Claims
- 1. A fusion protein comprising green fluorescent protein (GFP) and a PEST Sequence, said protein having a half life of no more than about ten hours.
- 2. The fusion protein of claim 1, wherein said GFP is enhanced green fluorescent protein (EGFP).
- 3. The fusion protein of claim 2, wherein said fusion protein comprises EGFP fused to a PEST sequence from murine ornithine decarboxylase (MODC).
- 4. The fusion protein of claim 3, wherein said PEST sequence is selected from the group consisting of MODC fragments MODC.sub.376-461, MODC.sub.376-456, and MODC.sub.422-461, and variants of MODC.sub.376-461, MODC.sub.376-456, and MODC.sub.422-461, containing amino acid substitutions selected from the group consisting of P26A/P427A, P438A, E428A/E430A/E431A, E444A, S440A, S445A, T436A, D433A/D434A and D448A.
- 5. The EGFP fusion protein of claim 3, wherein said protein has the sequence shown in SEQ ID No. 1.
CROSS-REFERENCE TO RELATED APPLICATION
This application claims benefit of priority to provisional application U.S. Ser. No. 60/060,855, filed Oct. 2, 1997.