Claims
- 1. A plasmid encoding a recombinant IBDV VP-2 polypeptide, which is capable of inducing antibodies that passively protect chickens against infection by IBDV, comprising the native amino acid sequence of IBDV VP-2, wherein the five N-terminal amino acid residues of the native VP-2 sequence, MTLNS, have been replaced by the octapeptide MFSELDPQ.
- 2. A plasmid of claim 1 selected from the group consisting of pYELC5.PO, pYELC5.VP2T and pYELC5.PO.DELTA.Xho1.
- 3. Plasmid pYELC5.PO.
- 4. Plasmid pYELC.5.VP2T.
- 5. Plasmid pYELC5.PO.DELTA.Xho1.
- 6. Plasmid pK.lactis.VP2T.
- 7. A host cell comprising a plasmid encoding a recombinant IBDV VP-2 polypeptide, which is capable of inducing antibodies that passively protect chickens against infection by IBDV, comprising the native amino acid sequence of IBDV VP-2, wherein the five N-terminal amino acid residues of the native VP-2 sequence, MTLNS, have been replaced by the octapeptide MFSELDPQ.
- 8. A Saccharomyces cerevisiae host cell comprising a plasmid selected from the group consisting of pYELC5.PO, pYELC5.VP2T and pYELC5.PO.DELTA.Xho1.
- 9. A Saccharomyces cerevisiae host cell comprising plasmid pYELC5.PO.
- 10. A Saccharomyces cerevisiae host cell comprising plasmid pYELC.5.VP2T.
- 11. A Saccharomyces cerevisiae host cell comprising plasmid pYELC5.PO.DELTA.Xho1.
- 12. A Klulyveromyces lactis host cell comprising plasmid pK.lactis.VP2T.
- 13. A recombinant IBDV VP-2 polypeptide, which is capable of inducing antibodies that passively protect chickens against infection by IBDV, comprising the native amino acid sequence of IBDV VP-2, wherein the five N-terminal amino acid residues of the native VP-2 sequence, MTLNS, have been replaced by an octapeptide selected from the group consisting of MFSELDPQ and MNSSSVPG.
- 14. A recombinant IBDV VP-2 polypeptide of claim 13 expressed in Saccharomyces cerevisiae by a plasmid selected from the group consisting of pYELC5.PO, pYELC5.VP2T and pYELC5.PO.DELTA.Xho1.
- 15. A recombinant IBDV VP-2 polypeptide expressed in Saccharomyces cerevisiae by plasmid pYELC5.PO.
- 16. A recombinant IBDV VP-2 polypeptide expressed in Saccharomyces cerevisiae by plasmid pYELC.5.VP2T.
- 17. A recombinant IBDV VP-2 polypeptide expressed in Saccharomyces cerevisiae by plasmid pYELC5.PO.DELTA.Xho1.
- 18. A recombinant IBDV VP-2 polypeptide expressed in Kluveromyces lactis by plasmid pK.lactis.VP2T.
Priority Claims (1)
Number |
Date |
Country |
Kind |
PJ4469 |
May 1989 |
AUX |
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Parent Case Info
This application is a continuation of application Ser. No. 07/776,411, filed Nov. 21, 1991, now abandoned.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
4511652 |
Fogel et al. |
Apr 1985 |
|
4940661 |
Etcheverry et al. |
Jul 1990 |
|
5350575 |
Azad et al. |
Sep 1994 |
|
Foreign Referenced Citations (3)
Number |
Date |
Country |
8815845 |
Nov 1988 |
AUX |
276730 |
Aug 1988 |
EPX |
8502545 |
Jun 1985 |
WOX |
Continuations (1)
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Number |
Date |
Country |
Parent |
776411 |
Nov 1991 |
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