Claims
- 1. A complex between two polypeptides of adipocytes as defined in columns 1 and 4 in Table 2.
- 2. A complex between two polynucleotides of adipocytes, said polynucleotides encoding two polypeptides as defined in columns 1 and 4 in Table 2.
- 3. A recombinant host cell expressing a polynucleotide encoding an adipocyte polypeptide as defined in columns 1 and 4 in Table 2.
- 4. A method for selecting a modulating compound that inhibits or activates interactions between two polypeptides of adipocytes comprising:
cultivating a recombinant host cell containing a reporter gene the expression of which is toxic for said recombinant host cell, on a selective medium comprising a modulating compound, wherein said recombinant host cell is transformed with two vectors:
wherein said first vector comprises a polynucleotide encoding a first hybrid polypeptide and a DNA binding domain; wherein said second vector comprises a polynucleotide encoding a second hybrid polypeptide and an activating domain that activates said reporter gene when the first and second hybrid polypeptides interact; and selecting said modulating compound which inhibits the growth of said recombinant host cell.
- 5. A modulating compound obtained from the method of claim 4.
- 6. A SID polypeptide comprising SEQ ID NOS: 28, 30 or 32, or odd sequences starting from SEQ ID NOS: 49 to 1173, inclusive, in column 4 of Table 3, or a fragment or a variant thereof.
- 7. A variant of the SID polypeptide of claim 6.
- 8. A fragment of the SID polypeptide of claim 6.
- 9. A SID polynucleotide comprising SEQ ID NOS: 27, 29 or 31, or even sequences starting from SEQ ID NOS: 48 to 1172, inclusive, in column 2 of Table 3, or a fragment or a variant thereof.
- 10. A fragment of the SID polynucleotide of claim 9.
- 11. A variant of the SID polynucleotide of claim 9.
- 12. A vector comprising the SID polynucleotide of claim 9.
- 13. A vector comprising the fragment of the SID polynucleotide of claim 10.
- 14. A recombinant host cell comprising the vector of claim 12.
- 15. A pharmaceutical composition comprising a modulating compound that inhibits or activates interactions between polypeptides of adipocytes, and a pharmaceutically acceptable carrier.
- 16. A pharmaceutical composition comprising a SID polypeptide designated by SEQ ID NOS: 28, 30 or 32, or odd sequences starting from SEQ ID NOS: 49 to 1173, inclusive, in column 4 of Table 3, and a pharmaceutically acceptable carrier.
- 17. A pharmaceutical composition comprising the recombinant host cell of claim 14 and a pharmaceutically acceptable carrier.
- 18. A protein chip comprising a polypeptide of adipocytes as defined in columns 1 and 2 of Table 1.
- 19. A record comprising all or part of the data set forth in Tables 1 and 2.
PRIORITY
[0001] This application claims priority on the basis of U.S. Provisional Application No. 60/288,885 filed May 4, 2001, incorporated herein by reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60288885 |
May 2001 |
US |