Claims
- 1. Biomolecular conjugates comprising the conjugation product of a repeat sequence protein polymer and at least one active agent.
- 2. The biomolecular conjugates as recited in claim 1, wherein the repeat sequence protein polymer comprises a repeating amino acid sequence unit derived from elastin, collagen, abductin, byssus, flagelliform silk, dragline silk, gluten high molecular weight subunit, titin, fibronectin, leminin, gliadin, glue polypolypeptide, ice nucleating protein, keratin, mucin, RNA polymerase II, resalin or a mixture thereof.
- 3. The biomolecular conjugates as recited in claim 1, wherein the repeat sequence protein polymer formula comprises:
- 4. The biomolecular conjugates as recited in claim 3, wherein T and T′ comprise an amino acid sequence from about 1 to about 60 amino acids.
- 5. The biomolecular conjugates as recited in claim 3, wherein T and T′ comprise an amino acid sequence with fewer than 20% of the total number of amino acids in the repeat sequence protein polymer.
- 6. The biomolecular conjugates as recited in claim 3, wherein B comprises an amino acid sequence with a biological or chemical function or activity.
- 7. The biomolecular conjugates as recited in claim 3, wherein B′ comprises an amino acid sequence with a biological or chemical function or activity.
- 8. The biomolecular conjugates as recited in claim 3, wherein i is an integer from 1 to 50.
- 9. The biomolecular conjugates as recited in claim 3, wherein i is an integer from 1 to about 30.
- 10. The biomolecular conjugates as recited in claim 3, wherein A, A′, and A″ comprise SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 20 or a combination thereof.
- 11. The biomolecular conjugates as recited in claim 3, wherein A, A′, and A″ comprise SEQ ID NO. 1, SEQ ID NO. 3 or a combination thereof.
- 12. The biomolecular conjugates as recited in claim 1, wherein the repeat sequence protein polymer comprises SEQ ID NO. 19.
- 13. The biomolecular conjugates as recited in claim 1, wherein the at least one active agent is selected from the group consisting of: silicones, fragrances, dyes, tints, UV actives, sunscreens, lanolin, vitamins, bleaches, thickening agents, proteins, peptides, enzymes, antimicrobials, preservatives, and combinations thereof.
- 14. The biomolecular conjugates as recited in claim 13, wherein the at least one active agent comprises a silicone.
- 15. The biomolecular conjugates as recited in claim 14, wherein the silicone is selected from the group consisting of: silicones comprising an epoxy functional group, polydimethyl siloxanes, silicone resins, silicone surfactants, alkyl modified silicones, gum-silocone fluid blends, phenyl silicone fluids, dimethyl silicone fluids, quaternized ammonium salts of suitable silicones, silicones comprising one or more amino functional groups, and combinations thereof.
- 16. A personal care composition comprising the biomolecular conjugates as recited in claim 1.
- 17. The personal care composition as recited in claim 16 comprising a hair care composition, a skin care composition, a nail care composition, a cosmetic composition, an oral care composition, or an over-the-counter pharmaceutical composition.
- 18. The personal care composition as recited in claim 16 wherein the biomolecular conjugates comprise from about 0.001% to about 10% by weight of the composition
- 19. The personal care composition as recited in claim 16 wherein the biomolecular conjugates comprise from about 0.01% to about 5% by weight of the composition.
- 20. The personal care composition as recited in claim 16 wherein the biomolecular conjugates comprise from about 0.01% to about 1% by weight of the composition
- 21. A process for making a personal care composition comprising combining a biomolecular conjugate with an acceptable carrier or excipient to obtain a personal care composition, wherein the biomolecular conjugate comprises a conjugation product of a repeat sequence protein polymer and at least one active agent.
- 22. The biomolecular conjugates as recited in claim 1 formulated into an emulsion comprising, by weight of the emulsion composition:
- 23. A personal care product composition comprising the emulsion as recited in claim 22.
- 24. The biomolecular conjugates as recited in claim 1 formulated into a surfactant system comprising, by weight of the surfactant system composition:
- 25. A personal care product composition comprising the surfactant system as recited in claim 24.
- 26. The biomolecular conjugate as recited in claim 1 wherein the repeat sequence protein polymer comprises a silk elastin polymer and the at least one active agent comprises a protein or peptide, and further wherein the conjugation product comprises a fusion protein.
- 27. The biomolecular conjugate as recited in claim 26 wherein the silk elastin polymer comprises SELP47K (SEQ. ID. NO. 19), and the protein or peptide comprises any protein or peptide suitable for a desired application and which is capable of forming a conjugation product comprising a fusion protein with SELP47K (SEQ. ID. NO. 19).
- 28. The biomolecular conjugate as recited in claim 26, wherein the peptide comprises an antimicrobial peptide.
- 29. The biomolecular conjugate as recited in claim 26 comprising CAM-SELP47K (SEQ. ID. NO. 32).
- 30. The biomolecular conjugate as recited in claim 26 comprising MBI-SELP47K (SEQ. ID. NO. 33)
- 31. The biomolecular conjugate as recited in claim 26, wherein the protein comprises a green fluorescent protein.
- 32. The biomolecular conjugate as recited in claim 26 comprising GFP-SELP47K (SEQ. ID. NO. 34).
- 33. The biomolecular conjugate as recited in claim 26, wherein the peptide comprises a cotton binding peptide.
- 34. The biomolecular conjugate as recited in claim 26 comprising CBP-SELP47K-S2E1 (SEQ. ID. NO. 35).
- 35. The biomolecular conjugate as recited in claim 26, wherein the peptide comprises P4.
- 36 The biomolecular conjugate as recited in claim 26 comprising P4-SELP47K (SEQ. ID. NO. 36).
- 37. The biomolecular conjugate as recited in claim 26 comprising at least two active agents, and wherein the at least two active agents are different.
- 38. A method of producing a biomolecular conjugate comprising a fusion protein conjugate comprising a conjugation product of a repeat sequence protein polymer and at least one active agent comprising a protein or peptide, the method comprising: selecting the repeat sequence protein polymer and the protein or peptide active suitable for a desired application; obtaining a gene encoding the repeat sequence protein polymer and a gene encoding the at least one active agent comprising a protein or peptide; constructing a conjugate gene from the gene encoding the repeat sequence protein polymer and the gene encoding the at least one active agent comprising a protein or peptide; expressing the conjugate gene to form an expression product comprising the fusion protein conjugate; fermenting the expression product comprising the fusion protein conjugate; and purifying the fusion protein conjugate.
- 39. The method as recited in claim 38 comprising at least two active agents.
- 40. The method as recited in claim 38 wherein the at least one active agent comprises a protein or a peptide comprising an antimicrobial peptide, P4, a cotton binding peptide, green fluorescent protein, or a combination thereof.
- 41. A method of providing biomaterial adapted for at least one predetermined desirable function comprising: selecting a biomolecular conjugate comprising a conjugation product of a repeat sequence protein polymer and at least one active agent, wherein the repeat sequence protein polymer comprises a silk elastin polymer and the at least one active agent comprises a protein or peptide, and further wherein the conjugation product comprises a fusion protein, according to the predetermined desirable function; and incorporating the biomolecular conjugate into a material.
- 42. The method as recited in claim 41, wherein the method provides biomaterial adapted for more than one predetermined desirable function and wherein the biomolecular conjugate comprises at least two active agents according to the predetermined desirable function.
- 43. A biomaterial adapted for at least one predetermined desirable function comprising at least one biomolecular conjugate comprising a conjugation product of a repeat sequence protein polymer and at least one active agent, wherein the repeat sequence protein polymer comprises a silk elastin polymer and the active agent comprises a protein or peptide, and further wherein the conjugation product comprises a fusion protein.
- 44. The biomaterial as recited in claim 43 wherein the at least one biomolecular conjugate comprises AMP-SELP47K, GFP-SELP47K, P4-SELP47K, CBP-SELP47K, or some combination thereof.
- 45. The biomaterial as recited in claim 44, wherein the biomolecular conjugate comprises AMP-SELP47K, and wherein the biomaterial comprises a textile, a filter, a cream or a coating.
- 46. The biomaterial as recited in claim 45, wherein the textile comprises a protective garment.
- 47. The biomaterial as recited in claim 46 wherein the protective garment comprises a surgical gown or a garment designed for biological warfare defense.
- 48. The biomaterial as recited in claim 43, wherein the biomolecular conjugate comprises GFP-SELP47K, and wherein the biomaterial elicits a detectable output in response to a predetermined stimuli.
- 49. The biomaterial as recited in claim 48, wherein the biomaterial comprises a genetics research tool or a search and/or identification tool.
- 50. The biomaterial as recited in claim 43, wherein the biomolecular conjugate comprises P4-SELP47K, wherein the biomaterial comprises a textile, dressing, cream or coating for the protection of ultraviolet radiation sensitive surfaces.
- 51. A repeat sequence protein polymer comprising the biomolecular conjugate as recited in claim 1.
RELATED APPLICATION
[0001] This application claims priority under 35 U.S.C. §119 of U.S. Provisional Application Ser. No. 60/470,464 filed May 14, 2003.
Provisional Applications (1)
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Number |
Date |
Country |
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60470464 |
May 2003 |
US |