Mutant human insulin receptor DNA

This application is a 371 of PCT/JP95/00906, filed May 12, 1995.
1. Technical Field
This invention relates to abnormalities in the insulin receptor structure gene in non-insulin-dependent diabetes mellitus (hereinafter referred to simply as NIDDM). More particularly, it relates to mutant human insulin receptor DNAs having mutations at specific sites and fragments thereof.
2. Background Art
NIDDM, which is a genetic disease with one of the highest incidence in mankind today, is induced by several gene abnormalities combined with environmental factors such as obesity, stress and aging. In Japan, the number of patients with NIDDM is estimated to be about 5,000,000. NIDDM has been recently designated as one of the four most serious diseases following cancer, cerebral stroke and heart infarction. Thus there has been an urgent need to establish an effective countermeasure for NIDDM. In general, the symptoms of NIDDM can be frequently ameliorated by diet therapy and kinesitherapy. Therefore, if possible, it is best to diagnose NIDDM at an early stage. Presently, in order to diagnose NIDDM at an early stage, troublesome examinations, such as OGTT, must be conducted. OGTT comprises orally administering 75 g of glucose to a patient in a fasting state, collecting the blood at intervals of 30 minutes and measuring the blood sugar level at two hour intervals. Therefore, a more convenient and reliable diagnostic method is needed for early detection and prevention of NIDDM.
Although no gene has been found to be responsible for the onset of NIDDM, it is assumed that genes of insulin function mechanism-relating factors or genes of insulin secretion-relating factors may be candidate genes for NIDDM. It is known that the factors relating to insulin function involve insulin receptor, insulin receptor substrate (IRS-1), glucose transporter type 4, etc., while the factors relating to insulin secretion involve glucose transporter type 2, glucokinase, chondriogene, etc. Although attempts were made to detect abnormalities in the latter two genes in association with NIDDM, the abnormality ratio was only around 1% in each case (Interim Report in 1993, Onset Mechanism Group, Research and Study Project on Diabetes, Ministry of Health and Welfare).
Insulin acts upon the target cell by binding to the insulin receptor located on the cell membranae. Insulin resistance is often observed in the early stages of NIDDM (Taylor, S. I. Diabetes 41:1473-1490, 1992). Based on these facts, it has been speculated that the insulin receptor might be the gene responsible for the onset of NIDDM. Abnormalities in the insulin receptor would result in a high insulin resistance and thus induce severe diabetes accompanied by hyperinsulinemia. However, such a phenomenon is scarcely observed in NIDDM cases. Thus, abnormalities in the insulin receptor have not been related to NIDDM.
In recent years, a number of insulin receptor abnormalities have been discovered by others including the present inventors. It has been found that the examination data and symptoms of patients vary widely depending on the mutation type (M. Taira et al., Science 245:63-66, 1989; F. Shimada et al., Lancet 335:1179-1181, 1990). Thus, it is quite possible that insulin receptor gene abnormalities may partially participate in the onset of NIDDM. However, no attempt has been made so far to systematically detect the insulin receptor gene abnormalities on a large scale in association with NIDDM. In addition, the particular locations of the gene abnormalities are still unknown.
Under these circumstances, the present inventors have prepared chromosomal DNAs from the blood of typical NIDDM Japanese patients and studied the base sequences of insulin receptor DNAs in order to reveal the relationship between human insulin receptor gene abnormalities and NIDDM. As a result, they have found out that a quantitative abnormality is observed at a significant frequency in the patients with NIDDM, thus completing the present invention.

BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is an electrophorogram which shows part of the base sequence of the exon 13 of the insulin receptor .beta.-subunit of five patients with NIDDM.
FIG. 2 is an electrophorogram which shows part of the base sequence of the exon 22 of the insulin receptor .beta.-subunit of eight patients with NIDDM.
FIG. 3 shows insulin binding (A) and receptor tyrosine kinase activity (B,C) of wild type (IR.sup.WT) and the mutant (IR.sup.A831) receptors. (A) Confluent CHO cells expressing IR.sup.WT (clone No. 12: .smallcircle. - - - .smallcircle., clone No. 21: .smallcircle. - - - .smallcircle.) or IR.sup.A831 (clone No. 10: .DELTA. - - - .DELTA., clone No. 17: .DELTA. - - - .DELTA.) were incubated with .sup.125 I-insulin of various concentrations at 4.degree. C. for 12 hours. The radioactivity of the bound .sup.125 I-insulin was measured and plotted in accordance with the method of Scatchard. (B) Insulin-dependent receptor autophosphorylating activity was shown as the amount of .sup.32 P incorporated into the insulin receptor .beta.-subunit with the use of the same cell lines as those employed in (A). (C) An electrophorogram of the immunoblotting of insulin-dependent tyrosine phosphorylations of IRS-1 and IR .beta.-subunits measured with an anti-phospho-tyrosine antibody with the use of the same cell lines as those employed in (A).
FIG. 4 shows insulin-induced complex formation of the normal insulin receptor and two artificially mutated insulin receptors (IR.sup.A831 and IR.sup.C1334) and the p85 subunit of PI 3-kinase. The p85 subunit of PI 3-kinase was transiently co-expressed with the wild type IR (IR.sup.WT) or the mutant IRs (IR.sup.A831 and IR.sup.C1334) in COS cells, respectively. In the presence of 10.sup.-7 M of insulin, the cell lysates were immunoprecipitated by an anti-.beta.-subunit of IR (.alpha.-IR.beta.) or anti-p85 (.alpha.p85) antibodies. (A) and (B) are electrophorograms obtained by immunoblotting the immunoprecipitates with (A) an anti-phosphotyrosine antibody (.alpha.PY) or (B) an anti-.alpha.-subunit of IR antibody (.alpha.IR.alpha.).
FIG. 5 shows analysis on the relationship between the mutation and the onset of NIDDM in a pedigree with the mutant IR.sup.C1334. (A) Familial analysis of TAC (Tyr.sup.1334).fwdarw.TGC (Cys.sup.1334) replacement. Squares and circles stand respectively for male and female. Closed symbols stand for patients with NIDDM, the arrow shows the proband and slashed symbols stand for deceased. (B) Allele-specific hybridization. PCR products originating from the insulin receptor exon 22 of the genomic DNAs of three patients and a normal subject were separated in an agarose gel and transferred onto a nitrocellulose filter. After hybridizing with a .sup.32 P-labeled oligonucleotide probe specific to the mutant allele, the filter was autoradio-graphed. The IR.sup.C1334 mutation (a 346 bp fragment) was identified in one allele of the proband (Y. Y.) but not in a brother (T. S.) and a sister (S. K.).
FIG. 6 shows an analysis of the relationship between the mutation and the onset of NIDDM in a pedigree with the mutant IR.sup.A831. (A) Familial analysis of ACG (Thr.sup.831).fwdarw.GCG (Ala.sup.831) replacement. The IR.sup.A831 mutation was identified in one allele of the proband (Yh. T.), two brothers (I. T. and Yo. T.) and a sister (T. O.). The arrow shows the proband, squares and circles stand respectively for male and female. Closed symbols stand for patients with NIDDM, shaded symbols stand for IGT (impaired glucose tolerance) and slashed symbols stand for deceased. (B) An electrophorogram showing the detection of IR.sup.A831 by the restriction enzyme digestion. The IR.sup.A831 mutation in the PCR fragment of the exon 13 cleaved a cleavage site specific for the restriction enzyme CfoI. The CfoI-digestion product of the mutated PCR fragment (332 bp) resulted in the appearance of two bands (102 bp and 220 bp), while this cleavage was prevented in the wild type.
FIG. 7 shows DNA (SEQ ID NO:1) (No. 1) encoding human insulin receptor.
FIG. 8 shows DNA (No. 2) encoding human insulin receptor.
FIG. 9 shows DNA (No. 3) encoding human insulin receptor.
FIG. 10 shows DNA (No. 4) encoding human insulin receptor, wherein the mutation part (the amino acid at the 831-position) is boxed.
FIG. 11 shows DNA (No. 5) encoding human insulin receptor, wherein the mutation part (the amino acid at the 1334-position) is boxed.

DETAILED DESCRIPTION OF THE INVENTION
The present invention provides a mutant human insulin receptor DNA, wherein the base sequence encoding Thr.sup.831 in human insulin receptor DNA has been replaced by a base sequence encoding Ala and/or the base sequence encoding Tyr.sup.1334 therein has been replaced by a base sequence encoding Cys, or a fragment of this mutant human insulin receptor DNA containing the mutation part(s).
The insulin receptor is a biomembrane receptor which specifically binds to insulin and thus transmits the information to the inside of cells. It consists of two .alpha. chains (735 residues, molecular weight: 84, 214) and two .beta. chains (620 residues, molecular weight: 69, 700). The insulin receptor gene consists of 22 exons among which 11 exons encode the .alpha. subunits while other 11 exons encode the .beta.-subunits (S. Seino et al., Proc. Natl. Acad. Sci. USA 86:114-118, 1989). The base sequence of human insulin receptor and the amino acid sequence corresponding thereto are shown in SEQ ID NO:1 in Sequence Listing. The mutant human insulin receptor DNA of the present invention is one wherein the base sequence (ACG) encoding Thr.sup.831 in the exon 13 of the .beta.-subunit of human insulin receptor DNA has been replaced by a base sequence (GCG) encoding Ala and/or the base sequence (TAC) encoding Tyr.sup.1334 in the exon 22 of the .beta.-subunit has been replaced by a base sequence (TGC) encoding Cys, and a fragment of said mutant human insulin receptor DNA containing the mutation. The mutation parts of the mutant human insulin receptor of the present invention are boxed in the sequences shown in FIGS. 7 to 11 identical with the sequence represented by SEQ ID NO:1 in Sequence Listing.
The mutant human insulin receptor DNA of the present invention is obtained by amplifying all of the 22 exon genes of the insulin receptor of 51 typical Japanese patients with NIDDM by PCR (polymerase chain reaction) and inserting the PCR products thus amplified into the pUC19 vector followed by DNA sequencing. As the result of the DNA sequencing, three patients had a heterozygous missense mutation Thr.sup.831 .fwdarw.Ala.sup.831 in the exon 13 of the .beta.-subunit and one patient had a heterozygous missense mutation Tyr.sup.1334 .fwdarw.Cys.sup.1334 in the exon 22 of the .beta.-subunit.
The amino acid replacement observed in the former mutation (Thr.sup.831 .fwdarw.Ala.sup.831) has never been reported hitherto. Although this mutation was induced on cDNA and forced to express in animal cells in order to analyze the receptor function, no remarkable disorder in the receptor function was observed. Furthermore, this mutation was not observed in 272 healthy volunteers. Thus statistical analyses indicate that this mutation relates to the onset of NIDDM. To further examine the relationship between this mutation (IR.sup.831) and the onset of NIDDM, the data on a family having the mutant IR.sup.A831 were analyzed. As a result, it is strongly suggested that the heterozygous mutation of IR.sup.A831 would cause the onset of NIDDM.
Similarly, no remarkable disorder in the receptor function is observed in association with the latter mutation (Tyr.sup.1334 .fwdarw.Cys.sup.1334). As the result of analyses of a family, it was considered that this mutation does not relate to the onset of NIDDM. However, the insulin receptor having this mutation cannot bind to PI 3-kinase and the present inventors have recently proven that PI 3-kinase participates in the insulin signal transmission, particularly, translocation of glucose transporter (F. Kanai et al., Biochem. Biophys. Res. Commun. 195:762-768, 1993). Accordingly, it is suggested that this mutation might relate to the onset of NIDDM.
The mutant human insulin receptor DNA of the present invention amounts to at least 6% of the quantitative mutations of the genes responsible for the onset of NIDDM, which is a level significant enough to ensure the application thereof to gene diagnosis of NIDDM. There are a number of genes participating in the onset of NIDDM and mutations in each of these gene have hot spots. It is assumed that several hot spots, including the mutation parts of the above-mention as well as the present invention, would be considered major ones. Supposing that other mutations participating in the onset of NIDDM could be clarified, the combined use thereof with the mutant human insulin receptor DNA of the present invention or its fragment can provide a convenient and reliable method of gene diagnosis for NIDDM. It is expected, furthermore, that the early diagnosis of NIDDM with the combined use of this gene diagnosis and the conventional diagnostic methods largely contributes to the appropriate prevention and treatment of NIDDM.
The mutant human insulin receptor DNA of the present invention, its fragment, DNAs complementary thereto and their fragments, are useful as a diagnostic probe to be used in the above-mentioned diagnosis. The following DNA fragments are usable as the diagnostic probe for NIDDM according to the present invention:
(a) a mutant human insulin receptor DNA fragment wherein the base sequence (ACG) encoding Thr.sup.831 in the exon 13 of the .beta.-subunit of human insulin receptor DNA has been replaced by a base sequence (GCG) encoding Ala;
(b) a mutant human insulin receptor DNA fragment wherein the base sequence (TAC) encoding Tyr.sup.1334 in the exon 22 of the .beta.-subunit of human insulin receptor DNA has been replaced by a base sequence (TGC) encoding Cys; and
(c) a DNA fragment which is complementary to the mutant human insulin receptor DNA fragment of the above (a) or (b).
Such a DNA fragment for a diagnostic probe is generally composed of up to about 100 bases containing the above-mentioned mutation part, preferably from 10 to 50 bases containing the above-mentioned mutation part and still preferably from 10 to 30 bases containing the above-mentioned mutation part.
The DNA or DNA fragment of the present invention can be synthesized in accordance with the base sequence of the present invention by using an automatic DNA synthesizer with the use of, for example, the solid phase method performed on a support such as silica.
The above-mentioned gene diagnosis according to the present invention is not particularly restricted in procedures, etc. and various methods may be selected therefor over a wide range, so long as it aims at detecting the above-mentioned specific mutation(s) characterized by the present invention. Since the gene mutations to be detected by the present invention have been clarified and specified, those skilled in the art can easily select appropriate methods therefor in accordance with the disclosure of the present invention.
For example, this method can be established by analyzing the base sequence of a specific site as defined above, which falls within the scope of the present invention as a matter of course. It therefor seems reasonable to employ Southern hybridization or dot hybridization (each described in Southern, E. M., J. Mol. Biol., 98:503-517, 1975). It is preferable to use a combination of PCR with a gene amplification procedure, since highly sensitive and accurate detection can be conveniently and easily performed with the use of a small amount of DNA specimen. Examples of such a combination include PCR-RFLP (restriction fragment length polymorphism) analysis, PCR-single strand polymorphism analysis (Orita, M., Iwahata, H. Kanazawa, H., Hayashi, K. and Sekiya, T., Proc. Natl. Acad. Sci., USA, 86:2766-2770, 1989), PCR-SSO (specific sequence oligonucleotide) method, PCR-ASO (allele specific oligomer) nucleotide method with the use of dot hybridization (Saiki, R. K., Bugawan, T. L., Horn, G. T., Mullis, K. B. and Erich, H. A, Nature, 324:163-166, 1986), etc.
In the present invention, it is particularly preferable to use the RFLP analysis and/or allele specific hybridization method from the viewpoint of convenience. This detection method will be described in greater detail.
Various operations employed in the detection method of the present invention (for example, chemical synthesis of partial DNA, enzymatic treatments for the cleavage, deletion, addition or binding of DNA, isolation, purification, replication and selection of DNA, etc.) can be each carried out in the conventional manner �Bunshi Idengaku Jikken-ho (Experimental Methods for Molecular Genetics), Kyoritsu Shuppan, 1983; PCR Technology, Takara Shuzo, 1990!. For example, DNA can be isolated and purified by agarose gel electrophoresis, etc. DNA can be sequenced by the dideoxy sequencing method (Sanger, F., Nicklen, S. and Coulson, A. R., Proc. Natl. Acad. Sci., USA, 74:5463-5467, 1977), Maxam-Gilbert method (Maxam, A. M. and Gilbert, W., Method in Enzymology, 65:499-560, 1980), etc. Alternatively, DNA sequencing can be easily carried out by using marketed sequence kits, etc. Also, PCR for amplifying a specific region of DNA can be carried out in accordance with the conventional method (see, for example, Saiki, R. K., Scharf, S., Faloona, F. A., Mullis, K. B., Horn, G. T., Erlich, H. A. and Arnheim, N., Science, 230:1350-1354, 1985). These fundamental operations are employed in, for example, the references cited herein which are provided by way of reference similar to the Examples presented hereinbelow.
The genomic DNA to be assayed by the detection method of the present invention may be obtained from any source, so long as it is a sample originating in a human being and containing the genomic DNA. The genomic DNA can be extracted and purified from such a sample in accordance with the conventional method.
From this genomic DNA, a DNA region containing the mutation site relating to the present invention is amplified. Thus a concentrated specimen can be obtained in a large amount. For example, PCR can be performed with the use of appropriate primers by which a region containing the above-mentioned mutation in the exon 13 or 22 alone can be specifically amplified. These primers can be selected in the conventional manner. The base length of the region to be amplified is not particularly restricted but usually regulated to 100 to 500 bp. It is proper to employ, for example, primers homologous to the flanking intronic sequence (Seino, S., Seino, M. and Bell, Gl., Diabetes, 39:123-128, 1990) which were employed in Examples given hereinafter in order to amplify a region including the exon 13. To amplify a region including the exon 22, use can be made of, for example, a sense primer (5'-CACTGACCTCATGCGCATGTGCTGG-3') (SEQ ID NO:3) and an antisense primer (5'-ATTGGACCGAGGCAAGGTCAGAAT-3') (SEQ ID NO:4) are employed. By using these primers, the desired regions as described above can be obtained as amplified DNA fragments of 322 bp (exon 13) and 346 bp (exon 22) respectively.
By using the desired DNA region which has been amplified by PCR, the specific mutation of the present invention contained in this region can be detected and confirmed. In the Examples given hereinafter, the mutation in the exon 13 was detected by the RFLP method. The mutation at Ala.sup.831 (GCG) results in the specific cleavage site of the restriction enzyme CfoI. When the above-mentioned PCR amplification product of the exon 13 (322 bp) having this mutation is digested with CfoI, therefore, two fragments (102 bp, 220 bp) are obtained. In contrast, no such cleavage occurs in the mutation-free wild type (322 bp). The fragments thus formed are identified as specific bands by the conventional method.
To detect the mutation in the exon 22, use is made of allele-specific hybridization wherein the above-mentioned DNA fragment for diagnostic probe is employed. This hybridization can be performed in a conventional manner, so long as it aims at detecting the mutation as specified in the present invention with the use of the specific diagnostic probe as described above. For example, the following conditions were employed in Examples given hereinafter.
The PCR amplification product (346 bp) of the exon 22 as described above, which had been transferred onto a nitrocellulose filter, was hybridized overnight at 30.degree. C. in a probe solution containing 6.times. SSC, 10.times. Denhardt's solution, 1% of SDS, 1 mg/ml of salmon sperm DNA and a .sup.32 P-labeled probe. The hybridized filter was washed twice with 6.times. SSC in 0.1% of SDS at 54.degree. C. each for 20 minutes and then autoradio-graphed. The probe specific to the IR.sup.C1334 mutation (Cys.sup.1334 :TGC), by which it is distinguishable from the wild type (Tyr.sup.1334 :TAC), employed herein was the .sup.32 P-labeled probe 5'-ATGTGTGTGCAAGGGATGT-3' (SEQ ID NO:5).
The occurrence of the mutation of the present invention can be detected by observing the pattern of the bands thus obtained (two hybridized bands of 102 bp and 220 bp assignable to the mutation in the exon 13, or one of 346 bp assignable to the mutation in the exon 22) and confirming the same.
In this gene diagnosis, it is advantageous to utilize a diagnostic drug which contains as the active ingredient a means or reagent for detecting the occurrence of the mutation according to the present invention. Therefore, the present invention further provides such a drug for the diagnosis of non-insulin-dependent diabetes mellitus. This diagnostic drug contains as the essential ingredient a specific reagent suitable for the method for detecting the occurrence of the mutation of the present invention. This specific reagent may be appropriately selected depending on the employed detection method. It is characterized by being necessary in the means for specifically detecting the mutation of the present invention, for example, the DNA fragment for diagnostic probe as described above and/or a specific restriction enzyme. Although a reagent for PCR-amplifying specifically a region of the mutation of the present invention (for example, a primer designed therefor, etc.) cannot be regarded as the essential ingredient of the diagnostic drug of the present invention, the diagnostic drug of the present invention may contain such a reagent together with the reagent(s) for hybridization.
To further illustrate the present invention in greater detail, and not by way of limitation, the following Examples will be given.
EXAMPLES
Example 1
Separation of human chromosomal DNA
Ten ml portions of blood of about 100 typical Japanese patients with NIDDM were provided by Dr. Makino et al. of the Second Department of Internal Medicine, Faculty of Medicine, Chiba University. Human chromosomal DNA was separated in the following manner.
1) Into two 50 ml blue-capped tubes were introduced 45 ml portions of solution A �0.32 M of sucrose, 10 mM of Tris-HCl (pH 7.5), 5 mM of MgCl.sub.2 and 1% of Triton X-100! with a 10 ml graduated pipet.
2) The blood was collected in an amount of about 10 ml.
3) About 5 ml portions of the blood were added to the two blue-capped tubes containing the solution A followed by end-over-end mixing.
4) Each mixture was centrifuged at 4.degree. C. for 10 minutes at 3,000 rpm.
5) The supernatant was carefully discarded and the tube was placed upside-down on Kim-Wipe to thereby eliminate the solution.
6) Into this tube was introduced 4 ml of solution B �0.075 M of NaCl, 0.024 M of EDTA (pH 8.0)! with a 5 ml graduated pipet. After mixing, pellets were peeled off from the bottom of the tube, transferred into another tube and mixed well with a vortex mixer.
7) To this mixture was added 1 ml of solution C (containing equivalent amounts of 5% of SDS and 2 mg/ml of proteinase K) with an automatic pipet. After mixing well, the mixture was reacted overnight at 37.degree. C.
8) Five ml of a phenol solution was added thereto with a 5 ml graduated pipet and, after capping, mixed well. Further, 5 ml of a mixture of chloroform with isoamyl alcohol (24:1) was added thereto and, after capping, mixed well.
9) The whole solution was transferred into an orange-capped conical tube (15 ml) and centrifuged at 3,000 rpm for 10 minutes.
10) The supernatant was taken up carefully with a Pasteur pipet having a cut-off tip and transferred into a fresh orange-capped tube. Then 5 ml of a mixture of phenol with chloroform (1:1) was added thereto and mixed for 30 minutes.
11) The mixture was centrifuged at 3,000 rpm for 10 minutes.
12) The supernatant was taken up carefully with a Pasteur pipet having a cut-off tip and transferred into a blue-capped tube (50 ml).
13) After adding 0.5 ml of solution D (3 M sodium acetate) with an automatic pipet, capping and mixing, 10 ml of cold ethanol (99.9%) was slowly layered thereon with a graduated pipet.
14) After capping, the mixture was slowly mixed end-over-end. Thus, the chromosomal DNA was obtained as a white insoluble matter. It was scooped up with a Pasteur pipet having a bowed tip and gently immersed in 1 ml of a 70% ethanol solution for about 15 seconds. Next, the insoluble matter was transferred into an Eppendorf tube containing 200 .mu.l of solution E �10 mM of Tris-HCl (pH 7.5), 1 mM of EDTA).
15) The Eppendorf tube was capped and the contents were mixed with a vortex mixer several times to thereby well dissolve the DNA.
Similarly, chromosomal DNA was separated from 272 healthy volunteers.
Example 2
Amplification of insulin receptor gene exons by PCR, subcloning thereof and isolation of plasmid DNA
In accordance with the method of Seino et al. (Proc. Natl. Acad. Sci. USA 86:114-118, 1989; Diabetes 39:123-128, 1990), the insulin receptor gene was amplified by PCR with the use of primer DNAs by which all of 22 exons of the insulin receptor gene could be amplified.
Namely, 99 .mu.l of mixed solution F of the following composition was introduced into a 0.5 ml tube. Next, 1 .mu.l (1 .mu.g) of the DNA was added thereto and mixed therewith.
______________________________________(Volume) (Final)______________________________________10 x reaction buffer 10.0 .mu.l 1 xdNTPs mix (2.5 mM) 8.0 .mu.l 200 .mu.Mupstream primer 0.5 .mu.l 50 pmol/100 .mu.ldownstream primer 0.5 .mu.l 50 pmol/100 .mu.lH.sub.2 O 79.5 .mu.lAmpli Taq .TM. polymerase 0.5 .mu.l 2.5 U/assaytotal 99.0 .mu.l.______________________________________
Mineral oil was layered onto the above mixture so as to prevent the sample from evaporating. To improve the heat conductivity, a drop of mineral oil was further added to each well of the heat box. By using them, PCR was performed under the conditions as specified below.
______________________________________1. Initial denaturation 94.degree. C., 3 minutes.2. Denaturation 94.degree. C., 1 minute.3. Annealing 53.degree. C., 1.5 minutes .times. 30 cycles.4. Extension 72.degree. C., 2.5 minutes, terminated at 72.degree. C., 4 minutes.______________________________________
To the sample thus amplified by PCR was added 100 .mu.l of chloroform. After mixing well with a vortex mixer, the mixture was centrifuged for 1 minute. Then the lower layer was thoroughly eliminated with a Pasteur pipet and DNA was obtained from the upper layer.
The PCR amplified DNA fragments thus obtained were purified by agarose gel electrophoresis and ligated to the HincII site of pUC19 which had been treated with alkaline phosphatase. Then these recombinants were introduced into Escherichia coli. From several hundred colonies tolerant to ampicillin, 12 colonies were picked up. Then it was thus confirmed for each exon that more than 50% of the colonies had a PCR DNA fragment. All of the remaining colonies were scratched off from the agar medium and grown in a liquid medium. Then crude plasmid DNAs were separated by the alkaline lysis method and RNAs were removed by precipitation with polyethylene glycol. It is considered that, when a number of E. coli transformants are used as in the case of the present Example, the patroclinal PCR products and the matroclinal ones are obtained almost in the same amount. Accordingly, the base sequences of the DNAs thus separated have both of the patroclinal and matroclinal gene sequences therein.
Example 3
DNA sequencing
The DNA fragments obtained in Example 2 were sequenced by the dideoxy sequencing method with the use of in isotope (Maxam, A. M. and Gilbert, W., Proc. Natl. Acad. Sci. USA 74:560-564, 1977). In brief, the primers employed in the DNA amplification by PCR were hybridized with the DNA fragments. Then DNAs were synthesized with Sequenase by using isotope-labeled dCTP, followed by electrophoresis and autoradiography.
As a result, mutations free from any amino acid replacement were found in ten patients while mutations accompanied by amino acid replacement were found in four patients.
Among the four patients with mutations accompanied by amino acid replacement as described above, three patients showed the mutation (Thr.sup.831 .fwdarw.Ala.sup.831) in the exon 13 of the insulin receptor .beta.-subunit, as shown below.
______________________________________Val.sup.830 - Thr.sup.831 - His.sup.832GTGmal 5'ACGCAT3'CAC 3'TGCGTA5'GTGant 5'GCGCAT3'CAC 3'CGCGTA5'Val.sup.830 - Ala.sup.831 - His.sup.832.______________________________________
FIG. 1 is an electrophorogram which shows a part of the DNA base sequence of the exon 13 of the insulin receptor .beta.-subunit of five patients with NIDDM involving those having the above mutation. It was found that the patient of the third lane was a heterozygote having both of the sequences Thr.sup.831 (ACG) and Ala.sup.831 (GCG). Two other patients had the same sequences.
On the other hand, the remaining patient having mutation accompanied with amino acid replacement showed the following mutation (Tyr.sup.1334 .fwdarw.Cys.sup.1334) in the exon 22 of the insulin receptor .beta.-subunit, as shown below.
______________________________________Pro.sup.1333 - Tyr.sup.1334 - Thr.sup.1335CCTmal 5'TACACA3'GGA 3'ATGTGT5'CCTant 5'TGCACA3'GGA 3'ACGTGT5'Pro.sup.1333 - Cys.sup.1334 - Thr.sup.1335.______________________________________
FIG. 2 is an electrophorogram which shows a part of the DNA base sequence of the exon 22 of the insulin receptor .beta.-subunit of eight patients with NIDDM involving the one having the above mutation. It was found that the patient of the seventh lane was a heterozygote having both of the sequences Tyr.sup.1334 (TAC) and Cys.sup.1334 (TGC).
Example 4
Functional characterization of mutant insulin receptors expressed in mammalian cells
Test Method
(1) Construction of expression plasmids
By using PCR on cDNA, two artificially mutated cDNAs, i.e., insulin receptors IR.sup.A831 having the mutation (Thr.sup.831 .fwdarw.Ala.sup.831) and IR.sup.C1334 having the mutation (Tyr.sup.1334 .fwdarw.Cys.sup.1334) were constructed.
Next, the artificial mutant cDNAs IR.sup.A831 and IR.sup.C1334 were subcloned into a mammalian expression vector SR.alpha. (Y. Tanabe et al., Mol. Cell Biol. 8:446-472, 1988) to thereby give SR.alpha.IR.sup.A831 and SR.alpha.IR.sup.C1334 respectively. The wild type insulin receptor SR.alpha.IR.sup.WT employed as the control was constructed by a method of F. Kanai et al. (J. Bio. Chem. 268:14523-14526, 1993).
(2) Establishment of CHO cells expressing the wild type and artificial mutant insulin receptors
CHO cells were transfected with SR.alpha.IR.sup.WT, SR.alpha.IR.sup.A831 or SR.alpha.IR.sup.C1334 (each 10 .mu.g) and pSV2-neo (1 .mu.g). After selecting with 400 .mu.g/ml of G418 (Sigma), cells expressing human insulin receptors were identified by .sup.125 I-labeled insulin binding in accordance with the method of H. Hayashi et al. (Biochem. J. 280:769-775, 1991). The number of cell surface receptors was calculated by Scatchard analysis (G. Scathard, Ann. NY Acad. Sci. 51:660-672, 1949).
(3) Assay of receptor tyrosine kinase activities
By using the method of Hayashi et al. (Biochem. J. 280:769-775, 1991), the insulin-stimulated receptor autophosphorylation of IR.sup.WT and IR.sup.A831 was performed in a 96-well plate. The incorporation of .sup.32 P into the receptor .beta.-subunit was detected by 6% SDS-PAGE and measured by a Bio-image-analyzer BAS2000.
(4) Insulin-induced complex formation of insulin receptors and .alpha.-type p85 subunit of PI 3-kinase.
To transiently express both of the .alpha.-type p85 subunit of PI 3-kinase and wild type or mutant IRs, COS-7 cells were transfected with SR.alpha.p85.alpha. (1.5 .mu.g) and SR.alpha.IR, SR.alpha.IR.sup.A831 or SR.alpha.IR.sup.C1334 (each 1.5 .mu.g), respectively by using Lipofectamine.TM. Reagent (Bethesda Research Laboratories). After stimulating with 10.sup.-7 M of insulin for 10 minutes, cell lysates were prepared and incubated with an anti-IR antibody 1G2, which recognizes the .beta.-subunit of IR, or a rabbit polyclonal anti-p85.alpha. antibody, and protein G-Sepharose. The immunoprecipitates were electrophoresed on a 6% SDS-PAGE. Immunoblotting was performed by using an anti-phosphotyrosine antibody (PY20) or an anti-insulin receptor antibody 3B11 which recognizes the .alpha.-subunit of IR.
Results
(1) A831 mutation
Examination was made on two clones stably expressing IR.sup.WT (clone Nos. 12 and 21) and two clones stably expressing IR.sup.A831 (clone Nos. 10 and 17) obtained in the above test method (2).
FIG. 3A shows the Scatchard analysis of IR.sup.WT (clone Nos. 12 and 21) and IR.sup.A831 (clone Nos. 10 and 17). The number of insulin binding sites in each clone were counted. As a result, the number of high affinity binding sites were 1.1 and a.5.times.10.sup.6 per cell in IR.sup.WT and 0.5 and 0.9.times.10.sup.9 per cell in IR.sup.A831, and the dissociation constants (Kd) were 2.8 and 3.5 nM for IR.sup.WT and 1.7 and 2.7 nM for IR.sup.A831. IR.sup.WT and IR.sup.A831 showed each the occurrence of dissociation with a decrease in pH (from 7.5 to 5.5) and no difference was observed between the wild type and the mutant IRs.
Next, the autophosphorylation activities of these receptors were determined by the test method (3) described above. FIG. 3B shows the results thus obtained. As FIG. 3B clearly shows, the activities of receptor autophosphorylation were increased in association with the receptor numbers. Half-maximal stimulation occurred at 5.5 and 5.4.times.10.sup.-10 M for IR.sup.WT and at 5.0 and 4.9.times.10.sup.-10 for IR.sup.A831, showing no remarkable difference between the wild type and the mutant IRs.
Further, the tyrosine kinase activity toward an endogenous substrate IRS-1 was determined by immunoblotting with the use of an anti-phosphotyrosine antibody (PY-20). FIG. 3C shows the results. As FIG. 3C clearly shows, the IRS-1 (molecular weight: 160,000) was phosphorylated insulin-dependently at tyrosine residues in parallel to the autophosphorylation rate of the receptor .beta.-subunit (molecular weight: 95,000).
Subsequently, the insulin binding and receptor autophosphorylation were examined by using COS cells transiently expressing IR.sup.WT and IR.sup.A831 so as to avoid differences among CHO clones stably expressing each IR. No remarkable difference was observed between the wild type and artificial mutant receptors in either insulin binding affinity or in receptor kinase activity. Similarly, no distinct difference was observed between the wild type and artificial mutant receptors in receptor processing, internalization, degradation, insulin-stimulated glucose uptake, glycogen synthesis and DNA synthesis. That is to say, no direct evidence was found regarding the receptor function disorders in the A831 mutation.
(2) C1334 mutation
The above-mentioned experiments performed on IR.sup.A831 were repeated on IR.sup.C1334 to thereby examine the receptor function disorders in IR.sup.C1334.
No distinct difference in the functions between IR.sup.C1334 and RI.sup.WT was found out in the examination with the use of CHO clones stably expressing IRs.
However, a difference was observed in the affinity to PI 3-kinase. It is known that the autophosphorylated Tyr.sup.1334 of IR locates in the putative binding motif �Y(P)XXM! to the SH2 domains of the 85-kDa regulatory subunit (p85) of phosphatidylinositol 3-kinase (D. J. Van Horn et al., J. Biol. Chem. 269:29-32, 1994). Since the binding of autophosphorylated IR to PI 3-kinase in response to insulin leads to the activation of this enzyme, this mechanism might be an alternative pathway for the activation of PI 3-kinase by the binding of IRS-1. The present inventors had formerly reported that PI 3-kinase mediates the translocation of glucose transporter type 4 (GLUT4) (see, for example, F. Kanai et al., Biochem. Biophys. Res. Commun. 195:762-768, 1993). Thus examination was made on the direct interactions between IR.sup.C1334 and p85 of PI 3-kinase.
First, the p85 subunit of PI 3-kinase was transiently expressed in COS cells with the use of IR.sup.WT, IR.sup.A831 and IR.sup.C1334. After treating with insulin, the cell lysates were precipitated by either an anti-IR.beta. antibody (.alpha.IR.beta.) or an anti-p85 antibody (.alpha.p85) (FIG. 4). The immunoprecipitates were examined by immunoblotting with an anti-phosphotyrosine antibody (.alpha.PY) (FIG. 4A) or an anti-IR .alpha.-subunit antibody (.alpha.IR.alpha.) (FIG. 4B). In the transient expression system, the insulin treatment stimulated the tyrosine phosphorylation of the p85 subunit of PI 3-kinase and the binding of the p85 to IR.sup.WT. When immunoblotted with anti-IR.alpha. antibody, the anti-IR.beta. antibody precipitated IR.sup.WT,IR A831 and IR.sup.C1334 to almost the same degree (FIG. 4B). When immunoblotted with anti-p85 antibody, the anti-p85 antibody precipitated IR.sup.WT, IR.sup.A831 and IR.sup.C1334 to almost the same degree.
Subsequently, examination was made on the tyrosine phosphorylation of p85 and IR.beta., and the binding of p85 to IR.sup.WT, IR.sup.A831 and IR.sup.C1334. When immunoblotted with anti-phosphotyrosine antibody (FIG. 4A), all of the IRs phosphorylated p85 at tyrosine residues to the same extent. The tyrosine phosphorylation of IR.sup.C1334 was reduced compared with IR.sup.WT and IR.sup.A831 (FIG. 4A). This reduction did not relate to the decrease in the tyrosine kinase activity of IR.sup.C1334. In the examination on the incorporation of .sup.32 P into the substrates, IR.sup.C1334 showed almost the same activity toward the autophosphorylation sites and an exogenous substrate poly(Glu, Tyr) 4:1 as IR.sup.WT and IR.sup.A831 in CHO cells expressing these IRs. Thus it is considered that the antiphosphotyrosine antibody would preferably recognize the site of autophosphorylated Y.sup.1334 more than other autophosphorylation sites of IR.beta.. The anti-p85 antibody co-precipitated with IR.sup.WT and IR.sup.A831 but not with IR.sup.C1334 (FIG. 4B). This fact means that IR.sup.C1334 would not bind to p85 and that the major binding site of IR to p85 might be the Y.sup.1334 residue.
Example 5
Statistical Processing
272 healthy volunteers were analyzed to find those having the mutant insulin receptor DNA (Thr.sup.831 .fwdarw.Ala.sup.831). As a result, none of the volunteers showed the mutation. Thus, the relation between the mutant insulin receptor DNA of the present invention and NIDDM was tested through statistical processing by chi-square analysis. The results are given in the following Table 1.
TABLE 1______________________________________Analysis on the frequency of the occurrence of insulinreceptor .beta.-subunit mutation (T.sup.831 .fwdarw. A.sup.831)in NIDDM and non-diabetic subjectsNIDDM Non-diabetic.sup.1) Total______________________________________A.sup.831 3.sup.2) 0 3T.sup.831 48 272 320Total 51 272 323______________________________________ .sup.1) The nondiabetic subjects were not examined by OGTT, etc. but selected by inquiring whether they had any medical history or family histories of diabetes in their immediate parents or grandparents. .sup.2) The mutation (Thr.sup.831 .fwdarw. Ala.sup.831) significantly related to the onset of NIDDM in the chisquare analysis with Yates correction (p < 0.05). Supposing that the spontaneous onset rate of NIDDM is 5% and 5% (14 subjects) of the 272 nondiabetic subjects might migrate into the NIDDM T.sup.831 group, the mutation still shows a significant difference in the above calibration.
Example 6
Pedigree-linkage Analysis
(1) Relationship between IR.sup.C133 and the onset of NIDDM
To examine the relationship between IR.sup.C1334 and the onset of NIDDM, data on the family with IR.sup.C1334 were analyzed.
In this family, the mother and the second son were reported to be diabetics, and the other two children (T. S. and S. K.) in addition to the proband (Y. Y.) were diagnosed as diabetics (FIG. 5A). When examined by the allele specific hybridization at TAC (Tyr.sup.1334).fwdarw.TGC (Cys.sup.1334) (FIG. 5B), however, two of them (T. S. and S. K.) had the normal IR (IR.sup.Y1334) while the proband alone had IR.sup.C1334. Thus the results of this pedigree-linkage analysis indicate that the mutant IR.sup.C1334 is not the common cause of the onset of NIDDM in this family.
(2) Relationship between IR.sup.A831 and the onset of NIDDM
To examine the relationship between IR.sup.A831 and the onset of NIDDM, data on the family with IR.sup.A831 were analyzed. The deceased proband's father had NIDDM, but his mutation could not be confirmed. The mother could not be subjected to the examination due to her advanced age. All four siblings were heterozygotes of IR.sup.A831, and three had NIDDM while one had an impaired glucose tolerance (IGT), i.e., intermediate between normal and diabetes (FIG. 6). IR.sup.A831 was detected by the above-mentioned method with the formation of the specific cleavage site of the restriction enzyme CfoI (FIG. 6B). Consanguineous marriage in the family was ruled out. The allele of IR.sup.A831 may have been derived from the father. This pedigree and linkage analysis data strongly suggest that the heterozygous mutation IR.sup.A831 would be responsible for the onset of NIDDM.
__________________________________________________________________________# SEQUENCE LISTING- (1) GENERAL INFORMATION:- (iii) NUMBER OF SEQUENCES: 5- (2) INFORMATION FOR SEQ ID NO:1:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 4149 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: cDNA- (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..4146- (ix) FEATURE: (A) NAME/KEY: mat.sub.-- - #peptide (B) LOCATION: 82..4146- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:- ATG GGC ACC GGG GGC CGG CGG GGG GCG GCG GC - #C GCG CCG CTG CTG GTG 48Met Gly Thr Gly Gly Arg Arg Gly Ala Ala Al - #a Ala Pro Leu Leu Val15- GCG GTG GCC GCG CTG CTA CTG GGC GCC GCG GG - #C CAC CTG TAC CCC GGA 96Ala Val Ala Ala Leu Leu Leu Gly Ala Ala Gl - #y His Leu Tyr Pro Gly# 5 1- GAG GTG TGT CCC GGC ATG GAT ATC CGG AAC AA - #C CTC ACT AGG TTG CAT 144Glu Val Cys Pro Gly Met Asp Ile Arg Asn As - #n Leu Thr Arg Leu His# 20- GAG CTG GAG AAT TGC TCT GTC ATC GAA GGA CA - #C TTG CAG ATA CTC TTG 192Glu Leu Glu Asn Cys Ser Val Ile Glu Gly Hi - #s Leu Gln Ile Leu Leu# 35- ATG TTC AAA ACG AGG CCC GAA GAC TTC CGA GA - #C CTC AGT TTC CCC AAA 240Met Phe Lys Thr Arg Pro Glu Asp Phe Arg As - #p Leu Ser Phe Pro Lys# 50- CTC ATC ATG ATC ACT GAT TAC TTG CTG CTC TT - #C CGG GTC TAT GGG CTC 288Leu Ile Met Ile Thr Asp Tyr Leu Leu Leu Ph - #e Arg Val Tyr Gly Leu# 65- GAG AGC CTG AAG GAC CTG TTC CCC AAC CTC AC - #G GTC ATC CGG GGA TCA 336Glu Ser Leu Lys Asp Leu Phe Pro Asn Leu Th - #r Val Ile Arg Gly Ser# 85- CGA CTG TTC TTT AAC TAC GCG CTG GTC ATC TT - #C GAG ATG GTT CAC CTC 384Arg Leu Phe Phe Asn Tyr Ala Leu Val Ile Ph - #e Glu Met Val His Leu# 100- AAG GAA CTC GGC CTC TAC AAC CTG ATG AAC AT - #C ACC CGG GGT TCT GTC 432Lys Glu Leu Gly Leu Tyr Asn Leu Met Asn Il - #e Thr Arg Gly Ser Val# 115- CGC ATC GAG AAG AAC AAT GAG CTC TGT TAC TT - #G GCC ACT ATC GAC TGG 480Arg Ile Glu Lys Asn Asn Glu Leu Cys Tyr Le - #u Ala Thr Ile Asp Trp# 130- TCC CGT ATC CTG GAT TCC GTG GAG GAT AAT CA - #C ATC GTG TTG AAC AAA 528Ser Arg Ile Leu Asp Ser Val Glu Asp Asn Hi - #s Ile Val Leu Asn Lys# 145- GAT GAC AAC GAG GAG TGT GGA GAC ATC TGT CC - #G GGT ACC GCG AAG GGC 576Asp Asp Asn Glu Glu Cys Gly Asp Ile Cys Pr - #o Gly Thr Ala Lys Gly150 1 - #55 1 - #60 1 -#65- AAG ACC AAC TGC CCC GCC ACC GTC ATC AAC GG - #G CAG TTT GTC GAA CGA 624Lys Thr Asn Cys Pro Ala Thr Val Ile Asn Gl - #y Gln Phe Val Glu Arg# 180- TGT TGG ACT CAT AGT CAC TGC CAG AAA GTT TG - #C CCG ACC ATC TGT AAG 672Cys Trp Thr His Ser His Cys Gln Lys Val Cy - #s Pro Thr Ile Cys Lys# 195- TCA CAC GGC TGC ACC GCC GAA GGC CTC TGT TG - #C CAC AGC GAG TGC CTG 720Ser His Gly Cys Thr Ala Glu Gly Leu Cys Cy - #s His Ser Glu Cys Leu# 210- GGC AAC TGT TCT CAG CCC GAC GAC CCC ACC AA - #G TGC GTG GCC TGC CGC 768Gly Asn Cys Ser Gln Pro Asp Asp Pro Thr Ly - #s Cys Val Ala Cys Arg# 225- AAC TTC TAC CTG GAC GGC AGG TGT GTG GAG AC - #C TGC CCG CCC CCG TAC 816Asn Phe Tyr Leu Asp Gly Arg Cys Val Glu Th - #r Cys Pro Pro Pro Tyr230 2 - #35 2 - #40 2 -#45- TAC CAC TTC CAG GAC TGG CGC TGT GTG AAC TT - #C AGC TTC TGC CAG GAC 864Tyr His Phe Gln Asp Trp Arg Cys Val Asn Ph - #e Ser Phe Cys Gln Asp# 260- CTG CAC CAC AAA TGC AAG AAC TCG CGG AGG CA - #G GGC TGC CAC CAA TAC 912Leu His His Lys Cys Lys Asn Ser Arg Arg Gl - #n Gly Cys His Gln Tyr# 275- GTC ATT CAC AAC AAC AAG TGC ATC CCT GAG TG - #T CCC TCC GGG TAC ACG 960Val Ile His Asn Asn Lys Cys Ile Pro Glu Cy - #s Pro Ser Gly Tyr Thr# 290- ATG AAT TCC AGC AAC TTG CTG TGC ACC CCA TG - #C CTG GGT CCC TGT CCC1008Met Asn Ser Ser Asn Leu Leu Cys Thr Pro Cy - #s Leu Gly Pro Cys Pro# 305- AAG GTG TGC CAC CTC CTA GAA GGC GAG AAG AC - #C ATC GAC TCG GTG ACG1056Lys Val Cys His Leu Leu Glu Gly Glu Lys Th - #r Ile Asp Ser Val Thr310 3 - #15 3 - #20 3 -#25- TCT GCC CAG GAG CTC CGA GGA TGC ACC GTC AT - #C AAC GGG AGT CTG ATC1104Ser Ala Gln Glu Leu Arg Gly Cys Thr Val Il - #e Asn Gly Ser Leu Ile# 340- ATC AAC ATT CGA GGA GGC AAC AAT CTG GCA GC - #T GAG CTA GAA GCC AAC1152Ile Asn Ile Arg Gly Gly Asn Asn Leu Ala Al - #a Glu Leu Glu Ala Asn# 355- CTC GGC CTC ATT GAA GAA ATT TCA GGG TAT CT - #A AAA ATC CGC CGA TCC1200Leu Gly Leu Ile Glu Glu Ile Ser Gly Tyr Le - #u Lys Ile Arg Arg Ser# 370- TAC GCT CTG GTG TCA CTT TCC TTC TTC CGG AA - #G TTA CGT CTG ATT CGA1248Tyr Ala Leu Val Ser Leu Ser Phe Phe Arg Ly - #s Leu Arg Leu Ile Arg# 385- GGA GAG ACC TTG GAA ATT GGG AAC TAC TCC TT - #C TAT GCC TTG GAC AAC1296Gly Glu Thr Leu Glu Ile Gly Asn Tyr Ser Ph - #e Tyr Ala Leu Asp Asn390 3 - #95 4 - #00 4 -#05- CAG AAC CTA AGG CAG CTC TGG GAC TGG AGC AA - #A CAC AAC CTC ACC ACC1344Gln Asn Leu Arg Gln Leu Trp Asp Trp Ser Ly - #s His Asn Leu Thr Thr# 420- ACT CAG GGG AAA CTC TTC TTC CAC TAT AAC CC - #C AAA CTC TGC TTG TCA1392Thr Gln Gly Lys Leu Phe Phe His Tyr Asn Pr - #o Lys Leu Cys Leu Ser# 435- GAA ATC CAC AAG ATG GAA GAA GTT TCA GGA AC - #C AAG GGG CGC CAG GAG1440Glu Ile His Lys Met Glu Glu Val Ser Gly Th - #r Lys Gly Arg Gln Glu# 450- AGA AAC GAC ATT GCC CTG AAG ACC AAT GGG GA - #C AAG GCA TCC TGT GAA1488Arg Asn Asp Ile Ala Leu Lys Thr Asn Gly As - #p Lys Ala Ser Cys Glu# 465- AAT GAG TTA CTT AAA TTT TCT TAC ATT CGG AC - #A TCT TTT GAC AAG ATC1536Asn Glu Leu Leu Lys Phe Ser Tyr Ile Arg Th - #r Ser Phe Asp Lys Ile470 4 - #75 4 - #80 4 -#85- TTG CTG AGA TGG GAG CCG TAC TGG CCC CCC GA - #C TTC CGA GAC CTC TTG1584Leu Leu Arg Trp Glu Pro Tyr Trp Pro Pro As - #p Phe Arg Asp Leu Leu# 500- GGG TTC ATG CTG TTC TAC AAA GAG GCC CCT TA - #T CAG AAT GTG ACG GAG1632Gly Phe Met Leu Phe Tyr Lys Glu Ala Pro Ty - #r Gln Asn Val Thr Glu# 515- TTC GAT GGG CAG GAT GCG TGT GGT TCC AAC AG - #T TGG ACG GTG GTA GAC1680Phe Asp Gly Gln Asp Ala Cys Gly Ser Asn Se - #r Trp Thr Val Val Asp# 530- ATT GAC CCA CCC CTG AGG TCC AAC GAC CCC AA - #A TCA CAG AAC CAC CCA1728Ile Asp Pro Pro Leu Arg Ser Asn Asp Pro Ly - #s Ser Gln Asn His Pro# 545- GGG TGG CTG ATG CGG GGT CTC AAG CCC TGG AC - #C CAG TAT GCC ATC TTT1776Gly Trp Leu Met Arg Gly Leu Lys Pro Trp Th - #r Gln Tyr Ala Ile Phe550 5 - #55 5 - #60 5 -#65- GTG AAG ACC CTG GTC ACC TTT TCG GAT GAA CG - #C CGG ACC TAT GGG GCC1824Val Lys Thr Leu Val Thr Phe Ser Asp Glu Ar - #g Arg Thr Tyr Gly Ala# 580- AAG AGT GAC ATC ATT TAT GTC CAG ACA GAT GC - #C ACC AAC CCC TCT GTG1872Lys Ser Asp Ile Ile Tyr Val Gln Thr Asp Al - #a Thr Asn Pro Ser Val# 595- CCC CTG GAT CCA ATC TCA GTG TCT AAC TCA TC - #A TCC CAG ATT ATT CTG1920Pro Leu Asp Pro Ile Ser Val Ser Asn Ser Se - #r Ser Gln Ile Ile Leu# 610- AAG TGG AAA CCA CCC TCC GAC CCC AAT GGC AA - #C ATC ACC CAC TAC CTG1968Lys Trp Lys Pro Pro Ser Asp Pro Asn Gly As - #n Ile Thr His Tyr Leu# 625- GTT TTC TGG GAG AGG CAG GCG GAA GAC AGT GA - #G CTG TTC GAG CTG GAT2016Val Phe Trp Glu Arg Gln Ala Glu Asp Ser Gl - #u Leu Phe Glu Leu Asp630 6 - #35 6 - #40 6 -#45- TAT TGC CTC AAA GGG CTG AAG CTG CCC TCG AG - #G ACC TGG TCT CCA CCA2064Tyr Cys Leu Lys Gly Leu Lys Leu Pro Ser Ar - #g Thr Trp Ser Pro Pro# 660- TTC GAG TCT GAA GAT TCT CAG AAG CAC AAC CA - #G AGT GAG TAT GAG GAT2112Phe Glu Ser Glu Asp Ser Gln Lys His Asn Gl - #n Ser Glu Tyr Glu Asp# 675- TCG GCC GGC GAA TGC TGC TCC TGT CCA AAG AC - #A GAC TCT CAG ATC CTG2160Ser Ala Gly Glu Cys Cys Ser Cys Pro Lys Th - #r Asp Ser Gln Ile Leu# 690- AAG GAG CTG GAG GAG TCC TCG TTT AGG AAG AC - #G TTT GAG GAT TAC CTG2208Lys Glu Leu Glu Glu Ser Ser Phe Arg Lys Th - #r Phe Glu Asp Tyr Leu# 705- CAC AAC GTG GTT TTC GTC CCC AGA AAA ACC TC - #T TCA GGC ACT GGT GCC2256His Asn Val Val Phe Val Pro Arg Lys Thr Se - #r Ser Gly Thr Gly Ala710 7 - #15 7 - #20 7 -#25- GAG GAC CCT AGG CCA TCT CGG AAA CGC AGG TC - #C CTT GGC GAT GTT GGG2304Glu Asp Pro Arg Pro Ser Arg Lys Arg Arg Se - #r Leu Gly Asp Val Gly# 740- AAT GTG ACG GTG GCC GTG CCC ACG GTG GCA GC - #T TTC CCC AAC ACT TCC2352Asn Val Thr Val Ala Val Pro Thr Val Ala Al - #a Phe Pro Asn Thr Ser# 755- TCG ACC AGC GTG CCC ACG AGT CCG GAG GAG CA - #C AGG CCT TTT GAG AAG2400Ser Thr Ser Val Pro Thr Ser Pro Glu Glu Hi - #s Arg Pro Phe Glu Lys# 770- GTG GTG AAC AAG GAG TCG CTG GTC ATC TCC GG - #C TTG CGA CAC TTC ACG2448Val Val Asn Lys Glu Ser Leu Val Ile Ser Gl - #y Leu Arg His Phe Thr# 785- GGC TAT CGC ATC GAG CTG CAG GCT TGC AAC CA - #G GAC ACC CCT GAG GAA2496Gly Tyr Arg Ile Glu Leu Gln Ala Cys Asn Gl - #n Asp Thr Pro Glu Glu790 7 - #95 8 - #00 8 -#05- CGG TGC AGT GTG GCA GCC TAC GTC AGT GCG AG - #G ACC ATG CCT GAA GCC2544Arg Cys Ser Val Ala Ala Tyr Val Ser Ala Ar - #g Thr Met Pro Glu Ala# 820- AAG GCT GAT GAC ATT GTT GGC CCT GTG ACG CA - #T GAA ATC TTT GAG AAC2592Lys Ala Asp Asp Ile Val Gly Pro Val Thr Hi - #s Glu Ile Phe Glu Asn# 835- AAC GTC GTC CAC TTG ATG TGG CAG GAG CCG AA - #G GAG CCC AAT GGT CTG2640Asn Val Val His Leu Met Trp Gln Glu Pro Ly - #s Glu Pro Asn Gly Leu# 850- ATC GTG CTG TAT GAA GTG AGT TAT CGG CGA TA - #T GGT GAT GAG GAG CTG2688Ile Val Leu Tyr Glu Val Ser Tyr Arg Arg Ty - #r Gly Asp Glu Glu Leu# 865- CAT CTC TGC GTC TCC CGC AAG CAC TTC GCT CT - #G GAA CGG GGC TGC AGG2736His Leu Cys Val Ser Arg Lys His Phe Ala Le - #u Glu Arg Gly Cys Arg870 8 - #75 8 - #80 8 -#85- CTG CGT GGG CTG TCA CCG GGG AAC TAC AGC GT - #G CGA ATC CGG GCC ACC2784Leu Arg Gly Leu Ser Pro Gly Asn Tyr Ser Va - #l Arg Ile Arg Ala Thr# 900- TCC CTT GCG GGC AAC GGC TCT TGG ACG GAA CC - #C ACC TAT TTC TAC GTG2832Ser Leu Ala Gly Asn Gly Ser Trp Thr Glu Pr - #o Thr Tyr Phe Tyr Val# 915- ACA GAC TAT TTA GAC GTC CCG TCA AAT ATT GC - #A AAA ATT ATC ATC GGC2880Thr Asp Tyr Leu Asp Val Pro Ser Asn Ile Al - #a Lys Ile Ile Ile Gly# 930- CCC CTC ATC TTT GTC TTT CTC TTC AGT GTT GT - #G ATT GGA AGT ATT TAT2928Pro Leu Ile Phe Val Phe Leu Phe Ser Val Va - #l Ile Gly Ser Ile Tyr# 945- CTA TTC CTG AGA AAG AGG CAG CCA GAT GGG CC - #G CTG GGA CCG CTT TAC2976Leu Phe Leu Arg Lys Arg Gln Pro Asp Gly Pr - #o Leu Gly Pro Leu Tyr950 9 - #55 9 - #60 9 -#65- GCT TCT TCA AAC CCT GAG TAT CTC AGT GCC AG - #T GAT GTG TTT CCA TGC3024Ala Ser Ser Asn Pro Glu Tyr Leu Ser Ala Se - #r Asp Val Phe Pro Cys# 980- TCT GTG TAC GTG CCG GAC GAG TGG GAG GTG TC - #T CGA GAG AAG ATC ACC3072Ser Val Tyr Val Pro Asp Glu Trp Glu Val Se - #r Arg Glu Lys Ile Thr# 995- CTC CTT CGA GAG CTG GGG CAG GGC TCC TTC GG - #C ATG GTG TAT GAG GGC3120Leu Leu Arg Glu Leu Gly Gln Gly Ser Phe Gl - #y Met Val Tyr Glu Gly# 10105- AAT GCC AGG GAC ATC ATC AAG GGT GAG GCA GA - #G ACC CGC GTG GCG GTG3168Asn Ala Arg Asp Ile Ile Lys Gly Glu Ala Gl - #u Thr Arg Val Ala Val# 10250- AAG ACG GTC AAC GAG TCA GCC AGT CTC CGA GA - #G CGG ATT GAG TTC CTC3216Lys Thr Val Asn Glu Ser Ala Ser Leu Arg Gl - #u Arg Ile Glu Phe Leu# 10451035 - # 1040- AAT GAG GCC TCG GTC ATG AAG GGC TTC ACC TG - #C CAT CAC GTG GTG CGC3264Asn Glu Ala Ser Val Met Lys Gly Phe Thr Cy - #s His His Val Val Arg# 10605- CTC CTG GGA GTG GTG TCC AAG GGC CAG CCC AC - #G CTG GTG GTG ATG GAG3312Leu Leu Gly Val Val Ser Lys Gly Gln Pro Th - #r Leu Val Val Met Glu# 10750- CTG ATG GCT CAC GGA GAC CTG AAG AGC TAC CT - #C CGT TCT CTG CGG CCA3360Leu Met Ala His Gly Asp Leu Lys Ser Tyr Le - #u Arg Ser Leu Arg Pro# 10905- GAG GCT GAG AAT AAT CCT GGC CGC CCT CCC CC - #T ACC CTT CAA GAG ATG3408Glu Ala Glu Asn Asn Pro Gly Arg Pro Pro Pr - #o Thr Leu Gln Glu Met# 11050- ATT CAG ATG GCG GCA GAG ATT GCT GAC GGG AT - #G GCC TAC CTG AAC GCC3456Ile Gln Met Ala Ala Glu Ile Ala Asp Gly Me - #t Ala Tyr Leu Asn Ala# 11251115 - # 1120- AAG AAG TTT GTG CAT CGG GAC CTG GCA GCG AG - #A AAC TGC ATG GTC GCC3504Lys Lys Phe Val His Arg Asp Leu Ala Ala Ar - #g Asn Cys Met Val Ala# 11405- CAT GAT TTT ACT GTC AAA ATT GGA GAC TTT GG - #A ATG ACC AGA GAC ATC3552His Asp Phe Thr Val Lys Ile Gly Asp Phe Gl - #y Met Thr Arg Asp Ile# 11550- TAT GAA ACG GAT TAC TAC CGG AAA GGG GGC AA - #G GGT CTG CTC CCT GTA3600Tyr Glu Thr Asp Tyr Tyr Arg Lys Gly Gly Ly - #s Gly Leu Leu Pro Val# 11705- CGG TGG ATG GCA CCG GAG TCC CTG AAG GAT GG - #G GTC TTC ACC ACT TCT3648Arg Trp Met Ala Pro Glu Ser Leu Lys Asp Gl - #y Val Phe Thr Thr Ser# 11850- TCT GAC ATG TGG TCC TTT GGC GTG GTC CTT TG - #G GAA ATC ACC AGC TTG3696Ser Asp Met Trp Ser Phe Gly Val Val Leu Tr - #p Glu Ile Thr Ser Leu# 12051195 - # 1200- GCA GAA CAG CCT TAC CAA GGC CTG TCT AAT GA - #A CAG GTG TTG AAA TTT3744Ala Glu Gln Pro Tyr Gln Gly Leu Ser Asn Gl - #u Gln Val Leu Lys Phe# 12205- GTC ATG GAT GGA GGG TAT CTG GAT CAA CCC GA - #C AAC TGT CCA GAG AGA3792Val Met Asp Gly Gly Tyr Leu Asp Gln Pro As - #p Asn Cys Pro Glu Arg# 12350- GTC ACT GAC CTC ATG CGC ATG TGC TGG CAA TT - #C AAC CCC AAG ATG AGG3840Val Thr Asp Leu Met Arg Met Cys Trp Gln Ph - #e Asn Pro Lys Met Arg# 12505- CCA ACC TTC CTG GAG ATT GTC AAC CTG CTC AA - #G GAC GAC CTG CAC CCC3888Pro Thr Phe Leu Glu Ile Val Asn Leu Leu Ly - #s Asp Asp Leu His Pro# 12650- AGC TTT CCA GAG GTG TCG TTC TTC CAC AGC GA - #G GAG AAC AAG GCT CCC3936Ser Phe Pro Glu Val Ser Phe Phe His Ser Gl - #u Glu Asn Lys Ala Pro# 12851275 - # 1280- GAG AGT GAG GAG CTG GAG ATG GAG TTT GAG GA - #C ATG GAG AAT GTG CCC3984Glu Ser Glu Glu Leu Glu Met Glu Phe Glu As - #p Met Glu Asn Val Pro# 13005- CTG GAC CGT TCC TCG CAC TGT CAG AGG GAG GA - #G GCG GGG GGC CGG GAT4032Leu Asp Arg Ser Ser His Cys Gln Arg Glu Gl - #u Ala Gly Gly Arg Asp# 13150- GGA GGG TCC TCG CTG GGT TTC AAG CGG AGC TA - #C GAG GAA CAC ATC CCT4080Gly Gly Ser Ser Leu Gly Phe Lys Arg Ser Ty - #r Glu Glu His Ile Pro# 13305- TAC ACA CAC ATG AAC GGA GGC AAG AAA AAC GG - #G CGG ATT CTG ACC TTG4128Tyr Thr His Met Asn Gly Gly Lys Lys Asn Gl - #y Arg Ile Leu Thr Leu# 13450# 4149 TCC TAAPro Arg Ser Asn Pro Ser1350 1355- (2) INFORMATION FOR SEQ ID NO:2:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 1382 amino (B) TYPE: amino acid (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: protein- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:- Met Gly Thr Gly Gly Arg Arg Gly Ala Ala Al - #a Ala Pro Leu Leu Val15- Ala Val Ala Ala Leu Leu Leu Gly Ala Ala Gl - #y His Leu Tyr Pro Gly# 5 1- Glu Val Cys Pro Gly Met Asp Ile Arg Asn As - #n Leu Thr Arg Leu His# 20- Glu Leu Glu Asn Cys Ser Val Ile Glu Gly Hi - #s Leu Gln Ile Leu Leu# 35- Met Phe Lys Thr Arg Pro Glu Asp Phe Arg As - #p Leu Ser Phe Pro Lys# 50- Leu Ile Met Ile Thr Asp Tyr Leu Leu Leu Ph - #e Arg Val Tyr Gly Leu# 65- Glu Ser Leu Lys Asp Leu Phe Pro Asn Leu Th - #r Val Ile Arg Gly Ser# 85- Arg Leu Phe Phe Asn Tyr Ala Leu Val Ile Ph - #e Glu Met Val His Leu# 100- Lys Glu Leu Gly Leu Tyr Asn Leu Met Asn Il - #e Thr Arg Gly Ser Val# 115- Arg Ile Glu Lys Asn Asn Glu Leu Cys Tyr Le - #u Ala Thr Ile Asp Trp# 130- Ser Arg Ile Leu Asp Ser Val Glu Asp Asn Hi - #s Ile Val Leu Asn Lys# 145- Asp Asp Asn Glu Glu Cys Gly Asp Ile Cys Pr - #o Gly Thr Ala Lys Gly150 1 - #55 1 - #60 1 -#65- Lys Thr Asn Cys Pro Ala Thr Val Ile Asn Gl - #y Gln Phe Val Glu Arg# 180- Cys Trp Thr His Ser His Cys Gln Lys Val Cy - #s Pro Thr Ile Cys Lys# 195- Ser His Gly Cys Thr Ala Glu Gly Leu Cys Cy - #s His Ser Glu Cys Leu# 210- Gly Asn Cys Ser Gln Pro Asp Asp Pro Thr Ly - #s Cys Val Ala Cys Arg# 225- Asn Phe Tyr Leu Asp Gly Arg Cys Val Glu Th - #r Cys Pro Pro Pro Tyr230 2 - #35 2 - #40 2 -#45- Tyr His Phe Gln Asp Trp Arg Cys Val Asn Ph - #e Ser Phe Cys Gln Asp# 260- Leu His His Lys Cys Lys Asn Ser Arg Arg Gl - #n Gly Cys His Gln Tyr# 275- Val Ile His Asn Asn Lys Cys Ile Pro Glu Cy - #s Pro Ser Gly Tyr Thr# 290- Met Asn Ser Ser Asn Leu Leu Cys Thr Pro Cy - #s Leu Gly Pro Cys Pro# 305- Lys Val Cys His Leu Leu Glu Gly Glu Lys Th - #r Ile Asp Ser Val Thr310 3 - #15 3 - #20 3 -#25- Ser Ala Gln Glu Leu Arg Gly Cys Thr Val Il - #e Asn Gly Ser Leu Ile# 340- Ile Asn Ile Arg Gly Gly Asn Asn Leu Ala Al - #a Glu Leu Glu Ala Asn# 355- Leu Gly Leu Ile Glu Glu Ile Ser Gly Tyr Le - #u Lys Ile Arg Arg Ser# 370- Tyr Ala Leu Val Ser Leu Ser Phe Phe Arg Ly - #s Leu Arg Leu Ile Arg# 385- Gly Glu Thr Leu Glu Ile Gly Asn Tyr Ser Ph - #e Tyr Ala Leu Asp Asn390 3 - #95 4 - #00 4 -#05- Gln Asn Leu Arg Gln Leu Trp Asp Trp Ser Ly - #s His Asn Leu Thr Thr# 420- Thr Gln Gly Lys Leu Phe Phe His Tyr Asn Pr - #o Lys Leu Cys Leu Ser# 435- Glu Ile His Lys Met Glu Glu Val Ser Gly Th - #r Lys Gly Arg Gln Glu# 450- Arg Asn Asp Ile Ala Leu Lys Thr Asn Gly As - #p Lys Ala Ser Cys Glu# 465- Asn Glu Leu Leu Lys Phe Ser Tyr Ile Arg Th - #r Ser Phe Asp Lys Ile470 4 - #75 4 - #80 4 -#85- Leu Leu Arg Trp Glu Pro Tyr Trp Pro Pro As - #p Phe Arg Asp Leu Leu# 500- Gly Phe Met Leu Phe Tyr Lys Glu Ala Pro Ty - #r Gln Asn Val Thr Glu# 515- Phe Asp Gly Gln Asp Ala Cys Gly Ser Asn Se - #r Trp Thr Val Val Asp# 530- Ile Asp Pro Pro Leu Arg Ser Asn Asp Pro Ly - #s Ser Gln Asn His Pro# 545- Gly Trp Leu Met Arg Gly Leu Lys Pro Trp Th - #r Gln Tyr Ala Ile Phe550 5 - #55 5 - #60 5 -#65- Val Lys Thr Leu Val Thr Phe Ser Asp Glu Ar - #g Arg Thr Tyr Gly Ala# 580- Lys Ser Asp Ile Ile Tyr Val Gln Thr Asp Al - #a Thr Asn Pro Ser Val# 595- Pro Leu Asp Pro Ile Ser Val Ser Asn Ser Se - #r Ser Gln Ile Ile Leu# 610- Lys Trp Lys Pro Pro Ser Asp Pro Asn Gly As - #n Ile Thr His Tyr Leu# 625- Val Phe Trp Glu Arg Gln Ala Glu Asp Ser Gl - #u Leu Phe Glu Leu Asp630 6 - #35 6 - #40 6 -#45- Tyr Cys Leu Lys Gly Leu Lys Leu Pro Ser Ar - #g Thr Trp Ser Pro Pro# 660- Phe Glu Ser Glu Asp Ser Gln Lys His Asn Gl - #n Ser Glu Tyr Glu Asp# 675- Ser Ala Gly Glu Cys Cys Ser Cys Pro Lys Th - #r Asp Ser Gln Ile Leu# 690- Lys Glu Leu Glu Glu Ser Ser Phe Arg Lys Th - #r Phe Glu Asp Tyr Leu# 705- His Asn Val Val Phe Val Pro Arg Lys Thr Se - #r Ser Gly Thr Gly Ala710 7 - #15 7 - #20 7 -#25- Glu Asp Pro Arg Pro Ser Arg Lys Arg Arg Se - #r Leu Gly Asp Val Gly# 740- Asn Val Thr Val Ala Val Pro Thr Val Ala Al - #a Phe Pro Asn Thr Ser# 755- Ser Thr Ser Val Pro Thr Ser Pro Glu Glu Hi - #s Arg Pro Phe Glu Lys# 770- Val Val Asn Lys Glu Ser Leu Val Ile Ser Gl - #y Leu Arg His Phe Thr# 785- Gly Tyr Arg Ile Glu Leu Gln Ala Cys Asn Gl - #n Asp Thr Pro Glu Glu790 7 - #95 8 - #00 8 -#05- Arg Cys Ser Val Ala Ala Tyr Val Ser Ala Ar - #g Thr Met Pro Glu Ala# 820- Lys Ala Asp Asp Ile Val Gly Pro Val Thr Hi - #s Glu Ile Phe Glu Asn# 835- Asn Val Val His Leu Met Trp Gln Glu Pro Ly - #s Glu Pro Asn Gly Leu# 850- Ile Val Leu Tyr Glu Val Ser Tyr Arg Arg Ty - #r Gly Asp Glu Glu Leu# 865- His Leu Cys Val Ser Arg Lys His Phe Ala Le - #u Glu Arg Gly Cys Arg870 8 - #75 8 - #80 8 -#85- Leu Arg Gly Leu Ser Pro Gly Asn Tyr Ser Va - #l Arg Ile Arg Ala Thr# 900- Ser Leu Ala Gly Asn Gly Ser Trp Thr Glu Pr - #o Thr Tyr Phe Tyr Val# 915- Thr Asp Tyr Leu Asp Val Pro Ser Asn Ile Al - #a Lys Ile Ile Ile Gly# 930- Pro Leu Ile Phe Val Phe Leu Phe Ser Val Va - #l Ile Gly Ser Ile Tyr# 945- Leu Phe Leu Arg Lys Arg Gln Pro Asp Gly Pr - #o Leu Gly Pro Leu Tyr950 9 - #55 9 - #60 9 -#65- Ala Ser Ser Asn Pro Glu Tyr Leu Ser Ala Se - #r Asp Val Phe Pro Cys# 980- Ser Val Tyr Val Pro Asp Glu Trp Glu Val Se - #r Arg Glu Lys Ile Thr# 995- Leu Leu Arg Glu Leu Gly Gln Gly Ser Phe Gl - #y Met Val Tyr Glu Gly# 10105- Asn Ala Arg Asp Ile Ile Lys Gly Glu Ala Gl - #u Thr Arg Val Ala Val# 10250- Lys Thr Val Asn Glu Ser Ala Ser Leu Arg Gl - #u Arg Ile Glu Phe Leu# 10451035 - # 1040- Asn Glu Ala Ser Val Met Lys Gly Phe Thr Cy - #s His His Val Val Arg# 10605- Leu Leu Gly Val Val Ser Lys Gly Gln Pro Th - #r Leu Val Val Met Glu# 10750- Leu Met Ala His Gly Asp Leu Lys Ser Tyr Le - #u Arg Ser Leu Arg Pro# 10905- Glu Ala Glu Asn Asn Pro Gly Arg Pro Pro Pr - #o Thr Leu Gln Glu Met# 11050- Ile Gln Met Ala Ala Glu Ile Ala Asp Gly Me - #t Ala Tyr Leu Asn Ala# 11251115 - # 1120- Lys Lys Phe Val His Arg Asp Leu Ala Ala Ar - #g Asn Cys Met Val Ala# 11405- His Asp Phe Thr Val Lys Ile Gly Asp Phe Gl - #y Met Thr Arg Asp Ile# 11550- Tyr Glu Thr Asp Tyr Tyr Arg Lys Gly Gly Ly - #s Gly Leu Leu Pro Val# 11705- Arg Trp Met Ala Pro Glu Ser Leu Lys Asp Gl - #y Val Phe Thr Thr Ser# 11850- Ser Asp Met Trp Ser Phe Gly Val Val Leu Tr - #p Glu Ile Thr Ser Leu# 12051195 - # 1200- Ala Glu Gln Pro Tyr Gln Gly Leu Ser Asn Gl - #u Gln Val Leu Lys Phe# 12205- Val Met Asp Gly Gly Tyr Leu Asp Gln Pro As - #p Asn Cys Pro Glu Arg# 12350- Val Thr Asp Leu Met Arg Met Cys Trp Gln Ph - #e Asn Pro Lys Met Arg# 12505- Pro Thr Phe Leu Glu Ile Val Asn Leu Leu Ly - #s Asp Asp Leu His Pro# 12650- Ser Phe Pro Glu Val Ser Phe Phe His Ser Gl - #u Glu Asn Lys Ala Pro# 12851275 - # 1280- Glu Ser Glu Glu Leu Glu Met Glu Phe Glu As - #p Met Glu Asn Val Pro# 13005- Leu Asp Arg Ser Ser His Cys Gln Arg Glu Gl - #u Ala Gly Gly Arg Asp# 13150- Gly Gly Ser Ser Leu Gly Phe Lys Arg Ser Ty - #r Glu Glu His Ile Pro# 13305- Tyr Thr His Met Asn Gly Gly Lys Lys Asn Gl - #y Arg Ile Leu Thr Leu# 13450- Pro Arg Ser Asn Pro Ser1350 1355- (2) INFORMATION FOR SEQ ID NO:3:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 25 base (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid#= "Primer"A) DESCRIPTION: /desc- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:# 25 ATGT GCTGG- (2) INFORMATION FOR SEQ ID NO:4:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 24 base (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid#= "Primer"A) DESCRIPTION: /desc- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:# 24GTCA GAAT- (2) INFORMATION FOR SEQ ID NO:5:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 19 base (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid#= "Probe"(A) DESCRIPTION: /desc- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:# 19 TGT__________________________________________________________________________

Number	Name	Date	Kind
5260200	Kahn et al.	Nov 1993
5621075	Kahn et al.	Apr 1997

Mutant human insulin receptor DNA

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Krook et al., "Rapid and simultanoues detection of multiple mutations by pooled . . ." Hum. Molec. Gene. 1(6):391-395 (1992).
Makino et al., Mini Review Insulin Receptor Gene Mutation: A Molecular Genetical and Functional Analysis Cell. Sign. 4(4):351-363 (1992).
Kan et al., "Frequency of Mutations of Insulin Receptor Gene in Japanese Patients with NIDDM" Diabetes 44:1081-1086 (1995).