The present invention relates to the field of dispensing equipment. More particularly, the present invention is directed to multi-vial dispensing of radiopharmaceuticals.
At the end of the radiosynthesis in clinical trial PET manufacturing, the bulk formulation is normally dispensed into three separate vials, the product collected in the first vial to be used for QC, the product collected in the second vial to be used for measurement of sterility and the product collected in the third vial to be used as the patient dose. The present setup at clinical sites is to use a customized dispenser that uses valves and a syringe driver to dispense the required volume according to a pre-programmed sequence. The metering of the product fluid needs to be precise so that requisite amounts of product are provided for each purpose. Additionally, particularly in radiopharmaceutical applications, there is a need to minimize any residual product within the fluid conduits to each dispense vial both to minimize operator exposure to residual activity and to reduce waste of the product fluid.
There is therefore a need for a system and method for dispensing into multiple product vials which provides precise metering of a product fluid and minimizes the occurrence of residual product within fluid delivery lines.
A new dispensing process is proposed that allows for simple and rapid dispensing of a radiopharmaceutical without the requirement for special dispensing apparatus. Retro installation at PET manufacturing sites is simplified and very little space is required. Furthermore the process minimizes the amount of the radiotracer lost in the transfer lines. The dispensing principle involves several vials each connected in series via a transfer line with the depth of the syringe needle in the vial defining the dispense volume.
The dispenser delivers the required volume for QC, microbiological and one or more patient samples to a reasonable accuracy and can be used for multiple vials. All the transfer lines between the vials are sucked or blown out as part of the dispensing process, thus minimizing losses within the transfer lines. This is a considerable simplification of the present dispensing apparatus used in clinical sites.
It is contemplated that the present invention may be applicable for other aseptic dispensing situations as well.
The method and essential components of the present invention are depicted in
Referring to
Once all liquid has been transferred to vial 10, the transfer to the second and third vial can be achieved either through continued positive pressure through fill conduit 42 and fill needle 40 or by applying negative pressure from vent needle 70 so as to draw the fluid from vial 10 into and/or through vials 20 and 30.
Where positive pressure is used, there must be sufficient nitrogen used to effect the transfer of liquid between all 3 vials and this will require an extra blow-out of the vials after the liquid has been dispensed. This is readily achievable using a FASTlab® synthesizer, sold by GE Healthcare of Liege, Belgium (although other automated synthesis or dispense systems may also be used), by opening the nitrogen buffer valve with a positive pressure of nitrogen. Because the whole process relies on gas pressure, fluid lines 50 and 60 are emptied thoroughly, thus maximizing recovery of the radiotracer. Such zero dead volume is not easily achievable with the current generic dispenser system presently used. It is contemplated by the present invention that vent needle 70 may also support, or be connected to, a valve which may be open and closed so as to allow the motive gas to pass therethrough and to prevent the backflow of gas back into vial cavity 34 through vent needle 70. Alternatively, vent needle 70 may be connected to a flexible conduit which may be left undeflected during dispense operations but which may be clamped or clipped closed so as to prevent gas backflow into vial cavity 34. As used herein, a clip or a clamp operates to pinch a deflectable conduit so as to collapse its internal lumen and thus provide a fluid-tight seal.
Where negative pressure is applied to vial 30, air is sucked out of this vial initially and this then sucks air out of the other two vials in turn taking solution with it. The final point where solution is no longer transferred is the same as for positive pressure and again all the fluid lines 50 and 60 are sucked dry. For example, present invention contemplates that a flexible conduit (not shown) may be connected to vent needle 70 at a location outside of vial 30, and desirably on the far side of the filter from needle 70, so that negative pressure may be applied to vent needle 70 and thus cause the drawing of the product fluid through the vials as described herein.
The whole process should be complete within 30 seconds, a significant time saving over present dispensing apparatus.
Though 3 vials is normally required for clinical sites, with sufficient nitrogen gas pressure or vacuum, the present invention is scalable to dispense into more than three vials serially connected using fluid lines of the present invention.
Prior to separating vials 10, 20 and 30 from their respective fill and withdrawal needles, the present invention further contemplates applying clips, or clamps, to conduits 52 and 62 so as to pinch each closed. With conduits 52 and 62 pinched closed, the threat of contamination through the exposure of one needle of the fluid line while the other is still inserted into its respective vial cavity will be obviated. As will be seen hereinbelow, the clips that are applied may be standard alligator-type clips or may be clips built into either a cassette of the present invention or a needle holder of the present invention which is attached to each vial. Additionally, it is contemplated by the present invention to ensure that vent needle 70 may be blocked so as to prevent outside air from entering into vial cavity 34 therethrough, desirably by blocking any conduit connected thereto. The blocking may be accomplished by applying a clip, or clamp, to a flexible conduit connected to vent needle 70 used for applying a vacuum or conducting a motive gas therethrough. Additionally, a valve may be incorporated into that conduit so as to block backflow of outside gas into vial cavity 34.
The present invention further contemplates that needle holders may be employed to hold the fill and withdrawal needles at each vial. Such needle holders attach to the vials and provide for passage of the needles therethrough so that each needle will be inserted through the vial septum and into the vial cavity. Such needle holders are desirably formed from a suitable polymeric material such as PEEK. These needle holders are also designed for use with specified needles and specified vials so as afford controlled needle insertion depth. Most likely only two types would be required, giving either 3 ml or 10 ml fill in P6 type vials.
Referring now to
Referring now to
Referring now to
Clip 190 of needle holder 180 sits atop body 182. A cradle 192 defines conduit receiving recesses 193a and 193b open to receive conduits 52 and 62 therein, respectively. A locking clip 194 is slideable across the top of cradle 192 from a first position allowing fluid to flow through each conduit to a second position in which the clip is caused to compress conduits 52 and 62 against cradle 192 so as to pinch each closed. Clip 194 includes a first pair of detents 195a and 195b extending through locking head 196. Second pair of detents 197a and 197b extend from clip 194 and are insertable through head 196 until reaching the other side where each springs back to hold clip 194 in a position which pinches conduits 52 and 62. The present invention further contemplates that clip 190 additionally includes a depending hook. The hook is connected to locking clip 194 and positively engages either the cap or vial on which clip 190 is positioned while locking clip is in the first position and is moved clear of the cap or vial while locking clip is the second position. The hook will thus ensure that the vial cannot be removed from the conduits until the conduits for each needle are sealed by locking clip 194.
The present invention further contemplates employing a needle-less connection among the vials. Each of the vials may include a cap which provides inlet and outlet ports in fluid communication with the vial cavity. The inlet and outlet ports may further include valves which may be connected to conduit lines. The outlet port is further connected in fluid communication to an elongate hollow withdrawal conduit extending into the vial cavity a known distance. Each of the vials may then be placed in fluid communication with each other between a fill conduit and a vent conduit. Dispensing into the vials would be by the method of the present invention of driving fluid from one vial to the next based on the insertion depth of the withdrawal conduit extending to the outlet port of the cap.
Referring now to
Desirably, the transfer between the vials is effected by negative pressure from either an external vacuum source or the vacuum incorporated into a FASTlab synthesizer 208. The initial transfer and mixing occurs in to the pre-filled bulk vial 202 with vent 206 present to stop any build-up of gas pressure that may cause the fluid to transfer to the QC vial 210. Once this is filled, a vacuum is applied to the vent needle 270 at vial 240 which sucks air through from the vent through the 0.22 μm aseptic filter 209 in the direction indicated by arrow V. Alternatively, synthesizer 208 may provide the negative pressure through a conduit connected between it and asceptic filter 209a which also is in fluid communication with the cavity of vial 240. Once air enters the aseptic filter 204, no further flow of solution can occur and a negative pressure starts building up in the dispensed vials effecting transfer of solution. The transfer line between the aseptic filter 204 and vial 210 will remain full of solution since no gas can easily pass through the filter. However, each subsequent transfer line will be emptied as the pressures equalizes between the vials. The conduit lines spanning between the vials are desirably clamped so as to seal the lumens of each conduit prior to removing the needles from their respective vials, ensuring sterility of the vial contents.
It is important that the pressure differential across the aseptic 0.22 μm 204 filter is not great enough to cause rupturing of the membrane.
Once all the samples are dispensed it is necessary to test the integrity of the aseptic filter 204 and this can be done through a proprietary bubble point apparatus 211 connected above it or possibly by applying increasing pressure to the filter and observing when air is forced through the filter. The QC vial 210 is desirably disconnected for this latter option to prevent any chance of contamination.
The cassette of the present invention may be assembled for dispensing into multiple vials using a fluid line spanning between successive vials. Each vial includes a vial body defining a vial cavity and is sealed by an elastomeric septum. Each fluid line includes an elongate conduit supporting an elongate needle at each end thereof, a withdrawal needle at the first end and a fill needle at the opposed second end. The cassette may be assembled by the steps of first inserting a first fill needle into a first vial, then inserting the free end of a first withdrawal needle of a first fluid line into the first vial, whereby the free end of the first withdrawal needle of the first fluid line extends at least as low into said vial cavity of the first vial as the first fill needle. A second fill needle connected at the opposite end of the first fluid line from the first withdrawal needle is inserted into a second vial such that its free end extends through the elastomeric septum of the vial and into the vial cavity of the second vial. A second withdrawal needle supported at the first end of a second fluid line is inserted into the second vial such that the free end thereof extends through the elastomeric septum of the second vial and into the vial cavity. The second withdrawal needle of said second fluid line extends at least as low into the vial cavity of the second vial as the second fill needle of the first fluid line. A third fill needle affixed to the opposite end of the second fluid line from the second withdrawal needle is inserted into a third vial such that the free end thereof extends through the septum of the third vial and into the vial cavity of the third vial. A vent needle is inserted into the third vial such that the free end thereof extends through the elastomeric septum of the third vial and into the vial cavity thereof. With the cassette assembled, the vent needle will be in fluid communication with the first vial cavity. The cassette may also be provided in kit form, providing the vials, needles, conduit lines, and vent adapted for assembly. Desirably, the kit of the present invention provides the fill and withdrawal needles sealingly connected to their respective conduits.
For dispensing operations, the first fill needle is placed in fluid communication with a source of product fluid to be dispensed, such that the product fluid may be directed through the fill needle into the first vial. The product fluid from the fluid source is then directed through the first fill needle into said first vial, at least some of the fluid being further directed through said first fluid line to said second vial and through said second fluid line to said third vial. The motive force for directing the fluid into each vial may be a positive pressure acting on the fluid source or a negative pressure acting through the vent needle (or otherwise through the third vial).
The present invention contemplates that the insertion of the first fill needle and the vent needle may occur last, such that the cassette of the present invention is otherwise assembled. This cassette may be assembled just prior to dispense operations, or it may be assembled long before dispense operations, the typical limitation being the ability of the septums to re-seal once the fill and withdrawal or vent needles are removed.
The present invention contemplates that the free ends of the needles may be positioned either partway into the septums such that they do not penetrate into the respective vial cavity but do not expose the needle lumens to the atmosphere or that the free ends of the needles may themselves be capped by an elastomeric plug which may be positioned in contact with the respective vial septum so that the needle may push through both the plug and the septum (ie, without removing the plug and exposing the needle lumen) when penetrating into the vial cavity. In this manner, the cassette of the present invention may be assembled in a clean or sterile environment and the sterility of the fluid-contacting surfaces of the needles, conduits and vials may maintain sterility regardless of the outside environmental conditions.
Additionally, the present invention provides a method of dispensing into multiple vials using a number of fluid lines, where each vial includes a vial body defining a vial cavity and sealed by cap which includes an inlet port and an outlet port through which fluid may be provided into and out of said vial cavity, respectively. The inlet port is in fluid communication with a fill conduit extending into its respective vial cavity and the outlet port is in fluid communication with a withdrawal conduit extending at least as low into the vial cavity of the first vial as the fill conduit. Each fluid line includes an elongate conduit supporting a first connector at one end for connection to the out port of a vial and a second connector at the opposite end for connection to the inlet port of a vial. The method includes the steps of connecting a fill conduit to an inlet port of a first vial, connecting the first connector of a first fluid line to an outlet port of the first vial, connecting the second connector of the first fluid line to an inlet port of a second vial, connecting the first connector of a second fluid line to the outlet port of the second vial, connecting the second connector of the second fluid line to an inlet port of a third vial, connecting a vent conduit to the outlet port of the third vial, directing a fluid from a fluid source through the fill conduit into the first vial, at least some of the fluid being further directed through the first fluid line to the second vial and through the second fluid line to the third vial. The directing step may also include the step of applying a motive force to the fluid source so as to direct the fluid into the fill conduit. The motive force may be either a positive pressure applied to the fluid source to direct fluid to the fill conduit or a negative pressure applied through the third vial so as to draw the fluid from the fluid source to each vial.
The present invention further provides a method of forming a multi-vial dispense cassette which may be used to dispense a product fluid to a number of serially-connected vials, wherein each vial includes a vial body defining a vial cavity and is closed by an elastomeric septum. The cassette includes a first fluid line for extending in fluid communication between the vial cavities of a first and second vial, the first fluid line including an elongate hollow conduit supporting an elongate hollow needle at each end thereof. The cassette also includes a second fluid line for extending in fluid communication between the vial cavities of the second vial and a third vial, the second fluid line including an elongate hollow conduit supporting an elongate hollow needle at each end thereof. The cassette provides for a vent needle to be insertable into the vial cavity of third vial, the vent needle being connectable to, or including, a filter so as to protect the contents of the vials from contamination. Additionally, the dispense cassette allows a fill needle to be insertable into the vial cavity of the first vial, the fill needle being connected to a source of product fluid. The present invention also contemplates that the each of the needles provided are sealed by a pierceable cap, possible formed of an elastomeric material. Each needle may be inserted through its pierceable cap and into the septum of its respective vial so as to maintain the sterility of the fluid path to be seen by a product fluid dispensed into the vials.
The present invention further contemplates that the cassette and the fill needle and vent needles may be provided in kit form to be assembled by a user prior to dispensing. The cassette may come in either an assembled or unassembled configuration, such that each of the parts are adaptable to be connected together so as to form the dispense cassette. The kit may further include the vent needle and fill needle to be used for a dispensing operation, although the fill needle and/or the vent needle may be provided by a dispensing system to which the cassette attaches.
While the particular embodiment of the present invention has been shown and described, it will be obvious to those skilled in the art that changes and modifications may be made without departing from the teachings of the invention. The matter set forth in the foregoing description and accompanying drawings is offered by way of illustration only and not as a limitation. The actual scope of the invention is intended to be defined in the following claims when viewed in their proper perspective based on the prior art.
Filing Document | Filing Date | Country | Kind | 371c Date |
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PCT/US11/68115 | 12/30/2011 | WO | 00 | 6/26/2013 |
Number | Date | Country | |
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61428755 | Dec 2010 | US |