (Chen, F. andWeinberg. R. A. (1995) PNA892:1565-1569; Wrana, J. L. et al.(1994) Nature 370:341.MP52GeneSeqWO9741250Members of the TGF-betaThe effect of TGF betas on signalingBone formation orAccessionfamily of proteinscan be assayed by treating PrimaryRegeneration AbnormalitiesW36100initiate cell signaling by binding toBAECs transfected with a constructheteromeric receptor complexes of type Icalled p3TP-Lux, containing a TGF-(TbetaRI) and type II (TbetaRII)beta responsive promoter fused to aserine/threonine kinase receptors (reviewedreporter gene, and measuringby Massague, J. et al. (1994) Trends Cellluciferase gene expression (Wrana etBiol. 4:172 178; Miyazono, K. et al. (1994)al., 1994, Nature 370:341-347).Adv. Immunol. 55:181-220). Activation ofthis heteromeric receptor complex occurswhen TGF-beta. binds to TbetaRII, whichthen recruits and phosphorylates TbetaRI.Activated TbetaRI then propagates thesignal to downstream targets (Chen, F. andWeinberg. R. A. (1995) PNA892:1565-1569; Wrana, J. L. et al.(1994) Nature 370:341.b57GeneSeqWO9837195BMPs are involved in the induction of boneBMP activity, in the presence of b57BMP Antagonist useful forProteinAccessionformation. Specific antagonists are useful isprotein, can be determined using theOsteosarcoma, abnormal boneW69293preventing this activity from occurring.following assays known in the art:growth.Nat Genet. 2001 Jan.; 27(1):84-8;Eur J Biochem 1996 Apr. 1; 237(1):295-302; J Biol Chem, Vol. 274,Issue 16, 1089-10902, Apr. 16,1999; and Hogan, B. L. M. (1996)Genes Deve. 10, 1580-1594.ResistinGeneSeqWO0064920This gene belongs to the family defined byAbility of resistin to influence typeType II diabetes andAccessionmouse FIZZ1 and FIZZ3/Resistin genes. TheII diabetes can be determined usingSyndrome X.W69293characteristic feature of this family is the C-assays known in the art: Pontoglioterminal stretch of 10 cys residues withet al., J Clin Invest 1998 May 15;identical spacing. The mouse homolog of101(10):2215-22.this protein is secreted by adipocytes, may bethe hormone potantially linking obesity totype II diabetes.Galectin-4GeneSeqWO9703190Galectins are a family of carbohydrate-Ability of Galectin-4 polypeptidesLactose intolerance.Accessionbinding proteins characterized by an affinityto bind lactose can be determinedW11841for beta-galactoside containingusing assays known in the art:glycoconjugates.Wada, et al., J Biol Chem 1997Feb. 28; 272(9):6078-86.APM-I;GeneSeqW00026363ACPR30 gene is exclusively expressed inAbility of ACRP30 polypeptides toObesity, Metabolic disorders,ACRP-30;Accessionadipose tissue. ACRP30 is thought toinfluence obesity and fat oxidationLipid Metabolism; HormoneFamoxinY71035increase fatty acid oxidation by musclecan be determined using assaysSecretion.tissue.known in the art: Fruebis et al.,Proc Nat'l Acad Sci USA 2001Feb. l3; 98(4):2005-10.ACRP-30GeneSeqWO0063376ACPR30 gene is exclusively expressed inAbility of ACRP30 homologueObesity, Metabolic disorders,Homologue;Accessionadipose tissue. ACRP30 is thought topolypeptides to influence obesityLipid Metabolism; HormoneComplementB30234increase fatty acid oxidation by muscleand fat oxidation can be determinedSecretion.Componenttissue.using assays known in the art:Clq CFruebis et al., Proc Nat'l Acad SciUSA 2001 Feb. 13; 98(4):2005-10.Calpain-10aGeneSeqWO0023603Calpain is believed toAbility of Calpain-10 to influenceDiabetes mellitus; RegulationAccessionplay a role in insulintype II diabetes can be determinedof Insulin secretory response;Y79567secretion and insulin activity, and thereforeusing assays known in the art:Insulin mediated glucosemay be useful in the treatment of type IIPontoglio et al., J Clin Invest 1998transport disorders.diabetes.May 15; 101(10):2215-22.Calpain-10bGeneSeqWO0023603Calpain is believed toAbility of Calpain-10 to influenceDiabetes mellitus; RegulationAccessionplay a role in insulintype II diabetes can be determinedof Insulin secretory response;Y79568secretion and insulin activity, and thereforeusing assays known in the art:Insulin mediated glucosemay be useful in the treatment of type IIPontoglio et al., J Clin Invest 1998transport disorders.diabetes.May 15; 101(10):2215-22.Calpain-10cGeneSeqWO0023603Calpain is believed toAbility of Calpain-10 to influenceDiabetes mellitus; RegulationAccessionplay a role in insulintype II diabetes can be determinedof Insulin secretory response;Y79569secretion and insulin activity, and thereforeusing assays known in the art:Insulin mediated glucosemay be useful in the treatment of type IIPontoglio et al., J Clin Invest 1998transport disorders.diabetes.May 15; 101(10):2215-22.PDGF-DGeneSeqWO0027879Vascular Endothelial Growth Factor.Proliferation assay using NR6R-Wound Healing; Atherosclermis.Accession3T3 cells (Rizzino 1988 CancerY71130Res. 48: 4266).FasLGeneSeqWO9936079Activities associated with apoptosis andActivity can be determined usingApoptosis-related disorders;Accessionimmune system functions.Apoptosis assays known in the art:Autoimmune disorders; GraftY28594Walczak et al. (1996) EMBOJ 16:v-Host disorders.5386-5397.ChondroGeneSeqW00029579Chondromodulin proteins are cartilageAbility of Chondromodulin-likeAntianglogenic agent;modulin-Accessionproteins thought to confer resistance toprotein to inhibit vascularizationOsteoblast proliferationlikeY71262anglogeneis, and thus are useful as anti-can be determined using assaysstimulator; preventsproteinangiogenic agents that may have utility inknown in the art: Hirakie et al.,vascularization of cartilagecombating cancer.J Biol Chem 1997 Dec. 19;tissue; Useful to treat cancer.272(51):32419-26.PatchedGeneSeqUS5837538Patched is a tumour-suppressorAbility of soluble Patched to bindReceptor for HedgehogAccessionreceptor for Sonic hedgehog (shh), whichto and inhibit the activities of shhcellular proliferation signalingW72969is a protein that controls developmentalcan be determined using assaysmolecule. This receptor ispatterning and growth.known in the art: Stone et al.,useful as a means ofNature 1996 Nov. 14;preventing cellular384(6605):129-34.proliferation via the shhsignaling pathway, thus usefulfor cancers.Patched-2GeneSeqWO9953058Patched is a tumour-suppressorAbility of soluble Patched to bindReceptor for HedgehogAccessionreceptor for Sonic hedgehog (shh), whichto and inhibit the activities of shhcellular proliferation signalingY43261is a protein that controls developmentalcan be determined using assaysmolecule. This receptor ispatterning and growth.known in the art: Stone et al.,useful as a means ofNature 1996 Nov. 14;preventing cellular384(6605):129-34.proliferation via the shhsignaling pathway, thus usefulfor cancers.Maspin;GeneSeqWO9405804Maspin is a member of the serpin family ofThe inhibitory effects cf MaspinTumor suppressor which isProteaseAccessionserine protease inhibitors that is thought toand other protease inhibitors can bedown-regulated in breastInhibitorR50938suppress tumor metastasis.assayed using methods known incancers. The maspin protein5the art such as a labeled proteasehas tumour suppressing andsubstrate, for example, Universalinvasion suppressing activity.Protease Substrate (casein,resorufin-labeled): RocheMolecular Biochemicals, Cat. No.1080733.EndostatinGeneSeqWO0064946Endostatin is believed to inhibit effects ofThe inhibitory effects of endostatinAnti-angiogenic activity.Accessioncapillary endothelial cell proliferation.can be assayed using assaysUseful in the prevention and/orB28399disclosed by Cao et al. (1996) J.treatment of cancers.Biol. Chem. 271 29461-29467.aFGF;GeneSeqEP298723Fibroblast Growth FactorProliferation assay using NR6R-Promotion of growth andFGF-1Accession3T3 cells (Rizzino 1988 Cancerproliferation of cells, such asP94037Res. 48: 4266); Examples 23 andepithelial cells and39 disclosed herein.keratinocytes. Antagonistsmay be useful as anti-canceragents.bFGF;GeneSeqFR2642086Fibroblast Growth FactorProliferation assay using NR6R-Promotion of growth andFGF-2Accession3T3 cells (Rizzino 1988 Cancerproliferation of cells, such asR06685Res. 48: 4266); Examples 23 andepithelial cells and39 disclosed herein.keratinocytes. Antagonistsmay be useful as anti-canceragents.FGF-3;GeneSeqWO9503831Fibroblast Growth FactorProliferation assay using NR6R-Promotion of growth andINT-2Accession3T3 cells (Rizzino 1988 Cancerproliferation of cells, such asR07824Res. 48: 4266); Examples 23 andepithelial cells and39 disclosed herein.keratinocytes. Antagonistsmay be useful as anti-canceragents.FGF-4;GeneSeqWO9503831Fibroblast Growth FactorProliferation assay using NR6R-Promotion of growth andHST-1;Accession3T3 cells (Rizzino 1988 Cancerproliferation of cells, such asHBGF-4R07825Res. 48: 4266); Examples 23 andepithelial cells and39 disclosed herein.keratinocytes. Antagonistsmay be useful as anti-canceragents.FGF-5GeneSeqWO9730155Fibroblast Growth FactorProliferation assay using NR6R-Promotion of growth andAccession3T3 cells (Rizzino 1988 Cancerproliferation of cells, such asW22600Res. 48: 4266); Examples 23 andepithelial cells and39 disclosed herein.keratinocytes. Antagonistsmay be useful as anti-canceragents.FGF-6;GeneSeqEP613946Fibroblast Growth FactorProliferation assay using NR6R-Promotion of growth andHeparinAccession3T3 cells (Rizzino 1988 Cancerproliferation of cells, such asbindingR58555Res. 48: 4266); Examples 23 andepithelial cells andsecreted39 disclosed herein.keratinocytes. Antagoniststrans-may be useful as anti-cancerformingagentsfactor-2FGF-8GeneSeqWO9524928Fibroblast Growth FactorProliferation assay using NR6R-Promotion of growth andAccession3T3 cells (Rizzino 1988 Cancerproliferation of cells, such asR80783Res. 48: 4266); Examples 23 andepithelial cells and39 disclosed herein.keratinocytes. Antagonistsmay be useful as anti-canceragents.FGF-9;GeneSeqWO9503831Fibroblast Growth FactorProliferation assay using NR6R-Promotion of growth andGilaAccession3T3 cells (Rizzino 1988 Cancerproliferation of cells, such asactivatingR70822Res. 48: 4266); Examples 23 andepithelial cells andfactor39 disclosed herein.keratinocytes. Antagonistsmay be useful as anti-canceragents.FGF-12;GeneSeqWO9635708Fibroblast Growth FactorProliferation assay using NR6R-Promotion of growth andFibroblastAccession3T3 cells (Rizzino 1988 Cancerproliferation of cells, such asgrowthW06309Res. 48: 4266); Examples 23 andepithelial cells andfactor39 disclosed herein.keratinocytes. Antagonistshomologousmay be useful as anti-cancerfactor-1agents.FGF-15GeneSeqWO9927100Fibroblast Growth FactorProliferation assay using NR6R-Promotion of growth andAccession3T3 cells (Rizzino 1988 Cancerproliferation of cells, such asY08582Res. 48: 4266); Examples 23 andepithelial cells and39 disclosed herein.keratinocytes. Antagonistsmay be useful as anti-canceragents.FGF-16GeneSeqWO9918128Fibroblast Growth FactorProliferation assay using NR6R-Promotion of growth andAccession3T3 cells (Rizzino 1988 Cancerproliferation of cells, such asY05474Res. 48: 4266); Examples 23 andepithelial cells and39 disclosed herein.keratinocytes. Antagonistsmay be useful as anti-canceragents.FGF-18GeneSeqWO9927100Fibroblast Growth FactorProliferation assay using NR6R-Promotion of growth andAccession3T3 cells (Rizzino 1988 Cancerproliferation of cells, such asY08590Res. 48: 4266); Examples 23 andepithelial cells and39 disclosed herein.keratinocytes. Antagonistsmay be useful as anti-canceragents.fit-3GeneSeqEP627487Stem Cell ProgenitorChemokine activities can bePromotion of immune cellligandAccessiondetermined using assays known ingrowth and/or differentiation.R67541the art: Methods in MolecularBiology, 2000, vol. 138:Chemokine Protocols. Edited by:A. E. I. Proudfoot, T. N. C. Wells,and C. A. Power. © Humana PressInc., Totowa, NJ.VEGF-110GeneSeqWO0013702Promotes theVEGF activity can be determinedPromotion of growth andAccessiongrowth and/or proliferationusing assays known in the art, suchproliferation of cells, such asY69417of endothelial cells.as those disclosed in Internationalvascular endothelial cells.Publication No. WO0045835, forAntagonists may be useful asexample.anti-angiogenic agents, andmay be applicable for cancer.VEGB-121GeneSeqWO0071713Promotes theVEGF activity can be determinedPromotion of growth andAccessiongrowth and/or proliferationusing assays known in the art, suchproliferation of cells, such asB50432of endothelial cells.as those disclosed in Internationalvascular endothelial cells.Publication No. WO0045835, forAntagonists may be useful asexample.anti-angiogenic agents, andmay be applicable for cancer.VEGF-138GeneSeqWO9940197Promotes theVEGF activity can be determinedPromotion of growth andAccessiongrowth and/or proliferationusing assays known in the art, suchproliferation of cells, such asY43483of endothelial cells.as those disclosed in Internationalvascular endothelial cells.Publication No. WO0045835, forAntagonists may be useful asexample.anti-angiogenic agents, andmay be applicable for cancer.VEGF-145GeneSeqWO0013702Promotes theVEGF activity can be determinedPromotion of growth andAccessiongrowth and/or proliferationusing assays known in the art, suchproliferation of cells, such asY69413of endothelial cells.as those disclosed in Internationalvascular endothelial cells.Publication No. WO0045835, forAntagonists may be useful asexample.anti-angiogenic agents, andmay be applicable for cancer.VEGF-162GeneSeqW09940197Promotes theVEGF activity can be determinedPromotion of growth andAccessiongrowth and/or proliferationusing assays known in the art, suchproliferation of cells, such asY43484of endothelial cells.as those disclosed in Internationalvascular endothelial cells.Publication No. WO0045835, forAntagonists may be useful asexample.anti-angiogenic agents, andmay be applicable for cancer.VEGF-165GeneSeqWO0013702Promotes theVEGF activity can be determinedPromotion of growth andAccessiongrowth and/or proliferation ofusing assays known in the art, suchproliferation of cells, such asY69414endothelial cells.as those disclosed in Internationalvascular endothelial cells.Publication No. WO0045835, forAntagonists may be useful asexample.anti-angiogenic agents, andmay be applicable for cancer.VEGF-182GeneSeqW09940197Promotes theVEGF activity can be determinedPromotion of growth andAccessiongrowth and/or proliferation ofusing assays known in the art, suchproliferation of cells, such asY43483endothelial cells.as those disclosed in Internationalvascular endothelial cells.Publication No. WO0045835, forAntagonists may be useful asexample.anti-angiogenic agents, andmay be applicable for cancer.VEGF-189GeneSeqWO0013702Promotes theVEGF activity can be determinedPromotion of growth andAccessiongrowth and/or proliferation ofusing assays known in the art, suchproliferation of cells, such asY69415endothelial cells.as those disclosed in Internationalvascular endothelial cells.Publication No. WO0045835, forAntagonists may be useful asexample.anti-angiogenic agents, andmay be applicable for cancer.VEGF-206GeneSeqW00013702Promotes theVEGF activity can be determinedPromotion of growth andAccessiongrowth and/or proliferation ofusing assays known in the art, suchproliferation of cells, such asY69416endothelial cells.as those disclosed in Internationalvascular endothelial cells.Publication No. WO0045835, forAntagonists may be useful asexample.anti-angiogenic agents, andmay be applicable for cancer.VEGF-DGeneSeqWO9807832Promotes theVEGF activity can be determinedPromotion of growth andAccessiongrowth and/or proliferation ofusing assays known in the art, suchproliferation of cells, such asW53240endothelial cells.as those disclosed in Internationalvascular endothelial cells.Publication No. WO0045835, forAntagonists may be useful asexample.anti-angiogenic agents, andmay be applicable for cancer.VEGF-E;GeneSeqW09947677Promotes theVEGF activity can be determinedPromotion of growth andVEGF-XAccessiongrowth and/or proliferation ofusing assays known in the art, suchproliferation of cells, such asY33679endothelial cells.as those disclosed in Internationalvascular endothelial cells.Publication No. WO0045835, forAntagonists may be useful asexample.anti-angiogenic agents, andmay be applicable for cancer.VEGFGeneSeqWO9831794Receptor for VEGF polypeptidesVEGF activity, in the presence ofVEGF Receptor. FusionReceptor;Accessionflk-1 polypeptides, can beprotein with the extracellularKDR;W69679determined using assays known indomain is useful as an anti-flk-1the art, such as those disclosed inangiogenic agent. AntagonistsInternational Publication No.may be useful in the promotionWO0045835, for example.of angiogenesis.SolubleGeneSeqUS5712380Receptor for VEGF polypeptidesVEGF activity, in the presence ofVEGF Receptor. FusionVEGFAccessionVEGF Receptor polypeptides, canprotein with the extracellularReceptorW47037be determined using assays known indomain is useful as an anti-the art, such as those disclosed inangiogenic agent. AntagonistsInternational Publication No.may be useful in the promotionWO0045835, for example.of angiogenesis.flt-1GeneSeqWO0021560Receptor for VEGF polypeptidesVEGF activity, in the presence ofVEGF Receptor. FusionAccessionflt-1 polypeptides, can beprotein with the extracellularY70751determined using assays known indomain is useful as an anti-the art, such as those disclosed inangiogenic agent. AntagonistsInternational Publication No.may be useful in the promotionWO0045835, for example.of angiogenesis.VEGF R-3;GeneSeqWO0058511Receptor for VEGF polypeptidesVEGF activity, in the presence ofVEGF Receptor. Fusionflt-4Accessionflt-4 polypeptides, can beprotein with the extracellularB29047determined using assays known indomain is useful as an anti-the art, such as those disclosed inangiogenic agent. AntagonistsInternational Publication No.may be useful in the promotionWO0045835, for example.of angiogenesis.Neuro-GeneSeqWO9929858Vascular Endothelial Growth FactorVEGF activity can be determinedPromotion of growth andpilin-1Accessionusing assays known in the art, suchproliferation of cells, such asY06319as those disclosed in Internationalvascular endothelial cells.Publication No. WO0045835, forAntagonists may be useful asexample.anti-angiogenic agents, andmay be applicable for cancer.Neuro-GeneSeqWO9929858Vascular Endothelial Growth FactorVEGF activity can be determinedPromotion of growth andpilin-2Accessionusing assays known in the art, suchproliferation of cells, such asY03618as those disclosed in Internationalvascular endothelial cells.Publication No. WO0045835, forAntagonists may be useful asexample.anti-angiogenic agents, andmay be applicable for cancer.HumanGeneSeqW09730085Troponins are contractile proteins that areAbility of soluble Troponins toAnti-angiogenesisfastAccessionthought to inhibit angiogenesis. High levelsinhibit anglogenesis can betwitchW22597may contribute to the difficulty encountereddetermined using assays known inskeletalin revascularizing the ischemic myocardiumthe art:. Proc Natl Acad Sci USAmuscleafter cardiovascular injury.1999 Mar. 16; 96(6):2645-50.troponin CHumanGeneSeqW09730085Troponins are contractile proteins that areAbility of soluble Troponins toAnti-angiogenesisfastAccessionthought to inhibit angiogenesis. High levelsinhibit anglogenesis can betwitchW18054may contribute to the difficulty encountereddetermined using assays known inskeletalin revascularizing the ischemic myocardiumthe art:. Proc Natl Acad Sci USAmuscleafter cardiovascular injury.1999 Mar. 16; 96(6):2645-50.troponin IHuman fastGeneSeqW09730085Troponins are contractile proteins that areAbility of soluble Troponins toAnti-angiogenesistwitchAccessionthought to inhibit angiogenesis. High levelsinhibit anglogenesis can beskeletalW22599may contribute to the difficulty encountereddetermined using assays known inmusclein revascularizing the ischemic myocardiumthe art:. Proc Natl Acad Sci USAtroponin Tafter cardiovascular injury.1999 Mar. 16; 96(6):2645-50.fragment.GeneSeqW09719955Troponins are contractile proteins that areAbility of soluble Troponins toAnti-angiogenesismyo-Accessionthought to inhibit angiogenesis. High levelsinhibit anglogenesis can befibrillarW18053may contribute to the difficulty encountereddetermined using assays known inproteinin revascularizing the ischemic myocardiumthe art:. Proc Natl Acad Sci USAtroponin Iafter cardiovascular injury.1999 Mar. 16; 96(6):2645-50.myo-GeneSeqW09719955Troponins are contractile proteins that areAbility of soluble Troponins toAnti-angiogenesisfibrillarAccessionthought to inhibit angiogenesis. High levelsinhibit anglogenesis can beproteinW18054may contribute to the difficulty encountereddetermined using assays known introponin Iin revascularizing the ischemic myocardiumthe art:. Proc Natl Acad Sci USAafter cardiovascular injury.1999 Mar. 16; 96(6):2645-50.TroponinGeneSeqWO9933874Troponins are contractile proteins that areAbility of soluble Troponins toAnti-angiogenesispeptidesAccessionsthought to inhibit angiogenesis. High levelsinhibit anglogenesis can beY29581,may contribute to the difficulty encountereddetermined using assays known inY29582,in revascularizing the ischemic myocardiumthe art:. Proc Natl Acad Sci USAY29583,after cardiovascular injury.1999 Mar. 16; 96(6):2645-50.Y29584,Y29585,andY29586Human fastGeneSeqWO0054770Troponins are contractile proteins that areAbility of soluble Troponins toAnti-angiogenesistwitchAccessionthought to inhibit angiogenesis. High levelsinhibit anglogenesis can beskeletalB00134may contribute to the difficulty encountereddetermined using assays known inmusclein revascularizing the ischemic myocardiumthe art:. Proc Natl Acad Sci USATroponinafter cardiovascular injury.1999 Mar. 16; 96(6):2645-50.subunit CHuman fastGeneSeqWO0054770Troponins are contractile proteins that areAbility of soluble Troponins toAnti-angiogenesistwitchAccessionthought to inhibit angiogenesis. High levelsinhibit anglogenesis can beskeletalB00135may contribute to the difficulty encountereddetermined using assays known inmusclein revascularizing the ischemic myocardiumthe art:. Proc Natl Acad Sci USATroponinafter cardiovascular injury.1999 Mar. 16; 96(6):2645-50.subunit IProteinHuman fastGeneSeqWO0054770Troponins are contractile proteins that areAbility of soluble Troponins toAnti-angiogenesistwitchAccessionthought to inhibit angiogenesis. High levelsinhibit anglogenesis can beskeletalB00136may contribute to the difficulty encountereddetermined using assays known inmusclein revascularizing the ischemic myocardiumthe art:. Proc Natl Acad Sci USATroponinafter cardiovascular injury.1999 Mar. 16; 96(6):2645-50.subunit TActivatorGeneSeqWO9013648PAIs are believed to play a roleMethods that measure plasminogenAnti-angiogenesis; blood-In-Accessionin cancer, and cardiovascular diseaseactivator inhibitor (PA1) activityclotting disorders.hibitor-1;R08411and blood-clotting disorders.are known in the art, for example,PAI-1assay the ability of PA1 to inhibittissue plasminogen activator (tPA)or urokinase (uPA): J BiochemBiophys Methods 2000 Sep. 11; 45(2):127-40, Breast Cancer ResTreat 1996; 41(2):141-6. Methodsthat measure anti-angiogenesisactivity are known in the art, forexample, Proc Natl Acad Sci USA1999 Mar. l6; 96(6):2645-50.Plasmin-GeneSeqDE3722673PAIs are believed to play a roleMethods that measure plasminogenAnti-angiogenesis; blood-ogenAccessionin cancer, and cardiovascular diseaseactivator inhibitor (PA1) activityclotting disorders.ActivatorP94160and blood-clotting disorders.are known in the art, for example,In-assay the ability of PA1 to inhibithibitor-2;tissue plasminogen activator (tPA)PAI-2or urokinase (uPA): J BiochemBiophys Methods 2000 Sep. 11; 45(2):127-40, Breast Cancer ResTreat 1996; 41(2): 141-6. Methodsthat measure anti-angiogenesisactivity are known in the art, forexample, Proc Natl Acad Sci USA1999 Mar. l6; 96(6):2645-50.ActivatorGeneSeqWO9102057PAIs are believed to play a roleMethods that measure plasminogenAnti-angiogenesis; blood-In-Accessionin cancer, and cardiovascular diseaseactivator inhibitor (PA1) activityclotting disorders.hibitor-2;R10921and blood-clottingare known in the art, for example,PAI-2disorders.assay the ability of PA1 to inhibittissue plasminogen activator (tPA)or urokinase (uPA): J BiochemBiophys Methods 2000 Sep. 11; 45(2):127-40, Breast Cancer ResTreat 1996; 41(2):141-6. Methodsthat measure anti-angiogenesisactivity are known in the art, forexample, Proc Natl Acad Sci USA1999 Mar. 16; 96(6):2645-50.HumanGeneSeqWO9105048PAIs are believedMethods that measure plasminogenAnti-angiogenesis; blood-PAI-1Accessionsto play a role inactivator inhibitor (PA1) activityclotting disorders.mutantsR11755,cancer, and cardio-are known in the art, for example,R11756,vascular diseaseassay the ability of PA1 to inhibitR11757,and blood-clottingtissue plasminogen activator (tPA)R11758,disorders.or urokinase (uPA): J BiochemR11759,Biophys Methods 2000 Sep. 11; 45(2):R11760,127-40, Breast Cancer ResR11761,Treat 1996; 41(2):141-6. MethodsR11762that measure anti-angiogenesisandactivity are known in the art, forR11763example, Proc Natl Acad Sci USA1999 Mar. 16; 96(6):2645-50.CXCR3;GeneSeqWO0018431Chemokines are a familyChemokine activities can beSoluble CXCR3 polypeptidesCXCAccessionof related small, secreted proteinsdetermined using assays known inmay be useful for inhibitingY79372involved in biological processesthe art: Methods in Molecularchemokine activities and viralranging from hematopoiesis,Biology, 2000, vol. 138:infection.angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.ModifiedGeneSeqWO9737005Chemokines are a familyChemokine activities can beImmune disorders.RantesAccessionof related small, secreted proteinsdetermined using assays known inW38129involved in biological processesthe art: Methods in Molecularranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.RANTESGeneSeqEP905240Chemokines are a familyChemokine activities can beImmune disorders.Accessionof related small, secreted proteinsdetermined using assays known inY05299involved in biological processesthe art: Methods in Molecularranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.MCI-1aGeneSeqWO9509232Chemokines are a familyChemokine activities can beImmune disorders.Accessionof related small, secreted proteinsdetermined using assays known inR73914involved in biological processesthe art: Methods in Molecularranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.MCP-1bGeneSeqWO9929728Chemokines are a familyChemokine activities can beImmune disorders.Accessionof related small, secreted proteinsdetermined using assays known inY26176involved in biological processesthe art: Methods in Molecularranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.MCP-1GeneSeqWO9519436Chemokines are a familyChemokine activities can beSoluble MCP-1 ReceptorreceptorAccessionof related small, secreted proteinsdetermined using assays known inpolypeptides may be useful forR79165involved in biological processesthe art: Methods in Molecularinhibiting chemokine activitiesranging from hematopoiesis,Biology, 2000, vol. 138:and viral infection.angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.MCP-3GeneSeqW09509232Chemokines are a familyChemokine activities can beImmune disorders.Accessionof related small, secreted proteinsdetermined using assays known inR73915involved in biological processesthe art: Methods in Molecularranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.MCP-4GeneSeqW09809171Chemokines are a familyChemokine activities can beSoluble MCP-4 ReceptorreceptorAccessionof related small, secreted proteinsdetermined using assays known inpolypeptides may be useful forW56689involved in biological processesthe art: Methods in Molecularinhibiting chemokine activitiesranging from hematopoiesis,Biology, 2000, vol. 138:and viral infection.angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.RANTESGeneSeqUS5652133Chemokines are a familyChemokine activities can beSoluble RANTES ReceptorreceptorAccessionof related small, secreted proteinsdetermined using assays known inpolypeptides may be useful forW29588involved in biological processesthe art: Methods in Molecularinhibiting chemokine activitiesranging from hematopoiesis,Biology, 2000, vol. 138:and viral infection.angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited byMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.CCR5GeneSeqWO9854317Chemokines are a familyChemokine activities can beSoluble CCR5 polypeptidesvariantAccessionof related small, secreted proteinsdetermined using assays known inmay be useful for inhibitingW88238involved in biological processesthe art: Methods in Molecularchemokine activities and viralranging from hematopoiesis,Biology, 2000, vol. 138:infection.angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.CCR7GeneSeqUS6153441Chemokines are a familyChemokine activities can beSoluble CCR7 polypeptidesAccessionof related small, secreted proteinsdetermined using assays known inmay be useful for inhibitingB50859involved in biological processesthe art: Methods in Molecularchemokine activities and viralranging from hematopoiesis,Biology, 2000, vol. 138:infection.angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.CXC3GeneSeqWO9727299Chemokines are a familyChemokine activities can beImmune disorders.Accessionof related small, secreted proteinsdetermined using assays known inW23345involved in biological processesthe art: Methods in Molecularranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.EotaxinGeneSeqWO9700960Chemokines are a familyChemokine activities can beImmune disorders.Accessionof related small, secreted proteinsdetermined using assays known inW10099involved in biological processesthe art: Methods in Molecularranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.Neuro-GeneSeqUS6013257Neurotactin may play a role in chemotacticChemotactic leukocyte migrationImmune disorders.tactinAccessionsWO9742224leukocyte migration and brain inflammationassays are known in the art, forY77537,processes.example: J. Immunol. Methods 33,W34307,(( 1980)); Nature 1997 Jun. 5;Y53259,387(6633):611-7.and,Y77539HumanGeneSeqUS6153441Chemokines are a familychemokine activities can beImmune disorders.CKbeta-9Accessionof related small, secreted proteinsdetermined using assays known inB50860involved in biological processesthe art: Methods in Molecularranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.Lympho-GeneSeqWO0073320Chemokines are a family of relatedchemokine activities can beImmune disorders.tactinAccessionsmall, secreted proteins involved indetermined using assays known inB50052biological processes ranging fromthe art: Methods in Molecularhematopoiesis, angiogenesis, andBiology, 2000, vol. 138:leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumor biology.The chemokines exert their effects by actingon a family of seven transmembrane G.MIP-3GeneSeqWO9801557Chemokines are a family of relatedchemokine activities can beImmune disorders.alphaAccessionsmall, secreted proteins involved indetermined using assays known inW44398biological processes ranging fromthe art: Methods in Molecularhematopoiesis, angiogenesis, andBiology, 2000, vol. 138:leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumor biology.The chemokines exert their effects by actingon a family of seven transmembrane G.MIP-3GeneSeqWO9801557Chemokines are a family of relatedChemokine activities can beImmune disorders.betaAccessionsmall, secreted proteins involved indetermined using assays known inW44399biological processes ranging fromthe art: Methods in Molecularhematopoiesis, angiogenesis, andBiology, 2000, vol. 138:leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumor biology.The chemokines exert their effects by actingon a family of seven transmembrane G.MIP-GammaGeneSeqWO9504158Chemokines are a family of relatedChemokine activities can beImmune disorders.Accessionsmall, secreted proteins involved indetermined using assays known inR70798biological processes ranging fromthe art: Methods in Molecularhematopoiesis, angiogenesis, andBiology, 2000, vol. 138:leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumor biology.The chemokines exert their effects by actingon a family of seven transmembrane G.Stem CellGeneSeqWO9104274Chemokines are a family of relatedChemokine activities can beHematopoietic growth factors.Inhib-Accessionsmall, secreted proteins involved indetermined using assays known initoryR11553biological processes ranging fromthe art: Methods in MolecularFactorhematopoiesis, angiogenesis, andBiology, 2000, vol. 138:leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumor biology.The chemokines exert their effects by actingon a family of seven transmembrane G.thrombo-GeneSeqWO9521920Thrombopoietin is involved in theThrombopoietin (TPO) can beHematopoietic growth factors.poietinAccessionregulation of the growth andassayed to determine regulation ofR79905differentiation ofgrowth and differentiation ofmegakaryocytes and preceptors thereof.megakaryocytes. Mol Cell Biol2001 Apr.; 21(8):2659-70; ExpHematol 2001 Jan.; 29(1):51-8 andwithin.c-kitGeneSeqEP992579 andC-kit ligan is thought to stimulate theChemokine activities can beHematopoietic growth factors.ligand;AccessionEP676470proliferation of mast cells, and is able todetermined using assays known inSCF; MastY53284,augment the proliferation of both myeloidthe art: Methods in MolecularcellR83978and lymphoid hematopoietic progenitors inBiology, 2000, vol. 138:growthandbone marrow culture. C-kit ligand is alsoChemokine Protocols. Edited by:factor;R83977though to act synergistically with otherA. E. I. Proudfoot, T. N. C. Wells,MGF;cytokines.and C. A. Power. © Humana PressFibro-Inc., Totowa, NJ.sarcoma-derivedstem cellfactorPlateletGeneSeqWO0066736Vascular Endothelial Growth FactorVEGF activity can be determinedPromotion of growth andderivedAccessionusing assays known in the art, suchproliferation of cells, such asgrowthB48653as those disclosed in Internationalvascular endothelial cells.factorPublication No. WO0045835, forAntagonists may be useful asexample.anti-angiogenic agents, andmay be applicable for cancer.MelanomaGeneSeqWO9503328Melanoma inhibiting protein has melanoma-Tumor suppressor activity ofCancer; melanomainhibitingAccessioninhibiting activity and can be used to treatmelanoma inhibiting protein can beproteinR69811cancer (melanoma, glioblastoma,determined using assays known inneuroblastoma, small cell lung cancer,the art: Matzuk et al., Nature 1992neuroectodermal tumors) or as anNov. 26; 360(6402):313-9.immunosuppressant (it inhibits IL-2 orphytohaemagglutinin induced proliferation ofperipheral blood lymphocytes.Glioma-GeneSeqEP399816Vascular Endothelial Growth FactorVEGF activity can be determinedPromotion of growth andderivedAccessionusing assays known in the art, suchproliferation of cells, such asgrowthR08120as those disclosed in Internationalvascular endothelial cells.factorPublication No. WO0045835, forAntagonists may be useful asexample.anti-angiogenic agents, andmay be applicable for cancer.PlateletGeneSeqEP682110VascularVEGF activity can be determinedPromotion of growth andderivedAccessionEndothelialusing assays known in the art, suchproliferation of cells, such asgrowthR84759Growth Factoras those disclosed in Internationalvascular endothelial cells.factorPublication No. WO0045835, forAntagonists may be useful aspre-example.anti-angiogenic agents, andcursor Amay be applicable for cancer.PlateletGeneSeqEP682110Vascular Endothelial Growth FactorVEGF activity can be determinedPromotion of growth andderivedAccessionusing assays known in the art, suchproliferation of cells, such asgrowthR84760as those disclosed in Internationalvascular endothelial cells.factorPublication No. WO0045835, forAntagonists may be useful aspre-example.anti-angiogenic agents, andcursor Bmay be applicable for cancer.PlateletGeneSeqEP282317Vascular Endothelial Growth FactorVEGF activity can be determinedPromotion of growth andderivedAccessionusing assays known in the art, suchproliferation of cells, such asgrowthP80595as those disclosed in Internationalvascular endothelial cells.factorandPublication No. WO0045835, forAntagonists may be useful asBv-sisP80596example.anti-angiogenic agents, andmay be applicable for cancer.PlacentalGeneSeqWO9206194Vascular Endothelial Growth FactorVEGF activity can be determinedPromotion of growth andGrowthAccessionsusing assays known in the art, suchproliferation of cells, such asFactorR23059as those disclosed in Internationalvascular endothelial cells.andPublication No. WO0045835, forAntagonists may be useful asR23060example.anti-angiogenic agents, andmay be applicable for cancer.PlacentalGeneSeqDE19748734Vascular Endothelial Growth FactorVEGF activity can be determinedPromotion of growth andGrowthAccessionusing assays known in the art, suchproliferation of cells, such asFactor-2Y08289as those disclosed in Internationalvascular endothelial cells.Publication No. WO0045835, forAntagonists may be useful asexample.anti-angiogenic agents, andmay be applicable for cancer.Thrombo-GeneSeqWO0000612Thrombopoietin is involvedThrombopoietin (TPO) can beThrombocytopenia, cancer.poietinAccessionin the regulation of the growth andassayed to determine regulation ofderiv-Y77244differentiation ofgrowth and differentiation ofative1megakaryocytes and preceptors thereof.megakaryocytes. Mol Cell Biol2001 Apr.; 21(8):2659-70; ExpHematol 2001 Jan.; 29(1):51-8 andwithin.Thrombo-GeneSeqWO0000612Thrombopoietin is involvedThrombopoietin (TPO) can beThrombocytopenia, cancer.poietinAccessionin the regulation of the growth andassayed to determine regulation ofderiv-Y77255differentiation ofgrowth and differentiation ofative2megakaryocytes and preceptors thereof.megakaryocytes. Mol Cell Biol2001 Apr.; 21(8):2659-70; ExpHematol 2001 Jan.; 29(1):51-8 andwithin.Thrombo-GeneSeqWO0000612Thrombopoietin is involvedThrombopoietin (TPO) can beThrombocytopenia, cancer.poietinAccessionin the regulation of the growth andassayed to determine regulation ofderiv-Y77262differentiation ofgrowth and differentiation ofative3megakaryocytes and preceptors thereof.megakaryocytes. Mol Cell Biol2001 Apr.; 21(8):2659-70; ExpHematol 2001 Jan.; 29(1):51-8 andwithin.Thrombo-GeneSeqWO0000612Thrombopoietin is involvedThrombopoietin (TPO) can beThrombocytopenia, cancer.poietinAccessionin the regulation of the growth andassayed to determine regulation ofderiv-Y77267differentiation ofgrowth and differentiation ofative4megakaryocytes and preceptors thereof.megakaryocytes. Mol Cell Biol2001 Apr.; 21(8):2659-70; ExpHematol 2001 Jan.; 29(1):51-8 andwithin.Thrombo-GeneSeqWO0000612Thrombopoietin is involvedThrombopoietin (TPO) can beThrombocytopenia, cancer.poietinAccessionin the regulation of the growth andassayed to determine regulation ofderiv-Y77246differentiation ofgrowth and differentiation ofative5megakaryocytes and preceptors thereof.megakaryocytes. Mol Cell Biol2001 Apr.; 21(8):2659-70; ExpHematol 2001 Jan.; 29(1):51-8 andwithin.Thrombo-GeneSeqWO0000612Thrombopoietin is involvedThrombopoietin (TPO) can beThrombocytopenia, cancer.poietinAccessionin the regulation of the growth andassayed to determine regulation ofderiv-Y77253differentiation ofgrowth and differentiation ofative6megakaryocytes and preceptors thereof.megakaryocytes. Mol Cell Biol2001 Apr.; 21(8):2659-70; ExpHematol 2001 Jan.; 29(1):51-8 andwithin.Thrombo-GeneSeqWO0000612Thrombopoietin is involvedThrombopoietin (TPO) can beThrombocytopenia, cancer.poietinAccessionin the regulation of the growth andassayed to determine regulation ofderiv-Y77256differentiation ofgrowth and differentiation ofative7megakaryocytes and preceptors thereof.megakaryocytes. Mol Cell Biol2001 Apr.; 21(8):2659-70; ExpHematol 2001 Jan.; 29(1):51-8 andwithin.Fract-GeneSeqUS6043086Fractalkine is believed to play a role inFractalkine activity can beImmune disorders.alkineAccessionchemotactic leukocyte migration anddetermined using ChemotacticY53255neurological disorders.leukocyte migration assays knownin the art, for example: J.Immunol. Methods 33, ((1980)); Nature1997 Jun. 5; 387(6633):611-7.CXC3GeneSeqWO9757599Chemokines are a familyChemokine activities can beImmune disorders.Accessionof related small, secreted proteinsdetermined using assays known inW23345involved in biological processesthe art: Methods in Molecularranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.CCR7GeneSeqUS6153441Chemokines are a familyChemokine activities can beSoluble CCR7 polypeptidesAccessionof related small, secreted proteinsdetermined using assays known inmay be useful for inhibitingB50859involved in biological processesthe art: Methods in Molecularchemokine activities and viralranging from hematopoiesis,Biology, 2000, vol. 138:infection.angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ.rejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.NerveGeneSeqEP414151Nerve Growth FactorProliferation assay using NR6R-Neurological disorders, cancerGrowthAccession3T3 cells (Rizzino 1988 CancerFactor-R11474Res. 48: 4266)betaNerveGeneSeqEP859056Nerve Growth FactorProliferation assay using NR6R 3T3Neurological disorders, cancerGrowthAccessioncells (Rizzino 1988 Cancer Res. 48:Factor-W697254266beta2Neuro-GeneSeqWO9821234Neurotrophins regulate neuronalTrk tyrosine kinase activation assaysNeurological disorders, cancertrophin-3Accessioncell survivalknown in the art can be used toW8889and synaptic plasticity.assay for neurotrophin activity, forexample, Proc Natl Acad Sci USA2001 Mar. 13; 98(6):3555-3560.Neuro-GeneSeqWO9325684Neurotrophins regulate neuronalTrk tyrosine kinase activation assaysNeurological disorders, cancertrophin-3Accessioncell survivalknown in the art can be used toR47100and synaptic plasticity.assay for neurotrophin activity, forexample, Proc Natl Acad Sci USA2001 Mar. 13; 98(6):3555-3560.Neuro-GeneSeqWO9325684Neurotrophins regulate neuronalTrk tyrosine kinase activation assaysNeurological disorders, cancertrophin-4aAccessioncell survivalknown in the art can be used toR47101and synaptic plasticity.assay for neurotrophin activity, forexample, Proc Natl Acad Sci USA2001 Mar. 13; 98(6):3555-3560.13; 98(6):3555-3560Neuro-GeneSeqWO9325684Neurotrophins regulate neuronalTrk tyrosine kinase activation assaysNeurological disorders, cancertrophin-4bAccessioncell survival and synapticknown in the art can be used toR47102plasticity, tyrosine kinases.assay for neurotrophin activity, forexample, Proc Natl Acad Sci USA2001 Mar. 13; 98(6):3555-3560.Neuro-GeneSeqWO9325684Neurotrophins regulate neuronalTrk tyrosine kinase activation assaysNeurological disorders, cancertrophin-4cAccessioncell survival and synapticknown in the art can be used toR47103plasticity, tyrosine kinases.assay for neurotrophin activity, forexample, Proc Natl Acad Sci USA2001 Mar. 13; 98(6):3555-3560.Neuro-GeneSeqWO9325684Neurotrophins regulate neuronalTrk tyrosine kinase activation assaysNeurological disorders, cancertrophin-4dAccessioncell survival and synapticknown in the art can be used toR47102plasticity, tyrosine kinases.assay for neurotrophin activity, forexample, Proc Natl Acad Sci USA2001 Mar. 13; 98(6):3555-3560.Platelet-GeneSeqUS5219739Vascular Endothelial Growth FactorVEGF activity can be determinedPromotion of growth andDerivedAccessionusing assays known in the art, suchproliferation of cells, such asGrowthR38918as those disclosed in Internationalvascular endothelial cells.FactorPublication No. W00045835, forHematopoietic and immuneA chainexample.disorders. Antagonists may beuseful as anti-angiogenicagents, and may be applicablefor cancerPlatelet-GeneSeqUS5219739Vascular Endothelial Growth FactorVEGF activity can be determinedPromotion of growth andDerivedAccessionusing assays known in the art, suchproliferation of cells, such asGrowthR38919as those disclosed in Internationalvascular endothelial cells.FactorPublication No. W00045835, forHematopoietic and immuneB chainexample.disorders. Antagonists may beuseful as anti-angiogenicagents, and may be applicablefor cancerStromalGeneSeqWO9948528Stromal Growth FactorProliferation assay using NR6R-3T3Hematopoietic, immuneDerivedAccessioncells (Rizzino 1988 Cancer Res. 48:disorders, cancerFactor-1Y399954266)alphaStromalGeneSeqCA2117953Stromal Growth FactorProliferation assay using NR6R-3T3Hematopoietic, immuneDerivedAccessioncells (Rizzino 1988 Cancer Res. 48:disorders, cancerFactor-1R754204266)betaTarcGeneSeqWO9711969Chemotactic for T lymphocytes. MayChemotactic leukocyte migrationAntiinflammatory. ImmuneAccessionplay a role in T-cell development.assays are known in the art, fordisorders, cancerW14917Thought to bind CCR8 and CCR4example: J. Immunol. Methods 33((1980))Pro-GeneSeqWO9521625Prolactin is involved in immune cellImmune coil proliferation andReproductive systemlactinAccessionproliferation and apoptosis.suppression of apoptosis bydisorders, cancer.R78691prolactin can be assayed by methodswell-known in the art, for example,Buckley, AR and Buckley DJ, AnnN Y Acad Sci 2000; 917:522-33,and within.Pro-GeneSeqUS5955346Prolactin is involved in immune cellImmune coil proliferation andReproductive systemlactin2Accessionproliferation and apoptosis.suppression of apoptosis bydisorders, cancer.Y31764prolactin can be assayed by methodswell-known in the art, for example,Buckley, AR and Buckley DJ, AnnNY Acad Sci 2000; 917:522-33,and within.FollicleGeneSeqEP974359FSH stimulates secretion of interleukin-1 byFSH activities can be determinedReproductive systemstimu-Accessioncells isolated from women in the follicularusing assays known in the art; Jdisorders, cancer.latingY54160phaseGend Specif Med 1999 Nov.-hormoneDec.; 2(6):30-4; Mol CellAlphaEndocrinol. 1997 Nov. l5;subunit134(2):109-18.FollicleGeneSeqEP974359FSH stimulates secretion of interleukin-1 byFSH activities can be determinedReproductive systemstimu-Accessioncells isolated from women in the follicularusing assays known in the art; Jdisorders, cancer.latingY54161phaseGend Specif Med 1999 Nov.-hormoneDec.; 2(6):30-4; Mol CellBetaEndocrinol. 1997 Nov. l5;subunit134(2):109-18.Sub-GeneSeqWO0054053Substance P is associated withImmuneregulation and bonediabetes mellitus,stance PAccessionimmunoregulation.marrow, cell proliferation byhypertension, cancer(tachy-B23027substance P can be assayed bykinin)methods well-known in the art, forexample, Lai et al. Proc Natl AcadSci USA 2001 Mar. 27; 98(7):3970-5; Jallat-Daloz et al. Allergy AsthmaProc 2001 Jan.-Feb.; 22(1):17-23; Kahler et al. Exp LungRes 2001Jan.-Feb.; 27(1):25-46; andAdamus M A and Dabrowski Z J. J CellBiochem 2001; 81(3)499-506.OcytocinGeneSeqWO0053755Oxytocin is involved in the induction ofOxytocin and prostaglandin E(2)inflammatory disorders(Neuro-Accessionprostaglandin (E2) release as well as anrelease and Ocytocin (Ca2+)immunologic disorders, cancerphysin I)B24085increased amount of calcium release byincrease can be assayed by methodsandsmooth muscle cells.well-known in the art, for example,B24086Pavan et al., AM J Obset Gynecol2000 Jul.; 183(1):76-82 andHoldaet al., Cell Calcium 1996Jul.; 20(1):43 51.Vaso-GeneSeqWO0053755Vasopressinis believed to have a directVasopressin activity can beinflammatory disorderspressinAccessionantidiuretic action on the kidney, and it isdetermined using assays known inimmunologic disorders, cancer(Neuro-B24085thought to cause vasoconstriction of thethe art, for example, Endocr Regulphysinandperipheral vessels.1996 Mar.; 30(1):13-17.II)B24086IL-1GeneSeqEP165654Interleukins are a groupInterleukin activity can beinflammatory disorders,Accessionof multifunctionaldetermined using assays known inimmunologicP60326cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Orencole &and lymphocytes), chemotaxisDinarclio (1989) Cytokine 1, 14-20.of neutrophils and T lymphocytes,and/or inhibition of interferons.IL-1-GeneSeqEP456332Interleukins are a groupInterleukin activity can beinflammatory disorders,matureAccessionof multifunctionaldetermined using assays known inimmunologicR14855cytokines synthesized by lymphocytes,the art: Matthews et al, indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Orencole &and lymphocytes), chemotaxisDinarclio (1989) Cytokine 1, 14-20.of neutrophils and T lymphocytes,and/or inhibition of interferons.IL-1GeneSeqWO9922763Interleukins are a groupInterleukin activity can beinflammatory disorders,betaAccessionof multifunctionaldetermined using assays known inimmunologicY08322cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens. Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Orencole &and lymphocytes), chemotaxisDinarclio (1989) Cytokine 1, 14-20.of neutrophils and T lymphocytes,and/or inhibition of interferons.IL-3GeneSeqWO8806161Interleukins are a groupInterleukin activity can beinflammatory disorders,variantsAccessionof multifunctionaldetermined using assays known inimmunologicP80382,cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerP80383,monocytes, and macrophages. KnownLymphokines and Interferens: AP80384,functions include stimulatingPractical Approach, Clemens et al.,andproliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.P80381T helper cells, B cells, eosinophils,1987, pp. 221-225; and Kitamuraand lymphocytes), chemotaxiset al (1989) J Cell Physiol.of neutrophils and T lymphocytes,140 323-334.and/or inhibition of interferons.IL-4GeneSeqWO8702990Interleukins are a groupInterleukin activity can beinflammatory disorders,Accessionof multifunctionaldetermined using assays known inimmunologicP70615cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Siegel &and lymphocytes), chemotaxisMostowski (1990) J Immunolof neutrophils and T lymphocytes,Methods 132, 287-295.and/or inhibition of interferons.IL-4GeneSeqWO9747744Interleukins are a groupInterleukin activity can beinflammatory disorders,muteinsAccessionof multifunctionaldetermined using assays known inimmunologicW52151cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerW52152monocytes, and macrophages. KnownLymphokines and Interferens: AW52153functions include stimulatingPractical Approach, Clemens et al.,W52154proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.W52155T helper cells, B cells, eosinophils,1987, pp. 221-225; and Siegel &W52156and lymphocytes), chemotaxisMostowski (1990) J ImmunolW52157of neutrophils and T lymphocytes,Methods 132, 287-295.W52158and/or inhibition of interferons.W52159W52160W52161W52162W52163W52164andW52165IL-1GeneSeqEP324447Interleukins are a groupInterleukin activity can beinflammatory disorders,alphaAccessionof multifunctionaldetermined using assays known inimmunologicP90108cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens. Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Orencole &and lymphocytes), chemotaxisDinarello (1989) Cytokine 1, 14-20.of neutrophils and T lymphocytes,and/or inhibition of interferons.IL-3GeneSeqWO9307171Interleukins are a groupInterleukin activity can beinflammatory disorders,variantsAccessionof multifunctionaldetermined using assays known inimmunologicR38561,cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerR38562,monocytes, and macrophages. KnownLymphokines and Interferens: AR38563,functions include stimulatingPractical Approach, Clemens et al.,R38564,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.R38565,T helper cells, B cells, eosinophils,1987, pp. 221-225; and AardenR38566,and lymphocytes), chemotaxiset al (1987) Eur. J. Immunol 17,R38567,of neutrophils and T lymphocytes,1411-16.R38568,and/or inhibition of interferons.R38569,R38570,R38571,andR38572IL-6GeneSeqWO9402512Interleukins are a groupInterleukin activity can beinflammatory disorders,Accessionof multifunctionaldetermined using assays known inimmunologicR45717cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerandmonocytes, and macrophages. KnownLymphokines and Interferens: AR45718functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Aardenand lymphocytes), chemotaxiset al (1987) Eur. J. Immunol 17,of neutrophils and T lymphocytes,1411-16.and/or inhibition of interferons.IL-13GeneSeqWO9404680Interleukins are a groupInterleukin activity can beinflammatory disorders,Accessionof multifunctionaldetermined using assays known inimmunologicR48624cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens. Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Boutelierand lymphocytes), chemotaxiset al (1995) J. Immunol. Methodsof neutrophils and T lymphocytes,181, 29.and/or inhibition of interferons.IL-4GeneSeqDE4137333Interleukins are a groupInterleukin activity can beinflammatory disorders,muteinAccessionof multifunctionaldetermined using assays known inimmunologicR47182cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Siegel &and lymphocytes), chemotaxisMostowski (1990) J Immunolof neutrophils and T lymphocytes,Methods 132, 287-295.and/or inhibition of interferons.IL-4GeneSeqDE4137333Interleukins are a groupInterleukin activity can beinflammatory disorders,muteinAccessionof multifunctionaldetermined using assays known inimmunologicY124XR47183cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Siegel &and lymphocytes), chemotaxisMostowski (1990) J Immunolof neutrophils and T lymphocytes,Methods 132, 287-295.and/or inhibition of interferons.IL-4GeneSeqDE4137333Interleukins are a groupInterleukin activity can beinflammatory disorders,muteinAccessionof multifunctionaldetermined using assays known inimmunologicY124GR47184cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens. Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Siegel &and lymphocytes), chemotaxisMostowski (1990) J Immunolof neutrophils and T lymphocytes,Methods 132, 287-295.and/or inhibition of interferons.HumanGeneSeqWO9317698Interleukins are a groupInterleukin activity can beinflammatory disorders,Inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin-10R41664cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancer(pre-monocytes, and macrophages. KnownLymphokines and Interferens: Acursor)functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Thompson-and lymphocytes), chemotaxisSnipes et al (1991) J. Exp. Med.of neutrophils and T lymphocytes,173, 507-510.and/or inhibition of interferons.HumanGeneSeqWO9318783-AInterleukins are a groupInterleukin activity can beinflammatory disorders,Inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin-10R42642cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Thompson-and lymphocytes), chemotaxisSnipes et al (1991) J. Exp. Med.of neutrophils and T lymphocytes,173, 507-510.and/or inhibition of interferons.HumanGeneSeqEP569042Interleukins are a groupInterleukin activity can beinflammatory disorders,inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin-1R42447cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerbetamonocytes, and macrophages. KnownLymphokines and Interferens: Aprecursor.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Orencole &and lymphocytes), chemotaxisDinarello (1989) Cytokine 1, 14-20.of neutrophils and T lymphocytes,and/or inhibition of interferons.Inter-GeneSeqEP578278Interleukins are a groupInterleukin activity can beinflammatory disorders,leukin-Accessionof multifunctionaldetermined using assays known inimmunologic1 alphaR45364cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225.and lymphocytes), chemotaxisof neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqJP04063595Interleukins are a groupInterleukin activity can beinflammatory disorders,inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin-3R22814cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancervariantmonocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Kitamuraand lymphocytes), chemotaxiset al (1989) J Cell Physiol. 140of neutrophils and T lymphocytes,323-334.and/or inhibition of interferons.IL-1iGeneSeqEP541920Interleukins are a groupInterleukin activity can beinflammatory disorders,fragmentsAccessionof multifunctionaldetermined using assays known inimmunologicR35484cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerandmonocytes, and macrophages. KnownLymphokines and Interferens: AR35485functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; andand lymphocytes), chemotaxisOrencole & Dinarclio (1989)of neutrophils and T lymphocytes,Cytokine 1, 14-20.and/or inhibition of interferons.IL-1GeneSeqEPS541920Interleukins are a groupInterleukin activity can beinflammatory disorders,inhibitorAccessionof multifunctionaldetermined using assays known inimmunologic(IL-1i)R35486cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerandmonocytes, and macrophages. KnownLymphokines and Interferens: AR35484functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; andand lymphocytes), chemotaxisOrencole & Dinarelio (1989)of neutrophils and T lymphocytes,Cytokine 1, 14-20.and/or inhibition of interferons.ICE 22 kDGeneSeqEP533350Interleukins are a groupInterleukin activity can beinflammatory disorders,subunit.Accessionof multifunctionaldetermined using assays known inimmunologicR33780cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225.and lymphocytes), chemotaxisof neutrophils and T lymphocytes,and/or inhibition of interferons.ICE 20 kDGeneSeqEP533350Interleukins are a groupInterleukin activity can beinflammatory disorders,subunit.Accessionof multifunctionaldetermined using assays known inimmunologicR33781cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225.and lymphocytes), chemotaxisof neutrophils and T lymphocytes,and/or inhibition of interferons.ICE 10 kDGeneSeqEP533350Interleukins are a groupInterleukin activity can beinflammatory disorders,subunitAccessionof multifunctionaldetermined using assays known inimmunologicR33782cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225.and lymphocytes), chemotaxisof neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqWO9317698Interleukins are a groupInterleukin activity can beinflammatory disorders,Inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin-10R41664cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancer(precursor)monocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Thompson-and lymphocytes), chemotaxisSnipes et al (1991) J. Exp. Med.of neutrophils and T lymphocytes,173, 507-510.and/or inhibition of interferons.HumanGeneSeqWO9318783Interleukins are a groupInterleukin activity can beinflammatory disorders,Inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin-10R42642cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens. Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Thompson-and lymphocytes), chemotaxisSnipes et al (1991) J. Exp. Med.of neutrophils and T lymphocytes,173, 507-510.and/or inhibition of interferons.HumanGeneSeqEP569042Interleukins are a groupInterleukin activity can beinflammatory disorders,Inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin-1R42447cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerbetamonocytes, and macrophages. KnownLymphokines and Interferens: Aprecursorfunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Kitamuraand lymphocytes), chemotaxiset al (1989) J Cell Physiol. 140of neutrophils and T lymphocytes,323-334.and/or inhibition of interferons.HumanGeneSeqWO9403492Interleukins are a groupInterleukin activity can beinflammatory disorders,inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin-6R49041cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Aarden et aland lymphocytes), chemotaxis(1987) Eur. J. Immunol 17, 1411-16.of neutrophils and T lymphocytes,and/or inhibition of interferons.MutantGeneSeqWO9411402Interleukins are a groupInterleukin activity can beinflammatory disorders,Inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin 6R54990cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerS176Rmonocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Aarden et aland lymphocytes), chemotaxis(1987) Eur. J. Immunol 17, 1411-16.of neutrophils and T lymphocytes,and/or inhibition of interferons.Inter-GeneSeqJP06145063Interleukins are a groupInterleukin activity can beinflammatory disorders,leukin 6Accessionof multifunctionaldetermined using assays known inimmunologicR55256cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens. Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Aarden et aland lymphocytes), chemotaxis(1987) Eur. J. Immunol 17, 1411-16.of neutrophils and T lymphocytes,and/or inhibition of interferons.Inter-GeneSeqJP06100595Interleukins are a groupInterleukin activity can beSoluble IL-8 receptorleukin 8Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful for(IL-8)R53932cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukin activities.receptormonocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Holmes et aland lymphocytes), chemotaxis(1991) Science 253, 1278-80.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqUS5328988Interleukins are a groupInterleukin activity can beinflammatory disorders,inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin-7R59919cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens. Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Park et aland lymphocytes), chemotaxis(1990) J. Exp. Med. 171, 1073-79.of neutrophils and T lymphocytes,and/or inhibition of interferons.IL-3GeneSeqWO9521254Interleukins are a groupInterleukin activity can beinflammatory disorders,containingAccessionof multifunctionaldetermined using assays known inimmunologicfusionR79342cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerprotein.andmonocytes, and macrophages. KnownLymphokines and Interferens: AR79344functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Kitamuraand lymphocytes), chemotaxiset al (1989) J Cell Physiol. 140 323-of neutrophils and T lymphocytes,334.and/or inhibition of interferons.IL-3GeneSeqZA9402636Interleukins are a groupInterleukin activity can beinflammatory disorders, immunologicmutantAccessionof multifunctionaldetermined using assays known indisorders, cancerproteinsR79254,cytokines synthesized by lymphocytes,the art: Matthews et al., inR79255,monocytes, and macrophages. KnownLymphokines and Interferens: AR79256,functions include stimulatingPractical Approach, Clemens et al.,R79257,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.R79258,T helper cells, B cells, eosinophils,1987, pp. 221-225; and Giri et alR79259,and lymphocytes), chemotaxis(1994) EMBO J. 13 2822-2830.R79260,of neutrophils and T lymphocytes,R79261,and/or inhibition of interferons.R79262,R79263,R79264,R79265,R79266,R79267,R79268,R79269,R79270,R79271,R79272,R79273,R79274,R79275,R79276,R79277,R79278,R79279,R79280,R79281,R79282,R79283,R79284,andR79285IL-12 p40GeneSeqAU9466072Interleukins are a groupInterleukin activity can beinflammatory disorders,subunit.Accessionof multifunctionaldetermined using assays known inimmunologicR63018cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225.and lymphocytes), chemotaxisof neutrophils and T lymphocytes,and/or inhibition of interferons.AGFGeneSeqWO9429344Interleukins are a groupInterleukin activity can beinflammatory disorders,Accessionof multifunctionaldetermined using assays known inimmunologicR64240cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225.and lymphocytes), chemotaxisof neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqWO9519786Interleukins are a groupInterleukin activity can beinflammatory disorders,inter-Accessionof multifunctionaldetermined using assays known inimmunologiclaukin-12R79187cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancer40 kDmonocytes, and macrophages. KnownLymphokines and Interferens: Asubunitfunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Hori et aland lymphocytes), chemotaxis(1987), Blood 70, 1069-1078.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqWO9530695Interleukins are a groupInterleukin activity can beSoluble IL-8 receptorinter-Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful forleukin-15R90843cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinreceptormonocytes, and macrophages. KnownLymphokines and Interferens. Aactivities.fromfunctions include stimulatingPractical Approach, Clemens et al.,clone P1proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Giri et aland lymphocytes), chemotaxis(1994) EMBO J. 13 2822-2830.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqWO9604306Interleukins are a groupInterleukin activity can beinflammatory disorders,inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin-7R92796cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Park et aland lymphocytes), chemotaxis(1990) J. Exp. Med. 171, 1073-79.of neutrophils and T lymphocytes,and/or inhibition of interferons.inter-GeneSeqWO9604306Interleukins are a groupInterleukin activity can beinflammatory disorders,leukin-9Accessionof multifunctionaldetermined using assays known inimmunologicR92797cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Yang et aland lymphocytes), chemotaxis(1989) Blood 74, 1880-84.of neutrophils and T lymphocytes,and/or inhibition of interferons.inter-GeneSeqWO9604306Interleukins are a groupInterleukin activity can beinflammatory disorders,leukin-3Accessionof multifunctionaldetermined using assays known inimmunologicR92801cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Kitamuraand lymphocytes), chemotaxiset al (1989) J Cell Physiol. 140of neutrophils and T lymphocytes,323-334.and/or inhibition of interferons.HumanGeneSeqWO9604306Interleukins are a groupInterleukin activity can beinflammatory disorders,inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin-5R92802cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Kitamuraand lymphocytes), chemotaxiset al (1989) J Cell Physiol. 140of neutrophils and T lymphocytes,323-334.and/or inhibition of interferons.Recomb-GeneSeqDEI9617202Interleukins are a groupInterleukin activity can beinflammatory disorders,inantAccessionof multifunctionaldetermined using assays known inimmunologicinter-W33373cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerleukin-16monocytes, and macrophages. KnownLymphokines and Interferens. Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Lim et aland lymphocytes), chemotaxis(1996) J. Immunol. 156, 2566-70.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqDE19617202Interleukins are a groupInterleukin activity can beinflammatory disorders,IL-16Accessionof multifunctionaldetermined using assays known inimmunologicproteinW33234cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Lim et aland lymphocytes), chemotaxis(1996) J. Immunol. 156, 2566-70.of neutrophils and T lymphocytes,and/or inhibition of interferons.Thr1 17GeneSeqWO9708321Interleukins are a groupInterleukin activity can beinflammatory disorders,humanAccessionof multifunctionaldetermined using assays known inimmunologicinter-W27521cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerleukin 9monocytes, and macrophages. KnownLymphokines and Interferens. Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225.and lymphocytes), chemotaxisof neutrophils and T lymphocytes,and/or inhibition of interferons.Metl 17GeneSeqWO9708321Interleukins are a groupInterleukin activity can beinflammatory disorders,humanAccessionof multifunctionaldetermined using assays known inimmunologicinter-W27522cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerleukin 9monocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Yang et aland lymphocytes), chemotaxis(1989) Blood 74, 1880-84.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqEP86-4585Interleukins are a groupInterleukin activity can beinflammatory disorders,intra-Accessionof multifunctionaldetermined using assays known inimmunologiccellularW77158cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerIL-1monocytes, and macrophages. KnownLymphokines and Interferens: Areceptorfunctions include stimulatingPractical Approach, Clemens et al.,antagonist.proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Orencole &and lymphocytes), chemotaxisDinarello (1989) Cytokine 1, 14-20.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqEP864585Interleukins are a groupInterleukin activity can beinflammatory disorders,inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin-18W77158cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerproteinmonocytes, and macrophages. KnownLymphokines and Interferens: A(IL-18)functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and USHIO et aland lymphocytes), chemotaxis(1996) J. Immunol. 156, 4274-79.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqEP861663Interleukins are a groupInterleukin activity can beinflammatory disorders,inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin-18W77077cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferens: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and USHIO et aland lymphocytes), chemotaxis(1996) J. Immunol. 156, 4274-79.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqEP861663Interleukins are a groupInterleukin activity can beinflammatory disorders,inter-Accessionsof multifunctionaldetermined using assays known inimmunologicleukin 18W77083,cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerderiv-W77084,monocytes, and macrophages. KnownLymphokines and Interferons: AativesW77085,functions include stimulatingPractical Approach, Clemens et al.,W77086,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.W77087,T helper cells, B cells, eosinophils,1987, pp. 221-225; and Ushio et alW77088,and lymphocytes), chemotaxis(1996) J. Immunol, 156, 4274-79.andof neutrophils and T lymphocytes,W77089and/or inhibition of interferons.Inter-GeneSeqWO9827997Interleukins are a groupInterleukin activity can beinflammatory disorders,leukin-9Accessionof multifunctionaldetermined using assays known inimmunologic(IL-9)W68158cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermaturemonocytes, and macrophages. KnownLymphokines and Interferons: Aproteinfunctions include stimulatingPractical Approach, Clemens et al.,(Thr117proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.version).T helper cells, B cells, eosinophils,1987, pp. 221-225; and Yang et aland lymphocytes), chemotaxis(1989) Blood 74, 1880-84.of neutrophils and T lymphocytes,and/or inhibition of interferons.IL-9 matureGenSeqWO9827997Interleukins are a groupInterleukin activity can beinflammatory disorders,proteinAccessionof multifunctionaldetermined using assays known inimmunologicvariantW68157cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancer(Metl17monocytes, and macrophages. KnownLymphokines and Interferons: Aversion)functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Yang et aland lymphocytes), chemotaxis(1989) Blood 74, 1880-84.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqWO9824904Interleukins are a groupInterleukin activity can beinflammatory disorders,IL-9Accessionof multifunctionaldetermined using assays known inimmunologicreceptorW64058cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerproteinmonocytes, and macrophages. KnownLymphokines and Interferons. Avariant #3.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Yang et aland lymphocytes), chemotaxis(1989) Blood 74, 1880-84.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGenSeqWO9824904Interleukins are a groupInterleukin activity can beSoluble IL-9 receptorIL-9Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful forreceptorW64060cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinproteinmonocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.variantfunctions include stimulatingPractical Approach, Clemens et al.,fragmentproliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Yang et aland lymphocytes), chemotaxis(1989) Blood 74, 1880-84.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqWO9824904Interleukins are a groupInterleukin activity can beSoluble IL-9 receptorIL-9Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful forreceptorW64061cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinproteinmonocytes, and macrophages. KnownLymphokines and Interferons. Aactivities.variant #3.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Yang et aland lymphocytes), chemotaxis(1989) Blood 74, 1880-84.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqWO9817689Interleukins are a groupInterleukin activity can beinflammatory disorders,Inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin-12W51311cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerp40monocytes, and macrophages. KnownLymphokines and Interferons: Aproteinfunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Hori et aland lymphocytes), chemotaxis(1987), Blood 70, 1069-1078.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqWO9817689Interleukins are a groupInterleukin activity can beinflammatory disorders,inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin-12W51312cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerp35monocytes, and macrophages. KnownLymphokines and Interferons: Aproteinfunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Hori et aland lymphocytes), chemotaxis(1987), Blood 70, 1069-1078.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqDE19649233-Interleukins are a groupInterleukin activity can beinflammatory disorders,proteinAccessionof multifunctionaldetermined using assays known inimmunologicwithW63753cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerIL-16monocytes, and macrophages. KnownLymphokines and Interferons: Aactivityfunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Lim et aland lymphocytes), chemotaxis(1996) J. Immunol. 156, 2566-70.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqDE19649233-Interleukins are a groupInterleukin activity can beinflammatory disorders,proteinAccessionof multifunctionaldetermined using assays known inimmunologicwithW59425cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerIL-16monocytes, and macrophages. KnownLymphokines and Interferons: Aactivityfunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Lim et aland lymphocytes), chemotaxis(1996) J. Immunol. 156, 2566-70.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqUS5747024Interleukins are a groupInterleukin activity can beinflammatory disorders,inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin-W53878cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancer15monocytes, and macrophages. KnownLymphokines and Interferons: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Giri et aland lymphocytes), chemotaxis(1994) EMBO J. 13 2822-2830.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqWO9747744Interleukins are a groupInterleukin activity can beinflammatory disorders,wild-typeAccessionof multifunctionaldetermined using assays known inimmunologicinter-W52149cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerleukin-4monocytes, and macrophages. KnownLymphokines and Interferons: A(hIL-4)functions include stimulatingPractical Approach, Clemens et al.,proteinproliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Siegel &and lymphocytes), chemotaxisMostowski (1990) J Immunolof neutrophils and T lymphocytes,Methods 132, 287-295.and/or inhibition of interferons.inter-GeneSeqWO9747744Interleukins are a groupInterleukin activity can beinflammatory disorders,leukin-4Accessionsof multifunctionaldetermined using assays known inimmunologicmuteinsW52150,cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerW52151,monocytes, and macrophages. KnownLymphokines and Interferons: AW52153,functions include stimulatingPractical Approach, Clemens et al.,W52154,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.W52155,T helper cells, B cells, eosinophils,1987, pp. 221-225; and Siegel &W52156,and lymphocytes), chemotaxisMostowski (1990) J ImmunolW52157,of neutrophils and T lymphocytes,Methods 132, 287-295.W52158,and/or inhibition of interferons.W52159,W52160,W52161,W52162,W52163,W52164,W52165,W52166,andW52167HumanGeneSeqWO9935268Interleukins are a groupInterleukin activity can beinflammatory disorders,inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin 1Y28408cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerdeltamonocytes, and macrophages. KnownLymphokines and Interferons Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Orencole &and lymphocytes), chemotaxisDinarello (1989) Cytokine 1, 14-20.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqWO9935268Interleukins are a groupInterleukin activity can beinflammatory disorders,inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin-1Y24395cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerreceptormonocytes, and macrophages. KnownLymphokines and Interferons: Aantagonistfunctions include stimulatingPractical Approach, Clemens et al.,betaproliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Orencole &and lymphocytes), chemotaxisDinarello (1989) Cytokine 1, 14-20.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqWO9932632Interleukins are a groupInterleukin activity can beinflammatory disorders,EDIRF IIAccessionof multifunctionaldetermined using assays known inimmunologicproteinY22199cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancersequencemonocytes, and macrophages. KnownLymphokines and Interferons: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225.and lymphocytes), chemotaxisof neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqWO9932632Interleukins are a groupInterleukin activity can beinflammatory disorders,EDIRF IAccessionof multifunctionaldetermined using assays known inimmunologicproteinY22197cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancersequencemonocytes, and macrophages. KnownLymphokines and Interferons: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225.and lymphocytes), chemotaxisof neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqWO9919480Interleukins are a groupInterleukin activity can beSoluble IL-1RD10 receptorIL-1RD10Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful forproteinY14131cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinsequencemonocytes, and macrophages. KnownLymphokines and Interferons. Aactivites.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Orencole &and lymphocytes), chemotaxisDinarello (1989) Cytokine 1, 14-20.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqWO9919480Interleukins are a groupInterleukin activity can beSoluble IL-1RD10 receptorIL-1RD9Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful forY14122cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinmonocytes, and macrophages. KnownLymphokines and Interferons Aactivites.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Orencole &and lymphocytes), chemotaxisDinarello (1989) Cytokine 1, 14-20.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqWO9919491Interleukins are a groupInterleukin activity can beinflammatory disorders,DNAXAccessionof multifunctionaldetermined using assays known inimmunologicinter-Y09196cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerleukin-40monocytes, and macrophages. KnownLymphokines and Interferons. Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225.and lymphocytes), chemotaxisof neutrophils and T lymphocytes,and/or inhibition of interferons.(DIL-40)GeneSeqWO9919491Interleukins are a groupInterleukin activity can beinflammatory disorders,alternativeAccessionof multifunctionaldetermined using assays known inimmunologicsequenceY09197cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferons: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225.and lymphocytes), chemotaxisof neutrophils and T lymphocytes,and/or inhibition of interferons.IL-11GeneSeqWO9405318Interleukins are a groupInterleukin activity can beinflammatory disorders,Accessionof multifunctionaldetermined using assays known inimmunologicR50176cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferons: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Lu et aland lymphocytes), chemotaxis(1994) J immunol. Methods 173, 19.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqEP566410Interleukins are a groupInterleukin activity can beinflammatory disorders,adipo-Accessionof multifunctionaldetermined using assays known inimmunologicgenesisR43260cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerinhibitorymonocytes, and macrophages. KnownLymphokines and Interferons: Afactorfunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225.and lymphocytes), chemotaxisof neutrophils and T lymphocytes,and/or inhibition of interferons.IL-11GeneSeqJP08127539Interleukins are a groupInterleukin activity can beinflammatory disorders,Accessionof multifunctionaldetermined using assays known inimmunologicW02202cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferons: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Lu et aland lymphocytes), chemotaxis(1994) J immunol. Methods 173, 19.of neutrophils and T lymphocytes,and/or inhibition of interferons.IL-14GeneSeqWO9416074Interleukins are a groupInterleukin activity can beinflammatory disorders,Accessionof multifunctionaldetermined using assays known inimmunologicR55800cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferons: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Ambrus et aland lymphocytes), chemotaxis(1993) PNAS 90, 63330-34.of neutrophils and T lymphocytes,and/or inhibition of interferons.IL-17GeneSeqUS6072033Interleukins are a groupInterleukin activity can beSoluble IL-17 receptorreceptorAccessionof multifunctionaldetermined using assays known inpolypeptides may be useful forB03807cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinmonocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Yao et aland lymphocytes), chemotaxis(1995) J. Immunol. 155, 5483-86.of neutrophils and T lymphocytes,and/or inhibition of interferons.IL-17GeneSeqWO9518826Interleukins are a groupInterleukin activity can beinflammatory disorders,Accessionof multifunctionaldetermined using assays known inimmunologicR76573cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferons: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Yao et aland lymphocytes), chemotaxis(1995) J. Immunol. 155, 5483-86.of neutrophils and T lymphocytes,and/or inhibition of interferons.CTLA-8GeneSeqWO9704097Interleukins are a groupInterleukin activity can beinflammatory disorders,Accessionof multifunctionaldetermined using assays known inimmunologicW13651cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferons: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225.and lymphocytes), chemotaxisof neutrophils and T lymphocytes,and/or inhibition of interferons.IL-19GeneSeqWO9808870Interleukins are a groupInterleukin activity can beinflammatory disorders,Accessionof multifunctionaldetermined using assays known inimmunologicW37935cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferons: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Gallagher etand lymphocytes), chemotaxisal (2000) Genes Immun. 1, 442-50.of neutrophils and T lymphocytes,and/or inhibition of interferons.IL-21GeneSeqWO0024758Interleukins are a groupInterleukin activity can beinflammatory disorders,(TIF)Accessionof multifunctionaldetermined using assays known inimmunologicY92879cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferons: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Parrish-and lymphocytes), chemotaxisNovak et al (2000) Nature 408,of neutrophils and T lymphocytes,57-63.and/or inhibition of interferons.IL-8GeneSeqWO9306229Interleukins are a groupInterleukin activity can beSoluble IL-8 receptorreceptorAccessionof multifunctionaldetermined using assays known inpolypeptides may be useful forR33420cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinmonocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Holmes et aland lymphocytes), chemotaxis(1991) Science 253, 1278-80..of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqUS5464937Interleukins are a groupInterleukin activity can beSoluble type II interleukin-1type IIAccessionof multifunctionaldetermined using assays known inreceptor polypeptides may beinter-R85480cytokines synthesized by lymphocytes,the art: Matthews et al., inuseful for inhibitingleukin-1monocytes, and macrophages. KnownLymphokines and Interferons: Ainterleukinreceptorfunctions include stimulatingPractical Approach, Clemens et al.,activities.proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Orencole &and lymphocytes), chemotaxisDinarello (1989) Cytokine 1, 14-20.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqEP638644Interleukins are a groupInterleukin activity can beSoluble IL-12 receptorinter-Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful forleukin-12R69632cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinreceptormonocytes, and macrophages. KnownLymphokines and Interferons. Aactivities.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Hori et aland lymphocytes), chemotaxis(1987), Blood 70, 1069-1078.of neutrophils and T lymphocytes,and/or inhibition of interferons.Inter-GeneSeqUS5440021Interleukins are a groupInterleukin activity can beSoluble IL-8 receptor Bleukin 8Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful forreceptorR80758cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukin activities.Bmonocytes, and macrophages. KnownLymphokines and Interferons: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Holmes et aland lymphocytes), chemotaxis(1991) Science 253, 1278-80.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqJP08103276Interleukins are a groupInterleukin activity can beSoluble IL-8 receptor AIL-8Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful forreceptorB09989cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinproteinmonocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.hIL8RAfunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Holmes et aland lymphocytes), chemotaxis(1991) Science 253, 1278-80.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqJP08103276Interleukins are a groupInterleukin activity can beSoluble IL-8 receptorIL-8Accessionof multifunctionaldetermined using asays known inpolypeptides may be useful forreceptorB09990cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinproteinmonocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.hIL8Rfunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Holmes etand lymphocytes), chemotaxisal (1991) Science 253, 1278-80.of neutrophils and T lymphocytes,and/or inhibition of interferons.Inter-GeneSeqWO9621732-Interleukins are a groupInterleukin activity can beSoluble IL-2 receptorleukin-2Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful forreceptorR97569cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinassociatedmonocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.proteinfunctions include stimulatingPractical Approach, Clemens et al.,p43proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Gillis et aland lymphocytes), chemotaxis(1978) J. Immunol. 120, 2027.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqWO9629408Interleukins are a groupInterleukin activity can beSoluble IL-17 receptorinter-Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful forleukin-17W04185cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinreceptormonocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Yao et aland lymphocytes), chemotaxis(1995) J. Immunol. 155, 5483-86.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqWO9619574Interleukins are a groupInterleukin activity can beSoluble IL-11 receptorinter-Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful forleukin-11R99090cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinreceptormonocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Lu et aland lymphocytes), chemotaxis(1994) J immunol. Methods 173, 19.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqWO9623067Interleukins are a groupInterleukin activity can beInflammatory disorders,inter-Accessionof multifunctionaldetermined using assays known inimmunologicleukin-1W01911cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancerreceptormonocytes, and macrophages. KnownLymphokines and Interferons: Aaccessoryfunctions include stimulatingPractical Approach, Clemens et al.,proteinproliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Orencole &and lymphocytes), chemotaxisDinarello (1989) Cytokine 1, 14-of neutrophils and T lymphocytes,20.and/or inhibition of interferons.AGFGeneSeqUS5488032Interleukins are a groupInterleukin activity can beInflammatory disorders,ProteinAccessionof multifunctionaldetermined using assays known inimmunologicR92749cytokines synthesized by lymphocytes,the art: Matthews et al., indisorders, cancermonocytes, and macrophages. KnownLymphokines and Interferons: Afunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225.and lymphocytes), chemotaxisof neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqW09607739Interleukins are a groupInterleukin activity can beSoluble IL-type-3 receptorinter-Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful forleukin-1R91064cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukintype-3monocytes, and macrophages. KnownLymphokines and Interferons: Aactivitiesreceptorfunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Orencole &and lymphocytes), chemotaxisDinarello (1989) Cytokine 1, 14-of neutrophils and T lymphocytes,20.and/or inhibition of interferons.HumanGeneSeqWO9720926Interleukins are a groupInterleukin activity can beSoluble IL-13 beta receptorinter-Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful forleukin-13W24972cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinbetamonocytes, and macrophages. KnownLymphokines and Interferons Aactivities.receptorfunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Boutelier etand lymphocytes), chemotaxisal (1995) J. Immunol. Methodsof neutrophils and T lymphocytes,181, 29.and/or inhibition of interferons.HumanGeneSeqWO9720926Interleukins are a groupInterleukin activity can beSoluble IL-13 alpha receptorinter-Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful forleukin-13W24973cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinalphamonocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.receptorfunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Boutelier etand lymphocytes), chemotaxisal (1995) J. Immunol. Methodsof neutrophils and T lymphocytes,181, 29.and/or inhibition of interferons.HumanGeneSeqUS5599905Interleukins are a groupInterleukin activity can beSoluble IL-4 receptorinter-Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful forleukin-4W13499cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinreceptormonocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Siegel &and lymphocytes), chemotaxisMostowski (1990) J Immunolof neutrophils and T lymphocytes,Methods 132, 287-295.and/or inhibition of interferons.HumanGeneSeqEP759466Interleukins are a groupInterleukin activity can beSoluble IL-12 beta-2 receptorinter-Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful forleukin-12W12771cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinbeta-2monocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.receptorfunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Hori et aland lymphocytes), chemotaxis(1987), Blood 70, 1069-1078.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqEP759466Interleukins are a groupInterleukin activity can beSoluble IL-12 beta-1 receptorinter-Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful forleukin-12W12772cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinbeta-1monocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.receptor.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Hori et aland lymphocytes), chemotaxis(1987), Blood 70, 1069-1078.of neutrophils and T lymphocytes,and/or inhibition of interferons.Human IL-9GeneSeqWO9824904Interleukins are a groupInterleukin activity can beSoluble IL-9 receptorreceptorAccessionsof multifunctionaldetermined using assays known inpolypeptides may be useful forproteinW64055,cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinW64056,monocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.andfunctions include stimulatingPractical Approach, Clemens et al.,W64057proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Yang et aland lymphocytes), chemotaxis(1989), Blood 74, 1880-84..of neutrophils and T lymphocytes,and/or inhibition of interferons.IL-10GeneSeqUS5716804Interleukins are a groupInterleukin activity can beSoluble IL-10 receptorreceptorAccessionof multifunctionaldetermined using assays known inpolypeptides may be useful forW41804cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinmonocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Thompson-and lymphocytes), chemotaxisSnipes et al (1991) J. Exp. Med.of neutrophils and T lymphocytes,173, 507-510.and/or inhibition of interferons.Human IL-6GeneSeqJP11196867Interleukins are a groupInterleukin activity can beSoluble IL-6 receptorreceptorAccessionof multifunctionaldetermined using assays known inpolypeptides may be useful forY30938cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinmonocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Aarden etand lymphocytes), chemotaxisal(1987) Eur. J. Immunol 17,of neutrophils and T lymphocytes,1411-16.and/or inhibition of interferons.Il-17GeneSeqUS6096305Interleukins are a groupInterleukin activity can beSoluble IL-17 receptorreceptorAccessionof multifunctionaldetermined using assays known inpolypeptides may be useful forY97181cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinmonocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Yao et aland lymphocytes), chemotaxis(1995) J. Immunol. 155, 5483-86.of neutrophils and T lymphocytes,and/or inhibition of interferons.Il-17GeneSeqUS6100235Interleukins are a groupInterleukin activity can beSoluble IL-17 receptorreceptorAccessionof multifunctionaldetermined using assays known inpolypeptides may be useful forY97131cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinmonocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Yao et aland lymphocytes), chemotaxis(1995) J. Immunol. 155, 5483-86.of neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqEP509826Interleukins are a groupInterleukin activity can beSoluble IL-3 receptorinter-Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful forleukin-3R25300cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinreceptormonocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Kitamura etand lymphocytes), chemotaxisal (1989) J Cell Physiol. 140of neutrophils and T lymphocytes,323-334.and/or inhibition of interferons.HumanGeneSeqWO9102063Interleukins are a groupInterleukin activity can beSoluble GM-CSF receptorGM-CSFAccessionof multifunctionaldetermined using assays known inpolypeptides may be useful forreceptorR10919cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinmonocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225.and lymphocytes), chemotaxisof neutrophils and T lymphocytes,and/or inhibition of interferons.HumanGeneSeqEP492214Interleukins are a groupInterleukin activity can beSoluble IL-5 receptor alphaIL-5Accessionof multifunctionaldetermined using assays known inpolypeptides may be useful forreceptorR25064cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinalphamonocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.chainfunctions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Kitamura etand lymphocytes), chemotaxisal (1989) J Cell Physiol. 140,of neutrophils and T lymphocytes,323-334.and/or inhibition of interferons.Il-5GeneSeqWO9847923Interleukins are a groupInterleukin activity can beSoluble IL-5 receptorreceptorAccessionof multifunctionaldetermined using assays known inpolypeptides may be useful forW82842cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinmonocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Kitamura etand lymphocytes), chemotaxisal (1989) J Cell Physiol. 140,of neutrophils and T lymphocytes,323-334.and/or inhibition of interferons.Il-6GeneSeqJP05091892Interleukins are a groupInterleukin activity can beSoluble IL-6 receptorreceptorAccessionof multifunctionaldetermined using assays known inpolypeptides may be useful forR37215cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinmonocytes, and macrophages. KnownLymphokines and Interferons Aactivities.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Aarden etand lymphocytes), chemotaxisal (1987) Eur. J. Immunol 17,of neutrophils and T lymphocytes,1411-16.and/or inhibition of interferons.HumanGeneSeqAU8928720Interleukins are a groupInterleukin activity can beSoluble B cell stimulatingB cellAccessionof multifunctionaldetermined using assays known infactor-2 receptor polypeptidesstimu-P90525cytokines synthesized by lymphocytes,the art: Matthews et al., inmay be useful for inhibitinglatingmonocytes, and macrophages. KnownLymphokines and Interferons: Ainterleukin activities.factor-2functions include stimulatingPractical Approach, Clemens et al.,receptorproliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225.and lymphocytes), chemotaxisof neutrophils and T lymphocytes,and/or inhibition of interferons.IL-7GeneSeqEP403114Interleukins are a groupInterleukin activity can beSoluble IL-7 receptorreceptorAccessionof multifunctionaldetermined using assays known inpolypeptides may be useful forcloneR08330cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinmonocytes, and macrophages. KnownLymphokines and Interferons. Aactivities.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Park et aland lymphocytes), chemotaxis(1990) J. Exp. Med. 171,of neutrophils and T lymphocytes,1073-79.and/or inhibition of interferons.EPOGeneSeqWO9008822EPO Receptor is involved in theEPO Receptor activity can beInflammatory disorders,receptor;Accessionproliferation and differentiation ofdetermined using assays known inimmunologic disorders,EPORR06512erythroblasts.the art, such as, J Biol Chem 2001cancer, erythroblastMar. 23; 276(12:8995-9002; JAK2proliferation andprotein tyrosine kinase activity:differentiationBlood 1994 Sep. 1; 84(5):1501-7and Mol Cell Biol. 1994Oct.; 14(10:6506-14.IL-15GeneSeqWO9530695Interleukins are a groupInterleukin activity can beSoluble IL-15 receptorreceptorAccessionof multifunctionaldetermined using assays known inpolypeptides may be useful forR90843cytokines synthesized by lymphocytes,the art: Matthews et al., ininhibiting interleukinmonocytes, and macrophages. KnownLymphokines and Interferons: Aactivities.functions include stimulatingPractical Approach, Clemens et al.,proliferation of immune cells (e.g.,eds, IRL Press, Washington, D.C.T helper cells, B cells, eosinophils,1987, pp. 221-225; and Giri et aland lymphocytes), chemotaxis(1994) EMBO J. 13 2822-2830.of neutrophils and T lymphocytes,and/or inhibition of interferons.CD137;GeneSeqWO9507984Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBSoluble 4-1BB receptor4-1BBAccessionactivation, and co-stimulation of immuneactivation, and B and T cell co-polypeptides may be useful forReceptorR70977cells such as T and B cells.stimulation can be determinedinhibiting apoptosis, NF-kBProteinusing assays known in the art:activation, and/or co-Moore et al, 1999, Science, 285(5425):stimulation of immune cells260-3; Song H Y et al., 1997such as B and T cells.Proc Natl Acad Sci USA94(18):9792-6; Epsevik andNissen-Meyer, 1986, J. Immunol.Methods.BCMAGeneSeqWO0068378Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBSoluble BCMA receptorAccessionactivation, and co-stimulation of immuneactivation, and B and T cell co-polypeptides may be useful forY71979cells such as T and B cells.stimulation can be determinedinhibiting apoptosis, NF-kBusing assays known in the art:activation, and/or co-Moore et al., 1999, Science, 285(5425):stimulation of immune cells260-3; Song H Y et al.,such as B and T cells.1997 Proc Natl Acad Sci USA94(18):9792-6; Epsevik andNissen-Meyer, 1986, J. Immunol.Methods.CD27GeneSeqWO9201049Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBSoluble CD27 polypeptidesAccessionactivation, and co-stimulation of immuneactivation, and B and T cell co-may be useful for inhibitingR20814cells such as T and B cells.stimulation can be determinedapoptosis, NF-kB activation,using assays known in the art:and/or co-stimulation ofMoore et al., 1999, Science, 285(5425):immune cells such as B and T260-3; Song H Y et al.,cells.1997 Proc Natl Acad Sci USA94(18):9792-6; Epsevik andNissen-Meyer, 1986, J. Immunol.Methods.CD30GeneSeqDE4200043Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBSoluble CD30 polypeptidesAccessionactivation, and co-stimulation of immuneactivation, and B and T cell co-may be useful for inhibitingR35478cells such as T and B cells.stimulation can be determinedapoptosis, NF-kB activation,using assays known in the art:and/or co-stimulation ofMoore et al., 1999, Science, 285(5425):immune cells such as B and T260-3; Song H Y et al.,cells.1997 Proc Natl Acad Sci USA94(18):9792-6; Epsevik andNissen-Meyer, 1986, J. Immunol.Methods.CD40GeneSeqWO9945944Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBSoluble CD40 polypeptidesAccessionactivation, and co-stimulation of immuneactivation, and B and T cellmay be useful for inhibitingY33499cells such as T and B cells.co-stimulation can be determinedapoptosis, NF-kB activation,using assays known in the art:and/or co-stimulation ofMoore et al., 1999, Scienceimmune cells such as B and T285(5425):260-3; Song H Y et al.,cells.1997 Proc Natl Acad Sci USA94(18):9792-6; Epsevik andNissen-Meyer, 1986, J. Immunol.Methods.EDARGenbankActivities associated with apoptosis, NF-kBApoptosis activity, NF-kBImmune Disorders, Lymphomas,Accessionactivation, and co-stimulation of immuneactivation, and B and T cell co-X-linkedAAD50077cells such as T and B cells.stimulation can be determined usinghypohidrotic ectodermalassays known in the art: Moore etdysplasiaal, 1999, Science, 285(5425):260-3;Song H Y et al., 1997 Proc NatlAcad Sci USA 94(18):9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.OX40;GeneSeqWO9512673Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBImmune Disorders, Lymphomas,ACT-4Accessionactivation, and co-stimulation of immuneactivation, and B and T cell co-T cell disordersR74737cells such as T and B cells.stimulation can be determined usingassays known in the art: Moore etal., 1999, Science, 285(5425):260-3;Song H Y et al., 1997 Proc NatlAcad Sci USA 94(18):9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.TACIGeneSeqWO9839361Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBSoluble TACI receptorAccessionactivation, and co-stimulation of immuneactivation, and B and T cell co-polypeptides may be useful forW75783cells such as T and B cells.stimulation can be determined usinginhibiting apoptosis, NF-kBassays known in the art: Moore etactivation, and/or co-al., 1999, Science, 285(5425):260-3;stimulation of immune cellsSong H Y et al., 1997 Proc Natlsuch as B and T cells.Acad Sci USA 94(18):9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.TNF-RGeneSeqAU9058976Activities associates with apoptosis, NF-kBApoptosis activity, NF-kBSoluble TNF-R receptorAccessionactivation, and co-stimulation of immuneactivation, and B and T cell co-polypeptides may be useful forR10986cells such as T and B cells.stimulation can be determined usinginhibiting apoptosis, NF-kBassays known in the art: Moore etactivation, and/or co-al., 1999, Science, 285(5425):260-3;stimulation of immune cellsSong H Y et al., 1997 Proc Natlsuch as B and T cells.Acad Sci USA 94(18):9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.TNF-RII;GeneSeqEP418014Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBSoluble TNFR-II receptorTNFAccessionactivation, and co-stimulation of immuneactivation, and B and T cell co-polypeptides may be useful forp75R11141cells such as T and B cells.stimulation can be determined usinginhibiting apoptosis, NF-kBreceptor;assays known in the art: Moore etactivation, and/or co-Deathal., 1999, Science, 285(5425):260-3;stimulation of immune cellsReceptorSong H Y et al., 1997 Proc Natlsuch as B and T cells.Acad Sci USA 94(18)9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.hAPO-4;GeneSeqWO9911791Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBImmune Disorders, CancersTROYAccessionactivation, and co-stimulation of immuneactivation, and B and T cell co-W93581cells such as T and B cells.stimulation can be determined usingassays known in the art: Moore etal., 1999, Science, 285(5425):260-3;Song H Y et al., 1997 Proc NatlAcad Sci USA 94(18):9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.TNF-alphaGeneSeqEP205038Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBInflammatory disorders,precursorAccessionactivation, and co-stimulation of immuneactivation, and B and T cell co-immunologicP60074cells such as T and B cells.stimulation can be determined usingdisorders, cancerassays known in the art: Moore etal., 1999, Science, 285(5425):260-3;Song H Y et al., 1997 Proc NatlAcad Sci USA 94(18):9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.HumanGeneSeqEP619372Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBInflammatory disorders,TNF-alphaAccessionactivation, and co-stimulation of immuneactivation, and B and T cell co-immunologicR62463cells such as T and B cellsstimulation can be determined usingdisorders, cancerassays known in the art: Moore etal., 1999, Science, 285(5425):260-3;Song H Y et al., 1997 Proc NatlAcad Sci USA 94(18):9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.HumanGeneSeqEP563714Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBInflammatory disorders,TNF-alphaAccessionactivation, and co-stimulation of immuneactivation, and B and T cell co-immunologicR42679cells such as T and B cells.stimulation can be determined usingdisorders, cancerassays known in the art: Moore etal, 1999, Science, 285(5425):260-3;Song H Y et al., 1997 Proc NatlAcad Sci USA 94(18):9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.HumanGeneSeqWO0064479Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBInflammatory disorders,TNF-betaAccessionactivation, and co-stimulation of immuneactivation, and B and T cell co-immunologic(LT-alpha)B37799cells such as T and B cells.stimulation can be determined usingdisorders, cancerassays known in the art: Moore etal., 1999, Science, 285(5425):260-3;Song H Y et al., 1997 Proc NatlAcad Sci USA 94(18):9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.LT-alphaGeneSeqEP250000Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBInflammatory disorders,Accessionactivation, and co-stimulation of immuneactivation, and B and T cell co-immunologicP70107cells such as T and B cells.stimulation can be determined usingdisorders, cancerassays known in the art: Moore etal., 1999, Science, 285(5425):260-3;Song H Y et al., 1997 Proc NatlAcad Sci USA 94(18):9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.LT-betaGeneSeqWO9413808Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBInflammatory disorders,Accessionactivation, and co-stimulation of immuneactivation, and B and T cell co-immunologicR56869cells such as T and B cells.stimulation can be determined usingdisorders, cancerassays known in the art: Moore etal, 1999, Science, 285(5425):260-3;Song H Y et al., 1997 Proc NatlAcad Sci USA 94(18)9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.OPGLGeneSeqWO9846751Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBInflammatory disorders,Accessionactivation, and co-stimulation of immuneactivation, and B and T cell co-immunologicW83195cells such as T and B cells.stimulation can be determined usingdisorders, cancer,assays known in the art: Moore etloss of bone massal., 1999, Science, 285(5425):260-3;Song H Y et al., 1997 Proc NatlAcad Sci USA 94(18)9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.FasLGeneSeqWO9903999Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBInflammatory disorders,Accessionactivation, and co-stimulation of immuneactivation, and B and T cell co-immunologicW98071cells such as T and B cells.stimulation can be determined usingdisorders, cancerassays known in the art: Moore, etal., 1999, Science, 285(5425):260-3;Song H Y et al., 1997 Proc NatlAcad Sci USA 94(18)9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.FasLGeneSeqWO9903998Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBInflammatory disorders,Accessionactivation, and co-stimulation of immuneactivation, and B and T cell co-immunologicW95041cells such as T and B cells.stimulation can be determined usingdisorders, cancerassays known in the art: Moore etal., 1999, Science, 285(5425):260-3;Song H Y et al., 1997 Proc NatlAcad Sci USA 94(18):9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.CD27LGeneSeqWO9405691Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBInflammatory disorders,Accessionactivation, and co-stimulation of immuneactivation, and B and T cell co-immunologicR50121cells such as T and B cells.stimulation can be determined usingdisorders, cancerassays known in the art: Moore etal., 1999, Science, 285(5425):260-3;Song H Y et al., 1997 Proc NatlAcad Sci USA 94(18):9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.CD30GeneSeqWO9324135Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBInflammatory disorders,ligandAccessionactivation, and co-stimulation of immuneactivation, and B and T cell co-immunologicR45007cells such as T and B cells.stimulation can be determined usingdisorders, cancerassays known in the art: Moore etal., 1999, Science, 285(5425):260-3;Song H Y et al., 1997 Proc NatlAcad Sci USA 94(18):9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.CD40LGeneSeqWO9529935Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBInflammatory disorders,Accessionactivation, and co-stimulation of immuneactivation, and B and T cell co-immunologicR85486cells such as T and B cells.stimulation can be determined usingdisorders, cancerassays known in the art: Moore, etal., 1999, Science, 285(5425):260-3;Song H Y et al., 1997 Proc NatlAcad Sci USA 94(18):9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.4-1BBGeneSeqUS5674704Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBInflammatory disorders,ligandAccessionactivation, and co-stimulation of immuneactivation, and B and T cell co-immunologicW26657cells such as T and B cells.stimulation can be determined usingdisorders, cancerassays known in the art: Moore etal., 1999, Science, 285(5425):260-3;Song H Y et al, 1997 Proc NatlAcad Sci USA 94(18):9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.FASGeneSeqWO0058465Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBSoluble DcR3 polypeptidesLigandAccessionactivation, and co-stimulation of immuneactivation, and B and T cell co-may be useful for inhibitingInhibitoryB19335cells such as T and B cells.stimulation can be determined usingapoptosis, NF-kB activation,Proteinassays known in the art: Moore etand/or co-stimulation of(DcR3)al., 1999, Science, 285(5425):260-3;immune cells such as B and TSong H Y et al., 1997 Proc Natlcells.Acad Sci USA 94(18):9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. MethodsOX40LGeneSeqWO9521915Activities associated with apoptosis, NF-kBApoptosis activity, NF-kBInflammatory disorders,Accessionactivation, and co-stimulation of immuneactivation, and B and T cell co-immunologicR79903cells such as T and B cells.stimulation can be determined usingdisorders, cancerassays known in the art: Moore etal, 1999, Science, 285(5425):260-3;Song H Y et al., 1997 Proc NatlAcad Sci USA 94(18):9792-6;Epsevik and Nissen-Meyer, 1986, J.Immunol. Methods.ProteaseGeneSeqWO9106561Peptides that inhibit theHIV protease activities are known inHIV, inflammatory disorders,inhibitorAccessionsfunction/binding of HIVthe art: HIV protease assays:immunologic disorders,peptidesR12435,EP0387231. One can modify thecancer, viral infectionsR12436,assay to look for inhibition usingR12437,any of the disclosed proteaseR12438,inhibitor polypeptides.R12439,R12440,andR1244RetroGeneSeqEP387231Peptides that inhibit theHIV protease activities are known inHIV, inflammatory disorders,viralAccessionsfunction/binding of HIVthe art: HIV protease assays:immunologic disorders,proteaseR06660,EP0387231. One can modify thecancer, viral infectionsinhibitorsR06661,assay to look for inhibition usingR06662,any of the disclosed proteaseR06663,inhibitor polypeptides.R06664,R06665,R06666,R06667,R06668,R06669,R06670,R06671,R06672,R06673,R06674,R06675,andR06676HIVGeneSeqWO9301828Peptides that inhibit theHIV protease activities are known in theHIV, inflammatory disorders,proteaseAccessionsfunction/binding of HIVart. HIV protease assays EP0387231.immunologic disorders,inhibitingR59293,One can modify the assay to look forcancer, viral infectionspeptidesR59294,inhibition using any of the disclosedR59295,protease inhibitor polypeptidesR59296,R59297,R59298,R59299,R592300,R59301,R59302,R59301,R59302,R59303,R59304,R59305,R59306,R59307,R59308,R59309,R59310,R59311,R59312,R59313,R59314,R59315,R59316,R59317R59318,R59319,R59320,R59321,R59322,R59323,R59324,R59325,R59326,R59327,R59328,R59329,R59330,R59331,R59332,R59333,R59334,R59335,R59336,R59337,R59338,R59339,R59340,R59341,R59342,R59343,R59344,R59345,R59346,R59347,R59348,R59349,andR59350HIV-1GeneSeqDE4412174Peptides that inhibit theHIV protease activities are known in theHIV, inflammatory disorders,proteaseAccessionsfunction/binding of HIVart: HIV protease assays: EP0387231.immunologic disorders,hinibitorsR86326,One can modify the assay to look forcancer, viral infectionsR86327,inhibition using any of the disclosedR86328,protease inhibitor polypeptidesR86329,R86330,R86331,R86332,R86333,R86334,R86335,R86336,R86337,R86338,R86339,R86340,R86341,R86342,R86343,R86344,R86345,R86346,R86347,R86348,R86349,R86350,R86351,R86352,R86353,R86354,R86355,R86356,R86357,R86358,R86359,R86360,R86361,R86362,R86363,R86364,R86365,R86366,R86367,R86368,R86369,R86370,andR86371HIVGeneSeqWO9959615Peptides that inhibit theHIV protease activities are known inHIV, inflammatory disorders,InhibitorAccessionfunction/binding of HIVthe art: HIV protease assays:immunologic disorders,PeptideY89687EP0387231. One can modify thecancer, viral infectionsassay to look for inhibition usingany of the disclosed proteaseinhibitor polypeptides.HIVGenSeqWO9948513Peptides that inhibit theHIV Protease activities are knownHIV, inflammatory disorders,InhibitorAccessionfunction/binding of HIVin the art; HIV protease assays:immunologic disorders,PeptideY31955EP0387231. One can modify thecancer, viral infectionsassay to look for inhibition usingany of the disclosed proteaseinhibitor polypeptides.HIVwww.sciencexPeptides that inhibit theHIV protease activities are knownHIV, inflammatory disorders,Inhibitorpress.org;function/binding of HIVin the art: HIV protease assays:immunologic disorders,PeptidePublishedEP0387231. One can modify thecancer, viral infectionsonline 12assay to look for inhibition usingJan. 2001;any of the disclosed protease10.1126/sciencinhibitor polypeptides.e.1057453HumanGeneSeqWO9509232Chemokines are a familyChemokine activities can beImmune disorders, particularlymonocyteAccessionof related small, secreted proteinsdetermined using assays known inuseful for treating bacterialchemoR73915involved in biological processesthe art: Methods in Molecularand/or viral menigitisattractantranging from hematopoiesis,Biology, 2000, vol. 138:factorangiogenesis, and leukocyte trafficking.Chemokine Protocols, Edited by:hMCP-3Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9509232Chemokines are a familyChemokine activities can beImmune disorders, particularlymonocyteAccessionof related small, secreted proteinsdetermined using assays known inuseful for treating bacterialchemo-R73914involved in biological processesthe art: Methods in Molecularand/or viral menigitisattractantranging from hematopoiesis,Biology, 2000, vol. 138:factorangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:hMCP-1Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9429341Chemokines are a familyChemokine activities can beImmune disorders,gro-betaAccessionsof related small, secreted proteinsdetermined using assays known ininflammatory disorders,chemo-R66699involved in biological processesthe art: Methods in Molecularblood-related disorders,kineandranging from hematopoiesis,Biology, 2000, vol. 138:stem cell transplantation,W17671angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:cancerMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9429341Chemokines are a familyChemokine activities can beImmune disorders,gro-Accessionsof related small, secreted proteinsdetermined using assays known ininflammatory disorders,gammaR66700involved in biological processesthe art: Methods in Molecularblood-related disorders,chemokineandranging from hematopoiesis,Biology, 2000, vol. 138:stem cell transplantation,W17672angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:cancerMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9429341Chemokines are a familyChemokine activities can beImmune disorders,gro-alphaAccessionsof related small, secreted proteinsdetermined using assays known ininflammatory disorders,chemokineR66698 andinvolved in biological processesthe art: Methods in Molecularblood-related disorders,W18024ranging from hematopoiesis,Biology, 2000, vol. 138:stem cell transplantation,angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:cancerMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9632481Chemokines are a familyChemokine activities can beImmune disorders, particularlyeosinophil-Accessionof related small, secreted proteinsdetermined using assays known intreatment of eosinophilia,expressedW05186involved in biological processesthe art: Methods in Molecularinflammation,chemokineranging from hematopoiesis,Biology, 2000, vol. 138:allergies, asthma, leukaemia(EEC)angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:and lymphomaMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.Chemo-GeneSeqWO9613587Chemokines are a familyChemokine activities can beCancer and blood-relatedkine-likeAccessionsof related small, secreted proteinsdetermined using assasys known indisorders, particularlyproteinR92318involved in biological processesthe art: Methods in MolecularmyelosuppressionPF4-414andranging from hematopoiesis,Biology, 2000, vol. 138:Full-R99809angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:LengthMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,andsimilarly diverse range of pathologiesand C. A. Power. © Humana PressMatureincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.Chemo-GeneSeqWO9613587Chemokines are a familyChemokine activities can beCancer and blood-relatedkine-likeAccessionof related small, secreted proteinsdetermined using assasys known indisorders, particularlyproteinR99812involved in biological processesthe art: Methods in MolecularmyelosuppressionIL-8M3ranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ; and Holmes et alrejection, viral infection, and tumor(1991) Science 253, 1278-80.biology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9613587Chemokines are a familyChemokine activities can beCancer and blood-relatedinter-Accessionof related small, secreted proteinsdetermined using assasys known indisorders, particularlyleukin-8R99814involved in biological processesthe art: Methods in Molecularmyelosuppression(IL-8)ranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJ; and Holmes et alrejection, viral infection, and tumor(1991) Science 253, 1278-80.biology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.Chemo-GeneSeqWO9613587Chemokines are a familyChemokine activities can beCancer and blood-relatedkine-likeAccessionsof related small, secreted proteinsdetermined using assasys known indisorders, particularlyproteinR99815 andinvolved in biological processesthe art: Methods in MolecularmyelosuppressionIL-8M1R99803ranging from hematopoiesis,Biology, 2000, vol. 138:Full-angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:LengthMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,andsimilarly diverse range of pathologiesand C. A. Power. © Humana PressMatureincluding inflammation, allergy, tissueInc., Totowa, NJ; and Holmes et alrejection, viral infection, and tumor(1991) Science 253, 1278-80.biology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.Chemo-GeneSeqWO9613587Chemokines are a familyChemokine activities can beCancer and blood-relatedkine-likeAccessionsof related small, secreted proteinsdetermined using assays known indisorders, particularlyproteinR99816involved in biological processesthe art: Methods in Molecularmyelosuppression.IL - 8M8andranging from hematopoiesis,Biology, 2000, vol. 138:Full-R99805angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:LengthMembers of this family are involved in aA. E. I. Proudfoot; T. N. C. Wells,andsimilarly diverse range of pathologiesand C. A. Power. © Humana PressMatureincluding inflammation, allergy, tissueInc., Totowa, NJ; and Holmes et alrejection, viral infection, and tumor(1991) Science 253, 1278-80.biology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.Chemo-GeneSeqWO9613587Chemokines are a familyChemokine activities can beCancer and blood-relatedkine-likeAccessionsof related small, secreted proteinsdetermined using assays known indisorders, particularlyproteinR99817involved in biological processesthe art: Methods in Molecularmyelosuppression.IL - 8M8andranging from hematopoiesis,Biology, 2000, vol. 138: ChemokineFull-R99806angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.LengthMembers of this family are involved in aProudfoot; T. N. C. Wells, and C. A.andsimilarly diverse range of pathologiesPower. © Humana Press Inc.,Matureincluding inflammation, allergy, tissueTotowa, NJ; and Holmes et alrejection, viral infection, and tumor(1991) Science 253, 1278-80.biology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.Chemo-GeneSeqWO9613587Chemokines are a familyChemokine activities can beCancer and blood-relatedkine-likeAccessionsof related small, secreted proteinsdetermined using assays known indisorders, particularlyproteinR99818involved in biological processesthe art: Methods in Molecularmyelosuppression.IL - 8M8andranging from hematopoiesis,Biology, 2000, vol. 138: ChemokineFull-R99804angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.LengthMembers of this family are involved in aProudfoot; T. N. C. Wells, and C. A.andsimilarly diverse range of pathologiesPower. © Humana Press Inc.,Matureincluding inflammation, allergy, tissueTotowa, NJ; and Holmes et alrejection, viral infection, and tumor(1991) Science 253, 1278-80.biology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.Chemo-GeneSeqWO9613587Chemokines are a familyChemokine activities can beCancer and blood-relatedkine-likeAccessionsof related small, secreted proteinsdetermined using assays known indisorders, particularlyproteinR99819involved in biological processesthe art: Methods in Molecularmyelosuppression.IL - 8M8andranging from hematopoiesis,Biology, 2000, vol. 138: ChemokineFull-R99807angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.LengthMembers of this family are involved in aProudfoot; T. N. C. Wells, and C. A.andsimilarly diverse range of pathologiesPower. © Humana PressMatureincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.Chemo-GeneSeqWO9613587Chemokines are a familyChemokine activities can beCancer and blood-relatedkine-likeAccessionsof related small, secreted proteinsdetermined using assays known indisorders, particularlyproteinR99822 andinvolved in biological processesthe art: Methods in Molecularmyelosuppression.IL - 8M8R9807ranging from hematopoiesis,Biology, 2000, vol. 138: ChemokineFull-angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.LengthMembers of this family are involved in aProudfoot; T. N. C. Wells, and C. A.andsimilarly diverse range of pathologiesPower. © Humana PressMatureincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9622374Chemokines are a familyChemokine activities can beImmune disordersfoetalAccessionof related small, secreted proteinsdetermined using assasys known inspleenR98499involved in biological processesthe art: Methods in Molecularexpressedranging from hematopoiesis,Biology, 2000, vol. 138: Chemokinechemo-angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.kine,Members of this family are involved in aProudfoot; T. N. C. Wells, and C. A.FSECsimilarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.LiverGeneSeqWO9616979Chemokines are a familyChemokine activities can beInflammation of the liverexpressedAccessionof related small, secreted proteinsdetermined using assasys known inchemo-R95689involved in biological processesthe art: Methods in Molecularkine-1ranging from hematopoiesis,Biology, 2000, vol. 138: Chemokine(LVEC-1)angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot; T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.LiverGeneSeqWO9616979Chemokines are a familyChemokine activities can beInflammation of the liverexpressedAccessionof related small, secreted proteinsdetermined using assasys known inchemokine-2R95690involved in biological processesthe art: Methods in Molecular(LVEC-2)ranging from hematopoiesis,Biology, 2000, vol. 138: Chemokineangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot; T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.PituitaryGeneSeqWO9616979Chemokines are a familyChemokine activities can beInflammation, particularly ofexpressedAccessionof related small, secreted proteinsdetermined using assays known inthe liverchemokineR95691involved in biological processesthe art: Methods in Molecular(PGEC)ranging from hematopoiesis,Biology, 2000, vol. 138: Chemokineangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot; T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.Adenoid-GeneSeqWO9617868Chemokines are a familyChemokine activities can beInflammation, angiogenesis,expressedAccessionof related small, secreted proteinsdetermined using assays known intumorigenesis, musculoskeletalchemokineR97664involved in biological processesthe art: Methods in Moleculardisorders(ADEC)ranging from hematopoiesis,Biology, 2000, vol. 138: Chemokineangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot; T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9741230Chemokines are a familyChemokine activities can beImmune disorders, cellchemo-Accessionof related small, secreted proteinsdetermined using assays known inmigration, proliferation, andkineW38170involved in biological processesthe art: Methods in Moleculardifferentiation disordersCC-2ranging from hematopoiesis,Biology, 2000, vol. 138;angiogenesis, and leukocyte trafficking.Chemokine protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc. Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9741230Chemokines are a familyChemokine activities can beImmune disorders, cellchemokineAccessionof related small, secreted proteinsdetermined using assays known inmigration, proliferation, andHCC-1W38171involved in biological processesthe art: Methods in moleculardifferentiation disordersranging from hematopoiesis,Biology 2000, vol. 138: Chemokineangiogenesis, and leukocyte trafficking.Protocols. Edited by A. E. I.Members of this family are involved in aProudfoot, T. N. C. Wells and C. A.similarly diverse range of pathologiesPower © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9741230Chemokines are a familyChemokine activities can beImmune disorders, cellchemokineAccessionof related small, secreted proteinsdetermined using assays known inmigration, proliferation andCC-3W38172involved in biological processesthe art: Methods in moleculardifferentiation disordersranging from hematopiesis,Biology, 2000, vol. 138:anglogenesis, and leukocyte trafficking.Chemokine Protocols, Edited byMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells, andsimilarly diverse range of pathologiesC. A. Power © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.NovelGeneSeqWO9739126Chemokines are a familyChemokine activities can beImmune disorders, vascularbeta-Accessionof related small, secreted proteinsdetermined using assays known indisorders, cancerchemokineW27271involved in biological processesthe art: Methods in moleculardesignatedranging from hematopoiesis,Biology, 2000, vol. 138:PTECanglogenesis, and leukocyte trafficking.Chemokine Protocols, Edited byMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells, andsimilarly diverse range of pathologiesC. A. Power © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9727299Chemokines are a familyChemokine activities can beImmune disorders, inflammatoryCX3CAccessionof related small, secreted proteinsdetermined using assays known indiseases, abnormal111 aminoW23344involved in biological processesthe art: Methods in molecularproliferation,acidranging from hematopoiesis,Biology, 2000, vol. 138:regeneration, degeneration,chemokineanglogenesis, and leukocyte trafficking.Chemokine Protocols, Edited byand atrophyMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells, andsimilarly diverse range of pathologiesC. A. Power © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9721812Chemokines are a familyChemokine activities can beAbnormal physiology andCCF18Accessionof related small, secreted proteinsdetermined using assays known indevelopment disorders, canchemokineW25942involved in biological processesthe art: Methods in molecularalso be used as an anti-viralranging from hematopoiesis,Biology, 2000, vol. 138:agentanglogenesis, and leukocyte trafficking.Chemokine Protocols, Edited byMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells, andsimilarly diverse range of pathologiesC. A. Power © Humanaincluding inflammation, allergy, tissuePress Inc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9725427Chemokines are a familyChemokine activities can beChemotaxis, blood-relatedbeta-Accessionof related small, secreted proteinsdetermined using assays known indisorders, viral infection,chemokineW26655involved in biological processesthe art: Methods in molecularHIV, wound healing, cancerH1305ranging from hematopoiesis,Biology, 2000, vol. 138:(MCP-2)anglogenesis, and leukocyte trafficking.Chemokine Protocols, Edited byMembers of this family are involved in aA. E. I. Proudfoot, T. N. C.similarly diverse range of pathologiesWells, and C. A. Power ©including inflammation, allergy, tissueHumana Press Inc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9712914Chemokines are a familyChemokine activities can beInflammatory and immuneeosino-Accessionof related small, secreted proteinsdetermined using assays known indisorderscyte CCW14990involved in biological processesthe art: Methods in moleculartyperanging from hematopoiesis,Biology, 2000, vol. 138:chemokineanglogenesis, and leukocyte trafficking.Chemokine Protocols, Edited byeotaxinMembers of this family are involved in aA. E. I. Proudfoot, T. N. C.similarly diverse range of pathologiesWells, and C. A. Power ©including inflammation, allergy, tissueHumana Press Inc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9711969Chemokines are a familyChemokine activities can beInflammatory and immunethymusAccessionof related small, secreted proteinsdetermined using assays known indisordersandW14018involved in biological processesthe art: Methods in molecularactivationranging from hematopoiesis,Biology, 2000, vol. 138:regulatedanglogenesis, and leukocyte trafficking.Chemokine Protocols, Edited bycytokineMembers of this family are involved in aA. E. I. Proudfoot, T. N. C.(TARC)similarly diverse range of pathologiesWells, and C. A. Power ©including inflammation, allergy, tissueHumana Press Inc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9712041Chemokines are a familyChemokine activities can beCancer, wound healing,chemokineAccessionof related small, secreted proteinsdetermined using assays known inimmune disordersbeta-W16315involved in biological processesthe art: Methods in molecular8 shortranging from hematopoiesis,Biology, 2000, vol. 138:formsanglogenesis, and leukocyte trafficking.Chemokine Protocols, Edited byMembers of this family are involved in aA. E. I. Proudfoot, T. N. C.similarly diverse range of pathologiesWells, and C. A. Power ©including inflammation, allergy, tissueHumana Press Inc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.MicrophageGeneSeqWO9640923Chemokines are a familyChemokine activities can beInflammatory diseases, woundderivedAccessionof related small, secreted proteinsdetermined using assays known inhealin, angiogenesischemokine,W20058involved in biological processesthe art: Methods in MolecularMDCranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C.similarly diverse range of pathologiesWells, and C. A. Power ©including inflammation, allergy, tissueHumana Press Inc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9844117Chemokines are a familyChemokine activities can beInflammatory andchemokineAccessionof related small, secreted proteinsdetermined using assays known inimmune diseasesZSIG-35W30565involved in biological processesthe art: Methods in Molecularranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. ©Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.PrimateGeneSeqWO98328658Chemokines are a family of relatedChemokine activities can beImmune andCCAccesssionsmall, secreted proteins involved indetermined using assays known ininflammatorychemokineW69990biological processes ranging fromthe art: Methods in Moleculardisorders, abnormal“ILINCK”hematopoiesis, angiogenesis, andBiology, 2000, vol. 138:proliferation, regeneration,leukocyte trafficking.Chemokine Protocols. Edited by:generationA. E. I. Proudfoot, T. N. C.and atrophy disordersWells, and C. A. Power ©Humana Press Inc., Totowa, NJPrimateGeneSeqWO9832858Chemokines are a familyChemokine activities can beImmune and inflammatoryCXCAccessionof related small, secreted proteinsdetermined using assays known indisorders, abnormalchemokineW69989involved in biological processesthe art: Methods in Molecularproliferation,“IBICK”ranging from hematopoiesis,Biology, 2000, vol. 138:regeneration, generation andangiogenesis, and leukocyte trafficking.Chemokine Protocols. Editd by.atrophy disordersMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. ©Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9831809Chemokines are a familyChemokine activities can beImmune, inflammatory, andCC-typeAccessionof related small, secreted proteinsdetermined using assays known ininfectious disorders, cancerchemokineW69163involved in biological processesthe art: Methods in Molecularproteinranging from hematopoiesis,Biology, 2000, vol. 138:designatedangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:SLCMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,(secondarysimilarly diverse range of pathologiesand C. A. Power. ©Humana Presslymphoidincluding inflammation, allergy, tissueInc., Totowa, NJchemokine)rejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9826071Chemokines are a familyChemokine activities can beCancer and infectiousCCAccessionof related small, secreted proteinsdetermined using assays known indiseases, particularlychemokineW62542involved in biological processesthe art: Methods in Molecularherpes virusELCranging from hematopoiesis,Biology, 2000, vol. 138:proteinangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWo9823750Chemokines are a familyChemokine activities can beAbnormal proliferation,DVic-1Accessionof related small, secreted proteinsdetermined using assays known inregeneration, degeneration,C-CW60649involved in biological processesthe art: Methods in Molecularand atrophy disorders,chemokineranging from hematopoiesis,Biology, 2000, vol. 138:including cancerangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9823750Chemokines are a familyChemokine activities can beImmune disorders, cellC-CAccessionof related small, secreted proteinsdetermined using assays known inproliferation disorders, cancerchemokineW60650involved in biological processesthe art: Methods in MolecularDGWCCranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9824907Chemokines are a familyChemokine activities can beImmune disorders,STCP-1Accessionof related small, secreted proteinsdetermined using assays known inparticularly T cellW62783involved in biological processesthe art: Methods in Molecularrelated disorders, viralranging from hematopoiesis,Biology, 2000, vol. 138:infection, andangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:inflammation, especially jointMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.ExoduaGeneSeqWO9821330Chemokines are a familyChemokine activities can beImmune and inflammatoryproteinAccessionof related small, secreted proteinsdetermined using assays known indisorders, angiogenesis, cancer,W61279involved in biological processesthe art: Methods in Molecularand proliferationranging from hematopoiesis,Biology, 2000, vol.138: Chemokinedisorders, particularlyangiogenesis, and leukocyte trafficking.Protocols. Edited by. A. E. I.myeloproliferativeMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.diseasessimilarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9814581Chemokines are a familyChemokine activities can beCancer and degenerativeChemokineAcessionof related small, secreted proteinsdetermined using assays known indisordersproteinW50887involved in biological processesthe art: Methods in Molecularranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols, Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqUS5780268Chemokines are a familyChemokine activities can beImmune, inflammatory, andT cellAccessionof related small, secreted proteinsdetermined using assays known ininfectious disorders, cancermixedW58703involved in biological processesthe art: Mehtods of Molecularlymphocyteranging from hematopoiesis,Biology, 2000, vol. 138:reactionangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:expressedMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,chemokinesimilarly diverse range of pathologiesand C. A. Power © Humana Press(TMEC)including inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqW09814581Chemokines are a familyChemokine activities can beCancer and degenerative6CKineAccessionof related small, secreted proteinsdetermined using assays known indisordersproteinW50885involved in biological processesthe art: Mehtods of Molecularranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.humanGeneSeqWO9817800Chemokines are a familyChemokine activities can beImmune, inflammatory, andliverAccessionof related small, secreted proteinsdetermined using assays known ininfectious disorders, cancerandW57475involved in biological processesthe art: Mehtods of Molecularactivationranging from hematopoiesis,Biology, 2000, vol. 138:regulatedangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:chemokineMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,(LARC)similarly diverse range of pathologiesand C. A. Power © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.RANTESGeneSeqWO9744462Chemokines are a familyChemokine activities can beInfectious diseases,peptideAccessionof related small, secreted proteinsdetermined using assays known inparticularly HIVW29538involved in biological processesthe art: Mehtods of Molecularranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.RANTESGeneSeqWO9744462Chemokines are a familyChemokine activities can beInfectious diseases,8-68Accessionof related small, secreted proteinsdetermined using assays known inparticularly HIVW29529involved in biological processesthe art: Mehtods of Molecularranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.RANTESGeneSeqWO9744462Chemokines are a familyChemokine activities can beInfectious diseases,9-68Accessionof related small, secreted proteinsdetermined using assays known inparticularly HIVW29528involved in biological processesthe art: Mehtods of Molecularranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9811226Chemokines are a familyChemokine activities can beAbnormal proliferation,chemokineAccessionof related small, secreted proteinsdetermined using assays known inregeneration,proteinW59433involved in biological processesthe art: Mehtods of Moleculardegeneration or331D5ranging from hematopoiesis,Biology, 2000, vol. 138:atrophy, including cancerangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9811226Chemokines are a familyChemokine activities can beAbnormal proliferation,chemokineAccessionof related small, secreted proteinsdetermined using assays known inregeneration, degeneration orproteinW59430involved in biological processesthe art: Mehtods of Molecularatrophy, including cancer61164ranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.ChemokineGeneSeqWO9809171Chemokines are a familyChemokine activities can beImmune, Inflammatory, andMCP-4Accessionof related small, secreted proteinsdetermined using assays known ininfectious diseasesW56690involved in biological processesthe art: Mehtods of Molecularranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqFR2751658Chemokines are a familyChemokine activities can beHIV infectionsstromalAccessionof related small, secreted proteinsdetermined using assays known incell-W50766involved in biological processesthe art: Methods in Molecularderivedranging from hematopoiesis,Biology, 2000, vol. 138:chemokine,angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:SDF-1Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.ThymusGeneSeqWO9801557Chemokines are a familyChemokine activities can beImmune and inflammatoryexpressedAccessionof related small, secreted proteinsdetermined using assays known indisorderschemokineW44397involved in biological processesthe art: Methods in Molecular(TECK)ranging from hematopoiesis,Biology, 2000, vol. 138: Chemokineangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot, T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9801557Chemokines are a familyChemokine activities can beImmune and inflammatorychemokineAccessionof related small, secreted proteinsdetermined using assays known indisordersMIP-3W44398involved in biological processesthe art: Methods in Molecularalpharanging from hematopoiesis,Biology, 2000, vol. 138: Chemokineangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot, T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9801557Chemokines are a familyChemokine activities can beImmune and inflammatorychemokineAccessionof related small, secreted proteinsdetermined using assays known indisordersMIP-W44399involved in biological processesthe art: Methods in Molecular3betaranging from hematopoiesis,Biology, 2000, vol. 138: Chemokineangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot, T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9802459Chemokines are a familyChemokine activities can beImmune disorders, respiratorymonocyteAccessionof related small, secreted proteinsdetermined using assays known indisorders, cancerchemotacticW42072involved in biological processesthe art: Methods in Molecularproproteinranging from hematopoiesis,Biology, 2000, vol. 138: Chemokine(MCPP)angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.sequenceMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.Macro-GeneSeqUS5688927/Chemokines are a familyChemokine activities can beImmune, and inflammatoryphage-AccessionsUS5932703of related small, secreted proteinsdetermined using assays known indisorders, cancerderivedW40811involved in biological processesthe art: Methods in Molecularchemokineandranging from hematopoiesis,Biology, 2000, vol. 138: Chemokine(MDC)Y24414angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot, T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.MacrophageGeneSeqUS5932703Chemokines are a familyChemokine activities can beImmune and inflammatoryderivedAccessionof related small, secreted proteinsdetermined using assays known indisorderschemokineY24416involved in biological processesthe art: Methods in Molecularanalogueranging from hematopoiesis,Biology, 2000, vol. 138: ChemokineMDC-eyfyangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot, T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.MacrophageGeneSeqUS5932703Chemokines are a familyChemokine activities can beImmune and inflammatoryderivedAccessionof related small, secreted proteinsdetermined using assays known indisorderschemokineY24413involved in biological processesthe art: Methods in Molecularanalogueranging from hematopoiesis,Biology, 2000, vol. 138: ChemokineMDCangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.(n+1)Members of this family are involved in aProudfoot, T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.MacrophageGeneSeqUS5932703Chemokines are a familyChemokine activities can beImmune and inflammatoryderivedAccessionof related small, secreted proteinsdetermined using assays known indisorderschemokineY24415involved in biological processesthe art: Methods in Molecularanalogueranging from hematopoiesis,Biology, 2000, vol. 138: ChemokineMDC-ylangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot, T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqJP11243960Chemokines are a familyChemokine activities can beAllergic diseases and HIVtype CCAccessionof related small, secreted proteinsdetermined using assays known ininfectionchemokineY43178involved in biological processesthe art: Methods in Moleculareotaxin 3ranging from hematopoiesis,Biology, 2000, vol. 138: Chemokineproteinangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.sequenceMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9946392Chemokines are a familyChemokine activities can beCancer and immune disorders,MCP-3 andAcessionof related small, secreted proteinsdetermined using assays known inparticularly HIV infectionhumanY29893involved in biological processesthe art: Methods in MolecularMuc-1ranging from hematopoiesis,Biology, 2000, vol. 138:coreangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:epitopeMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,(VNT)similarly diverse range of pathologiesand C. A. Power. © Humana Pressfusionincluding inflammation, allergy, tissueInc., Totowa, NJproteinrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9946392Chemokines are a familyChemokine activities can beCancer and immune disorders,IP-10 andAccessionof related small, secreted proteinsdetermined using assays known inparticularly HIV infectionhumanY29894involved in biological processesthe art: Methods in MolecularMuc-1ranging from hematopoiesis,Biology, 2000, vol. 138: Chemokinecoreangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.epitopeMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.(VNT)similarly diverse range of pathologiesPower. © Humana Pressfusionincluding inflammation, allergy, tissueInc., Totowa, NJproteinrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqW09946392Chemokines are a familyChemokine activities can beCancer and immune disorders,IP-10 andAccessionof related small, secreted proteinsdetermined using assays known inparticularly HIV infectionHIV-1Y29897involved in biological processesthe art: Methods in Moleculargp 120ranging from hematopoiesis,Biology, 2000, vol. 138: Chemokinehyper-angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.variableMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.regionsimilarly diverse range of pathologiesPower. © Humana Pressfusionincluding inflammation, allergy, tissueInc., Totowa, NJproteinrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9936540Chemokines are a familychemokine activities can beBreast disease, includingmammaryAccessionsof related small, secreted proteinsdetermined using assays known incancerassociatedY29092 andinvolved in biological processesthe art: Methods in MolecularchemokineY29093ranging from hematopoiesis,Biology, 2000, vol. 138: Chemokine(MACK)angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.proteinMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.Full-similarly diverse range of pathologiesPower. © Humana PressLengthincluding inflammation, allergy, tissueInc., Totowa, NJandrejection, viral infection, and tumorMaturebiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.Tim-1GeneSeqWO9933990Chemokines are a familyChemokine activities can beInflammation due to stimuliproteinAccessionof related small, secreted proteinsdetermined using assays known insuch as heart attacks andY28290involved in biological processesthe art: Methods in Molecularstroke, infection, physicalranging from hematopoiesis,Biology, 2000, vol. 138: Chemokinetrauma, UV or ionizingangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.radiation, burns, frostbite orMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.corrosive chemicalssimilarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9928473 andChemokines are a familyChemokine activities can beHIV infection and cancer,Lkn-1AccessionsWO9928472of related small, secreted proteinsdetermined using assays known inparticularly leukemiaFull-Y17280,involved in biological processesthe art: Methods in MolecularLengthY17274,ranging from hematopoiesis,Biology, 2000, vol. 138: ChemokineandY17281,angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.MatureandMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.proteinY17275similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.N-terminalGeneSeqWO9920759Chemokines are a familyChemokine activities can beInhibit or stimulatemodifiedAccessionof related small, secreted proteinsdetermined using assays known inangiogenesis, inhibit thechemokineY05818involved in biological processesthe art: Methods in Molecularbinding of HIVmet-ranging from hematopoiesis,Biology, 2000, vol. 138: ChemokinehSDF-1angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.alphaMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.N-terminalGeneSeqWO9920759Chemokines are a familyChemokine activities can beInhibit or stimulatemodifiedAccessionof related small, secreted proteinsdetermined using assays known inangiogenesis, inhibit thechemokineY05819involved in biological processesthe art: Methods in Molecularbinding of HIV,met-ranging from hematopoiesis,Biology, 2000, vol. 138: chemokineantiinflammatory;hSDF-1angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.immunosuppressantbetaMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.N-terminalGeneSeqWO9920759Chemokines are a familyChemokine activities can beInhibit or stimulatemodifiedAccessionof related small, secreted proteinsdetermined using assays known inangiogenesis, inhibit thechemokineY05820involved in biological processesthe art: Methods in Molecularbinding of HIV,GroHEK/ranging from hematopoiesis,Biology, 2000, vol. 138: Chemokineantiinflammatory;hSDF-angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.immunosuppressant1alphaMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.N-terminalGeneSeqWO9920759Chemokines are a familyChemokine activities can beInhibit or stimulatemodifiedAccessionof related small, secreted proteinsdetermined using assays known inangiogenesis, inhibit thechemokineY05821involved in biological processesthe art: Methods in Molecularbinding of HIV,GroHEK/ranging from hematopoiesis,Biology, 2000, vol. 138: Chemokineantiinflammatory;hSDF-angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.immunosuppressant1beta.Members of this family are involved in aProudfoot, T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.ChemokineGeneSeqWO9912968Chemokines are a familyChemokine activities can beIncrease or enhance anEotaxinAccessionof related small, secreted proteinsdetermined using assays known ininflammatory response, anY14230involved in biological processesthe art: Methods in Molecularimmune responseranging from hematopoiesis,Bilogy, 2000, vol. 138: Chemokineorhaematopoietic cell-angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.associated activity; treat aMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.vascular indication; Cancer;similarly diverse range of pathologiesPower. © Humana Pressenhance wound healing, toincluding inflammation, allergy, tissueInc., Totowa, NJprevent or treat asthma, organrejection, viral infection, and tumortransplant rejction,biology. The chemokines exert theirrheumatoideffects by acting on a family of sevenarthritis or allergytransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.ChemokineGeneSeqWO9912968Chemokines are a familyChemokine activities can beImmune disorders, VascularhMCP1aAccessionof related small, secreted proteinsdetermined using assays known indisorders, Wound healing,Y14225involved in biological processesthe art: Methods in Molecularcancer, prevent organranging from hematopoiesis,Bilogy, 2000, vol. 138: Chemokinetransplant rejection, Increaseangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.or enhance an inflammatoryMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.response,similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.ChemokineGeneSeqWO9912968Chemokines are a familyChemokine activities can beImmune disorders, VascularhMCP1bAccessionof related small, secreted proteinsdetermined using assays known indisorders, Wound healing,Y14226involved in biological processesthe art: Methods in Molecularcancer, prevent organranging from hematopoiesis,Bilogy, 2000, vol. 138: Chemokinetransplant rejection, Increaseangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.or enhance an inflammatoryMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.response,similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.ChemokineGeneSeqWO9912968Chemokines are a familyChemokine activities can beImmune disorders, VascularhSDF1bAccessionof related small, secreted proteinsdetermined using assays known indisorders, Wound healing,Y14228involved in biological processesthe art: Methods in Molecularcancer, prevent organranging from hematopoiesis,Bilogy, 2000, vol. 138: Chemokinetransplant rejection, Increaseangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.or enhance an inflammatoryMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.response,similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.ChemokineGeneSeqWO9912968Chemokines are a familyChemokine activities can beImmune disorders, VascularhIL-8Accessionof related small, secreted proteinsdetermined using assays known indisorders, Wound healing,Y14229involved in biological processesthe art: Methods in Molecularcancer, prevent organranging from hematopoiesis,Bilogy, 2000, vol. 138: Chemokinetransplant rejection, Increaseangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.or enhance an inflammatoryMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.response,similarly diverse range of pathologiesPower. © Humana Press Inc.,including inflammation, allergy, tissueTotowa, NJ; and Holmes et alrejection, viral infection, and tumor(1991) Science 253, 1278-80.biology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.ChemokineGeneSeqWO9912968Chemokines are a familyChemokine activities can beImmune disorders, VascularhMCP1Accessionof related small, secreted proteinsdetermined using assays known indisorders, Wound healing,Y14222involved in biological processesthe art: Methods in Molecularcancer, prevent organranging from hematopoiesis,Bilogy, 2000, vol. 138: Chemokinetransplant rejection, Increaseangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.or enhance an inflammatoryMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.response,similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.ChemokineGeneSeqWO9912968Chemokines are a familyChemokine activities can beImmune disorders, VascularhMCP2Accessionof related small, secreted proteinsdetermined using assays known indisorders, Wound healing,Y14223involved in biological processesthe art: Methods in Molecularcancer, prevent organranging from hematopoiesis,Bilogy, 2000, vol. 138: Chemokinetransplant rejection, Increaseangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.or enhance an inflammatoryMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.response,similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.ChemokineGeneSeqWO9912968Chemokines are a familyChemokine activities can beImmune disorders, VascularhMCP3Accessionof related small, secreted proteinsdetermined using assays knowndisorders, Wound healing,Y14224involved in biological processesin the art: Methods in Molecularcancer, prevent organranging from hematopoiesis,Bilogy, 2000, vol. 138: Chemokinetransplant rejection, Increaseangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.or enhance an inflammatoryMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.response,similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.C-CGeneSeqEP905240Chemokines are a family of relatedChemokine activities can beInflammatory, Immune andchemokine,Accessionsmall, secreted proteins involved indetermined using assays known ininfectious diseases; pulmonaryMCP2Y05300biological processes ranging fromthe art: Methods in Moleculardiseases and skinhematopoiesis, angiogenesis, andBilogy, 2000, vol. 138: Chemokinedisorders; tumours, andleukocyte trafficking.Protocols. Edited by: A. E. I.angiogenesis-andMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.haematopoiesis-relatedsimilarly diverse range of pathologiesPower. © Humana Pressdiseasesincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viralk infection, and tumorbiology. The chemokines exert their effectsby acting on a family of seventransmembrane G-protein-coupledreceptors. Over 40 human chemokines havebeen described, which bind to ˜17receptors thus far identified.Wild typeGeneSeqEP906954Chemokines are a family of relatedChemokine activities can beInflammatory, Immune andmonocyteAccessionsmall, secreted proteins involved indetermined using assays known ininfectious diseases; pulmonarychemotacticY07233biological processes ranging fromthe art: Methods in Moleculardiseases and skin disorders;protein 2hematopoiesis, angiogenesis, andBilogy, 2000, vol. 138: Chemokinetumours, and angiogenesis-leukocyte trafficking.Protocols. Edited by: A. E. I.and haematopoiesis-relatedMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.diseasessimilarly diverse range of pathologiesPower. ©Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viralk infection, and tumorbiology. The chemokines exert their effectsby acting on a family of seventransmembrane G-protein-coupledreceptors. Over 40 human chemokines havebeen described, which bind to ˜17receptors thus far identified.TruncatedGeneSeqEP906954Chemokines are a familyChemokines activities can beInflammatory, immune andmonocyteAccessionof related small, secreted proteinsdetermined using assays known ininfectious diseases; pulmonrychemotacticY07234involved in biological processesthe art: Methods in Moleculardiseases and skin disorders;protein 2ranging from hematopoiesis,Biology, 2000, vol. 138: Chemokinetumours, and angiogenesis-and(6-76)angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.haematopoiesis-relatedMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.diseasessimilarly diverse range of pathologiesPower, Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.TruncatedGeneSeqEP905241; EP906954Chemokines are a familyChemokines activities can beInflammatory, immune andRANTESAccessionsof related small, secreted proteinsdetermined using assays known ininfectious diseases; pulmonryproteinY07236 andinvolved in biological processesthe art: Methods in Moleculardiseases and skin disorders;(3-68)Y07232ranging from hematopoiesis,Biology, 2000, vol. 138: Chemokinetumours, and angiogenesis-andangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.haematopoiesis-relatedMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.diseasessimilarly diverse range of pathologiesPower, Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.Wild typeGeneSeqEP905241Chemokines are a familyChemokines activities can beInflammatory, immune andmonocyteAccessionof related small, secreted proteinsdetermined using assays known ininfectious diseases; pulmonrychemotacticY07237involved in biological processesthe art: Methods in Moleculardiseases and skin disorders;protein 2ranging from hematopoiesis,Biology, 2000, vol. 138: Chemokinetumours, and angiogenesis-andangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.haematopoiesis-relatedMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.diseasessimilarly diverse range of pathologiesPower, Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.TruncatedGeneSeqEP905241Chemokines are a familyChemokines activities can beInflammatory, immune andmonocyteAccessionof related small, secreted proteinsdetermined using assays known ininfectious diseases; pulmonrychemo-Y07238involved in biological processesthe art: Methods in Moleculardiseases and skin disorders;tacticranging from hematopoiesis,Biology, 2000, vol. 138: Chemokinetumours, and angiogenesis-andproteinangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.haematopoiesis-related2 (6-76)Members of this family are involved in aProudfoot, T. N. C. Wells, and C. A.diseasessimilarly diverse range of pathologiesPower, Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.A partialGeneSeqEP897980Chemokines are a familyChemokines activities can beSoluble CXCR4B receptorCXCR4BAccessionof related small, secreted proteinsdetermined using assays known inpolypeptides may be useful forproteinW97363involved in biological processesthe art: Methods in Molecularinhibiting chemokine activitiesranging from hematopoiesis,Biology, 2000, vol. 138: Chemokineand viral infection.angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot, T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower, Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.InterferonGeneSeqUS5871723Chemokines are a familyChemokines activities can beAngiogenesis, Cancer,gamma-Accessionof related small, secreted proteinsdetermined using assays known inInflammatory and ImmuneinducibleW96709involved in biological processesthe art: Methods in Moleculardisorders, Cardio-Vascularproteinranging from hematopoiesis,Biology, 2000, vol. 138: Chemokinediscorders, Musco-skeletal(IP-10)angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.disordersMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower, Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.A mono-GeneSeqUS5871723Chemokines are a familyChemokines activities can beAngiogenesis, Cancer,kineAccessionof related small, secreted proteinsdetermined using assays known inInflammatory and ImmuneinducedW96710involved in biological processesthe art: Methods in Moleculardisorders, Cardio-Vascularby gamma-ranging from hematopoiesis,Biology, 2000, vol. 138: Chemokinediscorders, Musco-skeletalinter-angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.disordersferonMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.(MIG)similarly diverse range of pathologiesPower, Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.Inter-GeneSeqUS5871723Chemokines are a familyChemokines activities can beAngiogenesis, Cancer,leukin-8Accessionof related small, secreted proteinsdetermined using assays known inInflammatory and Immune(IL-8)W96711involved in biological processesthe art: Methods in Moleculardisorders, Cardio-Vascularprotein.ranging from hematopoiesis,Biology, 2000, vol. 138: Chemokinediscorders, Musco-skeletalangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.disordersMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower, Humana Press Inc., Totowa, NJ;including inflammation, allergy, tissueand Holmes et al (1991) Sciencerejection, viral infection, and tumor253, 1278-80.biology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.EpithelialGeneSeqUS5871723Chemokines are a familyChemokines activities can beAngiogenesis, Cancer,neutrophilAccessionof related small, secreted proteinsdetermined using assays known inInflammatory and ImmuneactivatingW96712involved in biological processesthe art: Methods in Moleculardisorders, Cardio-Vascularprotein-78ranging from hematopoiesis,Biology, 2000, vol. 138: Chemokinediscorders, Musco-skeletal(ENA-78)angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.disordersMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower, Humana Press Inc., Totowa, NJincluding inflammation, allergy, tissuerejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.GrowthGeneSeqUS5871723Chemokines are a familyChemokines activities can beAngiogenesis, Cancer,relatedAccessionof related small, secreted proteinsdetermined using assays known inInflammatory and Immuneoncogene-W96713involved in biological processesthe art: Methods in Moleculardisorders, Cardio-Vascularalpharanging from hematopoiesis,Biology, 2000, vol. 138: Chemokinediscorders, Musco-skeletal(GRO-alpha).angiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.disordersMembers of this family are involved in aProudfoot, T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower, Humana Press Inc., Totowa, NJincluding inflammation, allergy, tissuerejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.GrowthGeneSeqUS5871723Chemokines are a familyChemokine activities can beAngiogenesis, Cancer,relatedAccessionof related small, secreted proteinsdetermined using assays known inInflammatory and Immuneoncogene-W96714involved in biological processesthe art: Methods in Moleculardisorders, Cardio-Vascularbetaranging from hematopoiesis,Biology, 2000, vol. 138:disorders, Musco-skeletal(GRO-beta).angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:disordersMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power, © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.GrowthGeneSeqUS5871723Chemokines are a familyChemokine activities can beAngiogenesis, Cancer,relatedAccessionof related small, secreted proteinsdetermined using assays known inInflammatory and Immuneoncogene-W96715involved in biological processesthe art: Methods in Moleculardisorders, Cardio-Vasculargammaranging from hematopoiesis,Biology, 2000, vol. 138:disorders, Musco-skeletal(GRO-gamma)angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:disordersMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power, © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.A plate-GeneSeqUS5871723Chemokines are a familyChemokine activities can beAngiogenesis, Cancer,let basicAccessionof related small, secreted proteinsdetermined using assays known inInflammatory and ImmuneproteinW96716involved in biological processesthe art: Methods in Moleculardisorders, Cardio-Vascular(PBP)ranging from hematopoiesis,Biology, 2000, vol. 138:disorders, Musco-skeletalangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:disordersMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power, © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.ConnectiveGeneSeqUS5871723Chemokines are a familyChemokine activities can beAngiogenesis, Cancer,tissueAccessionof related small, secreted proteinsdetermined using assays known inInflammatory and ImmuneactivatingS96717involved in biological processesthe art: Methods in Moleculardisorders, Cardio-Vascularprotein-IIIranging from hematopoiesis,Biology, 2000, vol. 138:disorders, Musco-skeletal(CTAP-III)angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:disordersMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power, © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.Beta-GeneSeqUS5871723Chemokines are a familyChemokine activities can beAngiogenesis, Cancer,thrombo-Accessionof related small, secreted proteinsdetermined using assays known inInflammatory and ImmuneglobulinW96718involved in biological processesthe art: Methods in Moleculardisorders, Cardio-Vascularproteinranging from hematopoiesis,Biology, 2000, vol. 138:disorders, Musco-skeletal(beta-TG)angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:disordersMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power, © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.NeutrophilGeneSeqUS5871723Chemokines are a familyChemokine activities can beAngiogenesis, Cancer,activatingAccessionof related small, secreted proteinsdetermined using assays known inInflammatory and Immunepeptide-2W96719involved in biological processesthe art: Methods in Moleculardisorders, Cardio-Vascular(NAP-2)ranging from hematopoiesis,Biology, 2000, vol. 138:disorders, Musco-skeletalangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:disordersMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power, © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.Granulo-GeneSeqUS5871723Chemokines are a familyChemokine activities can beAngiogenesis, Cancer,cyteAccessionof related small, secreted proteinsdetermined using assays known inInflammatory and Immunechemo-W96720involved in biological processesthe art: Methods in Moleculardisorders, Cardio-Vasculartacticranging from hematopoiesis,Biology, 2000, vol. 138:disorders, Musco-skeletalprotein-2angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:disorders(GCP-2)Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power, © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqEP887409Chemokines are a familyChemokine activities can beImmune disorders, viral,chemokineAccessionof related small, secreted proteinsdetermined using assays known inparasitic, fungal orMIG-betaW90124involved in biological processesthe art: Methods in Molecularbacterialproteinranging from hematopoiesis,Biology, 2000, vol. 138:infections, Cancer;angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:autoimmune diseases orMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,transplant rejectionsimilarly diverse range of pathologiesand C. A. Power, © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9854326Chemokines are a familyChemokine activities can beImmune disorders, cancer,ZCHEMO-8Accessionof related small, secreted proteinsdetermined using assays known inmyelopoietic disorders,W82716involved in biological processesthe art: Methods in Molecularautoimmuneranging from hematopoiesis,Biology, 2000, vol. 138:disorders andangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:immunodeficiencies,Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,Inflammatory and infectioussimilarly diverse range of pathologiesand C. A. Power, © Humana Pressdiseases, Vascularincluding inflammation, allergy, tissueInc., Totowa, NJdisorders,rejection, viral infection, and tumorwound healingbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9854326Chemokines are a familyChemokine activities can beImmune disorders, cancer,Act-2Accessionof related small, secreted proteinsdetermined using assays known inmyelopoietic disorders,proteinW82717involved in biological processesthe art: Methods in Molecularautoimmuneranging from hematopoiesis,Biology, 2000, vol. 138:disorders andangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:immunodeficiencies,Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,Inflammatory and infectioussimilarly diverse range of pathologiesand C. A. Power, © Humana Pressdiseases, Vascularincluding inflammation, allergy, tissueInc., Totowa, NJdisorders,rejection, viral infection, and tumorwound healingbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9854326Chemokines are a familyChemokine activities can beImmune disorders, cancer,SISDAcessionof related small, secreted proteinsdetermined using assays known inmyelopoietic disorders,proteinW82720involved in biological processesthe art: Methods in Molecularautoimmuneranging from hematopoiesis,Biology, 2000, vol. 138:disorders andangiogenesis, and leukocyte trafficking.Chemokine Protocols, Edited by:immunodeficiencies,Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,Inflammatory and infectioussimilarly diverse range of pathologiesand C. A. Power. © Humana Pressdiseases, Vascularincluding inflammation, allergy, tissueInc., Totowa, NJdisorders,rejection, viral infection, and tumorwound healingbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9854326Chemokines are a familyChemokine activities can beImmune disorders, cancer,M110Accessionof related small, secreted proteinsdetermined using assays known inmyelopoietic disorders,proteinW82721involved in biological processesthe art: Mehtods of Molecularautoimmuneranging from hematopoiesis,Biology, 2000, vol. 138:disorders andangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:immunodeficiencies,Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,Inflammatory and infectioussimilarly diverse range of pathologiesand C. A. Power © Humana Pressdiseases, Vascularincluding inflammation, allergy, tissueInc., Totowa, NJdisorders,rejection, viral infection, and tumorwound healingbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqW09854326Chemokines are a familyChemokine activities can beImmune disorders, cancer,M11AAccessionof related small, secreted proteinsdetermined using assays known inmyelopoietic disorders,proteinW82722involved in biological processesthe art: Mehtods of Molecularautoimmuneranging from hematopoiesis,Biology, 2000, vol. 138:disorders andangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:immunodeficiencies,Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,Inflammatory and infectioussimilarly diverse range of pathologiesand C. A. Power © Humana Pressdiseases, Vascularincluding inflammation, allergy, tissueInc., Totowa, NJdisorders,rejection, viral infection, and tumorwound healingbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9854326Chemokines are a familyChemokine activities can beImmune disorders, cancer,CCC3Accessionof related small, secreted proteinsdetermined using assays known inmyelopoietic disorders,proteinW82723involved in biological processesthe art: Mehtods of Molecularautoimmuneranging from hematopoiesis,Biology, 2000, vol. 138:disorders andangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:immunodeficiencies,Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,Inflammatory and infectioussimilarly diverse range of pathologiesand C. A. Power © Humana Pressdiseases, Vascularincluding inflammation, allergy, tissueInc., Totowa, NJdisorders,rejection, viral infection, and tumorwound healingbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.A humanGeneSeqWO9856818Chemokines are a familyChemokine activities can beCancer, wound healingL105Accessionof related small, secreted proteinsdetermined using assays known inchemokineW87588involved in biological processesthe art: Mehtods of Moleculardesignatedranging from hematopoiesis,Biology, 2000, vol. 138:huL105_3.angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.A humanGeneSeqWO9856818Chemokines are a familyChemokine activities can beCancer, wound healingL105Accessionof related small, secreted proteinsdetermined using assays known inchemokineW87589involved in biological processesthe art: Mehtods of Moleculardesignatedranging from hematopoiesis,Biology, 2000, vol. 138:huL105_7.angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9848828Chemokines are a familyChemokine activities can beInfectious diseases,matureAccessionof related small, secreted proteinsdetermined using assays known insepsisgro-alphaW81498involved in biological processesthe art: Mehtods of Molecularpoly-ranging from hematopoiesis,Biology, 2000, vol. 138:peptideangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:used toMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,treatsimilarly diverse range of pathologiesand C. A. Power © Humana Presssepsisincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO9848828Chemokines are a familyChemokine activities can beInfectious diseases,matureAccessionof related small, secreted proteinsdetermined using assays known insepsisgro-gammaW81500involved in biological processesthe art: Mehtods of Molecularpoly-ranging from hematopoiesis,Biology, 2000, vol. 138:peptideangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:used toMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,treatsimilarly diverse range of pathologiesand C. A. Power © Humana Presssepsisincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0053635Chemokines are a familyChemokine activities can beInflammatory disorders,thymusAccessionsof related small, secreted proteinsdetermined using assays known incancer,expressedB19607involved in biological processesthe art: Mehtods of MolecularImmune and vascularchemokineandranging from hematopoiesis,Biology, 2000, vol. 138:disordersTECK andB19608angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:TECKMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,variantsimilarly diverse range of pathologiesand C. A. Power © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0042071Chemokines are a familyChemokine activities can beAutoimmune disorders,chemokineAccessionof related small, secreted proteinsdetermined using assays known inImmune, Vascular andSDF1alphaB15791involved in biological processesthe art: Mehtods of MolecularInflammatory disordersranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0042071Chemokines are a familyChemokine activities can beAutoimmune disorders,chemokineAccessionof related small, secreted proteinsdetermined using assays known inImmune, Vascular andGROalphaB15793involved in biological processesthe art: Methods in MolecularInflammatory diordersranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot; T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0042071Chemokines are a familyChemokine activities can beAutoimmune disorders,chemokineAccessionof related small, secreted proteinsdetermined using assays known inImmune, Vascular andeotaxinB15794involved in biological processesthe art: Methods in MolecularInflammatory disordersranging from hematopoiesis,Biology, 2000, vol. 138: Chemokineangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot; T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0042071Chemokines are a familyChemokine activities can beAutoimmune disorders,chemokineAccessionof related small, secreted proteinsdetermined using assays known inImmune, Vascular andMIGB15803involved in biological processesthe art: Methods in MolecularInflammatory disordersranging from hematopoiesis,Biology, 2000, vol. 138: Chemokineangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot; T. N. C. Wells, and C.Asimilarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0042071Chemokines are a familyChemokine activities can beAutoimmune disorders,chemokineAccessionof related small, secreted proteinsdetermined using assays known inImmune, Vascular andPF4B15804involved in biological processesthe art: Methods in MolecularInflammatory disordersranging from hematopoiesis,Biology, 2000, vol. 138: Chemokineangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot; T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0042071Chemokines are a familyChemokine activities can beAutoimmune disorders,chemokineAccessionof related small, secreted proteinsdetermined using assays known inImmune, Vascular andI-309B15805involved in biological processesthe art: Methods in MolecularInflammatory disordersranging from hematopoiesis,Biology, 2000, vol. 138: Chemokineangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot; T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0042071Chemokines are a familyChemokine activities can beAutoimmune disorders,chemokineAccessionof related small, secreted proteinsdetermined using assays known inImmune, Vascular andHCC-1B15806involved in biological processesthe art: Methods in MolecularInflammatory disordersranging from hematopoiesis,Biology, 2000, vol. 138: Chemokineangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot; T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0042071Chemokines are a familyChemokine activities can beAutoimmune disorders,chemokineAccessionof related small, secreted proteinsdetermined using assays known inImmune, Vascular andC10B15807involved in biological processesthe art: Methods in MolecularInflammatory disordersranging from hematopoiesis,Biology, 2000, vol. 138: Chemokineangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot; T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0042071Chemokines are a familyChemokine activities can beAutoimmune disorders,chemokineAccessionof related small, secreted proteinsdetermined using assays known inImmune, Vascular andCCR-2B15808involved in biological processesthe art: Methods in MolecularInflammatory disordersranging from hematopoiesis,Biology, 2000, vol. 138: Chemokineangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot; T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0042071Chemokines are a familyChemokine activities can beAutoimmune disorders,chemokineAccessionof related small, secreted proteinsdetermined using assays known inImmune, Vascular andENA-78B15809involved in biological processesthe art: Methods in MolecularInflammatory disordersranging from hematopoiesis,Biology, 2000, vol. 138: Chemokineangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot; T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0042071Chemokines are a familyChemokine activities can beAutoimmune disorders,chemokineAccessionof related small, secreted proteinsdetermined using assays known inImmune, Vascular andGRObetaB15810involved in biological processesthe art: Methods in MolecularInflammatory disordersranging from hematopoiesis,Biology, 2000, vol. 138: Chemokineangiogenesis, and leukocyte trafficking.Protocols. Edited by: A. E. I.Members of this family are involved in aProudfoot; T. N. C. Wells, and C. A.similarly diverse range of pathologiesPower. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0042071Chemokines are a familyChemokine activities can beAutoimmune disorders,chemokineAccessionof related small, secreted proteinsdetermined using assays known inImmune, Vascular andIP-10B15811involved in biological processesthe art: Methods in MolecularInflammatory disordersranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0042071Chemokines are a familyChemokine activities can beAutoimmune disorders,chemokineAccessionof related small, secreted proteinsdetermined using assays known inImmune, Vascular andSDF1betaB15812involved in biological processesthe art: Methods in MolecularInflammatory disordersranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0042071Chemokines are a familyChemokine activities can beAutoimmune disorders,chemokineAccessionof related small, secreted proteinsdetermined using assays known inImmune, Vascular andGRO alphaB15813involved in biological processesthe art: Methods in MolecularInflammatory disordersranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0042071Chemokines are a familyChemokine activities can beAutoimmune disorders,chemokineAccessionof related small, secreted proteinsdetermined using assays known inImmune, Vascular andMIP1betaB15831involved in biological processesthe art: Methods in MolecularInflammatory disordersranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.A humanGeneSeqUS6096300Chemokines are a familyChemokine activities can beCancerC-CAccessionof related small, secreted proteinsdetermined using assays known inchemokineB07939involved in biological processesthe art: Methods in Moleculardesignatedranging from hematopoiesis,Biology, 2000, vol. 138:exodusangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqUS6084071Chemokines are a familyChemokine activities can beChemotaxis, Gene Therapy,chemokineAccessionof related small, secreted proteinsdetermined using assays known inWound healingL105_7Y96922involved in biological processesthe art: Methods in Molecularranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqUS6084071Chemokines are a familyChemokine activities can beChemotaxis, Gene Therapy,chemokineAccessionof related small, secreted proteinsdetermined using assays known inWound healingL105_3Y96923involved in biological processesthe art: Methods in Molecularranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0038706Chemokines are a familyChemokine activities can beCancer, Vascular and ImmunesecondaryAccessionof related small, secreted proteinsdetermined using assays known indisorderslymphoidB01434involved in biological processesthe art: Methods in Molecularchemokineranging from hematopoiesis,Biology, 2000, vol. 138:(SLC)angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0029439Chemokines are a familyChemokine activities can beImmune and Inflammatorynon-ELRAccessionof related small, secreted proteinsdetermined using assays known indisorders, Cancer, HaemostaticCXCY96310involved in biological processesthe art: Methods in Molecularand thrombolyticchemokineranging from hematopoiesis,Biology, 2000, vol. 138:activityH174angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0029439Chemokines are a familyChemokine activities can beImmune and Inflammatorynon-ELRAccessionof related small, secreted proteinsdetermined using assays known indisorders, Cancer, haemostaticCXCY96311involved in biological processesthe art: Methods in Molecularand thrombolytic activitychemokineranging from hematopoiesis,Biology, 2000, vol. 138:IP10angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0029439Chemokines are a familyChemokine activities can beImmune and Inflammatorynon-ELRAccessionof related small, secreted proteinsdetermined using assays known indisorders, Cancer, haemostaticCXCY96313involved in biological processesthe art: Methods in Molecularand thrombolytic activitychemokineranging from hematopoiesis,Biology, 2000, vol. 138:Migangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0028035Chemokines are a familyChemokine activities can beCancer, wound healing,chemokineAccessionof related small, secreted proteinsdetermined using assays known ininflammatory andCkbeta-7Y96280involved in biological processesthe art: Methods in Molecularimmunoregulatoryranging from hematopoiesis,Biology, 2000, vol. 138:disordersangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0028035Chemokines are a familyChemokine activities can beCancer, wound healing,chemokineAccessionof related small, secreted proteinsdetermined using assays known ininflammatory andMIP-1alphaY96281involved in biological processesthe art: Methods in Molecularimmunoregulatoryranging from hematopoiesis,Biology, 2000, vol. 138:disordersangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGenSeqWO0028035Chemokines are a familyChemokine activities can beCancer, wound healing,matureAccessionof related small, secreted proteinsdetermined using assays known ininflammatory andchemokineY96282involved in biological processesthe art: Methods in MolecularimmunoregulatoryCkbeta-7ranging from hematopoiesis,Biology, 2000, vol. 138:disorders(optionallyangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:truncated)Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqWO0018431Chemokines are a familyChemokine activities can beSoluble CXCR3 polypeptideschemokineAccessionof related small, secreted proteinsdetermined using assays known inmay be useful for inhibitingreceptorY79372involved in biological processesthe art: Methods in Molecularchemokine activities and viralCXCR3ranging from hematopoiesis,Biology, 2000, vol. 138:infection.angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqUS6043086Chemokines are a familyChemokine activities can beNeurological disorders,neuro-Accessionof related small, secreted proteinsdetermined using assays known inImmune and respiratorytactinY53259involved in biological processesthe art: Methods in Moleculardisorderschemokineranging from hematopoiesis,Biology, 2000, vol. 138:likeangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:domainMembers of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqJP11302298Chemokines are a familyChemokine activities can beCancer and infectiousCC typeAccessionof related small, secreted proteinsdetermined using assays known indiseaseschemokineY57771involved in biological processesthe art: Methods in Molecularinter-ranging from hematopoiesis,Biology, 2000, vol. 138:leukin Cangiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.HumanGeneSeqUS6153441Chemokines are a familyChemokine activities can beCancer, Auto-immune andCKbeta-9Accessionof related small, secreted proteinsdetermined using assays known ininflammatory disorders,B50860involved in biological processesthe art: Methods in MolecularCardiovascular disordersranging from hematopoiesis,Biology, 2000, vol. 138:angiogenesis, and leukocyte trafficking.Chemokine Protocols. Edited by:Members of this family are involved in aA. E. I. Proudfoot, T. N. C. Wells,similarly diverse range of pathologiesand C. A. Power. © Humana Pressincluding inflammation, allergy, tissueInc., Totowa, NJrejection, viral infection, and tumorbiology. The chemokines exert theireffects by acting on a family of seventransmembrane G-protein coupled receptors.Over 40 human chemokines have beendescribed, which bind to ˜17receptors thus far identified.Prepro-GeneSeqWO9637608Apoa-1 participates in the reverseLipid binding activity can beUseful for cardiovascularapolipo-Accessiontransport of cholesterol from tissuesdetermined using assays known indisorders, cholesterolproteinW08602to the liver for excretion bythe art, such as, for example, thedisorders, and“paris”promoting cholesterol effluxCholesterol Efflux Assays ofHyperlipidaemiavariantfrom tissues and by acting as aTakahaski et al., P.N.A.S., Vol. 96,cofactor for the lecithin cholesterolIssue 20, 11358-11363, Sep.acyltransferase (lcat).28, 1999.Prepro-5, 721,Apoa-1 participates in the reverseLipid binding activity can beUseful for cardiovascularapolipo-114transport of cholesterol from tissuesdetermined using assays known indisorders, cholesterolproteinto the liver for excretion bythe art, such as, for example, thedisorders, and“milano”promoting cholesterol effluxCholesterol Efflux Assays ofHyperlipidaemiavariantfrom tissues and by acting as aTakahaski et al., P.N.A.S., Vol. 96,cofactor for the lecithin cholesterolIssue 20, 11358-11363, Sep.acyltransferase (lcat).28, 1999.Glyco-GeneSeqWO9628169Naturally produced femaleGlycodelin-A activity can beNaturally deriveddelin-A;Accessioncontraceptive that is removeddetermined using the hemizonacontraceptive useful for thePro-W00289rapidly from the bodyassay as described in Oehninger, S.,prevention of pregnancy.gesterone-following 2-3 days production.Coddington, C. C., Hodgen, G. D., andassociatedUses include contraceptionSeppala, M (1995) Fertil.endometrialSteril. 63, 377-383.proteinNOGO-AGenbankNOGO polypeptides are potentInhibition of Neurite outgrowth.NOGO-A polypeptideAccessioninhibitors of neurite growth.Antagonists to NOGO polypeptidesantagonists are useful for theCAB99248may promote the outgrowth ofpromotion of neural growth,neurites, thus inducingwhich could be useful in theregeneration of neurons.treatment of neural disordersand dysfunction due todegenerative diseases ortrauma; useful in the treatmentof neoplastic diseases of theCNS; induce regeneration ofneurons or to promote thestructural plasticity of theCNS.NOGO-BGenbankNOGO polypeptides are potentInhibition of Neurite outgrowth.NOGO-B polypeptideAccessioninhibitors of neurite growth.Antagonists to NOGO polypeptidesantagonists are useful for theCAB99249may promote the outgrowth ofpromotion of neural growth,neurites, thus inducing regenerationwhich could be useful in theof neurons.treatment of neural disordersand dysfunction due todegenerative diseases ortrauma; useful in the treatmentof neoplastic diseases of theCNS; induce regeneration ofneurons or to promote thestructural plasticity of theCNS.NOGO-CGenbankNOGO polypeptides are potentInhibition of Neurite outgrowth.NOGO-C polypeptideAccessioninhibitors of neurite growth.Antagonists to NOGO polypeptidesantagonists are useful for theCAB99250may promote the outgrowth ofpromotion of neural growth,neurites, thus inducing regenerationwhich could be useful in theof neurons.treatment of neural disordersand dysfunction due todegenerative diseases ortrauma; useful in the treatmentof neoplastic diseases of theCNS; induce regeneration ofneurons or to promote thestructural plasticity of theCNS.NOGO-66GenbankNOGO polypeptides are potentInhibition of Neurite outgrowth byNOGO-66 receptorReceptorAccessioninhibitors of neurite growth,mediating the biological effects ofpolypeptides are useful for theAAG53612and are thought to mediateNOGO polypeptides. Solublepromotion of neural growth,their effects through the NOGO-66NOGO-66 receptor polypeptideswhich could be useful in theReceptor.may promote the outgrowth oftreatment of neural disordersneurites, thus inducing regenerationand dysfunction due toof neurons.degenerative diseases ortrauma; useful in the treatmentof neoplastic diseases of theCNS; induce regeneration ofneurons or to promote thestructural plasticity of theCNS.AntibodiesUS5416197These antibodies are useful for theCollapsin activity, which is thoughtUseful for the promotion ofspecificpromotion of neurite outgrowthto inhibit the outgrowth of neurites,neural growth, which could beforcan be assayed in the presence ofuseful in the treatment ofcollapsinantibodies specific for collapsingneural disorders andusing assays known in the art, suchdysfunction due toas, for example, the collapse assaydegenerative diseases ordisclosed by Luo et al., Cell 1993trauma.Oct. 22; 75(2):217-27HumanizedWO9845331These agents have anti-inflammatoryVEGF activity can be determinedPromotion of growth andAnti-and anti-cancer applicationsusing assays known in the art, suchproliferation of cells, such asVEGFas those disclosed in Internationalvascular endothelial cells.Antibodies,Publication No. WO0045835, forAntagonists may be useful asandexample.anti-angiogenic agents, andfragmentsmay be applicable for cancerthereofHumanizedWO0029584These agents have anti-inflammatoryVEGF activity can be determinedPromotion of growth andAnti-and anti-cancer applicationsusing assays known in the art, suchproliferation of cells, such asVEGFas those disclosed in Internationalvascular endothelial cells.Antibodies,Publication No. WO0045835, forAntagonists may be useful asandexample.anti-angiogenic agents, andfragmentsmay be applicable for cancerthereofMembraneGeneSeq.WO9963088Cancer, Immune DisordersThese proteins can be used forActivities can be determinedboundAccessionlinking bioactive molecules to cellsusing assay known in the art,proteinsY66631-and for modulating biologicalsuch as, for example, theY66765activities of cells, using theassays disclosed inpolypeptides for specific targeting.International Publication No.The polypeptide targeting can beWO0121658.used to kill the target cells, e.g. forthe treatment of cancers. Theseproteins are useful for the treatmentof immune system disorders.SecretedGenSeqWO0053756Cancer, Immune DisordersThese proteins can be used forActivities can be determinedandAccessionlinking bioactive molecules to cellsusing assay known in the art,Trans-B44241-and for modulating biologicalsuch as, for example, themembraneB44334activities of cells, using theassays disclosed inpoly-polypeptides for specific targeting.International Publication No.peptidesThe polypeptide targeting can beWO0121658used to kill the target cells, e.g. forthe treatment of cancers. Theseproteins are useful for the treatmentof immune system disorders.SecretedGeneSeqWO9946281Cancer, Immune DisordersThese proteins can be used forActivities can be determinedandAccessionlinking bioactive molecules to cellsusing assay known in the art,Trans-Y41685-and for modulating biologicalsuch as, for example, themembraneY41774activities of cells, using theassays disclosed inpoly-polypeptides for specific targeting.International Publication No.peptidesThe polypeptide targeting can beWO0121658used to kill the target cells, e.g. forthe treatment of cancers. Theseproteins are useful for the treatmentof immune system disorders.

[0191] Delivery of a Drug or Therapeutic Protein to the Inside of a Cell and/or Across the Blood Brain Barrier (BBB)

[0192] Within the scope of the invention, the modified transferrin fusion proteins may be used as a carrier to deliver a molecule or small molecule therapeutic complexed to the ferric ion of transferrin to the inside of a cell or across the blood brain barrier. In these embodiments, the Tf fusion protein will typically be engineered or modified to inhibit, prevent or remove glycosylation to extend the serum half-life of the fusion protein and/or therapeutic protein portion. The addition of a targeting peptide or, for example, a single chain antibody is specifically contemplated to further target the Tf fusion protein to a particular cell type, e.g., a cancer cell.

[0193] In one embodiment, the iron-containing, anti-anemic drug, ferric-sorbitol-citrate complex is loaded onto a modified Tf fusion protein of the invention. Ferric-sorbitol-citrate (FSC) has been shown to inhibit proliferation of various murine cancer cells in vitro and cause tumor regression in vivo, while not having any effect on proliferation of non-malignant cells (Poljak-Blazi et al. (June 2000) Cancer Biotherapy and Radiopharmaceuticals (United States), 15/3:285-293).

[0194] In another embodiment, the antineoplastic drug adriamycin (Doxorubicin) and/or the chemotherapeutic drug bleomycin, both of which are known to form complexes with ferric ion, is loaded onto a Tf fusion protein of the invention. In other embodiments, a salt of a drug, for instance, a citrate or carbonate salt, may be prepared and complexed with the ferric iron that is then bound to Tf. As tumor cells often display a higher turnover rate for iron; transferrin modified to carry at least one anti-tumor agent, may provide a means of increasing agent exposure or load to the tumor cells. (Demant, E. J, (1983) Eur. J. of Biochem. 137/(1-2):113-118; Padbury et al. (1985) J. Biol. Chem. 260/13:7820-7823).

[0195] Pharmaceutical Formulations and Treatment Methods

[0196] The modified fusion proteins of the invention may be administered to a patient in need thereof using standard administration protocols. For instance, the modified Tf fusion proteins of the present invention can be provided alone, or in combination, or in sequential combination with other agents that modulate a particular pathological process. As used herein, two agents are said to be administered in combination when the two agents are administered simultaneously or are administered independently in a fashion such that the agents will act at the same or near the same time.

[0197] The agents of the present invention can be administered via parenteral, subcutaneous, intravenous, intramuscular, intraperitoneal, transdermal and buccal routes. For example, an agent may be administered locally to a site of injury via microinfusion. Alternatively, or concurrently, administration may be noninvasive by either the oral, inhalation, nasal, or pulmonary route. The dosage administered will be dependent upon the age, health, and weight of the recipient, kind of concurrent treatment, if any, frequency of treatment, and the nature of the effect desired.

[0198] The present invention further provides compositions containing one or more fusion proteins of the invention. While individual needs vary, determination of optimal ranges of effective amounts of each component is within the skill of the art. Typical dosages comprise about 1 pg/kg to about 100 mg/kg body weight. The preferred dosages for systemic administration comprise about 100 ng/kg to about 100 mg/kg body weight or about 100-200 mg of protein/dose. The preferred dosages for direct administration to a site via microinfusion comprise about 1 ng/kg to about 1 mg/kg body weight. When administered via direct injection or microinfusion, modified fusion proteins of the invention may be engineered to exhibit reduced or no binding of iron to prevent, in part, localized iron toxicity.

[0199] In addition to the pharmacologically active fusion protein, the compositions of the present invention may contain suitable pharmaceutically acceptable carriers comprising excipients and auxiliaries that facilitate processing of the active compounds into preparations which can be used pharmaceutically for delivery to the site of action. Suitable formulations for parenteral administration include aqueous solutions of the active compounds in water-soluble form, for example, water-soluble salts. In addition, suspensions of the active compounds as appropriate oily injection suspensions may be administered. Suitable lipophilic solvents or vehicles include fatty oils, for example, sesame oil, or synthetic fatty acid esters, for example, ethyl oleate or triglycerides. Aqueous injection suspensions may contain substances which increase the viscosity of the suspension include, for example, sodium carboxymethyl cellulose, sorbitol and dextran. Optionally, the suspension may also contain stabilizers. Liposomes can also be used to encapsulate the agent for delivery into the cell.

[0200] The pharmaceutical formulation for systemic administration according to the invention may be formulated for enteral, parenteral or topical administration. Indeed, all three types of formulations may be used simultaneously to achieve systemic administration of the active ingredient. Suitable formulations for oral administration include hard or soft gelatin capsules, pills, tablets, including coated tablets, elixirs, suspensions, syrups or inhalations and controlled release forms thereof.

[0201] In practicing the methods of this invention, the agents of this invention may be used alone or in combination, or in combination with other therapeutic or diagnostic agents. In certain preferred embodiments, the compounds of this invention may be co-administered along with other compounds typically prescribed for these conditions according to generally accepted medical practice. The compounds of this invention can be utilized in vivo, ordinarily in mammals, such as humans, sheep, horses, cattle, pigs, dogs, cats, rats and mice, or in vitro.

[0202] Modified fusion proteins of the present invention may be used in the diagnosis, prognosis, prevention and/or treatment of diseases and/or disorders relating to diseases and disorders of the endocrine system, the nervous system, the immune system, respiratory system, cardiovascular system, reproductive system, digestive system, diseases and/or disorders relating to cell proliferation, and/or diseases or disorders relating to the blood.

[0203] In yet other embodiments of the invention, modified Tf fusion proteins may be used in the diagnosis, prognosis, prevention and/or treatment of diseases and/or disorders relating to diseases and disorders known to be associated with or treatable by therapeutic protein moieties as known in the art and exemplified by PCT Patent Publication Nos. WO 01/79258, WO 01/77137, WO 01/79442, WO 01/79443, WO 01/79444 and WO 01/79480, all of which are herein incorpoated by reference in their entirety. Accordingly, the present invention encompasses a method of treating a disease or disorder listed in the “Preferred Indication Y” column of Table 1 comprising administering to a patient in which such treatment, prevention or amelioration is desired a modified transferrin fusion protein of the invention that comprises a therapeutic protein portion corresponding to a therapeutic protein disclosed in the “Therapeutic Protein X” column of Table 1 in an amount effective to treat, prevent or ameliorate the disease or disorder.

[0204] In certain embodiments, a transferrin fusion protein of the present invention may be used to diagnose and/or prognose diseases and/or disorders.

[0205] Modified transferrin fusion proteins of the invention and polynucleotides encoding transferrin fusion proteins of the invention may be useful in treating, preventing, diagnosing and/or prognosing diseases, disorders, and/or conditions of the immune system. Moreover, fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention can be used as a marker or detector of a particular immune system disease or disorder.

[0206] In a preferred embodiment fusion proteins of the invention and/or polynucleotides encoding modified transferrin fusion proteins of the invention could be used as an agent to boost immunoresponsiveness among immunodeficient individuals. In specific embodiments, fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention could be used as an agent to boost immunoresponsiveness among B cell and/or T cell immunodeficient individuals.

[0207] The modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be useful in treating, preventing, diagnosing, and/or prognosing autoimmune disorders. Many autoimmune disorders result from inappropriate recognition of self as foreign material by immune cells. This inappropriate recognition results in an immune response leading to the destruction of the host tissue. Therefore, the administration of fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention, that can inhibit an immune response, particularly the proliferation, differentiation, or chemotaxis of T-cells, may be an effective therapy in preventing autoimmune disorders.

[0208] Modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be useful in treating, preventing, prognosing, and/or diagnosing diseases, disorders, and/or conditions of hematopoietic cells. Transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention could be used to increase differentiation and proliferation of hematopoietic cells, including the pluripotent stem cells, in an effort to treat or prevent those diseases, disorders, and/or conditions associated with a decrease in certain (or many) types hematopoietic cells, including but not limited to, leukopenia, neutropenia, anemia, and thrombocytopenia.

[0209] Alternatively, modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention could be used to increase differentiation and proliferation of hematopoietic cells, including the pluripotent stem cells, in an effort to treat or prevent those diseases, disorders, and/or conditions associated with an increase in certain (or many) types of hematopoietic cells, including but not limited to, histiocytosis.

[0210] Allergic reactions and conditions, such as asthma (particularly allergic asthma) or other respiratory problems, may also be treated, prevented, diagnosed and/or prognosing and using modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention. Moreover, these molecules can be used to treat, prevent, prognose, and/or diagnose anaphylaxis, hypersensitivity to an antigenic molecule, or blood group incompatibility.

[0211] Additionally, modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention, may be used to treat, prevent, diagnose and/or prognose IgE-mediated allergic reactions. Such allergic reactions include, but are not limited to, asthma, rhinitis, and ecizema. In specific embodiments, fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be used to modulate IgE concentrations in vitro or in vivo.

[0212] Moreover, modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention have uses in the diagnosis, prognosis, prevention, and/or treatment of inflammatory conditions. For example, since fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may inhibit the activation, proliferation, and/or differentiation of cells involved in an inflammatory response, these molecules can be used to prevent and/or treat chronic and acute inflammatory conditions. Such inflammatory conditions include, but are not limited to, for example, inflammation associated with infection (e.g., septic shock, sepsis, or systemic inflammatory response syndrome), ischemia-reperfusion injury, endotoxin lethality, complement-mediated hyperacute rejection, nephritis, cytokine or chemokine induced lung injury, inflammatory bowel disease, Crohn's disease, over production of cytokines (e.g., TNF or IL-1.), respiratory disorders (e.g., asthma and allergy); gastrointestinal disorders (e.g., inflammatory bowel disease); cancers (e.g., gastric, ovarian, lung, bladder, liver, and breast); CNS disorders (e.g., multiple sclerosis; ischemic brain injury and/or stroke, traumatic brain injury, neurodegenerative disorders (e.g., Parkinson's disease and Alzheizmer's disease); AIDS-related dementia; and prion disease); cardiovascular disorders (e.g., atherosclerosis, myocarditis, cardiovascular disease, and cardiopulmonary bypass complications); as well as many additional diseases, conditions, and disorders that are characterized by inflammation (e.g., hepatitis, rheumatoid arthritis, gout, trauma, pancreatitis, sarcoidosis, dermatitis, renal ischemia-reperfusion injury, Grave's disease, systemic lupus erythematosus, diabetes mellitus, and allogenic transplant rejection).

[0213] Because inflammation is a fundamental defense mechanism, inflammatory disorders can effect virtually any tissue of the body. Accordingly, modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention, have uses in the treatment of tissue-specific inflammatory disorders, including, but not limited to, adrenalitis, alveolitis, angiocholecystitis, appendicitis, balanitis, blepharitis, bronchitis, bursitis, carditis, cellulitis, cervicitis, cholecystitis, chorditis, cochiftis, colitis, conjunctivitis, cystitis, dermatitis, diverticulitis, encephalitis, endocarditis, esophagitis, eustachitis, fibrositis, folliculitis, gastritis, gastroenteritis, gingivitis, glossitis, hepatosplenitis, keratitis, labyrinthitis, laryngitis, lymphangitis, mastitis, media otitis, meningitis, metritis, mucitis, myocarditis, myosititis, myringitis, nephritis, neuritis, orchitis, osteochondritis, otitis, pericarditis, peritendonitis, peritonitis, pharyngitis, phlebitis, poliomyelitis, prostatititis, Pulpitis, retinitis, rhinitis, salpingitis, scleritis, sclerochoroiditis, scrotitis, sinusitis, spondylitis, steatitis, stomatitis, synovitis, syringitis, tendonitis, tonsillitis, urethritis, and vaginitis.

[0214] In specific embodiments, modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention, are useful to diagnose, prognose, prevent, and/or treat organ transplant rejections and graft-versus-host disease. Organ rejection occurs by host immune cell destruction of the transplanted tissue through an immune response. Similarly, an immune response is also involved in GVHD, but, in this case, the foreign transplanted immune cells destroy the host tissues. Polypeptides, antibodies, or polynucleotides of the invention, and/or agonists or antagonists thereof, that inhibit an immune response, particularly the activation, proliferation, differentiation, orchemotaxis of T-cells, may be an effective therapy in preventing organ rejection or GVHD.

[0215] In another specific embodiment, modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention are used as an adjuvant to enhance anti-viral immune responses. Anti-viral immune responses that may be enhanced using the compositions of the invention as an adjuvant, include virus and virus associated diseases or symptoms described herein or otherwise known in the art. In specific embodiments, the compositions of the invention are used as an adjuvant to enhance an immune response to a virus, disease, or symptom selected from the group consisting of AIDS, meningitis, Dengue, EBV, and hepatitis (e.g., hepatitis B). In another specific embodiment, the compositions of the invention are used as an adjuvant to enhance an immune response to a virus, disease, or symptom selected from the group consisting of: HIV/AIDS, respiratory syncytial virus, Dengue, rotavirus, Japanese B encephalitis, influenza A and B, parainfluenza, measles, cytomegalovirus, rabies, Junin, Chikungunya, Rift Valley Fever, herpes simplex, and yellow fever.

[0216] In another specific embodiment, modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention are used as an adjuvant to enhance anti-bacterial or anti-fungal immune responses. Anti-bacterial or anti-fungal immune responses that may be enhanced using the compositions of the invention as an adjuvant, include bacteria or fungus and bacteria or fungus associated diseases or symptoms described herein or otherwise known in the art. In specific embodiments, the compositions of the invention are used as an adjuvant to enhance an immune response to a bacteria or fungus, disease, or symptom selected from the group consisting of tetanus, Diphtheria, botulism, and meningitis type B.

[0217] In another specific embodiment, the compositions of the invention are used as an adjuvant to enhance an immune response to a bacteria or fungus, disease, or symptom selected from the group consisting of Vibrio cholerae, Mycobacterium leprae, Salmonellatyphi, Salmonella paratyphi, Meisseria meningitidis, Streptococcus pneumoniae, Group B streptococcus, Shigella spp., Enterotoxigenic Escherichia coli, Enterohemorrhagic E. coli, and Borrelia burgdorferi.

[0218] In another specific embodiment, modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention are used as an adjuvant to enhance anti-parasitic immune responses. Anti-parasitic immune responses that may be enhanced using the compositions of the invention as an adjuvant, include parasite and parasite associated diseases or symptoms described herein or otherwise known in the art. In specific embodiments, the compositions of the invention are used as an adjuvant to enhance an immune response to a parasite. In another specific embodiment, the compositions of the invention are used as an, adjuvant to enhance an immune response to Plasmodium (malaria) or Leishmania.

[0219] In another specific embodiment, modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may also be employed to treat infections diseases including silicosis, sarcoidosis, and idiopathic pulmonary fibrosis; for example, by preventing the recruitment and activation of mononuclear phagocytes.

[0220] In another specific embodiment, modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention are used as an antigen for the generation of antibodies to inhibit or enhance immune mediated responses against polypeptides of the invention.

[0221] In one embodiment, modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention are administered to an animal (e.g., mouse, rat, rabbit, hamster, guinea pig, pigs, micro-pig, chicken, camel, goat, horse, cow, sheep, dog, cat non-human primate, and human, most preferably human) to boost the immune system to produce increased quantities, of one or more antibodies (e.g., IgG, IgA, IgM, and IaE), to induce higher affinity antibody production and immunoglobulin class switching (e.g., IgG, IgA, IgM, and IaE), and/or to increase an immune response.

[0222] In another embodiment, modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention are used in one or more of the applications described herein, as they may apply to veterinary medicine.

[0223] In another specific embodiment, modified transferrin fusion proteins of the invention, and/or polynucleotides encoding transferrin fusion proteins of the invention are used as a means of blocking various aspects of immune responses to foreign agents or self. Examples of diseases or conditions in which blocking of certain aspects of immune responses may be desired include autoimmune disorders such as lupus, and arthritis, as well as immunoresponsiveness to skin allergies, inflammation, bowel disease, injury, and diseases/disorders associated with pathogens.

[0224] In another specific embodiment, modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention are used as a therapy for preventing the B cell proliferation and Ig secretion associated with autoimmune diseases such as idiopathic thrombocytopenic purpura, systemic lupus erythematosus and multiple sclerosis.

[0225] In another specific embodiment, modified transferrin fusion proteins or polynucleotides encoding transferrin fusion proteins of the invention are used as an inhibitor of B and/or T cell migration in endothelial cells. This activity disrupts tissue architecture or cognate responses and is useful, for example in disrupting immune responses, and blocking sepsis.

[0226] In another specific embodiment, modified transferrin fusion proteins of the invention, and/or polynucleotides encoding transferrin fusion proteins of the invention are used as a therapy for chronic hypergammaglobulinen evident in such diseases as monoclonal gammopathy of undetermined significance (MGUS), Waldenstrom's disease, related idiopathic monocional gammopathies, and plasmacytomas.

[0227] Another specific embodiment, modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be employed for instance to inhibit polypeptide chemotaxis and activation of macrophages and their precursors, and of neutrophils, basophils, B lymphocytes and some T-cell subsets, e.g., activated and CD8 cytotoxic T cells and natural killer cells, in certain autoimmune and chronic inflammatory and infective diseases. Examples of autoimmune diseases are described herein and include multiple sclerosis, and insulin-dependent diabetes.

[0228] In another specific embodiment, modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion protein of the invention may also be employed for treating atherosclerosis, for example, by preventing monocyte infiltration in the artery wall.

[0229] In another specific embodiment, modified transferrin fusion proteins of the invention and/or -polynucleotides encoding transferrin fusion proteins of the invention may be employed to treat adult respiratory distress syndrome (ARDS).

[0230] In another specific embodiment, modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be useful for stimulating wound and tissue repair, stimulating angiogenesis, and/or stimulating the repair of vascular or lymphatic diseases or disorders. Additionally, fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be used to stimulate the regeneration of mucosal surfaces.

[0231] In a specific embodiment, modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention are used to diagnose, prognose, treat, and/or prevent a disorder characterized by primary or acquired immunodeficiency, deficient serum immunoglobulin production, recurrent infections, and/or immune system dysfunction. Moreover, modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be used to treat or prevent infections of the joints, bones, skin, and/or parotid glands, blood-borne infections (e.g., sepsis, meningitis, septic arthritis, and/or osteomyelitis), autoimmune diseases (e.g., those disclosed herein), inflammatory disorders, and malignancies, and/or any disease or disorder or condition associated with these infections, diseases, disorders and/or malignancies) including, but not limited to, CVID, other primary immune deficiencies, HIV disease, CLL, recurrent bronchitis, sinusitis, otitis media, conjunctivitis, pneumonia, hepatitis, meningitis, herpes zoster (e.g., severe herpes zoster), and/or pneumocystis carnii. Other diseases and disorders that may be prevented, diagnosed, prognosed, and/or treated with fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention include, but are not limited to, HIV infection, HTLV-BLV infection, lymphopenia, phagocyte bactericidal dysfunction anemia, thrombocytopenia, and hemoglobinuria.

[0232] In a specific embodiment, modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be used to diagnose, prognose, prevent, and/or treat cancers or neoplasms including immune cell or immune tissue-related cancers or neoplasms. Examples of cancers or neoplasms that may be prevented, diagnosed, or treated by fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention include, but are not limited to, acutemyelogenous leukemia, chronic myelogenous leukemia, Hodgkin's disease, non-Hodgkin's lymphoma, acute lymphocytic anemia (ALL) Chronic lymphocyte leukemia, plasmacytomas, multiple myetoma, Burkitt's lymphoma, EBV transformed diseases, and/or diseases and disorders described in the section entitled “Hyperproliferative Disorders” elsewhere herein.

[0233] In another specific embodiment, modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention are used as a therapy for decreasing cellular proliferation of Large B-cell Lymphomas.

[0234] In specific embodiments, the compositions of the invention are used as an agent to boost immunoresponsiveness among B cell immunodeficient individuals, such as, for example, an individual who has undergone a partial or complete splenectomy.

[0235] The modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be used to modulate homeostatic (the stopping of bleeding) or thrombolytic (clot dissolving) activity. For example, by increasing homeostatic or thrombolytic activity, fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention could be used to treat or prevent blood coagulation diseases, disorders, and/or conditions (e.g., afibrinogenemia, factor deficiencies, hemophilia), blood platelet diseases, disorders, and/or conditions (e.g. thrombocytopenia), or wounds resulting from trauma, surgery, or other causes. Alliteratively, fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention that can decrease hemostatic or thrombolytic activity could be used to inhibit or dissolve clotting. These molecules could be important in the treatment or prevention of heart attacks (infarction), strokes, or scarring.

[0236] In specific embodiments, the modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be used to prevent diagnose, prognose, and/or treat thrombosis, arteria thrombosis, venous thrombosis, thromboembolism, pulmonary embolism, atherosclerosis, myocardial infarction, transient ischenuc attack, unstable angina. In specific embodiments, the transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention maybe used for the prevention of occulsion of saphenous grafts, for reducing the risk of periprocedural thrombosis as might accompany angioplasty procedures, for reducing the risk of stroke in patients with atrial fibrillation including nonrheumatic atria fibrillation, for reducing the risk of embolism associated with mechanical heart valves and/or mitral valves disease. Other uses for the modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention, include, but are not limited to, the prevention of occlusions in extrcorporeal devices (e.g., intravascular canals, vascular access shunts in hemodialysis patients, hemodialysis machines, and cardiopulmonary bypass machines).

[0237] In another embodiment, modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention, may be used to prevent, diagnose, prognose, and/or treat diseases and disorders of the blood and/or blood forming organs associated with the tissue(s) in which the polypeptide of the invention is expressed.

[0238] The modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be used to modulate hematopoietic activity (the formation of blood cells). For example, the transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be used to increase the quantity of all or subsets of blood cells, such as, for example, erythrocytes, lymphocytes (B or T cells), myeloid cells (e.g., basophfis, eosinophtis, neutrophls, mast cells, macrophages) and platelets. The ability to decrease the quantity of blood cells or subsets of blood cells may be useful in the prevention, detection, diagnosis, and/or treatment of anemias and leukopenias described below. Alternatively, the modified transferrin fusion proteins, of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention maybe used to decrease the quantity of all or subsets of blood cells, such as, for example, erythrocytes, lymphocytes (B or T cells), myeloid cells (e.g., basophils, eosinoptifis, neutrophils, mast cells, macrophages) and platelets. The ability to decrease the quantity of blood cells or subsets of blood cells may be useful in the prevention, detection, diagnosis, and/or treatment of leukocytoses, such as, for example eosinophilia. The modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be used to prevent, treat, or diagnose blood dyscrasia.

[0239] Anemias are conditions in which the number of red blood cells or amount of hemoglobin (the protein that carries oxygen) in them is below normal. Anemia may be caused by excessive bleeding, decreased red blood cell production, or increased red blood cell destruction (hemolysis). The modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be useful in treating, preventing, and/or diagnosing anemias. Anemias that may be treated prevented or diagnosed by the transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention include iron deficiency anemia, hypochromic anemia, microcytic anemia, chlorosis, hereditary sideroblastic anemia, idiopathic acquired sideroblastic anemia, red cell aplasia, megaloblastie anemia (e.g., pemiciovis anemia, (vitamin B12 deficiency) and folic acid deficiency anemia), aplastic anemia, hemolytic anemias (e.g., autoimmune helolytic anemia, microarigiopathic hemolytic anemia, and paroxysmal nocturnal hemoglobinunia). The modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be useful in treating, preventing, and/or diagnosing anemias associated with diseases including but not limited to, anemias associated with systemic lupus erythematosus, cancers, lymphomas, chronic renal disease, and enlarged spleens. The transferrin fusion proteins of the and/or polynucleotides encoding transferrin fusion proteins of the invention may be useful in treating, preventing, and/or diagnosing anemia arising from drug treatments such as anemias associated with methyldopa, dapsone, and/or sulfa drugs. Additionally, modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention maybe useful in treating, preventing, and/or diagnosing anemias associated with abnormal red blood cell architecture including but not limited to, hereditary spherocytosis, hereditary elliptocytosis, glucose-6-phosphate dehydrogenase deficiency, and sickle cell anemia.

[0240] The modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be useful in treating, preventing, and/or diagnosing hemoglobin abnormalities, (e.g., those associated with sickle cell anemia, hemoglobin C disease, hemoglobin S-C disease, and hemoglobin E disease). Additionally, the transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be useful in diagnosing, preventing, and/or prognosing in treating thalassemias, including, but not limited to, major and minor forms of alpha-thalassemia and beta-thalassemia.

[0241] In another embodiment, the modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be useful in diagnosing, prognosing, preventing, and/or treating bleeding disorders including, but not limited to, thrombocytopenia (e.g., idiopathic thrombocytopenic purpura, and thrombotic thrombocytopenic purpura), Von Willebrand's disease, hereditary platelet disorders (e.g., storage pool disease such as Chediak-Higashi and Hermansky-Pudlak syndromes, thromboxane A2 dysfunction, thromboasthenia, and Bernard-Soulier syndrome), hemolyticuremic syndrome, hemophelias such as hemophelia A or Factor V-11 deficiency and Christmas disease or Factor IX deficiency, Hereditary Hemorhhagic Telangiectsia, also known as Rendu-Osler-Webe syndrome, allergic purpura (Henoch Schonlein purpura) and disseminated intravascular coagulation.

[0242] In other embodiments, the modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be useful as an agent to increase cytokine production.

[0243] Hyperproliferative disorders in certain embodiments, fusion proteins of the invention, and/or polynucleotides encoding transferrin fusion proteins of the invention can be used to treat or detect hyperproliferative disorders, including neoplasms. Transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may inhibit the proliferation of the disorder through direct or indirect interactions. Alliteratively, fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may proliferate other cells which can inhibit the hyperproliferative disorder.

[0244] For example, by increasing an immune response, particularly increasing antigenic qualities of the hyperproliferative disorder or by proliferating, differentiating, or mobilizing T-cells, hyperproliferative disorders can be treated. This immune response may be increased by either enhancing an existing immune response, or by initiating a new immune response. Alliteratively, decreasing an immune response may also be a method of treating hyperproliferative disorders, such as a chemotherapeutic agent.

[0245] Examples of hyperproliferative disorders that can be treated or detected by modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention include, but are not limited to neoplasms located in the: colon, abdomen, bone, breast, digestive system, liver, pancreas, peritoneum, endocrine glands (adrenal, parathyroid, pituitary, testicles, ovary, thymus, thyroid), eye, head and neck, nervous (central and peripheral), lymphatic system, pelvis, skin, soft tissue, spleen, thorax, and urogenital tract.

[0246] Similarly, other hyperproliferative disorders can also be treated or detected by modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention. Examples of such hyperproliferative disorders include, but are not limited to Acute Childhood Lymphoblastic Leukemia; Acute Lymphoblastic Leukemia, Acute Lymphocytic Leukemia, Acute Myeloid Leukemia, Adrenocortical Carcinoma, Adult (Primary) Hepatocellular Cancer, Adult (Primary) Liver Cancer, Adult Acute Lymphocytic Leukemia, Adult Acute Myeloid Leukemia, Adult Hodgkin's Disease, Adult Hodgkin's Lymphorria, Adult Lymphocytic Leukemia, Adult Non-Hodgkin's Lymphoma, Adult Primary Liver Cancer, Adult Soft Tissue Sarcoma, AIDS-Related Lymphorria, AIDS-Related Malignancies, Anal Cancer, Astrocytoma, Bile Duct Cancer, Bladder Cancer, Bone Cancer, Brain Stem Glioma, Brain Tumors, Breast Cancer, Cancer of the Renal Pelvis and Ureter, Central Nervous System (Primary) Lymphoma, Central Nervous System Lymphorria, Cerebellar Astrocytoma, Cerebral Astrocytoma, Cervical Cancer, Childhood (Primary) Hepatocellular Cancer, Childhood (Primary) Liver Cancer, Childhood Acute Lymphoblastic Leukemia, Childhood Acute Myeloid Leukemia, Childhood Brain Stem Glioma, Childhood Cerebellar Astrocytoma, Childhood Cerebral Astrocytoma, Childhood Extracranial Germ Cell Tumors, Childhood Hodgkin's Disease, Childhood Hodgkin's Lymphoma, Childhood Hypothalanic and Visual Pathway Glioma, Childhood Lymphoblastic Leukemia, Childhood Medulloblastoma, Childhood Non-Hodgkin's Lymphoma, Childhood Pineal and Supratentorial Primitive Neuroectodermal Tumors, Childhood Primary Liver Cancer, Childhood Rhabdomyosarcoma, Childhood Soft Tissue Sarcoma, Childhood Visual Pathway and Hypothalamic Glioma, Chronic Lymphocytic Leukemia, Chronic Myelogenous Leukemia, Colon Cancer, Cutaneous T-Cell Lymphoma, Endocrine Pancreas Islet Cell Carcinoma. Endometrial Cancer, Ependymoma, Epithelial Cancer, Esophageal Cancer, Ewing's Sarcoma and Related Tumors, Exocrine Pancreatic Cancer, Extraeranial Germ Cell Tumor, Extragonadal Germ Cell Tumor, Extrahepatie Bile Duct Cancer, Eye Cancer, Female Breast Cancer, Gaucher's Disease, Gallbladder Cancer, Gastric Cancer, Gastrointestinal Carcinoid Tumor, Gastrointestinal Tumors, Germ Cell Tumors, Gestational Trophoblastic Tumor, Hairy Cell Leukemia, Head and Neck Cancer, Hepatocellular Cancer, Hodgkin's Disease, Hodgkin's Lymphoma, Hypergammaglobulinemia, Hypopharyngeal Cancer, Intestinal Cancers, Intraocular Melanoma, Islet Cell Carcinoma, Islet Cell Pancreatic Cancer, Kaposi's Sarcoma, Kidney Cancer, Laryngeal Cancer, Lip and Oral Cavity Cancer, Liver Cancer, Lung Cancer, Lympho proliferative Disorders, Macroglobulinemia, Male Breast Cancer, Malignant Mesothelioma, Malignant Thymoma, Medulloblastomia, Melanoma, Mesothelioma, Metastatie Occult Primary Squamous Neck Cancer, Metastatic Primary Squamous Neck Cancer, Metastatic Squamous Neck Cancer, Multiple Mycloma, Multiple Myeloma/Plasma Cell Neoplasm, Myelodysplastic Syndrome, Myclogenous Leukemia, Myeloid Leukemia, Myeloproliferative Disorders, Nasal Cavity and Paranasal Sinus Cancer, Nasopharyrigeal Cancer, Neuroblastoma, Non-Hodgkin's Lymphoma During Pregnancy, Nonmelanoma Skin Cancer, Non-Small Cell Lung Cancer, Occult Primary Metastatic Squamous Neck Cancer, Oropharyngeal Cancer, Osteo/Malignant Fibrous Sarcoma,Osteosarcoma/Malignant Fibrous Histiocytoma, Osteosarcoma/Malignant Fibrous Histiocytoma of Bone, Ovarian Epithelial Cancer, Ovarian Germ Cell Tumor, Ovarian Low Malignant Potential Tumor, Pancreatic Cancer, Paraproteinemias, Purpura, Parathyroid, Cancer, Penile Cancer, Pheochromocytoma, Pituitary Tumor, Plasma Cell Neoplasm/Multiple Myeloma, Primary Central Nervous System Lymphoma, Primary Liver Cancer, Prostate Cancer, Rectal Cancer, Renal Cell Cancer, Renal Pelvis and Ureter Cancer, Retinoblastoma, Rhabdomyosarcoma, Salivary Gland Cancer, Sarcoidosis Sarcomas, Sezary Syndrome, Skin Cancer, Small Cell Lung Cancer, Small Intestine Cancer, Soft Tissue Sarcoma, Squamous Neck Cancer, Stomach Cancer, Supratentorial Primitive Neuroectodermal and Pineal Tumors, T-Cell Lymphoma, Testicular Cancer, Thymoma, Thyroid Cancer, Transitional Cell Cancer of the Renal Pelvis and Ureter, Transitional Renal Pelvis and Ureter Cancer, Trophoblastic Tumors, Ureter and Renal Pelvis Cell Cancer, Urethial Cancer, Uterine Cancer, Uterine Sarcoma, Vaginal Cancer, Visual Pathway and Hypothalarruc Glioma, Vulvar Cancer, Waldenstroin's Macroglobulinemia, Wilm's Tumor, and any other hyperproliferative disease, besides neoplasia, located in an organ system listed above.

[0247] In another preferred embodiment, modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention are used to, diagnose, prognose, prevent, and/or treat premalignant conditions and to prevent progression to a neoplastic or malignant state, including but not limited to those disorders described above. Such uses are indicated in conditions known or suspected of preceding progression to neoplasia or cancer, in particular, where non-neoplastic cell growth is consisting of hyperplasia, metaplasia, or most particularly, dysplasia has occurred (for review of such abnormal growth conditions, see Robbins. and Angell, 1976, Basic Pathology, 2d Ed. W. B. Saunders Co., Philadelphia, pp. 68-79).

[0248] Hyperplasia is a form of controlled cell proliferation, involving an increase in cell number in a tissue or organ, without, significant alteration in structure or function. Hyperplastic disorders which can be diagnosed, prognosed, prevented, and/or treated with fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention include, but are not limited to, angiofollicular mediastinal lymph node hyperplasia, angiolymphoid hyperplasia with eosinophilia, a typical melanocytic hyperplasia, basal cell hyperplasia, benign giant lymph node hyperplasia, cementum hyperplasia, congenital adrenal hyperplasia, congenital sebaceous hyperplasia, cystic hyperplasia, cystic hyperplasia of the breast, denture hyperplasia, ductal hyperplasia, endometrial hyperplasia, fibromuscular hyperplasia, foca epithelial hyperplasia, gingival hyperplasia, inflammatory fibrous hyperplasia, inflammatory papillary hyperplasia, intravascular papillary endothelial hyperplasia, nodular hyperplasia of prostate, nodular regenerative hyperplasia, pseudoepitheliomatous hyperplasia, senile sebaceous hyperplasia, and verrucous hyperplasia.

[0249] In another embodiment, modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention conjugated to a toxin or a radio active isotope, as described herein, may be used to treat cancers and neoplasms, including, but not limited to, those described herein. In a further preferred embodiment, transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention conjugated to a toxin or a radioactive isotope, as described herein, may be used to treat acute myelogenous leukemia.

[0250] Additionally, modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may affect apoptosis, and therefore, would be useful in treating a number of diseases associated with increased cell survival or the inhibition of apoptosis. For example, diseases associated with increased cell survival or the inhibition of apoptosis that could be diagnosed, prognosed, prevented, and/or treated by polynucleotides, polypeptides, and/or agonists or antagonists of the invention, include cancers (such as follicular-lymphomas, carcinomas with p53 mutations, and hormone-dependent tumors, including, but not limited to, colon cancer, cardiac tumors, pancreatic cancer, melanoma, retinoblastoma, glioblastoma, lung cancer, intestinal cancer, testicular cancer, stomach cancer, neuroblastoma, myxoma, inyoma, lymphoma, endothelioma, osteoblastoma, osteoclastoma, osteosarcoma, chondrosarcoma, adenoma, breast cancer, prostrate cancer, Kaposi's sarcoma and ovarian cancer); autoimmune disorders such as, multiple sclerosis, Sjogren's syndrome, Hashimoto's thyroiditis, biliary cirrhosis, Behcet's disease, Crohn's disease, polymyosifis, systemic lupus erythematosus and immune-related glomeruionephritis and rheumatoid arthritis) and viral infections (such as herpes viruses, pox viruses and adenoviruses), inflammation, graft v. host disease, acute graft rejection, and chronic graft rejection.

[0251] In preferred embodiments, modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention are used to inhibit growth, progression, and/or metastasis of cancers, in particular those listed above.

[0252] Additional diseases or conditions associated with increased cell survival that could be diagnosed, prognosed, prevented, and/or treated by modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention, include but are not limited to, progression, and/or metastases of malignancies and related disorders such as leukemia (including acute leukemia (e.g., acute lymphocytic leukemia, acute myelocytic leukemia (including myeloblastic, promyelocytic, mylomonocytic, monocytic, and erythroleukemia)) and chronic leukemia (e.g., chronic myelocytic (granulocytic) leukemia and chronic lymphocytic leukemia)), polycythemia vera, lymphomas (e.g., Hodgkin's disease and non-Hodgkin's disease), multiple mycloma, Waldenstrom's macroglobulinemia, heavy chain disease, and solid tumors including, but not limited to, Sarcomas and, carcinomas such as fibrosarcoma, myxosarcoma, fiposarcoma, chondrosarcoma, osteogenic sarcoma, chordoma, anglosarcoma, endotheliosarcoma, lymphangiosarcoma, lymphangioendotheliosarcoma, synovioma, mesothelioma, Ewing's tumor, leiomyosarcoma, rhabdomyosarcoma, colon carcinoma, pancreatic cancer, breast cancer, ovarian cancer, prostate cancer, squamous cell carcinoma, basal cell carcinoma, adenocarcinoma, sweat gland carcinoma, sebaceous gland carcinoma, papillary carcinoma, papillary adenocarcinomas, cystadenocarcinoma, medullary carcinoma, bronchogenic carcinoma, renal cell carcinoma, hepatoma, bile duct carcinoma, choriocarcinoma, seminoma, embryonal carcinoma, Wilm's tumor, cervical cancer, testicular tumor, lung carcinoma, small cell lung carcinoma, bladder carcinoma, epithelial carcinoma, glioma, astrocytoma, medulloblastoma, craniopharyngioma, ependymoma, pinealoma, emangioblastoma, acoustic neuroma, oligodendrogliomia, menangioma, melanoma, neuroblastoma, and retinoblastoma.

[0253] Diseases associated with increased apoptosis that could be diagnosed, prognosed, prevented, and/or treated by modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention, include AIDS; neurodegenerative disorders (such as Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, retinitis pigmentosa, cerebral degeneration and brain tumor or prior associated disease); autoimmune disorders (such as, multiple sclerosis, Sjogren's syndrome, Hashimoto's thyroiditis, biliary cirrhosis, Behcet's disease, Crohn's disease, polymyositis, systemiclupus erythematosus and immune-related glomerulonephritis and rheumatoid arthritis) myelodysplastic syndromes (such as a plastic anemia), graft Y host disease, ischemic injury (such as that caused by myocardial infarction, stroke and repercussion injury), liver injury (e.g., hepatitis related liver injury, ischemia/Eeperfusion injury, cholestosis (bile duct injury) and liver cancer); toxin-induced liver disease (such as that caused by alcohol), septic shock, cachexia and anorexia.

[0254] Another preferred embodiment utilizes polynucleotides encoding modified transferrin fusion proteins of the invention to inhibit aberrant cellular division, by gene therapy using the present invention, and/or protein fusions or fragments thereof.

[0255] Thus, the present invention provides a method for treating cell proliferative disorders by inserting into an abnormally proliferating cell a polynucleotide encoding modified transferrin fusion protein of the present invention, wherein said polynucleotide represses said expression.

[0256] Another embodiment of the present invention provides a method of treating cell proliferative disorders in individuals comprising administration of one or more active gene copies of the present invention to an abnormally proliferating cell or cells.

[0257] The polynucleotides of the present invention may be delivered directly to cell proliferative disorderly disease sites in internal organs, body cavities, and the like by use of imaging devices used to guide an injecting needle directly to the disease site. The polynucleotides of the present invention may also be administered to disease sites at the time of surgical intervention.

[0258] By cell proliferative disease is meant any human or animal disease or disorder, affecting any one or any combination of organs, cavities, or body parts, which is characterized by single or multiple local abnormal proliferations of cells, groups of cells, or tissues, whether benign or malignant.

[0259] Any amount of the polynucleotides of the present invention may be administered as long as it has a biologically inhibiting effect on the proliferation of the treated cells.

[0260] Moreover, it is possible to administer more than one of the polynucleotides of the present invention simultaneously to the same site. By “biologically inhibiting” is meant partial or total growth inhibition as well as decreases in the rate of proliferation or growth of the cells.

[0261] Modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention are useful in inhibiting the metastasis of proliferative cells or tissues. Inhibition may occur as a direct result of administering these transferrin fusion proteins and/or polynucleotides, or indirectly, such as activating the expression of proteins known to inhibit metastasis, for example alpha, integrins, (See, e.g., Curr Top Mirobiol Immunol 1998; 231:141, which is hereby incorporated by reference). Such therapeutic affects of the present invention may be achieved either alone, or in combination with small molecule drugs or adjutants.

[0262] In another embodiment, the invention provides a method of delivering compositions containing the transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention to targeted cells expressing the a polypeptide bound by, that binds to, or associates with a modified transferrin fusion protein of the invention. Transferrin fusion proteins of the invention may be associated with heterologous polypeptides, heterologous nucleic acids, toxins, or prodrugs via hydrophobic, hydrophilic, ionic and/or covalent interactions.

[0263] Kidney diseases which can be diagnosed, prognosed, prevented, and/or treated with compositions of the invention include, but are not limited to, acute kidney failure, chronic kidney failure, atheroembolic renal failure, end-stage renal disease, inflammatory diseases of the kidney (e.g., acute glomerulonephritis, post infectious glomerulonephritis, rapidly progressive glomerulonephritis, nephritic syndrome, membranous glomeruionephritis, familial nephritic syndrome, membrane proliferative glomerulonephritis and mesangial proliferative glomerulonephritis, chronic glomerulonephritis, acute tubulo intestinal nephritis, chronic tubulointerstitial nephritis, acute post-streptococcal glomeruionephritis(PSGN), pyelonephritis, lupus nephritis, chronic nephritis, interstitial nephritis, and post streptococcal glomerulonephritis), blood vessel disorders of the kidneys (e.g., kidney infarction, atheroembolic kidney disease, cortical necrosis, malignant nephrosclerosis, renal vein thrombosis, renal under perfusion, renal retinopathy, renal ischemia-reperfusion, renal artery embolism and renal artery stenosis), and kidney disorders resulting form urinary tract disease (e.g., pyelonephritis, hydronephrosis, urolithiasis (renal lithiasis, nephrolithiasis), reflux nephropathy, urinary tract infections, urinary retention, and acute or chronic unilateral obstructive uropathy). In addition, compositions of the invention can be used to diagnose, prognose, prevent, and/or treat metabolic and congenital disorders of the kidney (e.g., uremia, renalamyloidosis, renal osteodystrophy, renal tubular acidosis, renal glycosuria, nephrogenic diabetes insipidus, cystinuria, Fanconi's syndrome, renal fibrocystic osteosis (renal rickets), Hartnup disease, Bartter's syndrome, Liddle's syndrome, polycystic kidney disease, medullary cystic disease, medullary sponge kidney, Alport's syndrome, nail-patella syndrome, congenital nephritic syndrome, CRUSH syndrome, horseshoe kidney, diabetic nephropathy, nephrogenic diabetes insipidus, analgesic nephropathy, kidney stones, and membranous nephropathy), and autoimmune disorders of the kidney (e.g., systemic lupuserythematosus (SLE), Good pasture syndrome, IgA nephropathy, and ICFM mesangial proliferative glomerulonephritis).

[0264] Compositions of the invention can also be used to diagnose, prognose, prevent, and/or treat sclerotic or lecrotic disorders of the kidney (e.g., glomeruloselerosis, diabeticnephropathy, faca Fsegmental glomerulo sclerosis (FSGS), narcotizing glomerulonephritis, and renal papillary necrosis), cancers of the kidney (e.g., nephroma, hypemephroma, nephroblastoma, renal cell cancer, transitional cell cancer, renal adenocarcinoma, squamous cell cancer, and Wilm's tumor), and electrolyte imbalances (e.g., nephrocalcinosis, pyuria, edema, hydronephritis, proteinuria, hyponatrerrua, hypematremia, hypokalemia, hyperkalemia, hypocalcemia, hypercalcemia, hypophosphatemia, and hyperphosphatemia).

[0265] Compositions of the invention may be administered using any method known in the art, including, but not limited to, direct needle injection at the delivery site, intravenous injection, topical administration, catheter infusion, biolistic injectors, particle accelerators, gel foam sponge depots, other commercially available depot materials, osmotic pumps, oral or suppositorial solid pharmaceutical formulations, decanting or topical applications during surgery, aerosol delivery. Such methods are known in the art. Compositions of the invention may be administered as part of a Therapeutic, described in more detail below.

[0266] Modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention, may be used to treat, prevent, diagnose, and/or prognose cardiovascular disorders, including, but not limited to, peripheral artery disease, such as limb ischemia.

[0267] Cardiovascular disorders, includes, but is not limited to, cardiovascular abnormalities, such as arterio arterial fistula, arterioyenous fistula, cerebral arterioyertous malformations, congenital heart defects, pulmonary atresia, and Scimitar Syndrome.

[0268] Congenital heart defects include, but are not limited to, aortic coarctation, cortriatriatum, coronary vessel anomalies, crisscross heart, dextrocardia, patent ductus arteriosus, Ebstein's anomaly, Eisenmenger complex, hypoplastic left heart syndrome, levocardia, tetralogy of fallot, transposition of great vessels, double outlet right ventricle, tricuspidatresia, persistent truncus arteriosus, and heart septal defects, such as aortopulmonary septald defect, endocardial cushion defects, Lutembacher's Syndrome, trilogy of Fallot, ventricular heart septal defects.

[0269] Cardiovascular disorders also include, but are not limited to, heart disease, such asamhythmias, carcinoid heart disease, high cardiac output, low cardiac output, cardiactamponade, endocarditis (including bacteria), heart aneurysm, cardiac arrest, congestive heart failure, congestive cardiomyopathy, paroxysmal dyspnea, cardiac edema, heart hypertrophy, congestive cardiomyopathy left ventricular hypertrophy, right ventricularhypertrophy, post-infarction heart rupture, ventricular septal ruoture, heart valve diseases myocardial diseases, myocardial ischemia, pericardial effusion, pericarditis (including constrictive and tuberculous), pricumopericardium, post pericardiotomy syndrome, pulmonary heart disease, rheumatic heart disease, ventricular dysfunction, hyperemia, cardiovascular pregnancy complications, Scimitar Syndrome, cardiovascular syphilis, and cardiovascular tuberculosis.

[0270] Arrhythmias include, but are not limited to, sinus arrhythmia, atrial fibrillation, atrial flutter, bradycardia, extrasystole, Adams-Stokes Syndrome, bundle-branch block, sinoatrial block, long QT syndrome, parasystole, Lown-Ganong-Levine Syndrome, Mahaim-type pre-excitation syndrome, Wolff-Parkinson-White syndrome, sick sinus syndrome, itachyeardias, and ventricular fibrillation. Tachycardias include paroxysmal tachycardia, suprayentriculai tachycardia, accelerated idioventricular rhythm, atrioventricular nodal reentry tachyeardia, ectopic atrial tachycardia, ectopic junctional tachycardia, sinoattial nodalreentry tachycardia, sinus tachycardia, Torsades de Pointes, and ventricular tachycardia.

[0271] Heart valve diseases include, but are not limited to, aortic valve insufficiency aorticvalve stenosis, hear murmurs, aortic valve prolapse, neutral valve prolapse, tricuspid valve prolapse, mitral valve insufficiency, mitral valve stenosis, pulmonary atresia, pulmonary valve insufficiency, pulmonary valve stenosis, tricuspid atresia, tricuspid valve insufficiency, and tricuspid valve stenosis.

[0272] Myocardial diseases include, but are not limited to, alcoholic cardiomyopathy, congestive cardiomyopathy, hypertrophic cardiomyopathy, aortic subvalvular stenosis, pulmonary subvalvular stenosis, restrictive cardiomyopathy, Chagas cardiomyopathy, endocardial fibroelastosis, endomyocardial fibrosis, Kearns Syndrome, myocardial reperfusion injury, and myocarditis.

[0273] Myocardial schemias include, but are not limited to, coronary disease, such as angina pectoris, coronary aneurysm, coronary arteriosclerosis, coronary thrombosis, coronary vasospasm, myocardial infarction, and myocardial stunning.

[0274] Cardiovascular diseases also include vascular diseases such as aneurysms, angiodysplasia, angiomatosis, bacillary arigiomiatosis, Hippel-Lindau Disease, Klippel Trenaunay Weber Syndrome, Sturge Weber Syndrome, angioneurotic edema, aorfic diseases, Takayasu's Arthritis, aortitis, Leriche's Syndrome, arterial occlusive diseases, arthritis, enarteritis, polyarteritis nodosa, cerebrovascular disorders, diabetic angiopathies, diabetic retinopathy, embolisms, thrombosis, erythromelalgia, hemorrhoids, hepatic veno-occlusive disease, hypertension, hypotension, ischemia, peripheral vascular diseases, phlebitis, pulmonary veno-occlusive disease, Raynaud's disease, CREST syndrome, retinal vein occlusion, Scimitar syndrome, superior vena cava syndrome, telangiectasia, ataciatelangiectasia, hereditary hemorrhagic telangiectasia, varicocele, varicose veins, varicoseulcer, vasculitis, and venous insufficiency.

[0275] Cerebrovascular disorders include, but are not limited to, cardio artery diseases, Respiratory Disorders Transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be used to treat, prevent, diagnose, and/or prognose diseases and/or disorders of the respiratory system.

[0276] Diseases and disorders of the respiratory system include, but are not limited to, nasalvestibulitis, nonallergic rhinitis (e.g., acute rhinitis, chronic rhinitis, atrophic rhinitis, vasomotor rhinitis), nasal polyps, and sinusitis, juvenile angiofibromas, cancer of the nose and juvenile papillomas, vocal cord polyps, nodules (singer's nodules), contact ulcers, vocal cord paralysis, laryngoceles, pharynefitis (e.g., viral and bacterial), tonsillitis, tonsillar cellulitis, parapharyrigeal abscess, laryngitis, laryngoceles, and throat cancers (e.g., cancer of the nasopharynx, tonsil cancer, larynx cancer), lung cancer (e.g., squamous cell carcinoma, small cell (oat cell) carcinoma, large cell carcinoma, and adenocarcinoma), allergic disorders (eosinophilie pneumonia, hypersensitivity pneumonitis (e.g., extrinsicallergic alveolitis, allergic interstitial pneumonitis, organic dust pneumoconiosis, allergic bronchopulmoniary aspergillosis, asthma, Wegener's granulomatosis (granulomatousvasculifis), Goodpasture's syndrome)), pneumonia (e.g., bacterial pneumonia (e.g., Streptococcus pneumoniae (pneumoncoccal pneumonia), Staphylococcus aureus (staphylococeal pneumonia), Gram negative bacteria pneumonia (caused by, e.g., Klebuella and Pseudomas spp.), Mycoplasma pneumoniae pneumonia, Hemophilus influenza pneumonia, Legionella pneumophila (Legionnaires' disease), and Chlamydapsittaci (Psittacosis)), and viral pneumonia (e.g., influenza, chickenpox (varicella).

[0277] Additional diseases and disorders of the respiratory system include, but are not limited to bronchiolitis, polio (poliomyelitis), croup, respiratory syncytial viral infection, mumps, erythema infectiosum (fifth disease), roseola infantum, progressive rubellapanencephalitis, German measles, and subacute selerosing panencephalitis), fungal pneumonia (e.g., Histoplasmosis, Coccidioidomycosis, Blastomycosis, fungal infections in people with severely suppressed immune systems (e.g., cryptocoecosis, caused by Cryptococcus neoformans; aspergillosis, caused by Aspergillus spp.) candidiasis, caused by Candida; and mucormycosis)), Pneumocystl's carinu (pneumocystis pneumonia), a typicalpneumonias (e.g., Mycoplasma and Chlamyda spp.), opportunistic infection pneumonia, nosocomial pneumonia, chemical pneumonitis, and aspiration pneumonia, pleural disorders(e.g., pleurisy, pleural effusion, and pneumothorax (e.g., simple spontaneous pneumothorax, complicated spontaneous pneumothorax, tension pneumothorax)),obstructive airway diseases (e.g., asthma,. chronic obstructive pulmonary disease (COPID),emphysema, chronic or acute bronchitis), occupational lung diseases (e.g., silicosis, blacklung (coal workers' pneumoconiosis, asbestosis, berylliosis, occupational asthma, byssinosis, and benign pritumoconioses), Infiltrative Lung Disease (e.g., pulmonary fibrosis (e.g., fibrosincralveolifi, usual interstitial pneumonia), idiopathic pulmonary fibrosis, desquamative interstitial pneumonia, lymphoid interstitial pnetimonia, histiocytosis (e.g., Letterer-Siwe disease, Hand-Schüller-Christian disease, eoslnophific granuloma), idiopathic pulmonary hemosiderosis, sarcoidosis and pulmonary, alveolar proteinosis), Acute respiratory distress syndrome (also called, e.g., adult respiratory distress syndrome), edema, pulmonary embolism, bronchitis (e.g., viral, bacterial), bronchiectasis, atelectasis, lung abscess (caused by, e.g., Staphylococcus aureus or Legionella pneumophila), and cystic fibrosis.

[0278] Cancers which may be treated with modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention include, but are not limited to solid tumors, including prostate, lung, breast, ovarian, stomach, pancreas, larynx, esophagus, lesteg, liver, parotid, biliary tract, colon, rectuffi, cervix, uterus, lendometrium, kidney, bladder, thyroid cancer; primary tumors and metastases; melanomas; glioblastoma; Kaposi's sarcoma; leiomyosarcoma; non-small cell lung cancer; colorectal cancer; advanced malignancies; and blood born tumors such as leukemia. For example, fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be delivered topically, in order to treat cancers such as skin cancer, head and neck tumors, breast tumors, and Kaposi's sarcoma.

[0279] Modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be useful, in treating other disorders, besides cancers, which involve angiogenesis. These disorders include, but are not limited to: benigri tumors, for example hemanglomas, acoustic neuromas, neurofibromas, trachomas, and pyogenicgranulomas; artheroscleric plaques; ocular angiogenic diseases, for example, diabetic retinopathy, retinopathy of prematurity, macular degeneration, comeal graft rejection, neovascular glaucoma, retrolental fibroplasia, rubeosis, retinoblastoma, uvietis and Pterygiaab normal blood vessel growth) of the eye; rheumatoid arthritis; psoriasis; delayed wound healing; endometriosis; vasculogenesis; grantilations; hypertrophic scars (keloids); nonunion fractures; scleroderma; trachoma; vascular adhesions; myocardial angiogenesis; coronary collaterals; cerebral collaterals; arterioyenotis malformations; ischemic limb angiogenesis; Osler-Webber Syndrome; plaque neovascularization; telangiectasia; hemophiliac joints; angiofibroima; fibromuscular dysplasia; wound granulation; Crohn's disease; and atherosclerosis.

[0280] Thus, within one aspect of the present invention methods are provided for treating neovascular diseases of the eye.

[0281] Additionally, disorders which can be treated with modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention include, but are not limited to, hemangioma, arthritis, psoriasis, angiofibroma, atherosclerotic plaques, delayed wound healing, granulations hemophilic joints hypertrophic scars, nonunion fractures, Osler-Weber syndrome, pyogenic granuloma, scleroderma, trachoma; and vascular adhesions.

[0282] Moreover, disorders and/or states, which can be treated, prevented, diagnosed, and/or prognosed with the modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention include, but are not limited to, solid tumors, blood born tumors such as leukemia, tumor metastasis, Kaposi's sarcoma, benign tumors, for example hemangiomas, acoustic neuromas, neurofibromas, trachomas, and pyogenic granulomas, rheumatoid arthritis, psoriasis, ocularangiogenic diseases, for example, diabetic retinopathy, retinopathy of prematurity, macular degeneration, comeal graft rejection, neovascular glaucoma, retrolental fibroplasia, rubeosis, refinoblastoma, and uvietis, delayed wound healing, endometriosis, vascluogenesis, granulations, hypertrophic scars (keloidsj, nonunion fractures, scleroderma, trachoma, vascular adhesions, myocardial angiogenesis, coronary collaterals, cerebral collaterals, arterioyenous malformations, ischemic limb angiogenesis, Osler-Webber Syndrome, plaque neovascularization, telangiectasia, hemophiliac joints, angiofibroma fibromuscular dysplasia, wound granulation, Crohn's disease, atherosclerosis, birth control agent by preventing vascularization required for embryo, implantation controlling menstruation, diseases that have angiogenesis as a pathologic consequence such as cat scratch disease (Rochele nunalia quintosa), ulcers (Helicobacterpylori), Bartonellosis and baculary angiomatosis.

[0283] In one aspect of the birth control method, an amount of the compound sufficient to block embryo implantation is administered before or after intercourse and fertilization have occurred, thus providing an effective method of birth control, possibly a “morning after” method. Modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may also be used in controlling menstruation or administered as either a peritoneal lavage fluid or for peritoneal implantation in the treatment of endometriosis.

[0284] Modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be utilized in a wide variety of surgical procedures.

[0285] Diseases associated with increased cell survival or the inhibition of apoptosis that could be treated, prevented, diagnosed, and/or prognosed using modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention, include cancers (such as follicular lymphomas, carcinomas with mutations, and hormone-dependent tumors, including, but not limited to colon cancer, cardiac tumors, pancreatic cancer, melanoma, retinoblastoma, glioblastoma, lung cancer, intestinal cancer, testicular cancer, stomach cancer, neuroblastoma, myxoma, myoma, lymphoma, endothelioma, osteoblastoma, osteoclastoma, osteosarcoma, chondrosarcoma, adenoma, breast cancer, prostate cancer, Kaposi's sarcoma and ovarian cancer); autoimmune disorders (such as, multiple sclerosis, Sjogren's syndrome, Hashimoto's thyroiditis, biliary cirrhosis, Behcet's disease, Crohn's disease, polymyositis, systemic lupus thematosus and immune-related ryglomerulonephritis and rheumatoid arthritis) and viral infections (such as herpes viruses, pox viruses and adenoviruses), inflammation, graft v. host disease, acute graft rejection, and chronic graft rejection.

[0286] In preferred embodiments, modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention are used to inhibit growth, progression, and/or metasis of cancers, in particular those listed above.

[0287] Additional diseases or conditions associated with increased cell survival that could be treated or detected by modified fusion proteins of the invention and/or polynucleotides encoding, transferrin fusion proteins of the invention include, but are not limited to, progression, and/or metastases of malignances and related disorders such as leukemia (including acute leukemia (e.g., acute lymphocytic leukemia, acute myelocytic leukemia (including myeloblastic, promyelocytic, myelomonocytic, monocytic, and erythroleukemia)) and chronic leukemia (e.g., chronic myelocytie (granulocytic) leukemia and chroniclymphocytic leukemia)), polycytemia vera, lymphomas (e.g., Hodgkin's disease and non-Hodgkin's disease), multiple myeloma, Waldenstrorn's macroglobulinemia, heavy chain disease, and solid tumors including, but not limited to, Sarcomas and carcinomas such as fibrosarcoma, myxosarcoma, liposarcoma, chondrosarcoma, osteogenic sarcoma, chordoma, angiosarcoma, endotheliosarcoma, lymphangiosarcoma, lymphangioendotheliosarcoma, synovioma, mesothelioma, Ewing's tumor, leiomyosarcoma, rhabdomyosarcoma, colon carcinoma, pancreatic cancer, breast cancer, civarian cancer, prostate cancer, squamous cell carcinoma, basa cell carcinoma, adenocarcinoma, sweat gland carcinoma, sebaceous aland carcinoma, papillary carcinoma, papillary adenocarcinomas, cystadenocarcinoma, medullary carcinoma, bronchogeniccarcinoma, renal cell carcinoma, hepatoma, bile duct carcinoma, choriocarcinoma, seminoma, embryonal carcinoma, Wilm's tumor, cervical cancer, testicular tumor, Jung carcinoma, small cell lung carcinoma, bladder carcinoma, epithelial carcinoma, glioma, astrocytoma, medulloblastoma, craniopharyngioma, ependymoma, pinealoma, hemangioblastoma, acoustic neuroma, oligodendroglioma, menangioma, melanoma neuroblastoma, and retinoblastoma.

[0288] Diseases associated with increased apoptosis that could be treated, prevented, diagnosed, and/or prognosed using modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention, include, but are not limited to, AIDS; neurodegenerative disorders (such as Alzthmer's disease, Parkinson's disease, Amyotrophic lateral sclerosis, Retinitis pigmentosa, Cerebellar degeneration and brain tumor or prior associated disease); autoimmune disorders (such as, multiple sclerosis, Sjogren's syndrome, Hashimoto's thyroiditis, biliary cimhosis, Behcet's disease, Crohn's' disease, polymyositis, systemic lupus erythematosus and immune-related glomerulonephritis and rheumatoid arthritis) Myelodysplastic syndromes (such as aplasiic anemia), graft v. host disease, ischenuc injury (such as that caused, by myocardial infarction, stroke and reperfusion injury), liver injury (e.g., hepatitis related liver injury, ischemia/reperfusion injury, cholestosis (bile duct injury) and liver cancer); toxin-induced liver disease (such as that caused by alcohol), septic shock, cachexia and anorexia.

[0289] In addition, modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention could be, used to treat or prevent the onset of diabetes mellitus. In patients with newly diagnosed Types 1 and 11 diabetes, where some islet cell function remains, fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention, could be used to maintain the islet function so as to alleviate, delay or prevent permanent manifestation of the disease. Also, fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention could be used as an auxiliary in islet cell transplantation to improve or promote islet cell function.

[0290] The modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be used for the diagnosis and/or treatment of diseases, disorders, damage or injury of the brain and/or nervous system. Nervous system disorders that can be treated with the compositions of the invention (e.g., fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention), limited to nervous systems include, but are not limited injuries, and diseases or disorders which result in either a disconnection of axons, a diminution or degeneration of neurons, ordemyelination. Nervous system lesions which may be treated in a patient (including human and non-human mammalian patients) according to the methods of the invention, include but are not limited to, the following lesions of either the central (including spinal cord, brain) or peripheral nervous systems: (1) ischemic lesions, in which a lack of oxygen in a portion of the nervous system results in neuronal injury or death, including cerebral infarction orischemia, or spinal cord infarction or ischemia; (2) traumatic lesions, including lesions caused by physical injury or associated with surgery, for example, lesions which sever a portion of the nervous system, or compression injuries; (3) malignant lesions, in which a portion of the nervous system is destroyed or injured by malignant tissue which is either a nervous system associated malignancy or a malignancy derived from nervous system tissue; (4) infectious lesions in which a portion of the nervous system is destroyed or injured as a result of infection, for example, by an abscess or associated with infection by human immunodeficiency virus, herpes zoster, or herpes simplex virus or with Lyme disease, tuberculosis, or syphilis; (5) degenerative lesions, in which a portion of the nervous system is destroyed or injured as a result of a degenerative process including but not limited to, degeneration associated with Parkinson's disease, Alzheimer's disease, Huntington's chorea, or amyotrophic lateral sclerosis (ALS); (6) lesions associated with nutritional diseases or disorders, in which a portion of the nervous system is destroyed or injured by a nutritional disorder or disorder of metabolism including, but not limited to vitamin B 12 deficiency, folic acid deficiency, Wemicke disease, tobacco-alcohol amblyopic, Marchiafava-Blanami disease (primary degeneration of the corpus callosum), and alcoholic cerebral degeneration; (7) neurological lesions associated with systemic diseases including, but not limited to diabetes (diabetic neuropathy, Bell's palsy), systemic lupuserythematosus, carcinoma, or sarcoidoisis; (8) lesions caused by toxic substances including alcohol, lead, or particular, neurotoxins; and (9) demyelinated lesions in which a portion of the nervous system is destroyed or injured by a demyelinating disease including, but not limited to, multiple sclerosis, human immunodeficiency virus-associated myelopathy, transverse myclopathy or various etiologies, progressive multifocal leukoencephalopathy, and central pontine myelinolysis.

[0291] In one embodiment, the modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention are used to protect neural cells from the damaging effects of hypoxia. In a further preferred embodiment, the modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention are used to protect neural cells from the damaging effects of cerebral hypoxia.

[0292] In specific embodiments, motor neuron disorders that may be treated according to the invention include, but are not limited to, disorders such as infarction, infection, exposure to toxin, trauma, surgical damage, degenerative disease or malignancy that may affect motor neurons as well as other components of the nervous system, as well as disorders that selectively affect neurons such as amyotrophic lateral sclerosis, and including, but not limited to, progressive spinal muscular atrophy, progressive bulbar palsy, primary lateral sclerosis, infantile and juvenile muscular atrophy, progressive bulbar paralysis of childhood (Fazio-Londe syndrome), poliomyelitis and the post polio syndrome, and Hereditary Motor sensory Neuropathy (Charcot-Marie-Tooth Disease).

[0293] Further, modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may play a role in neuronal survival; synapse formation; conductance; neural differentiation, etc. Thus, compositions of the invention (including fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention) may be used to diagnose and/or treat or prevent diseases or disorders associated with these roles, including, but not limited to, learning and/or cognition disorders. The compositions of the invention may also be useful in the treatment or prevention of neurodegenerative disease states and/or behavioral disorders. Such neurodegenerative disease states and/or behavioral disorders include, but are not limited to, Alzheimer's Disease, Parkinson's Disease, Huntington's Disease, Tourette Syndrome, schizophrenia, mania, dementia, paranoia, obsessive compulsive disorder, panic disorder, learning disabilities, ALS, psychoses, autism, and altered behaviors, including disorders in feeding, sleep patterns, balance, and perception.

[0294] Examples of neurologic diseases which can be treated or detected with modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention include, brain diseases, such as metabolic brain diseases which includes phenylketonuria such as maternal phenylketonuria, pyruvate carboxylase deficiency, pyruyate dehydrogenase complex deficiency, Wernicke's Encephalopathy, brain edema, brain neoplasms such as cerebellar neoplasms which include infratentorial neoplasms, cerebral ventricle neoplasms such as chorod plexus neoplasms, hypothalanic necoplasms, supratentorial neoplasms, canavan disease, cerebellar diseases such as cerebellar ataxia which include spinocerebellar degeneration such as ataxia telangiectasia, cerebellar dyssynergia, Friederich's Ataxia, Machado-Joseph Disease, olivopontocerebellar atrophy, cerebellar neoplasms such as infratentorial neoplasms, diffuse cerebral sclerosis such asencephalitis periaxialis, globoid cell leukodystrophy, metachromatic leukodystrophy and subacute sclerosing panencephalitis.

[0295] Additional neurologic diseases which can be treated or detected with modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention include cerebrovascular disorders (such as carotid artery diseases which include carotid artery thrombosis, carotid stenosis and Moyamoya Disease), cerebral amyloid angiopathy, cerebral aneurysm, cerebral anoxia, cerebral arteriosclerosis, cerebral arterioyenous malformations, cerebral artery diseases, cerebral embolism and thrombosis such as carotid artery thrombosis, sinus thrombosis and Wallenberg's Syndrome, cerebral hemorrhage such as epidermal hematoma, subdural hematoma and subarachnoid hemorrhage, cerebral infarction, cerebral ischemia such as transient cerebral ischemia, Subclavian Steal Syndrome and vertebrobasilar insufficiency, vascular dementia such as multi-infarct dementia, periventricular leukomalacia, vascular headache such as cluster headache and migraine.

[0296] Additional neurologic diseases which can be treated or detected with modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention include dementia such as AIDS Dementia Complex, presenile dementia such as Alzheimer's Disease and Creutzféldt-Jakob Syndrome, senile dementia such as Alzheimer's Disease and progressive supranuclear palsy, vascular dementia such as multi-infarct dementia, encephalitis which include encephalitis periaxialis, viral encephalitis such as epidemicencephalitis, Japanese Encephalitis, St. Louis Encephalitis, tick-borne encephalitis and West Nile Fever, acute disseminated encephalomyelitis, meningoencephalitis such as uveomeningoencephalitic syndrome, Postencephalitic Parkinson Disease and subacute sclerosing panencephalitis, encephalomalacia such as periventricular lieukomalacia, epilepsy such as generalized epilepsy, which includes infantile spasms, absence epilepsy, myoclonic epilepsy which includes MERRF Syndrome, tonic-clonic epilepsy, partial epilepsy such as complex partial epilepsy, frontal lobe epilepsy and temporal lobe epilepsy, post-traumatic epilepsy, status epilepticus such as Epilepsia Partialis Continua, and Hallervorden-Spatz Syndrome.

[0297] Additional neurologic diseases which can be treated or detected with modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention include hydrocephalus such as Dandy-Walker Syndrome and normal pressure hydrocephalus, hypothalamic diseases such as hypothalamic neoplasms, cerebral malaria, narcolepsy which includes cataplexy, bulbar poliomyelitis, cerebripseudo tumor, Rett Syndrome, Reye's Syndrome, thalamic diseases, cerebral toxoplasmosis, intracranialtuberculoma and Zellweger Syndrome, central nervous system infections such as AIDS, Dementia Complex, Brain Abscess, subdural empyema, encephalomyelitis such as Equine Encephalomyelitis, Venezuelan Equine Encephalomyelitis, Necrotizing Hemorrhabaic Encephalomyelitis, Visna, and cerebral malaria.

[0298] Additional neurologic diseases which can be treated or detected with modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention include meningitis such as araclinoiditis, aseptic meningitis such as viral meningitis which includes lymphocytic chronic meningitis, Bacterial meningitis which includes Haemophilus Meningitis, Listeria Meningitis, Meningococcal Meningitis such as Waterhouse-Fridericlisen Syndrome, Pneumococcal Meningitis and meningeal tuberculosis, fungal meningitis such as Cryptococcal Meningitis, subdural effusion, meniapencephalitis such as uvemenineroencephalitic syndrome, myelitis such as transverse myelitis, neurosyphilis such as tabes dorsalis, poliomyelitis which includes bulbar poliomyelifis and post poliomyelitis syndrome, prion diseases (such as Creutzfeldt-Jakob Syndrome, Bovine Spongiform Encephalopathy, Gerstmann-Straussler Syndrome, Kuru, Scrapie), and cerebral toxoplasmosis.

[0299] Additional neurologic diseases which can be treated or detected with modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention include central nervous system neoplasms such as brain neoplasms that include cerebellameoplasms such as infratentorial neoplasms, cerebral ventricle neoplasms such as choroidplexus neoplasms, hypothalamic neoplasms and supratentorial neoplasms, meningealneoplasms, spinal cord neoplasms which include epidural neoplasms, demyelinating diseases such as Canavan Diseases, diffuse cerebral sculleries which include sadrenoleukodystrophy, encephalifis periaxialis, globoid cell leukodystrophy, diffuse cerebral sclerosis such as metachromatic leukodystrophy, allergic encephalomyelitis, necrotizina hemorrhagic encephalomyelitis, progressive multifocal leukoencephalopathy, in multiple sclerosis, central pontine iriyelinolysis, transverse myelitis, neuroinyelitis optica, Scrapie, Swayback, Chronic Fatigue Syndrome, Visna, High Pressure Nervous Syndrome, Meningism, spinal cord diseases such as arriyotonia congenita, amyotrophic lateral-sclerosis, spinal muscular atrophy such as Werdnig-Hoffmann Disease, spinal cord compression, spinal cord neoplasms such as epidural neoplasms, syringomyelia., Tabes Dorsalis, Stiff-Man Syndrome, mental retardation such as Angelman Syndrome, Cri-du-Chat Syndrome, De Lange's Syndrome, Down Syndrome, Gangliosidoses such as gangliosidoses G(MI), Sandhoff Disease, Tay-Sachs Disease, Hartnup Disease, homocystinuria, Laurence-Moon-Bied Syndrome, Lesch-Nylian Syndrome, Maple Syrup Urine Disease, mucolipidosis such as fucosidosis, neuronal ceroid-fipofuscinosis, oculocerebrorenal syndrome, phenylketonuria such as maternal phenylketonuria, Prader-Willi Syndrome, Rett Syndrome, Rubinstein-Taybi Syndrome, Tuberous Sclerosis, WAGR Syndrome, nervous system abnormalities such as holoprosencephaly, neural tube defects such as anencephaly which includes hydrangencephaly, Arnold-Chairi Deformity, encephalocele, meningocele, meningomyelocele, spinal dysraphism such as Spina bifidacystica and spina bifida occulta.

[0300] Endocrine system and/or hormone imbalance and/or diseases encompass disorders of uterine motility including, but not limited to complications with pregnancy and labor (e.g., pre-term labor, post-term pregnancy, spontaneous abortion, and slow or stopped labor); and disorders and/or diseases of the menstrual cycle, (e.g., dysmenorrhea and endometriosis).

[0301] Endocrine system and/or hormone imbalance disorders and/or diseases include disorders and/or diseases of the pancreas, such as, for example, diabetes mellitus, diabetes insipidus, congenital pancreatic agenesis, pheochromocytoma islet cell tumor syndrome; disorders and/or diseases of the adrenal glands such as, for example, Addison's Disease, corticosteroid deficiency, virilizing disease, hirsutism, Cushing's Syndrome, hyperaldosterlonism, pheochromocytoma; disorders and/or diseases of the pituitary gland, such as, for example, hyperpituitarism, hypopituitarism, pituitary dwarfism, pituitaryadenoma, panhypopituitarism, acromegaly, gigantism; disorders and/or diseases of the thyroid, including but not limited to, hyperthyroidism, hypothyroidism, Plurnrner's disease, Graves' disease (toxic diffuse goiter), toxic nodular goiter, thyroiditis (Hashimoto's thyroiditis, subacute granulomatous thyroiditis, and silent lymphocytic thyroiditis), PendreWs syndrome, myxedema, cretinism, thyrotoxicosis, thyroid hormone coupling defect, thymic aplasia, Hurthle cell tumors of the thyroid, thyroid cancer, thyroid carcinoma, Medullary thyroid carcinoma; disorders and/or diseases of the parathyroid, such as, for example, hyperparathyroidism, hypoparathyroidism; disorders and/or diseases of the hypothalamus.

[0302] In addition, endocrine system and/or hormone imbalance disorders and/or diseases may also include disorders and/or diseases of the testes or ovaries, including cancer. Other disorders and/or diseases of the testes or ovaries further include, for example, ovarian cancer, polycystic ovary syndrome, Klinefelter's syndrome, vanishing testes syndrome (bilateral anorchia), congenital absence of Leydig's cells, cryptorchidism, Noonan's syndrome, myotonic dystrophy, capillary haemangioma of the testis (benign), neoplasias of the testis and neotestis.

[0303] Moreover, endocrine system and/or hormone imbalance disorders and/or diseases may also include disorders and/or diseases such as, for example, polyglandular deficiency syndromes, pheochromocytoma, neuroblastoma, multiple Endocrine neoplasia, and disorders and/or cancers of endocrine tissues.

[0304] The modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be used for the diagnosis, treatment, or prevention of diseases and/or disorders of the reproductive system. Reproductive system disorders that can be treated by the compositions of the invention, include, but are not limited to, reproductive system injuries, infections, neoplastic disorders, congenital defects, and diseases or disorders will result in infertility, complications with pregnancy, labor, or parturition, and postpartum difficulties.

[0305] Reproductive system disorders and/or diseases include diseases and/or disorders, of the testes, including testicular atrophy, testicular feminization, cryptorchism (unilateral and. bilateral), anorchia, ectopic testis, epididymitis and orchitis (typically resulting from infections such as, for example, gonorrhea, mumps, tuberculosis, and syphilis), testiculartorsiori, vasitis nodosa, germ cell tumors (e.g., seminomas, embryonal cell carcinomas, teratocarcinomas, choriocarcinomas, yolk sac tumors, and teratomas), stromal tumors (e.g., Leydig cell tumors), hydrocele, hematocele, varicocele, spermatocele, inguinal hemia, and disorders of sperm production (e.g. immotilc cilia syndrome, spermia, asthenozoospermia, azoospermia, oligospermia, and teratozoospermia).

[0306] Reproductive system disorders also include disorders of the prostate gland, such as acute non-bacterial rostatitis, chronic non-bacterial prostatitis, acute bacterial prostatitis, chronic bacterial prostatitis, postatodystonia, prostatosis, granulomatotis prostatitis, malacoplakia, benign prostatic hypertrophy or hyperplasia, and prostate neoplastic disorders, including adenocarcinomas, transitional cell carcinomas, ductal carcinomas, and squamous cell carcinomas.

[0307] Additionally, the compositions of the invention may be useful in the diagnosis, treatment, and/or prevention of disorders or diseases of the penis and urethra, including inflammatory disorders, such as balanoposthitis, balanitis xerotica obliterans, phimosis, paraphmosis, syphilis, herpes simplex virus, gonorrhea, non-gonococcal urethritis, clilamydia, ruycoplasma, trichomonas, HIV, AIDS, Reiter's syndrome, condylomaacuminatum, condyloma latum, and pearly penile papules, urethral abnormalities, such as hypospadias, epispadias, and phimosis, premalignant lesions, including Erythroplasia of Queyrat, Bowen's disease, Bowenoid paplosis, criant condyloma of Buscke-Lowenstein, and varrucous carcinoma; penile cancers, including squamous cell carcinomas, carcinoma in situ, verrucous carcinoma, and disseminated penile carcinoma; urethral neoplastic disorders, including penile urethial carcinoma, bulbomembranotis urethial carcinoma, and prostaticurethral carcinoma; and erectile disorders, such as priapism, Peyronie's disease, erectile dysfunction, and impotence.

[0308] Moreover, diseases and/or disorders of the vas deferens include vasculititis and CBAVD (congenital bilateral absence of the vas deferens); additionally, the transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be used in the diagnosis, treatment, and/or prevention of diseases and/or disorders of the seminal vesicles, including hydatid disease, congenital chloride diarrhea, and polycystic kidney disease.

[0309] Other disorders and/or diseases of the male reproductive system include, for example, Klinefelters syndrome, Young's syndrome, premature ejaculation, diabetes mellitus, cystic fibrosis, Kartagener's syndrome, high fever, multiple sclerosis, and gynecomastia.

[0310] Further, the polynuclotides, modified fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be used in the diagnosis treatment and/or prevention of diseases and/or disorders of the vagina and vulva, including bacterial vaginosis, candida vaginitis, herpes simplex virus, chancroid, granuloma inguinale, lymphogranuloma venereum, scabies, human papillomavirus, vaginal trauma, vulvartrauma, adenosis, chIamydia vaginitis, gonorrhea, trichomonas vaginitis, condylomaacuminatum, syphilis, molluscum contagiosum, atrophic vaginitis, Paaet's disease, lichensclerosus, lichen planus, vulvodynia, toxic shock syndrome, vaginismus, vulvovaginitis, vulvar vestibulitis, and neoplastic disorders, such as squamous cell hyperplasia, clear cellcarcinoma, basal cell carcinoma, melanomas, cancer of Bartholin's gland, and vulvarintraepaelial neoplasia.

[0311] Disorders and/or diseases of the uterus include dysmenorrhea, retroverted uterus, endometriosis, fibroids, adenomyosis, anovulatory bleeding, amenorrhea, Cushiner's syndrome, hydatidiform moles, Asherman's syndrome, premature menopause, precocious puberty, uterine polyps, dysfunctional uterine bleeding (e.g., due to aberrant hormonal signals), and neoplastic disorders, such as adenocarcinomas, keiomyosarcomas, and sarcomas. Additionally, the transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may be useful as a marker or detector of, as well as, in the diagnosis, treatment, and/or prevention of congenital uterine abnormalities, such as bicomuate uterus, septate uterus, simple unicomuate uterus, unicomuate uterus with a noncavitary rudimentary horn, unicorriuate uterus with a non-communicating cavitary rudimentary horn, unicomuate uterus with a communicating cavitary horn, arcuate uterus, uterine didelfus, and T-shaped uterus.

[0312] Ovarian diseases and/or disorders include an ovulation, polycystic ovary syndrome (Stein-Leventhal syndrome), ovarian cysts, ovarian hypofunction, ovarian insensitivity to gonadotropins, ovarian over production of androgens, right ovarian vein syndrome, in amenorrhea, hirutism, and ovarian cancer (including, but not limited to, primary and secondary cancerous growth, Sertoli-Leydig tumors, endometriod carcinoma of the ovary, ovarian papillary serous adenocarcinoma, ovarian mucinous adenocarcinoma, and Ovarian Krukenberg tumors).

[0313] Cervical diseases and/or disorders include cervicitis, chronic cervicitis, mucopurulent cervicitis, and cervical. dysplasia, cervical polyps, Nabothian cysts, cervical erosion, cervical incompetence, and cervical neoplasms (including, for example, cervical carcinoma, squamous metaplasia, squamous cell carcinoma, adenosquamous cell neoplasia, and columnar cell neoplasia).

[0314] Modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention can be used to treat or detect infectious agents. For example, by increasing the immune response, particularly increasing the proliferation and differentiation of B and/or T cells, infectious diseases may be treated. The immune response may be increased by either enhancing an existing immune response, or by fusion proteins of the invention and/or initiating a new immune response. Alternatively, polynucleotides encoding transferrin fusion proteins of the invention may also directly inhibit infectious agent, without necessarily eliciting an immune response.

[0315] Viruses are one example of an infectious agent that can cause disease or symptoms that can be treated or detected by transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention. Examples of viruses, include, but are not limited to the following DNA and RNA viruses and viral families: Arbovirus, Adenoviridae, Arenaviridae, Arterivirus, Bimaviridae, Bunyaviridae, Caliciviridae, Circoviridae, Coronaviridae, Dengue, EBV, HIV, Flaviviridae, Hepadnaviridae Hepatitis, Herpesviridae (such as, Cytomegalovirus, Herpes Simplex, Berpes Zoster), Mononegavirus (e.g., Paramyxoviridae, Morbillivirus, Rhabdoviridae), Orthomyxoviridae (e.g., Influenza A, Influenza B, and parainfluenza), Papiloma virus, Papovaviridae, Parvoviridae, Picomaviridae, Poxyiridae (such as Smallpox or Vaccinia), Reoviridae (e.g., Rotavirus), Retroviridae (HTLV-1, HTLV-11, -Lentivirus), and Togaviridae (e.g., Rubivirus).

[0316] Similarly, bacterial and fungal agents that can cause disease or symptoms that can be treated or detected by transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention include, but not limited to, the following Gram-negative and Gram-positive bacteria, bacterial families, and fungi: Actinomyces (e.g., Norcardia), Acinetobacter, Cryptococcus neoformans, Aspergillus, Bacillaceae (e.g., Bacillus anthrasis), Bacteroides (e.g., Bacteroides fragilis), Blastomycosis, Bordetella, Borrelia (e.g., Borrelia burgdorferi), Brucella, Candidia, Campylobacter, Chiamydia, Clostridiuffi (e.g., Clostridium botulinum, Clostridium dificile, Clostridium perfringens, Clostridiumtetani), Coccidioides, Corynebacterium (e.g., Corynebacterium-diptheriae), Cryptococcus, Dermatocycoses, E. coli (e.g., Enterotoxigenic E. coli and Enterohemorrhagic E. coli), Enterobacter (e.g. Enterobacter aerogenes), Enterobacteriaceac (Klebsiella, Salmonella (e.g., Salmonella typhi, Salmonella enteritidis, Salmonella typhi), Serratia, Yersinia, Shigella), Erysipelothrix, Haemophilus (e.g., Haemophilus influenza type B), Helicobacter, Legionella (e.g., Legionella pneumophila), Leptospira, Listeria (e.g., Listeria monocytogenes), Mycoplasma, Mycobacterium (e.g., Mycobacterium, leprae and Mycobacterium tuberculosis), Vibrio (e.g., Vibrio cholerae), Neisseriaceae (e.g., Neisseriagonorrhea, Neisseria meningitidis), Pasteurellacea, Proteus, Pseudomonas (e.g., Pseudomionas aeruginosa), Rickettsiaceae, Spirochetes (e.g., Treponema. spp., Leptospiraspp., Borrielia spp.), Shigella spp., Staphylococcus (e.g., Staphylococcttaureus), Meningiococcus, Pneumococeus and Streptococeus (e.g., Streptococeus pneumoniae and Groups A, B, and C Streptococci), and Ureaplasmas.

[0317] Moreover, parasitic agents causing disease or that can be treated, prevented, and/or diagnosed by fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention include, but not limited to, the following families or class: Amebiasis, Babesiosis, Coccidiosis, Cryptosporidiosis, Dientamoebiasis, Dourine, Ectoparasitic, Giardias, Helminthiasis, Leishmaniasis, Schistisoma, Theileriasis, Toxoplasmosis, Trypanosomiasis, and Trichomonas and Sporozoans (e.g., Plasmodiumvirax, Plasmodium falciparium, Plasmodium malariae and Plasmodium ovale).

[0318] Modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention can be used to differentiate, proliferate, and attract cells, pleading to the regeneration of tissues. (See, Science 276:59-87 (1997)). The regeneration of tissues could be used to repair, replace, or protect tissue damaged by congenital defects, trauma (wounds, bums, incisions, or ulcers), age, disease (e.g. osteoporosis, osteocarthritis, periodontal disease, liver failure), surgery, including cosmetic plastic surgery, fibrosis, reperfusion injury, or systemic cytokine damage.

[0319] Tissues that could be regenerated using the present invention include organs (e.g., pancreas, liver, intestine, kidney, skin, endothelium), muscle (smooth, skeletal or cardiac), vasculature (including vascular and lymphatics), nervous, hematopoietic, and skeletal (bone, cartilage, tendon, and ligament) tissue. Preferably, regeneration occurs without or decreased scarrina. Regeneration also may include angiogenesis.

[0320] Modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention, may be used to treat, prevent, diagnose, and/or prognose gastrointestinal disorders, including inflammatory diseases and/or conditions, infections, cancers (e.g., intestinal neoplasms (carcinoid tumor of the small intestine, non-Hodgkin's lymphoma of the small intestine, small bowilymphoma), and ulcers, such as peptic ulcers.

[0321] Gastrointestinal disorders include dysphagia, odynophagia, inflammation of the esophagus, peptic esophagitis, gastric reflux, submucosal fibrosis and structuring, Mallory-Weiss lesions, leioinyomas, lipomas, epidennal cancers, adeoncarcinomas, gastric retention disorders, gastroenteritis, gastric atrophy, gastric/stomach cancers, polyps of the stomach, autoimmune disorders such as pernicious anemia, pyloric stenosis, gastritis (bacterial, viral, eosinophilic, stress-induced, chronic erosive, atrophic, plasma cell, and Menetrier's), and peritoneal diseases (e.g., chylo perioneum, hemoperitoneum, mesenteric cyst, mesentericlymphadenitis, mesentenic vascular occlusion, panniculiti, neoplasins, peritonitis, prieumoperitoneum, bubphrenic abscess.

[0322] Gastrointestinal disorders also include disorders associated with the small intestine, such as malabsorption syndrome's, distension, irritable bowel syndrome, sugar idolerance, celiac disease, duodenal ulcers, duodenitis, tropical sprue, Whipple's disease, intestinal lymphangiectasia, Crohn's disease, appendicitis, obstructions of the ilum, Meckel's diverticulum, multiple diverticula, failure of complete rotation of the small and large intestine, lymphoma, and bacterial and parasitic diseases (such as Traveler's diarrhea, typhoid and paratyphoid, cholera, infection by Roundworms (Ascariasis Itimbricoides), Hookworms (Anclostoma duodenale), Threadworms (Enterobius vermicularis), Tapeworms jaenia saginata, Echinococcus granulosus, Diphyllobothrium spp. and T. SOHUM).

[0323] Liver diseases and/or disorders include intrahepatic cholestasis (alagille syndrome, biliary liver cirrhosis), fatty, liver (alcoholic fatty liver, reye syndrome), hepatic veiri, thrombosis, hepatolentricular degeneration, hepatomegaly, hepatopulmonary syndrome, hepatorenal, syndrome, portal hypertension (esophageal and gastric varices), liver abscess (amebie liver abscess), liver cirrhosis (alcoholic, biliary and experimental), alcoholic liver diseases (fatty liver, hepatitis, cirrhosis), parasitic (hepatic echinocoecosis, fascioliasis, amebic liver abscess), jaundice (hemolytic, hepatocellular, and cholestatic), cholestasis, portal hypertension, liver, enlargement, ascites, hepatitis (alcoholic hepatitis, aniffial hepatitis, chronic hepatitis (autoimmune, hepatitis B, hepatitis C, hepatitis D, drug induced), toxic hepatitis, viral human hepatitis (hepatitis A, hepatitis B, hepatitis C, hepatitis D, hepatitis E), Wilson's disease, granulomatous hepatitis, secondary biliary cirrhosis, hepaticencephalopathy, portal hypertension, varices, hepatic encepbalopathy, primary biliary heinarigiomas, bilecirrhosis, primary sclerosing cholangitis, hepatocellular adenoma, stones, liver failure (hepatic encephalopathy, acute liver failure), and liver neoplasins (ancriomyolipoma, calcified liver metastases, cystic liver metastases, epithelial tumors, fibro lamellar hepatocarcinoma, focal nodular hyperplasia, hepafic adenoma, hepatobiliarycystadenoma, hepatoblastorria, hepatocellular carcinoma, hepatoma, liver cancer, liver hemanaioendothelioma, mesenchymal hamartoma, mesenchymal tumors of liver, nodular regenerative hyperplasia, benign liver tumors (Hepatic cysts, Simple cysts, Polycystic liver disease, Hepatobiliary cystadenoma, Chofedochal cysts, Mesenchymal tumors, Mesenchymal hamartoma, Infantile hemarigioendothelioma, Hemangioma, Peliosis hepatis, Lipomas, Inflammatory pseudo tumor, Miscellaneous Epithelial tumors, Bile ductepitheflum (Bile duct hamartoma, Bile duct adenoma), Hepatocyte (Adenoma, Focal nodular hyperplasia, Nodular regenerative hyperplasia), malignant liver tumors (hepatocellular, hepatoblastoma, hepatocellular carcinoma, cholangiocellular, cholangiocarcinoma, cystadenocarcinoma, tumors of blood vessels, anaiosarcoma, Karposi's sarcoma, hemangioendothelioma, other tumors, embryonal sarcorria, fibrosarcoma, Ieiorriyosarcoma, rhabdomyosarcoma, carcinosarcoma, teratoma, carcinoid, squamous carcinoma, primarylymphorria)), peliosis hepatis, erythrohepatic porphyria, hepatic porphyria (acute interirtittentporphyria, porphyria cutanea tarda), Zelli Neger syndrome).

[0324] Pancreatic diseases and/or disorders include acute pancreatitis, chronic pancreatitis (acute necrotizing pancreatitis, alcoholic pancreatitis), neoplasins (adenocarcinoma of the pancreas, cystadenocarcinoma, insulinoma, gastrinoma, and glucacronoma, cysticcitmeoplasms, islet-cell tumors, pancreoblastoma), and other pancreatic diseases (e.g., cysticfibrosis, cyst (pancreatic pseudocyst, pancreatic fistula, insufficiency)).

[0325] Gallbladder diseases include gallstones (cholelithiasis and choledocholithiasis), postcholeeystectomy syndrome, diverticulosis of the gallbladder, acute cholecystitis, chronic cholecystitis, bile duct tumors, and mucocele.

[0326] Diseases and/or disorders of the large intestine include antibiotic-associated colitis, diverticulitis, ulcerative colitis, acquired megacolon, abscesses, fungal and bacterial infections, anorectal disorders (e.g., fissures, hemorrhoids), colonic diseases (colitis, colonic neoplastris, colon cancer, adenomatous colon polyps (e.g., villous adenoma), coloncarcinoma, colorectal cancer, colonic diverticulitis, colonic diverticulosis, megacolon, Hirschsprung disease, toxic inegacolon, sigmoid diseases proctocolitis, sigmoinneoplasmsj, constipation, Crohn's disease, diarrhea (infantile diarrhea, dysentery), duodenal diseases (duodenal neoplasins, duodenal obstruction, duodenal ulcer, duodenitis), enteritis (enterocolitis), HIV enteropathy, teal diseases (leal neoplasins, ileitis), immunoproliferative small intestinal disease, inflammatory bowel disease (ulcerative colitis, Crohn's disease), intestinal atresia, parasitic diseases (anisakiasis, balantidiasis, blastocystis infections, cryptosporidiosis, dientamoebiasis, amebic dysentery, giardiasis), intestinal fistula (rectal fistula), intestinal neoplasms (cecal neoplasms, colonic neolasms, duodenalpneoplasms, teal neoplasms, intestinal polyps, jejunal neoplasins, rectal neoplasms), intestinal obstruction (afferent loop syndrome, duodenal obstruction, impacted feces, intestinal pseudo obstruction cecal volvulus, intussusception), intestinal perforation, intestinal polyps (colonic polyps, gardner syndrome, peutz-jeghers syndrome), jejunal diseases Oejunal neoplasms), mal absorption syndromes (blind loop syndrome, celiac disease, lactose intolerance, short bowl syndrome, tropical sprue, whipple's disease), mesenteric vascular occlusion, pneumatosis cystoides intestinalis, protein losing enteropathies (intestinal lymphagiectasis), rectal diseases (anus diseases, fecal incontinence, hemorrhoids, proctitis, rectal fistula, rectal prolapse, rectocele), peptic ulcer (duodenalulcer, peptic esophagitis, hemorrhage, perforation, stomach ulcer, Zollinger-Ellison syndrome), postgastrectomy syndromes (dumping syndrome), stomach diseases (e.g., achlorhydria, duodenogastric reflux (bile reflux), gastric antral vascular ectasia, gastricfistula, gastric outlet obstruction, gastritis (atrophic or hypertrophic), gastroparesis, stomach dilatation, stomach diverticulum, stomach neoplasms (gastric cancer, gastric polyps, gastric adenocarcinoma, hyperplastic gastric polyp), stomach rupture, stomach ulcer, stomach volvulus), tuberculosis, visceroptosis, vomiting (e.g., hematemesis, hyperemesisgravidarum, postoperative nausea-and vomiting) and hemorrhagic colitis.

[0327] Further diseases and/or disorders of the gastrointestinal system include biliary tract diseases, such as, gastroschisis, fistula (e.g., biliary fistula, esophageal fistula, gastricfistula, intestinal fistula, pancreatic fistula), neoplasms (e.g., biliary tract neoplasins, esophageal neoplasms, such as adenocarcinoma of the esophagus, esophageal squamous cell carcinoma, gastrointestinal neoplasms, pancreatic neoplasins, such as adenocarcinoma of the pancreas, mucinous cystic neoplasm of the pancreas, pancreatic cystic neoplasms, pancreatoblastoma, and peritoneal neoplasms), esophageal disease (e.g., bullous diseases, candidiasis, glycoaenie acanthosis, ulceration, barrett esophagus varices, atresia, cyst, diverticulum. (e.g., Zenker's diverticulum), fistula (e.g., tracheoesophageal fistula), motility disorders (e.g., CREST syndrome, deglutition disorders, achalasia, spasm, gastroesophageal reflux), neoplasms, perforation (e.g., Boerhaave syndrome, Mallory-Weiss syndrome), stenosis, esophagitis, diaphragmatic hernia (e.g., hiatal hernia); gastrointestinal diseases, such as, gastroenteritis (e.g., cholera morbus, norwalk virus infection), hemorrhage (e.g., hematemesis, melena, peptic ulcer hemorrhage), stomach neoplasms (gastric cancer, gastric polyps, gastric adenocarcinoma, stomach cancer)), hernia (e.g., congenital diaphragmatic hernia, femoral hernia, inguinal hernia, obturator hernia, umbilical hernia, ventral hernia), and intestinal diseases (e.g., cecal diseases (appendicitis, cecal neoplasms)).

[0328] Modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may have chemotaxis activity. A chemotaxic molecule attracts or mobilizes cells (e.g., monocytes, fibroblasts, neutrophils, T-cells, mast cells, eosinophils, epithelial and/or endothelial cells) to a particular site in the body, such as inflammation, infection, or site of hyperproliferation. The mobilized cells can then fight off and/or heal the particular trauma or abnormality.

[0329] Modified transferrin fusion proteins of the invention and/or polynucleotides encoding transferrin fusion proteins of the invention may increase chemotaxic activity of particular cells. These chemotactic molecules can then be used to treat inflammation, infection, hyperproliferative disorders, or any immune system disorder by increasing the number of cells targeted to a particular location in the body.

[0330] Transgenic Animals

[0331] The production of transgenic non-human animals that contain a modified transferrin fusion construct with increased serum half-life increased serum stability or increased bioavailability of the instant invention is contemplated in one embodiment of the present invention. In some embodiments, lactoferrin may be used as the Tf portion of the fusion protein so that the fusion protein is produced and secreted in milk.

[0332] The successful production of transgenic, non-human animals has been described in a number of patents and publications, such as, for example U.S. Pat. No. 6,291,740 (issued Sep. 18, 2001); U.S. Pat. No. 6,281,408 (issued Aug. 28, 2001); and U.S. Pat. No. 6,271,436 (issued Aug. 7, 2001) the contents of which are hereby incorporated by reference in their entireties.

[0333] The ability to alter the genetic make-up of animals, such as domesticated mammals including cows, pigs, goats, horses, cattle, and sheep, allows a number of commercial applications. These applications include the production of animals which express large quantities of exogenous proteins in an easily harvested form (e.g., expression into the milk or blood), the production of animals with increased weight gain, feed efficiency, carcass composition, milk production or content, disease resistance and resistance to infection by specific microorganisms and the production of animals having enhanced growth rates or reproductive performance. Animals which contain exogenous DNA sequences in their genome are referred to as transgenic animals.

[0334] The most widely used method for the production of transgenic animals is the microinjection of DNA into the pronuclei of fertilized embryos (Wall et al., J. Cell. Biochem. 49:113 [1992]). Other methods for the production of transgenic animals include the infection of embryos with retroviruses or with retroviral vectors. Infection of both pre- and post-implantation mouse embryos with either wild-type or recombinant retroviruses has been reported (Janenich, Proc. Natl. Acad. Sci. USA 73:1260 [1976]; Janenich et al., Cell 24:519 [1981]; Stuhlmann et al., Proc. Natl. Acad. Sci. USA 81:7151 [1984]; Jahner et al., Proc. Natl. Acad. Sci. USA 82:6927 [1985]; Van der Putten et al., Proc. Natl. Acad. Sci. USA 82:6148-6152 [1985]; Stewart et al., EMBO J. 6:383-388 [1987]).

[0335] An alternative means for infecting embryos with retroviruses is the injection of virus or virus-producing cells into the blastocoele of mouse embryos (Jahner, D. et al., Nature 298:623 [1982]). The introduction of transgenes into the germline of mice has been reported using intrauterine retroviral infection of the midgestation mouse embryo (Jahner et al., supra [1982]). Infection of bovine and ovine embryos with retroviruses or retroviral vectors to create transgenic animals has been reported. These protocols involve the micro-injection of retroviral particles or growth arrested (i.e., mitomycin C-treated) cells which shed retroviral particles into the perivitelline space of fertilized eggs or early embryos (PCT International Application WO 90/08832 [1990]; and Haskell and Bowen, Mol. Reprod. Dev., 40:386 [1995]. PCT International Application WO 90/08832 describes the injection of wild-type feline leukemia virus B into the perivitelline space of sheep embryos at the 2 to 8 cell stage. Fetuses derived from injected embryos were shown to contain multiple sites of integration.

[0336] U.S. Pat. No. 6,291,740 (issued Sep. 18, 2001) describes the production of transgenic animals by the introduction of exogenous DNA into pre-maturation oocytes and mature, unfertilized oocytes (i.e., pre-fertilization oocytes) using retroviral vectors which transduce dividing cells (e.g., vectors derived from murine leukemia virus [MLV]). This patent also describes methods and compositions for cytomegalovirus promoter-driven, as well as mouse mammary tumor LTR expression of various recombinant proteins.

[0337] U.S. Pat. No. 6,281,408 (issued Aug. 28, 2001) describes methods for producing transgenic animals using embryonic stem cells. Briefly, the embryonic stem cells are used in a mixed cell co-culture with a morula to generate transgenic animals. Foreign genetic material is introduced into the embryonic stem cells prior to co-culturing by, for example, electroporation, microinjection or retroviral delivery. ES cells transfected in this manner are selected for integrations of the gene via a selection marker such as neomycin.

[0338] U.S. Pat. No. 6,271,436 (issued Aug. 7, 2001) describes the production of transgenic animals using methods including isolation of primordial germ cells, culturing these cells to produce primordial germ cell-derived cell lines, transforming both the primordial germ cells and the cultured cell lines, and using these transformed cells and cell lines to generate transgenic animals. The efficiency at which transgenic animals are generated is greatly increased, thereby allowing the use of homologous recombination in producing transgenic non-rodent animal species.

[0339] Gene Therapy

[0340] The use of modified transferrin fusion constructs for gene therapy wherein a modified transferrin protein or transferrin domain is joined to a therapeutic protein or peptide is contemplated in one embodiment of this invention. The modified transferrin fusion constructs with increased serum half-life or serum stability of the instant invention are ideally suited to gene therapy treatments.

[0341] The successful use of gene therapy to express a soluble fusion protein has been described. Briefly, gene therapy via injection of an adenovirus vector containing a gene encoding a soluble fusion protein consisting of cytotoxic lymphocyte antigen 4 (CTLA4) and the Fc portion of human immunoglubulin GI was recently shown in Ijima et al. (Jun. 10, 2001) Human Gene Therapy (United States) 12/9:1063-77. In this application of gene therapy, a murine model of type II collagen-induced arthritis was successfully treated via intraarticular injection of the vector.

[0342] Gene therapy is also described in a number of U.S. patents including U.S. Pat. No. 6,225,290 (issued May 1, 2001); U.S. Pat. No. 6,187,305 (issued Feb. 13, 2001); and U.S. Pat. No. 6,140,111 (issued Oct. 31, 2000).

[0343] U.S. Pat. No. 6,225,290 provides methods and constructs whereby intestinal epithelial cells of a mammalian subject are genetically altered to operatively incorporate a gene which expresses a protein which has a desired therapeutic effect. Intestinal cell transformation is accomplished by administration of a formulation composed primarily of naked DNA, and the DNA may be administered orally. Oral or other intragastrointestinal routes of administration provide a simple method of administration, while the use of naked nucleic acid avoids the complications associated with use of viral vectors to accomplish gene therapy. The expressed protein is secreted directly into the gastrointestinal tract and/or blood stream to obtain therapeutic blood levels of the protein thereby treating the patient in need of the protein. The transformed intestinal epithelial cells provide short or long term therapeutic cures for diseases associated with a deficiency in a particular protein or which are amenable to treatment by overexpression of a protein.

[0344] U.S. Pat. No. 6,187,305 provides methods of gene or DNA targeting in cells of vertebrate, particularly mammalian, origin. Briefly, DNA is introduced into primary or secondary cells of vertebrate origin through homologous recombination or targeting of the DNA, which is introduced into genomic DNA of the primary or secondary cells at a preselected site.

[0345] U.S. Pat. No. 6,140,111 (issued Oct. 31, 2000) describes retroviral gene therapy vectors. The disclosed retroviral vectors include an insertion site for genes of interest and are capable of expressing high levels of the protein derived from the genes of interest in a wide variety of transfected cell types. Also disclosed are retroviral vectors lacking a selectable marker, thus rendering them suitable for human gene therapy in the treatment of a variety of disease states without the co-expression of a marker product, such as an antibiotic. These retroviral vectors are especially suited for use in certain packaging cell lines. The ability of retroviral vectors to insert into the genome of mammalian cells have made them particularly promising candidates for use in the genetic therapy of genetic diseases in humans and animals. Genetic therapy typically involves (1) adding new genetic material to patient cells in vivo, or (2) removing patient cells from the body, adding new genetic material to the cells and reintroducing them into the body, i.e., in vitro gene therapy. Discussions of how to perform gene therapy in a variety of cells using retroviral vectors can be found, for example, in U.S. Pat. Nos. 4,868,116, issued Sep. 19, 1989, and 4,980,286, issued Dec. 25, 1990 (epithelial cells), WO89/07136 published Aug. 10, 1989 (hepatocyte cells), EP 378,576 published Jul. 25, 1990 (fibroblast cells), and WO89/05345 published Jun. 15, 1989 and WO/90/06997, published Jun. 28, 1990 (endothelial cells), the disclosures of which are incorporated herein by reference.

[0346] Without further description, it is believed that a person of ordinary skill in the art can, using the preceding description and the following illustrative examples, make and utilize the present invention and practice the claimed methods. For example, a skilled artisan would readily be able to determine the biological activity, both in vitro and in vivo, for the fusion protein constructs of the present invention as compared with the comparable activity of the therapeutic moiety in its unfused state. Similarly, a person skilled in the art could readily determine the serum half life and serum stability of constructs according to the present invention. The following working examples therefore, specifically point out the preferred embodiments of the present invention, and are not to be construed as limiting in any way the remainder of the disclosure.

EXAMPLES

Example 1

[0347] A fusion protein between modified Tf and an antifusogenic HIV-1 peptide (T-20) comprising the sequence is made by fusing one or more copies of the nucleotide sequence encoding the peptide to the nucleotide sequence of TF to produce a fusion protein with a peptide fused to the N- or C-terminus of Tf.

[0348] In one embodiment, the Tf portion of the fusion protein is engineered to not allow glycosylation when produced in yeast. As discussed above, human transferrin has two N-linked glycosylation sites at about N413 and about N611. The N-linked glycosylation site comprises the sequence N-X-S/T. In one embodiment, N (Asn) is changed to Q (Gln); other changes are contemplated such as Asn to Ala or Ser or any other amino acid.

[0349] Specifically, the N413 and N611 codons are converted to GAT and GAC by oligonucleotide directed mutagenesis using the dut- and ung-method. See Kunkel et al. (1985) Proc. Natl. Acad. Sci. 82:488-492). The mutagenic oligonucleotides 5′-GCAGAAAACTACGATAAGAGCGATAAT-3′ (SEQ ID NO: 9) and 5′-CTATTTGGAAGCGACGTAACTGACTGC-3′ (SEQ ID NO: 10) are synthesized and used to mutagenize the N413 and N611 codons according to the methods of Funk et al. (U.S. Pat. No. 5,986,067).

[0350] Receptor binding and/or iron or carbonate binding is then disrupted by mutating the following iron and/or carbonate ion binding residues:

4Iron bindingN domainC domainAsp 63(Asp 82 of SEQ ID NO: 2)Asp 392(Asp 411 of SEQ ID NO: 2)Tyr 95(Tyr 114 of SEQ ID NO: 2)Tyr 426(Tyr 445 of SEQ ID NO: 2)Tyr 188(Tyr 207 of SEQ ID NO: 2)Tyr 514 or 517(Tyr 533 or Tyr 536 SEQ ID NO:2)His 249(His 268 of SEQ ID NO: 2)His 585(His 604 of SEQ ID NO: 2)Carbonate ion bindingN domainC domainThr 120(Thr 139 of SEQ ID NO: 2)Thr 452(Thi 471 of SEQ ID NO: 2)Arg 124(Arg 143 of SEQ ID NO: 2)Arg 456(Arg 475 of SEQ ID NO: 2)Ala 126(Ala 145 of SEQ ID NO: 2)Ala 458(Ala 477 of SEQ ID NO: 2)Gly 127(Gly 146 of SEQ ID NO: 2)Gly 459(Gly 478 of SEQ ID NO: 2)

[0351] The production of mutants deficient in iron binding may be accomplished by numerous techniques. See U.S. Pat. No. 5,986,067. A D63S substitution may prepared using the method of Nelson, R. M. and Long, G. L. (1989) Analyt. Biochem. 180:147-151. Briefly, a HpalI/BamHI fragment from the 5′ end of the hTF/2N coding sequence is subcloned into pUC18 and then used as a template for a two step PCR-based mutagenesis procedure. The fragment is then released from the double stranded form of the sequencing vector by digestion with XbaI and BamHI and then ligated to a BamHLI/HindIII fragment from the original human Tf construct to produce a full length D63S-coding sequence, the fidelity of this splicing is confirmed by restriction digestion analysis.

[0352] For expression in Pichia the system from RCT/Invitrogen can be used. Three vectors are available for multicopy expression, pPIC9K, pPIC3.5K and pAO815. For this example the pPIC9K vector, which allows secretion into the growth medium, is used.

[0353] The modified transferrin sequence was cloned into the pPIC9K vector by altering the ends of the transferrin cDNA by overlapping PCR mutagenesis, this yielded the vector pREX0010. A number of restriction sites within the vector and coding sequence were removed or added to aid later cloning steps (FIG. 5).

[0354] The sequence for the HIV anti-fusogenic peptide DP-178 is also known as T-20. This peptide lends itself to fusion at the N- or C-termini of Transferrin, as the peptide may need freedom of movement to fulfill its function.

5DP-178 sequence:YTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWF(SEQ ID NO: 4)

[0355] When back translated in to DNA (using codons optimized for yeast) the following sequence was obtained (SEQ ID NOS: 13 and 14):

6tacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaatta y t s l i h s l i e e s q n q q e k n e q e lttggaattagataaatgggcaagtttgtggaattggtttl e l d k w a s l w n w f

[0356] To inset the above sequence the vector pREX0010 with the modified transferrin cDNA, was digested with the restriction enzymes Xba I/Kpn I for insertion at the 5′ end and Sal I/Hind III for insertion at the 3′ end.

[0357] For the 5′ insertion two overlapping oligos that form an Xba I overhang at the 5′ end and a Kpn I overhang at the 3′ end of the DP-178 sequence given above were synthesized. These oligos were then annealed together (see below) and ligated into the Xba I/Kpn I digested pREX0010 vector.

7KbaISEQ ID NOS: 15 and 16-----1ctagagaaaa ggtacactag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt tctttt ccatgtgatc gaattatgtg aggaattaac ttcttagcgt tttggtcgtt cttttcttac ttgttcttaa l e k r y t s l i h s l i e e s q n q q e k n e q e >>.................................T-20..................................>>>.........>> KpnI ---- 81attggaatta gataaatggg caagtttgtg gaattggttt gtactaaccttaat ctatttaccc gttcaaacac cttaaccaaal l ell llld k w a s l w n w f v >>>>>..................T-20..................>>

[0358] Insertion of the annealed oligos resulted in loss of the Kpn I site upon insertion.

[0359] This resulted in the vector pREX0011 (FIG. 6).

[0360] For insertion at the C-terminus a similar approach was taken by the addition of a SalI site at the 5′ end and a HindIII at the 3′ end (FIG. 7). Transformation, selection and expression are then performed as described in the Invitrogen Pichia Expression kit protocol booklet.

Example 2

[0361] INGAP fusions are prepared using a reverse translated human INGAP amino acid sequence. The protein sequence is as follows: sp|Q92778|PBCG_HUMAN Human INGAP

8MMLPMTLCRMSWMLLSCLMFLSWVEGEESQKKLPSSRITCPQGSVAYGSYCYSL(SEQ ID NO: 17)ILIPQTWSNAELSCQMHFSGHLAFLLSTGEITFVSSLVKNSLTAYQYIW[IGLHDPSHGTLPNG]GWKWSSSNVLTFYNWERNPSIAADRGYCAVLSQKSGFQKWRDFNCENELPYICKFKV

[0362] Reverse translated in to DNA (codons optimized for yeast) gave the following (SEQ ID NO: 18 and 19).

91atgatgttgc caatgacttt gtgtagaatg tcttggatgt tgttgtcttg tttgatgttt m m l p m t l c r m s w m l l s c l m f61ttgtcttggg ttgaaggtga agaatctcaa aaaaaattgc catcttctag aattacttgt l s w v e g e e s q k k l p s s r i t c121ccacaaggtt ctgttgctta tggttcttat tgttattctt tgattttgat tccacaaact p q g s v a y g s y c y s l i l i p q t181tggtctaatg ctgaattgtc ttgtcaaatg catttttctg gtcatttggc ttttttgttg w s n a e l s c q m h f s g h l a f l l241tctactggtg aaattacttt tgtttcttct ttggttaaaa attctttgac tgcttatcaa s t g e i t f v s s l v k n s l t a y q301tat [atttgga ttggtttgca tgatccatct catggtactt tgccaaatgg ttct]ggttgg y i w i g l h d p s h g t l p n g s g w361aaatggtctt cttctaatgt tttgactttt tataattggg aaagaaatcc atctattgct k w s s s n v l t f y n w e r n p s i a421gctgatagag gttattgtgc tgttttgtct caaaaatctg gttttcaaaa atggagagat a d r g y c a v l s q k s g f q k w r d481tttaattgtg aaaatgaatt gccatatatt tgtaaattta aagtt f n c e n e l p y i c k f k v

[0363] The most likely point for cleavage of the leader sequence is at the KK at the end of the underlined sequence above.

[0364] One methodology which may be used to generate constructs for the expression of INGAP fused to the N- or C-terminus of transferrin is to synthesize a series of overlapping oligos designed from the sequence given above (minus the underlined leader sequence). Annealing of these primers generates the INGAP cDNA. With different oligos designed for the 5′ and 3′ ends the annealed cDNA can be ligated into pREX0010 at the 5′ or 3′ end of Transferrin.

[0365] The bracketed sequence is the peptide used to induce INGAP activity. Hence the sequence could be paired down to some point between the whole and this minimal sequence.

[0366] N-terminal Fusion.

[0367] For the N-terminus these would have an overhang which forms an XbaI site at the 5′ end and an overhang compatible with a KpnI site at the 3′ end but which results in the destruction of the KpnI site.

10XbaISEQ ID NOS: 20 AND 21----1ctagagaaaa ggttgccatc ttccagaatt acttgtccac aaggttctgt tgcttatggt tctttt ccaacggtag aaggtcttaa tgaacaggtg ttccaagaca acgaatacca l e k r l p s s r i t c p q g s v a y g61tcttattgtt attctttgat tttgattcca caaacttggt ctaatgctga attgtcttgtagaataacaa taagaaacta aaactaaggt gtttgaacca gattacgact taacagaaca s y c y s l i l i p q t w s n a e l s c121caaatgcatt tttctggtca tttggctttt ttgttgtcta ctggtgaaat tacttttgttgtttacgtaa aaagaccagt aaaccgaaaa aacaacagat gaccacttta atgaaaacaa q m h f s g h l a f l l s t g e i t f v181tcttctttgg ttaaaaattc tttgactgct tatcaatata tttggattgg tttgcatgatagaagaaacc aatttttaag aaactgacga atagttatat aaacctaacc aaacgtacta s s l v k n s l t a y q y i w i g l h d241ccatctcatg gtactttgcc aaatggttct ggttggaaat ggtcttcttc taatgttttgggtagagtac catgaaacgg tttaccaaga ccaaccttta ccagaagaag attacaaaac p s h g t l p n g s g w k w s s s n v l301actttttaca attgggaaag aaatccatct attgctgctg atagaggtta ttgtgctgtttgaaaaatgt taaccctttc tttaggtaga taacgacgac tatctccaat aacacgacaa t g y n w e r n p s i a a d r g y c a v361ttgtctcaaa aatctggttt tcaaaaatgg agagatttta attgtgaaaa tgaattgccaaacagagttt ttagaccaaa agtttttacc tctctaaaat taacactttt acttaacggt l s q k s g f q k w r d f n c e n e l p KpnI ----421tatatttgta aatttaaagt tgtacatataaacat ttaaatttca a y i c k f k v v

[0368] Digestion of pREX0010 with XbaI and KpnI and ligation of the above sequence yields the vector pREX0013 (FIG. 8).

[0369] C-Terminal Fusion.

[0370] For the C-terminus the 5′ end would form a SalI site and at the 3′ end a stop codon plus a HindIII site.

11SalISEQ ID NOS 22 AND 23----1tcgacctttg ccatcttcca gaattacttg tccacaaggt tctgttgctt atggttctta ggaaac ggtagaaggt cttaatgaac aggtgttcca agacaacgaa taccaagaat r p l p s s r i t c p q g s v a y g s61ttgttattct ttgattttga ttccacaaac ttggtctaat gctgaattgt cttgtcaaataacaataaga aactaaaact aaggtgtttg aaccagatta cgacttaaca gaacagtttay c y s l i l i p q t w s n a e l s c q121gcatttttct ggtcatttgg cttttttgtt gtctactggt gaaattactt ttgtttcttccgtaaaaaga ccagtaaacc gaaaaaacaa cagatgacca ctttaatgaa aacaaagaagm h f s g h l a f l l s t g e i t f v s181tttggttaaa aattctttga ctgcttatca atatatttgg attggtttgc atgatccatcaaaccaattt ttaagaaact gacgaatagt tatataaacc taaccaaacg tactaggtags l v k n s l t a y q y i w i g l h d p241tcatggtact ttgccaaatg gttctggttg gaaatggtct tcttctaatg ttttgactttagtaccatga aacggtttac caagaccaac ctttaccaga agaagattac aaaactgaaas h g t l p n g s g w k w s s s n v l t301ttacaattgg gaaagaaatc catctattgc tgctgataga ggttattgtg ctgttttgtcaatgttaacc ctttctttag gtagataacg acgactatct ccaataacac gacaaaacagf y n w e r n p s i a a d r g y c a v l361tcaaaaatct ggttttcaaa aatggagaga ttttaattgt gaaaatgaat tgccatatatagtttttaga ccaaaagttt ttacctctct aaaattaaca cttttactta acggtatatas q k s g f q k w r d f n c e n e l p y HindIII —————421ttgtaaattt aaagtttaat aaacatttaaa tttcaaatta ttcgai c k f k v -

[0371] Digestion of pREX0010 with SalI and HindIII and ligation of the above sequence yields the vector pREX0014 (FIG. 9).

[0372] Transformation, selection and expression are then performed as described in the Invitrogen Pichia Expression kit protocol booklet.

Example 3

[0373] The peptide given below has been shown to mimic EPO activity by causing dimerisation of the EPO receptor. The peptide, which is cyclic, has no homology to EPO. For activity the peptide has to act in concert with another peptide, i.e. as a dimer, such that two copies of the receptor are brought in close enough proximity to form an active complex. As with many peptides the peptide dimer suffers from short half life and would benefit from the longevity that fusion to transferrin would give. In this example two peptides are engineered into the transferrin scaffold.

12SEQ ID NOS: 24 AND 251ggtggtactt actcttgtca ttttggtcca ttgacttggg tttgtaagcc acaaggtggt g g t y s c h f g p l t w v c k p q g g.

[0374] As detailed by Ali et al, a peptide can be successfully engineered into Transferrin between His289 and Gly290. The duplication inherent to the transferrin molecule, with the two domains mirroring each other, means that it is possible to engineer a peptide into the duplicate region of the C domain, between Glu625 and Thr626.

13N277D-KSKE--FQ LFSSPHGKDL LFKDSAHGFL KVPPRMDAKM YLGYEYVTAISEQ ID NOS: 26 AND 27C611NVTDCSGNFC LFRSE-TKDL LFRDDTVCLA KLHDRNTYEK YLGEEYVKAV.

[0375] For each insertion two overlapping mutagenic primers are synthesized (see below). Using pREX0010 as a template reactions were performed with each mutagenic primer and an external primer from the 5′ or 3′ of the Tf cDNA. The products from these two reactions were then mixed and a further reaction performed with the external primers to join the two products together. The His289-Gly290 insert PCR product was digested with XbaI and HpaI for ligation into XbaIlHpaI digested pREXOOO. The resulting vector was then digested with HpaI and SalI for ligation of HpallSalI digested the Glu625-Thr626 insert PCR product.

14His289-Gly290 insert (SEQ ID NO: 28). ←-------------2031agacaaatca[aaagaatttc aactattcag ctctcctcat ggtggtactt actcttgtca ttttggtccatctgtttagt tttcttaaag ttgataagtc gagaggagta ccaccatgaa[tgagaacagt aaaaccaggt >>.............EMOm..............>>....................................Tf....................................>>.................................N domain.................................>2101ttgacttggg tttgtaagcc]acaaggtggt gggaaggacc tgctgtttaa ggactctgcc cacgggttttaactgaaccc aaacattcgg tgttccacca cccttcctgg acgacaaatt cctgagacgg]gtgcccaaaa -----------→>............EMOm.............>>>....................................Tf....................................>>.................................N domain.................................>Glu625-Thr626 insert (SEQ ID NO: 29). ←-------------3081cctatttgga agcaacgtaa ctgactgctc[gggcaacttt tgtttgttcc ggtcggaagg tggtacttacggataaacct tcgttgcatt gactgacgag cccgttgaaa acaaacaagg ccagccttcc accatgaat [g >>...EPOm...>>.................................C domain.................................>>....................................Tf....................................> KpnI -------3151tcttgtcatt ttggtccatt gacttgggtt tgtaagccac]aaggtggtac caaggacctt ctgttcagagagaacagtaa aaccaggtaa ctgaacccaa acattcggtg ttccaccatg gttcctggaa gacaagtctc -----------→>......................EPOm.......................>>>.................................C domain.................................>>....................................Tf....................................>3221atgacacagt atgtttggcc aaacttcatg acagaaacac atatgaaaaa tacttaggag aagaatatgttactgtgtca]tacaaaccgg tttgaagtac tgtctttgtg tatacttttt atgaatcctc ttcttataca>.................................C domain.................................>>....................................Tf....................................>

[0376] These gave the plasmid pREX0015 (FIG. 10). Transformation, selection and expression are then performed as described in the Invitrogen Pichia Expression kit protocol booklet.

[0377] Alternative points for insertion of the EPO mimetic peptide(s), or any other peptide(s) are the two glycosylation sites on the C domain of Transferrin at N413 and N611. The advantage of this would be that insertion is achieved and glycosylation prevented, by disruption of the N-X-S/T sequence, in one and the same event.

Example 4

[0378] Fusion proteins between Tf and fusogenic inhibitor peptides against RSV are made by fusing the peptide sequences to the N- or C-terminal ends of Tf or by the insertion of the sequences into a loop of Tf, wherein the Tf is modified to not bind iron and/or is modified to prevent glycosylation. The RSV peptide may include: T786: VYPSDEYDASISQVNEEINQALAYIRKADELLENV (SEQ ID NO: 5) and/or T1584: AVSKVLHLEGEVNKIKSALLSTNKAVVSLSNGVSVLTSKVLDLKNYIDKQL (SEQ ID NO: 6).

[0379] The T786 peptide has a RK dipeptide which could act as a cleavage site for the yeast protease Kex2p. This would result in a truncated peptide. Accordingly, this peptide may be modified from RK to RE. Another version of the T786 peptide, T112 (VFPSDEFDASISQVNEKINQSLAFIRESDELLHNV, SEQ ID NO: 7), which is more potent than T786 has solubility problems in its unfused form. Accordingly, a version of T112 modified for the RK to RE is also made to produce a version of the peptide fused to Tf.

[0380] To produce the genetic constructs, the peptide sequences are backtranslated in DNA using codon bias for human, yeast or any other organism as appropriate.

Example 5

[0381] Various cytokines can be fused to the N-, C- or N- and C-termini of Tf. These fusions can also be constructed using different parts or domains of modified transferrin such as the N domain or C domain. The proteins can be fused directly or using a linker peptide of various lengths. It is also possible to fuse all or part of the active cytokine within the scaffold of transferrin.

[0382] The cDNA for the cytokine of interest, such as EPO, can be isolated by a variety of means such as RT-PCR from mRNA, from cDNA libraries, by synthetically constructing the cDNA from overlapping oligonucleotides, by PCR or by other means known to the art, all using standard methods. The nucleotide sequences for all of these proteins are known and available, for instance, in U.S. Pat. Nos. 4,703,008, 4,810,643 and 5,908,763 as well as in public databases such as GenBank. The cDNA can be tailored at the 5′ and 3′ ends to generate restriction sites, such that oligonucleotide linkers can be used, for cloning of the cDNA into a vector containing the cDNA for Transferrin. This can be at the N- or C-terminus, with or without the use of a spacer sequence, or by inserting the cDNA of the cytokine within the cDNA of Transferrin. The cytokine, e.g. EPO, and Tf cDNA are cloned into a vector from which the complete expression cassette is then excised and inserted into an expression vector to allow the expression of the fusion protein in yeast (or any other appropriate expression system). The fusion protein secreted from the yeast can then be collected and purified from the media and tested for its biological activity.

[0383] For expression in mammalian cell lines, a similar procedure is adopted except that the expression cassette used employs a mammalian promoter, leader sequence and terminator. This expression cassette is then excised and inserted into a plasmid suitable for the transfection of mammalian cell lines.

Example 6

[0384] Various interferons can be fused to the N-, C- or N- and C-termini of modified transferrin. These fusions can be constructed using different parts or domains of transferrin such as the N domain or C domain. The proteins can be fused directly or using a linker peptide of various lengths. It is also possible to fuse all or part of the interferon within the scaffold of transferrin.

[0385] A specific example of an interferon that can be fused to Tf is interferon-β. The cDNA for the interferon of interest such as IFN β can be isolated by a variety of means such as RT-PCR from mRNA or cDNA, from cDNA libraries, by synthetically constructing the cDNA from overlapping oligonucleotides, by PCR or by other means known to the art, all using standard methods. The nucleotide sequences for interferons, such as IFNα, IFNβ, and IFNγ are known and available, for instance, in U.S. Pat. Nos. 5,326,859 and 4,588,585, in EP 32 134, as well as in public databases such as GenBank. The cDNA can be tailored at the 5′ and 3′ ends to generate restriction sites, such that oligonucleotide linkers can be used to clone the cDNA into a vector containing the cDNA for modified transferrin. This can be at the N-, C- or N- and C-termini of the transferrin sequence, with or without the use of a spacer sequence. The IFN β (or other interferon) cDNA is cloned into a vector from which the complete expression cassette is then excised and inserted into an expression vector to allow the expression of the fusion protein in yeast. The fusion protein secreted from the yeast can then be collected and purified from the media and tested for its biological activity.

[0386] For expression in mammalian cell lines a similar procedure is adopted except that the expression cassette used employs a mammalian promoter, leader sequence and terminator. This expression cassette is then excised and inserted into a plasmid suitable for the transfection of mammalian cell lines. IFNs fused to transferrin have much longer half-life, thus, the therapeutic dosages of the fused proteins are much less than the IFNs. Therefore, the fused interferons are more efficacious with much less toxicity.

Example 7

[0387] Various single chain antibodies (SCA) were originally invented to simplify antibody selection and production. However, they prove to be of limited or no therapeutic values due to their small size and short in vivo half-life. Addition of transferrin to SCA significantly increases the in vivo half-life of SCA.

[0388] SCA can be fused to the N-, C- or N- and C-termini of modified transferrin. These fusions could also be carried out using different parts or domains of transferrin such as the N domain or C domain. The proteins could be fused directly or using a linker peptide of various length. It is also possible to fuse all or part of the active SCA within the scaffold of transferrin. In such instances the fusion protein is made by inserting the cDNA of the SCA within the cDNA of transferrin for production of the protein in cells. A specific example of a SCA that can be fused to Transferrin is anti-TNF (tumor necrosis factor). Anti-TNF has been used to treat various inflammatory and autoimmune diseases. TNF-SCA could be fused to the N- or C-terminus of modified transferrin in such manner that the coding N-terminus of TNF-SCA is directly attached to the C-terminal amino acid of Transferrin or the C-terminal amino acid of TNF-SCA is directly attached to the N-terminal amino acid of Transferrin. Alternatively, a peptide linker could be inserted to provide more separation between Transferrin and TNF-SCA and allow more spatial mobility to the two fused proteins. Several examples of TNF-SCA are shown in FIGS. 4A-4B.

[0389] Single chain antibodies are produced by several methods including but not limited to: selection from phage libraries, cloning of the variable region of a specific antibody by cloning the cDNA of the antibody and using the flanking constant regions as the primer to clone the variable region, or by synthesizing an oligonucleotide corresponding to the variable region of any specific antibody. The cDNA can be tailored at the 5′ and 3′ ends to generate restriction sites, such that oligonucleotide linkers can be used, for cloning of the cDNA into a vector containing the cDNA for transferrin. This can be at the N- or C-terminus or N- and C-termini with or without the use of a spacer sequence. The SCA molecule cDNA is cloned into a vector from which the complete expression cassette is then excised and inserted into an expression vector to allow the expression of the fusion protein in yeast. The fusion protein secreted from the yeast can then be collected and purified from the media and tested for its activity. For expression in mammalian cell lines a similar procedure is adopted except that the expression cassette used employs a mammalian promoter, leader sequence and terminator. This expression cassette is then excised and inserted into a plasmid suitable for the transfection of mammalian cell lines. The antibody produced in this manner can be purified from media and tested for its binding to its antigen using standard immunochemical methods.

Example 8

[0390] CDRs are the variable regions of antibodies that interact with antigens. These usually consist of relatively short stretches of peptides. Antibodies normally have three CDRs in their heavy chains and three in their light chains. One or more CDRs of an antibody which can interact with the antigen can be fused to modified transferrin to confer antigen binding activity to Transferrin molecule. The CDRs can be fused to the N-, C-, N- and C-termini or engineered into the interior scaffold of transferrin. Examples of the CDRs sequences from anti-TNF antibodies are shown in the TNF-SCA FIGS. 4A-4B. cDNAs corresponding to one or more CDRs can be fused with modified transferrin to confer TNF binding activity to transferrin.

Example 9

[0391] Transferrin fusion technology can also be used to improve the therapeutic properties of peptides that are discovered in various systems such as phage display libraries and peptide libraries. Many of these peptides have biological activities without any homology to natural proteins or peptides. These peptides, due to their short in vivo half-lives, are good candidates for fusion to modified transferrin. Because of their small size they can be fused in variety of regions of transferrin molecule. In addition to the N- and C-termini, they can be inserted in various regions within Transferrin including but not limited to the cystine loops. In this manner the three-dimensional structure of the peptide within transferrin stays relatively rigid. More than one copy of each peptide and more than one peptide can be fused to modified transferrin. Moreover, the peptide sequence may be used to replace portion of transferrin to confer therapeutic activity to transferrin. Since most of these peptides are short, their cDNA can be synthesized with appropriate restriction sites for insertion into the modified transferrin cDNA. The cDNA could then be inserted in a vector containing the transferrin cDNA in such a manner that the peptide is expressed as part of transferrin or fused to transferrin molecule. Alternatively, PCR primers could be synthesized that contain the peptide of interest and appropriate section of Transferrin. Using these primers amplification of transferrin eDNA results in the fusion of the peptide to the chosen site on Transferrin. Examples of such peptides are the EPO mimetic peptides: GGTYSCHFGPLTWVCKPQGG (SEQ ID NO: 11); DREGCRRGWVGQCKAWFN (SEQ ID NO: 12); and QRVEILEGRTECVLSNLRGRTRY (SEQ ID NO: 30), which have no homology with the natural EPO but have similar biological activities in that they activate the EPO receptor acting as agonists. These peptides also need to have specific conformation for their optimal activity. EPO mimetic peptides can be inserted (or it can replace) in one or more cystine loops of Transferrin. In this manner Transferrin can acquire EPO activity. Other peptides that can be fused to Transferrin are peptides with binding activity similar to antibodies. These peptides can bind to proteins with relatively high affinity and provide the same biological function as antibodies except their in vivo half-life is very short. Fusion of these peptides to Transferrin could confer much longer half-life for these peptides without destroying their binding activities. These peptides could be fused to N- or C-terminus or both or within the Transferrin molecule. The peptides can also replace part of transferrin. In addition more than one copy of a peptide or several different peptides could be attached to a single transferrin molecule. An example of such molecule is a peptide that can bind TNF. Attachment of this peptide to Transferrin gives Transferrin the ability to bind TNF and act similar to anti-TNF antibodies. In this manner antibody like molecules with much easier and economical manufacturing protocol could be made.

Example 10

[0392] Targeted Tf fusion proteins have a combination of two or more proteins or peptides fused to modified transferrin to serve as a bifunctional molecule. In this case modified transferrin is fused to one protein or peptide to have a new biological activity and to another protein or peptide to targeting. An example of such protein is a transferrin that contains an inhibitory protein such as endostatin and a targeting peptide such as SCA or binding peptide which can recognize tumours. In this manner the inhibitory molecule is targeted to the tumour where it is needed. The cDNA for the protein of interest can be isolated from cDNA library or can be made synthetically using several overlapping oligonucleotide primers using standard molecular biology methods. The appropriate nucleotides can be engineered in the eDNA to form convenient restriction sites and also allow the attachment of the protein cDNA to transferrin cDNA similar to the method described for other fusions. Also a targeting protein or peptide cDNA such as single chain antibody or peptides, such as nuclear localization signals, that can direct proteins inside the cells can be fused to the other end or within transferrin. The protein of interest and the targeting peptide is cloned into a vector, which allows the fusion with transferrin cDNA. In this manner both proteins/peptides are fused to modified transferrin. The fused cDNA is then excised and is inserted into an expression vector to allow the expression of the fusion protein in yeast.

[0393] All the above procedures can be performed using standard methods in molecular biology. The fusion protein secreted from yeast can be collected and purified from the media and tested for its biological activity and its targeting activity using appropriate biochemical and biological tests. These proteins could also be made in other systems such as mammalian tissue culture using appropriate vector and transfection protocol.

Example 11

[0394] The cDNA for the enzyme of interest can be isolated by a variety of means such as RT-PCR from mRNA, from cDNA libraries, by synthetically constructing the cDNA from overlapping oligonucleotides, by PCR or by other means known to the art, all using standard methods. The cDNA can be tailored at the 5′ and 3′ ends to generate restriction sites, such that oligonucleotide linkers can be used, for cloning of the cDNA into a vector containing the cDNA for modified transferrin. This can be at the N or C-terminus with or without the use of a spacer sequence. The enzyme cDNA is cloned into a vector such from which the complete expression cassette is then excised and inserted an expression vector to allow the expression of the fusion protein in yeast. The fusion protein secreted from the yeast can then be collected and purified from the media and tested for its biological activity. For expression in mammalian cell lines a similar procedure is adopted except that the expression cassette used employs a mammalian promoter, leader sequence and terminator. This expression cassette is then excised and inserted into a plasmid suitable for the transfection of mammalian cell lines.

Example 12

[0395] Using phage display, peptides are isolated specific for a specific cell marker on the surface of, for example, a tumor cell. The peptide is then fused to the N-, C- or N- and C-termini of modified transferrin to target the fusion to that specific cell type. The transferrin fusion protein is then loaded with a metal ion which resembles iron in its transferrin binding properties, but which is cytotoxic, for example gallium or radioactive ions. By this mechanism the gallium or the radioactive ion is targeted to the cell type.

[0396] Although the present invention has been described in detail with reference to exampes above, it is understood that various modifications can be made without departing from the spirit of the invention. Accordingly, the invention is limited only by the following claims. All cited patents, patent applications and publications referred to in this application are herein incorporated by reference in their entirety.

	Number	Date	Country
	60315745	Aug 2001	US
	60334059	Nov 2001	US

Modified transferrin fusion proteins

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