Claims
- 1. An isolated nucleic acid molecule selected from the group consisting of:
a) a nucleic acid molecule comprising a nucleotide sequence which is at least about 60% identical to the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:12, or the cDNA insert of the plasmid deposited with ATCC as Accession Number ______ ______, ______ or ______ a complement thereof; b) a nucleic acid molecule comprising a fragment of at least 439 nucleotides of the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, or a complement thereof; c) a nucleic acid molecule comprising a fragment of at least 481 nucleotides of the nucleotide sequence of SEQ ID NO:4, SEQ ID NO:6, the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, or a complement thereof; d) a nucleic acid molecule comprising a fragment of at least 2175 nucleotides of the nucleotide sequence of SEQ ID NO:7, SEQ ID NO:9, the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, or a complement thereof; e) a nucleic acid molecule comprising a fragment of at least 439 (CHECK NUMBER) nucleotides of the nucleotide sequence of SEQ ID NO:10, SEQ ID NO:12, the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, or a complement thereof; f) a nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence of at least about 60% homologous to the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:11, or an amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, ______ or ______; g) a nucleic acid molecule which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:5 or SEQ ID NO:8, or SEQ ID NO:11, wherein the fragment comprises at least 15 contiguous amino acids of SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:11, or the polypeptide encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, ______ or ______; and h) a nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:11, or an amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, ______ or ______, wherein the nucleic acid molecule hybridizes to a nucleic acid molecule comprising SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:12, or a complement thereof under stringent conditions.
- 2. The isolated nucleic acid molecule of claim 1, which is selected from the group consisting of:
a) a nucleic acid comprising the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:12, the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, ______ or ______, or a complement thereof; and b) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:11, or an amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number Number ______, ______ or ______.
- 3. The nucleic acid molecule of claim 1 further comprising vector nucleic acid sequences.
- 4. The nucleic acid molecule of claim 1 further comprising nucleic acid sequences encoding a heterologous polypeptide.
- 5. A host cell which contains the nucleic acid molecule of claim 1.
- 6. The host cell of claim 5 which is a mammalian host cell.
- 7. A non-human mammalian host cell containing the nucleic acid molecule of claim 1.
- 8. An isolated polypeptide selected from the group consisting of:
a) a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:11, or the polypeptide encoded by the DNA insert of the plasmid deposited with ATCC as Accession Number ______, ______ or ______, wherein the fragment comprises at least 15 contiguous amino acids of SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:11, or the amino acid sequence encoded by the DNA insert of the plasmid deposited with ATCC as Accession Number ______, ______ or ______; b) a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:5; SEQ ID NO:8, SEQ ID NO:11, or an amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, ______ or ______, wherein the polypeptide is encoded by a nucleic acid molecule which hybridizes to a nucleic acid molecule comprising SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:12 or a complement thereof under stringent conditions; and c) a polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 60% identical to a nucleic acid comprising the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:12, or a complement thereof.
- 9. The isolated polypeptide of claim 8 comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:11, or an amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number ______ or ______.
- 10. The polypeptide of claim 8 further comprising heterologous amino acid sequences.
- 11. An antibody which selectively binds to a polypeptide of claim 8.
- 12. A method for producing a polypeptide selected from the group consisting of:
a) a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:11, or an amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number ______ or ______; b) a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:11, or an amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number Number ______, ______ or ______, wherein the fragment comprises at least 15 contiguous amino acids of SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:11, or an amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number ______ ______ or ______; and c) a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:11, or an amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, ______ or ______, wherein the polypeptide is encoded by a nucleic acid molecule which hybridizes to a nucleic acid molecule comprising SEQ ID NO:1, SEQ ID NO:3, SEQ D NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:12, or a complement thereof under stringent conditions; comprising culturing the host cell of claim 5 under conditions in which the nucleic acid molecule is expressed.
- 13. A method for detecting the presence of a polypeptide of claim 8 in a sample, comprising:
a) contacting the sample with a compound which selectively binds to a polypeptide of claim 8; and b) determining whether the compound binds to the polypeptide in the sample.
- 14. The method of claim 13, wherein the compound which binds to the polypeptide is an antibody.
- 15. A kit comprising a compound which selectively binds to a polypeptide of claim 8 and instructions for use.
- 16. A method for detecting the presence of a nucleic acid molecule of claim 1 in a sample, comprising the steps of:
a) contacting the sample with a nucleic acid probe or primer which selectively hybridizes to the nucleic acid molecule; and b) determining whether the nucleic acid probe or primer binds to a nucleic acid molecule in the sample.
- 17. The method of claim 16, wherein the sample comprises mRNA molecules and is contacted with a nucleic acid probe.
- 18. A kit comprising a compound which selectively hybridizes to a nucleic acid molecule of claim 1 and instructions for use.
- 19. A method for identifying a compound which binds to a polypeptide of claim 8 comprising:
a) contacting a polypeptide, or a cell expressing a polypeptide of claim 8 with a test compound; and b) determining whether the polypeptide binds to the test compound.
- 20. The method of claim 19, wherein the binding of the test compound to the polypeptide is detected by a method selected from the group consisting of:
a) detection of binding by direct detecting of test compound/polypeptide binding; b) detection of binding using a competition binding assay; c) detection of binding using an assay for LGR6-activity.
- 21. A method for modulating the activity of a polypeptide of claim 8 comprising contacting a polypeptide or a cell expressing a polypeptide of claim 8 with a compound which binds to the polypeptide in a sufficient concentration to modulate the activity of the polypeptide.
- 22. A method for identifying a compound which modulates the activity of a polypeptide of claim 8, comprising:
a) contacting a polypeptide of claim 8 with a test compound; and b) determining the effect of the test compound on the activity of the polypeptide to thereby identify a compound which modulates the activity of the polypeptide.
RELATED APPLICATIONS
[0001] This application claims priority to U.S. patent application Ser. No. 09/566,588, filed May 8, 2000, which claims the benefit U.S. Provisional Application No.: 60/132,896 filed on May 6, 1999, incorporated herein in their entirety by this reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60132896 |
May 1999 |
US |
Continuations (1)
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Number |
Date |
Country |
Parent |
09851595 |
May 2001 |
US |
Child |
10664667 |
Sep 2003 |
US |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
09566588 |
May 2000 |
US |
Child |
09851595 |
May 2001 |
US |