Claims
- 1. An isolated DNA coding for a variable (V) region of a heavy (H) chain of an antibody to the human IL-6 receptor having the following structure:
- FR1.sup.2 -CDR1.sup.2 -FR2.sup.2 -CDR2.sup.2 -FR3.sup.2 -CDR3.sup.2 -FR4.sup.2
- wherein CDR1.sup.2, CDR2.sup.2 and CDR3.sup.2 represent a set of three complementarity determining regions comprising a set of the following amino acid sequences shown by:
- ______________________________________SEQ ID No. 125 CDR1.sup.2 Ser Asp His Ala Trp SerSEQ ID No. 126 CDR2.sup.2 Tyr Ile Ser Tyr Ser Gly Ile Thr Thr Tyr Asn Pro Ser Leu Lys SerSEQ ID No. 127 CDR3.sup.2 Ser Leu Ala Arg Thr Thr Ala Met Asp Tyr;______________________________________
- and FR1.sup.2, FR2.sup.2, FR3.sup.2 and FR4.sup.2 comprise a set of the following amino acid sequences:
- ______________________________________SEQ ID No. 135 FR1.sup.2 Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Arg Pro Ser Gln Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Tyr Ser Ile ThrSEQ ID No. 129 FR2.sup.2 Trp Val Arg Gln Pro Pro Gly Arg Gly Leu Glu Trp Ile GlySEQ ID No. 132 FR3.sup.2 Arg Val Thr Met Leu Arg Asp Thr Ser Lys Asn Gln Phe Ser Leu Arg Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala ArgSEQ ID No. 131 FR4.sup.2 Trp Gly Gln Gly Ser Leu Val Thr Val Ser Ser.______________________________________
- 2. An isolated DNA coding for an H chain of an antibody to the human IL- 6 receptor, comprising:
- (1) a V region of a heavy chain of an antibody to the human IL-6 receptor having the following structure:
- FR1.sup.2 -CDR1.sup.2 -FR2.sup.2 -CDR1.sup.2 -FR3.sup.2 -CDR3.sup.2 -FR4.sup.2
- wherein CDR1.sup.2, CDR2.sup.2, and CDR3.sup.2 represent a set of three complementarity determining regions comprising a set of the following amino acid sequences:
- ______________________________________SEQ ID No. 125 CDR1.sup.2 Ser Asp His Ala Trp SerSEQ ID No. 126 CDR2.sup.2 Tyr Ile Ser Tyr Ser Gly Ile Thr Thr Tyr Asn Pro Ser Leu Lys SerSEQ ID No. 127 CDR3.sup.2 Ser Ala Arg Thr Thr Ala Met Asp Tyr;______________________________________
- and FR1.sup.2, FR2.sup.2, FR3.sup.2 and FR4.sup.2 comprise a set of the following amino acid sequences:
- ______________________________________SEQ ID No. 135 FR1.sup.2 Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Arg Pro Ser Gln Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Tyr Ser Ile ThrSEQ ID No. 129 FR2.sup.2 Trp Val Arg Gln Pro Pro Gly Arg Gly Leu Glu Trp Ile GlySEQ ID No. 132 FR3.sup.2 Arg Val Thr Met Leu Arg Asp Thr Ser Lys Asn Gln Phe Ser Leu Arg Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala ArgSEQ ID No. 131 FR4.sup.2 Trp Gly Gln Gly Ser Leu Val Thr Val Ser Ser;______________________________________
- and
- (2) a C region of an H chain of a human antibody C.gamma..
- 3. An isolated DNA coding for a reshaped human antibody to the human IL-6 receptor, comprising
- (A) L chains of an antibody to said human IL-6 receptor, each comprising:
- (1) a variable (V) region of a light (L) chain of an antibody to the human IL-6 receptor having the following structure:
- FR1.sup.1 -CDR1.sup.1 -FR2.sup.1 -CDR2.sup.1 -FR3.sup.3 -CDR3.sup.1 -FR4.sup.1
- wherein CDR1.sup.1, CDR2.sup.1 and CDR3.sup.1 represent a set of three complementarity determining regions comprising a set of the following amino acid sequences:
- ______________________________________SEQ ID No. 117 CDR1.sup.2 Arg Ala Ser Gln Asp Ile Ser Ser Tyr Leu AsnSEQ ID No. 118 CDR2.sup.1 Tyr Thr Ser Arg Leu His SerSEQ ID No. 119 CDR3.sup.1 Gln Gln Gly Asn Thr Leu Pro Tyr Thr;______________________________________
- and the FR1.sup.1, FR2.sup.1, FR3.sup.1 and FR4.sup.1 comprise a set of the following amino acid sequences:
- ______________________________________SEQ ID No. 120 FR1.sup.1 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr CysSEQ ID No. 121 FR2.sup.1 Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile TyrSEQ ID No. 122 FR3.sup.1 Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr Tyr CysSEQ ID No. 123 FR4.sup.1 Phe Gly Gln Gly Thr Lys Val Glu Ile Lys;orSEQ ID No. 120 FR1.sup.1 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr CysSEQ ID No. 121 FR2.sup.1 Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile TyrSEQ ID No. 124 FR3.sup.1 Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr Tyr CysSEQ ID No. 1234 FR.sup.1 Phe Gly Gln Gly Thr Lys Val Glu Ile Lys;______________________________________
- and
- (2) a C region of an L chain of a human antibody C.kappa.; and
- (B) H chains of an antibody to the human IL-6 receptor, each comprising:
- (1) a V region of a heavy (H) chain of an antibody to the human IL-6 receptor having the following structure:
- FR1.sup.2 -CDR1.sup.2 -FR2.sup.2 -CDR2.sup.2 -FR3.sup.2 -CDR3.sup.2 -FR4.sup.2
- wherein CDR1.sup.2, CDR2.sup.2 and CDR3.sup.2 represent a set of three complementarity determining regions comprising a set of the amino acid sequences shown by:
- ______________________________________SEQ ID No. 125 CDR1.sup.2 Ser Asp His Ala Trp SerSEQ ID No. 126 CDR2.sup.2 Tyr Ile Ser Tyr Ser Gly Ile Thr Thr Tyr Asn Pro Ser Leu Lys SerSEQ ID No. 127 CDR3.sup.2 Ser Leu Ala Arg Thr Thr Ala Met Asp Tyr;______________________________________
- and the FR1.sup.2, FR2.sup.2, FR3.sup.2 and FR4.sup.2 comprise a set of the following amino acid sequences:
- ______________________________________SEQ ID No. 135 FR1.sup.2 Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Arg Pro Ser Gln Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Tyr Ser Ile ThrSEQ ID No. 129 FR2.sup.2 Trp Val Arg Gln Pro Pro Gly Arg Gly Leu Glu Trp Ile GlySEQ ID No. 132 FR3.sup.2 Arg Val Thr Met Leu Arg Asp Thr Ser Lys Asn Gln Phe Ser Leu Arg Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr CysAla ArgSEQ ID No. 131 FR4.sup.2 Trp Gly Gln Gly Ser Leu Val Thr Val Ser Ser;______________________________________
- and
- (2) a C region of an H chain of a human antibody C.gamma..
- 4. An isolated DNA coding for a V region of a heavy (H) chain of an antibody to the human IL-6 receptor having the following structure:
- FR1.sup.4 -CDR14-FR2.sup.4 -CDR2.sup.4 -FR3.sup.4 -CDR3.sup.4 -FR4.sup.4
- wherein CDR1.sup.4, CDR2.sup.4 and CDR3.sup.4, represent a set of three complementarity determining regions comprising a set of the following amino acid sequences:
- __________________________________________________________________________SEQ ID No. 143 CDR1.sup.4 Ser Tyr Tyr Ile HisSEQ ID No. 144 CDR2.sup.4 Tyr Ile Asp Pro Phe Asn Gly Gly Thr Ser Tyr Asn Gln Lys Phe Lys GlySEQ ID No. 145 CDR3.sup.4 Gly Gly Asn Arg Phe Ala Tyr;__________________________________________________________________________
- and the FR1.sup.4, FR2.sup.4, FR3.sup.4 and FR4.sup.4 comprise a set of the following amino acid sequences:
- __________________________________________________________________________SEQ ID No. 146 FR1.sup.4 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe ThrSEQ ID No. 147 FR2.sup.4 Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Val GlySEQ ID No. 150 FR3.sup.4 Lys Val Thr Met Thr Val Asp Thr Ser Thr Asn Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala ArgSEQ ID No. 149 FR4.sup.4 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser;SEQ ID No. 146 FR1.sup.4 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe ThrSEQ ID No. 151 FR2.sup.4 Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile GlySEQ ID NO. 150 FR3.sup.4 Lys Val Thr Met Thr Val Asp Thr Ser Thr Asn Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala ArgSEQ ID No. 149 FR4.sup.4 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser;SEQ ID No. 152 FR1.sup.4 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe ThrSEQ ID No. 155 FR2.sup.4 Trp Val Arg Gln Ala Pro Gly Gln Arg Leu Glu Trp Ile GlySEQ ID No. 154 FR3.sup.4 Arg Val Thr Ile Thr Val Asp Thr Ser Ala Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala ArgSEQ ID No. 149 FR4.sup.4 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser;SEQ ID No. 152 FR1.sup.4 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe ThrSEQ ID No. 153 FR2.sup.4 Trp Val Arg Gln Ala Pro Gly Gln Arg Leu Glu Trp Met GlySEQ ID No. 156 FR3.sup.4 Lys Val Thr Ile Thr Val Asp Thr Ser Ala Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala ArgSEQ ID No. 149 FR4.sup.4 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser;orSEQ ID No. 152 FR1.sup.4 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe ThrSEQ ID No. 155 FR2.sup.4 Trp Val Arg Gln Ala Pro Gly Gln Arg Leu Glu Trp Ile GlySEQ ID No. 156 FR3.sup.4 Lys Val Thr Ile Thr Val Asp Thr Ser Ala Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala ArgSEQ ID No. 149 FR4.sup.4 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser.__________________________________________________________________________
- 5. An isolated DNA coding for an H chain of an antibody to the human IL- 6 receptor, comprising:
- (1) a V region of heavy (H) chain of an antibody to the human IL-6 receptor having the following structure:
- FR1.sup.4 -CDR1.sup.4 -FR2.sup.4 -CDR2.sup.4 -FR3.sup.4 -CDR3.sup.4 -FR4.sup.4
- where CDR1.sup.4, CDR2.sup.4, and CDR3.sup.4 represent a set of three complementarity determining regions comprising a set of the following sequences shown by:
- __________________________________________________________________________SEQ ID No. 143 CDR1.sup.4 Ser Tyr Tyr Ile HisSEQ ID No. 144 CDR2.sup.4 Tyr Ile Asp Pro Phe Asn Gly Gly Thr Ser Tyr Asn Gln Lys Phe Lys GlySEQ ID No. 145 CDR3.sup.4 Gly Gly Asn Arg Phe Ala Tyr;__________________________________________________________________________
- and the FR1.sup.4, FR2.sup.4, FR3.sup.4, and FR4.sup.4 comprise a set of the following amino acid sequences:
- __________________________________________________________________________SEQ ID No. 146 FR1.sup.4 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe ThrSEQ ID No. 147 FR2.sup.4 Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Val GlySEQ ID No. 150 FR3.sup.4 Lys Val Thr Met Thr Val Asp Thr Ser Thr Asn Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala ArgSEQ ID No. 149 FR4.sup.4 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser;SEQ ID No. 146 FR1.sup.4 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe ThrSEQ ID No. 151 FR2.sup.4 Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile GlySEQ ID No. 150 FR3.sup.4 Lys Val Thr Met Thr Val Asp Thr Ser Thr Asn Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala ArgSEQ ID No. 149 FR4.sup.4 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser;SEQ ID No. 157 FR1.sup.4 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe ThrSEQ ID No. 158 FR2.sup.4 Trp Val Arg Gln Ala Pro Gly Gln Arg Leu Glu Trp Ile GlySEQ ID No. 154 FR3.sup.4 Arg Val Thr Ile Thr Val Asp Thr Ser Ala Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala ArgSEQ ID No. 149 FR4.sup.4 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser;SEQ ID No. 152 FR1.sup.4 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe ThrSEQ ID No. 153 FR2.sup.4 Trp Val Arg Gln Ala Pro Gly Gln Arg Leu Glu Trp Met GlySEQ ID No. 156 FR3.sup.4 Lys Val Thr Ile Thr Val Asp Thr Ser Ala Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala ArgSEQ ID No. 149 FR4.sup.4 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser;orSEQ ID No. 152 FR1.sup.4 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe ThrSEQ ID No. 155 FR2.sup.4 Trp Val Arg Gln Ala Pro Gly Gln Arg Leu Glu Trp Ile GlySEQ ID No. 156 FR3.sup.4 Lys Val Thr Ile Thr Val Asp Thr Ser Ala Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala ArgSEQ ID No. 149 FR4.sup.4 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser;__________________________________________________________________________
- and
- (2) a C region of an H chain of a human antibody C.gamma..
- 6. An isolated DNA coding for a reshaped human antibody to the human IL-6 receptor, comprising:
- (A) L chains of an antibody to the human IL-6 receptor, each comprising:
- (1) a variable (V) region of a light (L) chain of an antibody to the human IL-6 receptor having the following structure:
- FR1.sup.3 -CDR1.sup.3 -FR2.sup.3 -CDR2.sup.3 -FR3.sup.3 -CDR3.sup.3 -FR4.sup.3
- wherein CDR1.sup.3, CDR2.sup.3 and CDR3.sup.3 represent a set of three complementarity determining regions comprising a set of the following amino acid sequences:
- ______________________________________SEQ ID No. 136 CDR1.sup.3 Arg Ala Ser Lys Ser Val Ser Thr Ser Gly Tyr Ser Tyr Met HisSEQ ID No. 137 CDR2.sup.3 Ala Ser Asn Leu Glu SerSEQ ID No. 138 CDR3.sup.3 Gln His Ser Arg Glu Asn Pro Tyr Thr;______________________________________
- and FR1.sup.3, FR2.sup.3, FR3.sup.3 and FR4.sup.3 comprise a set of the following amino acid sequences:
- ______________________________________SEQ ID No. 139 FR1.sup.3 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr CysSEQ ID No. 140 FR2.sup.3 Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile TyrSEQ ID No. 141 FR3.sup.3 Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr Tyr CysSEQ ID No. 142 FR4.sup.3 Phe Gly Gln Gly Thr Lys Val Glu Ile Lys;______________________________________
- and
- (2) a C region of an L chain of a human antibody C.kappa.;
- and
- (B) H chains of an antibody to the human IL-6 receptor, each comprising:
- (1) a V region of a heavy (H) chain of an antibody to the human IL-6 receptor having the following structure:
- FR1.sup.4 -CDR1.sup.4 -FR2.sup.4 -CDR2.sup.4 -FR3.sup.4 -CDR3.sup.4 -FR4.sup.4
- wherein CDR1.sup.4, CDR2.sup.4 and CDR3.sup.4 represent a set of three complementarity determining regions comprising a set of the following amino acid sequences:
- ______________________________________SEQ ID No. 143 CDR1.sup.4 Ser Tyr Tyr Ile HisSEQ ID No. 144 CDR2.sup.4 Tyr Ile Asp Pro Phe Asn Gly Gly Thr Ser Tyr Asn Gly Lys Phe Lys GlySEQ ID No. 145 CDR3.sup.4 Gly Gly Asn Arg Phe Ala Tyr;______________________________________
- and FR1.sup.4, FR2.sup.4, FR3.sup.4 and FR4.sup.4 comprise a set of the following amino acid sequences:
- ______________________________________SEQ ID No. 146 FR1.sup.4 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe ThrSEQ ID No. 147 FR2.sup.4 Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Val GlySEQ ID No. 150 FR3.sup.4 Lys Val Thr Met Thr Val Asp Thr Ser Thr Asn Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala ArgSEQ ID No. 149 FR4.sup.4 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser;SEQ ID No. 146 FR1.sup.4 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe ThrSEQ ID No. 151 FR2.sup.4 Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile GlySEQ ID No. 150 FR3.sup.4 Lys Val Thr Met Thr Val Asp Thr Ser Thr Asn Thr Asn Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala ArgSEQ ID No. 149 FR4.sup.4 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser;SEQ ID No. 152 FR1.sup.4 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe ThrSEQ ID No. 155 FR2.sup.4 Trp Val Arg Gln Ala Pro Gly Gln Arg Leu Glu Trp Ile GlySEQ ID No. 154 FR3.sup.4 Arg Val Thr Ile Thr Val Asp Thr Ser Ala Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala ArgSEQ ID No. 149 FR4.sup.4 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser;SEQ ID No. 152 FR1.sup.4 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe ThrSEQ ID No. 153 FR2.sup.4 Trp Val Arg Gln Ala Pro Gly Gln Arg Leu Glu Trp Met GlySEQ ID No. 156 FR3.sup.4 Lys Val Thr Ile Thr Val Asp Thr Ser Ala Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala ArgSEQ ID No. 149 FR4.sup.4 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser;orSEQ ID No. 152 FR1.sup.4 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe ThrSEQ ID No. 155 FR2.sup.4 Trp Val Arg Gln Ala Pro Gly Gln Arg Leu Glu Trp Ile GlySEQ ID No. 156 FR3.sup.4 Lys Val Thr Ile Thr Val Asp Thr Ser Ala Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala ArgSEQ ID No. 149 FR4.sup.4 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser;______________________________________
- and
- (2) a C region of an H chain of a human antibody C.gamma..
Priority Claims (2)
Number |
Date |
Country |
Kind |
3-95476 |
Apr 1991 |
JPX |
|
4-32084 |
Feb 1992 |
JPX |
|
Parent Case Info
This application is a division, of application Ser. No. 08/137,117, filed Dec. 20, 1993 now abandoned, which is the national stage of PCT/JP92/00544, filed Apr. 24, 1992.
Foreign Referenced Citations (2)
Number |
Date |
Country |
9007861 |
Jul 1990 |
WOX |
9109967 |
Jul 1991 |
WOX |
Non-Patent Literature Citations (5)
Entry |
Oi et al. Biotechniques vol. No. 3 1986 p. 214. |
Kitani et al. Clin Exp. Immunol. 88, 75-83, 1992. |
Suzuki et al. Immunology Letters 30 (199) 17-22. |
Morrison Hospital Practice Oct. 15, 1989 p. 65. |
Hirata et al Journal of Immunology 1989, vol. 143 p. 2900. |
Divisions (1)
|
Number |
Date |
Country |
Parent |
137117 |
Dec 1993 |
|