Claims
- 1. A DNA molecule encoding a peptide which inhibits the signaling for the cytotoxic effect by the p75 TNF receptor but does not block TNF binding to the p75 TNF receptor, said peptide comprising the antigen binding portion of an antibody which binds to an extracellular domain of the C-terminal cysteine loop of the p75 TNF receptor, which loop consists of the amino acid sequence Cys-185 to Thr-201 of SEQ ID NO:3, with the proviso that said antigen binding portion is not that of a monoclonal antibody from clones 32 or 70, subcultures of which were deposited as CNCM No. I-1358 or CNCM No. I-928, respectively.
- 2. A DNA hybridizing under moderately stringent conditions to a DNA molecule according to claim 1 and capable of expressing a peptide which inhibits the signaling for the cytotoxic effect by the p75 TNF receptor but does not block TNF binding to the p75 TNF receptor.
- 3. A replicable expression vehicle comprising a DNA molecule according to claim 1, and capable, in a transformant host cell, of expressing said ligand.
- 4. A replicable expression vehicle comprising a DNA molecule according to claim 2, and capable, in a transformant host cell, of expressing said ligand.
- 5. A host transformed with the replicable expression vehicle of claim 3
- 6. A host transformed with the replicable expression vehicle of claim 4.
- 7. A host according to claim 5, wherein said host is prokaryotic.
- 8. A host according to claim 6, wherein said host is prokaryotic.
- 9. An immunoassay for the human TNF Binding Protein TBP-II in body fluids, comprising measuring the interaction of TBP-II (residues 27-214 of SEQ ID NO:2) with an antibody to human TBP-II which specifically recognizes said protein.
- 10. A diagnostic assay for measuring the levels of antibodies to TBP-II endogenously produced in sera of patients in several disorders, comprising measuring the interaction of endogenous antibody with TBP-II.
- 11. A method for the purification of human TNF Binding Protein TBP-II (residues 27-214 of SEQ ID NO:2) utilizing a suitable antibody to human TBP-II which specifically recognizes said protein, comprising the following steps:
a. coupling said antibody to a suitable resin to construct an immunoaffinity column; b. loading a solution containing said protein on said immunoaffinity column; c. washing away the non-bound proteins with a suitable washing buffer; d. eluting the bound TNF Binding Protein TBP-II with a suitable eluent; and e. collecting the enriched fraction of said TBP-II.
Priority Claims (5)
Number |
Date |
Country |
Kind |
90339 |
May 1989 |
IL |
|
91229 |
Aug 1989 |
IL |
|
94039 |
Apr 1990 |
IL |
|
103051 |
Sep 1992 |
IL |
|
106271 |
Jul 1993 |
IL |
|
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] The present application is a division of U.S. application Ser. No. 08/477,347, filed Jun. 7, 1995, which is a continuation-in-part of U.S. application Ser. No. 07/930,443, filed on Aug. 19, 1992, and U.S. application Ser. No. 08/450,972, filed May 25, 1995, now abandoned. The entire contents of both of said applications are hereby incorporated herein by reference. Application Ser. No. 07/930,443, filed on Aug. 19, 1992, is a continuation of application Ser. No. 07/524,263, filed May 16, 1990, now abandoned. Application Ser. No. 08/450,972, filed May 25, 1995, is a continuation of application Ser. No. 08/115,685, filed Sep. 3, 1993, now abandoned.
Divisions (1)
|
Number |
Date |
Country |
Parent |
08477347 |
Jun 1995 |
US |
Child |
09800908 |
Mar 2001 |
US |
Continuation in Parts (2)
|
Number |
Date |
Country |
Parent |
07930443 |
Aug 1992 |
US |
Child |
08477347 |
Jun 1995 |
US |
Parent |
08450972 |
May 1995 |
US |
Child |
08477347 |
Jun 1995 |
US |