Claims
- 1. A process for producing a saccharide-transferred product, comprising the steps of:
- introducing a recombinant DNA carrying isolated promoter and structural gene sequences coding for the expression of a polypeptide possessing cyclomaltodextrin glucanotransferase (CGTase) activity and comprising one or more partial amino acid sequences selected from the group consisting of:
- (a) Asn-Lys-Ile-Asn-Asp-Gly-Tyr-Leu-Thr,
- (b) Pro-Val-Phe-Thr-Phe-Gly-Glu-Trp-Phe-Leu,
- (c) Val-Thr-Phe-Ile-Asp-Asn-His-Asp-Met-Asp-Arg-Phe,
- (d) Ile-Tyr-Tyr-Gly-Thr-Glu-Gln-Tyr-Met-Thr-Gly-Asn-Gly-Asp-Pro-Asn-Asn-Arg, and
- (e) Asn-Pro-Ala-Leu-Ala-Tyr-Gly, into a host microorganism to obtain a recombinant microorganism;
- culturing with a nutrient culture medium said recombinant microorganism having said recombinant DNA for the expression of said polypeptide;
- recovering the accumulated polypeptide; and
- subjecting an amylaceous substance to the action of said recovered polypeptide possessing cyclomaltodextrin glucanotransferase activity to produce a saccharide-transferred product.
- 2. The process in accordance with claim 1, wherein said saccharide-transferred product is cyclodextrin.
- 3. The process in accordance with claim 1, wherein said amylaceous substance is subjected to the action of said polypeptide in the presence of a saccharide acceptor.
- 4. The process in accordance with claim 1, wherein said amylaceous substance is selected from the group consisting of starch, amylose, cyclodextrin, dextrin, and mixtures thereof.
- 5. The process in accordance with claim 3, wherein said saccharide acceptor is a member selected from the group consisting of saccharide sweetener, glycoside, and mixtures thereof.
- 6. The process in accordance with claim 3, wherein the saccharide-transferred product is a member selected from the group consisting of .alpha.-glycosytsucrose, .alpha.-glycosyl stevioside, and .alpha.-glycosyl ginsenoside.
- 7. The process in accordance with claim 3, wherein said saccharide-transferred product is used as a sweetener.
- 8. The process in accordance with claim 1, wherein said host microorganism is of the genus Escherichia or Bacillus.
- 9. The process in accordance with claim 1, wherein said recombinant microorganism is a member selected from the group consisting of Escherichia coli TCH201 (FERM BP-2109) or Escherichia coli MAH2 (FERM BP-2110).
- 10. The process in accordance with claim 1, wherein said recombinant microorganism is a member selected from the group consisting of Bacillus subtilis MAU210 (FERM BP-2111) and Bacillus subtilis TCU211 (FERM BP-2112).
- 11. The process in accordance with claim 1, wherein said polypeptide possessing cyclomaltodextrin glucanotransferase activity has the following amino acid sequence:
- __________________________________________________________________________1 2 3 4 5 6 7 8 9 10 11 12 13 14 15__________________________________________________________________________ 1> Ala Gly Asn Leu Asn Lys Val Asn Phe Thr Ser Asp Val Val Tyr 16> Gln Ile Val Val Asp Arg Phe Val Asp Gly Asn Thr Ser Asn Asn 31> Pro Ser Gly Ala Leu Phe Ser Ser Gly Cys Thr Asn Leu Arg Lys 46> Tyr Cys Gly Gly Asp Trp Gln Gly Ile Ile Asn Lys Ile Asn Asp 61> Gly Tyr Leu Thr Asp Met Gly Val Thr Ala Ile Trp Ile Ser Gln 76> Pro Val Glu Asn Val Phe Ser Val Met Asn Asp Ala Ser Gly Ser 91> Ala Ser Tyr His Gly Tyr Trp Ala Arg Asp Phe Lys Lys Pro Asn106> Pro Phe Phe Gly Thr Leu Ser Asp Phe Gln Arg Leu Val Asp Ala121> Ala His Ala Lys Gly Ile Lys Val Ile Ile Asp Phe Ala Pro Asn136> His Thr Ser Pro Ala Ser Glu Thr Asn Pro Ser Tyr Met Glu Asn151> Gly Arg Leu Tyr Asp Asn Gly Thr Leu Leu Gly Gly Tyr Thr Asn166> Asp Ala Asn Met Tyr Phe His His Asn Gly Gly Thr Thr Phe Ser181> Ser Leu Glu Asp Gly Ile Tyr Arg Asn Leu Phe Asp Leu Ala Asp196> Leu Asn His Gln Asn Pro Val Ile Asp Arg Tyr Leu Lys Asp Ala211> Val Lys Met Trp Ile Asp Met Gly Ile Asp Gly Ile Arg Met Asp226> Ala Val Lys His Met Pro Phe Gly Trp Gln Lys Ser Leu Met Asp241> Glu Ile Asp Asn Tyr Arg Pro Val Phe Thr Phe Gly Glu Trp Phe256> Leu Ser Glu Asn Glu Val Asp Ala Asn Asn His Tyr Phe Ala Asn271> Glu Ser Gly Met Ser Leu Leu Asp Phe Arg Phe Gly Gln Lys Leu286> Arg Gln Val Leu Arg Asn Asn Ser Asp Asn Trp Tyr Gly Phe Asn301> Gln Met Ile Gln Asp Thr Ala Ser Ala Tyr Asp Glu Val Leu Asp316> Gln Val Thr Phe Ile Asp Asn His Asp Met Asp Arg Phe Met Ile331> Asp Gly Gly Asp Pro Arg Lys Val Asp Met Ala Leu Ala Val Leu346> Leu Thr Ser Arg Gly Val Pro Asn Ile Tyr Tyr Gly Thr Glu Gln361> Tyr Met Thr Gly Asn Gly Asp Pro Asn Asn Arg Lys Met Met Ser376> Ser Phe Asn Lys Asn Thr Arg Ala Tyr Gln Val Ile Gln Lys Leu391> Ser Ser Leu Arg Arg Asn Asn Pro Ala Leu Ala Tyr Gly Asp Thr406> Glu Gln Arg Trp Ile Asn Gly Asp Val Tyr Val Tyr Glu Arg Gln421> Phe Gly Lys Asp Val Val Leu Val Arg Val Asn Arg Ser Ser Ser436> Ser Asn Tyr Ser Ile Thr Gly Leu Phe Thr Ala Leu Pro Ala Gly451> Thr Tyr Thr Asp Gln Leu Gly Gly Leu Leu Asp Gly Asn Thr Ile466> Gln Val Gly Ser Asn Gly Ser Val Asn Ala Phe Asp Leu Gly Pro481> Gly Glu Val Gly Val Trp Ala Tyr Ser Ala Thr Glu Ser Thr Pro496> Ile Ile Gly His Val Gly Pro Met Met Gly Gln Val Gly His Gln511> Val Thr Ile Asp Gly Glu Gly Phe Gly Thr Asn Thr Gly Thr Val526> Lys Phe Gly Thr Thr Ala Ala Asn Val Val Ser Trp Ser Asn Asn541> Gln Ile Val Val Ala Val Pro Asn Val Ser Pro Gly Lys Tyr Asn556> Ile Thr Val Gln Ser Ser Ser Gly Gln Thr Ser Ala Ala Tyr Asp571> Asn Phe Glu Val Leu Thr Asn Asp Gln Val Ser Val Arg Phe Val586> Val Asn Asn Ala Thr Thr Asn Leu Gly Gln Asn Ile Tyr Ile Val601> Gly Asn Val Tyr Glu Leu Gly Asn Trp Asp Thr Ser Lys Ala Ile616> Gly Pro Met Phe Asn Gln Val Val Tyr Ser Tyr Pro Thr Trp Tyr631> Ile Asp Val Ser Val Pro Glu Gly Lys Thr Ile Glu Phe Lys Phe646> Ile Lys Lys Asp Ser Gln Gly Asn Val Thr Trp Glu Ser Gly Ser661> Asn His Val Tyr Thr Thr Pro Thr Asn Thr Thr Gly Lys Ile Ile676> Val Asp Trp Gln Asn__________________________________________________________________________
- 12. The process in accordance with claim 1, wherein said polypeptide possessing cyclomaltodextrin glucanotransferase activity has the following amino acid sequence:
- __________________________________________________________________________1 2 3 4 5 6 7 8 9 10 11 12 13 14 15__________________________________________________________________________ 1> Ser Pro Asp Thr Ser Val Asn Asn Lys Leu Asn Phe Ser Thr Asp 16> Thr Val Tyr Gln Ile Val Thr Asp Arg Phe Val Asp Gly Asn Ser 31> Ala Asn Asn Pro Thr Gly Ala Ala Phe Ser Ser Asp His Ser Asn 46> Leu Lys Leu Tyr Phe Gly Gly Asp Trp Gln Gly Ile Thr Asn Lys 61> Ile Asn Asp Gly Tyr Leu Thr Gly Met Gly Ile Thr Ala Leu Trp 76> Ile Ser Gln Pro Val Glu Asn Ile Thr Ala Val Ile Asn Tyr Ser 91> Gly Val Asn Asn Thr Ala Tyr His Gly Tyr Trp Pro Arg Asp Phe106> Lys Lys Thr Asn Ala Ala Phe Gly Ser Phe Thr Asp Phe Ser Asn121> Leu Ile Ala Ala Ala His Ser His Asn Ile Lys Val Val Met Asp136> Phe Ala Pro Asn His Thr Asn Pro Ala Ser Ser Thr Asp Pro Ser151> Phe Ala Glu Asn Gly Ala Leu Tyr Asn Asn Gly Thr Leu Leu Gly166> Lys Tyr Ser Asn Asp Thr Ala Gly Leu Phe His His Asn Gly Gly181> Thr Asp Phe Ser Thr Thr Glu Ser Gly Ile Tyr Lys Asn Leu Tyr196> Asp Leu Ala Asp Ile Asn Gln Asn Asn Asn Thr Ile Asp Ser Tyr211> Leu Lys Glu Ser Ile Gln Leu Trp Leu Asn Leu Gly Val Asp Gly226> Ile Arg Phe Asp Ala Val Lys His Met Pro Gln Gly Trp Gln Lys241> Ser Tyr Val Ser Ser Ile Tyr Ser Ser Ala Asn Pro Val Phe Thr256> Phe Gly Glu Trp Phe Leu Gly Pro Asp Glu Met Thr Gln Asp Asn271> Ile Asn Phe Ala Asn Gln Ser Gly Met His Leu Leu Asp Phe Ala286> Phe Ala Gln Glu Ile Arg Glu Val Phe Arg Asp Lys Ser Glu Thr301> Met Thr Asp Leu Asn Ser Val Ile Ser Ser Thr Gly Ser Ser Tyr316> Asn Tyr Ile Asn Asn Met Val Thr Phe Ile Asp Asn His Asp Met331> Asp Arg Phe Gln Gln Ala Gly Ala Ser Thr Arg Pro Thr Glu Gln346> Ala Leu Ala Val Thr Leu Thr Ser Arg Gly Val Pro Ala Ile Tyr361> Tyr Gly Thr Glu Gln Tyr Met Thr Gly Asn Gly Asp Pro Asn Asn376> Arg Gly Met Met Thr Gly Phe Asp Thr Asn Lys Thr Ala Tyr Lys391> Val Ile Lys Ala Leu Ala Pro Leu Arg Lys Ser Asn Pro Ala Leu406> Ala Tyr Gly Ser Thr Thr Gln Arg Trp Val Asn Ser Asp Val Tyr421> Val Tyr Glu Arg Lys Phe Gly Ser Asn Val Ala Leu Val Ala Val436> Asn Arg Ser Ser Thr Thr Ala Tyr Pro Ile Ser Gly Ala Leu Thr451> Ala Leu Pro Asn Gly Thr Tyr Thr Asp Val Leu Gly Gly Leu Leu466> Asn Gly Asn Ser Ile Thr Val Asn Gly Gly Thr Val Ser Asn Phe481> Thr Leu Ala Ala Gly Gly Thr Ala Val Trp Gln Tyr Thr Thr Thr496> Glu Ser Ser Pro Ile Ile Gly Asn Val Gly Pro Thr Met Gly Lys511> Pro Gly Asn Thr Ile Thr Ile Asp Gly Arg Gly Phe Gly Thr Thr526> Lys Asn Lys Val Thr Phe Gly Thr Thr Ala Val Thr Gly Ala Asn541> Ile Val Ser Trp Glu Asp Thr Glu Ile Lys Val Lys Val Pro Asn556> Val Ala Ala Gly Asn Thr Ala Val Thr Val Thr Asn Ala Ala Gly571> Thr Thr Ser Ala Ala Phe Asn Asn Phe Asn Val Leu Thr Ala Asp586> Gln Val Thr Val Arg Phe Lys Val Asn Asn Ala Thr Thr Ala Leu601> Gly Gln Asn Val Tyr Leu Thr Gly Asn Val Ala Glu Leu Gly Asn616> Trp Thr Ala Ala Asn Ala Tle Gly Pro Met Tyr Asn Gln Val Glu631> Ala Ser Tyr Pro Thr Trp Tyr Phe Asp Val Ser Val Pro Ala Asn646> Thr Ala Leu Gln Phe Lys Phe Ile Lys Val Asn Gly Ser Thr Val661> Thr Trp Glu Gly Gly Asn Asn His Thr Phe Thr Ser Pro Ser Ser676> Gly Val Ala Thr Val Thr Val Asp Trp Gln Asn__________________________________________________________________________
- 13. The process in accordance with claim 1, wherein said recombinant DNA comprises the following DNA sequence:
- __________________________________________________________________________10 20 30 40 50 60GCTGGAAATC TTAA TAAGGT AAACT TTACA TCAGATG TTG TCTATCAAAT TGTAGTGGAT70 80 90 100 110 120CGAT TTGTGG ATGGAAATAC ATCCAA TAAT CCGAGTGGAG CATTATTTAG CTCAGGATGT130 140 150 160 170 180ACGA ATTTAC GCAAGTATTG CGGTGGAGAT TGGCAAGGCA TA TCAAT AA AAT TAACGAT190 200 210 220 230 240GGGTAT TTAA CAGATATGGG TGTGACAGCG ATAT GGATTT CTCAGCCTGT AGAAAATGTA250 260 270 280 290 300TT TTCTGTGA TGAATGATGC AAGCGGTTCC GCATCCTATC ATGG TTATTG GGCGCGCGAT310 320 330 340 350 360TTCAAAAAGC CAAACCCGTT TT TTGGTACC CTCAGTGAT T TCCAACGTTT AGTTGATGCC370 380 390 400 410 420GCACATGCAA AAGGAATAAA GGTAATTATT GAC TTTGCCC CCAACCATAC TTCTCCTGCT430 440 450 460 470 480TCAGAAACGA AT CCT TCTTA TATGGAAAAC GGACGACTGT ACGATAATGG GACATTGCTT490 500 510 520 530 540GGCGGTTACA CAAATGATGC CAACATGTAT T TTCACCA TA ACGGTGGAAC AACGTTTTCC550 560 570 580 590 600AGCTT AGAGG ATGGGATTTA TCGAAATCTG TTTGACT TGG CGGACCTTAA CCATCAGAAC610 620 630 640 650 660CCTGT TA TTG ATAGG TATTT AAAAGATGCA GTAAAAA TGT GGA TAGATAT GGGGATTGAT670 680 690 700 710 720GGTAT CCGTA TGGATGCGGT GAAGCACATG CCGTTTG GAT GGCAAAAATC TCTGATGGAT730 740 750 760 770 780GAGAT TGATA AC TATCGTCC TGTCTTTACG TT TGGGGAGT GG TTTTTGTC AGAAAATGAA790 800 810 820 830 840GTGGACGCGA ACAATCAT TA CTTTGCCAAT GAAAGTGG AA TGAGT TTGCT CGAT TTTCGT850 860 870 880 890 900TTCGGACAAA AGCTTCGTCA AGTATTGCGC AATAACAGCG ATAAT TGGTA TGGC TTTAAT910 920 930 940 950 960CAAATGATTC AAGATACGGC ATCAGCATAT GACGAGGTTC TCG ATCAAGT AACAT TCATA970 980 990 1000 1010 1020GACAACCATG AT ATGGATCG GTT TATGATT GACGGAGGAG ATCCGCGCAA GGTGGATATG1030 1040 1050 1060 1070 1080GCACTTGCTG TA TTATTGAC ATCCCGTGGC GTACCGAA TA T TTACTATGG TACAGAGCAA1090 1100 1110 1120 1130 1140TACATGACCG GTAACGGCGA TCCAAACAAT CGTAAGATGA TGAGTTCATT CAA TAAAAAT1150 1160 1170 1180 1190 1200ACTCGCGCGT ATCAAGT GAT TCAAAAACTA TCT TCTCTCC GACGAAACAA TCCGGCGTTA1210 1220 1230 1240 1250 1260GCT TATGGTG ATACGGAACA GCGTTGGATC AATGGCGATG TG TATGTGT A TGAGCGACAG1270 1280 1290 1300 1310 1320T TTGGCAAAG ATG TTGTGTT AGTT CGGGTT AATCGT AGTT CAAGCAGTAA TTAC TCGATT1330 1340 1350 1360 1370 1380ACTGGC TTAT TTACAGCTTT ACCAGCAGGA ACATATACGG ATCAGCT TGG CGGTC TTTTA1390 1400 1410 1420 1430 1440GACGGAAATA CAA TTCAAGT CGGTTCAAAT GGATCAGT TA ATGCATT TGA CTTAGGACCG1450 1460 1470 1480 1490 1500GGGGAAGTCG GTGTATGGGC ATACAGTGCA ACAGAAAGCA CGCCAATTAT TGGTCATGT T1510 1520 1530 1540 1550 1560GGACCGATGA TGGGGCAAGT CGGTCATCAA GTAACCATTG ATGGCGAAGG ATTCGGAACA1570 1580 1590 1600 1610 1620AATACGGGCA CTGTGAAGTT CGGAACGACA GCTGCCAATG TTGTG TCT TG GTCTAACAAT1630 1640 1650 1660 1670 1680CAAATCGTTG TGGCTGTACC AAATGTG TCA CCAGGAAAAT ATAATATTAC CGTCCAATCA1690 1700 1710 1720 1730 1740TCAAGCGGTC AAACGAGTGC GGCTTATGAT AAC TTTGAAG TACTAACAAA TGATCAAGTG1750 1760 1770 1780 1790 1800TCAGTGCGGT TTGT TGTTAA TAACGCGACT ACCAATCTAG GGCAAAA TAT ATACATTG TT1810 1820 1830 1840 1850 1860GGCAACGTAT ATGAGCTCGG CAACTGGGAC ACTAGTAAGG CAATCGGTCC AATGT TCAAT1870 1880 1890 1900 1910 1920CAAGTGGT TT ACTCCTATCC TACATGGTA T ATAGATGTCA GTGTCCCAGA AGGAAAGACA1930 1940 1950 1960 1970 1980ATT GAGTT TA AGT TTATTAA AAAAGACAGC CAAGGTAATG TCACTTGGGA AAGTGGTTCA1990 2000 2010 2020 2030 2040AATCATG T TT ATACGACACC AACGAATACA ACCGGAAAAA TTATAGTGGA TTGGCAGAAC__________________________________________________________________________
- 14. The process in accordance with claim 1, wherein said recombinant DNA comprises the following DNA sequence:
- __________________________________________________________________________10 20 30 40 50 60TCCCCGGATA CGAGCGTGAA CAACAAGCTC AAT TTTAGCA CGGA TACGGT T TACCAGATT70 80 90 100 110 120GTAACCGACC GGTTTGTGGA CGGCAAT TCC GCCAACAACC CGACCGGAGC AGCCTTCAGC130 140 150 160 170 180AGCGATCA TT CCAACCTGAA GCTGTATTTC GGGGGCGACT GGCAGGGGAT CACGAACAAA190 200 210 220 230 240ATCAACGACG GCTATCTGAC CGGAATGGGC ATCACCGCCC TCTGGA TCTC GCAGCCGGTT250 260 270 280 290 300GAGAACATCA CCGCCGTCAT CAATTA TTCG GGCGTCAACA ATACAG CTTA CC ACGGTTAC310 320 330 340 350 360TGGCCTCGCG ACTTCAAGAA GACCAATGCC GCGTTCGGCA GCTTCACCGA CT TC TCCAAT370 380 390 400 410 420TTGATCGCCG CAGCGCATTC ACACAATATC AAGGTAGT TA TGGACTT TGC ACCT AATCAC430 440 450 460 470 480ACCAACCCGG CTTCGAGTAC GGACCCCTCG TTCGCCGAGA ACGGCGCGCT CTACAACAAC490 500 510 520 530 540GGAACGCTGC TCGGCAAGTA TAGCAACGAT ACCGCCGGCC TG TTCCACCA CAATGGCGGC550 560 570 580 590 600ACCGAT TTC T CGACGACTGA AAGCGGTATC TACAAGAACC TGTACGATCT CGCGGATATC610 620 630 640 650 660AATCAGA ACA ACAACACCAT CGACTCGTAT CTCAAGGAAT CGATCCAGCT GTGGCTGAAT670 680 690 700 710 720CTCGGAGTCG ACGGCATCCG CTTCGACGCC GTGAAGCATA TGCCTCAGGG CTGGCAGAAG730 740 750 760 770 780AGCTACG TCT CGTCGATCTA CAGCAGCGCC AATCCGGTGT TCACC TTCGG TGAATGGTTC790 800 810 820 830 840CTCGGCCCCG ACGAAATGAC CCAGGACAAC ATCAACTTCG CGAATCAGAG CGGCATGCAC850 860 870 880 890 900CTGCTG GACT TTGCGTTTGC GCAGGAAATC CGTGAAGTGT TCCGCGACAA GTCGGAGACG910 920 930 940 950 960ATGACCGACC TGAACTCGGT GATCTCCAGC ACCGGCTCCA GCTATAA TTA CATCAACAAC970 980 990 1000 1010 1020ATGG TTACGT TCATCGACAA CCATGACATG GACCGCTTCC AGCAAGCCGG AGCGAGCACT1030 1040 1050 1060 1070 1080CGCCCGACCG AGCAGGCTCT TGCGGTAACG CTGACTTCCC GCGGCGTTCC GGCAATCTAC1090 1100 1110 1120 1130 1140TACGGTACAG AGCAA TATAT GACCGGCAAC GGCGACCCGA ACAACCGCGG CATGATGACC1150 1160 1170 1180 1190 1200GGCTTCGATA CGAACAAGAC AGCGTACAAA GT GATCAAGG CGCTGGCTCC GCTTCGCAAG1210 1220 1230 1240 1250 1260TCCAACCCGG CTCTCGCCTA CGGCTCGACG AC CCAGCGTT GGGTGAACAG CGACGTCTAC1270 1280 1290 1300 1310 1320GTAT ATGAAC GCAAGTTCGG AAGCAACGTA GC T TTCGTTG CCGTCAACCG CAGCTCGACG1330 1340 1350 1360 1370 1380ACTGCCT ATC CGATATCGGG AGCGC TTACT GC TCTGCCAA ACGGAACGTA TACCGACGTT1390 1400 1410 1420 1430 1440CTCGGCGGCC TGC TTAATGG CAATT CAATT AC CGTTAACG GCGGCACGGT CAGCAA CTTT1450 1460 1470 1480 1490 1500ACACT TGCAG CGGGCGGTAC GGCAGTCTGG CAGTACACGA CGACGGAATC CTCGCC GATT1510 1520 1530 1540 1550 1560ATCGGCAACG TCGGCCCGAC TATGGGCAAG CCCGGCAACA CCATCACGAT CGACGGACGC1570 1580 1590 1600 1610 1620GGCTT CGGTA CTACGAAGAA CAAAGTT ACT T TCGGTACGA CAGCCGTTAC CGGCGCGAAC1630 1640 1650 1660 1670 1680ATCGTGAGCT GGGAAGATAC CGAAATCAAG GTCAAAG TTC CGAACGTGGC CGCCGGCAAC1690 1700 1710 1720 1730 1740ACGGCCGTTA CGGTAACGAA CGCCGCCGGC ACTACCAGCG CAGCGTTCAA CAACT T TAAC1750 1760 1770 1780 1790 1800GTACTGACTG CCGA TCAGGT CACTGTCCGC TTCAAAGTCA ACAATGCCAC CACGGCCCTG1810 1820 1830 1840 1850 1860GGACAAAACG TCTACCTGAC CGGTAACGTC GCCGAGCTTG GCAACTGGAC AGCCGCCAAC1870 1880 1890 1900 1910 1920GCAATCGGTC CGATGTACAA CCAGGTAGAA GCCAGCTATC CGAC TTGGTA CT TCGACGTC1930 1940 1950 1960 1970 1980AGCGTTCCGG CCAACACGGC GCTGCAATTC AA GTTCATCA AAGTGAACGG CT CGACAGTG1990 2000 2010 2020 2030 2040ACTTGGGAAG GCGGCAACAA CCACACCTTC ACCTCGCCTT CGAGCGGCGT TGCGACCGTA2050 2060ACGGTCGATT GGCAGAAC__________________________________________________________________________
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a division of application Ser. No. 07/794,347, filed Nov. 12, 1991, now U.S. Pat. No. 5,278,059 which is a continuation of application Ser. No. 07/438,993, filed Nov. 22, 1989, now abandoned which was a continuation of application Ser. No. 06,804,487, filed Dec. 4, 1985, now abandoned.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
3988206 |
Shiosake |
Oct 1976 |
|
4219571 |
Miyake |
Aug 1980 |
|
Divisions (1)
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Number |
Date |
Country |
Parent |
794347 |
Nov 1991 |
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Continuations (2)
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Number |
Date |
Country |
Parent |
438993 |
Nov 1989 |
|
Parent |
804487 |
Dec 1985 |
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