Claims
- 1. An isolated nucleic acid molecule selected from the group consisting of:
a) a nucleic acid molecule comprising a nucleotide sequence which is at least 80% identical to the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:4; b) a nucleic acid molecule comprising a fragment of at least 500 nucleotides of the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:4; c) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2; d) a nucleic acid molecule which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, wherein the fragment comprises at least 100 contiguous amino acids of SEQ ID NO:2; and e) a nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, wherein the nucleic acid molecule hybridizes to a nucleic acid molecule comprising SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:4, or a complement thereof, under stringent conditions.
- 2. The isolated nucleic acid molecule of claim 1, further comprising a fragment of at least 600 nucleotides of the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:4.
- 3. The isolated nucleic acid molecule of claim 1, further comprising a fragment of at least 700 nucleotides of the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:4.
- 4. The isolated nucleic acid molecule of claim 1, further comprising a fragment of at least 950 nucleotides of the nucleotide sequence of SEQ ID NO:1 or SEQ ID NO:3.
- 5. The isolated nucleic acid molecule of claim 1, which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, wherein the fragment comprises at least 400 contiguous amino acids of SEQ ID NO:2.
- 6. The isolated nucleic acid molecule of claim 1, which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:5, wherein the fragment comprises at least 500 contiguous amino acids of SEQ ID NO:2.
- 7. The isolated nucleic acid molecule of claim 1, which is selected from the group consisting of:
a. a nucleic acid comprising the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:4; and b. a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2.
- 8. The nucleic acid molecule of claim 1 further comprising vector nucleic acid sequences.
- 9. The nucleic acid molecule of claim 1 further comprising nucleic acid sequences encoding a heterologous polypeptide.
- 10. A host cell which contains the nucleic acid molecule of claim 1.
- 11. The host cell of claim 10 which is a mammalian host cell.
- 12. A non-human mammalian host cell containing the nucleic acid molecule of claim 1.
- 13. An isolated polypeptide selected from the group consisting of:
a) a polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 80% identical to a nucleic acid comprising the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, or a complement thereof; b) a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, wherein the polypeptide is encoded by a nucleic acid molecule which hybridizes to a nucleic acid molecule comprising SEQ ID NO:1 or SEQ ID NO:3; and c) a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, wherein the fragment comprises at least 100 contiguous amino acids of SEQ ID NO:2.
- 14. The isolated polypeptide of claim 13, comprising a fragment which comprises at least 200 contiguous amino acids of SEQ ID NO:2.
- 15. The isolated polypeptide of claim 13, comprising a fragment which comprises at least 500 contiguous amino acids of SEQ ID NO:2.
- 16. The isolated polypeptide of claim 13, comprising a fragment which comprises at least 700 contiguous amino acids of SEQ ID NO:2.
- 17. The isolated polypeptide of claim 13, comprising a fragment which is at least 90% homologous to the amino acid sequence of SEQ ID NO:2.
- 18. The isolated polypeptide of claim 13, comprising a fragment which is at least 95% homologous to the amino acid sequence of SEQ ID NO:2.
- 19. The isolated polypeptide of claim 13, comprising the amino acid sequence of SEQ ID NO:2.
- 20. The polypeptide of claim 13 further comprising heterologous amino acid sequences.
- 21. An antibody which selectively binds to a polypeptide of claim 13.
- 22. The antibody of claim 21, which is a monoclonal antibody.
- 23. The antibody of claim 22, comprising an immunologically active portion selected from the group consisting of:
a) an scFV fragment; b) a dcFV fragment; c) an Fab fragment; and d) an F(ab′)2 fragment.
- 24. The antibody of claim 22, wherein the antibody is selected from the group consisting of:
a) a chimeric antibody; b) a humanized antibody; c) a human antibody; d) a non-human antibody; and e) a single chain antibody.
- 25. A method for producing a polypeptide selected from the group consisting of:
a) a polypeptide comprising the amino acid sequence of SEQ ID NO:2; b) a polypeptide comprising a fragment of the amino acid sequence of SEQ ID NO:2, wherein the fragment comprises at least 100 contiguous amino acids of SEQ ID NO:2; c) a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, or the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, wherein the polypeptide is encoded by a nucleic acid molecule which hybridizes to a nucleic acid molecule comprising SEQ ID NO:1, SEQ ID NO:3, or a complement thereof under stringent conditions; comprising culturing the host cell of claim 10 under conditions in which the nucleic acid molecule is expressed.
- 26. A method for detecting the presence of a polypeptide of claim 13 in a sample, comprising:
contacting the sample with a compound which selectively binds to a polypeptide of claim 13; and determining whether the compound binds to the polypeptide in the sample.
- 27. The method of claim 26, wherein the compound which binds to the polypeptide is an antibody.
- 28. A kit comprising a compound which selectively binds to a polypeptide of claim 13 and instructions for use.
- 29. A method for detecting the presence of a nucleic acid molecule of claim 1 in a sample, comprising the steps of:
contacting the sample with a nucleic acid probe or primer which selectively hybridizes to the nucleic acid molecule; and determining whether the nucleic acid probe or primer binds to a nucleic acid molecule in the sample.
- 30. The method of claim 29, wherein the sample comprises mRNA molecules and is contacted with a nucleic acid probe.
- 31. A kit comprising a compound which selectively hybridizes to a nucleic acid molecule of claim 1 and instructions for use.
- 32. A method for identifying a compound which binds to a polypeptide of claim 13 comprising the steps of:
contacting a polypeptide, or a cell expressing a polypeptide of claim 13 with a test compound; and determining whether the polypeptide binds to the test compound.
- 33. The method of claim 32, wherein the binding of the test compound to the polypeptide is detected by a method selected from the group consisting of:
a) detection of binding by direct detecting of test compound/polypeptide binding; b) detection of binding using a competition binding assay; and c) detection of binding using an assay for 52908-mediated signal transduction.
- 34. A method for modulating the activity of a polypeptide of claim 13 comprising contacting a polypeptide or a cell expressing a polypeptide of claim 13 with a compound which binds to the polypeptide in a sufficient concentration to modulate the activity of the polypeptide.
- 35. A method for identifying a compound which modulates the activity of a polypeptide of claim 13, comprising:
contacting a polypeptide of claim 13 with a test compound; and determining the effect of the test compound on the activity of the polypeptide to thereby identify a compound which modulates the activity of the polypeptide.
RELATED APPLICATIONS
[0001] This application claims priority to U.S. application Ser. No. 09/586,340 filed on Jun. 20, 2000 which claims priority to U.S. Provisional Application No. 60/137,659 filed on Jun. 4, 1999, incorporated herein in its entirety by this reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60137659 |
Jun 1999 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09586340 |
Jun 2000 |
US |
Child |
10461747 |
Jun 2003 |
US |