Claims
- 1. A polynucleotide molecule encoding an immunoglobulin fragment, said immunoglobulin fragment comprising a first DNA sequence encoding a first pectate lyase secretion signal sequence operably linked to a DNA sequence encoding an immunoglobulin Fd molecule or a fragment thereof comprising the variable region and a second DNA sequence encoding a second pectate lyase secretion signal sequence operably linked to a DNA sequence encoding an immunoglobulin light chain or a fragment thereof comprising the variable region, wherein said first and second DNA sequences are operably linked to a single prokaryotic promoter so as to form a dicistronic transcription unit, wherein said immunoglobulin fragment is capable of binding to an antigen and said immunoglobulin fragment is produced and secreted by an E. coli host cell when said polynucleotide molecule is expressed in said host cell.
- 2. A vector comprising the polynucleotide molecule of claim 1.
- 3. An E. coli host cell transformed with the vector of claim 2.
- 4. The polynucleotide molecule of claim 1, wherein said immunoglobulin Fd molecule or said immunoglobulin light chain are selected such that the resultant immunoglobulin fragment is chimeric.
Parent Case Info
This application is a continuation of U.S. application Ser. No. 07/987,555, filed Dec. 8, 1992 (abandoned) which is a continuation of U.S. application Ser. No. 07/501,092, filed Mar. 29, 1990 (abandoned), which is a continuation-in-part of U.S. application Ser. No. 07/077,528, filed Jul. 24, 1987 (abandoned), which is a continuation-in-part of PCT/US86/02269, filed Oct. 27, 1986 (abandoned), which is a continuation-in-part of U.S. application Ser. No. 06/793,980, filed Nov. 1, 1985 (abandoned); U.S. application Ser. No. 07/501,092, filed Mar. 29, 1990 is also a continuation-in-part of U.S. application Ser. No. 07/142,039, filed Jan. 11, 1988 (abandoned).
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Date |
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Jul 1987 |
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Continuations (2)
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Number |
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987555 |
Dec 1992 |
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Continuation in Parts (2)
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142039 |
Jan 1988 |
|
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793980 |
Nov 1985 |
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